LOCUS       DAQSWQ010000001        88069 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.001, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000001 DAQSWQ010000000
VERSION     DAQSWQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.001"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="R8G18_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="R8G18_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HEE9793819.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="R8G18_000002"
     tRNA            1230..1305
                     /locus_tag="R8G18_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="R8G18_000003"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="R8G18_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEE9793820.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="R8G18_000004"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="R8G18_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEE9793821.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="R8G18_000005"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="R8G18_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEE9793822.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="R8G18_000006"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="R8G18_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEE9793823.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="R8G18_000007"
     CDS             complement(4868..5305)
                     /locus_tag="R8G18_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEE9793824.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="R8G18_000008"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="R8G18_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="R8G18_000009"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="R8G18_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HEE9793825.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="R8G18_000010"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="R8G18_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HEE9793826.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8379..9080
                     /gene="pduB"
                     /locus_tag="R8G18_000011"
     CDS             8379..9080
                     /gene="pduB"
                     /locus_tag="R8G18_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001777285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HEE9793827.1"
                     /translation="MAEKSCTLTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSI
                     GILGARIGAGPHIMAADEAVKATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKR
                     GIEVALKELDRTFGDVYANEAGHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAV
                     GVLMADTALKSANVDVVAYSSPAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVL
                     STLGDTPKNDHPSYI"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="R8G18_000012"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="R8G18_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HEE9793828.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="R8G18_000013"
     CDS             10773..11441
                     /locus_tag="R8G18_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HEE9793829.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="R8G18_000014"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="R8G18_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HEE9793830.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="R8G18_000015"
     CDS             11984..13816
                     /locus_tag="R8G18_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HEE9793831.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="R8G18_000016"
     CDS             13806..14156
                     /locus_tag="R8G18_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HEE9793832.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="R8G18_000017"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="R8G18_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HEE9793833.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="R8G18_000018"
     CDS             14475..14897
                     /locus_tag="R8G18_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEE9793834.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="R8G18_000019"
     CDS             14900..15532
                     /locus_tag="R8G18_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HEE9793835.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="R8G18_000020"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="R8G18_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HEE9793836.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="R8G18_000021"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="R8G18_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HEE9793837.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17311
                     /gene="pduO"
                     /locus_tag="R8G18_000022"
                     /pseudo
     CDS             16311..17311
                     /gene="pduO"
                     /locus_tag="R8G18_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
     gene            17308..18693
                     /gene="pduP"
                     /locus_tag="R8G18_000023"
                     /pseudo
     CDS             17308..18693
                     /gene="pduP"
                     /locus_tag="R8G18_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18704..19816
                     /locus_tag="R8G18_000024"
     CDS             18704..19816
                     /locus_tag="R8G18_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEE9793838.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            19813..21156
                     /locus_tag="R8G18_000025"
     CDS             19813..21156
                     /locus_tag="R8G18_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEE9793839.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            21159..21713
                     /gene="pduT"
                     /locus_tag="R8G18_000026"
     CDS             21159..21713
                     /gene="pduT"
                     /locus_tag="R8G18_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HEE9793840.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21713..22063
                     /gene="pduU"
                     /locus_tag="R8G18_000027"
     CDS             21713..22063
                     /gene="pduU"
                     /locus_tag="R8G18_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HEE9793841.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22068..22511
                     /locus_tag="R8G18_000028"
     CDS             22068..22511
                     /locus_tag="R8G18_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HEE9793842.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22612..22941)
                     /locus_tag="R8G18_000029"
     CDS             complement(22612..22941)
                     /locus_tag="R8G18_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HEE9793843.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23113..24171)
                     /locus_tag="R8G18_000030"
     CDS             complement(23113..24171)
                     /locus_tag="R8G18_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEE9793844.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24369..24842)
                     /gene="sbmC"
                     /locus_tag="R8G18_000031"
     CDS             complement(24369..24842)
                     /gene="sbmC"
                     /locus_tag="R8G18_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HEE9793845.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24961..26127)
                     /gene="dacD"
                     /locus_tag="R8G18_000032"
     CDS             complement(24961..26127)
                     /gene="dacD"
                     /locus_tag="R8G18_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HEE9793846.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26336..27763
                     /gene="sbcB"
                     /locus_tag="R8G18_000033"
     CDS             26336..27763
                     /gene="sbcB"
                     /locus_tag="R8G18_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HEE9793847.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27806..28033)
                     /locus_tag="R8G18_000034"
     CDS             complement(27806..28033)
                     /locus_tag="R8G18_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEE9793848.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28047..29105)
                     /gene="yeeE"
                     /locus_tag="R8G18_000035"
     CDS             complement(28047..29105)
                     /gene="yeeE"
                     /locus_tag="R8G18_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HEE9793849.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29284..30642)
                     /gene="plaP"
                     /locus_tag="R8G18_000036"
     CDS             complement(29284..30642)
                     /gene="plaP"
                     /locus_tag="R8G18_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HEE9793850.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30632..30694)
                     /gene="yoeI"
                     /locus_tag="R8G18_000037"
     CDS             complement(30632..30694)
                     /gene="yoeI"
                     /locus_tag="R8G18_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HEE9793851.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30909..31838)
                     /locus_tag="R8G18_000038"
     CDS             complement(30909..31838)
                     /locus_tag="R8G18_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9793852.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31884..32708)
                     /locus_tag="R8G18_000039"
     CDS             complement(31884..32708)
                     /locus_tag="R8G18_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEE9793853.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32791..33045)
                     /gene="yoeB"
                     /locus_tag="R8G18_000040"
     CDS             complement(32791..33045)
                     /gene="yoeB"
                     /locus_tag="R8G18_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HEE9793854.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33042..33293)
                     /gene="yefM"
                     /locus_tag="R8G18_000041"
     CDS             complement(33042..33293)
                     /gene="yefM"
                     /locus_tag="R8G18_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HEE9793855.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33576..33626
                     /gene="hisL"
                     /locus_tag="R8G18_000042"
     CDS             33576..33626
                     /gene="hisL"
                     /locus_tag="R8G18_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HEE9793856.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33602..33725
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33772..34671
                     /gene="hisG"
                     /locus_tag="R8G18_000043"
     CDS             33772..34671
                     /gene="hisG"
                     /locus_tag="R8G18_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEE9793857.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34677..35981
                     /gene="hisD"
                     /locus_tag="R8G18_000044"
     CDS             34677..35981
                     /gene="hisD"
                     /locus_tag="R8G18_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEE9793858.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35978..37048
                     /gene="hisC"
                     /locus_tag="R8G18_000045"
     CDS             35978..37048
                     /gene="hisC"
                     /locus_tag="R8G18_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEE9793859.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37048..38115
                     /gene="hisB"
                     /locus_tag="R8G18_000046"
     CDS             37048..38115
                     /gene="hisB"
                     /locus_tag="R8G18_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HEE9793860.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38115..38705
                     /gene="hisH"
                     /locus_tag="R8G18_000047"
     CDS             38115..38705
                     /gene="hisH"
                     /locus_tag="R8G18_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEE9793861.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38705..39442
                     /gene="hisA"
                     /locus_tag="R8G18_000048"
     CDS             38705..39442
                     /gene="hisA"
                     /locus_tag="R8G18_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEE9793862.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39424..40200
                     /gene="hisF"
                     /locus_tag="R8G18_000049"
     CDS             39424..40200
                     /gene="hisF"
                     /locus_tag="R8G18_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEE9793863.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40194..40805
                     /locus_tag="R8G18_000050"
     CDS             40194..40805
                     /locus_tag="R8G18_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEE9793864.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40902..41879)
                     /gene="wzzB"
                     /locus_tag="R8G18_000051"
     CDS             complement(40902..41879)
                     /gene="wzzB"
                     /locus_tag="R8G18_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HEE9793865.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42025..43191)
                     /gene="ugd"
                     /locus_tag="R8G18_000052"
     CDS             complement(42025..43191)
                     /gene="ugd"
                     /locus_tag="R8G18_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEE9793866.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43441..44847)
                     /gene="gndA"
                     /locus_tag="R8G18_000053"
     CDS             complement(43441..44847)
                     /gene="gndA"
                     /locus_tag="R8G18_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEE9793867.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45011..46381)
                     /gene="cpsG"
                     /locus_tag="R8G18_000054"
                     /gene_synonym="manB"
     CDS             complement(45011..46381)
                     /gene="cpsG"
                     /locus_tag="R8G18_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HEE9793868.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46486..47922)
                     /gene="cpsB"
                     /locus_tag="R8G18_000055"
     CDS             complement(46486..47922)
                     /gene="cpsB"
                     /locus_tag="R8G18_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HEE9793869.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47925..49148)
                     /gene="wcaI"
                     /locus_tag="R8G18_000056"
     CDS             complement(47925..49148)
                     /gene="wcaI"
                     /locus_tag="R8G18_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HEE9793870.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49145..49624)
                     /gene="gmm"
                     /locus_tag="R8G18_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49145..49624)
                     /gene="gmm"
                     /locus_tag="R8G18_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HEE9793871.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49627..50592)
                     /gene="fcl"
                     /locus_tag="R8G18_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49627..50592)
                     /gene="fcl"
                     /locus_tag="R8G18_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEE9793872.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50595..51716)
                     /gene="gmd"
                     /locus_tag="R8G18_000059"
     CDS             complement(50595..51716)
                     /gene="gmd"
                     /locus_tag="R8G18_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEE9793873.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51742..52290)
                     /gene="wcaF"
                     /locus_tag="R8G18_000060"
     CDS             complement(51742..52290)
                     /gene="wcaF"
                     /locus_tag="R8G18_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HEE9793874.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52306..53052)
                     /gene="wcaE"
                     /locus_tag="R8G18_000061"
     CDS             complement(52306..53052)
                     /gene="wcaE"
                     /locus_tag="R8G18_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HEE9793875.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53063..54280)
                     /gene="wcaD"
                     /locus_tag="R8G18_000062"
     CDS             complement(53063..54280)
                     /gene="wcaD"
                     /locus_tag="R8G18_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HEE9793876.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54255..55472)
                     /gene="wcaC"
                     /locus_tag="R8G18_000063"
     CDS             complement(54255..55472)
                     /gene="wcaC"
                     /locus_tag="R8G18_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HEE9793877.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55469..55957)
                     /gene="wcaB"
                     /locus_tag="R8G18_000064"
     CDS             complement(55469..55957)
                     /gene="wcaB"
                     /locus_tag="R8G18_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HEE9793878.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55960..56799)
                     /gene="wcaA"
                     /locus_tag="R8G18_000065"
     CDS             complement(55960..56799)
                     /gene="wcaA"
                     /locus_tag="R8G18_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HEE9793879.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56892..59054)
                     /gene="wzc"
                     /locus_tag="R8G18_000066"
     CDS             complement(56892..59054)
                     /gene="wzc"
                     /locus_tag="R8G18_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HEE9793880.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59057..59500)
                     /gene="wzb"
                     /locus_tag="R8G18_000067"
     CDS             complement(59057..59500)
                     /gene="wzb"
                     /locus_tag="R8G18_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HEE9793881.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59506..60645)
                     /gene="wza"
                     /locus_tag="R8G18_000068"
     CDS             complement(59506..60645)
                     /gene="wza"
                     /locus_tag="R8G18_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HEE9793882.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60954..61103
                     /locus_tag="R8G18_000069"
     CDS             60954..61103
                     /locus_tag="R8G18_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9793883.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61304..62887
                     /locus_tag="R8G18_000070"
     CDS             61304..62887
                     /locus_tag="R8G18_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEE9793884.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63161..65014)
                     /gene="asmA"
                     /locus_tag="R8G18_000071"
     CDS             complement(63161..65014)
                     /gene="asmA"
                     /locus_tag="R8G18_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HEE9793885.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(65036..65617)
                     /gene="dcd"
                     /locus_tag="R8G18_000072"
     CDS             complement(65036..65617)
                     /gene="dcd"
                     /locus_tag="R8G18_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEE9793886.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65709..66350)
                     /gene="udk"
                     /locus_tag="R8G18_000073"
     CDS             complement(65709..66350)
                     /gene="udk"
                     /locus_tag="R8G18_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEE9793887.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66668..69985
                     /locus_tag="R8G18_000074"
     CDS             66668..69985
                     /locus_tag="R8G18_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEE9793888.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70095..70943)
                     /gene="alkA"
                     /locus_tag="R8G18_000075"
     CDS             complement(70095..70943)
                     /gene="alkA"
                     /locus_tag="R8G18_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HEE9793889.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71077..72429
                     /gene="yegD"
                     /locus_tag="R8G18_000076"
     CDS             71077..72429
                     /gene="yegD"
                     /locus_tag="R8G18_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEE9793890.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72442..74382)
                     /gene="yegI"
                     /locus_tag="R8G18_000077"
     CDS             complement(72442..74382)
                     /gene="yegI"
                     /locus_tag="R8G18_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HEE9793891.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74379..75140)
                     /gene="pphC"
                     /locus_tag="R8G18_000078"
     CDS             complement(74379..75140)
                     /gene="pphC"
                     /locus_tag="R8G18_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HEE9793892.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75137..75796)
                     /locus_tag="R8G18_000079"
     CDS             complement(75137..75796)
                     /locus_tag="R8G18_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEE9793893.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76017..76076)
                     /gene="ibsA"
                     /locus_tag="R8G18_000080"
     CDS             complement(76017..76076)
                     /gene="ibsA"
                     /locus_tag="R8G18_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HEE9793894.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76349..76405)
                     /locus_tag="R8G18_000081"
     CDS             complement(76349..76405)
                     /locus_tag="R8G18_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HEE9793895.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76676..76732)
                     /locus_tag="R8G18_000082"
     CDS             complement(76676..76732)
                     /locus_tag="R8G18_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HEE9793896.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77011..78252
                     /gene="mdtA"
                     /locus_tag="R8G18_000083"
     CDS             77011..78252
                     /gene="mdtA"
                     /locus_tag="R8G18_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HEE9793897.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78252..81374
                     /gene="mdtB"
                     /locus_tag="R8G18_000084"
     CDS             78252..81374
                     /gene="mdtB"
                     /locus_tag="R8G18_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HEE9793898.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81375..84452
                     /gene="mdtC"
                     /locus_tag="R8G18_000085"
     CDS             81375..84452
                     /gene="mdtC"
                     /locus_tag="R8G18_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HEE9793899.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLLSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84453..85868
                     /locus_tag="R8G18_000086"
                     /pseudo
     CDS             84453..85868
                     /locus_tag="R8G18_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            85865..87268
                     /gene="baeS"
                     /locus_tag="R8G18_000087"
     CDS             85865..87268
                     /gene="baeS"
                     /locus_tag="R8G18_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HEE9793900.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87265..87987
                     /gene="baeR"
                     /locus_tag="R8G18_000088"
     CDS             87265..87987
                     /gene="baeR"
                     /locus_tag="R8G18_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HEE9793901.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21550 a        23311 c        22149 g        21059 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgat agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaaacgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa ggcaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccgat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcgg ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcatgc atttcgcccg
    16681 cacggtggct cgacgggctg aaagacggct ggttgaactg acaacggaaa ccaccgtccg
    16741 gaatgttttg ctgcactata tcaaccgact ttccgattgc ctgtatgccc tcgcccgcgt
    16801 ggaagacaac gttgctcatc aaaacctgat gattcaggaa atcacaaaac gttatcacga
    16861 agccaaccac acaccagcat tgaaggaacg cacaatgccg ctcacttttc aggatcttca
    16921 ccagctcatt cgtagtgcgg caatgcgcgc ggatgaactg catattcccg ttgtcatcag
    16981 cattgttgat gccaacggaa cagaaagtgt tacctggcgg atgcctgatg ccctgttggt
    17041 cagcagtgaa ctggcaccga aaaaagcctg gaccgccgtg gcgatgaaaa cggcaaccca
    17101 caagcttgct gataccgtgc aacccgacgc gccgctttac gggctcgaaa gccatatgca
    17161 aggaaaagtc gtcacttttg gtggcggctt ccccctctgg cgtgacggaa aattgcttgg
    17221 cgggcttggc atcagcggcg gtagcgttga acaagacatg gatattgccc aaagcgcaat
    17281 ggcggcaatt aacgtgggag taaaccaatg aataactctg aattagaaac actgatccgc
    17341 aatattctgc gtgagcaact tgtccccgct acaccacgaa cgcaaagaaa cgctattttc
    17401 cagactgtcg atgaagcggt ctgcgcagca cactaggcct ttttacgctt tcaacagtgc
    17461 ccgcttaaaa cccgtagcgc cattatcagc gccattcgcg aagaattaat gccccgcctt
    17521 accgaactgg cagaagaaag cgccaaagaa acggggatgg gaaataaaga agataagtac
    17581 ctaaaaaaca aagcggcact cgataacacg ccaggaattg aagatttaac caccacagca
    17641 ctaaccggtg atggtggcat ggttttgttt gagtactctc cgtttggggt gattggctcg
    17701 gtcacgccaa gcaccaaccc cacggaaacc atcattaaca acagcatcag catgctggca
    17761 gcaggtaata gcgtctactt cagtccacac ccgggtgcca aaaacgtttc gttaaaactg
    17821 attaccacaa tcgaagacat cgcgtttcgc tgtagtggca ttcataactt gatcgttacc
    17881 gtatctgaac cgacctttgc tgccaccgaa cagatgatgg cgcaccctaa aatcagcgtt
    17941 ctggcgatta ccggcggtcc ggggatcgtc gccatgggaa tgaaaagtgg caagaaagtg
    18001 attggtgccg gagcgggtaa ccctccgtgc atcgtcgatg aaacggcgga tattgtgaaa
    18061 gccgccgagg acatcatcaa cggcgcttca tttgattaca acctgccgtg tattgctgaa
    18121 aaaagcctga ttgtcgtggc atcagtggca gattatttaa ttcagcagat gcaaagtttt
    18181 ggcgcacttc ttcttaacta tgagcaaact gagaaattgc gtgcaatttg cctgccagac
    18241 ggtagcgcca acaaaaaact ggttgggaaa agcccatcag ccttgctgga agcggcaggc
    18301 ctccctttgc ccgcaaaagc gccgcgccta ctgattgctg ttgtcgacgc cgatgatagt
    18361 tgggttactt gtgagcaact aatgccaatg ctgcccattg tgaaggtcaa ggattttgac
    18421 agcgctctaa ccctggcgct gaaagtggaa gacggcttgc accacacggc aattatgcac
    18481 tctcagaacg tctcacgact caaccttgcc gcgcgcgtta tgcaaacctc aatatttgtc
    18541 aaaaacggcc cctcctgggc gggtattggc gtcggcggcg aaggctttac gacctttacc
    18601 attgcaacac cgactggaga gggtacaacg tcagcacgga cttttgcccg ctcacgacgc
    18661 tgcgtgttaa ccagcggctt ttcaattcgc tagagaaacg gaaatgaata ctttttcact
    18721 gcagacgcgt ttatacagcg gaccaggaag tctcaaggta cttagccgtt tctctcatag
    18781 acacatctgg attatttgcg atgcctttct ggcacgctca ccacttattg acacgttgca
    18841 tcaggcgctg cccgacagta atcgtctcag tatttttagc gaaataacgc ccgccccaac
    18901 tatccagacc gtggtgaaag gcattgcgca aatgcagact ttacgcccgg atgtagttat
    18961 cggttttggc ggcggttctg ccctggacgc cgcaaaagca attgtctggt tcggtcgcca
    19021 gtgtggcatt gaaattgaaa cctgcgtagc cattcctacc accagcggta tcggttccga
    19081 agtgaccagt gcttgtgtca tcagcgatcc tgagaaaggg attaaatatc cccttttcga
    19141 taatgcactt tatccggata tcgcgatcct tgatccatct ttggtcgtca gcgtgccacc
    19201 ggcgataacg gccaataccg ggatggatgt tttaacccat gcgcttgaag cttatgtttc
    19261 tcctcgcgcc agcgacttca cggatgcact ggtcgaaaaa gccgtgcaaa ttgtttttca
    19321 atacctgccc actgcagtga aaaaaggcga ttgtcttgcc acccgtggca aaatgcataa
    19381 tgcatcgacg cttgcaggaa tggcctttag ccaggccgga ctcggtttaa accatgcaat
    19441 tgctcatcaa ctcggcggac agtttcatct gccgcatgga ctggcgaatg cgctcctgct
    19501 cacggcagtt atccgcttta atgctggcga tccgcgcaca gccaaacgct atgcccggct
    19561 ggcgaaaacg tgccatttat gcccggacaa cgccaatgac accgccagcc tgaatgcact
    19621 tattcaacat attgagcagc ttaatacaac ctgtacgctg ccgactcttg ccaatgcgct
    19681 gaaagaaaaa aaagcagaat ggtcaatacg cataccagat atggttcagg cggcgctggc
    19741 ggatgcaacg ttgcgtacca acccgcgtgc agctgatgcc tccgcgattg cagaactgct
    19801 cgaggagttg ttatgaacgt cggattaatc tcctgcgacg accagaccat ccgcgagcga
    19861 gtacaagcag caggtgtcgt gggtgctggc ggcgcaggtt ttccaaccca tattaagcta
    19921 caagcgcgtg ctgacacttt cctggtgaat gcggcggaat gcgaacccat gttgaaagta
    19981 gatcaacagt taatggcgca gcaagccagc cgcctgcttc gcggtgttca ctatgcgatg
    20041 aaagccaccg gggccagtag cggaatcatc gcgctgaaag aaaaatatca acgggcaata
    20101 aacgcactca caccactgtt accaccggat atccgcctgc atattttgcc cgacgtttac
    20161 cccgcaggag acgaagtgct gaccatctgg atggcaactg gtcgccgcgt tccacccgct
    20221 gcattacccg ttagcgtcgg tgtggtggtc aataatgttc aaaccgtact taatattgcc
    20281 agagccgtcg agcaacagta tccagtgacg cgccgcacac tcaccgttaa tggtgcagtg
    20341 gcgaagccaa taacggtaac cgtccccatc ggcatgtctt tacgtgaagt cctggcgctg
    20401 gcgggcggcg caacagtgga tgctcctggt tttatcaatg gcgggccgat gatgggaagt
    20461 ttgatcacat cgctcgacac accagtcagc aaaaccactg gcggcttgct ggttttaccc
    20521 aactctcatt cactgatcca acgtcgaatg cagaatgacc gcagcgtact tgccgtcgcg
    20581 aaaactgttt gtgagcaatg ccgcttgtgt accgatctct gcccacgtca tttaatcggt
    20641 cacgaactgg caccccattt attagtacgt gcggttaact accaacagct cgccacgccg
    20701 caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag tgtcgcctgt
    20761 ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt acgcgcacaa
    20821 aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta tcgactgatt
    20881 cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga tgcgccattg
    20941 tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca catcggtgcc
    21001 agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg tgtaggtcaa
    21061 attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat ggtcagcgat
    21121 gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg gtaggtattt
    21181 tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg aaaagcgcca
    21241 acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg atgctcggtg
    21301 gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct ggtgaaatgc
    21361 tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca atcagcggac
    21421 ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt gtcgccgcct
    21481 gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt cgcgtacata
    21541 tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt tcagatgtta
    21601 ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg gtctatcggt
    21661 cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga taatgacaac
    21721 tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg tcacgctggc
    21781 acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac cagacaccat
    21841 ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg catgcgatat
    21901 cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg gtgccgtagt
    21961 gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc gtacgctggg
    22021 tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg caacgtatga
    22081 tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg cgtggcgaaa
    22141 atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt gatacaccag
    22201 gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc tgcgaagcgg
    22261 acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg ccaggcttta
    22321 cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta gccaacatgg
    22381 aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac gtgttagtaa
    22441 ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat acagaagaag
    22501 gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac acagaaaaga
    22561 atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga attacttcgc
    22621 ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc ggcgttcttt
    22681 ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt cacctttcat
    22741 gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt aatcgctcag
    22801 gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa cgtcctgaat
    22861 ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta cgtcctggaa
    22921 tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcag attcgtttaa
    22981 gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac aatgctttta
    23041 tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca agatgattct
    23101 gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca actcaagctg
    23161 atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca ctaccttcag
    23221 gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca atttttccgt
    23281 attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc tcagcagtat
    23341 ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca cgaaatggct
    23401 ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca gattgcggtt
    23461 aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc tggcgggcgc
    23521 aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc tttccagacg
    23581 tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat actcggtcag
    23641 acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga taccggtaaa
    23701 caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg ccgtatcaat
    23761 ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata atccttgata
    23821 cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc accagactaa
    23881 catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa tcgaacccaa
    23941 caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga acgagattgg
    24001 ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag taaacaggcg
    24061 gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca ctatgcggta
    24121 atggcggtat acccggattt caaacgggct taatgactta tcggcacgca cgcagaaact
    24181 ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg cgcctctgaa
    24241 ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa aaattgaaag
    24301 gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac acaccgccct
    24361 gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc ccagtaccca
    24421 tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat ttcataagca
    24481 ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc atcaccgact
    24541 acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat gacgccctca
    24601 ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca gcgtaatttt
    24661 tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt cggcacaata
    24721 tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt ctgttcccac
    24781 gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat ctcgtagttc
    24841 atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta aaaccacaag
    24901 gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc aaaagaaagg
    24961 tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt ccccgacaga
    25021 ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa gttcaatttc
    25081 ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa gggtatattt
    25141 ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt gttccgttcc
    25201 taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct ttttcccacg
    25261 gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt ttcttgcctc
    25321 ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac gacgctgccc
    25381 atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg ttttcaggcc
    25441 gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg tgataccgtt
    25501 ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt ggatgatagc
    25561 gcgagaaagc acagctaaat cataagccga gctatgctgg ccaggtgcat ccagaccatg
    25621 cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt tcatcatttc
    25681 aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag cgtcatttcc
    25741 ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc cctctttcaa
    25801 aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat cgcgccccac
    25861 ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca cgacataacc
    25921 cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac ccgcggtgag
    25981 gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct ctggaggctg
    26041 aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa cgaacaaaga
    26101 agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa atagctgttc
    26161 tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt gactaccctc
    26221 tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc taaagctgat
    26281 tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta acctaatgat
    26341 gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct ttggcacgca
    26401 ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgatagcg aattcaatgt
    26461 catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac cccagccagg
    26521 agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag aaaacgaagc
    26581 cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta ttctgggcta
    26641 caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta atttctacga
    26701 tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg atgttatgcg
    26761 tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg acggtctacc
    26821 gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca acgcccacga
    26881 tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa cgcgtcagcc
    26941 acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg cgttgattga
    27001 tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat ggcgcggcaa
    27061 taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg taattatggt
    27121 ggatttggca ggagacattt cgccattact ggaactggat agcgacacat tgcgcgagcg
    27181 tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta agctggtgca
    27241 tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag atgccgaccg
    27301 actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg aaaatccgca
    27361 agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc cttcagataa
    27421 cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag caatgaaaat
    27481 tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg tcgataaacg
    27541 gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc tggattatgc
    27601 cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt ttttacaagg
    27661 ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag agaaagtggc
    27721 gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag cctccgccgc
    27781 tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc cacgctgcat
    27841 cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac tgtgggattg
    27901 cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg gccatttctg
    27961 ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc acatccagct
    28021 ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag tcgcgcctta
    28081 cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat cataaataca
    28141 aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt accgataaag
    28201 caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc gctacgtaat
    28261 gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc tgcaataaat
    28321 gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt cacgtcaccc
    28381 gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc aaacatgcgt
    28441 ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag tacagcagtg
    28501 acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg gcgcggcggt
    28561 aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca gagcgttatc
    28621 accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc aatagagtta
    28681 tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg agaacgcatc
    28741 accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa tccctcaccc
    28801 gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag cccgaagata
    28861 tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt cagtaggccc
    28921 gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag caaggcgtaa
    28981 aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag gcaaaaacgc
    29041 ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa tatcattgaa
    29101 aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac tggtggaatg
    29161 cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg atttcgataa
    29221 aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta ttagaaaaga
    29281 tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg tgacgcaagc
    29341 caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc tttcttccag
    29401 gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca gatactggaa
    29461 gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga tgaccgacag
    29521 gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag ccattaccag
    29581 gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca tcgccggagt
    29641 acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt cacgacccat
    29701 tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca gtacggtaat
    29761 ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca gcatgatttc
    29821 aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca gctgcaggaa
    29881 gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa tcgcacgcgg
    29941 gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt caaagccggt
    30001 aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg catcgccaga
    30061 ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata cgccataaac
    30121 aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta cagtgttgaa
    30181 gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca ccagcgccac
    30241 cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt tcgccagcag
    30301 aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca ccataaagcc
    30361 gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag aaggatagcg
    30421 gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg cgaacgcata
    30481 ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat caaacagcgt
    30541 catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa gcgttttacg
    30601 caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc tcctttacgg
    30661 tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca gcgacaacaa
    30721 ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt atgaatgatt
    30781 ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca ttttttgaca
    30841 gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct tgtcataata
    30901 atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc ggcgatcgca
    30961 ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt ctgttcgttc
    31021 cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc gattaacgcg
    31081 ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc gaggccgcta
    31141 atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg ttcgtcgaga
    31201 agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat cgcccgatat
    31261 cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg atgaggcgcg
    31321 accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact ggaagatggt
    31381 ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc atcccaggaa
    31441 ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg ataaaaagct
    31501 tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat caccagctcg
    31561 ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac ggtatgcaga
    31621 acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc gcggtgaccg
    31681 ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt gtaactcaga
    31741 gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc tttttctaag
    31801 gcatcgagga tcatcaacac atccagcagt tgtttcatac acgccccctt gcagtgcatg
    31861 cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc gggatactgg
    31921 tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc tttgccgctg
    31981 tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat ctgcggatag
    32041 aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc gcctttaggt
    32101 gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa attaacacca
    32161 tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg tcccggtccg
    32221 accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg cagccagtct
    32281 tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt ggtttctttc
    32341 accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat gcgaggaata
    32401 cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa ctcatcgccg
    32461 gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat cagggcatcc
    32521 agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct atcaatgcca
    32581 ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac ttgccagcct
    32641 cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac aattgcgacc
    32701 tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta gcgtatcaaa
    32761 actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg ctgcaatgag
    32821 cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc gtcgggacca
    32881 gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa atggcgttct
    32941 gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg tttcctgcca
    33001 gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat aatgtccttt
    33061 tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg ggcgttagcg
    33121 ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag tgacatcaga
    33181 acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc aacggctttc
    33241 atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg catgtcaatc
    33301 tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt attaattagt
    33361 ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt ttggtgccgt
    33421 cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt aaacaaatcc
    33481 attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt ttaggttaaa
    33541 agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa tttaaacacc
    33601 accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa gacattcaga
    33661 tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc cgggggcttt
    33721 tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa aatgacagac
    33781 aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga ctcacgcgaa
    33841 ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat cgcgatggca
    33901 gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg tctggtaatg
    33961 gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga gctgcttaac
    34021 cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga tttcggcggc
    34081 tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct ctccttaaac
    34141 ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga ccagaaaggc
    34201 atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg tgccggactg
    34261 gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa cggcctgcgc
    34321 gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg tgaaatggaa
    34381 gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt gatccaggcg
    34441 cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga agtcatcgcc
    34501 ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca acaacgcgta
    34561 gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa actgaaagcg
    34621 ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg atcgccatga
    34681 gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc cagctgttaa
    34741 tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat attctcgata
    34801 acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt gataaaacca
    34861 cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa cgcctgagcg
    34921 acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc cacactgcgc
    34981 aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag caggtcacgc
    35041 gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc ttctcaacgg
    35101 tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg ctgtgctcac
    35161 cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg aaggacgtgt
    35221 ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa tctgtgccga
    35281 aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa cgtcaggtga
    35341 gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa gtgttggtca
    35401 ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct caggctgaac
    35461 acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg catcaagttg
    35521 ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc cgccaggcac
    35581 tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag atctccaacc
    35641 agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg gtcgatggca
    35701 tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca ggtgattacg
    35761 cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt tccagcctcg
    35821 ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa ggtttctccg
    35881 cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc cataaaaatg
    35941 ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga ctattaccga
    36001 tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc gtctgggcgg
    36061 taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt ttcagcttac
    36121 tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg aaaattacgc
    36181 gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg acgaaggtat
    36241 tgaactgctg attcgcgctt tttgcgaacc gggtaaagac gccatcctct actgcccgcc
    36301 aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc gcacagtgcc
    36361 gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg acggcgtaaa
    36421 agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc cgcaggattt
    36481 tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg aagcctatat
    36541 cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc acctggctat
    36601 tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat ttacgctggc
    36661 aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc tctcgacgcc
    36721 ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta tgcgcgaacg
    36781 agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag agatcccctg
    36841 cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta aagcctccag
    36901 cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga ataaacaacc
    36961 ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc agcgcgtcat
    37021 tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat cgatcgcgat
    37081 ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga caaactcgcc
    37141 tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta caaactggta
    37201 atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga tttcgatggc
    37261 ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga tgaagtgctg
    37321 atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt aaaactggtg
    37381 gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat tggcgatcgc
    37441 gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta cgaccgcgaa
    37501 atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta cgctcacgta
    37561 gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg cgaaggtggc
    37621 agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat cgccacccac
    37681 ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga tcaccacacc
    37741 gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg cgacaaacgc
    37801 ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg ctgcgcgctg
    37861 gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca gcgcgtgggc
    37921 gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac catgggcgtg
    37981 acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag cctgttcaaa
    38041 gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct gccctcgtcg
    38101 aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat ctgaactcgg
    38161 tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac ccggacgtgg
    38221 tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca gcgatggatc
    38281 aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg gtactgggca
    38341 tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc gtcgacttgc
    38401 tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg ctgccgcata
    38461 tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg attgaagacg
    38521 gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg accattgccc
    38581 aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc tacggcgtgc
    38641 agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac ttcctggaga
    38701 tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc tccatcaggg
    38761 cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac aggattacgc
    38821 cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag atccggcaaa
    38881 acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg tgcaggttgg
    38941 cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg ttgcgcgcgt
    39001 ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt ttgaacgttt
    39061 cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag gcaacaagca
    39121 ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac tggtggaaac
    39181 ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg acggcacgct
    39241 ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc aggtggcatt
    39301 tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag gcactggcgt
    39361 gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga aggaggccat
    39421 cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca ggtggtgaaa
    39481 ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc aaaacgctac
    39541 gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag cgatggccgt
    39601 gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc gttctgcgtg
    39661 gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg cgcggataaa
    39721 atttccatca actcccctgc gctggcagac ccaaccttaa ttactcgcct ggcggatcgc
    39781 tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga aaccggtaaa
    39841 tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca gtgggaaaca
    39901 ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct caacatgatg
    39961 aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt gcgtgaagtt
    40021 tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt cctcgaagcc
    40081 ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa acaaataatc
    40141 aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag gatatgttaa
    40201 cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg ccggtaattg
    40261 tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg gaagccttag
    40321 acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag cgactgtgga
    40381 ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg gactgcgaca
    40441 acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa ggcaccagca
    40501 gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa caactgctcg
    40561 ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat gccagcggca
    40621 ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg gcagcgacgg
    40681 tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac ctgctggtgc
    40741 tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt aaacggcatc
    40801 agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa aaaaagggca
    40861 aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc gttataatta
    40921 cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag cagcaccgcc
    40981 agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt cggtttcatc
    41041 acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat atccaacagg
    41101 ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc ctcatgctta
    41161 atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt cacatcttca
    41221 gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa cgcttcctga
    41281 atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt tcttaaagag
    41341 tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc tagctcttga
    41401 ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc tccctctgca
    41461 gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga aggttcgatg
    41521 gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga gaaggcagaa
    41581 ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc ctgaccatag
    41641 ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg agtgataatt
    41701 gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag ggcaatagcc
    41761 acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcactag taaatcaatc
    41821 aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc tactctcatc
    41881 attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc ctagtgaatg
    41941 ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga caatcagggc
    42001 gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg tgtatacctt
    42061 atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt cggcttgttg
    42121 tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt ctttcatcac
    42181 tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa tcccctgaat
    42241 agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa tacccaccac
    42301 ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc ggtttgcatc
    42361 gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta actgcttggt
    42421 atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat ggttgccaat
    42481 acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta aactttctgc
    42541 gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt ttgcaaaaag
    42601 tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt gcttaatcgc
    42661 gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac cgatgacaat
    42721 acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt ccggagagaa
    42781 aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa cgggaaccgt
    42841 tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa ttactgattc
    42901 tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag tggcgatgat
    42961 gacataatca gcatcccggt aggcttcatt tttatctaat gtggcattaa agtgtatttt
    43021 atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc gatcattcag
    43081 catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt gtgcgattag
    43141 aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca tttcatgctc
    43201 tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga gctaaatgtt
    43261 ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagattt tctttataag
    43321 aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata agataaatgg
    43381 caaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttactgttgg ttaaatcaga
    43441 ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat aagtatgtgc
    43501 accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg cacggtagct
    43561 atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct gtactgcata
    43621 agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga agtacggagc
    43681 cagcagcagg ttagcgatct gcggattttc ggcatatgca tcggtgattt tctgcaggaa
    43741 ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt agttcagatc
    43801 ccagttgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt aagaaacgat
    43861 tttgcccaga tacagcgcac ggcgaacttt ttcgatgaac tcagccttgt cgcctgctga
    43921 ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt tcagagaaga
    43981 gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga gatccagcgc
    44041 gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga tcacatcaac
    44101 caggtagtta ccgtcttcat cttttttagt gaagatatct ttggtgatgt cgatcaggta
    44161 gctgctcagt tcaccgttat tccactcggt aaaggtctgt gccagttctt cgttagagag
    44221 attcaggccg cctttcagga gagaataggc ttcagcaatc agttgcatat caccgtattc
    44281 aataccgttg tgaaccatct tcacataatg gcctgcgcca tcagcaccga tataggtaac
    44341 gcacggttcg ccatcttcag caacggcggc gattttggtc aggatcggcg caaccagttc
    44401 ataggcttct ttctggccac caggcatgat ggacggccct ttcagcgccc cctcttctcc
    44461 gccggaaaca ccggtaccga tgaagttaaa gccttcggca gaaagctcac ggttacgacg
    44521 aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt tatcgagata
    44581 tggtttgagc gaatcaatag cagcatccgt gcctgcacct gctttcacca ttaacaggat
    44641 gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag gaaccagttt
    44701 cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt tgaaaataga
    44761 gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca ctgccatacc
    44821 gactacgccg atctgttgct ttgacattgt ttactcctgt cagggggtgg tcaccgccga
    44881 gtgtgcggct taaatgtggt atagatgtta actcactcat agagatggtg aatagcatta
    44941 cctttgctat accacactga atatctcacc ttttccgtct tttaaaactt atttatatga
    45001 aattactgaa ttacttgttc agtaactcaa gaataagttt tgtcttttct tccatcagcg
    45061 gcacatcacc gcgagattcc acattcaacc gcaccaccgg ttcggtgttg gaggtgcgca
    45121 ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc ggtgcgatcc accgccagcg
    45181 cctcgcggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc gccagtttgc
    45241 tgttgatctc accgcttgcc ggaaacgctg ccatccggtc gcgcaccagt tcgcccagcg
    45301 tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg ccgctgtcgc
    45361 agtaagcgaa atcacggaag taatggtggg cgctcatttc accaccgtag atggcgtctt
    45421 ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc ggcgtgccgc
    45481 ctgcggcagt caccacatca acggtgttcc aggagagacg cggatcgtgg atgatcttcg
    45541 cgccgggatt tttttcgagg aatgcttctg ccagcaggcc gacaatgtag tagccctcaa
    45601 taaactgccc tttttcgtca aacaggaagc agcggtcaaa atcgccatca aaagcaatgc
    45661 ccatatccgc accgtgtttg atgaccgcat tgcgggtgtc gtcgcggcat tccggcagta
    45721 gcgggttagg aataccgttg gggaaattgc cgtccggcgt gttgtgcact ttgattaatt
    45781 ccacgggtgc gccaagggct ttaaagcggg cttcgatggc gtccaccacc ggacccgctg
    45841 cgccattccc ggagttaatc accagcttga gcggcgtgag gtttttgaca ttgatataac
    45901 cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga ccgcgtttgg
    45961 tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg cgcagtccgg
    46021 tatcaccgct gatcgggcga gccccttcgc gcaccagttt catgccgttg taatccatcg
    46081 ggttatggct ggcggtaact tcaatgccgc catccacgcc gagatggaac gtggcgaaat
    46141 agatctcttc ggtgccggac atgccaatat ccagcacatc gacgcccgca tcctgtaaac
    46201 ctctcgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg cctaacacaa
    46261 tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg atatcttcat
    46321 tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt aattttttca
    46381 tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat tttgtttttg
    46441 tcgttattta catctcggtt tcgcattcat tgcctgatgc gacgtttaca cccgtccgta
    46501 gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc gcacttcaat
    46561 taaatcgagc ggaattttcc ccgggttttc caggcaatgc atcgccccca gcggaatata
    46621 aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt ttgccgttcc
    46681 cgcgacaacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca agccctcgcc
    46741 cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag agtcatattt
    46801 gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccat cggctttgat
    46861 ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca gcaccgcatc
    46921 tttggtctgc accactacca gatctttcac cccgacggtg gttacgaggc cagattcggc
    46981 gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa cgttgccctc
    47041 ggcggtgtgg gcgctaattt cccataatga ggaccaggag ccgacatcgc tccagcccgc
    47101 atccatcggc accacaacgg catctgccgt acgttccatg accgcgtaat ccaccgactc
    47161 ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcgagat ccggatcgac
    47221 ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatattttt tcagttcttc
    47281 gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt cgccgcttgc
    47341 cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca cttcaaaggc
    47401 caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat aaccggtttc
    47461 tggtagatcc ggcacaatgc cgaaggtcac cagcttgccc gcttcggcat atggcatggc
    47521 attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg ccgccaatac
    47581 cagcattaac gggtcgctct ccgggctatg acgttttgcc gccagcgccg ccagcgcaat
    47641 ggcaggtgcc gtgtttcgcc ctgccggttc gagaataatg ttctcggtaa gtttgttcag
    47701 ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca ccaccgggct
    47761 ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga gatcgccttt
    47821 caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc ggctaccgga
    47881 gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc ccgaatatca
    47941 tttataaatt gacgtaacac gttctcttta tcgagtgtgc gttcggcata ttcacgtgcc
    48001 accgtgttgt gtttgggcag caggagcgcc tgacggatcc ccgccaccag cgcctcgacc
    48061 gattccggtt ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg ccccagttct
    48121 gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt ggtcagtttc
    48181 gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca atcgcccatc
    48241 ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat gttgcgcagt
    48301 ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc gacaatggca
    48361 aaaatcagcg gttcatcgcg cagacgatcg gcagcttcaa taacgttttc cagcccctgc
    48421 ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag gtcaagctgg
    48481 ttacgaaggg cgtcaacatc ggcatctgca acatgctgaa aacgggcaat ttccgaccag
    48541 ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatggcttt attcatcatc
    48601 gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc gaacgccgtt
    48661 gccagctgtg ccactttgct gcctttgcct tttccggcaa ggcccagccc cagcatggcg
    48721 tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagaaagttt cgccagcagg
    48781 cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc cggcttccag
    48841 cgacgttgcg ccatcagagg aaagaaactg ctgacggcaa aactgccgag atgtaacagg
    48901 cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac cgtggcggcc
    48961 ccctcttctc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca ctgcgggtag
    49021 taaggcggtg cggtaataac ccgcacctca tgaccttgtg ccgccagcca ttccaccatc
    49081 tcgccggtgt atttgccgat accggttaac tccggcgagt agttaatgcc gtagaccagt
    49141 attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg cggctgttag
    49201 cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa tcgtcatgct
    49261 gctcatccgg cagtatcagc tcttcttcag cgactctgaa gcgaaaaccg agcaccacat
    49321 agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc cagacaccgt
    49381 aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc agccgctcga
    49441 atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac cagtaaccct
    49501 gcgccgggcg gttggttctt ttgccaagca gaaactcacc gcgactgttc tcgacaataa
    49561 agtcgagaga gacaagcggg gtggagcgca ctaccgtggc aaagtcttcc tgacgtaaaa
    49621 acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag tgctggcaag
    49681 ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg tcacatccag
    49741 cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac ctttgtaacc
    49801 caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc ccgtgccgac
    49861 gttaatgtgc gacagcatcg gctgggtgtt ctccagccag acttcgtgcg ccagctccat
    49921 gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg aattcacgca tcggtgtacc
    49981 gctgccccat accaccacgt ccggtgcatt ctgtgccgtc gcctcgtgga agcgacgcag
    50041 caatgcaggg atcacatgcg aattactcgg gtggaagttg tcatgcgggc catacaggtt
    50101 ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt cgcacagttt
    50161 gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg tgccctgcaa
    50221 taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg acgatccaag
    50281 aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc tctcaatcat
    50341 catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc cgcccacttt
    50401 cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat gcaccgcgcg
    50461 gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat caccgcgctg
    50521 ttcgagctgc cgcctgatgg cagaaccgac catcccacga tggccagcga taaaaatgcg
    50581 ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg agatttcagc
    50641 agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac catctctctg
    50701 agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc gccgagcagc
    50761 gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac atcacccggt
    50821 ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc ttcaacaccc
    50881 gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac gaactgacgc
    50941 acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg ttgcagcatc
    51001 atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga atccatattg
    51061 ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg ggtgattttg
    51121 cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat tccgttacaa
    51181 gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta cagtttggcg
    51241 accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat ttcctgcacc
    51301 agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc cagaccgagg
    51361 aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc ggtatattct
    51421 ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac ttcatccggc
    51481 tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc ataatgcaga
    51541 tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg ctcggtgttg
    51601 aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag cagaaactct
    51661 gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt tgacatgtct
    51721 tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca ccactgcggg
    51781 attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc gtgcacccac
    51841 gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg ccagccagca
    51901 tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg catggtcgtg
    51961 gctaccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa tggttatttc
    52021 accgagggta tataaattga cgtcatcgcc gacccacgcg taatcaccta aggttaattt
    52081 ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta tttttgctcc
    52141 gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc aggcaaatat
    52201 tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc ggaacccttt
    52261 cggcaccgag aaaccgctta aatcttgcat tggttttcct tatattcaga ctttgttata
    52321 taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt cagcccaaaa
    52381 gccaggcaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca attccatatt
    52441 attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga gttttttaaa
    52501 tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa ctttatattc
    52561 caggtcataa cgccattttt tcaagccgga tacagggaaa aatatcgcct gatgactggc
    52621 gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt tatgcccgtc
    52681 gccaaaatcc agcagcgcat cgccggtgat catcatgtta tctttttgca tttttaactt
    52741 acggacaaaa tttgcggcat cctgatgaaa aatatcgccc gaattgagaa acaacgcgaa
    52801 cttgccttgt gccatcgtaa tgcctttgtt catggcgtcg tagataccgt tatctggctc
    52861 gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac gggtgccgtc
    52921 attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct gcgccagatg
    52981 cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga cagtgattat
    53041 gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc aaaataaatg
    53101 gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa aacagcgata
    53161 ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa gcgttgatca
    53221 tcatttttat cactttcccc atgtaccaca gcgataaaaa cactgcgaac caggaaaaat
    53281 aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca ccgttaaata
    53341 ttccgaatga tgcgacgtat tcataaagtg agccaaatct gactacaccg tcaatatggg
    53401 tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat gacgatcctt
    53461 ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc acaccaacca
    53521 ggaatacagc cagagaaatc aatgccaacg gtaacttttt cttaatcgcc tctttattca
    53581 gatattgaaa tgcccactcc agcaaataaa acaggataaa agtcataacc cctgaaaacg
    53641 atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt ttgataccaa
    53701 actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc ggttcgaaat
    53761 aaagtgctgt cgtgcgcttg ccgccgaatt taatgaaatt cagtacatag ctgttgctgt
    53821 aaatcagata tttcgaaatc gactccataa tactgctgcc accggtgagg ataatttgcg
    53881 ccatctccac cgccgccagc gccaccacca gcccgatcac cagataaaag aaacgtaata
    53941 tcttgcgatg gttgtgtggc gaaatcgttt taaagcgaat actccacacc atgccaataa
    54001 tgatcacaat atagacaaac agcatggttg aagtgacgta tttactggca tccagcgact
    54061 gaccaaacag atagttaaac gccgtgagtc ccgcaccaat ccctaaggca atcatcaatt
    54121 ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa gtgacgatgg
    54181 tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg tagatcagcg
    54241 gcagcagcag gtagctacag attctgatag aagttgacat actcctccag catctgttgt
    54301 ccactgtagg tggcgcggct gcgttggctg aactcagcca gcgtggtacc aaatatcgcc
    54361 tgcgcgattt ccggtttgct taactgcacc agttgcagca cgtcttcttc gtcgacggtt
    54421 ttaccgccgg atttttgcaa cacttcccgt gccgcgtcgc tatgggtggc aatcaccggc
    54481 acgccaattg ataacgcctc acacaaaatc agcgggtagt tatcgacgcg agaactgaat
    54541 accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt ttcaaagccg
    54601 tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg cagttcgatt
    54661 ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagtcttgcc gtcgtaacgc
    54721 aagtcatgcg ccaccaccgc gattttcggc ttgccctggg tttcgcgcac cggaggcaag
    54781 tccgccagaa tcgcttcggt tgccatatca ataccattat tgataatccg gcaacgccct
    54841 ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat aaactgacag
    54901 cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagctg gtgtgcgcga
    54961 tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc cgttttccag
    55021 ccttcgcaac cgtcggtaaa ggcgcagcgt ccggtaacac tccagtggtc gtgcagcgtc
    55081 cagaccagag tgacgtccgg tttatggttt ttcacttttt cgcaaaagcg caccacgctc
    55141 ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg acccggtgtg
    55201 cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt aaacagacga
    55261 aacagggcaa tattcgccat cgcggtcatc cgcggcgtat gtttgatgac ctgcggatag
    55321 ttctgatggc tgacgctctc tttgccgcct ttgccgtaac cgtagacaaa atgtgacgcc
    55381 agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc cccgccttcc
    55441 gccagtcgca cattaaattg cagaatattc atttaattac cttcactcgc gctttttctc
    55501 ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca cccacggtga
    55561 cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt tcgacgccgt
    55621 tgccaatgtg tggacatgcc atgttatcgg caccacgatt gccgatagtg acgccgtggc
    55681 gaatggtgaa atcatccccc gctaccacgt ttttattgat cactacggcg taaccgtgat
    55741 ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg aaaaagcatt
    55801 cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg aggacgttct
    55861 ttttgcgcca caccgaacaa aaatgagcaa cacgataggc aagaaccatg cagcacgggc
    55921 gtaaactcca gctgttggca cgcagatctt ccagcatctt tagcgccccc gaataccatc
    55981 agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc aggtcatacg
    56041 tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgcttgcac ggtcaaattt
    56101 atctttgtgc tttcggtaaa agtggaagta cccggagaat tttttcggtg acgaggtgat
    56161 ctgcatttca ccgtgattga tatgcaggat ctgcgtcgct tcttccactt tccacggttc
    56221 gccgtactcc accaccatcc gcaggaagat gtcgtaatcc tgtgctgctt tcagttcggt
    56281 atcgaacaaa cactctttga aacgccatgc ccaagtaaag acctgattgc caatgatatt
    56341 gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta agcttgccgg
    56401 ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg cgtgagtgac
    56461 cagttgctgt ttatgggcga ggaagacgct cagacggttg ggtgtccatt cgtcatcgtc
    56521 atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac gtaccgcgca
    56581 cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga gggcagtgac
    56641 gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca tctcccagtt
    56701 gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt ggcggttcca
    56761 ggtcggcata tagattgaaa tcagcggatt ctctttcata ggttgttctc ccggcttatg
    56821 agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg gcggtgcgaa
    56881 tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc ataatcctga
    56941 tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg aataccgttt
    57001 tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc atagcgcgcc
    57061 accattaacg tggttccagc gtgacgcccg acaattgctg catcggtcac tgccagaatc
    57121 ggcggcgtat caatcaacac caggtcgtag tttttactcg cccagctcac caattcggca
    57181 aagcgttcgc tcatcaacag ttcagaagga tttggcggca cctgaccgcg cgggatcagg
    57241 tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg gccaatcaga
    57301 atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta gcctttgcgc
    57361 atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc cgccaggttg
    57421 gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa cacattgttc
    57481 tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc ttcaatcgcc
    57541 agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt aatccctttg
    57601 atggttttga cgctatcgcg cgctttctgc cattccgaca gcgggatgct ggcatagacg
    57661 ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa caacgagcgc
    57721 agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag gataatcagt
    57781 cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat gcgcacatcg
    57841 ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag ttgcatatag
    57901 acctgctgac cagactcgac atcgcgggtc aggcgcacaa tctcctgctg ggttttcggc
    57961 atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg acgtttctcc
    58021 agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc cgcctcttta
    58081 aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac cgctttcgct
    58141 tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt gttttcggca
    58201 acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag gcttttcgac
    58261 gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat gctgttaaga
    58321 atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac gcctgcgtct
    58381 ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc caacgtggag
    58441 tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac catcagcgtg
    58501 acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc gccatcactg
    58561 ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa acacctggcc cgccatctct
    58621 ttcgggcggt taaaggtagt cactttcacc gtctcgttct ggcgtcccat cagtcgctcc
    58681 cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg caatatcgag gtcgagatcg
    58741 tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc gtccgatgca
    58801 ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta acgaattgcc gctgttttgc
    58861 tcgatttgta ccagtgcgtc ggcactataa atcggcgttg cgaagaaggt gtaaaccacg
    58921 gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc aatgacggtg
    58981 ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc atgttgtttt
    59041 actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac tggcgggcag
    59101 accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga tacggatcgg
    59161 ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc atctcaggtg
    59221 ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg tcatagttgc
    59281 gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt tgatgttctg
    59341 cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg agtccagcgg
    59401 actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccgtc ggggaacggc
    59461 aaatattgcc gacacagaca actaatatgt tgttaaacat gacgattacc agttatgaat
    59521 gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcaaca actgattgat
    59581 cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat aaggttgcag
    59641 gcggaattct gtcgccatca ccagcgacgt ggcatcggac atatccagct ggtagatatt
    59701 ggcaatcttg ccattacgcc cgccctcgcc tttcagcgga cgaatgacaa agatgccgct
    59761 ggcgttggag gtggtcatgt cgataccttc agcattaccc agggcttcag tcagggtcat
    59821 gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca caaatacttt
    59881 cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc ggttctgatt
    59941 gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac cattgtgtgt
    60001 tagcactacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt tgatggcgtc
    60061 gagaatagtc agtggcacgt tggtgatcgc ctgttgaccg gatttattca cctgaccgga
    60121 gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg ggtcagcgat
    60181 gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt tcccgactac
    60241 gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc agttgccggt
    60301 gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat cccagacggt
    60361 gacattcaga acgtcgcccg gcccgacgcg atactggtaa ttcgcgatct cgctttccag
    60421 cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca ggcgcggggt
    60481 cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct gtttgatgac
    60541 gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc ttatcaaggt
    60601 tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt atttatcact
    60661 ttggcagagt aattatcctg tgcactatta atagcaatat cgccatgcac atttaccttg
    60721 cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt tcatccgcag
    60781 gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta ccgcccctgg
    60841 ctttttagcc accaatacac tgatttagct taatttttca aaccctctct gcatacaatc
    60901 gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac attatggtaa
    60961 ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga atagcaggca
    61021 aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat tcaatatggt
    61081 ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg cgatatttct
    61141 taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt attaccctca
    61201 aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta tccatgatcg
    61261 aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat ggattgccga
    61321 tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc tcggcattga
    61381 taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaggcagc gcgaccgcgc
    61441 acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg cgtcaatctc
    61501 ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct ttagcgcccg
    61561 cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga tggagctgaa
    61621 cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg cgaagttctg
    61681 gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact cagtgattac
    61741 cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta tcgctatcag
    61801 cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc cgacgatcgt
    61861 tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag aaggtttcgg
    61921 cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga tgatcgaggc
    61981 gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc agacgctgcg
    62041 ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag atgcggaact
    62101 ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct ttaatccgca
    62161 ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca gcagcattat
    62221 gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag aaatccgcca
    62281 gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg acgcagaaga
    62341 tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg gcgaaccgct
    62401 caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc cgttgttacc
    62461 tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg atgagtttgg
    62521 ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgtcg gtaacttacc
    62581 gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg acggttcctg
    62641 gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc tgccgctgga
    62701 tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac agcgtattcc
    62761 aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc aggtggaaag
    62821 ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt tggagtacga
    62881 ggtgtgatgg tatcggtcat tttacccaat agccgaaagg aataagatga gggaaccgcg
    62941 ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt gagccaccct
    63001 ggcacgttca ccggcttgag cgttccatag tagcgaggaa catcaagtga acggaacagc
    63061 cttcgatctt acatattcag aatataatga tgccgccacg aacgcctgat gcgacgcttt
    63121 acgcgtctta tcaggcctac aggactggtc acctggtaac ctacatcttc tccagcaact
    63181 tcttcacatc cttgccattg cgggaatctt tattccgctc ggcccagtca ttcaggcgac
    63241 gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg ctgtaattga
    63301 gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttcagg aaatcaatca
    63361 gtttactttc cccgttccag ccacccacga cccgaatatc aaactgggta tcgcaggttt
    63421 gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat tgaccttgca
    63481 tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag cggtcaagcc
    63541 gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc tctaccgctt
    63601 gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt tcgacatgtg
    63661 cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg gagaaatcac
    63721 cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc agaatggtac
    63781 caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt gatgtcgcgt
    63841 ccagcgtgcc cggcagtgaa acctgtccac cgttaagttt gccctgcagt tgagtaattt
    63901 ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc attccgcgcc
    63961 agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt ccctgatagg
    64021 ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg ctctgcccct
    64081 gctgcgcggc accgttttca ccattcgcgg tttcattaag cgggatgagg ttgtcaagat
    64141 tcagttgcgg gaattgcagt ctcagctgcc attccggttt ctctgtcagc gtgacctgtg
    64201 cttgcccgct cagcgtactg tcattggcgg tcaagctgat ttggttaaat gaaaggcgtt
    64261 tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct tgttttggta
    64321 aatcggcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa tcatgcggat
    64381 aatcagaagc atccaccgta ccgttaaggg atattgtgag atcgcgctga tcgcgattaa
    64441 cccgcccgga gaactcaaat gagccacgat gttggggatc ttgttccatt tgcaggcgga
    64501 tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg ctgtccgcca
    64561 ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc ggcaaggtat
    64621 tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc ggcgtcagtt
    64681 ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc agtagtggta
    64741 aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt gcgccctggg
    64801 cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc caacgcagtg
    64861 gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc atatagtcgc
    64921 ggaaatcatt cggattcacc agcaacacta acgcagataa cccggccacc agcacgacca
    64981 ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc ctgaattagt
    65041 ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca tcttcacggc
    65101 ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca ccaattaaca
    65161 tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc acaatgcaac
    65221 cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc agacgcgcca
    65281 aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc gactccagcg
    65341 tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg tcgagaacga
    65401 tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga ccgctcagat
    65461 cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg cgtacatcca
    65521 ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa cggccttcat
    65581 caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct ttcgcgcatc
    65641 actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg gtacacaatt
    65701 tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat cgcgatgcgg
    65761 tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc aatgaattgc
    65821 aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat tgaacgccca
    65881 cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg ggtatcaacg
    65941 aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa aatgccttcg
    66001 agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt atgttcaaca
    66061 tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa atgctcaagc
    66121 agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg ttcttccatc
    66181 gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat gtgttcatca
    66241 ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt gccggaagcc
    66301 gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat atatttagcg
    66361 acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg caattatagg
    66421 gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt taattgttga
    66481 caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa aaatgttata
    66541 cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca tataaaatgt
    66601 aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc ttgcggataa
    66661 agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc cgctgcttca
    66721 cctggtcagt ttaggtttag tctcgtttat ctttaccctt ttctcgcttg agctttcgca
    66781 gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg tggcgtttta
    66841 tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg gaaatatcgc
    66901 cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga ccatcaatat
    66961 tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt ataacccctt
    67021 gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc cgcctctgtt
    67081 ggggggtgtt ctggttgtcc tgctgacgcc cggagacgat cctctcaggg catttttgat
    67141 atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat tgttatttaa
    67201 accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc tgctcacgtt
    67261 agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc cttttacttt
    67321 cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag cctttttgat
    67381 cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct ccctgcttgc
    67441 tacgccgcgt acgtacctga tgagccatat gccgtggcta ccgtttttgc tgatcctgct
    67501 gcccgccaac atcatgacca tggtgatgta tgcctttcgt gcggaacgca aacacatttc
    67561 cgaaagcgaa acccgctttc ggaacgcgat ggaatattcc gctatcggta tggcgttagt
    67621 gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc tcgggtacag
    67681 tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg atctcaataa
    67741 agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt caatggaaaa
    67801 acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct cactggtgcg
    67861 ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta acgagctaaa
    67921 acgcaccgaa caggtcaatc agcaactgat ggagcgcatc acgctggcta acgaagcggg
    67981 cgggattggc atctgggagt gggagttgaa gccgaatgtt tttagctggg ataagcggat
    68041 gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt ggtacgagtg
    68101 cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt tgcaatcacg
    68161 ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc atatccgcgc
    68221 cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgtctcctcg gcatcaatat
    68281 ggatatgacc gaagtgaaac agcttaacga ggcattgttt caggaaaaag agcgcctgca
    68341 cattacgctt gattccatcg gtgaagccgt ggtctgtatt gatatggcga tgaaaattac
    68401 ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag cgttaggtgt
    68461 tccgctcctg acggtgttgc atattacttt tggcgacaac ggaccattaa tggagaacat
    68521 ttacagtgcc gacacctcac gttccgcaat cgaacaagat gtggtgttgc actgtcggag
    68581 cggcggcagt tacgacgtgc attacagtat tacgccgtta agtactctgg acggcagcaa
    68641 tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc tgcgccagct
    68701 gagctacagc gcctcccatg atgcactgac gcatctcgcc aaccgcgcca gttttgagaa
    68761 acaactgcgt atcctgctgc aaacggtaaa cagtacgcat cagcgacatg ccctggtgtt
    68821 tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg cgggcgacgc
    68881 tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgctccagtg acgtgctggc
    68941 gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgttg aaagcgctcg
    69001 ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatat gggaaggccg
    69061 ggtacatcgg gtaggtgcca gcgccgggat taccttgatc gatgacaaca atcatcaggc
    69121 ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg gtggacgggg
    69181 ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg cggcgatgtc
    69241 gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg cccacggtgt
    69301 cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta agctctggag
    69361 ttgcgaaggc gaaattattg atgaacaaac atttcgtcgt agcttcagcg atccggcact
    69421 tagccatgct cttgaccgac gggtattcca cgaatttttc cagcaggccg ccaaagcggt
    69481 tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga gtagcgccac
    69541 gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac ggttattaca
    69601 tctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa aactgcagct
    69661 ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct tcaactcgct
    69721 gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca acgtgcaggg
    69781 taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc gactcgggat
    69841 gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt ctggcatcgg
    69901 cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt tgctggtgaa
    69961 tagcagttat ttcgcgatta actgagagag cgtttgccca ttgcctgatg cgacgctaac
    70021 gcgtcttatc atgcctacaa atcgctcatt ccccagaccg gataaggcgc tcgcaccgca
    70081 tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca gatatgcaac
    70141 agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg tgccggtgtc
    70201 attcccggaa atcgccgttt aatcagataa tcatccggca gaaaaacatc tttcgcctgc
    70261 cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa agtttgcagc
    70321 gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc ctccagcgcc
    70381 gcatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa cgcttttaat
    70441 gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata ctccggaaaa
    70501 tcatccagcc gttcgccata aagctgtacc actttggcag tcaattttgc cgccatcgcc
    70561 acgctcacca gttggcctaa aatcgcccgc acaccctgct caaaagcatc aatacagccg
    70621 ggtaaacgca atccgggccg ccccgcgcct aactttccca acgcaccgtt aacaatctgc
    70681 gggttacatt gcagatcaag caggcggctc attttcgcca gacactctgc ggcaacaggt
    70741 tctaaacctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg aatagcagtc
    70801 actacgccgc gatattcgcc caccgccaga ctacgggcat aataattgtc cgcgaccgtt
    70861 tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca gtcatacggc
    70921 ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag cataaacgtt
    70981 attcagacgt tacgctttgc tttcatattc cggttgtcgc gacagcaaca tttcgctaaa
    71041 gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt tgattacggt
    71101 acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact gaaaatggaa
    71161 aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaatc ggtaagcgaa
    71221 tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc gctgttacgt
    71281 cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag cgtgcagttc
    71341 ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt tgtgaaatca
    71401 ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct gtttgaggat
    71461 ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct gccagaagcg
    71521 attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg cgatgaagca
    71581 aacgcccagg cgcaagagat tctggaacgc gcggcgaagc gtgccggatt cagggacgtg
    71641 gtattccagt acgagccgat cgcggctggg ctggattacg aagccacctt gcaggaagaa
    71701 aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt gctgctgatg
    71761 gggccgcagt ggcgttcgcg tctcgatcgt gaagccagtc tgctgggtca cagtggttgt
    71821 cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat gccactgctg
    71881 ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg gtggaatgcg
    71941 gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa cggtcgtctg
    72001 cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt acagaaagtc
    72061 tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa aattgctctt
    72121 tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact ggctacgctg
    72181 attagccagc gagggctgga aagcgccctc agccagccac tggcgcggat tctggaacag
    72241 gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct gaccggcggt
    72301 agcgcccgtt ctccgcagat taaaaaagcg ctggcagaac agttgccggg cattccgatt
    72361 gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc ggaagtggtg
    72421 tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct gagccagttt
    72481 ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag catgaaggcg
    72541 gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat gtcgtttggc
    72601 ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat tcggcctgaa
    72661 cacaaaacgg cgttcacagt tcgctttcca gtcgataacc gcctgggtca gatggtcgcc
    72721 aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt caataccaaa
    72781 agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa catcaataaa
    72841 aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa gtgctgccag
    72901 agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt cgagcatcgt
    72961 ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga ctaaatggtc
    73021 atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg ctttgtacgc
    73081 tttgcttgtc aaactgccaa taatccagat agccgccagc acaggaacca aaataatata
    73141 acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg cgatctcaag
    73201 cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag tcggttggaa
    73261 atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca ccatcgccca
    73321 gacttttgcc agcacaaaat ccccgccggt ggtaatgacc tcttcgccga gatcaataaa
    73381 atagataaca ccttgattat ccagcgcaca ccatgggcag tcagccaaat gaccgggata
    73441 aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat ccagtgctga
    73501 tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtaa acgcctgctg
    73561 aaacatggct tcaacatcac tcggtaacat cgatagcgga atcgatcgtg gcggcggttt
    73621 taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa tatccgtctc
    73681 cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc ggccaccaaa
    73741 caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg tgcgttcaaa
    73801 gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca cgccgacttc
    73861 gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa tcaacaccac
    73921 tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga cgtgcccgtg
    73981 ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta gaaaatccca
    74041 cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat gaatcggttc
    74101 tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc caccgtgaag
    74161 cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg cagccataaa
    74221 ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc
    74281 gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt cacggcctgg
    74341 cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca cagcgccagt
    74401 gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa aaactgcttt
    74461 aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc attgaaaaac
    74521 ggggtaaaga aaggcacatg aggagaatta tccagcatat ttaacgccag ccgctgaata
    74581 ccatccgtaa atgctgcaac tttatgcgca cgcccagtgc tggtgaaagt ttccagtctg
    74641 gaaacggcat cttcgtcggt aatgaagtgc gtcatgttgg catattcacc caccatcggc
    74701 gtgagaggca attgcagacc gtgacccaaa tctaccacca cgccgccatc gccaatctgc
    74761 ataatcaagg tgccgtcagg cgatgaaatc aatcccagaa acgtgcaggc aaaatcgcga
    74821 actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatagt cagtaccata
    74881 tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg caccataaac
    74941 gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac gctacctgcg
    75001 ccatcagcaa ggaacatcac cagtaatggt tgctggtcgt ttaaccagac aacctgcatc
    75061 tggcaggcat cctgacaagg caaatcagca ctaatgtgcg atgttccaac agcggatgca
    75121 taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc acaacttccg
    75181 ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta aacagttcac
    75241 ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc agagtcttca
    75301 tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct tccccctgaa
    75361 acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg atcaggaaaa
    75421 tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc tcttcaacca
    75481 tatccagggc tttggtaatg gcggccccca taggcgtatc cccctgggca aacaggatgg
    75541 gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg ccgaacgtca
    75601 caatgccgag ttccactctt tttaaagcca gcggatcagc aagcagttca tcacgaaagg
    75661 taaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca gaaacatcca
    75721 gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg gtggcaaacg
    75781 tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg gtatcatacc
    75841 gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc cgtcggcgca
    75901 gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact aagggcggag
    75961 tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg gtgttcttag
    76021 taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt catcataacc
    76081 ctttccttat taaaagccct cttctccggg agagggtttc ccgtttcagc gtcccgctga
    76141 aatcgtcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg tgaacaaaat
    76201 agtttcatct gtgattattg cgtaacgact cgcaaagaat atttgctggc aggatcgcag
    76261 actacaaagc ctgcggattg acaatcttat cgtgaaggca taatttcagg agtgagggta
    76321 gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg actaagagca
    76381 ccacgatgat gagtagcttc atcatgaccc tttccttatt aaggcccctt cctcgggagg
    76441 ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg ccctgtatgc
    76501 agtctatcgc tgacttgcgg ataaaatagt ttccagtgtg attattgcgt aacgtctcgc
    76561 aaagaatatt tgctggcagg atcgcagact gcaaagcctg cagattgaca atcttatcgt
    76621 gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg tagccttagt
    76681 aagctggcca gctaatgact aagagcacca cgatgatgag tagcttcatc atgacccttt
    76741 ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc gctgaaatcg
    76801 gcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat aaaatagttt
    76861 cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg attcccgaca
    76921 gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt atcattccgc
    76981 gaaacgtttc aggaagagaa actcttaacg atgaaaggca gttataaatc ccgttgggta
    77041 atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg caatgactcc
    77101 cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt
    77161 ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga acaggcagtt
    77221 ccgcgttacc tcaccgggct tggcaccatt atcgccgcta atactgttac ggtgcgcagc
    77281 cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt caaagcaggc
    77341 gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca aacccagggc
    77401 caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc gcgttatcag
    77461 caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca ggcgctggtc
    77521 agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc gcagttgcaa
    77581 ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa gcaggttgat
    77641 gttggtaacc aaatctccag tggtgatacc accggaattg tggtgatcac ccagacgcat
    77701 cctatcgatt tagtctttac cctgccggaa agcgatatcg ctactgtagt gcaggcgcaa
    77761 aaagccggaa aaccgctggt ggtagaagcc tgggatcgca ccaattcgaa gaaattaagt
    77821 gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac gattaaagtg
    77881 aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt taacgcgcgc
    77941 atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cagccgccct gcaaatgggc
    78001 aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa acatctggtg
    78061 acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc tgcgggcgat
    78121 cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga agtggtggaa
    78181 gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga
    78241 gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg cgcctgttta
    78301 ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc gggattatcg
    78361 gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc attcaggtgg
    78421 tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc gcgccgctcg
    78481 aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt tccggcggtg
    78541 cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc gagcaggaag
    78601 tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct aacccgccgg
    78661 tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc acctcaaccg
    78721 ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc
    78781 agatttccgg cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct gttcgcgtca
    78841 aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca
    78901 ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc cgtgcagtca
    78961 cgctttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagcta atcatcgcct
    79021 accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa ggtgcagaaa
    79081 acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat gttcagcgcc
    79141 agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg ccacagctca
    79201 ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc accaatatcc
    79261 gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg gtagtcatga
    79321 ttatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt gctgtaccgc
    79381 tgtcgttaat cggcactttc gcggttatgg tgtttctcga tttttcaatc aataacctga
    79441 cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc gtggtgatcg
    79501 aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg ctcaagggcg
    79561 caggtgaaat cggctttacc attatctcgc tgaccttctc actgattgcg gtgttgatcc
    79621 cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct attaccctgg
    79681 cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg atgtgcgcgc
    79741 ggatgctcag ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa
    79801 tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaagtgctg aatcatccgt
    79861 ggctgacctt aagcgtggca ctcagcacgc tgctgcttag cgtgctgctg tgggtgttca
    79921 ttccgaaagg tttcttcccg gtacaggaca atggcattat tcagggcact ttgcaggcac
    79981 cgcaatccag ctcctttacc aatatggccc agcgacaacg ccaggtcgcg gacgtgattt
    80041 tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc actaacccgt
    80101 cgctgaatag tgcacgttta caaatcaacc tcaaaccgtt ggatgaacgt gatgatcggg
    80161 tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa agtgccgggc gtcgatctct
    80221 tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc cagtaccagt
    80281 ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa
    80341 aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac caagggctgg
    80401 tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc atggcggatg
    80461 tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt tatactcagg
    80521 ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc ctcgcggcgc
    80581 tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc tcaattgcca
    80641 aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc ccggtaacga
    80701 ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag gagattatgg
    80761 acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcaattccag ggcagcaccc
    80821 tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg gtggtggcga
    80881 tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc attctctcga
    80941 cgctacccac cgcaggggtc ggcgcgctgc tggcattaat gattgccggt agcgaactgg
    81001 atgtgattgc gattatcggc attattttgc tgatcggtat tgtgaagaag aacgccatca
    81061 tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg cgcgatgcaa
    81121 tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg gcggctctgc
    81181 ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt cgtccgttag
    81241 gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc accacgccgg
    81301 tgatttatct gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt gctcgccatg
    81361 aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg gcgacgattt
    81421 tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg ccggtcgccc
    81481 cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcac
    81541 cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcgcttggg cgcattgccg
    81601 gggtcagcga aatgacctcc agcagttcgc tcggcagcac acgtattatt ttgcagtttg
    81661 attttgaccg ggacatcaac ggcgcagcgc gtgatgtgca ggcggcgatc aacgctgcac
    81721 aaagtttgct gcccagtggg atgcccagcc gtccgaccta tcgcaaagcg aacccgtcgg
    81781 atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt gaactgtacg
    81841 atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtt ggtgatgtcg
    81901 atgtcggagg cagctcactg cccgccgtac gcgtcgggct gaatccgcag gcgctgttta
    81961 atcagggcgt gtcgctggac gacgtacgca ccgccatcag caatgccaac gtgcgtaaac
    82021 cgcagggcgc gctggaagat ggcactcacc gctggcagat ccagaccaat gatgagctaa
    82081 aaaccgccgc tgaatatcag ccgttgatta ttcactacaa caacggcggc gcggttcgtc
    82141 tgggcgatgt ggcgacggtg accgactcag tgcaggatgt gcgcaacgcc gggatgacca
    82201 acgccaaacc ggctatttta ctgatgatcc gcaaactgcc ggaagccaat attatccaga
    82261 cggttgacag catccgggca aaattaccgg agttgcagga aaccattccg gcggcgattg
    82321 atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggaa gaagtcgagc
    82381 aaacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttattc ctgcgctcgg
    82441 gtcgcgccac tattattccc gccgtttcgg tgccggtttc gctgattggt acgtttgcgg
    82501 cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc accatcgcta
    82561 ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcacgt catctggaag
    82621 cgggaatgaa accgttgcaa gccgcactgc aaggtactcg cgaagtcggt tttacggtgc
    82681 tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg ggcggattgc
    82741 cgggccgact gttacgcgag tttgccgtga cgctttctgt cgccattggt atttcgttgc
    82801 tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc agcaagccgc
    82861 gcgagcaaaa gcgactgcgt ggttttggtc gcatgttggt agccctgcaa caaggctacg
    82921 gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg ctgcttggca
    82981 ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc ccggagcagg
    83041 acactggcgt gttgatgggc gggattcagg cggatcagag tatttcgttt caggcgatgc
    83101 gcggtaagtt gcaggatttc atgaaaatta tccgtgacga tccggcagtg gataatgtca
    83161 ccggctttac aggcggttcg cgagtgaaca gcgggatgat gtttatcacc ctcaagccac
    83221 gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta aaactggcga
    83281 aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt ggtgggcgtc
    83341 agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca ctgcgcgaat
    83401 gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac gtgaactccg
    83461 atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc atggcacggc
    83521 tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt cagcggcaaa
    83581 tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg gatccgcgct
    83641 atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa ggcaaagcga
    83701 tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc tccgctgtcg gtgaatcatc
    83761 agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa tcgctctcgg
    83821 acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg acggtgcgcg
    83881 gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag gtgatcctga
    83941 ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag agttacgtac
    84001 acccactgac gattctctcc accctgctct cggcgggcgt tggtgcgctg ctggcgctgg
    84061 agctgttcaa tgccccgttc agcctaatag ccctgatagg gatcatgcta ttaatcggca
    84121 tcgtaaagaa aaacgccatt atgatggtcg attttgcgct cgaagcccaa cggcacggta
    84181 acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc ccgattatga
    84241 tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc ggtgacggct
    84301 cggagctgcg gcaccccctg gggatcacca ttgtcggtgg actggtaatg agccagcttc
    84361 tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg ctgcgttttt
    84421 cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga acttcccgac agcacccgct
    84481 gacaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc accatcgtta
    84541 acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg
    84601 tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc tggctggcgg
    84661 acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact ctcggttcac
    84721 tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg ttacagggcg
    84781 ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc gtaccgcgcg
    84841 agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt ccgctgctcg
    84901 gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc tttttgatca
    84961 acattccggt ggggattatc ggtgcgatcg ccacattgct gttaatgccg aactacacca
    85021 tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc atggctgtat
    85081 taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg attgctggcc
    85141 tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga aataacaacc
    85201 gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc ctggcgggga
    85261 gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt ttcctgcaaa
    85321 ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg gtacttggca
    85381 gcatgggaat gaagcgaatt gtggttcagg tggtgaatcg ctttggttat cgtcgggtac
    85441 tggtagcgac cacgctgggt ctgtcgctga tcaccctgtt gtttatgact accgctctgt
    85501 tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc aactcgaccc
    85561 gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg gcgagcagcg
    85621 gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc accatcgccg
    85681 ggctgttgct gggacttttt ggttcgcagc atgtcagcgt cgacagcggc accacacaaa
    85741 ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg gcgttcatct
    85801 ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg
    85861 cgcaatgaag ttctggcgac ctggtattac cggcaaactg tttctggcga ttttcgccac
    85921 ctgcattgtt ttgctgatca gtatgcactg ggcggtgcgt atcagttttg agcgtggctt
    85981 tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg atgcgcttgg
    86041 cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc gctttgtctt
    86101 tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc cgggtatgcc
    86161 accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag tgctggttgg
    86221 tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca acggtgcgga
    86281 agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg atatcaattt
    86341 cgataaacaa cagcggcaaa ccagctggtt gattgtcgcc ctggcaacgt tactcgcggc
    86401 acttgccact tttctgctgg cgcgcggttt actggcaccg gtaaaacgac ttgtcgatgg
    86461 cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca gtgaagatga
    86521 actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga aaaaccagca
    86581 aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat tagcggtgct
    86641 gcgcggtgaa ctggaagcta ttcaggatgg cgtgcgcaaa ttcacgccgg agacggtggc
    86701 gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc atcagttgtc
    86761 gatgtctgat gaaggcgccc tcgcctatca aaaagcaccg gtagatttga tcccactgct
    86821 ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga aactgcaatt
    86881 ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc agttattcaa
    86941 taatttactt gaaaacagcc tgcgctacac tgacagcggc ggcagcctga aaatctctgc
    87001 cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag gtgtcagtga
    87061 cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc gcaaccgcgc
    87121 cagcggtggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag cacataatgg
    87181 ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag agttaccgct
    87241 ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa cacaccacgt
    87301 attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta tctgcgtgct
    87361 gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta tgtgcgccag
    87421 acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg cttgacgctg
    87481 tgccgggaaa ttcgtcgttt ttctgacatt cctatcgtga tggtgacggc aaaaatcgaa
    87541 gagatcgacc gcctgctcgg actggagatt ggcgcagatg actacatctg caagccgtac
    87601 agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa accgcagcgc
    87661 gaattgcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg ttttcaggct
    87721 tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgtt gaaaacgctc
    87781 tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct ttatgacgac
    87841 taccgcgtag taaccgaccg caccattgac agccacatta aaaacctgcg ccgcaagctg
    87901 gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg ttaccgctgg
    87961 gaagccgacg cctgccgcat cgtttagttt tagcgacatg actttgttag ccggagatgg
    88021 cgttcagacc agatccggca acattatccc acgcatggtc agcaaactg
//
LOCUS       DAQSWQ010000002        80888 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.002, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000002 DAQSWQ010000000
VERSION     DAQSWQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 80888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.002"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="R8G18_000089"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="R8G18_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEE9793902.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="R8G18_000090"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="R8G18_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEE9793903.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="R8G18_000091"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="R8G18_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HEE9793904.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="R8G18_000092"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="R8G18_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEE9793905.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="R8G18_000093"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="R8G18_000093"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEE9793906.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="R8G18_000094"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="R8G18_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HEE9793907.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="R8G18_000095"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="R8G18_000095"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEE9793908.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="R8G18_000096"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="R8G18_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEE9793909.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="R8G18_000097"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="R8G18_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HEE9793910.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="R8G18_000098"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="R8G18_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HEE9793911.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="R8G18_000099"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="R8G18_000099"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEE9793912.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="R8G18_000100"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="R8G18_000100"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEE9793913.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="R8G18_000101"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="R8G18_000101"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HEE9793914.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="R8G18_000102"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="R8G18_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HEE9793915.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="R8G18_000103"
     CDS             complement(14282..14581)
                     /locus_tag="R8G18_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HEE9793916.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="R8G18_000104"
     CDS             complement(14793..15233)
                     /locus_tag="R8G18_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEE9793917.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="R8G18_000105"
     CDS             15334..15933
                     /locus_tag="R8G18_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HEE9793918.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="R8G18_000106"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="R8G18_000106"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEE9793919.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="R8G18_000107"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="R8G18_000107"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HEE9793920.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="R8G18_000108"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="R8G18_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HEE9793921.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="R8G18_000109"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="R8G18_000109"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEE9793922.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="R8G18_000110"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="R8G18_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HEE9793923.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21325..21543)
                     /gene="ogrK"
                     /locus_tag="R8G18_000111"
     CDS             complement(21325..21543)
                     /gene="ogrK"
                     /locus_tag="R8G18_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HEE9793924.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21625..22788)
                     /locus_tag="R8G18_000112"
     CDS             complement(21625..22788)
                     /locus_tag="R8G18_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HEE9793925.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22788..23267)
                     /locus_tag="R8G18_000113"
     CDS             complement(22788..23267)
                     /locus_tag="R8G18_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9793926.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23282..25729)
                     /locus_tag="R8G18_000114"
     CDS             complement(23282..25729)
                     /locus_tag="R8G18_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEE9793927.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25722..25841)
                     /locus_tag="R8G18_000115"
     CDS             complement(25722..25841)
                     /locus_tag="R8G18_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HEE9793928.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            complement(25874..26149)
                     /locus_tag="R8G18_000116"
     CDS             complement(25874..26149)
                     /locus_tag="R8G18_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HEE9793929.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26206..26724)
                     /locus_tag="R8G18_000117"
     CDS             complement(26206..26724)
                     /locus_tag="R8G18_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HEE9793930.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26737..27927)
                     /locus_tag="R8G18_000118"
     CDS             complement(26737..27927)
                     /locus_tag="R8G18_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HEE9793931.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28338..29756
                     /locus_tag="R8G18_000119"
     CDS             28338..29756
                     /locus_tag="R8G18_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9793932.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29871..30398)
                     /locus_tag="R8G18_000120"
     CDS             complement(29871..30398)
                     /locus_tag="R8G18_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEE9793933.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30402..32720)
                     /locus_tag="R8G18_000121"
     CDS             complement(30402..32720)
                     /locus_tag="R8G18_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEE9793934.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32731..33261)
                     /locus_tag="R8G18_000122"
     CDS             complement(32731..33261)
                     /locus_tag="R8G18_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HEE9793935.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33254..34162)
                     /locus_tag="R8G18_000123"
     CDS             complement(33254..34162)
                     /locus_tag="R8G18_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HEE9793936.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34167..34514)
                     /locus_tag="R8G18_000124"
     CDS             complement(34167..34514)
                     /locus_tag="R8G18_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEE9793937.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34511..35146)
                     /locus_tag="R8G18_000125"
     CDS             complement(34511..35146)
                     /locus_tag="R8G18_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEE9793938.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35213..35665)
                     /locus_tag="R8G18_000126"
     CDS             complement(35213..35665)
                     /locus_tag="R8G18_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEE9793939.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35658..36125)
                     /locus_tag="R8G18_000127"
     CDS             complement(35658..36125)
                     /locus_tag="R8G18_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9793940.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36088..36246)
                     /locus_tag="R8G18_000128"
     CDS             complement(36088..36246)
                     /locus_tag="R8G18_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein"
                     /protein_id="HEE9793941.1"
                     /translation="MPGSDPQTNGDLSADIRQLENALARCASQVKMIKHCQDENDAQT
                     RQPAQGAD"
     gene            complement(36233..36658)
                     /locus_tag="R8G18_000129"
     CDS             complement(36233..36658)
                     /locus_tag="R8G18_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HEE9793942.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36646..37071)
                     /locus_tag="R8G18_000130"
     CDS             complement(36646..37071)
                     /locus_tag="R8G18_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HEE9793943.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37086..37583)
                     /locus_tag="R8G18_000131"
     CDS             complement(37086..37583)
                     /locus_tag="R8G18_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HEE9793944.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37583..37864)
                     /locus_tag="R8G18_000132"
     CDS             complement(37583..37864)
                     /locus_tag="R8G18_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEE9793945.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37868..38071)
                     /locus_tag="R8G18_000133"
     CDS             complement(37868..38071)
                     /locus_tag="R8G18_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HEE9793946.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38071..38580)
                     /locus_tag="R8G18_000134"
     CDS             complement(38071..38580)
                     /locus_tag="R8G18_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HEE9793947.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38680..39423)
                     /locus_tag="R8G18_000135"
     CDS             complement(38680..39423)
                     /locus_tag="R8G18_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HEE9793948.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39427..39645)
                     /locus_tag="R8G18_000136"
     CDS             complement(39427..39645)
                     /locus_tag="R8G18_000136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HEE9793949.1"
                     /translation="MLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVE
                     DYAAGCLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(39582..40499)
                     /locus_tag="R8G18_000137"
                     /pseudo
     CDS             complement(39582..40499)
                     /locus_tag="R8G18_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228319.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
     gene            complement(40558..41412)
                     /locus_tag="R8G18_000138"
     CDS             complement(40558..41412)
                     /locus_tag="R8G18_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HEE9793950.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41586..43358
                     /locus_tag="R8G18_000139"
     CDS             41586..43358
                     /locus_tag="R8G18_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HEE9793951.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43358..44392
                     /locus_tag="R8G18_000140"
     CDS             43358..44392
                     /locus_tag="R8G18_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEE9793952.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44764..45906
                     /locus_tag="R8G18_000141"
     CDS             44764..45906
                     /locus_tag="R8G18_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEE9793953.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            45908..46588
                     /locus_tag="R8G18_000142"
     CDS             45908..46588
                     /locus_tag="R8G18_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9793954.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNLIESTMREAICLIHETIYDRNVEFDKLRKNLRSEILKRLK
                     NESVNVESLVNHLTKGISCGISYGTFNKKKIFSGNIDREEIKEKSKIYGFSTHSDYTH
                     TKHGEKLATVKQHRNDLSHGNVSFAEIGKNVSYQDLENISLEVIAYLDAIVNNIEHYI
                     NNNEYLEQ"
     gene            complement(46618..47640)
                     /locus_tag="R8G18_000143"
     CDS             complement(46618..47640)
                     /locus_tag="R8G18_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEE9793955.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVYNDFYNSLLKLGYYVWASKIDCVEYGIPQNRS
                     RHVLLASKLGAIELVKRDDVILKTVRDAIGNLPPLEDGQTDPNDILHRASKLNPINKK
                     RIIHSVPGGTWKDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITTLCYGFGNGR
                     FGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPVELGKAIGQS
                     IKNHLE"
     gene            complement(47727..50024)
                     /locus_tag="R8G18_000144"
     CDS             complement(47727..50024)
                     /locus_tag="R8G18_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HEE9793956.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(50014..50289)
                     /locus_tag="R8G18_000145"
     CDS             complement(50014..50289)
                     /locus_tag="R8G18_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HEE9793957.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(50286..50510)
                     /locus_tag="R8G18_000146"
     CDS             complement(50286..50510)
                     /locus_tag="R8G18_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEE9793958.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(50513..50812)
                     /locus_tag="R8G18_000147"
     CDS             complement(50513..50812)
                     /locus_tag="R8G18_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HEE9793959.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(50812..51036)
                     /locus_tag="R8G18_000148"
     CDS             complement(50812..51036)
                     /locus_tag="R8G18_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HEE9793960.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(51100..51600)
                     /locus_tag="R8G18_000149"
     CDS             complement(51100..51600)
                     /locus_tag="R8G18_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HEE9793961.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(51770..52042)
                     /locus_tag="R8G18_000150"
     CDS             complement(51770..52042)
                     /locus_tag="R8G18_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9793962.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            52179..52472
                     /locus_tag="R8G18_000151"
     CDS             52179..52472
                     /locus_tag="R8G18_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEE9793963.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            52542..53522
                     /locus_tag="R8G18_000152"
     CDS             52542..53522
                     /locus_tag="R8G18_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEE9793964.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(53708..54208)
                     /gene="cpxP"
                     /locus_tag="R8G18_000153"
     CDS             complement(53708..54208)
                     /gene="cpxP"
                     /locus_tag="R8G18_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HEE9793965.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            54358..55056
                     /gene="cpxR"
                     /locus_tag="R8G18_000154"
     CDS             54358..55056
                     /gene="cpxR"
                     /locus_tag="R8G18_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HEE9793966.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            55053..56426
                     /gene="cpxA"
                     /locus_tag="R8G18_000155"
     CDS             55053..56426
                     /gene="cpxA"
                     /locus_tag="R8G18_000155"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HEE9793967.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(56532..57206)
                     /gene="yiiM"
                     /locus_tag="R8G18_000156"
     CDS             complement(56532..57206)
                     /gene="yiiM"
                     /locus_tag="R8G18_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HEE9793968.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(57355..58338)
                     /gene="kdgT"
                     /locus_tag="R8G18_000157"
     CDS             complement(57355..58338)
                     /gene="kdgT"
                     /locus_tag="R8G18_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HEE9793969.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(58598..59218)
                     /gene="sodA"
                     /locus_tag="R8G18_000158"
     CDS             complement(58598..59218)
                     /gene="sodA"
                     /locus_tag="R8G18_000158"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HEE9793970.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            59503..60537
                     /gene="rhaT"
                     /locus_tag="R8G18_000159"
     CDS             59503..60537
                     /gene="rhaT"
                     /locus_tag="R8G18_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEE9793971.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(60534..61400)
                     /gene="rhaR"
                     /locus_tag="R8G18_000160"
     CDS             complement(60534..61400)
                     /gene="rhaR"
                     /locus_tag="R8G18_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HEE9793972.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(61456..62292)
                     /gene="rhaS"
                     /locus_tag="R8G18_000161"
     CDS             complement(61456..62292)
                     /gene="rhaS"
                     /locus_tag="R8G18_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HEE9793973.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            62580..64049
                     /gene="rhaB"
                     /locus_tag="R8G18_000162"
     CDS             62580..64049
                     /gene="rhaB"
                     /locus_tag="R8G18_000162"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEE9793974.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            64046..65305
                     /gene="rhaA"
                     /locus_tag="R8G18_000163"
     CDS             64046..65305
                     /gene="rhaA"
                     /locus_tag="R8G18_000163"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEE9793975.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            65547..66371
                     /gene="rhaD"
                     /locus_tag="R8G18_000164"
     CDS             65547..66371
                     /gene="rhaD"
                     /locus_tag="R8G18_000164"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEE9793976.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            66381..66695
                     /gene="rhaM"
                     /locus_tag="R8G18_000165"
     CDS             66381..66695
                     /gene="rhaM"
                     /locus_tag="R8G18_000165"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEE9793977.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            66996..67442
                     /locus_tag="R8G18_000166"
     CDS             66996..67442
                     /locus_tag="R8G18_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEE9793978.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            67453..68904
                     /locus_tag="R8G18_000167"
     CDS             67453..68904
                     /locus_tag="R8G18_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HEE9793979.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            68894..69964
                     /locus_tag="R8G18_000168"
     CDS             68894..69964
                     /locus_tag="R8G18_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEE9793980.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            69964..71712
                     /locus_tag="R8G18_000169"
     CDS             69964..71712
                     /locus_tag="R8G18_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HEE9793981.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(71762..72802)
                     /locus_tag="R8G18_000170"
     CDS             complement(71762..72802)
                     /locus_tag="R8G18_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HEE9793982.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(72955..73788)
                     /gene="fdhD"
                     /locus_tag="R8G18_000171"
     CDS             complement(72955..73788)
                     /gene="fdhD"
                     /locus_tag="R8G18_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEE9793983.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            73982..77032
                     /gene="fdnG"
                     /locus_tag="R8G18_000172"
     CDS             73982..77032
                     /gene="fdnG"
                     /locus_tag="R8G18_000172"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:74567..74569,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEE9793984.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            77045..77947
                     /gene="fdoH"
                     /locus_tag="R8G18_000173"
     CDS             77045..77947
                     /gene="fdoH"
                     /locus_tag="R8G18_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HEE9793985.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            77944..78579
                     /gene="fdoI"
                     /locus_tag="R8G18_000174"
     CDS             77944..78579
                     /gene="fdoI"
                     /locus_tag="R8G18_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HEE9793986.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            78576..79505
                     /gene="fdhE"
                     /locus_tag="R8G18_000175"
     CDS             78576..79505
                     /gene="fdhE"
                     /locus_tag="R8G18_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEE9793987.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(79835..80077)
                     /locus_tag="R8G18_000176"
     CDS             complement(79835..80077)
                     /locus_tag="R8G18_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9793988.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT        19555 a        21275 c        20480 g        19578 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag ttaacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agccaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccgcgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaccc ccacatcatg
    21181 tgggggaaga cagggatggt gtcacaaaaa agtacctaac ctgatgatat aaatggattt
    21241 atgtcaaccg ctgtccacat agcgtccaca tagcgtccac atcgaccata aataagcccc
    21301 tcaactgagg ggctattttt ttgatcacat ccacataatt tgctgccctg acggcaacgg
    21361 gtgcggcctt acggcgtgga cttctcccgg cttcacgatg tatcgctgta ccgactcata
    21421 agtgatgaac gtggcgctgc aattcacgtt ctggcactgg tgataacgct cttttgtcgt
    21481 gtcagtgata tagcgacttg tacgcgcatg tgcggcatgc tggcataaag gacaatgaaa
    21541 catcgcgagc acctcttccg gttttgttga tggtgccatt ttagttaatt tatccttata
    21601 aaacaaacag ataaataaac cacgtcactc atcatcttct gtttcgtact ccacatcaga
    21661 aagccttacc tcaagctcca aggacgtcgt gaagccgcta ttattgagac aatgtgtcac
    21721 cttagtgatg gtccagtcct gctcgtctat gacgcgctta aagcctgaca ctttaaccgg
    21781 tgtttccgtg taaatatctg cacgaccggt agccaggctg atggagaact ccgctacacc
    21841 ccgttgcagt ttatcccact tcgcctgagc ggcgcgcatg gcctgcgctt ttgtggcata
    21901 taccgtggtc agggcaaaaa cgttgtcagc ttcaccggcc atgtattcac cttcgcgcgc
    21961 ttccggtact tttggcgctt tcttctgcgt gaccggtttc gctttcgggt gctccagtgc
    22021 gcgcaggtgt ttttctttct ttttgcgttt cagttttacc ttctgctttt gcggcttcgg
    22081 gtctttggtg tgtaaccact ttgccgttac gccggtgtaa gctccacggt cagcaatcgc
    22141 aaaatgatga cggtcgccgt cgctgcgggt gatggtaatc tgcgggattt ttttaccgct
    22201 ggccgtcacc ccctgccccg ctttgagaaa cagcagtttt cccattttta ccgacacctc
    22261 gccgccgttg cgttcagcaa gacgggtcag gaatttcgca tcagactcct gcgactggtc
    22321 gatgtgtgga atttttattc cggccagtga cggagcgaca ctggcttcca gcctgttacg
    22381 ggaggctatc gcctcaacaa tcgcaccgag cgtaatgtca tgccatgagc cttcacggcg
    22441 ggaattgagc gtcccgcgaa aatctgcact ccgggcgcgg atggtgacca catccggcgc
    22501 gccccggtgt tcaacctcat caacggtaaa tttccctttg cataccaggg caaaaccttt
    22561 ccagccgata tacaccgtca ggacagcgcc acgaaccggc agcccgacct gcccgtcggc
    22621 atcgttcagt tcaatatcaa gctggtcagc ctcaaagccc cggttatccg tcagggtcat
    22681 gctcatcaga cggtcgctga tattgccggt aatatccctg ctgtcgagca tcagcatgta
    22741 atccggcgtc agcgtactgc ctgcatcaaa tgtcagcgca tccagcatta tcccgccccc
    22801 gtcatacccg tgaatttagt cgccatactg ccagccttac cgatgagcga ttccgcctgt
    22861 ttaccgatat cgccataaag cgcggccagt gactcatcaa cgcgggtgag cgacagcgta
    22921 aaatcaattt tccgtggtgt gccgtctgca aagaaaatac tccctgtttc actcaccttg
    22981 ctgatgacat acatgccgta aatcatgccg gtgccatcca gcaacggcca cgcccggcct
    23041 tcctctgcca tcagtctgag cgtggtcatc gtcagctttc cgccagtcag ttcgggataa
    23101 agcacaccgg caagtgtgat gttctcctca cctacaccga gaaactgata ggcatcccgt
    23161 ttaccgatac gggaatttga cggccagcga taatctgatt cacgctgcat ggtctggtgt
    23221 ggcagcgtct ggcgcataaa aacaaacata cctaacgcga gcatcatttt tcgtcacctc
    23281 cttaaccgtc atgcatcatg ctggcacggg cgcgcgcacg tttatcccgc tcgtattttt
    23341 cgagcgcatc ctgtaactgg cggtcaagct gtgtccccgg tgcagtacca cccgtcaggc
    23401 tgatgtgata ttcgttttta ctctggtcca cataagagcg gccagccggt gccgtgaccg
    23461 gctgataagc ctgatagcct gcataagagc tggtcgccgg aatataacca ccggtgccat
    23521 acgtggcggc atgagttctg gcggcggtct ggtcaagtgt gtctgactct ttgttgatga
    23581 ccccgagctt ttccagtacc cagtcaatgc cgctgcgcaa tttgttgaac gcattaagcg
    23641 gcagcatcag cgcgtcagcc agtgcctgcc cgaacatgac gcccgtgtca cggcaacggt
    23701 tcagggtgtc ctgggtggct ttgaccgggg caatcaggtt tttaaaccac tgccacgcag
    23761 cctgtaactt ttcgcccagc cagtcaaaca ccggtttcag tggcgtgaac agctccccca
    23821 ccggcgcaaa tgccgctttc agcccttcaa ccacaccgcc aaagaatgcg ctgacaggct
    23881 cccagtattt acggataagc aacgccccgg cgacaatggc ggccaccaca gccacaaccg
    23941 gccagctaat cgccccgatg gccgtcataa cggcactacc aaccgtcgtg aagattgccc
    24001 ccattgcgcc tgctgccgcg atgatggcat tgatgccggt gataaccggc caggctacaa
    24061 gaccaattgc accgatgatg ccagtcagcg ccagcgcgcc accggcaatg aggccaatgg
    24121 ttgacgccag tgatttgttt ttctgtaccc agccgtcgag ttttaacaca tactttgtgg
    24181 ccgtctgcgt gagcttacgt agcgcgcctt cctgctggtc aaacaggtct gtccccaccg
    24241 cctcataagc ggactgaaac tccttaaagt caccgccgag gttgtcctgc atgatattta
    24301 ccagctctgc ggtcttcccg tctgaggctt taaacgcagc ggtcagtttg tccagctttc
    24361 cggttgacgc ggcagtcatc agcacagcgg cggctgagct ggcctcctcc ccgaaaatgg
    24421 ttttcatgta ttcggcctgc tgggcagtac cgagccggtt tttctcaaag ctggcctgca
    24481 tttctttcag aatggtaaat actggtcggg tgtttccctt actgtctgag gttttcacgc
    24541 caagctcttt gagtgcatcc catgcttttc cagtcggtgc ctgcaggcga cttaacacgg
    24601 cacggcttcc cgtccccgcc attgagcctg tgatttttgc atcatgcagc gccccgacca
    24661 ttgcggcggt ttcttcaatg ctgacaccgg cattttttgc cactggtgcg gcataggtca
    24721 gcgcatcgct catgccgtca aaatcagcgg cggttttgtt catcgtcatg gagagaacat
    24781 ccccgatatg agcgacctta tcgtttgaaa gctgaaaggc ggatttcatc cccatcagca
    24841 gggcggcgtt ttcttccatt gtgcggcggt tcgccagcgc catattcagc gtgaccggcg
    24901 ttgccgcctg aatggcatca acatccccac ccgctttcgc aatgataatc tgtgcaccgg
    24961 ccgcatcatc cgctgaggcg gcggtgttgt caccgagctg gcgcgcctgc ttgcggagtg
    25021 cagccatttc ggcggagtct tttgccacac cgagcacagc ctgcaattct gagtttttct
    25081 gcgcaaactc ataacctggc atcagcagct taactccggc tatcgttccc gcagcagcaa
    25141 tccccacacc ggcagcgccc actgaggcca tatttccggc cagttccttt ccggcctgat
    25201 aacgctgttt gactgcgtta agttttgcct gttgcgcact gacacgcgcc agcgcgtcac
    25261 gctgccggtt aagctgggcg gtggtttcac tgatacggtt tttcagtccc tgctcatcat
    25321 gtgcaagatt gcgggtatta attcccacag cggccagttc ccgctgctgg cgtttaacgg
    25381 aatctgtcag gcggttatat ttcgcctgta agtcctccgc cgcacgcttt gcggattcca
    25441 gcactttcgc ctgagcacgg gtcgggcgtt cggtgttttt aaactgtgtg gcaagggctt
    25501 cggcctcctg ccgtgccttt tcaagtgcat gaccagtcac ggcgagctgt gcactggtct
    25561 tgcggaatcc ctcaatacgg gatgcgtgac cgttcagctc gcgcagtgat ttttgtgttt
    25621 cccggatatc ccccgacagc aacttactcg ctctgcggat ggatttaaac gggcgggacg
    25681 cctggtcaac agccctgagc aatacctgta attttacatt gttactcatt cgtgtttccg
    25741 cttcgcctga gcgccttttc gcgccatgtg atgagttcgg tcaggctcat gggatacagt
    25801 tctgatggcg gccagtgaaa tatcactgcc acatccgcca tcaggtcatc gaccgacaga
    25861 tttttcggaa gcgtcactgc accgagttcg gcgacaaaaa accgaccacc ttaccggcca
    25921 gcgccacaag gtcaggcagt tccagcgcgg cgacttcctg ctcggtcagc atcggtgccg
    25981 tcatgcgcgg cagcacctta atcagtgcat cgacttctga gtttgcgacc gcagccagac
    26041 tgacaccgcg cagcgtcccc gcactgggtt tcatcagcgt gacctgttcg ataacctgct
    26101 caccacgctt gaccggattg tccagggtaa tcacattttc tttgttcatg gttttctcac
    26161 ttctgaatcg gggttaaccg gtcagccagg ctgaccggat gaaaattaca ggccgatatt
    26221 gcggcggtgt tgctccagcc ggtcgacgcc gttcaccttc tcaatcatgt tgatggtgtc
    26281 gatttcgacc agctccttac cgtccatcgt cagccggaaa taggtgcaga ccacggagat
    26341 tttcgactcg gtgtcttctc cctgtttacc ctcgccggtg tcgatttctt tctgacggcc
    26401 acgcatgacc acctctacgg ccaccgtttc gccggtatcg tcacgctggt aagagcctgc
    26461 aaaacgaatc ggcacggcat ccacaccggt tgcggcgtaa agctcccaga taaccgaatc
    26521 cgggaagccc ccgagcgacc actccattga cagcgcatcg tcatcaaggc cgaggtctac
    26581 cggtgcgctt ccgttcatcc ccgcaccgcg atagttttcg agtttacggg tcagttttgg
    26641 cagcgtgacg gactttgcaa cgccctgata gctgtagccg ttcagaaaga cgttcattaa
    26701 cttgagtttg cgcggcattg ccatcggtca ggctccttaa ttgctgttaa ccgaggtgac
    26761 cagatttgcc aggtatttat cggtaatacg ctggcgcagg gtcaggtttt caagaggagg
    26821 caccggggta tagtcgtagt cgatatacag ttttccggcc ttgagggttt ccgcatcgtt
    26881 ggattcttcg ctgaaccagc aggtcgcatc cacgatatag ccgtttgttt tcagctcacg
    26941 gaatttggca ttgatgccgt caacgatgtc gcgaatcagc gttgcggtga tgggcttgtc
    27001 caccgcccac atgtgcgcct cagccatcgt gtcggccagc acctgcgcgg tgcgggtgta
    27061 gttttcaaag aggaacagcg ggtcatcaga gcaggtacgg ttaccccaga agcggaaacc
    27121 gtcacggcga atcagcgttg tgacgcctga ctcgttaagc aggtcagcat cggtgccgga
    27181 ctcctgcaaa tcccagaata cggacgcgct gatgccggta acaccgttca ccccgacgtt
    27241 ggacagcgtt ttatgccagc cctgctcctg gtcgatttta gcgcgcagac ccagcgcacg
    27301 ggcggtggca tacgcggtgg cggtggtact ggcgaccgta tcccatgcga ggaaatccgg
    27361 ccagatgacc atcagctcac gctggctgaa attctgacgg taggctttca cctcggaaac
    27421 ggtcttacag ccccatgcgc tgatataccc gaaagcgcgc agcttctgac agactgatgc
    27481 cagtgcaaca gccacctctt tggtgtccag tcccggcacg ccgagaatac gcggtttaac
    27541 accggttacc gactccgccg ccagcagggc tttcagtccg gtgtactgac cgttttcgtc
    27601 ggtggtgccg atgatattgg aaacggtctg cgcgagtttc gtttcctcgt cgtcgccggt
    27661 gccgtcttcc acacgcacga caacggtgac cggttttgac tggtcggcga tggcctgcaa
    27721 cgatgccgcc agcgtgcctt ttttaccggc ctttgcaatt gcgctctgca cattggtaat
    27781 cagcaccggt ttattgaggg ggaaggtttc cgcatccgca tcgctggccg tgcagaccat
    27841 gccgacaatg gcagtggata cggtggaaat gacgcgggtg ccgtcgttaa tctccagcac
    27901 ctgcacgccg tgatgatagt cactcatccg tttaactccg tggttaatgg gtgcaactat
    27961 tttctgttgg gcagtgcatg agacgctatt tgacctggct ggtcagtgga tgaaacaaca
    28021 gataaagaaa atgcgggcaa ttcgcccgca tgtcctgatt tgtacacact cattttccga
    28081 ctgacagttt acatagccca aacgctatca aatctgacag cctgctatga gcgaaaagcg
    28141 ttaaatctca cgatatctgc taccgggaaa aatttcgatt atcttcgatg ggaactttga
    28201 taatgtagta aatgccattt tcagcgatca cttttcaagc gtgtgtaaat gattaatgtt
    28261 aaaaaattga cttcactgaa ataaggttgc tagtattttt ggagcaacca ttaatgtttt
    28321 ttatttaagg aatgtaaatg acatacgcac tgattgataa ttcaactcta actgcagtcc
    28381 aaagaatcga ggggaaagta aaaacaagat cgaaggactc agtagataca gacataatag
    28441 catttgagaa ctatatacag tctattttgt tctatgatca cgttatcgcg gttgatgatt
    28501 atattccaga gtaccgtggg agtaggattt caagtttcag agagatatta tttcttgata
    28561 aagcacaata tgggttggat gaaattgaaa gtgaagcaaa aaaaatatct gatgcattga
    28621 aacctaaaat tcgtggtgga acatttgtta atgacgattt taaaaaacta attgaacttc
    28681 ttcagacaca tatcatatgt acatgggaca ttagttcaag tgtttatcat ctaacactga
    28741 agaacttatc tgatggaggt aaggagtttg aaaaatatgg taacattgct gctgccattt
    28801 tttcagaact gcgtgatgct gctgagtctg gtaatagaac aaatggcgat gttgaacttc
    28861 tcgatcgatt tggcaaccct attcgcaagg ggtacaaagt tcccggagca aaatgggggg
    28921 atggcacatc tgctggtgaa gcatcagatg caatcaaagc attcgtggct tcgcttgtat
    28981 ggctggctaa tcgggcagta ttttattctc tcacagctaa gtaccttcaa gcagatactt
    29041 ttttatatcc attacgtcag gcgtatcaac agacatattt aagccaaaac tgtcagtatg
    29101 gttttgacta tgctaaacat attgtagaaa acttttccac ttcgttaagt catgatctca
    29161 ttgatattca ttctggtggt aaagctgtag caacagcaac ttcgcttcct ttattttcgg
    29221 catggctcgc aaaagaaact ggtgatccaa gttgtattgt tgaagctgct tataacataa
    29281 gggacaacaa cgaatttgta agggcccgtg agaaaatgcg agaaataaag cgcttgtttg
    29341 atgaaaatga attgcctcaa gctaataagt cagtaagagc attgcttact gaagtatcga
    29401 aagaatcaga ttatattcga actaagtatg ggctcaagac tagacaaggt gtacctgtaa
    29461 ctaagcttgt acatgtatac aatactgttg cggctattaa ttcccttccg aaactaccga
    29521 cctttaattt taaagttaaa gttcctgatt tcttatatga tcttaaaaaa cccaaggggt
    29581 ttcgtgctct ctatcgaaat ttgactaatg atctttctac tgtgtggtcg ttaggagaag
    29641 ctcgagatat tcttggaagt aaagttgtga tagataatca tgcaagaatt tacagtccga
    29701 agcatgaaga accgcgtttc aaaaactctc attccccata taagagcccg atgtagctta
    29761 agaaaaagac acgcccacct tgtgcgtcta cttctggcac agagcggact ctcagattag
    29821 gctttactct gtgccataga tatgtaagct cacaccagag ctcatacaac ttattgcggc
    29881 atttccggcc attcaggatt tgcaggatcc acacgactga ccagaacact gtagcgttcc
    29941 catgcctcca gtcgactgcg ctcctcatct gttgccatat tcagcctgac agcgcgctcc
    30001 agcggcaaaa tcacggattc agcatctgca agaagtctgg ctttccgatt ttctgcctgc
    30061 tgctgcaatt cctctgccgt ataaattcgt ttaatcactg tacagtcctt aaacatccag
    30121 ttccctgaaa tgtccgcccg tcggttagca gtaatatccg gcacttcaac aacacttaat
    30181 ccatccggtc tgatagctgt cacatccttt tccacatagc ggataatatt atctttgtcg
    30241 tacgctattt ttatcgtgtc atcagcaaaa tacttttgtt cttcgtacca gttcttacca
    30301 tcttctgaaa aaaaccagac aacatcaaag tcctttgtca attgatattg ttcaaccgtt
    30361 tttggattac ctgccgttat atttatcaaa tgctgcataa attatacctg tgccacgtta
    30421 taccaagtcc cgttaatgta tttctgcacc ggtctgtaat atacgccacc aatgttatcg
    30481 gcagagtttg agccggtatc ctgaacaata atgccggaat atacacaccc ggacggtgcc
    30541 tgatgtgtcc atgtcatacc attgttcgca ggtttgtatg tggcagcacc accaagccgg
    30601 atatcccgga catagcgtga atcaaagttg ccatagttag atggtgatac ctgcccgtta
    30661 acagcaaaag tgatgctgtt atctgtattt ctctgactgt aaaaatgcca gcctgcatca
    30721 tcacctaatt cagccaccac aggacgactt gagtttcccc acaaattgaa tgcggcttcc
    30781 ttcgtggatg tattgctgct gctgaccgtg aactttttcc cgctaccggc acgtactttg
    30841 gtacttgaga caatatcacc tgtaacactc aggccatgcc ccattgacac tccgccattg
    30901 gcgttattga tagtcagcgg tcttaagccg ttccatgtcc caaatttatc gccagaggcc
    30961 gtcagcatta aatatgtgct gccgccatca ttcctgataa agaatccata attgccataa
    31021 gcaatgcgca gaccattagc actgagtgat gtaatttcac ctcttgaacg gagaccataa
    31081 gcggagctga gtgataattc ttcctgagcg tcattgtttc ctgtcgccca gcgaattacc
    31141 ccgccctgaa ctgtttcatg ccagatagta tctccttctc caccacgaaa ctttctgaga
    31201 tattttttgc cgcctctggt gcctgaacat aaggccgtag acatataggc attctggctt
    31261 ccgccatcct gattaatcgt tccggtcatt gagtcgccct gacgattcca gtcacgacgc
    31321 cagccggggg agtagccgtc cccatgattt atgtaagtga attgcgcgct ggttgtaccg
    31381 ccaccgcttg atgttgttgg cgtggtcact cggatagtga ttgcagattt agttcccatg
    31441 acctcgacga cacaaccagc caggtggata tcaccacatc cggtatccgt aatgattttg
    31501 ttatttgcat atgaccagga gcctttgcac atccagtacg gatgattaaa tgcaccacgg
    31561 gaatccagcc attcaataaa ctgagcggtt gtccagtttc cggcttcagt gctcaaagcg
    31621 ccgctataag cacgacaggc accgatattt ttcgtgaagg tatcctttcc cggaatatcc
    31681 gcaccgttct gatctttctg aagacgtttt tcagcattgt cataggcaga cttcactgct
    31741 tttggcgttg cggccagcgt ttcagaatcg ctgttgatgg cactacttag ctggacaaga
    31801 ccttttcgcg ctgtggtggc atcctgtgca gtgtatttcc cgttagcaag gtcatacgct
    31861 gtcttaaccg cctttggtgt tgccgcaagc gtttcagaat cgctgttggt ggcgctactg
    31921 agctggacaa gaccttttcg cgctgtagtg gcgtcctgtg cagtgtattt cccgttagca
    31981 aggtcatacg ctgtcttaac cgcctttggc gttgccgcaa gcgtttcaga cgtgctgttg
    32041 gtggcactac tgagctggac aagacctttt cgagctgtgg tagcgtcctg tgcggtatat
    32101 ttcccgttag caaggtcata ggcggccttt accgctttcg gcgttgcggc cagtgtttca
    32161 gacgtgctgt tggtcgcact gcttaactga gtaaaacctt ttgcggtcag cgaggcgtcc
    32221 gggtgacgtc gtgactgttc gtgctctgca attttgtcat caacgtaatc ctgtgtcgcc
    32281 atcaccgttg tggtgtcaat ggtcagctcc accgaggcca cactgctgac gatgatgacc
    32341 atgcgacagg tctgcgaacg ccctgagcct tcggcaagag ctggcttata actttcggcc
    32401 atgttcgcca cggcaattaa cgttcccgca tcatcgtaca ggccaagctc acgcatccag
    32461 aaaccgccca cctccggcgg aataaccagc tctgcgataa tataattact gtttcgtttg
    32521 tcctggctga ttttgttcag cgtatgtcgc cagacttcgt ggataagccc ggtctgtccg
    32581 gcatccggga caggcaattt accaccgcca tccccgacgg ccatcgtggt aatgttgacc
    32641 ttccgccctc ccggtgcggt tgccgctgcc agctttgctg caccggcagt ggtgataacg
    32701 gttctgaatt ttgtgctcat tattcctcac ttatccgggg taaaccgtaa ttacatcgcc
    32761 gtcgtaagcc acaccaccgg cgaacaggta gccgggaatg tcccgggtaa tgttcaggcc
    32821 aataagatga cggcttgcag gtttggcatc agcaataagc cgttccattt cctgatacat
    32881 tgcctctgtg atgccgcttt ccagtacacc aatatcaagc cggaaggtgc cgggcgggtc
    32941 actgttttcc caccactccg tcacgttgat gagatagccg agcggctcca ccacacgccg
    33001 gattgcgccg atagtgcctt tatgacagtg gatgaaatag gcatcgcgga taacggcgcg
    33061 ttttgtcgct tccggccact tttcatccca cctgtcgacc gaaaacgccc acgccagcca
    33121 cggcagcaga tttgccggac aggtatccgg gttccacagt tcacgaatcc tgaccggcgt
    33181 tttttcaatt tccgcacagg cttttgcggc ggcaacttca agcggtgatg agccggtcgg
    33241 cagcagtcgc gaatcactca tccgagcctc cggtcacgac gcggtattcg gtacagaaag
    33301 acgcctgcgt actgttgagc acgatgtcgg ccagcggtgc agtcagttcg acacgctgca
    33361 cgccttccac atgcaaagcg gcataaatgg cagacagacg gatgtcgcgc cccagccggt
    33421 gctgtgccgt gatgtacgct tccagttttt tcacggcagc agcgcgtatg ggttcgcttt
    33481 cgggaccagg gtaaaggtaa agcgtggcgt ttatctggta ttcaacgatg gcggcagact
    33541 gcacggtcac gcggtcggcc accggcctga cgtcctcacc attaagggcg ttacgcacca
    33601 ccgccagcag gtcttcggat gcgacaccgt tattttcacg tgacagcaca gagatggtga
    33661 cgcaggccgg agacggactg gttacagaaa tatccgcgac acgcccgtcg gcactgcgac
    33721 catgatactg ataggccccc accgacccgg cgacgcttaa accttcaaac gcctgctgaa
    33781 tacgcagacg ataatccgtg tcagattcca tcactgccgg tgtcggcggg agggtcgaat
    33841 catctgccgg ggtgataatc aggcgcgtgg tgttgtaatt ggcaccaatc acatcaaggt
    33901 cattaccggc ggcacaggcc agcattaccg cccgtgcggc ctcattcaca cgctgacgcc
    33961 agataagctc acgataagca ttttcctcca gcagtttgac gagaggctcg gattccagcg
    34021 tcagggtacg ggcgaccgcc tcctgctggt cttccgggta aagggaaatc agtgtcgcct
    34081 tgcgttcggc aagaatggtt tcaaagtcca gctcctcgac cacatccggt gcgggtagct
    34141 ggttcaggtc gataatcggc atggtttcaa ctcacaggga tggttaacga aagtggctgg
    34201 ccggtgtcgt tgtgctggcc ggttaacgtg accgtcattc gcccgtcaaa gctgcgcgcc
    34261 gtggtgacgg atgacagggt gacgcgggct tcccatttca gcacggccat gtaacaggcg
    34321 accttaatct gcaactcaag cgccggggtc tgcggctggt caatcattga cgccagcaac
    34381 gagccgtaat cacgacgcat cacccgtgag ccgaccggcg tgcgcaggat atcgccgata
    34441 ctctggctga tatgctcaag gtcagagaca gtcaggccat cactgcgatt cattccgaga
    34501 taacgcgctg tcatagagga ctcccggttg tgccgccgct gtcgccgggg tgtttatggg
    34561 tatgcagtac cttcccgttt gatgagagtt caccgccggt gtgttcaatg ttgccgcgca
    34621 tcgtcccgcc cttctgcact tccagcgtgc cagtgatcag cttgtttgtg cagaccacct
    34681 ccggcgtgtc cagagtgacg cgggttgatg ctttcaccgt gaccaccggc accgtggcag
    34741 taacagaatc agaagccgtc acgctggccg ttttaattcc gcttaccgtg agtgcactgg
    34801 tttcaggttc atactcaatc accgccccgt cagggaaacg gatatgcagg gcatccgccg
    34861 acgcagacgg cgcggggtta tcaccggaat aaatccccgg cagaacgaac gccgtgtcga
    34921 gttcaccgcc tacggccaga atcagcacct gttcccccac ggaaggtgcc caccatgtgc
    34981 gcgaacgtcc ggcgcgatgg gtcagccact gaagccagtc ggtgcacatg ccgccggtct
    35041 gcacacggca gcgaccggca ttaaggtcgg tttcgacgat aatgccggtg cggatcatgt
    35101 tgcgcagtgc gcgcgcgagt tcctgaatat ttgcgagagt gttcataacg ggaaggatgc
    35161 cgccgggtca taccggcggc aatgtgacga tgaggtgtca ggaatggcac aactaacggt
    35221 cgaggtgagc caggataatc tcttcaatca tctgcacatc ctcaccggta aagccgagca
    35281 ggggacgcgc cggataatga attttcttac cgtctttccg ggtttcttcc gacagaccga
    35341 actgatgcac actggctatt ttcggtgact tcccgccgta aaactccatt gatgcctgtt
    35401 cagggctggc gcggatatgc aaaaaacgac tggtgataag tttcgcaaac atttttcgct
    35461 taacgcgacc ggtctttttt ctggcgctct gctgctggcg tggtgcgtag ggtgtgccgt
    35521 ccggggcttt ctgtgccatc acccgacgct gctgactctg acgcagacgt ttcgccagtt
    35581 cggcgctcag tcgccgacgc cctgacggtg acagcgactc aataagtccg gtcagcctgt
    35641 cttcaaaacg cttaaactca ttcatcccac ttactcacca gttcgccatt gatataaagc
    35701 tccatcgggc gggtgaccgg ctccggcggc ggaggttccg ggatattctt cacatgcagc
    35761 gcgccgtcca cctcactgac cagcgtgcgc tcggtcagca tcaggctgat gctgatatca
    35821 aagctgctgt cattgttgat gtctgcataa aacgtgaagc cctttttctg gccttcgtcg
    35881 gtggtcatga tgtcgggctg attttcccgc agccacgcca gcaccggcac gatgagcagg
    35941 tcaaaatcac cggtaaagtc ggtcacaatc acgttgagcg tgtaacgctt ttcgaatgac
    36001 agcgacgtcg ccagtgtgga ggcaatactc ccgttatcca cgaatatccg cagcatctcg
    36061 gggcttgttt tcagcaccgt gacggcatca gtcagcgccc tgcgcaggct gtcgggtttg
    36121 agcatcgttt tcgtcctgac agtgtttaat catttttacc tggctggcac agcgtgccag
    36181 cgcgttctca agctgccgga tatcagcact taaatcgccg ttcgtctgcg ggtcactgcc
    36241 cggcatcggg caaaggctca ctttcgggca ggcgttgtgg acaatcactg gcgtctgcgc
    36301 aggctgtgcg ctggtgcaac cggcgcacag catcaggcag gtcagcaccg taccagcggc
    36361 gaaaatcttc gttttcatta agtaacctcg tgatggtttt ctcgcgctga gcttcacgct
    36421 tcgcggcgtt ctccagttcc tgacgcagtg ccacctgcgc cagctcgttt ttgtctgccc
    36481 tggtgagggc aacatgaagc tggtttttca gcatggtgat ggtcgtctgc tgttcactgg
    36541 cgacgttgtt cgccctgtcc agcaaggcgc gcaggctggc atttttgtgt ttcaccagaa
    36601 acagaccggc caccgccagc gataacaaca caaccagcac aatcatcagt tttgacatgg
    36661 ttcccgcccc tcaagacgct gacggcaggc cgtacgtatc agccggaaaa acagcgatgc
    36721 cacgagataa atcagtgcgg taaaaatcca cccggcagcg accagcgaga taaacgtcgc
    36781 caccatcacc atcagagccg ctgcccgtct gcgccacggc accggctgca aaaacagcga
    36841 cgtgacaatc ttcacggcca gcgattccgg cggcagctcc cgcccgtagc gttccagcac
    36901 atactccgcg gcatacacgc cgacaccacc ggcaatcaca cagataaccg tcgccagaat
    36961 cgcccatgca gcgacaaaac tgacggccac gctctgcggg taaatcaggg acagtgccag
    37021 catcagcgcc agcgacacgt tcagcatcag tgaaagggat aatttcttca tggtgtttac
    37081 tccgtttaag ccggtacgcc gccagcggtg cgccagacgg tgaccagttt ttccagtgaa
    37141 tgctcacgct gaccgtaacc ggcacccggc agggacgccc agatattgcg acagcgtgaa
    37201 atggcgcgct caatgcgtcc cgcccggatg tcatccagtg caccgcgttc gcggatcaac
    37261 tgaatggcga gcctgtcctg tgacaacgga ctgaaatccg gcagggcaag ctgtttgcgg
    37321 tagtgcggcc agaacaggta aagctgctga tagcgaccgg aggccgtgga tttttcaccg
    37381 cgacggttaa acaccttcgc cggtcggcca tgtgcgaacg ggtggtcact gtagtcggtg
    37441 aagatttccg gcttcccgtc cagtccggtg actatcacgt catagccccg gtttttcgtc
    37501 agcggatgat tcgccgtccc ttcggacacg gccagcatgt cgagaaaggc ggcgatattc
    37561 tgatgtgtgc taattaccgg cattacggtt tccccctgcc cttaaagcgg cgctgaatgg
    37621 caatctcaat cacctgataa ccggcgatac ccagcatgga gccgatgccg cacaccgcag
    37681 gcagtgacag gtcaggaaac tgcaccagaa caacaccggc aaccatcgag acaaaaccac
    37741 cgagcaacat gcgcccgata aacagacgcg gagtgatggg ttcaccaccg gcaagcacct
    37801 tgccgacaac aatcagcacc ccaatcatga aaagcgacag gacgcttttt tcttctgctg
    37861 tcatgcgtta ctcccacaga ttgacagttt cagccacggg cgcggtctga acgtcgggca
    37921 gttcgacggc ggtgccgtgt ggcagcaccg cccccagttc agccagtccc ggatttgcgg
    37981 cgagcacggc ctcgaccacg ccctcagtgc gcccgtaata ccgaacacaa atggcgtcga
    38041 gcgtgtcgcc ctgtagcgca aaggtcttca tcagatttga ctcacgatgc agcgcggctt
    38101 gtcctggata cgcgccaccg cccagcgcat atcccgccac agctcatcaa tggtgctgtc
    38161 aatgctgtcg gccttcttgt cgcccttggc actggcatcc acgccgcggt aacgctcata
    38221 aagcgacgcg gtcgccatcg cacacacagc gcgctcgtag taaaaaactt tgatgctttc
    38281 accgtcgatg tcgtccgccg ggacgtcagc cagacgcgaa aaaccggcag caattttctg
    38341 ttcgcggtac tcgtacagct ccgcattcgt ctccgccatg cctgacttga tggcctcacg
    38401 cagacgggcg ggggcgacgg tctgctcaag gcgcatacgt tcccggacgc gcttcgggtc
    38461 aatatcggga aaaaagaacg tgtttttaat caccggctcg tcgcctgccg gttgcgggat
    38521 aaccaccgta ccctcaccgg acacgggagc ctcctttcgc ggaataatca gcgtcatcat
    38581 gactacctct gaaaagtcgg gcggtggacg ccggtgcagt gtcaggtgat tcaccgtcac
    38641 tgaccggcgt gccgccctgg cgcggggcgc attcggttgt taactggctt tctttttcgg
    38701 gcgtccacgt tttgccggtg tcacgctccg ggtcttacgc ggagtgcggg tggccgcttt
    38761 gggctgcggc tccggcttcg gtttcagctc ccgctccagt cgttcaatct cttttttgac
    38821 gcctgcctga cagtcgagct gtgtcgcacg ttgcaggtgt accagcgcac ctgcggcatc
    38881 accagcgtca cgcagaaaca gaccggtgat tttgtgcagc tttgcgcgca cttcatcagg
    38941 catgtcagcc gtggcggtca gttcgagggt gtccgtcagc aggcggggat ccacagactc
    39001 accggcagcg tgagcgcgca tggccgcaag cgccacctcc tcggtgaaca tgtacggcgg
    39061 ggtgcggcgg tgtttacccg gcatggtcag accgtacttc agggcataac gggcaatctc
    39121 cagcgcaccg gcaatatcgc cggtatccag acgccacagc atgaccgtca tcagaatgtc
    39181 atcctgtgca cctttgccct gctccagcac gccgttcacc cacggcaacc agaacggcag
    39241 cagttcgcgt tttttcgcgg ccttcagctc ttttgaataa atcgctttca gtgtgcgctg
    39301 gtctgcggcc agcttgacca gcatctgctc atagacagtt gcatgtcgca gcggggcggc
    39361 ttcccgctgc gcggtcatcg ctgccgagac ccgcatcatg tggcgctgtg cgggactcgt
    39421 catcggttac gctcccggct ctgcggtcgc tttagccggt gtggagaaat caccgacctt
    39481 aattttttcc accagacaac cggcggcgta gtcctccacc acgtaatcaa tgttcattga
    39541 ctcgtagttc tccacgcggt cgagtttcgg gttttccaca atcacgcggc gatggctgtc
    39601 atccatgtag tagatggaca ggttttccag cttcgtgatg agcatcgcat ccgccgggaa
    39661 gtacgggacg cgtaccgccg gcaggttacc gatgcgtttc tggctgatga tgacgtcagc
    39721 ggccagcatc tcgctgttat cctgctcctt gttgacgata gggaaatact tgtccgccag
    39781 tagctgacgc cccacaatca ccacaaggtc agggtcttcc tgataccaag gttcaatcag
    39841 gttgttggtc gcatccatca ccagtgcatc gaggcttaca taatcaccgc ccttacccac
    39901 gcggataacc tcagaggtgg tgcggccttc ctcgtcagtg accttgctca tcacgcgcgc
    39961 cggggcttca ttcggtattt ctgcagccag ccgaccgcca catcctgcag catcggatta
    40021 ctgttgcggt cagaggtttc ggcacgcttc acgccgttaa aaccggccat gatgaaatca
    40081 agggactggc gtttgataat ggcgttacgg atacggagct ggaaatcctg ataacgcgcc
    40141 cacaggtcaa gcgttttgta gcggatataa aaatcgaagt taacctggtc gcattcgtac
    40201 ttgtttgacg ccagcttcga gaagtccttc ggctgacgct cggtgccacc ggcggtgtcg
    40261 gtggtgctgg cgatggagcc ggtgacaccg atgccaattt tttccccttt catttcactg
    40321 accggcacaa tgttgatgcg ggtcagaaag tcagaggact cctgcatggt gttcatcagg
    40381 gtctgggtga ccgacggttc aacggtgaat tttttcgaca catcaccggc gtcgatgccg
    40441 ttcagctcgg caacacggga caggtaagca ttaaatttaa agcgggtttc ctggcgcata
    40501 gtttttcctg aaattaaggg ttaatcgtga aggttttccc ggactgactg acgccggtca
    40561 gcagttcgtc atcagggcgt caccgccacc accggtggcc ttgctgcggc gctgctgggt
    40621 cagactttcg gtgtggtcga gactgttttt caggcgggtg aatgcctggc tggtttcatc
    40681 cgccctgtca gtcacctcct gcttaagtgc agaaaaggcg gtttccatct cagcgaggcg
    40741 ctgctcagtg gcgctcagtt tttcctgcac atgttcagca acagcggtca ccgcttcatg
    40801 cacgtcattc agacgggcat catcgctggc ctgtttgcgg ccaaaaatgg atttcacctt
    40861 ttcggtcagg gcggtgaaca cggtttcagg caggtcttca aattccagct caacaggcgt
    40921 tgccactgaa atcaggtttt cagggcttaa tttgaagcgg ttcagggggt tgtgttttgc
    40981 cgtgcggcag aattccaggt attccgtgcc gaggcttgcc gggtcatcgg tgacggccag
    41041 acccaccaga taacatttgc cggtgttggc aaagttcggc tgaatttcca ttgaggtgta
    41101 gaccttctgc gcggccttgt tcatcgcgat aaggtcatcg gtcggggtga ttttcgcaaa
    41161 cagcgcccat ttgcctttca gcgccgaatc atcgtcaatc ttttcggcct tcagttcggc
    41221 cacatcgcca taacgcttaa aaataccgtc aggcaggatg ccgcgcagat gttccaggtt
    41281 aatgcggcaa ccatagacac gcgggtcaaa ggtttcggcc atttcctgaa tatcctgcgc
    41341 actgatgaca cgcccgtcac aggtgtcacc ctcaacgccg atacgaaaga attttgagac
    41401 tttttttgcc attgtcagga gtcctgaata gtgattagag gagtcacatg tcggcatcag
    41461 tttcccgacg atgcgcatcc tccgccatca gtcccggatg gtttatcact gacacaacag
    41521 cacattagcg aatcgcgggg cgcgactcag tagccttgcc gtgtattcat cacggcgagg
    41581 tattcatgac catcaccaca gacaccactc ttttacacga cccgcgtcgt caggcggcgc
    41641 tgctgtactg gcaggggttt tccgtgccgc agattgccgc catgttgcag atgaaacgcc
    41701 cgacggtgca gagctggaaa cagcgcgacg gctgggacag tgttgccccc atcagccgtg
    41761 tcgaaatgag tctggaagcg cggctgaccc agctcatcat caaaccgcag aaaaccggcg
    41821 gtgacttcaa ggaaattgac ctgctcggac gccagattga acgactggca cgggtaaacc
    41881 gctacagcca gaccggcaac gaggcagacc ttaatccgaa catcgctaac cgcaacaaag
    41941 gcgggcgtcg caaaccgaaa aagaattttt tcagtgacga ggccatcgaa aagctggagc
    42001 agattttctt tgagcagtct ttcgactatc agttgcactg gtatcgtgcc gggcttgagc
    42061 accgcatccg cgatatcctg aaatcccgcc agattggcgc gacgttttat ttttcccgcg
    42121 aggcgctgct gcgcgccctg aaaaccggcc ataaccagat ttttctgtcg gccagtaaaa
    42181 cgcaggcgta tgtgttccgt gaatacatca tcgcctttgc tcgtctggtt gacgttgacc
    42241 tgaccggtga cccgattgtc ctgggcaata acggcgcaaa actgattttt ctcggcacca
    42301 actccaacac cgcacagagc cataacggcg acctgtacgt cgacgagatt ttctggatcc
    42361 cgaattttca ggtactgcgt aaggtggcat caggtatggc ctcacagagt cacctgcgct
    42421 cgacctattt ctccaccccg tccacgctgg cgcacgacgc ctacccgttc tggtcgggtg
    42481 aactgttcaa ccggggacgc gccagcgccg ccgaacgtgt ggaaatcgac gtcagtcata
    42541 acgcccttgc cggtggtctt ctctgtgcgg acggccagtg gcggcagatt gtcaccattg
    42601 aggacgccct gaaaggcggc tgcacattgt tcgacattga gcagctcaaa cgcgaaaaca
    42661 gcgccgacga ttttaaaaac ctgttcatgt gtgaatttgt tgacgacaag gtatcggtgt
    42721 tcccgttcga ggagctgcaa cgctgcatgg tcgacacgct ggaagaatgg gaagactacg
    42781 cgccgtttgc cgcaaatccg ttcggctccc gcccggtatg gattggttac gacccgtcac
    42841 accgtggcga cagcgccgga tgcgtggtgc tggcaccgcc ggtggtggcc ggtggcaaat
    42901 tcagaatact tgagcgtcac cagtggaaag gcatggactt tgccacccag gcggagtcca
    42961 tccgcaaact caccgaaaaa tacaacgtcg aatacatcgg tattgatgcc accggcctcg
    43021 gtgtcggcgt gttccagctc gtgcgctcgt tctatcccgc cgcgcgcgat atccgctaca
    43081 cgccggaaat gaaaaccgca atggtgctca aggccaaaga cgtcatccgc cgtggctgtc
    43141 tggaatatga cgtcagcgcc accgacatca ccagctcgtt catggctatc cgcaagacca
    43201 tgaccagcag cggacgcagt gccacctatg aggccagccg cagcgaggaa gccagccacg
    43261 ccgacctcgc ctgggcaacc atgcacgccc tgttaaatga gccactcacc gccggtatca
    43321 gcactccgct gacatccacc attctggagt tttactgatg agcaagaaaa aagggaaaac
    43381 accgcgacct gcggcaaaaa caatcaccgc cagcgccccg aaaatggaag cattcacctt
    43441 tggtgagccg gtgccggtac tcgaccgccg tgacattctg gattacgtcg agtgcatcag
    43501 taacggcaga tggtatgagc cgccggtcag ctttaccggt ctggcaaaaa gcctgcgtgc
    43561 tgccgtgcat cacagctcac cgatttacgt caaacgtaat attctggctt caacgtttat
    43621 cccgcacccg tggctttccc agcaggattt cagccgtttt gtgctggatt ttctggtgtt
    43681 cggtaatgcg tttctggaaa agcgttacag caccaccggt aaggtcatca gactggaaac
    43741 ctcaccggcc aaatataccc gtcgtggcgt ggaggaggat gtttactggt gggtgccgtc
    43801 cttcaacgag ccgacagcct tcgcgcccgg ctccgtgttt cacctgctgg agcctgatat
    43861 taatcaggag ctgtacggcc tgccggaata tctcagcgcc cttaactctg catggctgaa
    43921 tgagtcggcc acgctgttcc gccgcaagta ttacgaaaac ggcgcacatg ccggatacat
    43981 catgtacgtc accgatgccg tgcaggatcg caacgatatc gaaatgcttc gcgaaaacat
    44041 ggttaagtcg aaaggccgca ataactttaa aaatctgttt ctctatgccc cacaggggaa
    44101 agccgacggc attaaaatta tcccgctcag tgaagtggca acgaaggacg atttttttaa
    44161 tatcaaaaaa gccagcgccg ctgacctgct ggacgcgcac cgcatcccct ttcagttgat
    44221 ggggggcaag ccggagaacg tcgggtcgct gggtgatatt gagaaagtgg caaaggtctt
    44281 tgtccgcaat gagcttatcc cgttacagga caggatccgc gagataaacg gctggctcgg
    44341 tcaggaggtc atccgcttta aaaactactc actggacact gacaacggct gaacatcgcc
    44401 gcctgcgggc ggctttttta caccccgcca tcacgccctc acacgctcat caccgcacaa
    44461 aaaatcccgc agacacacca acgcccaacg ggcagactaa gcgccgtcac gacgcgctga
    44521 gacgctggaa aataaaatca gcaccaccgc cagcgcgcag tgctttcccc gcctcgcccg
    44581 cccgcttcat ggggcggttt taatgcagtt gcatgaacac tccaagagca cgccagcgct
    44641 agtgataact aacaataaaa gcgacttctg acgcatgcaa atccatgcac atgatggcat
    44701 ttttcatgat ctatgcggta tgctatccgc ttcaattttt aacacttgtt aagggaacta
    44761 gacatggaca ttattgattt aaaatctcaa ctagaaaatg aagataatgc caagaaaaga
    44821 gcagagttgc ttgctcagct agaggtgata aagcaaaacg ctgaagagca aattcgtgct
    44881 gagcaaaagg atgtggattt tgagaccaaa gaatttaccg ttgagttatt agttaataag
    44941 tatcataacg gattagagga tgatactaac gaactctttg ttcctgatta tcaaagggat
    45001 tttgtttgga gtgaaaaaag acaatcaaaa ttgatagagt ctttgatttt gggttttcct
    45061 ataccatata tatttatagc agatgttctc tccgaagatc ccgatttaga tgggcgaatt
    45121 gagattgttg atggctcaca acgagttagg actatacacg cttttatcca aaatgaatta
    45181 gttttacaag acctgaaatc tttagacgtc cttaatggtt tcaaattcca agatttacca
    45241 ttatcacgtc aacgtcgttt catgagaatt cctgtccgcg ttatagaatt gagttctaaa
    45301 tgcaatgaag aaacccgtag agatcttttt gagcgtatta actctggcag cgatatattg
    45361 aaagatatgg aagtccgtaa aggatcagaa cttggttcaa caaatttata tacacaggtt
    45421 attaagccat gctcaacaat acctaaattt aaagaattag cacctttatc tcagactaaa
    45481 gaaaaacgag atgaacggtt agaatttaca ttaagattct ttgcctacgt agataagtac
    45541 aagaactttg atcattccgt acgggatttt ctaaatgact atatgtcaga aaatcgagat
    45601 atagacgaaa ctaaacaaaa ctcaatgaaa caagagtttg ctaatgttct atcttttgtt
    45661 gaaaactatt tcccagcagg attcaagaaa actattactg caaaatctac acctcgagtt
    45721 agattcgaat ctctcgctgt tggcgcagca cttgcattac gtgaagtccc tgaccttgtt
    45781 cccaagaact tagattggct aagctcagaa gaattcaaac gattgaccac ttccgacgga
    45841 gctaatagtc gcgtgaaggt aattgaacga attgaatatg ttcgggataa attattagag
    45901 gattaacatg gatcgttttc gtgaggattt tgacgaaagg tcaggagaga tcttggccta
    45961 tttggatctt ttgaaattta ttgagtatgc cggtgcagaa ctgatatcag ctgatgataa
    46021 agaacataag ttctccatta ctgctcaatc acgaaaaact ctaaagggag cagtatatat
    46081 actgttatat aacctcattg aatcaacaat gagagaagct atctgcctta ttcatgagac
    46141 tatttatgat agaaacgtag aatttgacaa actcaggaaa aatctcaggt cagaaatatt
    46201 aaaaagatta aaaaatgaat ctgtaaatgt tgaaagttta gttaatcacc ttaccaaagg
    46261 tatatcttgc ggtatatctt atggcacgtt caacaaaaag aaaatattct caggaaatat
    46321 tgatagagag gaaatcaaag aaaaatcgaa aatatatggt ttttctacac attccgatta
    46381 cactcacact aaacatggtg aaaaattagc cactgttaaa caacatcgta atgatttatc
    46441 tcatggcaat gtatcatttg ctgagattgg aaaaaatgtt tcatatcagg acttagagaa
    46501 tatttcatta gaagtaatcg cctatcttga tgctatcgtg aataatatag agcactatat
    46561 aaataataat gaatatttag aacaatagtt ataaggcctg taaagcaggc cattttatca
    46621 ttctaaatgg ttcttgattg attgcccaat tgcttttcct agttccacag gaacagcatt
    46681 acctatcatt ttcccaatac ttcttataac aaacttgttt ttgtcttcta caaaaatata
    46741 atccatcggg aaagtttgca ataaagcagc ctctctaaga gaaatcgctc tatgctgttc
    46801 tggatgtcca aaacgcccat tcccgaaacc ataacaaaga gttgtaattg ttggacttgg
    46861 cttatcccaa gacatgcgcc catatacact agcataccct ttaccgctgg atttcatatg
    46921 acaagcggca accaactctt tcggccagtc cttccatgta ccaccaggaa cagagtgtat
    46981 aattcttttt ttattaatcg gatttaattt actggctcta tgtaatatat catttggatc
    47041 tgtttggcca tcttctaatg gaggaagatt tcctattgca tctctaactg ttttcaaaat
    47101 aacatcgtca cgttttacta actcaatcgc ccccaatttt gaggccaaca atacatgacg
    47161 ggaacggttt tgtgggattc catattcgac acaatcaatc ttagacgccc aaacataata
    47221 gcccaacttt aacaatgaat tataaaaatc attataaact ttatgtttag ttacatcagg
    47281 aacgttctcc attgttacga tttctggaga tacttcgcga attaaacgtt cgaactcata
    47341 aagaagaggc cattttttat catcagcctt atcttttcct tgagtatatt ttgaaaaagg
    47401 ttggcaaggt gcgcatcctg ccaatacttt tacagacgca tcaccataaa gcctcagcag
    47461 ttcctctttt gttactttag cgatatcttg ctcaataaac ttagatttat tgtttctttc
    47521 atagggaaac ctacattccc cctcaaggtc aatgcctgca acgacatcaa gccccgcaag
    47581 ctgcaaacca tgagttaatc cgccagcacc acaaaaaagg tctatcacct ttacagtcat
    47641 cactcactcc aaatctatac gatactcagt atacatccct ttctacaaaa tctcgaggcc
    47701 taacttataa actcatattt attgagttaa cgcttcactg agtttgactc taatactgtt
    47761 gagaatcccg accactcatc agcgagcggg taagtgaatt ttttcccgtc ataatttacg
    47821 gttgccccac gcgccagcac ctcaagctcc catcgctgcg gcctgatacc gttctgagca
    47881 aggtcaacgc ggatacgggt aatttgcatt cgttccgacc gggtcagtct ggccgatggc
    47941 gctatttcat gcggttttaa cgggcttccg tttctttgct gacggtttgg tcttctcagt
    48001 ccgtgtttta atgcacctct gagcgccctc acaacctccg ggtcattcca ttcgataaca
    48061 ccgtcatcta ccagattaag cactgctgcg gcgtgctcag aaggtgtggg agccggtaac
    48121 gaagtatcac caccagtgag ctttccacag ttattgacag gactccgagg cgcggcgatg
    48181 ccgcttttta aagtcaaagg ctcaacgacc ggcactttcg gaacaatgcg ccagtccgtc
    48241 gttctggtga tatgaatatg acgcgcgccg agatgcggcg cgtaaatgcc gaccactctc
    48301 tcgacttctt cctcgtactc gttaacttca tccgacggac tacgggcgac cctgacagtc
    48361 tgacaatcgc gcgggacatt tgccccaccc tgcgcgctga tatacagcgc aaaatcacca
    48421 ctgtctgcgg cggcgcgtgc agcctcgacg cgctcgtcaa actcatcagc aatgctgacg
    48481 ccgcgaggca atttgcgtag ttcacggtaa gcccccattg tcggcaggcc aaccgtttta
    48541 aattgcggaa tgcgccacgt tgacgcccat gcggtaacag ccgcggcagt atctttcagc
    48601 ggtctgccgc tatcgttatc gagctgccca tccagtgcat agccgtcgat attttttgag
    48661 atgtatttcg cgatataccc cgcagcaccg ccccgattaa gatgttttgc ctgaaaacgg
    48721 tttcgcgcag ctcctctttc gtcgccatcc tctttgagcg cgtagcgacg catgatttcg
    48781 ataatctggt tacgctggcg tggattacaa aaaagcatca tatgccagtg cggcgttccg
    48841 tcgtggtgtg gctcgacgac tcgcaaaccg tagacctgta aatcattatc cttgaatgcc
    48901 gtgcgcatca ggctccagat acggcagaga taacgctgcg catcctttgg attaaatgcc
    48961 tcatcgttcc agccgtgatt tagctggacg gttttacttt cgccttttcc aacctgacgt
    49021 gtcgggtgat actttgacgg cgcggtcagc gtgataaaca tccccacatc accttctgcg
    49081 gcggcgtaac gctcaatacc ggcaatggtg ttcatcagct ccatccggcg aatttcagga
    49141 ttagaaatac tgcccatcac cttactgata aggtcgatgc gctcgcctgt ttccctgttt
    49201 tcgagatcac acgatttaag aaattccaga tttgcctggc ggcgtgcacg cacatcacga
    49261 atggcatgtt tactggcata aggagaacgg tctttattga cctccccgac agcaatcagt
    49321 aacgcctcat gccagcgcat acgctggcct ttaagctgac taatccacca ctcatcgtta
    49381 aacagacggg caatggcaga atatgcctgc ctcgtggtca tctgtccttt acggtatttt
    49441 ttccagtaga gaggggaaat attgaaagca cgtgcagcgc cagcaacatg accatagagg
    49501 tgagcctgcg cctcatccgt aaacagcgat tctttttcgc catgcgcatc cacccaggca
    49561 tcgctgagtt cctcatacat catgaaaagc tgcgatgaga tacgggcggc aaactttttc
    49621 agctccttgt cattcattcc cggcaggcgc gcatagtggt cacgctcagc cagaaacagc
    49681 atcgacgcgt cggtgttcat ttcatggcgc tgattcacac gctcaatgcg cggccataaa
    49741 cgacgctgaa aagtggatgt gaggaaataa aacccgtgca ccgggctttt attgcgccgg
    49801 atgtagtcat agcgtgacgt aaacagcgag cgcaaaaagt aaggcaggcg gttaatcgtg
    49861 gataaaacac cttgcacctg acgcatctcg tcacgtgtaa ggggtctttc gcgcccgaca
    49921 gcctcgcgtg gcgcgttcca tgcataagca ccggtaaacg ccttaccggt gcctgcagca
    49981 aatgctgacg gagggacaaa acgcccggag gctttaacgg ccatatgagc caaaagcctc
    50041 tgaacaacgc ttgctgagtt gctcaacctg cgcgtttaaa tcagcaaaag actttgcgct
    50101 tccggtcaga atatcgtgat gcatcaggcc ggaaacgagc tggcttaatt tcgggtaata
    50161 accaaccacc gccagccatt cctgaccggc gtttttaccg ctttccgctc tctttttctc
    50221 gtggagaata aactggaagc tgtcactggt aacgacataa cgttcgccaa tttcaatacg
    50281 aatactcatg ccgttctccg gtaatgtttg ttttttgctt caaagactga ctgacaggaa
    50341 acacaacgcg tggctgacgg ataagccgca cgacgggcag caggaattgg cgcgtcacac
    50401 tcttcgcaaa ccagcgcaga agcaccgcaa tgttttaccc ttgccgcgtt aatctggcgc
    50461 tccagtaatt cagcctgttg ttcctgaata aaatctatgt tgtccggcat tatcagctcc
    50521 ttttatcgtt aagtttcctg gatacatcag cgcaataact ggcaagttct gtcgttaatt
    50581 ttgtcagttc atccactgag gaaatttgct tgtggaatac agcgcgttta acaagtaaat
    50641 tgaccacatc agacaggagg tttaattcat tctgataaat cgcgataaca gattcagtga
    50701 tgtcgcgttt ttctttatca agacaaagtt gaataagaga caagtcgcca tttttcataa
    50761 cggcgatttt taaggcgtta ttcagtaata caactgaatg agaacaggac atcaaagcac
    50821 ctccccgcga gacaatccga tattgtgaaa tttttccgac tcctgactga gcagctcgac
    50881 tatctccacg cgggataact ccgcctttgt gatatggcga atcatggcgt caagatgaga
    50941 agaaaagcgc gtcgcagcgt cggcctgtgc ttcggttctg gcctgttgca gcagtaatgc
    51001 gtatttaccg cactgatttt cagaaactgt atgcatgact ttctccaggc aaaaagaagc
    51061 cccgcacaat taagtgcgtt aaaaactctg gttaattatt taatgcaaat attgctctgg
    51121 ttttaccgac gtcagaattg tcggtgcata ctcaaacaga ctgaatagtt cacgtaatgc
    51181 acggaataaa gcatcacgcc agtaacatga ctcttcatta attcgccagt atggctgatt
    51241 gaattctttt tcagtcaatc cggcatgcat aaataaagta cgacgctgac tgactgttaa
    51301 aaaactaata tatgcatact cacttgcgcc aacctgacgg cgttttgaga atgccccacg
    51361 caattcatca attgcacaaa ccagccgttc acgttcgacg tcgttcattt cttcaaaacg
    51421 catcgttgcg tgacgttgtt ttaactgcgc atgaaagcaa actgttagcc gttcgcgctc
    51481 catcatctga ttataataat cacatgtatc ctgccaacga gggacggcaa gatgcttacc
    51541 aattatccgg cgcatagctg ctggctgttt ttcgacgaga ttgagcgtca tcactgtcat
    51601 ttccagaccc tccggctttt cagaaaggtc agagcctttt ttaacggact ctgttttttg
    51661 gtgcggataa tgattccctt gcgacccttc ccgtgggtga tggtgaagtc aatcgcccta
    51721 gggctttcgt tacgcaataa ctgagcaata caacgcggct cattcataat cacaacccca
    51781 tccacaaaag ccatgcatca cgctgttcaa ccggtcggtt ataaaacgcc tcacgtacag
    51841 cgcgattaaa ctcaggaatg aaaacccatt tttcaccggc acgagccttc ggtttgcaag
    51901 gatcacgcaa ttcaataatt ggtaatttat ttgccttcac catttcactg acggctgtct
    51961 ttggcttccc taataaatca gcaaatttat ccacatgaac cgcatcaagc ggatactgaa
    52021 tcacataatt ttcagcgtcc atatatggta ccctcatagg atccagccct ttctaaacca
    52081 ctcaaaaccg tttagacgct ggtttattct caaatcaatg gaacctatat aggttccagt
    52141 tttgagggaa tttagtccct atataggcac catgtcaaat gaaattaagc gaaaagatta
    52201 aggccttgcg tgaggctgaa gggctaagcc aatcaaaatt ctgtgaaatc atagagttac
    52261 cgctaagcac acttaaaaaa tatgaaggag gaaactttga acccggtggc acagctttgc
    52321 taaaaatcac tatgcatccc acattccaaa aatatgctct atggcttatg acagataaaa
    52381 ccgcgccgga cgcaggacaa atcgcaccgg ctctcgcgca cattgggcca gagtcaacag
    52441 agtccaacca ctccgcgaaa aggattggct aactctatat aaagattaca ttttcaccat
    52501 ttgctaccaa gatggtgaat acagcgccgg agggctttct tatggcaatt aagaagctcg
    52561 atgatggtcg ctatgaagtg gacattagac ctcgcggtcg cgacggaaaa cgcatccgca
    52621 ggaaattcga aagaaaagct gaagcactag catttgagcg atacacaatc gccaatgcca
    52681 gtcagaaaga atggggaggc cagcgagcag accgccggac tttgagtgag ttgctggaca
    52741 tctggtggaa atatcacggg caaaaccacg agcatggaac aaaagagttt aatcatctac
    52801 tcaaaaccat cagcggcata ggtgatatac cagtgagcag gatgagcaaa agggctttga
    52861 tggattatcg ttccatgcga ctacgtgatg gtatcagtgc cgcaacgata aaccgtgaca
    52921 tgtacagatt atccggcatg ttcacaaaat taattcaatt ggatgaattt tccgggcaac
    52981 acccaattca cggactgccg ccactggcgg aggccaaccc tgaaatgacg ttcctggaaa
    53041 aagcagaaat cgaaaaactg ttaaatgttt tggctggtga tgacttactt gtcgcgcttt
    53101 tatgtctgag cactggagga agatggacgg aagttgccac gctaaaacca gcacagatta
    53161 caaattgcag ggttaccttc ctgaaaacca aaaacggtaa aaagcgaacc gtgccgattt
    53221 ctgaggaact ggagaaaaaa gttaaagagg aggccagcgc caaattattc aaagttgatt
    53281 atgagaaatt ttgcgggatt ttacgcagag tgaaacctga tataccaccc aatcaggcaa
    53341 cccacatcct gcggcataca ttcgcaagcc atttcatgat gaatgggggc aatataatcg
    53401 cactgcaaca gattctggga catgcgagca ttcagcagac gatggcctat gcgcaccttg
    53461 cgcctgacta tctgcaaaat gccgtcgctc tgaatccact aaaaggcgga gtgacgttat
    53521 aaatttcccc ctctgagtgt ccacatagtg tccacactca agaaattttg aaaccgctcc
    53581 aaacccttac agctttcgat aagtaactgt ttttactttt gattgcatgt aagtatttga
    53641 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    53701 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    53761 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    53821 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    53881 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    53941 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    54001 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    54061 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    54121 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    54181 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    54241 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    54301 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    54361 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    54421 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    54481 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    54541 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    54601 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    54661 tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc gcactggagc
    54721 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    54781 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    54841 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    54901 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    54961 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    55021 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    55081 tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg ttacccaagc
    55141 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    55201 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    55261 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    55321 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    55381 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    55441 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    55501 gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    55561 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    55621 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    55681 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    55741 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    55801 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    55861 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    55921 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    55981 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    56041 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    56101 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    56161 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    56221 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    56281 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    56341 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    56401 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    56461 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    56521 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    56581 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    56641 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    56701 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    56761 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    56821 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    56881 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    56941 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    57001 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    57061 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    57121 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    57181 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    57241 ccatatttat tttatgtcag gcgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    57301 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc aggtttattt
    57361 caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg accagataga
    57421 ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca gcgaagttgc
    57481 tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag gtgtcgctac
    57541 cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac cgccaatcaa
    57601 tttatcggcg ataatcaacg gaataccggt cacgataatt actgccacac ccaacaggat
    57661 ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca gcgcgaaggc
    57721 aaagaatgga atcagcgttt gcactgcttt gctgaaaaat tctcgtaatt cagggtcaag
    57781 attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa catgcggttc
    57841 aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc cggactccaa
    57901 cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca tgatggaagc
    57961 gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg aaagtccggc
    58021 aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg ccgcaaccac
    58081 ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta ccgttcccgt
    58141 cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa tgggcaccgt
    58201 accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg gcgagaaggt
    58261 gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga ttttctcaat
    58321 cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct gatgcgtaat
    58381 tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta atcaataaaa
    58441 gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt atcggatcat tgtttttatt
    58501 ttgaaattaa tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt aagctgatat
    58561 gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc gcaaaacgtg
    58621 ccgctgcttc gtcccagttc accacgttcc agaactcttt aatgtagtcc ggacggcggt
    58681 tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc gggaagccgg
    58741 aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta gaaaccaccg
    58801 ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg gaagctgccg
    58861 ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg atagccgctt
    58921 tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg ctgtggttag
    58981 cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt
    59041 tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc agcgccgcgt
    59101 tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat
    59161 cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta tagctcatat
    59221 tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt ggttcattat
    59281 agttaattaa atgatattga aaatgattat caatgccgta cttttcgtaa gggtatggtt
    59341 ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg attgccagta
    59401 aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt acactcatct
    59461 cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa cgcgattacg
    59521 atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta cgctccgttc
    59581 aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat tgtttcgtgg
    59641 attattctgc cgtggaccat cagcgccctg ttactaccgg atttctgggc gtattacagc
    59701 tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg ggggatcggt
    59761 aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat tggcatcgcc
    59821 attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg caatttcgat
    59881 gtgctgatta acaccgaagg cggacgcatg acgttgctcg gcgttctggt ggcgctgatt
    59941 ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg cattaaagcc
    60001 gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat tttctctgcc
    60061 gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc cgcacttggc
    60121 gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg cggcgcgatc
    60181 attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc gctaaaagcc
    60241 gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc actgggcggg
    60301 ttgatgtggt atctgcaatt ctttttctat gcctggggcc acgcccgcat tccggcgcag
    60361 tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg cggtatcgtc
    60421 gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt gttgagcctc
    60481 ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc gaattaatct
    60541 ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
    60601 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
    60661 cggctatgct ggagaagata ttgcgcatga cacactctga cctgtcgcag atattgattg
    60721 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
    60781 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccaa ccgggtaatc
    60841 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
    60901 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacgac
    60961 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
    61021 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
    61081 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
    61141 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
    61201 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
    61261 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
    61321 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
    61381 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
    61441 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
    61501 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
    61561 ataggcgatg tcagtaacgc tggcctcgct gtggcgtagc agatgtcggg ctttcatcag
    61621 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
    61681 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
    61741 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
    61801 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
    61861 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
    61921 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
    61981 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
    62041 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gagcgcgtac
    62101 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
    62161 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
    62221 cgggagccgg ggttctatcg ccacggacgc gttaccagac ggaaaaaaat ccacactatg
    62281 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
    62341 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
    62401 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaatgaag caaattgtga
    62461 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
    62521 aggtcacgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
    62581 tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg
    62641 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
    62701 gcctgcatag ccagaacggt tatgtcacct ggaatgtgga tagcctggaa agtgccattc
    62761 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
    62821 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
    62881 cttatcgcga tagccgctcc aatggcctaa tggcgcaggc acagcaacaa ctcggcaaac
    62941 gcgatattta tcaacgtagc ggcatccagt ttctgccctt caatacgctt tatcaactgc
    63001 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
    63061 cggattactt cagctatcgc ctgaccggca agatgaactg ggagtacacc aacgccacga
    63121 ccacgcaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
    63181 gggctaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
    63241 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
    63301 cggttatcgc ctcgccgtta aacggttcac gcgctgctta tctctcttct ggcacctggt
    63361 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
    63421 taaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
    63481 ggctgcttca gcgagtgctt caggagcggc aaatcaacga tctcccggcg cttatcgccg
    63541 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta
    63601 atcctgaggc gatgtgcagc gaaattcagg ctgcgtgtcg ggaaatggcg caaccgctcc
    63661 cggaaagtga tgctgaactg gcgcgctgca ttttcgacag tctggcgctg ctgtatgccg
    63721 atgtgttgca tgagctggcg cagctacgcg gtgaagattt ctcgcaactg catattgtcg
    63781 gcggcggctg ccagaacacg ctgctcaacc agctatgtgc cgatgcctgc ggtattcggg
    63841 tgatcgccgg gcctgttgaa gcctcgacgc tcggcaatat cggcatccag ttaatgacgc
    63901 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
    63961 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag cttcactcga
    64021 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
    64081 gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc aacttgatcg
    64141 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
    64201 aggttcgctg accggtggga ttcaggctac tggtaattat ccgggcaaag cgcgtaatgc
    64261 cagtgagcta cgagccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
    64321 taatttacat gccatctatc tggaatcaga cacgccagtc tcgcgcgacc agatcaaacc
    64381 tgagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
    64441 cccctcctgt ttctcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
    64501 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
    64561 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
    64621 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
    64681 cagcgagaag ctggatccgg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
    64741 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
    64801 ccagactgcg ctgtgcctgg acgccgggca tttccacccg actgaagtga tttccgacaa
    64861 gatttccgcc gccatgctgt atgtaccgca gttgctgctg cacgtcagcc gtccggttcg
    64921 ctgggacagc gatcacgtgg tgctgctgga tgatgaaacc caggcgattg ccagtgagat
    64981 tgttcgtcac gatctgttcg accgggtgca tatcggcctc gacttctttg atgcctctat
    65041 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
    65101 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
    65161 tctggcgcta ctggaagaac agaaatcgtt gccgtggcag gcggtctggg aaatgtattg
    65221 ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
    65281 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcgcgagcgc cttatccggc
    65341 ctacaggtcg aatgaccatg cgtgctgcca ccctcacccc ggccctctcc ctgaaagggc
    65401 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
    65461 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
    65521 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
    65581 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aatggtggta
    65641 acctgacgct gcgcctggat gacgccgata tcgcgcctta tcacggcaac tttcatgcac
    65701 aaccgcgcta tattcccctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
    65761 tcaccggctc cggcaaattc ttccgtaacg tccagcttga tcctgcagct aacttaggcg
    65821 tcgtaaaagt cgacagcgac ggcgcgggct accatattct ctgggggtta accaacgaag
    65881 ccgttccaac ttccgaactt ccggctcact tcctttccca ctgcgagcgc attaaagcca
    65941 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    66001 atgtacttga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgagt
    66061 gtctggtggt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    66121 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    66181 acggcgtctt cggcagcggg tcgacgttgg atgaaacatt cggtttaatc gacaccgcag
    66241 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    66301 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gctagtgcgc
    66361 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggtt aaccccgacg
    66421 cccacgaaga gtatcagcgt cggcataacc ccatctggcc ggaactggaa gcagtgttga
    66481 aatctcacgg tgcgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    66541 ccacggtaga gattgaatct gaagaacggt ggaatgcggt agccagcact gatgtttgcc
    66601 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccggataac agcccggtga
    66661 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgcca gagatgcctc
    66721 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
    66781 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
    66841 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
    66901 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
    66961 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
    67021 ttgacctgaa tattcagggc aatggcgctt attccgttct gaagcagttg gcgacaatag
    67081 cgttacaaaa cggttttatc accgactcac accagttcct gcaaaccctg ctgctgcgcg
    67141 aaaaaatgca ctctactgga tttggttccg gtgtcgccgt gccgcacggt aaaagcgcct
    67201 gcgttaaaca accgtttgta ttattcgccc gcaaagcgca ggctattgac tggaaagcca
    67261 gcgatggcga agacgtcaat tgctggatct gcctcggcgt gccgcaaagc ggcgaagagg
    67321 atcaggtcaa aatcatcggc acactgtgtc gcaaaattat ccaccaggat tttattcatc
    67381 aactgcaaca gggcgacgcc gaccaggtgc ttgccttgtt aaatcaaacc ctcagctcat
    67441 aaggaagtgg cgatggagtc atccttatgt attgtcgcga tcaccaactg ccccgccggg
    67501 atcgctcaca cctacatggt ggcggaagcc ctggaacaga aagcccgttc tctcggccat
    67561 accataaaag tggaaactca agggtccagt ggcgttgaaa accgcttatc cagcgaagag
    67621 attgccgctg ccgattacgt cattctcgct accgggcgtg gcctgagcgg tgatgatcgc
    67681 gcgcggtttg ccgggaagaa agtttatgag attgccatct cccaggcgtt gaaaaatatc
    67741 gaccagattt tcagcgaatt accgacaaac tcgcagcttt ttgccgcaga tagcggcgtg
    67801 aagcttggca aacaggaagt gcaaagcggc agcgtgatga gccacctgat ggcgggcgtc
    67861 tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg tggcgcttgc caacatgctg
    67921 gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg cgccgtcgtt tacctgggta
    67981 gttgaatcca tcggttacct tggcttcacc tttatgatcc ccatcatggg cgcttatatt
    68041 gcctcgtcga ttgccgataa accggctttc gctccggcgt ttctggtttg ctatctggcg
    68101 aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat tcctcggcgc ggtggtgctg
    68161 gggctggcga ttggctattt cgtcttctgg ttccgcaaag tgcgccttgg caaagcatta
    68221 caaccgctgc tcggttcaat gctgatcccg ttcgttactc tgctggtttt cggcgtgctc
    68281 acttactacg ttatcgggcc ggtgatgtcc gacctcatgg gcgggctgct ccacttcctg
    68341 aataccattc ctccatcaat gaagtttgcg gcagcgtttc tggtcggtgc gatgctggcg
    68401 ttcgatatgg gcggccccat caataaaacc gcctggttct tctgcttctc actgctggaa
    68461 aaacacattt atgactggta cgccatcgtc ggcgttgtcg cgttgatgcc gcccgttgcg
    68521 gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc gacaggaaaa agaggctgcc
    68581 agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac cggctatacc ttacgctctg
    68641 gcagctccgt tgccgatgat taccgccaat acgctggcgg gtggcattac cggcgtgctg
    68701 gtgatcgctt tcggaatcaa acgtctggca cctggtttgg gtatctttga cccgctgatc
    68761 ggcctgatgt cgccggtggg ttcgttttat ctggtgctag cgattggcct cgcgctgaac
    68821 atttcattca ttatcgtcct gaaaggattg tggttacgcc gcaaagcgaa agccgcgcaa
    68881 caggagcttg tccatgaaca ttgagttact gcaacagttg tgcgaagcca gcgccgtcag
    68941 cggcgatgaa caggaagttc gcgacattct gataaacacg ctggaacctt gcgttaatga
    69001 gatcaccttt gatggtctgg gcagctttgt tgcccgtaag gggaataaag gtccaaaagt
    69061 tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc acccacatcg acgagagcgg
    69121 ttttctgcgc tttaccacca ttggcggctg gtggaatcag tcaatgctca accaccgggt
    69181 aaccatacgc acacacaagg gatttaaaat ccctggtgtg attggttccg tcgcacctca
    69241 tgcgttaacg gaaaagcaaa agcaacagcc gctgtcattt gatgagatgt tcattgatat
    69301 tggcgcgaac agtcgcgatg aggtggaaaa acgcggtgtg gcgatgggcg attttattag
    69361 cccggaagcc aattttgcct gctggggcga agataaagtg gtcggcaagg cgctggataa
    69421 ccgcatcggc tgcgcaatga tggctgaact attgcagacg gtgaataatc ccgaaattac
    69481 gctgtatggc gtcggcagcg tggaagaaga agttgggcta cgcggggcac aaacctcggc
    69541 ggaacacatt aaaccggatg tggtgattgt gctggatacc gctgtcgcag gtgatgttcc
    69601 gggcattgat aacattaaat acccgctgaa actgggccag gggccggggc tgatgctgtt
    69661 tgacaagcgc tacttcccca accagaaact ggtggcggcg ttaaaaagct gtgccgcaca
    69721 taacgattta ccgctgcaat tttccaccat gaaaaccggt gcgacggatg gcgggcgcta
    69781 caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc ctgccaaccc gctatctgca
    69841 cgctaacagc ggcatgattt caaaagccga ttacgatgct ctgctcacgt tgatacggga
    69901 ttttctgacc accttaaccg cggagaaagt caacgcgttt agccagttcc gtcaggtgga
    69961 ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc tggagcaaca gccgcgcacg
    70021 cctggtgagc tggcgcaaca gactggcgtt tcaggcagga ccatcctgcg tgatattgac
    70081 tatctcaact ttacccttaa cggcaaagcc cgcattttcg ccagtggcag tgcgggctat
    70141 cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc tgcaaaagca cgataacgac
    70201 gatcggctgc tggcgctgtt attactgaat acctttactc cccgtgcgca actcgcctcg
    70261 gcgcttaatt tgccagaaac ctgggtagca gagcgtctgc cccggttaaa acagcgttat
    70321 gaacgcactt gttgcctggc cagtcgcccc ggtttgggcc atttcattga tgagacagaa
    70381 gagaaacgcg ttatcttgct ggcgaacttg ctgcgcaaag atccgttttt aattccgctg
    70441 gcgggcgtaa cacgagacaa ttttcagcat ttatccacgg cctgcgacaa ccaacaccgc
    70501 tggccgctca tgcagggtga ttatctctcc agcctgattc tggcgattta cgccctgcgt
    70561 aatcaactga ccgatgagtg gccgcaatat cccggtaacg agataaaaca aatcgttgaa
    70621 cagagcggca tgtttcttgg tgataacgct gtaagaaccc tgacaggttt gatagagaaa
    70681 cagcatcagc aagcgcaggt aatttcagcc gatcatgtgc tggggttgct gcaaagggtg
    70741 ccaggcatcg cgtcattgaa tattattgat acgcagctgg ttgagaatat taccgggcat
    70801 ttattacgtt gccttgccgc accggtgtgg attgccgagc accgccaaag cagcatgaat
    70861 aacctgaaag ccgcctggcc tgcggccttt gatatgagtc tgcactttat tacgctactg
    70921 cgtgaacagc tcgatattcc ccttttcgat agcgatctga tcggtttgta ttttgcctgt
    70981 gcgctggaac ggcatcaaaa cgaacgccag ccgatcattt tgctctcgga ccagaacgcg
    71041 attgccacta ttaatcagct cgccattgag cgcgatgttt tacattgtcg ggtgattatt
    71101 gcccgtagct taagcgagct tattgccatt cgcgaagaga ttgagccgtt attgatcatt
    71161 aacaacagcc attatttact ggatgacgcg gtaaataatt acatcaccgt aaaaaatatc
    71221 attacggctg ccgatatcga acaaataaaa cactttctgg cgacggcatt tattcgccaa
    71281 cagccggaac gctttttctc tgcccccgga agttttcatt attcgaatgt acgcggtgaa
    71341 agctggcaac atattacccg gcaaatttgt gcgcaattag tggcacaaca ccatattacc
    71401 gccgatgaag cgcaacggat catcgcccgc gaaggcgaag gtgaaaacct gattgttaat
    71461 cgcctcgcca tcccacattg ctggagcgaa caggagcgac gttttcgtgg attttttatt
    71521 accctcgccc aaccagttga ggtgaataac gaagtcatta accatgtctt gatcgcctgc
    71581 gccgccgccg atgcgcgtca tgagctaaaa atatttagct atctggcaag cgtattgtgt
    71641 cagcatccgg cagaagttat tgccgggtta acaggatatg aggcatttat ggagttactt
    71701 cacaaggggt gaactgtgtc ggatgcgccg catccgacac tgcatttatc atcaaaaacg
    71761 tttaacgcag gctattatag tcatcaacca tttcgttgta ctcgcgtaac gctcgtggaa
    71821 atgagtcttc gaccatccag cttgtgtttg catcctgaag atgctcttta tcccagtcag
    71881 agtacgggac tttatctctg acgcggcgaa catatttttt agtctcaagt tgatactggt
    71941 ctgccgaatt aatgaacgag aaattcatac cgcctttatc cgcttctttg gcctgcgcta
    72001 ccagcgtttc cagttcagag actttcttca ttgccgcatc aacatcaaac ttctcttgcg
    72061 aaatcaggtt attaatttgt ttcgctgaaa tcatgactgc cagagagtag tagtggaatg
    72121 ttttcccttc tctttcttca atatttttca gttccgcaag ctggcgctta tcattaattt
    72181 cctgaatcgc cgcatgataa gattctgcta caggttcaaa ggattccaga ttttttaaga
    72241 atgtctggtg cagcgttttc cctttcgcaa acgcatcgtc tttataattt tcctgggtgt
    72301 aatatttatc catttcgtta atggtattac ccaacgccac ggcagcatca atataactca
    72361 ccgccacgcc atcaataggt tgcagcgcag gcgttaacgc cacagcactt tttacgcctt
    72421 tttcacactc agcgaggttg gattcactaa tgccgtagat gccataaaca gtactctctt
    72481 taccggtagg cccctgttta aaatctttca gccagtcagc ataacgcgcc agactgttct
    72541 gtacagggat ctgtaacttg ttgtagcact tgatatagac atccattttt tcgtcgaaaa
    72601 ccgcttgttc atcgaccacc ggttgcgcag gtgtttcagg tttagcttct gcttttgcaa
    72661 cgggagcttc aggagctggt gcggcaggtt gactattggc aggcgggggc gtttctgttt
    72721 cggctttttt atcatcacaa cccgtcagac cgagggccat gatggcgact ataatagcgg
    72781 aagataataa attccttttc atttaattgc cttataaata tatagataag agagtgaaca
    72841 taataaatga aaaaagaaaa cgtcactaca cgcgttttat ttataaataa aataataaaa
    72901 ttcacgaaat gaataagtcc tgcaatttaa tatatatcgc aggacttatt ttaattaatt
    72961 gctcaaacgc tgcggatggg tataaaccgt tgcccgaccc ggtttacaaa aacctaccag
    73021 tgtcagatta cagcgctcag ccacttctac agcaagcgtg gtcgcggcag acaccgcaaa
    73081 caaaatctct acgccgcaca tcgccgactt ttgcaccatt tcataactgg cacgactgga
    73141 aaccagtacc gcaccttgct gccagctttc cccttcttgt gaccggcggc ctaacagttt
    73201 gtccagcgcc acatggcgac ccacgtcttc atgcccgccg accagttcgc cagatggcaa
    73261 catccaggca gcggcgtgag tacaaccagt cagttgcccc actggctgaa aatcgttgag
    73321 atgacgtaat gcatcatcca gtttgttgag atcaaacgtc tgggtgaacg gtagcggctg
    73381 caccggtttt ccgatgtcat taagttgctc cacgccgcat acgccacagc ccgtacgtcc
    73441 cgccagcgcc cggcggcgct ccttcaaccc cataaagcgg cggctggaaa gctcaatttg
    73501 tacttcaaga ccattacagg aaggaacgac atccatgccg aagatatcgc gcggactttc
    73561 tattatcccc tcggaaagcg aaaaaccgag cgcaaagtac tcaaggtctt tgggcgacgc
    73621 catcatcacc acatgcgaaa tgccgttgta gaccagcgca acgggaactt cttccgcgac
    73681 ctcatctaac cgtgggtgtt gtaaatcatc acgccgccat aactcaattt gacgaacacc
    73741 tgtgatattt gtcacatttt cgatttcttt tcgctgtgtt tttttcactt tctcttaacc
    73801 agataagaac acacacacca acattatggt attctgttac aaacccttcc tggatggagg
    73861 gaaattgagc caattctgga cctttgcggc cccttccgca aagaaaaata actcccactc
    73921 cctgcacacg cagcaagcga atgtaaatgg gacgtgacaa tgtcgaaaca aggagcaatc
    73981 catgcaggtc agcagaaggc agttctttaa gatctgcgct ggcggtatgg caggcaccac
    74041 ggcagcggca ctgggttttg cacccagcgt agcactcgcg gaaacccggc agtataaact
    74101 gctgcgcacc cgcgaaaccc gtaatacctg cacctattgt tccgtaggct gtgggctgtt
    74161 gatgtacagc ctcggtgacg gagcaaaaaa cgccaaagca tctatcttcc atatcgaagg
    74221 cgatccggat cacccggtca accgcggtgc actttgtccg aaaggcgctg gcctggtgga
    74281 tttcatccac tccgaaagcc gtctgaagtt tccggaatac cgtgcgccag gttctgataa
    74341 atggcagcaa atcagttggg aagaggcgtt tgatcgcatc gccaaactga tgaaagaaga
    74401 ccgcgatgct aactacattg cgcaaaacgc cgaaggcgtg actgttaacc gctggctctc
    74461 caccgggatg ctgtgtgctt ccgcgtcgag taacgaaacc ggctatttaa cgcaaaaatt
    74521 ctcccgcgcg ctgggtatgc tcgcggtcga caaccaggcg cgtgtctgac acggaccaac
    74581 ggtagcaagt cttgctccaa catttggtcg cggtgcgatg accaaccact gggtcgacat
    74641 caagaacgcc aacctcgtcg tggtgatggg cggtaacgcc gctgaagctc acccggtcgg
    74701 gttccgctgg gcgatggaag ccaaaattca caatggcgcg aagctgattg tgatcgatcc
    74761 tcgctttacg cgtacggcgg cggtggctga ctactatgcc cctattcgtt ccggtactga
    74821 cattgctttc ctgtcaggcg tattgctgta cctgctgaac aatgaaaaat tcaaccgcga
    74881 atacaccgaa gcctatacca acgccagcct gatcgtgcgt gaggattacg gctttgaaga
    74941 tggcctgttc accggctatg acgcggaaaa acgcaagtac gataaatcct cctggactta
    75001 cgaactggac gaaaacggct tcgccaaacg cgataccacg ctgcaacatc cgcgctgcgt
    75061 gtggaacttg ctgaaacagc acgtttcccg ctacacgcca gatgtggttg aaaacatctg
    75121 tggtacgcca aaagacgcgt tcctgaaagt ctgcgaatac atcgcagaaa ccagtgctca
    75181 cgataaaact gcctcgttcc tgtatgccct cggctggacg caacactccg ttggtgcgca
    75241 aaacattcgt acgatggcga tgatccagct gctgctcggc aatatgggga tggcaggcgg
    75301 cggcgttaac gccctgcgcg gtcactccaa tattcagggg ctgacggacc tggggctgct
    75361 gtcgcagagc ctgccaggtt acatgacgct gccaagcgag aagcagaccg atctgcaaac
    75421 ctaccttacc gccaacacgc caaaaccgct gctggaaggc caggtaaact actggggcaa
    75481 ctacccgaaa ttcttcgtct ctatgatgaa ggccttcttt ggtgataaag cgacggcgga
    75541 aaatagctgg ggctttgact ggttgccgaa gtgggataaa ggctacgacg tcctgcaata
    75601 cttcgagatg atgaaagagg gcaaggtcaa tggctatatc tgccagggct ttaaccctgt
    75661 tgcctcattc ccgaacaaaa acaaagtgat cggttgtctg tcgaaactga agttcctcgt
    75721 caccatcgac ccgctgaaca ctgaaacctc taacttctgg cagaaccacg gtgagctgaa
    75781 cgaagttgac tcgtcgaaga tccagaccga agtgttccgt ctgccatcga cctgcttcgc
    75841 agaagagaac ggttcaatcg ttaactcagg tcgctggttg cagtggcact ggaaaggtgc
    75901 ggacgcccca gggattgcgc tgactgatgg cgagatcctc tccggtatct tcctgcgctt
    75961 gcgcaagatg tatgccgaac agggtggcgc aaacccggat caggtgctga acatgacctg
    76021 gaactacgcc attccgcatg agccgaagtc ggaagaagtg gcgatggaga gcaacggtaa
    76081 ggcgctggcc gatattaccg atccggcaac cggtgcggtt atcgtcaaga aaggccaaca
    76141 acttagctcg ttcgcccaat tgcgcgatga cggtacaacc tcctgtggct gctggatttt
    76201 cgccggtagc tggacgccgg aaggcaacca gatggcgcgt cgtgataacg ccgatccgtc
    76261 tggcctcggt aacacgctgg gctgggcatg ggcatggccg cttaaccgcc gcattctgta
    76321 taaccgcgcc tccgcagatc cgcagggtaa cccgtgggat ccgaagcgtc agttgctgaa
    76381 atgggacggc actaagtgga ccggctggga tattccggac tacagcgcag cgcctccggg
    76441 cagcggcgtc gggccgttta tcatgcagca ggaaggcatg gggcgtctgt ttgccctcga
    76501 taagatggcg gaaggtccgt tcccggaaca ctacgagccg tttgaaacgc cgctgggaac
    76561 taacccgctg catccaaacg ttatctcgaa cccggctgcg cgaatcttta aagacgacgc
    76621 cgaagcattg ggtaaagccg ataagttccc gtatgtcgga accacctatc gtctgaccga
    76681 gcacttccac tactggacca aacacgcgct gttgaacgcg attttgcaac cagagcagtt
    76741 tgtggaaatc ggggagtcgc tggcgaataa acttggcatt gcccagggcg ataccgtgaa
    76801 agtctcctcc aaccgtggct atatcaaagc caaagcggtg gtgaccaaac gtattcgcac
    76861 gctgaaagcg aacggcaaag atatcgatac catcggtatt ccgattcact ggggctatga
    76921 aggcgtagct aaaaaaggct ttattgccaa tacgttaacg ccattcgttg gtgatgcgaa
    76981 cacgcagacg ccggagttta agtccttcct tgtgaatgtg gaaaaggtgt aacggagacg
    77041 acatatggct tatcaatcgc aagatatcat tcgtcgttcc gcgactaacg gtctgacccc
    77101 cgcgcctcag gcgcgggact tccaggaaga agtggcgaaa ctcatcgacg tcaccacctg
    77161 tatcggctgt aaagcctgtc aggtggcgtg ttcagagtgg aacgacattc gcgataccgt
    77221 cggcaataac attggggtgt acgacaaccc caatgattta agcgccaaat cgtggacggt
    77281 gatgcgcttc tcggaagtgg agcagaacga caaactggaa tggctgatcc gcaaagacgg
    77341 ctgtatgcac tgttccgatc caggctgcct gaaagcgtgc ccggcggaag gtgcaattat
    77401 tcagtatgcc aacggtatcg tcgacttcca gtccgagcag tgcattggct gcggttattg
    77461 cattgcgggc tgtccgttcg acattccgcg cctcaacccg gaagacaacc gcgtctacaa
    77521 atgtacgctg tgcgttgacc gcgtggtggt tgggcaagaa ccagcctgcg tgaagacctg
    77581 cccaacgggc gcgattcact tcggtacgaa agagtcgatg aaaacgctgg cgagcgagcg
    77641 cgttgctgag ctgaaaaccc gcggttacga caatgcgggt ctgtacgatc cggcaggcgt
    77701 cggtggtaca cacgtcatgt acgtgctgca ccatgctgac aagccaaatc tgtatcatgg
    77761 cttgccggag aacccggaaa tcagcgaaac cgtgaaattc tggaaaggca tctggaaacc
    77821 gctcgcggct gttggctttg cggctacctt tgcggccagt atcttccact acgtgggtgt
    77881 cggtccgaac cgtgcggatg aggaagagaa taatctgcac gaagagaaag acgaggagcg
    77941 caaatgaaac gacgtgacac catcgtgcgc tacaccgcgc cggaacgtat caaccactgg
    78001 atcaccgcct tctgcttcat cctggcggcg gtgagcgggc tgggcttttt gttcccgtcc
    78061 ttcaactggt tgatgcaaat catgggcaca ccgcagctgg cgcgaattct gcacccgttt
    78121 gtcggcgtgg ttatgtttgc ctcgttcatc atcatgtttt tccgttactg gcatcacaac
    78181 ctaatcaatc gggatgatat cttttgggcg aagaatattc gtaagatcgt cgtcaacgag
    78241 gaagtaggtg acaccgggcg ttataacttc ggtcagaaat gcgttttctg ggcggcgatt
    78301 attttcctgg ttctgctgct ggtgagcggc gtgattatct ggcgtcctta ttttgcgcct
    78361 gctttctcaa tcccggtgat ccgattcgcg ttaatgctgc attcatttgc cgcagtggcg
    78421 ttaattgtgg ttatcatggt gcatatctac gccgcccttt gggtgaaagg cacgattacc
    78481 gccatggtgg aaggatgggt taccagcgcc tgggcgaaga aacatcaccc gcgctggtac
    78541 cgtgaggtcc gcaagacaac ggaaaagaaa gctgaatgag tattcgcata atcccgcaag
    78601 atgagctggg ttcgagcgag aaacgtacgg cggatatgat tccgccgtta ttgttccctc
    78661 ggctcaagaa tttatacaac cgccgcgccg agcgtctgcg cgagctggca gaaaataatc
    78721 cgctgggtga ttacctgcgc tttgctgcgc ttatcgccca cgcccaggaa gtggtgctgt
    78781 acgaccatcc gctggagatg gatctgaccg cgcgcattaa agaagccagc gcacagggca
    78841 agcccccgct ggatattcac gttctgccgc gtgataagca ctggcaaaag ctgctgatgg
    78901 cgctaattgc tgagctgaaa cctgaaatga gcggcccggc gctggcagtg attgagaatc
    78961 tggagaaggc atcgactcag gagctggaag atatggccag cgcgctgttt gcctctgatt
    79021 tctcgtccgt cagcagcgat aaagcgccgt ttatctgggc tgcgctgtcg ctctactggg
    79081 cgcagatggc caatctgatc cccggcaaag cccgcgctga atacggcgaa caacgtcaat
    79141 attgcccggt atgtggctct atgccggtgt ccagcatggt gcaaattggc accactcagg
    79201 gtctgcgtta cctgcactgc aacctgtgtg aaaccgaatg gcacgtagtg cgcgtaaaat
    79261 gcagcaactg cgagcagagc ggcaaactgc attactggtc gctggatgac gaacaggccg
    79321 cgattaaagc cgaaagctgc gatgactgcg gcacttacct gaagattctt tatcaggaaa
    79381 aagaaccgaa agttgaagcc gtggcagatg acctcgcctc tctggtactg gacgcgcgaa
    79441 tggagcaaga aggctacgcc cgtagttcca ttaacccgtt cctgtttccg ggtgaaggag
    79501 agtaatctct gacgttttcc gggtggcgca ggtcgcccgg attacagctc atagggtgtg
    79561 acgacaccat ctctcgtatt ccgcgttacc cattcaagat catgccgcaa ttgtctgttt
    79621 gcctcgttct tccctccaac acatcgataa ggttatgggt atgtcactga tgacaagcgt
    79681 atgattgcct gatgcgctgc gcttatcagg cctaccaata aacttcaata tattgaattt
    79741 gcatgacttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac aacgcgcacg
    79801 ttgtcagcaa tctgattgac atcatttttt cccattacca ttcctcgcgc agcttacgct
    79861 gatattcgac accatcttcc tcacaccctt gccagatgcc aggaacactg gttcttgctg
    79921 tttgcgattg atttatcagg tattgatcta ccgcttccct taataggtcc gcacgaggaa
    79981 gattacgctg cacctcgaga tcatcaagtt gcttaatcac ctcattcgat aaatcgagta
    80041 aaattctgcc catatctata cctgccagag agttcataaa tatctcgcca atatcatttt
    80101 tgaatctatg gagagaaaag tacccttgcc gaatctttaa agaaagctca tttacgcatc
    80161 attttttatt tttgcgaggc gttttccaga aataataaga gcgttgcagt tgtttcattt
    80221 attactcaaa aatggtttag ttatcgccca ggatgagtta tgggtgctat gaatatatta
    80281 tattcaggga tatttatttg cataatctga atggtgtagc cgaaaaacgt cagcatccta
    80341 acagcaccag gatttaaggt gaaattaatc tttcatcaca gaccggtcag tactgccacc
    80401 aaccggtctt tacttgctca tcgttataat tttgttggta acagttcggg aaagttgttt
    80461 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
    80521 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
    80581 agtaaatgaa tgatactgac aatgatgcca gcccattatc tttgcataat gtttgtgagc
    80641 tatagatttt aaaaatattt atgagaaaag tatggcacca gaggaggttt tgactatcaa
    80701 aaatcgctca aacctgaact aagatgatga gttaagcatt atctgctata attgccactc
    80761 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
    80821 caacgaagaa taaaaattta tagagttctt attcaatatt accggcatag acagggaatg
    80881 atgtaaat
//
LOCUS       DAQSWQ010000003        79579 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.003, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000003 DAQSWQ010000000
VERSION     DAQSWQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.003"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="R8G18_000177"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="R8G18_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="R8G18_000178"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="R8G18_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HEE9793989.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="R8G18_000179"
     CDS             2354..2758
                     /locus_tag="R8G18_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HEE9793990.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="R8G18_000180"
     CDS             2811..2921
                     /locus_tag="R8G18_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9793991.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="R8G18_000181"
     CDS             3157..3201
                     /locus_tag="R8G18_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9793992.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="R8G18_000182"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="R8G18_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HEE9793993.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="R8G18_000183"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="R8G18_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEE9793994.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="R8G18_000184"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="R8G18_000184"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HEE9793995.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="R8G18_000185"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="R8G18_000185"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HEE9793996.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="R8G18_000186"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="R8G18_000186"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEE9793997.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="R8G18_000187"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="R8G18_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HEE9793998.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="R8G18_000188"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="R8G18_000188"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEE9793999.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="R8G18_000189"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="R8G18_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HEE9794000.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="R8G18_000190"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="R8G18_000190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HEE9794001.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="R8G18_000191"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="R8G18_000191"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HEE9794002.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="R8G18_000192"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="R8G18_000192"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEE9794003.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="R8G18_000193"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="R8G18_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HEE9794004.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="R8G18_000194"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="R8G18_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HEE9794005.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="R8G18_000195"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="R8G18_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HEE9794006.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="R8G18_000196"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="R8G18_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HEE9794007.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="R8G18_000197"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="R8G18_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEE9794008.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="R8G18_000198"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="R8G18_000198"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HEE9794009.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="R8G18_000199"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="R8G18_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HEE9794010.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="R8G18_000200"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="R8G18_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEE9794011.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="R8G18_000201"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="R8G18_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HEE9794012.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="R8G18_000202"
     CDS             23801..24874
                     /locus_tag="R8G18_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEE9794013.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25002..28448
                     /gene="mfd"
                     /locus_tag="R8G18_000203"
     CDS             25002..28448
                     /gene="mfd"
                     /locus_tag="R8G18_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEE9794014.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28592..29548
                     /gene="ldtC"
                     /locus_tag="R8G18_000204"
                     /pseudo
     CDS             28592..29548
                     /gene="ldtC"
                     /locus_tag="R8G18_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="R8G18_000205"
     CDS             complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="R8G18_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HEE9794015.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30129..30761
                     /gene="comR"
                     /locus_tag="R8G18_000206"
     CDS             30129..30761
                     /gene="comR"
                     /locus_tag="R8G18_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HEE9794016.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30823..31362)
                     /locus_tag="R8G18_000207"
     CDS             complement(30823..31362)
                     /locus_tag="R8G18_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEE9794017.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="R8G18_000208"
     CDS             complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="R8G18_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HEE9794018.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33301..33843)
                     /locus_tag="R8G18_000209"
     CDS             complement(33301..33843)
                     /locus_tag="R8G18_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEE9794019.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="R8G18_000210"
     CDS             complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="R8G18_000210"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEE9794020.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="R8G18_000211"
     CDS             complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="R8G18_000211"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HEE9794021.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="R8G18_000212"
     CDS             complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="R8G18_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEE9794022.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36362..36739)
                     /locus_tag="R8G18_000213"
     CDS             complement(36362..36739)
                     /locus_tag="R8G18_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HEE9794023.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="R8G18_000214"
     CDS             complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="R8G18_000214"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HEE9794024.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37435..39624
                     /gene="fhuE"
                     /locus_tag="R8G18_000215"
     CDS             37435..39624
                     /gene="fhuE"
                     /locus_tag="R8G18_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HEE9794025.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPSFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="R8G18_000216"
     CDS             complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="R8G18_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HEE9794026.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41412..42209)
                     /locus_tag="R8G18_000217"
     CDS             complement(41412..42209)
                     /locus_tag="R8G18_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEE9794027.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42220..43224)
                     /gene="holB"
                     /locus_tag="R8G18_000218"
     CDS             complement(42220..43224)
                     /gene="holB"
                     /locus_tag="R8G18_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEE9794028.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43221..43862)
                     /locus_tag="R8G18_000219"
     CDS             complement(43221..43862)
                     /locus_tag="R8G18_000219"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEE9794029.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="R8G18_000220"
     CDS             complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="R8G18_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HEE9794030.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="R8G18_000221"
     CDS             complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="R8G18_000221"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEE9794031.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="R8G18_000222"
     CDS             complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="R8G18_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEE9794032.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="R8G18_000223"
     CDS             complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="R8G18_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEE9794033.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="R8G18_000224"
     CDS             complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="R8G18_000224"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEE9794034.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="R8G18_000225"
     CDS             complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="R8G18_000225"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEE9794035.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="R8G18_000226"
     CDS             complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="R8G18_000226"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEE9794036.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="R8G18_000227"
     CDS             complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="R8G18_000227"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEE9794037.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="R8G18_000228"
     CDS             complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="R8G18_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEE9794038.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="R8G18_000229"
     CDS             complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="R8G18_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HEE9794039.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52391..52975
                     /gene="yceF"
                     /locus_tag="R8G18_000230"
     CDS             52391..52975
                     /gene="yceF"
                     /locus_tag="R8G18_000230"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HEE9794040.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="R8G18_000231"
     CDS             complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="R8G18_000231"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HEE9794041.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54619..57804
                     /gene="rne"
                     /locus_tag="R8G18_000232"
     CDS             54619..57804
                     /gene="rne"
                     /locus_tag="R8G18_000232"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HEE9794042.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="R8G18_000233"
     CDS             complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="R8G18_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEE9794043.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="R8G18_000234"
     CDS             complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="R8G18_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEE9794044.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="R8G18_000235"
     CDS             complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="R8G18_000235"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEE9794045.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="R8G18_000236"
     CDS             complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="R8G18_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEE9794046.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="R8G18_000237"
     CDS             complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="R8G18_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEE9794047.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="R8G18_000238"
                     /pseudo
     CDS             complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="R8G18_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="R8G18_000239"
     CDS             complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="R8G18_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEE9794048.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="R8G18_000240"
     CDS             complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="R8G18_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEE9794049.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="R8G18_000241"
     CDS             complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="R8G18_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEE9794050.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="R8G18_000242"
     CDS             complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="R8G18_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEE9794051.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="R8G18_000243"
     CDS             complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="R8G18_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEE9794052.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68109..68768
                     /gene="flgA"
                     /locus_tag="R8G18_000244"
     CDS             68109..68768
                     /gene="flgA"
                     /locus_tag="R8G18_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HEE9794053.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68844..69137
                     /gene="flgM"
                     /locus_tag="R8G18_000245"
     CDS             68844..69137
                     /gene="flgM"
                     /locus_tag="R8G18_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HEE9794054.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            69142..69558
                     /gene="flgN"
                     /locus_tag="R8G18_000246"
     CDS             69142..69558
                     /gene="flgN"
                     /locus_tag="R8G18_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HEE9794055.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="R8G18_000247"
     CDS             complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="R8G18_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEE9794056.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71243..72166)
                     /locus_tag="R8G18_000248"
     CDS             complement(71243..72166)
                     /locus_tag="R8G18_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEE9794057.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72168..72815)
                     /locus_tag="R8G18_000249"
     CDS             complement(72168..72815)
                     /locus_tag="R8G18_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEE9794058.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="R8G18_000250"
     CDS             complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="R8G18_000250"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HEE9794059.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73646..74854
                     /gene="mdtH"
                     /locus_tag="R8G18_000251"
     CDS             73646..74854
                     /gene="mdtH"
                     /locus_tag="R8G18_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HEE9794060.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74918..75565
                     /gene="grxB"
                     /locus_tag="R8G18_000252"
     CDS             74918..75565
                     /gene="grxB"
                     /locus_tag="R8G18_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HEE9794061.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75699..76259
                     /locus_tag="R8G18_000253"
     CDS             75699..76259
                     /locus_tag="R8G18_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEE9794062.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76365..77411
                     /gene="pyrC"
                     /locus_tag="R8G18_000254"
     CDS             76365..77411
                     /gene="pyrC"
                     /locus_tag="R8G18_000254"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEE9794063.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77485..77730
                     /gene="dinI"
                     /locus_tag="R8G18_000255"
     CDS             77485..77730
                     /gene="dinI"
                     /locus_tag="R8G18_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HEE9794064.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78020..78274
                     /gene="bssS"
                     /locus_tag="R8G18_000256"
     CDS             78020..78274
                     /gene="bssS"
                     /locus_tag="R8G18_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HEE9794065.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78389..79507
                     /gene="solA"
                     /locus_tag="R8G18_000257"
     CDS             78389..79507
                     /gene="solA"
                     /locus_tag="R8G18_000257"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEE9794066.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19593 a        20914 c        19717 g        19355 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aatgatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgatagcgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttggggatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaagtgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gcaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    25021 atacactgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    25081 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    25141 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    25201 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    25261 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga
    25321 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    25381 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    25441 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    25501 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    25561 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    25621 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    25681 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    25741 aagtgagtaa aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg
    25801 aaccactgcc gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    25861 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    25921 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    25981 tcttctcaga gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa
    26041 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac
    26101 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    26161 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    26221 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    26281 ttggcgctgc cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa
    26341 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    26401 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    26461 agcacggtgt cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg
    26521 agtatttgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    26581 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    26641 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    26701 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    26761 gtgagcagta tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    26821 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    26881 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    26941 ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg
    27001 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    27061 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    27121 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    27181 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    27241 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa
    27301 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    27361 aaacctttgt ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt
    27421 tgcgcggggg gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg
    27481 aacggctggc agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg
    27541 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    27601 gtacaaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    27661 gcgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc
    27721 atcatcaggc atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac
    27781 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    27841 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    27901 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    27961 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    28021 ggatgccgtc gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct
    28081 ataaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    28141 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    28201 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    28261 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    28321 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    28381 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    28441 tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    28501 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    28561 acaataacct tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg
    28621 gctaacgttt tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc
    28681 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    28741 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    28801 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    28861 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    28921 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    28981 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    29041 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    29101 gcctacggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    29161 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    29221 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    29281 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    29341 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    29401 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    29461 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    29521 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    29581 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    29641 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    29701 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    29761 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    29821 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    29881 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    29941 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    30001 attaatttaa aaatctttaa atttcagtta aatagaaagc aattattttt atgggatctg
    30061 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggttca
    30121 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    30181 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    30241 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
    30301 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    30361 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    30421 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    30481 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga
    30541 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    30601 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    30661 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    30721 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc
    30781 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    30841 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    30901 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    30961 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
    31021 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc
    31081 tttaccacga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
    31141 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg
    31201 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
    31261 gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc
    31321 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt
    31381 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
    31441 cgcgggaatg gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga
    31501 cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt
    31561 ttatgccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc
    31621 ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct
    31681 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
    31741 agccgcgcgt caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca
    31801 gcgaaccatg atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa
    31861 tattgttcat tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc
    31921 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg
    31981 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
    32041 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
    32101 tatattcacc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
    32161 tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg
    32221 gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg
    32281 tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc attgtgcaat tcagcggaga
    32341 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca
    32401 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
    32461 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg
    32521 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt
    32581 cacctttctc atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
    32641 tgacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
    32701 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat
    32761 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
    32821 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
    32881 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa
    32941 acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata
    33001 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
    33061 ataggcgact ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag
    33121 tccggaaggg tgatataggg tgacgcgtat atcgagggcg tttggtcgtc ctgataagat
    33181 gcgcagcatc gcatcaggca ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc
    33241 gacttacaga attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat
    33301 ttacccgagg gttttgaacg ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt
    33361 gtgcgtctgc tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg
    33421 ctggctgtta agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt
    33481 ctcgcggaag ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat
    33541 cttgccttcc atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc
    33601 gcaaagaaaa ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg
    33661 acgctcgtcg acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc
    33721 atgtttcgga tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa
    33781 ttgtaagact ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat
    33841 catcgcttcc tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat
    33901 gtaactgatt cagacgggtg ctgatcgcct tccagcgagc cgagtccatc agttcctgtc
    33961 gcgaaaatga acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat
    34021 tatctaacac gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacctg
    34081 cgtccagtga tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg
    34141 ataaatcgtc agagaaaatt acaccgtcaa aacacagttc ctgacgcaaa acggttttca
    34201 gccagtagga agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag
    34261 gcataatggc gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag
    34321 cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta
    34381 ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat
    34441 caataaaccg gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc
    34501 caatcgccgc gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca
    34561 tagcgatcat ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt
    34621 ccattcccaa cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa
    34681 aacgctgcac gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg
    34741 cgcggatctg gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga
    34801 gaatcagccc tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt
    34861 aaccttcgac atccaacatt actggaccca cactgctctc cttattgttt tattaatagc
    34921 tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccgatttg tcgccagcgg
    34981 tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc
    35041 cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc
    35101 tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca
    35161 tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca
    35221 tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt
    35281 aacattcgca gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc
    35341 agtaagggca acagcgttat tcgccagcca aaacgtggtt gttgatgaag ataatacagc
    35401 aagcctgcca gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg
    35461 actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt
    35521 gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga
    35581 cgcacaacaa aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta
    35641 ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg
    35701 atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac
    35761 cagcatcagc tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag
    35821 cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact
    35881 gtcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag
    35941 ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt
    36001 cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac
    36061 cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg
    36121 gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg
    36181 cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc
    36241 cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag
    36301 aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc
    36361 cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
    36421 gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct
    36481 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
    36541 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
    36601 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
    36661 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc
    36721 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
    36781 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt
    36841 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
    36901 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat
    36961 gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
    37021 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
    37081 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
    37141 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct
    37201 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc
    37261 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
    37321 atgcgcatac ttctcatttg catttacaaa caaaattatt cgcacactaa atataaccgc
    37381 tcatcaccct cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt
    37441 tcaacacaat ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc
    37501 ggttgcatag cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa
    37561 acggtgattg ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta
    37621 acgtctacct ctgcgggtac caaaatgcag atgactcaac ttgatattcc tcagtcggtc
    37681 actattgtta gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg
    37741 gaaaacacgc tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc
    37801 cgtggattcc agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc
    37861 tggaatctgg gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt
    37921 ggcgcgacag gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga
    37981 aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac
    38041 aaagaacggt atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg
    38101 cgaattgtcg gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag
    38161 accttcttct cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt
    38221 tacgaatatc agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat
    38281 actgatggca gcagcaacag ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac
    38341 aacgataaag agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctgg
    38401 caagcgacac tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc
    38461 gatgcctatg taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct
    38521 agctttgatt atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg
    38581 gatttgttcg ctgacggtag ttatgaattg tttggtcgtc agcacaatct agtgtttggt
    38641 ggcagttaca gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat
    38701 gaaattggca gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag
    38761 agcctggcac aggacgatac cacacatatg aaatcgttat atgctgctac tcgagtcacc
    38821 cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg
    38881 ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc
    38941 aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt
    39001 gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa
    39061 tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat
    39121 aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa
    39181 gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac
    39241 aactggcagc tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc
    39301 gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc
    39361 atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc
    39421 gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc
    39481 acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac
    39541 aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc
    39601 attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggcggcct
    39661 tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc
    39721 aggttatcgg atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc
    39781 gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg
    39841 cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca
    39901 accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca
    39961 cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca
    40021 atcgcataac cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga
    40081 acgataaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata
    40141 cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg
    40201 aacatgaagg agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg
    40261 gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca
    40321 attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca
    40381 gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg
    40441 gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggc
    40501 accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca
    40561 gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca
    40621 acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag
    40681 aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc
    40741 gcaccggaga taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct
    40801 tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca
    40861 taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca
    40921 ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca
    40981 tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct
    41041 gcgataggca gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca
    41101 aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc
    41161 acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat
    41221 tacacagagt aaaataattc agtgccgatt tgtttgacgg ctatcacgtt tcaacttcag
    41281 atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta
    41341 ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta
    41401 aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg
    41461 aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg
    41521 tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc
    41581 gcaaggtaag gtgagtcagt ttccaccagt aaccgatcca gggggacata acgcgcagca
    41641 tctcgaagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg
    41701 agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg
    41761 caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta
    41821 tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct
    41881 tgctgacgaa ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct
    41941 acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg
    42001 ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc
    42061 atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg
    42121 gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg
    42181 agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag
    42241 cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc
    42301 gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat
    42361 agcctgcagg cgcgagggag aaagatggtt tgccagttcg acgacaaggc ccggcacatc
    42421 aacattggtc acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc
    42481 cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca
    42541 gtcgcccgat ggcacgctat atgccaatgc ctgacacaat gtttcacgag cctgccagtt
    42601 atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc
    42661 aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc
    42721 cggcggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc
    42781 aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag
    42841 caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc
    42901 accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtactg catcaatgcc
    42961 cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg
    43021 catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag
    43081 taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat
    43141 gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg
    43201 tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg
    43261 cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta
    43321 tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct
    43381 tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta
    43441 acatcgagat agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt
    43501 gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg
    43561 tggcgatcgc caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc
    43621 agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg
    43681 cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca
    43741 ggttcccgag tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca
    43801 ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc
    43861 atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt
    43921 ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc
    43981 cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa
    44041 accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt
    44101 gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc
    44161 aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt
    44221 ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc
    44281 gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt
    44341 gagtaacgcg acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc
    44401 ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc
    44461 actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg
    44521 catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca gcaatttcag
    44581 catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag
    44641 atccggttcg atacgcagca accattgaaa aacccgtggg cgattgatga tcttatcggc
    44701 ataaagctgt tcaccaagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc
    44761 ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc
    44821 ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa
    44881 ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca
    44941 tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac catctcatct
    45001 gcctgcaacg cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc
    45061 agcaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagacgcggc
    45121 gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac
    45181 ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc
    45241 aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc
    45301 cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga
    45361 taaaccgtaa cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc
    45421 ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc
    45481 gttttcatct cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa
    45541 gcatcctgta gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg
    45601 gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc
    45661 tgcttatgac cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca
    45721 gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc
    45781 ttttatatgg gtgggaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc
    45841 caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat
    45901 gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa
    45961 cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta
    46021 acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga
    46081 agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg
    46141 cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg
    46201 ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca
    46261 taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact
    46321 cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc
    46381 acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa
    46441 aaccaccaac acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag
    46501 catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg
    46561 cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca
    46621 ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca
    46681 tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg
    46741 caccaatgcg ggttgcgttc tcttccgtta tatcaaggcc agaatcctgc atggcctgaa
    46801 cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga
    46861 taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag
    46921 tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag
    46981 actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct
    47041 tagacacgtt tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac
    47101 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    47161 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    47221 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt
    47281 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    47341 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    47401 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    47461 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    47521 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    47581 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    47641 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    47701 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    47761 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    47821 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    47881 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    47941 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa
    48001 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    48061 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    48121 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc
    48181 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    48241 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    48301 ttccttcaaa attcatgatt ttcctctttt aaagctcaag cgccgctgcc atcgctgaag
    48361 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    48421 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    48481 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    48541 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    48601 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    48661 acggtacgct cactggtaac ggcagagcac gttttgcgcc cgcagcttta caggcagcgc
    48721 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    48781 ttaccggaga aacgacctga cctgctgcag cttcttcaca cgctttcgca atagacgcat
    48841 catccagacc gatgattgcc gccatagcgc ccgtaccttc cggtacggct tcttgcatga
    48901 gcttgccgcg catctcaacc agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa
    48961 ccagcgcgga atattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    49021 gctgctgcca tacgcggtac agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc
    49081 aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca
    49141 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    49201 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    49261 tttaatcctt atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg
    49321 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    49381 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    49441 ccatagacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    49501 gaaccagcca gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga
    49561 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    49621 ttgaattctc tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac
    49681 cgtcggcatg cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag
    49741 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg
    49801 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    49861 cggctacgct taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc
    49921 aacctttaat gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    49981 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    50041 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    50101 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    50161 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    50221 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    50281 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    50341 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    50401 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    50461 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    50521 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    50581 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    50641 atcgtcttta atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    50701 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    50761 accgctgagg catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc
    50821 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    50881 gcaaattgca ccaacattgt actgtcacaa tcgacgttgg cccctaagtc aaggaccacc
    50941 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    51001 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    51061 caggcttgcg ctcgaccttc tttcaccagc tccagggcca cgcgcattga actcccacga
    51121 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    51181 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    51241 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    51301 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    51361 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    51421 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    51481 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    51541 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    51601 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    51661 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    51721 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    51781 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    51841 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    51901 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    51961 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    52021 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    52081 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    52141 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    52201 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    52261 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    52321 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    52381 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    52441 ggaaaaactg caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag
    52501 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc
    52561 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    52621 aatcaccggt aaaccgttaa cggaagaaaa tgcctgtctg caattacgca aagccagcgg
    52681 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    52741 aacagaagtg gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa
    52801 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    52861 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    52921 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    52981 tgcgctggat gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa
    53041 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    53101 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    53161 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    53221 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    53281 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    53341 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    53401 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    53461 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    53521 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    53581 ttttcgctat cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    53641 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    53701 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    53761 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    53821 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    53881 gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg taaatcatac
    53941 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt
    54001 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    54061 attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta
    54121 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    54181 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    54241 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    54301 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    54361 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    54421 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    54481 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    54541 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    54601 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    54661 cgcgttgccc ttgtagatgg gcagcgtctt tatgacctgg atatcgaaag tccagggcac
    54721 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    54781 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    54841 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    54901 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    54961 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    55021 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    55081 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    55141 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    55201 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    55261 gttcgcgcat tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg
    55321 aaagtgctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    55381 aaaatcaaac tgtataccgg cgagatcccg ctgttcagcc actaccagat tgagtcgcag
    55441 atcgagtccg ccttccagcg tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac
    55501 agcaccgaag cgttaacggc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    55561 atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg
    55621 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc
    55681 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    55741 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    55801 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    55861 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    55921 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    55981 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    56041 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    56101 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    56161 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    56221 ccggatgtgc cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    56281 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    56341 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    56401 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    56461 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    56521 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt
    56581 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg
    56641 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    56701 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    56761 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    56821 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    56881 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    56941 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    57001 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    57061 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    57121 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    57181 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactggggtc
    57241 cctgtcgccg ccgctgtcga acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa
    57301 gttgtagaag cccccgttca gattgccgaa ccgcaaccgg aagtggttga aacgacgcat
    57361 cctgaagtga ttgccgccgc agtaactgaa cagccgcagg tgattaccga gtctgatgtt
    57421 gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg
    57481 gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt
    57541 gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg
    57601 gttgaacctg aggtcaccgt tgagcataac cacgctacgg tgccgatgac gcgcgctccg
    57661 gcaccggaat atgttccgga tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc
    57721 ttcgaaggta aaggtgccgc aggtggtcat acggcaacac atcatgcctc tgccgctcct
    57781 gcgcgtccgc aacctgttga gtaataatta gctcaagtaa tcaagccctg gtaactgcca
    57841 gggctttttt atttcatctt tgaatcaaca tattaaaatt catcgaatgg catccttgct
    57901 aaccaacaat gcaaaatagg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac
    57961 aaaacatacc cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc
    58021 gacaatccct gcatatcggt aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg
    58081 atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt
    58141 tgccctaaag cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg
    58201 cctaattccg cgcgaacggt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt
    58261 ttatctaacg ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc
    58321 agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct
    58381 ggttccgcta ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc
    58441 accatcgaac gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc gacataatca
    58501 ccgttagcct cactaaacgg cgcagtctct gttttataac cggcaaaaat gtagcgccca
    58561 ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta
    58621 gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc
    58681 tgagcattct ggatagcagt ggtgacctgg ctaagtacac tctcttccag tgacactttt
    58741 tgagtggcga aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga
    58801 acaaccgctt gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc
    58861 gacatctgtt cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt
    58921 tgctggtaca tcatctgggt actgaaacgc attttatctc cccttagcga atgttaatca
    58981 gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct
    59041 gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct
    59101 gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg
    59161 ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc
    59221 ccaccgtttt actgttgctt tgcagatcca gcagggcctg accgttgcgg ttatcgctat
    59281 cacccgcatc tttttcgctc gccatcgcta ttttcgcttc gtcggtgatt aatacatcca
    59341 tgttgacgat ggcgtcactt actggtttca gcgtgaagct gtcgttaacg gcaggcgttc
    59401 ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg
    59461 taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg aacgagattt
    59521 tgtaatctgt cgccagtacc acggaggcat cagttaccgt ggcaccgatc gcaacgtcac
    59581 ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag
    59641 catcgccgtt agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
    59701 gcgccagttg tccaagcgta ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa
    59761 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc
    59821 catcaacata agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt
    59881 gccgcgccgt acttccctga accagtgagt aaccattggc caccgtgatg ttataagtgc
    59941 cgccatcctg aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat
    60001 cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga
    60061 tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca acgctggcac
    60121 ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact
    60181 gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt
    60241 tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg
    60301 tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc
    60361 tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca
    60421 caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct
    60481 gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac
    60541 ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc
    60601 tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag cgacccgccg
    60661 gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc
    60721 gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt gaggatcggt
    60781 agcatacccc gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac
    60841 ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg cttccagata
    60901 cgagctgtag acccgaaatt tcgcttttac tttcttcgct tcgccatttt catattcagt
    60961 cgtggtgatt tcagtaactg gacctttcca gttgccagag gctttgacgc caaacaggtt
    61021 atagctcggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag actccagcgc
    61081 cgcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca gttgggcggg
    61141 cagcgagagt tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg
    61201 tggcacggcc ttttgcacca gctgcgaaag cgcctgattt tgataacgca ccacagtttc
    61261 gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt
    61321 catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca tctgttgggc
    61381 aatctgctgg tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc
    61441 ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg
    61501 acgggccacc ggacggatat ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt
    61561 gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg
    61621 atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcagtat ggacatcaga
    61681 tccatcggcg tagcgcccag cgcattgagc gcgcgcacca cgttattgag gctggcgctg
    61741 gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta
    61801 accacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc ctgacgattg
    61861 actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc
    61921 atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta
    61981 acctgcatat tctggatatc tgcaaggaaa cggacctggg aactgttgcc acttggtacg
    62041 cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg
    62101 atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg caaattaagg
    62161 gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc
    62221 cgtccaccgt tcagttggtt cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg
    62281 ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc cttaagcggc
    62341 gtcatcaaca acgtacctcc acgcaagctt ttggcatttc ccatggaaga aaccaccaca
    62401 tcgatggttt gcccctgacg tccaaacgga ggaagtgact ctgtcaccat taccgcagcg
    62461 acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg
    62521 ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccggt gccatccagc
    62581 cccaccacca gaccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga
    62641 tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa tgcagagaga
    62701 aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa cgctgcaacc
    62761 agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaatg cgcgcatccg
    62821 ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag
    62881 agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc accacatgca
    62941 ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc
    63001 tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac
    63061 gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt
    63121 taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca
    63181 cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc
    63241 catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg
    63301 gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc caggcgcagc
    63361 cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca
    63421 tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttaa gccttagagt
    63481 tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa
    63541 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    63601 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca
    63661 ccagaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagccc ggtgtcattc
    63721 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt
    63781 acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc
    63841 gctggctgca cctgaaaacc accagctgcc cgttctgatc cacctggaaa gagccgtcac
    63901 gggtataggc tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg
    63961 cgacatcttt gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg
    64021 cgaccgggcg tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag
    64081 actgtgcccc cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac
    64141 gcttaaaacc gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt
    64201 gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag
    64261 tggattagtg acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg
    64321 cctacaagtg ctcgcaaaat cgataaattg cgattgcccc catagacccg ataaacttgc
    64381 gcatcgggca acttatactt gtcatgtatc ctttcaatta actcatcgac agcagttggt
    64441 tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc
    64501 gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa
    64561 cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg
    64621 tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt
    64681 tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg
    64741 ccgagattgt gccatcggca gcaatagtga tttccgctcc ttccggcaca gcaattggcc
    64801 ccgcctcgcc tatcacctga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa
    64861 tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccagccat
    64921 cctgctgcaa cgcgacgtcc agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat
    64981 ctgcgcccgg cgttgacgcc gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg
    65041 gcaccgcgcg taaagcattc aactgcgcgc gaaaaccggg cgttgaggca ttggccagat
    65101 tactggcggt taccgcctgt tgattcagtg tctggctggc tgctcccatc gcggtataaa
    65161 ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag cgtgttgagg
    65221 atctggtcct gagttttgat ggtctgggcg ttagactgat agttacgctg ggcaacgatc
    65281 atattgacca gttctttact gagatcgaca ttggacgctt ccaacgcacc gttggtcagg
    65341 gtgccaaagt tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca
    65401 gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca
    65461 atctgcccca gcagttgtgt ttgttcgttg gaatagttgc cgacaaccgt accgtcatca
    65521 ttgatttgat aactcaccag atcgcccggt ttgtagccat tctgggtggt tgccacaata
    65581 ttgttagcgc cggtattttg ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga
    65641 tctgcgccgt taattgcgcc agtggtaata ttctctgttg gatttgaggt cagaacgcca
    65701 ttggcattaa acaccagctt cattgcaggc tcggctgtac ctgttggatc actgctatcc
    65761 tgggtgtaga cctgccagtt attatccccg gtcttcacaa agtagacgct catgtcatga
    65821 gcattaccct gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg
    65881 ctggcgctga atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc
    65941 gacgccgtgg tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga
    66001 ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa
    66061 ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag
    66121 aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc
    66181 agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact
    66241 tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat
    66301 ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg
    66361 gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag
    66421 gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc
    66481 gtattaccgc tgttgccgcg gatcacaccc tgcaccagag caaactgcag cggctgggca
    66541 accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca
    66601 gtgctgccat cagtcaacgt accgtcccag gtgaaactgt ggactccggc ggtcagttca
    66661 ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg
    66721 tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca
    66781 ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc gatcaggtta
    66841 ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg
    66901 agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt
    66961 tccattggat tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt cagaaaactg
    67021 ctttgtaagt ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg
    67081 gtatttgtcg gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga
    67141 gcgtaagggt tttcagcatc atgcttttca ccatgttgag cacttcaaca ttggcctgat
    67201 agctgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt
    67261 ttacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt ttgtccgggg
    67321 cctgactttc tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    67381 taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg gtcacgctat
    67441 cagcattcgc cagattactg gccgccacgt tcaagcgctg ggactgggca gttaacgccg
    67501 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    67561 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    67621 tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct
    67681 ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt
    67741 gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga
    67801 cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat
    67861 tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt
    67921 gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct
    67981 gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg
    68041 ctatttatgc cgttgatgac cattgcggac aggtacaatt cacgttgtag aaatggctgg
    68101 gggcgaaaat gctggcaata aaacgtagcg tggcgatcat ctcgatacta ttcactccgt
    68161 taagtgcggc gagtaatctc acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg
    68221 gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccgtgcg
    68281 agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg
    68341 cacgctgcgg taacgacaaa cgatatttac aggttaatgt acaggccaca ggaaattatg
    68401 tggttgccgc gatgcccatt gtgcggggag gaaagctgga agctggcaat gtcaaactaa
    68461 aacgcggacg gcaggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg
    68521 atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc
    68581 aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt aacgggttta
    68641 gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc
    68701 gcatggtatc gggactggtg gtcagcggcg ttgttgatgc agatgggaat attcttataa
    68761 accagtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa
    68821 gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcccgtaa
    68881 gcaccgttca accacgcgaa accactgacg cgtcggtaac gaacacccgg gcggcaaaaa
    68941 caacagcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    69001 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    69061 gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag
    69121 acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct
    69181 gtgcttaacg atctcaaaac ggtaatggat caagaacagc aacatctctc tatggggcag
    69241 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    69301 gattacctcg aacagttacg caggaaagaa cccaatacag caaatagcgt tgatattagt
    69361 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    69421 ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    69481 catcaggaac cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt
    69541 aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta
    69601 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    69661 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc
    69721 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    69781 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    69841 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    69901 tgacagcccg gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    69961 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    70021 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    70081 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    70141 cggacccgaa agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg
    70201 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    70261 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat
    70321 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    70381 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    70441 tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    70501 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    70561 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    70621 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    70681 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    70741 tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac
    70801 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    70861 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    70921 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    70981 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    71041 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    71101 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga cccacgggca
    71161 aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg
    71221 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    71281 tacgttgcgc cagcactgcc tgttcgccgg cggtttgcag aactgtctga ttttgcacac
    71341 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac
    71401 caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg
    71461 taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    71521 ggcgatgcat acaggtggtg atctgcaaag gatcggccga aaaatggtgc tcggcaaaca
    71581 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    71641 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    71701 cgtgtggccc gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga
    71761 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    71821 tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca
    71881 gcggtttatc gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt
    71941 ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg
    72001 aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg
    72061 accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    72121 gcgcaatgcc gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga
    72181 tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg
    72241 acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc
    72301 tctacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    72361 agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccact
    72421 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    72481 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga
    72541 tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca
    72601 ctcaccgtgc gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat
    72661 tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca
    72721 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc
    72781 acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    72841 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    72901 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    72961 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    73021 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    73081 cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac
    73141 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    73201 atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc
    73261 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag
    73321 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    73381 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    73441 aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc
    73501 attttctgca ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa
    73561 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    73621 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    73681 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    73741 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    73801 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    73861 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    73921 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    73981 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    74041 acgttgtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc
    74101 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    74161 tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    74221 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    74281 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    74341 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    74401 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    74461 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    74521 catggtcagc ggtctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    74581 cattgccgag cctgcgcgtg aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg
    74641 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    74701 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    74761 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    74821 cgcaacgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    74881 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    74941 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    75001 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    75061 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    75121 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    75181 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    75241 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    75301 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    75361 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact
    75421 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    75481 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    75541 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    75601 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    75661 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    75721 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    75781 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    75841 tacccggcgt ggctgacgcc catattgttc tgacaaactt gatcagccaa attggtcgcg
    75901 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    75961 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    76021 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    76081 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    76141 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    76201 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    76261 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    76321 ttgtgtccgg caaaaacatt ccttcagccg gagcatagag attaatgact gcaccatccc
    76381 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    76441 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    76501 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg
    76561 ccgtacctgc cgggcacgat ttcaccccat tgataacctg ttatttaaca gattcgctgg
    76621 atcctaatga gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc
    76681 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    76741 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    76801 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    76861 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    76921 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    76981 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    77041 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    77101 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    77161 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    77221 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatgggt
    77281 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    77341 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    77401 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt atttaacctg tataaataac
    77461 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    77521 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    77581 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    77641 attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    77701 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    77761 ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa
    77821 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    77881 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    77941 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    78001 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    78061 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    78121 gaccccaaat aagtccgagc aggaagggac tgaagttggt caaacgctct ggttaaccac
    78181 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    78241 tgatttccag gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    78301 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    78361 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    78421 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    78481 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    78541 ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg
    78601 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    78661 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    78721 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    78781 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    78841 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    78901 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggttg acggtgagta
    78961 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagca
    79021 gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    79081 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    79141 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    79201 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    79261 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    79321 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    79381 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    79441 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    79501 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    79561 cggctgttgc actatctca
//
LOCUS       DAQSWQ010000004        75015 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.004, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000004 DAQSWQ010000000
VERSION     DAQSWQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.004"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..424
                     /locus_tag="R8G18_000258"
     CDS             <1..424
                     /locus_tag="R8G18_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HEE9794067.1"
                     /translation="DKENSLKRVINSFNSELMGEMSNNNIKVHLNEPEIIFLHAVLQQ
                     YLSQSCGAFVCMAAQEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLV
                     NRNCYLDKYGDANINDYYRDLEIKHSQRKNRASGKRVS"
     gene            813..1443
                     /locus_tag="R8G18_000259"
                     /pseudo
     CDS             813..1443
                     /locus_tag="R8G18_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1546..2048
                     /locus_tag="R8G18_000260"
                     /pseudo
     CDS             1546..2048
                     /locus_tag="R8G18_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(2115..3572)
                     /gene="nuoN"
                     /locus_tag="R8G18_000261"
     CDS             complement(2115..3572)
                     /gene="nuoN"
                     /locus_tag="R8G18_000261"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEE9794068.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3579..5108)
                     /gene="nuoM"
                     /locus_tag="R8G18_000262"
     CDS             complement(3579..5108)
                     /gene="nuoM"
                     /locus_tag="R8G18_000262"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEE9794069.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5272..7113)
                     /gene="nuoL"
                     /locus_tag="R8G18_000263"
     CDS             complement(5272..7113)
                     /gene="nuoL"
                     /locus_tag="R8G18_000263"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEE9794070.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7110..7412)
                     /gene="nuoK"
                     /locus_tag="R8G18_000264"
     CDS             complement(7110..7412)
                     /gene="nuoK"
                     /locus_tag="R8G18_000264"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEE9794071.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7409..7963)
                     /gene="nuoJ"
                     /locus_tag="R8G18_000265"
     CDS             complement(7409..7963)
                     /gene="nuoJ"
                     /locus_tag="R8G18_000265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEE9794072.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7975..8517)
                     /gene="nuoI"
                     /locus_tag="R8G18_000266"
     CDS             complement(7975..8517)
                     /gene="nuoI"
                     /locus_tag="R8G18_000266"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEE9794073.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8532..9509)
                     /gene="nuoH"
                     /locus_tag="R8G18_000267"
     CDS             complement(8532..9509)
                     /gene="nuoH"
                     /locus_tag="R8G18_000267"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEE9794074.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9506..12232)
                     /gene="nuoG"
                     /locus_tag="R8G18_000268"
     CDS             complement(9506..12232)
                     /gene="nuoG"
                     /locus_tag="R8G18_000268"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEE9794075.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12285..13622)
                     /gene="nuoF"
                     /locus_tag="R8G18_000269"
     CDS             complement(12285..13622)
                     /gene="nuoF"
                     /locus_tag="R8G18_000269"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEE9794076.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13619..14119)
                     /gene="nuoE"
                     /locus_tag="R8G18_000270"
     CDS             complement(13619..14119)
                     /gene="nuoE"
                     /locus_tag="R8G18_000270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEE9794077.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14122..15924)
                     /gene="nuoC"
                     /locus_tag="R8G18_000271"
     CDS             complement(14122..15924)
                     /gene="nuoC"
                     /locus_tag="R8G18_000271"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HEE9794078.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16018..16680)
                     /gene="nuoB"
                     /locus_tag="R8G18_000272"
     CDS             complement(16018..16680)
                     /gene="nuoB"
                     /locus_tag="R8G18_000272"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEE9794079.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16696..17139)
                     /gene="nuoA"
                     /locus_tag="R8G18_000273"
     CDS             complement(16696..17139)
                     /gene="nuoA"
                     /locus_tag="R8G18_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HEE9794080.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17768..18706)
                     /gene="lrhA"
                     /locus_tag="R8G18_000274"
     CDS             complement(17768..18706)
                     /gene="lrhA"
                     /locus_tag="R8G18_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HEE9794081.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19626..20843
                     /gene="alaA"
                     /locus_tag="R8G18_000275"
     CDS             19626..20843
                     /gene="alaA"
                     /locus_tag="R8G18_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HEE9794082.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20927..21526
                     /gene="yfbR"
                     /locus_tag="R8G18_000276"
     CDS             20927..21526
                     /gene="yfbR"
                     /locus_tag="R8G18_000276"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HEE9794083.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21585..23417)
                     /locus_tag="R8G18_000277"
     CDS             complement(21585..23417)
                     /locus_tag="R8G18_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEE9794084.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23504..24152)
                     /gene="hxpA"
                     /locus_tag="R8G18_000278"
                     /pseudo
     CDS             complement(23504..24152)
                     /gene="hxpA"
                     /locus_tag="R8G18_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(24163..24657)
                     /locus_tag="R8G18_000279"
     CDS             complement(24163..24657)
                     /locus_tag="R8G18_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEE9794085.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24740..25195)
                     /locus_tag="R8G18_000280"
     CDS             complement(24740..25195)
                     /locus_tag="R8G18_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HEE9794086.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25533..26735
                     /gene="ackA"
                     /locus_tag="R8G18_000281"
     CDS             25533..26735
                     /gene="ackA"
                     /locus_tag="R8G18_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEE9794087.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26810..28954
                     /gene="pta"
                     /locus_tag="R8G18_000282"
     CDS             26810..28954
                     /gene="pta"
                     /locus_tag="R8G18_000282"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEE9794088.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29144..30664
                     /gene="yfcC"
                     /locus_tag="R8G18_000283"
     CDS             29144..30664
                     /gene="yfcC"
                     /locus_tag="R8G18_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEE9794089.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30697..31239)
                     /gene="yfcD"
                     /locus_tag="R8G18_000284"
     CDS             complement(30697..31239)
                     /gene="yfcD"
                     /locus_tag="R8G18_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HEE9794090.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31297..31848)
                     /gene="yfcE"
                     /locus_tag="R8G18_000285"
     CDS             complement(31297..31848)
                     /gene="yfcE"
                     /locus_tag="R8G18_000285"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEE9794091.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31904..32548)
                     /gene="yfcF"
                     /locus_tag="R8G18_000286"
     CDS             complement(31904..32548)
                     /gene="yfcF"
                     /locus_tag="R8G18_000286"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HEE9794092.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32684..33331
                     /gene="yfcG"
                     /locus_tag="R8G18_000287"
     CDS             32684..33331
                     /gene="yfcG"
                     /locus_tag="R8G18_000287"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HEE9794093.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33388..33750
                     /gene="folX"
                     /locus_tag="R8G18_000288"
     CDS             33388..33750
                     /gene="folX"
                     /locus_tag="R8G18_000288"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HEE9794094.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33771..34664
                     /locus_tag="R8G18_000289"
     CDS             33771..34664
                     /locus_tag="R8G18_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEE9794095.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34712..35602)
                     /gene="rpnB"
                     /locus_tag="R8G18_000290"
     CDS             complement(34712..35602)
                     /gene="rpnB"
                     /locus_tag="R8G18_000290"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HEE9794096.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35799..36572)
                     /gene="hisP"
                     /locus_tag="R8G18_000291"
     CDS             complement(35799..36572)
                     /gene="hisP"
                     /locus_tag="R8G18_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HEE9794097.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36580..37296)
                     /locus_tag="R8G18_000292"
     CDS             complement(36580..37296)
                     /locus_tag="R8G18_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9794098.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37293..37979)
                     /gene="hisQ"
                     /locus_tag="R8G18_000293"
     CDS             complement(37293..37979)
                     /gene="hisQ"
                     /locus_tag="R8G18_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HEE9794099.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38069..38851)
                     /gene="hisJ"
                     /locus_tag="R8G18_000294"
     CDS             complement(38069..38851)
                     /gene="hisJ"
                     /locus_tag="R8G18_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HEE9794100.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39072..39854)
                     /gene="argT"
                     /locus_tag="R8G18_000295"
     CDS             complement(39072..39854)
                     /gene="argT"
                     /locus_tag="R8G18_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HEE9794101.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40120..40689)
                     /gene="ubiX"
                     /locus_tag="R8G18_000296"
     CDS             complement(40120..40689)
                     /gene="ubiX"
                     /locus_tag="R8G18_000296"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEE9794102.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40784..42301)
                     /gene="purF"
                     /locus_tag="R8G18_000297"
     CDS             complement(40784..42301)
                     /gene="purF"
                     /locus_tag="R8G18_000297"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEE9794103.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42338..42826)
                     /gene="cvpA"
                     /locus_tag="R8G18_000298"
     CDS             complement(42338..42826)
                     /gene="cvpA"
                     /locus_tag="R8G18_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HEE9794104.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43085..43747)
                     /gene="dedD"
                     /locus_tag="R8G18_000299"
     CDS             complement(43085..43747)
                     /gene="dedD"
                     /locus_tag="R8G18_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HEE9794105.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43737..45005)
                     /gene="folC"
                     /locus_tag="R8G18_000300"
     CDS             complement(43737..45005)
                     /gene="folC"
                     /locus_tag="R8G18_000300"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEE9794106.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45075..45989)
                     /gene="accD"
                     /locus_tag="R8G18_000301"
     CDS             complement(45075..45989)
                     /gene="accD"
                     /locus_tag="R8G18_000301"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEE9794107.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46145..46804)
                     /locus_tag="R8G18_000302"
     CDS             complement(46145..46804)
                     /locus_tag="R8G18_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEE9794108.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46887..47699)
                     /gene="truA"
                     /locus_tag="R8G18_000303"
     CDS             complement(46887..47699)
                     /gene="truA"
                     /locus_tag="R8G18_000303"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEE9794109.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47699..48712)
                     /locus_tag="R8G18_000304"
     CDS             complement(47699..48712)
                     /locus_tag="R8G18_000304"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEE9794110.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48778..49914)
                     /gene="pdxB"
                     /locus_tag="R8G18_000305"
     CDS             complement(48778..49914)
                     /gene="pdxB"
                     /locus_tag="R8G18_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HEE9794111.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50013..51008
                     /gene="flk"
                     /locus_tag="R8G18_000306"
     CDS             50013..51008
                     /gene="flk"
                     /locus_tag="R8G18_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HEE9794112.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(51005..52183)
                     /locus_tag="R8G18_000307"
     CDS             complement(51005..52183)
                     /locus_tag="R8G18_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9794113.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52458..53678)
                     /gene="fabB"
                     /locus_tag="R8G18_000308"
     CDS             complement(52458..53678)
                     /gene="fabB"
                     /locus_tag="R8G18_000308"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEE9794114.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53837..55843
                     /gene="mnmC"
                     /locus_tag="R8G18_000309"
     CDS             53837..55843
                     /gene="mnmC"
                     /locus_tag="R8G18_000309"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEE9794115.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55964..56242)
                     /locus_tag="R8G18_000310"
     CDS             complement(55964..56242)
                     /locus_tag="R8G18_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HEE9794116.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(56276..56824)
                     /gene="epmC"
                     /locus_tag="R8G18_000311"
     CDS             complement(56276..56824)
                     /gene="epmC"
                     /locus_tag="R8G18_000311"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HEE9794117.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56824..57633)
                     /locus_tag="R8G18_000312"
     CDS             complement(56824..57633)
                     /locus_tag="R8G18_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEE9794118.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57633..58457)
                     /gene="mepA"
                     /locus_tag="R8G18_000313"
     CDS             complement(57633..58457)
                     /gene="mepA"
                     /locus_tag="R8G18_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEE9794119.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58461..59546)
                     /gene="aroC"
                     /locus_tag="R8G18_000314"
     CDS             complement(58461..59546)
                     /gene="aroC"
                     /locus_tag="R8G18_000314"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEE9794120.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59581..60513)
                     /gene="prmB"
                     /locus_tag="R8G18_000315"
     CDS             complement(59581..60513)
                     /gene="prmB"
                     /locus_tag="R8G18_000315"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEE9794121.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            60679..61230
                     /gene="smrB"
                     /locus_tag="R8G18_000316"
     CDS             60679..61230
                     /gene="smrB"
                     /locus_tag="R8G18_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HEE9794122.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61352..62224)
                     /locus_tag="R8G18_000317"
     CDS             complement(61352..62224)
                     /locus_tag="R8G18_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HEE9794123.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(62211..62735)
                     /locus_tag="R8G18_000318"
     CDS             complement(62211..62735)
                     /locus_tag="R8G18_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9794124.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62732..63202)
                     /locus_tag="R8G18_000319"
     CDS             complement(62732..63202)
                     /locus_tag="R8G18_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9794125.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(63199..63747)
                     /locus_tag="R8G18_000320"
     CDS             complement(63199..63747)
                     /locus_tag="R8G18_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9794126.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63722..64473)
                     /locus_tag="R8G18_000321"
                     /pseudo
     CDS             complement(63722..64473)
                     /locus_tag="R8G18_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64493..67135)
                     /gene="yfcU"
                     /locus_tag="R8G18_000322"
                     /pseudo
     CDS             complement(64493..67135)
                     /gene="yfcU"
                     /locus_tag="R8G18_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(67217..67780)
                     /locus_tag="R8G18_000323"
     CDS             complement(67217..67780)
                     /locus_tag="R8G18_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEE9794127.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68464..68949)
                     /gene="sixA"
                     /locus_tag="R8G18_000324"
     CDS             complement(68464..68949)
                     /gene="sixA"
                     /locus_tag="R8G18_000324"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEE9794128.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(69152..71296)
                     /gene="fadJ"
                     /locus_tag="R8G18_000325"
     CDS             complement(69152..71296)
                     /gene="fadJ"
                     /locus_tag="R8G18_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HEE9794129.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(71296..72606)
                     /gene="fadI"
                     /locus_tag="R8G18_000326"
     CDS             complement(71296..72606)
                     /gene="fadI"
                     /locus_tag="R8G18_000326"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HEE9794130.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72786..73070)
                     /locus_tag="R8G18_000327"
     CDS             complement(72786..73070)
                     /locus_tag="R8G18_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HEE9794131.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73442..74782
                     /gene="fadL"
                     /locus_tag="R8G18_000328"
     CDS             73442..74782
                     /gene="fadL"
                     /locus_tag="R8G18_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HEE9794132.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT        18809 a        20198 c        18517 g        17491 t
ORIGIN      
        1 tgataaagaa aattcactta agcgtgtcat taatagtttt aattctgaac tcatgggaga
       61 aatgtcgaat aataatataa aagttcattt aaatgaacca gagataatat ttttacatgc
      121 cgttcttcag caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg cagcccagga
      181 agtgattgaa caaagggaaa gcaattctga cagcgccccc tatacgttat taaaaaacta
      241 tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt ttcaacatcg
      301 actggtaaac aggaattgtt atttagataa atatggcgat gcaaatatca atgattatta
      361 tagagactta gaaataaaac actcacaacg caaaaataga gcatcaggaa aaagagtaag
      421 ttaacatcat tgttaattaa ttcgaccagt cagcaaatat gctgactggt cgaacatagt
      481 ttgctaaaca gtcatcactg actgatgtca gtcacaccat ccgccagttc aatcttcggg
      541 tatttccagc gtatctcggc acgcgattgc tggtctgttg atgtagtgga ttgttgttgc
      601 tgacttttct taccctcaga gttctgcccc ctgtcaactc aatctgccaa taaataacat
      661 aattatattt ttccttatat gaattccttt ttaaaatagc gaagccactg taaaagatac
      721 tttttgtata gatctaacaa ccgtaataaa acaaacactc ataactcatt ataaataatg
      781 ataaaataac gtcttttatt gataatttat atatggaaaa aaattccttt tgttatattg
      841 tttctgattt cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa
      901 cgccccgaca cgtcagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg
      961 tgcatccacg taatgccgac aatattgata atctgaatag gtccgtagag tatataaaag
     1021 agttctttgt cagtagcggt gccagagtta ccgcgcagag cgtccccgat tggctattac
     1081 gactctgcgc ctggtagcca ggattaccct tacccgcaat gtcctggctt tatcccgatc
     1141 ggggggactt tattgcctgg tcggcagaat acaggatatc aacgccgttc gtcaggtaaa
     1201 aacggcattg ttatcatccc gggatttatc tgtttattct atgaatgccc catggtttat
     1261 tcccggtatt gatttctctg accacctgaa ttattggcaa cacgatattc ccgccgtaat
     1321 gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg atacagcaga
     1381 cagattgaat tatcagaaaa tggctcagat ggtgttataa ccttgttaca caacagtaaa
     1441 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1501 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggt
     1561 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1621 ttgatggata atatacatga cacgctttca ggattacgcc gactggatat tgataaacgc
     1681 tgggattttt tacactttgg gctgacaggc acccctgctt ttgatcccgc gaagaatgat
     1741 ccgctgagtc gtgccgtgct tggtgaacac agcctcgaag atggcataga tggcttcctg
     1801 ggattgacct ggaatcagga gttagccgcg acaatcgacc gactggaatc actggatcga
     1861 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1921 gtgacctttt cagaagagtt agaggggcaa ctctttgctt cgatcatgct gatatggaaa
     1981 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     2041 ttgggtgatg gtgtcagggc accaaaaaaa accgccgaac atgtcggcgg ctttctgact
     2101 tacaaactga tagcttacat cagcggcatt gctaaccgca caatgctaat cagcggttgt
     2161 ggccatacac ccagcaccag taccaacagt gcagagatca gcaccacgat accgcctgcg
     2221 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggggcgtg caggtacagg
     2281 ctcaccgcca cgcgcaggta gtagtaaagg ccgcttgccg aaccgacaac cacggcgccc
     2341 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accaataaag
     2401 cccagcgtca tcggaatacc ggccagagac agcatcatca ccgtcatcac cgccgcgagg
     2461 atcgggcgat gccagaacag tccgcggtaa gagtacaggg aatcagcatc cgggccacga
     2521 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctaaa cagataaccg
     2581 gccaggtaaa cccctaccgc ttccatcgac atctcgccag tttgcagcgc aatcagcgcc
     2641 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatattggtc
     2701 tggctcagcg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagcacc
     2761 acgcgaatcg cttcgctgtc acccatcggt gcgtagagga acagacgcat caccacaccg
     2821 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2881 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2941 atcatcaggc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccaaggttt
     3001 ttacccaacg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccaaac
     3061 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagtgaa
     3121 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcgata
     3181 ccgaggaaca gagacgccag atggttggca ttcgccagca ggatcccgcc cagcgcggca
     3241 attaacacca acaggtagaa ctcgtccttg ttgtcgttat agccttcaag ccacgggtag
     3301 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3361 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcctg cctggccgac aaaccagagc
     3421 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3481 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3541 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3601 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3661 tgcggataga agccctgcag taccagcagc accaccagca acaggatcat aaacagctca
     3721 cgcagcgaca tccctggcag ttcctggctg gcaatctcgc ttttcgcttt accgaagtaa
     3781 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3841 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3901 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3961 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     4021 taaagctgac cacaaagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     4081 attaccgcgc cctggtaggc caactggctg ccggtgtaga tagcaatcag cacgaagccc
     4141 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgggc gaaggccatc
     4201 cacgcaccgt agaagatgcc gataacaccc agccacatag caattggcgc gaactctgcc
     4261 gacgcgttcg ggaacagcgg cagggagaaa cgcagcaaac cgtaagcggc agttttcagc
     4321 aagatccccg cgaggtcaac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4381 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatt
     4441 aacaggtatt ccgcaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4501 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcaat caacatcacc
     4561 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4621 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4681 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4741 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4801 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagcccgtca
     4861 atggcgagat gaatagagat acccaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4921 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4981 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     5041 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     5101 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     5161 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcaccg cgaaggcggc
     5221 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     5281 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     5341 cagatagccg ttctcactta acagcagacc tttacctgca aagcgggaaa ggacagccgg
     5401 aatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5461 cctgacgaac actttgtcgt acagccagtc aaagccccag gcgttgtacc accaggtact
     5521 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt tacccagcca
     5581 cagccaagct gccagcagaa tgccgacaat cgcgaccacg ccagaggtaa tttccagggt
     5641 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5701 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5761 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5821 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5881 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5941 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     6001 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     6061 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     6121 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     6181 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     6241 cagcgtaacc gccccgacaa tgcccaccag atgcagaatt tccggcgtca tcaggaacag
     6301 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atggttgcgg cgtggatcag
     6361 cgcggagaca ggcgtcgggc ccgccatcgc gtcggcaagc catgtctgca acggcaactg
     6421 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6481 gccgtcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca gggtgcccag
     6541 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6601 gaacgctttc attgccgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6661 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tgtcggcaag
     6721 caccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6781 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6841 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6901 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6961 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     7021 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     7081 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     7141 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     7201 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     7261 cttccggcga caacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     7321 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaatgaa taagattgcc
     7381 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7441 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7501 ccacaaccag acctgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7561 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7621 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccggac
     7681 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7741 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7801 ccagcgcacc ggcgaagtaa gcgcccagtg agaagaacac cccggaaatc gccagcagcg
     7861 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7921 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7981 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     8041 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     8101 gatcagcaga tcctcttttt cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     8161 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     8221 gcagcgtgag aagttgatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     8281 tttttgcagc gagatacagc cgaccgggca ggctaccgcg cagaggttac aggctacgca
     8341 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8401 atagatcggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8461 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8521 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8581 agcgtcagcg gcaggcagat tttccagccg aaggacatta cctggtcata acgcggacgc
     8641 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8701 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8761 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8821 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8881 tgacggtgac ataccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8941 ataacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     9001 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     9061 gaagcacgca tcgcacccag caacgagtat ttgttgttac ttgaccagcc cgcaaacagc
     9121 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     9181 gcaaccaccc aacccggact gactggcaca atcgcaaagg ccagcagcag cgaggtaaag
     9241 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     9301 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9361 ttaggtccgt aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9421 gccccgcagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9481 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9541 cgccagccag caccggagca ataccggaca tacccatcgg caagcccacc tgccctgccg
     9601 tcagcccttc agcgatttca accggcagcg tgaccgtatt gccatcgtaa ctaaaggaga
     9661 cgtgtgtacc tgcgttcaca cctaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9721 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9781 ggtggtaata cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9841 tggtgaagta atccagacca ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9901 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9961 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcagt cggctggttg ttaccttcca
    10021 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
    10081 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
    10141 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc tgccagttcc gggattttcg
    10201 ccacaacagc gtcaatcaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaggg
    10261 tgctgtgcag cgagtgtaac cagcgccagc tttccagcat gacagttttg ctgtcgtaat
    10321 aagcaggatc gtaaacctgg aagaaacgtt gggcgcggcc ttcgttgttg atcaccgtac
    10381 cgtcgctttc agcaaaactg gcagcagaaa gtaccagatg ggcgttttcc ataatcgctg
    10441 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10501 tagcggaagc gtgacgatgc agatcgtttt ccagcaccac caccgcgtcg gcgcgtccgg
    10561 tttccagttc ggtcaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10621 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10681 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10741 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ctttgcagct
    10801 caggttcgat accgtcaacc gctggcgcag agttatccag cgcatgggcg atggcaaaac
    10861 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10921 gggtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10981 ttgccgcaat ctgccagtca gccactttct gtgctgccgc catttcgcgc gctttacctt
    11041 tcacagcctg acgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    11101 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    11161 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    11221 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    11281 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    11341 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11401 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11461 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11521 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11581 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11641 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11701 tgtcgtgcgc accgtaaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11761 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11821 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacag
    11881 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11941 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    12001 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    12061 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    12121 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    12181 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    12241 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    12301 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12361 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12421 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12481 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12541 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12601 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12661 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12721 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12781 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12841 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12901 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12961 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    13021 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    13081 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    13141 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    13201 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    13261 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    13321 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13381 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc tccgccgcgc
    13441 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13501 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13561 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13621 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13681 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13741 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13801 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13861 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13921 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13981 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    14041 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    14101 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    14161 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    14221 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    14281 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    14341 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14401 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14461 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14521 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14581 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14641 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14701 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14761 cggcatccag tcgaggaact cacgcagcag gcgatcccag ccgcgcggca ggtcgtgcgc
    14821 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14881 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14941 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    15001 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    15061 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    15121 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    15181 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    15241 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt tttcggtgcc
    15301 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15361 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat ctttacgcag
    15421 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15481 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15541 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15601 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15661 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15721 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15781 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15841 ttcgccaatc accggatcat caagatgatc acgggtctgc caggcgggtt cttgcgcggt
    15901 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15961 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    16021 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    16081 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    16141 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    16201 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    16261 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    16321 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16381 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16441 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16501 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16561 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16621 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16681 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16741 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16801 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16861 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16921 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16981 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    17041 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    17101 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    17161 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    17221 aaaaacctta caatcactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    17281 cctttctata accttttgtc aactttaaca aaagtttctt cacattagtt tacataatat
    17341 caacaccatt agcatttaat gccctttcac gccagatcct tgacgactcc aggataatta
    17401 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17461 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17521 gtcattccat aacaaaaacg cagcaacaaa tttacgtatt tttaacatca ttgtagcagg
    17581 tgattttttt caggcgatta tttgtgcgtt cgggacgtga atctctggtg attgaaaaat
    17641 gaacagtttt gtacgttctg cactatgcga ggaaggattt ttactaaaaa aaagccgctg
    17701 gggtttaaaa cacccccagc ggctcgtttt tacactattg tctcaggaat tatctatcgt
    17761 ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc atccccttcc ggagcagaca
    17821 ttgggctgta ttgccacgga ttatggtagc tctccatcgc ctgatagatc acctgggcca
    17881 gctcattatt actggacggg tcatagcaca gcaaatattc ggtatcaggt agcggcggta
    17941 agccatctac tccactcagc acacgcaggt ccgggctcat catttcaatc ggccttgccg
    18001 tcacgccaag accggctttc actgccgcac gaacggccgg aagcgtcgag gcgacataag
    18061 ccagtcgcca tggaatatct gctttattaa gcgtcgccag caccatatcg cgaaacggac
    18121 ttggatcatc cagcaataca agagggatcg gctcgccttt ttgcaatacg tattccgctg
    18181 cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct gaaggacgat
    18241 gggtggttac catcaaatcc acttcctggg attccagcat ttctgccata taggcattac
    18301 gtttaacgcg gacatccagc gctaatttcg gataaaccga actcacgcga tttaacagga
    18361 aaggtaagat cgtatcggca gattcatctg aagcaccgat agttaacacg ccctgaagat
    18421 tactgaacat taatgatgag caggcctcat cattaaaacg cagtattttc ctggcgtaac
    18481 caagaagttg aatgccatgt tcagttaaca gtttgttgcg accgtgacga gcgaacagtt
    18541 ctttcccaac gagttgttcc agacgctgca tttgctgact tacggcggac tgagtacgac
    18601 acacagcggc agctgcggca gcaaaagtgt tcagatcggc aacagcaaca aatgttctca
    18661 gcagatcgag gtcgaggtta attatcggac gatttgcact tatcatatat tatcacttac
    18721 tggcggctca tactgagctg gttaatgctg tgcacacaca aacaagcaat tccatttgta
    18781 atgtgcctcc ctggcagttt catccggaac ccggacgaaa gtaaaaatgc atatgagttg
    18841 cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg aagtagatca cagaatatat
    18901 cttcagggat cgcatatcta ttaagttact cactcttttc tatttatgac atgcgcgtgt
    18961 ttgtataaat gtaaatgtga gtccttgttc cactctcgtg cagcatcgct ggtcatacgc
    19021 gaacacgtac aaacagcaat ggtgaggcgg catcaagagc agggatccgt catttatgcg
    19081 agcattttac cccaaaaacc ttttatttat aagggtcatt gcgaattatc tgatgcaaag
    19141 ttatgttatg ttaggcaaag taatcttcta ttattaataa gcacatcaaa acctttttga
    19201 atattaaata ataattaatt agcatcatcc tcattcatta attccgctta acaatagttt
    19261 cacaccttgc ccccattgcg acacctcgga aatcatcacg ctgttaaact ctccgtaatg
    19321 cggaaatatc atcatcgcgt ttcattagtg aattcttaaa tgaggcattt tcacacaatt
    19381 atcttacaga taaaaaaacc agacttgcaa ttaagaatca gaacaattca ctatataaca
    19441 ttgcatgcaa agcatataca cctcattatt ttgtcattat taagttatta acagcacaat
    19501 cgagccttcc cctctggcaa aatcttattc tgcagacctt caaaacaccg tcctggggga
    19561 gtacattgtt ctaagctgac ttccacggca gggagtggcg ataacagcaa aaaaggtcaa
    19621 gattcatgtc ccccattgaa aaatccagca aattagagaa tgtctgttat gacatccgtg
    19681 gtccggtgct gaaagaagca aaacgcctgg aagaagaagg taacaaggta ctgaaactga
    19741 acatcggcaa cccagcccca ttcggttttg acgcgccaga tgaaatcctc gttgacgtga
    19801 tacgcaacct gcctaccgct caagggtatt gcgattccaa agggctttac tccgcgcgta
    19861 aagccatcat gcagcactac caggctcgtg gcatgcgtga tgttaccgtg gaagatattt
    19921 acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat gcaggcattg ctgaacagcg
    19981 gggacgaaat gttggttcct gcaccagatt acccactctg gaccgcggcg gtttcgcttt
    20041 ccagcggtaa agcggtgcat tatctttgcg atgaatcctc tgactggttc ccggacctcg
    20101 atgatattcg cgctaaaatt acgcctcgta cgcgtgggat cgttattatc aacccaaata
    20161 acccaaccgg cgcggtatat tccaaagagc ttttaatgga gattgtggag attgcacgtc
    20221 agcataatct cattatcttc gccgatgaaa tttatgacaa aattctctac gacgatgctg
    20281 agcatcactc aattgcgcca ctggcacctg acctgctgac cattaccttt aacggactgt
    20341 cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat ggtgttgaac gggccgaaaa
    20401 aacacgccaa aggctacatc gaaggtctgg aaatgctggc ttcaatgcgc ctgtgtgcta
    20461 acgttcctgc gcaacacgcc attcagaccg cgctgggtgg ttatcagagc atcagtgaat
    20521 ttattacccc tggcggtcgt ctttatgagc agcgtaaccg cgcgtgggaa ctgatcaacg
    20581 atattccggg cgtttcctgc gtgaaacctc gtggtgcgct gtatatgttc ccgaaaatcg
    20641 acgccaaacg ctttaacatt cacgacgatc agaaaatggt gctggatttc ctgttgcagg
    20701 aaaaagttct gttggtgcaa gggacggcat tcaactggcc gtggccggat cacttccgca
    20761 ttgtcacgct accgcgtgtc gatgatatcg agctgtcttt gagcaagttc gcgcgtttcc
    20821 tttctggtta tcatcagctg taatcttaat ttcactgccg gagattgcat ccggcagcgt
    20881 tatcccgcca caatgacctg atgatgtcat catacgtaag gtcactatga aacagagcca
    20941 tttttttgcc catctctccc gcctgaaact cattaaccgc tggccgctaa tgcgcaacgt
    21001 gcggacggaa aatgtgtccg aacacagttt gcaggtagcg atggtcgccc atgcgctggc
    21061 agctatcaaa aatcgaaaat ttggcggtaa tgtcaacgcc gaacgtatcg ctttactggc
    21121 gatgtaccac gatgcctcag aagtgctcac cggcgatctc cctactccgg tgaaatactt
    21181 caattcgcaa atcgctcagg agtacaaggc tattgaaaaa atcgctcagc aaaaactggt
    21241 cgatatggtt ccggaagagc tgcgggatat ctttgcgccg ttaattgacg agcatgcata
    21301 tagcgatgaa gaaaaatcgc tggtgaaaca ggcagatgca ctgtgtgcat atctgaaatg
    21361 tctggaagaa ctcgcggccg gaaataatga attcttgctg gcaaaaacgc gactggaagc
    21421 gacgcttgaa gcgcgtcgca gccaggagat ggactacttc atggaagtat ttgttcccag
    21481 cttccatctt tcgctcgatg agattagcca ggattcaccg ctgtaagcag ccggagtctg
    21541 cgtcgcatca ggcaataagc gccggatgcg acatcaggct cttgtcaaaa cggaaacagc
    21601 atcgggatca tcaccacaca aaccgccatc acgataatgg tgaacggtac ccccaacttc
    21661 acaaagtcac tgaagctgta atttcccgga cctaaaacca gtgtgttaac aggtgaagaa
    21721 accggtgtca taaaggcggc ggatgctgcc atcgccacga ccatcgcgaa tggataaggc
    21781 gacaccccca tcgttttggc agcagccagc gcaatcggag ccatcaacac cgccgtcgcg
    21841 gtattagaga taaatagccc aataaccgcc gacaagacaa acaaacaccc cagcatcata
    21901 tgtggcccgt aaccaccgcc aatgtccatc agccctttca ccgccagcgc gacacctccc
    21961 gttttctgta atgccacagc aaacggcatc atcccaacga tcaaaataat gctcggccag
    22021 tgaatggatt tataggcgct ttcagcatct atacagcgga atttccccat cagcaggcag
    22081 gcgatgatag cggcaacagg attaggaatt tcatctgtca gcattaacgc caccattagc
    22141 accagacaga aaatggcatg gggtgcctgg ctgtgcgcgg gcgatgcttc actcacctct
    22201 tccggtaagt tcagcgctac gaagtcgcgg ccctgtttgg ccagcatacc gatcagtttc
    22261 cagttaccca caaccaggat gatatcgccc agcagcagag gctcatccgc cagcgaacct
    22321 tccagcgcca cgccattgcg ctttagcccc accacattca gtccgtagcg ggtacgaaaa
    22381 ccaatttcgc gcaccgattt accaatcagt tctgactcag gaattaatga aatctctgcc
    22441 atgcccacat caagggcctg gtcagaaaaa tactcgccgc gcagtaccat cggctccagc
    22501 aattgctcac tacaaaattg ccggagatcg acatcagccg cagacatatc aataagcaaa
    22561 acgtcacgcg cgcgaaattc agaaacccca ttaacgttca cgataacgcg acgaaaacgc
    22621 cgccagcgtt caacaccgat gacgttagcg ccataacgct cacgtaattt gagatcatcc
    22681 agccgttgac caatcattgg cgatccgggg cgaatagcca gacgtcgcgc acgcccggtc
    22741 agtcgatatt cacggataag atcgcgaaag gttcgacgcg tccagccttc gcgctgcggg
    22801 gtctgggtat cccctttcag catgaaacgc atcactaaca tatacaaaat acccagcacc
    22861 agcacgacca ggccaatagg tgttacgcta aagaaactga agccgtgata gccttcacgc
    22921 agcaattcac tgttgactac caggttcggc ggcgtcgcca ccagcgtcat catgccgctt
    22981 atcagcccgg caaaactcaa cggcatcatc agacgtgacg gcgaagtttg catacgcatg
    23041 gcaacgctta acaccacggg aataaagata gcgacaacgc cggttgaact cataaacgcg
    23101 ccaagccccg cgacggtcag catcaacaaa accaacattt tgatttcact gttgcccgca
    23161 actttgacca gccatgttcc cattacggtg gcaacaccgg tacggaccaa accatcgcca
    23221 ataataaaca aggcggcaat caggacaacg ttaggatcag aaaagccgga aaatacttcg
    23281 gggactgtca gcgttccgct taatgcaaac gcgacaataa caaacaaagc gaccgcatcc
    23341 atacgcactc tgcccgtcgc aaacaagaca atggcaacgg ccagtaatga aagaacccaa
    23401 atcaattcac cgttcacaac ttatccttgt taattgaggg agatgtcttg attctgccat
    23461 aaaaaagccc cgacgagacg gggctaaatc atgatcaggt gtttcactga ataataacat
    23521 cgccatttgg ctgtttggtc acagtaattt gctccagact gtggaggacc aaatcgacct
    23581 cattcaggcg cggggtatct gccggagcgt taaccgcaat gacatgacaa cccgccgcca
    23641 ggccagaaag cacgccagcg gagcatcttc caccaccaca cactcctgcg gcgcaagccc
    23701 cagcagctgc gcgcctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc
    23761 agcggttaca aacacctctg gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac
    23821 cggcatggag ccagaagtca caatggccca cggaatacct gctttattca aatgactgag
    23881 taaggcgatt gcccccggaa gcgggtaata ccttcggttt ccgtggcctc gatgtgctcc
    23941 agacgcgtaa actcggcggc aatatcagcc tcggatttgc ccgccataaa atggcgcaga
    24001 gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct cttccggcgc taacccatga
    24061 cgtctggccc agttgctcca cgcccgttct accgcaggca gggaatccac cagcgttcca
    24121 tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct cctcaggcat tgataatttg
    24181 attaatttcg ttggcgctca aatggtactg acgcgggcag gcatgccaca cattaagcat
    24241 gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa ccgtgagttc cggcgtcaaa
    24301 atgggtatag cgcccttcca cattaaccat aaagcggaca taaccgaggt aacgtgcttc
    24361 agtggcggcg tcaaagccga ggaaggtgac acgacgttca tcgatggatt gctggtcctg
    24421 caaattagac caggaaacat gcaacgcatg atacatctcc ataatgtcga tgatagtgcg
    24481 gcaggtttct tctttcagct cgccaaactc gcgatccaat tcacgcatct gtaatccgta
    24541 acctcgctca ataattgttt gcaggcgacg gtaacgttca gcatttgccg gatcgagcat
    24601 agtcatcatc ttgtactgat tagacaaaat aagacgttgc gcgttggtca tttccattgt
    24661 tgactcctgt atcactctac tacggtgaaa aaaaagaagg ctgagtatgc cttcttttat
    24721 atgcgtaatc aggggtcaat tacaaatcat caaggaaagt tttatccagt tgtttgaagg
    24781 cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt gccttcaacg ggtgccaaca
    24841 cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa ccagttaagg attgcagggg
    24901 gtaatggcgt tacagaacgc ttgcccagcc accacaatcc ctgcatgggt aaacttaagg
    24961 cgaacagtgc agtggcaact gccggcccaa gctgaccgcc cagggcaatc tgccagcaga
    25021 gagtaaatac ggcgatcggc ggcataaaac ggatcgcata acgcgtcatc ttgataacgc
    25081 gattttcgac aaagaccggg gcaaggcgtt tttccagtgg ccacgtcttt gagtaatgct
    25141 gtccgcggcg aaacaagcta aaaaaattaa cagaacgatt atccggcgtt gacatgcttc
    25201 acctcaactt cacatataaa gattcaaaaa tttgtgcaaa ttcacaactc agcgggacaa
    25261 cgttcaaaac attttgtctt ccatacccac tatcaggtat cctttagcag cctgaaggcc
    25321 taagtagtac atattcattg agtcgtcaaa ttcatataca ttatgccatt ggctgaaaat
    25381 tacgcaaaat ggcatagact caagatattt cttccatcat gcaaaaaaaa tttgcagtgc
    25441 atgatgttaa tcataaatgt cggtgtcatc atgcgctacg ctctatggct ccctgacgtt
    25501 tttttagcca cgtatcaatt ataggtactt ccatgtcgag taagttagta ctggttctga
    25561 actgcggtag ttcttcactg aaatttgcca tcatcgatgc agtaaatggt gaagagtacc
    25621 tttctggttt agccgaatgt ttccacctgc ctgaagcacg tatcaaatgg aaaatggacg
    25681 gcaataaaca ggaagcggct ttaggtgcag gcgccgctca cagcgaagcg ctcaacttta
    25741 tcgttaatac tattctggca caaaaaccag aactgtctgc gcagctgact gctatcggtc
    25801 accgtatcgt acacggcggc gaaaagtata ccagctccgt agtgatcgat gagtctgtta
    25861 ttcagggtat caaagatgca gcttcttttg caccgctgca caacccggct cacctgatcg
    25921 gtatcgaaga agctctgaaa tctttcccac agctgaaaga caaaaacgtt gctgtatttg
    25981 acaccgcgtt ccaccagact atgccggaag agtcttacct ctacgccctg ccttacaacc
    26041 tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg caccagccac ttctatgtaa
    26101 cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga actgaacatc atcacctgcc
    26161 acctgggcaa cggtggttcc gtttctgcta tccgcaacgg taaatgcgtt gacacctcta
    26221 tgggcctgac cccgctggaa ggtctggtca tgggtacccg ttctggtgat atcgatccgg
    26281 cgatcatctt ccacctgcac gacaccctgg gcatgagcgt tgacgcaatc aacaaactgc
    26341 tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac cagcgactgc cgctatgttg
    26401 aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat ggacgtttac tgccaccgcc
    26461 tggcgaaata catcggtgcc tacactgcgc tgatggatgg tcgtctggac gctgttgtat
    26521 tcactggtgg tatcggtgaa aatgccgcga tggttcgtga actgtctctg ggcaaactgg
    26581 gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc tgcacgtttc ggcaaatctg
    26641 gtttcatcaa caaagaaggt acccgtcctg cggtggttat cccaaccaac gaagaactgg
    26701 ttatcgcgca agacgcgagc cgcctgactg cctgatttca caccgccagc tcagctggcg
    26761 gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga ggataaaccg tgtcccgtat
    26821 tattatgctg atccctaccg gaaccagcgt cggtctgacc agcgtcagcc ttggcgtgat
    26881 ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc aaacctatcg ctcagccgcg
    26941 taccggtggc gatgcgcccg atcagactac gactatcgtg cgtgcgaact cttccaccac
    27001 gacggccgct gaaccgctga aaatgagcta cgttgaaggt ctgctttcca gcaatcagaa
    27061 agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct aacaccaaag acgctgaagt
    27121 cgttctggtt gaaggtctgg tcccgacacg taagcaccag tttgcccagt ctctgaacta
    27181 cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt atgtctcagg gcactgacac
    27241 cccggaacag ctgaaagagc gtatcgaact gacccgcaac agcttcggcg gtgccaaaaa
    27301 caccaacatc accggcgtta tcgttaacaa actgaacgca ccggttgatg aacagggtcg
    27361 tactcgcccg gatctgtccg agattttcga cgactcttcc aaagctaaag taaacaatgt
    27421 tgatccggcg aagctgcaag aatccagccc tctgccggtt ctcggcgctg tgccgtggag
    27481 ctttgacctg atcgcgactc gtgcgatcga tatggctcgc cacctgaatg cgaccatcat
    27541 caacgaaggc gacatcaata ctcgccgcgt taaatccgtc actttctgcg cacgcagcat
    27601 tccgcacatg ctggagcact tccgtgccgg ttctctgctg gtgacttccg cagaccgtcc
    27661 tgacgtgctg gtggccgctt gcctggcagc catgaacggc gtagaaatcg gtgccctgct
    27721 gctgactggc ggctacgaaa tggacgcgcg catttctaaa ctgtgcgaac gtgctttcgc
    27781 taccggcctg ccggtattta tggtgaacac caacacctgg cagacctctc tgagcctgca
    27841 gagcttcaac ctggaagttc cggttgacga tcacgaacgt atcgagaaag ttcaggaata
    27901 cgttgctaac tacatcaacg ctgactggat cgaatctctg actgccactt ctgagcgcag
    27961 ccgtcgtctg tctccgcctg cgttccgtta tcagctgact gaacttgcgc gcaaagcggg
    28021 caaacgtatc gtactgccgg aaggtgacga accgcgtacc gttaaagcag ccgctatctg
    28081 tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat ccggcagaga tcaaccgtgt
    28141 tgcagcgtct cagggtgtag aactgggtgc agggattgaa atcgttgatc cagaagtggt
    28201 tcgcgaaagc tatgttggtc gtctggtcga actgcgtaag aacaaaggca tgaccgaaac
    28261 cgttgcccgc gaacagctgg aagacaacgt ggtgctcggt acgctgatgc tggaacagga
    28321 tgaagttgat ggtctggttt ccggtgctgt tcacactacc gcaaacacca tccgtccgcc
    28381 gctgcagctg atcaaaactg caccgggcag ctccctggta tcttccgtgt tcttcatgct
    28441 gctgccggaa caggtttacg tttacggtga ctgtgcgatc aacccggatc cgaccgctga
    28501 acagctggca gaaatcgcga ttcagtccgc tgattccgct gcggccttcg gtatcgaacc
    28561 gcgcgttgct atgctctcct actccaccgg tacttctggt gcaggtagcg acgtagaaaa
    28621 agttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct gacctgatga tcgacggtcc
    28681 gctgcagtac gacgctgcgg taatggctga cgttgcgaaa tccaaagcgc cgaactctcc
    28741 ggttgcaggt cgcgctaccg tgtttatctt cccggatctg aacaccggta acaccaccta
    28801 caaagcggta cagcgttctg ccgacctgat ctccatcggg ccgatgctgc agggtatgcg
    28861 caagccggtt aacgacctgt cccgtggcgc actggttgac gatatcgtct acaccatcgc
    28921 gctgactgcg attcagtctg cacagcagca gtaacctcgt catcatccgc agctttgcgc
    28981 tgcggatatc tgaaccggaa ataatcacta tttccggttt tttattctct taatctgcat
    29041 taatcctttc tgattatctt gcttaactgc gctgcatcaa tgaattgcgc catttcactt
    29101 tacatactta ccactttgtt ttgtgcaagg gaatatttgc gctatgtccg caatcactga
    29161 atccaaacca acaagaagat gggcaatgcc cgatacgttg gtgattatct tttttgttgc
    29221 tattttaacc agccttgcca cctgggtagt tccggtgggg atgtttgaca gtcaggaagt
    29281 gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc gtagatccac actcatttcg
    29341 cattctgact aacgaagcag gcgaacctga gtatcaccgc gtacagctgt tcacgacggg
    29401 cgatgaacgc ccgggcctga tgaacttccc gtttgaagga ttaacctcag gatcgaaata
    29461 cgggacagct gttggcatca tcatgtttat gctggtgatt ggcggcgcgt ttggcattgt
    29521 gatgcgtaca ggaaccattg ataacggtat cctggcgctt attcgccata cccgcgggaa
    29581 tgaaattctc tttattcctg cgctgtttat tctgttttca cttggcggcg cggtatttgg
    29641 tatgggagaa gaggccgtcg cctttgccat tatcatcgca ccgctaatgg tccggctggg
    29701 ctatgacagt attaccaccg tcctggtgac ctatattgcc acgcaaatcg gttttgccag
    29761 ttcgtggatg aacccgtttt gtgtggtcgt tgctcagggg attgccggcg ttccggtgct
    29821 ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc actatgattg gcctgatctt
    29881 taccatggtg tacgcctcac gagtgaaaaa gaatcctctt ctgtcacgcg tgcatgagtc
    29941 cgaccgcttc tttcgtgaaa agcaggcgga tgttgaacaa cgtccgttta cctttggtga
    30001 ctggctggta ttgattgtcc tgaccgccgt aatggtctgg gtgatttggg gcgtgatcgt
    30061 taatgcctgg tttattccag aaattgccag ccagttcttc accatgggtc tggtgattgg
    30121 catcatcggc gttgttttcc gccttaacgg catgacggtt aataccatgg cttcatcctt
    30181 taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg gtgggtttcg ccaaagggat
    30241 tttgctgctg gtcggtaatg gtgaagcggg tgatgccagc gtgttaaata ccatcctcaa
    30301 cagcattgcc aatgccatta gcggtctgga caacgcggtc gcggcctggt ttatgttgct
    30361 cttccaggcg gtatttaatt tcttcgtgac gtccggttct ggtcaggcgg cgttaaccat
    30421 gccgttactg gcaccgcttg gcgatctggt cggtgttaac cgtcaggtta ccgtgctggc
    30481 attccagttt ggtgatggct tcagtcacat catttaccca acctcagctt cgttaatggc
    30541 aactctcggt gtgtgcaggg tggacttccg taactggctg aaggtgggcg cgaccctact
    30601 tggactgctg tttattatgt ccagcgtcgt agtgatcggc gctcagttga tgggctacca
    30661 ctaaaaatgt taagagccgc aattgcggct cttttttcat tctgccgttt cagtctctac
    30721 ggcttcattt ttggcattgc gcttcatcca caacgccagc gctttcagcg agtctggagt
    30781 gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc cagcaaactt cactgacttc
    30841 atcttcctgt agggcgaagg gaccgtgaga gacgcagctg aacaacgcgc cccagacacg
    30901 gcaattttta tcttcgaaat agaactgccc gtgctcggca aaggggacac cggcaatgcc
    30961 caactcttct tccgcttcgc gacgcgcgga ttccagcagt tgctcatcgg cctgaaccac
    31021 accgcctgcg gtcgcatcta acatgccggg taaaaagtct tttgtctcgg tacgacgctg
    31081 gaccagaatt ttgcccatgc catcatgcac gacgatgtaa gttgcacgat gacgcagaca
    31141 ctgtgcccgc atttgttccc ggctggcttg tgcaatgact tcgttctctt cattgacaat
    31201 atccacccat tcagtacttg ccaaacgacg ctgttccatt atcaggaaac cttctttttc
    31261 tggcgctctt acggcgcatt ttgagttgtg ggtaaattac ggattaatcg cgacctgcgc
    31321 aatgatactt tgatcattga gtgcgattac actaagtacg tcattatcca gcatgccata
    31381 actcgccgga ttaccgcctt tcggaatact caccgagccg gggttgaagt ggaaaatctc
    31441 cccccgctgt tctgccactg gcagatgggt atgaccgtac accagcacat cgttctggtt
    31501 taaagcaggt agattttccg ggccaaaaag atgaccatgc gtcaaaaaca gacgttgttt
    31561 ttccagtaat acctgttgcc acggcgcggt tatcgggaaa tgcagcagca tttgatccac
    31621 ttcgctgtcg cagttgccgc gcacagcgat aaccttatgt gccacttcat taagccgttc
    31681 agcgactttg gctggcgcgt aaccctccgg taaagcatta cgcgggccgt gattcaacac
    31741 gtcgccaagg atcaccagcc actgggcacc actttgggca aacaactcca gaacacgttc
    31801 cgtcgccggt aacgacccat gaatgtccga tgcaaacatc agtttcatca ctcactcctc
    31861 gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg ctatcagcca gattgcttcg
    31921 ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca ctggaatgtc gcataatcca
    31981 ccaggcgttc tggcacctca tcgccatgta gtaccaggcg gttaatcatt agcgccagat
    32041 cagtatcagc aatgcaccat tcaccaaata aattcggctg acccagtgct aacaaatgtt
    32101 ctgccatcgc gaacagtttc tctgcactgg cttttccctc ggccgttagt ggcgcttttt
    32161 tcgcccccgc aaagacaaca tccgtcggac gctcttcgcg gatgggcatc agatcgctgc
    32221 gcagccaggc ctgaatccgt cgtgcacgcg cacgattttc taaatcaagc ggataaatac
    32281 gttcccaggt cggtggcgca aatcgatctt ccagatactc cgcaatggca gaagattcac
    32341 tcaactcaaa atcatcgatt tgtaataacg gcacacggcg tgtctgaccg taaccttgcc
    32401 acgtcggctt caaatgttca ccgctgtcga ggtcgatggt cttgatatga aacgacaggc
    32461 ctttttcctg caacgccacc caggcggata acacataagg ggagaaaaag tgggcatctg
    32521 accaaagcgt gatagcgggt ttactcatta tgtcctcggc aataaagggg gctggctatc
    32581 aaactataga gcctttttgt cactgtcacg tgacgaatac tcacgtaagt cgccgcgctt
    32641 tctatacttg tggtgttcag atgattaaac aaccggagct gcaatgatcg atctctattt
    32701 cgccccgaca cccaatggtc acaaaattac gctgtttctc gaagaagcag agcttgatta
    32761 tcgcttgatt aaggtagacc tggggaaagg cggtcagttt cgtccggaat ttttgcgcat
    32821 ttcgcccaac aacaaaattc cggcaattgt tgatcattct cctgccgatg gcggcgaacc
    32881 gctaagcctc tttgagtctg gtgccatttt gttgtatctg gctgagaaaa caggactctt
    32941 tttgagtcat gaaacgcgtg agcgcgccgc cacattacag tggttattct ggcaggtagg
    33001 cggactgggg ccgatgcttg ggcaaaatca tcattttaat cacgcagccc cccaaaccat
    33061 tccttacgct attgaacgtt atcaggttga aactcagcgt ctttaccatg tactaaacaa
    33121 gcggctggaa aactcgccct ggctgggagg cgagaactac agcattgcgg atattgcctg
    33181 ctggccgtgg gttaatgcct ggactcgcca gcgaattgac ctcgcaatgt atccggcagt
    33241 caagaactgg catgagcgga tccgttcgcg ccctgccacc gggcaggcac tgctaaaagc
    33301 acaactcggt gatgagcgtt cggatagtta acagaaacag gttctcgtgt attatttcat
    33361 cctaagtaaa acaacggaga acctgcaatg gcacaacctg ccgctattat tcgtataaag
    33421 aaccttcgtt tacgtacgtt tatcggaatt aaggaagaag aaattaacaa ccgtcaggat
    33481 attgttatca atgtgacgat ccactacccc gccgataaag cgcgcactag cgaagatatc
    33541 aacgatgcgc tgaattatcg caccgtaacg aaaaacatta ttcagcatgt agagaataac
    33601 cgtttctctt tgttggaaaa attaactcag gatgtgctcg atatcgcacg tgaacatcac
    33661 tgggtgacgt atgctgaagt ggagatcgat aaactgcacg cgctgcgcta cgccgattcg
    33721 gtatccatga ccttaagctg gcagcgttaa ccgccatatc gggaggctgc atgaacatag
    33781 tgatcaccgg agggacggga ttaattggtc gccatttgat tccgcgtttg ctggagctgg
    33841 gccatcaaat cacggtagtg acgcgtaacc cacagaaagc cagttccgtt ctcggccctc
    33901 gggtgacgct atggcaaggg ctagccgatc aaagcaacct caacggcgtt gatgcggtaa
    33961 tcaacctggc cggagaaccg attgctgata aacgctggac tcacgagcaa aaagagcgtc
    34021 tctgccaaag ccgctggaat atcacgcaaa aactggtcga tttgattaat gccagcgaca
    34081 cgccgccgtc ggtactcatt tccggctcgg caacgggcta ttatggcgac ttaggtgaag
    34141 tggtggttac cgaagaggaa ccgccgcata acgaatttac tcataaactc tgcgcccgct
    34201 gggaagagat tgcctgccgg gcgcaaagtg acaaaacgcg agtgtgcctg ctgcgaaccg
    34261 gcgtggtact ggcaccggac ggcggtattc tcggtaaaat gctgccaccg tttcgtcttg
    34321 gcctgggcgg gccgattggt tccggtcggc agtatctggc ctggattcat attgatgata
    34381 tggtcaacgg cattctctgg ctgctggata acgacctgcg cgggccattt aatatggttt
    34441 cgccctaccc ggtacgcaat gaacaatttg cccatgcgct cggtcaagcg ctgcatcgcc
    34501 cggccatttt gcgcgtcccc gcaaccgcca ttcggctgtt aatgggcgaa tcttcaatac
    34561 tggtattagg tggacaacgc gcgctgccta aacggctgga agaagcgggt tttgcgtttc
    34621 gctggtacga tttagaagag gcgctggcgg atgtcgttcg ctgatgtggt ttacagcaaa
    34681 catccgccag ttaactcccg gtgttacagg attagtggct ttgcgcgata agatcgtctg
    34741 gtgaaagtcg ggtcaccatc ataactaact ctctgtctaa acctctatcc agcatctcct
    34801 gagcaatacg cagggcttct tcgtgtttgc cctgcattgc gccttcttca cgtaatctgt
    34861 cagcaatggt catcagtttc tccttttctt gtggtgcgcg ttctgttatc tcaccaataa
    34921 atgcacggaa acgctgggca tcccctgttt gtaatacgta attaaacagg gcttttagct
    34981 gtctgtcatt agtgttccct gtaactagca gcgaaacaat ttggtcgact aatcccaaca
    35041 gatcgcgctg acgaatatgt ttctgaatta actccaacag cgccattttg cggtgttgca
    35101 taatctcgtc atccggcacc acggtaatat ccaccaacgg aaacgctgat gaatatattt
    35161 tgcgggctat agcaggctcg gcaaattcat caagccagca gagtgaataa ggataaggac
    35221 ttctgcaacc atgataaaac agcatcggga tcaccaatgg aagctcttta tagcccgcat
    35281 caagatggtt ttgcattgcc gcaatggaat aacgcatcat gcgaaaagcc attaattctt
    35341 ccagcttact ttggtgctct atcactacat aaatataacc cactccctcc tgcgttttca
    35401 cagaccacaa gaggtcggaa taatattgcc gcaggtcttc atcaataaaa ctgtttgatt
    35461 ctagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg cgcgggaaga tgaatatcaa
    35521 taaaatcccg cgcggtgtct ggatggcgta aaaaagattt aaataccgca tcatgcggcg
    35581 tggaagttgt cgatattgtc atggcgttcc gtcacccctc aaatttaaga tgacgcgaca
    35641 ataaccacga ttaacaccca atgcagcagg cagtttttat ctttggacag cgaattcaga
    35701 agtattttta gcggactgca acttttacat ttcagcggaa gctgactcgc aaattcccca
    35761 tgaaaatact ccggcagcgt tagccaccgg agaggaaatt atttcagcga tcccttaagg
    35821 aactgttgca gacgagggct ttgcgggttg ccaaataact gctccggcgc gccctcttct
    35881 tctattttcc cctgatggag gaaaatgacg tgagtagaaa catggcgagc aaagcccatt
    35941 tcgtgagtca ctaccaccat ggttttcccc tcttctgcca gttgctgcat aatacgtaac
    36001 acttcgccta ccagttcagg atcgagcgcc gaggtaggtt catcaaacag taaaacttcc
    36061 ggttccatcg ccagcgcccg cgcgatagaa acacgctgtt gctgaccgcc ggaaagatgc
    36121 accggatatt tcccctgcgc acgttcgtct atcccgactt tcgccagata cttcaccgcc
    36181 cgctcgcgcg cttcctgctt gctcaggccc aacacctgaa tcggcgcttc catgacgttt
    36241 tccagcaccg tcatatggct ccagagattg aagtgctgga ataccatcgt caggcgtgtg
    36301 cgcagtaagc gcagttgatt tttatcggcg actttgagtt gaccgtcttt gtcgcgcacc
    36361 agattgatcg tctggccatt gaccacgatc gacccttcac tcggtttttc gaggaagtta
    36421 atgcagcgca gaaaggtact tttccccgat cccgacgatc cgatgatgct tattacatct
    36481 ccggcattcg cttgcagtga gacccctttc agcacttcat gttcgccgta gcgtttgtgc
    36541 aaatcgataa cgtttaattt attctcggac atcgtattct cagtgcgttg aagaaggttt
    36601 cacatgctgc aaccagcgtt tttccgctct gcgaaagagg ctgatcagga cataagagat
    36661 gattaaatag agcaccgcgg caatgccgaa ggcggtaaaa ggttgatacg tggcggcgtt
    36721 aatatcgcgg gctattttca gcagatccgg cacagtggcc gtaaacgcca gcgcagtaga
    36781 gtgcagcatc aggatcactt cgttgctgta tgccggtaac gcaatacgca gcgcagaagg
    36841 caaaataatg caacgataca ttttaaaagt cgagaagcca taggctctgg cggcttcaat
    36901 ttccccatgc ggaaccgaac ggattgcccc agcaaaaatc tcggtagtgt aagcgcaggt
    36961 gttaagcgtc agcgccagca cggtacagtt caggccactg cggaagaaag cgttaaggaa
    37021 ttcggttccc ttaacaatct caagcgtgta catgccggaa tagaacacca gcaactgaac
    37081 atacagcggc gtaccgcgaa aaatataggt aaataaccag attggaaact ggatgtattt
    37141 attactggag acacgaccaa tcgccagaaa cagcgccagg actccgccta tcactaccga
    37201 caaaataagc agccacagag tgatcgccac accagtaaag cgataaccgt cggtccacag
    37261 cagcggtttc cagtattcat gtaagatttc gatcacaggt cagccctctt cacacccacg
    37321 gagtagcggc gctcaaggaa cagcagcaca ccattggaaa cagtggtgaa aaccaggtaa
    37381 atcacgccac agacgatggc gaaatagaac ggttcccagg tacttttgcc tgccagttgg
    37441 gtggctttga ccacatcttc caggccgagt aacgaaacca gtgcggtaga tttgaggatc
    37501 acctgccagt tgttgccaat gcctggcagg gcgtaacgca tcatcgccgg aaacatgatc
    37561 cgccgaaaca cttgcccacg agtaaaaccg aacgccgtcg ccgcctctat atgtcctttc
    37621 ggcactgcca taaaagcacc acggaacgtt tcggtaaaat aagcaccgta gataaaaccg
    37681 agagtgataa taccagcgac catcggatcg atatcaatct gcccgacgcc catcgcctcc
    37741 gtcaccgtgt ttagcgcaat ctgcaaaccg tagaaaatca gcagcatcaa cactaagtcc
    37801 ggcacgccac gaatcagcgt ggtgtaccct tcgaaaatca gccccgaaag ccgattttgc
    37861 gagagcttac cgccagcgcc aattaaaccg atgattacag cgagcactac agagctgata
    37921 gccagctcca gcgtgacgag cgcaccctgt aaaataacac ctgaaaaccc atacaacatg
    37981 ctgcctgtcc tgtcgtgtgt ggtgaattac cgcctcttgt ctccctccgg ttacccgaag
    38041 ggagcgggct ttctttcacc gatggcgatt agccaccata aacatcaaaa tcgaagtact
    38101 ttttcgctaa tttctcgtaa gtaccgtcag cgcgcatttc ggcaaaggct ttgttcagtg
    38161 cttcgcgcag ttcgttatct tctttacgca ggcccatgcc ggtccctacg ccaaacagtt
    38221 tttcatcttt aacagacggg ccaccgaatt tgtaatcttt accgacaggt tgtttgagga
    38281 aaccttcgct ggcagcgacc tcatcctgga acgcggcatc aatacgtccg gcagtcaggt
    38341 cagaataaat gttgtcctgc ccctgatacg agacgatttc aatgcctttt ggtgcccaat
    38401 gttcattacc gaacgtctcc tgggtggtgc cctgcaatac gcctacccgt ttgcctttca
    38461 gcgactcgac tgtcggctga atgtcagaat ttttcgccac caccaaacga gaatcggcag
    38521 cgtacagttt gtcggtgaag gctatttctt gctgacgttt ttccgtaatg gaaagcgatg
    38581 acatgatggc gtcaatcttc ttcgctttta aggacgggat taacgcatcc agcggatttt
    38641 cgacaaacgt acattgcgta ttgatgcgtt tgcataattc ctttgccaga tcgatatcga
    38701 agccaaccag ttcgccttgt gaattttttg attcaaatgg cgcataggtc gggtcggtac
    38761 cgatgcggat gttttgcgga atcgcagcaa atgccgcagt tgcgctggag aaggccagaa
    38821 ccagagagag cgatagcacc agttttttca taatgtcctc aactgacagt ctttttatag
    38881 ggaatattta caggtttgtt acacttatcg tgccataaaa ttgaccatca aggcaaaata
    38941 ttccgccccg ttagacgtat tatttttcat taaaaatgct taagtatgca tttcaatgca
    39001 ccacgatagt gcaacaaacc tggcgtgcac catgatgatc attccatcag gtacagcttc
    39061 ccagcgacgt atcagtcacc gtagacatta aagtcgaaat actttttcgc catcttgtcg
    39121 taggtgccgt cctgacgcag ctcgccaagc gccttattga aggcagccgt cagttcagca
    39181 tcatctttac gtagccctac accggtgcca tcaccgaagt attttttgtc ttttactgat
    39241 gagccagcaa aggcgaaagc tttaccagca ggttgcttga ggaatccttc gctggcagca
    39301 acttcatctt gtaacgcagc atccagacgt cctgcagcca gatcggaata gaccaaatcc
    39361 tggttggcat aggccaccac atccacgcct ttgctacgcc aggtctcgtt agcgtaagct
    39421 tcctgggttg atccctgcag cacaccaaca tgtttacctt tcagtgaatc cagcgttggc
    39481 tgaatcggtg aacctttggc agcaatcaaa cgagaatctg cggcgtacag cttgtcggag
    39541 aaggcaatct cctgctgacg tttatcggta atggaaagcg acgaaataat ggcgtcgatt
    39601 tttttcgctt tcagtgaggg gatcagcgcg tcaaagtcac tggcaaccca ggtacatttc
    39661 acctgcatcc gtttgcacat ctcgttaccg agatcgatat caaagccaac aaaatcacct
    39721 ttagcatctt tcgacgagaa cggcgcatag gtggtatcgg ttccgatacg taccgtctcc
    39781 ggtagcgccg catagctgga agccgctgcg gagagaccga ctaacaaaga cagagcgaga
    39841 atcgacttct tcatacataa ccctcaagtg aaaatggctt ttttatgttg tgtattgtgt
    39901 tgtgtgtttg caggctcttt catgcaggtc ttatgccatc ttgacgacaa cagtaacatt
    39961 caacgttaaa tatgttaata agacgttgca ttattgtcct gaagttgaag atagcaggta
    40021 tagcggttga atcgcagcgt ttcgattgaa tggcagaaac aaaatgtcga ggatttgatc
    40081 gcggttgcaa aattgccctg aaacagggca acagcggagt tatgcgccct gccagcgggc
    40141 aaagagatct tcaggaaggg ttattgcaaa ctggtcaaga acacgattaa ccgtctgatt
    40201 tatcacatca tcaagggatt gcgggcgatg ataaaacgcc ggaacgggag gcataatcac
    40261 cgcaccgatt tctgccgcct gagtcattaa acgcagatgg cctaagtgca atggtgtttc
    40321 acgcacgcag agcaccaacg ggcgacgctc tttcagcacc acatctgccg cacgggtcag
    40381 taagccatca gtatagctat ggacaatgcc ggaaagggtt ttgattgaac agggtaaaat
    40441 caccatcccc agcgtctgga aagaaccgga agagatgctg gcggaaatat cgcgcgcatc
    40501 gtgcgtgaca tcagctaatg cctgcacttc gcgcagggaa aaatccgttt cgagggataa
    40561 ggtctggcgc gccgcctggc tcatcaccag atgcgtttcg atatctgtga catcgcgcag
    40621 aacctgtaat aagcgcacgc cataaatcgc gccgctggca ccgctgatgc ctacaatgag
    40681 tcgtttcata aatagttgcc ctgtcagact tgcaggcaga ctttgcagta tttcggcggg
    40741 agttgcaagt cagggtgcca gaccggcacc ctcagcgaag gcatcatcct tcgttatgca
    40801 tttcgagatt ttccacttcg ttctgacgtt gcactgcttt ggcgtcatca ttacgtaacg
    40861 tatcgaggaa atcgaggtag ccctgatcaa catctttggt gacgtagacg ccgttgaaca
    40921 ccgagcattc aaactgctgg atatccggat tttcagcgcg aacggcgtcg atcagatcgt
    40981 tcagatcctg gaaaatcaac ccgtcagcac cgatgatctg gcgaatttca tcaacttcgc
    41041 gaccgtgagc gatcagttcc gtggcgctcg gcatatcaat accataaacg ttcgggaagc
    41101 gaatttccgg tgccgcagaa gcgaggtaca ctttcttcgc tccggcttcg cgtgccatct
    41161 cgataatctg ctcagaagtg gtgccacgga cgatggagtc gtcgaccagc aggacgtttt
    41221 tatcgcggaa ctcggcgcgg ttggcattca gtttacggcg cacggactta cgacgcagct
    41281 gctggcccgg catgataaag gtgcggccaa catagcggtt tttaacgaag ccctggcggt
    41341 acggtttgcc cagaatacga gcaatttcca gcgcgatatc acacgaggtt tctgggatcg
    41401 ggatcaccac gtcgatatcc agatcttccc attcgcgggc aattttctcg cccagtttcg
    41461 tgcccatatt cacacgcgcg ctgtaaacgg aaattttgtc gatgaacgag tccgggcgag
    41521 caaagtatac atactcaaac aggcacggat tgctgaccgg attgtcagca cattgacggg
    41581 taaacaactg cccttcttca gtgatgtaaa tcgcttcgcc cggcgcgacg tcacgcagga
    41641 aatcaaagcc cagcgtatcg agcgctacgc tttcggaagc gaccatatat tctgtacggt
    41701 tctcgtcaat atcacgtttt cccagtacca gcggacgaat cccgtttgga tcgcggaaag
    41761 caaccatacc gtggccgata atcatcgcca cacaggcata tgcgccgcgg attaagcggt
    41821 ttgtggcagc aatggcagcg aaaatattgt cggcttccag cgggtagtgg cggaagttgt
    41881 ccagctcgct agcgaagata ttaagcagaa tttccgagtc ggaagtggtg ttgatgtggc
    41941 ggcgtttttc ttcaaacagt tttttacgca actcgtgagc gttggtcaga ttgccgttgt
    42001 gggcaagcgt aatgccatac ggggagttaa cgtaaaacgg ctgcgcttca gaggcgctgg
    42061 agctgccagc cgtagggtaa cgcacatgac caatgcccat attgccctgc aaacgctgca
    42121 tatggcgagc ttcaaataca tcgctcacca gcccgttcgc tttacgcaaa cggaagcagt
    42181 tattggcatc tatggtgatg atgccggcgg catcctgacc gcgatgctga agcaccgtta
    42241 aggcatcata aatcgactgg ttaaccggca taacaccggc gataccgaca ataccgcaca
    42301 tacgtctttt tcctcgttaa gccacatctc agagcactta cgctctgggc aagaaacttg
    42361 acgagctttg cagataatca aaaaaccatc tgatgataaa actgaactgc gggatcagct
    42421 gtgatttgct ccagtcttcg cttttcgaca ccccggtaaa ggagtcgaga aagaagagaa
    42481 tggcggcaac aatcaacaca ccgcgcaacg caccgaaaca gacgcccagc acccgatcgg
    42541 tgcctgacaa ccccgttttc tccaccaact ggcctatcac gaagttcacg atagcaccaa
    42601 caatcagggt agcgataaac agtaccgcga tggcaattcc atttcgaacc agttcgtctt
    42661 caaagcccgt aaaccagact gacaggtaag tgtagtaatg actggcaaca aagaaagcac
    42721 aaccccatgt caccagcgat aacgcttcac gaacaaagcc gcggatcagg ctaaccagag
    42781 aggaaaaagc aatcaccgcg attatggcgt aatcaatcca gaccatatgt gtcccacgat
    42841 tttacgccct gtcatcctgt tcggggcgca ttctaacaga aaaagaaaac gtttgcgtag
    42901 ggatttcctt cccacgcatc aataaaaatg gcgctgaaaa aatattcaac gccatcgact
    42961 ttttatgcct ttgcggcatc gtgcaatgcg tgtcggatgc ggcgtaaacg ccttatccga
    43021 cctacggttc taacactgcc caggcctgat aagacgcgcc agcgtcgcat caggcaagac
    43081 tgtattaatt cggcgtatag cccattacca cgccacttaa gccagaaagt tgcttcaact
    43141 cacccagcga acctttcagc ttatctttcg aggcatccgg cccaaccaga atacgggtaa
    43201 ttttaccctg cactggcgtg gatggcgacg tataaacccg ataaccggca ccgcgcagct
    43261 taccgacaat ctcattcact ttatcggcat ttttcagcgc acccagttgc acaacatagg
    43321 ctttacccgt tggcgctgct ttttcttcca cgaccggctt cggttctggc ttcggcgcag
    43381 gtggtgcttc aaccttcggt ttaggcggct ccaccggttt cggcttcggt ggggcgaccg
    43441 gtgcaggttc cggttcaaac tcggtgttat tggctgcaat agtggccgga tcgagcgacg
    43501 gtgctgcggc atcacctgcc cgcacctctt ccgctgcgcc ttccggcggc tgcgtcggca
    43561 acgcctgggt ggcggctggc atcatatcag gctcatcgcg atcgcccgct tttggaacca
    43621 gcgggatagc cgcaaactca tcctgataat gttttttctg cccgtccagc agccctggaa
    43681 gtacaatcac ccccagcgcc accagcacga tcgtgcccac taaccgattc tgaaacttac
    43741 ttgccaccgc ttctcctcgc gtcaatcact tccatgacat gtgcgaccgt gtggaaagaa
    43801 ccacacacca gcacggtgtc ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc
    43861 gcaacgctat caaatgattt gccgttaccc aaatgctcaa gcagttgttc tgccgtggca
    43921 ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa ccacgctttt caaccaggcc
    43981 agagttccgg caatatcttt atcatgtagc ataccgataa ccgccagcac gcgcccgttt
    44041 ttcggtagcg ctttcatacg cccggtgaga tattccgccg catgtggatt atgcgcgaca
    44101 tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac gtcccggcaa aattgcgctg
    44161 gcaatcccgt cgcgaatggc attttcactg acttccagcc cgctggcacg cagtgccgcc
    44221 agcgctgttg cggcattcgg ttgcgggaca agcggcaacg gcagattttc cagcgtgccg
    44281 tgagcatcgc taaacgccca gtcatgatcg gtgacggaat agttccactc aacgccccga
    44341 cgttgtaaca gtgcaccttt ttcctgcgcc acatcagcaa tggtagaagg catttccggc
    44401 tcaccgacaa ttgccggttt tgcgctgcga aagatgcctg ctttctcgcg accaatactt
    44461 tcgcgatctg gtcccagcca gtcggtatga tccagcgcaa tgctggttac tacagccaca
    44521 tcggcatcga caatattggt tgcgtccaga cgaccgccca gccctacttc cagaatcact
    44581 acgtcaagtt gtgcctgttt gaacagccac aacgccgaca gcgtaccgta ctcgaaatag
    44641 gtcagggaaa tatcaccgcg tgccgattca atctccgcaa aagaggcggt gtgggccgat
    44701 tccggcaatt cctgcccctg cacacgtacg cgctcggtat aacgcaccag atgcggtgaa
    44761 ctgtagacgc ccactttgta ccctgccgcc atcagaatcg actccagcgt acggcaggtg
    44821 gtgcctttgc cattcgtacc cgcaacggta aacacaaatg gcgctggttt caggacgcca
    44881 agacgcgccg cgacctggct cacgcgctca aggccgagat cgatagtttt actgtgcagg
    44941 ttttccagat aagaaagcca cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata
    45001 atcatggtat ccgctgattc gttacggtga gaatagcaaa agggcagagc cagtggccct
    45061 gcccttatca gttatcaggc ctcaggttcc tgatccggta ccgggggtac cactacgcct
    45121 tcacgcggcg cttcaggatt cggcgctggc agattcatca acttcgccag aatgctcgcc
    45181 agtttcaggc gcatttccgg acgatggacg atcatgtcga tggcgccttt ctcgatcagg
    45241 aattcactgc gctggaatcc tggcggcagt ttttcgcgaa cggtctgttc gataacacgc
    45301 ggaccggcaa agccaattaa cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg
    45361 aaacttgcag aaacaccgcc catcgtcggg tcggtcagca cggagatgta cggcaagccg
    45421 cgctcctgca tttttgccag tgccgcagag gttttcgcca tctgcatcag cgacatcagt
    45481 gcttcctgca tacgtgcgcc accagaggcg gagaagcaga tcagcgggca gttatcttcc
    45541 agcgcctgct caacggcacg cacgaaacgt gcacccacaa cagaccccat tgaaccgccc
    45601 ataaaggcga actcgaatgc cgcagcgaca accggcattc catacagagt gcctttcatc
    45661 accaccagcg catctttttc gccggtttct ttctgcgcag aagccagacg gtctttatac
    45721 ttcttggagt cacgaaactt cagcacatct ttcggctcaa gctcgctacc cagctccaca
    45781 aggcttcctt catctaacag gctatgcagg cgattacgcg ctgtcatacg catgtgatgg
    45841 tcacacttcg gacagacctc aagattacgt tccagctcag cgcggtataa aacctgaccg
    45901 cagctatcac acttagtcca caccccttca ggaatgctcg ccttgcgggt gggagtaatg
    45961 ttgcttttaa ttcgttcaat ccagctcatt agggaccttt ctgtctgaac ctggttcgat
    46021 gccagtttta tctttgggga cgcataatgc catttttgcc cccaacagac catgaatgtt
    46081 gcacattaaa acataacagc ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta
    46141 ctttttattt tgcggcgcgt gccgcagcgc gtttgtgacg aataaattcg aaaaccccag
    46201 gcaatattga aacaaaaata atgccaacaa tcatcagctt aaggttgctc tgaatgaagg
    46261 ggagcgtgcc gaagaaataa cccgcgtagg taaaaatcag tacccacaac agtgcgccga
    46321 tcacgttata agcggcgaaa tgacggtacg acatgtggcc cattcccgca acaaacggcg
    46381 cgaacgttct gacgatcggt acaaaacggg cgagaataat cgttttgccg ccatgtctct
    46441 cgtaaaactg atgagtctta tcaagataac tgcgacgaaa gattttcgaa tccgggttac
    46501 tgaacagttt gtcaccaaat aaccgtccaa tggtatagtt tactgcatca ccgacaatag
    46561 cggcaatcaa cataagcacc accatcatat ggacattgag atcgttagtt tccagcgacg
    46621 ccaatgcacc agcaacaaac agcagcgaat caccaggcag gaatggcgtt accaccagcc
    46681 ccgtttcgca gaacagaatc aaaaacagaa tggcataaac ccagacgccg tactccgcga
    46741 ccagctctgc cagatgcacg tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt
    46801 ccatatttgc ctatgccttg tactcgtcat ctttcaggct gtaactacgt tggctacagc
    46861 ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa atagcgggcc cattggcggt
    46921 tttggaagat cataccggtc agggtaatcc accgcgacca gatacagccc ttccgctttt
    46981 gccgttgctg ccgccagcgt tctgtccttt gccgccagca actctgctat ccatctctcc
    47041 ggctggttgt gggcaccgac ttccatcagg ctgccgacaa tattcctgac catatgatgt
    47101 acaaaggcat tcgctttgat atctaccacc acataaggac cgtgacgcgt gacattaata
    47161 tgcataacgt tgcgccacgg ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa
    47221 tcattctcgc ccagcaagca ttgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg
    47281 taaaaatggg ttaccccttt actcaatacc gccgggcgca gccgatgatt gtagatgatg
    47341 tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa aatcatcagg tacagcttta
    47401 acccaacgca cggcgatgtc accaggtaaa ttcgcattta cgcccaaggt ccacgccgcg
    47461 tctttgcgct gcgcggttgt ttcgaaatgc accacctgcc cggtaccgtg tacccctgcg
    47521 tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg ccacctggga gagcgccttt
    47581 tccagcttct cctgcacact gcggacttca ttctgccgtt gccagccgta atacttactg
    47641 ccgtcgtact caatgcccag cgcaatttta taaactggca gttgttgctg gtcggacatt
    47701 agtacagata ctcctgcacc agtttctcgg cgatttttac tgccatcagc gcgccgccaa
    47761 agcgaacgtt atcggccacc gaccagaact ggacttgctc cggcataccg tagtcattac
    47821 gcacgcagcc aacagaaaga tgcggcgtac ccgaagcatc ccctacctga gtcgggaatt
    47881 cgttctcttc agagagcaca atatcttcgc cctgagcaaa cgcatcacgc gcttcttctg
    47941 ccgccagcgg acgcagtgct tcaaagttga ccatctgggc atgaccgtag aataccggtg
    48001 cctggacgac gcttgccgaa atcatcagcc cttcgtcctg caggattttg cgtacttcgt
    48061 caacgatacg acgttcttca cgcacgctac cttcgctatc cggcaacaac ggcagcatat
    48121 tgaacgccag ttgacgaccg aagaaatctt cttcgtcaat cggaatgccg ttgagcaatt
    48181 tcgcactctg ccccgctaac gcatcgaccg cttttttgcc ctgggcggag gctgaaatca
    48241 ggctggtaac actgatacgc gacaaaccgc cctgatcgat taacggtttc agtgccgcca
    48301 gcagctggct ggtcagactg tctggtacgg cgatgacatt ccggttgcgg taatcggtca
    48361 gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc gggttcgaga gcaaacagtc
    48421 cgctgctgtc gatcaccagg caacctgagt tggtcgcttc ttcaacccag gcagcggtag
    48481 cttctttgcc tgcgacaaaa aatgccagct gcgcctgcgt ccagtcgaat tcagcggcat
    48541 cctgcacagt gattgtttta ccaccaaagc gcagttgttc gcctgcgctt tcgttacgtg
    48601 ccagtgcata aatttcccca accgggaact gacgttcagc cagcgtttca agcagggctt
    48661 cgcccacagc gccagttgcg cccaggacgg caatgttcca gccttcagac atggtggttt
    48721 actccagaaa tagcaaagct ccctgccaat gttacagcag agagcatgaa gaagagatta
    48781 acgtgccgga tgatgaacgg cgttaaaacc cagtttacac agcaatgatg ccgcactggc
    48841 gtcatcacaa attacataca gagatgacca ttcacggcgc tcaagatagt ttttgcgcag
    48901 tttatcgaac tcacccggta tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac
    48961 atcatacacc aaatgcacca gccttttcag cgtcggttga tcgagcgggc catgcagcgt
    49021 aatgcgacca aactctggcg cgggcagtaa tgtatccagc gcaacgtgct gttcatgccc
    49081 aataaacttg ctataggctt caaacacttg cgtagtaccg cgtgctttac cttccagggt
    49141 ataacctgcg atatgcggcg tgccgatatc cacttttgtc agcaactcca cgttaagttc
    49201 cggttcgcct tcccagacat ccagcactac gcttaacttc tggccttcat tcaggcaggt
    49261 cagcaacgca gtattatcga cgactgcgcc acggcaggcg ttaatcagaa tcgctcccgg
    49321 cttcaggcta cggatcagtt tttcatccgc cagatgcagc gttttgtacg gcccatcttt
    49381 gaagagtggc gtatggaaag tcagaatatc cgcatgctga actaattcat ccagcgagcg
    49441 gaaatcaccc tcatccccac ggtcagcgcg aggcggatcg caaagcaatg ttttaatccc
    49501 taacgcttcc agtcgcgcct gcaatcgacg tccaacgtta ccaacgccca cgatccccac
    49561 cgtacggtcg tgcagtgaaa atccatcgcg ttcggcaagc atcagtagtg aggagaaaac
    49621 atattccacc accgcaatcg cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg
    49681 cttcaaccat gcttcatcga catggtcggt ccccgcagtg gcagtgccaa caaatttaat
    49741 gggttttcct gccagcaaag attcattcac tttcgtgacc gaacgcacca tcagcgcatc
    49801 cgcgtctgcc agttgagcga cggggattgg acgcccggga accgcggtca cctcacccaa
    49861 acggctaaat aagtcgcggg cataaggcat attttcatca acaaggattt tcacgtttgt
    49921 gttacctgta tgagacgaga gttaaccgga caagtgtgcc ataatctcgc ggccaggcat
    49981 acttgcgaag atttcaggta taaggatacg taatgataca acctatttcc ggccctcctc
    50041 ctgggcaacc accaggtcag ggagataacc tgccgtctgg cgcgggcaat cagcctttat
    50101 ccagtcagca acgtacttcg ctggaaagct taatgacgaa agtgacctca ctgacgcaac
    50161 aacaaagagc agaactgtgg gcgggtatca ggcacgatat tggtctgtcg ggagattcac
    50221 cgctgctttc gcgtcacttc cctgccgctg agcataatct ggcgcaacgt ctgctggccg
    50281 cgcaaaaaag ccattctgcc cgccagcttt tagcgcaatt aggggagtat ttacgtctgg
    50341 ggaataatcg tcaggcggtc acggattata tccgtcataa ctttggtcag acgccgctga
    50401 atcagctctc accggagcaa ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg
    50461 ttattccgca accacagcag cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca
    50521 atgcgctaaa acagctggtg accaaacttg cggcggcaac gggggaaccc agcaaacaga
    50581 tctggcaatc gatgctggaa ctttccgggg tgaacgatgg cgagttaatt ccagcgaaac
    50641 tgtttaacca tctggtgacc tggttgcagg cgcgccagac gttaagccag caaaatacgc
    50701 cgacgctgga atcactacag atggcgctaa aacaaccttt agatgccagt gaactggcag
    50761 cgttatcggc gtatatccag caaaaatatg gtctttctgc gcaatcatcg ctttcttctg
    50821 cccaggccga ggatattctt aatcagcttt atcaacggcg ggttaaaggg attgatccgc
    50881 gtgatatgca accgctgctt aatccttttc caccgatgat ggacacgttg caaaatatgg
    50941 caacgcgtcc cgcgctgtgg atactgttag tcgcgattat cctgatgctg gtctggctgg
    51001 ttcgttagcc ccgacgaaat gacagtatcg tgacaataat tcccagcacc gcagagatcg
    51061 ccccggcaag aaataccgaa gagtaaccaa acgtggtcgc cagcattccc gccagcggcc
    51121 cggagacgcc gagggcgata tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa
    51181 cttgtgaggg gacgcgttta accacctcca cgcccagcgc aggaaagata agcgaacatc
    51241 cggctccggt taacgccgcg cccgctaatg cgacccacgc acctggggct tgccagagca
    51301 gcaacaagcc caccgtttct acaagcagag agacaatcgc cactttcacg ccgccaaaac
    51361 ggtccggcat ccagccaaac atgacgcgca tcacgacaaa tgcgccgcca aactcggtaa
    51421 gagtaaagcc cgccatcgcc catcctttgc tggcaaagta gagcgaaacg aaagtcccga
    51481 taaccgcaaa accaacgcct tgtagtgcca gccctaaccc tggtttccag ataaacccga
    51541 caacgctcca cagcgatgga cgttctcccg ccagggccgg tactttgcgc actgtgccgt
    51601 tacaggccca cgccagtaag ggtaatacca ttgtggtgat cgccagtgcg gcaaaaccgt
    51661 aatggctatg aatcaacagg ccaagcggag caccaacagc gagggcaccg taaatcgcca
    51721 ttccattcca tgacatcact ttgccagagt gttttggccc tacgatgcct aacccccagg
    51781 tcagagcgcc tgtcagtaac tggctttcac caaacccaag aatcaaacgc ccgatgacca
    51841 acagggcaaa tttgaacggt gcggagacag gcaaaatcgc cgccagcagc aacgcgccgc
    51901 cagccagacc acaagctaac atcccctgaa gcgccgaacg ttttgcgcca tattgatcgg
    51961 ccagtcgccc ggcatagcca cgcgtcagca ccgtagccag aaactgaatc ccgacggcaa
    52021 tgccgaccat ggtattgcca tagcccagtt catgatgaac aaacagcggg ataaccggca
    52081 acggcagccc tacggtcatg taggtgagaa aaaccgcaaa agcgatgcgg aagagcgaaa
    52141 aattggcaga agatcgtgtt tcggtttggc ttacagcagt catgcattac tccagaatgc
    52201 agcgcaaggc gaggagtatc cccgtctcat ctctctggtt tcagggttac ggtgcgttgg
    52261 caggatttaa cgcgtacgtc ttttcagaag gaaatcgaca aagcgggaag tttgcctgga
    52321 actggtggcg attgtcaatg atgtgaaaaa gggaaccatc aggttccctt ttgcgttagt
    52381 gccggaggcc gcattggcgc gtaacgtcgg atgcgacgct ggcgcgtctt atccgaccta
    52441 ctccggccta ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg
    52501 ccgaagccga agctgttaga cataacagtg gtcagttcgc gatcggtcgt ttcggtcacg
    52561 atgttcagac ccgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag
    52621 ccgtgttcca gcatcagcag agagtagata gcttcctgca cacccgcagc gcccagtgag
    52681 tgaccggtca tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg
    52741 atagctgcca gctctttcac gtcgccaacc ggagtcgaag taccgtgaga gttcaggtaa
    52801 tcgattgggg tatcaacgcc atgcatcgcc atctgcatac aacgtactgc gccttcgcca
    52861 gacggagcaa ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag
    52921 atgtgagcac cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg
    52981 ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg
    53041 tcgttgtatt tagtagacag cgcacccatc gcgtcgaatt cgcaggccat ttcccagcac
    53101 agctcttcgc cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact
    53161 gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca
    53221 tgaattttaa acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc
    53281 acatacgggc caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaatacc
    53341 tggaaacgcg gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg gttattctgg
    53401 taagcttccg gagaaaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa
    53461 atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt
    53521 tttacgttgc cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg
    53581 atccctgaac gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg
    53641 gaaacaatgc ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac
    53701 atagagtaag tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc
    53761 catttaatag agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt
    53821 aagacgagta acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc
    53881 tgagggtaca cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct
    53941 ggaagagacg cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca
    54001 tccacatcct ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac
    54061 gctatggcag gcatttgatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt
    54121 acatttcatt agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca
    54181 acactggccg gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt
    54241 gcccggttgc catcgtttat tgctcaatga aggccgcgtg acgctggatt tatggtttgg
    54301 cgatattaac gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg
    54361 gtttctggac ggctttgcgc cagctaaaaa cccggatatg tggacgcaaa atctgtttaa
    54421 cgccatggca aggttggcgc gtccaggcgg cacgctggcg acatttacgt ctgccggttt
    54481 tgtccgccgc ggtttgcagg aagccggatt cacgatgcaa aaacgtaagg gctttgggcg
    54541 caaacgggaa atgctttgtg gggtgatgga acagacatta ccactcccct gctccacgcc
    54601 gtggtttaat cgtacgggca gcagcaaacg ggaagtagcg attatcggcg gtggtattgc
    54661 cagcgcgttg ttgtcgctgg cgttattacg gcgcggctgg caggtagcgc tttattgcgc
    54721 ggatgaagcc cccgcgctgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt
    54781 aagcaaacac gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg
    54841 gttttacgac caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg
    54901 ctgggatgag aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga
    54961 actggctgta gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg
    55021 cagcggcatt acttatccgc aaggtggttg gctgtgccca gcagaactga cccgtaatgt
    55081 gctggaactg gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt
    55141 atcccgtaag gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag
    55201 cgtagtggta ctggcgaacg ggcatcaaat cagccgtttc agccaaacgt cgactctccc
    55261 ggtgtattcg gttgccgggc aggtcagcca tattccgaca acgccggaac tggcagagct
    55321 gaagcaggtg ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca
    55381 ttgtattggt gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca
    55441 gcagcagaat cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt
    55501 tgatgtcagt gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc
    55561 aatggtaggc aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga
    55621 gcagaaagat gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt
    55681 aggttctcgc ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag
    55741 cgacgaaccg attccgatgg atgccagtac gctgacggcg ttaaacccga atcggttatg
    55801 ggtgcggaaa ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt
    55861 tgccggatgc ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac
    55921 gcgataagcg tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct
    55981 gctgaaacag gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac
    56041 cggcaccaat ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt
    56101 ggtcatcacc actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac
    56161 tggcaaacaa ctcatcatca ctggcatggt caaccatgcc gtcgattaat gccagaatgc
    56221 gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa aacgtggcac cggcttcagt
    56281 tgagcgcttc cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt
    56341 gtagtgcttt aatgaagcgt gccggacgct cgggaatgcc atttgtcaga taatccatca
    56401 cctgcgcatg tacccgccgt tgaaacacaa cacgatccgg ttcgaagtcg ccttccagat
    56461 tgtcgcaact gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgagtgcct
    56521 gcggcttcac ttcaacatct tcaaactggc tttgcgtttg ggcatcacgc ccatccgggc
    56581 aataccagta gccgaaatca accagctcac ggcgcgcttt tccggcaata caccagtgcg
    56641 aaatctcatg aattgcgctg gcataaaagc cgtgggcaaa gacaattcgg ttatacggta
    56701 cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa
    56761 attcatcggc aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt
    56821 tcattagttc atccccaacc agtggaggat ctcctgtccg tggctatcgt aaagtaattt
    56881 ggcactcatc accgccgaga cgataacaat catcgggcgg atcagctttt gtcctttgct
    56941 caacaccagc cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca tgacaaagcc
    57001 cgtcgcccaa atcactttgc cgccgagaat aaacagcagc agaccgccaa tgtttgacgt
    57061 tgcgttgagt aatttggcgt gagccgtggc tttggcgagg ttgaatccgc acagcgtaac
    57121 gaaggccagc gcgtaaaacg atccggcagc cgggccaaag aatccgtcat aaaaaccgac
    57181 gcagccaccg gcaattaacg cgaagggtaa accgtacatc cggcgctggc gatcttcttc
    57241 acccagcttt ggcatcagca agaaatagag gccgatacaa atcaccagaa tgggcaaaat
    57301 ctgccgtaag acatcagcct gaacgtactg taccaacagt gcgccgctca ttgagccgac
    57361 aaaggtcatg gcaatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa
    57421 gtagatagtg gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagcgcgtt
    57481 agcgggagac attcccgcag ccatcaatgc cggaatggtg agtaacccac cgccaccggc
    57541 aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg
    57601 ggaaaccata aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc
    57661 tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt
    57721 agaggtgcaa accagctttg cagttctgcc ccgcaaccat cgcctgatgg cggtaaaggt
    57781 tgatcttcac actccagact atcggcaggg caacgtaatc gtacatgcat atgcgcgcga
    57841 tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa
    57901 agttgttgtt taatcgccgg attaacaaaa atgcgcgtga cgtctttgtc ctgggcagcg
    57961 agtttgatca agctgaaaat ttctggcttc cacagcgtgg agacaacgtg tttaccgtcg
    58021 cgggaaacta agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc
    58081 ggcagttgca gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg
    58141 aaacgcccac cagcgggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg
    58201 ctcacctggc tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga
    58261 cgctgatcgg tacgcatgac ctgataatgt tcggactgta tcggcaacgt gtcagcgccg
    58321 acaatacagc cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt
    58381 attttttgcc acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca
    58441 atcgcggttt tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc
    58501 gcccgctgcc gtaacaggtg atccattaaa acgatcgcca gcatcgcttc agcgatcggc
    58561 actgcacgga tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg
    58621 ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata
    58681 tgagcaatga tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc
    58741 tggaaaccgt ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca
    58801 aaaccgtcgc caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg
    58861 tcggcatcca ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc actggcaaca
    58921 acggtgactt tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct
    58981 aacgcgtcga ttttgtccgg gtccgggcag aagaacggat tttgttcaac cagcgaccag
    59041 tctttgattt ccagcggaat gtcgcccatc tgggtcaagc agccgcggat ttcaatacca
    59101 aatttctcgg cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg
    59161 cgggcggaag aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg
    59221 taatcagcat ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg agagcgctgg
    59281 tcagtgtttt cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg
    59341 gagagaattt tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg
    59401 cgacgacggt cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca
    59461 ccatcgacga tgcagccgag cgccagccca tgcgattcgc cgaaggtggt tacgcgaaag
    59521 agttgtccaa ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac
    59581 ttaatcttta taaatcgcga aatattcacg ggccgccagc agctgctctt tggtgagcat
    59641 aaacacacca tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg
    59701 ttccataaga tgtaccatgc tgttgccgac ttcacaaatc aacacgccat catcagcaag
    59761 gtaatctgcc gcgtttccaa gaatgcgacg cgtcagttta aggccgtcgg tgccagatgc
    59821 caggcccagt tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc
    59881 gacatacggc gggttagtga caatcagatc gtactgcact ttcggcaaat cacggaacag
    59941 atcggaacga atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc
    60001 aaccgccagc gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata
    60061 agcacaggca atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg
    60121 cttgctaatg agtccggcaa atttattgtt gatcagttca ccaatcggcg agcgcggcac
    60181 cagcacgcgt tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta
    60241 agccaccgga atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc
    60301 gctggaggtc agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga
    60361 tggtaacacc agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat
    60421 atttgccgcg ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc
    60481 atttactgct tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata
    60541 atttcggcgg ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg
    60601 aggaaaaata cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt
    60661 agaaatagag acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg
    60721 tttcgccagt tgatggcggg gactcgcaag attaagcagg acacgattgt ccaccgaccg
    60781 cagcgtaaaa aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc
    60841 agccattatt tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat
    60901 gttcgcccgg atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg
    60961 gaattgtttt tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg
    61021 ttgattgccg cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg
    61081 aagcatattt tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca
    61141 tttcaccagg caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg
    61201 gaagagtggt tgccgccgga gttgccctga atgctggcag caaatgccgg agcagcgtaa
    61261 acgccttatc cggcctacaa atatgtgata attcaataaa ttgcaattca cttcgtaggc
    61321 ctgataagac gcatcagagc aatactacga actacagttc attcaataca tactgaaaac
    61381 gacacgtcga atcaatcggt tgcccactac tggtattcac attcaccaca atatcgtgta
    61441 gtactttatc cgttaccgga tgacgagttg gcgtaaccgt taccgttgat ttatcacaac
    61501 cgtaagaggt aaaggttaac aattctgata ccgcgcggca gtatgctcct ctgccatcat
    61561 tatcagaaaa attactgcca tcacagagtc tgccgccttc catcaggtag gtcgaaagcg
    61621 tggtggtcac tgttgacccc gtggactgaa ttcgtaacgc cttattatgg ctgacataat
    61681 tttcactcca ggagatatcg tatgaagtga ccggtatcgt tgggcatatt gtgttatgca
    61741 ctgtaggggc cgtatagctt ccaaatccgg gtaaatctgt ataggaataa aaggatgcga
    61801 tccaagggca accataatca tcagatgcaa cggtaatttt aaacgtcata tatgcacaac
    61861 gaaaaatttt cgtatctatg ccagggcaat ttgagttatt aattgtatca aagtcagtca
    61921 gtaccaaagt tggagctgaa cccgacccag accaggaaat aacccgcaac gtatcgcctt
    61981 tatacgcaaa agaatcatat ttcccatatg taacctcctt tggcgttatc accgtaacat
    62041 tgaaaacact gctcgctaca gcaccggtta ccttgtccat ggcataataa actctcacac
    62101 tgtcaaaact cgcactcata agtttatctc cagcataact cttagatgcg ccgacaacac
    62161 tcagaacgag cagcattagg ctcgcgaaaa ttatggtcca ttttttcatt tcagaaatac
    62221 tcaaaggttg ccgtcactgt ggcactaaag tctccggacg ttacatctcg cccgctcttt
    62281 gcctgtaacc aggtattaaa atttaggctg ttatttccct gattcagcgg gagcgtgacg
    62341 cctgttgtat tgttgattgg caccgatgtg cctgccgcag tttcaatacc aatcccagcg
    62401 ccggatgctg atgacgagga gtcaaaggcc agaaagccgg gtagagcact atcttctgtt
    62461 cctgtcaacg ttactttgac attaaacaat gtagaactat gacaatcttt caattgaaac
    62521 accaatggca ctcgctctga ctgcccaagg ttcattaaat cgctggcggc aatggcagga
    62581 aaatgtactt ctgccaactg gctaccatta atcactggcg tacacgattt actgattaaa
    62641 ttgccggtaa agtgcaaatt atctcctgct attgtggttc cactgcccag caacatcagg
    62701 ctactaataa aaaacaacgt acgctttttc ttcattggta atctacctgt aacgtggccc
    62761 aggcctcaaa gtccgcctct ggcagaacga caccactctt ttttactggc acagccgtta
    62821 acaccggtaa atcggtttca ttcaccataa gtggcgtatt gatcgtaaaa ctctgccctg
    62881 cctgtttgag ctgaatgcct aaaccagtga tattgctgct tactgcagca ttatcaaaat
    62941 cgcttagtgt gccactccag ctcaaggtca tttccattaa tgggtcgcga accgccgtat
    63001 cgcaggtaat ggaatacggc acggtttgct cataattgct gccattaatg tcatcaataa
    63061 gcacatcctg aaaagttatt tcaatggttt gagcgttgct aatcgtgcag ttaggcggct
    63121 gcactaaata accgtgaaaa gagacgtttg agagtgccat cgagcaaaag ccccacagca
    63181 aaataagcga gatccgtttc attggtaatc caccacaatt gtggcactgg cgttaaattc
    63241 agctgccgct aacgttgtac cactctgttt caccggcaca gcttccagaa cgggaactcc
    63301 cgaggaaaaa ttaaatggca accatgagcc agaggttaag tcgagtattg tgtgatcact
    63361 acttttctga acccgaatac caaacccagg aatacccgtg ctgataactg tttcaccgtt
    63421 aatcacgacc gttgtcgcct gcatctgtag acgcaggtca tccattgcca gattgttaca
    63481 aacaagcgag tagtcaatgg gacgtttata atcaacccca ttgatcttat taatgaatac
    63541 attaccaaac tcaactgccg caccatttac cgtacaagga ggaggggcat cgacaagcac
    63601 ccgtaaattc atacttactg acttactcgc tgcaactgca aagccgctac caacacaaca
    63661 tatcagtaac ccagttaccg cccagacaga acaagatcct ttcatactgg ctctcccggc
    63721 ctcagccttt gcggctctgc tcttcatcga cctggcaact gttaccgttg caagtgaaaa
    63781 agagcggcat ccgagcgcca tagtcgttga cataggtcag taccggcacg ctacccatct
    63841 tcacattcag cggtgcagag gttttcggcg acatcactat tggcgagaaa ccggctgcag
    63901 gattgccatt tttctgtgtg ctggcattac tgaaaatgac gtaatacggc gtcgggttat
    63961 tgacggtaaa ttcctgaccg ttgcgggtta aagtaacctt atattgccag gggtttttca
    64021 tgctgacgtt ctccagcgcc ttcggtcgcc agaacaactt aatacgcgtt tgtagtgcta
    64081 tttgcagcgt gttggctttg tttgattttg gcgggatctc acgcacgttg aagtagaaca
    64141 ggctttcacg atcggcaggc aatttgttga tatccggcat tccctgcact tttacctgcc
    64201 cgttcatcat tgaatcgatg cgttgcaccg gtggcagaac agttaacggt gaactgatct
    64261 tgttaccctt ttcatcttcg atccagcttt gggccagata aggcagtttc ggatcgttat
    64321 tgcgcaacgt cacgctgatt gatttgtcgc tttcattaaa tatcaaacgg gtacgatctg
    64381 gcgttacaga agcctgggcg acaaactagc agcgctaagc agtaacgcta gcgtcgtagc
    64441 acccaatttg gcagagcaaa gtaaatcaga catggatcat tccttaatca cgttattggt
    64501 tagctgaaac agaaacaggg ggattggtgg gtgtcacctg ttgcgtctgc tcctgaatga
    64561 caggctttat gtcatcagga acaaccggcg cgacgttgcc tttatgctgg catggcagta
    64621 acaagccgtt aaacagatcg gcaggcagcg ggtccggcag gttgatatcg caatgcgcaa
    64681 caccactcca gaacacactc atgtgttcgc caggtttcac cccagccaga taaacattgc
    64741 cgtcatcatc gacaaggccc actgtctgct cgttatcatt ttttacttct gcgccaaacg
    64801 gaggatggct gccgtcttgt aaacgcagca ccgccatcgc tttttgacca ctgatgacgg
    64861 caaatttgcg gtagccaatc gccccttcag ttagcgtggc ttgtaccacc gactgggttg
    64921 cttcggcgtt ttccggcagt ttgttgaggt cgatatacgc ctgattgcgg taatagttat
    64981 tgacgtcaga aacgacggct ttaccaaaca tattggtata aacagcagca ccgttacctt
    65041 ctaccggaac atcggcaacg ccatcggcat caatcaacaa gcgtgtcccg cccatattct
    65101 gggtacggtg aagtgcgcca ccgtgggcag taagcgttgc gccgccctgt aacgacaagc
    65161 ccgcagaggt gtattgccct tcatggtagt tcgcactgag gtcaacctgc gccagcgaac
    65221 catcatggct gtaatagccg tcaacgctgg tgtgtttgtc actggtgccg acgttcaact
    65281 gatagtgagt cgcgtcatcg acacggctga aataaccgac ctgactgctg tccgtcccac
    65341 tgccatagtt accgttatag ctaacggtac tgttgtcacc ccacggcata ctgagcgaaa
    65401 tgtacatgcc tttgtcggcc tggttgtcat actcgtaacg gtagccagtc atcgagatgc
    65461 tgacattacg aatactgccc atattgaaat agtgcgagag catgatgttg tagtttgtct
    65521 gctcctcgcg atcccagtag gtatggcggg tatagttgag ataaaccgaa acacccgcat
    65581 cgcggaagtt ctggttataa gtggcagtgt acatctcttt gtcgttgccc gtgcgtacca
    65641 ttccgctgtc actggcatcc agatactcgc tcatggtcat aaagttctct tccgagaagc
    65701 gatatccagc aaaagtaacg cggctgttga gctggtcaaa gtctttggaa taactcacac
    65761 ggaaggagtt accgtccagc gaacctttgc cataagcggt gtctttatcc agtttggtgt
    65821 gcgagtgagt aacatcaaac gcgaccgcgc cgaatgtaga caaatcgcga ccgacgccaa
    65881 gcgccgcaga ctgatagttt tcatctccca gtgcgccgcc atataacgac cagccgttag
    65941 cgatccccca ggaagcttca gcaccagaaa aaaattcacc ctcgacatgg tgtccccact
    66001 cttgcggacg gcccatcatg atcttatagc gaacctgacc tggacgagtg aggtatggca
    66061 tcgaggcggt gctgatgtca tattcctgca cctggccgtt ctgttcttca atgcgaatat
    66121 gcaacgtacc ggagacagaa tcaccaagat cctgaatacg aaacggtcca gccggaacct
    66181 gcgtttcgta aatcacacgc cccatctggc tgacggtcac ttttgctgtg gtgtgtgcca
    66241 cgccggaaat gtctggcgcg tagccgcgca gattgggagg caacatttga tcgtcagtac
    66301 tgacactgcc accaacatag ttaaaaccat caaaaatatc ggatctgagg taatcctcgc
    66361 ccagcgccag tttggctttt aatgatggta acgcccgcta ggcatagtag cgactccact
    66421 cccatttttt ttgagtttca tcgccgctga attcatcgtc atcattactg cgagtatgtt
    66481 gatagttagt ctgccagtca gcacgcatac gccacggccc caggttaacc ccgaccgtcc
    66541 cgttgccgct gatctcgtta ctatcatcac cgccattttc ttcgtgccgg gtttgtgcgt
    66601 tgatgctgta gtccgcgacg atcccggaga tgccgtcatc ccaacgtgaa ggcggatccc
    66661 agtcgggata agtatattcg aggtaagcct gtggcagtga aatgactaat gcggactggc
    66721 ttaaatcaac cttaatttcc atgccttcca gttgaccgga tttcaggcat ttagcatcgt
    66781 ggctccattg cagatttttc gccacgtctt cttttaaacc aaactgcgct accagttccg
    66841 gtgtcagaca agcatagctt ttgctcgcgt catcttcacc agcataccag taaatatcgt
    66901 actcttccgc caatggctgt ttatttagtt gaacctgtaa attgtatttt ccgggctcaa
    66961 catatccctg gctggaaaaa cgcttcagat caatttttgt gtcgcctttt aattccagaa
    67021 aacgggaatc aaactgaatg tcgtcttcag cccagacact gctataactc cctgacattg
    67081 ccagcgcaat gcaccacgga agaatccgta atcgaaaaag agaatggtca ggcatacacg
    67141 tttctatccg tttttattct tgttcggtca aaatccattt aaatccccca tagcagtgtc
    67201 gctatggggg tatgccttac aggtaagtaa tctggaaggt ggtatttgct tcgaaattac
    67261 ccagatctgg cgcatcagct gcgccgacca gccaggcttt aaagttcagt gtctgcttcg
    67321 ctttgccttt attggtagaa gtgtctttca ctattttctg gtcaataccc atgccgcttt
    67381 tgtaagaggt acccagagag tcaccgatcg ccaggctaac attgttaaat gccgcacagg
    67441 tatcggtatt gaaaatggtg taataattgc cgctatttgc agaagaaacg gtaccagtaa
    67501 aggtagtggt cattgtttcc tgagtatcaa atacgcaatc ttgcagacga atctggaaac
    67561 ttttcggcgt ggtggtgccg ttatttttca gtttttcggc accgatatca cccatatcaa
    67621 cttccagttt gtgatcgtcc ggaacgatag aacacactga gtcttcaatg gtgccgtaaa
    67681 aacgtgcagt accgttgtta cccgcagcga ttgttgccgt agaggtgaac acgcccatca
    67741 ccattgccgc agcaatagct gttttaacaa acttactcat tatttgtcct taaactataa
    67801 ataattaaaa atataaacat tgcaatacat tgattcagtc aataaccaat gtttataccc
    67861 agccattgaa tacggcatgc gatccaaaaa gaacaggtcg tagaaaattg tattcgttgg
    67921 tgtggggccg catagtgatg tttccacacc cctgatgcaa tttaatttta ctaaagagta
    67981 aatgtagatt taattaatat attgacagaa gaaaggttat cttaaggaca tccttaaaaa
    68041 atacggaata agtatttaga aaaaatagaa agtatatcaa taatgaatat caacagctta
    68101 aaaaacaaac aatgaacata taatgcgatt ggcatttatc cacttttgac aatataatat
    68161 ataaacacta tagccagaat ttaatcaagt ttcaattaca tcctgcctga ttattcaact
    68221 ctattttctc tattatctgt attaataaat gacaatgagt gtcaaacaga cattattcat
    68281 ttcaacatgt aactttgggc aaaatcaaga tacgcgcaga agaataattt ccgaatgaaa
    68341 gtagaaagtg ttttatttat agatggggag agtcttcgat agtagtgaga ttgacagata
    68401 tccgctgcct gatgagcaat tatgcccaat gaatctacct cattgggcat aatttggaac
    68461 tcatcagata gcttttgcca tcttcagatt acacgggctc atctgccagt tgaatgtgcc
    68521 gtttccgctt tcatcaagcg ttacgctggc aatggccgag gtggtaaaca taggcggcgt
    68581 ttcgcccgga cataactcgg caaccagata cccgactaac ggtaagtgag agatcaccag
    68641 taccgaagca acaccttcat tggtcaacgc ctgcaaatag gcactgacca gaccaacatc
    68701 gccgcagggc gttaactccg gcagaacttc tgcactggaa ggcaggttca gacaatcccc
    68761 tacttcttcc agtgtttgct cggctcgcag gaacgggctc accagaacgc gttcgatttc
    68821 cactttttga cctttcagcc agttcgccat caggcgagat tcgtcacaac cattagtggt
    68881 cagaggacga accgaatcac tggcggcatc aagggctgcg tcgccgtgac gcatgataaa
    68941 aacttgcata ttgcaccgct tttgttaacc agattcgtcc gttttctttc atctgaaacc
    69001 agagaaagaa aacggtggcc ggcattgtgc cttatccatt caccgaatga aacgctgttt
    69061 tttacctcaa tggcgtaagt atagtcaata tgcgtttaca ttttgaccaa cactccgcca
    69121 ttcagcgcgg attcatatag ctttgacctt cttattgaag gtcagttgca gttgttttcc
    69181 aaaaactttc cccacgcgcg cccatctcga ccaaacgctc gcaaggggta aaacgggaac
    69241 catactgcgt ggccagtcgt tgcattattg caaccacttc gcccgcgccg agagaatcga
    69301 tatagcggaa cggtccaccg agaaatggcg gaaaaccaat gccaaatacc gcgccaatat
    69361 ccccgtcacg cacgctacgg ataacctgct catcaacaca acgtactgct tcattcagca
    69421 tcaacatcac acaccgttca gcaacctgcg gtgcggagag tcgcccctgc ccttgtgcgc
    69481 caatgagcgg gtaaatggcg ggatcgacct gttttttgct tttacgccct ttctgaccat
    69541 aaagatagaa accccgacca ttttttctgc ctttgcgatc gtcgttcaaa attgaagaaa
    69601 caacatttgc aggcgcgcta aaacgttctc cataagcggc ttccagtaca ggaataattt
    69661 tagtcccggt gtcgattcct acctcatcca aaagttggat tgggcctacc ggaaaaccaa
    69721 atttcactag cgcggcatca atgtgctcta cccgttcacc ttcggtcagc atgcggatag
    69781 cttcattaat gtaaggcgct aagatgcgat tgacgtaaaa cccggctttg tcacgcacga
    69841 caatgggcgt tttgccctgt tttttcgcca gttttaccgt agtggcgatg gtttgcgccg
    69901 atgtacccgc atgaggaata atctctacca gcggcatttt ttccacagga ctgaagaaat
    69961 gcaggccaat aacttgctca ggtcgcgcgg cgtgagcggc gatatcacca atcggtaaag
    70021 atgacgtgtt cgaagcaaaa atggtatgag cggcgcaatt ttgctccact tccgccacca
    70081 tctgttgttt taattcgaga ttttcaaaca ccgcttcaat aatcagatcg cgatgggcaa
    70141 agccgcgata gtccgtcgtt ccggagatta atgccagctg tttgtcacgt tcgctggctt
    70201 tgagatgacg acggcgaact ttgccctcca gctgatccca actgtacttc agcgcatgat
    70261 ttatgccctg cgggttgata tctttaattc tgaccggaag ccccgcttta caagcagtga
    70321 cataagcaat accgccgccc atcaagccac cacctaaaat tcccacgctg tttaatggcg
    70381 caggcggtgc atcactaccg ggatctttct tcacgtccgt actggcaaaa aagatactac
    70441 gcaaagcttg cgattgcggc gtcatcgcca gttcgccaaa ggcgcgagct tcagcgtcat
    70501 agccgctgct ggtgccctgc gctaatcccg tttcaacaac ctccaggatg cgttctgtcg
    70561 ccggataatt gccttgagtt ttgtgttctg ttttcttgcc gaccattttg aacagcagcg
    70621 cacgacctaa cggccccgcc agaatacgct cgcgtacagg tagagggcgg gaagatgggc
    70681 gatccttctt cgccagctca acagcggctt ccagcaaaat ggagtgcgga acaacgtcat
    70741 ccaccagccc cagctttaat gcctgtttcg cccgaagttg ttttccggtg agaatcatct
    70801 ctaatgctgt actgacgcct atcagacgcg gtaaacgctg ggtgccgcct gaaccgggta
    70861 acaatccaag ttgtacttca ggcaaaccga gcaccgtttt aggatcgtca gtacaaacgc
    70921 gaccgtggca cgccagcgcc agctccagcc caccgcccag gcaagcgcca tgaatagccg
    70981 cgataaccgg aatgggcaaa gcatgaatct ccgccatcaa ctgttggccc tgccgcgcca
    71041 gtgcttccgc ttcttgcgcc gttttgcagt tgccaatcat gttgatgtct gctccggcga
    71101 taaaattgtc cggtttcgcg gagataaaca ccacgccgcg caactctttg ttttcacgga
    71161 gttgcttaat aatggcgcgc acctgcgagg caaattccgc cttcagggta ttcattttct
    71221 catccggtac atcgatggtg atgatagcaa tgttgtccag acgaacatta agagtaaacg
    71281 ctgatgccat ttccattatt ccgcctccag aaccattgcc gcgccaagcc caccggcagc
    71341 acaggcggta actaaaccaa atccaccgcc gcgacggcga agttcatgca atgtctgggt
    71401 aatcatccgc gcgccggtcg ccgcgaaggg atgcccgtaa gcaatcgaac cgccaagcac
    71461 gttaaatttg ctatcgtcca cttcgccagt ggcatgtgca cgccccagta cttcacgagc
    71521 aaaacgttca ctacccagca actgaatatt cgccagtgtc tgagctgcaa aggcttcgtg
    71581 catatcgatc aatgtcagtt cgctcatcgt caaaccggca cgttccagcg ccagcggtgt
    71641 tgaccaggct ggaccgagca acatgtcctg ccagacatca atcgcagtaa atgcgtagct
    71701 gcgcagatac cccagcggca ccagccctaa ttctttcgcc cgggattcag tcatcaggat
    71761 caccgctgcc gcgccatcgg tcagcggcgt actgtttgcc gccgttaccg ttccgtgttt
    71821 gcgatcaaac gccgggcgca gctttgcgta atcggcaagc gaggaattac cgcgaatatt
    71881 gttgtcttcg acaagcggtt gtttataagg agggataaag gcagtcatca cctcttcttt
    71941 gagttttccg tctgaccatg cctgagcggc acgctgatgc gaacggtgcg ctaatgcatc
    72001 ttgctgttct cgggtgatgc cgtaggtttt cgccatttgt tcggcggtgt cgcccatccg
    72061 caagccagta gaatattctg ctaccgcagg cggtacgggc attaagtcgc gcaaacgcag
    72121 gcgagagaag agtttcaggc gctggctcat ggttcgagct ttgttgacat caaccagcac
    72181 gcgcgccagt tttttactga cgccaattgg caatacggaa gaggaatctg ccccaccggc
    72241 aatccccgct cggatagttc ccgccatcag gctttctgcg acgtttgcaa ccgcctggaa
    72301 actggtagcg caagcgcggc tgacgctgta agcatcggta tgcacattca ttccggtacc
    72361 gagaacaatt tcacgcgcaa tgttgggggc ttcaggcatt tgtacgacct gaccaaagac
    72421 cagctgttca atcacttcag cggggatctc gctgcgtgcc agcagttcgc ctaccaccat
    72481 cttccctaaa tcaaccgcgg gaatgccatg aaaagccgtc gcctggcggg caaaaggcgt
    72541 acgtaaaccg ctaacaatgg cgatacgatc gccctggcgg gtaaccagcg gtaaaacctg
    72601 acccataaca ctcccctgta aaaaaataaa taaagtggtc tgacctgatc atagtcttaa
    72661 ctattttttt acatttagcc aagcggagaa aaaggaaagt gggagctatg acacagtgaa
    72721 gacaggagaa agaaaagaaa acgcccctgc cagcaactga caggggcgaa cgtacccgag
    72781 aggaattaac gcagacccag ctggaagatc agcatttcag cttcgcaggc aaaagtgaaa
    72841 tcgatatcca ggcgcacacc gtcagactct tcagtgaaag tcggggagat tttgcacggt
    72901 tcggattcca cgctacgggc tttttcagtc agcgccgcca gcgtttgttc tgcttctgcg
    72961 cggtttgcaa acacgcggct gtaagacgcg gtgcagtcgg agttgtccat aatggtgcca
    73021 acatccatac agcagcaaac cggggtttca tcagcactac atttactcat cgttgatttc
    73081 ctctgtatgt gcacccaagg tgccagataa acgttgtgga tattttacgc ttccggaaag
    73141 tgctgctcca gttgttaatt ctgcaaaatc ggataagtga ccgaaatcac acttaaaaat
    73201 gatctaaaac aaaattcacc cgaatccgcg agtgcgcaac ctccaaattt tgccagctgg
    73261 atcgcgtttc ttagatcata tttgaaaaaa gatggaaaca tacttgcagc attccagctg
    73321 gtccgaccta tactctcgcc actggtctga tttctaagat gtacctcaga ccctacactt
    73381 cgcgctcctg ttacagcacg taacatagtt tgtataaaaa taaatcattg aggttatggt
    73441 catgagccag aaaaccctgt ttacaaagtc tgctctcgca gtcgcagtgg cacttatctc
    73501 cacccaggcc tggtcggcag gctttcagtt aaacgaattt tcttcctctg gcctgggccg
    73561 ggcttattca ggggaaggcg caattgccga tgatgcaggt aacgttagcc gtaaccccgc
    73621 attgattacc atgtttgacc gcccgacatt ttctgcgggt gcggtttata ttgacccgga
    73681 tgtgaatatc agcggaacgt ctccgtctgg tcgtagcctg aaagccgata acatcgcgcc
    73741 tacggcatgg gttccgaaca tgcactttgt tgcaccgatt aacgaccaat ttggttgggg
    73801 cgcttctatt acctctaact atggtctggc aacagagttt aatgatactt atgcaggcgg
    73861 ctctgtcggg ggtacaactg accttgaaac catgaacctg aacttaagcg gtgcgtatcg
    73921 cttaaataat gcatggagct ttggtcttgg tttcaacgcc gtctacgctc gcgcgaaaat
    73981 tgaacgtttc gcaggcgatc tggggcagtt ggttgctggt cagattatgc aatctcctgc
    74041 cgggcaaact cctcaagggc aagcattggc agctaccgcc aacggtatcg acagtaatac
    74101 caaaatcgct catctgaacg gtaaccagtg gggctttggc tggaacgccg gaatcctgta
    74161 tgaactggat aaaaataacc gctatgcact gacctaccgt tctgaagtga aaattgactt
    74221 caaaggtaac tacagcagcg atcttaatcg tgcgtttaat aactacggtt tgccaattcc
    74281 taccgcgaca ggtggcgcaa cgcaatcggg ttatctgacg ctgaacctgc ctgaaatgtg
    74341 ggaagtgtca ggttataacc gtgttgatcc acagtgggcg attcactata gcctggctta
    74401 catcagctgg agtcagttcc agcagctgaa agcgacctca accagtggcg acacgctgtt
    74461 ccagaaacat gaaggcttta aagatgctta ccgcatcgcg ttgggtacca cttattacta
    74521 cgatgataac tggaccttcc gtaccggtat cgcctttgat gacagcccag ttccggctca
    74581 gaatcgttct atctccattc cggaccagga ccgtttctgg ctgagtgcag gtacgactta
    74641 cgcatttaat aaagatgctt cagtcgacgt tggtgtttct tatatgcacg gtcagagcgt
    74701 gaaaattaac gaaggcccat accagttcga gtctgaaggt aaagcctggc tgttcggtac
    74761 taactttaac tacgcgttct gataacgcgt tcgcctggat aaagtcacct gcatagcagg
    74821 tgactttaac tcccccactt caccgaaagt agtgtcctca ttgcttacct cctgagtttt
    74881 gcaaacagcc tgttggcagc ttgccctttc aaatcaataa gcggtatcca cagacaaacc
    74941 tgaagggaaa ggcatatttt caggcgttct gctcgtcctt ctcaaagagt ttacttttct
    75001 gcatttccag gatac
//
LOCUS       DAQSWQ010000005        74516 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.005, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000005 DAQSWQ010000000
VERSION     DAQSWQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.005"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..942
                     /locus_tag="R8G18_000329"
     CDS             <1..942
                     /locus_tag="R8G18_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEE9794133.1"
                     /translation="PMLGEKRLVSSVTKEDLLFVRRDLLTGYQKLSNGKISSIKGRSV
                     VTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            978..1664
                     /gene="mlrA"
                     /locus_tag="R8G18_000330"
     CDS             978..1664
                     /gene="mlrA"
                     /locus_tag="R8G18_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HEE9794134.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1724..1831
                     /locus_tag="R8G18_000331"
     CDS             1724..1831
                     /locus_tag="R8G18_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HEE9794135.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1812..2543)
                     /gene="yehW"
                     /locus_tag="R8G18_000332"
     CDS             complement(1812..2543)
                     /gene="yehW"
                     /locus_tag="R8G18_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HEE9794136.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2548..3474)
                     /gene="yehX"
                     /locus_tag="R8G18_000333"
     CDS             complement(2548..3474)
                     /gene="yehX"
                     /locus_tag="R8G18_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HEE9794137.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3467..4624)
                     /gene="yehY"
                     /locus_tag="R8G18_000334"
     CDS             complement(3467..4624)
                     /gene="yehY"
                     /locus_tag="R8G18_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HEE9794138.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4631..5548)
                     /gene="osmF"
                     /locus_tag="R8G18_000335"
     CDS             complement(4631..5548)
                     /gene="osmF"
                     /locus_tag="R8G18_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HEE9794139.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5800..8097)
                     /gene="bglX"
                     /locus_tag="R8G18_000336"
     CDS             complement(5800..8097)
                     /gene="bglX"
                     /locus_tag="R8G18_000336"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HEE9794140.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8293..10008
                     /gene="dld"
                     /locus_tag="R8G18_000337"
     CDS             8293..10008
                     /gene="dld"
                     /locus_tag="R8G18_000337"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEE9794141.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10047..10979)
                     /gene="pbpG"
                     /locus_tag="R8G18_000338"
     CDS             complement(10047..10979)
                     /gene="pbpG"
                     /locus_tag="R8G18_000338"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEE9794142.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11153..11740)
                     /locus_tag="R8G18_000339"
     CDS             complement(11153..11740)
                     /locus_tag="R8G18_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEE9794143.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11910..12488
                     /locus_tag="R8G18_000340"
     CDS             11910..12488
                     /locus_tag="R8G18_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEE9794144.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12618..13379)
                     /locus_tag="R8G18_000341"
     CDS             complement(12618..13379)
                     /locus_tag="R8G18_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEE9794145.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13432..14868)
                     /gene="mdtQ"
                     /locus_tag="R8G18_000342"
     CDS             complement(13432..14868)
                     /gene="mdtQ"
                     /locus_tag="R8G18_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HEE9794146.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15092..15175
                     /gene="yohP"
                     /locus_tag="R8G18_000343"
     CDS             15092..15175
                     /gene="yohP"
                     /locus_tag="R8G18_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HEE9794147.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15548..16495)
                     /gene="dusC"
                     /locus_tag="R8G18_000344"
     CDS             complement(15548..16495)
                     /gene="dusC"
                     /locus_tag="R8G18_000344"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HEE9794148.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16734..17132
                     /locus_tag="R8G18_000345"
     CDS             16734..17132
                     /locus_tag="R8G18_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEE9794149.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17129..17824
                     /locus_tag="R8G18_000346"
     CDS             17129..17824
                     /locus_tag="R8G18_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HEE9794150.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            17954..18838
                     /gene="cdd"
                     /locus_tag="R8G18_000347"
     CDS             17954..18838
                     /gene="cdd"
                     /locus_tag="R8G18_000347"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEE9794151.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            18988..19707
                     /gene="sanA"
                     /locus_tag="R8G18_000348"
     CDS             18988..19707
                     /gene="sanA"
                     /locus_tag="R8G18_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HEE9794152.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19710..19949
                     /locus_tag="R8G18_000349"
     CDS             19710..19949
                     /locus_tag="R8G18_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HEE9794153.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20143..21381
                     /gene="preT"
                     /locus_tag="R8G18_000350"
                     /pseudo
     CDS             20143..21381
                     /gene="preT"
                     /locus_tag="R8G18_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21375..22610
                     /gene="preA"
                     /locus_tag="R8G18_000351"
     CDS             21375..22610
                     /gene="preA"
                     /locus_tag="R8G18_000351"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEE9794154.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22737..23747)
                     /gene="mglC"
                     /locus_tag="R8G18_000352"
     CDS             complement(22737..23747)
                     /gene="mglC"
                     /locus_tag="R8G18_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HEE9794155.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23763..25283)
                     /gene="mglA"
                     /locus_tag="R8G18_000353"
     CDS             complement(23763..25283)
                     /gene="mglA"
                     /locus_tag="R8G18_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HEE9794156.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25344..26342)
                     /gene="mglB"
                     /locus_tag="R8G18_000354"
     CDS             complement(25344..26342)
                     /gene="mglB"
                     /locus_tag="R8G18_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HEE9794157.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26622..27662)
                     /gene="galS"
                     /locus_tag="R8G18_000355"
                     /pseudo
     CDS             complement(26622..27662)
                     /gene="galS"
                     /locus_tag="R8G18_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27804..28961)
                     /locus_tag="R8G18_000356"
     CDS             complement(27804..28961)
                     /locus_tag="R8G18_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HEE9794158.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(28978..29646)
                     /gene="folE"
                     /locus_tag="R8G18_000357"
     CDS             complement(28978..29646)
                     /gene="folE"
                     /locus_tag="R8G18_000357"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEE9794159.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29904..30740
                     /gene="yeiG"
                     /locus_tag="R8G18_000358"
     CDS             29904..30740
                     /gene="yeiG"
                     /locus_tag="R8G18_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEE9794160.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30772..32763)
                     /gene="cirA"
                     /locus_tag="R8G18_000359"
     CDS             complement(30772..32763)
                     /gene="cirA"
                     /locus_tag="R8G18_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HEE9794161.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33055..34524)
                     /gene="lysP"
                     /locus_tag="R8G18_000360"
     CDS             complement(33055..34524)
                     /gene="lysP"
                     /locus_tag="R8G18_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HEE9794162.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34729..35610)
                     /locus_tag="R8G18_000361"
     CDS             complement(34729..35610)
                     /locus_tag="R8G18_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9794163.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35709..36758
                     /locus_tag="R8G18_000362"
     CDS             35709..36758
                     /locus_tag="R8G18_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEE9794164.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36832..37689
                     /gene="nfo"
                     /locus_tag="R8G18_000363"
     CDS             36832..37689
                     /gene="nfo"
                     /locus_tag="R8G18_000363"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEE9794165.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37693..38781
                     /locus_tag="R8G18_000364"
     CDS             37693..38781
                     /locus_tag="R8G18_000364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEE9794166.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38837..40087)
                     /locus_tag="R8G18_000365"
     CDS             complement(38837..40087)
                     /locus_tag="R8G18_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEE9794167.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40187..41128)
                     /gene="rihB"
                     /locus_tag="R8G18_000366"
     CDS             complement(40187..41128)
                     /gene="rihB"
                     /locus_tag="R8G18_000366"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HEE9794168.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41258..41956
                     /gene="yeiL"
                     /locus_tag="R8G18_000367"
     CDS             41258..41956
                     /gene="yeiL"
                     /locus_tag="R8G18_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HEE9794169.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42023..43273)
                     /locus_tag="R8G18_000368"
     CDS             complement(42023..43273)
                     /locus_tag="R8G18_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEE9794170.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43367..44305)
                     /gene="psuG"
                     /locus_tag="R8G18_000369"
     CDS             complement(43367..44305)
                     /gene="psuG"
                     /locus_tag="R8G18_000369"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEE9794171.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44293..45234)
                     /locus_tag="R8G18_000370"
     CDS             complement(44293..45234)
                     /locus_tag="R8G18_000370"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HEE9794172.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45692..47383)
                     /gene="fruA"
                     /locus_tag="R8G18_000371"
     CDS             complement(45692..47383)
                     /gene="fruA"
                     /locus_tag="R8G18_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HEE9794173.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47400..48338)
                     /gene="fruK"
                     /locus_tag="R8G18_000372"
     CDS             complement(47400..48338)
                     /gene="fruK"
                     /locus_tag="R8G18_000372"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEE9794174.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48338..49468)
                     /gene="fruB"
                     /locus_tag="R8G18_000373"
     CDS             complement(48338..49468)
                     /gene="fruB"
                     /locus_tag="R8G18_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HEE9794175.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49836..51017
                     /gene="setB"
                     /locus_tag="R8G18_000374"
     CDS             49836..51017
                     /gene="setB"
                     /locus_tag="R8G18_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HEE9794176.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51014..51268)
                     /locus_tag="R8G18_000375"
     CDS             complement(51014..51268)
                     /locus_tag="R8G18_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEE9794177.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51423..51995
                     /gene="yeiP"
                     /locus_tag="R8G18_000376"
     CDS             51423..51995
                     /gene="yeiP"
                     /locus_tag="R8G18_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HEE9794178.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52218..53684
                     /locus_tag="R8G18_000377"
     CDS             52218..53684
                     /locus_tag="R8G18_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEE9794179.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53802..54788
                     /gene="yeiR"
                     /locus_tag="R8G18_000378"
     CDS             53802..54788
                     /gene="yeiR"
                     /locus_tag="R8G18_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HEE9794180.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54827..55540
                     /gene="lpxT"
                     /locus_tag="R8G18_000379"
     CDS             54827..55540
                     /gene="lpxT"
                     /locus_tag="R8G18_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HEE9794181.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            55952..56518
                     /gene="mepS"
                     /locus_tag="R8G18_000380"
     CDS             55952..56518
                     /gene="mepS"
                     /locus_tag="R8G18_000380"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HEE9794182.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56699..58255
                     /locus_tag="R8G18_000381"
     CDS             56699..58255
                     /locus_tag="R8G18_000381"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HEE9794183.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58337..60151
                     /locus_tag="R8G18_000382"
     CDS             58337..60151
                     /locus_tag="R8G18_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEE9794184.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60152..61246
                     /locus_tag="R8G18_000383"
     CDS             60152..61246
                     /locus_tag="R8G18_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HEE9794185.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61246..62271
                     /locus_tag="R8G18_000384"
     CDS             61246..62271
                     /locus_tag="R8G18_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9794186.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62273..63862
                     /gene="yejF"
                     /locus_tag="R8G18_000385"
     CDS             62273..63862
                     /gene="yejF"
                     /locus_tag="R8G18_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HEE9794187.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63866..64210)
                     /locus_tag="R8G18_000386"
     CDS             complement(63866..64210)
                     /locus_tag="R8G18_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794188.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64543..65663)
                     /locus_tag="R8G18_000387"
                     /pseudo
     CDS             complement(64543..65663)
                     /locus_tag="R8G18_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65691..66386)
                     /gene="rsuA"
                     /locus_tag="R8G18_000388"
     CDS             complement(65691..66386)
                     /gene="rsuA"
                     /locus_tag="R8G18_000388"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HEE9794189.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66536..68296
                     /locus_tag="R8G18_000389"
     CDS             66536..68296
                     /locus_tag="R8G18_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEE9794190.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68421..68705
                     /gene="rplY"
                     /locus_tag="R8G18_000390"
     CDS             68421..68705
                     /gene="rplY"
                     /locus_tag="R8G18_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEE9794191.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68844..69851)
                     /gene="yejK"
                     /locus_tag="R8G18_000391"
     CDS             complement(68844..69851)
                     /gene="yejK"
                     /locus_tag="R8G18_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HEE9794192.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70033..70260
                     /locus_tag="R8G18_000392"
     CDS             70033..70260
                     /locus_tag="R8G18_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HEE9794193.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70280..72040
                     /gene="pbgA"
                     /locus_tag="R8G18_000393"
     CDS             70280..72040
                     /gene="pbgA"
                     /locus_tag="R8G18_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HEE9794194.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72115..72191
                     /locus_tag="R8G18_000394"
     tRNA            72115..72191
                     /locus_tag="R8G18_000394"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72149..72151,aa:Pro,seq:ggg)
     gene            complement(72294..74516)
                     /gene="yejO"
                     /locus_tag="R8G18_000395"
                     /pseudo
     CDS             complement(72294..74516)
                     /gene="yejO"
                     /locus_tag="R8G18_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18288 a        19647 c        18288 g        18293 t
ORIGIN      
        1 ccaatgttag gtgaaaaaag actggtttca tctgtaacaa aagaggattt gcttttcgta
       61 aggagagatc tgttgaccgg ttatcaaaag ctttctaacg gaaaaatttc ttccataaaa
      121 ggacgctcag tggtcacagt aaactactat atgacaacca tagctggaat gtttcaattt
      181 gcaacagata atggttatac atcaggaaat ccatttaacg gtctgacacc attaaaaaag
      241 tccaagatag aaccagatcc cctcacccgt gacgaattta ttcgttttat tgaggcttgc
      301 cgtcatcaac aaacaaaaaa cctgtggatt atcgctgtat acacgggtat tcgtcacggg
      361 gagctggtat cactggcctg ggaagatata gaccttaaag cgaggactat aaccatccgc
      421 cggaattata caaaactagg cgaattcact ccaccaaaaa ctgatgctgg taccggaaga
      481 acgattcatc tagttcaacc agctattgat gctcttaaaa gccaagcgga aatgaccatg
      541 cttggaaaac agcattctgt agaggtaaag cagagggaat atgggagaag tactgtgcat
      601 aaatgcactt ttgtttttag tcctcaggta ataaaacagc ggcagttttc cggaccgcac
      661 tataaggttg actccatcag ggagtcatgg acaagtatct taaaacgcgc aggtctgaga
      721 cacagaaaat cgtaccaatc caggcatact tatgcatgct ggtcacttgc cgctggagct
      781 aatcctagtt ttatcgcaag ccagatgggc cacacaaacg cacaaatggt attcaatgtt
      841 tacggagcat ggatgaaaga caacaatcac gaacagatag aactccttaa caaaagacta
      901 tctgaaagtg tcccatgtat gccccataag aaagtggggt aaaataaaaa cttgtaaaat
      961 cagttagttt acccttaatc cctgtcacgt tacgcgcgtg gcagaggcgt tacgggttgc
     1021 tgaaaccgca acggacagac ggcggtcatc gactgttcaa cgatgccgat attgaccgga
     1081 tccgcgagat caaacgctgg atcgacaacg gcgtgcaggt cagcaaagtt aaaatgctgc
     1141 tcagtaatga aaatgttgat gtgcagaacg gctggcgcga tcagcaagaa acattactga
     1201 cctacctgca aagcggcaat ctgcatagcc tgcgaacgtg gatcaaagag cgcggtcagg
     1261 attaccccgc ccagacactc accacacatc tgtttattcc tctgcgccga cggcttcagt
     1321 gccaacaacc gactctccag gcgctgctgg cgatcctcga cggcgtactg atcaactaca
     1381 tcgccatttg tctggcttcg gcacgtaaaa aacagggtaa agatgcgctg gtggttggct
     1441 ggaatattca ggataccacc cgtctgtggc tggagggctg gattgccagt caacaaggat
     1501 ggcgcattga tgtcctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc
     1561 gtacattgct ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca
     1621 gttggcaaga acaaggccat gatattttcc cactcggcat ttaatgattc gttaacaaat
     1681 gcgctttact gtacaatcct ttcgttaaca taaggagtgc aatatgcgca tagctaaaat
     1741 tggggtcatc gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc
     1801 aggttatacc tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg
     1861 tctgcgatga tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta
     1921 aatccgctaa gcccgatgat gatgggcgta cccagcgtgc tggcccctac cgttgaggcg
     1981 atcgtcgccg taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga
     2041 gccgccagcg gtagctcgac cttacgcagt cgctgaccac gactcattcc catacctttc
     2101 gcaacttctg tcacgctggc atcaatcgct cccagcccgg caagtgtcgc ctgcaggacg
     2161 ggcagcacac cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc
     2221 ggaacggcaa tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt
     2281 tccaccagtg ggcgaaattc cgcgccccac gggcgagtga cagcaattcc ggcaccagtg
     2341 ccaatgatca ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga
     2401 gctgcaaaac tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac
     2461 agcggctggc tgtaaggcag ccagaaaatc agtgccacaa acaaagcaat gagccagaac
     2521 agcggatcgc gcaacatctt catacgctta cgcctcctcc agcagatcct gaaaatgcag
     2581 cgtgccgcaa ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac
     2641 aaacagggag agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg
     2701 ttcttcgcga cgcacgtaat ccgccacact acgtaacgaa agcaggcgca cacccagttc
     2761 gctgcgccca aaaaactggc ggacaaaatc gtttgccgga cgagtcagca tcgtcagcgg
     2821 attgccctgc tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag
     2881 cgcctcatca atatcatgag tgaccagcac aatggtacgc cccagcaaac ggtgaatgcg
     2941 cgtcatctct tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc
     3001 catcagtaag acttgcggat cggcagccag tgcgcgcgcc actcccacac gttgctgctg
     3061 accaccggaa agctgatgcg gataacgctc acgtaaattt gactccagcc ccagtagcgc
     3121 cattaattcg tcgatacgat cgtcaatcct cgcccgcgac catttttgta attgcggcac
     3181 ggtagcgatg ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata
     3241 gcccatccgg cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg
     3301 gatcactccg ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc
     3361 ggagccagat gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc
     3421 gttaacggct ttttgtgcgc cgaacagttt gctgacatgg ccaaattcaa tcattacgtt
     3481 tcaccttcag cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa
     3541 ttaccgggat cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa
     3601 ccagcgcacc aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt
     3661 gcaccatcac cacccgcagg ctgcgcagaa ataccggtaa cgccagtggt aactgaacat
     3721 gcaggaatcg ctgcgccccg ctcatgccca tcgctctggc gctctccagc acatcgcgcg
     3781 ggatctggtt caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca
     3841 gcgcaatcag tgcgggtgtc attccggttc ctgctatgcc gagcgtcccc agccacggaa
     3901 aggccgtaac cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca
     3961 cggtctgaat gacattgagc agagaaaaaa ttgccccctg ccgagcagtg gaaaagtagc
     4021 accagatgcc caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg
     4081 tcagatgttg tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcatta
     4141 gtgagagatc gttaagcgtg ccggagtaca gcaaccacag cggaataatg gcaatctgca
     4201 tatgcaacaa ccagcgccac agcggatgcg tggagattcg gcggatggca tcactacagg
     4261 ccagcaatgc cagcgccgcg gccagccaga aaccactgcc gaggctggta cgcgccagcg
     4321 cactgccatt ttgcgccagt tgggtcgccg cctttcctgc tccccacacc agcaatacga
     4381 agacgaattg cgccagaatg agtgcaaaaa tgctgccttt tttagcggga ataaaacagg
     4441 ctgtcagcca ggcgcaacca acgcccacca gcatccagat cgtttgcggc cacagttgcc
     4501 agagatgacg cccttcaccc gagactaaac gattaggcgc gtaactgata aacggcagcg
     4561 ccgctgcgat tgccgtcagc aacagcagca gcgccagaac aggattaata cgtagataag
     4621 tcacgggaaa ttacttcgtc cacccttttt gtttcaggta gtcggcagcc acttttttgg
     4681 catccagtcc ttccactgca atgctggcat tcagttgctg caatgttttt gcatcgaggc
     4741 tggcgaagac tggctgtagc cactgtgcca tttgcggata ctctttcagc accgactcac
     4801 gcaccactgg tgcaggcgcg tagataggtt gcacgccttt cggatcgctt aaggtttgca
     4861 gccccagcgc cgcgaccgga ccgtcagtgc cgtaagccat tgcggcatta acgccagaag
     4921 tttgctgggc agcggcttta atcgttaccg ccgtgtcgcc gccagccagt gacagtaact
     4981 gatcctgatc gagtttaaag tcgtaggctt tttcaaacgc gggtaacgca tcggcgcgtt
     5041 cgataaactc tgccgaggct gccagtttga aggtgccgcc ctctttcaga taacgactca
     5101 ggtcggcaag cgaagtgagt ttgtttttct ctgccacatc ctgacgcacg gcgatggtcc
     5161 aggtgttatt cgctggtgcg ggcgtcagcc agattaactt gttttgctct gcatcgagtt
     5221 ttttgacttt ctcgtaacct tgctgcgcgt ttttccatgc cgcatcgttt tcatctttaa
     5281 agaagaaagc gccattaccg gtatattccg gatagatatc cagttcaccg gaagtaatcg
     5341 ccccgcgcac cacaggagtc gttccgagtt gcactttatt caccgtcggt acgccgtggc
     5401 tttccagcac ctgcaagatg atgttgccga gtagcgcgcc ttcggtatcg atttttgaac
     5461 cgactttaac gggggaagcc gcctgtagcg gcaggctcac ggctgccaac ataaccagtg
     5521 aacctgccca gacctttgag agtggcatga tgctttcctc attcttttac tgttgttttc
     5581 agcgaattaa gagaaaagca tagttgataa tgacagagtt agcctgagcg gaaggattca
     5641 gttgcagaat cagataaata cttaaagggc atattttgcc cggcgtttct tgccggatgc
     5701 ggcgtaaacg ccttatccgg cctacaaagg tcgaaaattc agcaagttaa gatatgaaca
     5761 tcaggcaaag ggcgcaaacg tcgcgccctt actaaagcat tacagcaact caaactcgcc
     5821 tttcttaacg cgtgcggaat cagtgccgat aaagacattg aacttgccag gttcggcgtc
     5881 atatttcatc tgttgattcc agaacttcag cgcctcgata tcaatcggga agctgacagt
     5941 ctgagtttcg cccggtttca gggtgatttt ctcaaagcct ttcagctgct tcactggacg
     6001 gctcatggaa gccgtcacat cctgcaagta catctgtact accgtggccc cttcgcgttt
     6061 accggtgttc gtcacctgca cgctggcggt cactttgccg tcacgcttca tggtcggcgc
     6121 agaaagtttc acatcagaga cggtgaaagt ggtatagctc agaccatagc cgaacggata
     6181 aagcgccccg ttagcttcat caaaataacg cgaagtgtat ttgttcggct tgtcggcatt
     6241 atacggacga ccggtattca gatggctgta gtacaccggg atctgcccga cagaacgcgg
     6301 gaaggacatt ggcagcttgc cggacgggtt gtaatcgcca aacaatacat cggcaattgc
     6361 attaccgcct tcagtccccg caaaccaggt ttccagaatc gcatcagcct gctgatcttc
     6421 tttcaccagc gccagcggac gcccgttcat cagcaccagc accagcggtt taccggtggc
     6481 tttcagcgcc gcaatcaagt cacgttggct ttgcggaata gtgatatcgg tacggctgga
     6541 ggcctcgtgc gccatcccct gtgcttcacc gaccacagcc accaccacat cagattgttt
     6601 cgccgtctgc accgcttcat caatcatctc ttgcggcgag cgcggatcga ctttgaccgc
     6661 ttcttcatac tgattcagga aatcgataat gcctttgtca ctggtaacgt tcgccccttt
     6721 ggcatacagc actttaccgt tttcaccgac ggcattttta atcccggtca gtacggtcac
     6781 ggattgatcg gcaacaccgg ctgcggacca gctgcccatc acgtcacgtt tactgtccgc
     6841 cagtggccca accaccgcaa tggtggccga ttttttcagc ggtaacgttt cgagacggtt
     6901 tttcagcaac accaggcttt cgcgcgccac ttcacgcgct tctttacggt gcaggcggct
     6961 ttcggcattg gtatccaccg ggtcagactc tttcggcccc aaatggctgt atgggtcgtt
     7021 aaacaatccc atatcatatt taacgttcag tacatggcgg gcagcatcgt ccagctcttc
     7081 catcgtcact ttgccggatt tgatcaaccc aggcagatac ttcgagtaat actcgtcgct
     7141 catactcatg ttgattccgg atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc
     7201 cgtgccatgt ttaatcagct ctttgattgc accgtgatcg gaaacggtga tgcctttaaa
     7261 gccccactgg tcgcgcagaa catctttcag cagccaggaa tcggaggtgg ctggcgtgcc
     7321 gttcagcgag ttcagcgcca ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta
     7381 cggcggcata taatcattaa acaggcgctg cggactcata tcgacggtgt tgtactcttt
     7441 accgccttct accgcgccgt atgcggcaaa gtgtttgacg ctggtcatca ccgagtagcg
     7501 atctgccggg cttttaccct gcatcgcttc caccatggtt ttacccattg ttgaggtgag
     7561 atacgtatct tcgccaaaac cttcggaagc acgtccccag cgcggatcgc gcgagacatc
     7621 gaccatcggt gcccaggtca tattcaggcc atcatctgcc gcttcataag cagagacacg
     7681 tccgaccgtt ttcactgcat cgaggttaaa agacgaggcc agaccgaggc taatcgggaa
     7741 caccgtgcgc tgaccgtgca gcacgtcgta agcaaagaaa agaggaattt tcaggcggct
     7801 taattccatc acctgatcct gcatggcgcg gatatcctga cgggttacgg tgttgaaaat
     7861 cgccccaacc tgaccgtctt tgatcatctc gcggatcgcc tctttcgggt tatccggacc
     7921 gacgctgatt aagcgcagct gaccaatttt ctcatcaact gtcattttct taagcagttc
     7981 ggtgacgaac gcatcccgcg cttcgggcgt taatggatgg ttgccgaata aatcatccgc
     8041 cagtgctggc tgcagggcca gactcaccgc gattcctact gaacatagcc atttcatatg
     8101 gatttttctt cctctgttgc ccgacgaggc agcgaaaatg gtgcaaaaac cgtagtttgc
     8161 cataagcatg atggagagag aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat
     8221 tcactcgctg aattgtcata caaggcgcta ttctagtttg tgatattttt tcgccaccac
     8281 aaggagtgga aaatgtcttc catgacaaca actgataata aagccttttt gaatgaactt
     8341 gcccgtctgg tcggtcattc acacctgctc accgatcccg caaaaacggc ccgctatcgc
     8401 aagggcttcc gttctggtca gggcgacgcg ctggctgtcg ttttccctgg ctcactactg
     8461 gaattgtggc gggtgctgaa agcctgtgtc accgccgaca aaattattct gatgcaggct
     8521 gccaatacag gcctgaccga aggatcgacg ccaaacggta acgactatga tcgcgatatc
     8581 gtgatcatca gcaccctgcg tctcgacaag ctgcacgttc ttggcaaggg cgaacaggtg
     8641 ctggcctatc cgggcaccac gctctattcg ctggaaaaag ccctcaaacc gctgggacgc
     8701 gaaccgcact cagtgattgg atcatcgtgt ataggcgcat cggtcatcgg cggtatttgt
     8761 aacaactccg gcggctcgct ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc
     8821 gcgcgtataa atgaagacgg caaactgacg ctggtgaacc atctggggat tgatctgggc
     8881 gaaacgccgg agcagatcct tagcaagctg gatgatgatc gcatcaaaga tgacgatgtg
     8941 cgtcacgatg gtcgtcacgc ccacgattat gactatgtcc accgcgttcg tgatattgaa
     9001 gccgacacgc ccgcacgtta taacgccgat cctgatcggt tatttgaatc ttctggttgc
     9061 gccgggaagc tggcggtctt tgctgtacgt ctagatacct tcgaagcgga aaaaaatcag
     9121 caggtgtttt atatcggcac caaccagccg gaagtgctga ccgaaatccg ccgtcatatt
     9181 ctggctaact tcgaaaatct gccggttgcc ggggaatata tacaccggga tatctacgat
     9241 attgcggaaa aatacggcaa agacaccttc ctgatgattg ataagttagg caccgacaag
     9301 atgccgttct tctttaatct caagggacgc accgatgcga tgctggagaa agtgaaattc
     9361 ttccgtccgc attttactga ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat
     9421 ttaccgccgc gcatgaaaaa ctggcgcgat aaatacgagc atcatctgct gttaaaaatg
     9481 gcgggcgatg gcgtgggcga agccaaatcg tggctggtgg attatttcaa acaggccgaa
     9541 ggcgatttct ttgtctgtac gccggaggaa ggcagcaaag cgtttttaca ccgtttcgcc
     9601 gctgcgggcg cagcaattcg ttatcaggcg gtgcattccg atgaagtcga agacattctg
     9661 gcgttggata tcgctctgcg gcgtaacgac accgagtggt atgagcattt accgccggag
     9721 atcgacagcc agctggtgca caagctctat tacggccatt ttatgtgcta tgtcttccat
     9781 caggattaca tagtgaaaaa aggcgtggat gtgcatgcgt tgaaagaaca gatgctggaa
     9841 ctgctacagc agcgtggcgc gcaataccct gccgagcata acgtcggtca tttgtataaa
     9901 gcaccggaga cgttgcagaa gttttatcgc gagaacgatc cgaccaacag tatgaatccg
     9961 gggatcggta aaaccagtaa gcggaaaaac tggcaggaag tggagtaaaa attacggatg
    10021 gcatagtatc gccatccgtc attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc
    10081 atttgggcgg ctttttgttt tttatagctc aacgctgctg ctggaacagg catcacttta
    10141 ccggtttcaa tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac
    10201 gcatccatca ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa
    10261 tggcccgccg cattggtaaa accggttttg gttaactgaa tattccagtt atcgcgatac
    10321 accagatggt tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata
    10381 tcttcccggg tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt
    10441 ttggttaagt cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag
    10501 cgcgtgttgt tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg
    10561 taaccaccgg gataatggtg tgcaaggctt gccgccgcgc ggttttctga agacatcagc
    10621 gccagcaaca gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc
    10681 cctttcatct ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt
    10741 gcatccagca caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga
    10801 tccgggtggt tcgagtagat cactttgttg gtattcagat caacaatcat cgcgctaccg
    10861 gaggcaattt caggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt
    10921 gccaaaggca cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg
    10981 atgagcattc agatagtggt tcacgcgcac aggttgcgca ccgccggagt aaggatttac
    11041 tgaggctagc gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag
    11101 gaaatgctgc attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca
    11161 aacgataacc gtagccccat agtataacgg ttagggcgag cagcacttcc agtaccagca
    11221 cgccaatcgc cagcgtcgaa ctagagaagc taaggccttc ctctttgttg atattcagga
    11281 agctcggaat accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca
    11341 gcgcacacaa ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg
    11401 caacgtagcc cgcgaagacc atgcagtgcg caagtgacgg acgctgcgga taattacgcg
    11461 ccatccacca gatgacccgc cccattaccg caaccccagc cagcataacg ccataaaaca
    11521 ggacagccag cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat
    11581 tccagccaat ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca
    11641 aaacgtggtg ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt
    11701 cacgatcggg atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt
    11761 tcatgaacct gacagttcaa gtataagtca gcttgtgatt attttttgtt cgctcatgta
    11821 atttgcaccg tctgatgtac gtcctttttc agcgtgaatg atttccaggg tgtatgctta
    11881 caggcaacca aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg
    11941 gttatgccgc gctggtgatc ggtagcctgg cggaaggtga aaccgtgact ttgctgggag
    12001 gcgttgcggc acatcagggg ctattaaagt tcccgctggt ggtactttct gtggcgcttg
    12061 gcggcatgat tggcgaccag gtgctttatc tgtgcgggcg gcggtttggc ggcaagctgt
    12121 tacgccgttt ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc
    12181 atccgtatct gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga
    12241 cgctgattgg tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg
    12301 catttgcctg ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc
    12361 cgtggttgca caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg
    12421 ttctggtggt gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggatcatc
    12481 aggcgtaaaa ccattgccct gggtaaggcg ttcgcgccgc atccgacatc cggtgcacca
    12541 tgcctgatgc gacgcttgtg cgtcttatca tgcctaccaa aattttaccc attgaaataa
    12601 ccacgcacct cactgcgcta ttctgggttg aactgcggat tcgccaacat aaagccgcca
    12661 tccactatca acgactgccc ggtggtgtaa tttgcgccct ctgagcacag ccacgctacc
    12721 aggctggcaa tctcatgcgt tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg
    12781 ggcttcacgt cgctgtcatc catgccattc attggcgtgg cgatcgcccc aggcgcgact
    12841 gcgttcacca aaatcttatg cctgaccagc tccagcgcca tcgttttggt taacccgccg
    12901 agcgcatgtt tagcggctgt gtaagcgctg gcatccggca gcggcgtatg ttcatgtacc
    12961 gacgtaatgt tgatgatacg accgccctgc ccttgtttca ccatctggcg ggccgcaatt
    13021 tgtgagcata aaaatgcacc atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa
    13081 gccatatcaa gaaacggcgc tttggtcatt gcgcccgcat tattcaccag cacatcaata
    13141 cgccccagcc gttgaatgag tttctccagc gcctgcgcac cttctggcag tttaccgagc
    13201 tccagctgca cgatctccgc acgtacgccg tggctaacta cctcacgcgc ggtatctttt
    13261 gccccttctt catctgagtg ccaggtaatg ccaatatcaa acccctgctg cgccagtaat
    13321 aacgcgcact ctttgccgat ccccgaatcg gaggcggtaa taatcgcaac ctgtgccatc
    13381 aagttctcca cttaacgctg aataaacgtt aagtatagaa ggcgcatatc atcagcgttt
    13441 gtaacccccg cccaacgcac cagtgagttg aatagaagca tccagccact gcccttgcaa
    13501 taacaggcca ttggcccgct cacgcagcgc ggggattctg gcttcgctga cgcgggaacc
    13561 agcaatgatg cccgcgttaa agcgcgcctg cgcaagacct accacacgca aggcatcgcg
    13621 ctcaatttgc gcctgatgct ggtttttctc cgcaagtgtc tgaacctgac tggctgcccg
    13681 cgccacgtca ttcaccgctt caaccaccgc tttgttgtag ctggcgatag acaagttgct
    13741 ttcggctttt gcgatattga gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt
    13801 caggcctgcc gtaacgccca tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa
    13861 cgcatcctgt tgcaggaagg ccatcaggtt gatgtcagga taaaatgccg cttttgccgc
    13921 atcaatggtg cttagcgatg actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg
    13981 ggccagtaag gagtacccca gttcatcagg aagctggctt gccactttcg gcaacgcgac
    14041 cggtttaagc ttcaatgact ttgtctggtt atttgtaagt gcgcttaacc gtgcctcaat
    14101 aattttcatt ttccccgcga catcgttgag ctgctgccgg gttttgctgg cattaatatc
    14161 ggtttccaca ccttcaactg aagaagtaat cccgttctga tatagctggc gatcagtcgc
    14221 gataatggtg ttctgctctt tttctatttg ctgcaagacc gtgtttaacg ccgcctgggt
    14281 ttgccactcc cagtacaggc gggctacgct gccagccagc aattggcggg tttgctcgcg
    14341 ttccgccgcc cgtgctttaa ccgtacccag gcgggcagta acctccgccc gattctttcc
    14401 ccagatatcg agatgccagc ccgccgttaa gccaaaagta ccgttggtgt accacgggcc
    14461 ggtcgtacct gcggccggat cgttcagagc aaacggcccc attaagcctt ctgccgacat
    14521 tttttgccgc tccatatccg ccgaaaagtc gatctgcgga ccatcctgag tggcaactgc
    14581 cttcgcctgg gcttcagcta gctgaatgcg ctgttcagcc acctgcatat ccggtgcgtt
    14641 ctgtagtgca ttgttaatta atgaagtgag ctgattatcg tgatactcca gccaccattg
    14701 gctgtctggc caaccatttt tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcggg
    14761 cgtttgctgg cttaacgcct ggcgggtttc atgcataggc gcacacccgg ccagcatcag
    14821 taacagcgga aaacaggcga tggctggata aaaggaatca cgattcatgg gggaataatc
    14881 aggtaagaaa cggtgcgcgg agattaccgt gtgttgcgat atatttttta gtttcgcgtg
    14941 gcaatacatc agtagcaata aaacgacata cccagaaaaa tatagactaa gtaaatgata
    15001 tcttccgatt tatcttaatc gtttatggat aacggcaaag ggcttcgttt tttcctatac
    15061 ttattcagca ctcacaaata aaggaacgcc aatgaaaatt atactctggg ctgtattgat
    15121 tattttcctg attgggctac tggtggtgac tggcgtattt aagatgatat tttaaaatta
    15181 attaatgtca tcaggtccga aaataacgag aatatttcag tctctcatcc tgttgcgctc
    15241 ctgtcatgtg cattgcttca tataatcact ggcgcaagga gcgcgcaggg ggcggccaat
    15301 cgccgccgcc ccctgcaccc ccgggctctg gcgaacaaaa tcgccgctgc gcggtgccct
    15361 cggcttatcc cttacggcta ccgggtcggg cgcgaggtaa catccctgta aaacgcgccc
    15421 tcagcccaca tccatgtggg ctgccccggc cttcagggaa cgcctcggca attttgacgc
    15481 caccaaacaa ccgtgcggcc tattgataaa gagctaacac attgtcaaaa aacatcacta
    15541 tggtttttta gagtttctcg atatcaattg cctgaatagc ccttgcaata tcaggggaat
    15601 tattcaacac ccgaacatgc tgaaataatt ccgttgcttc atcgtattct ttacgcaaat
    15661 aactcaacca ctgtttaatc cgcgcaacgt gatataaccc ggtatcgccc tgcttttcca
    15721 gacgggtata tttttgcagc aaagcaacca cctccggcca cggcattcgc ggttcgttat
    15781 attttactac ccggctcagg ttgggaatat tgagcgcccc gcgaccaatc atcactgcgt
    15841 cgcagccgct gatcgcaatg cattgttgcg cgctctgcca gtcccagatt tcgccgttgg
    15901 caatcaccgg aatattcagc cgctggcgaa tctcgccaat cgcctgccag tcaatatgct
    15961 ccgcgcggta accctgctct tttgtccgtc catgcaccac cagctccgta gcaccagcct
    16021 gttgaaccgc atcggcgatt tcaaatttct tctcaccgct gtcccagccc agacgcactt
    16081 tcacgctgac gggcaaatgc gccggtacag cttcacgcat cgcttttgca ccctggtaga
    16141 tgagttcagg atctttgagt aacgtcgccc cgccgccgct gccgttaacc gctttcgacg
    16201 ggcagccgca attgagatcc acgccccagg aacctaattc caccgcacgg gcggcgttct
    16261 ctgccagcca ttgtgggaac tgacctaaca actgaacgcg caccagcgta ccggatggtg
    16321 tccggctggc gttttgtagc tcagggcaaa tgcgatgaaa gacttttacc ggcagcagtt
    16381 gatccaccac gcggacaaac tcggtgatgc acagatcgta gtcgttaact tcggtcagca
    16441 attctcgcac cagagagtca agcacaccct ccatcggagc cagtaacaca cgcatatctt
    16501 cacccggaaa aaaatgaggc gctatgttag cgcctatggt caacggatta aaggtctgca
    16561 aagagggatt gctgcaaact gtaatccctc tctatgcttg atgtatgcct gagttcgttg
    16621 cgcattgcgt aatgccgggg agcgaaaaaa taagcgctga tttcttaatg tgatcggtag
    16681 cacgttttac gaattaattg tatgatgaat ccatctcatc tggggtgttg attatgagta
    16741 agacactgaa cattatctgg caatatttac gcgctttcgt cctgatttat gcctgcctgt
    16801 atgcaggcat tttcattgct tccctgctac cggtaaccat tccgggcagc atcatcggga
    16861 tgctgatcct gtttgtcctg ctggccttgc aaattcttcc ggcaaaatgg gtcaatccgg
    16921 ggtgctacgt actgattcgc tatatggcgc tattgtttgt gccgattggc gtaggcgtca
    16981 tgcaatattt tgatttgctc cgcgcacagt ttggcccggt agtggtttcc tgtgcagtca
    17041 gtacgctggt ggtttttctg gtggtgagct ggagttcaca actggtacac ggtgaacgta
    17101 aagtcgtagg tcagaaagga tcagaagaat gatggcgaat atctggtggt cattaccgct
    17161 gacgttgatt gtcttttttg ccgcccgcaa actggcggca cggtataagt ttcccctgct
    17221 taacccgttg ctggtagcaa tggtggtgat cattcctttt ttaatgctga ccggcatctc
    17281 ttacgacagc tactttaaag gtagcgaagt gctaaacgat ctgctgcaac cggcggtcgt
    17341 cgcgctggcc tatcctttat atgagcagct acaccagatc cgcgcgcgct ggaaatcgat
    17401 catcaccatc tgttttatcg gcagcgtggt tgcgatggtg acggggactt ccgtggcatt
    17461 gttgatgggc gcttcaccgg aaatcgccgc gtcaatcctg ccgaaatcag tcaccacgcc
    17521 tattgcaatg gcggttggcg gcagtattgg cggtattccg gcaatcagcg cagtttgcgt
    17581 gattttcgtc ggcatcctcg gcgcggtatt tggccataca ttgcttaatg cgatgcgtat
    17641 tcgtaccaaa gctgcgcgcg gtctggcaat ggggacagcc tcgcacgccc tcggtacggc
    17701 gcgctgcgcc gagctggatt atcaggaagg tgcatttagt tcgctggcgc tggtgttatg
    17761 cgggataatt acttcgctga tcgcaccgtt ccttttcccg attattctgg cagtaatggg
    17821 ctaaaatttg cgatgcgtcg cgcatttttg atgtaagttt cacgcgttgc ataattaatg
    17881 agattcagat cacatataaa gccataacgg gttcgtaaac tgttatccca ttacatgatt
    17941 atgaggcaac gccatgcatc cacgttttca aaccgctttt gcccaacttg cggataactt
    18001 gcaatctgca ctggaaccta ttctggcaga caagtacttc cccgctttgt tgaccgggga
    18061 gcaagtctca tcgctgaaga gcgcaacggg gctggacgaa gacgcgctgg cattcgcact
    18121 acttccactg gcggcggcct gtgcgcgtac gccattgtcg aattttaatg ttggcgcaat
    18181 tgcgcgcggt gtgagcggaa cctggtattt cggtgccaat atggaattta ttggtgcaac
    18241 aatgcagcaa accgttcatg ccgaacaaag cgcgatcagc cacgcctggt tgagtggtga
    18301 aaaagcgctt gcagccatca ccgttaacta tacgccttgt ggtcactgcc gtcagtttat
    18361 gaatgaactg aacagcggtc tggatctgcg tattcatctg ccgggccgcg aggcacacgc
    18421 gctgcgtgac tatctgccgg atgcctttgg gccgaaagat ctggagatta aaacgctgct
    18481 gatggacgaa caggatcacg gctatgcgct gacgggtgat gcgctttctc aggcagcgat
    18541 tgcggcggca aaccgttcgc acatgcctta cagtaagtcg ccaagcggtg tcgcgctgga
    18601 atgtaaagac ggtcgtattt tcagcggcag ctacgctgaa aacgccgcat tcaacccgac
    18661 tctgccaccg ttgcaggggg cgttaattct gttgaatctc aagggttatg attacccgga
    18721 tatccagcgc gcggttctgg cagaaaaagc cgatgcgccg ttgattcagt gggatgccac
    18781 ctccgcaacg ctgaaagctc tcggctgtca cagtatcgac cgcgtgcttc tcgcttaagc
    18841 ctggtgccgg atgcggcgtg aacgccttgt ccggcttgcc agccctctcc tggtgtcgaa
    18901 attcccggca aacagtttgc cgtttcttgc gcaaaaccag cgggtaaagt agcctgatgg
    18961 aaattttcct tagatcgagt ctcctgcatg ttaaagcgcg tgttcctcag cctgttagtc
    19021 ctgatcggct tgctgctgtt gactgtgctc ggcctcgatc gctggatgag ctggaaaacc
    19081 gcgccttata tctacgacga attgcaggat ctcccctacc gccaggtcgg cgtggtgctc
    19141 ggcacagcaa aatattatcg tactggcgta attaatcagt attatcgcta ccgcattcaa
    19201 ggagcgatta atgcctataa cagcggtaag gtaaattatc tattactgag cggcgataac
    19261 gcattgcaaa gttataatga gccgatgacc atgcgcaaag atttaatcgc tgctggtgtc
    19321 gacccatcag atattgttct cgattacgca ggctttcgta cgctggattc catcgtgcgt
    19381 acacgcaaag tgttcgatac taatgatttc attattatca cccaacgttt ccactgtgag
    19441 cgggcattat ttattgcgct gcatatgggg attcaggctc agtgttatgc cgtaccgtca
    19501 ccgaaagata tgctgtcagt acgtattcgc gaatttgccg cccgtttcgg tgcgctggct
    19561 gacctttata tttttagacg tgaaccgcgt tttttagggc cgctggtccc tattccggct
    19621 atgcaccagg tgccggaaga tgcgcagggg tatcccgccg tcacacccga acagttactt
    19681 gaattacaaa agaaacaagg aaagtagtta tggatgtaca gcagtttttt gtcgttgccg
    19741 tttttttcct tatcccgata ttttgtttcc gcgaagcatg gaaaggctgg cgcgcaggcg
    19801 cgattgataa acgggttaaa aatgcaccgg aaccggtgta tgtctggcga gcaaaaaatc
    19861 ccggactctt tttcgcttat atggtggcat atatcggctt cggaatttta tctatcggca
    19921 tgattgttta tcttattttc tatcgttaat attttctctt ttctgttaat tattgagaat
    19981 aattattact tcactcaata agctgcggat ataacattct gaaccgcagc tatttgtgaa
    20041 tcctttcaca gtttaaattt ccccacacgc ttagccttaa tatcagcacg caatatttat
    20101 ttagcgttcg ccttaattac tcatagcatt aaggaagatc acatgccgca acaaaattat
    20161 ctggatgaac tcactccggc ttttacgcct ttactggcga ttaaagaagc ctctcgctgt
    20221 ttattatgtc atgacgctcc ctgtagtcag gcttgcccgg cacagaccga tccggggaaa
    20281 tttattcgct caatctactt tcgtaatttt aaaggcgctg ccgagacaat tcgcgaaaat
    20341 aatgccctcg gtgccgtttg tgccagagtg tgcccgacgg agaaattatg ccaaagcggt
    20401 tgtacccgtg ccggtgttga tgctcccatt gatatcggcc gcttacagcg ttttgttact
    20461 gattttgaac aacaaaccgg gatggaaatt tatcagcccg gtactaaaac gctcggcaaa
    20521 gtcgcgatta ttggcgcagg tcctgccgga ttgcaggcca gtgtgacact gacaaaccag
    20581 ggttatgacg tcacgatcta tgagaaagaa gcacaacccg gtggctgatt gcgtaacggc
    20641 attccgcaat tccggttacc gcaatcagta ctggatgcag agatcgcccg tattgaaaaa
    20701 atgggcgtga ccattaagtg caacaacgaa gtcggtaaca cactcaccct tgagcagctg
    20761 aaagcagaaa accgcgcggt actggtcacc gtggggttat caagcggttc cgggctaccg
    20821 ctgtttgagc atagtgacgt tgagattgcc gtcgacttct tgcaacgtgc acgacaggcg
    20881 caaggcgata tcagcattcc acaaagcgca ttaattatcg gcggcggtga tgtcgcgatg
    20941 gacgtagcca gcacgctgaa agttctcggc tgtcaggcgg taacttgcgt agcgcgtgaa
    21001 gagttagatg agtttccggc aagcgaaaaa gagtttacca gcgcccggga gctgggcgtt
    21061 tcgatcatcg atggattcac gccagtagcc gtggaaggca ataaagtcac gtttaagcat
    21121 gtacggttat cgggcgaact gacgatggcg gcagataaaa ttattctcgc cgtcggtcag
    21181 catgccagac tagatgcctt tgcggagtta gagccgcagc gtaacaccat caaaacacaa
    21241 aattaccaga cccgcgaccc gcaagtcttt gctgctggcg atattgttga gggagacaaa
    21301 accgtggtct atgccgtgaa aaccgggaaa gaagccgccg aggcgattca tcactattta
    21361 gagggagctt gctcatgtta acgaaagatc tttcgattac tttttgcggc gtgaagtttc
    21421 ccaacccgtt ctgcctctct tcttcgccgg taggcaactg ctatgagatg tgtgccaaag
    21481 cctacgacac aggttggggc ggtgtggtgt ttaaaacgat cggctttttt atcgccaacg
    21541 aagtctcgcc gcgttttgat catctggtga aagaagatac cggttttatc ggcttcaaaa
    21601 atatggagca gattgccgag catccgctgg aggagaatct ggccgccctg cgtcggctga
    21661 aagaagatta cccggacaaa gtattgatcg cttcgatcat gggggaaaat gagcagcaat
    21721 gggaggagct ggcgcgcctg gtgcaagaag ctggcgcgga tatgatcgag tgtaacttct
    21781 cctgtccgca aatgacttct catgcgatgg gtagcgatgt cgggcaaagc ccggagctgg
    21841 tagaaaaata ttgtcgggca gtgaaacggg gttccacgct gccaatgctg gcgaagatga
    21901 cgccgaatat cggtgatatg tgcgaagtgg cgctggcggc gaagcgcggc ggcgcagatg
    21961 gcattgcggc gattaacacc gttaaatcca tcaccaatat cgatcttaat cagaaaatcg
    22021 gtatgccgat cgttaacgga aaatcaagta tttccggata ttccggtaaa gcggtaaaac
    22081 cgatcgccct gcgcttcatt cagcaaatgc gcacccatcc agaactgcgc gatttcccaa
    22141 tcagcggtat cggcggcatt gaaacctggg aggatgcggc ggagttttta ttgctcggcg
    22201 cagcaacgtt acaggtcacc accggcatta tgcagtacgg ctatcgcatc gtagaagata
    22261 tggcgagcgg gttatcgcat tatctcgccg atcagggatt tgattcgttg caggagatgg
    22321 taggtctggc gaataacaat attgtcccgg cggaagattt agaccgcagt tatattgtct
    22381 atccccgtat caatcttgac aaatgtgttg gctgtggacg ctgttatatt tcctgttacg
    22441 acggcggtca ccaggcgatg gaatggagcg agaaaacccg cacaccgcat tgtaataccg
    22501 agaaatgtgt gggttgtctg ctttgtggtc acgtctgccc ggtgggttgt attgagctcg
    22561 gggaagtgaa gtttaagaaa ggcgagaaag aacacccggt aacgttgtag aacccgcgtc
    22621 acatccggca tttggttcct gttgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    22681 accacacaga tcatgatgtt atgtgcgttt atgaccgaat gcggaccaca ttcacatcat
    22741 ttcttacgcg cgtatttcag tgaatccagc gctacggcga agataataat cgcccctttg
    22801 atgatgtact gccagtatgg gtttacgccg atatacgtca ggccatagtt gatgacggta
    22861 aaaataatca ccccggtcac cacgccaatc accgtcccca caccgccgct gaacgataca
    22921 ccgcctacca cgcacgccgc gatagcatcc agctcataca taaagccgag gttgttggtg
    22981 gcagagccga tacgtccggc ttctaacatc ccgccaaagg catagaacac gccagacaac
    23041 gcgtagatca tcagcaggtt caggccgacg ttgacaccag atacttttgc cgcttccggg
    23101 ttaccgccaa tggcaaaaat gttcttaccg aagcgggttt tgttccacaa cacccagacg
    23161 aacgccaccg caatcaacgc gtagaaggtg atgtaagaga gacggaaact ccccagcgcg
    23221 acaaagccct gagcaaaggt agagaagcca ctgtcaaaac cagaaattgg cgacgccccg
    23281 acaaagtcgt aatagagcga gttgatgcca tagacgatga tcatcgtgcc gagcgtggta
    23341 atgaacggcg tcacgttgag ataagcgata atcagaccgt tgatcaaacc gatcaccgca
    23401 ccaatggcac agacaatcag aataaccagc gcaatcggca tcgtcgccat ttccgggaac
    23461 actttgttgg cgttatccat ggactgcaat aatgtcgcag ccaccactgc cgccagccct
    23521 acctgacgac cagcagaaag atcggtcccc tgggtgacaa ttaaccctgc cacaccgagc
    23581 gcgataataa tacgcaccga tgactgggtg agaatattac ttaagttcaa cagacttaaa
    23641 aatgttgggt cctggaaaat aataatcgcc agcaaaacta aaagaacgac gtaaataccg
    23701 ccctctttca ggtaagtaag aaaacttttc ttatttaacg cactcatggg aagcccctaa
    23761 tcttaaaggt gcaaagacgc aagacgcaga atttcgtttt gcgttgttgt ttttgtatcg
    23821 acaattccgg aaacgagacc attgctcatg accagaatac ggtctgttat ccctaacaac
    23881 tcaggcattt cagaggagat aataataatc cccttgcctt tcttcgccag ttcggcaatt
    23941 aactgataaa tttcaaactt cgccccgaca tcaataccgc gcgtcggttc atcgagcatt
    24001 aatatttctg gttgcgttag tagccagcga ccaataatca ccttttgctg attaccaccg
    24061 gagagcgaac caatttgcgt ccgatgaccc ggcgttttta cccgcatcga atcaatcacc
    24121 cactgggtat cgcttttcat ccgcgagtta tccagtaaac caactttatt tttgtagttg
    24181 cgaatattgg aaattaacga gttaaaacca atatccagat aggcataaat tcccgttgag
    24241 cggcgctcct cagttaccag tgcaaatccg tggtttatgg cttcgttggc attatggtta
    24301 ttgatctgtt tgccgtgcaa cgtaatggtg ccagccgatt tctcgcgaat accaaataac
    24361 gtctcaacaa tatcggtacg tttcgccccc accagcccgg caataccgag gatctcccct
    24421 ttatgcagat caaacgagac atcgcgaatc gacggctggc gcagtgacgt taggttacgc
    24481 acctcgagga tgacttcgcc cggcttgttt tctttgtcag gaaaacgctg attaagagaa
    24541 cgcccaacca tcatggcgat gatcttgtcc atcgtcagtc ctgccagcgg ttcggtggcg
    24601 atccactgac cgtcgcgcaa tacggtaact tcatcacata actggaagat ttcttccatt
    24661 ttatgcgaga tataaacaat accgcagccg cgctctttta atttacgaat aatagtgaac
    24721 agatgattga cctctttttc ggttaacgaa gaagtcggtt catccataat cacaattttc
    24781 gcgttatagg aaaacgcttt ggcgatttcg atcatctgca tttgcgaaac ggataatgtg
    24841 ccgacgcgcg cacgcggatc gatatcaata tccagttcat caaaaatcgc tttggtttcg
    24901 cggtacattt tgtcctgatc gacaaacatg cctttggtgg gatatcgccc cagccacatg
    24961 ttatccatca ccgaacgttg taataccagg tttaactcct ggtgtaccat cgaaatacca
    25021 ttttccagtg cctctttggc ggaatggaaa tcgatctctt taccctggaa taaaatggtg
    25081 ccggagtctt tttgataaat accaaacagg cattttaata atgtcgattt tcctgcaccg
    25141 ttttccccca ttaatgcatg gatagaatgt ggccggaatt ttaaattaac gttatcaagt
    25201 gccttaacac caggaaagga cttgttgata ccgctcattt ccaacaagta ttccccggaa
    25261 gacggagtcg ttgagctgac catataattt taccttgttg gccatacaat aagggcgcag
    25321 taaacgactg cgcccaatca gtcttatttc ttgctgaatt cagccaggtt gtctttatct
    25381 acgccaacat aaggtacgcg gaccactttg ttgtcgattt tccagttggt gccatcagcc
    25441 gcacctttgc catcggccag gtttttcgcc agatcaaagg tcgctttcgc ctggttgtta
    25501 gcatcgttca gtactgtgcc cgccagtgca ccggatttca ccagcgccag cgcttctggc
    25561 agcgcatcga cgccaaacac cggaatgctg gacttgttgt gtgctttcag tgcttctacc
    25621 gcgcccattg ccatcgcatc gttgttggcg ataaccactt cgattttgtt ggcgttcggg
    25681 ccagacagcc aggcgtccat cttatctttc gcctgagcgg tgtcccacat tgcggtatct
    25741 aactgtaact gttcagtttt gatgccttta tcgttcaatt ctttaatcac gtaagtggta
    25801 cgtgcttctg catccggatg gcccggttca cctttcagca gtacgaactg aatctgaccg
    25861 tctttgttca gatcccaacc ctgattcgcc gcccagtgtt tagcaatcaa atcgccctga
    25921 ataatgccgg actctttgga gtcagtgcca acgtagtagg ctttgtcgta gctatccagc
    25981 gccttacgag acggttcttt gttgaagaaa accaccggca cgttttgccc acgcgctttc
    26041 tcaatcaccg tacccgcagc tgccgggtca accaggttga ttgccagtgc cttcacccct
    26101 ttcgccagca atacgtcgat ctgatcgttc tgcttggact ggtcattctg agaatcattc
    26161 atcagcagct gaacatctgg cgcggctttc gcatcttgct caatagcctt gcgcactaca
    26221 gacataaagt tatcgtcgta cttatagatt gttacaccaa tgcgagtatc agcagcgtgt
    26281 gcagcggcac cgaataacat gctggccatc acagcagaca gggttaacac cttcttattc
    26341 atggtatctc cggtttttct tatgcagggt agtgcttgag ataaatgctc ggcggggcag
    26401 tagagttaat gaagtgttac tgaacgccga agctcacttt tttaaattcg ttcttccatg
    26461 cccggtaacg ctccagaaaa cggctttaat tgttgttatg acgctattac ctcggtgaaa
    26521 gtgattattc actgttacat acgggttaca acgttaaaac ggtgcaatca tagctatcac
    26581 attgttaaga tactgtgaaa tcactcacag attgaaagcg gttacatcgc ctgatttgtt
    26641 gagttagtga tcgccgccgc attctggcgc gttgcgacag aatggcgacg cactaacgtc
    26701 ggcataaaac agtggctggc acgaggatca atattgcctg ctgccccctg caaggccagt
    26761 tcggtggcta atttagccat tgaagcaatg ggataacgca cggtcgttaa ttgcgggtcg
    26821 gtgtaacggg cgatgggaat atcatcgaaa ccgatgattg agagatgtaa cggaatcgca
    26881 atgccattat cttttaatgc tgtcagtgcg ccagcggcca tattgtcgtt ataagcaaat
    26941 atagcggtaa gttgtagatt gcgccccagc agttcaacca tcgccgcctc accgcccggc
    27001 atgtccggcg taccagtgcc aatccagctt tccggcggaa taatatcctg ctctttcaac
    27061 gcactcatcc agcctgcttt acgcatggcg tcatcttcaa tgccgtgact ggaagaaaga
    27121 taaccaatac gttgatgacc gttattcagc aacatgcgcg tcgccattcg ggcaccgctg
    27181 agattatcca ggcaaacgca acgatgggcg taccccggca caacgcggtt gattaacacc
    27241 ataccgggaa tgttatccat aaattgcgcc agttcatcgt cactcaatgc ttttgagtga
    27301 acaatcaacg cattacaacg ctggcgaatt aacacctcaa tggcgtgacg ctctttttcc
    27361 gcttcatgat agctattgcc gattagcacg tatttctgat gctgctgagc gactagatcc
    27421 accgctttta ccagcgcgcc gaaaaacgca tcagaaacgt ccatcaccac cacgccaatg
    27481 gtgtcgctaa cctgagttgc cagcgcctgg gcattggcgt ttggccgata atccagctca
    27541 ctcacggctt tcattactgc ttcacgcgtg tcggcactga ccagcgtgct gttattgagc
    27601 acccgggaaa ccgttgccac agagacgcca gcctaacgcg ctacatcacg aatggtgatc
    27661 atattcacta cctgttatga gattgcagta aatgactgct tgctggcggc tattttgtca
    27721 gcactgaata caggacttcg tgaatcgagt cacagcaatg gaaacggtta cagccgtttt
    27781 gttaacgaat gtgatccaga tcgttatctt gatgttttag atattgccgt tccggcagca
    27841 cgcaaagtta actgacgcca gagccattcc accggcccct ggcggaagta acgcagccag
    27901 ataacagaga agaggatatt cgccagccat accggaataa caaacgccag cagctccagg
    27961 cggtcaaaat gcataaacaa accgaggtgg taaaaaagca tggtacaaat cagcgtttgc
    28021 aatagatagt tggtcagcgc catccgtccg acgcaggcga tcgcaagcac cagcttaaag
    28081 cggctcaatt gcggccagaa gccataaaac agcgacgcat agccaatcgc ctgaaacggc
    28141 gcactcagtt cccgcggcat ttgaagtaag aaggcacacc agcgatacgc ccagtccagc
    28201 tgccattgca gggcgatggc aggaaggtta atggtcaccc caatcgccac cagcacaaaa
    28261 ccagtacgac gatagtgacg taagctgaac tgccctttca gccagccgct gcgcatcagc
    28321 gccgcaccaa tgagcatcat ccctgccagt tgccagccat actgtgcgcc cagtgccagt
    28381 aaactgttgc caacaccatc ggcacgatta ctgatcgctt caacgccgcc gtgaagcttc
    28441 cagtattttt catacaaaat agccgatgca tccggcgtcc aggtgcggct ggtctggctg
    28501 tcggaaatca gccccaataa cagcaaaacg ccaagcccca ccagataaag catgacgcct
    28561 gtattaaaca ggctttttac cgatggcgca tcgcgcacca gccgccagca gattaagccc
    28621 accagcccgt aagccagcag aatatcgccg tcccagaaca ataaaccgtg aataaaaccc
    28681 agcaagacta acagcgttaa ccgcgactgg atccagcgtc tgccacgggg cagcaacatt
    28741 tgcaggcccg caccaaacag cagcgcaaaa agcgtgagga atttcacctg gccgatgaga
    28801 tcgagaaatg cccaggtcca tgcatcctgc ggcgtaatag cgccgtacca ggcgggattg
    28861 agataagccg cctttggtag cccaaaggcg ctgatgttta atagcaggat ccccagaatg
    28921 gcgacgccgc gaacaaaatc gagcgttacg ttgcgctcca tggttcctgc cttttaatca
    28981 gttgtgatga cgcacagcgc gcagaaactc gtggcgcgta ttctgactgg atttgaacaa
    29041 tccaccaaga gaggtcgttg ttgtggcact ggttgcatcg cggatgccac gcgctttcac
    29101 gcagtaatgc accgcatcaa tggagacagc cacgttattg gtgcccagca gcgtttgtag
    29161 cgcaataaga atttgctgcg tcagacgttc ctgcacctgc ggacgctggg caaagaactg
    29221 cacgatacgg ttaatttttg acagaccgat caccgaatct ttcgggatat aagccaccgt
    29281 cgctttgcca tcgatggtaa caaaatggtg ttcacaggta ctggtcagag tgatatcgcg
    29341 cacggtgacc atttcatcga ccttcatttt gttttcaatg agggtgattt tcgggaagtt
    29401 ggcgtaatcc agaccggaga aaatttcatc gacatacatt ttagcgatgc gatgcggcgt
    29461 ttccatcaaa ctgtcatcag ccaggtcgag attcagcagc tgcatgattt cggtcatatg
    29521 accagcaata aggcttttgc gcgtttcgtt atccatttca tgcacgggcg ggcgcagcgg
    29581 tgtttccagt cctcgcgcaa ctaacgcttc atgaaccagg gccgcttctt tactgagtga
    29641 tggcatttat gatttctcct gcaggtgtga cgcctccgcc ctgcgtgggg gcaaagttat
    29701 taagctgatt tacagcctga ttattgtgcg tgaggcggcg cacataatcc agtattcaca
    29761 gcgataatta ttgtaattgc cgctgacttt catcagcaga tgttaaaaca tcgttatgca
    29821 aatacggaag tgaaagttac tcacagcaca ttgaataaac ggtatgatga agaaattgca
    29881 aacaacacaa caaggagcca cgcatggaaa tgctcgaaga gcaccgctgt tttgaaggct
    29941 ggcagcaacg ctggcgacac gactccagta ccttaaactg cccgatgacg ttcagtatct
    30001 ttctcccacc ccctcgtgat cacaccccgc caccagtgct gtactggctt tccggattaa
    30061 cctgcaatga cgagaacttc accaccaagg cgggtgccca gcgcgtggcg gcagaactgg
    30121 ggattgtact ggtgatgcca gacaccagcc cgcgcggcga aaaggttgcc aacgacgatg
    30181 gctacgattt aggccagggc gcaggctttt atcttaatgc cacgcaaccg ccgtgggcga
    30241 cgcattaccg gatgtatgat tatctgcgcg atgaattacc ggcgctggtt cagtcgcaat
    30301 ttaatgtcag cgaccgctgc gccattagcg gtcactcaat gggtggtcac ggtgcgctga
    30361 ttatggcgct gaaaaatccg ggtaaataca ccagcgtttc ggcctttgcg ccaattgtga
    30421 atccgtgcag cgtcccgtgg ggaatcaaag cgtttagcac ctatttaggt gaggacaaaa
    30481 atgcatggct ggaatgggat agttgcgcgc tgatgtatgc cagtaacgcg caggatgcga
    30541 tcccgacgct tatcgatcag ggcgataatg atcagtttct tgccgaccag ttgcaacctg
    30601 cggtattggc agaagccgcg cgccagaaag cgtggccgat gacgctgcgt attcagccgg
    30661 gatatgatca cagttactac ttcatcgcct cttttataga ggatcacctg cgcttccatg
    30721 cgcagtattt actgaagtga aagtccgccc ggttcgccgg gcatcttctc atcagaagcg
    30781 ataatccact gccataaagt aacgacgtcc gtcttcgtta tagctgtagt cgtcacgact
    30841 gagatccttg tcgccaaggt tcagcacgcc tgcgcgcagt ttgacgtctt tagtcacctg
    30901 ccaggccgcg ccggtattcc agatggtata accgcccggt gttttagccg tcgcgctgtc
    30961 ggcgcgtttc tgcccggtat agtgaccaga aacatagaat gaccagtctt ccagcgccag
    31021 cggtttccag tccagcgtac cgttagcagt atggaacggc agatcggata gcggtttgtt
    31081 ttcgccgttg ctgacatcac gaccatcgtt gtaggtgtag ttgatcgaca gtttccattc
    31141 atcgttgaac ggaattttca gttcggtttc cacgccctga atacgagctt tgttaacgtt
    31201 gtagtagcta aataccggta tgcgccgtcc gttagcgccc gtctcaaaac caacaaagtt
    31261 ttggtagccc ggtgcagcgt tgacgtcaga cgtacggctg atgctgatac gatctttcac
    31321 atcgttacgg aaaacggtaa cgctggattc aaccccttcc agccagcctt cttcacccat
    31381 gtagtaaagc cccagctccc aactttcgct ggtttctggt ttcagatccg ggctacccac
    31441 aatcttacat gcgccacggc aggaattgct cgtccagtca gggctaagtt gcaacagaga
    31501 aggtgcttta aatgccgtcg cccagccccc tttcaccgtt acggtgtcgg tggcgttata
    31561 aaccaggtag gcacgcggac tccagtgttc accgtaggtt tcgtgatcgt ccatacgcac
    31621 gccggtcgtc agcgccagcg gctcgaagat ccgccattca tcttccacaa acagcgcgta
    31681 ctggctggca gacgttttgg agctggttcc cccggtcagg ttcaccgcat cgctaagttt
    31741 gtcgtgacgc cattcaccgc caaccgtgag aaactgatta atcgccgtca gcggcaacgt
    31801 gtatttgccg tcgaccgtat tgctttcgga agttatcggg ctgctgttgc cagggttttt
    31861 gttctcgact ttctcaccgt agtatttcag ttcgctggtg ccgtaatccc aacgcccatt
    31921 atggctgacg gagtagttct ggcgttccag gcggtttttg tccagcgagt cggaatcacg
    31981 atcctgacgg tcgaaaccgt atccggcagt aaaatcgtga ttttgattcg gtgtccaggc
    32041 aaattcgaca ttgccgtcgc ggctggagaa tccttcaata cgcggcgttt ctccggtatc
    32101 ggtggtcgtt gagttttgcg ggtcatcctt ttcacgtttt gccaggctgc cgtaagcttt
    32161 cattcccagc acaccatcaa ttaatggtcc actggtaaag aactgaccgt tataggtatc
    32221 accgcgatcg cgatgttcct gaatggtggt atcgacggta acggtacccg accatttctg
    32281 accgattttt ttggtgatga tattcactac accgccgagc gcatcggaac cgtacagcga
    32341 cgacatcggg ccacggacca cttcaatacg ttcgatggaa tcgaccggga tccagttcag
    32401 atcgaaatca ttgtggcgga agacggcatt gcgggagttc acgcgtttac cgtcgacgag
    32461 aatcagggta tagctgctgt ccagaccacg aatactaacg cccttacggt tatccccttc
    32521 gttcgtcagt tgtacgccag gcacttcttt gaggacatcc ttcagattct gtaccggttt
    32581 tcgctgcaga tcttcctggg taatgacgct gatactggcg ggtgcatctt taaggttttg
    32641 ttcaacggaa gatgcagtga caaccatcgt ttcgccatcg tcatcgaccg ctaacacagg
    32701 ccatacacaa gaaatagcgg acaaacacag cccgacccgt acgaaagggt tcaacctaaa
    32761 cattccatat ctccatgagg taactacgaa aataaaatgg gtatcgctca catcttcttt
    32821 acgtcccctt gcgtacggca gcatcgcggt ggacttattt gtatgcaggt gatcatccca
    32881 gaaagccgga ccagcctctg atttgtcagc cttttttgat tgtagcgtaa cgataatgca
    32941 aacgataaca gttatcaatc caaatgttaa attttatatc cgcatgattt gtcagggaaa
    33001 tggaattgaa aaagccctct cggttgagag ggcttagcaa ggaagggagg aaacttattt
    33061 cttatcgttc tgcgggaact tcatttcgct gtagcgtacg aagtgagttc ctttaatcag
    33121 cttgtagccg aaccaaataa tcaggaacag cgggatacca atatacgttg ccgctacgcc
    33181 gccccagtca atggtatctt tcaggaacgc ttcgtagttc tggcccaaag tgataatcag
    33241 acacagaatg aatgcgaaga tcggccccag tgggaagaaa cctgaacggt acggcagatc
    33301 gttaatgtcg tgtccctgca atacgtaacc gcgacggaag cgatagtggc taatggcaat
    33361 ccccagccag gcgataaaac ccgtcatccc ggaggtgttc agcagccaca ggtataccgt
    33421 ctggttgcca aacatggagg tcaggaagca caaaccggca atcaccgtcg tcgcatacag
    33481 cgcattacgc ggcacgccac cacgcgacaa tttagcgaaa atgcgcggcg ctttaccgtc
    33541 acacgccagg gtgtacagca tacgagtaga cgcatacata ccggagttac ctgctgacag
    33601 caccgccgtc agaataactg cgttcatcac cgccgccgca gagagcagac ccgcgtgctg
    33661 gaacaccaag gtgaacggac tgacgctgat gtctttaaca tcgttacgca gcaggctcgg
    33721 atcggtatac ggaataatca ggctgataat caggatcgcg aacacataga acaacaggat
    33781 tcgccagaat acctgacgta ccgcgcgtgg aatgtttttc gccggatctt cggactcgcc
    33841 tgcagcaata ccgatcagct cggttccctg gaaagagaag ccgacaatca tagctacgcc
    33901 gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt tgctccagcc
    33961 cgcaggctgc gcgcctttga agataccgat aatcatcagc acgccaacga tgataaagac
    34021 aataactgtc gtgactttga tcagtgaaaa ccagtattcc gcttcaccaa agccacgaac
    34081 tgagatgtag ttcagcagga agataacgcc gaggaacaac gcactccaga tccagcccgg
    34141 tgtatccggg aaccaccagc tcatgaccag ctgtgctgca accaagtcaa cggcaatagt
    34201 caccgcccag ttgtaccagt agttccagcc cagcgcgaag ccaaagcctt cttcaacata
    34261 gttctgaccg taagtggcaa acgaaccgga aaccggcata tatgcagcca attcaccgag
    34321 actggtcatc aggaagtaaa ccatcaggcc aatcagcata tacgagagca atgccccgcc
    34381 cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac cgatggaacc
    34441 gccaatggca atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc ccggcgcttc
    34501 tgtagtttta gtttcggaac ccatacaaaa atgctatcca tcttaaaaat gaggcgcgat
    34561 tgtagcagac gattggcaat ccttccggca gaaatacacg gttataagac accttcatga
    34621 tcgcccaggg attataagta aagcagccaa tctcttttct ggcgaatgca tgctgagggc
    34681 gctgcgtcac gaaatcgaca cagcaccagc atgttcttgt acagcacctt aacgcggtac
    34741 attcgcggga tcgcaatagt ccagaaagcg ccgtagcgcg ttggaaaggt gtttttgccg
    34801 atgatgtata cgccacaacg tacgcatcag gcgcgggaga gggaccgcaa cttcacttaa
    34861 tgtgcctgcc tgcaattgat cttcaatcac acgtcgcgac aggcaactaa ttcccaaccc
    34921 atgacgcacc gcatgtttga ttgcctcgga gttacctaat tccatcgcca tctcaaactt
    34981 cggtaaatgt gacagcaaca gataatcgac aatctcccgc gtgccggaac cgcgttcacg
    35041 caggatccac ggcgcagcgg ccagctgttc taaggtgacc ggaccacgag ccaacggcga
    35101 agtcggcgcg gcgaaaacca ccagctcgtc ttccagccac ggttcagaaa tgatttcagt
    35161 gctgtggcac ggtccttcaa taaagccaat atcaacgcgg aaatccagca ccgcttgcat
    35221 cacgtcctgg ctgttcccaa cgctaagttc aatcggcaac tgcggataat catggcgata
    35281 acgggcgata actgcaggca gaatgtagtt accgatggta ctactggcat agatacgaat
    35341 cgcgccgttg tcttcgcgaa acagttgttc gatttcaacc gcctgttcaa gcaatgccaa
    35401 cgcacgcgga tagagcagcc gcccgtgttc attaacaacc agtcttttcc ccacgcgatc
    35461 aaacagttgc acgccaagct gcccttccag gtcggtcaag gctgcgctca ctgctgattg
    35521 cgacaacgcc agcatcaccg acgcctgggt ggttgatcca cttttcaata cttctgcaaa
    35581 aacttccaac tgccggaggg tgatgtgcat agtcgcttac cacttataaa gattaattat
    35641 aaatatataa tcaattttat ttttaaacca gttagtcgta accttatgcc cggttaaagg
    35701 agagggttat gacgaatatc accttacaga aacaacatcg tacactgtgg cattttattc
    35761 cggggttagc cctgagtgca gttatcaccg gggtcgccct gtggggtggt tccattcccg
    35821 cggttgcggg tgccgggttt agtgccctca ccctcgcaat cttgttgggg atggttttag
    35881 gcaacaccat ctatccgcac atctggaaaa gctgtgacgg tggtgtgctg tttgccaagc
    35941 aatatttatt acgtctgggt atcattcttt atggcttccg tctgacgttc tcgcaaattg
    36001 ccgatgtcgg tatcagtggg atcatcattg acgtgttgac gctgtccagt accttcctgc
    36061 tggcttgctt cctggggcag aaagtgtttg gtctggataa gcacaccagc tggttgatcg
    36121 gtgccggtag cagtatctgt ggtgctgccg cggtactggc gactgagtcg gtagtgaaag
    36181 cggaagccag taaagtaacc gtggctgttg caaccgttgt tatcttcggg accgtcgcga
    36241 ttttcctcta cccggcgata tatccgctga tgtctcaatg gtttagtccg gaaactttcg
    36301 gtatctacat tggttctact gtgcacgaag tggcgcaggt ggtggcggca ggtcatgcca
    36361 tcagcccgga tgcggaaaac gcagcagtta tttccaaaat gctgcgcgtg atgatgctgg
    36421 ctccgttcct catcctgctg gcggcgcgtg ttaaacagct gtctggggcg aacagcggcg
    36481 agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt catcgtcgtt gccatcttta
    36541 actcgttcca cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg gataccttcc
    36601 tgctggcaat ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg ctgaaaaaag
    36661 ctggggcgaa acctctgctg atggcactgg tactgtttgc ctggctgatt gttggtggtg
    36721 gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac cactacatct tgctcctgtt
    36781 aacccgctat cattaccgtt ttcctccagc gggtttaaca ggagtcctcg catgaaatac
    36841 attggagcgc acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg cgccgccgaa
    36901 atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg tgccgcaccg
    36961 ctcacgacgc aaaccatcga tgaattcaaa gccgcctgtg aaaaatatca ctacacatcg
    37021 gcgcaaattc ttccccacga cagttatctg attaacctcg gacatccggt cactgaagct
    37081 ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca actggggctt
    37141 tctttgctca acttccaccc tggcagccat ctgatgcaga tttcagaaga ggattgcctt
    37201 gcgcgtattg ccgaatccat caacattgcg ctggataaaa ctcaaggtgt aacagcggtg
    37261 attgaaaaca ccgccggtca gggcagtaac ttagggttta aattcgaaca tctcgcggcg
    37321 attatcgacg gcgtggaaga taaatcccgc gtcggcgtct gcattgatac ctgccatgct
    37381 ttcgctgccg ggtatgattt gcgtactcca gccgaatgcg aaaaaacgtt cgctgatttt
    37441 gcccgtattg tcggctttaa gtatctgcgc gggatgcacc ttaacgatgc gaaaagtacc
    37501 tttggcagcc gcgttgaccg ccatcatagc ctcggtgaag gcaatatcgg tcatgatgcg
    37561 ttccgctgga tcatgcaaga cgaccgtttc gacggcattc cgctgatcct tgaaaccatc
    37621 aacccggata tctgggcaga agagatcgcc tggctgaaag cgcaacaaac tgaaaaagcg
    37681 gtggcctgat cgatgaacga cagggaaaag cagattctta aaattttacg gcgtaacccg
    37741 ctgattcaac agaatgaaat cgccgatatt ctgcaaatca gccgctcacg tgttgccgct
    37801 catattatgg atttgatgcg caagggtttg attaaaggca aaggatatat ccttaccgag
    37861 caggattact gtgtggttgt gggtgcaatc aatatggata ttcgcggaat ggcggatatc
    37921 cgttacccgc aagcagcctc tcatccaggt tctgttcact gttccgcagg cggcgtcgga
    37981 cgtaatatcg cgcataacct ggctctgctt ggtcgggatg tgcatttaat atcagctatt
    38041 ggcaacgatt tttacggcga gacattactg gaagaaacgc gccgcgcggg cgtgaacgtt
    38101 tcaaactgca ttcgtctgca tggacacagc actgcaactt atctcgctat cgctaataag
    38161 caggaagaaa ctatcctggc aatcaatgac acacatattc tgcaacagct aacgcctcaa
    38221 ctgctcaata cttcacggga cttgattcgc catgctggtg tggtgctggc tgattgtaat
    38281 ctcacacctg aagccctgga gtgggtcttt accattgctg atgaaattcc gatgtttgtc
    38341 gataccgttt ccgaatttaa agcgaacaaa gtaaaaaact ggtacagccg gatccacact
    38401 ctgaaaccaa cacaaaatga actggaaatt ttgtgggggc aaccgattaa agacgacaac
    38461 gatcgcatca gagctgtaaa ttcgctgcat caacaaggtg ttaaacgcat ttttgtttat
    38521 ctgaaagatg aatctgtatt ttgcagcgat aaggatggag aacaattttt gctgaccgcg
    38581 ccagcgcata cgacagtaga cagttttggt gctgacgatg gttttatggc gggcctggta
    38641 tatagctttc ttgaaggata cagtttccgc gacagcgccc gttttgcggt agcctgcgcg
    38701 gcaatttcgc gcgccagcgg cagcttaaac aaccctaccc tgtctgccga taacgcgctt
    38761 tcattagtgc caatggtgta acaatgttgc cggattcgac gcttgaccgc atcttatccg
    38821 gctcataaat tgcgcactac gctaaaccaa taaagaatcc tgcaatagta gcactcatca
    38881 ggttagaaag tgtcgccgcc gccagcgcgc gtaaaccaag ttgggcgatt tccggcgcac
    38941 ggtgtggcgc aaccgcagaa aacgccccca ccaccacccc gatagaacca aagttagcga
    39001 aaccgcacaa cgcgaaagaa ataatcgcca cggttttagc atccagagtg ccacccgttt
    39061 gcagatatgg cgagaaattg agataagcga caaattcatt gatcgccagc ttctgcccaa
    39121 tcaaactccc ggcaagattt gcatcactcc agtcaacccc catcacccac gccaatgggg
    39181 ccaacaggta acctaaaatc gactccagcg aagcatgttc aaaaccaaac cagccgccaa
    39241 caccgccgat aataccgtta atcaacgcaa ttattgcaac aaatgccata acaactgtcg
    39301 ctacaccggc ggcgattttc agcccggtca ttgcccctgt cgcagcggct tcaataatgc
    39361 tttttggcgg tgtttcggtg aaagagaggt tattaaaaga aacctgtgaa gattccgtag
    39421 ccgggcttaa caggcgggca aacaagatcc cgccagggat cgccattaac gatgccgcca
    39481 gcaaatattc cacaggtacg cccagtgcgg cgtaaccaat cattgtcgaa ccagcaatcg
    39541 aggccatgcc actacaaatc gctgtaaata attcattgcg attcagacga tcgataaagg
    39601 gtttgacgat cgccggaatt tcgttttgcc cgaggaaaat ggtggtgacc gcgacgaatg
    39661 actcgatctt gctgatattt aaagctttct ggaagatacc gccgagaatg cgaattaaga
    39721 tccccatcac accgatgtag tagagaatac tcaccagtgc agtgacgaag ataattgccg
    39781 gtaataccct gaaaccaaag ataaatcctg cgccatcaaa taaggtatcc attttcggtc
    39841 cgaccagaga accgaagata aatgcgctac ctgcgtcgct gtacgccatc actttgtgga
    39901 cgccaaaagc gactttttct gcgacccaac gccctggcgg taaccaaagc ataatcccgc
    39961 caatcacgac ctgtaacact aacgccgcgc caacggtacg caggctgatc ttcttcttgt
    40021 ttactgacag taaaaacgca attgccagca gtaccaccat tcccagaaca cttctcatga
    40081 catccatagt gattatctct tcatgtcatt ttgggcgacg gctttctgcc catcagaaat
    40141 agccgggcct gcatcttgcg atactgtccc ggcttgttat caacacttaa tgggttttta
    40201 tgtagccgcg tacgcactct tcgactaatc cccagaacca gtcggtatca atagtgatgc
    40261 cgactttagt attggcgggc ttgccaagaa cgcccagctc gtcgcagacg gtacgcccat
    40321 agcaagggcc actgttgacg tccacttcga cgtacatctc ctgggtttta atgccatcag
    40381 ggttaatcag ataaccgatg caggtggcgt cgtgcaccgg gccgccagcc aggccgtagt
    40441 tttcgaactg cgttttgaga gtgaagttca tgatgtcgct gaacatctct ccggcagggc
    40501 cgcctgccct ttccattcgt gcaattacgt ccggggtgca aacggtctgg ttggtaagat
    40561 cgaggcccat catcactaat ggaacgccgg aggtgaacac cacgcgtgcg gcttccgggt
    40621 cggcaaagat attgaactcg gcagatgggg tgaagttgcc tgtaccgtaa gcgccgccca
    40681 ttagcacaat ttcacggatt ttgggcagga ttgcgggttg catacgcatt gccaccgcga
    40741 tatttgaaag cggaccaacc ggcaccagag tgatatcgcc atcgcttgcc ataagggtat
    40801 caatgatgta tttcaccgcg tgggtacttt ctgcctggcg ggtcagcggc tcgaataccg
    40861 ggccatccag tccggtttca ccgtgaatat tatcggcaac gatttgttga cgcataatgg
    40921 gttgcggcat ccccgcataa accggaacat taatctccag tttctggcaa acattcaggc
    40981 cattaattaa tgttttatca agcgtctgat tacccgcaac aatggtgata cctaataaat
    41041 caatcgccgg atgtttcgcc gccatcatta tagcaatagc atcatcatga cccggatcac
    41101 aatccagaat aatttttctc ttttccattg tttatttcct ctgtttccag ttgcgttatt
    41161 ttttctacag caacgaaatt tcgatcgggc cgatgattga atcttaacaa cagcgtacga
    41221 atgctaaata tgagaaatct catagcggat aaacatcgtg aaagaaatcc acaataatga
    41281 tcttaagcag caattgatga gtgaatccgc gtttaaggat tgctttttaa cggatgtttc
    41341 agccgatacg cggctgtttc attttttagc gcgtgactac attgtgcagg aagggcaaca
    41401 gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg ctttacgcca cgctagctaa
    41461 tggtcgcgtg tcgctgatcg atttctttgc cgccccctgt tttattggcg agattgagtt
    41521 aatcgacaaa gaccatgaac cgcgtgcggt gcaggctatt gaagagtgtt ggtgccttgc
    41581 gctccctatg aaacattacc gcccgctgtt attaaacgac acgctatttt tacgaaaact
    41641 ctgcgtcacc ttaagtcata aaaattatcg taatattgtt tctttaactc agaatcaatc
    41701 atttccgtta gtgaatcgcc tggcagcatt tatattactc tcgcaggaag gtgatcttta
    41761 tcacgaaaag catacgcaag cggcagagta tttaggcgtt tcttatcgac atcttttata
    41821 tgttctcgcg caattcattc acgacggttt attaattaaa agcaagaaag ggtatctaat
    41881 taaaaacaga aagcagttgt caggactggc gctggagatg gacccggaga ataaattctc
    41941 cgggatgatg cagtaaaaat tattggcaat agcacgattg ccggatgcaa cgcttaataa
    42001 ggcgtacatc cggcaatgaa actcaagcta aaccaataaa gaacccggca atggtcgcgc
    42061 tcatcaggtt ggaaagcgtc gccgccgcca gcgcccgtaa accaagctgg gcgatttccg
    42121 gcgcgcgttt tggcgaaata gccgaaaacg ctccaacgac aacaccgata gaaccaaagt
    42181 ttgcaaaccc acagagggcg aacgagataa tggcaatggt cttgacatcc agcgtgccgc
    42241 tcgtttgcag atagggcgag aaattgagat aagcaacaaa ctcattaatc gccagtttct
    42301 gcccaatcaa acttccggca agatttgcat cactccagtc cacccccata atccatgcca
    42361 acggggccag cacatagccg aaaatgcttt ccagagagac attggcaaaa ccaaaccagc
    42421 cgccaactcc accaataatg ccgttgatca gcgcaatgat cgctacaaat gccattacca
    42481 ccgtcgccac accggcggcg attttcagcc cggtcattgc accgttcgcc gcagcttcaa
    42541 taatgctttt tggcggtgtt tcgctaaacg acagattttc aaacgtaact tgcgaaggtt
    42601 ccgtcgccgg actaagaata cgtgcaaaca ggataccgcc aggaatagcc atcagcgaag
    42661 cggctaacaa atagtcaatc ggcacgccca ttccggcata gccaatcatc atcgaacccg
    42721 caatagacgc catgccgcta caaatagccg taaatagctc gttgcgattc atgcgatcaa
    42781 taaaaggttt aacgatcgcc gggatctcat tttgcccgag gaaaatagtg gtgactgcga
    42841 caaaagattc gattttgctg atgttgaggg ctttctgaaa aatactgcca agaatacgaa
    42901 tcagcagccc cattacgcca atgtagtaca gcaagctgat aagcgcggta acgaaaataa
    42961 tcgccgggag tacgcgaaag gcgaagataa agcccgcacc gtcaaacaga acatccattt
    43021 tgggaccgac cagcgaacca aaaataaagg cacttcctgc gtcactgtag gacatcactt
    43081 tatggacgcc taatgccgcc tgctctactg cccattttcc cgggggaaaa tagagcatga
    43141 tgccaccaat ggcaatttgc agcagcagcg cagcgccaac cgtgcgcaag ctgatgctct
    43201 ttttatttac cgacaacaca aaggcaattg ccagcaatac caccatcccc gcaacacttc
    43261 tcattatatc cataatgatt ttcccttcat gccagtaaac ccggcgtcag cgccaggttt
    43321 tggtatgttt gatgagtacg ggcgacggct ttctgcccgc cagaaattaa cccacgagac
    43381 gctgatattc tttggcaatt tcgctcgcca gaatggcgtt attaaagacc agttgaatat
    43441 tggatttcag gctatcaccg ccggttagct ccgcaacacg cgccagcagg aacggtgtac
    43501 tctctttccc aataacaccc tgttcttcgg cttcagcaac ggcttgatca attgctgcat
    43561 tgatggtttc ttctggcatc gcaaactgtt ccgggatcgg gttcgctacc accaggccgc
    43621 cgttcaaacc ggtttgccac ttcactgcca tcgcgcgagc aatttcgcgg gcactgtcaa
    43681 gacgaatgct gacttcaaat gggctggttc ggcagaaaaa cgcaggcagc gcttttgtct
    43741 gataaccaat taacggtaca ccgaaggttt ctaaatattc tgttgttaat cctaaatcga
    43801 gaatagattt tgcccccgca caaacaacag tgacattggt atttgccagt tcctgtaaat
    43861 cggcagaaat atcgaaggta tgttccgctc cgcgatgcac accaccaata cctccggttg
    43921 caaaaacttt aatcccagcc agtgctgcaa taatcatggt tgatgcaacg gtagtcgcgc
    43981 catttttccc tgcagcaacg acaaaaggta aatcacgacg actaactttc gtcacgttat
    44041 gtccttcacg gcccagcaat tcgatttctt ctttacttaa gcccactttc atcacaccgc
    44101 caataatggc gatagtggct ggtactgcgc cctgtttacg aattgtttct tctacttcaa
    44161 ttgcggtttg ggcattttgt gggaatggca ttccgtgaga aattatggtc gattccagcg
    44221 caacaaccgg ttttttattt tttaaagcat cctgtacttc agcggatatt tgtaataatt
    44281 caggggacag agttaattca gacattctgt gttctccact aacgatctaa cattggcaat
    44341 tgataattca gggttgttgg tatattcaca ggcaagtgcc attgacgaac atccctgcgc
    44401 aaatcgaaca gaatcgataa acggcatacc gtctacccaa caggaggcca gccccgccat
    44461 catggcgtcg cctgccccag taacattgat gacattcgtt ttaatagggg ccgaccaccc
    44521 actttcaccg ttgatatcac tgtagtaaac gccatcccct cccatgctca gcaccaagcg
    44581 gttcaggccg tgttgatgaa accaggcggc gacctttgcg acatcttcac gtccggaaag
    44641 tgcaattccg ctcaacgttt cggcttcaag acggttaggt tttaacgtat ggattttgct
    44701 cagatgatcg cggattttga cgcatttcca tgccgatacc ggatcaataa atacaggcgt
    44761 ttccccgcta ttttccagaa cccagaccag cgcctcttca ctaaggttgc aatccgcgac
    44821 aataaccttt gcaccgcgaa taaactcctg atgctgggca agaaattctg cagaaataca
    44881 gtcactaata ctcatatcat ttatcgcgac aagcatttct cctgtattat cgagcagtga
    44941 taaatagctg gatgtattct cacccggaac aattaagcat ttatcgacat aaacaccgga
    45001 ttgattagtt tgcgccagta atgattgccc ataaaaatca ccacctactg cacttaaaag
    45061 ccaggcattt ttccccagta acgccagatt atgagcgata ttccttccca cgccacctgg
    45121 ggtaaattta atttttccgg gattcgaatc cgcataattc aatggatgat gtgaataccc
    45181 agccacatca atattcgccg aaccaataat tactatgtag tccttttcgc gcatagcacg
    45241 ccccctggca ataaattgcc aacgaagaaa ttgggtaaaa tagccattac agtcaataag
    45301 actattagag cacgtgttca gatttaaaca catgttcata ataagcatat ttaacgtaat
    45361 gtaaatcgat cagaaaaaac gaaatcggcc agatatgaag gcggtcacaa agtttgtcta
    45421 tttatgcagc aacaaaagag aaagttgtga tgtgtaacac acaatatggc aggcaagaaa
    45481 aagggcagag atgtttctgc cctccagatt gttgacaacg cgcgctttgt tcatcctgga
    45541 tgtggcgtaa acgccttatc cgacctacaa aagcgcaaaa aattcaatat attgcagggt
    45601 tcatgtaggt ctgataagca tagcgcatca ggcaattttg cgtttgtcat catcggggca
    45661 gagatgtctc atccccgata atgaagataa attatgcgtt tttttctact atttgtgttt
    45721 ccgggcgttt caggaaggca taggccaaac ccgccaccag cgtaccggca ataattgcta
    45781 ccaggtaacc caataccggc gtaatagcgc cagggatcag cagaacaaac agaccaccgt
    45841 gtggtgccat cagtttcgca ccaatcgcca ttgagattgc gccagtcagc gccccaccaa
    45901 cgatacagca cggtagcaca cgcatcggat cacgagcagc aaacggaatt gcaccttccg
    45961 aaataaagca cagaccaagt accagagcgg ctttgccacc ttcctgctgc gctttgtcga
    46021 atttgcgacg cgccaccatt gttgccagac ccattgccag cggtggcacc atacctgccg
    46081 ccataatcgc cgccatcggg ccataggttt gagtactcag cagacccaca ccgaatgcgt
    46141 acgctgcttt gtttaccgga ccgcccatgt cagtacacat catgccaccg aggatcgccc
    46201 ccagcagaac cgcattcgca gtccccatgg tctgcagcca gtgagtcaac ccatcgagaa
    46261 tgccagcaac cggtttaccg attaggtaga tcatcgccag accgaccacc agactggaaa
    46321 ttagcggaat gatcaggatc ggtttcagcg cctccatact ctgtggcagt ttcaattgcg
    46381 tactgattaa cttcgcaatg taaccagcca ggaagccagc aattataccg ccaataaagc
    46441 cagaaccggt gctgaccgcc agcataccgc caatcagacc cggtgtgagg cccggacgat
    46501 cggcaatgga gaaggcaata taacctgcca gtaccggcac catcagcgca aaggctgaac
    46561 caccaccaat ctgcatcagc gccgctgcca acgtacccgg ctctttaaac gcttcgatac
    46621 caaaagcaaa agaaagcgcg atacacagac cacctgcaac aaccatcggc agcatgtaag
    46681 agacgcccgt cagcaagtga cggtaagcgc ctgcactctc tttcttacct tcagtggtcg
    46741 ctgtttgagc tttgcccgct ggttcatacg gcgttgcttc agcaaccgct ttatccagtt
    46801 cctgcgcagt tttcttcagc gccagaccgg tagaggtgcg atacatcggt ttaccagcaa
    46861 atttcgccag atccacttcg atatctgccg ccacaatcac cagatccgct gctgcgactt
    46921 cttcaggggt gattgcattg cccgcgccaa cagaaccacg ggtttcaact ttcacccacc
    46981 agccacgttt tttcgcttcg gtttcaatgg cttcagccgc cataaaggtg tgtgctacgc
    47041 cagtcgggca agcagtcacc gcaactacgc gtttcggacc gctggcagca accggtgctg
    47101 tcgcagcgac cggcgcagtg taaggtttcg catggccttt ggcttcactc aggaacagct
    47161 cagggtgcgc aactgcccgg gaaatatcac ccagccagac atttttaccg ttcagcgcgc
    47221 tgtcattcgg gatggaatca ccgagaacaa tcgccatttc tgcgtcgttc ggattgtcga
    47281 tgatttccag ttttgctttt cgcgccgccg cgcccagcag ggtcttcgcc atataggcgc
    47341 gtgcctgacc gagattagcg tcaataatca gcagcgtttt cattatgcct ctcctgctgt
    47401 cagttaaaag gttgtaagtc gacgcgcgcc atcattgcgg ccaactgcgg acgatcggta
    47461 atacccacat tgctttgact taccgccagg gctgcaacag ctgtcgccag acgcagtgtg
    47521 tgttcactgg attcacgcat cagcaagcca taaatcaggc caccaaccat agaatcccct
    47581 gcgccaacgg tgcttacgac atcgactgac ggtggtttgg cgatccattc gccggaggca
    47641 ttaacccaaa gcgcgccttc ggcacccagt gaaataacaa catgcgcaat accttgttca
    47701 cgtagcgcat gtgcagcttc aatcacatct ttcatttcag gcagtttacg gcctgcccag
    47761 atttccaact cgcggcggtt aggtttcacc agccacggtg ccgctttcaa acctgctact
    47821 aacgcttcac ggctactatc aaagataatg caaggacact gactacgcag gcgagtcatc
    47881 cagtcggtga acgcttccgg gctgacgcct gacggtaagc ttccgctgac acagaccata
    47941 tcgaactgac cgagccagct cagagaatca gtcacaaagc gttcccagtc ggcgagggtg
    48001 acttcaaaac ccgagaagtt gaagtcggtc acttcgccgt ctttttccgt cagcttaacg
    48061 ttaattcggg tgcgcccctg tacaacctgg aaacggttgg caatgcccag ctcgctgaac
    48121 agttgctgaa aaccatcctg attgtcttta cccaggaagc cgccaacggt gacatcaatt
    48181 cccaggtctt ttaatacttt ggccacgttg atgcctttac ccgccgcatg cagaccggtg
    48241 gttttcacca ggttcacttc gccgcgttca atttccgggc agaaaccaac aaggtcataa
    48301 gccggattaa gggtgatcgt agcaacacgt ctgctcatta tgcgccctcc ccaagaccag
    48361 cagcgatagc gtcgccaatg gctttcagcg cctgttcagc atctgcaccc tgggcggtaa
    48421 agcgtaggcg atgacctttc ttaacgccaa gtgccacaac tttcatcaga ctacgtccgt
    48481 ttgccggttt gccggtgcca tcaaggtttg tcacggtaat atcactgtta aattgtttaa
    48541 tggtattgac cagcatggta cctggacgag catgcaggcc gtgttcattg cgcaccacaa
    48601 actccgcgct taacacgtcg tcggtcggcg catcatcgct ggtcagcagc gccagcaacg
    48661 ttgccgcatc cgctttcagc aagcggtcag ctttattgtc gagcaataaa tcagcgagac
    48721 gcttaagaac cgcgatgggc tgatcgtcat tcatcgccac actcaccagc atggctgccg
    48781 tttcgccgtc cacatcaaaa gcatttgccg cacggcttac cgcaatcgcg ctacgcagat
    48841 tgccttcggc gctatcgctc agccagatac cctgtccgag attcagcggt tgttcattga
    48901 tggctttggt gacgaaagtg gcgtcaactg cccccacctc tttcagacgc gcagcgttca
    48961 gcgcctgaag agtcagcaga tcgctggcga cgatatccag cgtcagcatt tcgttgtcga
    49021 gcttcagctg ctcactctgc ttttcgccca tcagtaatgc gcgaagttct tctgctgttg
    49081 ttgctgactt cagttgttca gcaacggaat catcgctcag tacgtgggtc agctggcgta
    49141 gcaggcccag atgttcatcc gagctggcag caataccgat tgccacgtac gctacctgac
    49201 cgtcacccca ggtgacgcct tccgggaact gaaatacctg aacgccggtt ttcagcacct
    49261 gatcgcgggt gtcggtagtg ccgtgtggaa tagcaatacc attgccgagg aacgttgagg
    49321 tttgctgttc gcgcgccagc atgccattga cgtagccttc tgctacatta ccggcctgca
    49381 ccagcgccgc agcgacctgg cgaatcgcct cttctttgtc tccggccttt tcgcccggat
    49441 ggatgtcctg tacggataac tggaacatag ttctcctctc ttgctgaatt gaaacgattc
    49501 agcctctatg agaaaaaaag cgccaacctg gcttagggtt aaagacaaga tcgcgctgaa
    49561 acgtttcaag aaagcataat acttctgttt cagcacgcaa ggaaagccgt aaagttatga
    49621 gcaaaagttt gatgtgctgc acaatttttt gtcatttttc ctaattgctg atgggaaaat
    49681 ccgttatcag caatttcatt tcagcagcgt cagcagccaa tcagcaacag tgaaatggca
    49741 ttttgattat tcgtggcgct tttgatttcg ctgtctgttt attttctgac aagcagcgta
    49801 aactccgcgt cttcctcttc cagtgatcga cctgcatgca taactccccc gcagtctcca
    49861 gcgcgaaatc gtttgacctg acctcgacgg cgtttttaat cgttgccttt ctcaccggta
    49921 ttgcgggtgc tctgcaaacc ccgacactca gtattttcct taccgatgaa gtacatgccc
    49981 gtccggcgat ggtgggattc ttctttaccg gcagcgctgt cattgggatt ctggtaagtc
    50041 agtttctcgc tgggcgctct gataagcgcg gcgatcgcaa atcgctgatt gtcttttgct
    50101 gcctgttagg cgtgctggcc tgcacccttt ttgcctggaa tcgcaactac tttgttttgc
    50161 tattcgttgg cgtctttctt agcagctttg gctcgaccgc taacccgcaa atgtttgccc
    50221 ttgcccgtga acatgccgac aaaaccggac gtgaggcggt gatgttcagc tcttttttac
    50281 gcgctcaggt ttcactggca tgggtcattg gcccaccgct ggcttatgcg ttagcgatgg
    50341 gtttcagctt tacggtaatg tatctgagcg cagcggtagc gtttattgtt tgcggcgtga
    50401 tggtgtggct gtttttaccg tcgatgcaaa aagagcttcc gctggcgacc ggcacggttg
    50461 aagcgccgcg ccgtaaccgt cgcgatacgc tactgctgtt tgtcatttgt acattgatgt
    50521 ggggctcgaa cagcctgtac atcatcaaca tgccgctatt tattatcaac gaactgcatc
    50581 ttcccgagaa actggccgat gtgatgatgg ggaccgccgc cgggctggaa atcccgacga
    50641 tgttgattgc cggatatttc gccaaacgtc tgggtaagcg tttcttaatg cgcgttgctg
    50701 ccgtgggtgg cgtctgtttt tacgcaggaa tgctgatggc gcattcacct gtcattctgt
    50761 tgggcttgca gctgctaaat gctattttta ttggcattct gggcggcatc gggatgctct
    50821 attttcagga tctgatgccc ggtcaggcgg gttcagccac cacgctctat accaacactt
    50881 cgcgcgtggg ctggatcatc gcaggatcag tggcgggcat cgtcgccgaa atctggaatt
    50941 atcacgctgt gttctggttt gcgatggtga tgattatcgc cactctgttt tgcttactgc
    51001 ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag gtcatggcct
    51061 gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag actttcggtc
    51121 gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg cagggcgtat
    51181 tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg gcagtgcaac
    51241 acgccccgca acccggacgg cattccatac gttgttctct taaggttaac caccggattg
    51301 atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc acttgcccga
    51361 aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac aggaattttt
    51421 cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac ggcaaactgc
    51481 tgctggttaa ggatattgat attcagtcgc ccactgcccg cggtgccgct acgctgtaca
    51541 aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc aaaggtgatg
    51601 atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat gtcgatggca
    51661 acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc aaagatcaga
    51721 ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag gtgctgacct
    51781 gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa atcgttgaaa
    51841 ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg acattgagca
    51901 ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt cgtatccata
    51961 tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca gaaaagggat
    52021 agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg cttcctgaac
    52081 accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat cgcattaaca
    52141 atccagacca atttcaattg ctgtcatata actttacact gtcattgtta atttatcgtt
    52201 actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat catgtacacg
    52261 ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc tttggcgcct
    52321 ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag ggcggcgact
    52381 gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag ctccgcgcac
    52441 agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg ataattgtcg
    52501 gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt attgagaaat
    52561 tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc tattgtattg
    52621 acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat ctacaaaccc
    52681 ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc cgccgtgagc
    52741 gcggccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat ggccatgtgg
    52801 tgaaaaacgc ggtgctggga atggcagaaa aatgttcgcc agaactcgcc gggtggataa
    52861 aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct gcaaccgacg
    52921 aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg attagctgcg
    52981 aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct gcctgggaag
    53041 tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa ctgcgtatgc
    53101 ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc gtccatatca
    53161 gtgattgcat gcaggatcgc gtatttcgcc atgctgccag aacattaatg ctggatgagc
    53221 aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat aagttaattg
    53281 cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg gatggcagcc
    53341 agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt gaaacggact
    53401 ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc gttgatgatg
    53461 ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa cttgttgcgg
    53521 gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt ttcggtgatg
    53581 atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa caaatcgtac
    53641 aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt ctgcggttaa
    53701 agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt tttagccagg
    53761 attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg accaacctca
    53821 tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta gcccataaag
    53881 atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga attgatggtg
    53941 ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc atgtgctgcg
    54001 ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga aaaccagacc
    54061 gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat ctgttaaccg
    54121 caccagtcta tgaactgtgg atagatctgc gcgccacctt gtgcattctc gatccacgcc
    54181 tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg gctgccgcag
    54241 acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa gcgctacagc
    54301 gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat gggaaagttg
    54361 acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc agcgccgcgc
    54421 attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca gagcatcaac
    54481 gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg atattcgacg
    54541 ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca ccggtggaac
    54601 gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt cagggcgatg
    54661 acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag ctgatttcca
    54721 gcagcgaagc tgactggaat gctttgcaga gcgcgctgtt gaagcttcgt ttagcgacta
    54781 ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga ttaaaaattt
    54841 gccgcaaata gtgttgttga atattgtcgg cctcgcgctg tttctttcct ggtatattcc
    54901 cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct ttaatcagaa
    54961 actggtcgaa agtaaggcct ttttgtggct ggttgcattg accaacaatc gcgctttcga
    55021 cggttgttct ctgctggcga tgggaatgtt gatgctgagt ttctggctga aagaaaacgc
    55081 ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg cagtggtatt
    55141 aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat tgacttttac
    55201 cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct cacgagatag
    55261 ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt ggcgttattt
    55321 cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat ttcccagagt
    55381 aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg tgatattgat
    55441 cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct tttttgacaa
    55501 atcactaccg ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa ttaacatcat
    55561 caggcttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt catccatcgc
    55621 gcgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc aatcgtactt
    55681 taattgacca atcccgctta ttaactttct gtatcacttt ttcttataaa aaatcatgta
    55741 aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc ctcggcggta
    55801 gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct taaggaattg
    55861 tttcaacatg cccaggtaat tagtctcgtg ccgcttggca tttttttata acgatatttg
    55921 tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga ttttgagata
    55981 tatcttgcgc gggatccccg cgattgcagt agcggttctg ctttctgcat gtagtgcaaa
    56041 taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa catcatcact
    56101 gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa aatcgcgaat
    56161 tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca gcactaaaaa
    56221 aggtatcgat tgttctggtt ttgtacaacg tacattccgt gagcaatttg gcttagaact
    56281 tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca gtaatttgcg
    56341 tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg gtatttatat
    56401 cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt ccagcatgaa
    56461 tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc gcagctaata
    56521 aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca ctgcttaggc
    56581 agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg atccaggcta
    56641 tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta acggaatcat
    56701 gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg tagcaggcgt
    56761 gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata agcacgaagt
    56821 caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt ttgccgacct
    56881 gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc aggctaaccc
    56941 cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc tggtgaaaga
    57001 taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca atgaattgat
    57061 tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca cgccgatggt
    57121 gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa atagcggtgt
    57181 gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac ggcaggaaga
    57241 ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt cttcacgttt
    57301 gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg cgggcattcc
    57361 tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt acgcattttt
    57421 actgggcggc atgagtggaa ctgtcgttgg cctgctctgc tattacctga tgagcgtacg
    57481 tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt acgtggcgta
    57541 tcaaccggtg gtggatacac aagctttgcg agtaacgggc ctggaagtac tgctacgctg
    57601 gcggcatcct gtcgcgggag aaattccccc ggatgccttc attaactttg ccgaatcgca
    57661 aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg atgccgcaga
    57721 attagaaaaa gtgctgccag taggcgtcaa atttggtatt aacattgcgc cggaccatct
    57781 gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc ccgcacacca
    57841 tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag aagccacaca
    57901 actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact tcggcaccgg
    57961 gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa ttgaccgtgg
    58021 atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg cggtgctgac
    58081 gctggcgaaa cgcctcaata tgctgacggt tgctgaaggg gtcgaaacgc cggaacaggc
    58141 gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta gtcgcccttt
    58201 accgctggac gattttgtcc gctggctgaa gaaaccgtat acgccgcagt ggtaaggtgt
    58261 gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat gatgagtaaa
    58321 aggaaatcca ttgcagatga ttgtgcgcat actgctgctg tttatcgctc tgttcacctt
    58381 tggtgtgcag gcgcaggcta tcaaggaaag ctatgccttt gccgtgctgg gcgaaccccg
    58441 gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa aaggtgggca
    58501 gataacgttg tcagccctcg gcaccttcga taatttcaac cgctatgcac tgcgcggcaa
    58561 cccgggcgca cgcaccgagc agctgtacga cacgctattt acgacttccg atgacgaacc
    58621 aggcagttat tacccgctga ttgctgaaag cgcacgctat gctgacgatt attcctgggt
    58681 ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tcgcccatta ctgcccgcga
    58741 tgtagagttt acttttcaaa aatttatgac cgaaggcgtg ccgcaatttc gtctggtcta
    58801 caaaggcacc accgtcaaag ccattgcacc gttaaccgtg cgcattgagt tagctaaacc
    58861 cagcaaagaa gatatgctga gtctgttttc gctgccgata tttccagaaa agtactggaa
    58921 ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc cgtaccgcgt
    58981 tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt actgggcagc
    59041 aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg attattacct
    59101 cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc gtatggaaaa
    59161 cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa aatacatcat
    59221 caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt ttaatatcca
    59281 acgtccggta ttcagcgatc gccgggtccg ggaagcaatc actctcgcct ttgactttga
    59341 atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt attttcagaa
    59401 taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc tggcaccaat
    59461 gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg tatctaaagg
    59521 cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg aaacgggctg
    59581 ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca gctttgaatt
    59641 attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca gcctgcaacg
    59701 gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta accgcatgcg
    59761 cagtcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc ccagttccga
    59821 tttacagatt tcctggtcat cggaatatat caattccact tataatgccc ccggcgtgca
    59881 aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa ataaagaaaa
    59941 attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt acatgctgcc
    60001 aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc aacccgctgt
    60061 acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata aagcggccaa
    60121 actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc gccgtctgtt
    60181 gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg tgcaaattgc
    60241 gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg ccggagtatt
    60301 acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg tcggcaatat
    60361 cagcgacagt aattaccgtg gcggacgcgg attagaccca gaagtgatcg ctgagatcac
    60421 tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc tctgggacta
    60481 catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga cgctgattaa
    60541 agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta tctatctggt
    60601 gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg acgtctggag
    60661 tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca tcctgctgat
    60721 tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc tggtttccgc
    60781 taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc atatcacgct
    60841 accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga ccaaaaactc
    60901 attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag gggtaagtga
    60961 gaaaaacatt ctctggaaac atgtgttccg caacgccatg ctgctggtga ttgccggttt
    61021 tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg tgatgttttc
    61081 actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt atcctgtaat
    61141 gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg tcagtgatat
    61201 cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc gcgactcagc
    61261 cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta ctggtcgtta
    61321 tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc taacgataaa
    61381 ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa ctacagcgaa
    61441 agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg gctgaaacaa
    61501 cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc taccagtatc
    61561 aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg gctgggaacg
    61621 gatgccaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat ctcggttctg
    61681 tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg ggcgctacaa
    61741 ggctattacg gcggtaaagt cgatctctgg ggacaacgct ttattgaagt atggtcgggg
    61801 atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa cttctggtgg
    61861 ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt gcgggcggag
    61921 tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg cgtcagcgat
    61981 cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct caccttttta
    62041 ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct cggcttcggt
    62101 ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa taaccttcag
    62161 gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc tttgctgatc
    62221 tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta gcatgacgca
    62281 aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa ccgtacgtac
    62341 agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc tggtgggtga
    62401 gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc cttccccgcc
    62461 ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc atgccagcga
    62521 tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg aaccgatggt
    62581 gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt cactccaccg
    62641 cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc gcgtcggtat
    62701 ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg gcgaacgcca
    62761 gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg ccgatgaacc
    62821 gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc gcgaactgca
    62881 aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg tcagaaaact
    62941 ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata acgccgctac
    63001 gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg aaccgtcagg
    63061 cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac agcttcaggt
    63121 tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg tggtggtgaa
    63181 aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg agtctggttc
    63241 cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg gcagcatcgt
    63301 ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta ctcgtcatcg
    63361 tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca acgttttgca
    63421 gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac aacgcgaaca
    63481 acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc accgttatcc
    63541 ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat taattcttaa
    63601 gccctcactg atcatacttg atgaaccgac atcatcactc gacaaaacgg tacaggcgca
    63661 aatattgacg ctattgaaat cattgcaaca aaagcatcaa ctggcctatt tgtttatcag
    63721 ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc gacaagggga
    63781 agtagtggaa caaggaccgt gcgcgcgcgt gtttgccgca ccgcagcagg agtatacgcg
    63841 tcagctactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag ggttcggcaa
    63901 ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg cgatttacaa
    63961 acaggcacgg ctcaccttca cactccagca ccgaacatgg aacttcgatg tcgcttaacg
    64021 cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta agcaatttaa
    64081 gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca accttcgaac
    64141 ctgcgaaacc agcagaccag cgaaagttct gcggtagacg atggacgatt gagagttgta
    64201 atgaagtcac ttattttccc cggaagcaca tttacttcac aatttattta catcaatttt
    64261 aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct cgcctgcatg
    64321 gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc ggggctatat
    64381 gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac tttttacata
    64441 aattgatttt acataaaata aacatatatc gggggaatga tagatttgtg gttgacatgc
    64501 atcaacaaag gaagcctttt agcttcctcg ttgtgcaata gatcaccgtt ttttcggtcg
    64561 actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg accaaatcat
    64621 cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg cccctatgcc
    64681 aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa actcatcaag
    64741 aatgaccgcc atcgcattgg atgacaccat agacacgcag cccacaaacg ccgcaacgcc
    64801 aaccaccagc gaccagaatc ccagccccag cagcgcactg atgaccatcc acgctgccat
    64861 aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc ggcggacgaa
    64921 gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa agtaataacc
    64981 aaagttttcc ggtgcaacgt ggttgatttc aatgtagaca aacggtccgg cgctcaggaa
    65041 cgagaacatt ccggcaaagc tgaaaccgct ggccagcata taactcagga cacgcttatg
    65101 acggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaaaggct ggcgaggatc
    65161 cagaagatgt aatgccagct cagccacacc agcacccagc cgccaactat cggtgccatc
    65221 agcggtgcaa tggtcgtcac cagcatgaca aacgacatca tccgcgagaa ctcttctttc
    65281 gggtaaatat cacgcatcaa agcgttgata accacgctgg ccgcagccgc agccagcccg
    65341 tggaagaaac gcatcacaat cagctgatcg atggtttgtg ccaacgcaca cgccaccgcg
    65401 gcggcggcaa acaccagcgt accgccaaga accaccggct tacgcccgaa gctgtctgcc
    65461 atcggcccgt agattaactg ccccaacgca aagcccagaa tataagtact cagggtcatc
    65521 tgcgtactgc ccgccggtac gccaaactgc gctgaaatta ccggtagcgc gggcagatac
    65581 atatcaatcg acagcggcat caacatggcc agcaggccaa ggataaaaac aatagcaaac
    65641 gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt cattgaaaag ttagacgacg
    65701 ctggcaattt cttcttcagt taacggacga tattcaccgg gggctaaatc agcatccagc
    65761 gtaatgccgc caatacgttc acgatgcagc tcaaccacat ggttacccac ggcggcgaac
    65821 atgcgtttca cctgatgata acgcccttcg ctgatggtca gacgaacctg cgttggggta
    65881 atcacttcca gcaccgccgg cttagtgaga tctttttcgt tatgcagctg cacgccttta
    65941 gcaaattgtt ctgccgtatc gtcagctacg ggtgatacca gcgtcaccag ataggtcttc
    66001 tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact gaccatcatc tgtcattagt
    66061 accagaccgg tggtatcgat atccagccgc cctgcggcgt gcagtttcca cgctaccggt
    66121 tcatctagaa aatagagcac cgttgggtga tcagggtcgt ccgtggagca aacataaccc
    66181 tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct gcgccagcgg gttgccatcg
    66241 taagcgacat catgttcagg aagcagtttg aacgctgcat tacggacgat ttcgccatcg
    66301 acggtgacac gattgccgcg gatttcacgc ccggcaatag cacggctaac gccgagttgc
    66361 tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc ctgtaaaaaa cggaggtcgg
    66421 gtaatgaccc gaatatctga actgttgtct gcccagtata gcggtctaat aacatccctc
    66481 aagggaaaaa gatccgtggc atactattag cagaataatc tacctacgcg agaccatgat
    66541 ttttacactt cgcccatatc agcaagaagc cgtggatgcc acgctcaacc attttcgtcg
    66601 tcataaaacc cctgccgtta tcgtgctgcc caccggtgca ggtaaaagcc tggtgatagc
    66661 ggaactggca cggctggctc gtggtcgcgt actggtgctg gcacacgtta aagaactggt
    66721 ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa gccgatattt ttgccgccgg
    66781 gctgaagcgt aaagagagcc acggtaaagt ggtatttggc agtgtgcagt cggtcgcccg
    66841 taatattgat gcctttcagg gtgaattttc gctgttgatt gtcgatgaat gtcaccgtat
    66901 tggtgacgat gaagagagcc agtatcagca aatcctcact cacctgacaa aagtgaatcc
    66961 ccacttacgc ctgctggggc tgactgccac gccttttcga ttgggcaaag gttggatcta
    67021 ccagttccat tatcacggca tggtacgcag cgatgagaaa gcccttttcc gtgactgcat
    67081 ttatgagctg cccctgcgtt atatgattaa acacagctat ctgacgccgc cagaacgact
    67141 ggatatgcca gtagtgcaat atgatttcag ccgcttgcag gcacagagca acgggctgtt
    67201 cagcgaagcc gatctcaacc gtgagctgaa aaaacagcaa cgtattaccc cgcacatcat
    67261 cagccagatt atggagttcg ccgaaaagcg taaaggagtg atgattttcg ccgcgacggt
    67321 cgaacacgca aaagagattg tgggattact gcctgccgaa gatgcagcac tgattactgg
    67381 cgacaccccc ggcgctgagc gcgatgtgtt aattgaaaat tttaaagccc agcgttttcg
    67441 ctatctggtc aacgtcgcgg tactgaccac cggatttgac gccccgcacg tcgatcttat
    67501 cgccattctg cgccctaccg aatcagtgag tctttaccaa caaattgtcg ggcgcggtct
    67561 gcgtctcgct ccgggcaaga ctgattgttt aattcttgat tatgcgggta atcctcacga
    67621 tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt gacaacgttc cggtacaggt
    67681 tttctgccct gcctgcggtt ttgccaacac cttttggggg aaaacgaccg ccgacgggac
    67741 attgattgaa cactttggtc gccgctgtca gggatggttt gaagatgacg acggtcatcg
    67801 cgaacagtgt gacttccgtt tccgttttaa aaattgcccg caatgtaacg cagaaaatga
    67861 tattgccgct cgccgctgcc gcgaatgtga cacggtactg gttgatccgg atgatatgtt
    67921 aaaagcggcg ctacgactga aagatgcgct ggtattacgc tgtagcggca tgtctttgca
    67981 gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc tattacgatg aagacggtgc
    68041 ggatgtgagt gagcgtttcc gtctgcaaac acctgcccag cgtaccgcct tcgagcagct
    68101 ttttatccgc ccgcatacgc gcacaccggg catcccgctg cgctggatca ccgccgccga
    68161 tatcctcgcc cagcaagcct tattgcgacc cccggatttt gtcgtcgccc gcatgaaagg
    68221 ccagtactgg caggtgcgtg aaaaagtgtt cgattacgaa ggtcgttttc gtcgggcgca
    68281 cgaattacgc ggttaatggt agttttgatt gatgtataag gcgattgagt atagaatctc
    68341 acccgctttt gcatacgcaa agcagatcac ttacctgttg ctgggtcgcc tgtagcagga
    68401 ttaatttaag agagaaagaa atgtttacta tcaacgcaga agtacgtaaa gagcagggta
    68461 agggtgcgag ccgccgcctg cgtgccgcta acaagttccc ggcaatcatc tacggtggca
    68521 aagaagcgcc gctggctatc gagctggatc acgacaaagt catgaacatg caagctaaag
    68581 ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg taaagaaatc aaagttaaag
    68641 ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca gcacatcgac ttcgttcgcg
    68701 cttaattgct gaataagttg taaaaaaccc cgctccggcg gggttttttg tatctgcaga
    68761 ttatgcctga ttacggtatt gctatttttt gcaggccaga taaggcgttc acgccgcatc
    68821 tggcattcag aacacaaaac cgattaattg ccgccagacg tccggcgctg caattggtcg
    68881 cgcaaattcg gcggcgtgcc tttaatggtc agggtatcgg tcgccggctc ccagaagatg
    68941 cgttcaccta acagcatcgc atcaaagtta atagtcaagc caccgccgct accggcaaac
    69001 ttcgtcaact gacgcagtgt gctccggtct gccgggaagc tctcttccag ttcgtagcct
    69061 ttttcagccg caaactccgt gaagctcact tcgcttacgc ctgccagctc tttcgacagc
    69121 gattccagtt cgatctcttc gcctgcctgc agctgctcat tacagtagct ataaacctgc
    69181 tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt cggcagtgaa atcatcaacc
    69241 gcttgcaaca gcccacggtt ttgcgctttc gtgttcaatc cttcgctggc cccgaggaaa
    69301 tccataaaga aatccgccac tttgcgccct actcgccctt tcaggaaagt gagatagcgg
    69361 gtggactctg gattggtttc ccattcggtt aaatcgatac gcgcaacaat atccgcatga
    69421 ttgatatcaa gataatgggt tgggttgatg tcgagatttt cgttaacacg catactgctc
    69481 aggttgctca gaactgccac cagcagatac tccaccgcca gataacggta gtggcaaaac
    69541 agcacgaatc caccgtcagc aaaaggatat ttcgctaatt cgtcacgcag gcgaccagtt
    69601 gccgcgcggc taaatgccag aaagtcctct tcgccctgac gctgtaagcg cagcgtctgc
    69661 gccagttcac tctcttcgct aaacaagccg taagctttat ttttggcgct atagacccga
    69721 tgcagttctg ccaccatctc aacgacggtt tctgtcggtt ccagcaatga atcgcgcaac
    69781 accagctcaa ggttctgctc atcacgcttg ataagctggt gcagagcaat ctggttgata
    69841 tccagactca tgataaactc tcctttaaga ccgggcggta ttcaaccacc gccggttaag
    69901 cgacgcaata aaagatggcg gtcattttac agaaggcagc gtaacgagaa tgatttaacg
    69961 cataaaaaag cagaaaaaaa accgttgcta cggtaatatg ttgccctttc atcaactaac
    70021 tgatttcgat ttatgccaca aatttcccgc tacagtgatg aacaggttga acaattgctc
    70081 gcagagctgc tcaacgtact ggaaaaacat aaggctccga ccgacctttc cctgatggtg
    70141 ttaggcaaca tggtcactaa ccttatcaac accagcattg ccccggccca acgccaggca
    70201 attgccaact cttttgcccg cgccttacag tcctctatca acgaagacaa agcgcactaa
    70261 gggaaacaga taacaggtta tggtaactca tcgtcagcgc taccgtgaaa aagtctccca
    70321 gatggtcagt tgggggcact ggtttgcact gttcaatatt ctgctttcgc tcgtcattgg
    70381 cagccgttac ctgtttatcg ccgactggcc gacaacgctt gctggtcgca tttattccta
    70441 cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc acctacttgc tgatcctctt
    70501 cccgctgacc tttatcgtcg gctcccagag gctgatgagg tttttgtccg tcattctggc
    70561 aacggcggga atgacgctat tactgatcga tagcgaagtc tttactcgtt tccatctcca
    70621 tcttaatccc atcgtctggc agttggttat caacccagac gaaaatgaga tggcgcgcga
    70681 ctggcagctg atgttcatca gcgtgccggt tattttattg cttgaactgg tgtttgcgac
    70741 gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga cgcttcgcgc gcccgctggc
    70801 cgcattctta tttatcgcct ttatcgcctc gcatgtggtg tatatctggg ccgatgccaa
    70861 cttctatcgc ccgatcacca tgcagcgcgc taacctgccg ctttcgtacc cgatgacggc
    70921 gcgacgtttt cttgagaagc atggtctgct tgatgcgcag gagtatcaac gccgtcttat
    70981 tgagcaaggt aatccagacg ccgtttccgt tcagtatccg ttaagcgaac tgcgctatcg
    71041 cgatatgggc accgggcaga atgtgctgtt gattactgtc gatggcctga actactcacg
    71101 cttcgagaag cagatgcctg cgctggcagg ttttgctgag caaaatattt cgttcacgcg
    71161 ccatatgagc tccggcaaca ctacagacaa cggcatcttt ggcctgttct atggtatctc
    71221 gccgagctat atggacggca ttctgtcgac ccgtacgcct gcggcattaa ttactgcgct
    71281 taatcagcaa ggctatcagc tggggttatt ctcatcagat ggctttacca gcccgctgta
    71341 tcgccaggca ttgttgtcag atttctcgat gccgagcgta cgcacccaat ccgacgagca
    71401 gaccgccacg cagtggatca actggctggg ccactacgca caagaagata accgctggtt
    71461 ctcgtgggtt tctttcaatg gcactaacat tgacgacagc aatcagcagg catttgcacg
    71521 gaaatatagc cgggcggcag gcaatgtcga tgaccagatc aaccgcgtgc tcaatgcact
    71581 gcgtgattct ggcaaactgg acaatacggt tgtgattatc actgccggtc ggggtattcc
    71641 gctgagcgaa gacgaagaaa cctttgactg gtcccacggt catctgcagg tgccattagt
    71701 gattcactgg ccaggcacgc cggcgcagcg tattaatgcg ctgactgatc ataccgatct
    71761 gatgacgacg ctgatgcaac gcctgctaca tgtcagcaca cctgccagcg aatattcgca
    71821 aggtcaggat ttgttcaacc ctcaacgccg tcattactgg gttactgcag cggataacga
    71881 tacgctggca attaccaccc cgaaaaagac gctggtgctg aacaataacg gtaaataccg
    71941 cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa ccacagttaa gtttgttatt
    72001 gcaagtgctg acagacgaga agcgttttat cgctaactga ttaattataa atcagttagc
    72061 gaaatatctt acttgcaatc ggtgtggaaa acggtagtat tagcagccac gagtcggcac
    72121 gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg gtcggaggtt caaatcctct
    72181 cgtgccgacc aaaaatccca agaaaaaacc aacctttacg gttggttttt ttatatctgc
    72241 aattaatttg ataaacagac cgtgacacat cacagcctgt ttattttctg ttatcagaac
    72301 gtccagacca ctcccgcctg agtattccac ggcgattcta cccctgcgcc attgccatag
    72361 ctgactgaca aatgaccgct taacgtcggg gtaaacgatg accttatccc agcctgataa
    72421 acgccacggg ttccagccac atcattatta aatttgccat cgtcattaac tttcacatgg
    72481 ttagaatcgg cctattcctg acgcacggcg gctttcagcc agggttccaa cgtcgtaccg
    72541 ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt cagcgcgtaa tatccgggta
    72601 tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt cctgaccatc tgtggtaaag
    72661 ccggtaaagg cgagataggg gctaacactc cacaatccgt caacccaacg gaacccgctc
    72721 tcaacatgag cacccgcgcc gttactattg taatcgccaa acgctgttgc cccattactc
    72781 atcttgccat ggatggtgtt ggcaaaacgg tcaactttca ccaccccatc aacataggca
    72841 ccgttctgat gctcccaacc ggcataagcc cccagggtgt agctatcaat attgcctttg
    72901 ccgccgcgat caaaaccaat atcagaatga gagtaatcaa agaacaagcc gcgaattgtg
    72961 ctgctttctt cacgggagaa acggctatcg ataccgagcg tcaggcccgt caatgtttgc
    73021 tcaaagccag ctcccgcatc agtggtcacg ttgttgcggg tgttaattgc cgaactccac
    73081 atcgccgtat cgtaattaac gccttttacg ctaccaagac gctcacgcac ggtgtccagt
    73141 tctgcatcaa ataccagcgg ttgtgctgct gccatgttca gcacatcagt ggttgaaggg
    73201 gtaatttgcg cgcgattctc tgcgaggctc cagctatggt tgccattatc cagcaaggta
    73261 tattcatacg taccgatatc aacaacgcct ccggcattgc ccaacgtgaa tgcagcatca
    73321 ccgccgcccg tcgttaccag tgtaaggcta tctcctgctg ccgggctggc ccccgtgtcc
    73381 gtcacgaata ttttgaaatc acctgttgcc tgaccgatga cgttgagctg atcgctctga
    73441 tgattagcca tatcggtacg catataaaat tgccgtttcc cgacagggta ttggtggtca
    73501 acgtaatgaa attttccgca cttgccgtca cagatgaacg agtcactggc tcagccatca
    73561 taatcacatt tgcgccggac atcgcaaggt tggctaagtt gaggcgagtg ttttggttcg
    73621 tcgtagtgat atcggcaatg atggtccatg cgctattttc gagcgaaacg tccgcttggc
    73681 tggtatccac ggcaccatgc gttctaaaag aagcgccctc agaaaccact accttaccca
    73741 ccaacatagc gggtgcataa tttattttcg gcttcatgac ctcaagaatg ccatcattcc
    73801 ctctgactga cacgttaacc attgtgccag cccagacatt agcgcggcca ttgttgataa
    73861 ccatattttc agagatggct ttagaggaat acgtgatact tccattctga tctgatcgac
    73921 cgagatcata tacagcattt gcctgcactt tcgtaccagt atcctttccc agcgattgca
    73981 tagtggcatg ctcatcaagg atagtgtcaa tggcctgagt gtcctccata acaatgagcc
    74041 cggagccatc atccagttca taattttttg acacaccatc ctttatacta aattggcctt
    74101 tactgtttgt accactcact tccagcgcat tcgttgagac aattaatttc ccgcctgctt
    74161 ttttatcaac gccagttgca gtcccgccat ccataacctc cagtaagccg ccactatcca
    74221 cagtggtatt ataagcgaag tcattttctt caactcgcaa acttccgccg ttttccaaca
    74281 gcatattgtt agcaatacca ttcttgattt cgaactgacc gagacggttt gttccgaata
    74341 cctccatgac ccgcgtggtt gttttaatag caccgcctgc tttctgatct accgcaaatg
    74401 ctaatccccc gtctttaacc tccagtaatc ctcccttttc gatagcggca aagacggcaa
    74461 tcccatcttt ttcaacaatt aagcgcccgc ctttaatcgt gggatcattc gccgcg
//
LOCUS       DAQSWQ010000006        72510 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.006, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000006 DAQSWQ010000000
VERSION     DAQSWQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.006"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="R8G18_000396"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="R8G18_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEE9794195.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="R8G18_000397"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="R8G18_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEE9794196.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="R8G18_000398"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="R8G18_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEE9794197.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="R8G18_000399"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="R8G18_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEE9794198.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="R8G18_000400"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="R8G18_000400"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HEE9794199.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="R8G18_000401"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="R8G18_000401"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HEE9794200.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="R8G18_000402"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="R8G18_000402"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HEE9794201.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="R8G18_000403"
     CDS             complement(4604..5326)
                     /locus_tag="R8G18_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEE9794202.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="R8G18_000404"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="R8G18_000404"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEE9794203.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="R8G18_000405"
     CDS             6526..6744
                     /locus_tag="R8G18_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEE9794204.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="R8G18_000406"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="R8G18_000406"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEE9794205.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="R8G18_000407"
     CDS             complement(7478..7678)
                     /locus_tag="R8G18_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HEE9794206.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="R8G18_000408"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="R8G18_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HEE9794207.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="R8G18_000409"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="R8G18_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HEE9794208.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="R8G18_000410"
     CDS             10175..12088
                     /locus_tag="R8G18_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEE9794209.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="R8G18_000411"
     CDS             12088..13110
                     /locus_tag="R8G18_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEE9794210.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="R8G18_000412"
     CDS             13104..13322
                     /locus_tag="R8G18_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HEE9794211.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="R8G18_000413"
     CDS             13376..14245
                     /locus_tag="R8G18_000413"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEE9794212.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="R8G18_000414"
     CDS             complement(14300..14704)
                     /locus_tag="R8G18_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEE9794213.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="R8G18_000415"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="R8G18_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HEE9794214.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="R8G18_000416"
     CDS             15689..17779
                     /locus_tag="R8G18_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEE9794215.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="R8G18_000417"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="R8G18_000417"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HEE9794216.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="R8G18_000418"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="R8G18_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HEE9794217.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="R8G18_000419"
     CDS             complement(19747..20349)
                     /locus_tag="R8G18_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HEE9794218.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="R8G18_000420"
     CDS             complement(20339..20506)
                     /locus_tag="R8G18_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HEE9794219.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="R8G18_000421"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="R8G18_000421"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HEE9794220.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="R8G18_000422"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="R8G18_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HEE9794221.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="R8G18_000423"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="R8G18_000423"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HEE9794222.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="R8G18_000424"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="R8G18_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEE9794223.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="R8G18_000425"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="R8G18_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HEE9794224.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="R8G18_000426"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="R8G18_000426"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEE9794225.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="R8G18_000427"
     CDS             28334..28501
                     /locus_tag="R8G18_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HEE9794226.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28745..30133
                     /gene="frlA"
                     /locus_tag="R8G18_000428"
     CDS             28745..30133
                     /gene="frlA"
                     /locus_tag="R8G18_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HEE9794227.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            30154..31176
                     /gene="frlB"
                     /locus_tag="R8G18_000429"
     CDS             30154..31176
                     /gene="frlB"
                     /locus_tag="R8G18_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HEE9794228.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31226..32057
                     /gene="frlC"
                     /locus_tag="R8G18_000430"
                     /pseudo
     CDS             31226..32057
                     /gene="frlC"
                     /locus_tag="R8G18_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32054..32839
                     /gene="frlD"
                     /locus_tag="R8G18_000431"
     CDS             32054..32839
                     /gene="frlD"
                     /locus_tag="R8G18_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEE9794229.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32939..33670
                     /gene="phoB"
                     /locus_tag="R8G18_000432"
     CDS             32939..33670
                     /gene="phoB"
                     /locus_tag="R8G18_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HEE9794230.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33822..34907)
                     /locus_tag="R8G18_000433"
     CDS             complement(33822..34907)
                     /locus_tag="R8G18_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEE9794231.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(34919..36223)
                     /locus_tag="R8G18_000434"
     CDS             complement(34919..36223)
                     /locus_tag="R8G18_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HEE9794232.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36235..36588)
                     /locus_tag="R8G18_000435"
     CDS             complement(36235..36588)
                     /locus_tag="R8G18_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HEE9794233.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36599..37477)
                     /locus_tag="R8G18_000436"
     CDS             complement(36599..37477)
                     /locus_tag="R8G18_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HEE9794234.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(37474..38700)
                     /locus_tag="R8G18_000437"
                     /pseudo
     CDS             complement(37474..38700)
                     /locus_tag="R8G18_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            complement(38700..39863)
                     /locus_tag="R8G18_000438"
                     /pseudo
     CDS             complement(38700..39863)
                     /locus_tag="R8G18_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            complement(39947..40309)
                     /locus_tag="R8G18_000439"
     CDS             complement(39947..40309)
                     /locus_tag="R8G18_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEE9794235.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40326..41231)
                     /locus_tag="R8G18_000440"
     CDS             complement(40326..41231)
                     /locus_tag="R8G18_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794236.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="R8G18_000441"
     CDS             complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="R8G18_000441"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEE9794237.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42388..43146)
                     /gene="gph"
                     /locus_tag="R8G18_000442"
     CDS             complement(42388..43146)
                     /gene="gph"
                     /locus_tag="R8G18_000442"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEE9794238.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="R8G18_000443"
     CDS             complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="R8G18_000443"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEE9794239.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43834..44670)
                     /gene="dam"
                     /locus_tag="R8G18_000444"
     CDS             complement(43834..44670)
                     /gene="dam"
                     /locus_tag="R8G18_000444"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HEE9794240.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44777..46063)
                     /gene="damX"
                     /locus_tag="R8G18_000445"
     CDS             complement(44777..46063)
                     /gene="damX"
                     /locus_tag="R8G18_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HEE9794241.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="R8G18_000446"
     CDS             complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="R8G18_000446"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEE9794242.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="R8G18_000447"
     CDS             complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="R8G18_000447"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HEE9794243.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="R8G18_000448"
     CDS             complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="R8G18_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HEE9794244.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="R8G18_000449"
     CDS             complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="R8G18_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HEE9794245.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="R8G18_000450"
     CDS             complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="R8G18_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HEE9794246.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="R8G18_000451"
     CDS             complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="R8G18_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HEE9794247.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="R8G18_000452"
     CDS             complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="R8G18_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HEE9794248.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51628..54180
                     /gene="mrcA"
                     /locus_tag="R8G18_000453"
     CDS             51628..54180
                     /gene="mrcA"
                     /locus_tag="R8G18_000453"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HEE9794249.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="R8G18_000454"
     CDS             complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="R8G18_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HEE9794250.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55225..57360
                     /gene="igaA"
                     /locus_tag="R8G18_000455"
     CDS             55225..57360
                     /gene="igaA"
                     /locus_tag="R8G18_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HEE9794251.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            57425..58093
                     /gene="yrfG"
                     /locus_tag="R8G18_000456"
     CDS             57425..58093
                     /gene="yrfG"
                     /locus_tag="R8G18_000456"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HEE9794252.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58104..58505
                     /gene="hslR"
                     /locus_tag="R8G18_000457"
     CDS             58104..58505
                     /gene="hslR"
                     /locus_tag="R8G18_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HEE9794253.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58530..59408
                     /gene="hslO"
                     /locus_tag="R8G18_000458"
     CDS             58530..59408
                     /gene="hslO"
                     /locus_tag="R8G18_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEE9794254.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59543..60916)
                     /locus_tag="R8G18_000459"
     CDS             complement(59543..60916)
                     /locus_tag="R8G18_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEE9794255.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(60929..61243)
                     /locus_tag="R8G18_000460"
     CDS             complement(60929..61243)
                     /locus_tag="R8G18_000460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEE9794256.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            61622..63244
                     /gene="pckA"
                     /locus_tag="R8G18_000461"
     CDS             61622..63244
                     /gene="pckA"
                     /locus_tag="R8G18_000461"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEE9794257.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="R8G18_000462"
     CDS             complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="R8G18_000462"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HEE9794258.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="R8G18_000463"
     CDS             complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="R8G18_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEE9794259.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65616..66092
                     /gene="greB"
                     /locus_tag="R8G18_000464"
     CDS             65616..66092
                     /gene="greB"
                     /locus_tag="R8G18_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEE9794260.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66189..68510
                     /locus_tag="R8G18_000465"
     CDS             66189..68510
                     /locus_tag="R8G18_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEE9794261.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            68948..69175
                     /gene="feoA"
                     /locus_tag="R8G18_000466"
     CDS             68948..69175
                     /gene="feoA"
                     /locus_tag="R8G18_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HEE9794262.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69192..71513
                     /gene="feoB"
                     /locus_tag="R8G18_000467"
     CDS             69192..71513
                     /gene="feoB"
                     /locus_tag="R8G18_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HEE9794263.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71513..71749
                     /gene="feoC"
                     /locus_tag="R8G18_000468"
     CDS             71513..71749
                     /gene="feoC"
                     /locus_tag="R8G18_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HEE9794264.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            71952..>72510
                     /locus_tag="R8G18_000469"
     CDS             71952..>72510
                     /locus_tag="R8G18_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEE9794265.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
BASE COUNT        17433 a        19699 c        18348 g        17030 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctatggg
    28801 aagccaggaa ctccaacgca agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac
    28861 aaccgtcggc tccggtattt ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc
    28921 gtggcttacg gtgctcgcgt ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt
    28981 ctatgcggaa ctatccaccg cttatccgga aaatggcgca gattatgttt atctgaaaaa
    29041 tgccggaagc cgaccgctgg ctttcctctc cggctgggcc agcttctggg ccaacgatgc
    29101 accgtcattg tcgattatgg cgctggcgat tgtcagcaat cttggctttt taacgcctat
    29161 cgatccgttg ctcggtaaat ttatcgccgc cggattaatt atcgccttta tgttgctaca
    29221 cctgcgctcc gttgaaggcg gcgcagcgtt tcagacgcta attaccatcg ccaaaattat
    29281 cccgttcact atcgtcattg gccttgggat cttctggttt aaagcggaga attttgccgc
    29341 ccctaccacc actgcgattg gcgcaacggg cagctttatg gcgctgctgg cggggatctc
    29401 tgccaccagt tggtcgtata ccggcatggc ctctatctgt tatatgaccg gcgaaattaa
    29461 aaaccccgga aaaaccatgc cacgagcgct gattggttcc tgtctgctgg ttctggtgct
    29521 ctacaccctg ctggcgctgg tgatttccgg cctgatgccc ttcgacaaac tcgccaattc
    29581 tgaaacgccg atttccgacg ccctgacctg gatccccgca ctcggcagca ccgctgggat
    29641 ctttgttgcc atcacggcga tgatcgtcat tcttggttcg ctttccagct gcgtgatgta
    29701 ccagccgcgg ctggaatacg cgatggcgaa agacaacctg ttctttaaat gcttcggcca
    29761 tgtgcatccg aaatacaaca cgccggatgt ctccatcatc ctgcaagggg cgctggggat
    29821 cttcttcatc ttcgtttccg atctcaccag cctgctgggt tatttcaccc tggtgatgtg
    29881 tttcaaaaat accctcacct tcggctccat catctggtgt cgtaaacgcg acgattacaa
    29941 accgctgtgg cgtactccgg ctttcgggct gatgaccacc ctcgccattg cgtcaagcct
    30001 cattctggtc gcctcaacct ttgtctgggc accgattccc ggccttatct gcgccgtcat
    30061 cgttattgct actggtctgc ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa
    30121 cgctttgtcg taatgttatc tggagaaaat aaaatgttgg atattgataa aagcaccgtg
    30181 gactttctgg tcaccgaaaa tatggttcag gaagtggaaa aagttctcag ccatgacgtt
    30241 ccgctggtgc acgccatcgt ggaagagatg gtgaagcgcg acattgatcg tatttatttc
    30301 gttgcctgcg gctcgccact caacgcggcg caaacggcga aacatctggc ggatcgcttt
    30361 tccgatcttc aggtctacgc catttccggc tgggagttct gcgataacac cccgtatcgc
    30421 ctcgacgatc gttgcgcggt gattggcgtt tctgactacg gtaaaacgga agaggtaatc
    30481 aaagcgctgg agctgggccg ggcctgcggc gcactcactg cggcgttcac caaacgcgcg
    30541 gacagcccga ttacctcggc ggcggaattt agcattgatt atcaggccga ctgtatctgg
    30601 gaaattcacc tgctgctctg ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg
    30661 aacgcggaaa tcggcaagat caaaaacgat ctcaagccgt tgccgaatgc gctcggtcat
    30721 ctggtacgca cctgggaaga aaaaggccgc cagcttggtg aactggccag ccagtggccg
    30781 atgatttata ccgttgctgc gggtccgctg cgtccgctgg gttacaaaga aggcattgta
    30841 acgctgatgg aatttacctg gacgcacggc tgcgtgattg agagcggaga gttccgccat
    30901 ggcccgctgg agattgtcga accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa
    30961 agtcgccaca ccaccgaacg cgccattaac tttgttaaac agcgtactga caacgtgatc
    31021 gtcatcgatt acgccgaaat ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg
    31081 ttcgtgccaa tggagtggct ctgctactac ctgtctattt acaaagatca caacccggat
    31141 gaacgccgct attacggtgg tctggtggaa tattaatccc tctcccggcc cggcaacggg
    31201 ccggatttac gcaaggagtt acccgatgaa aacaggtatg tttacctgcg gccaccagcg
    31261 gctgcctatt gaacacgcat ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat
    31321 ttggggcggt cgcccgcacg cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat
    31381 caaggcgctg gcgcagacgt atcagatgcc gattatcggc tatacgccag aaaccaacgg
    31441 ctatccgtat aacatgatgc tgggcgatga acatatgcgt cgcgaaagcc tcgacatgat
    31501 caagctggcg atggatatgg caaaagagat gaacgcgggt tatacgctga tttccgcggc
    31561 ccacgcaggc tatctcacgc cacctaatgt tatctgggga cggctggcag agaacctaag
    31621 cgaactgtgt gagtacgcgg aaaacatcgg catggatcta atcctcgaac cgttaacgcc
    31681 gtatgaatcg aacgtcgtgt gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc
    31741 ttcgccgcgc ctgttcagca tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt
    31801 gatgagttat ttcgacaaac tgggcgataa attatcgtca tctgcatatt gtcgacagcg
    31861 acggggccag cgacacgcat tacattcctg gagaaggcaa aatgccgctg cgggaactga
    31921 tgcgcgatat tattgagcgg ggctatgagg gttactgtac ggtggagctg gtgacgatgt
    31981 atatgaacga gcccagactc tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc
    32041 cggaggatga gagatgaaaa ccctggcgac aatcggcgat aactgcgtcg atatctaccc
    32101 gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg
    32161 ctacggcata cagccgggat gcattacctt ggtgggtgac gatgactacg gcacaaagct
    32221 gaagcaggat ctcgcccgca tgggcgtcga tatcagccat gtccatacga aacacggcgt
    32281 taccgcacaa actcaggtgg aactgcacga caatgatcgc gtttttggcg actacaccga
    32341 aggcgtgatg gccgactttg ccctgagtga agaggattac gcctggctgg cgcagtatga
    32401 cattgtgcac gcggcaatct ggggacatgc ggaagacgca ttcccacagc tgcacgctgc
    32461 gggcaaactt accgctttcg acttctccga caagtgggac agcccgctct ggcagacact
    32521 ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct
    32581 gaagatgaaa gcgattgttg cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa
    32641 cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt
    32701 tatcgacacc atgggtgccg gagattcgtt cattgccgga ttcctttgcg gctggtctgc
    32761 ggggatgaca ttaccgcagg cgatggcgca gggaacggcg tgcgcggcga aaaccattca
    32821 gtatcacggt gcctggtagg tataacgttg gcgtgagcat ctccgcgcca acgtgctgtt
    32881 acttgccgga aaacgaccct ataatccgtg taattcattc tttatttcag ggtcgattat
    32941 gtcagctacg gaccgctact ctcatcaact cctctacgct accgtccgcc agcggctgct
    33001 ggatgatatc gcgcaggggg tttaccaggc cgggcaacag atccctaccg aaaacgagct
    33061 ttgtacgcaa tataacgtca gccgcattac tattcgcaaa gctatcagcg acttagtggc
    33121 agacggcgta ctgatccgct ggcagggcaa aggcaccttt gtacaaagcc agaaagttga
    33181 aaacgccctg cttactgtca gtggttttac cgattttggc gtctcacaag gcaaggcgac
    33241 gaaagagaaa gtgatcgaac aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa
    33301 catccccgga aacagcgaag tgttccatct ctgccgggtg atgtatctcg ataaagagcc
    33361 gctgtttatt gatagttcat ggatcccgct gtcgcgttat cctgactttg atgagattta
    33421 cgtcgaagga agctccacct atcagttatt tcaggagcgt tttgacacgc gagtggtcag
    33481 cgacaaaaag accatcgata tctttgccgc cacccgcccg caggcaaaat ggctgaaatg
    33541 cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc
    33601 ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc accttaacga ttgataataa
    33661 aagacattaa ccgtaggccg gataagatgc gccagcatcg catccggcga tgctggcgcg
    33721 ttgaatttta catcccgtac gttcccctca ccctaaccct ctccccaaag gggcgagggg
    33781 accgttcagt acggaattta gcttgacggt gctatccagc attaaatact ggcaatactc
    33841 tcacgcaaaa tccgcagcac cgtctcttca ccgttgcgat tcgggttaat gcgaatcgca
    33901 caatgttctg attgtggatt cgcctggcga aacgttccgg aaaggcgata aaagagcggc
    33961 gggatttcat atttcgactc tgcacccacc gggtaaggca aggcaccgcg cttttgcgcc
    34021 tcttccagca ctctggcggc aatcggctga tgaaactcga caatcaacac cttcgactgc
    34081 gcattagcaa tcaccgcgct tttcacttcc ggcaccgcac caccgttaag caaagccagc
    34141 aaccgttcag atacccccgc ctgcaccgcg tgcatcactg gcgcaaacac cagcccacac
    34201 aagacttcca gcgcctgagc gccctgaatc tggctgccgc cggaatagag cgtggagcga
    34261 atccgactaa taacatcggc atcgcctacc accgcgccaa caccctccgg cccaaacagc
    34321 ttgaagcagg aaaaagtcga gacatttgcc ccgcattcgc agccaattcg cgccactttc
    34381 atcaccgcat agttgtcatc ggttaatgca ggaacgccag ccgagcgcaa tgttgccagc
    34441 acatctgcca gaatgtagcc gtcctgcggc tgctggcgcg tatgctgcac cagcgccgca
    34501 tccggtagtt gctcgtcgac gacctgcttc agtgccgaca ggtcattgaa atcagcagta
    34561 ataagcgtca gccccatctg ctcaataatg acctgtgtcg tcgggtaaac aggcgcatca
    34621 tgtaccagaa gacgctgccc cggtttgagc aaagccgcca acgccgcacg aatcgcgcca
    34681 gtacccgcac cctgcaccag cgccgcagcc tgtgcgtgaa atgcatcagc cagcacctgt
    34741 tccacacgct gggtaacgcg cggttgattc agtcctggcg ttaagcctaa atcaccgcca
    34801 gtaagaaact cgctgccggg gaaatggcga caaatgctat ccaccagcgc aaactgcttt
    34861 tgctgcgcct caataatcgt cagactttgc agaggaaacg tcttcatagg tgccttcctt
    34921 aagccggaac gaacaggcca agccagtaaa gaacattcag cagaataccg gtgatcatta
    34981 ctgccacgac cggtgccgcc attttctgta ccggacggcc cagggattcg ttgaggaagt
    35041 aaatggcaac cgcgatagag aatccggtat aacccgccat cttaattgct gcgaaaatcg
    35101 aaccgaccag cagcgccact tccatcagca tattcatggc gttacggatg ttatccgacg
    35161 cattacgcac cgacgggtag cgtcccagcc atttgccgat cgaacgaagc agcaagactt
    35221 ccgccgaaat aaccactgcg cctaataccg ctgcaactat cggattcggc gagagatagc
    35281 ccaccgcata aacaaaggta aagcccgcaa ctgcatacac acccgttgct aaggcggtgg
    35341 tggcaatcaa cggtacaaaa cccagcccgc gcataaattc tgccagtgcc gcctggttga
    35401 tcagcgtttg cgattgttcc ggcgttacgc ctgcggagta cgctttctcc agagtgaaga
    35461 tcgacacttc actgccggcg aaaatcttca tgctggcaac ggcggcaatc aatgctccca
    35521 cgatggcgat atagggtaag tttttgataa tccgcgacgt gcgttcttca aacaccgaaa
    35581 gcccgctggc atcaatatca ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc
    35641 ccagcagcat caccatgcca ataaagattt cgatggattc agggttaaga tgtgggaaat
    35701 agcgcacgac taccacacgg gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc
    35761 agccaaactg gtagaaaatc gccaccagcg ggaacagtgc aaaagcagaa accaccggcg
    35821 agcttaattc cccgaggcta cctaatacat ccaccggcag cgcggtcagc agctggttta
    35881 ctggcaacag gcaagtaagg atcaacacgc cccagatagc gccaagacca aacgccatca
    35941 ggctgtttat cgccagtacg ccgaggatat cggtaggaag aaagagtaac catgcgttga
    36001 gcagcccggt tttcagggta aaagagatcc ccaccgaggc cacaaaacca atgctcagac
    36061 caaaagcgat actccccgcc tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga
    36121 tcgggcggat accgtcatga aaaacagccg ccgagcgatg cgccagaagc gatgtcatac
    36181 ccgtcaggca cgccaccacg ataatctgaa tatacagatc catagtcgtg ccttttattt
    36241 cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc
    36301 cacttttcct tcagcgatca ttttggcgat atgctcgtct ttcgctttaa tgcctggttt
    36361 ggcaatggtg caacttttgt tatagccaat caccgcgatg gcaatcgaca atgccgcgcc
    36421 cgcaccggta ttacacgcac cgatgtagta atccagttgc ccggatttca ctttcattgc
    36481 cgcttccatg tcgttgtgaa tgaaaacttc aaaactgcca ggagccgtcg cttcaatggt
    36541 ttttttaatc tgctcacgct gtaagcctgc aacgccaatc tttttcatga gtctgtcctt
    36601 attggaaaaa ttgagaggga gtttcacgta acatcacatc cacatcggcc tgactgaatc
    36661 ctgactggcg caattgcgga ataaaggtgg tcaataaata gtcatagcca taaccaccat
    36721 tggcttttaa atgggagcgg cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc
    36781 cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc gtccgggtag taactgttct
    36841 taccaatggt gtcgaactgc acgtacgcgc cgagatcgat catcttcaaa atgttgtcga
    36901 ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga aagatcaact ccgtgggctt
    36961 gcaacagagc cagttgttcc agccccatgg tgctgaacga cgtatgcgtg gagatcgggc
    37021 gtccggtctg gttatgcgcc agcgcagcgg caataaatac cttctcttcc agcggcgtaa
    37081 tctttccttc gctggagccg atctccgcga tgatcccggc tttcagctcc gtgccatcga
    37141 taccctgttc aatttcatcg accatctcct gcgccagttc ctgcacgctg cgggtcacca
    37201 catgttccgg gaaaaacgca tcctggtaat aaccagtaca ggccaccacg ttgatcccgg
    37261 tttcgcgcat tacaccgagc ataaattgcg cattgcgccc catgtaacgg ttggtcatct
    37321 caatcacatt acgtacaccc cgggtcatca ggtcgttcat ctcctggcaa atgaacgcat
    37381 actgatcaag gcggcagtcc acgttgtttt taaagccgga gagatcaata tgcagatgct
    37441 catgggccag ggtgtaaccc gtcggatcaa aactcatagg gtgtctcctg caaaccggag
    37501 ggaggaaaga aaagagcgac cattttgtgg cggtggcgca cggaaaaatt cacacacggt
    37561 agctcccaca tcagaaagcg tggtgcgcac accgagctgc gtagcagcca ggccttgctg
    37621 ataaaccagc agcggcacca cttcgcgggt atggtggctg tgaccaatgg tcggatcgtt
    37681 gccgtgatcc gccatcacga ccaggcaatc atctggctgc atcgcatcaa caagccgggc
    37741 aaggttacgg tcaacgacct gcaaacgttc ggcataacgt gcgacgtctt ctgcatgacc
    37801 agcgaggtcg gtttcctgga tattggtgca aataaacgcc gtcggatggg tgttaaattc
    37861 gtcgagggtg atatccataa tccgctggct atccaccagg ttttgccagc tcacgccata
    37921 aggattgctg acgatatctg ccaccttacc caccagcacg gttggcacgc ccgcctcata
    37981 cagtttttgc ggcacctgca cttttttatc gacaccgtag cccatatgca cgacctggaa
    38041 gccgttgtca taagcgccag aacgcggcgc attgatacca ataaagcgcc cttctttgct
    38101 ttctgcggca tcgagaatgc gttgactgtc ggttaacagg ccaccaaatg taatgacccg
    38161 accgacctgt acctgctcac gcacgatacg accaattttg attgcgtcgt caaaagagat
    38221 cacagagaga ttggcggtaa tgttatagac ttggcctaaa tccgcctcga gattatcgcc
    38281 aatcgcaacc gcctgattga cccacagaaa ttacagttca tcgccacggc gctccacctg
    38341 ccaaccggcg gatactaatg cctgctcaac acggtcaatc acatcgcgaa aaggcatccg
    38401 cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc cccataaagg tatcgccacc
    38461 ttcatgttgc agctctgcca cgccccaggt tgcggaatct gacggctgca tatcgcctgg
    38521 cgcataaccc aatgcgttga ttagccccag cttctccagc gttggtagct gcaaatgcgg
    38581 caactggctc aggatgtgac cacatgtatt cgctcccgca tcttgcggac gcaccagcgt
    38641 gacatctttc attgccccta cgccaaagct atcaatcact aacaccacaa atcgcgccat
    38701 tacgccccca gagagtttcc aagactgtca taacgaccaa ctattttcgg ttcgccacgg
    38761 tgaataccgg acaccagcac cacatcgcta cgggtgacaa aaatctgcgt gcggaaacag
    38821 agcaccactg cactgcttac cggaaactcg cctgccagcg acagggtgta gtcgatactg
    38881 ctgtcatcca cagttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc
    38941 aacgcatgtt gcgcatgacc acgacggtag taaccgccgc cgtagcagta gctgtcgcca
    39001 cggaaatgat gggagatttc actcaaccag agcatcgcga tacgttcaga ctgatcgccc
    39061 tgctggtttg ccggaatagt gcccgtcagc gcatgaccgg gttcggcatg agtcacaccg
    39121 tattacgcca gtaatggcag cgaagtgcag ctggtcgctg aaggcgcgtt cagttgctca
    39181 agtgcaatac cagatttcgc cagttgatcc cgtgcctgta tcagcgtgtg aagattcggt
    39241 gtcggcaaaa cttttccggc agcctcatcc caaagcaggc aagggaaatg ggtaagtccg
    39301 gctaaatgca gccccggcag tttcttaatt tcagcaacca cctcgtgcag cgaatgctgt
    39361 acaaaaccgc tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc
    39421 acaaactgca cacgtccggc cttcaccgct gccgcagaaa cttcccgcgc tttgtcgaga
    39481 gtaaacacgg tgatgacgtc ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg
    39541 atttgtacca gatgcccctg atgtgccacc gacaaaccag cgcggcgcat gactcgcgcc
    39601 tctttgtaat ccaccgccac aatgccgctg taacctaatg ccagcaattt ttccgccagc
    39661 catgggttac ggccaaactg cttggtcatc agatacagtt caatgccgta aagccgcgcc
    39721 gtctcaatca gtcgcttacc gttttccagt acctgatcca cgtcgatcac ccagctgtcc
    39781 ggggcgatct tgccctgctg ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg
    39841 cgtttcaatg cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc
    39901 atttttttga atatttaggt tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt
    39961 tagcagccat ccataatcca taaaggttgg ccagcaaata gccttcttca cacgggttaa
    40021 cttccagtgc gaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc
    40081 tggattgcgc cagctctgcc agcaattcgt tatccagcgg ctctatttct tcaccacggc
    40141 gactgcgcat cagtgcactc gccatatgtg tcatcgccat cgttccttgc tcactgcgca
    40201 ccggcagatg gcactctgtt tccagtacgt tgacgacctg catcatgcct ttgcagatgt
    40261 ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct ggtttccata tcagatgcct
    40321 ttcgtttaat aactcggttc ctgttcccca ctcactaccc gttgttgatg ggcgagcagg
    40381 gcgtgtttat ctacaacggc acgcagcagt tgttgcattg ggtaatcatc gactcgcgtc
    40441 aggaccacgg cttcggtggc ctgtaaaaat cgcggatcgt ctgtgagcgg cgtcgcctct
    40501 aatcccagca tggtcagttc gttttccgcc accacgttcc agataaccgc atcgacatcg
    40561 cctttaacaa tgcgttgtaa actctcgtga taagagagat cgacacgttc cacatcacta
    40621 tcgccaaaaa agacatcggt catgattttc tgatccgccg aacggttatc cagccccacg
    40681 cgcttcacgt ttgcggactc gcctttacgg caaatcaact ggtgctcgcc aacgtaggtg
    40741 tgcggcccca actccagcgc gaggcataac cctttttgcg tgagataact ttccgccgcc
    40801 agtcgggaaa ccaccgccat gtcatacacg ccgttaagta aacactccac gcgaatatcc
    40861 gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa actgagcttt cagcccgctc
    40921 gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg cacataccac gttgttgata
    40981 tccacatgac tcagcaatgc tttgttatcc atctcgacca gataactgcc attgcgccca
    41041 cggcgttcaa tccgtatcgc tccgcttgat tccaacgttt tcaacgcggc ctgcgtcagg
    41101 ccaaccgatg aacggcattc agttgccagc tcatctatgg ttttcagtcg attaccgcat
    41161 ttttcaccca acaaataacg ggccagcgtc gtgatgacga cgccttcttt tttgataaac
    41221 gttcgacgca taatatattt tcaataaaat gaatatttat atcttcatta aaatgaagat
    41281 aagtggcaac gacgagatgt ggaaacggcg aggcgcttca cattttttcg gattattgca
    41341 aatgaggata aaaaaaccgg gtttccccgg tttttggcat taatgttaaa ttacggcttc
    41401 gccacaaaac caatcgcttc gtacaccgct ttcagcgtac gggaagcgtg tacactggct
    41461 ttttctgcgc cgtctttcat cacctgttgc aggaaggctt catcgttgcg gaaacggtga
    41521 tagcgttcct gcaattcagt cagcataccg gaaacggcat cagccacttc acctttcaga
    41581 tgaccataca tcttgccttc gaactgtttt tccagttctg ggatgctctg gcccgttacc
    41641 gcagaaagga tatccagcag gttggaaacg cccgctttgt tctgcacatc gtagcgaact
    41701 accggcggct cgtcggagtc agtgaccgcg cgtttgattt tcttcactac cgatttcgga
    41761 tcttccagca ggccgataac gttattgcgg ttatcgtcag acttggacat cttcttggtc
    41821 ggctccagca gcgacattac gcgcgcgcca gatttcggaa taaacggctc cggcacctta
    41881 aagatctcgc catacagcgc gttgaaacgc tgggcgatat cgcgactcag ttccaggtgc
    41941 tgtttctggt cttcacccac cggtaccaga ttagtttgat acagcaggat gtccgcagcc
    42001 atcagcaccg gatagtcaaa cagaccagcg ttgatgttct cggcataacg cgcagattta
    42061 tctttaaact gcgtcatgcg actcagttcg ccgaagtagg tataacagtt cagtgcccag
    42121 cctaactgcg catgttctgg tacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg
    42181 ataccacaag ccagatacaa ggccagcgta tccagcgtcg ctttacgcag cttctgtgca
    42241 tcctggcgca cggtgatcgc gtgttggtca acgatacagt aaatgcaatg gtagtcatcc
    42301 tgcatgttta cccactgacg cagcgcaccc atgtagttac caatggtcaa ttcacctgag
    42361 ggctgtgcgc cactaaaaac gatgggctta gtcatttttc gattcctgat tttcgctatg
    42421 cggaagccct aatgcgggca gaaggtcatt tatagactga taaattacat caggctggct
    42481 gagatcgata gcctcgccgt agttatatcc gtaggttaag ccaactgatg ggcaacctgc
    42541 cgcttttgcc gcctgaatat cattgcgtga gtcgccgaca aacagcatct gttgtggggc
    42601 aattcccatc cgctcagcca ccagtaacag cgggtccgga tgcggttttt tgttttgcac
    42661 atcatcgccg ccgataacca cgctaaagta tttggcaata tctaaggctt cgagcagcgg
    42721 tgcgacgaac ggcgtcggtt tgttggtgac caggcctagc ggcaggcctt tagcctgcaa
    42781 cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc ccctcttcgg caacctcgcc
    42841 atagtagcga tcgaacagtt tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc
    42901 atcaacgggc ggtttaccca ttgttttacg ctgagtcgca cgttcctgac gcgcccaggt
    42961 caatgcgcgc tccatcagaa catctgcgcc gttaccaatc caggtaataa cgcgttcttc
    43021 acctgcgatg ggcaactcca gcgcatacag cgccatatct accgcagcag caagaccggg
    43081 agcactgtcg accagcgtac catcaagatc aaaagcgacg ccgcgaatat cttcaaactt
    43141 attcatgact tacctttgcc agttcactgc gcatttcatc aatgactttt ttgtagtctg
    43201 gctggtcgaa gattgccgaa ccggcgacga acatatccgc gcccgccgca gcgatttcgc
    43261 caatgttgtt caccttcacg ccaccgtcca cttccagacg aatgtcaaag ccagactcgt
    43321 cgatacggcg acgtacttcg cgcagtttat ccagtgtttg aggaatgaaa gactgaccgc
    43381 cgaaaccagg attgacggac atcagcagga tcacatccag cttatccatc acgtaatcca
    43441 gatagctcag aggtgtcgcc gggttaaata ccagacccgc tttacagcca ttttctttaa
    43501 tcagttgcag cgtgcggtca acatgctcgg aggcttctgg atgaaaggta atgatgctgg
    43561 caccagcggc agcgaaatca ggcacaatgc gatcgacggg tttcaccatc agatgtacgt
    43621 cgataggggc ggtaatgcca tagttacgca aggatttcag caccattggc ccaatcgtca
    43681 gattgggaac atagtggtta tccatgacgt caaaatgcac gacatcagcg ccagctgcca
    43741 gggcttttgc ggtatcttca cccaggcggg caaaatcagc cgacagaatt gagggggcaa
    43801 tcaaatactg tttcatccgc ttctccttga gaattatttt ttcgcgggtg aaacgactcc
    43861 tggtttgtac aaagccagca gttcgtccac ctttttacgt gtgccgccgt tgctgcttat
    43921 actgcgtcga actttgacga catgcaattt tgcgcgctga taccactcac gcgttaacat
    43981 cgtatcgtga ttggagatca gcactggaat atggcgctca accagacctt cggcgatctc
    44041 cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg tgatacgccg taaagttggc
    44101 ggtcgcagac agcggtgcat aaggcggatc gcaatagacg acggatgcat catctgcgcg
    44161 cgccatgcta tcggcgtaag actcacaata gaaaaaggca ttctgcgctt tttcagcgaa
    44221 gtgatacaac tctgcttccg ggaaataggt ttttttgtag cggccaaacg gcacgttaaa
    44281 ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg tagcggttca aatataaaaa
    44341 cagtaccgcc cgacggaacg gatcctggct tttgttgaac tcttcgcgga actgatagta
    44401 aacctcggcg caatttgttt cgggaacaaa cagctcgcgt gcggcctgta cgtactcatc
    44461 agtacgcatc ttcacaatgt tatagagact gatcaggtcg ctattgatat cggcaaggat
    44521 ataacgagaa aagtcggtgt tgagaaacac cgacccggca cctacaaaag gctcaaccag
    44581 acattcgccc ttgggcaaat gccgtttaat atcatcaagc aggggatact tgccccctgc
    44641 ccacttcaaa aaagcgcgat ttttcttcat gctgactaac taattacacc ttctccggct
    44701 gtggagaaag ctccgacagc atccagcgct tcaggcagct cccgcaacga taaaccattg
    44761 cgggagataa ccttgattac ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg
    44821 ggtttttggc ctgaacatct gctggcaatg tagataccgc ttttttcgcc tcttctttcg
    44881 aagcgtacac gccagaaacc aggacatacc acggctgacc attacgcgcc gtttcataga
    44941 caacgtagtt tttcagattc tctttcttcg cccaaccgtt caggttgtcg tagttagagg
    45001 aactgctcag ctgcagagtg taatggctgg acggtgccga tttcaacgaa ccaacattac
    45061 ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg cgccggggat gccgtctgta
    45121 ctggagccgt agtcgccgtc tcttttggtg ctggcgtaga agtcgcagtc ggtgccttcg
    45181 tgctcgccac aggggcagct ggttcagtac gcttcggcgt ctgtgctacc ggcttcggct
    45241 ccgttttcac ggttgcttgc ggttttttcg gttcaatcac cgcctgctga cgagctgggc
    45301 gcgtagtgga cggacgttca gcggtttgcg ttttcgccgt gtcacgcgat gcattgccat
    45361 tgcgaacagg cgcgacagtc gcgggttcgg tcggcaatgt ggaattgacc gccacattgt
    45421 tcaactgttg ctgattttgt ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa
    45481 cacgttgttg accatccgtt gccgccgggg tttgcccttg agtcggcgta gaagagatcg
    45541 gtggcagaga aacatcctgc tgagtatttt ccgcagacgt ggttcccggc gctggctgca
    45601 ccccattcgc ctgatcggtc gcattgcctg caagatcaat actcttctcg ccagacgcgg
    45661 tttgatcgct ggaagaggtc gagggggctt ttagcgcaga accgataccg atgatcaaca
    45721 gcagtagaac cagaatgccg acgcccatca tcatatactg acgagaagcg ggtttactgg
    45781 ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc ttcatccacg gtttcatctt
    45841 cggattcgtc aatttcttct tcgatttccg gttcctcatt gcgctctttt tgcgcacgag
    45901 tcggacggcg atcgtcagtg tcatcaagtt caatatcatc aaaattgatc tgcggttcgc
    45961 cacgttcagt acgctcagaa cgttcagaag attgacgaga acgaccagta cgacgatcgc
    46021 tgggatcggg tttcagctcg tcttctggtt tgaattcatc catttaacac cccactaaaa
    46081 ggttaatgct taccacgttg caattaacct gaagctaata gaccgcttga taagcggcct
    46141 gacctttctt gttgttacgc tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc
    46201 gaaacgccgc tgcgaacttc actcttacca attgccaacg gaagaattaa gcgtatctct
    46261 cccgcaagga ctttcttgtc acgcagcata tgcggtaaat acgcctgcgc ggacatttcg
    46321 cgcggcccat tgaccggtaa cccagcccgc ttgagcaggg ttataatacg ctgcgtttcg
    46381 gcagaactaa actgcccgag acgttccgac gtccgcgccg ccatcaccat acccgcagcg
    46441 accgcttcac catgtaacca attgccatac cccatttcag cttcaatggc atgaccaaag
    46501 gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc gctcgtcggc ggcgacaact
    46561 tctgccttca gttcacaaca acggcgaata cagtacgcca ttgccggacc gtccagacgc
    46621 aacaacgcat ccagattctc ttccagccag ttaaaaaacg caccgtcaag aataatgccg
    46681 tatttgatga cttctgccag ccccgacgct aactcacgcg ggggaagcgt tttcagacag
    46741 tcgagatcca ccaccactga agcaggctgg tagaacgcgc caatcatgtt tttaccgagg
    46801 ggatggttga ccgcagtttt gccgccaacg gaggaatcga cctgcgacag taacgtcgtc
    46861 gggacttgaa tgaaacgaac accgcgctga taactcgccg ccgcgaagcc ggtcagatcg
    46921 cccactacgc cgccgccaag cgccaccagc gtagtatcgc gaccatgcgg cttttgtaac
    46981 aacgccgtaa agacggtatc gagtacagcc aggcttttat actgctcgcc gtcagggagg
    47041 ataacgctat cgacattaac acccgcctgt tcaagtacgc cgcggacctt atcgagatac
    47101 agaggagcca gggtttcgtt ggtgaccaac atgacctgct cgcccgattt cagcggtaag
    47161 aatgaagctg gttcattaaa caaaccagat gcgatggtaa ttgggtaact acgttccccg
    47221 agagtaacaa caatcctctc cataacgcga catccacctt aattactgta cccgcagacg
    47281 agtgtatata aagccagaat tagttgcttt ccagcatgtg aataatctgg tttgcaacca
    47341 ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc aatctcttca tacagcggat
    47401 tgcgttcatt ggccaacgct tccagaactt cacgtggcgg tgtttcaacg tgcagcaacg
    47461 ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa
    47521 cgacaacgcc acgagcggaa agacggttac gcgtttcacg ggatttcaca gagccaccgc
    47581 cagtagccag cacaataccc tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat
    47641 cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc cacatcagct ccggttcgtt
    47701 tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag ttgttgagct aactggcgcc
    47761 caatagtgct ttttccggca cccataggcc caaccagaaa gatattgcgt ttctctgcca
    47821 ttttttcggt actactaaga ctattcgtta atgataaacc cgcttcgctc agagagcgcc
    47881 gcaggacatg aactgaaacc tcataagata ttgcgagagt cagactgaaa attatctcaa
    47941 tactccagcg ggtttggcaa ctgaataaat cacccatccc tctgcatatc tggtcgctgc
    48001 aagcgcgctg ccttgctacc accgctctgg cgataaatca ccgggtaaga ttagcgtaaa
    48061 aaagacagca aaatgccgcc tgaatgataa atcatcatca tggaacgcag gcagcgaaga
    48121 gcaccaactc tcaaatcggt actccttgta tgctaaatac ctgcgcgcgt caaatagatg
    48181 aaacacgttc agcgtaaaaa cattaccgct tttacggctg tttactcact ggaaaccagt
    48241 cgtggcgtga taaacaccac taactcgcgt cgttcatctt cttttccgtc atgacgaaat
    48301 aattgcccga accagggaat gtcgccaagc aacggtacgc tatcctgacc cgatttattt
    48361 ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc ttttgacctc gacctgcgtt
    48421 tcgatctcct gcttatcaat cgccagcact tcgccatcgg cctgctgtag cacctgcccc
    48481 ggaacgttct ggctgatgtg taatttcagc cggatgcgac ctttttgtaa cactgtgggc
    48541 gtaacttcca tccccaggac ggcctcttta aattccaccg acgtcgcgcc actttccccg
    48601 ctggaaacct gatatggaat ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag
    48661 gccagcagac gcggactggc gataatatcc agctgctgtt tttgttcgag cgcggaaagc
    48721 tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc
    48781 gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc caacgccacc agcgtgttgc
    48841 gcatcggcca gcgtccattt cacgcctaac tcacgcaaac ttttttcatt aatggtgaca
    48901 atatgcgccg acagctcaac ctgcccgacc ggcagatcca tttgcgctac ccactgttca
    48961 agcgcgctta acgccgtttt gttatctcgt agcaaaaggc gattggtgcg tttatcgacg
    49021 gtcatactcc ctttggcact cagtagcttc tcccccgctt tcgccagttc tcccgcgtcg
    49081 gcgtattgca gggttatact gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc
    49141 tccgcctcct ggcgggcgat attgttattc tgccaggcaa tggaatgcac tgagagaatg
    49201 ttgccttcct gccgcgttat cagtccggcg cttttcacta cagtttgtag tgcctgcttc
    49261 cagggaacat ctgttagatg taacgacacc gtaccgctga cgtctggcga cacgaccagg
    49321 ttcaacttct cctgttcagc cagcgcctgc aacacctgag ctaccggaac gtcatccacc
    49381 atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg gtatcagcat caacagtagt
    49441 gcggctatcc attgcttcat ttgtatctcc ttgccgttgc cacaaccatt gtggcggttc
    49501 gcagtttgtc ccggtaccca gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc
    49561 gttttccagc acatcgtttt gctgcacccg tcgccatttc ttttgcccgt cttttattac
    49621 accgatgatg cgctcgcctc gccctaccat cccctgatag cgccactggc taagttcgct
    49681 aatccggcat agatcttccg gcggtttaaa agggtcacgc ataccggtta aaaggcacaa
    49741 tacaatacct gccaacaacc agcgtttaac cctcatgcgg cgtctccagt tgtagcgtga
    49801 acaaaagatc atcaccttcc acgcttaatg aaaaacggct cacgctgacg ttgcgctctg
    49861 ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcgcctc
    49921 cctgcgcgga tggatgccag gaaaccagtt gcgcgccggg caactgaaaa tccagtggcg
    49981 aaaagggcag cgttttttct tcgctgaagg gagtggtgtc aaccaggcga tacagtgtgg
    50041 cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg
    50101 tcgatgacag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc
    50161 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    50221 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    50281 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    50341 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    50401 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    50461 tgtcgctggc gctgccgttg caaacgctgc tcacgaattt gttggcgctc cagcaaacgt
    50521 ggtttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aacggtgttt
    50581 acgcgtgctt cggcgttgtc tgtcagacgc agtattagcg ttatcccgac ggccagcagc
    50641 agaggcgcaa cgaacatcag caaccagaaa cgaagaaaag cggtccggcg ttgctgtcgc
    50701 cagggcaaaa aattaattgg cgggttcatc agtacgcctt cccaagcgcc agccccagcg
    50761 cgatggcaaa gtctccacct ggcggcggta gcggcggctg acgaacagaa acggcctccc
    50821 agggatcaaa tccgccttcg ccacatatcg cgacgctttc gggatcaacg gataacgctg
    50881 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag cgacagcgtg
    50941 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    51001 aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcgctcaca tgaacacgca
    51061 acctttctgc cagagtgagt agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    51121 aggcgggact gagtgagtct tcgctgtaat cgaaacgcag ggagtccgga tccatatcca
    51181 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcaccg agggacatcg
    51241 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    51301 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagccgc tgcgcatcaa
    51361 caatccgccc atctttgata atgtcgttct ccagcggcaa ccgccaccag cgttgcaaaa
    51421 agcattcttt tgcgccccgc acgatcgcaa ccgctactgc ttcttgctgt tgtaaatgca
    51481 aaccaatttg ccagatctta aatgccattg tgatgatctc cttatcaccc gtcactctga
    51541 cgggtatatc aatgcgtctg gcttgccttt atactaccgc acgtttgttt ataaactgcc
    51601 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    51661 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    51721 caactgccgg atgtggcgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    51781 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    51841 gatcaaatcc cgccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    51901 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    51961 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa cttcttcctc
    52021 agtccagaac gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    52081 cagttgctga cgaaagacga gatcctcgag ctttatctga acaagattta cctcggttac
    52141 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    52201 acgctgaacg aaatggctgt gatagccggg ctgccgaaag cgccttccac ctttaatccg
    52261 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    52321 gaagggtata tcacccaaca acagttcgat cagacacgca ctgaggcgat taacgctaac
    52381 tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    52441 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    52501 acccgcaaag tgcagcaggc cgcacagcag gcggtacgta ataacgtgct ggactacgac
    52561 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    52621 gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct
    52681 gccgcagtca ccagcgccaa tcctcaggaa gcgacggcaa tgctggcgga cgggtcgact
    52741 gtcgcattga gtatggacgg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag
    52801 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    52861 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    52921 atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    52981 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    53041 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    53101 tctcgctggg atgcaggtgt cggttctgac tggcagccga agaactcacc accgcagtat
    53161 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt
    53221 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    53281 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    53341 caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctggtgga cccgtggttt
    53401 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    53461 tgcccggaat gcgatattcc ggtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    53521 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    53581 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    53641 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    53701 tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc
    53761 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    53821 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    53881 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    53941 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    54001 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    54061 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    54121 gcggtgcacg aggtgggaac aaccattatc gataatggtg aggcacagga attgttctga
    54181 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    54241 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc
    54301 ccataagcct gataatcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    54361 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    54421 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    54481 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    54541 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    54601 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    54661 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    54721 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    54781 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    54841 aacagtcggg aatgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    54901 ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    54961 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatt gtcctcaaat
    55021 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatatcc cgccttcaga
    55081 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac
    55141 gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga ccacgcctga
    55201 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    55261 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    55321 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    55381 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct
    55441 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    55501 cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    55561 gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga tgagaatacc
    55621 gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg
    55681 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    55741 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    55801 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    55861 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    55921 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    55981 tccctgcggg aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    56041 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    56101 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    56161 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    56221 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt
    56281 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    56341 ggcgcacaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    56401 ggcgatacat tgcgtatcag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    56461 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaacgac
    56521 gcccgctcat tgccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    56581 gcagttaatc gccagctgca ccctaaaccg gaagatggat ctcgcgtcag tgcctcatta
    56641 cgttcagcaa ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    56701 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    56761 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    56821 gatggcgtga atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca
    56881 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    56941 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    57001 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    57061 ctggcacaaa tgctgatgca tacgccgttt aacgccgagg gtatcgtcac aaaaatcttc
    57121 actgacgcca atggtacgca gcatattggc cttcatccaa tcccggatcg ttccggcctg
    57181 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    57241 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgaa gattcaggcc
    57301 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    57361 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    57421 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    57481 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    57541 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    57601 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    57661 ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg aagataccat
    57721 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca
    57781 tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt
    57841 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    57901 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    57961 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    58021 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    58081 ctcgctaatg tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata
    58141 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    58201 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    58261 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    58321 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    58381 agaaacgcga aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg
    58441 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    58501 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    58561 tgtttgaaaa ctttgccgtg cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    58621 tccttgagaa tcacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    58681 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcaag
    58741 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    58801 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    58861 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtggttggtc
    58921 tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc
    58981 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    59041 tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc
    59101 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    59161 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    59221 cctgctcgcg tgaacgttgc gccgacgcgc tgaaaacgct gcctgatgaa gaagtcgata
    59281 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    59341 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag
    59401 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgacg taatggcctg
    59461 atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat tattgttggt cagatacagc
    59521 gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    59581 ccgtcagatc cagttttcca tccttcgcag ctgtgagtaa tttttctttc gttatttcgt
    59641 tcggaagtaa actcatatca agcgcgtccc attcttttct gtctgagtca aagccataga
    59701 caatgactct gtcatcatta aaagcaaaca ggatccgctc cgcctgacca tcactattca
    59761 gatcttgctc gaccaggacg caggcgtctt tttcaataca ggtcatcacg ttatagcgat
    59821 cctggattaa ggccgaccag aatgtcgcat ccggtttaac agaaccagga gcaattaaca
    59881 cattttcggc taatactttt tccgatacct gttcctgaag atgttgctct ccatctaacg
    59941 ccatcagcag atcccgcgcg cgtttcgggt ctttcataaa cccggcatcg cttttcagcg
    60001 actcaagcgc cgcacgtcca tagcgaccgc tctgttcgag catgtagata gttacctggt
    60061 ctggcgtgtt tttgccgctc tgataacgcg ccatatgact gttcacgcta atgcgcatac
    60121 tgtccagcac cggcgaatta agaagcacca gtatcaccaa caccaataac gaaaccgcaa
    60181 ggttcacttt gccctgaaga acaaggggat tttgcccatt acgccagacg atactgacaa
    60241 aataccccag cgaccacacc agtaacacca gcaccgccag cgcgccctgc aagcggtcga
    60301 cagtccagcc gtactgagcg acccgcagcc ataacgccca ggcggcgacg aacacataca
    60361 gcggagcaac cagcaaagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagtg
    60421 acgctttttg cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtta
    60481 acagcaaccc ggcggcggag atgtggcgag aaatcgcgct caggtccgta aacggcaggg
    60541 tgatgataaa catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattagcg
    60601 tgaacaactt ttgaatagag tcgattaaac gtgactgtgt acgcgcgagg atcaccacca
    60661 gtgcggtaac cagaccaaat gtaagataca taaaccagtc ggttgcaaaa aagagcgtat
    60721 tgaaaaacgt gatgccaaca agtttaaaca actcacccca cagtaaaagc accagccacg
    60781 tcaggccatt ggagagaaaa atcaccagta atataagtac gttatgccat actgactggt
    60841 aaaagtagct atagcggcta ctgccattgc ggatacgaag gctttgttgt atccacggta
    60901 gcaacaacat tgccatcaac agcagatatc agccaaaatc ccaaatcgcc ttttcagctc
    60961 tccaggggtt catgccatca gtttgccact tcagccaacc gctcatccct gtcgtggcaa
    61021 taaacaccag cgccagccat ccccaaaggc gcttctgttt aaaagagatc acggagaaga
    61081 gtaaaacgga tgaaaacgcg acggtcgcag gcacgccata aacaatccaa ctgtgatttt
    61141 ttccggcaag ccaggcgata agcaggtagc aaaccagccc ctgaagtaat ccggtagcaa
    61201 tcatccccca gcgtgttgcc ggtgaaagtt caacgttatc catgttggtt atccagaatc
    61261 aaaaggtggg ttaattatcg catccgggca gtagtatttt gcttttttca gaaaataatc
    61321 aaaaaaagtt agcgtggtga atcgatactt taccggttga atttgcatca atttcattca
    61381 ggaatgcgat tccactcaca atattcccgc catataaacc aagatttaac cttttgagaa
    61441 cattttccac acctaaaatg ctatttctgc gataatagca accgtttcgt gacaggaatc
    61501 acggagtttt ttgtcaaata tgaatttctc cagatacgta aatctatgag ccttgtcgcg
    61561 gttaacaccc ccaaaaagac tttactattc aggcaataca tattggctaa ggagcagtga
    61621 aatgcgcgtt aacaatggtt tgaccccgca agaactcgag gcttatggta tcagtgacgt
    61681 acatgatatc gtttacaacc caagctacga cctgctgtat caggaagagc tcgatccgag
    61741 cctgacaggt tatgagcgcg gggtgttaac taatctgggt gccgttgccg tcgataccgg
    61801 gatcttcacc ggtcgttcac caaaagataa gtatatcgtc cgtgacgata ccactcgcga
    61861 tactttctgg tgggcagaca aaggcaaagg taagaacgac aacaaacctc tctctccgga
    61921 aacctggcag catctgaaag gcctggtgac caggcagctt tccggcaaac gtctgttcgt
    61981 tgtcgacgct ttctgtggtg cgaacccgga tactcgtctt tccgtccgtt tcatcaccga
    62041 agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct
    62101 ggcaggtttc aaactagact ttatcgttat gaacggcgcg aagtgcacta acccgcagtg
    62161 gaaagaacag ggtctgaact ccgaaaactt cgtggcgttt aacctgaccg agcgcatgca
    62221 gctgattggc ggcacctggt acggcggcga aatgaagaaa gggatgttct cgatgatgaa
    62281 ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg ttggcgagaa
    62341 aggcgatgtt gcggtgttct tcggcctttc cggcaccggt aaaaccaccc tttccaccga
    62401 cccgaaacgc cgcctgattg gcgatgacga acacggctgg gacgatgacg gcgtgtttaa
    62461 tttcgaaggc ggctgctacg caaaaactat caagctgtcg aaagaagcgg aacctgaaat
    62521 ctacaacgct atccgtcgtg atgcgctgct ggaaaacgtc accgtgcgtg aagatggcac
    62581 tatcgacttt gatgatggtt caaaaaccga gaacacccgc gtttcttatc caatctatca
    62641 catcgataac atcgttaagc cggtttccaa agcgggccac gcgactaagg ttatcttcct
    62701 gactgctgat gctttcggcg tgttgccgcc ggtttctcgc ctgactgccg atcaaaccca
    62761 gtatcacttc ctctctggct tcaccgccaa actggccggt actgagcgtg gcatcaccga
    62821 accgacgcca accttctccg cttgcttcgg cgcggcgttc ctgtcgctgc acccgactca
    62881 gtacgcagaa gtgctggtga aacgtatgca ggcggcgggc gcgcaggctt atctggttaa
    62941 cactggctgg aacggcactg gcaaacgtat ctcgattaaa gatacccgcg ccattatcga
    63001 cgccatcctc aacggttcgc tggataatgc agaaaccttc actctgccga tgtttaacct
    63061 ggcgatccca accgaactgc cgggcgtaga cacgaagatt ctcgatccgc gtaacaccta
    63121 cgcttctccg gaacagtggc aggaaaaagc cgaaaccctg gcgaaactgt ttatcgacaa
    63181 cttcgataaa tacaccgaca cccctgcggg tgccgcgctg gtagcggctg gtccgaaact
    63241 gtaatgattt gaagctggag aatatctatc cagtatctta tagaaagcaa aacgggaggc
    63301 accttcgcct cccgtttatt tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca
    63361 ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct ggtgccaagc tccagcatcc
    63421 cgttatggtt atccacgata cgctgcacaa ttgccagccc taatcccgtg ccgctaatgg
    63481 tgcgcgcact gtcgccgcgg acaaacggct ggaacaggtg cttacgttgt tccggcgcaa
    63541 ttcccggacc gtcatcttcc acctggaacc aggcgcgatt cggctccgtt ccgctgctga
    63601 ctttgatcca gccattgcca taacgagcgg cgttgaccac catattcgcc accgcgcgtt
    63661 tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg gtaaagcgcg gtttcaattt
    63721 cccgctcata gccactttcg gcagcaatca cctcaccgag cactgcatta agatccgcca
    63781 tttccatcgg catctcctgc ccggtgcgca ggtagtcgat aaactgctca atgatggcgt
    63841 tgcactcttc gatatcttta ttgatcgatt ctgccagata gccatcctgc tcgctcatca
    63901 tctcagtcgc caggcgaata cgcgtcagcg gcgtgcgcaa gtcgtgactt acccccgcca
    63961 tcagcagcgt gcggtcatcc gccagttgct taacaccagc cgccatatgg ttaaaggcgc
    64021 gggtaacgga acgcacctcg gacgcgccat actcacgcag cggcggcgga ataatccctt
    64081 taccgacctg caaagcagca tgttcgagat cgaccaacgg tcggttctgg atacgaataa
    64141 acagccacgc cccgcctatc gccaatagca taatcgccag cgtatagcgg aacagcggag
    64201 agaaatcgcc ctgatgaatt tcggtcagcg gcacgcgtac ccagatattg ggcgacagcc
    64261 aggttttcag ccagacgaca ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc
    64321 cgcccagttg ctgtgccatc tgatggctta agaattcata gtgttgcgcc caacgcagac
    64381 ctgcctcttc ggcagcctcg ttggagtaga gagagatccc cagctcacgg tagatctccc
    64441 gacggaaagc gggaggcaca accaactgcg tgccgtcctc cagttgcagt ttgtcggtca
    64501 tcaacatacg cacttcgtac gcgaggactt tattaaactg ctggaggctc ggcaaaatcg
    64561 cgaagttcag caccaccaga taagtcgtca ccaggctggc gaacagcaag gtgacgatga
    64621 gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg caatcgcctc atgctttaga
    64681 gccgtccggt acaaagacgt agcccaggcc ccaaacggtc tgaatgtaac gcggatgcgc
    64741 tggatcttct tctaccatgc ggcgcagacg cgaaatctgc acgtcgatgg agcgttccat
    64801 tgcggaatat tcacgaccac gggcaaggtt catcagctta tcgcgggaga gcggctcacg
    64861 cggatggctg accagtgcct tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc
    64921 atcttcgcgg aacatttcgc gcgtaccgag gttaagtttg aacttaccga aagcaattac
    64981 cgcctcttcc tgtgacggtg cgcctggcag ttcgttcgcc tgacgacgca gcaccgcacg
    65041 aatacgggcc agcagttcac gcgggttaaa cggttttgga atgtagtcgt cagcgccaat
    65101 ctccaggcct acgatacggt ccacttcttc ccctttcgcc gtcaccataa tgatcggcat
    65161 cgggttgctc tgactacgaa gacgtcggca aatcgacaag ccatcttcac caggtaacat
    65221 taaatccagt accataagat ggaaagattc acgagtcagc aggcgatcca tctgttctgc
    65281 attagcgacg cttcgaacct ggaagccttg ttcggtgaga taacgttcca gcagcgcacg
    65341 caggcgcatg tcgtcatcga ccaccagaat cttgtagttc tcttgcattg tttgtactcc
    65401 caaaggttcg caacaatttg taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc
    65461 taaatctggt atgaatttca gcctaaattg ttacaaagca tattaaacag cagcttaagt
    65521 atacaattta ttcggcgaaa cattattgat tctgttgata tgatcacgtt atacccaatg
    65581 tgcgcattat caaacagaca aagggaatca acgagatgaa aacgcccctg gttacccggg
    65641 aagggtatga aaaactcaaa caagagctta attatctctg gcgtgaagaa cgcccggagg
    65701 tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc
    65761 agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt gcgctatctc actaaatgca
    65821 tggaaaatct caaaatcgtc aattactccc ctcagcagga aggcaaagtc ttttttggcg
    65881 cgtgggtgga gattgaaaac gacgatggcg tgactcaccg tttccgtatt gtcggctacg
    65941 atgaaatttt tggccgtaaa gattacatct ctatcgattc cccgatggcc cgcgcattgc
    66001 tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc tgccggggaa gcgagctggt
    66061 atgttaatgc tatcgaatac gtgaaaccgt aatgaagagt cttaacctcc tgccgatggc
    66121 tggcattttt gccagccagt ccgtataact atcccctgat ttttgatccg aaaagatgaa
    66181 ctcaaaccat gatgaatgat tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc
    66241 cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg gaataccgtg ccgtttatcg
    66301 cacgttatcg taaggaaatc accggcggtc tggatgacac gcagctgcgt aatctggaaa
    66361 cgcgtctgag ctatctgcgc gagctggaag agagacgtca ggcgatcctc aagtccattt
    66421 ccgagcaagg caaactcacc gatgatctgg cgaaggccat caacgccacc ctaagcaaaa
    66481 ccgaactcga agacctctac ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg
    66541 ccattgaagc agggcttgag ccgttggctg acctgctgtg gagcgatccg tcacacacac
    66601 cagaagtcgc cgctgcgcaa tatattgatg ccgataaagg cgtggcagat accaaagccg
    66661 cgctggacgg cgcgcgctat atcctgatgg aacggtttgc cgaagatgcc gcgctgctgg
    66721 cgaaagtgcg tgattatctg tggaagaacg cgcatttggt ttctacggtg gtgagcggta
    66781 aagaagagga aggggcgaaa ttccgcgact atttcgatca tcacgaaccg ttgtccacgg
    66841 tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa cgaaggcgta ctccagcttt
    66901 cgctgaatgc cgatccgcag ttcgatgagc cgcccaaaga gagctattgc gagcaaatca
    66961 tcatggatca ccttggcctg cgcctgaaca atgccccggc ggatagctgg cgcaaaggcg
    67021 tagtgagctg gacctggcgc atcaaggtgc tgatgcatct ggaaaccgaa ctgatgggta
    67081 ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc
    67141 tgctgatggc ggcccctgcc ggactgcgtg caacgatggg cctcgatccg ggtctgcgta
    67201 ccggggtaaa agtggcagtg gtcgatgcca ctggcaaact ggtggcgacc gataccattt
    67261 acccgcacac cggacaagcc gcaaaagtag cgatgaccgt tgctgccttg tgtgaaaaac
    67321 ataacgttga actggtggcg atcggcaacg gtacagcttc ccgcgaaact gaacgtttct
    67381 atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca gaaagtgatc gtcagcgaag
    67441 ctggcgcgtc ggtttactca gcttccgagc tggcagcgca ggagttcccg gatctcgacg
    67501 tttcgctgcg tggtgcggta tctatcgccc gccgtttgca ggatccgctg gcggagctgg
    67561 tgaaaatcga tccgaaatct atcggcgtag gtcagtatca gcatgacgtc agccagacgc
    67621 aactggcccg caaactggat gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc
    67681 tcaacaccgc ttctgttccg ctattaactc gcgtggcggg cctgacgcgc atgatggcgc
    67741 aaaacatcgt ggcctggcgc gatgagaacg gccagttcaa gaaccgtcag caactgctga
    67801 aagtcagccg tctggggccg aaagccttcg agcagtgcgc gggcttcctg cgcattaacc
    67861 acggtgataa cccgctggac gcctctaccg ttcacccgga agcctatccg gtggtggaac
    67921 gcattctggc agcaacacag caggcactga aagatctgat gggtaacagc agcgaactgc
    67981 gtaacctgaa agcgtctgac tttactgatg aaaaattcgg tgtgccgaca gtaactgaca
    68041 tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt
    68101 ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg tatgatcctc gaaggcgctg
    68161 tcaccaacgt caccaacttt ggcgcgtttg tcgatattgg cgtgcatcag gacggcctgg
    68221 ttcacatctc ttcattgtcg aacaagtttg tggaagatcc gcataccgtg gtgaaagcgg
    68281 gcgacattgt gaaggtgaaa gtgctggaag tggatcttca gcgtaaacgt atcgccctga
    68341 ccatgcgtct ggacgagcag cctggcgaaa ccaacgcccg tcgcggcggc ggtaatgaac
    68401 gcccgcaaaa caaccgcccg gcagccaaac cacgcggtcg tgaagcgcag cctgccggta
    68461 atagcgcgat gatggatgcg ctggcggcgg caatgggcaa aaaacgttaa acgcccggac
    68521 tggcccctaa acctgcgcga taaggcgttc acgccgcatc tggcaacgaa cgccttgcct
    68581 gacataaaag tgccggagaa tatctccggc ctttttattc cacaaccaaa ctcataatat
    68641 attccggcaa tatttatcac ttcattaaca actgaaacct taattaaaca ttagccagtc
    68701 cgggtaattc actattcgaa ttatattttc gctgcgatat aaccttgagc cacatcaaca
    68761 ttgagtcaga ttattattca aaccaacatt cgcacacatt ttaagtattg ctgatagaaa
    68821 ccattctcat tatcattgtg ttgttgatta tttaatctct cctttgttgg caaatcatct
    68881 ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc cagttaatga gaaacaagta
    68941 ggcacctatg caatacactc cagatactgc gtggaaaatc actggctttt cccgtgaaat
    69001 cagcccggca tatcgccaaa aactgctttc tcttggcatg ttacctggct cctcttttaa
    69061 tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct
    69121 ggtattacgc aaaaaagatc tggccttatt agaagtggaa gcggtttcct gttaatacgg
    69181 tgataacaac aatgaaaaaa ttaaccattg gcttaattgg taatccaaat tctggcaaga
    69241 caaccttatt taaccagctc actagcgcac gtcagcgtgt aggtaactgg gctggcgtta
    69301 ccgtcgaacg taaagaaggg caattctcca ccaccgatca tcaggtcacg ctggtggacc
    69361 tgcccggcac ctattctctg accaccatct catcgcagac ctcgctcgat gagcaaatcg
    69421 cctgtcacta cattttgagt ggcgacgccg acctgctgat taacgtggtg gatgcgtcta
    69481 accttgagcg taacctgtac ctgacgctac aactgctgga actcggcatt ccctgcatcg
    69541 tggcactgaa catgctcgac attgccgaga agcaaaatat tcgtattgaa attgatgctc
    69601 tgtcggcgcg tcttggctgc ccagtgatcc cgctggtttc aacccgtggt cgcggtattg
    69661 aagcgctcaa gctggcgatt gatcgctata aagctaacga gaatgtggaa ctggtgcatt
    69721 acgcacagcc gctactcaac gaagcagatt cactggcaaa agtgatgcct tccgacatcc
    69781 cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga aggcgatatc tacagccgcg
    69841 cctacgccgg tgaagcgtcg cagcatctgg atgccgccct tgcccgtctg cgtaatgaaa
    69901 tggatgatcc ggcgctgcac attgccgatg cgcgttacca gtgcatagct gccatctgtg
    69961 atgtggtaag caacaccctg acggcagaac ccagccgttt caccactgcg gtagataaaa
    70021 tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc
    70081 tgctggctat caacatcggc ggggcgttac agccgctgtt tgacgtcggc tccgtggcgc
    70141 tatttgtgca tggtattcaa tggattggct acacgctcca cttcccggac tggctgacta
    70201 tcttcctcgc ccagggcctg ggtggcggta ttaacaccgt gctgccactg gtgccgcaga
    70261 tcggcatgat gtacctgttc ctctccttcc ttgaggactc cggctatatg gcgcgtgcag
    70321 cgtttgtgat ggaccgcctg atgcaggcgc tgggcttgcc ggggaaatcc tttgtgccgc
    70381 tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg cgcacgtacg cttgatgcac
    70441 cgcgtgaacg tctgatgacc atcatgatgg caccgtttat gtcctgcggc gcgcgtctgg
    70501 ctatcttcgc agtattcgcg gctgccttct tcgggcagaa cggtgccctg gcggttttct
    70561 cgctgtatat gctcggtatc gtgatggcgg tgctgactgg cctgatgctc aagtacacca
    70621 tcatgcgcgg tgaagcgacg ccgtttgtca tggagctgcc ggtctaccat gtaccacacg
    70681 ttaaaagcct gattatccag acctggcagc gtctgaaggg cttcgttctg cgtgctggta
    70741 aagtgattat catcgtcagc attttcctga gcgctttcaa cagcttctcg ttgagcggga
    70801 aaatcgtcga taacatcaac gactcggcgc tggcgtccgt cagccgggtg ataacccctg
    70861 tcttcaagcc gattggcgta catgaagata actggcaggc aacggttggc ctgtttacgg
    70921 gcgcaatggc aaaagaagtg gtggtgggta cgctcaacac cctctacacc gcagaaaata
    70981 ttcaggacga agagttcaat ccggcggaat tcaacctcgg tgaagagctg ttcagtgcgg
    71041 tagatgaaac ctggcagagc ctgaaagaca ccttcagcct tagcgtactg atgaacccca
    71101 ttgaagctag caaaggcgac ggtgaaatgg gtaccggggc gatgggcgtg atggatcaga
    71161 aattcggtag cgcagctgcc gcatacagct acctgatttt cgtcctgctg tatgtaccat
    71221 gtatctcggt gatgggagcc atcgcccgtg aatcaagccg tggctggatg ggcttctcca
    71281 tcctgtgggg gctgaatatc gcttactcac tggcaacatt gttctatcag gtcgccagct
    71341 acagtcagca cccaacttac agcctggtgt gcattctggc ggtaatcctg tttaacatcg
    71401 tagttatcgg tctgctgcgc cgcgcgcgta gccgggtgga tatcgaactg ctggcaaccc
    71461 gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg tgattgccat taatggcttc
    71521 acttattcag gtacgtgatt tgctggcgtt acggggccgt atggaagcga cccagataag
    71581 ccagacattg aacactccgc agccaatgat taacgccatg ctgcaacaac tggaaagtat
    71641 gggcaaagcc gtgcggattc aggaagaacc tgacggctgc ctctctggca gttgtaaaag
    71701 ctgcccggaa ggaaaagcct gtctgcgcga gtggtgggcg ctgcgttaac cttactccat
    71761 cgcctgtttt ggaaagcccg gtatgcgtct gcatccgggc ttttttgcgt gcggctttcc
    71821 ataaaaatgc aactcttgca gcacggcgta agttcctttg aaagcatctc gcagggatga
    71881 aaactcgcta atgcacaggt gtggagtggc gcgtagagtc gcggcattca aacaacaggt
    71941 gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgctg ttcaaactct
    72001 ttttacgcca accggacacg gctcgtgatt ttcttgcgtt tcatttaccg gcacccattc
    72061 acgcgctttg tgatatgaaa accctcaagc tggagtcgag cagctttatt gatgacgatc
    72121 tgcgtgaaag ctattccgat gtgctgtggt cggtgaaaac ggaacaagga ccaggataca
    72181 tctattgtct gattgaacat caaagcacct caaacaaact gatcgcattt cgcatgatgc
    72241 gttacgctat tgccgcaatg caaaatcacc ttgatgctgg atacaaaacg ttgccgatgg
    72301 tggtgctatt gttgttttac cacggtattg aaagccccta tccctattcg ctgtgttggc
    72361 tggattgttt cgccgatccc aaactggcaa ggcagcttta tgcctccgca tttccgctga
    72421 ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca ccgacgtatg gcgctgctgg
    72481 agttaattca aaaacatatt cgtcaacgcg
//
LOCUS       DAQSWQ010000007        70304 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.007, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000007 DAQSWQ010000000
VERSION     DAQSWQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.007"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..675)
                     /locus_tag="R8G18_000470"
     CDS             complement(<1..675)
                     /locus_tag="R8G18_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HEE9794266.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHF"
     gene            complement(697..2709)
                     /gene="hyfR"
                     /locus_tag="R8G18_000471"
     CDS             complement(697..2709)
                     /gene="hyfR"
                     /locus_tag="R8G18_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HEE9794267.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2739..3152)
                     /gene="hyfJ"
                     /locus_tag="R8G18_000472"
     CDS             complement(2739..3152)
                     /gene="hyfJ"
                     /locus_tag="R8G18_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HEE9794268.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3145..3903)
                     /gene="hyfI"
                     /locus_tag="R8G18_000473"
     CDS             complement(3145..3903)
                     /gene="hyfI"
                     /locus_tag="R8G18_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HEE9794269.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3900..4439)
                     /gene="hyfH"
                     /locus_tag="R8G18_000474"
     CDS             complement(3900..4439)
                     /gene="hyfH"
                     /locus_tag="R8G18_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HEE9794270.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4449..6164)
                     /gene="hyfG"
                     /locus_tag="R8G18_000475"
     CDS             complement(4449..6164)
                     /gene="hyfG"
                     /locus_tag="R8G18_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HEE9794271.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6154..7734)
                     /gene="hyfF"
                     /locus_tag="R8G18_000476"
     CDS             complement(6154..7734)
                     /gene="hyfF"
                     /locus_tag="R8G18_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEE9794272.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7739..8389)
                     /gene="hyfE"
                     /locus_tag="R8G18_000477"
     CDS             complement(7739..8389)
                     /gene="hyfE"
                     /locus_tag="R8G18_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HEE9794273.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8401..9753)
                     /gene="hyfD"
                     /locus_tag="R8G18_000478"
     CDS             complement(8401..9753)
                     /gene="hyfD"
                     /locus_tag="R8G18_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HEE9794274.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9729..9839)
                     /locus_tag="R8G18_000479"
     CDS             complement(9729..9839)
                     /locus_tag="R8G18_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HEE9794275.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9856..10803)
                     /gene="hyfC"
                     /locus_tag="R8G18_000480"
     CDS             complement(9856..10803)
                     /gene="hyfC"
                     /locus_tag="R8G18_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HEE9794276.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10814..12832)
                     /gene="hyfB"
                     /locus_tag="R8G18_000481"
     CDS             complement(10814..12832)
                     /gene="hyfB"
                     /locus_tag="R8G18_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEE9794277.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12832..13449)
                     /gene="hyfA"
                     /locus_tag="R8G18_000482"
     CDS             complement(12832..13449)
                     /gene="hyfA"
                     /locus_tag="R8G18_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HEE9794278.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13702..14172)
                     /gene="bcp"
                     /locus_tag="R8G18_000483"
     CDS             complement(13702..14172)
                     /gene="bcp"
                     /locus_tag="R8G18_000483"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEE9794279.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14172..14744)
                     /locus_tag="R8G18_000484"
     CDS             complement(14172..14744)
                     /locus_tag="R8G18_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HEE9794280.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14890..15768
                     /gene="dapA"
                     /locus_tag="R8G18_000485"
     CDS             14890..15768
                     /gene="dapA"
                     /locus_tag="R8G18_000485"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEE9794281.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15785..16819
                     /gene="bamC"
                     /locus_tag="R8G18_000486"
     CDS             15785..16819
                     /gene="bamC"
                     /locus_tag="R8G18_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEE9794282.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17032..17745
                     /gene="purC"
                     /locus_tag="R8G18_000487"
     CDS             17032..17745
                     /gene="purC"
                     /locus_tag="R8G18_000487"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEE9794283.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17913..18761
                     /locus_tag="R8G18_000488"
     CDS             17913..18761
                     /locus_tag="R8G18_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEE9794284.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18776..20791
                     /gene="tmcA"
                     /locus_tag="R8G18_000489"
     CDS             18776..20791
                     /gene="tmcA"
                     /locus_tag="R8G18_000489"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HEE9794285.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20865..21563
                     /gene="ypfH"
                     /locus_tag="R8G18_000490"
     CDS             20865..21563
                     /gene="ypfH"
                     /locus_tag="R8G18_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEE9794286.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21673..21873)
                     /locus_tag="R8G18_000491"
     CDS             complement(21673..21873)
                     /locus_tag="R8G18_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HEE9794287.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21901..23028)
                     /gene="dapE"
                     /locus_tag="R8G18_000492"
     CDS             complement(21901..23028)
                     /gene="dapE"
                     /locus_tag="R8G18_000492"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEE9794288.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23032..23388)
                     /locus_tag="R8G18_000493"
     CDS             complement(23032..23388)
                     /locus_tag="R8G18_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEE9794289.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23770..23829
                     /gene="ypfM"
                     /locus_tag="R8G18_000494"
     CDS             23770..23829
                     /gene="ypfM"
                     /locus_tag="R8G18_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HEE9794290.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23928..27041)
                     /gene="acrD"
                     /locus_tag="R8G18_000495"
     CDS             complement(23928..27041)
                     /gene="acrD"
                     /locus_tag="R8G18_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HEE9794291.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27205..28905)
                     /gene="narQ"
                     /locus_tag="R8G18_000496"
     CDS             complement(27205..28905)
                     /gene="narQ"
                     /locus_tag="R8G18_000496"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HEE9794292.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29111..31090
                     /gene="aegA"
                     /locus_tag="R8G18_000497"
     CDS             29111..31090
                     /gene="aegA"
                     /locus_tag="R8G18_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HEE9794293.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31158..31733
                     /gene="nudK"
                     /locus_tag="R8G18_000498"
                     /pseudo
     CDS             31158..31733
                     /gene="nudK"
                     /locus_tag="R8G18_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            31859..32902
                     /locus_tag="R8G18_000499"
     CDS             31859..32902
                     /locus_tag="R8G18_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEE9794294.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32998..35001)
                     /gene="tkt"
                     /locus_tag="R8G18_000500"
     CDS             complement(32998..35001)
                     /gene="tkt"
                     /locus_tag="R8G18_000500"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEE9794295.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35021..35971)
                     /gene="tal"
                     /locus_tag="R8G18_000501"
     CDS             complement(35021..35971)
                     /gene="tal"
                     /locus_tag="R8G18_000501"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEE9794296.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36260..38539
                     /gene="maeB"
                     /locus_tag="R8G18_000502"
     CDS             36260..38539
                     /gene="maeB"
                     /locus_tag="R8G18_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HEE9794297.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38833..39168
                     /gene="eutS"
                     /locus_tag="R8G18_000503"
     CDS             38833..39168
                     /gene="eutS"
                     /locus_tag="R8G18_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HEE9794298.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39181..39660
                     /gene="eutP"
                     /locus_tag="R8G18_000504"
     CDS             39181..39660
                     /gene="eutP"
                     /locus_tag="R8G18_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HEE9794299.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39635..40336
                     /gene="eutQ"
                     /locus_tag="R8G18_000505"
     CDS             39635..40336
                     /gene="eutQ"
                     /locus_tag="R8G18_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HEE9794300.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40333..41136
                     /gene="eutT"
                     /locus_tag="R8G18_000506"
     CDS             40333..41136
                     /gene="eutT"
                     /locus_tag="R8G18_000506"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HEE9794301.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41133..42149
                     /gene="pta"
                     /locus_tag="R8G18_000507"
     CDS             41133..42149
                     /gene="pta"
                     /locus_tag="R8G18_000507"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEE9794302.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42188..42481
                     /gene="eutM"
                     /locus_tag="R8G18_000508"
     CDS             42188..42481
                     /gene="eutM"
                     /locus_tag="R8G18_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HEE9794303.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42588..42875
                     /gene="eutN"
                     /locus_tag="R8G18_000509"
     CDS             42588..42875
                     /gene="eutN"
                     /locus_tag="R8G18_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HEE9794304.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42887..44290
                     /locus_tag="R8G18_000510"
     CDS             42887..44290
                     /locus_tag="R8G18_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HEE9794305.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44301..45137
                     /gene="eutJ"
                     /locus_tag="R8G18_000511"
     CDS             44301..45137
                     /gene="eutJ"
                     /locus_tag="R8G18_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HEE9794306.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45127..46314
                     /gene="eutG"
                     /locus_tag="R8G18_000512"
     CDS             45127..46314
                     /gene="eutG"
                     /locus_tag="R8G18_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HEE9794307.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46631..47857
                     /gene="eutH"
                     /locus_tag="R8G18_000513"
     CDS             46631..47857
                     /gene="eutH"
                     /locus_tag="R8G18_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HEE9794308.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47854..49257
                     /gene="eutA"
                     /locus_tag="R8G18_000514"
     CDS             47854..49257
                     /gene="eutA"
                     /locus_tag="R8G18_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEE9794309.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49269..50630
                     /gene="eutB"
                     /locus_tag="R8G18_000515"
     CDS             49269..50630
                     /gene="eutB"
                     /locus_tag="R8G18_000515"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HEE9794310.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            50651..51538
                     /gene="eutC"
                     /locus_tag="R8G18_000516"
     CDS             50651..51538
                     /gene="eutC"
                     /locus_tag="R8G18_000516"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HEE9794311.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRIATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51548..52207
                     /gene="eutL"
                     /locus_tag="R8G18_000517"
     CDS             51548..52207
                     /gene="eutL"
                     /locus_tag="R8G18_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HEE9794312.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52220..52720
                     /gene="eutK"
                     /locus_tag="R8G18_000518"
     CDS             52220..52720
                     /gene="eutK"
                     /locus_tag="R8G18_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HEE9794313.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52766..53818
                     /gene="eutR"
                     /locus_tag="R8G18_000519"
     CDS             52766..53818
                     /gene="eutR"
                     /locus_tag="R8G18_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HEE9794314.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53824..54723)
                     /gene="hemF"
                     /locus_tag="R8G18_000520"
     CDS             complement(53824..54723)
                     /gene="hemF"
                     /locus_tag="R8G18_000520"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEE9794315.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54727..55596)
                     /gene="amiA"
                     /locus_tag="R8G18_000521"
     CDS             complement(54727..55596)
                     /gene="amiA"
                     /locus_tag="R8G18_000521"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HEE9794316.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55810..56235
                     /locus_tag="R8G18_000522"
     CDS             55810..56235
                     /locus_tag="R8G18_000522"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEE9794317.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56222..56671
                     /locus_tag="R8G18_000523"
     CDS             56222..56671
                     /locus_tag="R8G18_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HEE9794318.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56732..57307
                     /locus_tag="R8G18_000524"
     CDS             56732..57307
                     /locus_tag="R8G18_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HEE9794319.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57403..58302
                     /gene="yfeX"
                     /locus_tag="R8G18_000525"
     CDS             57403..58302
                     /gene="yfeX"
                     /locus_tag="R8G18_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HEE9794320.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58360..59664)
                     /gene="pbp4b"
                     /locus_tag="R8G18_000526"
     CDS             complement(58360..59664)
                     /gene="pbp4b"
                     /locus_tag="R8G18_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HEE9794321.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59669..59842)
                     /locus_tag="R8G18_000527"
     CDS             complement(59669..59842)
                     /locus_tag="R8G18_000527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HEE9794322.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59855..61093)
                     /gene="murP"
                     /locus_tag="R8G18_000528"
     CDS             complement(59855..61093)
                     /gene="murP"
                     /locus_tag="R8G18_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HEE9794323.1"
                     /translation="MAKEISSELLNTILTRVGGLGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMSDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIA"
     gene            complement(61097..61993)
                     /gene="murQ"
                     /locus_tag="R8G18_000529"
     CDS             complement(61097..61993)
                     /gene="murQ"
                     /locus_tag="R8G18_000529"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEE9794324.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62157..63014
                     /gene="murR"
                     /locus_tag="R8G18_000530"
     CDS             62157..63014
                     /gene="murR"
                     /locus_tag="R8G18_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HEE9794325.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63143..63934
                     /gene="ucpA"
                     /locus_tag="R8G18_000531"
     CDS             63143..63934
                     /gene="ucpA"
                     /locus_tag="R8G18_000531"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HEE9794326.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64105..65121
                     /gene="cysP"
                     /locus_tag="R8G18_000532"
     CDS             64105..65121
                     /gene="cysP"
                     /locus_tag="R8G18_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HEE9794327.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65121..65954
                     /gene="cysT"
                     /locus_tag="R8G18_000533"
     CDS             65121..65954
                     /gene="cysT"
                     /locus_tag="R8G18_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HEE9794328.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65954..66829
                     /gene="cysW"
                     /locus_tag="R8G18_000534"
     CDS             65954..66829
                     /gene="cysW"
                     /locus_tag="R8G18_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HEE9794329.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66819..67916
                     /gene="cysA"
                     /locus_tag="R8G18_000535"
     CDS             66819..67916
                     /gene="cysA"
                     /locus_tag="R8G18_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HEE9794330.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68050..68961
                     /gene="cysM"
                     /locus_tag="R8G18_000536"
     CDS             68050..68961
                     /gene="cysM"
                     /locus_tag="R8G18_000536"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HEE9794331.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68964..69332)
                     /locus_tag="R8G18_000537"
     CDS             complement(68964..69332)
                     /locus_tag="R8G18_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HEE9794332.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69437..70288
                     /gene="pdxK"
                     /locus_tag="R8G18_000538"
                     /gene_synonym="thiJ"
     CDS             69437..70288
                     /gene="pdxK"
                     /locus_tag="R8G18_000538"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HEE9794333.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16702 a        18357 c        19435 g        15810 t
ORIGIN      
        1 gaaatggcga atggcaatgg cgaacggaat cacaaacaaa ttggcgatac agtgctcaaa
       61 cccactggcg acaaacaggg tgatgggcaa aatcatggcg acgattttgt cgcataatga
      121 acgcccgcaa taactcatcc acagcgccag acaaaccatc agattgcaca taatgccgag
      181 gctgacagat tcagtaaatg tatgatgcat tttgccctcg gcgcagtgta aaaccgcgac
      241 tccccacatc gcgttttcac tcatcaccag cccggaaaac cagattaaca aactgaaaca
      301 ggcaatacct gccagattac cgcaggccac cagaagtgcg ttaatcagcc aagttcgcca
      361 actaataacg ccccgacttt ttgccatcac cgtcattacc gacgaggtga acaggctggt
      421 gccacaaacc gccagcaaaa taaagccgag tgtaaagcaa aggccgccca ccagatgggt
      481 taatgcctgt gaagacgggg catcggcgat aacggaaagg taaaaagtaa atccaatcgc
      541 cataaacacg ccagcggaca tcgccattaa aaagaccgac actttgctgc gggcaatttt
      601 atgggcagta atccgttcag cgattgccgc ttttctggcg ctcaactgca agtcgaaaga
      661 gagtttgttt ctcatgcata ctcctgcaaa agcagattac aacacctcgc gaaccgagag
      721 ccccagacgc tgcattcgtg acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc
      781 gccacggggc ccggcaacaa tgccattggt ttctcgcaat acctgaacaa tgcgctgacg
      841 ttcttcttcg tcattctctg gcgtcgtcgg gtgcagcaac tgcgccattg aactgcgaag
      901 cgctgaatct tcatttagcg tcggcagtaa atggctttgt cggacattaa gatgtaaatt
      961 cagactgtta ccgcgagtca gcagtaccgc tcgctcaatc acgttttcca gctcgcgcac
     1021 gttgcccggc cagtcccacg acatcaactg gcgtagcgcc tcggtcggga tggcgtcaat
     1081 tgaacgattc atatggcgcg ccattttttg cgtgaaatgc tttgctaaaa gagggatatc
     1141 ttccggacgg tcgcgtagcg gcggcaattc cagtgggaag acattcaggc gataaaagag
     1201 atcgctgcga aactggcgat cttcaaccat ttgccacaaa tcacggttgg tggcggcaat
     1261 gactctgaca ttcaccggga tcgttctact cccgccgaga cgctcaatct cccgctcctg
     1321 caatacgcgc agcagtttag gctgaagttc taacggcaga tcgccaattt catcaagaaa
     1381 caacgtgccg ccatcggcaa tttcaaaacg accacgatgg gtattaatcg caccagtaaa
     1441 cgcccctttg tcatgaccga ataactcact ttccagaaga ctggcgggga tggcagcgca
     1501 gttgattttg accagcggct tgtcgcgtcg cgggctaagt tgatggatcg ctctggcgat
     1561 cacctctttg ccggttccgg tttcaccaca aatcaacact gtactgtcgc tcttcgccac
     1621 aatatctacc tgctggagca ggtcttccat tgcctggctt tgatagataa tgtcaccgac
     1681 gcccagattc gaaaggagct gctcgctaag ctggtggttt tcttgctgca aactttcctg
     1741 caaatcggtc atgctacgcc aggcatcggc attgccaacg gcaatagcga tgcgatcggc
     1801 tatgtgttgt agtagctgac agttttcctc actaaagaga gtggaagagg tatgcgccag
     1861 caacaatgca cccggtgtat ggttgccaaa ggtaagcggt ataaggagcg cagattcgca
     1921 gccgttagat gcaagtaaga gtaataacgg gtcgcgttgc cagagaaaca gatcgtctgc
     1981 acggtgcgtc aaggtcggtt gtcgggtttg tagcgtttgt gtcagcaata cactgtcgcc
     2041 aggcatattg cgtggcagac acgcacaatg tgaggcagaa agatcgctgc accacaggct
     2101 aaacttctcg ttctttcgat gatcgcccag taccatactg actgaagcca gaccgaaaaa
     2161 atgatggatc tcacgagcga cgtcagcgat cagatcgtcg agatcaagat gtgacagcac
     2221 tgcattagta atatcaacca gtacccgatg atgatcgcga tctttctcca gcaaacgcag
     2281 cgccacctgc tgttcttcat cattgagcca tgattgcaga accggggtga ttatccgcgc
     2341 cagccagtta aacagtgtgc aatcctgggg agtaaaacgg cagccgtggg tgcgaataaa
     2401 gcgtagctcc gccattccac tactgccgag aaggacggcg tgacgcgaat aatcgaccat
     2461 ctcgccctga tactctgttg ttgccgtcac atcgctgcca tcatcatggt agcggtaata
     2521 gaagttgctt ttgagaacat gcagttcaat aagttgtacc gggctgaacg gcggcggcag
     2581 cgggatgaag gcgcgtaata aagacgcctt accgtgtttc tgcgctaacc gctccgcgag
     2641 cattccgttt accgcttcaa ttgttattcc ttgcggcggg gcaaacatcg cctcgtctga
     2701 catagccatc aacagtcctt attaatgaga aatccttctc acgcaatttt tctggccatc
     2761 agatagatgg ccggttcctg cacaatttca tccaacagat gaatgaattg aatgctacgc
     2821 gcctgccaca cagacgtcgc gtcatccgcc agtggcgcga atgcccgggt caacagggcg
     2881 gtgtggctgg cgtcaataac aatctcacca aaagtcatca cccccagcat cttacgtcgg
     2941 gcttgctcct cttcgaccag tgcaagccaa tcttcgtatt ccgtcagtgg gcaggggaag
     3001 gcgacattca gacaatcaat cacgccaacg tgatgaccga tagccagcga gtaatacatt
     3061 acctgagagc tgtgttccgg catctcgtcg ctactggcga caaacttttt tcgcagcgtc
     3121 cagaaaacaa tttccccgca ctcttcagtc atgtaacccc cggagtacgc gaaagagttg
     3181 ctgaacgata ctattgagac gtggatcgtc ggcatcgcgc aaccaggtgt taacccgatt
     3241 tcctgtagga tcgtcgctta aatggcgcag gagccgatcg caaatttctc gcccctgacg
     3301 atagcccgcc agccgccgcg cttctcgctc aatggcgata cgctgtgatg gcgggatctg
     3361 cggccacaac ggttgcatag tcaccccggt gggatcgcga taatccaccg cgtgaatctt
     3421 ctgttgcagc aaaccgagcg ccacggcgaa accgtgaatg gtggcggccg gtgttggcgg
     3481 gcagccgggg atccaaacat caatggggac aatggtgtcg ctgccgcccc agacgctgta
     3541 gagatcgtgg aaaataccgc cgccgacacc gcacgcgccg taggaaacac aaattttatg
     3601 atcgggggca gactcatacg cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc
     3661 tgtaaataac aaaatatcgg cgtgacgcgg tgatgaaata accttaatgc caaaacgttc
     3721 tgcgtcgaat actggtgtga tggcggcaaa aatttcgatt tcacaggcgt tgcagccgcc
     3781 gcaatcgacg cgataaacgt aagccgagcg acggatatcc tgtagcaaat gctgcttcat
     3841 cttttgcgtt tgctcgtcca gcgtgatggg ctggctgaca tgttgtgtaa gcactggact
     3901 catagctgct ccttagccac cagcggtaca tctgtatcgt cgttgagcag cgtcgcgcgt
     3961 tgtttgcact ccgggcagac gctcgcttgc gcccataaca tttcgcggtt ttgtggcgca
     4021 ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg ttttttgcgg ggcaaacggg
     4081 cgttcgcaac ggctgcaacg ttgtagatgg aacgtcgcgc gggtatagag atcggcttta
     4141 ttggtgacgg tcagttcaaa gttattggta agctggatgg ctctggtcgg gcacacttct
     4201 tcacaacgtc cgcagtaaat acaacgcctc agatagagct gccaggtgcg cgaattttgc
     4261 tggtcgtcgg tctggatagt cagcgcattt gccggacaag cacaggcgcc gcaggcaata
     4321 cattggctgg gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc cagtggcgcg
     4381 aagggatatt tcaccgtcgc ggttccggcg cgcataatag ttttcagtaa cttcagcatc
     4441 ttctgtcctt acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc gatctcttta
     4501 tacggcacgg tttttgactg gcgcttgcgc acatctacca gcgtcacacg gtcggtacag
     4561 gagtagcagg gatcaaggct accgataatc agcggtgcgt cagaaacggt attgccgcgc
     4621 agcatgtaac gcaacaccgg ccagttggcg taggtggcgg cacggcagcg ccagcggaac
     4681 aatttttggt tatcaccgag catgctccag tgcacgtctt caccgcgtgg cgcttcaaca
     4741 aagcccagcg cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag tggggtatcc
     4801 ggcatgttgt cgagggcaaa ttccagcatt gccagcgaat caaacgtctc tttgacacgg
     4861 accatcacgc gggagaaaac atcgccgccg gtaaaggtaa acagtgtttt tggaatattg
     4921 ccgtagtcgg cgtaggggtg atcaaagcgc aaatcacggg caaaaccact gccgcggatc
     4981 agcggcccta cagggctata atcacgggcg atttgtcggt cgagaatgcc aatgccctga
     5041 gtgcgttgtt ccatattcgg cgtggcaagc agcatctcta ccagctccga cacgtcggcg
     5101 cgcatctcgc gcaccagttt cagcgtttgc agacgttgct ctttgagaat atcgcggcga
     5161 acaccaccaa tcagattcag accgtaggtt ttacgcgacc cggtcagcaa ttccgccatt
     5221 gtcatcgact tttcccgcac gcggaaaaat tgcataaagc cggtatcaaa tccaacaaaa
     5281 tggcaggaga ggccaaggtt gagcaaatga ctgtgcagac gttcgacttc cagcagaatc
     5341 gagcgaatag tatgcgctcg ttgcggcacc tcaatcccca gtgcattttc aaccgagttg
     5401 gtataggcca cactgtgggc aaaaccgcaa atcccacaca cgcgatccga taagaaggtc
     5461 acttcgttgt agcccatccg cgtttctgcc agtttctcca tgccgcgatg gacataaaac
     5521 aggcggtaat cagcatcgac aatttgctcg ccatccacaa acaagcggaa gtgacccggt
     5581 tcatcggagg tgatatgcag cgggccaata gggatcaccc gcgcatcgct attgccctca
     5641 ttgataaacg gatacgtttc ggtatcagtc gtcggttcag ggcgcagtcg ataatccatc
     5701 gcatctttgc gcagcggatg catatcttcc ggccagtcat cgggcaacac caggcgacgc
     5761 tgatccggca ggccaaccgg aatcagcccg tacatatcgc gaatttctcg ctcgccccag
     5821 accgcggcag ggacgcgcgg tgtgactgac ggaaactccc gactgtcggc atcgaccagt
     5881 gccttcacca cgatccagca tttttcggcc ccttccattg aaagggcata ataaaccgcg
     5941 taatgaccgt taagtgtccg ctcgtcgttg ccaaacagga ctggaagcca gccatcatgt
     6001 tgataataaa gataatgtac aacgtcaggc agcagattga ttttcaccgt aatggtgacc
     6061 tgttcaggcg tttgtcgctc ttcatccagc accgcgccgg ggaactgcgt tttcagggcg
     6121 gcgagaatcg cttcgccacg atttgacgat gaattaacgt tcactgtgtt tctccgaaac
     6181 agatgcggtg cctgaaggca aaaaatcatg ccaggtgcta cgttgtgcag gcaggtcgtg
     6241 cgtccctgag aggactatag tggaagcgcc cgccaggatc ctgatgacag gttgtggaat
     6301 atgcgttccc atcgccagca tcatgaccag cagaatcacc attggcgagg tggtcaacca
     6361 gccgagatca ccccggttaa cggcctgcgg cggtttcgcc attaacaccc gcgctgccat
     6421 ccgtaccagg cccgccagca ccagcgttaa cagcaataac agcaggacga taatcagcag
     6481 gtgattacgt gccagtccgg cggtaatggt cataaattcg ctaagaaaaa tgttgaaggg
     6541 cggcatccct gccagcgcca gcgcaccgcc gccaaacagc acggcggtaa atggcatgat
     6601 tttgagcatc ccacagacga cgttgagatc gcgcgtgccg tacttgagca gtacattgcc
     6661 ggaaccgcag aacagcagcg tttttgccag actgtggttt aagatgtgca gcagcgcggc
     6721 aaaaattccc agcggcccgc caatgcctag cgccaccgcg accagcccca tgttctccac
     6781 gctggagtac gccagcagac gcttaatgtc ccgctgtacc agaatgaaaa atgccgccac
     6841 ggcaaccgac aacatgccga agatgagcaa caaccggttg gggaaatcgc tgccgatggc
     6901 ttggcaaata atgatgtaat agcgaatcag caccaacagc gcgcagttca gcaatacggc
     6961 ggagagcagg gcgctgaccg gactcggcgc ttcactgtga gcatccggca gccaggcgtg
     7021 catgggaaat agcccggttt tggtaccaaa gccaattagc acaaacacaa aggccaacag
     7081 cattaatgtt gggtcaagca aggacgattg cttaagaacc tcgctccaga atatcgccat
     7141 ttctgcctgc ggcataacgc tggcggcgtt ggcgtatacc agcacggtac cgaacagacc
     7201 aaaagcgaca ccaacagtac aaataatgat gtacttccat gctgattcca gcgatgaacg
     7261 ctgaccgtaa atgcctacca gaaacgccga gcttaaggtg gtggcttcga tcgccgccca
     7321 catcacaatc aggttattgc tggtaacaac cagcagcatg gtgaacaaaa acagatggaa
     7381 gaaaccgtag taatcgcaca gcgtaacggg tgaaagctcg ccgtgtgcca cttcatgacg
     7441 catgtagcca atcgagtaaa caccggtgag aaagccaatc acaccaagaa tggcgaggaa
     7501 caaaccgccc agaccatcaa tatgaagcca cagtcccgca gcgaatattt ctcctgcatc
     7561 agcggcagtt tggaccaccc agagtgccag aatcagcagc agtgtgatcc ctaagctatg
     7621 taatacagtc accgtacaag tcgcagagag cccccgtttc cggcaggcaa aacagagcag
     7681 cgaaaaaagc agcggcgtga gcaggagtaa agcgaacatc acagaataac tcatctcatt
     7741 atcccttcag cgcggtcaag ttgttcacgt ccagcgtgcc gtgggtacgc catatttttc
     7801 ttgccagtaa caccatcaca atgacggcga agatggcgtc ggtagcgata cctatttcca
     7861 ccagttccgg tgctcgccag gcaagaagcg ccagcaccag atgggagccg ttttccatca
     7921 ggcagtaacc aaaaatttgc cgcaggatat tgcgctggct gacaatgcac agcaggccaa
     7981 gcagaaaatg acctaacgct accgccagcg ccggtttcag cccggtagcc atcggtagct
     8041 tcacgggctg aacgacaaat gcgcaaagca ggacaattaa cgccgcgagc agtgccatca
     8101 ttgccggacc gaataacgct ttttccggga tgttctgggg aatatttcgt gcagcgtaag
     8161 tcatgattaa cggtaccagc agcactttgg tgataaaggc gctggcggac cagatcagca
     8221 gttgctctgc ggcgaacagg cacgagagag tggcgaaaat agacaccagc accagcgact
     8281 ggcaggcgta aaatccgcat gacaggcgat agcttttgac gctaatcaca aacagcgatg
     8341 tcagcatcat cagtcccgcc agattattta cgatcataga accggtcatt catcatctcc
     8401 ttactgcaac caggtcgcgg cgattacgct ggaaatcagc gacatcacaa tcagtacaat
     8461 caacaccagg cgcattgacc ctggcagcgg tgcggcatcg gcgacggcct cgctcggttt
     8521 gcccggcacg acgcgaccaa accagcgaat aaaccaggcg aaactggcga ccgattcaat
     8581 catcagcaga atcatggcgg gcagcaggat ccagtactcc actgacaacg caaaaccgac
     8641 ggcaaacagc gggaatttac tgaagaagcc gttgaacggc ggcacaccgg taatcgccag
     8701 cgctgccacg cagaaaccca cgcttggcaa cggcagggtg tgcaatacgc cacgcagacg
     8761 cggcaacaag cgcgtgccgc agctgtaact cagcgcacct gctacaagga aaaacaggct
     8821 tttagcgaac gcgtggttga cgatgtaggc gatgctaccc tccagcgcca gccgcgagcc
     8881 gaagatggag agcgacaagc cgaagaacat ccagccaagt tgagtgatgg tcgaccaggc
     8941 cagcaaccgc ttcatatcct gctgtggcaa atacatcaga aagccataaa gaatggtgac
     9001 cagcgccatg accatgccaa cgccgccaat cacatgcggg atattgccgc cgtcgataat
     9061 ggcgcgggca aaaatgtaaa cgcccacttt caccatcgat gcggcgtgga gataggcact
     9121 gatcggtgtt ggcgcttcca ttgcatccgg tagccacgct tgcatcggta gctgggccga
     9181 tttcccccac gcggcaaaca ggatgccgcc ataaaccaga taacgtgcgt cgccgtgtaa
     9241 ctcgctcatc gcgctaagcg caaacgttcc ggtctgcaaa aacagcgtgg cggcggcaag
     9301 atacaacccc aacgagccga tatgagtgat aagtaacgct tttagtgctg aaagctgtgc
     9361 tttatcgctc tggtaataac tgatcaacgc ccaggagcag ccgcccgtaa tttcaaaaaa
     9421 caacaactga ccgagcagcg tcgaggagag taccagtccc gccatcgcgc cgataaacac
     9481 cagcaaaaat gcgtaataac gattcgtgcc gttatgcggg tgttcgcgat ttttatccgt
     9541 cagataaccc gtggagtaga tcgtgaccag caaaccgaga aacaccacca caaacagaat
     9601 cagcgtactc atgcgatcaa tgaccaagcc aaacaatgcc acatccccca cgttgaccaa
     9661 cgtcaacgtg acggcaactt tatcggcctg ataaaacgcg gagatcagcg acaacatgca
     9721 cagcgtggtc agtgcggcga acaaaacccc cattcggcgg cccgacgttg tggcgaaaac
     9781 gaaacgacca gtgcgccgat aaaaggcagc attaacgtcg tcagagcaag attttccata
     9841 ttccgtcagt gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct
     9901 gaagccaagc caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc
     9961 tgcgattgag gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt
    10021 cagcaggcag gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa
    10081 cagtgctgcc atcacgacct gtttcagccc cagcccccat ttcgctagcg ccagcccggc
    10141 accggaatat tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa
    10201 gggaattttt cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt
    10261 ggttagcggc gagttccagc ccgttgccag cgtattgctg atcatctcga tatgcgtgga
    10321 acctgctatc agcgccaata ccagcagtga gagaatgagc atgggttcga ccagaatgcc
    10381 gagcgtcaac tcgcgactgg caccgactcc cgcaaacggg cttccggtat ccagccctga
    10441 aagagcaaag aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc
    10501 gcccgcaaaa ggggaaacgg taataaatag tggtagggcc atcgccagca ccagcatgct
    10561 gctgattaat acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcatcttc
    10621 ctggcgttta aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg
    10681 ggagtgcata cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag
    10741 cagaatcacg gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct
    10801 catggtgatt tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat
    10861 acaggcaata gagacgaaag tcgccgcttt gcagatgctg tatttctttg gccagccgtt
    10921 gggtggcgct cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag
    10981 ccctggcggt gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc
    11041 gtagacgaaa tagcgcagaa aagaccacac gcagcggctg catcacgcca ttgcctgacg
    11101 gggccatcgc attttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg
    11161 ctgcgcggcg cgaacgacac cacagccagt aaaggccagg cagcaaaggc atcgccagta
    11221 gtaacagcaa cagtaatgaa ggagtgaccc gtgtatgaaa cgtgccgggt acaagtgcta
    11281 ttccgctggc gacagtgacg ggaggggtat tggtaaacgc atgggcaatg tgcattatct
    11341 tcggtgccag ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca
    11401 tggcgacgat catcgtattt ggcacttcct gtgcctcttc agcgtgtgtg ctgcgcggcg
    11461 caccacagaa agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga
    11521 ctgccagcat taccatagca ataggacccg caagttgtag cgctacggct tccacacgac
    11581 ttagtgagaa tagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa
    11641 tggctgatat cgccaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg
    11701 ccagtgcccc cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcacccgcgc
    11761 cgagaaatag cagccctttg aaaagcgcat ggttgagcag atgaaacagt gcgccgagca
    11821 gtccaaccac ggtgagcagc gggtcgtgca gtgacagacc gaccatcgcc acaccgaccg
    11881 ccagcaaaat aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct
    11941 gttccgccag tgcatacagc acgcccagga gcgcggagat tgcgccgatc gccatcacca
    12001 gaatgcccca ccacagcggc aaacccgttt gcgccagcag atccatcgcc actttcagga
    12061 tgccgaaaat accgattttg accattacgc cagacatcaa cgctgaagcg tgcgatggtg
    12121 cggcagggtg agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa
    12181 aaccgaaaaa ggccagcagg aacaccgccg acgccagccc cggagaaagt gaaagcgtgc
    12241 ggaaactggc aaaatcgagg ctgccgcttt cgcgccccat cagcaagaag gcgatcatta
    12301 tcagcaccga tccggcgtgg gcgataaaaa agtagagcat gccagcgttg atcgacgttt
    12361 tatcctgcct ggcaatgacc agaaaccagg aagacagcga catcatttca aacagcacga
    12421 tgaaccaaaa agcgttgtcc atcaccagca gggcaaccat cgatgcgatg aaaatattca
    12481 taaagaagcc catcgccgcc gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat
    12541 agagagaaca aaccaccacc agcaaggaga taaccagcac cataaatgcc gagaggctat
    12601 ccattcgcag ggttaaatcg gcaaattcaa aaggcgtggc aaaacgggcg actaaagtta
    12661 cgcctgcatg taattgcgta attccgctga tgatgccaat cacgccgcct accagcgatg
    12721 tgatgccgga aagcttaata gccagtctgt ccagaccgag taaaaacagc gaagccagac
    12781 tggcaaacag atagaggatc agcgaccagg ttaataattg cagggcatcc attagcgttg
    12841 ctcctgagtg agggaaaggg gatcctcccc gccattggca aaccagcttg cggcaaggcg
    12901 ctgtttttct ttcatctggc gttgcaggct atcgtcggtg atcagccgta acgcctgatt
    12961 cgggcaagcg cgaacgcagg ccggaccttc tggcaagaaa tcacacaggt cgcatttcac
    13021 cgcgacggtc tggacacccg gttcccagcg cagcaacgca tgttggggcg gcacgttttc
    13081 ttcgccgtct ttgagtgagc cctcagcctg aaaaacatat tgcgcagggg cattcaccgg
    13141 acggcttcct gaagcgctga ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc
    13201 aatacagagg ctttcgttga gttggatcgc atcatccctc tgagagatgg cattgaccgg
    13261 gcagacctgc aggcaagggg cttcctcaca gtgatgacac acgacagggg cagtgagtgt
    13321 tgacgtcttc gccagggcca ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc
    13381 cgaacaggca gcgagacagg tattacatcc tgtacaccac agtggttcgg ccaccacaaa
    13441 gcggttcatg tcggctcctt gaaaatgata tgaaacattg catttcatat tgcataaagg
    13501 atgccattct gattaactca atatattcaa taagttatgt attaactgga ttctgaacgg
    13561 tacaaaaatg atggtcatta acgttcagca aaaaatggat tcgacacccg tgtcgcaggg
    13621 gaaagcgaga atgtgatctt gatcacgaga agtgaggtat gctggccgca agcgcagcca
    13681 gcacggaatg gagcaaagta atcaggcgtg ttctttcagc cagttcagca caacgtcgtg
    13741 gtgattgctg gttttgaaat cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag
    13801 gaagctgatg cgatgaatgc catcgtaggt tttgcccatg aaggactttt caccccagac
    13861 gccgaattgt tcgcacacct ggtggtcctc atcagacagg agcgtaaagt taagcagctc
    13921 tttttccgca aaacgggaga gtttttcggg tttatcggtg ctgataccca gcacatcaac
    13981 gcccgctttt ttcaactcat ccatgttatc gcgtaagccg caggcctgta cggtacagcc
    14041 gggggtcatg gctttcgggt agaaataaac cagaacacgc tgtccctgga agtcggtcaa
    14101 attaacttgt tctccgtctt gatccggcaa gctaaatttc ggtgcgatat caccggcttt
    14161 cagtggattc attacttaac tccatcctgt tcatcatgtt gggaataatt gacgacgtta
    14221 atactgcctt gtgcattgag ttctgtacat agggctttga acgcttgctc aatatttgct
    14281 gcgtccgcag atgcggggct gtgggcggtt atctgaatat gcaactgcgc agcccgttca
    14341 ttttcagcag gttgcgtgcg cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa
    14401 agtgctgtga agcgttcaat taaatgcggg gagtctgcca catcgacctg aacccagaca
    14461 gatgctggca ttggcggacg cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc
    14521 agctcggcac ctttcaacgg taacgttgat tcaatcagag taatggcatt ccatgaaccg
    14581 gaaagcagca tgataaacgt gaactcttct cccagcatcg ccaggcgact gtcttcaata
    14641 ttacagccgc aactactgac atgacgggtg atggtgttca caattccagg gcgatcggca
    14701 cccaatgcag tgatcaccag ataatgttgc gatgacagtg tcaaactggt tattccttta
    14761 aggggtgagt tgttcttaag gaaagcataa aaaaaacatg catacaacaa tcagaacggt
    14821 tctgtctgct tgcttttaat gccataccaa acgtaccatt gagacacttg tttgcacaga
    14881 ggatggccca tgttcacggg aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt
    14941 aatgtctgtc gggctagctt gaaaaaactg attgattatc atgtcgccag cggtacttcg
    15001 gcgatcgttt ctgttggcac cactggcgag tccgctacct taaatcatga cgaacatgct
    15061 gatgtggtga tgatgacgct ggagctggct gatgggcgca ttccggtaat tgccgggacc
    15121 ggcgctaacg ctactgcgga agccattagc ctgacgcagc gcttcaatga cagtggtatc
    15181 gtcggctgcc tgacggtaac cccttactac aatcgtccgt cgcaagaagg tttgtatcag
    15241 catttcaaag ccatcgctga gcatactgac ctgccgcaaa ttctgtataa tgtgccgtcc
    15301 cgtactggct gcgatctgct cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt
    15361 atcggaatca aagaggcaac agggaactta acgcgtgtaa accagatcaa agagctggtt
    15421 tcagatgatt ttgttctgct gagcggcgat gatgcgagcg cgctggactt catgcaatta
    15481 ggcggtcatg gggttatttc cgttacggct aacgtcgcag cgcgtgatat ggcccagatg
    15541 tgcaaactgg cagcagaagg gcattttgcc gaggcacgcg ttattaatca gcgtctgatg
    15601 ccattacaca acaaactatt tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag
    15661 gaactgggtc ttgtggcgac cgatacgctg cgcctgccaa tgacaccaat caccgacagt
    15721 ggtcgtgaga cggtcagagc ggcgcttaag catgccggtt tgctgtaaag tttagggaga
    15781 tttgatggct tactctgttc aaaagtcgcg cctggcaaag gttgcgggtg tttcgcttgt
    15841 tttattactc gctgcctgta gttctgactc acgctataag cgtcaggtca gtggtgatga
    15901 agcctacctg gaagcggcac cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc
    15961 ggtgacctcc ggtgattatg caatcccggt gaccaacggt agtggtgctg tcggtaaggc
    16021 gctggacatt cgtccaccag cccagccgct ggcactggtt tctggcgcgc gtacccagtt
    16081 cacgggcgat accgcttcat tgctggtgga aaatggtcgt ggcaatactc tgtggccgca
    16141 ggtggttagc gtgctgcagg cgaaaaacta caccatcacc caacgtgatg atgctggtca
    16201 gacactgacc accgattggg tacaatggaa ccgtctggac gaagacgagc agtatcgtgg
    16261 tcgttatcaa atctctgtta agccgcaggg ttatcagcag gcggttacgg ttaaactgtt
    16321 gaacctggaa caggcgggca aaccggttgc agacgcggct tccatgcagc gttacagcac
    16381 ggagatgatg aacgttattt ccgccggtct ggataaatct gccactgacg ccgcgaacgc
    16441 tgcgcaaaat cgtgcctcca ccactatgga cgtacaaagt gcagctgatg acaccggttt
    16501 accaatgctg gtcgtacgcg ggccgttcaa tgtggtttgg caacgtctgc cagcggcgct
    16561 ggaaaaagtg ggcatgaaag tgaccgacag cacccgttcg cagggcaaca tggccgtaac
    16621 ttataagccg ctgtctgaca gcgactggca ggaactgggc gcgagcgatc caggcctggc
    16681 atccggtgac tataaactgc aggttggcga tttagataac cgcagcagcc tacagttcat
    16741 cgatccgaaa ggtcatactc tgactcagag tcagaacgac gcgctggtag ctgtcttcca
    16801 ggctgcgttt agcaagtaaa aatacagggc tggaatcatc cggccctttt ttctgatatg
    16861 atacgcaaac gtgtgcgtcg gcaggaaaac gcgattttag cggtaattcg cacgaaattt
    16921 gtttgtcgga cgtagttcgg ataaggcgtt cacgccgcat ccgacaaaac atccggcaca
    16981 ccagacagca aaagatttta aaacgttaat tcacacccag gagtgataaa gatgcaaaag
    17041 caagctgagt tgtatcgtgg taaagcgaaa accgtataca gcacggaaaa cccggacctg
    17101 ttggtgctcg aattccgcaa tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt
    17161 gatcgcaaag gtatgttgaa caacaagttc aactacttca ttatgagcaa actggctgaa
    17221 gcgggtatcc cgactcaaat ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag
    17281 ctggatatgg tgccggttga gtgtgtcgtg cgtaaccgtg ctgcgggctc tctggtgaaa
    17341 cgtcttggaa tcgaagaagg tattgagctg aacccgccgc tgttcgatct gttcctgaaa
    17401 aacgatgcca tgcacgatcc gatggtcaac gaatcttact gcgaaacctt tggctgggtg
    17461 agcaaagaga acctggcgcg tatgaaagag ctgacctaca aagcgaacga cgtgctgaaa
    17521 aaactgttcg atgatgctgg tctgattctg gtcgacttca agctggagtt tggtctgtac
    17581 aaaggtgaag tggtactggg cgatgagttc tccccggacg gcagccgcct gtgggacaaa
    17641 gaaacactgg agaaaatgga caaagaccgt ttccgccaga gcctcggtgg cctgatcgaa
    17701 gcctatgaag ccgtcgcccg ccgcctgggt gtacagctgg actgattttt ctgttcatca
    17761 tcttgccgtg cagctggcac ggcaagacaa ccgctctcgg aatatatgca ttgaacgctc
    17821 attcctccgc catttcatcc cgctttcctg tggtaatctt cctctgaacc acctacgatc
    17881 atttctataa tgaatatatt gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg
    17941 acaatgtcga agacaggcgt aacagctccg gcggtccgtc aatgggcggt cccggttttc
    18001 gcctgccaag cggtaaaggc gggctgattt tactgatagt cgtgctggtt gcaggctact
    18061 atggtgttga tttaaccggg ttgatgaccg gacagccggt ttcccaacag caatcaacac
    18121 ggtcaattag cccaaatgaa gacgaagccg caaaattcac ctcggtgatt ctggcaacca
    18181 cggaagacac ctggggacag caattcgaga agatgggtaa aacctatcag caaccgaagc
    18241 tggtcatgta tcgtggaatg acgcgtaccg gctgcggggc gggccagtcc attatgggac
    18301 cgttctattg cccggcggat ggcacggttt atatcgatct ctccttctat gatgacatga
    18361 aagacaaact cggcgcggat ggcgattttg cccaggggta cgttatcgcc catgaagtcg
    18421 gtcatcatgt gcagaaattg ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg
    18481 cgacgcaggc ggaagtaaac cgcttatctg tgcgtatgga actccaggcc gactgttttg
    18541 ccggtgtctg ggggcataac atgcagcagc aaggcgatct ggaagaggcg ctgaacgcgg
    18601 cgcaggccat cggcgatgac cgtttacaac agcaaagtca ggggcgagta gtaccagaca
    18661 gtttcactca tggtacttct cagcaacgct acagctggtt taaacgtggt ttcgacagcg
    18721 gcgatccggc acaatgcaat acttttggta aaagcattta acttccgggg caaggatggc
    18781 tgaactgact gcgcttcaca cattaacagc gcaaatgaaa cgtgaaggga tccgccgctt
    18841 gctggtgttg agcggggaag agggttggtg ttttgatcat gcgcttaagt tgcgtgatgc
    18901 cttacctggc gactggctgt ggatttcgcc gcagccagat gctgaaaacc actgttctcc
    18961 ctcggcgcta caaactttac ttgggcgcga gttccggcat gcggtattcg acgcccgcca
    19021 cggctttgat gccgctgcct ttgcggcact tagcggaacg ttgaaagcgg gaagctggct
    19081 ggttttgtta ctccctgtat gggaagagtg ggaaaaccaa cctgatgccg actcgctgcg
    19141 ctggagtgat tgccctgacc ctattgcgac gccgcatttt gtccagcatc tcaaacgcgt
    19201 acttacggcg gataacgacg ctattctctg gcggcaaaac cagccgttct cgttggcgca
    19261 ttttaccccc cgtactgact ggcaccccgc gaccggcgca ccacaaccag aacaacagca
    19321 actcttacag cagctactga ccatgccgcc gggcgtggca gcggtaacgg ctgcgcgtgg
    19381 gcgcggtaag tccgcgctgg cagggcaact catttctcgt attgcgggca gtgcgattgt
    19441 caccgcgccc gcaaaagcgg caacggatgt actggcacaa tttgcgggcg agaagtttcg
    19501 ctttattgcg ccggatgcct tgttagccag cgatgagcaa gccgactggc tggtggtcga
    19561 tgaagccgca gccatacctg cgccgttgtt gcatcaactg gtatcgcgtt ttcctcgaac
    19621 gttgttaacc actacggtgc agggctacga aggtaccgga cgtggttttt tgctgaaatt
    19681 ttgcgctcgc tttccgcatt tacaccgttt tgaactgcaa cagccgatcc gctgggcaca
    19741 gggatgcccg ctggaaaaaa tggttagtga ggcactggtt tttaacgatg aaaacttcac
    19801 ccatacacca caaggcaata tcgtcatttc cgcatttgaa caaacgttat ggcgaagcga
    19861 tccagaaacg ccgttaaagg tttatcagtt attgtctggt gcgcactacc ggacctcgcc
    19921 gctggattta cgccggatga tggatgcacc agggcaacat tttttacagg cggctggcga
    19981 aaacgagatt gccggggcgt tgtggctggt ggatgagggc ggattatctc aagaactcag
    20041 tcaggcggta tgggcaggtt ttcgtcgccc gcggggtaat ctggtggccc agtcgctggc
    20101 ggcgcacggc agcaatccac tggcggcgac attgcgcgga cggcgggtca gccggattgc
    20161 agtccatccg gcgcgtcagc gcgaaggcgt tgggcaacag ctcattgcca gcgctttgca
    20221 atataggcct ggcctcgact atctttcggt gagttttggt tacaccgggg agttatggcg
    20281 tttctggcaa cgctgcggtt ttgtgctggt gcgaatgggt aatcatcgtg aagccagcag
    20341 cggttgctat acggcgatgg cactgttacc gatgagtgat gcgggtaaac agctggctga
    20401 acgtgagcat taccgtttac gtcgcgatgc gcaagctctc gcgcagtgga atggcgaaac
    20461 gcttcctgtt gatccactaa acaatgccgt cctttctgac gacgactggc ttgaactggc
    20521 cggttttgct ttcgctcatc gtccgctatt aacgtcgtta ggttgcttat tgcgtctgct
    20581 acaaaccagt gaactggcat taccggcgct gcgtgggcgt ttacagaaaa acaccagtga
    20641 cgcgcagtta tgtaccacac ttaaactttc aggccgcaag atgttactgg ttcgccagcg
    20701 ggaagaggcc gcgcaggcgc tgttcgcact taatgaggtt cgcactgagc gtctgcgcga
    20761 tcgcataacg caatggcaat tttttcactg actccttcaa ttttctccca tggtcattgt
    20821 gagtcagcgg cgtaaaatta atgacatcag ttaaggagtt cgccatgaaa catgaccatt
    20881 ttgttgttca aagcccggat aaacctgcgc aacagttgtt actgcttttc cacggtgtcg
    20941 gggataaccc ggtggcaatg ggggaaatag gcagctggtt tgcgccgcta tttcctgatg
    21001 cgctggtggt gagtgtcgga ggcgcggagc cgagcggtaa tccggcgggg cgtcagtggt
    21061 tttcggtgca gggtattacg gaagataatc gccaggcgcg tgtggatgcc atcatgccga
    21121 cgtttattga gatggtgcgc tactggcaga aacagagcgg agtaggggct aacgccacag
    21181 cgctaatcgg tttctctcaa ggcgcgatta tggcgctgga gagcattaaa gccgaaccgg
    21241 gccttgcttc acgcgtcatt gcttttaacg gacgctatgc gagcctgccg gaaactgcgt
    21301 ctacagccac gacaattcac ctgattcatg gcggtgaaga tccggttatc gatctggcgc
    21361 atgcggttgc ggcgcaggaa gcgttaatca gtgccggtgg tgacgtgacg ctggatatcg
    21421 tggaggattt aggccatgcg atcgacaatc gcagtatgca atttgccctg gatcacttgc
    21481 gttataccat tccgaaacat tatttcgacg aagcgctaag tggcggtaag cctggcgatg
    21541 atgatgtgat tgaaatgatg taaaagacag attacctggc ctgataagac gcgtgagcgt
    21601 cgcatcaggc atcgttcacc attgccggat gcggcgttaa cgccttatcc ggcctacggt
    21661 tgagcactca gcttacttct tcggttgatc ctttttcggc cagtcgtcgt catcgtccca
    21721 cttatcgttg aaatcacgat gcgggggaag ctccggcttg ttggcgagaa atttcttgtg
    21781 gtcgacgcgc ttgagatctt taatcacgtt cagcaggacg cctaccaaaa agacaatcac
    21841 cagaatccac caatatttag ccagccagtc catgcttatt tcctcttgca gaaccactca
    21901 tcaggcgacg agctgttcca tgatacgttg atacatacgg gcaagtagct gcaggtcggc
    21961 agcgttcaca cattcattaa ttttatgaat agtggcattg accggcccga gttccaccac
    22021 ctgcgccccc atgcgagcaa taaagcgccc gtcggacgtt ccgcctgtgg tcagtagctg
    22081 cggtttaatt tcattatagt gctcaaccgc gttaacgacc gcatccacca gtttaccgcg
    22141 cgcggtcaaa aatggctgcc cggaaagcca ccaatccacc gtatagcgca gttgatgttt
    22201 ttcaagcagg gcaagcacct gcgctttgat catctcatca gtcagttcgg tgctgaagcg
    22261 gaagttaaac tgcacaaaca gttcacccgg aataacgttg ttactgcccg ttcccgcctg
    22321 aatattggca atctgcatac tggtcgccgg gaagaattca ttgccctgat cccactcaat
    22381 agccactaat tcattaagga aaggtgctgc gcgatgtact ggattgtcag ccagatgtgg
    22441 gtaggccaca tgcccctgca cgccatgaat ggtaaggttg caggttaacg atccgcgacg
    22501 accatttttc accacatcac caaccacttc gatactcgac ggttcgccga ccagacagta
    22561 atcgagacgc tcattacgcg ccattaacgc ttcgacgact tttaccgtac cgttgtgggc
    22621 actggcttct tcatcagagg tgatcagaaa tgccagtcgt cccgtatggt tgggatgttg
    22681 tgcgacaaaa cgttctgctg ctaccaccat cgccgccagc gagcctttca tatctgccgc
    22741 accgcgcccg aataacatgc catcacgaat ggtgggttca aacggcggat tgatccaacg
    22801 atcggcgtcg ccaggcggca ccacgtcggt atgcccggca aaggccaatg tttcaccttg
    22861 cccacgccat gcccaaaaat tctgcgtatc ggcaaagtcc atgcgttcaa cggtaaaacc
    22921 gatcgcctgc aaacgatcaa tcaacaaagc ctggcatcct gcgtcatcag gactcaggga
    22981 agggcggcga ataagctgtt gtgtcagctc aataaccggg cacgacatag actacacctc
    23041 atggaaaaat tgctgataac tggaatcact gaaacccagc agcataggct taccgggcgc
    23101 gcagagcaat ggacgtttga taattgcagg catttcagtc attaatgccg ccgctgaggc
    23161 cgcatcggtg attttattgc gggtggtttc gtccagttta cgccaggttg taccacgggt
    23221 gttgagtaac gcctcccaac ctaattcgtt gataaaaccg ttcaataatt cgctgtccag
    23281 cccatcgacg cggtaatcat gaaaacgata gtcgatgtta ttggcttcca gccaacggcg
    23341 agccttttta atggtgtcac aatttttgat accgtaaagt gtaaccataa tgaatccttt
    23401 ttcgatttat gagaataaat actcatttaa gggtgaaaac attttgcctg caaatgatta
    23461 tcaggaaata taacattatc gaagtattca aattaacaac tattaaataa tattaaacat
    23521 ttttaacgta aacattaaga atattcatct tgttatctgt ctgtatttta acctgaatta
    23581 aatcagtatt agatgccgta tgaatatctc gaaatgttgc tgtgtatcac atacaaatca
    23641 tcaccgcagg accatagtat ttacataaga tttgtccccc cggaggtagt catcgcagca
    23701 atcgagtaaa ttagcttcac cacggtatat tgtttccgta gaatacccat aattataaga
    23761 gaggttgtta tgattgaacg tgaactgggg aactggaaag actttatcga agttatgctt
    23821 cgtaagtaat tcctgagaca aagtataaac agcaagaacc cgctgagcag gttcttaatc
    23881 ggaaagttga aaaaggcgac acattggcat gtcgcctttt ttattgctta ttccgggcgc
    23941 ggctttagcg ggaagcggcg gcgcaccagc acaaagaaca gcggcacgaa gtaaatagcc
    24001 agaatagtgg ctgaaatcat cccgcccatt acgccagtac ccaccgcatg ctgaccaccg
    24061 gaaccggcac ccgtgctggt tgccattggc aatacgccga agataaatgc cagcgaggtc
    24121 atcagaatcg ggcgtaaacg ctgacggcag gcgtggagcg tcgcttcaaa caggtcgtgg
    24181 cctttttggt tcatctcgtt agcaaactcg acgatcagga tggcgttttt cgccgataaa
    24241 ccaatgaccg ttaacaggcc cacctggaag taaacgtcgt tttccagccc gcgcatccag
    24301 gttgccagca gcgcgccgat tacccccagc ggcacgacca gcattaccga gaacggcacc
    24361 gaccagcttt catacaacgc agccagacac aggaatacca ccagcaagga aatggcgtac
    24421 agcgccggag cctgcgcgcc ggaaagtcgc tcctgatacg acatcgccgt ccactccaga
    24481 ccaaagccgt ttggcagctg cttcactaac gattccataa tatccatcgc cgtaccggta
    24541 ctgacccccg gtgcggcttc cccaacaatc tcaaccgcag aataaccgtt atagcgttcc
    24601 agacgcggcg agcctgtttc ccagcgtgag gtcgcgaaag cagagaaggg caccatgccg
    24661 ccatctttat ttcggacata ccagagattg atgtcatctg gcagcatgcg atacggcgca
    24721 gctgcctgca catagacttt cttcacgcga ccgcgatcca taaagtcatt cacatagctc
    24781 gaaccccagg cggtttgcag tgtgtcgtta atatcgtcga tagcaacgcc cagcgcctga
    24841 gctttacgct ggtcgatatc aatctgcaac tgcggactgt cgtcgaggcc gttatggcgc
    24901 acacgggtta gctccgggtt ttccgccgcc agcgccagca actggttacg tgctgccatc
    24961 agcgcatcgt gacctgctcc agcgtggtcc tgcaactcca tatcaaaacc cgcagaacta
    25021 ccaagaccgc taattgctgg cgggctgctg gcgataacgc gagcttcttt aatcttgtta
    25081 aacgcctttg ttgcacgctc gataatggca aacgaggtgc cggtcttact gtcgcgttcg
    25141 ctccagtctt tcaggcggat aaacattcgc gccacgtttt gcccgttacc cccagggcca
    25201 gaaccaacgg tggcaaacac cgacatgatg ttgtcttttt catgggtgaa gtaatatttc
    25261 tcgatttgct caacgacttt cagggtctgt tgttgggttg aaccgctggg caattgtacc
    25321 gaggtagtaa acatgccacg gtcttccagc ggtaagaacg acgtcggcaa acgcaggaac
    25381 aggaacacca tgccgccaag cagcaggaca taaatcacaa tccagcgcag gctgcggtgg
    25441 agaattttcg ccaccccttt ttcgtagcgt tcggcgttgc ggttaaacat ctggttaaac
    25501 caggcaaaaa agcctttttg cccgtgatgc tcaccttttt tcagtggctt aagtagtgtg
    25561 gcacacagag ccggagtgag gatcatcgct accagtactg acagcaccat cgccgcaaca
    25621 atggtaatag agaactggcg atagatggca ccggtggtgc cgccgaagaa ggccattggt
    25681 acaaataccg ccgaaagcac catcgcaatc ccgaccagtg ccccctggat ctgccccatc
    25741 gatttacgtg tggcttcgcg aggagtgagt ccttcctcac tcataatacg ttcgacgttt
    25801 tccaccacca cgattgcgtc atccaccagc agaccgatcg ccagtaccat cgcgaacatg
    25861 gttaaggtgt tgacgctgta accgaaggcg taaagtacgg agaaggttcc catcaacacc
    25921 accggcacgg cgatagtggg tatcagcgtg gcgcggaagt tttgcaggaa cagatacata
    25981 acgaggaaaa ccagggcgat agcttccagc agcgttttca ccacgtcttc aatggaggct
    26041 ttaacaaacg aggtggtttc atacgccacc ttgtattcca gtccgtgcgg gaagtactgc
    26101 gccagctcgt cgagacgatt gagcaccagc tccgctgtcg ccatttcgtt tgcgccggag
    26161 gccagtttta cccccagccc ggaggctggc ttaccattga agcggctaag ataatcgtat
    26221 ttctccgccc ccatttcgac ggtggcgaca tcgcccagcc ttacctctga gccgtcttga
    26281 ttcacccgca aggtgatatc gcggaactgt tctggtgttt gcagcagtga ctgggcgtta
    26341 atggtggcgt tgagcgcctg cttatcgacg gaaggtgtac caccaagttg cccaaccgca
    26401 atctgcgcgt tctgtgactc aatggcatca gtgacatctt tagccgtcat ctggaaactg
    26461 ttgagtttcg ccgggtccag ccagatacgc atggaatatt gcgaaccata ggcatcgata
    26521 tccccgacgc cattcacgcg gcttaacggg tcctgaatat tactggcaac ataatcggca
    26581 atatcctgtt tatccatcga accatcggta gagacgaaag caatggtcag aatattggta
    26641 tcgccggttt tacgcaccgt cacgccctga ttttgcaccg cctgcggtaa ctttcgcatg
    26701 gctgattgca gctggttttg tacttgctgc acggcttcgt ccggatcggt gcctgcttta
    26761 aaacttaaag tgacagatgc ctgaccgatg ccactgctct gagatgacat atacatgaga
    26821 ttatcgaggc cggtcatatt ttgctcgata acctgggtca cggtgttttc cagcgtctgg
    26881 gccgatgcgc cgggatagtt agcggtcact cgcacattcg gtggcgcgag atcggggtat
    26941 tgttcaacgg gcaatgaaaa aatcgccagg gtccctgtca gacacaacag gattgccagc
    27001 acccaggcaa aaatggggcg atcaataaag aaattcgcca ttaaaagagg acctcgtgtt
    27061 tctgcgtatc gttgtaggct tgacttgctt cactgtagcg gcaaggtacg agccaatcgt
    27121 ggagaaaaaa aggagttaat gtaaattatc atgccctttt atactgaacc ttaagtgcaa
    27181 gtattctttg gtcagtagga ggcattacat caactgactt tcctcaccct ccgcagagcg
    27241 aaagctaata ctcactaacg tgccgccgcc ggaaggttgt gaaaaagtca gcgtcccacc
    27301 cagccgttcc gcgcgttcgc gcatgatatt cagaccataa tgaccttcgg gttctttcgg
    27361 ttcaccgata ccaatcccgt tatcacggat ataaaccgtg tgattgccgt ccggcgcggt
    27421 gacgcaactg acggcgattt cgctggcgtt ggcgtgcttc atcgcattca gcaccgcttc
    27481 gcgaataatt tgcaacaaat gcacctgcat ttgcgcatcc agcgccaggg ttggcagacg
    27541 gcagtcgagg gtcagtttgg cgctggtttg attttgtaac gtatccagca tttccctcaa
    27601 cgcggagggg agatccgcct gctgcagcgt caggcggaaa gtagtcaaca gctcgcgtaa
    27661 ctgccgataa gcatcattca acgcctggga aaaatcggcc atgatacttt gtgcggtggc
    27721 gttatcttcc ggtatcgaac gcttcagtaa cgtcaactgg atacgtaagt aagaaagtac
    27781 ctgagccagc gagtcgtgca attcgcgggc gatggttgca cgttcttcca tcaacaataa
    27841 ctgctgaaaa tgcttttgcg cctgattaaa gtacaaaccg cgtcccagca tcgacgaaac
    27901 gctgttaagc agcggttctg atgatgaaac gtgactattt tgccagtgca gttcgccgta
    27961 aaccgtctct tgcattgtca ccggtaaaat ctgcatcggc aattccgggt ttggttgccc
    28021 ttcgctaatc cgccagtttt caccgacatt taactccaga tattcagccg cttcattgtc
    28081 gcgaacaatc tgcaaaatat ggcggaaaca atgcacatca atctggcttg tgttcagcgc
    28141 ctgcgaacat tgatacaaca cctccagacg acgcttggcc tcgtggagat cgcgggtctt
    28201 ttcttctact gacgcctcca gcgaaaggta caatttatgc agctcgctcg acatctggtt
    28261 aaaggttttt gcaagcagac caagctcatt cggcagactg gtatccagcg gcggcgagtc
    28321 gaactgcccg tgttcaatac gttgactggc ggtaaccagc tgattcagcg gggcaaccac
    28381 ctgatggcgt atacggcgca aagtaaaaaa gaccagcgtg aaaataccga taccgccagc
    28441 cagggaaatc gccaccacca gcagcatttt gcgttcagcg tagtgctgta aagccagcac
    28501 gaacaggtct atctgattaa cataattatt aatattggcc tgataccacg gcaaatcgcc
    28561 cttgctgagc cgattattca tctccagcca gttggcattc agatgcgcat agcgagtttt
    28621 tactgcttct ggcacatacc agacgttgag attggttaat accgatgaat gcagtgcctg
    28681 ctgaaacaac tggcgatgtg cattgagttg tggactgcca ctttgcaagt cgtagcccag
    28741 gcggtaactc tgcatacgca gcgatccggc aatattgata gcctcagcgt cgcgcaaact
    28801 gctcgccaga gttagcagag cgatacccgt ggaaagaatc gacagcagca caatgtaaaa
    28861 aaaggcccgg gccagactgg ccgagacggg tcgtttaaca atcacacgcg tcttctccac
    28921 aaaaaatcat taatgttcca gttcgctaaa acgcaattta gtcagtgatg ggattatttg
    28981 atctgccaca gtttatagcg aaatagttac ttattctggg cacatgaaca ttgccgattc
    29041 ccctgtaagt cgggtaataa caacactcat ataaagaata aggtttttac aaccaaaaaa
    29101 gaaggtcgtt atgaatcgtt ttattatggc caacagtcag caatgtctgg gttgtcatgc
    29161 ttgtgaaatc gcctgtgtca tggctcacaa tgatgagcaa catgtcctga gccaacacca
    29221 ttttcatccc cgaattacgg ttatcaaaca tcagcagcaa cgtagtgcag tgacctgtca
    29281 ccattgtgaa gatgcgccct gcgcccgtag ctgccctaat ggcgcaatca gccacgttga
    29341 tgacagcatt caggtcaatc agcaaaagtg tattggctgt aaatcctgcg tggtggcctg
    29401 tccgtttggt acgatgcaaa tcgtcctgac acccgtcgcg gcaggaaaag taaaagccac
    29461 ggcgcataaa tgcgaccttt gtgcagggcg cgaaaacggt cctgcctgtg tcgagaattg
    29521 cccggcggac gcgctgcaac tggtcactga cgtcgcactc tccggcatgg cgaaatcccg
    29581 ccgcttgcgc accgcacgtc aggagcatca accgtggcat gccagtaccg ctgcacaaga
    29641 aatgccggta atgagtaaag tcgaacaaat gcaggcaacg cccgcgcgtg gcgagccgga
    29701 taaactggcg attgaagcgc gcaaaaccgg ttttgatgaa atttatctgc cattccgtgc
    29761 cgatcaggca caacgggaag cctcgcgctg ccttaaatgc ggcgagcaca gcgtttgtga
    29821 atggacctgc ccgctgcata accatattcc gcagtggatt gaactggtga aagccggaaa
    29881 catcgacgcc gccgtcgagc tttctcacct gaccaacacc ctgccggaaa ttaccggacg
    29941 cgtttgtccg caggaccgtt tgtgtgaagg tgcctgtact attcgcgatg agcacggcgc
    30001 ggtaactatc ggcaacattg aacgctacat ttcagatcag gcgttggcga aaggttggcg
    30061 tcctgactta agccatgtca ccaaagtgga caagcgggtg gcgattatcg gtgcgggtcc
    30121 ggcagggctg gcctgtgcgg atgttctgac ccgcaatggc gtggcggtga cggtgtacga
    30181 tcgccatcca gaaattggtg gcttgctcac tttcggcatt ccttctttca aactggataa
    30241 atccctgctg gcacgccgtc gggaaatctt cagcgcgatg gggattcact tcgaactcaa
    30301 ttgtgaagtg ggtaaagatg tctctttgga ttcgcttttg gaacaatacg acgcggtctt
    30361 cgttggcgta ggcacttacc gttccatgaa agcgggttta cccaatgaag atgcgccggg
    30421 cgtttatgac gcgctgccgt tcctcatcgc caacactaaa caggtgatgg gactcgaaga
    30481 gctaccggaa gagccgttta tcaataccgc cggactcaac gtcgtggtac tgggcggcgg
    30541 cgacaccgcg atggactgtg tgcgtaccgc gctgcgccac ggcgcgagta acgtcacctg
    30601 cgcttatcgt cgtgatgaag ctaacatgcc aggctcgaag aaagaggtga agaacgcccg
    30661 cgaagagggg gccaacttcg aatttaacgt ccagccggtg gcgcttgagc tgaatgagca
    30721 aggtcacgtt tgcggaattc gtttcctgcg cacgcgtctt ggcgagccgg atgcccaggg
    30781 gcgccggcga cttgtaccga ttgagggcag tgaatttgtc atgccagccg atgcggtaat
    30841 tatggcgttt ggcttcaatc cacacgggat gccgtggctg gagtcgcacg gtgtaacggt
    30901 agacaaatgg ggccgcatca tcgcggatgt ggaaagccag taccgttacc agaccaccaa
    30961 tccgaaaatc ttcgccggtg gcgacgccgt gcgtggtgcg gatctggtgg ttaccgcaat
    31021 ggcagaagga cgtcatgcgg cacaggggat tattgactgg ctgggggtaa aatcagtcaa
    31081 atctcactga tagcctgcgc agacaaaccc gacttcacag cgtaatataa ttgttcattt
    31141 cgcgctgtgg agtcggtatg acgcaacaaa tcaccctcat taaagacaaa attctctccg
    31201 ataactattt caccctgcac aacattactt acgatctcac gcgcaaagat ggctaagtta
    31261 tccgccataa acgtgaagtt tacgatcgcg gtaatggtgc gacgatcctc ctgtacaacg
    31321 cgaagaaaaa gaccgtggtt ctgattcgtc agttccgtgt cgctacctgg gttaatggca
    31381 atgaaagcgg gcagctgatt gaaacctgcg ccgggctgct ggataacgac gaaccggaag
    31441 tgtgcattcg taaagaagcg attgaagaga cgggctatga agtgggcgaa gtgcgcaaat
    31501 tatttgaact gtatatgtcg cctggcggtg tgactgagct aatccacttt tttatcgccg
    31561 aatacagtga caatcagcgc gctaacgccg gtggcggtgt tgaagatgaa gatattgaag
    31621 tgctcgagct gccgttcagc caggcgctgg agatgatcaa aaccggcgag atacgtgacg
    31681 gtaagacggt gttattgctt aactatttgc aaacgtcaca tttaatggac tgaaaaataa
    31741 caataatatt tcgttgttta ttattggctc aatccgacaa atctgattga gccgcgctac
    31801 ctgcgcaacg aagatacgac ttgtgctgtt tgtttgaact tctggggtcg taccgtccat
    31861 gcgctatcgc attttccttc tctttttttt cgctttgttg ccgacgtctt tggtgtgggc
    31921 ggcactagcg caacgggcgt tttccgactg gcaggtcacc tgtaataacc aaaacttctg
    31981 cgtggcgcgc aatacgggcg atcataatgg actggtgatg accctgagcc gcagcgccgg
    32041 ggcgcatacc gatgccgttt tacgtattga gcgcggcggg ttgaagtcgc cggatgcgtc
    32101 tgaaggagag atagcgccac ggctgctgtt agatggtgag ccgttggcgt taagtggtga
    32161 caagtggcgg atttcaccat ggttattagt aaccgatgat acggcaacca tcaccgcgtt
    32221 tttgcagatg attcaggaag ggaaggcgat caccttacgc gacggcaatc agaccatttc
    32281 tctgagtggc ttaaaagcgg cgttgttgtt tattgatgct cagcaaaagc gcgttggcag
    32341 tgaaaccgcg tggatcaaga aaggggacga accgccgctc agcgtaccgc ccgcgcctgc
    32401 gctgaaagag gtcgcggtgg ttaacccaac gccgacgcca ctctcactcg aagaacgcaa
    32461 cgatttgctg gattatggaa actggcggat gaatggtctg cgctgctcgc ttgatccgtt
    32521 acgtcgtgag gtgaatgtca ctgcgctgac tgatgataaa gcgctgatga tgatcagctg
    32581 tgaggcaggg gcctataaca ccattgattt ggcatggatt gtgtcgcgta aaaagccgct
    32641 agcctcgcgc ccggttcggt tgcgtttgcc gttcaacaac gggcaggaga cgaatgaact
    32701 ggaactgatg aacgcaacat ttgatgagaa atcgcgtgaa ctggtgacct tagcgaaagg
    32761 gcgcggatta agcgattgtg gcattcaggc gcgctggcgc tttgacggtc agcgttttcg
    32821 cctggtgcgt tacgccgcag aacccacctg cgataactgg catgggccag acgcctggcc
    32881 cacgttgtgg atcacccgtt aaacaaaatg gcggggacat gtaggccgga taagacgctg
    32941 taggccggat aagacgcgtc agcgtcgcat ccggcaatca gcatccggcg atcactatca
    33001 ggcacctttc actcccagca ccttatgcgc ttttgccaca atattctcgg cggtaaagcc
    33061 aaagaacggg aacagcttat ccgccggagc agattcccca taacccgtca tcccgacaat
    33121 tgcccctttc agaccaacat acttgtacca gtaatcggca atacctgctt ccaccgccac
    33181 gcgagccgca acgttagaag gcaacaccga ctcccgatat tcctcatcct gggcgtcgaa
    33241 aatatcggtc gagggcaggg aaaccacgcg aacattgcga ccttctcctg ctaatttctc
    33301 cgcagcttgc agggtgattt ccatctctga accggtggca atcagaataa tatctggctt
    33361 accgccgctg tctttcagca cataaccgcc acgagtaatc tctttaacct gatccggcgt
    33421 acgttccacc tgggccagat tctgccttga gaggatcagt gccgtcggtc cgttgtggcg
    33481 ctcaaccgcc agcttccagc ccaccgccgc ttccacctga tcgcacggtc gccaggtgct
    33541 gaaatttggc gttaagcgca ggctggccag ttgctcaaca gcctggtgcg tcggaccatc
    33601 ttcgcccagg ccaattgagt cgtgggtata aaccataatc tgccgcgctt tcatcagtgc
    33661 cgccatccgc gcggcgttac gggcgtattc aacaaacatc aggaacgtcg cggtatacgg
    33721 cacaaagccg ccgtggtgcg cgatgccgtt ggcgatagcg gtcatgccaa attcacgcac
    33781 cccgtagtga atgtagttgc ccgccggatc ttccttcagc gaaacagaac ctttccagat
    33841 ggtcaggttg ctgggagcca gatccgccga accgccgagc agctccggta gcaaggggcc
    33901 ataagcgtta agcgtatttt gcgaagcctt acgggtggcg attttcgccg gattcgcctg
    33961 caactcattg atatacttct gagtcgtttt ctcccagtcc ttcggtaaac caccgctcat
    34021 ccgacgggta aactcttctg ccagttgcgg atgagccttt ttataggcgg caaacttctc
    34081 attccacctc tgctgcgctt tttcgccttt ttcgcgggca tcccaggcgt gataaatatc
    34141 tttagggatc tcaaatggcg gatggtgcca gcccagtttt tgccgtgcca gcgccacttc
    34201 ttcttccccc agcggtgcgc cgtgcgcctc ttccttacct gctttattcg gcgatccaaa
    34261 gccaatcacc gtacgacaga taatcagcga cggcttatct ttcacgcttt gcgcttcaag
    34321 gatcgcttcc ttcaccgcct gcggatcgtg accgtcgatt tcatggatca catgccagtg
    34381 ataggcttca aaacgttttg ccgtatcgtc ggtaaaccag ccttctgttt caccgtcgat
    34441 ggaaataccg ttgtgatcgt aaaaaccaat cagcttgccc agtcccagcg tgcctgccag
    34501 cgaacagact tcgtgggaaa taccttccat caggcagccg tcgcccataa acacataggt
    34561 gaagtgatcg acgatctcat ggtctggctg gttaaactgc gccgccagtg tacgctctgc
    34621 tatcgccagc ccgacggcgt tcgccaaacc ttgtccaagt ggaccggtgg tggtttcaac
    34681 gcctggcgta taaccaatct ccgggtggcc tggggttttc gaatgcagtt gacgaaagtt
    34741 cttcagctct tccagcggca ggtcgtaacc ggtcagatgc agcaaactgt agagcagcat
    34801 cgacgcgtga ccgttggaaa gaataaagcg gtcgcgatca taccaggttg ggtcggtagg
    34861 gttatgttta agaaaatcgt tccacagcac ttcggcaata tcagccatgc ccatcggcgc
    34921 gccgggatga ccagaattgg ctttttgtac cgcatccata ctgagtgcgc gaatcgcatt
    34981 ggcaaggtct tttcgggaca tataacactc cgtggctggt ttatagtttg gcggcaagaa
    35041 gatcttccag tttgcgttga tcaacggcga acagacgaat gccttcagac agtttttcta
    35101 ccgccatcgc atactgattg tgctcccaac ggaactccgc ttcgctcatg ggagctgggc
    35161 gtgggaacgt ctgagaaggt gggattaatt tacgtaccac tggcgaaact ttttcctgca
    35221 gctccttcag caaattcggt gcgatagtca gtcgatcgca gccggttaag gcgaggattt
    35281 gttcggtgcg acggaagctc gcgcccatca caatggtttc atagtggtgt tgcttatagt
    35341 agtcgtagat attgcgcacc gatttaacgc ccggatcttc ttccaccaca tacgggtcca
    35401 tcggcttgcg tgcctgatac cagtcataaa tacgcccgac aaacggcgaa atcagaaaaa
    35461 cgcctgcttc cgcacaggcc cgtgcctgtg caaaagaaaa cagcagcgtc aggttgcagt
    35521 taataccttc tttttccagc tcttctgccg cgcgaattcc ttcccaggtc gaagccagct
    35581 tgatcagaat gcgtgatttc tcaacgcctt gttgctgata caagtccacc agatggcgcg
    35641 ccttctcaat actcttttct ttatcaaaag agaggcgtgc atcaacttct gttgacacgc
    35701 gaccgggtac gattttgagg atttcagcac cgaaattgac cgccagtttg tcacacgctg
    35761 cgaccacctg ttgttcctgg gtcttgccat tttttttacc ccaggcgata gcatcgtcta
    35821 ttaaatgctc atattgtgat aatccggcag ccttgagtaa cagcgaagga ttggtggtgg
    35881 catcctgggg atgataatgg cgaatggact caatatcgcc gctgtctgcc acgacagtgg
    35941 tgaactgttt gatgccgtct aactcgttca taggaaatac tccttgaaaa gtaaagtgtt
    36001 agatgagtgc gttaattcac acttctgaga aatttcgcta aacgcatcaa aaaagcatag
    36061 cagacaggca tggtattgct ggattaagca ggtaacatca gtgttatagg attattacca
    36121 aaacattata ttaatggtga gagtttggac ttgctcaaag tctgtagact ccggcaaggt
    36181 aataatgtgc gccacgttgt gggcaggggg tgttgcccac acactttatt tgtgaacgtt
    36241 acgtgaaagg aacaaccaaa tggatgacca gttaaaacaa agtgcacttg atttccatga
    36301 atttccagtt ccagggaaaa tccaggtttc tccaaccaag cctctggcaa cgcagcgcga
    36361 tctggcgctg gcctactcac ccggcgttgc cgcaccttgt cttgaaatcg aaaaagaccc
    36421 gttaaaagcc tacaaatata ccgcccgcgg taacctggtg gcggtgatct ctaacggtac
    36481 ggcggtgctg gggttaggca acattggcgc gctggctggc aaaccggtga tggaaggcaa
    36541 gggcgttctg tttaagaaat tcgccgggat tgatgtattt gacattgaag ttgatgaact
    36601 cgacccggac aaatttattg aagttgtcgc tgcgctcgaa ccgaccttcg gcggaatcaa
    36661 cctcgaagac attaaagcgc cagaatgttt ctatattgaa cagaaactgc gcgagcggat
    36721 gaatattccg gtattccacg acgatcagca cggcacggca atcatcagca ctgccgccat
    36781 cctcaacggc ttgcgcgtgg tggagaaaaa catctccgac gtgcggatgg tggtttccgg
    36841 cgcgggtgcc gcagcaatcg cctgtatgaa tctgctggta gcgctgggtc tgcaaaaaca
    36901 taacatcgtg gtttgcgatt caaaaggcgt tatctatcaa ggccgtgagc caaacatggc
    36961 ggaaaccaaa gccgcatatg cggtggtgga tgacggcaaa cgtaccctcg atgatgtgat
    37021 tgaaggtgcg gatattttcc tgggctgttc cggcccgaaa gtgctgaccc aggaaatggt
    37081 gaagaaaatg gctcgggcac caatgattct ggcgctggcg aatccggaac cggaaattct
    37141 gccgccgctg gcgaaagaag tgcgtccgga tgccatcatc tgcaccggtc gttctgacta
    37201 tccaaaccag gtcaacaacg tcctttgctt cccgttcatc ttccgtggcg cgctggacgt
    37261 tggcgcaacc gccatcaacg aagagatgaa actggcggcg gtacgcgcga ttgcagaact
    37321 ggcccatgcg gaacagagcg aagtggtggc ttcagcgtat ggcgatcagg atctgagctt
    37381 tggtccggaa tacatcattc caaaaccgtt tgatccgcgc ttgatcgtta agatcgctcc
    37441 tgcggtcgcg aaagcggcga tggagtcggg cgtggcgacg cgtccgattg ctgatttcga
    37501 cgtctacatc gacaagctga ctgagttcgt ttacaaaacc aacctgttta tgaagccgat
    37561 tttctcccag gctcgcaaag cgccgaagcg cgtagttctg ccggaagggg aagaggcgcg
    37621 cgttctgcat gccactcagg aactggtaac gctgggactg gcgaaaccga tccttatcgg
    37681 tcgtccgaac gtgatcgaaa tgcgcattca gaaactgggc ttgcagatca aagcgggcgt
    37741 tgattttgag atcgtcaata acgaatccga tccgcgcttt aaagagtact ggaccgaata
    37801 cttccagatc atgaagcgtc gcggcgtcac tcaggaacag gcgcagcggg cgctgatcag
    37861 taacccgaca gtgatcggcg cgatcatggt tcagcgtggc gaagccgatg caatgatttg
    37921 cggtacggtg ggtgattatc atgaacattt tagcgtggtg aaaaatgtct ttggttatcg
    37981 cgatggcgtt cacaccgcag gtgcaatgaa cgcgctgctg ctgccgagtg gtaacacctt
    38041 tattgccgat acctacgtca atgacgaacc agacgcagaa gagctggcgg agatcacctt
    38101 gatggcggca gaaactgtcc gtcgtttcgg tattgaaccg cgcgttgctc tgttgtcgca
    38161 ctccaacttt ggttcttctg actgcccgtc gtcgagcaaa atgcgtcagg cgctggaact
    38221 ggtcagggaa cgtgcgccag aactgatgat tgatggtgaa atgcacggcg atgcggcgct
    38281 ggtggaagcg attcgcaacg accgtatgcc ggacagccct ttgaaaggtt ctgccaatat
    38341 tctggtgatg ccgaacatgg aagctgcccg cattagttac aacttactgc gcgtttccag
    38401 ctcggaaggt gtgactgtcg gtccggtgct gatgggcgtg gcgaaaccgg ttcacgtgtt
    38461 aacgccgatc gcatcggtgc gtcgtatcgt caacatggtg gcgctggccg tggttgaagc
    38521 gcaaacccaa ccgctgtaat tcttttttaa ctctcacgct taccctgaat attcagggta
    38581 agcggtttgg ctgcaatata ttagtaaagc ttattactga gtttgcgaat aataaaaaaa
    38641 agcagtctat ataatatctc gatattattt tatttatatt catgcgttgc atatgaaagt
    38701 ttatgcacca cagcgaatat ctctccattc ttagtgatct acctcacctt ttaaacgcgc
    38761 ttgccgaatt ttgttattta ctctgacgaa aaattgtcac gatacacgaa agtttttcac
    38821 aggcggcgac tcatggataa agaacgcatc attcaggaat ttgtgcccgg caaacaggtc
    38881 acactggcgc atctcatcgc gcatcccggt gaggaactgg cgaagaaaat cggcgttccg
    38941 gatgcgggcg caattggcat tatgacgcta actcccggcg aaacggcgat gatcgtcggt
    39001 gatttagcgc tgaaagccgc tgatgtgcat atcggttttc tcgaccgctt cagcggtgca
    39061 ttggtgattt atggctcggt gggcgcagta gaagaagcgt tatcacaaac cgtcagcggt
    39121 ttaggtcgct tattaaatta tacgctttgt gaaatgacca aaagttaata cgaggcattc
    39181 atgaaacgaa ttgcgtttgt gggtagcgtc ggggcaggaa aaacaacgtt atttaatgcg
    39241 ttgcagggga attataccct cgccagaaaa acacaggccg tggaatttaa tgataagggc
    39301 gatattgata cgccgggtga atattttagc catccccgct ggtatcacgc cttaattacc
    39361 acgctacagg atgtagacat gctgatctat gtccatggcg caaatgaccc ggaaagtcgg
    39421 ttgcctgccg ggttgctgga tattggcgtc agcaaacggc aaatcgccgt catcagtaaa
    39481 acggacatgc cagatgctga cgtcgccgca acgcgaaagt tattgcttga gacgggtttc
    39541 gaagagccga ttttcgagct aaacagccac gacccgcaaa gcgtgcagca actggtggat
    39601 tatctggcat ctctcaccaa acaggaggaa gcaggtgaaa aaactcatca cagcgaatga
    39661 tattcgtgaa gcacacgcgc gcggcgaaca ggcaatgtcc gtggttctgc gcgccagcat
    39721 tattaccccg gaagcccgta aagtggcgga tctactgggt tttaccatca ccgaatgtga
    39781 tgaatcgatc ccggtaacgg cgtccgtgcc cgccagcgtg ccagccgaca aaaccgaaag
    39841 ccagcgcatc cgcgaaacca tcattgccca gctgccggaa ggccagttta ccgaaagcct
    39901 ggtcgcacag ctgatggaaa aagtgatgaa ggaaaaacag tcgctggagc agggcgcaat
    39961 gcagccgagc ttcaaatcgg tgaccggcaa aggcggcatc aaagtgattg atggcagcag
    40021 cgtcaagttt ggtcgttttg atggcgcaca accgcattgc gtgggcttga ctgatcttgt
    40081 gaccggagac gacggcagca gcatggccgc cggttttatg cagtgggaga acgcattctt
    40141 cccgtggacc ctgaactacg acgaaatcga catggtgctg gaaggggagc tccatgttcg
    40201 ccacgaaggc gaaaccatga tcgccaaagc aggcgacgtg atgtttatcc cgaaaggctc
    40261 cagcatcgaa tttggtacga catccagcgt gaaattcctg tatgtcgcct ggccggctaa
    40321 ctggcaatcc ctatgaaaga tttcatcacc gaagcatggc taagagcgaa ccatacgctc
    40381 agcgaaggcg cagagatcca tctccctgcg gacagtcgcc tgacgccctc tgcccgggag
    40441 ttgctggaaa gccgccatct gcgcatcaag tttattgacg agcagggccg cctgtttgtt
    40501 gacgatgaac agcagcagcc gcagcccgtt catgggctga ccagtagcga tgaacatccg
    40561 caggcgtgct gcgaactgtg ccgccagccg gtggcaaaaa agccggatac gctgactcac
    40621 ctgtcggcgg aaaaaatggt cgccaaaagc gatccgcgtc tgggttttcg cgcggttctc
    40681 gacagcacca ttgcgctggc agtgtggctg caaattgaac tggcggaacc gtggcagccg
    40741 tggctggcgg atatccgttc gcgtcttggc aacattatgc gcgccgatgc gctgggggaa
    40801 ccgctgggct gccaggcgat tgtcgggctt tctgacgaag atctgcatcg actttctcac
    40861 cagccgctgc gctatctcga ccacgatcat ctggtgccgg aagccagcca cgggcgcgat
    40921 gcggcgttgc tcaatctgct acgtaccaaa gtgcgggaaa ccgaaaccgt agcggcgcag
    40981 gtgtttatca cccgcagctt tgaagtatta cgcccggaca tcttgcaggc gctgaaccgc
    41041 ctttcgagca cggtctacgt aatgatgatc ctgagcgtaa cgaagccgcc gctaacggtg
    41101 aagcagatcc aacaacgact gggggaaacg caatgattat tgaacgttgt cgtgaactgg
    41161 cgttgcgagc gcccgccaga gtggtttttc cggatgcgtt agatcaacgt gtgctgaaag
    41221 ctgcgcaata tttacatcaa caaggtctgg caacgcccat tctggtcgct aatccgtttg
    41281 aacttcgtca gtttgcgctc agtcacggcg tggcgatgga cgggctacag gtgattgatc
    41341 cgcatggcaa cctcgcaatg cgggaagaat ttgctcatcg ctggctggcc cgcgcgggcg
    41401 aaaaaacgcc gccggatgcg ctggaaaaac ttaccgatcc gctgatgttc gccgcagcaa
    41461 tggtcagcgc cggtaaagcg gatgtctgta tcgcgggcaa cctctcttcc acggcgaatg
    41521 tgctgcgtgc cggattacgt attatcggct tgcagccagg ctgtaaaacg ctctcatcca
    41581 ttttcctgat gctgccacag tacagcggtc cggcgttggg ctttgccgat tgcagcgtgg
    41641 tgccacagcc gacggcggcg caactggcgg atatcgcgct tgccagtgcc gaaacctggc
    41701 gcgccctcac cggagaagag ccgcgcgtgg cgatgctgtc gttttccagt aacggtagcg
    41761 cccgtcaccc ctgcgttgcc aacgtccagc aggcgacaga aatcgtccgt gagcgcgcac
    41821 caaagctggt ggttgatggc gagttgcagt ttgacgccgc cttcgtgccg gaagtggcgg
    41881 cgcaaaaagc gcctgccagc ccgctacagg gcaaagccaa tgtgatggtt tttccgtcgc
    41941 tggaagccgg aaatattggc tacaaaatcg cacaacgact cggcggatat cgtgccgtcg
    42001 ggccattgat acaaggactt gccgcgccga tgcacgatct ctctcgtggt tgtagcgtgc
    42061 aggaaattat cgagctggcg ctggtggcag ctgtgccgcg tcagacagaa gtgaaccgcg
    42121 aaagcagttt acaaacactg gttgaatgaa cggtcccgtt ctggaccctg ataaagagga
    42181 aagcacaatg gaagcattag gaatgatcga aacccggggc ctggttgcac tgattgaggc
    42241 ttctgacgcg atggttaaag ctgcgcgcgt caagctggtt ggtgttaagc agattggcgg
    42301 cggcctgtgt actgccatgg ttcgtggtga tgttgcagcc tgtaaagccg cgaccgatgc
    42361 aggtgccgca gcggcacagc gtatcggcga gctggtatct gtacacgtta tcccgcgtcc
    42421 gcacggcgac ctggaagaag tcttcccgat cggcctgaaa ggcgatagca gcaacctgta
    42481 atcaccctgt gtgacgtcga ttcgggcgtc acgctctctc ctttttcatt ttaccttctg
    42541 cggagggtag ggggagcttt tacctgaaat acgccacgga ggcgggtatg aaactggcag
    42601 tcgtcactgg acaaattgtt tgtaccgtac gccatcacgg actggcgcat gacaaattgc
    42661 tgatggtgga aatgattgat ccacaaggta atcctgacgg gcaatgcgcc gtcgccatcg
    42721 acaatattgg cgcgggaacc ggggagtggg tgttactggt gagtggcagt tccgcccgcc
    42781 aggcgcataa aagcgaaacg tcaccggtcg atctgtgcgt gattggcatt gtcgatgagg
    42841 tggtgtctgg cggtcaggta attttccaca aataaggcag aacatcatga atcaacagga
    42901 tattgaacag gtggtgaaag cggtactgct gaaaatgcaa agcagtgaca cgccgcccgc
    42961 cgccgttcat gagatgggcg ttttcgcgtc cctggatgac gccgttgcgg cagccaaagt
    43021 cgcccagcaa gggttaaaaa gcgtggcaat gcgccagtta gccattgctg ccattcgtga
    43081 ggcaggcgaa aaacacgcca gagatttagc ggaacttgcc gtcagtgaaa ccggcatggg
    43141 ccgcgttgaa gataaatttg ccaaaaacgt cgctcaggcg cgcggcacgc caggcgttga
    43201 gtgtctctct ccgcaggtgc tgaccggcga caacggcctg actttaattg aaaacgcgcc
    43261 ctggggcgtg gtggcttcgg taactccgtc cactaacccg gcggcaaccg taattaataa
    43321 cgccatcagc ctgattgccg cgggcaacag cgtcattttt gccccgcatc cggcggcgaa
    43381 aaaagtctcc cagcgggcga tcacgctgct caaccaggcg attgttgccg caggcgggcc
    43441 ggaaaactta ctggttactg tggcaaatcc ggatatcgaa accgcgcagc gtttgttcaa
    43501 gtttccgggc atcggccttc tggtggtgac cggcggcgaa gcggtagtag aagcggcgcg
    43561 taaacacacc aataaacgtc tgattgccgc aggcgctggc aacccgccgg tagtggtgga
    43621 tgaaaccgcc gacctcgccc gtgccgctca gtccatcgtc aaaggcgctt ctttcgataa
    43681 caacatcatt tgtgccgacg aaaaggtact gattgttgtt gatagcgtag ccgatgaact
    43741 gatgcgtctg atggaaggcc agcacgcggt gaaactgacc gcagaacagg cgcagcagct
    43801 gcaaccggtg ttgctgaaaa atatcgacga gcgcggaaaa ggcaccgtca gccgtgactg
    43861 ggttggtcgc gatgcggcga aaatcgcggc ggcaatcggc ctgaacgtgc cgcaagaaac
    43921 gcgtttgctg tttgtggaaa ccactgcaga acatccgttt gccgtgaccg aactgatgat
    43981 gccggtgctg cccgtcgtgc gcgtcgccaa cgtggcggat gccattgcgc tggcggtgaa
    44041 actggaaggc ggttgccacc acacggcggc aatgcactcg cgcaacatcg aaaacatgaa
    44101 ccagatggcg aatgctattg ataccagcat tttcgttaag aacggaccgc gcattgccgg
    44161 gctggggctg ggcggggaag gctggaccac catgaccatc accacgccaa ccggtgaagg
    44221 ggtaactagc gtgcgtacgt ttgtccgtct gcgtcgctgt gtattagtcg atgcgtttcg
    44281 cattgtttaa ggagtaacag atggcgcacg acgaacaatg gctcacccca cgtctgcaaa
    44341 cggcggcaac gctgtgtaac cagacgcccg ccgcgacgga atctccgctg tggctggggg
    44401 tggatctggg cacctgcgat gtggtgtcga tggttgtcga ccgcgacggt cagtcggtgg
    44461 cggtatgcct cgactgggcc gatgtggtcc gcgacggcat cgtctgggat ttcttcggcg
    44521 ctgtcaccat tgttcgtcgc catctcgaca cgctcgagca gcaattcggt cgccgtttta
    44581 gccatgcggc gacttcattt cctccaggta cggacccgcg tatttccatc aacgtgctgg
    44641 agtctgctgg actggaagtg agccatgtgc tggatgagcc aaccgcagtt gcggatctgc
    44701 tgcaactgga caacgccggt gtagtggata tcggcggcgg cactaccggc attgccatcg
    44761 tgaaaaaggg caaggtgacg tactcggcgg atgaagcaac cggcggacat cacatctctc
    44821 tgactctcgc cggaaatcgc cgtatttcgc tggaagaggc ggagcagtac aagcgcggtc
    44881 acggtgaaga gatttggcct acggtgaaac cggtgtacga aaaaatggcg gacattgtcg
    44941 cccggcatat tgaagggcag ggcattactg atttatggct ggcgggcggc tcttgtatgc
    45001 aaccgggcgt ggcggaactg tttcgcaaac aattcccggc gttacaggtg catttaccgc
    45061 agcacagttt gtttatgacc ccgctggcga tcgccagtag tgggcgagag aaagcggagg
    45121 ggctatatgc aaaatgaatt gcagaccgcg ctctttcagg cgttcgatac cctgaatctg
    45181 caacgggtaa aaacatttag cgttccaccg gtgacgcttt gcggtccggg ctcggtgagc
    45241 agttgcggac agcaagcgca aacgcgtggg ctgaaacatc tgttcgtgat ggcagacagc
    45301 tttttgcatc aggcagggat gaccgccggg ctgacgcgca gcctggctgt taaaggcatc
    45361 gccatgacgc tctggccatg tccggtgggc gaaccgtgca tcaccgacgt gtgtgcagcc
    45421 gtggcgcagt tgcgtgagtc aggctgtgat ggggtgatcg catttggcgg cggctcggtg
    45481 ctggatgcgg cgaaagccgt ggcgttgctg gtgacgaacc cggatagcac gctggcagag
    45541 atgtcagaaa ccagcgttct gcaaccgcgc ttgccgctga ttgccattcc aacgaccgcc
    45601 ggaaccggct ctgaaaccac caatgtaacg gtgattatcg acgcggtgag cgggcgcaag
    45661 caggtgttag cccatgcctc gctgatgccg gatgtggcga ttctcgacgc cgcgttgacc
    45721 gaaggtgtgc cgtcgcatgt cacggcgatg accggcattg atgcgttaac ccatgccatt
    45781 gaagcataca gcgccctgaa cgctacaccg tttaccgaca gtctggcgat tggtgccatt
    45841 gcgatgattg gcaaatcgct gccgaaagcg gtgggctacg gtcacgacct tgccgcgcgc
    45901 gagagcatgt tgctggcttc atgtatggcg ggaatggcgt tttccagtgc gggtcttggg
    45961 ttgtgccacg cgatggcgca tcagccgggc gcggcgctgc atattccaca cggtctggcg
    46021 aacgccatgt tgctgccaac ggtgatggaa tttaaccgga tggtttgtcg tgaacgcttt
    46081 agtcagattg gtcgggcact gcgaactaaa aaatccgacg atcgtgacgc tattaacgcg
    46141 gtaagtgagc tgattgcgga agttgggatt ggtaaacgac tgggcgatgt tggtgcgaca
    46201 tctgcgcatt acggcgcatg ggcgcaggcc gcgctggaag atatttgtct gcgcagtaac
    46261 ccgcgtaccg ccagcctgga acagattgtc ggactgtacg cggcggcgca ataaatgccg
    46321 gatgcgacgc ttgccgcgtc ttatccagcc tacgggattg cacatgtagg gcggataagg
    46381 cgttcacgcc gcatccgcca gtggcgcggt gcaattgccg gatgcgatgc ttgccgcgtc
    46441 ttatccggcc tacgggattg cacatgtagg gcggataagg cgttcacgcc gcatccgcca
    46501 gtggcgcggt gcaattgccg gatgcgacgc ttgccgcgtc tgatccggcc ttcgggattg
    46561 cacatgtagg gcggataagg cgtttacgcc gcatccgcca ataaataaca actaacaggg
    46621 agtaaaggcg atgggaatta acgaaatcat catgtacatc atgatgttct ttatgctgat
    46681 agctgccgta gacaggatcc tgtcgcagtt cggcggttct gctcgtttcc tcggtaagtt
    46741 cggtaaaagt atcgaaggat caggcggtca gttcgaagaa ggctttatgg caatgggcgc
    46801 tctgggtctg gcgatggtcg gtatgaccgc gctggcaccg gtactggctc acgtactcgg
    46861 gccggtaatt atcccggttt acgaaatgct cggcgcaaac ccgtcaatgt tcgccggaac
    46921 gctgctggcg tgcgatatgg gcggcttctt cctcgccaaa gagctggcgg gcggcgacgt
    46981 agcagcgtgg ctatactctg ggttaattct tgggtcgatg atggggccaa cgattgtgtt
    47041 ttccattccg gtggcgctcg gcattatcga accttctgac cgtcgttatc tggcgctcgg
    47101 cgtgctggcg ggcattgtga ccattccgat tggctgtatt gctggtggtc tggttgctat
    47161 gtactccggt gtgcagatca acggtcagcc ggtggaattc actttcgccc tgatcctgat
    47221 gaacatgatc ccggtgatca ttgttgcgat tctggtggcg ctggggctga aattcatccc
    47281 ggaaaaaatg atcaacggct tccagatctt cgccaaattc ctcgttgcat tgatcaccct
    47341 cggtcttgct gccgcggtag ttaagtttct gcttggctgg gaactgatcc ccggtctgga
    47401 ccctatcttt atggcccctg gcgataaacc cggtgaagtg atgcgcgcca ttgaagttat
    47461 cggttctatc tcctgcgttc tgttaggggc gtatccaatg gtgctgctgc tgacccgctg
    47521 gtttgaaaaa ccgctgatga gcgtcggtaa agtgctgaat atgaacaaca tcgcggcagc
    47581 cggcatggtg gcaacgcttg ccaacaacat cccgatgttc ggcatgatga agcagatgga
    47641 tacccgcggc aaagtcatca actgcgcctt cgccgtttcc gctgctttcg ccctgggcga
    47701 ccacttaggc ttcgccgctg ccaacatgaa cgccatgatc ttcccgatga ttgtcggcaa
    47761 gttgatcggc ggcgtaacgg cgattggcgt ggcgatgatg ctggtgccaa aagaagacgc
    47821 gaccacggct aaaaccgaag cggaggcaca atcgtgaaca ctcgccagct attgagcgtc
    47881 ggtatcgata tcggcaccac caccacccag gtgattttct cccacctgga gctggttaac
    47941 cgtgcggcgg tgtcgcaggt gccgcgctac gaattcatta aacgcgaaat tagctggcaa
    48001 agcccggtgt tctttacccc tgtcgataaa cagggtggtt taaaagaagc ggaactgaaa
    48061 accttaatcc tcgagcaata tcaggctgcg ggcattgcgc cggaaagcgt tgattctggt
    48121 gccatcatca tcaccggtga aagcgcgaaa acccgcaatg ctcgtccggc ggtgatggtg
    48181 ctctcccagt cgctgggcga ttttgtcgtt gccagcgccg ggccgcacct cgaatccgtc
    48241 attgccggtc acggtgccgg ggcacaaacc ctttctgaac aacggctgtg tcgggtactg
    48301 aatattgaca tcggcggtgg cactgcgaac tacgccctgt tcgatgccgg aaaaatcagc
    48361 ggcactgcct gcctcaacgt cggtggtcgc ctgctggaaa ccgacagcca ggggcgcgtg
    48421 gtttacgctc ataaaccggg gcagatgatt gtggatgagt gcttcggcgc aggcaccgac
    48481 gcccgttcgc tgaccggcgc gcagctggtg caggttaccc ggcggatggc agaactgatt
    48541 gtcgaagtga ttgacggaac gctctcgccg ctcgcgcagg cattgatgca aaccggtttg
    48601 ctgcccgcag gtgttacgcc cgaaatcatt acgctttctg gcggcgtggg cgaatgttat
    48661 cgccaccagc ccgccgaccc gttctgtttt gccgatattg gcccgctgct ggcaacggcg
    48721 ctgcatgacc atccgcgcct gcgtgagatg aacgtacagt ttccggcgca aaccgtgcgc
    48781 gccacggtga ttggcgcggg cgcgcatacc ctttcgctct ctggcagcac aatctggctt
    48841 gagggcgtac aactgccgct gcgcaatttg ccggtggcga tcccgattga tgaaacggat
    48901 ctggtgagtg cctggcaaca ggcgctgctt cagctggatc ttgatcccaa aactgacgcg
    48961 tacgtgctgg cgcttcccgc ctcgctgcct gtgcgttacg ccgcggtact gacggtcatc
    49021 aacgcgctgg tcgatttcgt cgcgcgtttt ccaaatccgc atcccctgct ggtggtggcc
    49081 gggcaggact ttggtaaagc tctgggcatg ttgttgcgcc cacagctaca acaactcccg
    49141 ttggcagtca ttgacgaagt gattgtccgc gcgggggact atatcgacat tggtacgcct
    49201 ctttttggcg gatcggttgt gccggtgacg gtgaaatcac tcgcatttcc ttcctgaggg
    49261 aacgacttat gaaactaaag accacattgt tcggcaatgt atatcagttt aaggatgtaa
    49321 aagaggtgct ggctaaagcc aacgaactgc gttcggggga tgtgctggcg ggcgttgcag
    49381 cggcaagctc acaggagcgc gtggcggcaa agcaggtgtt gtcggaaatg accgtagcgg
    49441 acatccgcaa taatccggtg attgcctatg aagatgactg cgtgacgcgg ctgattcagg
    49501 acgatgttaa cgaaacggcc tacaaccaga ttaaaaacag gagcatcagc gaactgcgtg
    49561 agtatgtgct gagcgatgaa accagcgtgg acgacattgc ctttacccgc aaagggctga
    49621 cctcggaagt ggtcgcggcg gtagcgaaga tttgctccaa cgcggacctg atctacggcg
    49681 cgaagaaaat gccggtaatc aaaaaggcca ataccaccat cggtattccg ggcaccttca
    49741 gcgcccgttt gcagccaaat gacacccgtg acgacgtgca aagtatcgct gcacaaatct
    49801 acgaagggct ttccttcggg gtgggcgatg cggtgatcgg cgttaacccg gtaactgacg
    49861 acgtggaaaa cttaagccgc gtgttggaca ccatttacgg cgtgatcgac aaattcaaca
    49921 tcccaactca gggctgcgta ctggcgcacg tcaccaccca gatcgaagcg attcgtcgcg
    49981 gcgcgcctgg cggactgatt ttccagagta tctgtggcag cgaaaaaggg ctgaaagagt
    50041 ttggcgtgga actggcgatg ctcgacgaag cgcgcgcagt gggtgctgag ttcaaccgta
    50101 tcgccgggga aaactgcctc tacttcgaaa ccgggcaagg ttcggcgctg tctgctggcg
    50161 ctaacttcgg cgcagaccag gtgacgatgg aagcacgtaa ctatgggctg gcgcgtcatt
    50221 acgatccgtt tatcgtcaac accgtggtcg gctttattgg gccggagtat ctctacaacg
    50281 accgccagat tatccgtgct ggcttagaag atcactttat gggcaagctg agcggcatct
    50341 ctatgggctg tgactgctgt tacaccaacc acgctgacgc tgaccagaac ctcaacgaaa
    50401 acctgatgat cctgctcgcc accgcaggct gcaactacat catggggatg ccgctgggcg
    50461 atgacatcat gctcaactat cagaccaccg cattccacga cactgccact gtgcgtcagt
    50521 tactcaacct gcgcccgtca ccggagtttg aacgctggct ggaaagcatg ggcattatgg
    50581 caaacggtcg cctgaccaaa cgggcgggcg atccgtcact gttcttctga tgacgcggag
    50641 ataacacatc atggatcaaa aacagattga agaaattgta cgcagcgtga tggcgtcaat
    50701 gggacaagcg gcccccgcgc cgtcagaagc aaagtgcgcc accactaact gtgcggcacc
    50761 ggtgacctcg gaaagctgcg cgctggattt aggttctgcg gaagcaaaag cgtggattgg
    50821 tgttgaaaac ccgcatcgcg cagacgtatt aacagaactg cgccgcagca ccgtggcccg
    50881 cgtttgtacc ggtcgtaccg gtccgcgtcc acgtacgcag gcgctgctgc gtttcctggc
    50941 cgatcactct cgttcgaaag ataccgtact gaaagaagtg ccggaagagt gggtgaaagc
    51001 gcagggcttg ctggaagtac gctccgaaat tagcgacaaa aatctctacc tgactcgccc
    51061 ggatatgggc cgccgcctgt gtgcagaagc tgttgaagcg ctgaaagcac agtgtgttgc
    51121 taatccggac gtacaggtcg ttatttctga tggcctgtca actgatgcca tcaccgtgaa
    51181 ctacgaagag atcctgccgc cgctgatggc gggcctgaaa caggccgggc tgaaagtcgg
    51241 cacgccattc tttgttcgtt atggtcgcgt gaagattgaa gatcagatcg gtgagatcct
    51301 cggcgcgaaa gtggtgatcc tgctggtggg ggaacgtccg gggttgggtc agtcagaaag
    51361 cctctcctgt tacgccgttt actcgccgcg tattgcgacc accgttgagg ccgatcgcac
    51421 ctgcatctct aacattcacc agggcggcac gccgccggtt gaagccgcag ccgtcattgt
    51481 ggatttggcg aaacgtatgc tggagcagaa agcatccggc atcaacatga cccgataagg
    51541 aggcatcatg ccagctttag atttgattcg accgtcggta accgccatgc gggtgattgc
    51601 ctccgttaac gctgaatttg cgcgtgaact aaaattgccg ccgcatattc gtagcctcgg
    51661 gctgatttct gctgattccg atgacgtcac ctacattgcg gctgacgaag cgaccaagca
    51721 ggcgatggtc gaagtggtat atggccgctc gctgtatgcg ggagcagcac acggaccgtc
    51781 accgactgcc ggtgaagtgc tgattatgct tggtgggcca aacccggcgg aagtgcgtgc
    51841 tggtctggat gcgatggttg cgcatattga aaatggcgct gctttccagt gggctaatga
    51901 cgcagaaaac accgcattcc tggcacatgt agtttcgcgt accggttctt atctttcatc
    51961 aaccgccggg atcacgcttg gcgatccgat ggcgtatctg gtggcaccgc cgctggaagc
    52021 gacctacggc attgatgcag cgttgaaatc tgccgacgta cagctggtga cctatgtccc
    52081 gccaccgtct gaaaccaact actcggcagc atttttaacc ggtagccagg ccgcgtgtaa
    52141 agcagcctgt aacgccttta ccgatgcagt gctggaaatc gcgcgtaatc caatccagcg
    52201 tgcgtaacgg aggttgccga tgatcaatgc actgggattg ctggaagtgg acggcatggt
    52261 cgccgcgata gatgcagcgg atgccatgct caaagcagct aacgttcgtc tgctcagtca
    52321 cgaagtgctt gaccctggtc gcttaacgct ggtggtggaa ggcgatctgg cggcgtgtcg
    52381 tgcggcgctg gacgcaggtt gtgctgccgc gatgcgtacc gggcgtgtca tcagccgcaa
    52441 ggagatcggt cggccagacg atgacaccca gtggctggtt actggcttta accgccagcc
    52501 gaagcaaccc gtaaaggaac ccgacgcgcc agttatcgtc gcggaatctg ctgacgagtt
    52561 gttggcgctg ttaacatcag tacgtcaggg aatgacggca ggagaagtgg ctgcccactt
    52621 tggctggccg ctggaaaaag ccagaaatgc gctcgaacag ctcttttctg ccgggacgtt
    52681 acgtaaacgc agtagtcgtt atcgtctcaa gccccattaa cctgtcggag gtgccgggtg
    52741 tcctacaaca cccggcatta acatcatgaa aaagacccgt acagccaatt tgcaccatct
    52801 ttatcatgaa cccttacccg aaaacctgaa gctcacgccg aaggtcgaag tggataatgt
    52861 tcatcaacga cagacaacgg atgtctatga acatgctttg acgattaccg cctggcagca
    52921 gatttacgat cagctgcatc cgggcaagtt tcatggtgaa tttacggaaa ttctgctcga
    52981 tgatattcag gtttttcgtg aatacaccgg tctggcgctg cgtcagtcgt gcctggtctg
    53041 gccgaactcg ttctggtttg gcattccggc gacgcgcggt gagcagggat ttatcggttc
    53101 gcaatgtctg ggaagcgcag aaatagcgac gcgccccggt ggaaccgagt ttgaattaag
    53161 tacgccggac gattatacga tcctgggcgt agtgctgtct gaagatgtca tcacccgtca
    53221 ggctaacttt ttgcacaatc cggatcgggt attacatatg ctgcgtagcc agtcggcgct
    53281 ggaagtgaaa gagcagcata aagccgcgct gtggggcttt gtccaacagg cgctggcgac
    53341 gttttgcgag aacccggaaa atctccatca gcctgcagtg cgaaaagtgc tgggggataa
    53401 tttgctaatg gcgatggggg cgatgctgga agacgcgcaa ccaatggtga cggcggaaag
    53461 catcagtcat cagagttacc gtagattact ttcccgcgcc cgtgaatatg tgctggaaaa
    53521 tatgtctgag ctggtgacgg tgctggactt gtgtaatcaa ctgcatgtca gtcgccgcac
    53581 gctacaaaac gcgtttcacg ctattttagg cattggccca aacgcgtggc tgaaacgcat
    53641 tcgcctgaac gccgtacgcc gcgaactgat aagcccgtgg tcgcaaagca caacggtaaa
    53701 agacgccgcc atgcagtggg gattctggca tctggggcaa tttgccacgg attaccagca
    53761 gctgtttgcc gagaagccgt cgttgacgtt gcatcagcgg atgcgggaat gggggtgagg
    53821 gagttacacc caatccctga ccttaataaa ctcacttaac gccgcttctg ggctgccatc
    53881 ttttggctga taatcatact cccagcgtac cagtggcggc attgacatca ggatcgactc
    53941 ggtgcgcccg ccagtttgca ggccaaacag agtgccgcga tcccagacca gattgaactc
    54001 gacataacga ccgcgacggt agagctgaaa attgcgctcg cgctcgccgt aggccatcgc
    54061 tttacgtcgc tctacaattg gtaaataagc gtcggtgtag cctttgccta ccgcctgcat
    54121 aaaggcaaaa cagtggtcga aatctggcgt gttcagatca tcaaagaaca gcccgccaat
    54181 accgcgctgt tcgttgcgat gtttgaggta gaagtaatcg tcgcaccact ttttgtaacg
    54241 gggataaacg tcttcaccaa atggcaggca caggtcacgg gcggtgcggt gccagtgaat
    54301 ggcgtcttct tcaaaaccat agaaaggggt taaatcgaag ccgccgccaa accaccagac
    54361 gggctctgca cccggttttt cggcaataaa aaaacgcaca ttcgcgtggc tggtgggaac
    54421 atacgggtta tgcggatgca ctaccagtga aacgcccatc gcctcgaaac tgcgcccggc
    54481 aagttccggg cgatgagcgg tggcggaagc aggcatcgcc tcgccgtgga cgtgagaaaa
    54541 gttgacgcct gcctgttcga aaacaccacc attacgcaac acccgactac gcccgccgcc
    54601 gccagcttcg cgctgccaac tatcttcgac aaattctgcg ccatcgacgg cggtcagctg
    54661 ctgacaaatc gtatcctgaa ggttgagcag aaactgttta acctggtgtg cgtcgggttt
    54721 cataacttat cgctttttcg aatgtgcttt ctggttgtcg aaccagtgga aataactgat
    54781 cacgccttca gcaatcgctg tggcgatttt ctgacgaaac gccgccgtgc ctaacagccg
    54841 ctcttcttcc gggttggtga taaacgaggt ttccaccagc accgaaggaa ccgacggcga
    54901 tttcaacacc acaaacgcgg cttgttcggt gttgcggctg tgcagtttat gcaccggctt
    54961 aatctttttc agaatatgcg agccaagcgt caggctgttt ttaatagtat cggtttgcac
    55021 cagatcaaac agcacttgct gcaataggtg atccttgtca gtcgcctttt taccggcaac
    55081 ttcatcggcg cggttttcgc gttcagacag gtatttcgcc attgcgctac ttgccccacg
    55141 gttagagagg gcaaataccg aagcaccggc agctttcggg ttggtaaagc catcggcatg
    55201 aattgacata aacagatctg cgccatgttt atgggcgatt tcaacgcgat cgtaaagtgg
    55261 gataaacgta tcgccagaac gcgttaaacg cgcatcaatc ccatgattac gcaaaatgga
    55321 acggacgttt ttagcaatcg ccagcaccac atgtttttct ttcgaaccgt tgcgtccgat
    55381 cgctccggta tcaattccgc cgtggcctgg atcgagaaca acgacacgtt tgccgccaga
    55441 ttttttggct ttcggcttgc tgtgtccgtt gctggttttt aaaggttcgt ctttggcgat
    55501 ggcttgcgac attcctggca acgtcagggc agccaaaccg gctttcagca cctggcggcg
    55561 cgaagtgagt gtttttagtg gtttaaaagt gctcatacgg cctgagttgt aataataaag
    55621 ttccagatgt tatatcgtat cgcgtaatcc gttacgacgg tttgatgttg agaattgttt
    55681 tacttttcat ttcaatagtt gacaactccc cattatgcca ctttttcaac cgattttcgt
    55741 cagatattgg ctaattcgac cgttcacgcc ataatcagcc ttttaacccg caaaaaggta
    55801 acgaatacca tggagatacg cgtatttcgc caggaagatt tcgaagaggt catcaccctt
    55861 tgggagcgtt gcgacttgct gcgtccgtgg aacgatccgg aaatggacat cgagcgtaag
    55921 atgaaccatg acgtcagttt gtttttggtc gctgaggtaa acggtgaagt ggtcggaacg
    55981 gtgatgggcg gttatgacgg gcatcgcggg tctgcttatt atcttggcgt gcatccagag
    56041 tttcgtgggc gtgggattgc caatgcgttg cttaatcggc tggagaaaaa gctgattgct
    56101 cgtggctgcc cgaaaattca gatcaatgtg ccggaagata acgacatggt gctcggaatg
    56161 tatgaacgcc tgggatatga acacgccgac gtgctgagtc tgggtaagcg tttgattgaa
    56221 gatgaagagt actgagttcc atcctgtcca ttatgatgcg cacggacgcc tgcgtttacc
    56281 cttgctcttc tggcttgtgc tattgcttca ggcgcgaacc tgggtgctgt ttgtcattgc
    56341 cggtgcgtcg cgcgagcagg gtactgcgct gctaaatctg ttttatcccg atcacgataa
    56401 tttctggctg gggctgattc caggcattcc ggcggtgctg gcgtttttgt tgagcggtcg
    56461 gcgggctaca tttcctcgca cttggcatgt gctctatttt ctgttgctgt tggcgcaggt
    56521 ggttttactt tgctggcaac cgtggctgtg gctgaacggt gaatccgtta gcggtatcgg
    56581 tctggcgctg gtagtggcgg atattgtggc gttaatctgg ctgctgacca atcgacgttt
    56641 acgcgcttgt tttaatgaag aaaaagaata acggcacttt ttggtgaatt tgcactccaa
    56701 gcaacgttat tgaataacca aaggaagtga catgaaatcg ctgcgtttaa tgttatgcgc
    56761 tatgccgttg atgctgaccg gctgttccac gatgtcgtca gttaactggt cggccgctaa
    56821 cccgtggaac tggtttggat cgtccaccaa agtgagcgag cagggcgtgg gtgaattaac
    56881 ggcgtccaca ccactgcaag aacaagccat tgctgatgcg cttgatggcg attatcgcct
    56941 gcgcagcgga atgaaaaccg cgaacggcaa cgtggtgcgc ttttttgaag tgatgaaagg
    57001 cgacaacgtg gcgatggtga ttaacggcga tcaaggcacg atcagtcgca ttgacgtgct
    57061 ggatagcgat attcctgctg acaccggtgt taaaatcggt acaccgttta gcgaccttta
    57121 cagcaaagca tttggcaatt gccaaaaagc tgatggtgat gataatcgtg ccgtcgaatg
    57181 taaagccgaa ggcagtcaac atattagcta ccagttcagc ggggaatggc gtggtcctga
    57241 agggttaatg ccttcggacg ataccctgaa aaactggaaa gtcagtaaaa ttatctggcg
    57301 gcgttaattt gcgtctgaac aaaccgccgc cgcgaaaaaa cacgtaaaat agcgctcaac
    57361 aatgccacgg attgcgtggc attcttattt tcaggaggaa caatgtctca ggttcagagt
    57421 ggcattttgc cagaacattg ccgcgcggcg atttggatcg aagccaacgt gaaaggggaa
    57481 gttgacgccc tgcgtgcggc cagtaaaaca tttgccgaca aactggcaac ttttgaagcg
    57541 aaattcccgg acgcgcatct tggtgcggtg gttgcctttg gtaacaacac ctggcgcgct
    57601 ctgagcggcg gcgttggggc agaagagctg aaagattttc cgggctacgg taaaggcctt
    57661 gcgccgacca cccagttcga tgtgttgatc cacattcttt ctctgcgtca cgacgtaaac
    57721 ttctctgtcg cccaggcggc gatggaagcc tttggtgact gcattgaagt gaaagaagag
    57781 atccacggct tccgttgggt ggaagagcgt gacctgagcg gctttgttga cggtacagaa
    57841 aacccggcgg gtgaagagac gcgtcgcgaa gtggcggtta tcaaagacgg cgtggatgcc
    57901 ggcggcagct atgtgtttgt gcagcgttgg gagcacaacc tgaagcagct caaccggatg
    57961 agcgttcacg atcaggagat gatgatcggg cgcaccaaag aggccaacga agagattgac
    58021 ggcgacgaac gtccggaaac ctctcacctc acccgcgttg atctgaaaga agatggcaaa
    58081 gggctgaaga ttgttcgcca gagcctgccg tacggcaccg ccagtggcac tcacggtctg
    58141 tacttctgcg cctactgcgc gcgtctgcat aacattgagc agcaactgct gagcatgttt
    58201 ggcgataccg atggtaagcg tgatgcgatg ttgcgtttca ccaaaccggt aaccggcggc
    58261 tattacttcg cgccgtcgct ggacaagtta atggcgttgt aagcgtttct gattcgatga
    58321 ttaaaggcca gccagtaaca aaactgactg gcctttttat tacttttgct tcaacgccgc
    58381 atacacctga tcaactaccc aaccataagt tgcaatcggt aactgaccgc tttcgaacat
    58441 attgggattt ttttgtggat cggcaactgg cgaatggggc ttgttgctta acatcacaat
    58501 cgccatatga ttgaccggat cgataacggt tactgtcccg gtccagccag tgtgaccgta
    58561 ggtttgcggg cttgccagcg tgccaaacgt cggcgtcatg gtggcattac cattcacgcg
    58621 ccagccgagg ccaaaagtgg catcttcctt agagctggtg gtgaacatct tcaccgtttc
    58681 cgcactgaac agctgcacat cgccatagcc cccgccgttc agcatcgttt gcattaacac
    58741 cgcaatatcg ccggtattgg aaaacaaacc tgcgtgcccg gaaacgccgc ccatcgaata
    58801 aaaggctttt tcatcgtgca cctgacccca gagagtggag gtgcggatat tcgggaaatg
    58861 gatcacgcca tcgcgggtat tgccgttcaa ttccgtggcg gcaatttgtt gcggtttaaa
    58921 gcctttcagc agcgggttaa ataccgtatg tgtcaggccg agcgggcgat aaatcgactc
    58981 ttcaacatag cggtcaagcg gctgaccggt aaccgactcg acgataaatc caagcagcat
    59041 ataatcgaca tcgctgtaga tatgtttgct gccgggctga tattccagcg gcgtgcgctt
    59101 gatcatctcc agcgtctggc ctttatcctg ggaatataac gcgcccgcga ccgctttatt
    59161 cgggtattgc ggatctgccg ggaaaccgcc gctgtgatgc agcaggtcag aaatccgcag
    59221 ggtgtttttg cctttaatgg tgtcatttgg actatcggca aaccccggaa tatatttcgc
    59281 aatccgatcg tcaggatgca gtttgccttc gctcatcagc ttttgcaggg cgaagttcgt
    59341 ggcgtacatt ttggtgtttg aggccaggtc atacagcgtc ccggtggtgg ctttgacagg
    59401 ctgttccatc aacacgctgc catcgtactt tttcgccgct ccccaggctt tgcgatacac
    59461 aatctgatta tctttaatga tcagcaggtt tacgccggga taaccgacat caatttgctg
    59521 gctaatccag cgatccatct ggttaagccg ttcgacgtta aaccctgctt tctctggcga
    59581 gctttctgtc agaacagggt attttgcggc gctgacgcta caactaacag ccagcagaga
    59641 aagatagagc attgtccgtt tcatgatatc agtccagatt gacgttacgg cagccaaaga
    59701 gcgtggtgaa aataaacccg caaacccatg ccaccagaat cccgccagca taaaccgcca
    59761 tggccggaag gatgccttgt gcggaagtca tcagcggcag cgctaccaga ccagacggcc
    59821 caaaggcgct gtttaagccc atcggcagac cccatcaggc tatcaggccg ataaacaaac
    59881 cgcccgccgc gccgcctaaa caggcggtaa caaacggctt catgcggggc agggtgacac
    59941 cgtaaatcag cggttcacca acgcccagca ggccgggaat aatcgccccg cgtacctgac
    60001 tgcgtagcgc actgtgcggt tgcgcccgcc agtagagtgc cagcgccgcg cccacctggc
    60061 ccgcgcctgc cattgaaagg atgggaaata agctgttgaa tccctggctg tccattaacg
    60121 cgaggtaaac aggaataaag ccctgatgca cgccaaatac cacggcgatc aggaacagcc
    60181 cggctaaaac cgcacaaccg aacggattac tgttcaggtg cataaacagc catgacatac
    60241 cttcgaataa ccagccaccc agcggcataa tgatcaggta cgcaagcgta gcggtgatca
    60301 gcagggtgat taacgaggtc agcagcatgt cgagatcgtc cgacataaag cggcgcacca
    60361 tgccttcaat gcgggcgcag gcccacgcgg caatcagcac gccgataata ttgccgcgcg
    60421 gatcgatggg cagaccaaag aaatcgtgaa aaccggcgta gtaaccggtg gttgcagcgg
    60481 ggttataacc gagcaaaaag agtgcggcaa taattgcgcc attgacgccc gtgccgccaa
    60541 atgcctgggc ggcgttatag cccaccagaa tcaccaggaa agtgaacaaa cctttgctga
    60601 acaccttcat aaaattcagc gcgtcgggta gcgtgccctg agcatctgcc ggaacgtgca
    60661 tcaccgtggc aattaacgtc gctatcccca gcagcagacc ggcggcaata aaaccgggga
    60721 tcagcggcgt gaagatggtg gcgaatttgg cgagaaattg ttgcacgccg gaggtttgtt
    60781 tggcttttaa ctgacgtttg ttctgggcgg cgatttctgc ggcatcctgt accggtgcct
    60841 cgcccagcag ctcgctcatc gcttttgcgg cacgatgcgc tttaccaggc ccaaaaacga
    60901 cctgcacctg gtcgctggtc aaaatgacgc ccttcacgcc ttccagcgtt ttgatattgg
    60961 gatcaaccag tgaactgtca tgtacaccca gacgcaggcg cgtcatacag ttaccacaac
    61021 tggcgatatt tcccagtccg ccgacacggg taagaatggt gttcagaagt tcactgctga
    61081 tctctttggc catgggttat tccttgtcta aaacctgacg aataaagccg ccgtgttgat
    61141 ccaggcgttt tttagcttct gcggcatcga gatttttcag caccatcaca atggccgttt
    61201 tacagttgcg ctcgcaggca attaacgccg cttccgcttg ttcggcatta catccggtgg
    61261 cgtttttaac gatattgacc tggcgcacat gcagtttttc gttggtggcg accacatcga
    61321 ccatcaggtt gccgaacact ttgccggatt taatcatcag cccggtggaa agcatattga
    61381 gcaccagttt ctgcgctgtg cctgctttca ttcgcgaaga accagtgacc acttcggcac
    61441 ctacaatcgg tgtaatggca aactcggcgg tggtggaaac ggcactcccc ggattacagg
    61501 aaattcccac tgtgcggcaa ccgagctggc gggcgtattc cagtccggca atcacatacg
    61561 gcgtacgacc gctggcggca ataccgacca ccacatcctg tgccgttaaa tttatatttt
    61621 tcagatcgtt aatgccgcct tctcggctat cttccgtgcc ttccaccgcg tgctgaatgg
    61681 catattcgcc gccagcaatc aaaccaacca ccagacccgg tttcacgccg taggtgggcg
    61741 gacattcgct ggcatccaga atccccagac gaccggatgt tcccgcaccg agataaatca
    61801 gacgaccgcc gccgcttacc tgggcgtgga taacatcgat cgccgcggcg atatccggca
    61861 ggacgcgctc aacggcaagc ggtacggttt tatcttcatc gttgataatc cggcacattt
    61921 ccagcgtcga tacgcggtca atttcagccg aggcggtgtt cgagccttca gtaatcatct
    61981 tttcaagttg catcggtggt gaccttacta tttacgatca aataattgca gatgtatgga
    62041 atcatatatc ccgaattgac agacttaaag gaatcattga gtctacactt gcagagtgtg
    62101 aagagcgtca taagaactta agcggaattt ttgctgccag cccgggagag tggaagatgt
    62161 tgtacctgac gaaaatacgc aatgcggaaa gtgaatttac cgagaacgag caaaaaattg
    62221 ctgactttct gcgggccaac gtgagtgagc tgaaatccgt ctcttcgcgc aagatggcga
    62281 aacagctcgg catcagtcag tcgagcattg tgaaatttgc ccagaagctc ggcgcgcaag
    62341 gatttactga attgcgtatg gcgttaattg gcgaatacag tgccagccgg gagaaaacga
    62401 atgccacggc gctgcacctg cacagttcga ttaccagtga tgattcgctg gaagtgattg
    62461 cccgcaaact gaatcgcgaa aaagagctgg cgctggagca aacctgtgcg ttgttcgact
    62521 acgctcggct gcaaaaaatc attgaggtca tcagtaaagc accgtttatc cagataacgg
    62581 gcctgggcgg gtcggcgctg gtggggcgcg atctgtcgtt caaactgatg aaaattggct
    62641 atcgcgtggc ctatgaagcc gacacacacg tacaagccac cgtttcccag gcgttgaaaa
    62701 aaggcgatgt acaaatcgct atctcttaca gcggcagtaa aaaagagatt gtcttgtgcg
    62761 cagaagcggc acgaaagcag ggggcaacgg tgattgccat tacttctctg gctgactctc
    62821 cgctgcggcg gctggcgcat ttcacgctcg ataccgtttc cggtgaaacc gaatggcgta
    62881 gctcgtcgat gtctacccgc acggcgcaaa attctgttac cgatttactg tttgttggtc
    62941 ttgtgcaact caatgatgtg gagtcattga aaatgattca gcgcagcagt gagctaacac
    63001 aacgcctgaa atgatgcata aagcagcgac tggattgaga ttttcctgaa ttagtgagct
    63061 gatccgcagc aatattttgt ttatcctgta ttttcagagg gaatggagtg taacgctctg
    63121 tattaacaag gagagcatta aaatgggtaa actcacgggc aagacagcac tgattacggg
    63181 cgcattgcag ggaattggcg aaggaattgc cagaactttt gcgcgtcatg gcgcaaacct
    63241 aatcttgctg gatatctccc ctgagatcga aaagctggcg gacgaactgt gtggtcgtgg
    63301 tcatcgctgt acggcggttg tcgccgatgt gcgtgacccg gcgtcggtag ccgcagctat
    63361 caaacgcgcg aaggaaaaag aagggcgcat tgatatcctg gtgaataacg caggcgtttg
    63421 tcgtctgggc agtttcctcg atatgagcga tgacgatcgc gatttccata ttgacatcaa
    63481 tattaaaggc gtatggaacg tcacgaaggc ggtgctgccg gagatgattg cccgcaaaga
    63541 tggtcgcatt gtgatgatgt cttcagtcac tggtgatatg gtggccgatc ctggcgaaac
    63601 ggcgtacgcc ttaacgaaag cggcgattgt tggcctgaca aaatcgctgg cggtggagta
    63661 cgcgcagtct ggtattcgcg ttaacgccat ttgcccggga tacgtgcgca caccaatggc
    63721 ggaaagcatt gcccgccagt cgaacccgga agatccagag tcggtgctga ctgaaatggc
    63781 gaaagcaatc ccgctgcgtc gcctcgccga tccgctggaa gtcggcgaac tggcggcctt
    63841 cctcgcatcg gatgaatcca gctatttaac cggtacacag aatgtgattg atggcggcag
    63901 cacactgccg gagacggtta gcgtcggtat ctgattcacc tctgtttcct ccctgcattt
    63961 gtggggagga tttcgtttta aacccggttc ttattccctt ttcaacttcc aaatcaccaa
    64021 acggtatata aaaccgttac tcctttcacg tccgttataa atatgatggc tattagaaag
    64081 tcattaaatt tataagggtg cgcaatggcc gttaacttac tgaaaaagaa ctcactcgcg
    64141 ctggtcgctt ctctgctgct ggcgggccat gtacaggcaa cggaactgct gaacagttct
    64201 tatgacgtct cccgcgagct gtttgccgcc ctgaacccgc cgtttgagca acaatggacc
    64261 aaagaaaacg gcggcgacaa actgacaata aaacaatctc atgccgggtc atcaaaacag
    64321 gcgctggcaa ttttgcaggg cttaaaagca gacgttgtca cttataacca ggtgactgac
    64381 gtacaaatcc tgcatgacaa aggcaagctg atcccggcgg actggcaaac gcgcctgcca
    64441 aataatagct cgccattcta ctccaccatg ggcttcctgg tgcgtaaggg caacccgaag
    64501 aatatccacg actggaacga cctggtgcgc tccgatgtga agctgatttt cccgaacccg
    64561 aaaacgtcgg gtaacgcgcg ttacacctat ctggcggcat ggggcgcagc ggataaagct
    64621 gacggtggcg ataaagccaa aaccgaacag tttatgaccc agttcctgaa aaacgttgaa
    64681 gtgttcgata ctggcggtcg tggcgcgacc accacttttg ccgagcgcgg cctgggcgat
    64741 gtgctgatca gcttcgagtc ggaagtgaac aacatccgta aacagtatga agcgcagggt
    64801 tttgaagtgg tgattccgaa aaccaacatt ctggcggaat tcccggtggc gtgggttgat
    64861 aaaaacgtgc aggccaacgg tacggaaaaa gccgccaaag cctaccttaa ctggctctac
    64921 agcccgcagg cgcaaaccat catcaccgac tattactacc gcgtgaataa cccggaagtc
    64981 atggataaac tgaaagacaa attcccgcag accgagctgt tccgcgtgga agacaaattt
    65041 ggctcctggc cggaagtgat gaaaacccac ttcaccagcg gcggcgagtt agacaagctg
    65101 ttagcggcgg ggcgtaatta atgtttgcag tttcatcaag acgcgtgctg ccgggcttta
    65161 ccttaagcct cggcaccagt ctgctgtttg tgtgcctgat tttgctgctg ccgctctccg
    65221 cgctggtgat gcagctggct cagatgagct gggcgcagta ctgggaggtg atcaccaacc
    65281 cgcaggtagt agcggcctac aaagtaacgc tgctgtcagc gtttgtggca tcgattttta
    65341 acggcgtttt tggcctgctg atggcgtgga tcttaacccg ctatcgcttt ccgggccgca
    65401 cgctgcttga tgcgctgatg gatttaccct ttgcgctgcc aacggcagtc gccggtttaa
    65461 cgctagcctc gctcttttct gtaaacggtt tttacggtga atggctggcg aagtttgata
    65521 tcaaagtcac ctatacctgg ctggggattg cggtggcgat ggcctttacc agcattccgt
    65581 ttgtggtacg taccgtgcag ccggtgctgg aagagttagg cccggaatat gaagaagcgg
    65641 cagaaacgct tggcgcaacg cgctggcaga gtttctgcaa agtggtgctg ccggaacttt
    65701 ctccggcgct ggtggcgggc gtggcgctgt cgtttacccg tagtcttggt gaatttggcg
    65761 cggtgatttt tatcgccgga aatatcgcgt ggaagacgga agtgacgtcg ctgatgattt
    65821 ttgtgcgctt acaggagttt gattacccgg cagcgagcgc gattgcttcg gtgatcctcg
    65881 cggcatctct gctgctgctg ttctcaatta acactctgca aagtcgcttt ggtcggcgtg
    65941 tggtaggtca ttaatggcgg aagttaccca attgaagcgt tatgacgcgc gcccgattaa
    66001 ctggggcaaa tggtttctga ttggcatcgg gatgctggta tcggcgttca tcctgctggt
    66061 gccgatgatt tatatcttcg tacaggcgtt cagcaaaggg ctgatgccgg ttttacagaa
    66121 tctggccgat ccggacatgc tgcacgccat ctggctgacg gtgatgatcg cgctgattgc
    66181 cgtaccggta aacctggtgt tcggcattct gctggcctgg ctggtgacgc gctttaactt
    66241 ccctggacgc cagttactgc tgacgctact ggacattccg tttgccgtat cgccggtggt
    66301 tgccggtctg gtgtatctgc tgttctacgg ctctaacggc ccgctgggcg gctggctgga
    66361 tgaacataat ctgcaaatta tgttctcctg gcccggaatg gtgctggtca ccatctttgt
    66421 gacgtgtccg tttgtggtgc gcgagctggt gccggtgatg ttaagccagg gcagccagga
    66481 agacgaagcg gcgattttgc ttggcgcgtc tggctggcag atgtttcgcc gcgtcacatt
    66541 gccgaacatt cgctgggcgc tgctttatgg cgtggtgttg accaacgccc gcgcaattgg
    66601 cgagtttggc gcggtgtcgg tggtttccgg ctcgattcgc ggcgaaaccc tgtcgctgcc
    66661 gttacagatt gaattgctgg agcaggacta caacaccgtc ggctccttta ccgctgcggc
    66721 actgttaacg ctgatggcga ttatcaccct gtttttaaaa agtatgttgc agtggcgcct
    66781 ggagaatcag gaaaaacgcg cacagcagga ggaacatcat gagcattgag attgccaata
    66841 ttaagaagtc gtttggtcgc acccaggtgc tgaacgatat ctcactggat attccttcag
    66901 gtcagatggt cgcgttgctg gggccgtccg gttccgggaa aaccacgctg ctgcgcatta
    66961 tcgccgggct ggagcatcaa accagcgggc atattcgctt ccacggcacc gacgtcagcc
    67021 gcctgcacgc gcgcgatcgc aaggtcggtt tcgtgttcca gcattacgcg ctgttccgcc
    67081 atatgacggt attcgacaat atcgcttttg gcctgacggt gctgccgcgt cgcgatcgcc
    67141 cgaatgccgc agccatcaaa gcgaaagtga caaaattgct ggagatggtg cagcttgctc
    67201 atctggcgga tcgttatccg gcacagcttt ccggcggcca gaaacagcgc gtggcgctgg
    67261 cgcgcgcgct ggctgtagaa ccgcaaattc tgctgcttga tgaaccgttt ggcgcgctgg
    67321 atgcgcaggt gcgtaaagag ctgcgtcgct ggctgcgtca actgcatgaa gagctgaaat
    67381 tcaccagcgt gttcgtgacc cacgaccagg aagaagcgac ggaagtagct gatcgtgtag
    67441 tggtgatgag ccagggcaat attgaacagg ctgatgcgcc ggagcaggta tggcgcgaac
    67501 cagcgacccg ttttgtgctc gaatttatgg gcgaagtgaa ccgcctgcag ggaaccattc
    67561 gcggcgggca gttccatgtt ggcgcacatc gctggccgct gggctacaca cctgcttatc
    67621 aggggccggt ggatctcttc ctgcgcccgt gggaagtgga tatcagccgc cgtaccagtc
    67681 tcgattcgcc gctgccggta caggtactgg aagccagccc gaaaggtcac tacacccaat
    67741 tagtggtgca gccgctgggg tggtacaacg aaccgctgac ggtcgtgatg catggcgacg
    67801 atgccccgca gcgtggcgag cgtttattcg ttggtctgca acatgcgcgg ctgtataacg
    67861 gcgacgagcg tatcgaaacc cgcgatgagg aacttgctct cgcacaaagc gcctgatagg
    67921 ttgagtgaat gttaaacgcc cggaggcgct tcccgcgatc cgggcttttt aatggcaagg
    67981 tttgtaacct gtagacctga taagacgcgc aagcgtcgca tcaggcaaca ccacgtatgg
    68041 atagagatcg tgagtacatt agaacaaaca ataggcaata cgcctctggt gaagttgcag
    68101 cgaatggggc cggataacgg cagtgaagtg tggttaaaac tggaaggcaa taacccggca
    68161 ggttcggtga aagatcgtgc ggcactttcg atgatcgtcg aggcggaaaa gcgcggggaa
    68221 attaaaccgg gtgatgtctt aatcgaagcc accagtggta acaccggcat tgcgctggca
    68281 atgattgccg cgctgaaagg ctatcgcatg aaattgctga tgcccgacaa catgagccag
    68341 gaacgccgtg cggcgatgcg tgcttatggt gcggaactga ttcttgtcac caaagagcag
    68401 ggcatggaag gtgcgcgcga tctggcgctg gagatggcga atcgtggcga aggaaagctg
    68461 ctcgatcagt tcaataatcc cgataaccct tacgcccatt acaccactac cgggccggaa
    68521 atctggcagc aaaccggcgg gcgcattacc cattttgtct ccagcatggg aacaaccggc
    68581 actatcaccg gcgtttcacg ctttatgcgc gaacaatcca aaccagtaac gattgttggc
    68641 ctgcagccgg aagaaggcag cagtattccc ggcattcgcc gctggcctgc ggaatatctg
    68701 ccggggattt tcaacgcttc tctggtggat gaggtgctgg atattcatca gcgcgatgca
    68761 gaaaacacca tgcgcgaact ggcagtgcgg gaaggaatat tctgtggcgt cagctccggc
    68821 ggcgcggttg ccggagcact gcgggtggca aaagctaacc ctggtgcggt ggtggtagcg
    68881 atcatctgcg atcgtggcga tcgctacctt tccaccgggg tgtttgggga agagcatttt
    68941 agtcaggggg cggggattta agattattgc gcaggcaccg ttttatccgt ctgcgcaatt
    69001 aaggcatgta aaaaaggctg tgcgttctca tcgctcttac cggggatctt cacaatatac
    69061 ggcttcccgg taatcgatga cgacgaagca accttatcaa taaactgctc ggcagtgtca
    69121 atgcggttac gggtattgcc gagcttcaga cgcagatgag aaaccgcttc atcgcaggta
    69181 tgttcatcac cgttgcgcac aaatatcaaa tcctttttct gtgctaatcc ctccagcatg
    69241 gcgttgatgc gggcttcttc atgaacagtt aacttcgcgt aagcggggag tgtcattagt
    69301 agtgtaatga ccagacaaat gattttcttc attgaattac cttatccttg ttttgtgata
    69361 gccgatttga ctgccagaat caggtaaagt ttcggttgga atacgatgag gatcatcggc
    69421 aaggagaaaa aattaaatga gtagtttgtt gttgtttaac gataagagta gggcgctgca
    69481 ggcggatatc gtcgccgtgc agtcgcaggt agtgtacggc agcgtaggca acagcattgc
    69541 ggtgcctgct ataaaacaga acggcttgaa tgtctttgcc gtgccgacgg tattgctgag
    69601 caatacgccg cattatgaca ctttctacgg tggtgcgatt ccggacgaat ggtttagcgg
    69661 ctatttgcgt gcgcttcagg agcgtgatgc gctgcgccaa cttcgtgctg taaccacggg
    69721 ctatatggga acggcatcgc aaatcaaaat ccttgccgag tggctgactg cgctacgcaa
    69781 agaccatcct gacctattga tcatggtcga tccggtgatt ggcgatattg atagcggaat
    69841 ttatgtcaaa cctgaccttc ccgaggcgta tcgacaatat ttactgccgc tggcgcaagg
    69901 aattaccccc aatatctttg agctggaaat cctgaccggt aaaaattgcc gcgatctcga
    69961 cagtgccatt gctgccgcaa aaagtctgct ttcagacaca ttaaaatggg tggtgattac
    70021 cagcgcctcc ggtaatgaag aaaatcagga gatgcaggtg gtggtggtca gtgccgacag
    70081 cgtgaatgtc atttcccatt cacgggtaaa aaccgacctg aaagggactg gcgacctgtt
    70141 ttgtgctcag cttatcagtg gtttgctgaa agggaaggcg ttaaacgatg cggtgcaccg
    70201 tgcggggttg cgcgtactgg aagtgatgcg ctacacccag cagcatgaga gcgatgaatt
    70261 gattttgccg ccgctggcgg aagcataaaa aatggcgccg atgg
//
LOCUS       DAQSWQ010000008        65646 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.008, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000008 DAQSWQ010000000
VERSION     DAQSWQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.008"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..616
                     /locus_tag="R8G18_000539"
     CDS             <1..616
                     /locus_tag="R8G18_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9794334.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
     gene            complement(613..2091)
                     /gene="scpC"
                     /locus_tag="R8G18_000540"
     CDS             complement(613..2091)
                     /gene="scpC"
                     /locus_tag="R8G18_000540"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HEE9794335.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2115..2900)
                     /gene="scpB"
                     /locus_tag="R8G18_000541"
     CDS             complement(2115..2900)
                     /gene="scpB"
                     /locus_tag="R8G18_000541"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HEE9794336.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(2911..3906)
                     /gene="meaB"
                     /locus_tag="R8G18_000542"
     CDS             complement(2911..3906)
                     /gene="meaB"
                     /locus_tag="R8G18_000542"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEE9794337.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(3899..6043)
                     /gene="scpA"
                     /locus_tag="R8G18_000543"
     CDS             complement(3899..6043)
                     /gene="scpA"
                     /locus_tag="R8G18_000543"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEE9794338.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(6246..7139)
                     /gene="argP"
                     /locus_tag="R8G18_000544"
     CDS             complement(6246..7139)
                     /gene="argP"
                     /locus_tag="R8G18_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HEE9794339.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7569..8228
                     /gene="rpiA"
                     /locus_tag="R8G18_000545"
     CDS             7569..8228
                     /gene="rpiA"
                     /locus_tag="R8G18_000545"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEE9794340.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8484..9716
                     /gene="serA"
                     /locus_tag="R8G18_000546"
     CDS             8484..9716
                     /gene="serA"
                     /locus_tag="R8G18_000546"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEE9794341.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            9945..10004
                     /gene="ibsC"
                     /locus_tag="R8G18_000547"
     CDS             9945..10004
                     /gene="ibsC"
                     /locus_tag="R8G18_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HEE9794342.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10336..10395
                     /locus_tag="R8G18_000548"
     CDS             10336..10395
                     /locus_tag="R8G18_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HEE9794343.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10496..11098)
                     /locus_tag="R8G18_000549"
     CDS             complement(10496..11098)
                     /locus_tag="R8G18_000549"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEE9794344.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11119..11302)
                     /gene="ssrS"
                     /locus_tag="R8G18_000550"
     ncRNA           complement(11119..11302)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="R8G18_000550"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11344..11673)
                     /gene="zapA"
                     /locus_tag="R8G18_000551"
     CDS             complement(11344..11673)
                     /gene="zapA"
                     /locus_tag="R8G18_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEE9794345.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            11841..12419
                     /gene="ygfB"
                     /locus_tag="R8G18_000552"
     CDS             11841..12419
                     /gene="ygfB"
                     /locus_tag="R8G18_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HEE9794346.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12445..13770
                     /gene="pepP"
                     /locus_tag="R8G18_000553"
     CDS             12445..13770
                     /gene="pepP"
                     /locus_tag="R8G18_000553"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEE9794347.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13767..14945
                     /gene="ubiH"
                     /locus_tag="R8G18_000554"
                     /gene_synonym="visB"
     CDS             13767..14945
                     /gene="ubiH"
                     /locus_tag="R8G18_000554"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEE9794348.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            14968..16170
                     /gene="ubiI"
                     /locus_tag="R8G18_000555"
                     /gene_synonym="visC"
     CDS             14968..16170
                     /gene="ubiI"
                     /locus_tag="R8G18_000555"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HEE9794349.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16244..16327)
                     /locus_tag="R8G18_000556"
     CDS             complement(16244..16327)
                     /locus_tag="R8G18_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794350.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16617..17711
                     /gene="gcvT"
                     /locus_tag="R8G18_000557"
     CDS             16617..17711
                     /gene="gcvT"
                     /locus_tag="R8G18_000557"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEE9794351.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            17735..18124
                     /gene="gcvH"
                     /locus_tag="R8G18_000558"
     CDS             17735..18124
                     /gene="gcvH"
                     /locus_tag="R8G18_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEE9794352.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18243..21116
                     /gene="gcvP"
                     /locus_tag="R8G18_000559"
     CDS             18243..21116
                     /gene="gcvP"
                     /locus_tag="R8G18_000559"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEE9794353.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21382..22125
                     /locus_tag="R8G18_000560"
     CDS             21382..22125
                     /locus_tag="R8G18_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEE9794354.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(22182..23615)
                     /gene="bglA"
                     /locus_tag="R8G18_000561"
     CDS             complement(22182..23615)
                     /gene="bglA"
                     /locus_tag="R8G18_000561"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HEE9794355.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            23660..23971
                     /locus_tag="R8G18_000562"
     CDS             23660..23971
                     /locus_tag="R8G18_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEE9794356.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24135..24794
                     /locus_tag="R8G18_000563"
     CDS             24135..24794
                     /locus_tag="R8G18_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEE9794357.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(24990..25970)
                     /gene="ygfZ"
                     /locus_tag="R8G18_000564"
     CDS             complement(24990..25970)
                     /gene="ygfZ"
                     /locus_tag="R8G18_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HEE9794358.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26213..26479
                     /gene="sdhE"
                     /locus_tag="R8G18_000565"
     CDS             26213..26479
                     /gene="sdhE"
                     /locus_tag="R8G18_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HEE9794359.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26460..26867
                     /locus_tag="R8G18_000566"
     CDS             26460..26867
                     /locus_tag="R8G18_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HEE9794360.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(26907..27428)
                     /gene="fldB"
                     /locus_tag="R8G18_000567"
     CDS             complement(26907..27428)
                     /gene="fldB"
                     /locus_tag="R8G18_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HEE9794361.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27540..28436
                     /gene="xerD"
                     /locus_tag="R8G18_000568"
     CDS             27540..28436
                     /gene="xerD"
                     /locus_tag="R8G18_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEE9794362.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28461..29171
                     /gene="dsbC"
                     /locus_tag="R8G18_000569"
     CDS             28461..29171
                     /gene="dsbC"
                     /locus_tag="R8G18_000569"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HEE9794363.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            29177..30910
                     /gene="recJ"
                     /locus_tag="R8G18_000570"
     CDS             29177..30910
                     /gene="recJ"
                     /locus_tag="R8G18_000570"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEE9794364.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31001..32099
                     /gene="prfB"
                     /locus_tag="R8G18_000571"
     CDS             join(31001..31075,31077..32099)
                     /gene="prfB"
                     /locus_tag="R8G18_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEE9794365.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32109..33626
                     /gene="lysS"
                     /locus_tag="R8G18_000572"
     CDS             32109..33626
                     /gene="lysS"
                     /locus_tag="R8G18_000572"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEE9794366.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33669..34217)
                     /gene="idi"
                     /locus_tag="R8G18_000573"
     CDS             complement(33669..34217)
                     /gene="idi"
                     /locus_tag="R8G18_000573"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEE9794367.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34272..34343
                     /locus_tag="R8G18_000574"
     CDS             34272..34343
                     /locus_tag="R8G18_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794368.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34340..34465)
                     /locus_tag="R8G18_000575"
     CDS             complement(34340..34465)
                     /locus_tag="R8G18_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794369.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34467..35545)
                     /locus_tag="R8G18_000576"
                     /pseudo
     CDS             complement(34467..35545)
                     /locus_tag="R8G18_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35981..37900
                     /gene="ygfT"
                     /locus_tag="R8G18_000577"
     CDS             35981..37900
                     /gene="ygfT"
                     /locus_tag="R8G18_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HEE9794370.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            37900..38388
                     /locus_tag="R8G18_000578"
     CDS             37900..38388
                     /locus_tag="R8G18_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEE9794371.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(38457..38807)
                     /gene="ghxQ"
                     /locus_tag="R8G18_000579"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(38457..38807)
                     /gene="ghxQ"
                     /locus_tag="R8G18_000579"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(38843..39157)
                     /locus_tag="R8G18_000580"
     CDS             complement(38843..39157)
                     /locus_tag="R8G18_000580"
                     /inference="COORDINATES: protein motif:HMM:NF014081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEE9794372.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(39090..42029)
                     /locus_tag="R8G18_000581"
     CDS             complement(39090..42029)
                     /locus_tag="R8G18_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HEE9794373.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(42026..42805)
                     /locus_tag="R8G18_000582"
     CDS             complement(42026..42805)
                     /locus_tag="R8G18_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HEE9794374.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(42856..44184)
                     /gene="ssnA"
                     /locus_tag="R8G18_000583"
     CDS             complement(42856..44184)
                     /gene="ssnA"
                     /locus_tag="R8G18_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HEE9794375.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(44187..47285)
                     /gene="ygfK"
                     /locus_tag="R8G18_000584"
     CDS             complement(44187..47285)
                     /gene="ygfK"
                     /locus_tag="R8G18_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HEE9794376.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(47607..48185)
                     /gene="mocA"
                     /locus_tag="R8G18_000585"
     CDS             complement(47607..48185)
                     /gene="mocA"
                     /locus_tag="R8G18_000585"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HEE9794377.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            48288..49058
                     /gene="yqeC"
                     /locus_tag="R8G18_000586"
     CDS             48288..49058
                     /gene="yqeC"
                     /locus_tag="R8G18_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HEE9794378.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            49106..50731
                     /locus_tag="R8G18_000587"
     CDS             49106..50731
                     /locus_tag="R8G18_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HEE9794379.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(50772..51704)
                     /gene="arcC"
                     /locus_tag="R8G18_000588"
     CDS             complement(50772..51704)
                     /gene="arcC"
                     /locus_tag="R8G18_000588"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEE9794380.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(51752..53137)
                     /gene="hyuA"
                     /locus_tag="R8G18_000589"
     CDS             complement(51752..53137)
                     /gene="hyuA"
                     /locus_tag="R8G18_000589"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HEE9794381.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(53190..54401)
                     /locus_tag="R8G18_000590"
     CDS             complement(53190..54401)
                     /locus_tag="R8G18_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HEE9794382.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(54459..55655)
                     /gene="dpaL"
                     /locus_tag="R8G18_000591"
     CDS             complement(54459..55655)
                     /gene="dpaL"
                     /locus_tag="R8G18_000591"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEE9794383.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(55713..56900)
                     /gene="ygeW"
                     /locus_tag="R8G18_000592"
     CDS             complement(55713..56900)
                     /gene="ygeW"
                     /locus_tag="R8G18_000592"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HEE9794384.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            57379..59157
                     /locus_tag="R8G18_000593"
     CDS             57379..59157
                     /locus_tag="R8G18_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HEE9794385.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(59197..59676)
                     /gene="xdhC"
                     /locus_tag="R8G18_000594"
     CDS             complement(59197..59676)
                     /gene="xdhC"
                     /locus_tag="R8G18_000594"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HEE9794386.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(59673..60551)
                     /gene="xdhB"
                     /locus_tag="R8G18_000595"
     CDS             complement(59673..60551)
                     /gene="xdhB"
                     /locus_tag="R8G18_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HEE9794387.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(60562..62859)
                     /gene="xdhA"
                     /locus_tag="R8G18_000596"
     CDS             complement(60562..62859)
                     /gene="xdhA"
                     /locus_tag="R8G18_000596"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HEE9794388.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            63274..64029
                     /locus_tag="R8G18_000597"
     CDS             63274..64029
                     /locus_tag="R8G18_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEE9794389.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            64108..64177
                     /locus_tag="R8G18_000598"
     tRNA            64108..64177
                     /locus_tag="R8G18_000598"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64140..64142,aa:Gly,seq:ccc)
     gene            64370..>65646
                     /locus_tag="R8G18_000599"
     CDS             64370..>65646
                     /locus_tag="R8G18_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794390.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT        15771 a        16327 c        17396 g        16152 t
ORIGIN      
        1 aattgtgatt ggcatagata atacttatcc gacaattatt ttcgatcagt tgattagtct
       61 cggagataag tatgaaggcg tgaccgccca gccagttgag ttcagcgaaa acggcgttat
      121 cgataatctt tttgaccgtc agttagattt tattatctcc ccacagcatg tatctgcacg
      181 agttcaggaa ctggaaaacc tgactatcag tgaattacca ccgctgcgat tgggctttct
      241 cgtttctcgc cgttatgaag agcgtcaaga gcaagagctg cttcaggagc tgccctggct
      301 acaaatgcgc tttcaaaacc gggctaactt cgaagcaatg attgatgcga atatgcgacc
      361 ttgtgggatc aatcccacca ttatttatcg tccctacagt tttatggcca aaatcagcgc
      421 cgtggagcgc ggacattttc tgacggtgat cccgcatttt gcctggcgac tggtgaatcc
      481 ggcaacgctg aaatattttg atgcgccgca caggccgatg tatatgcagg aatatcttta
      541 ttcaatcaga aatcatcgtt ataccgccac gatgcttcag catattgctg aagatcgtga
      601 cgggacaaac cattaaccca gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa
      661 gacgtggcta agatcgtggt gaatatgtcc gccgggcgca ttttccagat agcgatgcag
      721 ataatcccga tacataggat gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg
      781 ggaaagaccg cgtagatcgg cgatcccttg ttcggtgatg atcactttga cgctgtgttc
      841 gctgtgatca acatggctgc acattggcac gacggttgag atcttgcctt ctttagcaat
      901 cgacggagcc ataaagatcg acagatacgc gttgcgttca aaatcaccgc tgccgccgat
      961 gccgttcatc agatcgaccc cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa
     1021 ctccaggccg acgttcagag cgatgacgcc cagacgacgg atgatttccg ggttattgga
     1081 aatctcctgc ggacgcaaca caatgcggct ggcaaagtaa tccatattgt cgtaaatctt
     1141 gcgcagggaa tcggccgaga ttgtcaggct ggaggcgctg gccccgctga ttttgccggt
     1201 ttccagtaaa tgcaccaccg attcctgtag cacttccgaa tacatcatga acggaggaat
     1261 ttccgggttt tcccccagac gcgccattac cgcattattg atattgccca cgccactttg
     1321 cagcggcaga aattccggcg gaatacgccc atgcgccatt tcctgcaata agaacgtgac
     1381 cacgttatcg gcaatctgct ggcacatggg attttgctta tccagcatat taccggcgtc
     1441 gggcaagttg gtttccacga cggcgacaat ctttttcgga tcgatttgca catagcgggt
     1501 accgacgcga tccattgcat ggaagatcga cacgctattg cgccgcggtg gtgcgccagg
     1561 aatcacaata tccgccagtt ctgcaacgcg cggatcgtga tagtgattaa gttcaatgat
     1621 cactttcttc gcccgcagca gccatgtcgg cgcattaccg atcccgctgg ttaaccagac
     1681 tcgaccatcc ggtgccagtg ccgatgcttc gatgacggca acatcaatgt cgccgaagaa
     1741 accgtaattg accatttgcg ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc
     1801 ctgattgatc tttttacgta aaccggacga tgtttgatat ggcgcacgcc aggaaacagc
     1861 atcggcgtca gaaagtacat cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg
     1921 aatttgatac ggctttttgg cctcatgctg ttcgttagct ctgcgggcaa tcgcggtcgg
     1981 taaggctttc ggcgaacccg ctggggtaaa gccgctaaat gccaccatgt cgttatgctg
     2041 gataatttct gccgcttcat cggcggtcat ccttgtccac tgagtttcca tttttactcc
     2101 ttcgttcgca gggattaatg accaacgaaa ttaggtttac gtttttcgag gaaagcgttc
     2161 atcccttcct ggtaatcttc gctgtcataa accgcgcggc gcatcccctg aatacgttca
     2221 aattcatcgg agttcatggt gtgcgcttcg cccagtacac gcagctcttc tttgataaca
     2281 gcaatggcta acggcgcttt ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc
     2341 agttcttcca cttccacaac atggttgagg atgccgacag ccaacgcgcg ctgggcggtg
     2401 attggcgaag cggtaaaaat cagctctttg acaatgtgga agcccgcgtc gcgggtcagg
     2461 ttgtgaatgc cgaccaggtt atacgggacg ccgaggttta caggcatcat tgagaaggtt
     2521 gaggtactgg cggcgatgat cagatcggaa ctcatgatca tttcaaatgc gccaccccaa
     2581 acactacctt ccaccatcga aatgatcggt ttcgggaatt tttggatcat gcgggtgatt
     2641 tgacgcaatg gatcatcata ggagagcgga tcgcgaccgc cagacggcag ttcgtgaata
     2701 tcgtgacctg cggagaagac tttggatcca cttggcgccc gcaaaatgat acagcgaatt
     2761 tccggccggt tgagatcgct taacgcctgc ataagatcat caataaagac tttacttaag
     2821 gcattaagtt ttcgtccata gttaaactca atgaccgcca ctttgttgat agtggcaacg
     2881 ttcacatact gataagacat aaaaattcct ttaatcaaaa tattgcgtct ggataaattc
     2941 actgagctgc cgcaggccgg tgcgcggtga gagcgtattg tttttgaccg ctaaaagcgt
     3001 ctggcggtaa tagcgatcga aatcttcatt cgcgaacagg tgattcagta cttcttcttc
     3061 ggtctgctta cgcagccatt ccaccgattg ttgttgccgc acttgttgta aacgaccact
     3121 ggcagttagc gcggttttga agtcgatgat ggcgtgccag atctcatcga ttccacgttt
     3181 ttccagtgcg ctacaagtca gaacccgtgg ctgccattcg tcgtatttac gtcgcagaat
     3241 atgcagggca ctctcgtaca tatgccgggc aatggcgaca ttggtatggt tatcgccatc
     3301 gtctttgttg ataacgagca gatcagccac ttccatcagc ccttttttaa tgccctgcag
     3361 atcatcgccg ccaccggcaa tttgcaacga gataaaacag tccaccatgc gggcgacttc
     3421 cgtttccgac tgcccgacgc caaccgtttc gacaatcact acgtcataac ccgccgcttc
     3481 gcataacagc attaattccc gtgctcgctg actggcaccg cccagatgac cggaggaggg
     3541 taccgggcga ataaacgccg cctcggcacg cgccaggtca ttcatgcggg ttttatcccc
     3601 gagaatgcta ccgccagtga ccgggctgct gggatcgacc gcaataacag caacctttaa
     3661 tccctcacga atcaacaaca tgccaaaggc ttcaagaaag gtacttttcc ccgcgccggg
     3721 tgtgccggta acgcccagtc gcagggcgtt accacagaac ggcataatgg cgtcaagcag
     3781 ctgtgtactt agcgcctgat gacgtgggtg acggctttcc accagcgtca tggcctgggc
     3841 gagcgtggca cgctcaccct gacgtaagcg gcgaatactt tctgccagcg tggcttcatt
     3901 aatcatgatg ctggcttatc agattcagta cgtcgcgcac actgtcgagc ataggtgtac
     3961 ctggaccata aatcgccgcc acgccgcgct cttgcaggaa ggcgtaatcc tgcggcggaa
     4021 tgacgccacc cgcgaccacg cagatatctt cgcgtcccca ttttttcagc gcttcgacca
     4081 gttccgggat cagcgtttta tgatcggcag ccagtgagga tgcgcccact acgtgaacgt
     4141 cgttttctac ggccaggcgg gcgatctctt caggtgtaga gaacatcggg cttaaatcta
     4201 cgtcgaaacc gagatcggaa taggcgctgg cgatcacttt cgcgccgcga tcgtgtccat
     4261 cctggcccat cttagcgatc agaatgcgcg ggcgacgacc attgtcggca aggaactgct
     4321 ccgtttgcgc aacaatggca tcgaactcgg aggccgattt ctcagactga tgatagcttt
     4381 gcgcaatcac gccggtaaca cactggcttg gcaccagata acggtcgaaa gcgacttcca
     4441 gcgcatcgga aatttcaccc agggtggcgc gaacgcgagc ggcattaaca gcggcagcca
     4501 gcaggttttc gttatgctgt gcggcgtgag tcagggcgtt caacgcggcg gttacggcgg
     4561 catcatcacg ggtggcgcga atgcgttcca gcgaagcaat ttgctcgtta cgcaccatca
     4621 cgttgtcgat ctcaagtaca tcggtttcgt cttcgtgatc cagtttgtac ttgttgacac
     4681 caacgatgac acgcttgcct tggtcgatca gcgactgttc gcgcgctgag gcctcttcga
     4741 tcattcgttt tggcagacct gcttcgatcg ctttcgccat gccaccggct tcgtcgatct
     4801 gttggataat agctctggct tgtttgacga tttgatcggt cagcgactca atgtaatagg
     4861 atccggccag tggatcgacg gtgcggcaga gttctgattc ttcctggatg atgatctggg
     4921 tgttgcgggc aatgcgtgct gagaaatcgg taggcaaacc aagcgcttcg tcaaaggcgt
     4981 tggtatgcag tgactgagta ccgcccagcg tcgcagccag tgcttcaatg gtggtgcgga
     5041 taacgttgtt atacggatcc tgttcagtca ggctccagcc tgaggtctgg cagtgggtac
     5101 gcagcgccag tgatttcgga tcctgtgcgc caaatccact gaccgcttcg ctccataaat
     5161 aacgtgccgc acgcaacatg gcgacgttca taaacagatc catgccgata ccgaagaaga
     5221 acgacaggcg aggagcgaag tcatcaattt tcagtccggc agagattgct gctttgatgt
     5281 actcaatccc atcggcgagc gtaaatgcta cctgctgcac gcagttggca cccgcttccc
     5341 ccatgtggta accgctgata ctgatggtat taaatcgcgg catgttgccg gaacaccagg
     5401 cgatgatgtc ggcgataatg cgcattgacg gttttggtgg gtaaatatag gtgttgcggc
     5461 agaggtactc tttgagaata tcgttttgaa tggtgccggt cagtttatca ggtgtaacac
     5521 cttgctcttc tgcggcgacg atataaaacg ccagtactgg tagcactgcg ccattcatgg
     5581 tcatcgaaac cgacatttta tccagcggga tctggtcgaa caggactttc atatcttcca
     5641 cggtgtcgat agcgacgccc gctttgccga cgtcacccgc cacgcgtggg ttatcggagt
     5701 cgtagccacg gtgggtggca aggtcaaacg caacggaaag acctttttgc ccggcggcca
     5761 ggttacggcg ataaaaagcg ttggactctt ttgctgttga aaaaccagca tactgacgga
     5821 tggtccacgg ttgggcggta tacatagtgg cacgcgggcc acgaacgtag ggcggcaaac
     5881 caggaagggt acctgtcacc tccagattat cgagatcggc ttcggtatac agctgcttga
     5941 tggcgatccc ttccgcggtt tgctgaacca gcgagtcgac agttttctcc cgacggctca
     6001 attccttgtt ggcaagctgt tgccactcct gctcgttaga cattatttgc tccgtaaaac
     6061 gcaatcaaaa tgagggtatt tgcgcttacg gtgagctttt tacgtgggtt tgtcgccagc
     6121 aaaaaaatgc attcagacgt cgttttttgc cgcctgagat ttgtctattt tgtgatgtga
     6181 ttcaaggaat acaaaaagaa gtgaggataa cgcctgatat gtgctatcag gcattatttg
     6241 atggattaat cctgacgaag gactttgtga ccataatcga gcaacgcatc agtgacttta
     6301 cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga gcatccggcg ttgaaatagc
     6361 cctggcgtta agtcaatcag ttcaccgctg gctagttctt tctcgatttg caggtgcggg
     6421 atcatacagc aggtggtgcc ctggcgagca agttgcacga acgcttctga agaattaacg
     6481 atatggcagg gcacgctgcc tggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc
     6541 atatcgtcaa gatggtcaaa ggcgaccaca ggtgctttca gtaatgccga acgcgttacg
     6601 ccgttaggga aatatttttc ggcaaagggt tttgagctga cgaacagata gtcgagcgca
     6661 ccaagtttat cgacaagaca actcggcagc gcctgatgtt gaatactcac cgcgccgacc
     6721 acttcgccgc ggcgcagacg ttcctgagta cgggtttcat cttctacctg caagttgagg
     6781 cggataggcg aatcagccaa cacaggagcc agtgccggaa gcaaccacgt cgccagactg
     6841 tcggcgttga ccgccagtga aagcagcagc ggagtcgaac cggtttgttc atcgcccagc
     6901 cactcttctt ccagcaactc cacctggcgc agcagtgcca gcagtttttg cccttgttcc
     6961 gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc cgaacatatt ttccagttgc
     7021 ttaatgcgct gtgagacggc tgattgtgta atgcacagct tttgtgccgc gcgctcaaat
     7081 cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt
     7141 gctgttgtct gctcctggta aatgcttttt cctgcactat gacacaattt tgtgtcaggt
     7201 tgcatatacc gaagccgtcg gcgggtaaca tttgtatccg tggttaaacg cgcagcttaa
     7261 aagatcaggg atttgcgcta atgcatttcg ctcagcgagt tcgtgccctg gttgtgttga
     7321 atggggtggc gttattgcct caatttgcct gtaaacaggg gcttgcgaac ggggggaact
     7381 ggtgcgcctg ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg
     7441 gcgaaagggg atgcccgcca ttgcgcgata ttttatggat gagttaacca cgcggcttgc
     7501 caacggggtc tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg
     7561 aaacgatcat gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg
     7621 ttcagcccgg caccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg
     7681 cgctcggtac aatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg
     7741 aaaaactgaa aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca
     7801 tctacgttga tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg
     7861 cggcgctgac ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgctg
     7921 acgcttccaa gcaggttgat attctgggca aattcccgct gccagtagaa gttatcccga
     7981 tggcacgtag tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc
     8041 agggcgtggt gaccgataac ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg
     8101 acccgatagc gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt
     8161 ttgctaaccg tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg
     8221 tgaaatgatc tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg
     8281 acatgtgtca cgtttttact tggctattgt cgattgctct aaataaatcc tctaaaccag
     8341 catattcaac caagaaatac ctttgcgtga tatttcctca acatcgcgac gcaaacgttc
     8401 atattgccgc aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca
     8461 tttcaaaaga caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt
     8521 ttctgctggt agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca
     8581 ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg
     8641 atgcccactt catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg
     8701 cagaaaaact ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg
     8761 cggcggcaaa gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg
     8821 cggagctggt gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta
     8881 aagcgcaccg tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa
     8941 agctgggtat catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc
     9001 tgggaatgta tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc
     9061 aggtacagca tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag
     9121 agaatccgtc caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aagcccggct
     9181 cgctgctgat taatgcctcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc
     9241 tggcgagcaa acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca
     9301 atagcgatcc atttacctct ccactgtgtg aattcgacaa cgtccttctg acgccacaca
     9361 ttggcggttc gactcaggaa gcgcaggaga atatcggcct ggaagttgcg ggtaaattga
     9421 tcaagtattc tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc
     9481 cactgcatgg tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg
     9541 cgctgaacaa aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt
     9601 ccgcccagat gggttatgta gttattgata ttgaagctga cgaagacgtt gccgaaaaag
     9661 cgctgcaggc aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc
     9721 ccttctctga aaattaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac
     9781 accgttgccg cgttgcgcat tctactttgc gagtctgctc cagaaaataa atcaaactca
     9841 gatcaatgcg ttgtgtttat ttaatgggga tttacgatgg cgggacagca tggggctgta
     9901 acggtaaagc cctcaccgaa gcgagggctt aaaggagaag ggttatgatg cgacttgtca
     9961 tcatactgat tgtactgtta ctgataagtt tcggagctta ttaacagtca gtctcagggg
    10021 aggagtcatc ctctcttacc cttactcact aaattaggtc aaagaatcaa cgatgtcaat
    10081 cagggcgatg cggggtgcat cgccctgaac cgaacttttt gatagccact tcatcgccca
    10141 cgtttactgc cgtaatagat cctttttccc aaaaattatc tctgttttgc atgggttttt
    10201 cgagcacgct cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga
    10261 gcttacgata gctgggcagc atggggctgt aacggtaaag ccctcaccga agcgagggct
    10321 tgaaggagaa gggttatgat gcgacttgcc atcatactga ttgtactgtt actcataagt
    10381 ttcccagctt attaacagtc agtcacaggg gaggagcaat cctcccttac ccttactcac
    10441 taaattaggt taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc
    10501 actcccagac tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg
    10561 ggagtttttc caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct
    10621 gccagttttg taaggtccga tcataaaaac caccgcccat tcccaggcgc tgaccgtact
    10681 catcaaaggc gaccagcggt gtgatcagca cgtctaatcg ggaaaggggt agcacgtcac
    10741 gcacatccag ttttggctca tggatcttca acctgttcat caccagttcg ctttgcggat
    10801 ggtaattcag gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc
    10861 gcttaccggc gcgccagagt tgttctatta gcggctgggt gtcgagttca ccatcaaaag
    10921 agaggaatac agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag
    10981 cggcttgttg acccatttcc tgctgttgtt ccggcgttaa cgcacgacga cgttgccgaa
    11041 tcattttgcg gatttcttgt cgggataatg tcagtgggag ttctggtagt tgcgtcatgg
    11101 ctggtgccag ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc
    11161 ttggttcaag gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacggac cgagcatgct
    11221 caccaaccgc ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg
    11281 cttgcgaaca tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa
    11341 gtgtcattca aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg
    11401 ttctatggtc tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt
    11461 cttcgctttt tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg
    11521 ttcagtattt gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc
    11581 cgctgcctga ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc
    11641 aaaaatttgg atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct
    11701 gccttcgtct ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa
    11761 gagacaagcc cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct
    11821 ccctttgacg acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga
    11881 accagtatct gaaccaacaa gggacggggc tgacccctgc cgagatgcat ggtttaatca
    11941 gtgggatgat atgtggcggt aacgatgaca gctcatggct gccgctactt cacgacctga
    12001 cgaacgaagg catggctttc ggtcatgagc tggcacaggc actgcgtaaa atgcactctg
    12061 ccaccagcga tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg
    12121 atgatgtcag cgttttcgat cgggctgatg cattggcagg ttgggtcaat cacttcctgc
    12181 ttggtcttgg cgttacgcaa ccgaagctgg ataaagtgac cggcgaaacc ggtgaagcta
    12241 tcgacgatct gcgtaacatt gcgcaactgg gttacgacga agacgaagat caggaagagc
    12301 ttgaaatgtc gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg
    12361 acacctttac tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa
    12421 aacaaaaaac gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc
    12481 cgtcaggccc tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca
    12541 gaagtaacac gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac
    12601 ttcaccggct ttaacgaatc ggaagcggtg ctggtgctga ttaaaagcga tgacactcat
    12661 aaccacagcg ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt
    12721 cgcttaggcc aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc
    12781 gaaatcaatc agcaacttta tcaactactt aacggcctgg atgtggttta ccatgcccag
    12841 ggcgaatatg catatgctga tgaaatcatg aacagtgcgc tggaaaaact gcgtaaaggt
    12901 tcgcggcaaa atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa
    12961 atgcgcctgt tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc
    13021 gccatggcac atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg
    13081 gaaggcgaaa ttcaccacga atttaaccgc cacggtgcgc gctatccgtc ctataacacc
    13141 attgtcggca gcggtgaaaa cggctgcatt ctgcactaca ccgaaaacga gagtgaactg
    13201 cgcgacggtg acctggtgtt gattgacgcg ggttgtgaat acaaaggtta cgcgggcgat
    13261 attacccgca ccttcccggt caacggcaaa ttcacccagg cccagcgtga aatctacgac
    13321 attgtgctgg agtctctcga aaccagcctg cgcctgtatc gtccgggaac ttccattctg
    13381 gaagtcactg gtgaagtggt gcgcatcatg gttagcggcc tggtaaaact cggcatcctg
    13441 aaaggtgatg ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc
    13501 cttagccact ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg
    13561 cgcattctgg aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat
    13621 gcggatgtgc cagaacaata tcgcggtatc ggcattcgta ttgaagacga cattgtgatt
    13681 accgaaaccg gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa
    13741 gcgttgatgg cagctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg
    13801 gcgcgacgct ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga
    13861 ttgaagcgac cgcgccagag tcacatgctc atccgggctt tgatggacga gctattgcgc
    13921 tggctgcggg gacctgtcag caactggcgc gcatcggcgt ctggcaatct ctggcggatt
    13981 acgcaaccgc catcactacc gtgcatgtca gcgatcgtgg tcacgctgga tttgtcaccc
    14041 tcgccgcaga agattaccaa ctggcggcgc tgggacaggt tgtcgaattg cacaatgtcg
    14101 ggcaacggct gtttgcattg ctgcgtaaag cacctggcgt aacgctgcat tgccctgatc
    14161 gcgtggctaa cgttgcccgt actcagagtc acgttgaagt gacgctggag agtggcgaga
    14221 cgctgacggg ccgcgtgctg gtagcagctg atggcaccca ttcagcgtta gccaccgcgt
    14281 gcggcgttga ctggcagcag gagccttacg aacaactggc cgtgattgcc aacgttgcga
    14341 cttccgttgc gcacgaaggc cgcgcttttg aacgcttcac gcaacatggc ccgctggcga
    14401 tgttgccgat gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg
    14461 aagaggtgct gtcgtggagc gacgagaagt tttgccgtga actccagtcg gcctttggct
    14521 ggcgacttgg aaaaattacc cacgccggta aacgcagtgc ttatccgctg gcgttaaccc
    14581 gcgccgccag agctattacc catcgtaccg tgctggtggg caatgcggcg caaactctgc
    14641 acccgattgc cgggcaaggg tttaacctcg gtatgcgaga tgtcatgagc cttgcggaaa
    14701 ccctgactca ggcgcatgag cgcggagaag acatcggtga ttacggcata ttgtgccgct
    14761 atcagcagcg tcgacagagc gatcgcgaag caaccatcgg cgtcacggac agcctggtac
    14821 atctttttgc caaccgttgg gcaccgctgg ttgtcgggcg caacatcgga ctgatgacga
    14881 tggaattatt caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc
    14941 gttgaaggcg gttttaagga ataaacaatg caaagtgttg atgtagccat tgttggtggc
    15001 ggcatggtgg ggctggcggt tgcctgcggc ttacagggga gcggcttacg cgttgccgta
    15061 ctggagcagc gcgtaccgga acctctggcg gcggatgcac caccacaact gcgcgtttcg
    15121 gctatcaatg ccgccagcga aaaattactc acccgtcttg gcgtctggca ggacattctc
    15181 tctcgtaggg ccagttgtta tcacggtatg gaagtgtggg acaaagacag ttttggtcac
    15241 atttcgtttg acgatcaaag catgggctat agccatcttg ggcatatcgt tgaaaactca
    15301 gtgattcact acgcgctgtg gaacaaagcg cagcagtcgt cagatatcac tctgttggcc
    15361 cccgcagaat tacagcaggt cgcctgggga gaaaatgaaa ctttcctgac gctgaaagat
    15421 ggcagcatgt taacggcgcg tctggtgatt ggcgcggacg gtgctaattc ctggttgcgc
    15481 aacaaagcag atattccgct gactttctgg gattatcagc atcacgcgct ggtagcgacc
    15541 attcgcacgg aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg cgaaggcatt
    15601 ctggcctttt taccgcttag cgatccgcat ctttgctcaa ttgtctggtc actgtcgcca
    15661 gaggaagcgc agcggatgca gcaggcaagt gaagatgaat ttaatcgcgc gttaaatatc
    15721 gcttttgata atcgcctggg cttatgcaag gttgagagcg cgcgtcaggt gttcccactg
    15781 acggggcgtt atgcgcgcca gtttgccgcg caccgtctgg tgctggtggg cgacgccgcg
    15841 cataccattc acccgctggc ggggcaggga gtaaatcttg gctttatgga tgctgcagag
    15901 ctgattgctg aactgaaacg gttgcatcgt caggggaaag acatcgggca gtacatttac
    15961 ctgcgccggt atgagcgtag ccgcaagcac agtgcggcgc tgatgctggc tggtatgcag
    16021 ggattccgcg atctgttttc cggtaccaat ccggcgaaaa aactgctgcg tgatattggt
    16081 ttgaaactgg ccgacacgct tcctggcgtt aaaccacaac ttatccgcca ggcaatggga
    16141 ttaaacgatt tgcctgaatg gctgcgttaa aaatttctct tctgctgttt atttgatacc
    16201 catcacactt tcatctcccg gttttttcgc cgggagattt tcctcatttg aaataaacta
    16261 atttcacctc cgttttcgca ttatattttc taatgtcatt attttttgat ttagtgtttt
    16321 ttgacatttt tttagctctt aatattgcct tattcaaatt agctttctaa tggcatcatc
    16381 tttatataga aactggtgtg ttttttggtt ttttattctg tcgcgatttt ggcgtttttt
    16441 aaccataagc taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta
    16501 agttcaggca aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttc
    16561 gtggtgataa tttcaccatg aaaagttgtc agccccgctt attcaatgag gacaagatgg
    16621 cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc
    16681 acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta
    16741 ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt cgatctccgc ggcagccgca
    16801 cccgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca
    16861 aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgac ctcatcgtct
    16921 actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc
    16981 tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt cgtgatgacc
    17041 tttccatgat tgccgtgcaa gggccgaatg cgcaggcaaa agctgccaca ctgtttaatg
    17101 acgcccagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gcgggcgatc
    17161 tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg ctgcccaatg
    17221 aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg tgttaagcca tgtggcttgg
    17281 gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag gagatggacg
    17341 aaactatttc tcctttagcc gccaacatgg gctggactat cgcctgggaa ccggcagatc
    17401 gtgactttat cggtcgtgaa gccctggaag tgcagcgtga gcatggtaca gaaaaactgg
    17461 ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgctttaccg
    17521 atgcgcaggg caaccagcat gaaggcatta tcaccagcgg tactttctcc ccgacgctgg
    17581 gttacagcat tgcgctggcg cgcgtgctgg aaggtattgg cgaaacggcg attgtgcaaa
    17641 ttcgcaaccg tgaaatgtcg gttaaagtga caaaacctgt ttttgtgcgt aacggcaaag
    17701 ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa
    17761 atacagcaaa gaacacgaat ggctgcgtaa agaagccgac ggcacttaca ccgttggtat
    17821 taccgaacat gctcaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg
    17881 cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcagtaaaag cggcgtcaga
    17941 catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga gcgattcccc
    18001 ggaactggtg aacagcgaac cgtatgcagg tggctggatc tttaaaatca aagccagcga
    18061 tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt tagaagacga
    18121 gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaaaatt tatgcctcac
    18181 cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
    18241 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
    18301 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggcgcacaa tcgttaaacg
    18361 cgctgaccgg ccagattgta ccgaaagata ttcagcttgc gaccccgccg caggttggcg
    18421 caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac
    18481 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
    18541 gtaacatgct ggaaaatccg ggctggtata ccgcgtacac tccgtatcaa cctgaagtct
    18601 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttgactggac
    18661 tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga
    18721 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccgatgtgc
    18781 atccgcaaac gctggatgtg gtccgtactc gtgccgaaac ctttggtttt gaagtgattg
    18841 tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg
    18901 taggcactac cggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca
    18961 aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccgggta
    19021 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg
    19081 gtggcccaca cgcggcattc tttgcggcga aagatgaata caaacgctca atgccgggcc
    19141 gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga
    19201 ctcgcgagca acatattcgt cgtgagaaag cgaactccaa catttgtact tcccaggtac
    19261 tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccggttggc ctgaaacgta
    19321 tcgctaaccg cattcaccgt ctgaccgata ttctggcggc gggcctgcaa caaaaaggtc
    19381 tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg
    19441 gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc ctgaacgccg
    19501 tggggatcac ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt ttcagcgtgc
    19561 tgctgggcga taaccacggg ctggagatcg acacgctgga caaagacgtg gctcacgaca
    19621 gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
    19681 atcgctacca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
    19741 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
    19801 ccgagatgat cccaatcacc tggccggaat ttgccgaact gcacccgttt tgcccgccgg
    19861 aacaggctga aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga
    19921 ccggttacga cgccgtttgt atgcagccga actccggcgc acagggcgaa tacgcgggcc
    19981 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
    20041 tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg
    20101 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg
    20161 aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt
    20221 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
    20281 ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt attggcgcgg
    20341 acgtttcgca cctcaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg
    20401 gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg
    20461 tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg
    20521 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
    20581 aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg caggatgcct
    20641 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
    20701 gcccgctgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact
    20761 acggtttcca cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
    20821 ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg gctatccgcg
    20881 cagaaattga ccaggtgaaa gccgctgtct ggccgctgga agataacccg ctggtgaacg
    20941 cgccgcacat tcagagcgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg
    21001 cggtattccc ggcaggcgtg gcagacaaat actggccgac ggtgaaacgt ctggatgatg
    21061 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca
    21121 ctgattcgac tattttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa
    21181 aagtgctcag acagtcccct cgcccctttg gggagagggt tagggtgagg ggaacaggcc
    21241 ggcactggcg cgaatattta ccctcacccc ggccctctcc ctgaaagggc gaggggaaaa
    21301 gcgtgccaac attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat
    21361 taatttttct cggaaaaaca aatggctata gcacttgtga ccgatggcag tcgcggcatc
    21421 gggcgggcaa ctgcattact gttggcgaaa gaagagtata cggtggcggt taattatcaa
    21481 caaaatctcc atgcggcgca ggaagtggtg aacttaatca cgcaagccgg tggcaaggca
    21541 ttcgtgctcc aggcggatat cagcgacgaa aatcaggtca tcgcaatgtt tacagcaatc
    21601 gatcagcacg atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag
    21661 tgcaccgttg aaaaccttac cgcagagcga atcaaccgag tactttccac caacatgacg
    21721 ggatattttc tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt
    21781 ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat
    21841 gttgattacg cggcatcgaa aggggcgatt gatacgttaa ccaccggact atcgctggaa
    21901 gtcgccgcgc agggggtccg cgttaactgc gtgcggccag ggtttatttt taccgaaatg
    21961 cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag
    22021 cgtggtggac aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc
    22081 tcttacgtca cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc
    22141 tgaccggatg caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg
    22201 ctggtaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
    22261 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggtg
    22321 gtgaacgata cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacgccatcg
    22381 taagtcaccg cttttttcat ctcttcgata tgggcgcgca ggtagtcaat gcggtagtcg
    22441 tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
    22501 aacagcggcc tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga
    22561 tcaatctgcc agccccagtc agacgcttta acatacgggt tcggtacgct gccttcgaaa
    22621 ccagagatcg catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg
    22681 aaaccaagat aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg
    22741 atgttaaatc cgcgacgctc ccactcgttc aacacatagg acgggtaata gccgcgtagc
    22801 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc
    22861 gggttacagc agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc
    22921 gggttaatgc gacgcgcagc tttcaccgcc agggcgctgg cgacaaactg gtgatgcagc
    22981 acctgataca tcgtctcttc cgggttctca tgctcggtat aaaccacgcc ggagcagcag
    23041 taaccgaaca gcggtgcacg ccagttacgc tggttgttaa tttcgttgaa ggtcatccag
    23101 tatttgactt tatgcttata gcgttcaaac accacttccg cgaaacggac aaagaaatca
    23161 accactttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
    23221 tgggagaggg tgatcaccgg ttcgatgttg tatttcagca gttcatcgaa catatcatcg
    23281 tagaacttca gcccttcttc gtttggctga gcttcatcgc cttttggaaa aatgcgcgtc
    23341 caggctatgg aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
    23401 ttgtagtgac cataaaaatc aacggcttca tggtttggat agtatttgcc tggcaagact
    23461 tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc
    23521 ggccctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac tgcgccgccc
    23581 cataagaaat ctttctgtaa ggtgagtttt ttcactatca taggctccag atgagtcaat
    23641 tcttacagga aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga
    23701 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
    23761 aacgggtgat gtgcttcgtg tcgggcgttt tgaagatgac ggttattttt gcacgattga
    23821 agtcaccgca acctcaaccg taacgctgga tacgctgacg gaaaaacatg cagagcagga
    23881 aaatatgacc ctgactgaac tgataaaggt cattgccgac atctatcccg gtcagacaca
    23941 gttttatgtg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
    24001 cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac
    24061 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
    24121 ctccggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
    24181 aaattgccaa cagcgtcagt cacggcattg ggttggtgtt tggtatcgtt gggctggtgt
    24241 tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc
    24301 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
    24361 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg
    24421 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
    24481 tgattgttat ctggagcctg gcattgctgg gtattctgtt taaactgacc atcgcgcacc
    24541 gattcaaaat tttatctctg gtgacctatc tggcgatggg ctggctgtcg ctggtggtaa
    24601 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttattggcg gtaggcggcg
    24661 tggtttattc gctcggagtg attttctatg tctgcaaacg cattccctac aatcatgcca
    24721 tctggcacgg cttcgtgctc ggcggcagtg tgtgccactt tctggcgatc tatttgtata
    24781 ttgggcaggc gtaatgggta tgcctttgta ggcctgaaaa gacgcgtcag cgtcgcatca
    24841 ggcatcggag cacaactgcc ggattcggcg tgaacgcctt atccgatcta cgggcctcgt
    24901 ccctgttgtg ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag
    24961 gcgataaatg cctgatgcga taaagagatt tactcttcga gcgaatacgg cagcggctca
    25021 atatgcaatg tattcgcatc gtcgcgtacg cggaagatgc tatccggttc catatcgtta
    25081 ttcatgacga cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta
    25141 ccggtacgac gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt
    25201 cggctggcgc tacctgccag caaccagagc gcacgtttat tggcaccacg gaattttgct
    25261 cgcgccacca tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc
    25321 tggagattgg tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa
    25381 cccgcttcaa tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc
    25441 agtttatcgg tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc
    25501 gggtattcaa accatagcaa agtggtcgcg ccttctttaa ctacctgttt ttctttcgaa
    25561 ggcagttcac taaagatatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc
    25621 agcacatgct cgtcgtctgg cgcgatggtc actttagaga acaccgcata ttttttcagt
    25681 tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc acgcaaagcc atcgccgtcg
    25741 cggaacagac gtaaattgct ccacattttg cctttggcgt cgcaatgggc ggcgagcagg
    25801 tgctgatctt ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg
    25861 ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt
    25921 aaacgggcag aagccgtagg ctgacgggga ggaaaaggtg taaaagccat aatagagtcc
    25981 tgagttgctt aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg
    26041 ctttcactac gcgatctgtg ccttctgcga ggcgacttcc aaaaaaatga agccattgaa
    26101 ttgttgtgaa ttaatttcgt gatcgcgatg gtgtttactg atattcccgt aaaagaaggc
    26161 gaaaaacacg ttacaatagc ggggtactgg attttcgtga gacaggaaga agatggacat
    26221 taacaacaaa gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc
    26281 aatcatgccg tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt
    26341 tattcgtctg ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa
    26401 accggccgat gcagaactgg aaatgatggt ccgactcatc cagacacgga accgggaacg
    26461 tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga
    26521 ttcatgggct ggttgccgct gttattttac tcatgccctg gccactcagt tacaccccgt
    26581 tatggatggt gttactttcg ctggtggtgt ttgattgcgt tcgcagccag cggcgtatta
    26641 atgctcgcca gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg
    26701 agtggagcat cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt
    26761 ctgatggcgg taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat
    26821 ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa
    26881 gcatatgcgc taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt
    26941 tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc
    27001 gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat
    27061 ccttccgttg gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc
    27121 ataccgagcg catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc
    27181 gcaacaattt taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct
    27241 tcctggattt caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc
    27301 attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata
    27361 tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc
    27421 atattcataa cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc
    27481 gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag
    27541 tgaaacagga tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc
    27601 tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc
    27661 atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag
    27721 aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc
    27781 gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg
    27841 cttcaccgaa attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt
    27901 tattacaggc gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag
    27961 tgttgtatgc taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca
    28021 gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtaccgt
    28081 taggtgaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc
    28141 tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac
    28201 agaccttctg gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc
    28261 tgtcaccgca tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt
    28321 tacgcgtggt gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc
    28381 atgtcgctac cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc
    28441 tgaaaagaac gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt
    28501 tttcaggttt tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca
    28561 tcaaaagcag cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca
    28621 gcggcgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg
    28681 ttagcggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc
    28741 ttgaaaaaga gatgatcgtt tataaagcgc tgcaggaaaa acacgtcatc accgtgttta
    28801 ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc
    28861 tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga
    28921 aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg
    28981 caggtaaaag cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg
    29041 gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc
    29101 cgggttacca gccgccgaaa gagatgaaag aattcctcga cgaacaccaa aaaatgacca
    29161 gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgtgcag tcgatgaaac
    29221 ggcagacttg cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggcgt
    29281 gcgcagtgcg caagaactgg aacgcagtgt taaagggatg ctgccctggc agcaactgag
    29341 cggcgtcgaa aaggccgttg agatccttta caacgccttt cgcgaaggaa cgcggattat
    29401 tgtggtcggt gatttcgacg ccgacggtgc caccagcacg gctctaagcg tgctggcgat
    29461 gcgctcgctt ggttgcagca atatcgacta cctggttcca aaccgtttcg aagacggtta
    29521 cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt ggtgcgcagt taattgtcac
    29581 ggtggataac ggtatttcct cccatgcggg ggttgaacac gctcgctcgt tgggcattcc
    29641 ggttattgtt accgatcacc atttgccggg cgaaacatta cccgcggcgg aagcgatcat
    29701 taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc
    29761 gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg
    29821 cggcatcgca attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtggc
    29881 ggacgtcgta ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat
    29941 ccgtgccgga aagtgccgtc cggggattaa agcgctgctt gaagtggcaa accgtgatgc
    30001 acaaaaactc gccgccagcg atttaggttt tgcgctgggg ccacgtctca atgctgccgg
    30061 acgactggac gatatgtccg tcggtgtggc gctcttgctg tgcgacaaca tcggcgaagc
    30121 gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg
    30181 aatgcaggtt gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc
    30241 cggcgggctg gcaatgtatc accccgaatg gcatcagggg gttgtcggta ttctggcttc
    30301 ccgtattaaa gagcgttttc accgtccggt tatcgccttt gcgcccgcgg gtgatggtac
    30361 gctgaaaggt tctggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt
    30421 agacacactc taccctggca tgatgctgaa gtttggcggt catgcgatgg cggcgggttt
    30481 gtcgctggaa gcggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga
    30541 gtggctggac ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc
    30601 cgaaatgacc atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt
    30661 cccggagccg ctgtttgacg gtcatttccg gctgctgcaa cagcggctgg tgggcgaacg
    30721 tcatttgaaa gtcatggtcg aaccggtcgg cggcggtccg ctgctggatg gtattgcttt
    30781 taatgtcgat accgccctct ggccggataa cggcgtgcgc gaagtgcaac tggcttacaa
    30841 gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg
    30901 gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt
    30961 atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt
    31021 aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact
    31081 acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct
    31141 ggaatgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg
    31201 tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg
    31261 ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg
    31321 aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg
    31381 actgctacct cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca
    31441 tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg
    31501 aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt
    31561 acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aagagcccgt
    31621 ttgactccgg cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg
    31681 atgatgatat tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt
    31741 ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc
    31801 cgaccgggat cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg
    31861 ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga
    31921 aacaggcgat ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg
    31981 tccttgatga ctcccgcatt aaagatctgc gtaccggggt agaaactcgc aacacgcagg
    32041 ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag
    32101 gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg
    32161 aactgaaaac gcgtcgtgag aagctggcga acctgcgcga gcaggggatt gccttcccga
    32221 acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga
    32281 acgaagaact ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc
    32341 gtattatggg taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt
    32401 acgttgcccg tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc
    32461 tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta
    32521 tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc
    32581 acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg
    32641 atgaatcccg taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca
    32701 tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg
    32761 ccgctgcgcg tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta
    32821 tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa
    32881 tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga
    32941 tggaactcta catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt
    33001 tccgtactct ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc
    33061 tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc
    33121 cggaaaccga catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta
    33181 tcggtatcca cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag
    33241 aagtggcaga agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt
    33301 ctccgctggc gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca
    33361 tcggtggtcg tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc
    33421 aacgcttcct ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg
    33481 atgaagatta cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg
    33541 gcatcgaccg tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt
    33601 tcccggcgat gcgtccggta aaataagcat tacgttatgc tcacaacccc ggcaaatgtc
    33661 ggggtttttt aattgtgctg cgcgaaagca gacaacaatt ttcttgcttc actattggct
    33721 gcctgcatca ccatccacgg actgaacgcc cacggtgtgg catcaatacc gtgtaataca
    33781 tctgctaaat cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg
    33841 gtggtgcgtg cggcaaatac cggacacact tcattttcca caatgccatt cggatcggtg
    33901 gcgcgatagc gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca
    33961 taacggcaac ggcggatcac cgcgtcttcg ttgctttctc ccagttgtgg gtgcccacaa
    34021 accgagttag tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat
    34081 aattgtcctt tggcattaaa cagccaactg gagaacgcga gatgtaagcg ggtgtctgcc
    34141 gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg
    34201 acgtgttccg tttgcataat ttctcacatg taattctgat cactcatcat tatagataat
    34261 tgaagagtga aatgattaac caagtgagcg tttatcgaca accgcccgtt ttgagcggat
    34321 gccgacaggt aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg
    34381 ccattgcaac acagtcagaa atgacaggaa tagagagagt aaaaagcaac agcagactga
    34441 atataacgcg cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc
    34501 atcagaagcc acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc
    34561 accaggtcag catgatgctg gtagccatta aagaagacat tcagcacaac ggcgctaagg
    34621 gttgcgagca taatgccgct atgcagcaac ggttgcagta cggccggtaa cttagaaaag
    34681 aaatcgtgag agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg
    34741 ttgtaacggt tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc
    34801 atgccgaaca tcaccagccc agcaccgccc agcacaaatt gcggaatgga ggcgaccagc
    34861 accgccattt ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc
    34921 cagcggctat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacagcaa
    34981 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccggcggccc
    35041 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    35101 gtggcgggaa taaaggcatc aagcgaccaa taaatggcgc taataatgtg gtgataaaac
    35161 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    35221 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    35281 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    35341 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    35401 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    35461 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    35521 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag agtaaaaagc
    35581 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    35641 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    35701 aatgacaaga gaacctgaca atggaattat ctgtaatttt ctcatattga tatttcatta
    35761 atcattctgg catgtcgcac tctagcattt aatcgttttt atctggatag cgctcttttg
    35821 atcggcaatg ttgaaataat gaaagcggca tagattgagg ggcaataatt tgaactcagt
    35881 cgcaaaatta taattaaaac ggtcatgcta cctaatatat gcccgtttat tgcatcgcga
    35941 aatttgagaa gacataactc attaaatgaa agggatgcaa atgaataagt ttatcgctgc
    36001 tgaagctgcg gaatgtatag gctgtcatgc ttgtgaaatt gcctgtgcgg tggcacacaa
    36061 tcaagaaaac tggccgctga gtcacagtga ctttcgaccg cgtatccacg ttgtagggaa
    36121 aggccaggct gcgaatccgg tggcctgcca tcactgcaac aatgcccctt gtgttacggc
    36181 ttgtccggtt aatgctctga ctttccagtc cgatagcgta caactggacg agcaaaaatg
    36241 tattggctgt aaaagatgcg caatcgcttg cccctttggc gtcgttgaga tggtcgatac
    36301 gattgcgcag aaatgcgacc tttgtaacca gcgcagttcc ggcacgcaag cctgtattga
    36361 agtctgccca acacaggcgt tacgactgat ggacgataaa gggttacagt ggataaaggt
    36421 ggctcgccag cgcaaaacgg cagcaggaaa agcgtcatca gacgctcagc catctcgcag
    36481 tgcagcgttg ctccccgtta actcgcgtaa aggcgcagat aaaatttcag cgagtgaacg
    36541 gaaaacccac tttggcgaaa tctattgcgg gctggatcca ctgcaagcga cttatgagag
    36601 tgaccgctgt gtttattgtg ccgaaaaagc taactgcaac tggcattgcc cgctgcataa
    36661 cgctattccg gattacatcc gtctggtaca ggaaggaaag attattgaag cggcagaact
    36721 ttgccaccag accagttcct taccagaaat ctgcggcagg gtatgcccac aggaccgtct
    36781 ttgtgaaggc gcatgtactt tgaaagatca ctctggcgca gtctctatcg gtaatctgga
    36841 acgctacatc accgataccg cgctggcgat gggctggcgt cccgatgtca gcaaagttgt
    36901 tccccgtatc gaaaaagtgg cggtgattgg cgctgggcca gcagggttag ggtgtgctga
    36961 tattctggcg cgcgcgggag ttcaggttga tgtctttgat cgccatccag aaattggcgg
    37021 tatgctgact tttggcattc ctcctttcaa actcgataaa acggtattaa gccagcggcg
    37081 agagatattc accgcaatgg gaatcgactt tcatcttaac tgtgaaattg gccgcgatat
    37141 tacctttagc gatttaactt ctgaatatga tgcagttttc atcggcgtgg ggacttacgg
    37201 gatgatgcga gcagatctgc cgcatgaaga tgcgcccggt gtcattcagg ctctaccgtt
    37261 cctgactgcc catacccgcc agctcatggg attgccggag tctgaagagt atccgctgac
    37321 ggacgtggaa ggtaagcgag tcgtggtatt gggcggtggc gatacgacaa tggattgttt
    37381 gcggacttcc attcgcctca atgctgccag cgtgacctgc gcgtatcgtc gtgatgaagt
    37441 cagtatgccg ggctcgcgca aagaggtggt taatgcgcgc gaggaaggtg tcgagtttca
    37501 gttcaatgtt caaccgcaat atatcgcttg tgacgaagat gggcgtttaa ctgcggtggg
    37561 cctgattcgt accgccatgg gtgagccggg gccggatggt cgccgtcgtc ctcgtccggt
    37621 agcgggttca gagtttgaat tgcccgccga tgttctgatt atggcctttg gtttccaggc
    37681 gcatgccatg ccgtggttgc agggcagcgg aattaaactc gataaatggg gcctgattca
    37741 aaccggtgac gtcgggtatt tacctaccca gacgcatctg aaaaaagtct ttgctggtgg
    37801 tgatgcagtt catggcgcgg atctggttgt cactgcaatg gccgcaggaa ggcaggcggc
    37861 gcgcgatatg ttaactctgt ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt
    37921 aatccggctg actgtattgg ctgccgtact tgtgaagtag cctgtgtggt cgctcaccct
    37981 tcagaacagg agttgaatgc cgatgtcttt ttaccccggc tgaaggtaca gcgactggac
    38041 agcatcagtg cgccggtgat gtgccatcag tgtgaaaacg ccccttgtgt tggcgcttgc
    38101 cccgtggggg cgctgacgat gggcgagcag gtggtgcaga ccaattctgc ccgttgtatt
    38161 ggctgtcaga gttgcgtcag tgcgtgcccg tttggaatga tcaccattca gtcgttgccg
    38221 ggggataccc gacaacaaat tgtgaaatgc gatctctgtg aacagcggga agaggggcca
    38281 gcctgtgttg aatcttgccc gacacaggcg ctgcagttgc ttactgaaag agaactcagg
    38341 cgagtccgcc agcagcgtat tgttgccagc ggtgagaatc ctctgtgaaa gaaatctccg
    38401 cgctgaaagg cgcggagaat ctattagatt gcccaaccac ccgcgtaaaa tgcgaccagt
    38461 gcgacaggaa cgctaatttg ttgcccgagt accagactga atgcggtaaa tgccgtcaag
    38521 gaaatcgcac aaccaatcgc cattggcaaa ttggcctaca accccatcag caacgagccg
    38581 aagcccgcga ccagacaggt ggcaacaaac acagctgcgg gaggaaaacc tgctttgccc
    38641 agcattcccg gaacgacgat aacggaataa accatggcca gaaaggtcgt taagccagcc
    38701 agtacttcct gacgaacggt actgccacga gcggtaattt taaaataatt atccagcgcg
    38761 ccctgtggct ttggtgcgtc cggtgtttgt aggatgtctc cagacataat gatgtcctca
    38821 cgaggatgtt aaagttttat tattagttgc gttcgtacac cagacgacca tcaacgtagg
    38881 tgcggtagat cgaacggtca tcgcccaacg tcatcatcac gaacaatttg tcgactaaag
    38941 aaacagagtt gtcatagcgc agctgctgta gcggagtggc ggtgggttcc atcaccacga
    39001 aatcagcctc tttgccaggt aaaaagttgc caatcaaatc gtcaaggccc agagatttcg
    39061 ctccgccgag cgtggccagg taaaacgctt catatgccga gaggcgatag ccttgtaatt
    39121 gcaacacttt gtaggcttcg ttcagcgttt gcagcatgtt gaaagttgtt ccggcaccga
    39181 tatccgttcc catgcccact ttgactttct tctgcccaga tgccgcatgc atcgtgaatt
    39241 gcggtagcaa tcgccggagc tgcgccattt acaccgattt ccgagatcga tttcgccccg
    39301 aacgggccga ctttatcgtc gctcggcacc agcacagcgc ggaaatcacg cggaatgtca
    39361 ccaattttcg gtgcgccgta actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca
    39421 taaatgatct cttcgctcat actgtggccg atagctcgca gggtagcacc gtagatttgt
    39481 cccaatgcta attccgggtt gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc
    39541 aggcggattt cacccgtacg cgtgttgacg gcaacttcag cgaagtttgc gccatacggg
    39601 aaggcgaaat caggcgtgat ataactgcca gttcccacca gtgaaccaaa gccgatgccg
    39661 gtttcgcctt tatgggcaat atccccgaaa ctaacttcgc ctttcttgcc gcgcacgacg
    39721 cccggcgttg ccagttgaac atctgccact gcctcaccta acatttgcgc gccgtggaac
    39781 agaattttct cccgtagatt ttccgctgcc aaacgcgccg cgttacccga gaagcaagta
    39841 ccggacgagg catatgcgcc tttatcaaac aacgcatgat cggtatcacc ggagataaca
    39901 tgcacgtcct gcggtgggca gtgcagcact tctgctgcca gtttcgtcac tacagtatcc
    39961 agaccagtac caatatccgc accgccagaa tgaacgataa aggtgccgtc cgattccagt
    40021 ttgatcatgc agttagcctg atcgatatcc gggatacccg atttctgcat gataatggcg
    40081 acgccgcgac cgatgtgcca gtcaccattt tgcggtttcg gtgacgacca ttggatcatc
    40141 tcgcgaccct gacgcaggat ctcttccagt gcacagctgg cggcggaagg aacggaggtc
    40201 ggcgctttac cttcaccgat tgcaccgaga attttcagct cttgcccttc gtgtacccgg
    40261 ttacgttcga taatttccag ttggtcgatc tgtaactgtt cagccagttc cgctaatgcc
    40321 atggtgatag cgaagttacc tttcggtgcg ccataaccct gataagcacc atttgggcaa
    40381 atgttgctgt agtaggtggt gacctggaaa tcgacgttat cgcacggata taacggcagc
    40441 gacagcgccg gtccgttaca cggtacggtg agtgagtggt tgccgtaagg gccagtgttg
    40501 gcgcggaaat ccatcttcac tgccgtcagg cgaccatctt ttttcgctcc cagtttgacg
    40561 gtgactttcg cgacgtgacg agaggtgtta gcaataaact cttcttcacg ggtgtagcgg
    40621 aatagtaccg gacgcccggt cacgcaggtt gcccaggcgc acacttcttc cagcaggatg
    40681 tcctgtttgg aaccaaaacc gccgccaact cgctctttaa tgacatgaac tttatgctgt
    40741 ttcatgccca cgaggcgcgc gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg
    40801 tggataacca gacgatcgcc gtccatccgg gtaaagcaga tatgtgtttc agtcgggcac
    40861 tgctgtgcct gcgttgagtt ataggttcgc tcaatgatca catcggcatc ggcaaagcct
    40921 ttgtccatat cgccaatatg accatgaata ctggcggcga tatttttgcg agggcgagaa
    40981 ccgatcggga agttgatgat catatgctcg ccgcgctggg ctgcatggct gttatcgtct
    41041 tccagagtat ctggcgcacc agcaacatac accaccggtt catcgtgcac gacaggcgca
    41101 tcttccgcca ttgcttcgtc gatcgacatt accggcttaa gcacttcata ttcaacgtcg
    41161 atgagcttca gtgcttcgag cgcaatttct tcactttctg cgacgaccgc agcaacacga
    41221 tcgccgacgt gacgcatttt cttgccgaac atacggcggt caagcggtga cggttccggt
    41281 gcgctctgac cccccggggt atagtagata tcagggcaat tcaggtgagt aataacgtga
    41341 acgacgcccg gtaaggcttc agctttgctg acatccagat gagtaatcag tgcgtgagcg
    41401 tgtgggctac gtaacatttt aatgacgcag gcgtcagccg ttacgcagtc ttcaacatag
    41461 cagggtttcg cctgcaccat tttcgcggca tcagttttag gataatgctt gccaatgacg
    41521 tcaaggtcgt cacggaaagt cggagcgata tcaatggtgg cctgcggatt atttttacgt
    41581 gcaaccgcca gttcaatgac ctgatagtat tgttgccagc ctgcatcgcg gctgaacaaa
    41641 ccagaaagcg cgtcgtcgat ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcg
    41701 gtgataatca gagctgcagc tggatcgtta taaccagact gcaccacgcc aacatcaacc
    41761 attgcctgtt gaaccagact taactcgttc catttaccca gagattctgc ggtacgaata
    41821 tctgccttct ctaactgtgc ggcaataagc aaggacgcgt taacgatatt accgttaaag
    41881 attattgcgt cagaaccggc aaacccgaag ccatcatcac tgttgcgtac agagtgcatt
    41941 cccatgttaa acaacagctt ttgcacgttt tcgcctggat taacggttag ctcctgaggc
    42001 gcgccattta aagtaaagtg gatgatcata cggcttcctc cccagcctgt tggcagtcgg
    42061 catacaggtc ggctaccaga actcccgtga tatagcgttt ataggccacg ctgccccgca
    42121 aatcttcctg cgggaaaatg gcgttggcga cagcctgttc aagtgcattg ccttccagat
    42181 tttgtttttc gacatcgtgc agacgcagtg ctttactggc gacgccatcc agcgcaattc
    42241 gcataccgtc gtggtctgtc attgcaacgg ctgccgtcac gacggttaaa cctgcctgag
    42301 aacggctaat tttgcgggtc gcacaggtgc gatacggatc tttaatgata atttcggtta
    42361 gcaggcgatc gcatgggcag gccaggtagt cctcgattga cagcgtttcg ccgttgccaa
    42421 aaaccagttc agcatccagt gctagcagga cgggaagcag cactgactct tcctgccggg
    42481 cggcgatttc accaccaatg gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga
    42541 gggcttcacg cagtgctgca ggaataaagc gcgcatcacg cagtggctgc aagcgagaca
    42601 ttgcaccaat ccgcagtgca ccgttatccc agtcaaccca gtccagttcc agatcctgta
    42661 aggaaatggc aatcttttta tcggtacggg ttggtgtagc attgagtttg ctgcccccgg
    42721 cgaaccagac ggcttcatcc tggtagcggc gcttcagttc cagcgcctgt tcgacagagt
    42781 cgggcctgaa aaattgttca atcatcttct ttccttattt aaccctcttc cctgcgggaa
    42841 gagggcatct gtcatttatg ccagcgcatc catccgacgc cacatactgg cagcggcttt
    42901 tctggcttgt gcataaatgg aatcgcaatc gaagttaaac tgacggtctt catagaccat
    42961 cacaccattg accatcacgc tgtgaacgct gcctgagccc ataccgaaag cgatatgccc
    43021 ggcgatattg tctgccagca gcggcgtcgg cgagttgtaa tcgcaaatgg tcaaatcagc
    43081 tttgtaaccg gcttccagaa gcccaaattt cgcgccaaag ttgcggctca tcagttcgtt
    43141 accgttagtc agggctttgg caaaactgtc aggccacagc ggaccacccg catcgcgatg
    43201 tttaaagaag gcaaatttca tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa
    43261 tgccaggttg cggatgtcgc taaggtgatg gttgtagccg acatggttgt tcatgtttga
    43321 acgggcgtta tgcaccagga acgcatcgcg ctgattgagt agggcgatgt catctttcga
    43381 caagtacagc ccatgagcga ccagcgtttt gctgtcgatg aggtcgaatt gcgccagtcg
    43441 tgccagcagg tctttgccgt accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc
    43501 agcgtgaata tgcaaaccac ggcctgtggc tttcacggct tcacgcagca tctccagacc
    43561 ggcatccggc acggtaaacg gcgcgtgagc accgatatgt gcttccacca gatacggctc
    43621 gcttgtcgct ttcttcgcct catcaatcaa acgggcgaaa cggatgtttt cttctacacc
    43681 ttcctgcaac tctttgatgc cgttgttacg gtcagtagtt tcaaaacagg tcatcgcgcg
    43741 caggccaact tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga tatacgccgg
    43801 cgaggcatgg tgatcgataa ccgatgtaca tccgctctta atcgcttcca gggaacaaat
    43861 cagtccgctg taatagagcg actcttcatc aagggcgcga tcgagccgcc accagagatt
    43921 tttcagcgtt gagatgaaat ccgggcaagg ggcgatgttt gccataattc cgcgggaaag
    43981 ctccgagtaa aaatggttgt gcgagcagac aattcccggc atcacaatcc gaccatgcat
    44041 ctctttgtag ctggcatcgg ggtagcgttg cgtcagggca tcgccgatag cgacaatcac
    44101 gtcattttcg atagcgatat caacgccttc ctgcactttc gccgggtgta actgcactgc
    44161 ggtgacattc ttcagaatca acatgattac acctccacgc ggcccagcag ataatgatga
    44221 tgctggtgga catggctgat gatgcggcac atatcgttca gctccggtgg tacgttgtta
    44281 aactgacctt cgctgtcgat gtttaacacc cagctttggt tattcagacg tactcgtacc
    44341 cggcaatctt ccacaaggaa gcctggggtg ctgctgttat caaagtcttg cgccaggctg
    44401 aagacggtga ttttgtcttc gtatggttta ccgttccacg gacagaactg agcgcagttg
    44461 ccgcattcgt tacagtaagc gtcgaggtgc agcgtctgga atctattctg gaagccaggg
    44521 actgcaatgg agacgttggc gcggttcggg cagacatcca cacacttgtt gcaaacgtag
    44581 ttacattcga ggcagcgagc ggcttcctgg gcgacaaacg cgtcacgatc gtcactgttc
    44641 accagcgtga tagagatatc gcctttacgt tgatagattt ccgctggatt gacgttgttc
    44701 cagtatttat cgttctggtg ggaacggata ttttcccgac taaggatggc atcggtcgcc
    44761 cgacgcgcgg ttccgacagc agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc
    44821 ataaagacgt cagtcagacg agtttcgcca ttatggtcga cgtctggcca gccgtttttg
    44881 tccagcggca cgcccatcgc attcagggct tcagtatcct gctgttcacc aatggcggtg
    44941 atcaggctgt ctacatgcag tgttactgtt tcgttggttt caaccggacg gcgacgacct
    45001 ttctcatccg gttcgccaag cgacataacg cgcaaggtta aggtgccatc agcatcgaaa
    45061 cgttccggat tattcaggaa acggaactct acgccgtcgt gcaacgcttc ttcatactct
    45121 tcgcgccatg cgggcatctc ttgtagtgaa cgacggtaaa cgatcgttgc tttttctacg
    45181 cctggaacgc gtaacgccgc acgagcgcag tccattgcgg tgttacccgc cccgacaacg
    45241 accacatgtt tgcccagctt gagcgctgta cccttgttgt attcacgcag gaaggggagt
    45301 gatttccaga cattttggtt gtcgcccgcc agtttcacac cgctattttt atcagtgccg
    45361 gtggcaatca gaacatagtg gaagccctga ttttttaact gttcaacggt taaatcgggt
    45421 gagcagccat actcaaattt cacgccgtga gcggcaacaa aatcgatatc gtgctgaatt
    45481 aactctgcag gaatacggaa ctgaggaatg atatttttca ccacgccgcc agcattggct
    45541 tcgcgttcaa acagcgtaac cggatggccc gctctggcaa ggaagtaacc tgctgccaga
    45601 cccgccggac ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc ggcgggtttg
    45661 tgccagcgtt gcttatattc atcccaacct ttttccagcg cgactttttt cagttcgcgg
    45721 atattcagcg cactgtcgta atccaggcgg gtacagttgt attggcactg gtgatcgcaa
    45781 atatgaccgg taatggcagg tagggcgttg cgttggtaga tgagttccag cgcgtcggca
    45841 tagcggtgtt cgccaagcag acggatgtat tccggaatat cttgcttgat agcgcaggca
    45901 gtaacacagg gggcaacgta gcaatcggtc agcggcaggt cttctgccac ttcaatacgc
    45961 tcttctggct tccagtgttt ctgggtgtat tccatggtta acgcgtctgc tgccagtctg
    46021 ttcagtcgtt cgacgtcaac atggtcaagt ccccaggcgt cggagccttc cagctcgcgc
    46081 atgcaggcac ttaagcgcag atagccgcca ggtttcagca ggtcggttgc catagtaata
    46141 gggcgaatac ctgtatcaaa aatatcgcgg atagtcagct gactggcacc accggaataa
    46201 gaaatgggca gtttgccgtc aaaggcgcga gagagaactg ctgcaacatt gatggagagc
    46261 gggaacagcg cacggcctga catatacatc tcttcaccag gcagtgcgcc tttattgttg
    46321 atggtgccga gagtgttagt cagttttacg ccaaagccga gtgatttttc ttttgccagt
    46381 accatcaggc gctccagcat ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac
    46441 tcttctttta agcctatgta accgaaaccg cagacatcga ggatctcacg aacacgcgcg
    46501 taccccagta aggtcgggtt aagtttgaca aaggtgttga gccctttttc ttccagcatg
    46561 tagcggcaaa tggcttcgat ttcatgcgga ggacagccgt gcatggtgga gagggtgacg
    46621 ccctgcacca tactggtggg gatgcgagcg ggtaaggctt gcaagttttc gcgtttttcc
    46681 tgcaatccgt ggcgggctaa aaatgcgtca tcctggagta atttattcag cgtatcgcga
    46741 tattgagcga atttcggatg gtcagatgcg tccatcatat tgtcgatgaa ctgttgcatc
    46801 ggcggttgct taataccttc gaggttgtaa ccgacgctca tattaaagat gaacgattta
    46861 ccggaatcag aaggctgtaa catcgcttcg agaaggtgca gggcaaacca ggctttgagg
    46921 tattcatccc aggctttaag caaggtaaat tcggtagacc attcggtgtt aaagcattcg
    46981 tcttcggcat caatacaggg cttttccagc tccaggcggt caagaatttg gacggttttt
    47041 agttcgatga agcgtccgcc agtcagccag gaagtgacaa tattttgcgc gagctgcgtg
    47101 tgcgggccag cggcagggcc gacgggagtg gcacaggttt caccgaagac gctaacagtt
    47161 ttacctttta caggtgagta aaactgttgc tcgggaatac caaagattga gcgttgttgt
    47221 tggtattcat caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc
    47281 cccataaccc tctccttagc ttatacagat atcattgagt gaccggtgtt gagaaatccg
    47341 gtggtttagg gggatagagc aacaatcgca ccactcttgc caggtttggc gggaaatata
    47401 aaaaaatgtg aggtggctcg aaacttaaag gctttttgtg aatgaactca caggtgccaa
    47461 atgagaatta aaatcactcc tgaaaattat tattgtcagc ccatttatca ctttttctct
    47521 tttctcaaaa tcagtgattc cttatcatta tgataaagag tgctggagag attaaatatt
    47581 tgaacgcaat cgtttaacta cattttctaa attttagtat accttttttg cgctgtaata
    47641 aagtcgtccg gagtatcaat atctaaaatt atttctgcat tttctatttc gacggaataa
    47701 tgatctccca taaggagtgc ctgacgcata ttggtgacat tgggtcgctg gattgcctgc
    47761 atcaggcatg gttttgacac taaaatcgga tggccgggga tgccattatg gagtggcagt
    47821 attgcgccat catttcgtaa cgaccagatt tttctaaaaa tatcgatggt gagggttggc
    47881 atgtcaccgt gggtgagaaa acaatgttct gtttgcactg cgggtactgc ggccttgacg
    47941 gatgttagta aaccctgcgc ataatctggg ttgtgaataa tagtgatatt gctctggttc
    48001 gcataacgtt cgtgcagttc attaccacga tagccggtga ctaaaataat tcggctacaa
    48061 aactgcaacg catttttgat acttgtatca agaattgttc cctgttccca ggggaacatc
    48121 attttccatt gccccattct tgatgataat ccggcagcgg taattataca gtcgatggct
    48181 gacatagtgg ttccattatt tataggggct aataacgcct gataattgcg ttacttcctg
    48241 atggattaag ttggggataa tttatcccag agaggtcata aagactcgtg aaaagtatag
    48301 ttgacccatc ggcattagtc attgatttag gtgcgcagaa acgtcccact gtgatttctg
    48361 ttgttggtgc tggggggaaa accagcttgc ttttttggct ggcagaactg ctccaggcta
    48421 gcggcaggcg tgtgttaatc acgacgacga cacatatgtt tatgccaacg tctcactggc
    48481 ccgtggtttt ctgtcgtgat cccgccatgc ttcctcatgc gtctcttaca tcccccattt
    48541 cattttgttt tcacagctgg aaagcgaacc agggaaaagt gcagggattt acgccagaag
    48601 cgattgatgc actggtgcaa cgaccagagt gtgacgtaat tctcattgaa gcagatggct
    48661 cgcgtggaat gccgttaaaa gcgcctgatg agcacgaacc ttgcataccc aaaagcagtt
    48721 gctgcgtgat tgctgtgatg ggaggacata ctttgggcgc gaaagtgagc acagaaaatg
    48781 tccatcgctg gtcgcagttt gctgatatta ccgggttaac acctgatgca accttgcaac
    48841 tgagcgatct cgttgcgctg gttcgccacc ctcagggggc gtttaaaaac gtaccgcaag
    48901 gttgtcggcg agtctggttc attaaccgtt tttctcaatg tgagaatgcg attgcacaaa
    48961 gcgagctcct tcaaccgctg caacaacacg acgtagaggc aatctggctg ggcgatatac
    49021 aagaacatcc tgcaatcgcg cgcagatttg tgaattagcg gcccgggtat tccgggttac
    49081 tgaaaggata tccattgagg ttggtatgaa tattttcaca gaggctgcaa aactcgaaga
    49141 gcaaaattgt ccgtttgcga tggcgcaaat tgttgatagc cgaggctcga ctccccgcca
    49201 ttctgcacaa atgttagtgc gcgccgatgg ttctatcgtc ggtacaattg gtggcggaat
    49261 ggttgagtgg aaggtgattg aagagtcact tcaggcattg caggaacgta agccgcgatt
    49321 attccatgga cgtatggctc gtaacggtgc ggatgctgtc gggtcagatt gtggaggtgc
    49381 aatgtcagta tttatcagcg tccatggtat gcgtccacgt ctggtgttga ttggcgcggg
    49441 gcatgtcaac cgggcgatag cccagagtgc ggcgctatta ggatttgata ttgccgttgc
    49501 cgatatttat cgcgaaagcc tcaatcctga actattccca ccatcaacca cgcttctcca
    49561 tgctgagtcg tttggtgcgg cagtggaaga actggatatt cgccctgata attttgtcct
    49621 gattgccacg aataatcagg atcgtgaagc cctcgacaaa ctcattgaac agcccattgc
    49681 atggttgggg ttgctggcaa gtcgccgcaa ggttcagctt ttcctgcgtc aattgcgtga
    49741 gaaaggcgtg gctgaagaac atattgcccg tttacatgcg cccgttggtt acaacatagg
    49801 tgcggaaacg ccgcaggaga tcgccatcag cgtgctggca gaaatattac gggtgaaaaa
    49861 taacgcgccg ggtgggctga tgatgaaacc ttctcatcct tccggacacc agctggtggt
    49921 gattcgcggt gcgggagata tcgccagtgg tgtggcgcta cgtctgtatc atgcgggttt
    49981 taaagtgatc atgctggaag tggaaaaacc gacagtgatt cgttgtaccg tggcgtttgc
    50041 ccaggccgtg ttcgatggcg aaatgacggt cgaaggcgtc actgctcgcc tggcaaccag
    50101 ctctgcggaa gcgatgaaac ttaccgaacg cggattcatc cctgtgatgg tagatcccgc
    50161 ctgttcattg cttgatgaac tgaaaccgct ttgcgtggtg gacgctattc tggcgaaaca
    50221 gaatttggga acgcgggcag atatggcacc agtaacaatc gcgcttgggc cgggctttac
    50281 tgcagggaag gattgtcatg cggtaattga aacaaatcgc gggcactggc tcggtcaggt
    50341 gatttactct ggttgtgcgc aggagaatac cgatgttcct ggcaatatta tggggcatac
    50401 cacccgacgg gtgatccgtg ctcctgctgc aggcattatg cgatccaacg tgaaattagg
    50461 cgatctggtg aaagagggcg atgtgattgc ctggattggt gagcatgaaa ttaaagcacc
    50521 gttgacgggg atggtgcgtg gcttgttgaa cgacggcctg gcagtggttg gtggttttaa
    50581 aattggtgat atcgatcctc gtggtgaaac cgctgatttc accagcgttt ctgataaagc
    50641 ccgggcgatt ggcggcggcg tacttgaggc gttaatgatg ttgatgcatc agggcgtgaa
    50701 agcgacaaaa gaagtgctgg aagtggctta aatgaaaatg gccggatggt attacccacc
    50761 cggccttaaa attactgaca aatcaccgtt ccggttttgc cttcaatccc ttctttcgct
    50821 ttgctcaata cggtaatcag tgcttcacga cctgcgcgag agcgagcaaa cgaggcggca
    50881 gcttccactt taggcagcat agaacctttc gcaaaatgac cttcttcgat aaagcgttca
    50941 gcatcactta atgacaggcg atcgagccac tgttcattct ctttaccgaa gttaatggcc
    51001 actttttcta ccgcagtcag aatgatcagc atatcggcat caatcatttc tgctaaacgg
    51061 gcgctggccc agtctttatc gataaccgcg ctggcaccac gcagatggtt accttcacga
    51121 ataacaggga taccaccacc gccaacggcg atcaccacct ggcctgcatc taccagagct
    51181 ttaaccgttt ctttttcaat gatatcaacc ggttttggcg aggcaacaac acggcgatag
    51241 ccgcgaccag catcttcttt cagagtgtaa ccctgttttg tcagttgctc tgcttcttgc
    51301 tcggtaaaga acgaaccgat cggcttggtc gggttgagga atgccggatc gttagcgtcg
    51361 acttcgacct gagtaaccag cgttgctaca ggtttattaa tgccccgaga aagcagttct
    51421 tctcttaacg cgttttgtag atcgtaacca atataaccct ggcttaaggc aacacagaca
    51481 gacatcggca gcattggcga gtgtgcttca gtttttgcgg cagcttcaaa ggcttggtta
    51541 atcatgccca cctgcggccc gttaccatga gtgacgataa cttcatgtcc ttgggcaatt
    51601 aaatcaacaa ttgcctgaga agtaattttt accgctttca tttgtccggc caggtcatcg
    51661 cccagcgcat tcccgcccag ggcgagaaca attttcttac tcatttttcc tcactcatca
    51721 ttcttgattg tgtatagatc cctgacagtg gttagagcac gggagggaca aacggtttgc
    51781 gtcgcaggaa acgacctcgc ccggctttgc ctgtaaaggt gccgtcacag aaaatcgttt
    51841 caccacggga taaggttctg acaatcgcgc cctgacaggt aaaaccctcc catggcgagt
    51901 agtcggcgtt gtcgtggaga tggcgatgct gaatttgttg gctctgacgt gggtcgataa
    51961 tcaccacgtc gccatcggaa ccgggcgcta ataatccttt ttgcggccac aggccaaaca
    52021 gcctggcggg cattgcgctg gttaattcaa caaagcgttc cggtgttata cgtcccgtca
    52081 tcacgccact ggaaaacagt aactgcatgc ggttttccac accgggtaag ccatttgggc
    52141 agcgactgaa atcgccttta gaaatttgca ggcgttgagc catcgagaag gtgcagtgat
    52201 cggtcgccac cacgtcaatc gcaccatcgc tgatgccaca ccacagtttg tcctgctcgc
    52261 gtacgttacg cagcggtggg ctaagaatga acttcatgcc atcttctgta tcgtaactgc
    52321 gttcgtccaa caggagatat tgtgggcagg tttcaaccca gactggctgg tgattcgcac
    52381 gggcaagacg cagataatcc agacctaagc cgttagacag gtgcacgata tagagcgggg
    52441 cgttaccggc aatttgtgcc aggttaatca tgcgggcgat ggcttccgct tcgcattcca
    52501 gagggcgact caaggcatga tagcgcggcg cggttaaccc tgcggcgata aattccgccc
    52561 gcttgctggc gatagccgca tcattttccg ggtgcacggt ggtcagcgcg ccggattcat
    52621 gcaagcggcg taatgcctgc aaaacctcgt catcgttgag tttgtattga taggttaaat
    52681 agagtttaaa actgctcagt ccttcctcga ccatcatcgg gatttcgtcg aggattgcgt
    52741 gattaatgtg ctggatcaca ccgtgaaagc tgtaatcgat gaccgcttta tgggcggcat
    52801 aaccacgata aacctccagt tgatggcgta accgacagcc gtttgggcca aatcccatat
    52861 ggtcaataat ggttgttgta ccgccacacg cagctgcgcg ggtaccggta aaaaaatcat
    52921 cacaactgcg cgcgatgccg acatcaatat tgaaatgcgt atggacatcc acgccgccag
    52981 ggaaaacgta acagccagtg gcatcaattt cttcatacgg gagctgcggc gaaatattgt
    53041 tgcccaactg gcgaacaatc ccgctttcaa tcagcaaatc ctgtttggct tgtccatctg
    53101 cgttaacgac agtgccgttt ttgatcaata cgcgcatagc aaactccaaa aaccctccgg
    53161 cgaaccggag gggaataagt gaagagtaat tactctgttg ccagccagct taacgggatt
    53221 gcagcgtaca tcgcggcaca ggtcaccagg tgagatttcc aggttttttc gttaggtgca
    53281 tgcgcttcag gttctttacc cgggccaaag ccgatcaccg gaatgccgtg acggcccatg
    53341 atagatacgc cgttagttga gaaggtccac ttatcaacaa ccggcgcttt gccaaacagc
    53401 ccttcgtagg cattcaccag tgctttaacg gtgaagtgat cttcttccac tttccaggtc
    53461 gggaagtagc attcggttgg gtaaaccagg ccagtccagg acggacggtc gtagttgtac
    53521 atagaaacaa ccgcgttagc tttctgtact gcaggcaggg cgcggatttc gtccagcgcg
    53581 ccttcccagg tttcgcccca ggtcagacgg cggtcaatag agacggcgca gctatctgct
    53641 acagcgcaac ggcttgggga tgtgaagaag atttcagaaa cggtgagggt gcctttgccg
    53701 aggaattcgt cataacccag acgttgggag agttcttgta attcgccaag aatcggaccc
    53761 attttgaaaa tggcgttgtc accgcgttct ggcgcagaac cgtggcagct aacaccctga
    53821 acatcaatac gaatttccat acgaccgcgc tgaccacggt atatctggca gtcggttggt
    53881 tcggtactga ccacaaattc cgggcgaatg ccggattgtt caataatgta ctgccagcac
    53941 agaccgtcgc agtcttcttc ctgcacagta ccggtaacca gcagggtata ttcatcttcc
    54001 agaccgaggt ctttaatgat tttaccggca taaaccatag atgccatgcc gccttcctgg
    54061 tcggaagtac cgcgaccacc gattagctca tcagtttcca tgccttcgta cggatcgaag
    54121 tcccagtttt tgatgttgcc aatgccgacg gtatcgatat gagcgtccat tgccaccaga
    54181 cgcgggccgt ggccgatata accgagaacg ttgcccatcg ggtcgatttc aactttatcg
    54241 aagccgactt tttccatctc ttctttaata cgatgtacta cgcgtttttc gtcgcagctt
    54301 tcactgggaa tagcaaccat gtcgcgcagg aagcgagtca tgtccgcctg gtaatctttt
    54361 gctttttcaa gaatcagttt gaatggaata ttcttagcca ttatttttct ccagattttt
    54421 tactccccgt tttgcaggga gtagttccaa tagggtgatt aaggtgctac agcgtgtttg
    54481 ccttcccaga caacttcgcg gtagtgcttc acgtcggtgt cgccttcagt gctgataacc
    54541 agcactacgg catctttgtc cagcgccagt ttttccatca ggctttgatg ttgcgggtga
    54601 taatgaaccg ctgcgagaac gcccaaaccg acagcgccgg attcaccgga gatgatgcgc
    54661 gggtcgttgc cgtacggatt acccagcacg cgcataccta atgcggcaac gctgtcctgg
    54721 caggagatga attgggtggc gcagttacgt aggatttccc agcccagcgg gttaggttcg
    54781 ccacaggcca ggcccgccat gatggtggcc atatcaccgc caacgttgac gatgtcgcct
    54841 ttgacgccgg agcgataaat acagtcagct ttgtcaggtt caacaataat gctgtgcaga
    54901 ttttgcgggc tgtagacgtc gaccagataa cccagcacac caccggccat tgctccgaca
    54961 ccggcttgca gcagaacgtg cgtcggggtt acgcccattt cacgcatttg ctcgacggct
    55021 tcatctgcca gggttgcgta gccttgcatg atccaggttg ggattttggt gtaaccttcc
    55081 catgccgtgt cctgtaccac ttcccagccg tgctgctgcg cgtgttgcat ggtcaggcga
    55141 acggtatcgt catagttcat atccgtgacg atgcactcgg caccgaggtt cagaatggcg
    55201 tcaacgcgtt cctgagcaga acctttcggc atgtaaatca ccgcattctg tccgagttgc
    55261 tgtgctgccc acgccacacc gcgcccgtgg ttgccgtcgg tggtggtcgc gaaagtcatt
    55321 ttttcgccga tggcattttt caggtgctca aatgacagcg tttcgatatc aagatgatat
    55381 ttttcacaca ataattgagc gatggcgtac gcaccgccaa gcattttgaa ggcgttcaga
    55441 ccgaatcgtt ttgattcgtc tttgacgaga attttcttca caccaaaaag gtttgcgaga
    55501 tcgtccagcg cacaaagcgg tgttgggcga taaccagcta ttttctggtg gaactggcgc
    55561 gccagtttgg cctggctttg cgaaaagagc ggtgatgttt cgccgttgaa aaatttgtta
    55621 tcggcgatat caatcttcaa tgagaaaacg gacatatcct atccttttgg cctctggaaa
    55681 tagagggccg accagcagat cggcccgagg ggttatttca cgcgttcttg cgcttctttc
    55741 agcagttgct cgagcagtgc acctggtttg gcgtatttac gggacaggat catcgcggcg
    55801 atgatataag gcttccagct ggcttctttg taggtagcga tacggtattt ttcgaatacg
    55861 ccttcagtca cttcaccttc tttgcaggat acgccgctga tatcagctgg caggcagtgc
    55921 atgtacaggg cttcgccatc acgggtcagt tccatcatct cttcagtaca atgccagtct
    55981 ttgtgttgcg cgttctgtgc cagacactgt ttttccagtg cttttaagcc ttcgtggtcg
    56041 ttcgcacgca gcaattcagt acgctcttcc atcactttgt aaggtgccca tgacttcgga
    56101 taaacgatgt ctgcgtcttt gaaggcttct tccatgctgg tgacctgacg gaagctacca
    56161 ccggaggctt tagcattgtt tttcgccact tcaaccacat ccgggatcag gtcgtagcct
    56221 tccggatggg ccaaggtgac atccataccg aagcgagtca tcagaccgat gatgccttgt
    56281 ggtacagaga gcggtttgcc atagcttgga gagtaggccc aggtcatggc gattttttta
    56341 cctttcaggt tttccagtga accaaagtgt tcacgtaacc acgcgaggtc agccattgac
    56401 tgagtcgggt ggtcaatatc gcattgcagg ttcactaaag ccggacgctg tggcagtaca
    56461 ccctgcttgt aaccgtcatc aagtgcagcg ccaacttcac gcatataggc gttgcctgcg
    56521 cccagataca tatcgtcgcg aataccaata gcgtcggcgc agaaggagat catattggcg
    56581 gtttcacgca cggtttcgcc gtgagcgatt tgtgatttgc cttcgtcgag atcttgttgt
    56641 gcaaggccga gcaggttaag cgcggaagca taagagaagc gggtacgggt ggagttgtcg
    56701 cggaatacgg aaatacctaa tccactatta aagactttgg ttgagatgtt ttcagcacgc
    56761 agtgctttta atgctgcggc aacgtccagt acttgtttca gttcatctgg cgtctgttcc
    56821 cacgttaaca aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat atccttaatc
    56881 agctcattaa cagttttcat catacagtcc cggtagtgga atgacaggca aagtggttta
    56941 tttcactgtg acttgccaaa gtgattggtg ttttcatagc tatacctggg actaacaata
    57001 agtgtgccag aattatccgc gcggctgcaa aagactaatt tgacggataa tgtgtggggt
    57061 tgttcacaaa ttgatgccag cttctggttg tttgtaaaat acgaaaacgt ttggcttttt
    57121 ttatcgcgtt atttatttgc tgctaagtat cagaaataag ataaaggggc aggctatctc
    57181 tcttcagttt gataaactca ccgccgtaat tctcatattg atatagaaat gtgatatctc
    57241 ttccataatt gcattagaaa atgataaaaa tcttatgttt ttatcaaagc caatttcgcc
    57301 tgagttaata tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta
    57361 agtctggaag gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa
    57421 ccgacaattc agcgttttgc cagaatgctt gccagcgttt tgcagtttga ggttgagatc
    57481 gttgatgaaa acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc
    57541 cagttgagcg gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt
    57601 gtgacacagt ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgc
    57661 gaaaaagcat ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt
    57721 ttgattgccg ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct
    57781 gattatgttc gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca
    57841 ggagataaca tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta
    57901 gttgttgatg atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt
    57961 gttgtacaaa ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt
    58021 aattttactc atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc
    58081 attggtcaat tgcataacat tcagggccga caattatttt taatggcatt tcaccaatcg
    58141 cataccagtt tttctgtagc aaatgcacct gatgaaccac atattgaaca attggttggc
    58201 gagtgccgtg ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc
    58261 agcgttatgg tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg agcaatccat
    58321 aagttgagcg gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag
    58381 cagcttctgg aaagcgaact gttcggttat gttaaaggcg catttactag tgcttctgcc
    58441 aacggtaaaa cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata
    58501 ggtgatatgc cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt
    58561 ctgccgattg gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaat
    58621 cagaatttgg cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt
    58681 aatgttatcc cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactattg
    58741 gtgcattact ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct
    58801 cccgatgtcg tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc
    58861 aatttgatgg aatatctggt taacgtggtt ccttcaggtg aagttatcga cagcacgcta
    58921 ttgccgccaa atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcagt
    58981 gaggcgcacc tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa
    59041 atgatccgcg aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc
    59101 atcggcattg ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct
    59161 cagtgaatcc ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag
    59221 aactgtattt acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg
    59281 acgaatttcc gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc
    59341 cataatcagg ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg
    59401 ctgaacatga gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcctttcc
    59461 ttcagcccag gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccaacaccgt
    59521 acaggcacca cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg
    59581 gagtaattcc gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat
    59641 ggtgcattcg atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg
    59701 acggcttcgc taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta
    59761 ctggcccgcc atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttcc
    59821 agcgtttgca ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc
    59881 ggcgttggcg cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc gttatccagt
    59941 cggcaatgtg cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta
    60001 aaatgcgcgc tgcccgcgtg ttctttcggc tgtggcggaa aatgaaaggc gacgaggatt
    60061 tcgtcatgct caagagacac tttgcccggc ccggtgtgaa agccattaat cgggacgaaa
    60121 cgaacaccgc gcggggagtg gatctccagt ttcgcgtcat aaattagcgt tggcgtggca
    60181 gaatctgcgc tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc
    60241 tgcggtccag caatggacgt ggccgcagca cataacgccg ggagatgacg ttgagttata
    60301 ggagcttcta ttagctgggt aaatgtcgtt gcagagccga tacgtagcga gccatcttcc
    60361 gccagcgtaa ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg
    60421 tcattgtgat ggtggagctg aatcagtacg tcagtgccat cggcgagcag tttggcctgc
    60481 gggttgtcag ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca
    60541 aaatcaaaca tgatgttatc ctcaaatcaa tcctgccaga tggaactctt catataaccg
    60601 ttttggcgtc agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg
    60661 aatagcagcg gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga
    60721 ttgcggctca ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg
    60781 cattttgtaa tccagcagat tggggttacg gaccacgccg cttttagcat cgatgatcat
    60841 ctcttcaaat agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc
    60901 cagcagtgga ttaagaatat gccctgaatc atgaacgttg aggatgcggt tgatggtgac
    60961 tttgcacagc gcaatatcga ccgtcagatc aacaaaggta cagccaaacg ccgatgggtt
    61021 agtggtggtt ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa
    61081 agcgtccatc gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac
    61141 gatatggcct tttatcaggg tcagattcat cgctgactga tgtagcatga ctgcggcgtg
    61201 agcgatgatt ttctctttta ataatagtgc cgcactgcgc agcgcaggcg cggcaacata
    61261 gctctggcgt gaggcaaatg cgccgggagc gaacggcgta acgtcggtat cttgagttga
    61321 aataacgcga acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac
    61381 ggtgtcggca ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg
    61441 attcatcaga aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt tagaggtata
    61501 gctaaaacag gcgacgccaa cgccgcggcg caaattgcct tgctggttct ggcattctgc
    61561 acggcgtttt tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc
    61621 gctgtaaata cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa
    61681 acgaatttca acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac
    61741 ggcaaatacg acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt
    61801 atagcaggtc tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt
    61861 cctccccgca gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac
    61921 atccagacta taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatggt
    61981 aaaagcgtgg cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac
    62041 cggaatgccg ccaagcttgc tggtcaggaa tgctgccatt ggctcttcca gtacatcctg
    62101 tttattacca aaaccgccac cgacaaacgg tttgatgact cgtacgcatg accagggaat
    62161 atccagcgcc tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat
    62221 ggtaattcgc gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg
    62281 ttgaataacg ggggtctgat agtgcccctg tacctggtag tcggcggcat cgattgtttg
    62341 ttggacatta cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg
    62401 tgcagcgtct tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc
    62461 aatgctgacc aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc
    62521 aacggcgtca ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg
    62581 cttgttttcg tcaagtgtcc aggcatgccc agctgtagcg aatggaatat caggcacatc
    62641 ttcccaggta aaaatcgcca gtacgcccgg caaacttctg gcttgttcat cattaatact
    62701 tacggcataa ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat
    62761 aacataatcg tcagtatatc gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat
    62821 gcatgattca cccgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac
    62881 atttggttct tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga
    62941 taagcgttag gggaggtagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa
    63001 caggactctg aataggggca gtgatacgta tcaatatgag tggcgataaa atcaatgtga
    63061 taaattggga ttattacgca tcaggatggg aaatagggta aaaagtcagg aagttcgata
    63121 cctctacagc gaatattttg ctgatgatac ttccctttgc caaatgaaat taacctcttt
    63181 ctctattacc acgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga
    63241 taaggcgtct tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa
    63301 tctctgggta tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc
    63361 aaatcatccg atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg
    63421 ctatatcgta tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg actgaacggc
    63481 atttcccccc cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt
    63541 agcagtagta cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg
    63601 tcatcagcgg taccgaaatc atcctggccg ccagtagggc aacgttgttg gttatggcca
    63661 acgacaggga aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat
    63721 atctcagctc cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg
    63781 ggcaaccagc tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt
    63841 acggcttacg cccataatga cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg
    63901 caaaaaatcg ccactatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt
    63961 cgttaccgtg caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca
    64021 aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa
    64081 ggtttataat gccttacgca tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc
    64141 ccaagctcta tacgagggtt cgattccctt cgccgctttt gctgtatctg attttctata
    64201 ttatcttaat tcaatcgctt cagacttttc gcaatcgccg taatatttcc ccattttgtt
    64261 gtaaataaaa agcgtttacg caatgcaatc cttttgtaac aacccaggta aaacacctta
    64321 ttttcaagtt gttaaataag tggttattta cgcttttaac aaacgattga tggaatattt
    64381 tatgaaaggg aaatctgcat taactcttct gctcgctggt atattttcgt gcggcacatg
    64441 ccaggcgaca ggggcagaag tgacgagtga atctgtattt aacattctta attccacggg
    64501 ggcagcaaca gataaaagtt atttatcgtt aaatccagat aagtacccta attatcgtct
    64561 tctgattcac tcagccaaat taaaaaatga aataaagagc cactatacca aagacgaaat
    64621 tcaagggttg ttaacattaa cagaaaacac acgaaagtta acgttgacgg agaaaccctg
    64681 gggaacattt attcttgctt ctactttcga agatgacaaa acagcggcag aaacccacta
    64741 tgatgctgtc tggctgaggg atagtttatg gggctatatg gcattagtat cagatcaggg
    64801 aaatagtgta gccgcaaaaa aggttctgct caccctgtgg gattatatgt ctacgctcga
    64861 ccagattaag cggatgcagg acgtcattag caaccccaaa cgactggatg gagttcccgg
    64921 gcaaatgaat gctgtgcata tccgatttga cagcaactcc cccgtgatgg cagatgtgca
    64981 ggaagaaggc aagccgcagc tgtggaacca taaacaaaat gatgcgttag gtctttatct
    65041 tgatctcctt atacaagcaa tcgacactgg tacaattaac gctgaagatt ggcaaaaagg
    65101 tgaccgatta aaatcggttg cgctgctgat tgcttatctg gataaagcca atttctatgt
    65161 tatggaagat tccggagcat gggaagaaga tgcccgatta aacacatctt ccgttgcact
    65221 ggtgacctct ggactggaac gtttgtcgaa cttactctcg aagaaagact ctgtttttgt
    65281 ctctgattta ctacgcgaag ctaaagccaa tgaactggat gaacctcttt caacaacgag
    65341 gttgaaccac ctcatagata aaggttatga aagaataacg ttacagcttg atttaggggg
    65401 agaatcccca ggttatctgg aaaaagataa acattacaga gaagccgatg cggctttact
    65461 taatgttatt tatcctgcaa atcttgcaaa gataaacacc agaagaaaag agcaggttct
    65521 aaaaatagtt aaaaaactag caggtccgta tggtataaaa agatacgaga aggataatta
    65581 tcagtcggca aatttttggt tcaatgatat taaaaccgat accgatcaaa attcacatgc
    65641 gaaaag
//
LOCUS       DAQSWQ010000009        64811 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.009, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000009 DAQSWQ010000000
VERSION     DAQSWQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.009"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="R8G18_000600"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="R8G18_000600"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEE9794391.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="R8G18_000601"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="R8G18_000601"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HEE9794392.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="R8G18_000602"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="R8G18_000602"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEE9794393.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="R8G18_000603"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="R8G18_000603"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HEE9794394.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="R8G18_000604"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="R8G18_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HEE9794395.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /locus_tag="R8G18_000605"
     CDS             complement(4809..7043)
                     /locus_tag="R8G18_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEE9794396.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="R8G18_000606"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="R8G18_000606"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEE9794397.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="R8G18_000607"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="R8G18_000607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HEE9794398.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="R8G18_000608"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="R8G18_000608"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HEE9794399.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="R8G18_000609"
     CDS             complement(12798..14138)
                     /locus_tag="R8G18_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HEE9794400.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="R8G18_000610"
     CDS             complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="R8G18_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HEE9794401.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="R8G18_000611"
     CDS             complement(15927..16376)
                     /locus_tag="R8G18_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEE9794402.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="R8G18_000612"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="R8G18_000612"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HEE9794403.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="R8G18_000613"
     CDS             complement(17176..17505)
                     /locus_tag="R8G18_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HEE9794404.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="R8G18_000614"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="R8G18_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HEE9794405.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="R8G18_000615"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="R8G18_000615"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEE9794406.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="R8G18_000616"
                     /gene_synonym="ygdH"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="R8G18_000616"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HEE9794407.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21620..22909
                     /gene="sdaC"
                     /locus_tag="R8G18_000617"
     CDS             21620..22909
                     /gene="sdaC"
                     /locus_tag="R8G18_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HEE9794408.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22967..24334
                     /gene="sdaB"
                     /locus_tag="R8G18_000618"
     CDS             22967..24334
                     /gene="sdaB"
                     /locus_tag="R8G18_000618"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HEE9794409.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALHRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24446..25201
                     /gene="xni"
                     /locus_tag="R8G18_000619"
     CDS             24446..25201
                     /gene="xni"
                     /locus_tag="R8G18_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HEE9794410.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="R8G18_000620"
     CDS             complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="R8G18_000620"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEE9794411.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="R8G18_000621"
     CDS             complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="R8G18_000621"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEE9794412.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27676..28992
                     /gene="fucP"
                     /locus_tag="R8G18_000622"
     CDS             27676..28992
                     /gene="fucP"
                     /locus_tag="R8G18_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEE9794413.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29025..30800
                     /gene="fucI"
                     /locus_tag="R8G18_000623"
     CDS             29025..30800
                     /gene="fucI"
                     /locus_tag="R8G18_000623"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEE9794414.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30909..32327
                     /gene="fucK"
                     /locus_tag="R8G18_000624"
     CDS             30909..32327
                     /gene="fucK"
                     /locus_tag="R8G18_000624"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HEE9794415.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLEEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32329..32751
                     /gene="fucU"
                     /locus_tag="R8G18_000625"
     CDS             32329..32751
                     /gene="fucU"
                     /locus_tag="R8G18_000625"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HEE9794416.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32809..33540
                     /gene="fucR"
                     /locus_tag="R8G18_000626"
     CDS             32809..33540
                     /gene="fucR"
                     /locus_tag="R8G18_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HEE9794417.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="R8G18_000627"
     CDS             complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="R8G18_000627"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HEE9794418.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34677..35072)
                     /locus_tag="R8G18_000628"
     CDS             complement(34677..35072)
                     /locus_tag="R8G18_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEE9794419.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="R8G18_000629"
     CDS             complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="R8G18_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HEE9794420.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36359..36586)
                     /locus_tag="R8G18_000630"
     CDS             complement(36359..36586)
                     /locus_tag="R8G18_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HEE9794421.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36778..37983
                     /gene="csdA"
                     /locus_tag="R8G18_000631"
     CDS             36778..37983
                     /gene="csdA"
                     /locus_tag="R8G18_000631"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HEE9794422.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37983..38426
                     /gene="csdE"
                     /locus_tag="R8G18_000632"
     CDS             37983..38426
                     /gene="csdE"
                     /locus_tag="R8G18_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HEE9794423.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="R8G18_000633"
     CDS             complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="R8G18_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HEE9794424.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="R8G18_000634"
     CDS             complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="R8G18_000634"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEE9794425.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40666..40742
                     /locus_tag="R8G18_000635"
     tRNA            40666..40742
                     /locus_tag="R8G18_000635"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40700..40702,aa:Met,seq:cat)
     gene            40776..40852
                     /locus_tag="R8G18_000636"
     tRNA            40776..40852
                     /locus_tag="R8G18_000636"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40810..40812,aa:Met,seq:cat)
     gene            40886..40962
                     /locus_tag="R8G18_000637"
     tRNA            40886..40962
                     /locus_tag="R8G18_000637"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40920..40922,aa:Met,seq:cat)
     gene            complement(41037..42290)
                     /gene="amiC"
                     /locus_tag="R8G18_000638"
     CDS             complement(41037..42290)
                     /gene="amiC"
                     /locus_tag="R8G18_000638"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HEE9794426.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42522..43853
                     /gene="argA"
                     /locus_tag="R8G18_000639"
     CDS             42522..43853
                     /gene="argA"
                     /locus_tag="R8G18_000639"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEE9794427.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43915..45741)
                     /gene="recD"
                     /locus_tag="R8G18_000640"
     CDS             complement(43915..45741)
                     /gene="recD"
                     /locus_tag="R8G18_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEE9794428.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45741..49283)
                     /gene="recB"
                     /locus_tag="R8G18_000641"
     CDS             complement(45741..49283)
                     /gene="recB"
                     /locus_tag="R8G18_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEE9794429.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49276..52164)
                     /gene="ptrA"
                     /locus_tag="R8G18_000642"
     CDS             complement(49276..52164)
                     /gene="ptrA"
                     /locus_tag="R8G18_000642"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HEE9794430.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52340..55708)
                     /gene="recC"
                     /locus_tag="R8G18_000643"
     CDS             complement(52340..55708)
                     /gene="recC"
                     /locus_tag="R8G18_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEE9794431.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55721..56044)
                     /locus_tag="R8G18_000644"
     CDS             complement(55721..56044)
                     /locus_tag="R8G18_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEE9794432.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56029..56436)
                     /locus_tag="R8G18_000645"
     CDS             complement(56029..56436)
                     /locus_tag="R8G18_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HEE9794433.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56433..56996)
                     /locus_tag="R8G18_000646"
     CDS             complement(56433..56996)
                     /locus_tag="R8G18_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HEE9794434.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56987..57457)
                     /locus_tag="R8G18_000647"
                     /pseudo
     CDS             complement(56987..57457)
                     /locus_tag="R8G18_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(57642..58436)
                     /gene="thyA"
                     /locus_tag="R8G18_000648"
     CDS             complement(57642..58436)
                     /gene="thyA"
                     /locus_tag="R8G18_000648"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEE9794435.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58443..59318)
                     /gene="lgt"
                     /locus_tag="R8G18_000649"
     CDS             complement(58443..59318)
                     /gene="lgt"
                     /locus_tag="R8G18_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEE9794436.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59469..61715)
                     /gene="ptsP"
                     /locus_tag="R8G18_000650"
     CDS             complement(59469..61715)
                     /gene="ptsP"
                     /locus_tag="R8G18_000650"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEE9794437.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61728..62258)
                     /gene="rppH"
                     /locus_tag="R8G18_000651"
     CDS             complement(61728..62258)
                     /gene="rppH"
                     /locus_tag="R8G18_000651"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEE9794438.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62943..63632
                     /gene="mutH"
                     /locus_tag="R8G18_000652"
     CDS             62943..63632
                     /gene="mutH"
                     /locus_tag="R8G18_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HEE9794439.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63701..64414
                     /locus_tag="R8G18_000653"
     CDS             63701..64414
                     /locus_tag="R8G18_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEE9794440.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64552..64770
                     /gene="ygdR"
                     /locus_tag="R8G18_000654"
     CDS             64552..64770
                     /gene="ygdR"
                     /locus_tag="R8G18_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HEE9794441.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15711 a        17424 c        15889 g        15787 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ctggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttag
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaggcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaagcgc tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccat acggatcttc
    12001 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgctaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12301 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgta aagaacggtg cgtgtgcccc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacagacgtc gtaaattgag aactcatatt
    12781 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    13141 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata
    13321 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca
    13441 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13741 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    13981 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgataag gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17041 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgtc tgcaactgga aacgaacgcg gtcatgagtg gtcataaagc aacttcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17581 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18961 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca cctgtggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgcgttgca
    19981 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21121 atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga taaatatcat
    21181 tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat tttcataaac
    21241 cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    21301 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc agatgtcgtt taatgggcaa
    21361 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    21421 tcttccaagt agattattgt atttgggatc aagatcactg atagatacat aacttgtgtg
    21481 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    21541 tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg aattatttgc
    21601 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    21661 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    21721 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    21781 catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc tgactcgctt
    21841 cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag aacactttgg
    21901 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    21961 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22021 gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    22081 tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc cgtttgttgg
    22141 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    22201 gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc
    22261 aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt tcgccgttgc
    22321 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc tggcattcgc
    22381 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac tgagcctgac
    22441 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    22501 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    22561 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    22621 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    22681 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    22741 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    22801 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    22861 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    22921 ccgctttcgg gcggcgcttc ctccgtttta acgcgatgta tttcctatga ttagcgtatt
    22981 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    23041 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    23101 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    23161 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    23221 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    23281 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    23341 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    23401 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    23461 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    23521 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    23581 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    23641 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    23701 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    23761 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    23821 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agttcatccg
    23881 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    23941 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24001 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    24061 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    24121 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    24181 cgccgcacgt atggcgctac accgtaccag cgagccgcgc gtctgcctcg ataaagttat
    24241 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    24301 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    24361 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    24421 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    24481 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gccagcatgc
    24541 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    24601 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    24661 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    24721 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    24781 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    24841 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    24901 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    24961 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25021 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    25081 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    25141 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    25201 acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt atataccgga
    25261 taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc aggcggtatg
    25321 gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc
    25381 atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa
    25441 atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg
    25501 cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta
    25561 cttctcaccg gtaaagtcag cgttatagcg catgacatgc ggtaacagga tggcgttcgc
    25621 aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat gcaccaaccc
    25681 taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc
    25741 ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt
    25801 aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg
    25861 agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc
    25921 agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg
    25981 tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac ctgctgtggt
    26041 aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt ccaggctacg
    26101 cacatcggca aactccgggt tgttgctgat aatgccaatc gctttacaag tatcctgtgg
    26161 agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc
    26221 ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag
    26281 ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt
    26341 atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc
    26401 aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct
    26461 tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc
    26521 cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt
    26581 cgtcacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag
    26641 attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag
    26701 agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg
    26761 aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag
    26821 aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct
    26881 gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc cttcctcatg
    26941 tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc
    27001 tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg
    27061 gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt
    27121 tcgttccatt agctatctct ctctgattca aaacagggca ataatgttgt tcctttcaca
    27181 ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc
    27241 acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc aagttctctt
    27301 tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tcatttttct
    27361 attcacccaa gccaggaatg cccgttttcg gcataaacaa ttaatacagt catctggttt
    27421 gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta aagtgatggt
    27481 agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta cagagcgttt
    27541 tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac tgatatgaag
    27601 cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt aagcttctga
    27661 acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg tgcggtagat
    27721 aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg ctcactgttt
    27781 tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt ccagcaggct
    27841 tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt tggttatttc
    27901 attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc aggaattatt
    27961 actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc agaaataatg
    28021 aactacacct tatttttagt tggcctattt attattgcag ccggattagg ttgtctggaa
    28081 actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca cttccgctta
    28141 aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt
    28201 cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat gtctccagag
    28261 caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta tatgatcatc
    28321 gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc ggcattgcag
    28381 agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc tcgcctagcg
    28441 cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg cgcacaaacg
    28501 gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttctaggtat gactgcaggc
    28561 tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg tttcaccggt
    28621 acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc attaatcgct
    28681 atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat agccctgact
    28741 ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg cattaagaat
    28801 ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat tggcggcggt
    28861 attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa
    28921 ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg ctctcaaacg
    28981 gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct
    29041 taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc
    29101 ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc gagaaactgc
    29161 gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg
    29221 ctgaagccgc tgcttgcgaa gaaaaattca gcagccagaa tgtaggcctc accattacgg
    29281 taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag
    29341 ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca gcggctctgg
    29401 cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac gttcaggatg
    29461 ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg
    29521 gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg
    29581 gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga atgaaagtcc
    29641 aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg
    29701 aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa gatgaaaata
    29761 acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga
    29821 tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat attggtcgcg
    29881 tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga
    29941 ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca tttgactgga
    30001 atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa
    30061 tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg cgtacctact
    30121 ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg gcagaacacg
    30181 gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg
    30241 acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa gaggctgacg
    30301 cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt ggcggcggtt
    30361 actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt gtcaacatca
    30421 tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg
    30481 atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc tggtttgcac
    30541 cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg aactggggcg
    30601 ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact ctcgcctcca
    30661 tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat cgtccttctg
    30721 cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc cagaactacg
    30781 gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg ctccggctac
    30841 cgggcctgaa caagcaagag tggttagccg gataagcaat gttatccggc tatattgcag
    30901 gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc aatgtcaggg
    30961 ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg
    31021 atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt ttgcaacgct
    31081 ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc cgcggtatcg
    31141 ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat ctgctctatc
    31201 cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt gaacagttga
    31261 tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc aatacgttat
    31321 ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc
    31381 tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact gatatcacga
    31441 tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg caaattttac
    31501 aagccaccgg tattccacgc cgactcttcc ctcgtctgga ggaagcgggt gaacagattg
    31561 gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata ccggtgattt
    31621 ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa aatgaacccg
    31681 tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt gatacttcgc
    31741 tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg ttgtataacc
    31801 caggtatgca atggctggca tccggcgtgc tggaatgggt gagaaaactg ttctggacgg
    31861 ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct ggcgcggatg
    31921 gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa ggagtgacgc
    31981 ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact gcgcaattac
    32041 agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa ttattgttag
    32101 tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg cttgatattc
    32161 cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg ttcggttggt
    32221 atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat tatcagtacc
    32281 gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat gctgaaaaca
    32341 atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg acatggagat
    32401 gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca ggtgatccgc
    32461 gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt tgagctggac
    32521 agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct cgatcctgaa
    32581 gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga catcatccgc
    32641 atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt tatcacaggc
    32701 gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta atctcatatc
    32761 ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat gaaagcggca
    32821 cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac ggaagctctc
    32881 tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga attacagacg
    32941 cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca aaatcaagac
    33001 agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc agatatcgcg
    33061 cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc cagttcaact
    33121 tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac caatagccat
    33181 ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc cggcggcaca
    33241 cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact taaatcgctg
    33301 gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc actgtgggac
    33361 tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc gcaatcgttg
    33421 ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg gcatctggat
    33481 gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt aacagcctga
    33541 cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc tcgtcgcgac
    33601 gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg cgatcgtgat
    33661 acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt gcctgaatat
    33721 acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg agattgaaaa
    33781 tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca actttagccg
    33841 gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga cggaatttga
    33901 aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc gccatcgggc
    33961 cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc cagccgccag
    34021 ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc cactcatccg
    34081 caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc ggcgcctctg
    34141 ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg ctgtatgagt
    34201 aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga cgcttcggcg
    34261 tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc acggtgaatt
    34321 tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca acacgcagtt
    34381 caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata cgatcttctg
    34441 gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa attaatgaac
    34501 tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat tcataaatga
    34561 cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg ccggctttat
    34621 cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat acaaccttat
    34681 tcatgactta cgcccttgca ctttaaacgg atagcaccaa ctaacattaa cgcccagccc
    34741 gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag atgggacagc
    34801 gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa aacgctactc
    34861 cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa gatcgccagc
    34921 gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac ggcccccatg
    34981 gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa gccgctaatg
    35041 gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa ttattgttca
    35101 taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca ttggcgaaag
    35161 gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa agcgttttta
    35221 ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcaatttc agattgcgcc
    35281 atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag caccatggcg
    35341 ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa ctgtcgggta
    35401 tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc cagatcttcc
    35461 ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg caataaatat
    35521 tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata aaatatcgcc
    35581 acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat tcgaacgtca
    35641 attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg aatggcgaaa
    35701 ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag tttacgcgtc
    35761 gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg cccttcctcg
    35821 gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa atcctcaaga
    35881 gacttgattt gatgacttac tgcggcctgg gtcacgaaaa gctcttctgc tgcgcgagtg
    35941 aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg tggtaatcgt
    36001 ttagacatgg cttttaaact ttgatgttaa attaatttaa caattagatc acactgtgta
    36061 acctattagt ttttttaatc tgagccatta taaattgtcc gttgagcttc taccagcaaa
    36121 tacctatagt ggcggcactt cctgagccgg aacgaaaagt tttatcggaa tgcgtgttct
    36181 ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt gtttgcaatt ggtctgcgat
    36241 tcagaccatg gtagcaaagc tacctttttt cacttcctgt acatttaccc tgtctgtcca
    36301 tagtgattaa tgtagcaccg cctaattgcg gtgctttttt ttaccttgcg atcgcgaatt
    36361 actgatccag ttcgaccatc tctttcacgt cagtacggtt gatctgctgt ttgttgccat
    36421 tagcgtcttt atacgaaatc ataccggtat cgttatcagt ctgtggtttg ccgtcagaga
    36481 cgatggtacg tccgtcattg gtgtgcatca catagttcga accggaacag gcgctcaggg
    36541 caaaagtcag catacaggca gaaataattg cggcagtctt tttcatcatc ttctccttaa
    36601 agcgattaat aaatcttaat agtattgatt aacaggctaa aattaacgcc taacactatt
    36661 cagcatatgt tacttacgcg gcttcgccag gatatccaga taattctgat ggttagcact
    36721 ctccttgtat caaagtgaat tttgcgtcac gatcggtgca tcaagccgag gagtaccatg
    36781 aacgttttta atcccgcgca gtttcgcgcc cagtttcccg cactacagga tgcgggcgtc
    36841 tatctcgata gcgccgcgac cgcgcttaaa cctgaagccg tggttgaagc cactcgacag
    36901 ttttacagcc tgagcgccgg aaacgtccat cgcagccagt ttgccgaagc ccaacgcctg
    36961 accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat tactgaatgc accggatgat
    37021 aaaactatcg tctggacgcg cggcaccact gaatccatca acatggtggc acaatgctat
    37081 gcgcgtccgc gtctgcaacc gggcgatgag attattgtca gcgtggcaga acaccacgct
    37141 aacctcgtcc cctggctgat ggtcgcccaa caaactggag ccaaagtggt gaaattgccg
    37201 cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag aactgattac tccccgtagt
    37261 cggattctgg cgttgggtca gatgtcgaac gttactggcg gttgcccgga tctggcgcga
    37321 gcgattacct ttgctcattc agccgggatg gtggtgatgg ttgatggtgc tcagggggcg
    37381 gtgcatttcc ccgcagatgt tcagcaacta gatatcgatt tctatgcttt ctctggtcac
    37441 aaactgtatg gcccgacggg tatcggcgtg ctgtatggca aatcagaact gctggaagcg
    37501 atgtcgccct ggctgggtgg cggcaaaatg attcacgaag tgagttttga cggcttcacg
    37561 actcaatctg cgccgtggaa actggaagca ggaacgccaa atgtggctgg tgtcatagga
    37621 ttaagcgcgg cgctggaatg gctggcagat tacgatatca accaggcaga aagttggagt
    37681 cgcagcctgg cgacgctggc ggaagatgcg ctggcaaaac gtcctggctt ccgctcattc
    37741 cgctgccagg attccagcct gctggccttt gattttgctg gcgttcatca cagcgatatg
    37801 gtgacgctgc tggcggagta cggtattgcc ctgcgggccg ggcaacattg cgctcagccg
    37861 ctactggcag aattaggcgt aaccggcaca ctgcgcgcct cttttgcgcc atataataca
    37921 aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg cgctggaatt attggtggat
    37981 taatgacgaa cccgcaattc gccggacatc cgttcggcac aaccgtaacc gcagaaacgt
    38041 tacgcaatac cttcgcaccg ttgtcgcaat gggaagataa atatcgccag ttgatcatgc
    38101 tggggaaaca gcttccggct ttgccagacg agttaaaagc gcaggctaaa gagattgccg
    38161 gatgcgaaaa ccgcgtctgg ctgggatata ccgtgactga aaacggtaaa atgcacttct
    38221 ttggcgatag cgaagggcgc attgtgcgcg gcctgctggc ggtgctgttg actgccgttg
    38281 aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct ggcattgttt gatgagctgg
    38341 gattgcgtgc gcaacttagc gcctcacgca gccaggggtt aaatgcgtta agcgaggcga
    38401 ttatcgctgc gacgaagcag gtttaagcat agaaccgggt cggataagac gttcgcgtct
    38461 catccgacct gattgtttaa ccctgacgcg ccgctttcgc catcatcttc ttcagcgcat
    38521 gagaaaccgc aacaaaccca aaggtggcgg tcaccatcgt tgccgcgcca aatccagatg
    38581 cacaatccat ccgcttcggc ccttctgccg tggctttcat cgcacacaca gtaccgtctg
    38641 actgcgggta caccagcgct tcagtagaaa acacgcaatc gacgccgagc ttacctttac
    38701 tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg caacttcgcc gccagcggat
    38761 cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt cgggtcaatc tgcccacccg
    38821 cgccaccggt cgtgaccagc gggattttat tgcgccgaca ataagcaatc agcgccgctt
    38881 tgggccgtac actatcaatg gcatcaatca cgtacgaata acccacgctc atatactgcg
    38941 ctacgttatc cggcgtcacg aaatcatcca ccaccgttac acggcactcc gggttaatct
    39001 ggcgaatacg ctccgccatg acttccgctt ttgccagccc gacgttatcg cgcagagcgt
    39061 gaatttgccg attggtattg gtgacgcaca catcatccat atcgataagc gtgattgcgc
    39121 caatccccgt gcgcgccagc gcttccgccg cccaggaacc gacaccaccg ataccaacca
    39181 cacaaatatg cgcgtcagca aacagttgca acgctttttc accatacaga cgcgctgtgc
    39241 caccaaaacg ctgacgccat gcatcactaa ttaccacaga catagcacct cagaattaaa
    39301 aagggtgagg ttatccctca ccctgtcgta tccgtaaaaa cggtatacag aataacacat
    39361 cagccgctaa agacgttacc tgcgcccggg gcggttttca gcacccagac acgtccatag
    39421 tggttgtacc aacctgcgcg gtgtccggct tccggcccga tcccttgata gatatcgaag
    39481 tgttggcctt tgattgcacc accgacatcc agcgccacca tcagacgcag ttcgtactga
    39541 ccattaaatt tgccgttatt atccagcaac ggcacttctg ccagcaaggt agtacctggc
    39601 ggaataatgg aacgatcaga ggcaactgac gcgcgaccaa ccagcggcac cgcacttgcc
    39661 cctttcaccg gtgcaaaaga ttgcggttta aagaagacga aagacgggtt ctgttccagc
    39721 agctcgcgaa cctcggcttc actgtgtgtt tcgccccagt gacgaatcgc ctgcatcgac
    39781 atatcttctt ttttcacttc gccacggtcg atcagcacct taccaatgct gcgataggca
    39841 tgaccgtttt tccctgcata gctgaaaaag ttaagcggac tgccatcacc aaagtcgata
    39901 tacccactac cctgcacatc cataatgaag ttatccatca gggagttact gtaagcgaga
    39961 atatatttat cactcaatgc ccccgcgtag atctccgcac gagacggcag acgaccacgt
    40021 tttggcggca tacggtaaat aggatactgg aactcgccct ggcgggtatg gcgcgcctga
    40081 attaccggcg tgtaatagcc tgtaaactgc acgttaccat agttgtcggc accttccatc
    40141 tgccaggcat caatgccgaa ctggcgcata ttgcgggtat caccgcctgc gcgcagccac
    40201 tcttgcaccg cgttataaac attactctgg ttgccataca gacgcggtga cgaattacgg
    40261 atatggttaa tttgctcggc aaaatcaccg gcgttaatcg gcgcgccaac ggcatctggc
    40321 tggttcacca gagagaaagg ctgggtaaat ttcccgtctt tatattgctg tccgcgatcg
    40381 gttggtttgg aagagcaggc ggcaagcatt gccacaaccg tgcccataag aaggtacttt
    40441 acccaacgtc ctttcattgt tctcttcttc aggttaaaaa ataaggcgca acgaagataa
    40501 caaaccgccg cgtttaatga aatgagcagc ctctccctga cgcaaatttt gcacaaaaaa
    40561 taggctttag tgatttgttt ttgttcaaaa tcatgccaga tccgtgatcg gggtaaaaaa
    40621 aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc acggaggcgg ggtggagcag
    40681 cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac
    40741 caattaaaat ttgatgaagt aaagcagtac ggtgacgcgg ggtggagcag cctggtagct
    40801 cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caatcaaatt
    40861 tgatgaagta aaagcaatac ggtgacgcgg ggtggagcag cctggtagct cgtcgggctc
    40921 ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caattattga acaccctaac
    40981 gggtgttttt ttgtttctac tctcccataa aaaaagcgcc attcagcgcc tttttatcat
    41041 ccccttctcg ccagcgttgc cccatcggca aaatacgctt taatccccgc aagaatagac
    41101 tccgcaactt cctgctggaa agtcgccgtt ttcagtttac gctcttcctc aacgttactg
    41161 ataaacgccg tttcgaccag aatggaggga atatctggtg cctttagtac ggcaaacccg
    41221 gcctgttcaa cttgattttt atgcagcttg ttgattttac cgagcttatt cagcactgct
    41281 ttaccaaact taaggctgtc agcaatggtc agcgactgca ccatatcgaa catggtgtgg
    41341 tcgacatagc ggtcaccgct tttgctcacg ccaccaatca agtccgaggc gttctgggtt
    41401 tgtgccagat attttgccgc agtactggtt gcacctttgg ttgagagcgc aaacaccgag
    41461 gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga tagagacaaa caggtcagca
    41521 cgctgtttct gggcttttgc tacgcgcact tgcaacggaa tgaagatgtc ttcattgcgc
    41581 gtcatgtaca ccttcatatt gccctctttc tcgatcagag agcgcaaacg gcgagctatt
    41641 tgcaatacca catctttttc gcgcgttttg tatttcccca ccgcaccgga gtcttcgcca
    41701 ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc gccctgcttt acctggttgt
    41761 ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc ctttgttgta atcctccagc
    41821 agcgccagca gcgggtcctg catatcctgt gcattggccg gatagaggtc catcaccaga
    41881 cgctctttaa acccggcgac cggcgcaagg gcaaacagct gcggttttac gttttgcttt
    41941 aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc cgacgcgcgc cgacttgatg
    42001 aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga gtaccgagtt caggtttaca
    42061 tcttcgatat ccaccaccac gcgttcaggg ttactcaacg cgaactgctt atatttcagc
    42121 tgacgatttg attctaccgt cacgcgggtg tagctggacg caggccagac gcgcaccgcc
    42181 acgacctggc taaccgcagc caggctgacc tgacttacgc tcaatagcca catagcgccc
    42241 gcgccttgca gtaaacgacg acggctgatt gcagtgttgg atcctgacat gcctctcccg
    42301 agcaaaagaa atctaagctg tgtaacaagt aaacgactaa tttgaccgga aactttagcg
    42361 aaagacgcat aatctgtcat ctaataaacg gtaaacattc tttttatatt cacggcatta
    42421 ctgataaaaa agtcgctctc gcataaaatt tacacttgca ccctgcgaaa aaacagaata
    42481 aaaatacaat aatttcgaat aatcatgcaa aggggtgtgc cgtggtaaag gaacgtaaaa
    42541 ccgagctggt cgagggattc cgccattcgg ttccctatat caatacccac cggggaaaaa
    42601 cgtttgtcat catgctcggc ggtgaagcca ttgagcatga gaatttctcc agtatcgtta
    42661 atgatatcgg gttgttgcac agcctcggca tccgtctggt ggtggtctat ggcgcacgtc
    42721 cgcagatcga cgcaaatctg gctgcgcatc accacgaacc gctgtatcac aagaatatac
    42781 gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc tgcgggaaca ttgcaactgg
    42841 atattactgc tcgcctgtcg atgagtctca ataacacgcc gctgcagggc gcgcatatca
    42901 acgtcgtcag tggcaatttt attattgccc agccgctggg cgtcgatgac ggcgtggatt
    42961 actgccatag cgggcgtatc cggcggattg atgaagacgc gatccgtcgt caactggaca
    43021 gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt cactggcgag agctttaatc
    43081 tgacctcgga agagattgcc actcaactgg ccatcaaact gaaagctgag aagatgattg
    43141 gtttttgctc ttcacagggc gtcactaatg acgacggtga tattgtctca gaacttttcc
    43201 ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa aggcgattac aactccggta
    43261 cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag cggcgtgcgt cgctgtcatt
    43321 taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt gttctcacgc gacggtatcg
    43381 gtacgcagat tgtgatggaa agcgccgagc agattcgtcg cgcaacaatc aacgatattg
    43441 gcggtattct ggagttgatt cgcccgctgg agcagcaagg tattctggtg cgccgttctc
    43501 gcgagcagct ggagatggaa atcgacaaat tcaccattat tcagctcgat aacacgacta
    43561 ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat tggggaaatg gcctgtgtgg
    43621 cagttcaccc ggattaccgc agttcatcac ggggcgaggt tctgctggaa cgcattgccg
    43681 ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct gaccacgcgc agtattcact
    43741 ggttccagga acgtggattt accccggtgg atattgattt actgcccgag agcaaaaagc
    43801 agttgtacaa ctaccagcgt aaatccaaag tgttgatggc ggatttaggg taagcgatga
    43861 aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca tccggcaatt acgtttatcc
    43921 ccgcgaacta aacaacgccg ccagaccact gcgccgctca gtacgagtgg cgattgccgc
    43981 acttaatata cgctcatcgg catacagcga cagacggcga cgcgcgcggg tcaccgcggt
    44041 gtataccagc tctcgcgtta ctatcggcgt gcgttggctc ggtaaaatca acgccgcatg
    44101 gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc cacgtagttt cgtgctctgg
    44161 caagcgactc ggttgcacag acttaatatt gccgtccggc atcgcaaacc agacgcgcgt
    44221 cccctgcccg cgatccagcg caataccgat atcgccatta aacaacccaa gcgcactgtc
    44281 attacgggca atcatcaccg gtcgaccttc gtaccaacga gagtgcgaat gacgatgaat
    44341 tttgcgcttc tgttgcataa actgctcaat tcgctcattc agtccagcca cgccaaatgg
    44401 cccttcccgc agggcgcaca aaagttggta ctcattgaac gcctgaatga ttaaatccgg
    44461 ctcggcacgc gcttgcagca gatccagata acgtccataa cccgcaagag cttcctcaag
    44521 catcgcaata taatcttcgc cgctctgtaa aagccgtttt tcaatatcag taaaatcctg
    44581 ctgaaaaacg gttttcactg ccgttttatc accacggttg atcgccgcag ctaactgacc
    44641 aatgccagaa tcgctgccga aacgatagct tttttgcagc aggcagagac tgtcgcgcaa
    44701 agatgccgct tctgtgccgg ttcctgccgg aacgtgagtt cccgtcaggc ggcttagctg
    44761 cccggcacgc tcggcggtaa agccggcatt ggcataagcg cagatatcgc ccagcacagc
    44821 cccagcctca accgaggcca gttgatcacg atcgccgaga aagatcactc gcgcatgatc
    44881 gggcaaggcg tcgatcagtc tcgacatcat aggcagatcg atcattgacg cttcatctac
    44941 caccagcaca tcaagatgca gcgggttacc ggcatgatga cgtaaacgct ggctacccgg
    45001 ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc ggaatgcgtt tcttttgttc
    45061 atcggtcagc ggtaactgtc gcaaagcctt gccgagagat tcggttaagc gcgcggcagc
    45121 tttacccgtt ggtgcagcca gacggatacg gcagcgttcg ccgtcggcca tttgaattaa
    45181 cgctgccagc aacttcgcca cggtggtcgt tttaccggtg cctgggccgc cggaaatcac
    45241 cgagatccgc cgcgtcagcg ccactgctgc cgccactttt tgccagttaa tttcatcgct
    45301 tactggaaaa agtttgtcca gggtttgcgc cagtagagct tcatcaacct caatggcatg
    45361 attcacctca ttgaagaagc gcgctaccgt ccgctcgtta caccacatgc gattcaaata
    45421 aagacgatcg ccacagagga tcatcggcgt gggttcatct cccctgctga ccgcttgaga
    45481 agccagcaag cattcttccc aattttgtag ctcaccgatt tcactgacac aggtcgccaa
    45541 cagcggatgc gacgcctcgt tattttccag tcgtgaaagc ggcaaacaga cgtgtccctc
    45601 tccggcatca tgacttaaca gtgccgccgc gagggtgacg gcaggatgtt catctcccgc
    45661 cacggtcagg gcaaattgca catccagcgg gcgtagctgt ttgtgctcca cagcttccag
    45721 taattgcttt tgcaatttca ttacgcctcc tccagggtca taccggcaaa catctcatcc
    45781 atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa tcccttgttg cggatgttct
    45841 ttatcaacgc cacgcaggaa cagatagata acgccgccaa agtgacgatc atagtcgtaa
    45901 tcagcaatgc gatggcgcag ataacgatgc agcgccaggg tataaagctg atattgcaga
    45961 tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt gggtgtaagc cgaactgtct
    46021 tcacccagcc agttggattt atagtcgagc aggtaataac gcccttcgtg gcggaatacc
    46081 aggtcgataa agccttttaa catgccgcgt acctgcatga actccagcgg cgggcagcca
    46141 gcggatagcg ggtcaaactg gcgaattagt gcatcaagct ggctggcgat gagcggttcg
    46201 ctaatcggca ggtaaaactc catctccacc tgtttatcgc ggtcggaaag ctgactcagg
    46261 ctaacacccg tttcattgag aggtgcctgg aggacagccg tgatccactc ggtcaacacc
    46321 ggttcccact gcgattcaaa gccgccgagt tccagttttt cctgtaccca gttcgggtca
    46381 accggctggg taaaatcgag gtcttcaaac aaactgtgca agaatgtccc cggtgacgca
    46441 ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa cgacgctgac cacgcctgcg
    46501 gcatcaacat ccagccgtgg catcagatcc tgagcgatac cgtgaccacg ctgctgcaag
    46561 ccggagtagc tggtgacacg ccagttatcg ccgggcaatc gttgtagtgt cctcgcattc
    46621 agttctgcag ttaaagcatc attaacctgc cagggctggt tatcaccagt ttgtgccgtt
    46681 tgccaggcaa tatcatcatc gcataacgct tcaatacagg tgcgaagccc tgccgcatct
    46741 tgcggttccc ctttttgcag caaacgcccg agcgcacttt ggtggacgtc ggtgtcacct
    46801 tttttatcgc cacgacggcg caccagcggt gcaacgccga gactgcaatg ccaaaccgaa
    46861 cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca gacgttcgac ctccgcgagg
    46921 tcgacgcttt ctggcgcagc attaagatcc aaaactgcct caaacgagtg gcgatcgtga
    46981 taaaacgcct gatcctggac gcggaaattg gtaataaatg gcaaccagac cagcggatat
    47041 tccagccctt tcgatttatg gatcgtgaca atctgcacca gatgtttatc actttcgagg
    47101 cgcatttgtt ggctggaggc attactgtct ggctcgagga tatgttgcga taaccagcgt
    47161 accagcgcat gttcactttc cagctgcgtt ccggcttctt gaagcagttc gctgatatgc
    47221 aagatatcgg taagacgccg ctcaccgcct gccgttgcca gcaagttttc tgcaatgtta
    47281 cgcgccgaca tcagcgcccg cagcatcggc ataacgccac gtttgcgcca gatttgccga
    47341 taaccatcga actcttcgac taccgcatcc cacgcatgtt cgtcattatt cagcgtttca
    47401 atatccagcg cattcagccc catcattgac gttgccagcg cactgcgcag ggtgttctca
    47461 cgttcgggcg tcatcaccgc ctgcaacagc caaagcattt cctgcgcttc cagagtttca
    47521 aaaacactat cgcggttcga aaggtaaacg gaagggattt ccagcaacgt taaggcatcg
    47581 cgcacctggg cggcctcctg gcggctgcgc accagcacgc tgatgtccga agcacgcacc
    47641 ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc gctgtccggc ttgtagccag
    47701 tcgcggattt gcgcagcaca tacctgcgcc atggtacttt gatagtcgcc aacgccgcag
    47761 ctttcgcctt ccatcagcca cattttcatc gcaggctgcg tttcaccttt aaagacaaaa
    47821 cgtaacgcct gatttttccc ggctgatttc actggaataa acggtatttc gcgaaacatg
    47881 aacgcgtcat cagtctggct gaaaagcttg ttcacactgt tcaccattcc aggtgcggag
    47941 cgccagttgg tgtctaaagt gtagtgggcg tgaacttcac tacgcgcctt catataagtg
    48001 aagatatccg caccccggaa tgcatatatg gcctgcttcg ggtcgccaat cagcaacaat
    48061 gcggtttctg gctgatggtg ccagatacgg cgaaaaattc ggtactgctg ggggtcggta
    48121 tcctgaaatt catcgatcat tgccaccggg aatcgcgtac ggatcgccgc tgccaacacc
    48181 tcgccgcttt cgctacgcag cgcggaatcg agccgactta acatgtcatc aaaacccaat
    48241 tcgccacggc ggcgtttttc acgcgctact gtttcgcgga tctcagccaa tgcgcgggtg
    48301 atcaccagat cacggatcga caatggttct gcaagcagtt gatcgatcgc ctcaaacagt
    48361 ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt ctaagaaacg ctgggagaat
    48421 ttttccagcg actccggcaa ctgataactg tttgtctctt cttctgccca ggcgctgatc
    48481 ttgtcgatcc atttagcctg attgctacgg ttaaacttgc gtcgatcaat accagaagat
    48541 tcgatcagcg catccagttc acccactgcg tcgcgccact gctgttttac cgtatcaata
    48601 cgcgccacaa tttgcgcgtg acgggaagct agcgtttcat catcgggcgg cggtgctttg
    48661 ataaccggcg cttcgccttg cagataacga ttaatatcgc gcagcaacgc ctgcggccct
    48721 ttccaggttt caaagacgac ctgggctatt tcacgcggca gcgggtagca gtggcgacgc
    48781 cagaaatcgg cgcaggcctg gtagcgtagc agagactcat cttcaatcag ctgctgctca
    48841 aacagcatgc cggattcaaa ggcattcagg ttgagcatgc gctggcaaaa gccgtgaata
    48901 gtaaagactg ccgcttcatc catctgccgt tcggctaaca acaaccactg cgcggcttgc
    48961 gctttatcgt cgatctcttc cagcaggtgt tcgtacaatg gattgtcggt ggtttcacgc
    49021 agacaggcga tgcgcaactc gtggatattg ctacggatac gaccgcgcaa ttctgccgtg
    49081 gcagcctcgg taaaggtgac caccagcagt tcttcaacgg tcagcgggcg gggaaaggcg
    49141 gcggaaccgc ctagtccaag taacaggcgc aaataaagcg ccgcaatcgt aaaggttttg
    49201 cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta agggcaagcg caaaggatct
    49261 agtgtctcgg cgacatcact cattcttttc actcatcagg ggcattgttt gctgcaacgc
    49321 gctgacgttc tcccacactt tccagccttc aggatgcaca tattcggctt tcccgttttg
    49381 gctgccggaa atctgcgaca ggatagccat gccttgcggc tcgactaccg cctgatggaa
    49441 gaaatcggcc agtttttgcg gcgtcagcag tttaatctgg gccacgattt tatcacgcga
    49501 atcgaagcgc atattgccgc gatcgaaatc tttacttaac ttcgatgctt cttcgccgag
    49561 cgtttgcggt gcctgcagca tctgggtaat taccgcctgc tggatttgcg caaactcatc
    49621 tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa aacgccttgt aacgctccca
    49681 caagaatgaa ggctgtttat cattgctttg caaaaggaag cccatgcccc actgacgccc
    49741 cacgctcatt ggaaacgcaa acacggcata gcccaattgt tcttcggtac gcaactgatt
    49801 gtagaaccac ggctgtacga tctgccccaa cagagagcta taggctgagc tggtgtattc
    49861 atcgtagcca gtcggtacaa ataccgctgc cagtgcggag tcggtgctgt taccggcttt
    49921 ttcaaagatg acggattgtt ttttatcgac cactacatct ttgtttcgac accactctga
    49981 accatcagcg cccaactgtt tttgcacatc gcgtgccagc gttgttgcct gggcctcggt
    50041 catgttgccg ataaccataa actctggtcg agcccctgat tttaaggcgt cgcgataagc
    50101 cagcacctct ttcaacgtaa tggagggcaa aattttacgc cgttcatctc gcgagaagta
    50161 cggcacttgc gagagcatct gcgcgggcat aatcgcctgc tcaaacgctt tacccttttc
    50221 tgcggaatcc atcatctggt tataccagga cttcgcctgc tcaagctgat cttccgtagc
    50281 ggtatagcta aagtacccct cgagcaatgc ctggaacagc tgcggcagac gctgggtgta
    50341 accattagca ttaaccataa ggccgttgtt agcgttggtg gaaaaactta tgccaccaac
    50401 cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg agataatcat tgagcgcaaa
    50461 catcacctga ttgcgggcgc tgtccatggc tttcggatta cgcaaaatca ggctgacatc
    50521 agctttgggt tcgctggaaa aataacggct tggcgcatac accacgcgca gattcgactc
    50581 atcaacaatc agctctggat ggtcatattt cttctctgac ttaatcagcg agaaatcgtc
    50641 aggaatatag gggttaagct ccggtaagga gagcgcaata ttggcagctt tttgctgcca
    50701 gtcagcgaaa gtttgttcgc tgattttatc gacctgatac ggcgcatcga caaaataagc
    50761 cgttttattg tgcggctctt tcgggctgat ataccagata cgcgcattct gcggcgtcat
    50821 catcgccaga cgttctttta ctgctttagc atcgtaccga tcggcaatat tgactgcatc
    50881 cagcgtatgc tcaaccggaa cgcgaatcat ggtatctgcc agccattcga cgtaatccat
    50941 atcacgggtg atcgacggat aacggaagtc gatatccagc acattcgcca gttcatcgaa
    51001 gtattgttta tcaatgcctt tttcacgtaa cagattgaga tagctaaaaa ttgccgccac
    51061 aacctgatcg cgattagcca ggcctttatc ggttaaagac gcagagatcg ctaatacgcc
    51121 gctgttgccg ttgacgatag gatcggagtt ggcgctaatg ccctcaacta atccctgctt
    51181 ttgcagccag tcggaaagtg tacctgggct gcgattacca atcagatagg taatcaattc
    51241 atccgtttta ctacggaact tcgctgagtt gttatcgatg cgaaactcaa cgcgcagaac
    51301 tttacgcggc attgccggga cgtaatgaat gataatgccc ttttgcgcgt cggtgactac
    51361 cggcacggtg atttccggtt ttttgctctc tttgttcggc acgcgaccaa aggtgtccgc
    51421 cgccattttt gccaactccg gcagcggttt attgctgtaa ataaccgcct tcatcagatt
    51481 ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc tgcaccggat taccaggttt
    51541 atcgcttaaa gtttcgaggt taccaccaga aaactttgaa ccggggtgtg ccgggttaat
    51601 ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc gtacgcgcca tggttaattc
    51661 agcgttcacc gcattacgct cacgttcggc atatttcttg tcgagcaaag gttcagcaat
    51721 agcatcggcc aggcggtcta ccgcaccagg caaggcgtcg ttctcaactt ccagatagaa
    51781 agccgtgcga tacggcgcag tgctggcatt gtgactaccg ccgtgcattt tgagatattc
    51841 ggccagactg tcagcctgcg ggtacttttt cgaccccatc agactcatat gttcaaggta
    51901 atgtgccagc ccctggtacg cctcgggatc ttccagcgac ccaacgggca ccaccagcgc
    51961 cgagagcgat ttaactgcct gcggatcaga aaccagcaag accaccatac cgttatccag
    52021 acgtatagcc tgatactggc ggttatcttt atcactttta cggatggttt cctgaatcgg
    52081 ctgccatccc gtttctgcct gacttaaggg tgcccaaagg gcaactaaca acaataatgc
    52141 tttgaaccag gtgctgcggg gcattctcgg acctcataat caacttaatt ttctgtccag
    52201 attcaacacg ttaacgcaaa tcatctgcca gaatttaatc ttgtgctgca cgagtcagcc
    52261 tatgtttata taaccatcag tccgtgactg gtgcgcatca taaagtaagc ggatagattg
    52321 cgcaattttt atacagcact catgactgat taaagcgaaa cagcggtaac aggaaacgtt
    52381 gcgactgttc aacgatggcc tccattgtct ctggtgttaa ttgccgccag agcctttgat
    52441 accagatatc atcaccttcg ccacgcacca tcatgttgcc ttcgtaagcc tgaaggaatt
    52501 tcgtacgggc tttttgcaac gtggaatcgt catccagcat ggcatcgttt tgcgcgtcat
    52561 aacaggtttt tagccacgcg ccgccacttt caggtaacac cagcaatggc gcggacatcc
    52621 cttcacgata cccctcaatc agttgtgaga ggtaatgcaa agcctgttcg gctgcaagcg
    52681 gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg actttcacca ttaccaccgc
    52741 tggcacagta gacaaggtgt tccagccaaa gttgcattcc ctgcgccaca cttaataaag
    52801 agggacgcca gcgcaacagg ccatccggct gcacctgcgg caaccagcca gttatctgca
    52861 caccgttgca ggcgagatca atttccatac tctgccccgg ctggcgacag gcaatgactc
    52921 tgtcggcaag ctgctgcatc tcctggcact gagtttccca gaaaatttca ccaaaagcgc
    52981 catacggtaa atcccctgcc gcccggaagc gacggaacaa gcgttcggca tcatcctgct
    53041 caaccagtgc attcaataac tgctgattga tttgataacg gctaagccct tccagaataa
    53101 atggctcggt gtcggggatt tcgctgtctt cagtacggaa gttcacctgc aaacgcatct
    53161 ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag cgtttccagc ggcacggttt
    53221 ccggtaaggt aaacggcagc ggctgaacaa attcagaatg tgctttacca gcctggctgg
    53281 ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc tggctgatag ttttgtggat
    53341 caaacggcat tcgggtatgg aggcaagtaa gatgcgcttt tacccttgcc tcgctttcat
    53401 cacagttgag cgcttcatcg cccggtagat aatgactttg cccgatgtag tcgatcagtt
    53461 cctgcaccag taccgacggg aaacgttcac tgttatcctg aatggaacgc ccgatatagc
    53521 tgatatagag tttttgctgc gcggaaatta acgcttccag gaacagatag cggtcgtcat
    53581 cgcgacggct acggtcgcca cgcttcggtt tctggctcat caggtcaaag cccaatggcg
    53641 caagctgacg tggataaacg ccgtcgttca ttcccagcag gcaaaccact ttgaacggaa
    53701 ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc ggcgagaaaa cgctggctga
    53761 tacgttcttg atccagacgc tgtgccagtt catcacgcaa tagtgacagc ggcaccgcgt
    53821 cgccatactg cgcacctaaa ccttcggcga taatcgcctg ccattgttgt tcgatcagcg
    53881 tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc gctgagcata tcgcgacaaa
    53941 ccggcaacca ctcttctagc ggacgctcct gtgccagccc gcgacgccag atgtttagct
    54001 gcattagcag tgaagccaga tgccccacca gttctgcaat taagccgctc gattcatcat
    54061 aaggtagaac cgattgccac tcgccctgcg cgctctccat cgcgtagccc agcaacatac
    54121 gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg gagttccagc tcgcgaacgt
    54181 tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac ccactggcgt aaataacgca
    54241 gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg cacatccagt aatgccagca
    54301 cgtcttccga cacaaagcgg ctgtctggca acgataacag gctgataaac gcttccagta
    54361 ccggatgcga ctgccgcgcc cgacggtcgg aaatggcgta aggcaggtaa cgatccgcag
    54421 gtgcgctacc aaacacagcc cgaataaacg gactgtagct gtcgatatca gccaccatca
    54481 cgatgatgtc gcgcggagta agtgtcgggt cttcttccag catcgccagc aggcgatcgt
    54541 gcaaaacttc aacttcacgc tgcgggctat ggcaaacgtg gaaggtgata ctgctatcca
    54601 gtggatcaag cgggcgtttg ttatcgctac gggaaaactc ttcgatgttc acaccagcaa
    54661 cggcacggtt ttccagttcc agaatgtcag actgaatgtt atgcagcagg ttatctggcg
    54721 tgacatcgac aaaggcgtcc agctcctggc tgctctccag gtcagaaagg agataaatgt
    54781 agtcgcgccc aagcttaccc catgaagcca gcagcgggtt gccgacatcc tgttcaccat
    54841 cgctgttaaa gagctgcccg gcattttcgc tatcacgaaa tagcggcaat tcgcgatctt
    54901 caaaactgtg tcgacgctga cgggtcagca gtttcgccag ataagcagga tctttaatat
    54961 cgccccagta ataacggcag gggttggtaa acaggagatg gatttcaata tgtttaccca
    55021 gcgcctgtag cgcctggaga taaacaggcg gtaacgcgga aataccgcat ataaagacgc
    55081 gcgaaggtaa ccccggcggg caggtcgtcg cggactccag cgtttcgata aagcgctgat
    55141 agagattggc gcggtgccag cgcggttgcc cgagttgatg ggtatattcc accagcgcct
    55201 tccacaacgg ggcttgccag gcctgtgctt ctcccagccc ttcaaccaaa tgtcctgttt
    55261 cccactgtgc cagccagtcc ggacgataga ccagatactg gtcaaacagg tccgccgctt
    55321 ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc agtcagataa tgccgcaaca
    55381 gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt catcagtttc cagctcatgc
    55441 tctgtttgtt aaaggcgctc tctttgggga tttccggtaa cacccggacg aacatatccc
    55501 agataaagct cgctggcagc ggaaaatcaa tgtttgccgc aatactaaac ttttgcgaca
    55561 gggtcatttg cagccactgc gccataccgg tactttgcac cagaatcatc tctggttcga
    55621 aaggatcgtc cagccgttcg cgttcgacaa taaactccat caacgcttcc agcacgtcca
    55681 gacgattgga atggtagacc cttaacatag cggctcctga ctactgacga ttcgggcaat
    55741 gcaggcgggt catctcgcct tctctgcccc cgggtgaaac tagcgtaacg ctgatgctga
    55801 cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca gttggcaggt ggcgaaatcg
    55861 cgcgcagttg cgtttgctgc caaccatgcc gccagagctg ttggtactgg tttctgctgg
    55921 cgagactgtt cattaatgtt cgctggatac ccgataacgc agtgacaatc atcaccatca
    55981 gcaccatcgc aaccattacc tccggcaggc taaagccttg ttgattcttc agggaagctg
    56041 acataacgcc ctctctttca gcggacaaaa atcgctccag ccgcgtggcg aaaaaacaat
    56101 gtttccatca atgacttcgc ctgttcgcca caacgaaacg ccttcataac cggcaaccaa
    56161 taaggcttca ttatctgcca gtaaacgcaa acaaacccgg gcatcggttc cagcgtactg
    56221 cgagcactga actgcgggct gcgtctgcca ggagtgcatt ttcccccacg ccagcgccga
    56281 ctgaacgatg gcctggcggc gcaatgactg actttctatg ctcacgcgag aggcaaaact
    56341 gcgatcctgc tgactcattc cttgcaatag caagctaccc aaaaccagca gcatcaggac
    56401 cagagccagt gacgaaacac ctttttcgcg gttcacaggt tgaatcctgt cacgctatag
    56461 ctggcatcca ccacggtttg cggttcagac ttactggcag cacgcatatt aaccgtcaac
    56521 accggcgaga agccgctgac atcctgacgt acgacctgaa aagtgtcgat aatgatggca
    56581 tccggattag tgactttatc ccagccctta ccttcacagg atgtcgcacc gcgtagcgtt
    56641 tccagcacat gctccttcag acgaaatcca atctggtcag actcttttac cggttcgcga
    56701 tcccagatac cgttactgtt cgcatcccac tgcacaatga tacagtcacc ctgtccgaca
    56761 atttccagcc cttcgccggt acagctgcca tgacaataac ccgccctctg gagatgcttc
    56821 gcgacggtaa atacccgcag ccagatttca tcttccagcg ccagcttacg ggtgctcgtt
    56881 aaactttcac gctgtaacgc aggcagaaag cgtgcagccc ccagcaacaa tacgctactg
    56941 atcgccatag caatcaacac ttccagcaga gaaaaacctt gctcttttac aggcatcctt
    57001 ctgtttctcc ttgctgacaa agccggagtc ttccccacgg cgaaaccacc agccaccact
    57061 cgcccgttga gtttttgaag cgaatatgcc cggcccatgc ggtattgcgc aggccaaaga
    57121 aagcaagcga aggtgtcagg tcgctcattt agacttcggg ccagcgtggc acaaagacca
    57181 atggtgaact gccatgacag gtattggccc cagcagcgga actcacaagg caccataacg
    57241 tcccctccct gataacgctg atactgtggt cgcggttatg ccagttggca tcttcacgta
    57301 aatagagcaa atagtcccgc gcctggctgg cggtttgcca tagccgttgc gactgctgcc
    57361 agtattgcca gccatagagt ccacttgcgc ttagcatgac caaaatcagc atcgcgacca
    57421 gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc ggcagtatgg agcgaggaga
    57481 aaaaaagacg agggccagtt tctatttctt cggcgcatct tccggactat ttacgccgtt
    57541 gcaggacgtt gcaaaatttc gggaaggcgt ctcgaagaat ttaacggagg gtaaaaaaaa
    57601 ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc caccggcgct
    57661 ttaatgcccg gatgtggatc gtagccttca atctcaaagt cttcgaaacg gtagtcgaag
    57721 atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc gcggcttaat
    57781 tgcagatgag tttgatccat atgattgctg tacaggtgcg tatcgccacc ggtccagaca
    57841 aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa cgcgtagctg
    57901 gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag ctggcaagag
    57961 agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag cgccatttta
    58021 tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gggaatccgg gtcgtttttc
    58081 agctggttca gtaccgtagt gatctggtca atatgacgac catctggcgt tggccaggcg
    58141 cgccactgtt taccatacac tggtccgagg tcgccgtttt catcggccca ttcgtcccag
    58201 atggtgacat tgttttcgtg tagataagca atgttagtgt cgccctgcag aaaccacagc
    58261 agttcgtgga tgatggaacg caggtggcaa cgtttagttg tcaccagcgg gaatccatcc
    58321 tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg
    58381 tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg tttcatggtt
    58441 cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat gatcacaccc
    58501 gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac ccaggcaccg
    58561 gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc gccgtagcca
    58621 atcaggaaca agccagagac agctcccatt gggcgcggtt tacgaatata caggttgagg
    58681 ataataaaca gcaccacacc ttccagcagc agctcgtaaa gctgtgatgg gtggcgcggc
    58741 agcacaccgt aagtgtcgaa aatggattgc cactgcgggt tggtttgcag cagcaaaata
    58801 tcttctgtac gggagcctgg gaacagcatg gcaaacggga agttcgggtc aacgcggccc
    58861 cacaattcac cgttaataaa gttgcccaga cgcccggcac caagaccaaa cggaatgagt
    58921 ggtgcgataa aatcagaaac ctggaagaag gaacgtttag tacggcgggc aaagataatc
    58981 atcaccacga taacgccaat caggccgccg tggaaagaca tgccgccgtc ccagacacgg
    59041 aacagataca gcggatcggc cataaactgc gggaaattgt agaacagaac ataaccaata
    59101 cgtcccccga ggaagacgcc gaggaagccc gcatagagta agttttcaac ttcatttttg
    59161 gtccagccgc tgcccggacg attcgcccgt cgtgttgcca gccacattgc aaaaatgaaa
    59221 cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga gaaaatgacc
    59281 ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa gttcttgtta
    59341 tttcgctgaa agagaacagc gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt
    59401 gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaag atatgtatat
    59461 gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct
    59521 gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca gactacgctg cgcaagattt
    59581 tctgcttcgg cataatcaat gcgccgcagc aggtatttta cccgcgctac agaacgtccg
    59641 ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat gggatcgccc
    59701 gccatttcac cgcacaaacg gagatcgatt ccatgtattt ccgcttcccg ggcgatcatc
    59761 gccagagctc gtaacattgc aggatgaaga ctgtcataaa tgttcgccac ccgggtattg
    59821 ttgcgatcaa cggccagaat gtattgagtc agatcgttgg tgccaacaga gatgaaatcg
    59881 acccgctttg ccagatgcgg cagcataaat accattgacg gcacttccag catgatgccg
    59941 atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc ggcacgttca
    60001 atcaggcggc gtgcttcatc aacttcatcg aggcttgtga ccatcggcaa cagaatattc
    60061 aggttgcccg tagcggcatt agcacgcagc atcgcccgca cctggatcaa gaagatctcc
    60121 ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc ttcgctgatc
    60181 ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt gacgggttta
    60241 tcattgaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc cgacgggaaa
    60301 ccactttgca gcatgaatgg gatttcagtg cgataaagac cgatgccatc aatacggctg
    60361 cccagttttt cttcatgttc cgggcttaaa ccagcattga gcatgacttt tatacgctca
    60421 ccgcttttta actgcgcggg taaattgacg tcatcttccg ccagacggct aagctcaatc
    60481 tcttcactaa ttagccgctg atattcttgc agcagtaccg gctccggatc gaccagcaat
    60541 tcaccgcgat agccatcaac gatcagcgtc ctacgatgca gcaccgaagg ctgaatatcc
    60601 gcgcccatca cggtagggat ccccagcgca cgtaccatga tcgcagcatg ggagttggct
    60661 gcgccatctc gcacgacaac accgactaag cgatcctggg gcagctcagc aagagttgtc
    60721 gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg cccttgatta
    60781 gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcgcc agcccgctct
    60841 ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat gaccgttttt
    60901 accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg acgcagccgg
    60961 gtgtccgaaa gtaggtgcga gtaaagatcg aaaatagccg ccgtttcttt ttgtgctccg
    61021 gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg cttcttccag cgccccggtc
    61081 agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg ttccattaaa
    61141 ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc cggtaatgcg
    61201 cggattcgcg tctggcgata ttgcccaaac aaggcagtca actgcgactg agaaagaata
    61261 gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc atactggcgc
    61321 aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat aattggtacg
    61381 cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa gctggggtgc
    61441 ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc gacgatccct
    61501 tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagcccccg ggtcgccatc
    61561 aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc ggtatccatc
    61621 gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg tgcgctggct
    61681 accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta acctctttta
    61741 cgtcgataag cagatgtgtt ttgtggtttt ggcgtatttt cctgcagtga catcaccaca
    61801 ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac cacctgtctg
    61861 accggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact gctggtttgc
    61921 atattgattt ctgcatcgcc gctcaccagc tgcaagagaa accatttttg tttttggccg
    61981 atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag
    62041 ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc ttcaaacaat
    62101 tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg cggaaattgc
    62161 caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt acaaatcacg
    62221 atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca aaataaagct
    62281 ttatatacga atgattgttt catactccaa gaagacggta aaccactctc tgcagggcat
    62341 tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa cagaggaagg
    62401 ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa ctacggaatt
    62461 actacgggaa aacccggagc attctgaata agcccgatat agacatttta aaatattcat
    62521 tattagtcaa tgcattacat gtaatttgag taatgaaaaa gagtaattcg tgacccaggt
    62581 cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg
    62641 tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt
    62701 aaatggaaac agtagtggag gtttttcaca gttatcccag ctttctgtgg ataacatggt
    62761 gtaagatcct gtttattttc agtgaccaga tttggaaaac ctattgcagt gttgcgcaac
    62821 tcgattaccg gcagcctaaa aagcaatata aatcagagaa ttgaacaaca catgtggaaa
    62881 aagttacact gcgaatattc ggcacataat tgctgtttgt tttttaacca aggtatcatg
    62941 acatgtccca acctcgccca ctcctctctc ctcccgaaac tgaagaacag ttgttagcgc
    63001 aagcacagca actttctggt tatacattgg gagaactggc ggcacttgcc gggctggtta
    63061 cgccagagaa tttaaaacgc gataagggct ggattggcgt gttactggag atctggctag
    63121 gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg gaacttaaaa
    63181 ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt
    63241 taacgggtaa tagcggggtg acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg
    63301 tactgtggat accggttgaa ggtgagcgca gcatcccgct ggcgcagcgt cgcgtaggat
    63361 caccgttgct gtggagcccg aatgaagagg aagaccgaca gctacgcgaa gactgggaag
    63421 aattaatgga tatgattgtt ctcggtcagg ttgagcggat caccgctcgt cacggggagt
    63481 atttacagat acgaccgaaa gcagcgaatg cgaaagcgct taccgaagcc attggtgccc
    63541 ggggcgaacg gattctgacg ctgccacgcg gcttttattt gaagaagaat ttcaccagtg
    63601 cactactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc gctttccggg
    63661 catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg catggataac
    63721 cgatcctaac gcctggcttg cgctcggtac gctgacgctg ctggagatcg ttcttgggat
    63781 cgacaatatt attttccttt ctctggtggt ggcaaagctc cccacagcac aacgtgctca
    63841 tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt ctggcgctgc tggcatcaat
    63901 cgcctgggtt acgcgcctga caaatccgct ttttacaata ttcagtcagg aaatttccgc
    63961 ccgtgatttg attctgcttc tgggtggctt gttccttatc tggaaagcca gcaaggaaat
    64021 ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg
    64081 cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc
    64141 tgtgggtctg tcagatcacc tgtttattat gatggccgcc gtggtgattg ccgtaggcgt
    64201 gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat
    64261 gctggcgctc tctttcctga ttctggtcgg ctttaccctg attctggaaa gtttcgacat
    64321 ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct
    64381 caacctgatt cgcaacaaaa agaacccgct ctgatactcc gtacgctctc ctgcgggagg
    64441 gcgtactcac tcctgttacc ctcacagttt aagattttcc tgctttcaag atatataacg
    64501 tcggtttata aacagactat tatcataggt gggccaaatg aggataaccg gatgaaaaaa
    64561 tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat
    64621 tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat
    64681 gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca aattaaccgt
    64741 gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatccgg ctgtaatgcc
    64801 gatcagttaa g
//
LOCUS       DAQSWQ010000010        60957 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.010, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000010 DAQSWQ010000000
VERSION     DAQSWQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.010"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..468
                     /locus_tag="R8G18_000655"
     CDS             <1..468
                     /locus_tag="R8G18_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9794442.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="R8G18_000656"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="R8G18_000656"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEE9794443.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="R8G18_000657"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="R8G18_000657"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEE9794444.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="R8G18_000658"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="R8G18_000658"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HEE9794445.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="R8G18_000659"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="R8G18_000659"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEE9794446.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="R8G18_000660"
     CDS             complement(4084..5313)
                     /locus_tag="R8G18_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEE9794447.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="R8G18_000661"
     CDS             complement(5377..5817)
                     /locus_tag="R8G18_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEE9794448.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="R8G18_000662"
     CDS             complement(5922..6692)
                     /locus_tag="R8G18_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9794449.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="R8G18_000663"
     CDS             complement(6689..7615)
                     /locus_tag="R8G18_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEE9794450.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="R8G18_000664"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="R8G18_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEE9794451.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="R8G18_000665"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="R8G18_000665"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEE9794452.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="R8G18_000666"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="R8G18_000666"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HEE9794453.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="R8G18_000667"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="R8G18_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HEE9794454.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="R8G18_000668"
     CDS             13322..13669
                     /locus_tag="R8G18_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HEE9794455.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="R8G18_000669"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="R8G18_000669"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEE9794456.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="R8G18_000670"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="R8G18_000670"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEE9794457.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="R8G18_000671"
     CDS             complement(15489..15851)
                     /locus_tag="R8G18_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HEE9794458.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="R8G18_000672"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="R8G18_000672"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEE9794459.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="R8G18_000673"
     CDS             18978..20735
                     /locus_tag="R8G18_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEE9794460.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="R8G18_000674"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="R8G18_000674"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEE9794461.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="R8G18_000675"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="R8G18_000675"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEE9794462.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="R8G18_000676"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="R8G18_000676"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEE9794463.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="R8G18_000677"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="R8G18_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HEE9794464.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="R8G18_000678"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="R8G18_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HEE9794465.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="R8G18_000679"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="R8G18_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HEE9794466.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="R8G18_000680"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="R8G18_000680"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEE9794467.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="R8G18_000681"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="R8G18_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEE9794468.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="R8G18_000682"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="R8G18_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HEE9794469.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34428
                     /gene="gspE"
                     /locus_tag="R8G18_000683"
     CDS             33043..34428
                     /gene="gspE"
                     /locus_tag="R8G18_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HEE9794470.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34418..35620
                     /gene="hofC"
                     /locus_tag="R8G18_000684"
     CDS             34418..35620
                     /gene="hofC"
                     /locus_tag="R8G18_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HEE9794471.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="R8G18_000685"
     CDS             complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="R8G18_000685"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEE9794472.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36854..36898)
                     /locus_tag="R8G18_000686"
     CDS             complement(36854..36898)
                     /locus_tag="R8G18_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794473.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36923..37543
                     /gene="coaE"
                     /locus_tag="R8G18_000687"
     CDS             36923..37543
                     /gene="coaE"
                     /locus_tag="R8G18_000687"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEE9794474.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37543..38286
                     /gene="zapD"
                     /locus_tag="R8G18_000688"
     CDS             37543..38286
                     /gene="zapD"
                     /locus_tag="R8G18_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEE9794475.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38296..38493
                     /gene="yacG"
                     /locus_tag="R8G18_000689"
     CDS             38296..38493
                     /gene="yacG"
                     /locus_tag="R8G18_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEE9794476.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="R8G18_000690"
     CDS             complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="R8G18_000690"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEE9794477.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39042..41747)
                     /gene="secA"
                     /locus_tag="R8G18_000691"
     CDS             complement(39042..41747)
                     /gene="secA"
                     /locus_tag="R8G18_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEE9794478.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41809..42321)
                     /gene="secM"
                     /locus_tag="R8G18_000692"
     CDS             complement(41809..42321)
                     /gene="secM"
                     /locus_tag="R8G18_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HEE9794479.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="R8G18_000693"
     CDS             complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="R8G18_000693"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEE9794480.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="R8G18_000694"
     CDS             complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="R8G18_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEE9794481.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="R8G18_000695"
     CDS             complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="R8G18_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEE9794482.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="R8G18_000696"
     CDS             complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="R8G18_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HEE9794483.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="R8G18_000697"
     CDS             complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="R8G18_000697"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEE9794484.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47786..49261)
                     /gene="murC"
                     /locus_tag="R8G18_000698"
     CDS             complement(47786..49261)
                     /gene="murC"
                     /locus_tag="R8G18_000698"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEE9794485.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49315..50382)
                     /gene="murG"
                     /locus_tag="R8G18_000699"
     CDS             complement(49315..50382)
                     /gene="murG"
                     /locus_tag="R8G18_000699"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEE9794486.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="R8G18_000700"
     CDS             complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="R8G18_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEE9794487.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51623..52939)
                     /gene="murD"
                     /locus_tag="R8G18_000701"
     CDS             complement(51623..52939)
                     /gene="murD"
                     /locus_tag="R8G18_000701"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEE9794488.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="R8G18_000702"
     CDS             complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="R8G18_000702"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEE9794489.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54018..55376)
                     /gene="murF"
                     /locus_tag="R8G18_000703"
     CDS             complement(54018..55376)
                     /gene="murF"
                     /locus_tag="R8G18_000703"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEE9794490.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55373..56866)
                     /gene="murE"
                     /locus_tag="R8G18_000704"
     CDS             complement(55373..56866)
                     /gene="murE"
                     /locus_tag="R8G18_000704"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEE9794491.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="R8G18_000705"
     CDS             complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="R8G18_000705"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HEE9794492.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="R8G18_000706"
     CDS             complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="R8G18_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEE9794493.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="R8G18_000707"
     CDS             complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="R8G18_000707"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEE9794494.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="R8G18_000708"
     CDS             complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="R8G18_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEE9794495.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14595 a        16851 c        15002 g        14509 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcgacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaagc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat taaagccgcg
    33961 caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta
    34021 cgtttacagc aaatgggagt cgcccgctgg atgctctcat cagcgcttac gctggtaata
    34081 gcccagcgtc tggtacgtaa actttgccca cattgtcgcc agcagcaagg ggagcccatc
    34141 catattccag tcaatgtatg gccgtcgccg ctgccccact ggcaggcacc cggttgtgta
    34201 cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg
    34261 gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga
    34321 caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc
    34381 acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg
    34441 ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg
    34501 tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat
    34561 cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc
    34621 gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca
    34681 tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat
    34741 tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct atcaggcgat
    34801 gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca
    34861 gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat
    34921 cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt
    34981 tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac
    35041 gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc
    35101 gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct
    35161 gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc agatctttac
    35221 gattctggcg ctgacacaaa gtgcaggcat tacttttttg cagggcgtag agagcgtcag
    35281 agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag
    35341 taacggtcat cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt
    35401 gcaattagtg agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc
    35461 ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc
    35521 gacgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc
    35581 aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca
    35641 ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata
    35701 aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa
    35761 cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta
    35821 acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca
    35881 gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga
    35941 ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg
    36001 ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg
    36061 cccagaccgt gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg
    36121 cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca
    36181 gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg
    36241 gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa
    36301 ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta
    36361 gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca
    36421 gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca
    36481 gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata
    36541 tttgcggcga taatcggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga
    36601 cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg
    36661 tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg
    36721 gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc
    36781 aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg
    36841 agcgaacgtt tcatcaattt cgctttcgtg cactcatttt taacgttttg ataatcatga
    36901 tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag
    36961 tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga
    37021 tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca
    37081 ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg
    37141 gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca
    37201 gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt gggttgtgcc
    37261 attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag
    37321 cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga
    37381 acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat
    37441 tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta
    37501 tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt
    37561 tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag
    37621 caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat
    37681 gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa
    37741 cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc
    37801 cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg
    37861 cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc
    37921 atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc
    37981 caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag
    38041 gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagt
    38101 ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg
    38161 ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt
    38221 atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt
    38281 tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg
    38341 gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc
    38401 gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa
    38461 agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtaggcct
    38521 gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac
    38581 gccttatccg acctacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa
    38641 atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca
    38701 cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa
    38761 ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg
    38821 aagttcacgc actaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa
    38881 ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt
    38941 ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa
    39001 aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg
    39061 cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc
    39121 tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc
    39181 tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc
    39241 tcttcaggca tacgtacctg gactttgctc agcgtgctga taacttcata ttttaacgac
    39301 tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc
    39361 tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgccgc taggtgctct
    39421 ttccacagag agtcgagagt ttgcagcatg acgccttttt cgaagtgacg catcatctca
    39481 gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag aattcgctca
    39541 cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg
    39601 aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagt
    39661 gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg
    39721 atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga
    39781 cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacgg
    39841 ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct
    39901 tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata
    39961 cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga
    40021 ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg
    40081 atatgcaggc cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt
    40141 ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca
    40201 ccgagcacaa tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga
    40261 taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg
    40321 tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata
    40381 gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc
    40441 tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga
    40501 cggttggtcg gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg
    40561 gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag
    40621 gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca
    40681 gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca
    40741 acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga
    40801 gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac
    40861 tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt
    40921 tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag
    40981 gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta
    41041 tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg
    41101 atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct
    41161 tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg
    41221 taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg
    41281 ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc
    41341 gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa
    41401 gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt
    41461 tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc
    41521 ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct
    41581 ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt
    41641 tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg
    41701 cgatcgttac gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa
    41761 acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg
    41821 ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc
    41881 ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg
    41941 ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga
    42001 ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt
    42061 gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt
    42121 cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt
    42181 tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact
    42241 cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca
    42301 gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc
    42361 acaaatctta tcattaaagc ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa
    42421 agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa
    42481 atgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag
    42541 tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg
    42601 tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt
    42661 ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca
    42721 cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa
    42781 cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg
    42841 ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta
    42901 cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg
    42961 ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg
    43021 tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga
    43081 acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac
    43141 ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta
    43201 tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca
    43261 tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg
    43321 aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca
    43381 gggcgtaacg tcagagtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg
    43441 atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc
    43501 gaagtgtact atacattcgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc
    43561 agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc
    43621 tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt
    43681 cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt
    43741 ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac
    43801 gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc
    43861 ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa
    43921 gcccgccgtg atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag
    43981 cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc
    44041 agaacccatc attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac
    44101 gttcatcaaa cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac
    44161 atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag
    44221 tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg
    44281 ctccgcgaat gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac
    44341 ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt
    44401 accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa
    44461 tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag
    44521 tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc
    44581 ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atacgctcgc gcaccatgtg
    44641 ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt
    44701 aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa
    44761 tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat
    44821 ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga
    44881 ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc
    44941 cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg
    45001 agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc
    45061 caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt
    45121 gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg
    45181 cagactccgt ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac
    45241 gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt
    45301 gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt
    45361 gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac
    45421 gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc
    45481 ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt
    45541 cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag
    45601 tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg
    45661 cagcacacga tgttcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac
    45721 atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct
    45781 gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc
    45841 tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc
    45901 tttatccata ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg
    45961 cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag
    46021 ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta
    46081 gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca
    46141 acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct
    46201 acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta
    46261 ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg
    46321 tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct
    46381 tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag
    46441 gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg cacatattca
    46501 accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac
    46561 ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc
    46621 ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca
    46681 ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg
    46741 acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga
    46801 cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt gttcagagca
    46861 gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat
    46921 acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt
    46981 ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc
    47041 tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga
    47101 cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata
    47161 atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat
    47221 cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg
    47281 aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg acattcccac
    47341 actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc
    47401 taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc
    47461 gaccggtaaa ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc
    47521 agatgccatc actccgctgc cggtataagg caagcccatc agctcaagca tgccctgcag
    47581 cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga
    47641 cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc
    47701 acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt
    47761 cccgcccaac aggaccgcaa ttttatcagt catgttgttc ttcctccgga gtttgcggct
    47821 tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga
    47881 gaatcaggtc gttaccgatt aataccggtg ccagcatctc ggctacctgc gcagggtccg
    47941 gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt
    48001 ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa
    48061 cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg
    48121 gctgaaacag cattactagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg
    48181 cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac
    48241 cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtcccctgga
    48301 agctctccag cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa
    48361 ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcgcatcg
    48421 gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt
    48481 ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg
    48541 gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca
    48601 ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat
    48661 ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg
    48721 atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa
    48781 cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt
    48841 agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc
    48901 catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg
    48961 cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca
    49021 cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca
    49081 ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat
    49141 tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt
    49201 gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca
    49261 ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc
    49321 ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc
    49381 gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc
    49441 gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag
    49501 cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg
    49561 tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc
    49621 cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg
    49681 ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca
    49741 gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg
    49801 tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg
    49861 ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc
    49921 attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt
    49981 attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca
    50041 cgcggccaga ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt
    50101 gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc
    50161 tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat
    50221 gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg
    50281 ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt
    50341 tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac
    50401 gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc
    50461 gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc
    50521 ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca
    50581 atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc
    50641 gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac
    50701 cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc
    50761 agtttttgta ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc
    50821 agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg
    50881 gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc
    50941 ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc
    51001 gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc
    51061 aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg
    51121 cctttacgca ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca
    51181 ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctaccc
    51241 actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt
    51301 tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga
    51361 tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc
    51421 attgacgccg aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt
    51481 aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag
    51541 ccttttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc
    51601 aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc
    51661 ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca
    51721 tatcgcccag ctgaacacgc ggagccagca agcgcatcgc ttgttccatg gtttcggttt
    51781 gttctgccac ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca
    51841 aacgcacgtt gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat
    51901 cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg
    51961 tactgccgac gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca
    52021 ccacttcaaa gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc
    52081 gcggcaaacc agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct
    52141 gcccggaaag tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccagg
    52201 tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc
    52261 gttcatcagc accgcgaatc ggcattgtta aggcatcatc agcattgacg acacaaactt
    52321 tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgcaaacca aacggatagc
    52381 ggtccatatg atcttcagtc acgttcagaa tggtcgccgc caccgcctgt aagctggaag
    52441 tggtttccag ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca
    52501 gcatcaacgc tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg
    52561 ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa
    52621 tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat
    52681 cagcggcagc gcttaaggat ggatgcgcct gtgcaatacc gggactggcg acaatcagat
    52741 ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta
    52801 atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag
    52861 cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg acgacatttt
    52921 taccctgata atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag
    52981 catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc
    53041 atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc
    53101 gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag
    53161 gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc
    53221 tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc
    53281 cccgactatc gccgtgcaga caatgaccag ttcccctgcg tgtcgcagat acggtatatg
    53341 caagtagctg gcaaagttca tattgccggt cgcccacgcc accagcgcaa aaccaccggc
    53401 gacaaatacg gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt
    53461 gccagtaccc acaatgacga agtaagccag cagaatgtag aacagcccca gctgcggcat
    53521 cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta
    53581 cagggcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat
    53641 caaccctttg gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc
    53701 gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag
    53761 cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg
    53821 cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg
    53881 caaatgagct atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct
    53941 gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa
    54001 atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc
    54061 atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt
    54121 gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg
    54181 gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt
    54241 gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg
    54301 cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt
    54361 gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg
    54421 gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat agcattaagc
    54481 gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc
    54541 cccggcaacg gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta
    54601 ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag
    54661 aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg
    54721 gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc
    54781 gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc
    54841 gcagcttccg ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca
    54901 agttcaataa ctgcgtaatc gtattccggc gttaagcgca acaacgtcat cggtacaccg
    54961 atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc
    55021 gccgtcatct ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc
    55081 ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg
    55141 attaactgcg gcaggtcgat atccagcgga cggctgacca gtagtgcgcc cgcgccgcca
    55201 gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac
    55261 aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca
    55321 ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa
    55381 tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa
    55441 caatctggta atcttcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct
    55501 gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat
    55561 ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat
    55621 cgtccgtcac caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct
    55681 tacctttatc gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca
    55741 ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca
    55801 ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg
    55861 cggcggtttt cagcagatca gccagtggat agccgagtgc cagcagtgtc gccagcgcga
    55921 gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc
    55981 aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta
    56041 accagcgtcc gtgacagttc ggattaatat gatcttccat tgataccgca accgcgtccg
    56101 gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc
    56161 aatgatgttc agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat
    56221 aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca
    56281 cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct
    56341 ccaccagccc cgccagctca tgctgaacat cgactgcgga accggtagta ttttctgtcg
    56401 ggatcacttt cccaagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa
    56461 gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta
    56521 cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata
    56581 aacgctcatt gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac
    56641 cgtcggtcgc ctcatctttc gctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    56701 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    56761 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    56821 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    56881 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    56941 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    57001 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    57061 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    57121 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    57181 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggatccc
    57241 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    57301 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    57361 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    57421 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    57481 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    57541 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    57601 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    57661 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    57721 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    57781 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    57841 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    57901 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    57961 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    58021 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    58081 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    58141 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    58201 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    58261 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    58321 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    58381 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    58441 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    58501 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    58561 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    58621 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    58681 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    58741 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    58801 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    58861 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    58921 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    58981 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    59041 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    59101 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    59161 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    59221 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    59281 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    59341 gatgtttaaa cttatctttc accggcgttg caacagccac gacttccgcc agttctttgg
    59401 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    59461 aacgctcttc accataggtt ttcaataccc aagcgatatc ggcttcttct gcggtttgta
    59521 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    59581 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    59641 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    59701 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    59761 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    59821 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    59881 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    59941 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    60001 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    60061 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    60121 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    60181 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    60241 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    60301 tgaccgacag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    60361 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    60421 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    60481 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    60541 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    60601 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    60661 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    60721 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    60781 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    60841 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    60901 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacg
//
LOCUS       DAQSWQ010000011        59895 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.011, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000011 DAQSWQ010000000
VERSION     DAQSWQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.011"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="R8G18_000709"
     CDS             complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="R8G18_000709"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEE9794496.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
     gene            1594..2034
                     /locus_tag="R8G18_000710"
     CDS             1594..2034
                     /locus_tag="R8G18_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HEE9794497.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="R8G18_000711"
     CDS             complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="R8G18_000711"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEE9794498.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="R8G18_000712"
     CDS             complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="R8G18_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HEE9794499.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="R8G18_000713"
     CDS             complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="R8G18_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEE9794500.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="R8G18_000714"
     CDS             complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="R8G18_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEE9794501.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="R8G18_000715"
                     /pseudo
     CDS             complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="R8G18_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            complement(7008..7721)
                     /gene="livF"
                     /locus_tag="R8G18_000716"
     CDS             complement(7008..7721)
                     /gene="livF"
                     /locus_tag="R8G18_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HEE9794502.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(7723..8490)
                     /gene="livG"
                     /locus_tag="R8G18_000717"
     CDS             complement(7723..8490)
                     /gene="livG"
                     /locus_tag="R8G18_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HEE9794503.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(8487..9764)
                     /gene="livM"
                     /locus_tag="R8G18_000718"
     CDS             complement(8487..9764)
                     /gene="livM"
                     /locus_tag="R8G18_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HEE9794504.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(9761..10687)
                     /gene="livH"
                     /locus_tag="R8G18_000719"
     CDS             complement(9761..10687)
                     /gene="livH"
                     /locus_tag="R8G18_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HEE9794505.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(10735..11844)
                     /gene="livK"
                     /locus_tag="R8G18_000720"
     CDS             complement(10735..11844)
                     /gene="livK"
                     /locus_tag="R8G18_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HEE9794506.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            12268..12651
                     /gene="panM"
                     /locus_tag="R8G18_000721"
     CDS             12268..12651
                     /gene="panM"
                     /locus_tag="R8G18_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HEE9794507.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="R8G18_000722"
     CDS             complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="R8G18_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HEE9794508.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="R8G18_000723"
     CDS             complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="R8G18_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEE9794509.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="R8G18_000724"
     CDS             complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="R8G18_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEE9794510.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="R8G18_000725"
     CDS             complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="R8G18_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEE9794511.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="R8G18_000726"
     CDS             complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="R8G18_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEE9794512.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            18691..19287
                     /gene="rsmD"
                     /locus_tag="R8G18_000727"
     CDS             18691..19287
                     /gene="rsmD"
                     /locus_tag="R8G18_000727"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HEE9794513.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            19277..19546
                     /locus_tag="R8G18_000728"
     CDS             19277..19546
                     /locus_tag="R8G18_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HEE9794514.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19549..19908)
                     /locus_tag="R8G18_000729"
     CDS             complement(19549..19908)
                     /locus_tag="R8G18_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HEE9794515.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            20049..20675
                     /locus_tag="R8G18_000730"
     CDS             20049..20675
                     /locus_tag="R8G18_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEE9794516.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            20749..22947
                     /gene="zntA"
                     /locus_tag="R8G18_000731"
     CDS             20749..22947
                     /gene="zntA"
                     /locus_tag="R8G18_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HEE9794517.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="R8G18_000732"
     CDS             complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="R8G18_000732"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HEE9794518.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            23515..24180
                     /locus_tag="R8G18_000733"
     CDS             23515..24180
                     /locus_tag="R8G18_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEE9794519.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            24253..24810
                     /gene="dcrB"
                     /locus_tag="R8G18_000734"
     CDS             24253..24810
                     /gene="dcrB"
                     /locus_tag="R8G18_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HEE9794520.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(24814..26031)
                     /locus_tag="R8G18_000735"
     CDS             complement(24814..26031)
                     /locus_tag="R8G18_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9794521.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            26163..27212
                     /locus_tag="R8G18_000736"
     CDS             26163..27212
                     /locus_tag="R8G18_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEE9794522.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            27267..27854
                     /gene="acpT"
                     /locus_tag="R8G18_000737"
     CDS             27267..27854
                     /gene="acpT"
                     /locus_tag="R8G18_000737"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HEE9794523.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            27965..29539
                     /gene="nikA"
                     /locus_tag="R8G18_000738"
     CDS             27965..29539
                     /gene="nikA"
                     /locus_tag="R8G18_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEE9794524.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            29539..30483
                     /gene="nikB"
                     /locus_tag="R8G18_000739"
     CDS             29539..30483
                     /gene="nikB"
                     /locus_tag="R8G18_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HEE9794525.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            30480..31313
                     /gene="nikC"
                     /locus_tag="R8G18_000740"
     CDS             30480..31313
                     /gene="nikC"
                     /locus_tag="R8G18_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HEE9794526.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            31313..32077
                     /gene="nikD"
                     /locus_tag="R8G18_000741"
     CDS             31313..32077
                     /gene="nikD"
                     /locus_tag="R8G18_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HEE9794527.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            32074..32880
                     /gene="nikE"
                     /locus_tag="R8G18_000742"
     CDS             32074..32880
                     /gene="nikE"
                     /locus_tag="R8G18_000742"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HEE9794528.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            32886..33287
                     /gene="nikR"
                     /locus_tag="R8G18_000743"
     CDS             32886..33287
                     /gene="nikR"
                     /locus_tag="R8G18_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEE9794529.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(33407..33766)
                     /locus_tag="R8G18_000744"
     CDS             complement(33407..33766)
                     /locus_tag="R8G18_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEE9794530.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(33763..34036)
                     /locus_tag="R8G18_000745"
                     /pseudo
     CDS             complement(33763..34036)
                     /locus_tag="R8G18_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(34109..35233)
                     /locus_tag="R8G18_000746"
     CDS             complement(34109..35233)
                     /locus_tag="R8G18_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9794531.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(35233..37968)
                     /gene="rbbA"
                     /locus_tag="R8G18_000747"
     CDS             complement(35233..37968)
                     /gene="rbbA"
                     /locus_tag="R8G18_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HEE9794532.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(37965..39032)
                     /locus_tag="R8G18_000748"
     CDS             complement(37965..39032)
                     /locus_tag="R8G18_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEE9794533.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(39398..41020)
                     /locus_tag="R8G18_000749"
     CDS             complement(39398..41020)
                     /locus_tag="R8G18_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HEE9794534.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(41282..42889)
                     /locus_tag="R8G18_000750"
     CDS             complement(41282..42889)
                     /locus_tag="R8G18_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HEE9794535.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            43274..44326
                     /locus_tag="R8G18_000751"
     CDS             43274..44326
                     /locus_tag="R8G18_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HEE9794536.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(44642..45844)
                     /locus_tag="R8G18_000752"
     CDS             complement(44642..45844)
                     /locus_tag="R8G18_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEE9794537.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            46076..47575
                     /gene="pitA"
                     /locus_tag="R8G18_000753"
     CDS             46076..47575
                     /gene="pitA"
                     /locus_tag="R8G18_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HEE9794538.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(47819..48154)
                     /gene="uspB"
                     /locus_tag="R8G18_000754"
     CDS             complement(47819..48154)
                     /gene="uspB"
                     /locus_tag="R8G18_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HEE9794539.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            48545..48979
                     /gene="uspA"
                     /locus_tag="R8G18_000755"
     CDS             48545..48979
                     /gene="uspA"
                     /locus_tag="R8G18_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEE9794540.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            49297..50766
                     /gene="dtpB"
                     /locus_tag="R8G18_000756"
     CDS             49297..50766
                     /gene="dtpB"
                     /locus_tag="R8G18_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HEE9794541.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(50815..51567)
                     /gene="rsmJ"
                     /locus_tag="R8G18_000757"
     CDS             complement(50815..51567)
                     /gene="rsmJ"
                     /locus_tag="R8G18_000757"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HEE9794542.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(51575..53617)
                     /gene="prlC"
                     /locus_tag="R8G18_000758"
     CDS             complement(51575..53617)
                     /gene="prlC"
                     /locus_tag="R8G18_000758"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HEE9794543.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            53820..54662
                     /gene="rlmJ"
                     /locus_tag="R8G18_000759"
     CDS             53820..54662
                     /gene="rlmJ"
                     /locus_tag="R8G18_000759"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HEE9794544.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            54734..56086
                     /gene="gorA"
                     /locus_tag="R8G18_000760"
     CDS             54734..56086
                     /gene="gorA"
                     /locus_tag="R8G18_000760"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEE9794545.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(56140..56223)
                     /locus_tag="R8G18_000761"
     CDS             complement(56140..56223)
                     /locus_tag="R8G18_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HEE9794546.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            56251..56424
                     /locus_tag="R8G18_000762"
     CDS             56251..56424
                     /locus_tag="R8G18_000762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794547.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            56963..57316
                     /gene="arsR"
                     /locus_tag="R8G18_000763"
     CDS             56963..57316
                     /gene="arsR"
                     /locus_tag="R8G18_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HEE9794548.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            57370..58659
                     /gene="arsB"
                     /locus_tag="R8G18_000764"
     CDS             57370..58659
                     /gene="arsB"
                     /locus_tag="R8G18_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HEE9794549.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            58672..59097
                     /gene="arsC"
                     /locus_tag="R8G18_000765"
     CDS             58672..59097
                     /gene="arsC"
                     /locus_tag="R8G18_000765"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HEE9794550.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(59231..59527)
                     /locus_tag="R8G18_000766"
     CDS             complement(59231..59527)
                     /locus_tag="R8G18_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9794551.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            59634..>59895
                     /locus_tag="R8G18_000767"
     CDS             59634..>59895
                     /locus_tag="R8G18_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEE9794552.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT        14008 a        16149 c        15124 g        14614 t
ORIGIN      
        1 gcacggtggt aataatccga ctcccgccag gactaccggt aaccagccaa gttttaccgt
       61 ctttcaccac aatggttggt gacatcgatg acagcggacg tttattcggc ccgacggcgt
      121 tggcatcacc gcccaccagc ccgtaaatgt tcggtacgcc cggtttggcg gagaaatcat
      181 ccatctgatt attaagcaga ataccgctct cgcccgcgac aatgcccata ccgaaggtgg
      241 tgttcagcgt ataggtcacc gccaccgcgt taccgtcttt atccactaca gagtaatggg
      301 tagtttgatt actctcataa ggcgcaagct tgccagggcg aatctcactg gacggcttcg
      361 ctttgttgat atcaatttga tcggcaatgg atttggcata ggctttattg gtcagtgcct
      421 gccacgggac tttgacaaaa tccgggtcgc caagatattc cgagcggtcg gcgtaggcgt
      481 atttctccgc ttctgccatg atttgcatcg catcggcgct gccaaagccg tatttcttca
      541 tatcgaagtt ttccagaata ttgaggattt gtacgatatg gatcccgccg gaggatggcg
      601 gtggcatgga gtaaacctga tacccgcgat aatcgccgct tatcggagtg cgttcgaccg
      661 ctttataggc cgctaaatct tctttagtga tcaagccacc gtttttctgc atctcctggg
      721 cgatctgttc cgcaatcgtg cctttataga attcgtccgg gccgttttca gcaatcatct
      781 ccaggctctt tgccaggttc gcctgcacca gcgtgtcgcc ctttttcagc ggctcgccct
      841 ctttccagaa gatagcttta ctgttttcgt gattcggcaa cacttcgcta ccgtaggttt
      901 tgagatcgtc agccagcgcg tcgttaacga taaaaccatc gcgtgccagt ttaaacgcgg
      961 gctgcacgac tttgttcagc ggcatggtgc cgtatttatc cagcgccagc gagaaacctg
     1021 ctaccgtacc cggtgtgccg gaagccagat gcgaagtgag tgattttttg ctgtccgggt
     1081 tgccctgatc atcgaggaac atatcgcggg tcgctttggc gggtgccatt tcgcggaaat
     1141 cgatagccgt ggtattgcca tttttcgagc ggattaacat aaaaccacca ccgcccagat
     1201 tccctgcctg cggatgcgtt accgccagcg cgtagcccac cgccacggcg gcatcaacgg
     1261 cattcccgcc ctccttgagg atatccaccc ccacctgagt ggcagtggcg tccacagacg
     1321 ctaccattcc ctgtttcgcg cgtaccgggt ggaagacatc ttcctccaca ccatacgaga
     1381 cgggcggcgc aggaggcgcg gcggcggcgc taaaacaact tcctgagagc agagcagcaa
     1441 tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga ttaaggggca
     1501 accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg gaagcgagta
     1561 aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat tcttacggca
     1621 ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc taaccagccg
     1681 gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca gcaaatccag
     1741 caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca gcagcattta
     1801 gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg ggagcgaaat
     1861 cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag caaccgtaat
     1921 ccggatagtt cgttgaatca gcagcatatg ctgccggaaa ggagaaacgg cgacatgctg
     1981 aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc gtaaagttcg
     2041 gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc agcaactctg
     2101 ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg tctttcaact
     2161 gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg cagcccagcc
     2221 gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca cgcggcagtt
     2281 ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac agcagcggcg
     2341 gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc gttaatggtc
     2401 cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg cgacagcgtt
     2461 ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg taccaactgc
     2521 ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc cagccgttgc
     2581 tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc gataacttcg
     2641 cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc gccagggtgt
     2701 tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa ggccagttac
     2761 tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg ccaaatgcag
     2821 ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca ccagcttctg
     2881 ctcgccccag cgtccgtgcg ccaggttatc tgcgccgagg atctccagcg tgtccatcac
     2941 cagcggcaca ccaccttctg cctgcgagct tagcgctata tgttccgggc gaatgccgag
     3001 agtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc
     3061 gtccagttcg aaatgcgtgc cttcattatt cacgcggcct gtcagcaggt tcatcgccgg
     3121 gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt caactggtgt
     3181 gccaatctgt tcggcaacgc caccgttcat caccattact cgctgggcga gcgtcatcgc
     3241 ttcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcaactgttg
     3301 cagttcaaga cgcatctgca cgcgcagctt ggcatcgagg ttagagagcg gctcatcaaa
     3361 caagaacacc gccggatcgc gcacaatcgc gcggcccatc gccacacgct ggcgctgacc
     3421 gccggatagc tcacgcggac gacgcttaag cagaccgtcc agttccagaa tgcgcgccgc
     3481 ttctttaacg cgctcggcaa tttgctgctt gcccatgccg cgaattttca gcccccacgc
     3541 catgttctcc tctacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat
     3601 cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc
     3661 tgtcacccgc tccagcccgg caaccatgcg cagcagcgtc gatttcccgc agccagacgg
     3721 cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgacctg
     3781 ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct
     3841 cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc accaccggag
     3901 ggataagcgt taacaacatc gccgccatta ccgagttcca ttccgtggtg ccttcgcctg
     3961 tagcgatcat ccctttgatc cctgccacgg tggtgccgag atcaacgtcg gtaataatca
     4021 acaacggcca caaatactga ttccagccgt agataaaggt gatcacaaac agcgccgcca
     4081 gattagtttt ggacaacgga aaaacgatgt cgcaaaagaa acgcattggt gacgcgccgt
     4141 cgatccgcgc ggcttccacc aattcatccg gtagggtcat aaagaactgg cggaacagaa
     4201 acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcattt
     4261 tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg
     4321 tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa
     4381 atgccgagag catcgagacg gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc
     4441 tgttaagcaa catccgccag aacggcgcgc tattcgtgcc taccccgtta acccagatgt
     4501 tgtggatatt ttccagcaga tgtgtgccgg ggatgagcgt catcggcgcg gcatagacgg
     4561 cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga
     4621 tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc tcaatcattg
     4681 gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa
     4741 catcaacacc accgactgcg cggcagacga agccagatcc agtccggtaa agccttcgcg
     4801 gtagatctta taaatcagcg tagtggtggc ctgaaccggc ccgccggacg tggcggcgtc
     4861 gatcaccggg aaggtgtcga agaaggcgta caccaggttc accaccagca ggaagaaact
     4921 caccggagcg ataagcggca gagcaatctt aaagaagcgg cgaatcggcc ctgcgccgtc
     4981 aatagctgcg gcttcaatca acgaacgggg aatggagtgc agcgcggcat agaagaacag
     5041 aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca ttgcctgacc
     5101 gctgttttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc
     5161 gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata
     5221 cggcagcagc atcaaggttt gatagaaacg gctgccgcgc acgatgtact ccaccagcgc
     5281 cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt
     5341 cgtccagaag gagtctagat agtagctgtc atggaacagc gtgacgaagt tatccagacc
     5401 gacaaactgg ctggagaaac caaacggatc gacgctttgc cgcgagtacc acaacgcttc
     5461 gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata
     5521 aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac
     5581 tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac
     5641 gcatccgaca ttacactgat taagacttcg tcgatttctc aaagcggcgc agcaactgat
     5701 ttccacgctc aacggcggta tccagtgcct gctgtggtgt cttcttaccg gtccacacgc
     5761 tctccagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgcagccctt
     5821 tggtgaacgg caacggcggc ttattcagca tctgacgcgt cgcggtatcc gcccctgggt
     5881 ttttctcata aaagccctgc tcacgggtca ggtcatacgc tgctttggtg attggcagat
     5941 aaccggtttt ctgatgccac tcggcagcgt tttctggctt cgcgaggaag tcgaggaact
     6001 tcgccacgcc ggtatacgtt tctttatttt tgccctgcat cacccacagg ctggccccgc
     6061 cgataatggc gttttgcggt gcatctttcg catcggcgtc gtaaggcatc atgcctacgc
     6121 catagttaaa tttggcgtac tcacgaatat tagcgagaga accggaagag gcggtggtca
     6181 tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga
     6241 agtcgccctt cttgttcatc tcttcgagca tggcaatgtg tttcacctgc tccggcttgt
     6301 tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac
     6361 cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc
     6421 acttcatacc ggaggctttc agttttgcgg aagagtccgc cagatcctgc caggttttcg
     6481 gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg
     6541 gagtcgagct gttgaatggc tgggagagta agtggcccgt tttgctgtcg gagtagtaac
     6601 cggaaaccgt cggcacaaac tgcgactcat cgaattgaat ccctgcctct ttaaacacgt
     6661 catataccgg tttaatggct ttcgatgcca tcatggtggc ggtgccaact tcataaacct
     6721 gcaagtaagg tgtcagaaag atgacaaggc ggtgacggcg tgggtgaggg aaaatgggag
     6781 atggggcacg gataagcgga aaaatagaag gtctgaatca aactctacag attactcatc
     6841 gtttcatgcc ggatgcggcg taaacgcctt atccggccta caagatcgtg caaattcaat
     6901 atattgcaac tcacccagta ggcctgataa gcgcagcgca tcaggcaatt ttacatttgt
     6961 cacctgtctc aaaggagtct tttgactcct tatcaatcaa cgtgttatta cccgcctaaa
     7021 tacgcacttc tcaccgcttc attcgccagt agtgcatcac cagtatcgga gagcaccaca
     7081 tggccgtttt ccagcacgta gccgcgatcc gccagcttta gcgcctggtt ggcgttctgc
     7141 tcgacgagga agatagtcat cccctgctcg cgcagctgct cgatggtgtc gaaaatttgc
     7201 tggatgataa tcggcgcaag accaagcgat ggctcatcaa gtagcagcaa acgcgggttg
     7261 ctcatcagcg cgcgaccaat cgccagcatt tgttgctcac cgccggacat ggtgcctgcc
     7321 cgctgaacac ggcgctcatg cagacgtggg aacagttcat acacccactt tatgcgctcc
     7381 tggaactggt cgcgttcggc aaaaaagccg cccatcgcca ggttctcttc caccgtcatc
     7441 cgcgagaaga cgcgacgccc ttccgggaca atcgctaccg cttcgcgcat gattttcgcc
     7501 gtctgccagt cggtaatgtc tttatcatca aacacaattc gcccgctggt agcgcgcgga
     7561 tcgccgcata acgtgccgag caaggtggtt ttccccgccc cgttcgcgcc aatgagggtg
     7621 acaatctcgc cctgattgat atgcaggctc acctcatgca gcgcctggat tttgccgtag
     7681 tgggcgctga ctttgtcaaa ggacaacatg actttttcca tcttatgcct cacctaaata
     7741 ggcacggatc acgtccgggt tattacggat ttgctccggc gtaccgtttg ccagtggcgt
     7801 cccctgattg accacataaa ttcggtctga aatccccatc accagcttca tatcgtgttc
     7861 aatcaacaag atagtggtgt tgtgatgatt gcgcagttcg gcaatcagct catccagctc
     7921 tttggtttct tttgggttaa gacctgccgc cggttcgtcg agcattaaaa tctccggctg
     7981 cgtcaccatg cagcgggcaa tctccagacg gcgttggtca ccataggcca ggttactcgc
     8041 ctgacggttg gcgtgttcca gcaaaccaat gcgctcaagc caggtggcgg cgcggtcgag
     8101 cgcttcgctc tgggcgcgac ggaaagaagg cgttttcaac aggccagaga acagcccggt
     8161 tttcagttgt tgatgctgcg ccaccagcag gttttcaatt accgtcattt cacggaacag
     8221 acgcacatgc tggaaggtgc gcaccacgcc catgcgggca atttgctgcc ccggtaaacc
     8281 ttccaggtgc tgattgcgca gtaaaatggt gccgccggtg ggtttgtaga atccggtcag
     8341 acagttaaaa accgtagttt ttccggcacc gttagggccg attaacgaga cgatctcctg
     8401 cgggtacagt tcaagattga cgttgttcac cgccagcagg ccgccgaagc gcatcatcag
     8461 gccgttcaca gataataatg gctgactcat gcctgctctc ctttcgctgc gccgtttttc
     8521 agcttcagtt gcgggcgcgt catgggcagc aagccctgcg gacgccagat catcatcagc
     8581 accatcaaac caccgagcat taacatgctg tattcgttga aatcacgcat caattcgcgc
     8641 gataccacca gcaaaattgc cgccagaatc accgcaaact gcgatcccat accgccgagc
     8701 accactatcg ccagtacaaa cgccgattca gcaaaggtga aggattccgg gctgacaaag
     8761 ccctggcgcg cagcaaacag cgttccggca aaaccggcaa acgcggcact gatggtaaag
     8821 gcagtcagct tgatacgacg cgggcttaag cccagcgaac ggcaggcgat ctcatcttca
     8881 cgcaacgctt cccacgcacg ccccagcggc atccgcagca ggcggttaat gacaaacagg
     8941 cttagcacca ccagcagcaa cgccaccaga tacaggaaga tgacgcgatc ggagggatcg
     9001 tatttcaggc caaagaaatt actgaacgtg tcccaaccgc cttcacgggc ggtacggctg
     9061 aactcaaggc cgaagaaagt cggtttcggg atctgactga taccgttcgg gccgccggta
     9121 atttcggtgt tattgagcag caatatgcgc acaatttcgc cgaaaccgag ggtaacgatc
     9181 gccagataat caccgcgcag acgcaacacc ggaaaaccaa gcaggaagcc cgccgccgct
     9241 gccatcagtc cggcaatcgg caaacaggtc cagaacccca gaccgtaata gtgattgagc
     9301 agcgcaaaag tgtaagcgcc gatggcgtaa aaaccgccgt accccagcac cagcagacca
     9361 gaaagaccaa caaccacatt cagcccgaga ccgaggataa tgtagatcat ggtcagagtg
     9421 gcgatatcca ccgtcccacg tgacaccata aacggccacg acaccgcaag caccagcagc
     9481 gccacgagga acagtttctg cttcaccgtg gagccatcaa tcgcgggcag aataaacttc
     9541 ggcccggaaa cgctttttag ccctttctgg aaagcaggtc gcaaaagctg gaagaaaaag
     9601 accaccgccg tgccgataaa cacccactgc caacggacat ccgaagcgct gtcgaccacc
     9661 agtttggtgc catccagctc cagttgcacg cccataaaga cgccagcgag gacaaagaac
     9721 atcgcggcag agagcagtgc cattgcaata tgcatcggtt tcatactttc tctacctccg
     9781 ggcgacccag aataccggtc ggcatcacca gcagcaccag aatcagcagg gcgaatgaga
     9841 ccacatcttt atattccgta ctcagatagg cagaagagag cgcctccgca atccccagga
     9901 tcagcccgcc aatcatcgcc cctggaatgc tgccaatccc accgagcacc gcagcggtaa
     9961 aggctttcat cccggccata aagccgatat aggggttaat gacgccgtag aactgaccga
    10021 gcagtacgcc agccactgcc gccatcgccg cgccaataac aaaggtcaaa gcaatcaccc
    10081 ggtcggtgtt gatgccaagc aggctcgcca ttttcagatc ttccgcgcag gcacggcagg
    10141 cgcgccccat acgggaataa cgaatgaaaa tcgtcagcgc cagcatggcg aggaaagtaa
    10201 caatccagat cactgcctgc atggtggtaa tagaggcaga gaagttttcg ctatgcccca
    10261 ccacccactg accgttaaac agactgggca gcgccacgtc acgcgaacct tcggtcaggc
    10321 taacgtagtt ttgcaggaag atggacatgc cgattgccga gatgagcgca atcaggcgct
    10381 tagagttacg caccgggcga taggctaccc gttcgatact ccagccgtag gcgctggcaa
    10441 tgacgattgc gccgacgaat cccgccgcca ccagcagcca gccggtatca atgcccatca
    10501 tcatcagtgc ggcgatgatc ataaaggaga catagctacc aatcatgtaa acctcgccgt
    10561 gggcgaagtt gatcatgccg ataatgccgt aaaccatggt gtagccgatg gctatcagcg
    10621 cgtaggtact gcccagcgtg acgccgttaa acatctgctg caagaaatat aaaaactgct
    10681 cagacataag gtaacctttc taaacccgcc cgcatttcac gggcggtggg atgatcactt
    10741 ggctgccgtg gatgaaccat cggcgtgcca ctggaagaca ccaaaatcaa atcccttaag
    10801 atcgcctttt tcatcccagt tcagcggccc aatcacggtg tttgcaccgt tagcttttaa
    10861 atctttcacc agcgccagcg gctcatcgct gccagtacgc tcaagggcag tcgccagaga
    10921 ttgcaccgcc gcgtaggtga tccagacata aggcccggac ggatctttct tgtctgcttt
    10981 cagcgcatca acgatgccct ggtttgccgg atcctggtca tagcgttttg gcatagtgac
    11041 caacatgcct tcggcagcat cgccagcaat attcgacaat gatgcgttac ccacaccttc
    11101 cggccccata aactgagttt tcaggccaac ggaacgggcc tggcgcagca tctgccccat
    11161 ttccgggtag taaccgccgt agtaaacgaa gtcgatgttt tcttttttca ggcgggcgat
    11221 cagcgcggag aaatctttct ccccggcggt aataccgtcg aagaagacga cgttggcgtt
    11281 agccgctttc agcccgtcct gcaccgaacg cgccagccct tcgccatact gttgtttgtc
    11341 gtgaatgatg gcgatgcgct ggggcttcac cgtctcaaga atgtattttg ccgccgttgg
    11401 cccctgggaa gagtccagcc cggcagtacg cataatgtgt tgataaccgc gttgggtcag
    11461 ctccgggttg gtcgctcccg gcgagatcat cagaatacct tcgtcttcgt agatatctga
    11521 tgcaggttgg gtagaagaag aacacagatg accaataacg tatttaatgc cgtcattaac
    11581 gattttgttg gcgaccgcaa cggcttgttt cgggtcgcag gcgtcgtcat attccacgcc
    11641 aaccagcttg tcgcccttaa ttcccccttt ggcattaatg tctttaatcg cctgacgcgc
    11701 gccgttaaat tccatatcgc cccactgggc aatcgggccg gacatcgcgc cgacaacggc
    11761 gactttaata tcgtcagcca tagcggtgtg tgaaattgcc agtgcaatca tccctgcgat
    11821 gatagttttc gcattccgtt tcatagtcaa aaatccccat tcgtgatgtt gtgttgcttt
    11881 gtttttatgt gttaacaaat cagactgttc tttttttata ctgcactgtt tttgcctgtc
    11941 tgattaaggg tttagcgcag tattttgtga taacagtgat taaaatccct atttttcagt
    12001 cgattaagaa cagataatat tctgaattta ttgatagata aacagaaaaa agtgcctttg
    12061 tcagcataaa ataacggcac aaagggcgga ataattcact accattcagg ggattatgct
    12121 gggtattttt cattctctaa tgttttaact ttgtaattat tgctgttaaa aaattaatca
    12181 cctgccaaaa gaaataaaaa agagaaagcc tccgattaaa ttatttcgct acactggttc
    12241 cacttttgtg atttacacgg gttacccatg aagctgacca tcattcgatt agaaaacttt
    12301 agcgaccagg accggattga cctgcaaaag atctggccgg agtattcccc ttcctcttta
    12361 caggttgacg ataaccaccg tatctacgcc gcgcgtttta acgagcgcct gctcgctgcc
    12421 gtacgggtaa ccttaagcgg caccgaggga gcactggatt ccctgcgtgt gcgggaagtc
    12481 acccgacgtc gcggtgtggg gcaatatctg ctggaagagg ttttgcgtaa caatcctggc
    12541 gtttcatgct ggtggatggc ggatgcaggc gtggaagatc gcggtgtgat gacagcgttt
    12601 atgcaggcac tgggatttac ggcgcaacag ggcggctggg agaagcgtta atcgtcaagt
    12661 tgatttcaaa agtgatgacc aacgagaaat tgcctgatgc gctacgctta tcaggcctac
    12721 aatgtgtgtt gcaatttact gatttctttg gatcttatag gccggataag gcgtttacgc
    12781 cgcatccggc atgaagcaac gtactcgata ttagcaattt ggcggcaacc caaagttgcc
    12841 gattaatgat tacttcgcat ctgtcgccgt gccgttggcg tgccagtcaa atacgccgaa
    12901 ctcaaagcct ttcagatcgc ctttctcatc ccacgtcagc ggtcccatta cggtatccac
    12961 ggagttcgct ttcaggtatt tggcgatttc agccggatcg tcagactgat tcaggcccgc
    13021 ctgcaaagat tgcagcgcgg cgtaggtggt ccaaacgaat gcgccgcttg ggtcctgttt
    13081 tttcgctttg atcgcgtcaa caatgggttt gtttgccgga acctgatcgt agttcttcgg
    13141 cttggtcacc agcagccctt ccgctgattc gcccgcaatg ttagacagcg aaacgttagc
    13201 cacaccttcc ggccccataa actgagtttt cagccctgcc gcgcgtgcct gacgcaggat
    13261 ttgccccatt tccgggtggt aaccgccgta gtaaacgaag tcgatattct ctttcttcag
    13321 acgcgccacc agcgtagaga aatctttttc cccggcggtg ataccatcaa agaacaccac
    13381 gttagcattg cctttcttca ggccgtcctg caccgctcgt gccagacctt cgccgtattg
    13441 ctgtttgtcg tgaacgatag caatacgctg cggtttcact ttctcaagaa tatatttcgc
    13501 cgccgtcggc ccctggtcgg agtccaggcc ggtggtgcgc aggatcagct gatagccacg
    13561 ggcggtcagc tccggcgcgg ttgccgctgg ggtgatcatt aaaatgcctt cgtcttcgta
    13621 gatgtcagac gcaggctgcg ttgatgaaga acagaggtga ccaatcacat atttaatgcc
    13681 gtcgttaacg actttgttcg ccaccgcaac cgcctgtttc gggtcacagg catcgtcata
    13741 ttttacgatt tgcagtttgt tgcctttaat gccgccttta gcgttgatat ccgcaaccgc
    13801 ctgctctgcg ccggtaaact cttggtcacc gtactgcgca accggaccgg acattgcgcc
    13861 cacgaccgcg actttaatat cttctgccag agccatattg ctgaatgcca gcgcgataca
    13921 tcctgccagt aacgctttac cctttatgtt catcctgaga atccccattc ttctggttat
    13981 tacgtgtgtt gtgatgttgt tgttcagcac tttatttcgt tttatgcatg gctacccgtg
    14041 ctttagcagc atactctgct aaaacatacc cgatttttat gatattggaa tagctatttt
    14101 gacagtttat taacaatctg cgtggggatt ggcgttttgc cggaggggaa attgattatt
    14161 gtagaggccc aaaaaacaaa aaccccggac tcgcatccag ggttctctgc ttaatagcgg
    14221 aaattacgct tctatagcag cgcgcagttt tttcatcgcg ttcttttcca gctggcgtac
    14281 acgttcagcg gaaacaccgt aacggtcagc cagttcctgc aacgtggact tgttgtcttc
    14341 gtccagccag cgcgcgcgga tgatgtcctg gctgcgttcg tccagacctt gcatcgcgtc
    14401 ggtcagacgg tttgccgcct gctcttccca gttatcatct tcgatgccgt cggcaaagtt
    14461 agatgattta tcctgcagat agagcaccgg tgccatcggc tggctgtcgg aatcgtcgtc
    14521 ggaagacagg tcaaaggtca tgtcctgtgc cgccatacgt gattccatct cacgaacgtc
    14581 tttgctggtt acgcccagtt cacgggccac catttcgact tcatcctggt taaaccagcc
    14641 cagacgctgc ttggttttac gcaggttgaa gaacagtttg cgctgcgctt tggtggtcgc
    14701 aactttgacg atacgccagt tacgcagaac gtattcgtgg atctctgctt tgatccagtg
    14761 aacggcgaag gagaccaggc gcacacccac ttccgggttg aaacggcgca ctgctttcat
    14821 caggccgatg ttaccttcct gaatcaaatc cgcctgtggc aggccatagc ccgcataatt
    14881 acgagcaata tgaacaacaa accgcaggtg agacaggatc agcgttttag ctgcttccag
    14941 atcgccatgg taatgcagct tttcagccag cgcccgctcc tcgtcagccg acaacatcgg
    15001 ccacgcgtta gctgcccgga tgtaggaatc caggttgcca actggggcta aagctaaact
    15061 ttgcattttg tcagtcattc aaatcctctc aatcgatatc ttctggcgct tcagtggtag
    15121 caacaactgt gccagagctt aagagcaacg aggttatcat ccactgtttt atcagacagt
    15181 gattttatcc acaagttcaa tgcaagcttg tgaataaatt acgcacaaaa tgtgacatag
    15241 agatgaaata ccgggaagag acaacgggat ctctttccct gctacggaac ccattgcagg
    15301 gaaagagtat aacacgcttt tattattcag gcgtaaagtg gcgtaaatgt tgtaccgtgg
    15361 caagccacgc tgccacccag ccaatcatcg agcataccag cagcaatagc aggcattcat
    15421 cgaacgataa accattgata tcaaacttcg ttccgaaaac ctgtgccact tccgcaaccg
    15481 ccgatgacaa tcgcagcacc agaatttctg acaaaattaa tgacaacaat gcgccagaaa
    15541 atcccagcag tgcgccacca tacaggaacg ggcgaaggat gaaaccatcc gtcgcaccaa
    15601 tcagtttctg cacgttgatg gagtcacggc gagcaaagat gctcagacgt acgctgttgc
    15661 cgatgacgag gaacactgcc gccaccatca acacgccgat catcgccgaa acgcgcccga
    15721 ccagcccggt caacgccgcc agacgggcaa accagctgtc atccatccgc acttcgtcaa
    15781 tgccgttaat ctgcgtgata cgatcacgca gcgtattgag tgattccgtc ccctgaaaat
    15841 cgagtttcgg gatcaccacc gccactgccg gaagcgggtt ttcttccagc atatccagcg
    15901 caccaccaaa accagaccag ttacggaact cgcccagtgc gtcttcacga gaaagatagt
    15961 tcactttctc cacgccttgc tcggcctgca actgtgccac cacgcccgca gcggcgtcat
    16021 cgtccagcgt tttttgcaag taaacagtga tttgcggtga cggataatac tgcgtcgccg
    16081 cctgattaac gtttttgtac accatataac agacgctggg cagcgtcagg gagatggcga
    16141 taaccatcac cgttaaaaac gtggcgaacg gtttgctttt cagatcctgc aatgcgccgt
    16201 ggaaggcata gcgcacctgt tcgttgaaaa cgttggtttt gcgatttacc ggttttggcg
    16261 aggatttcgc gcgtttcggt gcgttacgac cgccgtcgcc tgagccgccg accgatttac
    16321 ggaagcgatc cagacgccca ccaaactgcc gaatatgatt gattgcatcg cgcttattca
    16381 tggcccacgc ctccatgcaa gtgaccatcg ctcagggtga gcatgcgatg ggaacgccgt
    16441 gaaatcaggt tgatgtcgtg cgttgccatc aatacggtta ccccaacgcg gttaaactct
    16501 tcaaacagac gtagaatccc ttccgacaac gcgtcgtcca ggttaccggt cggttcgtcc
    16561 gccagcagta ccgcgggctt attcaccacc gcgcgggcaa tgccaacacg ctgttgttca
    16621 ccgccggaaa gctgaatagg gaagttcttc gctttgtcca gtagcccgac tttatccagt
    16681 gccgccgaca cccggcgacg aatatcgtca ccgctggcac cagcgataat cagcgggatc
    16741 gccacgttat cgtagacagt acggtccatc agtagatggt gatcctggaa aatcatgcca
    16801 atctggcggc gcagaaacgg aacttcacgg tttttcagac gcgtgatgtc atggccgcta
    16861 aaccagattt tcccggcgct gggccgctca atcccacaga tcagcttcag gagggtactt
    16921 ttccctgcgc cggaatgacc ggtcagaaac gccatctcac ccggctgcat atggaacgta
    16981 acgccctgca gcgcctgtct cccaccgaga taagccttgc tgacatgttc aaagcgaatc
    17041 attgttaatc ctctcgggca aaaagtgcct ctataaagtc gtccgcctta aacggacgca
    17101 aatcctcaat acgttcgccg acaccaatgt agcggatagg gataccaaac tggtcagcca
    17161 ccgagaaaat taccccgcct ttcgccgtgc cgtccagttt cgttagcgtg atgccggtta
    17221 agccaacagc ttcgtggaac agtttggcct ggcttaccgc gttctgcccg gtgctggcat
    17281 caatagtcag cataacttca tgcggcgctt caacgtcgag tttcttcatc acgcggacga
    17341 ttttcttcaa ctcttccatc aggtgcgatt tgttctgcag gcgtccggcg gtatcggcaa
    17401 tcaggacgtc gatattacgc gctttagctg cctgaatggc gtcgaagata acagaggcgg
    17461 aatccgcccc ggtatgctgg gcaatcaccg gaatattgtt gcgctgaccc cagacctgaa
    17521 gctgttcaac cgctgctgca cggaaagtat cgcccgccgc cagcatcacc gatttaccct
    17581 gctgctcaaa ctgacgtgcc agcttaccaa tcgtcgtggt tttacccaca ccgttgacgc
    17641 ccaccatcag gatcacgaac ggcgttttac cttcaacatt cagcggctca tcgactttcg
    17701 ccagaatctc acccatctct tctttcagca ggccatagag cgcctcggca tcacgaagct
    17761 gcttgcggga tgcgccttcc gtcagattgg tgataatttt acgtgtggtt tccacgccca
    17821 catcggcgat caaaagctgc tcttccagct cctcaaacag atcatcgtcg atttttttac
    17881 cgcggaacag gctgataaat ccggaaccga gattttcttt ggtttttaac aggctgcgtt
    17941 tcaggcgcgc gaagaaacct tctttggttg gtttttcctg ctcctgagcg atttcttcca
    18001 ccggctgctc ttcttctgcc ggaggaacca ccatcaccgc ctcttctgct gcttcggctg
    18061 ccagcgcctg tgcttccagc tcttcgtcgg tgatttcttc tttagccgct tcttcttccg
    18121 ccgctttgac aatctctacg gtttccgctt cagcctgcca ctcttctggc gaaacctctt
    18181 cggcgttgac gtcttccggc aacggcagct cttcacgttc gatagccact ggctccggcg
    18241 tttcttctac gaccggttcc ggctgtgcaa caacttccgc ttcaggctgc gctttttcac
    18301 tttcaacaac ctgttcagtg acttccacaa cgtcggcagc aaaagtttcc gcctcggctt
    18361 cagtatgcgc ctgcggctgc tcttcaacgg cttgttccga agccttcaca ggctcttgcg
    18421 cctgaacgat ttcttctaca accggttgtt cattctgaac ttctgtctct ttttccgggg
    18481 tctgctcttt ttgaccaaag cccagccagg aaaaaaagcc acgttttttt tctttcgcca
    18541 tttgcgacta cactcctcgc tgttgcttca tggcacagcg ttaacgctat gtacatagca
    18601 gctaaaaaaa tgatgaaata gtctatcact taacttaatt cacatcaccg cctgcaagta
    18661 tgtgttatct ggcggattga gcaatttatc atgaaaaaac cgaatcattc cggcagcggc
    18721 caaatccgca ttattggcgg gcagtggcga ggccgtaaac tcccggttcc tgatagccca
    18781 ggtctgcgcc ccaccaccga ccgcgtacgc gaaacgttgt ttaactggct ggctccggtc
    18841 attgttgacg cccaatgtct ggattgcttc gccgggagcg gcgcgctggg gctggaagcg
    18901 ttatcgcgct acgctgcggg ggccacgtta attgagatgg atcgtgcagt ttctcagcag
    18961 ttaattaaga atctggcgac actaaaagca ggcaatgcac gcgtggtgaa cagcaacgcg
    19021 atgtcattcc tggcgcaaaa aggcacaccg cataatatcg tgtttgtcga tccaccgttc
    19081 cgccgtggct tgttagaaga gacgataaat ttactggaag ataacggctg gctggctgac
    19141 gaagccctga tttatgtcga aagcgaagtc gaaaacggtc tgcccaccgt tccggcaaac
    19201 tggtcgttac atcgggaaaa agtggcgggt caggtggctt atcggctgta tcaacgcgaa
    19261 gcacaaggag aaagtgatgc tgattaatat tggtcgtttg ttaatgctct gcgtttgggg
    19321 atttttaatc ctcaatctgg tgcatccctt cccgcgcccg ctaaatattt tcgttaacgt
    19381 ggcgctgatt tttaccgtgc tgatgcatgg tatgcagctg gcgctattga aatccacttt
    19441 accgaaagat ggcccgcaga tgaccaccgc cgaaaaggta cggattttcc ttttcggcgt
    19501 gtttgaactg ctggtctggc agaagaaatt taaagttaaa aaataacctt attgttcgcc
    19561 aacaaagcta acaaagcgcg ttcctttata gctcagcgta cctttatcgc ccactgtcag
    19621 ggcgtggtac tgctgggcgt cgaggcgaaa cgtctgctcc attcctccgc tttgcggttt
    19681 gaagcttgcc tcgtagcgca tactggtgcc tgccggagtc acttcctgct ggcgcgaacg
    19741 gcgatcgtta atcggttttt cccgcttgtt gctcaccacc accagctttt gctggagcgg
    19801 agccatatca ttatcagctt tttcccgtcg ctgttgcata aaacgaaacg atgcggcgac
    19861 gacaattaag ccaatgataa caataaagaa aagaggtggt ttgctcatct ttatccctca
    19921 tcggaaaatg cggaaataag cataccctgc cagttatggt gttgtcatcc gtccaccctc
    19981 gccactaaac tggaagcaag accgtaggca ttccgcttac gaaaaaataa cgaattcaag
    20041 gaactaagat gctttggtcg tttatcgctg tctgtctttc cgcatggcta tctgtggatg
    20101 catcgtatcg tgggccaacc tggcaacgct gggtgtttaa accgttaacc cttcttctcc
    20161 tgctgttact ggcctggcaa gcgccgatgt tcgacgccat tagctatctg gtgctggcag
    20221 ggctgtgcgc ctcactgctg ggcgatgcgc taaccctgtt gccacgtcaa cgtctgatgt
    20281 acgccatcgg cgcgtttttc ctctcgcacc tgctgtacac catctatttc gccagtcaga
    20341 tgacgctctc tttcttctgg cctctgccac tggtgttgct ggttctgggt gcgctgttac
    20401 tggcgattat ctggacgcgg ctggaagagt atcgttggcc tatctgcacg tttatcggca
    20461 tgacgctggt gatggtgtgg ctggcaggtg aactgtggtt cttccgtccg accgctccgg
    20521 cgctctctgc gtttgttggc gcttcgttgc tgtttatcag taactttgtc tggctgggga
    20581 gccactatcg ccgacgcttc cgtgcggata acgcgattgc tgcggcctgc tactttgccg
    20641 gtcacttcct catcatccgc tcactgtatc tctgataaaa cttgactctg gagtcgactc
    20701 cagagtgtat ccttcggtta atgagaaaaa acttaaccgg agtatgccat gtcgacacct
    20761 gacaatcacg gcaagaaagc ccctcaattt gctgcgttca aaccgctaac cacggtacag
    20821 aacgccaacg actgttgctg cgacggcgca tgctccagct cgccaacgct ctctgaaaac
    20881 gtctccggca cccgctatag ctggaaagtc agtggcatgg actgcgccgc ctgtgcgcgc
    20941 aaggtagaaa atgccgtgcg ccagcttgca ggtgtgaatc aggtgcaggt gttgttcgcc
    21001 accgaaaaac tggtggttga tgccgacaat gacattcgtg cacaagttga atctgcggtg
    21061 caaaaagcgg gctattccct gcgcgatgag caggccgccg acgagccaca agcatcgcgc
    21121 ctgaaagaga atctgccgct gattacacta atcgtgatga tggcaatcag ctggggtctg
    21181 gagcagttca atcatccgtt cgggcaactg gcgtttatcg cgaccacgct ggttgggctg
    21241 tacccgattg ctcgtcaggc attacggctg atcaaatccg gcagctactt cgccattgaa
    21301 accttaatga gcgtagccgc tattggtgca ctgtttattg gcgcaacggc tgaagctgcg
    21361 atggtgttgc tgctgttttt gattggtgaa cgactggaag gctgggccgc cagccgcgcg
    21421 cgtcagggcg ttagcgcgtt aatggcgctg aaaccagaaa ccgccacgcg cctgcgtaac
    21481 ggtgagcggg aagaggtggc gattaacagc ctgcggcctg gcgatgtgat tgaagtcgcc
    21541 gcaggtgggc gtttgcctgc cgacggtaaa ctgctctcac cgtttgccag ttttgatgaa
    21601 agcgccctga ccggagaatc cattccggtg gagcgcgcga cgggcgataa agttcctgca
    21661 ggagccacca gcgtagaccg tctggtaacg ctggaagtgc tgtcagaacc gggtgccagc
    21721 gccattgacc ggattctgaa actgatcgaa gaagccgaag agcgtcgcgc ccccattgag
    21781 cggtttatcg accgtttcag ccgtatctat acgcccgcga ttatggccgt cgctctgctg
    21841 gtgacgctgg tgccaccgct gctgtttgcc gccagctggc aggagtggat ttataaaggg
    21901 ctgacgctgc tgctgattgg ctgcccgtgt gcgttagtta tctcaacgcc tgcggcgatt
    21961 acctccgggc tggcggcggc agcgcgtcgt ggggcgttga ttaaaggcgg agcggcgctg
    22021 gaacaactag gtcgtgttac tcaggtggcg tttgataaaa ccggtacgct gaccgtcggt
    22081 aaaccgcgcg ttaccgcgat tcatccggca acgggtatta gtgaatctga actgctgaca
    22141 ctggcggcgg cggtcgagca aggcgcgacg catccactgg cgcaagccat cgtacgcgaa
    22201 gcacaggttg ctgaactcgc cattcccacc gccgaatcac agcgggcgct ggtcgggtct
    22261 ggcattgaag cgcaggttaa cggtgagcgc gtattgattt gcgctgccgg gaaacatccc
    22321 gctgatgcat ttgctggttt gattaatgaa ctggaaagcg ccgggcaaac ggttgtgctg
    22381 gtagtacgta atgatgacgt gctgggtatc attgcattgc aggataccct gcgcgccgat
    22441 gctgcaactg ccatcagtga actgaacgcg ctgggggtca aaggagtgat cctcaccggc
    22501 gataatccac gtgcagcggc ggcaatcgcc ggggagctgg ggctggagtt taaagcgggc
    22561 ctattgccgg aagataaagt caaagcggtg accaagctga atcaacatgc gcccctggcg
    22621 atggtcggtg atggtattaa cgacgcgcca gcgatgaaag ctgccgccat tgggattgca
    22681 atgggcagtg gcacagacgt ggcgctggaa accgctgatg cagcattaac ccataaccac
    22741 ctgcgcggcc tggtgcaaat gattgaactg gcacgcgcca ctcacgccaa tatccgccag
    22801 aacatcacca ttgcgctggg gctgaaaggg atcttcctcg tcaccacgct gttagggatg
    22861 accggcctgt ggctggcggt gctggcagat acgggtgcga cggtgctggt gacagcgaat
    22921 gcgttaagat tgttgcgcag gagataaggc aaaccgatcg caacattgag cgcgatcggt
    22981 cccctcgccc ctctggggag agggttaggg tgaggggaaa aggcggcatc gaagccaatc
    23041 tttccccatc aaccgccttt acgaatcaaa taacgataag gcagtccatc cgtctctttc
    23101 gcaaccagtt cgtgttccat aaaggtacaa aacccaggaa tatcgcgggt agtggccgga
    23161 tcgtcggcga taatcagcaa cgtttcgcca ggctgcatat tgcgcacagt tttgcgcacc
    23221 atcattacgg gttccgggca gcgcaggcca agtgcgtcga gtgtgtggtc agggctggaa
    23281 aagagatcgg tcattttctt ctcatcacat aaaaaaacgg cgctagttta cgccctgtga
    23341 gtccgtaagc aaaccaggtt aacgattgcg tgaaaattaa ccattgcatt gtcaacgtaa
    23401 agcagtatca tgcggcggct cgaaaaaagg gtaagcacgt tattatgtta aggtaacaga
    23461 cgtgtcgtac gtattgggtt ccctcacccc aatggttaat caaaaaggta caatatgaac
    23521 gttttctcgc aaactcaacg ctataaggcg ttgttctggt tatcgttatt tcatctgctg
    23581 gtgatcacct ccagtaacta tctggttcag cttcccgtct ccattttggg tttccatacc
    23641 acctggggcg cgtttagctt cccgtttatt ttccttgcta ccgacctgac cgtgcgtatt
    23701 tttggcgcac cgctggcccg tcgtatcatc tttgcggtga tgatcccggc gttgctgatc
    23761 tcctacgtca tctcgtcgct attctatatg ggttcctggc agggattcgg cgcactcgcc
    23821 cacttcaacc tgtttgtcgc ccgtatcgcc accgccagtt tcatggccta cgcgctggga
    23881 cagatcctcg acgtgcacgt ttttaaccgc ctgcgtcaga gtcgccgctg gtggctggca
    23941 ccgacagcgt ccacactgtt cggtaacgtc agcgacacgc tggccttttt cttcattgcc
    24001 ttctggcgta gcccggatgc ctttatggct gaacactgga tggaaatcgc gctggtcgat
    24061 tactgtttca aagtgttaat cagtatcgtt ttcttcctgc caatgtatgg cgtattactc
    24121 aatattctgt tgaaaagact ggcagataaa tccgaaatca acgctttgca ggcgagttaa
    24181 aggttcgtta tcagagttgt gataagatgg atgaatgagc cgttatggcc gtttatcgaa
    24241 aggaagaagt caatgcgcaa tctggttaaa tatgtcggaa ttggcctgct ggttatgggg
    24301 cttgcggcct gtgatgataa agacactaac gctacggcgc agggttcggt cgcggaaagt
    24361 aacgctaccg ggaatcccgt caacctgctt gatggcaagt taagtttctc gctgccagcg
    24421 gatatgaccg accagagcgg taagctggga acgcaggcca ataacatgca tgtctggtcc
    24481 gacgccaccg ggcagaaagc agtcatcgtc atcatgggcg atgatccgaa agaagatctg
    24541 gcggtgttgg cgaagcgtct ggaagatcag caacgtagcc gcgatccgca gctgcaagtg
    24601 gtaaccaaca aagccattga gctgaaaggt cacaaaatgc agcagttaga cagtattatc
    24661 tccgcgaaag gccagacggc gtactcttcc gttattctgg gtaacgtggg taatcaactg
    24721 ctgaccatgc aaattactct gccagctgac gatcagcaaa aagcgcagac caccgcagaa
    24781 aacatcatta atacgctggt tattcagtaa gttttaagat gatgaggcgg cctcagggac
    24841 gtgttccgga ggccgttttt ttaatcgcca cgtcagtaat aacgcgattg cgaccagtcc
    24901 cgccgccgcc agataaatca ccggtacgcc cgcccagctc atcaccagcc cagccagtgg
    24961 tccggtcacg ccaagcgata aatccataaa tacggtgtaa gttgccagcg ccgccccctg
    25021 attttgctgc ggaaccgctt ttaccgccac tacgcccaat gccgggaaca ccagcgaaaa
    25081 ccctgccccc gccagtaaga cgccgacttt cgccatccac ggcatagtcg ccacgccaac
    25141 cagtagcagg ccgattatct caacgctaaa gcaaatcatc gccacgttta agccgccgat
    25201 acggttaatg ccgttaggga ataacaaacg cgtaccgaca aacgcacagc taaacagcgt
    25261 cagcgcgaaa gccgcaccgt cccaaccttt agcgtcataa aacagcgtga taaaggtggc
    25321 gatgacgcca aatccggcag aagccagtgc cagtgccata ccgtacagcc agacgcgccc
    25381 aagcaccgcg cgaaacggca gcggtttgcc tttactggct tttaccgtcg gacgcgggat
    25441 cgccaacaaa atggccacca gcgccacgcc cataatgatt aacgctaacg cctgcaagcc
    25501 gccccagtga taaaacacga cgcctaacgg cgcacccatc gccatcgccc cgtaattgac
    25561 aatgccgttc cacgaaatca ctcgcccgat atgcagcgag ccaaccacgc caacgcccca
    25621 cagggtcgat cccgttccgg caaaactttg cccaatccca aggatcacgc gtcccaggca
    25681 aagtaataac aggctgatga cgggcagact ggcggttaat cctgccgtca gatatcccag
    25741 accgctcaaa aagcagccgc ataaaccgaa gacgacaatc tttttggggc ccagcaaatc
    25801 ggcgtaacgt ccggcatgag gacggctcag caaggtggcg aaatattgca ggctgataac
    25861 caatcctgcc cagaaagcgc taaagcccat cacatcatgg acatagcccg gtaatacagc
    25921 gagcggcaac ccgatggtga ggtagctggc gaagttaaac atgacaatgg agacaatgcg
    25981 caaattcagg cgcaatccgt ttagcgcggg ttcggctacg ggttcgggca tgaggatcac
    26041 cacattttta caacagtgtt tcatttttac cacgtgctga cgtgaaaatc agcagtaaga
    26101 atcagaatat tgctggcgtg actcccgcta cacttaatac aaaaagtcac aaggaagccc
    26161 caatggaaat ccctcaaccc gataaaacgg gcatgcacat tctgctcaag ctggcctcgc
    26221 tggtagtgat cctcgcgggc attcacgcag cggcagatat cattgtgcag ctgttactgg
    26281 cgctgttttt tgccatcgtc ctcaacccgc tcgttacctg gtttattcgt cggggagtac
    26341 aacgccccgt tgccattacg attgtagtgg tggtgatgct gatcgcacta accgcgctgg
    26401 tcggcgtact ggcggcatcg tttaacgaat ttatctctat gctgccgaag tttaataagg
    26461 agctgacgcg caaacttttt aaattgcagg agatgttgcc ttttcttaat ttgcatatgt
    26521 cgccggagcg aatgctgcag cggatggact cggaaaaagt ggttaccttc accacagcgc
    26581 taatgaccgg gctttccggg gcaatggcga gcgtgctttt gctggtgatg accgtagttt
    26641 ttatgctgtt tgaagtgcgc cacgtccctt acaaaatgcg ttttgcgctg aataacccac
    26701 agattcacat cgcaggatta caccgcgcac ttaaaggcgt ttcgcactat cttgcattga
    26761 agacgctact cagtttatgg acaggtgtca tcgtctggct ggggctggag ctgatggggg
    26821 tgcagtttgc gctgatgtgg gcagtactgg cgtttttgct caactacgtg cccaatatcg
    26881 gcgcggtaat ttccgccgta ccgccaatga ttcaggtgct gctgtttaat ggtgtttacg
    26941 aatgtattct ggtcggcgca ttgtttttag tggtccatat ggtcatcggc aatattttag
    27001 aaccacggat gatgggccat cgcctgggga tgtccaccat ggtggtattt ctttcattgt
    27061 taatttgggg atggctgctc ggcccggtag ggatgctact ttcggtgcca ttaaccagtg
    27121 tgtgtaaaat ctggatggaa accaccaaag gcggtagcaa actggcgatt ttactggggc
    27181 caggcagacc gaaaagtcgg ttaccgggat gaggcgacaa gtgatacgat acgcactttc
    27241 attttccatt aaacgttggc cctgatatgt atcggatagt tctggggaaa gtttcgacct
    27301 taagcgcagc tccactgcca ccgggtttac gcgagcaagc accgcaaggt ccacgacgcg
    27361 aacgctggct ggcggggcgt gcattgcttt cgcacacgct ttccccgcta ccggagatca
    27421 tctatggcga acaaggcaaa cctgcattcg cgccggaaac gccgctatgg ttcaacttaa
    27481 gccatagcgg tgacgatatc gctctgctgt tgagcgatga aggcgaagtc ggctgcgata
    27541 tcgaagtgat tcgcccgcgc gccaactggc gctggctggc gaacgccgtg ttcagcctcg
    27601 gggaacacgc tgaaatggac gccgtgcatc ctgatcagca actggaaatg ttctggcgca
    27661 tctggacgcg caaagaagcc atcgttaaac agcgtggcgg cagcgcctgg caaatcgtca
    27721 gcgtagatag cacctatcac tcctcgctgt cagtcagcca ttgtcagcta gaaaatttaa
    27781 gccttgcgat ctgcaccccc acccccttta cgctcaccgc cgacagtgtg caatggatcg
    27841 attcagttaa ctgatccgcc cacccgactg cccatctatt gatccagaac aggtaatcag
    27901 tatgacgaat acttaaaatc gtcatactta tttccgccat ctattttaat ccattggggt
    27961 taccatgctc tccacactcc gccgcactct atttgcgctg ctggcttgtg cgtcttttat
    28021 cgtccatgcc gctgcaccag atgaaatcac taccgcctgg ccggtgaatg tcgggccact
    28081 aaacccgcac ctttacacgc ctaaccagat gttcgcccag agcatggttt atgaaccatt
    28141 ggtgaaatat caggcagacg gttcggtgat cccgtggctg gcaaaaagct ggactcattc
    28201 agaagatggt aaaacctgga ccttcaccct gcgtgatgac gtgaaattct ccaacggtga
    28261 accgttcgat gccgaggcgg cggcagaaaa cttccgcgca gtgctcgata accgtcaacg
    28321 tcacgcctgg ctggagctgg caaaccagat tgttgatgtt aaagcactca gtaaaacaga
    28381 gctgcaaatt accctgaaaa gcgcctacta tcctttcctg caagaactgg ccctgccccg
    28441 tcctttccgt tttatcgctc cctcgcagtt taaaaaccat gaaaccatga acggaattaa
    28501 agcgccgatt ggcaccggac cgtggatttt gcaggaatcg aaactgaatc agtacgatgt
    28561 cttcgtccgt aacgaaaact actggggcga aaagccagcg attaaaaaga tcacctttaa
    28621 cgtcatcccg gacccgacta cccgcgcggt ggcgtttgaa actggcgata tcgacctgct
    28681 gtacggaaac gaagggttat taccgctcga taccttcgcc cgctttagcc agaatccggc
    28741 ttaccacacc caactgtcac agccgatcga aaccgtgatg ctggcgctca ataccgccaa
    28801 agcccccacc aacgagctgg cagtacgtga agctcttaat tacgcggtaa acaaaaaatc
    28861 gctgattgat aacgcgttat atggcaccca gcaggtcgcc gacaccctgt ttgccccttc
    28921 tgtgccttac gctaatctcg gcctgaaacc gcgccagtac gatccgcaga aagcgaaaga
    28981 gttgctggaa aaagccggtt ggacgctgcc tgcgggcaaa gacatccgcg agaaaaatgg
    29041 tcagccgctg cgcattgaac tgtcgttcat cggcaccgat gcgttaagca aatcgatggc
    29101 ggaaatcatt caggctgata tgcgccagat tggcgcagat gtctcgctga ttggcgaaga
    29161 agagagcagt atctatgctc gtcagcgcga cggtcgtttt ggcatgattt tccaccgcac
    29221 ctggggcgcg ccatacgatc cacacgcctt cctgagttca atgcgcgtac cgtcgcacgc
    29281 tgacttccag gcacagcaag gattagccga caagccgctg attgataaag agatcggcga
    29341 agtgctggcg acccatgacg aaacgcaacg tcaggcgctg tatcgcgaca ttttgacccg
    29401 tctgcatgac gaggcggttt atctgcctat cagctacatc tcgatgatgg tggtatcaaa
    29461 accggagctg ggtaatatcc cctacgcgcc gatcgccacc gaaattccgt tcgaacagat
    29521 taaaccggtg aaaccttaat gttgcgttac gtattacgcc gctttctgct gctgatcccg
    29581 atggtgctcg ccgcctcggt gatcattttt ctgatgctgc gcctcggtac cggcgacccg
    29641 gcgctcgatt atctgcgttt atctaacctg ccgccgacgc cggagatgct ggcctctacc
    29701 cgcaccatgc tcgggctgga tcagccgctg tacgtccagt acggtacctg gttgtggaag
    29761 gcgctgcatc ttgactttgg tatctcattc gccagccaac gcccggtgct ggacgatatg
    29821 ctgaacttcc tgcccgccac gctggaactt gcaggtgcgg cgctggtatt aattctgctc
    29881 acttccgtac cgctcggtat ctgggcggcg cgccatcgcg accgtctgcc ggatttcgcc
    29941 gtacgtttca tcgcgtttct tggcgtgtcg atgcctaact tctggctggc gtttttactg
    30001 gtgatggcgt tttcggtgta tctgcaatgg ctacccgcga tgggttacgg cggctggcag
    30061 cacatcattt tgcctgcggt ttccattgcc tttatgtcgc tggcgattaa cgcgcgactg
    30121 ctgcgcgcca gtatgctgga cgtcgccggt cagcgtcacg tcacttgggc gcgtctgcgc
    30181 ggcctgaacg acaaacagac cgaacgccgc catattctgc gcaatgcctc gctgccgatg
    30241 atcaccgccg tggggatgca catcggcgaa ctgattggcg ggacgatgat tatcgaaaac
    30301 atctttgcct ggccgggcgt cgggcgctat gcggtgtcgg cgatttttaa ccgtgactat
    30361 ccggtgattc agtgctttac gctgatgatg gtggtggttt ttgtggtctg taatttgatt
    30421 gtcgatttgc tcaacgccgc gctggacccg cgcattcgtc gtcatgaagg agcgcacgcg
    30481 tgaacttttt cctctcttcc cgctggtcgg tacgcctggc gctgatcatt atcgccctgc
    30541 tggcgctgat tgcgctcacc agccagtggt ggctgccgta tgacccccag gcgattgatt
    30601 tgccgtcgcg cctgctttcg ccggatgcgc agcactggct gggcaccgat cacttaggtc
    30661 gcgatatttt ctcgcggctg atggcagcga cccgcgtgtc gctcggttcg gtaatggcct
    30721 gcctgctgct ggtgctgaca ttagggctgg ttattggcgg cagcgccggg ttgattggcg
    30781 ggcgcgttga tcaggccacc atgcgcgtcg ccgatatgtt tatgaccttc ccgacctcga
    30841 ttctgtcgtt ctttatggtt ggcgtgctcg gcaccgggct gaccaacgta attatcgcca
    30901 tcgccctgtc gcactgggcg tggtatgcac gcatggtgcg cagcttggtg atttcactac
    30961 gccaacgcga gtttgtgctg gcgtcacggc tttccggtgc gggccatgtg cgggtgtttg
    31021 tcgatcatct ggcaggcgcg gtgatccctt cgctgctggt gctggcaacg ctggatatcg
    31081 gccatatgat gctgcacgtc gcggggatgt ctttccttgg cctcggtgtg accgcgccga
    31141 ccgccgaatg gggcgtgatg attaacgacg cgcgccagta tatctggacc cagccgctgc
    31201 aaatgttctg gccggggctg gcgctgttta tcagcgtgat ggcctttaac ctggtgggtg
    31261 acgcactgcg cgatcatctg gatcctcatc tggtgacgga gcacgcacac taatgccgca
    31321 acagattgaa ctacgtaata tcgcgctaca ggccgcgcag ccgctggtac acggtgtatc
    31381 gttaaccctg caacgcgggc gcgtgctggc gttagtcggc ggtagcggca gcgggaaatc
    31441 attaacctgc gccgcgacgc tgggcatttt gcccgctggc gttcgccaga cggcggggga
    31501 aattttagcc gatggcaaag cggtttatcc ctgcgccctg cgcggcatca aaattgccac
    31561 catcatgcag aacccgcgca gcgcctttaa tccgctacac accatgcaca cccacgcgcg
    31621 ggaaacctgc ctggcgttag ggaaacccgc cgatgacgct acgcttaccg ctgccataga
    31681 agcggtgggg ctggaaaacg ccgcgcgcgt gctgaagctg tacccgttcg agatgagcgg
    31741 cggcatgttg cagcgcatga tgattgcgat ggcggtgctg tgtgaatcac cgtttatcat
    31801 cgccgatgaa ccgaccaccg acctcgacgt ggtagcacag gcgcgcatcc tcgatctgct
    31861 ggaaagcatt atgcaaaaac aagcgccggg aatgctgctg gtcacccatg atatgggcgt
    31921 tgtggcgcgt ctggcggatg acgtggcggt gatgtcacaa ggtaaaattg tcgaacaggg
    31981 cgatgtagaa acgctgttta acgcccccaa acatgcggta acgcgcagcc tggtttccgc
    32041 tcatctcgcc ctctacggta tggagctggc atcatgactt tacttaacgt ctccggcctt
    32101 tcccatcact atgcccacgg tggatttaac ggaaaacatc aacatcaggc ggtgctgaat
    32161 aacgtttccc tgaccctgaa aagcggcgaa accgtcgccc tgctggggcg cagcggctgc
    32221 gggaaaagta ccctcgcgcg gttgctggtg ggtttagaat cgcccgcgca ggggaatatt
    32281 agctggcgtg gcgaaccgct ggcgaaactc aatcgcgccc agcgtaaagc gttccgccgc
    32341 gatattcaga tggtatttca ggactccatc agcgccgtga atccgcgcaa aaccgtgcgc
    32401 gagatcctgc gtgaaccgat gcgccacctg ctatcactga aaaaatccga acaactggcg
    32461 cgcgccagcg aaatgctgaa ggcggtcgat ctcgatgaca gcgttctcga caaacgccca
    32521 ccgcagttaa gcggcggcca gctccagcgc gtctgcctgg ctcgcgcgct ggcggtcgaa
    32581 ccgaaactac tgattctgga tgaagccgtt tctaaccttg atctcgtgtt acaggcgggc
    32641 gtcattcgcc tgctgaaaaa gctacaacaa cagtttggca ccgcctgcct gttcatcacc
    32701 cacgacttac gcctggtgga acgcttctgt cagcgggtga tggtgatgga caacggacaa
    32761 atcgtcgaaa cccaggtggt gggagataaa ttaacctttt cctctgacgc cggacgtgtg
    32821 ctacaaaacg cggtattacc cgcattcccc gtgcgccgtc gcaccacaga aaaggtttaa
    32881 cgcaaatgca acgagtcacc atcacgcttg atgacgattt actggagacg ctggacagcc
    32941 tgagccagcg tcgtggttat aacaaccgtt ccgaagctat ccgcgatatt ctgcgtagcg
    33001 ccctggccca ggaggccact cagcagcacg gcacgcaagg tttcgcggtg ctctcctatg
    33061 tgtatgaaca cgaaaaacgc gacttagcca gccgcattgt ctccacccag caccatcacc
    33121 acgatctctc cgtcgccacg ctgcatgtgc atatcaatca cgacgactgt ctggaaatcg
    33181 ccgtgctgaa aggtgatatg ggcgacgtgc agcattttgc cgatgacgtt atcgcccagc
    33241 gcggcgtgcg gcatgggcat ttgcagtgct tgccgaagga agattgagtt atttctgaaa
    33301 cgaggaaaga aaagatatta actgaactta atttataaaa ttaagttcag ttgcatagat
    33361 agatcctgta aaggcagata ttctgcgtaa tgtacgccgc taaaatttat aaatgattca
    33421 aacgctcatt cagtgtttca ataatatagg tattaacaga gacttgttgc tgcgcagccg
    33481 cattattaag gcgttcactc agcgattctg gatagcgtaa cgtaaaggtc ttaatctttt
    33541 ctgtacgcgc atacggctca atacccgcag ctttacaatc ctcaagatac tctcgcaggg
    33601 aaagttcccc ttctttttgc agcccctgaa tgctgtcaga gacaaaatca caataacctg
    33661 acaagcccag gaatttgcca cgaaatgcat tcagttctgg cacgtaggta ataacagctg
    33721 gttgcccggc aatctccata gagtttggcg tcttcagttt gatcatggtt ttacccctat
    33781 acttagtaac cagtcacgca cgctttctac agcgccttta tcggtatctg gcgacggatg
    33841 aggccgatgg aaacacgcaa cgctcatatt tagtatgaac ttacaacgcg aacctcttcc
    33901 ttccttaatt tctccgccta atgctttaac cagtgactct atatcagacc acttgatccc
    33961 ttgaggaaca ggagttttaa atatttgatc caatgtattt ttttttcttc cgtaaagcct
    34021 gtaccttggt tctcatggca tgtccttatg acttcacttt atgacatcaa tagtatgaca
    34081 tcacagtatg aagtcaagtt tcccttcctt acgccattgt cccaatcgtc ttcctgaatc
    34141 gcagcagcgc aatggtgaaa aacgcgccgc caattgccat cagcgtcagg aactgcggcc
    34201 agacgatttc gaatccggca ccccggtaga gaatggcctg cgcgaggcta acaaagtgtg
    34261 tcgtcggcat ggtcagcata atgtcctgca ccatctgcgg catactttcg cgcggcgtgg
    34321 atccaccgga aagcatttgc agcggcagca gcaccagaat caccagcagc cccagttgcg
    34381 gcattgaacg cgctatcgtc cccataaaaa tgccgattga cgtggtggca aacagactga
    34441 gcgccacgcc cagcataaac agcgggatcg agccttcaat cggtacgccc agtacacctt
    34501 tcgccatcag caccagcgat aatcccgata ccaccagcac caccagcccc atcgaccaga
    34561 tcttcgccat catgatctca aacggcgtta tcggcatcac cagtaagtgt tccaccgtgc
    34621 cgtgttcacg ctcgcggatc agcgccgatc cggtcaatac aatcgccagc atggtaatgt
    34681 tgttgatgat cgccatcacc ccgccaaacc acgcgggatc gaggttcggg ttaaagcgca
    34741 tccgggtttc cagcgatacc aacggttcgc tgttatcacg gtagcgcgcg acaaagctgt
    34801 tcacttcacc gttgataata ttctggatat acccattgcc ggtaaatgcc tggctcatgc
    34861 gcgtggcatc gacgttcacc tgaatatccg gctggcgtcc ggcgaggaca tcacgctgaa
    34921 aattaggcgg aatatttatc gcgaaggtat agcgtccggc gtccagtccg gcatccatct
    34981 catcggcggt gatcatctcc ggtggcaaaa accacggacg atagaagctg ttaacgatcc
    35041 ggttcgataa ctgcgattga tccatatcgg caatggcgat cggcgcgagg ttcaacgatc
    35101 ctggcgtaac ggtcgctgac gaatacaccg acaccgtaaa cgagaagaca atcagcgtca
    35161 gcatcgcttt atcaccgagc agactgcgca actctttgat acccagatta aaaatattgc
    35221 gtaaatggcg catcatccct cctgtttttt cagcagcagg atgcttaagc ccatcaccag
    35281 cgggatggct atcagcaacg ggataaaaag ttgccacaaa tcagtcagat ccagcgcttt
    35341 cgagaacgtt ccgcgggcga tagtcagaaa atgactggtc gggtaaacct cgccgatcca
    35401 acgtccaggc ccttccagcg aagctaccgg atcgatcatc ccggaaaact gtgtcgccgg
    35461 gatcaacgtg ataatcgccg ttccgaaaat ggcggcaatc tggcttttca taaaggtgga
    35521 gatcagcagc cccattccgg tggcaatgat gatatacagc agcgccgcca gggtgagcgt
    35581 caggaaactg cctttatgcg gtacgccaaa cacaaacacc gacaggccgc agagcaggaa
    35641 aaagttcagc atccccagcg cgatgtacgg cagctgtttg ccaagcaaaa attcgctacg
    35701 ggtggtgggg gtcacgtaaa ggttgataat cgaccctagc tctttttccc gcaccacgct
    35761 aagggcgctt agcattgacg ggatcatcat cagcagaagc gggatcaccg ccggaacaat
    35821 cgctggcagg ctttttacgt ccgggttata gcgatagcgc gtctcaatgt tcatcagccc
    35881 gctttggctg gcgggtgtcg attgtcggct cgccacatcc tgaagccaac tctggtgcat
    35941 tgcctgcacg taacctttta ccgtttcggc acgactgggc atcgctccgt cgatccagac
    36001 gccgagttcc acaggcgtac cacgcgcgat atcgcgcccg aaattgggcg ggatctcaat
    36061 cgccaccgtg atatcgcccg cacgcatccg acgatcaagc tcgtcataac tggtgagcgg
    36121 cggctgttcg ataaagtaac gggaaccgga gaggttgagt gtccacgcct ggctactgac
    36181 ggtctggtcg cggtcgagca ccgcaaagcg caggttttcc acatccatac tgatgccgta
    36241 acccattatc agcatcagga tcaccgttcc catcagcgcc agcgtcgaac gtactggatc
    36301 gcgtcgcagt tccagcgctt cgcggcggct gtagctaaac agacggcgca ggctaaatcc
    36361 ctgacgcggc gcgtgggtgg tgtcgtgtac cacgggcggc gcttcggctt cgttgctctg
    36421 ccctgccgct tcctgcaaat aggcgataaa tgcctcttcc agactggcgg ctccgcgttt
    36481 ctcaaccagt tcctgcggtg taccgctggc aagcactttt ccggcgtgca tcagtgagat
    36541 gcggtcgcaa cgttccgctt cgttcataaa gtgggtagag atgaagatag tcactttgtc
    36601 ctggcgcgag agatcgacca tcaactgcca gaacatatcc ctcgccaccg gatcgacacc
    36661 agaagtaggc tcatcgagga ttaacatctc cgggcgatga atcaccgcca ccgccagcga
    36721 aagccgctgg cgaatgccga gcggcaatga ctccggcaga acatcttcaa cgtcgttgag
    36781 cttaaaacgc tcgctcattt cagccactct tgcgggaatt tccgcttccg ggatgtgaaa
    36841 caaacgggca tgtaactcaa ggttttgccg cacggtgagt tcgttataga gcgaaaacgc
    36901 ctgcgacata tagcccaccc gacggcgggt atcgatatct tttggatcaa ccggttgccc
    36961 gaacagccac gcctcacctt cgctggcggg cagcagtccg gtgagcattt tcatggtggt
    37021 ggatttaccg cagccgttcg aaccaagaaa accaaaaatc tccccgcgtg gaatgcggaa
    37081 attaacgtga tcaacggcaa cgaaggaacc aaaacgcatg gtcagatcgc gcgcttcgat
    37141 ggcaatctct gcgttttcag gttgatacgg tgggatcact accgcctgat gcgcctggcg
    37201 ttgcgcttgc ggtaacagat ttataaatgc ttcttccagc gtagcgcttt gcgtttgctg
    37261 ccgtagctct tcggcgctgc cagttgccag cacttctccg gcattcatcg ctaccagcca
    37321 gtcgaagcgt tcggcctctt ccatataggc ggtggcgacc agcacgctca tattgctctg
    37381 ccgctggcga atactgtcga tcagatccca gaactgggag cgggagagcg ggtcaacccc
    37441 cgttgttggc tcatcaagga tcaacagttc cgggtcgtgg attaacgcgc agcacagccc
    37501 aagtttttgc ttcatcccgc cggagagttt ccctgccgga cgatcgcgaa acggtgctaa
    37561 ccccgtgctg gtcagcagct cattaattcg cacttcccgc tccgctttgt cgtgaccgaa
    37621 caggcgagcg aaaaaatcga cgttttcata caccgacaag gtgtggtaga ggtttttgcc
    37681 cagcccctgc ggcatccagg cgatgcgcgg gcagacgtcg cggcgatgcc tcgggtcgcg
    37741 catatcgccg cccagcacca tcacattgcc ctgctcaatg acgcgggcac cggaaatcaa
    37801 cgacaataag ctcgacttcc cgacgccatc cggaccaatc agcccgacca tacagcgggc
    37861 cggaatatcg agagtaatat tgttcagcgc aacgtttttt ccataatgct ggctcacgcc
    37921 cgccagttgc gcgacaggcg ggacgggaac cagttccaga tgcgtcattg cggcaacctc
    37981 accacgaggt cgtcaggcca cggaagttct tcattcaccc gcacccacgc tacgcccggt
    38041 aaaccggttt tgacatattc cagatgctgc tggagtaatt ccggtgggat acgcgctttg
    38101 acgcggaaca tcagtttcag ccgttcatcg ctggtttcga cggtttttgg cgtgaactgg
    38161 gcgacactgg cgacaaaact gatggtcgcc ggaatacgca gatctggcgc ggcatcgagg
    38221 atcagccggg cttcaccgcc aagtttcagc gtgcccgcct gttcggttgg caggaagaaa
    38281 gtcatataga cgtcgctgag atcgaccata ttcagcaccc gaccgcctgc cgccagcact
    38341 tcccctggct cagcaacccg atactgcacg cgtccgtcac gcggggcttt cagttcactg
    38401 tcatcgatat ctgcggcaat acgccgttca gtggcttgcg cggcttcgac gcgagtttgg
    38461 gcctgaatga tattggtacg ggccgcttct atagccgctt tagaagccga tacctgagct
    38521 tttgccgatt ccagcgcagc tcgggcgctc tcagcggcgg cgcgatcgtc atccagctgt
    38581 tgcgcagaaa tagcccctcg ttgggcgagc gagcgggaac gcgtatgacg tttagccacg
    38641 gagtccagtt cggactggcg ttgattaacc agcgactgcg cggcacgagt ttcgctttgc
    38701 cgttgctcca gcaaagcctg cgcggcagca acggcacttt gtgcttcttt gatttgcgcg
    38761 atggcttcca gtcgctgctc ctgcaacacg cgagtatcca tcttcgccag cacttcgcct
    38821 tcgcgaacaa actggccttc tttcaccaga atggtgtcga tacgcccggc aattttgctg
    38881 gcgatatcca cttccgtcgc ttcaatgcgc ccattgctga cagcaaagcc ttccggcaca
    38941 cccgccgggc gcaacagcca ccacgccacg acggcagcca ccaccagtaa cccgacaacc
    39001 caccacgcca gatggcgctt actcttatcc ataatcgacc cgccataatc cctgcaagca
    39061 aacgacgcgt gttacaactg ccgccgaacg acgttcagcg gattcttcca tccgggataa
    39121 gaaggaaacg acaaatacga ccagcgcgca gagcattgag cgccaacggg gaaggcttag
    39181 tgaaaacggg tagtaatgtt gtcatcatca gcattcctgg ccgtaaatga aacacgcata
    39241 ctcataagtg tgatctataa aactggctga caaaacggta tccatcctgt tggatagcgt
    39301 ttcacgcctg ttcagttgca ggaacagagc attccttttt acgttttatc aacagtctca
    39361 accagatagt aacattaata ttcatgttgt ctatggttca gagctgtaac attgtgcgcc
    39421 ttctgatcaa aggcataagt ccatttctgt ttttttcatg ctggtaagac ttccagggag
    39481 tggttatctc aggccctgca ccagggttaa tttggcaatt atgcatgctg aacattcttc
    39541 cagcatgttt actgtcacgg gcgttaagac ctaaaactgt ccccgtttca cctatacatt
    39601 ctcttccacc attaccgtta tgtgcaacaa cttgatttat acctaacatt tgtgagcatg
    39661 ctaatagtgc ttcaagttta ggtctgtaat ctgtcgaaac agaaaaataa cgattaaagg
    39721 atcgacaaga gatagcccgg ctatttttaa actttttgcc attaattaca gcatggtgct
    39781 ttttattgat aattgccgct aattcgaccg gatcgagagc attggtcatt tcatcactag
    39841 agactgttat cggtcctata atgtaacgtt ttcgctgatc atcaaaagtt atcccatgat
    39901 gatttgccat tattttgtat ttggggtcat agaaacatac aggataatct tttatcccag
    39961 agtaggtatc ctcatcaagt ggtttcatgc gcgtgaagtt ctgcatatag ttaccattgc
    40021 aattcgtttc atgatttcca gctaatacat gaacgttttc attgatactc attatgcgtt
    40081 ttaataaatc gatgataaat ttatcgccac agatattact aaatcgatcg ccagtctggt
    40141 cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aaccaaataa ggctgaagac
    40201 tttctaaaac aatgtctctt ctgttcagga tactgattat tatattaaca ttagtgtcaa
    40261 gttgctcatt aaagttctcc cttagcaata cgttgagaac ttcatcttct atattaagca
    40321 actggctaag gaaattttct ccttcagcca tgattcccaa atgtcccgtt gcaaaaagcg
    40381 cgtacaaaac tgcaccgact gagccatcgg tatcgccaaa ataggtcggt ctggtacttg
    40441 tgggatcaac aggtgaacaa ttggagaaat aggttctgtc agcgaactgg ggttttatca
    40501 ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc cagatgggtt
    40561 tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata ttattaagta
    40621 aataaacaat gctgcaccaa ttaatatcgg tattatcaag agggcaaccg taatttttac
    40681 tattaattgt cacaataaac tgataacagt tattcctacg tagaatatga gcgcgcatgc
    40741 ccgatagcat actatatttt tctgaatgat tcgtttgtat ggtaaatgaa attttgtcat
    40801 cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc acaacctcac
    40861 cgagcattgt cctggcaata cttgatatat catcacctat accatttgca atatacccaa
    40921 acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt gtgattgtcg
    40981 ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc atattattta
    41041 ccgcgaggaa tacatagtta acatttaatc ttatgacata cacctcatgt tcgatgggaa
    41101 aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat ttcttatctc
    41161 atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgcct ctacaatcga
    41221 ttaagaaaac ctatgaaata ctggcaaaat taaaaagaga taccacagca ctcatgaagt
    41281 tttattgagg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca cgttcgttag
    41341 gtgctatctt ccaggaattt agccccatgg tataattatc aattttttga tgctgatcta
    41401 cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt ccacatttga
    41461 agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattt tcatatacag
    41521 gtgtggatgc tacagcattg cttctaatta gatggataag acgatcatcc atgtgttgtt
    41581 tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct ttcatctgat
    41641 taaatggaac ctgcaaagca ccgagacaat aacattcatt ttcttcattt cttattattc
    41701 cgtgatgact ggttaaaact ttcgtttctg agtcataatg acaaacatcg agcgttttaa
    41761 ttaagttata cgtatcaccg gcagagagtt tataattttc tagtcgtgca gtatagttac
    41821 cattaaaatt gatttcatga tttccagcca gaacgatgac atttttatta atacggctat
    41881 ctttattacc atccatattt cgcatagaat ttagcaaagt aaggatatat ttatcaccaa
    41941 aaatagtact aaatctatcg cctgtatgat ccccaaggaa gatacaaggg gttatcgcat
    42001 cgtcctttaa aaaaacatgt tgatgcaact cattcaatat catgtctctc gtatttaata
    42061 aattgtagag caatttatta tttttatgta tgtatgcaga tgaagccatg ttttcgtgtt
    42121 ttaataattc acataataaa ctccaaccct tttccctgat gcctatatgc cctgaaacga
    42181 gcaatgcata aagtacagcg cctactcttc catctgtatc gccaaaataa gccgtgttgg
    42241 cattttgtgg gttaatcaca ccacattgtt tataaaagtc tattttagaa aacacagatt
    42301 taacatagct atcaaactta tccttaggaa acgtctcttt agagacatcc caacgccagt
    42361 cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata gaaagcccat
    42421 taactttata agttatgtct gatttataaa ttatgtctga tttatctccg gagaaattaa
    42481 taaaataata accatcctta cactcaatct taactgtaat gggagacgat aattttatca
    42541 catggttatt gtctggtata attgtaaaga taaacgtgtt agtagtatta tcggcgaaac
    42601 tattatattc ttgaatatct atagatggct cactattgta aaaccatatt ttctccatct
    42661 gaagactatg ttcatttacc ctccagtgac catgttgcag gaaatagatg atattcaggc
    42721 ctaaatcgtt atctatttgc gaaaagtcat tcagcatatt aacttcaact gtgtttgatc
    42781 gatatttttt atcaaccttg ccatggtgtt gatgactgtt aactttatct gaatgcttat
    42841 cttgatggtt tgaagagaag tgcttaaata tattgtgaaa tactttcata aataacctgt
    42901 aatattttta cttgttagta acaaaataaa caatacatgt ctgaaagaca tatttacgat
    42961 gatacaaagc aataatactt atatgttcga tgattttaag caccgagact atccggtctt
    43021 ttactaaata atggaaaaca atccatccct ggaagataat tgtaaaaaaa aacaacagaa
    43081 taagaattaa tcttaggata aatatttatt tatcatggcc ttttaaccgg taaaaaatgg
    43141 cactcggcac agtaaacccc gctatagtta tgctatgcat ttgtctttaa tgatatctcg
    43201 tcgttatatt aacaggctga aattatcatt aatgcacatt tcattattag cagggatacc
    43261 tgaggagttt aaagtgaaca tatatatcgg gtggcttttc aaattaatcc ctttgattat
    43321 gggattaatt tgcatcgcgt taggtggctt tgtgctggaa agttcagggc aaagcgagta
    43381 tttcgtcgcg ggtcatgtgc tgatttctct ggcggccata tgcctggcat tattcactac
    43441 cgcatttatt atcatttcgc agctcacgcg cggcgttaat acgttttaca atacattgtt
    43501 ccccattatt ggctatgcgg ggtcaattat caccatgata tggggttggg cactgttagc
    43561 aggcaatgat gtgatggcag acgagtttgt cgccggccat gttattttcg gcgttggtat
    43621 gattgccgcc tgtgtatcga cggtggcagc gtcatccggt cactttctgc tcattcccaa
    43681 aaatgcagcg gggagcaaga gcgatggaac gccggtacag gcttattctt cattaatcgg
    43741 taattgcctc attgccgttc ccgttttact caccctgctc ggtttcattt ggtctattac
    43801 gctgttacgt agtgctgaca taactccgca ttatgtcgcg ggtcacgtat tgcttgggtt
    43861 aaccgcaatc tgtgcctgtc taattggcct tgttgccaca attgtccatc aaacacgtaa
    43921 tacgttttca actaaagaac actggctgtg gtgttattgg gttatttttc tcggctcaat
    43981 cacggtactg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    44041 cggcattatt cttatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    44101 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgatacccgt
    44161 cttcacctgc ctgttttgcc ttttcctggc atcatttctt gcggaaatgg cgcagaccga
    44221 catgggatat tttattcctt cgcgagttct ggtcggtttg ggagcggtat gctttacgtt
    44281 gttctcaatc gtttcaatat tagaagcggg ttctgctaaa aaataattgc aacgtaccgg
    44341 ataaaaccag cgttgaccat ttgcgtaacg ctggtttttc ttaggcatca tgaaataacg
    44401 cagcattaat gcatagtggt taaggataaa aaaacaacaa agttactgtc tttttcagcc
    44461 tgttcatatt ttataaagat ctggccactg ggtgagtttt caatcacctt tccttccacc
    44521 ttatattaag cctggagggt ttcagttcac gggctcatta gaaaataatc acaagatagc
    44581 cactatcaaa attagctcat ttttaatgcg gctctaataa ttttcatctt taggtaacag
    44641 gtcaggacga cttcgctgca atcaaatcct gcgcacaagc ccacgcactc gaccacgccc
    44701 actggaagtt atagcccccc agccagccgg taacgtccat cacttcgccg atgaagtaca
    44761 gcccaggcac tttgcgcgct tccatcgtcc gtgaagagag ttcgttggtg tccacgccgc
    44821 cgagcgtcac ttcggcagtg cgatagcctt cagtgccgtt gggttgtacg cgccagtcgg
    44881 tcaatgtgct aatcagtgcc tgttggtcac gcacgttgag ctgttttagc gaaacattcg
    44941 ggatttgccc gagttgctgt aagcgttcaa ccaaccgctt cggtagatga accgccagtg
    45001 tgtttttcag gctctgattc ggatgtgcgt tacgctgctc attcaggaag gtttcgagat
    45061 ccacatccgg tagcagattg atactgacaa attcacccgg ttgccagtag ctggaaatct
    45121 gcaacaccgc cggtccagac aagccgcgat gggtgaagag taagttctca cggaaaacga
    45181 tgccgttttc agcggtaatc acggaaggca ccgccacgcc cgccagcacc tgtaactctt
    45241 cgagcaacgg tttatgcaga gtgaatggca ccagacccgc acgggtcggc agcacgttga
    45301 ggccaaattg ttcggcaatc ttataaccaa acggcgacgc gcccagcccc ggcattgaca
    45361 gcccaccggt cgccatgacc agcttttcgc aaccgacagt catgccgttc agatcaagcg
    45421 tgaagcctgt ttcatcctcc gccacactca gcacttcgct acgcaatctg aaggtcacat
    45481 tgcccttctc gcactcatcc accagcatgt cgacaatctg ctgcgcggag tcatcgcaga
    45541 agagttgccc taacgttttc tcgtgccatg cgatgccgtg tttattgacc agatcaatga
    45601 aatcccactg ggtaaaacgt gcgagtgcag acttacaaaa atgcggattc tggctcagat
    45661 aagcgcctgg ttcgacataa aggttggtaa agttgcagcg cccaccgcca gacataagga
    45721 ttttgcgccc tggtttttta ccattatcga tcagcagaac ccggcgtcct gcctgacctg
    45781 ccagcgcaga acagaacata cccgccgcac cagcgcctat aataatggca tcaaaccttt
    45841 ccacgttgcg ctcctcttag aaaaaacggg cgtgaattgt aaagattcct cagtggtcgc
    45901 accagcatca atattactaa aaggaagtat ttgcctgaat tatataagat aattattttt
    45961 tgagtgaaat ccatacagga ggcaaatcaa aaaaagtcta tatttcactt tgcccgcgcc
    46021 gcgaaagtca ctgataatgc gccgcgttca tgtcctcaaa atggcgtaac gtcctatgct
    46081 acatttgttt gctggcctgg atttgcatac cgggctgtta ttattgcttg cactggcttt
    46141 tgtgctgttc tacgaagcca tcaatggttt ccatgacaca gccaacgccg tggcaaccgt
    46201 tatctatacc cgcgcgatgc gttctcagct cgccgtggtt atggcggcgg tgttcaactt
    46261 tttgggtgtt ttgctgggtg gtctgagtgt tgcctatgcc attgtgcata tgctgccgac
    46321 ggatctgctg cttaatatgg gatcgtctca tggccttgcc atggtgttct ctatgttgct
    46381 ggcggcgatt atctggaacc tgggtacctg gtactttggt ttacctgcat ccagctctca
    46441 tacgctgatt ggcgcgatca tcgggattgg tttaaccaat gcgttgatga ccgggacgtc
    46501 agtggtggat gcactcaata tcccgaaagt attaagtatt ttcggttctc tgatcgtttc
    46561 ccctattgtc ggcctggtgt ttgctggcgg tctgattttc ttgctgcgtc gctactggag
    46621 cggcaccaag aaacgcgccc gtatccacct gaccccagcg gagcgtgaaa agaaagacgg
    46681 caagaaaaag ccgccgttct ggacacgtat cgcgctgatc ctttccgcta tcggcgtggc
    46741 gttttcgcac ggcgcgaacg atggtcagaa aggcattggt ctggttatgt tggtattgat
    46801 tggcgtcgcg ccagcaggct tcgtggtgaa catgaatgcc actggctacg aaatcacccg
    46861 tacccgtgat gccatcaaca acgtcgaagc ttactttgag cagcatcctg cgctgctcaa
    46921 acaggctacc ggtgctgatc agttagtacc ggctccggaa gctggcgcaa cgcaacctgc
    46981 ggagttccac tgccatccgt cgaataccat taacgcgctc aaccgcctga aaggtatgtt
    47041 gaccaccgat gtggaaagct acgacaagct gtcgcttgat caacgtagcc agatgcgccg
    47101 cattatgctg tgcgtttctg acactatcga caaagtggtg aagatgcctg gcgtgagtgc
    47161 tgacgatcag cgcctgttga agaaactgaa gtccgacatg cttagcacca tcgagtatgc
    47221 accggtgtgg atcatcatgg cggtcgcgct ggcgttaggt atcggtacga tgattggctg
    47281 gcgccgtgtg gcaacgacta tcggtgagaa aatcggtaag aaaggcatga cctacgctca
    47341 ggggatgtct gcccagatga cggcggcagt gtctatcggc ctggcgagtt ataccgggat
    47401 gccggtttcc actactcacg tactctcctc ttctgtcgcg gggacgatgg tggtagatgg
    47461 cggcggctta cagcgtaaaa ccgtaaccag tattctgatg gcctgggtgt ttacccttcc
    47521 ggctgcggta ctgctttccg gcgggctgta ctggctctcc ttgcagttcc tgtaatcgta
    47581 cgcaccaaaa cgagcgggtc agctggcccg cttcagattg tgacatagtg cgctttgttc
    47641 atgccggatg cggcgtgaac gccttatccg gcctacaaaa acatgcaaat tcaatagatt
    47701 gcagagatta tgtaggcctg ataagcgtag cgcatcaggc aattttgcgt ttgtaatcag
    47761 tctcaagcgg gtcagtttac tggcccgctt tttttatgcg ttattattgc tgaagtgctt
    47821 aatgccaaat catcaatgca atcaggctga ccaccaccag accacacaat gcgctggtca
    47881 gaataaactg ccgacgcacc cgctcacagc ggcgaataaa ttcatcatcg tgatgatcgc
    47941 gataacgttg ggcatagata taccaaacga gacgcacctg tttgttgggt tggccatgtg
    48001 aggtaaaaaa gccccctcca tcaacatatt gatagagcaa tggatcgcag ttacgcagta
    48061 ccactaacaa cgcgcgtagt gatgagaaat agcgcgccat gttaacaatg caaacgacac
    48121 ataaagccca aaataatgcg acggtgctta tcatacctcc tccccggcga cctgcccgcg
    48181 gagttccacc ccggggctac cgctcccgat acgctgccaa tcagttaaca ccaggtcctg
    48241 gagaaaccgc ttttgtggtg accaacatcc gagcggctct atagatagtg taggagatca
    48301 ggttgttttt tttccagaag gttaaccact atcaatatat tcatgtcgaa aatttgttta
    48361 tctaacgggt aagcaaggcg gattgacgga tcatccgggt cgctataagg taaggatggt
    48421 cttaacactg aatctttacg gctgggttag ccccgcgcac gtagttcgca ggacgcgggt
    48481 gacgtaacgg cacaagaaac gctagctggc cagtcatcga caactttatg gaaggagtaa
    48541 cactatggct tataaacaca ttctcatcgc ggtcgatctc tccccggaaa gcaaagttct
    48601 ggtagagaaa gcagtctcta tggctcgccc ctacaatgcg aaagtttctc tgatccacgt
    48661 agatgtaaac tactctgacc tatacaccgg gcttattgat gtgaatctgg gtgatatgca
    48721 gaaacgcatc tctgaagaga cacatcatgc gctgaccgag ctttccacta atgcaggcta
    48781 cccaatcact gaaaccctga gcggcagcgg cgacctgggc caggttctgg tcgatgcaat
    48841 caagaaatac gatatggatc tggtggtttg tggtcaccac caggacttct ggagcaaact
    48901 aatgtcttcc gcacgtcagc tgatcaacac cgttcacgtt gatatgctga ttgttccgct
    48961 gcgcgacgaa gaagaataat catcccccca cgacgtgttc ctgaacgccc gcatatgcgg
    49021 gcgttttgct ttttggcgcg ccttgttacc tgatgcagcg taaacccctt atctggccta
    49081 cgatctgcgt acgcaatcaa aatctcctgc caatacaaca tttaacacta tcatattttc
    49141 catcattagt gtgatcagct ggttattttc tgttgtaata gtgtattaat ctattcaccg
    49201 catcaatatt aagaatctct gacagatgta aactttttcg cgcgttatcc cttacgcgtt
    49261 catacttttc aggatggtat tggaaggtta ataaatatga atacaacaac acccatgggg
    49321 atgctgcagc aacctcgccc atttttcatg atcttttttg tcgagttatg ggagcgattc
    49381 ggctactacg gcgtgcaggg cgtactggcg gttttcttcg ttaaacagct tggattctcg
    49441 caagagcagg cttttgtcac ttttggtgct tttgctgcgc tggtctatgg cctcatttcc
    49501 attggcggct atgtcggcga ccacctgctg gggaccaaac gcaccattgt tctcggagca
    49561 cttgtgctgg cgattggcta cttcatgacc ggcatgtcgc tacttaagcc tgacctgatt
    49621 ttcatcgccc tggggactat cgctgtcggt aacggcctgt ttaaagctaa cccagccagc
    49681 ttgctttcga agtgctatcc gccgaaagat ccgcggcttg atggcgcatt caccctgttc
    49741 tatatgtcga tcaatatcgg ctcgttgata gcgttatcgc tggcccctgt gatcgctgat
    49801 agatacggtt attcagtcac ctacaacctg tgcggggcgg ggttaattat cgcattactg
    49861 gtttacatcg cctgtcgtgg aatggtgaaa gacattggtt ctgaacccga cttcaagccg
    49921 atgagcttca gcaaactgtt gtacgtatta cttggcagcg tggtgatgat cttcgtatgt
    49981 gcatggctga tgcacaacgt agaagtcgcc aatctggtgc tgattgttct ctccatcgtc
    50041 gtcaccatca tcttctttcg tcaggcattc aagctggata aaaccgggcg caataaaatg
    50101 tttgtcgcct ttgtcctgat gctcgaagcg gtggtgtttt acattctcta cgcccagatg
    50161 ccaacgtcgc tgaacttctt tgccatcaac aacgtgcatc atgaaattct cggcttttcc
    50221 ataaacccgg tcagcttcca ggcgcttaac ccgttctggg tggtactcgc cagcccaata
    50281 ctggcaggca tttacacgca tctgggtaac aaaggcaaag acctctcgat gccgatgaaa
    50341 tttactctcg gcatgtttat gtgctcactg ggctttttga cggcggcagc tgcgggaatg
    50401 tggtttgcgg atgcacaagg gctgacatcg ccatggttta tcgtgctggt gtatttattc
    50461 cagagcctgg gtgaattgtt tattagcgcc cttggcctgg cgatgattgc tgccctggtg
    50521 ccgcagcatt tgatgggctt tattctcggg atgtggttcc tgacgcaggc tgccgcattc
    50581 ttgctgggcg gctatgtggc aacatttacc gcagtgccgg acaacattac cgatccgctt
    50641 gagacgttac ccgtctatac caacgtgttt ggtaagattg gcctggttac gctgggcgtt
    50701 gcagtagtga tgctgttgat ggtgccgtgg ctgaaacgca tgattgcgac gccggaaagc
    50761 cattaattat tcttgcagaa agcagggtag cgttatcgct accctgcttt acttttacac
    50821 gggcgtacct gcatagatat caaagcgatg ccctttagtc acaaccgcgt ttggcgtggc
    50881 gacattcgct agcggtggcg cgtagtccgg gcgctttact accacgcgtt tggtcgccag
    50941 caagcgtgca ggctccagca atccatcggc atcaagatcc ggccccacca gcgactgaaa
    51001 gacacgcatc tcttttttca ccagcgcgct tttctgctta tgcgggaaca tcgggtcgag
    51061 atagaccacc tgcgggcgtg gggtaatatc ggttagcgcc gtcaggctgg aggcgtgaat
    51121 taactgcaaa cgctcctgca accagccgcc gatttccgca tccgcataac cacgcgccag
    51181 gccgtcgtcg agcagcgcgg caacaactgg attacgctcc agcatccgca cgcggcaacc
    51241 gactgaagcc agcacaaagg catcgcgccc cagtcctgca gtggcatcca ccacatccgg
    51301 caaataatcg cctttaatgc ccaccgcttt cgccaccgcc tcaccgcgac caccgccgaa
    51361 tttgcgtcgg tgcgccatcg ctccgccaac aaaatcaaca aagatgccgc caagttttgg
    51421 ctcatcacgc ttgcgcaatt ccagatgttc cggcgttaac accagcgcca tcaggttgtc
    51481 ttcatcgtgc tccagccccc agcgggccgc cagaacagat aaggcgccgt ctccggcgcc
    51541 tgtttcatca attaagcaga ttttcactga atgatcagcc cttaatgccg taatgctcca
    51601 gcatcgcatc cagctgcggt tcacgaccac ggaagcgttt gaacagatcc atcggctctt
    51661 ctgaaccgcc acggctcaga atgttgtcga ggaacgactg cccggtttca cggttgaaaa
    51721 tgccctcttc ctcaaagcgc gagaaagcat ctgccgccag tacgtcagcc cacaggtagc
    51781 tgtagtaacc tgccgcataa ccaccggcga aaatatggct gaaagcgtgc gggaaacggc
    51841 cccaggacgg agatggcacc acggcaacca gtttcttgat ttctgccaga gtttcgagga
    51901 tttttgcccc ctgatccggg cggaactcgg catgaaggcg gaaatcaaac aggccgaact
    51961 ccagctgacg cagaataaac agcgccgcct ggtagttctt cgccgccagc attttatcca
    52021 gcaactcttt cggcagcggt tcgccggttt catagtgacc agagataaac gccagcgcct
    52081 ccggctccca gcaccagttt tccataaact gactcggcag ttcgaccgca tcccacggca
    52141 caccgctgat accggaaaca ccagcggttt cgatgcgggt cagcatatgg tgcaggccgt
    52201 gaccgaactc gtggaacagg gtgatcactt cgtcgtgagt gaacagcgcc ggtttaccat
    52261 ttaccgggcg gttgaagtta caagtcaaat acgcgaccgg tttttgcaga gaaccatcag
    52321 ctttacgcat ctggcctacg cagtcatcca tccacgcccc gccgcgcttg ttttcacggg
    52381 catacagatc gaggtagaag ctgccgcgca gttcgttatt ttcgtcatac agttcgaaga
    52441 aacgtacatc cggatgccag acatcaacct ctttacgctc tttagcggtg atgccgtaaa
    52501 tacgtttcac cacttcaaac aggccgttaa ccgctttgtt ttccgggaag tacggacgca
    52561 gctgctcgtc gctgatgctg tagaggtgct gtttctgttt ttcgctgtag taagcgatat
    52621 cccacggctg caactcatcg acaccaaatt cggctttagc gaaggcacgc agttgctcca
    52681 gctctttttc gccttgcgga cgcgcgcgtt ttgccagatc ggttaagaaa tccagcacct
    52741 gctgcgggtt ttctgccatt ttagtggcaa gggatttaaa ggcgtagttt tcaaagccca
    52801 gcagttgcgc cagttcgtga cgcagcgcga ggatctcttc catcaccttg ctgttatccc
    52861 acttaccggc gttcgggcct tgatcggagg cgcgggtgct gtaagcgcga tacatctctt
    52921 cgcgcagagc ctggttgtcg cagtaggtca ttaccggcag gtagcttggg atatccagcg
    52981 tcagcaggta gccttccagc tctttcgctt cggcctgggc ttttgccgca gccagcgcgc
    53041 tttctggcat ccccgccagc cccgcttcgt cggtaacgag tttggtccag cccatcgtcg
    53101 catcgaggac gttgttgctg tactggttgc ccagttcaga aagacgggta gcaatttcgc
    53161 cgtaacgctg ctgtttctct ttcggcagac ctatgccaga gagttcaaaa tcacgcaggg
    53221 cgttatcaac cgctttcttc tgcgccgtgt tcagcgtggc gtaatgatcg ccatcgcgca
    53281 ggtcgcggta cgctttatac agcccttcat gttgccctac ccaggtgctg tattccgaca
    53341 gcagcggcag ggtttgctcg taggcttcac gcagttccgg gctatttttc accgagttca
    53401 ggtggctgac cggggagaag atacgcccca acacatcgtc cacttccgcc aacggctggc
    53461 agagattttc ccaggtgtac ggtgcccctt gcgctactac gcgcgccaca ttttcgcggc
    53521 aatcgttcag cgcctgagtc acggctggaa cgacatgttc cgggagaatt ttagaaaacg
    53581 gaggcaattc aaagggagtc agtaacggat tcgtcattcg cgcagtcctg gttaaagagg
    53641 ttaaggaagc gctcaacagg cgctttacat aatgtgtgta gcatggggtt aagtgtagtg
    53701 aatttcaatg agaaacgtta cgctttcgcg gcggcggcct cttttcggta tactgtcctg
    53761 atacgctttt gtgcgccccg aatacgggcc gatttttact taccggaaca cctttaccca
    53821 tgctcagtta tcgccacagc tttcacgctg gcaaccacgc cgacgtcctt aaacataccg
    53881 ttcagagcct gatcatcgag tcgctgaaag agaaagataa accgtttctc tatctcgata
    53941 cccacgcagg ggccgggcgt tatcagttag gcagcgaaca tgccgagcgt accggcgaat
    54001 atctcgaagg tatcgcccgt atctggcagc aggacgattt gcccgcagaa ctggaggcgt
    54061 acatcaatgt ggtaaaacac ttcaaccgta gcggtcagtt gcgttactac cccgggtcgc
    54121 cgttgattgc tcgccagcta ctgcgcgaac aagacagcct gcaactgacc gaactgcacc
    54181 cgagcgatta cccgttgctg cgttctgaat ttcagaaaga cagccgtgcg cgcgtcgaaa
    54241 aagccgacgg tttccagcag cttaaggcca aacttccgcc ggtttcccgc cgtggtttaa
    54301 tccttatcga cccgccgtat gaaatgaaaa ccgactatca ggcggtggtc agcgggatag
    54361 cagaaggtta caaacgtttc gccactggta cttacgcact gtggtatccg gtggtgctgc
    54421 gtcagcaaat taagcgcatg atccacgatc tggaagcgac cggtattcgc aaaattctgc
    54481 aaattgaact ggcggtactg ccagacagcg atcgccgtgg catgaccgct tccggcatga
    54541 ttgtgattaa cccgccgtgg aaactggaac agcagatgaa taacgtgctg ccgtggctgc
    54601 acagcaaact ggttccggca ggcaccgggc acgccaccgt aagctggatc gtgccggagt
    54661 aattgcagcc attgctggca cctattacgt ctcgcgctac aatcgcggta atcaacgata
    54721 aggacactct gtcatgacta aacactatga ttacatcgcc atcggcggcg gcagcggcgg
    54781 tatcgcctcc atcaaccgcg cggctatgta cggccagaaa tgcgcactga ttgaagccaa
    54841 agagctgggc ggcacctgcg tgaacgtcgg ctgcgtgccg aaaaaagtga tgtggcacgc
    54901 ggcgcaaatc cgtgaagcga tccatatgta cggccctgat tatggtttcg ataccaccat
    54961 caacaagttc aactgggaaa cgttgatcgc cagccgtacc gcctatatcg accgtattca
    55021 tacttcctat gaaaacgtgc tcggtaaaaa taacgttgat gtaatcaaag gctttgcccg
    55081 cttcgttgat gccaaaacgc tggaggtaaa cggcgaaacc atcacggccg atcatattct
    55141 gatcgccaca ggcggtcgtc cgagccaccc ggatattccg ggcgtggaat acggtattga
    55201 ttctgatggc ttcttcgccc ttcctgcttt gccagagcgc gtggcggttg ttggcgcggg
    55261 ttacatcgcc gttgagctgg cgggcgtgat taacggcctc ggcgcgaaaa cgcatctgtt
    55321 tgtgcgtaaa catgcgccgc tgcgcagctt cgacccgatg atttccgaaa cgctggtcga
    55381 agtgatgaac gccgaaggcc cgcagctgca caccaacgcc atcccgaaag cggtagtgaa
    55441 aaatgccgat ggtagcctga cgctggagct ggaagatggt cgcagtgaaa cggtggattg
    55501 cctgatttgg gcgattggtc gcgagcctgc caatgacaac atcaacctag aagccgctgg
    55561 cgttaaaact aacgaaaaag gctatatcgt cgtcgataaa tatcaaaaca ccaatattga
    55621 aggtatttac gcggtgggcg ataacacggg tgcagtggaa ctgacaccgg tagcagttgc
    55681 agcgggtcgc cgtctctctg aacgcctgtt taataacaag ccggatgagc atctggatta
    55741 cagcaacatt ccgaccgtgg tcttcagcca tccgccgatt ggtactgttg gtttaacgga
    55801 accgcaggcg cgcgagcagt acggcgacga tcaggtgaaa gtgtataaat cctctttcac
    55861 cgcgatgtat accgccgtca ccactcaccg ccagccgtgc cgcatgaagc tggtgtgcgt
    55921 tgggccagaa gagaagattg tcggtattca cggcattggc tttggtatgg acgaaatgtt
    55981 gcagggcttc gcggtggcgc tgaagatggg cgcaaccaaa aaagacttcg acaataccgt
    56041 cgccattcac ccaacggcgg cagaagagtt cgtgaccatg cgctaaatgt taaagggcta
    56101 agagtagtgt gctcttagcc cttaattacg tttccgctat cagttcagaa gctgaagcag
    56161 aaagcggatc agttccagca gcgcaattaa cgctccaaga acgatgattg ctttatcaat
    56221 cacccgtttt ctccatgcga tggagtgaga atgcatccgc ttactcatcc actgcctgtc
    56281 acggcgcatg tctcattgtt agatgacgac tatctcactc cggccagagc atcagttaac
    56341 ggcaccaccc gtacttctga ccaggacttt gaaagcgttt atgcgcatta ccagagtgaa
    56401 aatgcctcag agctaactgg ataatcatac agtacattca ggttataaaa ccagcatgtc
    56461 cttgcaatag tttcagtatg gtattagcat tgatgcgtta gatgatgact atctcactcc
    56521 agccagagcc accaactcag ggctgaagag taaaaaaacc gacgcaaagt cggttttttt
    56581 acatccggat tcggacaagg cttaatatga cgatgaccca gtgaaagtat ataaatcgtc
    56641 attgcgatat ataccgaagt gccccctccg ccagctgaag aaatcgctaa ttcttgcaat
    56701 gttagccact ggctaatagt attgaaccgt tagatgtggg cattctcact ccagccagag
    56761 ccaccaactc aggctgaaga gtaaaaaacc gacgcaaagt cggttttttt acgtcctgat
    56821 tcaaaccgcc tttcaaatga atagccaact caaaattcac acctattacc ttcctctgca
    56881 cttacacatt cgttaagtca tatatgtttt tgacttatcc gcttcgaaga gagacactac
    56941 ctgccacaat caggagcgca atatgtcatt tctgttaccc atccaattgt tcaaaattct
    57001 tgctgatgaa acccgtctgg gcatcgtttt actgctcagc gagctgggag agttatgcgt
    57061 ctgcgatctt tgtactgccc tcgatcagtc gcagcccaag atctcccgcc acctggcatt
    57121 gcttcgtgaa agcgggctat tgctggaccg caagcaaggt aagtgggttc attaccgctt
    57181 atcaccgcat attccagcat gggcggcgaa aattattgat gaggcctggc gatgtgaaca
    57241 ggaaaaggtt caggcgattg tccgcaacct ggctcgacaa aactgttccg gggacagtaa
    57301 gaacatttgc agttaaaaat ttagctaaac acatatgaat tttcagatgt gttttatccg
    57361 ggaggcatta tgttactggc aggcgctatc tttgtcctga ccatcgtatt ggttatctgg
    57421 cagccgaaag gtttaggcat cggctggagt gcaacgctcg gcgcagtact ggcgttagtt
    57481 acgggcgtgg ttcatccggg tgatattccg gtggtgtgga atatcgtctg gaacgcgacg
    57541 gctgcgttta tcgccgtcat tatcatcagc ctgctgctgg atgagtccgg cttttttgaa
    57601 tgggcggcgc tgcacgtctc acgctggggt aatggtcgtg gtcgcttgct gtttacctgg
    57661 attgtcctgc tcggtgctgc cgttgccgcc ctgtttgcca atgatggcgc ggcgcttatt
    57721 ttgacgccga ttgtcatcgc catgctgctg gctttaggat tcagtaaagg cacgacgctg
    57781 gcgttcgtga tggcggccgg attcattgcc gataccgcca gcctgccgct tattgtctcc
    57841 aacctggtga atatcgtttc cgctgatttc tttggcctcg gctttcgcga atacgcctcg
    57901 gtgatggtgc cggtggatat cgccgcgatt gttgccacgc tggtgatgct acatctctat
    57961 tttcgcaaag atattccgca gaactacgat atggcgctgc tgaaatctcc cgcagaagcg
    58021 atcaaagatc ctgctacgtt caaaactggc tgggttgttt tactgcttct gctggtggga
    58081 tttttcgtcc tggaaccgct cggcattccg gtgagcgcca ttgcagctgt gggcgcgctg
    58141 atattatttg tcgtcgctaa acgcggtcat gcgattaata cgggtaaagt cctgcgcggt
    58201 gccccctggc agattgtcat cttctcgctc ggcatgtatc tggtggttta tggcctgcgc
    58261 aatgccggat taacggaata tctttctggc gtactcaacg tgctggcgga taacggcctg
    58321 tgggccgcga cgctcggcac cggattcctc accgccttcc tctcttctat tatgaacaat
    58381 atgccgacgg tactggttgg cgcgttgtcc attgatggca gcacggcatc tggcgttatc
    58441 aaagaagcga tggtttatgc caatgtgatt ggctgcgatt tgggaccgaa aattacccca
    58501 attggtagcc tggctacgct actctggctg cacgtacttt cgcagaagaa tatgactatc
    58561 agctggggat attacttccg tacagggatt atcatgaccc tgcctgtgct gtttgtgacg
    58621 ctggctgcgc tggcgctacg tctctctttc actttgtaat gagatactga tatgagcaac
    58681 attaccattt atcacaaccc ggcctgcggc acgtcgcgta atacgctgga gatgatccgc
    58741 aacagcggca cagaaccgac cattatccat tatctggaaa ctccgccaac gcgcgatgaa
    58801 ctggtcaaac tcattgccga tatggggatt tccgtacgcg cgctgctgcg taaaaacgtc
    58861 gaaccgtatg aggagctggg ccttgcggaa gataaattta ctgacgatcg gttaatcgac
    58921 tttatgcttc agcacccgat tctgattaat cgcccgattg tggtgacgcc gctgggaact
    58981 cgcctgtgcc gcccttcaga agtggtgctg gaaattctgc cagatgcgca aaaaggcgca
    59041 ttctccaagg aagatggcga gaaagtggtt gatgaagcgg gtaagcgcct gaaataaagc
    59101 ggcgatatcc actgccgata gttggtaaag tgcgctttgt ttatgtcgga tgcgacgcta
    59161 acgcgtctta tccgacctac aaaaccgtgc aaattcaata tatggcatga aatatgtaga
    59221 gcgtagcgca tcaggcaatt ttgctattta cgcagcactc ctcgcgtaag aattcaatta
    59281 tcgcttccag cattgcgtat tgcgaaacac acatcagcgt ccgactctca cggttttgtt
    59341 tgaccagtcc taccgatacc agtgctgata tatggtggct gagtgtggag ccaggtatcc
    59401 caagctgctt ttgcaactca ccgaccggca acccttgttc cccggctttg acaagatgtt
    59461 taaatatgaa caaacgggtc gggtgaccta gctctttcag cgctttagcg acttcttcca
    59521 actgcatatg acacccctct gtctggttga tatttcgata ataccagaaa tatcttgatc
    59581 tgaatttcat ttcgattatt ctggaaatat agatttatcc aatgaggtta cgtatgagta
    59641 gttggcttgc gatgctgcaa gatgccgcag agatgtttgt gtttctcgcc gtcgagcttt
    59701 cgttgctgtt tatagtgatt agtgccggtg tcagcctgat aagacaaaag gtgccagacc
    59761 ataaaatcca gcagatgatg ggggccagaa aagggagagg ctatctcctg gctgctctgt
    59821 tgggagccgt taccccgttc tgtagttgct cgacaatacc catgttacgt ggattgttgt
    59881 cagcgaaagc cgggt
//
LOCUS       DAQSWQ010000012        58387 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.012, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000012 DAQSWQ010000000
VERSION     DAQSWQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.012"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..160
                     /locus_tag="R8G18_000768"
     CDS             <1..160
                     /locus_tag="R8G18_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEE9794553.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="R8G18_000769"
     CDS             complement(198..1259)
                     /locus_tag="R8G18_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEE9794554.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="R8G18_000770"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="R8G18_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HEE9794555.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="R8G18_000771"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="R8G18_000771"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEE9794556.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4199)
                     /gene="hda"
                     /locus_tag="R8G18_000772"
     CDS             complement(3453..4199)
                     /gene="hda"
                     /locus_tag="R8G18_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HEE9794557.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="R8G18_000773"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="R8G18_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEE9794558.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="R8G18_000774"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="R8G18_000774"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEE9794559.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="R8G18_000775"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="R8G18_000775"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEE9794560.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="R8G18_000776"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="R8G18_000776"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEE9794561.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="R8G18_000777"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="R8G18_000777"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEE9794562.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="R8G18_000778"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="R8G18_000778"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEE9794563.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="R8G18_000779"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="R8G18_000779"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HEE9794564.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14497..14649)
                     /locus_tag="R8G18_000780"
     CDS             complement(14497..14649)
                     /locus_tag="R8G18_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794565.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14667..14858
                     /locus_tag="R8G18_000781"
     CDS             14667..14858
                     /locus_tag="R8G18_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HEE9794566.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15169..15687
                     /locus_tag="R8G18_000782"
     CDS             15169..15687
                     /locus_tag="R8G18_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEE9794567.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15703..16242
                     /locus_tag="R8G18_000783"
     CDS             15703..16242
                     /locus_tag="R8G18_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HEE9794568.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="R8G18_000784"
     CDS             complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="R8G18_000784"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEE9794569.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="R8G18_000785"
     CDS             complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="R8G18_000785"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEE9794570.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19611..20981
                     /gene="xseA"
                     /locus_tag="R8G18_000786"
     CDS             19611..20981
                     /gene="xseA"
                     /locus_tag="R8G18_000786"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEE9794571.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20978..21193)
                     /locus_tag="R8G18_000787"
     CDS             complement(20978..21193)
                     /locus_tag="R8G18_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEE9794572.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21262..22734)
                     /gene="der"
                     /locus_tag="R8G18_000788"
     CDS             complement(21262..22734)
                     /gene="der"
                     /locus_tag="R8G18_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEE9794573.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="R8G18_000789"
     CDS             complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="R8G18_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEE9794574.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="R8G18_000790"
     CDS             complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="R8G18_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HEE9794575.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="R8G18_000791"
     CDS             complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="R8G18_000791"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEE9794576.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="R8G18_000792"
                     /gene_synonym="gcpE"
     CDS             complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="R8G18_000792"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEE9794577.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="R8G18_000793"
     CDS             complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="R8G18_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HEE9794578.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="R8G18_000794"
     CDS             complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="R8G18_000794"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HEE9794579.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="R8G18_000795"
     CDS             complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="R8G18_000795"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEE9794580.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="R8G18_000796"
     CDS             complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="R8G18_000796"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HEE9794581.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="R8G18_000797"
     CDS             complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="R8G18_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HEE9794582.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37872..38717
                     /gene="sseA"
                     /locus_tag="R8G18_000798"
     CDS             37872..38717
                     /gene="sseA"
                     /locus_tag="R8G18_000798"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEE9794583.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="R8G18_000799"
     CDS             complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="R8G18_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HEE9794584.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="R8G18_000800"
     CDS             complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="R8G18_000800"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HEE9794585.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="R8G18_000801"
     CDS             complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="R8G18_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEE9794586.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="R8G18_000802"
     CDS             complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="R8G18_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEE9794587.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="R8G18_000803"
     CDS             complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="R8G18_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEE9794588.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="R8G18_000804"
     CDS             complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="R8G18_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HEE9794589.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="R8G18_000805"
     CDS             complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="R8G18_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEE9794590.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="R8G18_000806"
     CDS             complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="R8G18_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEE9794591.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="R8G18_000807"
     CDS             complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="R8G18_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEE9794592.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="R8G18_000808"
     CDS             complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="R8G18_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEE9794593.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="R8G18_000809"
     CDS             complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="R8G18_000809"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HEE9794594.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48470..49273
                     /gene="suhB"
                     /locus_tag="R8G18_000810"
     CDS             48470..49273
                     /gene="suhB"
                     /locus_tag="R8G18_000810"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HEE9794595.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49418..50272
                     /locus_tag="R8G18_000811"
     CDS             49418..50272
                     /locus_tag="R8G18_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEE9794596.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50463..51743
                     /gene="csiE"
                     /locus_tag="R8G18_000812"
     CDS             50463..51743
                     /gene="csiE"
                     /locus_tag="R8G18_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HEE9794597.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51735..52874)
                     /locus_tag="R8G18_000813"
     CDS             complement(51735..52874)
                     /locus_tag="R8G18_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HEE9794598.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="R8G18_000814"
     CDS             complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="R8G18_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HEE9794599.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54060..55421
                     /locus_tag="R8G18_000815"
     CDS             54060..55421
                     /locus_tag="R8G18_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEE9794600.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55418..55936
                     /locus_tag="R8G18_000816"
     CDS             55418..55936
                     /locus_tag="R8G18_000816"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HEE9794601.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55936..56256
                     /locus_tag="R8G18_000817"
     CDS             55936..56256
                     /locus_tag="R8G18_000817"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HEE9794602.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56253..57065
                     /gene="hcaB"
                     /locus_tag="R8G18_000818"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56253..57065
                     /gene="hcaB"
                     /locus_tag="R8G18_000818"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HEE9794603.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57075..58277
                     /locus_tag="R8G18_000819"
     CDS             57075..58277
                     /locus_tag="R8G18_000819"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HEE9794604.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14269 a        15788 c        14570 g        13760 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gcggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 aataaccctg attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc
    14521 attgtaaata attaaattaa cttccataac attaaaatat gtatccactg acgttttttt
    14581 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc
    14641 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
    14701 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
    14761 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
    14821 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac
    14881 agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag
    14941 atattctttc atcttaaatt ttacgtcttt gtcctgactg atgtttatcc tgtttggctg
    15001 cggaataaat ataaaattaa tatatatgtt gtaatgatat atttttataa attattccct
    15061 gcgtgaattt taataaattt aatctatccc tttatacgca atacatttac tttcctcttt
    15121 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
    15181 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
    15241 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
    15301 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
    15361 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
    15421 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
    15481 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
    15541 ttaacatata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
    15601 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
    15661 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
    15721 tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
    15781 tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
    15841 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
    15901 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
    15961 ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
    16021 agcttagaga ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg cgttaagcga
    16081 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
    16141 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
    16201 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
    16261 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
    16321 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
    16381 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
    16441 aatcgtacgg cagacgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
    16501 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
    16561 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
    16621 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
    16681 cacgaacgcc aaggcctggt cccgggaatg ggtgacggta caacatgtcg tacggcaggc
    16741 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
    16801 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
    16861 ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
    16921 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac
    16981 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
    17041 cttctgccgg aacgtgaaca atgttcagac caaagtgatc gccaaacata tccagaacct
    17101 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
    17161 cgatagcgcg gtgcagtagc attgcggtta cggaggaatc cacgccacca gagaggccga
    17221 ggatgacttt atcgtcgcct acctgctcac ggatgcgggc tacagcatcg tcgataattt
    17281 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
    17341 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
    17401 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
    17461 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
    17521 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg acttcaacct
    17581 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
    17641 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
    17701 gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga ataatgccgc
    17761 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
    17821 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtccagaa
    17881 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
    17941 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
    18001 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
    18061 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
    18121 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
    18181 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
    18241 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
    18301 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
    18361 catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
    18421 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
    18481 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
    18541 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
    18601 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
    18661 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
    18721 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
    18781 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
    18841 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
    18901 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
    18961 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
    19021 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
    19081 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
    19141 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
    19201 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
    19261 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
    19321 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
    19381 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
    19441 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
    19501 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
    19561 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
    19621 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
    19681 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
    19741 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
    19801 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
    19861 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
    19921 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
    19981 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
    20041 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
    20101 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
    20161 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
    20221 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
    20281 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
    20341 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
    20401 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
    20461 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
    20521 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
    20581 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
    20641 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
    20701 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
    20761 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
    20821 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
    20881 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa
    20941 acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
    21001 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
    21061 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
    21121 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
    21181 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
    21241 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
    21301 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
    21361 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
    21421 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
    21481 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
    21541 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
    21601 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
    21661 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
    21721 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
    21781 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
    21841 aataccttcg cgcgcatcaa tcactaacat caccacgttg gtatcttcaa tggcctgcaa
    21901 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
    21961 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
    22021 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
    22081 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
    22141 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
    22201 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
    22261 cacacgctcc agcagactta atacgccacg accgtgagat gcggcgatcg ggtagatttc
    22321 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
    22381 gtttgccacc aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa tcgcttcatc
    22441 tgccggcatc aagcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
    22501 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
    22561 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
    22621 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
    22681 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
    22741 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
    22801 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
    22861 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
    22921 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
    22981 gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac
    23041 cggcgaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc
    23101 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
    23161 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
    23221 aagcgccgtc aggttaccat tataggccag agcgaaaaca acgccgttaa caacgacggg
    23281 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
    23341 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
    23401 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
    23461 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
    23521 ctgaccgtta ctagtgtgga ttaacaccag accgtcgctg accaccgggc gagaaagtgc
    23581 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
    23641 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
    23701 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
    23761 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
    23821 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
    23881 agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
    23941 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
    24001 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
    24061 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
    24121 atgcactacg cgcaccttgc ttatcacctt tgctcaacaa tgctttacca cgcaggtcgg
    24181 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
    24241 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
    24301 catcactcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
    24361 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
    24421 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
    24481 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
    24541 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
    24601 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
    24661 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
    24721 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
    24781 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
    24841 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
    24901 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
    24961 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
    25021 acgttcgagg cccatcgcaa aaccgactgc cggtgttgca cgaccgccca gttgttccac
    25081 cagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgaggc tgttagtcac
    25141 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    25201 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    25261 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    25321 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    25381 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    25441 cagcgaacca atagagttca gctcaagagt tacgtgttcg aagataccca gcgcacgcca
    25501 ccagcgggca gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    25561 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    25621 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    25681 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    25741 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    25801 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    25861 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    25921 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    25981 gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca tacacgggaa
    26041 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    26101 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    26161 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    26221 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    26281 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    26341 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttaat ccctcgcgaa
    26401 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    26461 gccagcgata cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc taaaccaatg
    26521 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    26581 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    26641 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atgacgcatg
    26701 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    26761 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    26821 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    26881 ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    26941 acgttaaccc gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    27001 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    27061 acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    27121 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    27181 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    27241 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    27301 actggatctg tactgcggct ggcgcaccaa ttttcagttt atacggtgcc tggccggtta
    27361 aattcaaatt accgtcttta cgctgcatcc cgctaaacaa ttttttaccg gtagcatcag
    27421 tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    27481 gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc gttgttgctg
    27541 ccgttggtac cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    27601 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    27661 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    27721 tatttaacgg gacactctgc ccctgctcgc tattactgct cagttctgcc gaagattgat
    27781 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    27841 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccatcacgtt
    27901 ttttgcggcg tttaccgagg gaaaaactct gcattggcgc aacttttgca gcccgtagtg
    27961 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    28021 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    28081 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    28141 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    28201 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    28261 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    28321 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    28381 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    28441 caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa
    28501 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    28561 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcaa tatcatcacc
    28621 acgagtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    28681 gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag
    28741 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    28801 gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat tggcgttgga
    28861 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    28921 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    28981 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    29041 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    29101 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattggccg
    29161 ctgaccggtg acttttgctg cgccgacgat tttcgccgcc cgccacacct ggccgataat
    29221 ttccgacacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    29281 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    29341 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    29401 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    29461 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    29521 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    29581 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    29641 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    29701 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    29761 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    29821 cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg
    29881 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    29941 ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    30001 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    30061 tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg
    30121 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    30181 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    30241 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    30301 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    30361 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    30421 ttgccccacg tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    30481 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    30541 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    30601 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatggcgg
    30661 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    30721 ttccagcggt agcggccaga catttaccat ttcttgcttc gcctgcgggc agtcagcggc
    30781 gacgcgctgc cctttgtcat ccagccagat ggggaagcga atgccattaa tcccttcctg
    30841 ctctgacagt aacagcgttg gcggctggct gccatccagc agccaggttg ccaggcggcg
    30901 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    30961 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    31021 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    31081 gccgtcaggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    31141 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    31201 ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc
    31261 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    31321 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    31381 agcgccgccg agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat ataacggcaa
    31441 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    31501 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    31561 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    31621 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    31681 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    31741 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    31801 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    31861 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    31921 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    31981 ccttgactct tttacctgct cacgggacca cacaccttgc gcagccatcc gttcgagcac
    32041 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    32101 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    32161 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    32221 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    32281 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    32341 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    32401 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    32461 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    32521 gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc
    32581 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    32641 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    32701 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    32761 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    32821 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    32881 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    32941 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc
    33001 cagacccaca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    33061 ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    33121 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    33181 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgttgctcac
    33241 ctgcaaggtg acaagttgat cgctattcag attgctgttt tgcgctttct cgcctgtcag
    33301 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    33361 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    33421 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag
    33481 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    33541 ctcatcacta tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac cacgcgtcgc
    33601 atcacccatg gttttcagcg caatgccaag ttgcagcagc ggtaaaccag aagcggcatc
    33661 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    33721 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    33781 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    33841 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    33901 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    33961 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    34021 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    34081 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    34141 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    34201 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    34261 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    34321 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    34381 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    34441 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    34501 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    34561 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    34621 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    34681 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    34741 taccgacagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    34801 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    34861 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    34921 ccacggatct ggcgtaacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    34981 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    35041 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    35101 ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    35161 tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat tccaggtttt
    35221 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag
    35281 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg
    35341 caacttaatg gtatcgccgg ggcgataact ggctttatct agtttcagcg tgacacggtc
    35401 gggtcgcact gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag cccaaaaacg
    35461 cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    35521 cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat
    35581 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    35641 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    35701 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    35761 caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg gacggagccc
    35821 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    35881 cgattccagc aaactgccct ggaaaataac ctgcaatggg gaatgcgttt ccttccactg
    35941 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    36001 cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    36061 agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc
    36121 accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg taagcggggt
    36181 tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga cgtggaaatc
    36241 ccacatccgg tactggttat cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc
    36301 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgtt
    36361 cctgagtacc tgcccgtcag gtttaatcac gtctaacttg atgggttgat cgggcaatgc
    36421 tttaccgtct gcatcgcgca gtaaaccatt gaggattacc gtttcacctg ggcgataaag
    36481 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    36541 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    36601 tgccagcagt aatgccgcat ttttatcatt ttccagctgc acatgcccct gagcgtcact
    36661 ggttgcctga gtcagagtct gtcctttctc atttaataaa gagacttcaa ttccttgctg
    36721 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    36781 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    36841 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    36901 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    36961 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    37021 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    37081 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    37141 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    37201 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    37261 caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat ctgacagctc
    37321 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    37381 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    37441 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    37501 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    37561 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    37621 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    37681 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    37741 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    37801 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    37861 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    37921 atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag gaagatcgta
    37981 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    38041 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    38101 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    38161 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    38221 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    38281 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    38341 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    38401 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    38461 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    38521 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    38581 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    38641 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    38701 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    38761 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    38821 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    38881 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    38941 gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc
    39001 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    39061 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa
    39121 ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc
    39181 ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttttgttt aaagtcacgc
    39241 aaaaaaccac cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta
    39301 attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta
    39361 tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg
    39421 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    39481 ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    39541 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    39601 ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc
    39661 ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg
    39721 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg
    39781 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta
    39841 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt
    39901 agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    39961 tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa
    40021 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    40081 aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
    40141 agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc
    40201 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    40261 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    40321 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    40381 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    40441 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    40501 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    40561 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    40621 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    40681 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    40741 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    40801 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct
    40861 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt
    40921 ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    40981 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    41041 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    41101 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    41161 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    41221 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    41281 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    41341 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    41401 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    41461 ttttccgcta aatgattcgc gttgcagaaa ggcggcaagt gagtgaagcc ccaggagcat
    41521 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    41581 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg
    41641 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
    41701 gtgaatcgaa ccgttttcgg atcaagatca ggatacgcat cgtacagtgc ttcgccaatt
    41761 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    41821 tgatagtgta acgcgggatt tcgactacta aatcttcgtc ggtaacgcgt gcctggcagc
    41881 ttaaacggct ttcgggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    41941 agctttctgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    42001 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    42061 tttcaccgct attagcttcc agaacagcgc catcaggaca gagatcctga tgaggcaaaa
    42121 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    42181 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    42241 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    42301 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    42361 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    42421 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    42481 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    42541 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accaccagcc
    42601 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    42661 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    42721 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
    42781 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
    42841 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    42901 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    42961 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
    43021 cccgcgtctt taagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    43081 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    43141 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    43201 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    43261 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
    43321 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
    43381 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    43441 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    43501 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    43561 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    43621 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    43681 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    43741 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    43801 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    43861 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    43921 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    43981 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    44041 actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    44101 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    44161 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    44221 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    44281 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    44341 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    44401 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    44461 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagaaggtga agtaatccat
    44521 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    44581 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    44641 ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt
    44701 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    44761 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    44821 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
    44881 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    44941 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    45001 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    45061 agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    45121 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    45181 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    45241 tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    45301 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    45361 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    45421 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    45481 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    45541 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    45601 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    45661 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    45721 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    45781 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat
    45841 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    45901 cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc
    45961 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    46021 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    46081 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    46141 aacaaggatg gtgtcatcac gcatcgctgc ttcaagttct ttcagatcga taatgccgtt
    46201 acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    46261 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
    46321 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    46381 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    46441 ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat
    46501 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    46561 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    46621 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    46681 tctaatatct tgttatcttt aacctaactg tttcgggttt cagcaagttg acaaacttac
    46741 gaatctccgg gagcttacat aagtaagtga ccggagtgag tatgtgcagt caacgcagat
    46801 gcaacacgaa acaggacggt taaattaagc gcgtaactta acgtcgatcg cgtcttgtat
    46861 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
    46921 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
    46981 acgccacagc gcgtgggtca ggcatttatc gccgccctgg caaccgcctt taccctgaca
    47041 acgggtagca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
    47101 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
    47161 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
    47221 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
    47281 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
    47341 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
    47401 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
    47461 cattttttta tcgtggcgtt tgtacctgtt gtccttccag accatgggcg ggttgagggc
    47521 aattattcgc attagcgctg gcctcttccc ctcaccctaa ccctctcccc gaaggggcga
    47581 ggggaccgtc cgtgcattta tctggcttag ccctcaggcc ttattacctt tattctgctg
    47641 ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    47701 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    47761 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    47821 atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat
    47881 acgcacttca taggcaataa cctgaaccgc cattgccaag ttcagcgaac tgtattccgg
    47941 gtttgccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    48001 gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    48061 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    48121 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    48181 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg
    48241 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    48301 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    48361 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    48421 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    48481 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    48541 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    48601 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttactcac agcacaccat
    48661 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    48721 tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    48781 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    48841 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac
    48901 cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta
    48961 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    49021 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    49081 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg
    49141 cggcatcgtc agcgacttca ccggtggtca taactacatg ctgaccggta acatcgttgc
    49201 tggtaacccg cgcgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    49261 tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    49321 tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    49381 cgattaatgg atgataaaga agtcacaagg aaaggttatg gcgctgccag tgaacaaacg
    49441 cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    49501 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    49561 ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc
    49621 ttcttcgacg ggccctgctg acaatgccat cgcaaccatc attcatgctc acggcaatgc
    49681 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    49741 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    49801 gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    49861 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    49921 ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    49981 catcgccaat cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    50041 aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    50101 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagaga caaaacggtt
    50161 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta
    50221 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    50281 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    50341 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    50401 tcgccatctc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    50461 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    50521 tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta
    50581 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    50641 gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag
    50701 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt
    50761 catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    50821 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    50881 tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    50941 tgctgcaaca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    51001 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    51061 tccagcctgt accgcctgat gagccactgt ttatggcatt acttttttcc atgttgcggg
    51121 ttcccgatcc attgcgtgat gcgcatcagc gagacagaca attgcgtcag tctatcaaac
    51181 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat
    51241 gcgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    51301 ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc
    51361 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg
    51421 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    51481 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    51541 gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga
    51601 caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct
    51661 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    51721 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    51781 gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    51841 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    51901 cggatgactt cgctaccctg gcgaacagca atatagcgca tggcggccag gtggcagacc
    51961 gtgaaggtgc cgcaatgcaa aatttgcacc actatcaacc acggcaacgc cgtagttgct
    52021 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgagca
    52081 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    52141 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    52201 ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc
    52261 cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcgactt
    52321 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    52381 aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    52441 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    52501 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    52561 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    52621 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    52681 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    52741 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    52801 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    52861 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    52921 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    52981 acaagcacta aaaaactgcg atccgcgacg catgttgcaa aaataatgcg taattatgcc
    53041 gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    53101 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    53161 aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    53221 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    53281 tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc
    53341 ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg cggtgatctc tttttcatgt
    53401 gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag ctccagataa
    53461 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    53521 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    53581 ctgagacgaa acattggtaa tacttttggc aataaattca cttccgccga tggcacaaag
    53641 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    53701 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    53761 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    53821 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    53881 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    53941 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccagca
    54001 acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata
    54061 tgaccacacc ctcagattta aacatttacc aactgattga tacccaaaat ggtcgggtca
    54121 ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc
    54181 gttgctggtt atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca
    54241 cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt
    54301 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    54361 gcgcctttac ctgtccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    54421 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg
    54481 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    54541 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    54601 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga
    54661 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    54721 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    54781 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    54841 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    54901 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    54961 tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctcaac ggcactgcca
    55021 cgctccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta
    55081 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    55141 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    55201 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    55261 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    55321 ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc
    55381 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    55441 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    55501 atttcgtgac tggctggagc agctcgacaa agagattcgt tacaccatgc gcaccacagt
    55561 taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa
    55621 tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    55681 agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    55741 catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg
    55801 cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa
    55861 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    55921 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    55981 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    56041 tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa
    56101 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    56161 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    56221 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    56281 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    56341 gcgcgcaagt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat
    56401 ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg
    56461 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca
    56521 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    56581 acgagctgtt taacgtcaac gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg
    56641 cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc aggtatcctg
    56701 gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac
    56761 tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca
    56821 gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga
    56881 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    56941 cggggccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    57001 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc
    57061 tttaaggaag cacgatgaaa gaaaaaacga tcattattgt cggtggcggg caagcggcgg
    57121 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    57181 atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt
    57241 ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc
    57301 attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg
    57361 gcgaaagctg gcactgggat cagcttttta tagcaatcgg cgcggcagct cgaccgctgc
    57421 cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca
    57481 gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg
    57541 gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg
    57601 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    57661 accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    57721 aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg
    57781 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg
    57841 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    57901 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    57961 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc
    58021 cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc gatatgcgtg
    58081 gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc
    58141 aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg gcgtgagatt cgcccaattc
    58201 gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat gagaacatcg
    58261 cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg gcgtggcgag
    58321 gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg gttatgaaca
    58381 cactacg
//
LOCUS       DAQSWQ010000013        57430 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.013, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000013 DAQSWQ010000000
VERSION     DAQSWQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.013"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            147..1490
                     /gene="argG"
                     /locus_tag="R8G18_000820"
     CDS             147..1490
                     /gene="argG"
                     /locus_tag="R8G18_000820"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEE9794605.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1498..3123)
                     /locus_tag="R8G18_000821"
     CDS             complement(1498..3123)
                     /locus_tag="R8G18_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEE9794606.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3582..3668)
                     /locus_tag="R8G18_000822"
     tRNA            complement(3582..3668)
                     /locus_tag="R8G18_000822"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:Leu,seq:gag)
     gene            complement(3683..4015)
                     /gene="secG"
                     /locus_tag="R8G18_000823"
     CDS             complement(3683..4015)
                     /gene="secG"
                     /locus_tag="R8G18_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEE9794607.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="R8G18_000824"
     CDS             complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="R8G18_000824"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEE9794608.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5573..6421)
                     /gene="folP"
                     /locus_tag="R8G18_000825"
     CDS             complement(5573..6421)
                     /gene="folP"
                     /locus_tag="R8G18_000825"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEE9794609.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="R8G18_000826"
     CDS             complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="R8G18_000826"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEE9794610.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="R8G18_000827"
     CDS             complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="R8G18_000827"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HEE9794611.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9300..9593
                     /gene="yhbY"
                     /locus_tag="R8G18_000828"
     CDS             9300..9593
                     /gene="yhbY"
                     /locus_tag="R8G18_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEE9794612.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9749..10225)
                     /gene="greA"
                     /locus_tag="R8G18_000829"
     CDS             complement(9749..10225)
                     /gene="greA"
                     /locus_tag="R8G18_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEE9794613.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10473..11906
                     /gene="dacB"
                     /locus_tag="R8G18_000830"
     CDS             10473..11906
                     /gene="dacB"
                     /locus_tag="R8G18_000830"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HEE9794614.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="R8G18_000831"
     CDS             complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="R8G18_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEE9794615.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13280..14245)
                     /locus_tag="R8G18_000832"
     CDS             complement(13280..14245)
                     /locus_tag="R8G18_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEE9794616.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="R8G18_000833"
     CDS             complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="R8G18_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEE9794617.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="R8G18_000834"
     CDS             complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="R8G18_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEE9794618.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15220..16191
                     /gene="ispB"
                     /locus_tag="R8G18_000835"
     CDS             15220..16191
                     /gene="ispB"
                     /locus_tag="R8G18_000835"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEE9794619.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="R8G18_000836"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="R8G18_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HEE9794620.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="R8G18_000837"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="R8G18_000837"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEE9794621.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="R8G18_000838"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="R8G18_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HEE9794622.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="R8G18_000839"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="R8G18_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEE9794623.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="R8G18_000840"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="R8G18_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEE9794624.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="R8G18_000841"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="R8G18_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEE9794625.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="R8G18_000842"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="R8G18_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEE9794626.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="R8G18_000843"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="R8G18_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HEE9794627.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="R8G18_000844"
     CDS             21785..22762
                     /locus_tag="R8G18_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEE9794628.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="R8G18_000845"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="R8G18_000845"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HEE9794629.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="R8G18_000846"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="R8G18_000846"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HEE9794630.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="R8G18_000847"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="R8G18_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEE9794631.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="R8G18_000848"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="R8G18_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HEE9794632.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="R8G18_000849"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="R8G18_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEE9794633.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="R8G18_000850"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="R8G18_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEE9794634.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="R8G18_000851"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="R8G18_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HEE9794635.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="R8G18_000852"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="R8G18_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEE9794636.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="R8G18_000853"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="R8G18_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEE9794637.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="R8G18_000854"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="R8G18_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HEE9794638.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="R8G18_000855"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="R8G18_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HEE9794639.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="R8G18_000856"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="R8G18_000856"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEE9794640.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="R8G18_000857"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="R8G18_000857"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HEE9794641.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="R8G18_000858"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="R8G18_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HEE9794642.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="R8G18_000859"
     CDS             complement(34631..35560)
                     /locus_tag="R8G18_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEE9794643.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="R8G18_000860"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="R8G18_000860"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEE9794644.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="R8G18_000861"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="R8G18_000861"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HEE9794645.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42789
                     /locus_tag="R8G18_000862"
     CDS             42310..42789
                     /locus_tag="R8G18_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HEE9794646.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42806..43111
                     /locus_tag="R8G18_000863"
     CDS             42806..43111
                     /locus_tag="R8G18_000863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEE9794647.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /locus_tag="R8G18_000864"
     CDS             complement(43187..43651)
                     /locus_tag="R8G18_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEE9794648.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="R8G18_000865"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="R8G18_000865"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HEE9794649.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="R8G18_000866"
     CDS             complement(44520..45209)
                     /locus_tag="R8G18_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEE9794650.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="R8G18_000867"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="R8G18_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HEE9794651.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="R8G18_000868"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="R8G18_000868"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEE9794652.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="R8G18_000869"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="R8G18_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HEE9794653.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49043..50365
                     /gene="dcuC"
                     /locus_tag="R8G18_000870"
     CDS             49043..50365
                     /gene="dcuC"
                     /locus_tag="R8G18_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HEE9794654.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="R8G18_000871"
     CDS             complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="R8G18_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEE9794655.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="R8G18_000872"
     CDS             complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="R8G18_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HEE9794656.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="R8G18_000873"
     CDS             complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="R8G18_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEE9794657.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="R8G18_000874"
     CDS             complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="R8G18_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEE9794658.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="R8G18_000875"
     CDS             complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="R8G18_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEE9794659.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54320..54718
                     /locus_tag="R8G18_000876"
     CDS             54320..54718
                     /locus_tag="R8G18_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HEE9794660.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54872..56239
                     /gene="degQ"
                     /locus_tag="R8G18_000877"
     CDS             54872..56239
                     /gene="degQ"
                     /locus_tag="R8G18_000877"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HEE9794661.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56329..57396
                     /gene="degS"
                     /locus_tag="R8G18_000878"
     CDS             56329..57396
                     /gene="degS"
                     /locus_tag="R8G18_000878"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HEE9794662.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14258 a        15304 c        14261 g        13607 t
ORIGIN      
        1 aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta
       61 aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat
      121 cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc
      181 aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc
      241 gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg
      301 attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg
      361 actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata
      421 acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta
      481 ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct
      541 ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc
      601 agatttacaa accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga
      661 tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact
      721 ccacagactc caacatgctt ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact
      781 ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga
      841 tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta
      901 aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg
      961 gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt
     1021 acgaagctcc ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc
     1081 acaacgaaga caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt
     1141 accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg caacgctggg
     1201 ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa
     1261 tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag
     1321 gcgactcggt gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata
     1381 tcactgatac ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg
     1441 ccacttcagg cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca
     1501 accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat
     1561 cacataatcg aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc
     1621 aatcacttcc tccagcgttt tcggttgttc cggctttgtt acccccatcc acgcattaat
     1681 aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtacgat ccacgccatc
     1741 cccaagaacg gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc
     1801 cctgcctcca tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc
     1861 aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact
     1921 atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac
     1981 ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac
     2041 aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa
     2101 ttcatcaaat cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc
     2161 tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg
     2221 attagccata ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc
     2281 agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgattgatt
     2341 aaacagtttg atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc
     2401 atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat
     2461 acctgtatcc ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct
     2521 ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg tataggtagc
     2581 aaatcgcgac gctagtatat atggactgaa tgctttttta ttttttgcat ctttataagc
     2641 aaattgacaa gcagaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac
     2701 tttttttgtc ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa
     2761 aaaagcaaaa gcacatagat aagggatata catccccagc attttgacaa cttcatccgg
     2821 atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca
     2881 tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag
     2941 caggcgaagc ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag
     3001 caatcgctga accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat
     3061 aacacaaaga tacaacaacc aatgagattt aaaacttcta gcaaatttgt tgaatactgt
     3121 catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtt caattttata
     3181 tcagacatgt ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt
     3241 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
     3301 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
     3361 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
     3421 agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
     3481 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
     3541 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     3601 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     3661 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
     3721 ctggctgagt ttgttcggtt ttcgccggtg cactcagatt ttcccattcg ctacctttat
     3781 tggttttgtt gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca
     3841 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     3901 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     3961 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
     4021 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     4081 atctttccca ctgaagtggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     4141 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     4201 aaaggcgaca atcgccgcct ttttagccag ttatttaccg ttttaaacgg cttttactgc
     4261 atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt ctaccatcac
     4321 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     4381 cgcttcaact tcggcggtca cagctttaac tgactcatgc tcaagcggat cgccgctacc
     4441 tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     4501 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     4561 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     4621 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     4681 tggaattcct aactgtttca acgccagctc aagccccatg ttactcatca atgtacccac
     4741 agcaccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     4801 atcaccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     4861 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     4921 ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga tagcgataac
     4981 gttcgccccc agttcacgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     5041 atccaccaca atcttcagtt cgctgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     5101 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     5161 aacacagctg atctcttttt ccatttccgc ttcgatggcc tcttctaccg catccggtag
     5221 tttggtgccg tcgatagaga aaaatttaat gccattatcg tagaacgggt tatgcgatgc
     5281 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     5341 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     5401 tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc
     5461 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     5521 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     5581 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     5641 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     5701 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     5761 ttcgtgacat acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     5821 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     5881 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     5941 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     6001 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     6061 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     6121 ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     6181 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     6241 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     6301 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     6361 ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca aagagtttca
     6421 tggtgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     6481 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     6541 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     6601 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     6661 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     6721 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     6781 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     6841 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     6901 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgcgcca ggttggtcgc
     6961 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     7021 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     7081 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     7141 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     7201 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     7261 acccatcatg attttatctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     7321 gccacgagca gcgaacagtg ccgcttcgtt taccaggttc gccaggtcag caccggagaa
     7381 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     7441 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     7501 acggtcgaaa cggccaggac gcagcagggc cgggtcaaga acgtccggac ggttagtcgc
     7561 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     7621 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     7681 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     7741 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     7801 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     7861 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     7921 gctcggctcg cgcagatatt caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     7981 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     8041 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     8101 cacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     8161 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     8221 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     8281 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     8341 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     8401 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     8461 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     8521 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     8581 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     8641 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     8701 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     8761 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     8821 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     8881 gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     8941 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     9001 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     9061 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     9121 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     9181 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     9241 tatatgggag atggcggtag aatgacccat tttcaatccc aacgtaagca aaaaatacga
     9301 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     9361 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     9421 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct
     9481 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     9541 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     9601 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     9661 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     9721 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     9781 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     9841 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     9901 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     9961 caaaaataac gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca
    10021 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
    10081 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    10141 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    10201 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    10261 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    10321 aaaaaatacc gacccaggta caagtcccag gtcagctaca attcacattt tgatagtcat
    10381 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    10441 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    10501 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
    10561 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    10621 ttgattacca cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc
    10681 tggcggcgtt gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag
    10741 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    10801 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
    10861 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    10921 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg
    10981 accgcaactg tttctccgtc tcgctctaca gtgcccaaaa acccggtgat atggccttta
    11041 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
    11101 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
    11161 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    11221 atggagccag ttatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
    11281 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    11341 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gataacatga
    11401 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtacct ggtacatggc
    11461 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    11521 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    11581 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    11641 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    11701 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    11761 tcattaccac agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag
    11821 tagaacctgc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    11881 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc
    11941 agattattga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc
    12001 tacaaaatcg tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc
    12061 atcaggctga tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt
    12121 cttcgtcgtc ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt
    12181 caagctgctg gcgatgataa tcgtcccaca tgaattcgac tttctctggc tgtttcgctt
    12241 cttcagcctg cacgaccggg ttttcaagga taaaggtcat cacatcccag cagagatctt
    12301 tcacgcccag tccgctcgcc gcagagatca gataatattt atcttcccag cccagcgctt
    12361 cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg atcttattga
    12421 acactaacca acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa
    12481 taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca
    12541 acaggacgcg gcaacgttcc aggtgcttca ggaagcggat acccagacct gcgccttccg
    12601 cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc
    12661 gtaccacacc cagacttggc accagagtgg taaacggata atccgccact ttcggtttag
    12721 ccgccgatac cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga
    12781 cgtcagccag cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg
    12841 ttttctgccg tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc
    12901 agccgccctt agcaaccagc agacgctgac cgtgtttagt catatcgccc atggtttcac
    12961 cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc
    13021 gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt
    13081 caaaacgata atcgataagc gtgttcaggt tttcgtcggc ttccatccat acgtcaccac
    13141 catcaccacc gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc
    13201 tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact
    13261 tcattttatt ctccataaat cattcgcctg cgcggggttg cgataccacc gttgtatgct
    13321 tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga
    13381 caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg
    13441 ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc
    13501 tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg
    13561 tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc
    13621 caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct
    13681 gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg
    13741 tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct
    13801 cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa
    13861 cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg
    13921 gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga
    13981 acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg
    14041 gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc
    14101 caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca
    14161 cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa
    14221 tgccaatgcc tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac
    14281 gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg
    14341 ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac
    14401 ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt
    14461 ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga
    14521 tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt
    14581 ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct
    14641 cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatggcc
    14701 ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg
    14761 accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt
    14821 gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc
    14881 gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc
    14941 actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca
    15001 gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac
    15061 aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt
    15121 gccatttttt cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt
    15181 cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat
    15241 gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc
    15301 gacgtccaac tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt
    15361 cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc
    15421 accattgctg ccctgatcga gtttatccac acggcgactc tgctacacga cgacgttgtg
    15481 gatgaatcag atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc
    15541 agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac cagcctcggt
    15601 tcactcaaag tgctggaagt catgtcagaa gctgtaaacg tcatcgcaga aggtgaagtt
    15661 ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc
    15721 tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat tctggctggc
    15781 tgtacgccgg aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc
    15841 cagttgatcg acgatttact tgattacaat gccgatggcg aacagttagg taaaaatgtc
    15901 ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc
    15961 acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt
    16021 ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt
    16081 gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga caccccttgg
    16141 cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc
    16201 ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc ataaaaacac taattcagcg
    16261 ctataacacc tgaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcgttttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgcct cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggcg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttga
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc agactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg cgcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattcataa
    48841 gactattcac aagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    48901 tattttttaa tgaaaaatca catcacctac aattaaaaac acgacatccg caccataaat
    48961 agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga ttgaccgttt
    49021 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    49081 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt
    49141 cttcctgatg atgtgcggag tctggttagg gttcggtggt gtactcgatc ccgccaaaag
    49201 cagcggctac ttgatcgtcg acatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    49261 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    49321 aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc
    49381 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    49441 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    49501 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    49561 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacatactt
    49621 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    49681 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    49741 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    49801 cctgatgctc ggctcgctgt tcctcgccca cgtcgggctg atgaagtcag aactgcatct
    49861 ggtggtggtg atgttactaa gtttgacggt gacgatgttt gttgagttct tccgcaagca
    49921 taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    49981 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    50041 tggcactgtt gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    50101 gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    50161 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    50221 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    50281 tactgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat
    50341 tgccacaatc acgctatttt attaagtcat taaaaagaca aaacaggccg cctgggcctg
    50401 ttttgtatta cttcacaaca cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    50461 catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa
    50521 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg
    50581 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    50641 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    50701 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    50761 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    50821 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    50881 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca tttcacgttc
    50941 tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt
    51001 cagctctttc gcacccgggc cgctgagctc gatacccagt tgcggcagac gccacagcag
    51061 cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    51121 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc
    51181 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    51241 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    51301 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    51361 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    51421 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    51481 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    51541 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    51601 ccagtcagaa gttgtgttac ccaataagga acgactctct ttgttcgaaa atcaaacaaa
    51661 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    51721 ccattgtggt gagtggatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    51781 ttgacttaga cccttattgg cgatgtggtt tttgttttgc ctgtctttca gacggcagca
    51841 aaaagcaact tcccattttt tacgctgatt cagaatttag ccataaaaaa acccgccgaa
    51901 gcgggttttt tcgtaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    51961 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    52021 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    52081 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    52141 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    52201 tgcgggcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    52261 cgttgcccgg tttgatgaaa acgcgagctg cggaattttt gcggcgacca gtgccgtagt
    52321 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    52381 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    52441 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    52501 caatcatctc ttcaaagctc acttgtttga taccaccgat gtggccggtg tggtgatagt
    52561 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    52621 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgtaggcgac
    52681 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    52741 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    52801 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    52861 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact
    52921 ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt
    52981 tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg
    53041 catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata
    53101 aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc
    53161 atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac
    53221 atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg
    53281 ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt
    53341 cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag
    53401 cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag
    53461 atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc
    53521 acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg
    53581 ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacaa
    53641 ggctttcatc agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc
    53701 aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt
    53761 ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag
    53821 gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc
    53881 gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg
    53941 tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt
    54001 gctattgatg agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc
    54061 gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct
    54121 ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa
    54181 aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg
    54241 gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa
    54301 aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat
    54361 cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt
    54421 gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag
    54481 ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta
    54541 tcagcacatg gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc
    54601 gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc
    54661 tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt
    54721 tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat
    54781 ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt
    54841 ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaccccagc tgttgagtgc
    54901 attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc
    54961 aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct
    55021 tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga
    55081 agaattcaaa aagttttttg gtgatgattt accggagcaa cctgcacaac ccttcgaagg
    55141 tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt
    55201 gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa
    55261 actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa
    55321 attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc
    55381 ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg
    55441 acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa
    55501 ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac
    55561 tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat
    55621 ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg
    55681 catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg
    55741 tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaagc
    55801 gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc
    55861 tcgtatcgcg accaccgagc cgggcacaaa agtgaaactt ggcctgctgc gtaacggcaa
    55921 accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat
    55981 gatcgcgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa
    56041 aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa
    56101 agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa
    56161 agtgctggcg gcaaaatcgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat
    56221 ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt
    56281 aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag
    56341 ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct
    56401 tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct
    56461 gccagctata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt
    56521 ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc
    56581 atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc
    56641 atcgtcgcct tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta
    56701 accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat tccaatcaat
    56761 gcacgtcgcg taccgcatat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc
    56821 gggcagacca ttacccaggg gattattagt gccacgggtc gaattggtct gaacccgacc
    56881 gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg
    56941 ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac
    57001 gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt
    57061 atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggcat tggcggacgt
    57121 gagatcgcac cgctgcacgc acagggcggt ggtatagatc aactgcaagg gatcgtggtt
    57181 aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt
    57241 atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa
    57301 attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg
    57361 caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc
    57421 ggagtctgtg
//
LOCUS       DAQSWQ010000014        56351 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.014, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000014 DAQSWQ010000000
VERSION     DAQSWQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.014"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1334)
                     /locus_tag="R8G18_000879"
     CDS             complement(<1..1334)
                     /locus_tag="R8G18_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEE9794663.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
     gene            complement(1380..2138)
                     /locus_tag="R8G18_000880"
     CDS             complement(1380..2138)
                     /locus_tag="R8G18_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794664.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2170..2883)
                     /locus_tag="R8G18_000881"
     CDS             complement(2170..2883)
                     /locus_tag="R8G18_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HEE9794665.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3057..3749
                     /locus_tag="R8G18_000882"
     CDS             3057..3749
                     /locus_tag="R8G18_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HEE9794666.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="R8G18_000883"
     CDS             complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="R8G18_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HEE9794667.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(5589..7448)
                     /gene="gss"
                     /locus_tag="R8G18_000884"
     CDS             complement(5589..7448)
                     /gene="gss"
                     /locus_tag="R8G18_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HEE9794668.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            7653..8519
                     /gene="yghU"
                     /locus_tag="R8G18_000885"
     CDS             7653..8519
                     /gene="yghU"
                     /locus_tag="R8G18_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEE9794669.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(8642..8890)
                     /gene="hybG"
                     /locus_tag="R8G18_000886"
     CDS             complement(8642..8890)
                     /gene="hybG"
                     /locus_tag="R8G18_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HEE9794670.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(8903..9244)
                     /gene="hypA"
                     /locus_tag="R8G18_000887"
     CDS             complement(8903..9244)
                     /gene="hypA"
                     /locus_tag="R8G18_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEE9794671.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(9237..9725)
                     /gene="hybE"
                     /locus_tag="R8G18_000888"
     CDS             complement(9237..9725)
                     /gene="hybE"
                     /locus_tag="R8G18_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HEE9794672.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(9718..10212)
                     /locus_tag="R8G18_000889"
     CDS             complement(9718..10212)
                     /locus_tag="R8G18_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HEE9794673.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(10212..11915)
                     /gene="hybC"
                     /locus_tag="R8G18_000890"
     CDS             complement(10212..11915)
                     /gene="hybC"
                     /locus_tag="R8G18_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HEE9794674.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(11912..13090)
                     /gene="hybB"
                     /locus_tag="R8G18_000891"
     CDS             complement(11912..13090)
                     /gene="hybB"
                     /locus_tag="R8G18_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HEE9794675.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(13080..14066)
                     /gene="hybA"
                     /locus_tag="R8G18_000892"
     CDS             complement(13080..14066)
                     /gene="hybA"
                     /locus_tag="R8G18_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HEE9794676.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(14069..15187)
                     /gene="hybO"
                     /locus_tag="R8G18_000893"
     CDS             complement(14069..15187)
                     /gene="hybO"
                     /locus_tag="R8G18_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HEE9794677.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(15376..15663)
                     /locus_tag="R8G18_000894"
     CDS             complement(15376..15663)
                     /locus_tag="R8G18_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HEE9794678.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(15782..16669)
                     /locus_tag="R8G18_000895"
     CDS             complement(15782..16669)
                     /locus_tag="R8G18_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEE9794679.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            16826..17866
                     /gene="gpr"
                     /locus_tag="R8G18_000896"
     CDS             16826..17866
                     /gene="gpr"
                     /locus_tag="R8G18_000896"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEE9794680.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(17906..18400)
                     /locus_tag="R8G18_000897"
     CDS             complement(17906..18400)
                     /locus_tag="R8G18_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEE9794681.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            18591..19475
                     /gene="yghA"
                     /locus_tag="R8G18_000898"
     CDS             18591..19475
                     /gene="yghA"
                     /locus_tag="R8G18_000898"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HEE9794682.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(19635..20060)
                     /gene="exbD"
                     /locus_tag="R8G18_000899"
     CDS             complement(19635..20060)
                     /gene="exbD"
                     /locus_tag="R8G18_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HEE9794683.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(20067..20801)
                     /gene="exbB"
                     /locus_tag="R8G18_000900"
     CDS             complement(20067..20801)
                     /gene="exbB"
                     /locus_tag="R8G18_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEE9794684.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            21053..22240
                     /gene="metC"
                     /locus_tag="R8G18_000901"
     CDS             21053..22240
                     /gene="metC"
                     /locus_tag="R8G18_000901"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEE9794685.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            22380..23039
                     /locus_tag="R8G18_000902"
     CDS             22380..23039
                     /locus_tag="R8G18_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEE9794686.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(23079..23978)
                     /gene="yqhC"
                     /locus_tag="R8G18_000903"
     CDS             complement(23079..23978)
                     /gene="yqhC"
                     /locus_tag="R8G18_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HEE9794687.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            24172..25335
                     /gene="yqhD"
                     /locus_tag="R8G18_000904"
     CDS             24172..25335
                     /gene="yqhD"
                     /locus_tag="R8G18_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEE9794688.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            25440..26267
                     /gene="dkgA"
                     /locus_tag="R8G18_000905"
     CDS             25440..26267
                     /gene="dkgA"
                     /locus_tag="R8G18_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HEE9794689.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            26467..27393
                     /locus_tag="R8G18_000906"
     CDS             26467..27393
                     /locus_tag="R8G18_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HEE9794690.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            27444..27701
                     /gene="yqhH"
                     /locus_tag="R8G18_000907"
     CDS             27444..27701
                     /gene="yqhH"
                     /locus_tag="R8G18_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HEE9794691.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(27744..29963)
                     /locus_tag="R8G18_000908"
     CDS             complement(27744..29963)
                     /locus_tag="R8G18_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEE9794692.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(30074..31486)
                     /gene="ftsP"
                     /locus_tag="R8G18_000909"
     CDS             complement(30074..31486)
                     /gene="ftsP"
                     /locus_tag="R8G18_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HEE9794693.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(31561..32298)
                     /gene="plsC"
                     /locus_tag="R8G18_000910"
                     /gene_synonym="parF"
     CDS             complement(31561..32298)
                     /gene="plsC"
                     /locus_tag="R8G18_000910"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HEE9794694.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(32531..34789)
                     /gene="parC"
                     /locus_tag="R8G18_000911"
     CDS             complement(32531..34789)
                     /gene="parC"
                     /locus_tag="R8G18_000911"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEE9794695.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(35335..35817)
                     /locus_tag="R8G18_000912"
     CDS             complement(35335..35817)
                     /locus_tag="R8G18_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEE9794696.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(35870..36262)
                     /gene="ygiW"
                     /locus_tag="R8G18_000913"
     CDS             complement(35870..36262)
                     /gene="ygiW"
                     /locus_tag="R8G18_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HEE9794697.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            36414..37073
                     /gene="qseB"
                     /locus_tag="R8G18_000914"
     CDS             36414..37073
                     /gene="qseB"
                     /locus_tag="R8G18_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HEE9794698.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            37070..38419
                     /gene="qseC"
                     /locus_tag="R8G18_000915"
     CDS             37070..38419
                     /gene="qseC"
                     /locus_tag="R8G18_000915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HEE9794699.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            38529..39110
                     /gene="mdaB"
                     /locus_tag="R8G18_000916"
     CDS             38529..39110
                     /gene="mdaB"
                     /locus_tag="R8G18_000916"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HEE9794700.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            39141..39455
                     /locus_tag="R8G18_000917"
     CDS             39141..39455
                     /locus_tag="R8G18_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEE9794701.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(39503..41395)
                     /gene="parE"
                     /locus_tag="R8G18_000918"
     CDS             complement(39503..41395)
                     /gene="parE"
                     /locus_tag="R8G18_000918"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEE9794702.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(41424..42005)
                     /gene="yqiA"
                     /locus_tag="R8G18_000919"
     CDS             complement(41424..42005)
                     /gene="yqiA"
                     /locus_tag="R8G18_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HEE9794703.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(42005..42832)
                     /gene="cpdA"
                     /locus_tag="R8G18_000920"
     CDS             complement(42005..42832)
                     /gene="cpdA"
                     /locus_tag="R8G18_000920"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HEE9794704.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(42857..43279)
                     /locus_tag="R8G18_000921"
     CDS             complement(42857..43279)
                     /locus_tag="R8G18_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HEE9794705.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(43280..43909)
                     /gene="nudF"
                     /locus_tag="R8G18_000922"
     CDS             complement(43280..43909)
                     /gene="nudF"
                     /locus_tag="R8G18_000922"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HEE9794706.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            44114..45595
                     /gene="tolC"
                     /locus_tag="R8G18_000923"
     CDS             44114..45595
                     /gene="tolC"
                     /locus_tag="R8G18_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HEE9794707.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            45743..46414
                     /locus_tag="R8G18_000924"
     CDS             45743..46414
                     /locus_tag="R8G18_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HEE9794708.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            46420..47580
                     /locus_tag="R8G18_000925"
     CDS             46420..47580
                     /locus_tag="R8G18_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEE9794709.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(47618..48406)
                     /gene="ygiD"
                     /locus_tag="R8G18_000926"
     CDS             complement(47618..48406)
                     /gene="ygiD"
                     /locus_tag="R8G18_000926"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEE9794710.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            48549..49322
                     /gene="zupT"
                     /locus_tag="R8G18_000927"
     CDS             48549..49322
                     /gene="zupT"
                     /locus_tag="R8G18_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEE9794711.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(49380..49550)
                     /locus_tag="R8G18_000928"
     CDS             complement(49380..49550)
                     /locus_tag="R8G18_000928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794712.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(49811..50464)
                     /gene="ribB"
                     /locus_tag="R8G18_000929"
     CDS             complement(49811..50464)
                     /gene="ribB"
                     /locus_tag="R8G18_000929"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEE9794713.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(50568..50716)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            50838..51128
                     /gene="ubiK"
                     /locus_tag="R8G18_000930"
     CDS             50838..51128
                     /gene="ubiK"
                     /locus_tag="R8G18_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HEE9794714.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            51411..51962
                     /locus_tag="R8G18_000931"
     CDS             51411..51962
                     /locus_tag="R8G18_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HEE9794715.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            52022..54526
                     /locus_tag="R8G18_000932"
     CDS             52022..54526
                     /locus_tag="R8G18_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEE9794716.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            54542..55291
                     /locus_tag="R8G18_000933"
     CDS             54542..55291
                     /locus_tag="R8G18_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEE9794717.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            55293..>56351
                     /locus_tag="R8G18_000934"
     CDS             55293..>56351
                     /locus_tag="R8G18_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794718.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT        14375 a        14723 c        13826 g        13427 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcga agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt
     1441 tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag
     1501 ccaatcttcg acatgacacc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg
     1561 cgggagatgg cagtctcaat atcaatgccc aggcgaataa ttaattccgg gcgatattgc
     1621 gccatttgtt ggtataaacg ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc
     1681 gcacgttcga cgccaatccc cggtccgtcg taataaaagc ccgaaatttc agcctgcggg
     1741 aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc
     1801 gccattcttc gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg
     1861 gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca
     1921 accaacggta atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     1981 cgctcggttt gccagtgctg ttgcaatgat ttcaccagat cggtggtcag cgtggattta
     2041 ccggtgccat cgcacccgac caccgcaatt agccccggaa tgtagctggg ctgcggcgcg
     2101 gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca
     2161 ttttggtgat caggaaagcg cggcgtgaat tgcacgtagt gacgcttgca ggatttcatc
     2221 ggctggttgc cgaccatcca gttcaaggat cctggcgccg ttgaaagtca gttgcggcgt
     2281 aacggcgatt ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt
     2341 ttgttcatca atgccaagac gaatcaacaa tacgggcaaa taagatgcca tccattggta
     2401 cagcttcagc tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc
     2461 caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat
     2521 gagcagaaag ccttgctggc ttttacacaa cattttgcga aacttgtacg cccgccagca
     2581 ggaaagcaga tagataacca gagcagtaat attgccaggc ggtgttgagg gtttttcgtg
     2641 cacatgtgcc gctttacttc gcagataacg cccaaaaggt gcgccaataa cagggagctg
     2701 tgaaatccat tcgccaatta gcccggacga ttgcccgaga taaatgtgtt ctgttggcat
     2761 tcttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca
     2821 tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt
     2881 cacgaaaaac ccggcagtaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc
     2941 cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa
     3001 aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc
     3061 agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg
     3121 tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag
     3181 gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt accgttgatg ggaaaatcct
     3241 tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcct agaccagttc
     3301 cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg
     3361 cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg
     3421 gcgcttacga tggtacggtg ttcccaccta acgttgaagg tggtcgtttt gtctcatggc
     3481 tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataagcct gatctggtca
     3541 tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa acccgaccat aaacgggaat
     3601 cgctggcgag gaagattgcc ataacgccac agttaacctt tggtggtgca caactggttg
     3661 atatcgatgc caatcagcca ctggaacagg tgttggttga tgcagaaaaa gcgattacgg
     3721 attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc
     3781 gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacagccc accagaaaga
     3841 aaaattgccg ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc
     3901 tgtaacccac cgccgtccac caccatcgtc cctgctaccg cagacgagag gacgtgtgtt
     3961 gtggagacag gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt
     4021 gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc
     4081 gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac cgccatgatg
     4141 atccacacag gagcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg
     4201 ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaggt atcagagatg
     4261 cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtaactttcc
     4321 atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga
     4381 cagtgaaact ctgttacttg cgtgccatca gtcgatgctg caggcaatgg aggttccatc
     4441 gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg
     4501 gcatcgcggg tacgggtaat ttcatagccg gacgcgttca tattgacgac gaagccagca
     4561 ggggcaatcc ccaccagtac cagcatcacc aggccgatcc ctttttgtcc gtcgttcgcg
     4621 ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg caatacgcgt ccagaatggc
     4681 ggtttacgtt tgcctttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc
     4741 tttttcgtcc cgctccagta gcgtcgcagc aggaatatca ggcctcccgc aatgaccagg
     4801 ccgacgatag gggaaacaat cagcgaggag aaaattttgg tcacttcacg caggtttaac
     4861 gcatccatca ccgatgatcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca
     4921 ccaatcaagg tgtgcgaact ggaggccggt aaaccgaaga accacgttcc caggttccag
     4981 ataatcgccg ccagcagcat ggaaaagacc atcgccaggc cgtgggttga ccccatattc
     5041 agcaacaaat cggttggcaa catatggaca atggcatagg caacgctaag tccgcccaat
     5101 aacacgccaa aaaagttaaa aaatgccgcc atcaccacag caagttgtgg ttgcatggca
     5161 cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg
     5221 tagaacaaca caaatgccag agcaagcaat aacaaaagcc ctgtgtatat atcaaggcca
     5281 acaaataaat ttagcatata ttagattacg ccattttgaa tttacgaacg gacgcattat
     5341 cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt
     5401 atttcttgtg tatcagacaa taagcattca ttcaaaacct ttagaaataa tcaaaacaca
     5461 ggtattccac tggttaatat attcacatat gaaataaata aatattagaa catataaata
     5521 ttgaatattt tgattaatcc ccgtactgat tattcttcat aatcagtacg gggttgctac
     5581 aacatgtatt actttttcac cacaattaac ggttcaatat cactctcttt tttgatgacc
     5641 agtgattcat caccgcgcaa acacgtccca ccgtagttgc cgcccacggt gaaggtacat
     5701 acctgaatgt atttaccgtc cactttcggc aaacaccaca gttgctgata gatgtttttc
     5761 tgctcggcaa atttaccgct ggttttgtcc agcacctctt catgatggct gaccaggtcg
     5821 atattgctac cacagcgacc ggcaatcggt ttcactgcgt aacctgtttt caccaattca
     5881 tcattaacag tgaaatcggt atccagcagg taacgatggt gcgggaacag cgaccagagg
     5941 atcggcagaa tcgctttgtt gccggggatc accgtccaca gcggctcaaa gactagcact
     6001 tccgggcgca gcaatacgtc gataagacgc acttcgtttt gcggatgacc ggtacggatt
     6061 ggcaccgcag caaactcacg gtcgctaact tcacgaatct gatcaaacgc ggtttcccac
     6121 gcccaggttt tccacacgca gttaaccagt cgcccttccc catcaatcag ttgcccggca
     6181 gcatcccagc ccagttcatc caatccacgc aagatacgcg tttcaaagcc cgcctggtgc
     6241 agcgcctgct ccataaactg cgcgtgatag ttttcctcga tatctttgtc ctgcatgata
     6301 tggacaaacg gacgtgcacg actgtgtttc caggcaccag ccaattcgtt aatcagccct
     6361 tccgccggat tgaagccgtt gcctttatag ccctgctccg cccaacgttc gaggatcaag
     6421 cccgcttcgg tatgacagga ggcggagtcg gcgttgtact cgtaaacctt caggccacgc
     6481 tcatccatgc agaaatccat acgaccagtg atcatatggt gacggcgacg ctgccaggag
     6541 agacgcaaac gtggccagag gattttcggg atgtcgaaca gcgccagcag gttgtcatct
     6601 ttcagcacct tgtcggttgc gtgaagatac atcaggtgca gctcgttggt ggctttaatt
     6661 agctcctgct cggcactctc ggtaatggtg tagtaatgat aaggatcctg attgatcacc
     6721 tgaccgttgg cctgcacata ggcgttttgc agcggatctt tttcatccag ccatttaccg
     6781 tcaaactggc ctttgttttc caggcgcgct ccgctgattt tcagcagctc gcctgcaatt
     6841 tccggctgcg gtaagctgta ttcagtatct tccgtctgga tcatccagcc cagaatggtg
     6901 gtgtcatcaa aagtgtcttt cagggtatag cagccgtttt cgaccaccat ctccagctca
     6961 cgcgtccact gttgcccttg cggcaacggg gaatgaatca cgttctgttc cgcaatacgg
     7021 actttgttgc catgcaattg ggtaatgatg gcgacatggc cagtgtcttt aaattcaccg
     7081 cctttatccc agataagaag cgcacccgcg accggcgcac gcggcgagcc gttaggaaat
     7141 gcctgcaatg gcaggatgtt gtcattaacc acttctcgca ggaagcgcag cgagaaaatc
     7201 tcccacgcca tacccacgtc agtaaagacc acaccgtaat tcagaaagag aaaacggcga
     7261 gcaaattcaa cgcattgcca cttgtggccc atatattcgt cgtcgatata gctacggaat
     7321 acggcgtcat cttcgtattc ctgcggatcg agagaactgt aatctgaaga gtagattgct
     7381 accccacctg gggcgtagcc caataatgtc ccgaacgggg catcctggct ggtcgttcct
     7441 ttgctcatca ctttaccttt aacaatacaa cctaagcagt tggcgtaatt gttgtgctct
     7501 gattacctgc tcagcgacat taaccggaca gaggtcagac taatcataca cctcatcgca
     7561 gcggctggcg caaagggtta aaaaattcac attctgtaca gcaagtgacc tgctacactg
     7621 cttcaacact accactcaga aggcaactca ctatgacaga caatacttat cagcccgcga
     7681 aagtctggac gtgggataaa tccgctggcg gcgcgttcgc caatatcaat cgcccggttt
     7741 ctggtccgac gcatgaaaaa acgctgcccg ttggcaaaca cccattgcaa ctttattcgc
     7801 tgggaacgcc gaacggtcag aaagtaacga ttatgcttga ggagctgctg gcgctgggcg
     7861 ttactggtgc agagtacgac gcctggctga ttcgtattgg cgatggcgat caattctcca
     7921 gcggctttgt cgaagtgaac ccaaactcga agatcccggc gctgcgcgat catacgcata
     7981 atccgccgat ccgcgtgttt gaatctggtt cgatcctgct ttatctggcg gagaaatttg
     8041 gctacttcct gccgcaggat ttggcaaagc gtactgaaac gatgaactgg ctgttctggt
     8101 tacagggcgc ggcaccgttc ctcggcggtg gttttggtca cttttaccat tacgcgccga
     8161 tgaaaattga gtacgccatc aaccgcttta ccatggaagc caaacgtctg ctcgacgtgc
     8221 tggataagca actggcgcag cataagtttg ttgcgggcga tgagtacacc attgcggata
     8281 tggcgatttg gccgtggttt ggcaatgtgg tgttaggcgg cgtgtatgat gccgcagagt
     8341 ttcttgatgc gggcagttat aagcatgtac aacgctgggc gaaagaagta ggcgaacgtc
     8401 cggcggtgaa acgtgggcgt attgttaacc gcaccaacgg accgctgaat gagcagttgc
     8461 atgagcgcca tgacgccagt gatttcgaga cgaatacgga agataagcgt caggggtaag
     8521 ggttggtgtt cgtcgcagca agccatccag gccggataag gcgttcacgc cgcatccggc
     8581 aatcgtgcac aatgcctgat gcgacgctgc cgcgtcttat caggcctacg aaagctaatc
     8641 atcacgcgct ggtaatgtcg taatccattt ggcgcagtgc gtctaatgtg gctttggctt
     8701 catcttcgtc gatgatgctc atggcaaatc cgacgtgtac cagcacccac tggcccagta
     8761 gatcggcagg gttaccttca caaatcaggg cgatattcac gtcgcgcttg ataccacata
     8821 cttcaacctg cgcaagctgg tgaatatctt caccgacagc cagcacctgg cctggaacgc
     8881 caatacacat tattaactcc ggttattcaa cttcaatact tttgacgatc agcgaatcgc
     8941 cggtatcgac acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa
     9001 tctccaccac ctggctgcaa tcccagcacc aagcctgggc gggtttatag acgatatgta
     9061 aatcgcaccc ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc
     9121 tctcctcaac gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat
     9181 cgtgctgctc cgcctgccgt tggataattt caacggcgct ctggcaaaga gacaactcat
     9241 gcattttcgc cactcctgcg accaaacagc agggcgcgac gccctgcgtg tggtacatcc
     9301 ggattcgtga ctggcagcga aaggatcatt cgtgcgcagt catccgtcag gcgttggccc
     9361 tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg aacaggagag atattgcgaa
     9421 acaccgtcca gttcgccaac ggtaaaggtc atagtgccat acggcagttg cagaccaatt
     9481 ttttcactca ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc
     9541 agcatccacg gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa
     9601 acatacaccg gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt
     9661 tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca
     9721 gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac
     9781 ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc
     9841 cagcgattcc gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg
     9901 cagggccgac aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa
     9961 cgccggaact tcttcatccc gcaggatcat catcgttccc ggcgtgttct ttttcgacac
    10021 aatggcatcc gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc
    10081 cgccgtgccg ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc
    10141 ttcgacaatc cgcacaccga tggcttcatc ggtcagcaaa atattgccga cccctaagac
    10201 taaaatacgc attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg
    10261 tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt
    10321 ttattcggat cagcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca
    10381 ttgaagttac gcggaccaga gttccaggtt gatggaacaa ctgcctggta gttactgata
    10441 ataccgtctt taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca
    10501 acacctttga actcacccgt tgccggaatg ttcggtttca caaaggtggt gtgatcgcct
    10561 ttgccgatat tggtgatcag tgcactgtat tgattttgca ggatatcctg caattcgcag
    10621 cagtgaacgg tacgaccaat aatacggccc agcgtggagt gcagctgcgc cacttccagc
    10681 gtgttgccag tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat
    10741 tcgcgacctg ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg
    10801 ccgtagaaag tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa
    10861 gccggaatgg tggtaccttc ccacggtgcc tgcggcgctt cgtctttata ccaggagtgc
    10921 ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga
    10981 tacgaggaca gatccgcatt ctcaatgtag ccgcccggga acaggaagct gccgttttta
    11041 ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc
    11101 cattccgggt agaacgcggc aataactgcg gtatcaacct tataaacctg ttcaacaaag
    11161 tcgctcagct tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt cagcacgccc
    11221 aaaccgtcga ggttgatcgg gttcgcgaca ccacctaccg ccaggttctg aatgtgcggc
    11281 gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc
    11341 aggtagtgcg ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc
    11401 ccgtagccat tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc
    11461 tgaactttag tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt
    11521 tcggaggctt tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc
    11581 gaaagctgat agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta
    11641 cggatgtatt gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg
    11701 gaagacagcg cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca
    11761 tcgcgcggat cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc
    11821 catgctttcg aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata
    11881 cgggttaccg gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat
    11941 gatcgttttg ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc
    12001 aaatctcaat agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg
    12061 cgtaaccgcc gcccgggttg aatgccacca gcgaataggt cagacgccag gttgcacaac
    12121 ctaacagtgc gctcagtgct gacaggaaca gcatgcggga atcattacgc agcttcgcca
    12181 cacgcagaac gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt
    12241 agaagtcacc ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca
    12301 gcacgatgaa aatcgccagc aacacactga tggtgttggt cagcttgacg aacagactct
    12361 tttcatccgg accgttgcca cgcagacccg cctgcaccag cgaaccttca aagatgacaa
    12421 tcgagaagcc catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct
    12481 gccacaacgg atgcaccttg tagcccgccg agatcatcag tgaccccatt gaagactggt
    12541 gcatggtcgg caacagcgcg ccaagtgcaa tgatgaagaa cataaccttg ttcagacgct
    12601 tgagagaaac cttccagccc agacgttcaa acagtgccgg agcaaactcc agtgccatca
    12661 cgccgatata gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt
    12721 gacccggaat gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca
    12781 agccacccag tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt
    12841 attgcccacg gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc
    12901 cggtgccaat cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt
    12961 cagagacaga gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga
    13021 ttaacggtcc aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat
    13081 catgactcat gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc
    13141 acggttaagc ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga
    13201 atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca
    13261 taaggcacac ccgtcagtac cagaacctga gtaccgccgc cctctttctc gccgtacaga
    13321 tgcggataat atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc
    13381 ggatagtggt attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc
    13441 tcttcacgcg taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga
    13501 ccgcctttat cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca
    13561 ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa
    13621 cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc
    13681 agtgcagaga ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc
    13741 ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac
    13801 acctgaatga tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc
    13861 ccttgcgggt tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag
    13921 cctacgcaca aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt
    13981 tcagcagccg catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct
    14041 gctttaataa aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct
    14101 tttgctgacg acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca
    14161 caccgccgag caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat
    14221 ccggtttctg tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat
    14281 ggaagccgat accttcttcg ttacagccat agcatgggtg accaatcgct accggccaca
    14341 cgccgccaac atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac
    14401 agccgaggtg gtacaggcac cagccttcac ggtggccttc atcaccgaac tctttggcaa
    14461 aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg
    14521 cgaaggtcgg acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg
    14581 caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc cggaatattg ataacggttt
    14641 tgcctggcag aacttcttgc aggctgactg cgccagttgg gttaactcca gctgcggcaa
    14701 caccgcccca cgcagagcag gaaccgatag cgataatcgc tgctgcgcct tctgccgctt
    14761 tgcggatgtg atccacaatc ggctcaccag caaccatgca ataaataccg ttatctttta
    14821 atgggatgga accatccaca accaacacat actgcccttt gtacttctca agtgcgttat
    14881 gtttgttctc ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tactccagag
    14941 agatagtctc cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg
    15001 tacaaccggt gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa
    15061 ccgattcggc catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg
    15121 cacaaagctt catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc
    15181 cagtcattta tagttattcc gttgcgaaga cctggcatat attttgcctc aatcgcaaaa
    15241 tcaataatgc gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat
    15301 actgcggcgg ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat
    15361 aatacgtatg tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga
    15421 acaggaatta ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg
    15481 cgtcagaata cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatccg
    15541 gtgtgagtag cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac
    15601 cgcactgtcg gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt
    15661 catcgttcac ctcattcgct atatatcgcc atatatagcg atattttagg caaagaacga
    15721 cttttgcgca cgctattttt tcatgcttgt ttaagacgta atccagtaag agataaacct
    15781 atcaggagag atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag
    15841 atttgtcata acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat
    15901 acgcctcata aaccttatta ttggctttca acgccgcctc gtaagcaggc cagccctcgt
    15961 tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat
    16021 cggcagtggg tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga
    16081 cagccgccgc gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttgcctg
    16141 ccgcttgtgg atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg
    16201 ttggatcaac ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa
    16261 cggaacttaa gccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca
    16321 catcttcgat atacggattc agtccacgat tctcatgcac caccaccacg gctggcgttt
    16381 tgccgctcat ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg
    16441 aaggatacat gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca
    16501 gcgcataatt tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat
    16561 acttcgccgc aaggttgagg aactcacgtt tcgaaatttt cccgtgagcg taatagtcgt
    16621 agtaatccaa caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcactcc
    16681 tcagttggtg tttttttaag caaagcataa gcacgtgttt ttgcccagtt tttcgtcact
    16741 ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga
    16801 aattgaacgg tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca
    16861 gatgcaatac cgctattgcg gaaaaagcgg tttacgcctg cccgcgttat cgctcggttt
    16921 atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcgatcc tgcgtaaagc
    16981 gtttgatttg ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag
    17041 cgcagaagag aactttggtc gcctgctgcg ggaggatttt gccgcttatc gcgatgaact
    17101 gattatctct accaaggctg gctacgatat gtggcccggc ccttacggct ctggcggttc
    17161 acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt
    17221 cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc
    17281 gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc
    17341 agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca
    17401 tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct
    17461 gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg
    17521 aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg
    17581 tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga
    17641 aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga
    17701 tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt
    17761 gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat
    17821 cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg
    17881 gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc
    17941 ccatcacaaa tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt
    18001 tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca
    18061 caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc agccagttca
    18121 gcttctcttt gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca
    18181 tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga
    18241 tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga
    18301 acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc
    18361 agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta
    18421 aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac
    18481 gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc
    18541 atcgagagcg tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt
    18601 taaaagaccc gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc
    18661 caggcatcca ggcgaagatg acaccggtac cggattgcgg cgagaaaacc tatgttggta
    18721 gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg
    18781 ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg
    18841 aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc
    18901 tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca
    18961 aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg
    19021 atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc
    19081 tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt atcatcacca
    19141 cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg
    19201 cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg
    19261 tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc
    19321 aggataagat cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg
    19381 aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag
    19441 tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa
    19501 ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta tccggcctac
    19561 aaacgcacga agattcaaca tgttgcaata gattttgtag gcctgatgag cgtagcgcat
    19621 caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc tatcttcagg
    19681 taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc gacggttttg
    19741 tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt caacgccgta
    19801 atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc tgccttcacc
    19861 gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc aggcaagttc
    19921 accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag cagaaccaac
    19981 atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc gtcgaggttt
    20041 tcgttaagat gcattgccat cggacattat cctgcgcgta atttttgtgc gacacgaacc
    20101 ggatgcgcag cggcgctggc ttccagatcc aggtcacggc tttgcagcaa caatacctgc
    20161 gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa tacgttatag
    20221 ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag agcttctgcg
    20281 atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc aataaagctg
    20341 ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc gccgatggtt
    20401 gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacccg acgctccaga
    20461 cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga cagttccagc
    20521 tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa atcagcggcg
    20581 atatcgttgg cctggtttaa ggaacgcgct tcagccagca gttgctgctc gcgcttaagg
    20641 cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca agtgactacg
    20701 gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgtg ctgatacata
    20761 ccccagacgg aaaggtccgt ctgcatcaaa ttattaccca ctgtgtatct ccaggacgca
    20821 agtcacaaaa tctgcgcata ataatatcaa aatgacgtcg aattgatagt cgttctcatt
    20881 actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca gtaaaaaagt
    20941 caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg atgcgcccgc
    21001 gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg agatggcgga
    21061 caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca ctctcggcgc
    21121 ggtaaatagc gtgattcagc gcgcttcttc gctggtcttt gacagtgtgg aagccaaaaa
    21181 gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg gaacgttaac
    21241 ccatttctcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct gcgtgctatt
    21301 tccctgcggg gcggcagcgg ttgctaattc cattcttgct tttgtcgaac agggcgatca
    21361 tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca aaatcctcag
    21421 caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata tcgttaagca
    21481 tctgcagcca aacactaaaa tcgtgtttct ggaatcgcca ggctccatca ccatggaagt
    21541 ccacgacgtt ccggcgattg ttgccgccgt acgcagtgtg gtgccggatg ccatcattat
    21601 gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg gcatcgatgt
    21661 ttctattcaa gccgccacca aatatctggt tgggcattca gatgcgatga ttggcactgc
    21721 cgtgtgcaat gcccgttgct gggagcagct acgggaaaac gcctatctga tgggccagat
    21781 ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag gtgtgcgttt
    21841 gcgtcaacat catgaaagca gtctgaaagt ggctgaatgg ctggcagaac atccgcaagt
    21901 agcgcgagtt aaccaccctg ctctgcctgg cagtaaaggc cacgaattct ggaaacgaga
    21961 ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca gtaatgaaga
    22021 gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt ggggcgggta
    22081 tgaatcgttg atcctggcga atcaaccaga acatatcgcc gccattcgcc cacaaggcga
    22141 gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg tcgacgatct
    22201 gattgccgat ctggacgccg gttttgcgcg aattgtataa cattgccact tttggacaat
    22261 tttgcagaca tttaattgtg aaaagtctta aattgttgcg tccgggatca aggcgtcccg
    22321 gacgaatcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca ggaaagtcca
    22381 tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc gcgctggcgg
    22441 atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt ttagaaaacg
    22501 gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca ggcgcattga
    22561 ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc gcagcaagtc
    22621 tgggctgctg gctaagttat attcaggggc gctggttagg gaataccaaa acggtgaaag
    22681 gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg tttgaccgcc
    22741 acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg ctgctgccaa
    22801 ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac tggttaagtg
    22861 gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg attccgttcg
    22921 ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt gccttgttaa
    22981 ccgctggctt gttaggcacg ctgtttgtag tgattaaaaa aaaatactgt aacgcctgac
    23041 gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg catcgcccgc
    23101 attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc acggctaaat
    23161 tgcaatgcgc tttcatagtc gacgcgcatc gctgctgcgc tggccttcat gccgtcatgg
    23221 atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa cggcgaggtg
    23281 ctggtgacag acttaaaatt atgatggaac gccgatacgc tcatgttggc ttctgccgcc
    23341 agttgctcga cgctcaggtt ttcggtgtat ttattctcaa tccgtttcag cacgcggcta
    23401 atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc gcaaggtccg
    23461 gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg cgcatccagt
    23521 ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc tgataacgtg
    23581 gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc gccaatgtcc
    23641 atcaacagtt cctgtaactg caaaatatcg acattgagac gcaatcctgc cagcggcacc
    23701 tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag caggtattca
    23761 ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc ggaaaagaga
    23821 attatgatgc caggctcgta catcaccggt gtacgtgcga aaggcgtctc gccatacaac
    23881 aaacgcacat cgggcaacag ttctgacaaa ctattttctt tatttttcag tttattaact
    23941 ttatccgcca gcaagcggca aatctcttca cgtttcatat cgcgtaattt cttaggaata
    24001 atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg gagaaatagg
    24061 caagacattg gcagaaatga gcattgagag ccagggcgct ggcgatcaca atgaaaaaca
    24121 tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt aatgaacaac
    24181 tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc tggtttacgc
    24241 gaacaaattc ctcacgatgc tcgcgtattg attacctacg gcggcggcag cgtgaaaaaa
    24301 atcggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct ggaatttggc
    24361 ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact ggttcgcgaa
    24421 cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg caccaaattt
    24481 atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct gcaaacgggc
    24541 ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc agcaaccggt
    24601 tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa gcaggcgttc
    24661 cattctgccc atgttcagcc ggtatttgcc gtgctcgatc cggtttatac ctacaccctg
    24721 ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt ggaacagtat
    24781 gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat tttgctgacg
    24841 ctaatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt gcgcgccaac
    24901 gtcatgtggg cggcgactca ggcgctgaac ggtttgattg gcgctggcgt accgcaggac
    24961 tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga tcacgcgcaa
    25021 acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa gcgcgctaag
    25081 ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgacga tgagcgtatt
    25141 gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc gacccacctc
    25201 tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact ggaagagcac
    25261 ggcatgaccc aactgggcga aaatcatgac attacgttgg atgtcagccg ccgtatatac
    25321 gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt tcgtccagac
    25381 ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag gaggaacgta
    25441 tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag ctgggactgg
    25501 gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg ttagaagtgg
    25561 gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc ggcaaagccc
    25621 tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg tggaacgacg
    25681 accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag cttgattata
    25741 tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc gaagcatgga
    25801 aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg tgcaacttcc
    25861 agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg ataaaccaga
    25921 tcgaacttca tccgctgatg caacaacgcc agctacacgc ctggaacgcg acacacaaaa
    25981 tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc gatcagaaag
    26041 tcattcgcga tctggcagat aaatacggca aagccccggc gcagattgtt atccgctggc
    26101 atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt attgccgaaa
    26161 actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcgaaatt gcaaaactcg
    26221 atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacat gcaaattctc
    26281 ccggtgacgg taatgttccg ctaccggact tttcagaaat catttattcc cctcgcgtcc
    26341 cgcccgttgt tactcttcct tgttcaggaa tgccaaatat aaggacatca tcatgcagag
    26401 ccggaagctc ttaaaagaac aactcatcta tatccgggat aaacgcaacg gagaggtgaa
    26461 aaacagatga aaataatact tctgttttta gcagccctgg caagttttac cgtacacgcg
    26521 cagcctcctt cacagaccgt agaacaaaca gtccggcaga tttatcagaa ctataaatca
    26581 gatgccagta caccttattt cggtgaaacc ggagagcggg cgataacttc agcgcgtatt
    26641 caacaggcgc ttaccctgaa cgacaatctt acgctgccgg gcaatattgg ctggctggat
    26701 tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgctagaaag cgttgcgata
    26761 acccaaactg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt taaagatgat
    26821 aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg ggtcattgac
    26881 gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa tgaaaaaacg
    26941 ctggccgctt tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc cgaactcttt
    27001 gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc ttaccgccag
    27061 gcggttaatg ccttctataa aaccaccttc aagacggcca ataatcccga tgaagatatg
    27121 caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga gtcgctattt
    27181 tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg cattcatgtt
    27241 cgttttacgc tgaccaatgg caacaacgaa gagcaagaac tggttttaca gcggcgcgaa
    27301 ggcaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact taagcagatt
    27361 gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa ttagccggaa
    27421 caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct gttgttctgg
    27481 caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcaactg gcgagcaatg
    27541 tacaaacatt aaatgccaaa atcgcccggc ttgagcagga tataaaagca ctacgcccac
    27601 aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat gctcaggact
    27661 attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat ccccggcagc
    27721 atgtcagttg ccggggattt tttttaacgt ccaaccgccg ctttagggcg tttcttcgca
    27781 ccagcattca ccggacgaga ttgcgtagac gacgcttttt ttgccgtagc aggcgtctga
    27841 cgctgggtcg ccatcggcgt atgtttagtc aacgccggac gggtattgcg gttctggcga
    27901 cgagcttcac gcatctcttc aatggttggc gcaggcacta agcaatcgcg acggctgcca
    27961 atcagatgct ttttgcccat cgcttccagc gccgggcgga ttaacggcca gtttgccgga
    28021 tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgtt tgtcgccctt cggtacgaag
    28081 acgtcttcgc tcttataacc aatcttcgcc agcggttttt tcccggtgta atacatggtg
    28141 gttgagttcg ccagcggcga tgggtagaag ttctgtacct ggtcgagacg gaaacgatgc
    28201 tttttcagcc acagcgccaa attcaccata tcttcatcac gcgtaccggg gtgcgcagag
    28261 atgaaatacg ggatcagata ctgctcttta cctgcctgtt tcgagtaggt atcgaacagc
    28321 tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgacaa cggcccttct
    28381 tcggtatgtt ccggagcaat cttcagataa ccgccgacgt gatgggtcgc caactctttg
    28441 atatagcgcg gatcttccac ggcgatgtca taacgtacgc ctgaggcgat gaggattttt
    28501 ttaatgcctt tcagatcacg cgcacggcga tagaggttga tcgtcggttc gtggttagtg
    28561 tccatgtgcg gacaaatatc cggataaacg cacgacaaac ggcgacaagt ttgttcagcg
    28621 cgtggcgatt tgcagcgcaa catatacatg ttggcagttg gcccaccgag atcggaaatc
    28681 acgcccgtaa aacctggaac ggtgtcgcgg atcgcttcga tctcattaat gatcgaatct
    28741 tccgaacggc tctgaataat gcgcccttcg tgctcggtaa tagaacagaa agaacagccg
    28801 ccaaagcagc cgcgcataat gttgaccgaa aaacggatca tttcgtaagc cggaatacgg
    28861 gcattgccat aggccggatg tggcacgcgc ttgtacggca gcgcaaaaac gctgtccatc
    28921 tcttcggtag aaagcgggat agcaggcggg ttgatccaca catagcggtc accgtgtttt
    28981 tgcatcaatg cgcggacgca gcctgggttg gtttcgtggt gcagaatacg cgaagcatgg
    29041 gcgtacagca ctttatcgcc cttcactttc tcgaaagaag gcagcaacac gtaggttttt
    29101 tcccacggtt tcgggcgcgg tggctgcacg gttacggctt tggcttcctg ctttttcggt
    29161 gccaccggtt tgttatccgc gcacggcaaa tcttcaccat acggatgcgg gattgggtcg
    29221 atttttccag gggtatcaag acgggtggaa tccacgccgc tccagccagg cagcgcctct
    29281 ttcacgataa tcgcggtatt acgcacatcg cggatttcac taatcggctc gcccatcgcc
    29341 agacggtgcg ccacctccac cagcggacgc tcaccgttac caaacatcag catgtcggct
    29401 ttcgaatcca ccagcacgga acggcgcacg gtatcggacc agtaatcata atgtgcggta
    29461 cggcgcagac tagcctcaat accgccgagg atcaccggta catctttcca cgcctcttta
    29521 caacgctggg tataaaccag tgtggcgcga tccgggcgct tacccgcgac gttatccggc
    29581 gtgtaggcat cgtcatgacg taaacggcga tcggcggtat aacggttgat catcgaatcc
    29641 atgttgccag cagtaacacc gaaaaacaga ttcggtttac ccagacgcat aaagtcgtct
    29701 ttgctgctcc agtccggctg ggcgatgatc ccgacgcgaa agccctgtgc ttccagcata
    29761 cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc agtaaccaaa
    29821 atgatgtcgc agctatccca gccaagttga tccatctctt cacgagacat cggcaaaaac
    29881 ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc gcgatccggt
    29941 tggatcaggg agatagagct cattttgctt ccagaaatga taaaaaaata atcaaaggcc
    30001 ggggattata agccggaacg aaagagaaat cgaaaagtat tccatactcg ccctcctcgg
    30061 gcgagtatga agattacggt accggattga ctaacagttg cccaatcgag ccacggtccg
    30121 ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa ggctgaccga
    30181 aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag cctctgtctt
    30241 ccgggaacgg catcgcacca ttcacgttac ggatctggaa catcacgcct tcaatatgga
    30301 acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca cgttccttgc tgcgcggtga
    30361 catcaatacg gttgacgtcc cacagctgtc cattaatacc cgggtcatca cccagactga
    30421 tatcgcgact gcgaatcggc gaaccggcca tgatttcagt tggcagcaag cgcatcggaa
    30481 gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc agggtagaaa
    30541 ccagaatact ggatggctca aagaagccgc gaatacgatc aacaatgctc gccgcttcgc
    30601 cacaggtgat cgacacttca tcgccgttgc tcatatccac cagaatctcg cggcgctcgc
    30661 ccggtgccag cgaaagttgc ttcaccgata caggagcagg caggaatccc tgatcgccag
    30721 aaatcacatg taacgggcga ccatcgctca tctgtagttg atagcgacga gagttcgacg
    30781 cgttcagcag tcgcaagcgc acccagccac gcgagacttc aacgtacggg ctttgtacac
    30841 cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgttg tattctggcg
    30901 taccaaagtt atccagccgt ttatcctgga taatgaccgg aaaatcatcc acatcataat
    30961 ggttggggat aggcagcgac ttgctgactt catcttccac cagccacatt ccggcaaggc
    31021 cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac agagtagctg
    31081 cgttctggcg aataggcagt acgggtgccc agtcagcgtt tggcgacatc atccgtgccg
    31141 gaccgcccat cagcgggcca ggtacctgta gcccggccac cgtcattgag acattttctg
    31201 tcaggcggtt gctgtaaata agcttaacat cgtcgccctt ccagacgcgg atagtcggcc
    31261 ccaggtaacg accattgatt ccccagaccg acgcgcgtgt ccctggcgta aatgaccagt
    31321 gcgcacgttg tacagtcata aacagcggtt gcccacggcg agattcaagt agcggcggaa
    31381 cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg cctgcacaaa
    31441 gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt gctccatgta
    31501 aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca gattgccgac
    31561 ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg cgattttttg
    31621 ttccattatc gaacgacaat gggcagccag ctcacgaacc tgatctttgc catactgact
    31681 gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgcagtc gattaagatt
    31741 aatcttattc gaagttgtag agacgcacac gggaataatc gggacgcccg ccgcaattgc
    31801 cgcgtgaaat gctccagtct tgaacggtag caggccgcga ccacggctgc gggttccttc
    31861 cgggaacatc cagatggaaa tacggcgttt tttgaaatga ttcacgactt ccgcaatggt
    31921 gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta accagtacaa
    31981 ctgcccgaag aaggggatcc acagcaagct ctttttacct accgtcaccg tcggcggttg
    32041 cacaatgttc gatgctgtca ccatgtcata gttgttctgg tggttagcga tatagatagc
    32101 attgccgtag ctttcagcat ctgcaggttt gcggcactca actttcaggc cgaacagcgg
    32161 cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac gcgggctgaa
    32221 aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg taataataag
    32281 acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt gtacgtcacc
    32341 tgaaatcagg ttagggcatt gttaaaagcc gctctctgga aaagcgtatt gttaatcgca
    32401 acgcatgaat aaacaacggt ttcaagggaa tttttatcgt ttccgggggt tgaatacgct
    32461 gccggatgac gacttaacgt ttcatccggc gttccttgca agcgggagga aacaggccct
    32521 ccccggcata ttactcttcg ctatcaccgt tgctggcacg gcgaggagag tcgatctcaa
    32581 cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg gcgtccacgt tcaccagtga
    32641 ctttctgtaa ctcttccggg cgcagtttaa ttttgcgttt cccaacatga atggtcagcg
    32701 tgctttgcgg cggcagaacg tacaattgcg ccagaccatc ttctccacgc gcggcttctg
    32761 ccgatggaat gttgataatc ttgttgcctt tgcccttcga cagctgcggc agatcgctta
    32821 ccgggaacat caacatacgg cctgcctgag tgattgccag cagcatatcg gaagcatctt
    32881 caatcaccac cggcggcata acatgggcat tttccggtaa ggtgatcaaa gccttacctg
    32941 cacggttacg cgccaccaga tcgttaaagg tgcagacgaa accgtaaccc gcatcggaag
    33001 ccatcagcag tttctgatcg tcgctttcca tcagcatatg gtcaacggtc gccccaggcg
    33061 gcaacgttaa tttgccggtg agtggttcgc cctgaccacg cgccgacggc agcgtaatcg
    33121 ggtcgatggc atagctacga ccggtggaat caacaaacac taccgattgg ttgctcttac
    33181 ctttcaccgc cgctttgaag ctatcacccg ctttataatt caggcccggc gcgtcgatat
    33241 catggccttt agcgctgcgt acccagccca tctgcgacag cacaatggtg acaggttcag
    33301 acggcagcat gtcgtgctcg ctcatcgctt tcgcttcttc gcgttcctgc aacggcgaac
    33361 gacgatcgtc accgtaggct tgcgcgtctg cctgcagttc tttcttcagc aggttattca
    33421 ttttacgctc ggaagccaaa atgccctgca actggtcgcg ctctttttcc agctcactct
    33481 gctcaccgcg aatcttcatc tcttccagtt tggcaagatg acgcagtttc agttcgagga
    33541 tcgcttccgc ctgggtttcc gtaaggccaa accgcgacat cagcgccggt ttcggttcat
    33601 cttcgttacg aatgatctca atcacttcgt cgatgttgag aaacgccacc agcaaacctt
    33661 ccaggatatg caggcgcttg aggactttct ccagacgata gttcagtcgg cggcgcacgg
    33721 tatcgcgacg gaacaccagc cattcggaga ggatttccag caggtttttc accgccggac
    33781 gaccatccag accgatcata ttgaggttaa tgcgatagct cttttccaga tcggtggtag
    33841 cgaagaggtg gttcatcacc tgatccatat ccacgcggtt ggaacgcggc acaatcacca
    33901 gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc gtcaaccatc ggcagctttt
    33961 tgttgcgcat ttgcgcagca atttgctcca gtacacgcgc acctgaaacc tgatgcggta
    34021 atgcgctgat aaccaccgcg ccatcttctt tcttccacac cgcgcgcata cgcactgaac
    34081 cacgtccgtt ctcgtagatt ttacggatct cggcgcgcga agtgataatt tccgcttcag
    34141 tcggataatc cggcccctgc acgatatcca gcagctgatc gagcgtggtt ttcggctggt
    34201 cgattaatgc gattgccgcc tgagctactt cacgcaggtt atgcggtgga atatcggtcg
    34261 ccatgccgac ggcaataccg gtggtgccgt taagcaaaat gtttggcaga cgagcaggca
    34321 gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac ccagtcagcc gtcccctgcc
    34381 ccagctcgct caatagcagc tcggaatatt tcgacaaccg ggattcggtg taacgcattg
    34441 ccgcgaacga tttcggatcg tccggcgcgc cccagttccc ctgaccatca accagcggat
    34501 aacggtaaga gaacggctgc gccatcagga ccatcgcttc ataacaggcg ctatcgccgt
    34561 gcggatggta tttacccagt acgtcaccga cggtacgggc cgatttttta aatttggcgc
    34621 tggcattcag gcccagttca gacatcgcat acacaatgcg gcgctgaaca ggtttcagac
    34681 catcaccaat aaacggcaac gcacggtcca tgatcacgta catggagtag tttaagtagg
    34741 cgttttccgt aaattcatgt agcgcaaggc gctctgccat atcgctcatt aattctgatt
    34801 cctcaactga ttcgccagcc ttcaaagggg caatattgcc ggagatacta cattatctgc
    34861 cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg gacgtgctcc gacggcgata
    34921 aaggcatcaa tgtgccttga tatacaactc tttgctgtag acttgatcgg catagagggc
    34981 agcacaaggt tgcgcccatg ccacaccact cggcatgggg gtccgctccg ggcgattcga
    35041 gaagttgagg ttatgcgagt ccagggaagc ctaaagcatc aaagtagtcg actgtaagta
    35101 tatggttcag cccagtttag ctgtgtctcc accagtgccc cgcgcctcct gccataagtg
    35161 cggcttgctc atgggtaccc ccgagacggc ttacggcgca ccggataata aatttcgcgt
    35221 atacatggta ttccttaccg gagaaattat aagcccaaca acaatacgcc tgtggcattg
    35281 ttcgctcaaa cttatcgcga gtgatttggc tcacactccg gtaaagattc cccatcacgc
    35341 taacggcaca taaacatccg tttccagtcg ctgctccgtc accccttcgg caagattggt
    35401 gtagtgaaac agaatcggtg ctttacgcat tttctcgccg cttgcaggca gccagtggcg
    35461 aataatgccc catatcgtgt gtgaaatatc gtccagctcg ccaacgtggc gggccacggc
    35521 ataacgtcca ccggtaagtt caccattgct cacaccataa cgattatcgg gaatcggttc
    35581 gctaacgctg ccgcagatat caaagcgaaa cgcttccggt gcggtggtgg cagggtcgtc
    35641 ccaggcgacg ccaaaagtct ggctttggtt aacaggagaa agccccgtct ctttacgcca
    35701 catgataaat ttcgccacac tgtagttgag caactcaggg ctacagcggt gcggcaacat
    35761 cgccacaggg attgatggga aatcgataat gtttacatcc agtgtcaggt ttgtcatttt
    35821 gcctccctgc gaaattattc ccgggaacgc gtcccgggag cggtaacaat tacggattta
    35881 ctttgcggat ctgtttgacg tcaatttcaa cagaattcca gtctttatcg acttcaccct
    35941 gaatctcaac cgtatctttc ggcgtcaccg tcacaccgtt ccagcgtttg tggtcgatat
    36001 caacattgat agtaccgctg gcatctttaa acacgtagag atcgtcagag atgcgttcaa
    36061 cgatattgcc gcgcagggtt acccaggtgt cgtcacgcag ggattttgcg ctttctacag
    36121 tcgttacgct gccgttcggc ccctggaatc ctccggcctg actttgcgtt gccgatgggc
    36181 cagaaaaacc gccctgctct gctgccatca ccggtgcact gcacagggcc attactgcga
    36241 ttactgctgc gaattttttc atgtttatta ctccctttaa tgtctgtttc cgagcattta
    36301 acaagatagt ccttaacaac ttcttaaggg aaaaaaataa aatttaatgc tgtacagagc
    36361 gcgttacaac acggtttact ggcagcaaat acggttatcg cagggatgaa aaaatgcgaa
    36421 ttttactgat agaagatgac atgctgattg gcgacggcat caaaactggc cttagtaaaa
    36481 tgggttttag tgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg ctttatagcg
    36541 caccttatga tgcggtgatc ctggatttaa ccttaccagg catggatggt cgcgatattt
    36601 tgcgcgaatg gcgagagaaa ggtcagcgtg agccggtact gatcctgacc gcgcgtgatg
    36661 cgttagcgga acgtgtagaa gggctgcgtc tgggagctga cgattatctg tgtaaacctt
    36721 ttgcgttgat agaagtcgcc gccaggctgg aggctctgat gcgccgaacc aacggccagg
    36781 ccagcaacga gctgcgccac ggtaacgtca tgctcgaccc cggcaaacgt atcgccacgc
    36841 tggctggcga acccttaaca ctgaaaccaa aagaatttgc cctgctggaa ttactgatgc
    36901 gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat acctgggacg
    36961 aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc aaactcggta
    37021 gtgattttat tcgtaccgtg catggtattg gctacacatt aggtgagaaa tgaaatttac
    37081 ccaacgtctt agtctgcgcg tcaggctgac gctaatcttt ttaattctgg cctcggtgac
    37141 ctggctgctt tccagctttg tcgcctggaa acaaacaacg gataacgtcg atgaattgtt
    37201 cgacacccaa ctgatgctgt ttgccaagcg gttaagtacg ctcgatctca acgaaatcaa
    37261 cgcggcggat cgcatggcac agacgccaaa taaattaaaa cacggtcatg ttgatgacga
    37321 tgcgctgacc tttgccatct ttacccacga cggcagaatg gtccttaatg atggcgataa
    37381 cggagaagat attccctata gctatcaacg ggaaggtttt gctgacgggc aactggtcgg
    37441 tgaagacgat ccttggcgtt ttgtctggat gacctcacct gatggcaaat atcgcatcgt
    37501 tgttggccag gaatgggaat accgtgaaga catggcgctg gcgattgttg ccgggcaatt
    37561 gatcccgtgg ctggtcgcac tgccgattat gttaatcatc atgatggtac tactgggtcg
    37621 tgaactcgcg ccgctgaaca aactggcgct ggcactacgt atgcgtgacc ctgactcgga
    37681 aaaaccacta aacgcgactg gcgtacccag cgaagtgcga ccactggttg agtcgctaaa
    37741 tcaactgttc gcccgcacac atgcgatgat ggttcgagaa cgacgcttta cctccgatgc
    37801 agctcacgaa ctacgtagcc cgttaacagc gctgaaagtg caaaccgaag ttgcgcagct
    37861 ctctgacgat gatccgcagg cgcggaaaaa agcactgctc caattacatt ccgggatcga
    37921 tcgcgctact cgtctggttg atcaactgct cacgctatcg cggctggact cactggataa
    37981 ccttcaggac gtcgcggaga tcccgcttga agatctcctg caatcgtcgg tgatggatat
    38041 ttaccacacg gcgcagcagg cgaaaattga cgtgcgactg acactcaatg cccacagcat
    38101 caaacgcact ggacaaccgc tattgctaag tttgttggtg cgaaaccttc tggataacgc
    38161 cgtgcgctac agtccacagg gcagcgtggt ggacgtcacg ctgaatgccg ataatttcat
    38221 cgtgagggat aacggccccg gtgtgacacc agaggcactg gcgcgaattg gcgaacgctt
    38281 ctatcgcccc cccggacaaa ccgctaccgg cagtggactt gggctatcga ttgtccagcg
    38341 aatcgccaaa ttgcatggca tgaatgttga atttgggaat gcggaacaag gtggatttga
    38401 ggcgaaggtg agctggtaag ggtaagactt ttgctaaatt cgcaaaagac tttgcacatt
    38461 ttgctaattt taccgtaccg ctctgtgacg tactatagtc gacaaacgtc tcaccttgag
    38521 gttaaaaaat gagcaacacc ctgattatca acggcgcgaa aaaattcgcc cactccaatg
    38581 gtcaactgaa cgacaccctg accgaagtcg cggatggcac actgcgcgac ctcgggcatg
    38641 atgttcgcat cgttcgcgcc gacagcgact acgatgtcaa agcggaagta caaaactttc
    38701 tctgggctga tgtggtgatc tggcagatgc caggctggtg gatgggcgcg ccgtggacag
    38761 tgaaaaaata cattgatgat gtattcaccg aaggtcacgg gacgctgtat gccagcgatg
    38821 gccgtacccg caaagatccg tcaaaaaaat acggttccgg cggcctggta cagggcaaaa
    38881 aatatatgct ttctctgacc tggaacgcac caatggaagc cttcaccgaa aaagatcaat
    38941 tcttccacgg cgttggcgtc gacggtgtgt atctgccgtt ccataaagca aaccaattcc
    39001 ttggcatgga accgctgccg acatttatcg ctaatgacgt gataaaaatg cctgatgttc
    39061 cccgctatac tgaagaatat cgcaagcatc ttgtggaaat ttttggttaa ctagagctca
    39121 ggctttagaa ggagttaacc atgcttaccg taatcgcaga aatccgtact cgtcctggtc
    39181 aacatcaccg tcaggcggta ttggatcagt ttgctaaaat cgttccaacc gtactgaaag
    39241 aagaaggttg ccacggctat gcgccaatgg tggattgcgc tgctggcgtg agtttccagt
    39301 ctatggcacc ggattctatc gtgatgattg agcagtggga aagcatcgcg caccttgaag
    39361 cgcatctgca aaccccgcac atgaaggcgt acagcgaagc cgtaaaaggc gacgtgctgg
    39421 agatgaatat tcgtattctg cagccaggga tttaatcctg ccttgtttgc ccggccatcc
    39481 tgaccgggca atgttctttc ctttaaacct caatctccgc catgtcgcct ttctcttgca
    39541 accagttgcg gcgatcttcc gagcgtttct tcgccagcag catatccatc atcgcgtcag
    39601 tacgctgatc gtcttcatca tcgatagtca actgcaccag acggcgagtg ttcggatcaa
    39661 gcgtggtttc acgcaattgc atcgggttca tttcccccag acctttaaaa cgctggacgt
    39721 tcggcttgcc tttcttgcgt tttaattgct caagtacgcc ctctttctct tcttccgtca
    39781 gcgcgtaata aacctctttc ccgagatcaa tacggtagag cggtggcagt gcgacgtaaa
    39841 cgtgaccgtg tttcaccaac gcgcggaagt gttttacgaa caaagcgcag agcagcgtgg
    39901 caatgtgcag accatcagag tccgcatccg cgaggataca gattttgcca taacgaagct
    39961 ggctcagatc gtcgctgtca ggatcgatac cgatcgctac cgaaatatca tgcacttcct
    40021 gcgaggccag cacttcgtcg gaagaaactt cccaggtgtt aaggatctta cctttcagcg
    40081 gcatgatcgc ctgatattca cgatcgcgcg cctgcttggc agatccgcct gcggagtcac
    40141 cttccacgag gaacagttcg gtacggttaa ggtcctgcgc ggtacaatca gccagtttgc
    40201 caggcagcgc cgggccgctg gtcagctttt tacgcaccac ttttttagcc gcacgcatac
    40261 gacgctgggc gctggaaatc gccatctctg ccagcaactc cgccgcctga acgttctggt
    40321 tcagccacag gataaaggca tctttcacca cgccagaaac gaatgccgcg cattgacgcg
    40381 aagagagacg ctctttcgtc tgcccggcaa actgcggatc ctgcattttc accgacagaa
    40441 cataggcgca gcgatcccag atatcttccg ccgacagctt cacgccgcgc ggcagaatgt
    40501 tgcgatattc acagaactca cgcatcgcgt ccaacaggcc ctgacgcagg ccattaacat
    40561 gggtaccgcc ctgcatcgtt gggatcaggt tgacgtagct ttcggtcagc agttcaccgc
    40621 cttccggcag ccacagtagc gcccagtcca cagcttcagt atcgccagcg aaattaccga
    40681 taaacggttt ttctggcagc gtcggtaaac cgtttaccgc ttccgccagg taatcattca
    40741 gaccgtcctg atagcaccag cgctgttcgg tgttgttgat ctcatcttta aaggtaatct
    40801 caacgccggg gcacagtacc gctttggctt tcagcacatg cgtcaggcgt gaaacagaaa
    40861 atcgcgggct gtcaaagaag gtttcatccg gccagaagtg cacgctggta ccggtattgc
    40921 gtttaccgca agtgccgaca acctgtaaat cctgcacctt ttcgccattt tcaaaggcga
    40981 tgttataaac ctgaccatcg cggcgcacgt taacttcaac gcgcttcgac agggcgttaa
    41041 ccaccgaaat ccccacgcca tgcaggccgc cagagaactg gtaatttttg ttagagaatt
    41101 taccgcctgc atgcagacgg caaagaatca attcaaccgc cgggacaccc tcttccgggt
    41161 gaatatccac cggcatcccg cgcccatcgt caataacttc taacgactgg tcagcatgta
    41221 agattacatc cacgcgtttt gcgtgacccg ccagtgcttc atcgacactg ttatcaataa
    41281 cttcttgccc caaatggtta gggcgagtgg tatcggtata catccccgga cggcggcgaa
    41341 ccggctcaag cccggtgagt acctcaatgg catcagcgtt ataagtttgc gtcatggttt
    41401 aagttagcaa ttcgagttga tcgtcagaga tggtgcagac caagaaaatc gacgatcggg
    41461 ttgaaataat cttcgaagcc cgtgaatgcg tggttgccgc cttctatgac agtctggcgg
    41521 caggaagcgt agtacgccac cgcctggcgg taatccagca cttcatctcc cgtctgttgc
    41581 agcagccaga tcaaatccgg cgcttccagc gggtcaatct gcatgacttt gagatcgtaa
    41641 atatggcgtg actctagcac atattgctgc ccggtgtatg ggttctcgtt ctgaccgaga
    41701 tagtccgtca gcagttcaaa cgggcgcacc gccgggttta ccaccactgc gggcagcata
    41761 aaacattgtg acaaccaggt ggcgtaatat ccccccagtg acgaaccgac aatacccagc
    41821 gaatcgccgc catgttccag gacaatggat tccagcagtt ctgccgcgtc ggaaggatac
    41881 ggcggcaact gcggaatgat catctcaacg tcagggtgat gttccgccag ccagtttttt
    41941 aacaagctcg cttttgcaga gcgcggcgag ctgttgaaac cgtgtaaata aagaagcgta
    42001 gacatcagta gccttctgaa gcggtatcag gttggaaacg tgtgtccgcc aggcgatgca
    42061 cctcggtggt cagcgtgcca tcagcatgta actcgagagt acgccagccg ggcgcgatag
    42121 tatccagcgt aaagttggaa cagtgcggct taaactgcac acaggtcgac ggcgttgcca
    42181 gcaggcggcg accgttccag tcgagatcca gctcctgatg aatatgaccg cacagcaagt
    42241 atttgacgtg cggaaacttc gccagcacgg tatccagttc gcccgcgtta cgcagactgt
    42301 gttgatcgag ccaactacaa cccgcaggta gcggatgatg atgcagcagc agcaacgtat
    42361 ggcgttctgg cgcatcggcc agtttacgtt ccagccactc aagctgaaac tcgctcagct
    42421 caccgtgcgg cacgccaaac acctggctat ccagcaacag gatttgccat tgctcaccaa
    42481 taaacacccg cttcgccggg gagatacccg catcctgtaa cgcgctgtac atcgcgggct
    42541 ggaaatcgtg gttgcccggc agccagacgc agggcgcacg aaaacttgcg atgccttcag
    42601 cgaaatgctg ataggccgca gaggattgat cctgcgctaa atcacctgtc gcgacaatca
    42661 ggtcgaattc gtgctgatgt ggacgaatcg cctccagcac cgcctggtag ctttcccagg
    42721 tgtttacccc taacagagct tcgtgctttt gtgcaaacag gtgagtgtcg gtaatttgta
    42781 aaatcctgac tctggcctca ccagccagag gaagggttaa caggctttcc aaatggtgtc
    42841 cttaggtttc acgatgctag taaaccggaa tcgccatcgc tccatgtgct aaacagtatc
    42901 gcaaccagtc cgctaaaaac tgattaattt gatgcttttc gtcgcgttga tgcaactttt
    42961 tattaggata atcataccgc gctttgaagc gaaaaatctg ctggcttgaa cacacttcag
    43021 ccaccatcgc gtcatgatac agacgcaccg tcattgacgg aaggctccag taactgatcg
    43081 cgggcgcagt ctgttctatt gtcaccaggg tagtgtatcg ggtcgattcc acaatcgtca
    43141 gccgatattg tgcgtttgcc acctgatagc ttacagtttc gccgggtgcg tcattgcgcg
    43201 gtaacaaacg gcgcaattgt gaaaagttca tctcgcacag gcgcatcatt tcaggaaagt
    43261 caggtgtgta acgcttcatt tatgcccact cattttttaa cgcttgatga tgcagctgca
    43321 gccattgcaa agcgatgacc gacgctgcgt tgtcgatttt cccctcttct acccactggt
    43381 atgcctgttc ccggcttacc acatgaacgc gaatatcttc gttttcatca gccagaccat
    43441 gaataccgct tgcggtcgtg gcgtccactt cgcccaccat aattgacgaa cgctcactgg
    43501 tgccccccgg gcttgccagg aaacttaaca ccggtttggt ccgtttgact atcagtcccg
    43561 cctcttcaat cgcttcgcga cgggcaacat cttccacact ttcaccctct tctatcatcc
    43621 cggcaaccat ctccagtagc cagggggttt cactggtgtc gtatgcggca atccgaatct
    43681 gctcaatcag cacaacttca tcgcgcactg ggtcaaaggg tagcaagact gcggcgtgac
    43741 cgcgctcaaa aatttcccgc cgtacctcat gactcatttg cccgttgaat agacgatgac
    43801 gaaatctata aagatctaat gaaaaaaagc cgcgataaag tgtttctcgt gcaataattt
    43861 ctacatcgtt tttgccaaat gtaacgggca ggttgtctgg cttaagcatt gttaatgtcc
    43921 tggcactaat agtgaattaa atgtgaattt cagcgacgtt tgactgccgt ttgagcagtc
    43981 atgtgttaaa ttgaggcaca ttaacgccct atggcacgta acgccaacct tttgcggtag
    44041 cggcttctgc tagaatccgc aataatttta cagtttgatc gcgctaaata ctgcttcacc
    44101 acaaggaatg caaatgaaga aattgctccc cattcttatc ggcctgagcc tttctgggtt
    44161 cagttcgttg agccaggccg agaacctgat gcaagtttat cagcaagcac gccttagtaa
    44221 cccggaattg cgtaagtctg ccgccgatcg tgatgctgcc tttgaaaaaa ttaatgaagc
    44281 gcgcagtcca ttactgccac agctaggttt aggtgcagat tacacctata gcaacggcta
    44341 ccgcgacgcg aacggcatca actctaacgc gaccagtgcg tccctgcagt taactcaatc
    44401 catttttgat atgtcgaaat ggcgtgcgtt aacgctgcag gaaaaagcag cagggattca
    44461 ggacgtcaca tatcagaccg atcagcaaac cttgatcctc aacaccgcga ccgcttattt
    44521 caacgtgttg aatgctattg acgttctttc ctatacacag gcacaaaaag aagcgatcta
    44581 ccgtcaatta gatcaaacca cccaacgttt taacgtgggc ctggtagcga tcaccgacgt
    44641 gcagaacgcc cgcgcgcagt acgataccgt gctggcgaac gaagtgaccg cacgtaataa
    44701 ccttgataac gcggtagagc agctgcgcca gatcaccggt aactactatc cggaactggc
    44761 ggcgctgaat gtcgaaaact ttaaaaccga caaaccacag ccggttaacg cgctgctgaa
    44821 agaagccgaa aaacgcaacc tgtcgctgtt acaggcacgc ttgagccagg acctggcgcg
    44881 cgagcaaatt cgccaggcgc aggatggtca cttaccgacg ctggatttaa cggcttctac
    44941 cgggatttct gacacctctt atagcggttc gaaaacccgt ggtgccgctg gtacccagta
    45001 tgacgacagc aatatgggcc agaacaaagt gggcctgagc ttctcgctgc cgatttatca
    45061 gggcggaatg gttaactcgc aggtgaaaca ggcccagtac aactttgttg gtgccagcga
    45121 gcaactggaa agcgcgcatc gtagcgtcgt gcaaaccgta cgttcctcct tcaacaacat
    45181 taatgcatct atcagtagca ttaacgccta caaacaagcc gtagtttccg ctcaaagctc
    45241 attagacgcg atggaagcgg gctactcggt cggtacgcgt accattgttg atgtgttgga
    45301 tgcaaccacc acgctgtaca acgctaagca agagctggca aatgcgcgtt ataactacct
    45361 gattaaccag ttgaatatta agtcagctct gggtacgttg aacgagcagg atctgctggc
    45421 actgaacaat gcgctgagca aaccggtttc cactaatccg gaaaacgttg caccgcaaac
    45481 gccggaacag aatgctattg ctgatggtta tgcgcctgat agcccggcac cagtcgttca
    45541 gcaaacatcc gcacgcacta ccaccagtaa cggtcataac cctttccgta actgacgacg
    45601 acgacggggc ttcggccccg tctgaacgta aggcaacgta aagatacggg ttatctgccg
    45661 cattcttccc ccttctcgct tcaatttcga ccagccatcc tctattctga tgggtattta
    45721 ccactggtcc cggaagacaa aaatgaaacg gacaaaatcc atacgccacg catcgttccg
    45781 caaaaactgg agcgcacgcc atctgacacc agtcgctctc gcggttgcca ctgtttttat
    45841 gctggctggc tgtgaaaaga gtgatgaaac agtgtctctc tatcaaaatg ctgacgactg
    45901 ttcagctgca aacccaggca aaagcgccga atgtaccacc gcgtacaaca atgcgctgaa
    45961 agaagccgaa cgtactgcgc cgaaatacgc cacccgtgaa gactgtgttg ctgaatttgg
    46021 tgaaggtcag tgccagcagg caccagccca ggctggcatg gcaccagaaa accaggcgca
    46081 ggcccagcaa tccagcggga gtttctggat gccgctgatg gccggttaca tgatggggcg
    46141 tctgatgggc ggcggcgcgg gatttgcaca gcagccgctg ttctcctcga aaaacccagc
    46201 cagcccggct tacggtaaat ataccgacgc gacgggtaaa aactatggcg cagcccagcc
    46261 aggccgcacc atgaccgtac cgaagacagc catggcacca aaaccggcga ccactactac
    46321 cgttacccgt ggcggttttg gtgaatctgt tgccaaacaa agcactatgc agcgtagcgc
    46381 aaccggcacc tcttctcgtt caatgggtgg ctgataccga tggaaagagt cagtattacc
    46441 gagcgcccgg actggcgcga aaaagcccac gaatacggtt tcaattttca caccatgtac
    46501 ggcgagccgt actggtgtga agatgcttac tacaagttga ccctcgccca ggttgaaaag
    46561 ctggaagaag tcaccgccga actgcaccag atgtgcctga aagtggtgga aaaagtgatc
    46621 gccagcgatg agctgatgac caaattccgc attccaaaac gcacctggag ttttgtgcgt
    46681 cagtcatggc tgactcacca gccatcgctt tattcgcgtc ttgatctggc atgggatggc
    46741 actggcgaac ctaaactgct ggaaaataac gccgatacac caacgtcact atacgaggcg
    46801 gcgttctttc agtggatctg gctggaagat cagcttaacg ccggtaactt gccggagggc
    46861 agcgaccagt ttaacagtct gcaagaaaag ctaatcgatc gctttgtcga gttgcgtgaa
    46921 cagtatggct tccagttgct gcatctcacc tgctgtcgcg acacggtgga agatcgcgga
    46981 accattcagt atttgcagga ctgcgcgacg gaagctgaaa tcgctaccga gttcctctac
    47041 attgacgata tcgggttagg tgaaaaaggt cagttcacgg atttacagga tcaggtaatt
    47101 tccaacctgt tcaagctgta tccatgggaa tttatgttgc gtgagatgtt ctccaccaag
    47161 ctggaggatg caggcgtacg ctggctggaa ccggcgtgga agagcattat ctccaacaaa
    47221 gcgcttctac cgctactgtg ggagatgttc ccgaatcacc cgaacctgct gcccgcttat
    47281 tttgcggaag atgatcatcc gcaaatggaa aaatatgtgg ttaaaccgat cttctcccgt
    47341 gaaggcgcaa acgtgtcgat cattgagaac ggcaaaacca ttgaagcagc ggaaggtccg
    47401 tatggcgaag aagggatgat tgttcagcaa ttccacccgt taccgaaatt cggcgacagc
    47461 tatatgctga ttggtagctg gctggtgaac gatcaacctg ccggaattgg cattcgtgaa
    47521 gaccgtgcgt tgatcaccca ggatatgtct cggttttatc cacatatttt tattgagtaa
    47581 tcaatgatgc cggatggcat tcgccatccg gtaattgtta acctatctgc accgacagca
    47641 tactcaggct gcccatttct ataccatcga ccggaatggt aattggctcc tgcccatccc
    47701 acgcacctaa gacatacaac aacggcagat agtgctccgg cgttgggttc gataacgcgc
    47761 caccttcatg gtcaaggtaa ttcaccagag gatgctgctc taccgcccct tgccacgtca
    47821 gattcgcttt cacatactca ttaaacgaca tcgcccaagg atatggtgaa ctatcaccgt
    47881 gccacttcac tgtacgcagg ttatgcacca cgttgccact ggcgaccaac attattcctt
    47941 catctcgcag cgctgccagt ttgcgcccca tttcgaaatg ccaggcggca ggtttgctac
    48001 tgtcgatact caactgcacc atcgggatat cggcgtcagg atacatctta atcagcacgc
    48061 cccaggaacc gtggtcaaag ccccaggctt ctttatccag cgtcaccggg accgacgcta
    48121 acagctcaac cagatgctgt gccagcacag gcgaacccgg agcaggataa tgcgtatcgt
    48181 acagcgcctg cgggaagcca ccaaagtcat gaatcgtggg cggcgtctcc atcgcggtca
    48241 ctcctgttcc acgggtaaac cagtgagccg aaaccaccac aatcgcttgc gggcgtggca
    48301 atgtcatccc caacttctgc cagctgcggg tatacaaatt atcttccagc acgttcatcg
    48361 gactaccgtg acctaaaaac aatgctggca tacgtgttga agacatgatg atatccttaa
    48421 ctaaaggtgt cattttgata tcctcacaat acgcttgttc ggcggagtaa gaacccggat
    48481 aacaatgatg atgatcatca gttattttga cgatctgcct gaaggtgaag atttataagg
    48541 agttgtcgat gtcagtacct ctcattctga ccatactggc gggggcagcc acgtttattg
    48601 gcgcgtttct cggcgttctc gggcaaaaac cctcgaaccg cttactggcg ttttcgctag
    48661 gatttgcggc ggggatcatg ttgctcatct cattaatgga aatgcttcct gccgcactag
    48721 cagctgaagg aatgtcgcct gtgttgggtt atggaatgtt tatcttcggt ctgcttggct
    48781 attttggcct ggaccgcatg ttgccacatg cgcatccgca ggatttaatg caaaaatcgg
    48841 tgcagccgtt gccaaaatcg atcaagcgca cagccattct gctcactctc ggcatcagtc
    48901 tgcataactt cccggaaggg attgccacct ttgtcacggc gagcagcaac ctggagctgg
    48961 gatttggcat cgcactggcc gtcgcgttgc acaatatccc tgaaggtctg gcagtggcag
    49021 gcccggttta tgcggcaacg gggtctaaac gcaccgcgat tctgtgggcg gggatttctg
    49081 gactagcaga aattcttggt ggtgtgctgg cgtggttaat cctcggtagc atgatttccc
    49141 cggtggtcat ggcggcaatc atggcggcgg ttgcaggaat tatggtggcg ctctcggttg
    49201 atgaattaat gccgctcgcc aaagagattg accctaataa taaccccagc tatggcgtac
    49261 tatgtggaat gtcagtgatg ggattcagtt tagtgctgct acaaacggcg ggaattggtt
    49321 aaaaacaaca tcagaagttg tccagcacat ggacaacttc tgccagcttc acgaaagcct
    49381 tattctacct tttcggctgt ctcttctctc gtactgttta agtatttgtt ccgcgcttca
    49441 agcagtgcgt ctctgtcctg gcgaaacgcc cgacaataac gtttcaaatg caggaaataa
    49501 cccaccacaa ccagagcaat caatacaatc cagtaccagg cgataaacat ccttttaccc
    49561 ttaatattta acaatactaa acatttagcg cataaatttc acatatcctt tttcggatat
    49621 attcatatgg tcgcataata acggaacaat tatatgattt tgatcataat gaaaaagaat
    49681 gcctgatatt aatgaaattt tctcatagaa agagaagcgg aattaacatc ttgcatagca
    49741 gggcaaaata aaaaccgaag ccagctacaa cttcggtttc ttgattaagg cagtaattaa
    49801 gcagcggttt tcagctggct ttacgctcat gtgcctgacg gtatgccacc aggtcttcaa
    49861 tagtcacgag cgccatattg tgtttattgg caaactcaat gcactctggt gcacgcgcca
    49921 tcgtgccatc gtcattagtc agctcacaca gcacaccagc cggtttaaag cctgccagcg
    49981 tcatcaaatc aatagtcgct tcagtatgac cgccacgcgt cagtacacca cctgcctgcg
    50041 cgcgaagtgg gaaaacgtgg cccgggcgat tcagatcaga aggttttgcg ccatcggcaa
    50101 tcgctgcgcg aacggtcgta atacgatcag cggcagaaac accggtagtc acaccttcag
    50161 ccgcttcaat ggtcacggta aaaccggtgc cataggcgct ggtgttattt tctaccatca
    50221 ttggcagatc aagttgttta cggcgatctt cagtgatgca caggcaaaca ataccgctac
    50281 cgtggcgaat ggtcagcgcc atctgctcaa cggtcatggt ttctgccggg aagatcatat
    50341 cgccttcgtt ttcacggtct tcatcatcaa gcaccattac accgcgtcct tcacgcagcg
    50401 cagccagtgc attttcaaca cgttcgaaag gcgtaccaaa agaggaaagt agcgtctgat
    50461 tcatggtaaa aaaacctcac taaaattatg gttaccagaa tcagggcagt cttaggagtg
    50521 gcggcatata gccaaaataa cgtgagcggg tccatgcccg acagaatcgt tactctctcc
    50581 catccggact ctaaccgtcg gccccggaat tacaccggat ctgctgtcct ttgagttcgc
    50641 acccaaagcg ctcgcgggct ttcaactgag ttgatttacc gccggtgggg aatttcgccc
    50701 cgccctgaga ataagcggat tcactataac gctaatgatt agcggcagca acgcatagct
    50761 tcacataatt ctggtttatg acttaccctt atcgcactac aatggtactc aacacctatc
    50821 agtacaggga aactacaatg attgacccga aaaaaattga gcaaatcgct cgccaggttc
    50881 acgaatcaat gcctaaagga atcagggagt tcggggaaga tgtggagaaa aaaatccgcc
    50941 aaaccctaca agctcagctg acgcgcctcg accttgtaag ccgtgaagag ttcgacgtcc
    51001 aaacgcaagt cctgttacgg actcgtgaga aactggcgcg gcttgaacaa agaatgagcg
    51061 aactggaaaa tcgcagtact gagattaaaa agcagccaga tccagagact ctccccccaa
    51121 cgctgtaaac atttgtcttt aatgcactgt aaattatgaa ttaatcttct aatttacagt
    51181 gctttctatt accttatcgc ttaattccat tcagaatatc atcacaaaaa tgaatgagtg
    51241 caattgaaaa tataattgtt atgtattttt taagttttac ttaatccata tttagataaa
    51301 ctaatttcat aaaacccgaa aagtaaaata taatatttga atgattagca atccttaaat
    51361 aacagttact aatcattcat tttctactta tatttatata aaggaatatt atgtctgctt
    51421 ttaaaaagtc acttcttgtt gctggcgtag caatgattct ttccaataac gtatttgctg
    51481 acgaaggtca cggcattgtt aaatttaaag gtgaagttat ctctgctcca tgttcaatta
    51541 aaccaggtga cgaagactta actgtaaatc tgggtgaagt agcagatacc gttctgaaaa
    51601 gcgaccagaa atctttggct gagcctttca ctattcacct gcaagactgc atgttgagcc
    51661 agggtggaac cacttattca aaagcgaagg ttactttcac cacagcgaat actatgacgg
    51721 gtcagactga cctgctgaaa aacaccaaag aaactgaaat cggtggtgcc acaggcgttg
    51781 gcgtacgtat tctggatagc cagagcggcg aagtgacttt aggtactccg gtagtaatta
    51841 cattcaacaa tactaacagc tatcaggaac tgaacttcaa agctcgtatg gaatccccgg
    51901 gcaaagatgc taccccgggc aacgtttacg ctcaggctga ctataagatt gcttacgagt
    51961 aatcacatta ggccgggttc gcccggccac atcattaacg gattaatgat aagtggaaca
    52021 gatgtataaa aaattaaaat taaccacaat aagcgaattg attaaaaata tttattgttc
    52081 attatccgtt ctcatcattg gttgtgcgtc agcttatgcc gttgaattca acaaagattt
    52141 aatcgaagcc gaagatcgtg aaaacgttaa cctttcccaa tttgaaactg atggccaatt
    52201 acccgtaggt aaatactcat taaacgctct tattaataat aaaaggacgc caatccatct
    52261 ggacctgcaa tgggtattaa ttgataacca aactgcagtt tgtctgactc cagagcaatt
    52321 aacattatta ggctttactg atgagattat tgaagaggct cagcaaaacc tgatcgatgg
    52381 ttgttaccct atcgaaaaag aaaaacaaat tacaacttat ctcgataaag ggaaaatgca
    52441 attatccata tctgcacctc aggcatggtt aaaatacaaa gatgcaaact ggacgcctcc
    52501 tgaactttgg gatcatggta ttgctggggc atttcttgac tacaatttat atgcctctca
    52561 ttatgcacca catcagggcg ataattcaca aaatataagt tcctatgggc aggctggggt
    52621 taatcttggg gcctggcgcc tgcgtactga ttaccagtac gatcagtcat ttaacaatgg
    52681 caaaagccag gcgaacaacc tggattttcc gcgtatttat ttgtttcgcc caatcccagc
    52741 aattaatgca aaactaacta taggtcaata cgatactgaa tcctctattt tcgactcttt
    52801 ccatttttct ggcgtttcgt tgaaaagcga tgagaatatg ttgccgcctg atctacgtgg
    52861 ttacgcaccg caaatcacgg gtgttgcaca aacgaatgca aaggtcactg tctcacagaa
    52921 caaccgtatt atttatcaag aaaatgttcc tcctggccca tttgctatta ctaatttatt
    52981 caatacatta caggggcaac tggacgtcaa ggttgaagaa gaggacgggc aagtcacgca
    53041 atggcaagtt gcatctaata gtattcctta tctgacgcgt aaagggcaga ttcgctacac
    53101 cactgctatg ggtaaaccga ccagcgttgg tggtgattcc ttacaacaac ccttcttctg
    53161 gactggtgaa ttctcatggg gttggctgaa caatgtatcc ctgtatggtg gttcagtttt
    53221 aacaaaccgt gattatcaat ctctggctgc cggcgttggc tttaacctta actcattggg
    53281 ttcattatct tttgatgtca cacgatctga tgctcagttg cataatcagg ataaagaaac
    53341 gggttatagc taccgcgcta actattcaaa acgttttgaa tctaccggta gccagctcac
    53401 tttcgctggt taccgtttct ctgataaaaa ctttgtgaca atgaatgaat atatcaatga
    53461 cactaaccat tacatgaatt atcagaatga aaaagagagt tatattgtca cgtttaacca
    53521 gtatcttgaa tcattaaggt taaatacata cgtaagtttg gctcgtaata cttactggga
    53581 cgccagcagt aatgtgaatt attcattatc acttagccgc gattttgata tcgggccatt
    53641 aaaaaacgtc tccgcttctc taacatttag ccgaataaac tgggaagaag acaaccagga
    53701 tcaactgtac ctaaatattt cgattccctg gggaaccagt agaacattga gctatggtat
    53761 gcaacgaaat caggataaca agatttcgca tactgcttcg tggtatgact cttccgatcg
    53821 aaataattcc tggagcgttt ctgcttcagg cgacaatgat gaattcaaag atatgaaagc
    53881 gtcactacgc gccagttatc agcataatac cgagaacggt cgtctctatc tctccgggac
    53941 atcacagcga gacagttatt attctctgaa tgccagttgg aatggttcat tcacggcgac
    54001 tcgtcacgga gccgctttcc atgactatag tggtagtgct gactcgcgtt ttatgatcga
    54061 cgcagatggt gcagaagata ttccgttgaa caataaacgc gcggtaacta atcggtatgg
    54121 catcggagtt attccatcag tcagcagtta cataacaaca tcattaagcg ttgatacccg
    54181 aaatcttcca gaaaatgtgg atatcgaaaa ctcggttatc accaccacct taaccgaagg
    54241 tgctattggc tacgccaaac ttgatacccg caagggctat caaatcatgg gggttattcg
    54301 cctggcagat ggtagtcacc caccactggg gattagcgta aaagataaaa ccagccataa
    54361 agaattagga ctggttgctg atggcggctt tgtatacctc aacggcattc aggatgacag
    54421 caaacttact ttacgctggg gtgataaatc ctgttttatt caaccaccca atagcagcaa
    54481 cttaaccacc ggaacggtta ttttaccgtg tattagccaa aattaattca ggatgaaact
    54541 aatgcgctac ttaaacacaa agaatctcat tgcagcagga gtattactcg cctgcatgag
    54601 cagcattgcc tggggtgcca tcatcccgga ccgtacccgt atcataatga atgagtcaga
    54661 taaaggggaa gcattaaaac ttactaatca aagcaaaaat ctaccctatc tggcgcaaac
    54721 atggatagaa gatacgaaag gcaataaatc acgtgacttt attgtcacgg ttccccctat
    54781 ggtacgttta aatcccagtg agcaaattca aataagaatg ataagtcagg aaaaaattgc
    54841 tcaactgcct aatgacagag aaacgctctt ctattttaac gtgcgagaaa taccgccaaa
    54901 aacagataaa aaaaatgtga tgcaagtcac tatgcagcac gcattgaaac tattctggcg
    54961 accaaaagct attgaattag aagatgatgg ggtaatgtct tacgaaaaag ttgaaatcat
    55021 ccgtagaaat gatggttcga tccgctttaa caataagatg ccttatcacg ttacgctggg
    55081 ttatatcgga acaaacggaa tcacaatgtt gccacaaacg caaagtttaa tggtaacccc
    55141 ttatagccat gcagatacgc agtttaaaaa tgttccgtca gcttttcagg ttggttatat
    55201 caatgatttt ggtggattaa gtttttatga aataaactgc ccgacggtta ataactcttg
    55261 taatgtttcc gtagccaaac gagataaata atatgcgcta cttattaatt gtaattatat
    55321 ttattatagg ttttagtgca ttacctgtat gggcaatgga ctgctatgct gaacatgaag
    55381 ggggaaatac cgtagtcatt ggttacgtac caagaatttc tatccccagt aatggtaaaa
    55441 aaggtgataa gatctggcaa agcagtgagt attttatgaa tgtcttctgt aataatgcat
    55501 tacctgcccc atcacctgga gaagaatacc catcagcctg gactaatata atgatgtttt
    55561 taccaggcgg tcaggacttt tataatcaga actcctatat ttttggcgta acatacaatg
    55621 gagttgatta tgattcgact gcccctaatg ctatcgcagc accagaatgc attgatataa
    55681 aaggagcggg tacctttaat aatcactata aaaaccccgc tgtctgtagt ggaggtcctg
    55741 agcctcaatt atcagtaacc tttccagctc gcgtacaact ctatattaaa ttagcaaaaa
    55801 atgccaatag agttaataaa aacctggtgt tacctgatga atatatcgct ctagagttta
    55861 aaggtatgag tggcgcagga gctatagaag tagacaaaaa tttgaccttc aggattcgtg
    55921 gattaaataa tattcatgtc cttgactgct ttgttaatgt cgcactggaa ccagctgatg
    55981 gcgttgttga ctttggtaaa ataaattccc gaacaattaa aaataccagc gttagtgaga
    56041 cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa ttcaatattt
    56101 tagggagctt tttcactacg gatattttga gtgattatag ccatctggat atgggtaatg
    56161 gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac cgcttctcac
    56221 aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc agggcagaac
    56281 ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac gtaatcctca
    56341 aaatcaccta t
//
LOCUS       DAQSWQ010000015        55730 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.015, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000015 DAQSWQ010000000
VERSION     DAQSWQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.015"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="R8G18_000935"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="R8G18_000935"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEE9794719.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="R8G18_000936"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="R8G18_000936"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HEE9794720.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="R8G18_000937"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="R8G18_000937"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEE9794721.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="R8G18_000938"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="R8G18_000938"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEE9794722.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="R8G18_000939"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="R8G18_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HEE9794723.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="R8G18_000940"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="R8G18_000940"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEE9794724.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="R8G18_000941"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="R8G18_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HEE9794725.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="R8G18_000942"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="R8G18_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HEE9794726.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="R8G18_000943"
     CDS             6537..6773
                     /locus_tag="R8G18_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEE9794727.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="R8G18_000944"
     CDS             6776..7633
                     /locus_tag="R8G18_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEE9794728.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="R8G18_000945"
     CDS             7654..9645
                     /locus_tag="R8G18_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEE9794729.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="R8G18_000946"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="R8G18_000946"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HEE9794730.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="R8G18_000947"
     CDS             complement(10248..11144)
                     /locus_tag="R8G18_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEE9794731.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="R8G18_000948"
     CDS             complement(11193..11432)
                     /locus_tag="R8G18_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEE9794732.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="R8G18_000949"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="R8G18_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HEE9794733.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="R8G18_000950"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="R8G18_000950"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HEE9794734.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="R8G18_000951"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="R8G18_000951"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEE9794735.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="R8G18_000952"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="R8G18_000952"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEE9794736.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="R8G18_000953"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="R8G18_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="R8G18_000954"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="R8G18_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HEE9794737.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="R8G18_000955"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="R8G18_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HEE9794738.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="R8G18_000956"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="R8G18_000956"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HEE9794739.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="R8G18_000957"
     CDS             complement(21570..22739)
                     /locus_tag="R8G18_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9794740.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="R8G18_000958"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="R8G18_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HEE9794741.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="R8G18_000959"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="R8G18_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HEE9794742.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="R8G18_000960"
     CDS             complement(24315..25247)
                     /locus_tag="R8G18_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9794743.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="R8G18_000961"
     CDS             25360..26571
                     /locus_tag="R8G18_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HEE9794744.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="R8G18_000962"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="R8G18_000962"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEE9794745.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="R8G18_000963"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="R8G18_000963"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEE9794746.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="R8G18_000964"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="R8G18_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HEE9794747.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="R8G18_000965"
     CDS             complement(30320..31576)
                     /locus_tag="R8G18_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HEE9794748.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="R8G18_000966"
     tRNA            31884..31960
                     /locus_tag="R8G18_000966"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="R8G18_000967"
     tRNA            31965..32041
                     /locus_tag="R8G18_000967"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="R8G18_000968"
     CDS             32149..32454
                     /locus_tag="R8G18_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HEE9794749.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="R8G18_000969"
     CDS             32580..34184
                     /locus_tag="R8G18_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HEE9794750.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="R8G18_000970"
     CDS             complement(34196..35008)
                     /locus_tag="R8G18_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794751.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="R8G18_000971"
     CDS             complement(35012..35797)
                     /locus_tag="R8G18_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HEE9794752.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="R8G18_000972"
     CDS             complement(35794..36462)
                     /locus_tag="R8G18_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEE9794753.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="R8G18_000973"
     CDS             complement(36526..37164)
                     /locus_tag="R8G18_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HEE9794754.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39279)
                     /locus_tag="R8G18_000974"
     CDS             complement(37177..39279)
                     /locus_tag="R8G18_000974"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HEE9794755.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39300..39926)
                     /locus_tag="R8G18_000975"
     CDS             complement(39300..39926)
                     /locus_tag="R8G18_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HEE9794756.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40382..40591)
                     /gene="fumD"
                     /locus_tag="R8G18_000976"
     CDS             complement(40382..40591)
                     /gene="fumD"
                     /locus_tag="R8G18_000976"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HEE9794757.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            41148..42560
                     /gene="pykF"
                     /locus_tag="R8G18_000977"
     CDS             41148..42560
                     /gene="pykF"
                     /locus_tag="R8G18_000977"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HEE9794758.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42870..43106
                     /gene="lpp"
                     /locus_tag="R8G18_000978"
     CDS             42870..43106
                     /gene="lpp"
                     /locus_tag="R8G18_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HEE9794759.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43169..44173)
                     /gene="ldtE"
                     /locus_tag="R8G18_000979"
     CDS             complement(43169..44173)
                     /gene="ldtE"
                     /locus_tag="R8G18_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HEE9794760.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44322..44738)
                     /gene="sufE"
                     /locus_tag="R8G18_000980"
     CDS             complement(44322..44738)
                     /gene="sufE"
                     /locus_tag="R8G18_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HEE9794761.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44751..45971)
                     /gene="sufS"
                     /locus_tag="R8G18_000981"
     CDS             complement(44751..45971)
                     /gene="sufS"
                     /locus_tag="R8G18_000981"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HEE9794762.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(45968..47239)
                     /gene="sufD"
                     /locus_tag="R8G18_000982"
     CDS             complement(45968..47239)
                     /gene="sufD"
                     /locus_tag="R8G18_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEE9794763.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47214..47960)
                     /gene="sufC"
                     /locus_tag="R8G18_000983"
     CDS             complement(47214..47960)
                     /gene="sufC"
                     /locus_tag="R8G18_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEE9794764.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(47970..49457)
                     /gene="sufB"
                     /locus_tag="R8G18_000984"
     CDS             complement(47970..49457)
                     /gene="sufB"
                     /locus_tag="R8G18_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEE9794765.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49466..49834)
                     /gene="sufA"
                     /locus_tag="R8G18_000985"
     CDS             complement(49466..49834)
                     /gene="sufA"
                     /locus_tag="R8G18_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HEE9794766.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50382..50570)
                     /locus_tag="R8G18_000986"
     CDS             complement(50382..50570)
                     /locus_tag="R8G18_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HEE9794767.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50670..51080)
                     /gene="menI"
                     /locus_tag="R8G18_000987"
     CDS             complement(50670..51080)
                     /gene="menI"
                     /locus_tag="R8G18_000987"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HEE9794768.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51077..54133)
                     /locus_tag="R8G18_000988"
     CDS             complement(51077..54133)
                     /locus_tag="R8G18_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEE9794769.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54522..55634
                     /gene="ydiK"
                     /locus_tag="R8G18_000989"
     CDS             54522..55634
                     /gene="ydiK"
                     /locus_tag="R8G18_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEE9794770.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT        13415 a        14653 c        13644 g        14018 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaagcac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcaacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacaaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc
    38041 cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct tgaacacacc
    38101 tttgctgtaa caataggtaa agtcgcgatg caactgcccg tagttacacc ataggccgta
    38161 gtcatcgaac agattcagac ccaccaggtt accaatcaca cgaccatcgt ctttatcttc
    38221 aaaatctttc ggaccgttcg ggaagatggt ggtatggaca aagttggcta cacaagtgtt
    38281 accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca
    38341 acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg ccgccggacc
    38401 taagtcaaaa acggatttat aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat
    38461 ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt
    38521 ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg gcacgacatg
    38581 gttgttattc gcaccaataa gttcagtcat catgtaatcg ttgagacgct tcatctcctg
    38641 acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga
    38701 acccattatt gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga
    38761 gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt ccggactggt
    38821 caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt
    38881 ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc
    38941 gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg
    39001 ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga
    39061 actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc
    39121 atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc cgaagcccat
    39181 gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag
    39241 attgactctt aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt
    39301 cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg
    39361 cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc
    39421 atccacgggc atgccgtagt acaggcgcta cagccaatac agcgtttatg atcgacggta
    39481 atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc
    39541 ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg
    39601 ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag
    39661 aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc
    39721 gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg
    39781 ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt
    39841 agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct
    39901 aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga
    39961 caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac
    40021 cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat aaaccaatcc
    40081 tttatggtta ggagaaaaaa taatgtgatt attctgagcc cttaacattg atcgttatca
    40141 attaaaatta caaagcaaga aattataagt gaactgatat ttattatcaa ttgaaataaa
    40201 tttaacttaa gcgacaatta tttgtgacgt tcattgcaga aaataaaaat aatattatcg
    40261 ttatagatta aaatacatac cgcaattcgt ataatttcag cagttgttat ttagaatgat
    40321 taactggacc gcaactgaag caagaatatc aggatgaagc ttaccgcctc atcctgaatg
    40381 gtcattttac cagtgcgtta atgacggttt ccatcgcctg cttttccagc tcactgcgct
    40441 gaatgcgttt attggctaat gcagtacgta atacgcctgc gataacaata tgctttggtt
    40501 cctgccccag atcgcgcatt tccagcacaa ctttaccgac aactctgcac atttctcggt
    40561 acagttcatc ctcttttgtc cgatttccca ttctttatac ctatttatca tgccaactat
    40621 cagcatatat caatctaacc aattaaacaa aaatcaaaca aaatcagaca aataacgcga
    40681 taaattattt taactgtagc aattgagcga tgatatattt atacaccgga tgaactttca
    40741 cttatcctca cactgacaac ttcggcacca gacgttgcgc aaacagcgaa gtttttgcgt
    40801 aaccttttcc ctggaacgtt aaatctttga taacaattta ttgtctaaca agttgtatat
    40861 tttttgaaac gctgtttttg ttttcctttt ggattaattt cagcgtataa tgcgcgccaa
    40921 ttgactcttg aatggtttca gcactttgga ctgtagaact caacgactca aaaacaggca
    40981 ctcacgttgg gctgagacac aagcacacat tcctctgcac gctttttcga tgtcacctat
    41041 ccttagagca aggcaccacc actttcgtaa taccggattc gctttccggc agtgcgccca
    41101 gaaagcaagt ttctcccatc cttctcaact taaagactaa gactgtcatg aaaaagacca
    41161 aaattgtttg caccatcgga ccgaaaaccg aatctgaaga gatgttagct aaaatgctgg
    41221 acgctggcat gaacgttatg cgtctgaact tctctcatgg tgactatgca gaacacggtc
    41281 agcgcattca gaatctgcgc aacgtgatga gcaaaactgg taaaaccgcc gctatcctgc
    41341 ttgataccaa aggtccggaa atccgcacca tgaaactgga aggcggtaac gacgtttctc
    41401 tgaaagcggg tcagaccttt actttcacca ctgataaatc tgttatcggc aacagcgaaa
    41461 tggttgcggt aacgtatgaa ggtttcacta ctgacctgtc tgttggcaac accgtactgg
    41521 ttgacgatgg tctgatcggt atggaagtta ctgccattga aggtaacaaa gttatctgta
    41581 aagtgctgaa caacggcgac ctgggcgaaa acaaaggtgt gaacctgcct ggcgtttcca
    41641 ttgccctgcc agcactggct gaaaaagaca aacaggacct gatctttggt tgcgaacaag
    41701 gcgtagactt tgttgctgct tcctttattc gtaagcgttc tgacgttatc gaaatccgtg
    41761 agcacctgaa agcgcacggc ggcgaaaaca tccacatcat ctccaaaatc gaaaaccagg
    41821 aaggcctcaa caacttcgac gaaatcctcg aagcctctga cggcatcatg gttgcgcgtg
    41881 gcgacctggg tgtagaaatt ccggtagaag aagttatctt cgcccagaag atgatgatcg
    41941 aaaaatgtat ccgtgcacgt aaagtcgtta tcactgcgac ccagatgctg gattccatga
    42001 tcaaaaaccc acgcccgact cgcgcagaag ccggtgacgt tgcaaacgcc atcctcgacg
    42061 gtactgacgc agtgatgctg tctggtgaat ccgcaaaagg taaatacccg ctggaagcgg
    42121 tttctatcat ggcgaccatc tgcgaacgta ccgaccgcgt gatgaacagc cgtctcgagt
    42181 tcaacaatga caaccgtaaa ctgcgcatta ccgaagcggt atgccgtggt gccgttgaaa
    42241 ctgctgaaaa actggatgct ccgctgatcg tggttgctac ccagggcggt aaatctgctc
    42301 gcgcagtacg taaatacttc ccggatgcca ccatactggc actgaccacc aacgaaaaaa
    42361 cggctcatca gttggtactg agcaaaggcg ttgtgccgca gctggttaaa gagatcactt
    42421 ctactgatga tttctaccgt ctgggtaaag aactggctct gcagagcggt ctggcacaca
    42481 aaggtgacgt cgtagttatg gtttctggtg cactggtacc gagcggcact actaacaccg
    42541 catctgttca cgtcctgtaa tattgctttt gtgaattaat ttgtatatcg aagcgccctg
    42601 atgggcgctt ttttatttaa tcgataacca gaagcaataa aaaatcaaat cggatttcac
    42661 tatataatct cactttatct aagatgaatc cgatggaagc atcctgtttt ctctcagttt
    42721 ttttatctaa aacccagcgt tcgatgcttc tttgagcgaa cgatcaaaaa taagtgcctt
    42781 cccatcaaaa aaatattctc aacataaaaa actttgtgta atacttgtaa cgctacatgg
    42841 agattaactc aatctagagg gtattaataa tgaaagctac taaactggta ctgggcgcgg
    42901 taatcctggg ttctactctg ctggcaggtt gctccagcaa cgctaaaatc gatcagctgt
    42961 cttctgacgt tcagactctg aacgctaaag ttgaccagct gagcaacgac gtgaacgcaa
    43021 tgcgttccga cgttcaggct gctaaagatg acgcagctcg tgctaaccag cgtctggaca
    43081 acatggctac taaataccgc aagtaatagt acctgtgaag tgaaaaatgg cgcacattgt
    43141 gcgccatttt tttgtctgct atttaccact actgcgtcgc gcgtaacata ttcccttgct
    43201 ctggttcacc attctgcgct gactctactg aaggcgcatt gctggctgcg ggagttgctc
    43261 cactgctcac cgaaaccgga taccctgccc gacgatacaa cgctttatcg actaacttct
    43321 gatctacagc cttattgtct ttaaattgcg taaagcctgc tggcagtgtg tatggcattg
    43381 tctgaacgtt ttgctgttct tctgctgata gtggtcgatg tacttcaaca taacgcatcc
    43441 cgttaggttc cacggaatat ttcaccggtt cgttgatcac tttcaccggc gttcccgtcc
    43501 gcacgctgga gaacaaggct ttaatatccg gtgcattcat gcgaatacac cctgaactga
    43561 cgcgcaaacc aacgctgtcc ggcgcactgg tgccatgaat gaggtattcg ccattaccat
    43621 gcgcgaggcg cagtgcgtaa cgtcctagcg ggttatttgg tccggcagga acgactggcg
    43681 gtaatttaat gccacgctcc agcgaacgct gacgaatgcc tgccgtaggc gtccaggttg
    43741 ggttagggat tttctgccca acacgcgttt ccatcaccgg cgtttccagc ccctgcaatc
    43801 caatacctat tggataaacc tgcacaatat tttctcccgg cggataataa taaaggcgca
    43861 gctctgcaag gttaacgata atcccctgac gcggtgcatc aggtaataac agttgtgaag
    43921 gaatagttat cgtcgtacca ggttttggca ccgggacgat agtgttattg gcttcaagga
    43981 tcaacattgc cgcagtatca aaacgtcggg caatagcctg aaggttttta tccccttctt
    44041 gcaccgtata cgtttgattt tgcccaacca gtcggcttcc ggttggtggt agcggataat
    44101 caaccgccca ggcagcctgg atggcgctaa aagcgccgat aagcgtgagt gtaagcaaag
    44161 acgcgcgttt cattgtaaac ctcctgtatt tgccggagac tcacgctgaa acgtcggatg
    44221 gcgcttatgt tcacctgaaa ccaaaacact cctgtgcagg tcagtgtaaa cattgaccat
    44281 ccggcaatgt gagccaaccg gatgaaagct gtccctttag tttagctaag tgcagcggct
    44341 ttggcgcgaa ttgcgcgaat catcgcttcc agaccttgtg aacgagatgg ggtgagatgt
    44401 tgggtgagtg ccattttttc aaaccacgga cgcacatcga aactgacaat atcctgcggc
    44461 gtcatctgat cgtagagaat aaagactacc gcaataagcc ctttcacaat cgccgcatcg
    44521 ctgtcgccct gcaattcaat aattccgtgc gcattctggc gcatgacaat ccacacctga
    44581 ctctggcagc cctgaatgct attttgtggg cttcggtctt cgtcgcgtaa ttcagtcaga
    44641 cgctggccca gctcaataat gtagagatat ttctcttccc agttggcgca gcgtaaaaaa
    44701 ttacgcagca atttttcttt atccggcaat aaagccatag tgcctccctg ttatcccagc
    44761 aaacggtgaa tacgttgcag gccggtcacc agacgatcca cttcttcatg ggtgttatac
    44821 atggccagcg acgcccgaca catcgcaggg acgttgtaat aggccattaa tggcatagcg
    44881 cagtgatgtc cggtacgcac agcaatgccg taattatcga gaaaactgcc aacatcatag
    44941 gcgtggtgtt taccgagatt aaaagcaata acgccaagcc tgttttgtgg gccatagaga
    45001 gtgagatccg gtacagattc cagctgtgat agcgcataat gcatcagatt ctgttcatac
    45061 tcggctatgt tattaagccc cagcgccgaa acatactcca gcgccgcgcc aagaccaata
    45121 atgcccccgg tattgggtgt accggcttca aaccgccatg gtgctttggt ccaggtagtg
    45181 ccttcactca ggctgacggt ggcgatcata gaaccgcccc cttcccacgg cggcatctcc
    45241 tgcaacaagg cttctttcac ataaagaatg ccaattccgg tggggccata cagtttatgc
    45301 ccggagaaca cgtaaaagtc gcaatccagc gcctgaacat ccaccggatg atgcatcacc
    45361 gcctgagcgc catccaccag cacttttgcg ccatgctggt gcgcaagcgt gattatttcc
    45421 gccagcggat tttctgttcc cagcacgttg gagacatgag taattgccag caggcgagtt
    45481 ttctcatcaa acagcgtagg cagcgtctcc agttgcagcg taccgtcggg gttgagaggg
    45541 atcacacgca gctctgcgcc aacgcgtgcg caaagcatct gccagggaac aatgttagcg
    45601 tggtgctcca tctgactgat gatgatgtta tcgcccgccc gcacgttgct gttgccccaa
    45661 ctattggcga ccagattgat cccttccgtc gtgccgcgga cgaacaccag ctcttccgcc
    45721 gaacgggcat taataaacag cgatgcccgc ttgcgcacgt tctccatttt ctcggtcgcc
    45781 tgggcgctta aggtatgaat accgcgatgc accgccgcgt agccgtgacg ataaaactca
    45841 gcctcggcgt caatcacctg gcttggtttc tgcgcactgg cggcgctgtc gagataagcc
    45901 agcggcaaac cgttcacctc acgagaaagc accggaaagt cggcccgcac tttgtcgacg
    45961 gaaaaagtca tcttgcacct cctggcagcc gttgaccgat tcgggccagc acctgctgtt
    46021 taagcccctc atcacgcagt gcttccgtca gttcggcagc aaaggcgtaa atgatcatct
    46081 gctgggcatc ctgctgattg atcccgcgcg agcgcagata gaacatctgt tcatcatcaa
    46141 tacgccccac cgtcgcgccg tggctgcatt tcacatcatc tgcatagatt tccagctgcg
    46201 gttttgtatc cacttccgcc attttgccca tcagcagatt attgttggtc atctgaccat
    46261 ccgttttgat ggcgtgctgc gcgacgttga tcaaaccgtt aaataccgcg cggcctttgt
    46321 cgctgacgat agttttgtgc aactgtcggc tgttacaaaa acctttattg tgttccagcc
    46381 aggtacgggt atcacacacc tcgtttttca ccggcatcgc caggctattg atccgcagcg
    46441 tgctgttttc gccattgagt tgcgtactgg tgttgtgtcg taacactgcg ccacccagca
    46501 ggaaactatg gctaaatgcg gtggcatcct cagccagcaa caaatcgtta tgagcaaagt
    46561 ggtgactgag cgggttttca aacgccagct tgatatgctg caagtgggcg ttcgctgcga
    46621 cattgatagt gaaccgtgcc ccggtaaaat gacgagcatc attaaggctg acaaaatgtt
    46681 cgatcaccgt tgcttcggca ccttccgcca gatccagatg atgccggtaa tgggcagtgt
    46741 tcacctcttc acctgccacg ccctgggtga tatgcattaa cagcaatggc tttgccggcc
    46801 gttgaccgcg cttcacggcg atatgcgtca cgctttgtgc caggctttcc gtcaaatgca
    46861 gaaacacttc cgcctgaata gcgtcgggta acccctgacg gtcgtcgtta atgctcactt
    46921 catatccgct gccttcaatt gcatcgctca gcgcgggcac gtaacgtcca tcgacaaaca
    46981 ccagccgcac ggcgtctaac gttaacgcta aggcatcacg ctgctgtggg gatatatctc
    47041 ccgcaatgct gacaaactga ctattggtca gcccttccag cggcgtatat ttccagtttt
    47101 catgtttacg tgtcggcagt ccggtacgca gcaattgttg taaatgctgc tgtgcttgcg
    47161 gtgagcgttt tgtcccttca gcttcaaaca agtgatgcca ctgttgcagc gcattactgc
    47221 tgttcggtaa gccagccata accctgctcc tccagttgtt tgaccaacgt gaaatcgccg
    47281 gatttcacaa ttcgtccctg atatagcaca tggacgtaat caggcttgat gtagtcgaga
    47341 atgcgttggt agtgcgtaac aatgatgaat gagcgcttgc cgtcacgcag cgagttcacg
    47401 ccatcggcaa ccacttttaa tgcgtcaata tccagcccgg agtccgattc atcaagaatg
    47461 cataactccg gttccagcac cgccatttgc aaaatatcgt tgcgcttttt ctcgccgccg
    47521 gaaaaaccaa cgtttaccga acgggttaat aaatcttccg gcatcttcag gagagcgatt
    47581 ttctcttcca tcaaatcctg aaaatcaaag cggtcgagcg tttcctggcc gcgatagctg
    47641 cgcaccgcat taagtgccgt ttgcaggaaa aactggttac tgacgccggg aatctccacc
    47701 ggatactgga aggccataaa gatgccttcg cctgcacgat cttccggcga cagcgcaagc
    47761 aaatctttgc ctttgaactc aaccgtgccg cccgtcactt cataatcttc tcgcccggca
    47821 agcgttgccg ataaggtact tttgcccgaa ccgtttggcc ccataatggc gtgaacttcg
    47881 ccgggacgaa cgtcgaggct taatccacgc aggatagctt tatcttccac gctgacgtgt
    47941 aaatctttaa tacttaacat gtttattcct tatccgacgc tgtgttcaag actgatggcg
    48001 aggagttttt gtgcttcaac ggcaaattcc agcggcagtt ctgagaacac gtctttgcag
    48061 aaaccgttaa caatcatcga gatggcgtct tcttcgctaa tcccgcgttg caggcagtaa
    48121 aacagttgat cttcaccaat acgtgatgtc gttgcctcgt gctccagttg tgcgctgtta
    48181 ttgcgacatt cgacatacgg gaaggtatgc gccccacaat tagcgccaat cagcattgag
    48241 tcgcactgag tgaaattgcg cgcattggtt gccgtcggca tgattttcac taagccgcga
    48301 taactgttct gactatgtcc ggcagagatc cctttcgaga taatggtcga tttggtgttt
    48361 ttaccgatgt ggatcatctt ggtgccggta tccgcttgct gatgaccgct ggtcagcgcc
    48421 actgagtaaa actcaccaat ggagttatcg ccgcgcaaaa tgcagctggg atatttccac
    48481 gtaatcgctg acccggtttc tgattgcgtc catgacattt tgctgttttc gccttcgcac
    48541 aaagcacgct tggtgacgaa attgagaata ccgccggtgt tgttatcgcc agggaaccag
    48601 ttttgtaccg tggaatattt cacctcggcg tttttatgga tgatgacttc caccaccgcc
    48661 gcgtgtaact gatagctgtc acgcaccgga gcggaacagc cttcaatgta gctgacgtag
    48721 ctgtcttcgt cggccaccag aatggtgcgc tcaaactgcc cggttttttc cgcgttaatg
    48781 cgaaaatagg tggaaagttc catcgggcag cgcacgcctt taggcacata aataaacgta
    48841 ccgtcagagg ctaccgccgc attaagcgcg gcaaagaagt tgtcatttcc cggcaccaca
    48901 gtgccgagat atttacgcac cagttccgga tgatcgtgga tcgcctcacc aaaggaacag
    48961 aaaataattc cctgctccgc cagtttttcg cgataagtag tggcaaccga aacagagtcg
    49021 aaaatggcat ccaccgccac ctctttgcct tcccgcacgg gaacgcccaa ctgctcaaac
    49081 gccgcctcca cctctttact taaaaaggcg ttcgcgccag tttgctgcac cgcgccaggt
    49141 tcagacgcgc aagtgtcgtc acaattaccg cacgatggtg ctgagtagta actgtaatcc
    49201 tgataattca gcttgtcgta gtgcgctttc aaccagtgcg gttcttccat ctccagccat
    49261 gcgcgatagg cgtttagacg aaactccagc atccactccg gctcattacg cttcgccgaa
    49321 attgcgcgca ccacctcttc gtttatcccc tttgccagct catcggtggc taactgggtg
    49381 aagaatcctt ctttataatt cagcgggccg ccggtccagg ttttgacatc gtcagttgct
    49441 tcagtattac gagacatagt accgcctata ccccaaagct ttcgccacag ccacattcat
    49501 tctgggcttt agggttgtga aatttgaata tctgattaag tccttcacga acgaaatcga
    49561 cttccgtgcc atcaataaac ggcatcgctt gcagcgggac aaacagcttc gcgccgtcgt
    49621 gttcaaacag cagatcgtct ttgtccggct cgctaacact gtcgagcaca tagccaaagc
    49681 ccgcgcagcc cgtttgcttc acgcctaagc gcacgccgac catacccggc tgctttgcca
    49741 ccagctcacg gatgtgtatc gccgctgcgg gtgtcagcgt taagccttgc caggcgaaat
    49801 cttgtgggtt aaaggttcct gaatgcatgt ccatcgattt acctcacttc atcgctttca
    49861 gcgtataaca gcatgctagt gataatgatt atcagttcaa cccagcaaac gcaggggctt
    49921 taccgtaaaa catgcttttt gcctgctttt aataagcata gaccctgatg tgtgggttaa
    49981 caggcacgct aagaattagg tatctcattg ttagataatg gttattatct aaggagcatt
    50041 aaggctgtga tgaaaaagaa aaagttgtat tgaaaatgac tatttaagag ataggtaaaa
    50101 aagtgcagcg ttcagaaata agaaaaccct taagtctgtg cgacacaggc ttaagggttt
    50161 ctaccccatc cggcgcttat ctccggcact ctcagtggct tagctcttga aggggcgata
    50221 agaataatct cataaagcta acccgccgtt ttaacacaaa ctgcgattag tattattttt
    50281 gaacaatatc aggcggtaga taagcagtat taagaaggtc atcgaacctg gacggaggtt
    50341 aatccaggtc gatttggcga acttgcggca ttaagtcagg atcaatgcac gcccagacgc
    50401 caggcaaagt agatttcttc ttttaattca gtagaagaga gagtaagcag gtcagcaaat
    50461 tcaagttcta gttgtttcag acgtttgaga tattgggcag gtgaaagatt gctctggtca
    50521 cggcgtaaaa attcaatggc cagctgggtg ggatcaagtt gagtagacat agcatcctcg
    50581 cttttagaca agacgtgcac agtataccac cgtttactgt gcagataatg accaaaagca
    50641 atatgcgtca cacttttctg gtgacaacgt cacaaaatgg cggtcgtcaa tcgtgacgaa
    50701 cagcacaaac gccctttctc atcgaagatt tcaatctgcc agacctggtg acgcgaaccg
    50761 agatgcaacg gtttacatat gccgcgcacc cgcccttctc gtgccgagcg gacgtgatta
    50821 gcattgattt ccagaccaac cactttttgc tcaccttcgg tacataaata accggcaacg
    50881 gaaccgatac tttcggccag taccacggat gctcctccat gcagcaaccc gaaaggctgc
    50941 tttgtccgcg agtctactgg cattgtcgct tcaagggtgt catcaccaat atgttcaaag
    51001 cgaatatcca ggaaccccac catgtttcct tcacccatag cattcagtgc ttccagggtg
    51061 attttccgtt tccatatcat ttaataatct ccagtaaagc ctgcacagga tggcgtaccc
    51121 ccgtgccttc aacccgtttt acctggctac ggcaggaata tccggtcgcc agacagcggt
    51181 tacgcggcag tcgctgcata gcctgatgcc aggataactc atagatcccg agcgaatttt
    51241 catggttttt cgcttcatgt ccgtaagtcc ctgccatgcc gcagcaaccc acgctgacat
    51301 tttccagttt cgcgccaaaa cgggcaaata tcgcggccca ttgtgctggc gcgcccggca
    51361 aagcggtaac ctcggtacag tgaccaaaga aataccatga ttcaccgctg actgtagcca
    51421 ctggatgtga ctcaagtgcg cttgccagcc attcattcgc aagtaagacg ttaaactcgc
    51481 cacgttcctc gcccagcgcc agtttatatt catcgcgata acaaagtacc agcgccggat
    51541 cgacgcccac cattggcatc ccaagcttag ccatacggtt gaggaaatcc gccgtctttt
    51601 tcgccgtctt cgcaaaacga ttaagaaagc ctttgatatg ctgggctttg ccatttggcg
    51661 aaaatggcag taacacaggc tggaaaccta atttttcaac cagacggaca aaatccgcca
    51721 ccacttgcgc atcgtaatag ctggtaaagg ggtcctgcac caccaacacc gtgcgcgctt
    51781 tctgctctgc attgagagct tcaagctgtt ccagcgtcat gtttgccgag cgatgcccca
    51841 ccatttgttg ttgtagcgag gggaccgaca gcagtggcaa atcaaccatg ccgatatgtt
    51901 tttccgagag tttgcgcacc agcggctggt taatgaagaa gttaaaggtt ttcggcgcgc
    51961 gtgccatcag cggtgcgtag ctctcgaccg tagcgacgag gtggtcgcgc agcgggcgta
    52021 aataacgggt gtgatagagc tgcaggaaac gagagcgaaa ctccggcaca tcaattttga
    52081 tggggcactg ggtcgaacac gctttacagg ccagacagcc cgacatcgcc tctttgactt
    52141 cgtgtgagaa gtcatattcg cctttattcg catgccagct attgcgcgtg cgggcaatta
    52201 acgtccgtaa actgacgccg gattcaggca gttcttgttc cagtttgagt ggatcaacgc
    52261 cgcgatccgc caacaaacgc agccattcac gcaccagcgt tgcgcgccct ttcggtgaat
    52321 gaatccggtt ctgggtgatc ttcatcgacg gacacatcgg actacgggca tcaaagttga
    52381 agcataaacc gttgccgtta cactccatcg caccgcgcca ctgctggcgt accgcaatgg
    52441 ggatctgccg atcgaatgta ccgcgcttca ccgcgtccac tttcatcatc ggcgcatcga
    52501 gaccttctgg cgggcaaatc ttccctgggt tgagtcggtt atgcgggtca aatgccgctt
    52561 tcactttgcg cagttctgca aaaagttcct caccgaaaaa cgccgggctg tattcagcgc
    52621 gaaaaccttt gccgtgctcg ccccacaaca aaccaccgta tttcgcagtc agcgccacca
    52681 cgtcatcaga gatttgcttc atcaaaatct cttgttgcgg atcgcacata tccagtgccg
    52741 gacggacgtg caaaacacct gcgtcgacgt gaccgaacat accgtagctt aagccgtggc
    52801 tgtcgagcag cgcgcgaaat tcagcaatat aatcggccag atgttccggc ggtacgcagg
    52861 tatcctcagc aaacggaatt ggcttagcgg cacctttggc attgccaagc agaccaacgg
    52921 cttttttgcg cattgcatag atacgttcaa cccccgccag ctcgcggcac acctgccagc
    52981 caatcacacc tgcctgttgg ctggcaatca gctcatcaag ccgcacacag agtgcattaa
    53041 ctcgctcgtc aatcagcgcc tcatcatcac cagcaaattc cacaatgttc agcccgagca
    53101 tctctttgtc aggcacatcg gtaatcaact cgctgacgga atgccagaca atatcttccc
    53161 gcgccagatt cagcactttt gagtccaccg tctctaccga aagcgcccgc gcctcaacca
    53221 taaacggcgc gttacgcagc gcggagtcaa aagagtcata tttgacgttc accagacggc
    53281 gcactttagg caagggcgta atatccagcc gcgcttcggt aataaaggcc agcgtccctt
    53341 ctgaacccgt cagaatgcgc gtcaggtcga actcggtcat ctcatcgtta aagacatgac
    53401 gcagatcgta accggtaaga aagcggttaa gtttggggaa gttgtcgatg attaactggc
    53461 gttgcagacg gcaacgttga taaaccgtgt tataaattcg cccgattgtg gtattggttt
    53521 tacccagcgt ttccgccagt tcgacgggta aaggttgcgt atcgagaata tcgcccccca
    53581 acaacaccgc gcgtacgcca agtacgtgat ctgacgtttt gccatagacc agcgatccct
    53641 gaccggatgc atcggtattg atcatcccgc cgagcgttgc ccggttgctg gtcgaaagtt
    53701 ctggtgcaaa aaagtagccg aacggtttca ggtactgatt gagttgatct tttatcaccc
    53761 cggcctcaac gcgcacccag ccctcttcag ggttaatttc gatgatgcgg ttcatatggc
    53821 gggacatatc aacaataatc ccctggttga gcgcctgacc gttagtgccg gtgccgccgc
    53881 cgcggggggt aaagatcagc gatgaatagc gttcctgcgc ggcaagacgg gcgatcagcg
    53941 ccacatctgc ggttgaacgc ggaaatacca ccgcatcggg gagaagttgg taaatactgt
    54001 tgtcggtcga cattgtcaga cgatcggcat aacttgtcgc cgtatcgccg gtaaaacctt
    54061 gttgctccag ctcttgcaaa aaattaagca ccagttgaac gacgccgggt gcctgggaaa
    54121 tctgtggaat cattatattg accctttcct gcggtctgtg atgtaggtcg atacactatt
    54181 ctttcaggcg gctgcaatag cgcactgaaa ggtgatgttt atttactcta tggatttcga
    54241 gttgcaggaa ggcggcaagc gagtgaactc caggagctta caatagtaag tgactggggt
    54301 gaacgaacgt agccgcagcg catgcaactt gaaatacgac gagtaaatcg tttgcgtgtt
    54361 gcctgagttg ttgtaccaca tttttttcta acacgcccat cagaattaag ggcagaatcg
    54421 gcctgttaaa aaccgctgaa attgctcatc attatgcagg tgagtttcgc gtgttcacgt
    54481 cgcgtcgacg atttgacgca caaaaaaggt gaaaagtagt tatggtaaat gttcgtcagc
    54541 ccagggatgt cgcacaaatt ctgctttcgg tgctgttttt agccatcatg attgtggcat
    54601 gtctgtggat tgttcaaccc tttattctcg gctttgcatg ggccggtacg gtggttatcg
    54661 ccacctggcc ggtattgtta cgtttgcaaa agatcatgtt tggccgccgc tctctcgccg
    54721 ttctggtgat gacgctgtta ttagtgatgg tgtttatcat ccctattgct ttgctggtta
    54781 acagtatcgt cgacggcagc ggcccgctaa ttaaagccat ttccagcggc gacatgacgt
    54841 tacccgatct ggcgtggctt aataccattc cggtgattgg cgcgaagctg tatgcaggct
    54901 ggcacaactt gctggatatg gggggtacgg cgatcatggc gaaagtccgc ccttatattg
    54961 gcaccaccac cacctggttc gttgggcagg cggcgcatat cgggcgcttt atggtgcatt
    55021 gtgcgctgat gcttctcttc agtgccctgc tgtactggcg cggtgaacag gtggcacaag
    55081 gcattcgcca ttttgcaacc cgtctggcag gcgttcgcgg agatgccgcc gtgctgctgg
    55141 cggcacaggc tatccgcgcg gtggcgctgg gtgtggtggt gacggcgtta gtacaggcag
    55201 tgcttggcag tatcggcctc gccgtatccg gcgtacctta tgcaacgttg ctaacggtgt
    55261 taatgatcct ctcctgcctt gtccagcttg gcccgttgcc ggtactgatt ccggcgatta
    55321 tctggctcta ctggaccggc gataccacct ggggaacggt attgttagtg tggagcggtg
    55381 tggttggcac gctggataac gtcatccgcc caatgttaat tcgcatgggt gccgatttac
    55441 cgctgatcct gatcctctct ggcgttattg gtggtttgat tgctttcggg atgatcggtc
    55501 tgtttattgg tccggttctg ttagccgttt cctggcgtct gtttgccgcg tgggtggaag
    55561 aagtcccgcc gccgacggac caaccggaag aaattctcga agaacttggc gaaatcgaga
    55621 aaccgaataa gtaatttctc atcaggcggc tctgccgcct gattgttaat caccgctaat
    55681 tgatatttgc ttgctcttcc ccatattttc ctgcttaccg ccattcagct
//
LOCUS       DAQSWQ010000016        54950 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.016, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000016 DAQSWQ010000000
VERSION     DAQSWQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.016"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="R8G18_000990"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="R8G18_000990"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEE9794771.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="R8G18_000991"
     CDS             complement(1672..2730)
                     /locus_tag="R8G18_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEE9794772.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="R8G18_000992"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="R8G18_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="R8G18_000993"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="R8G18_000993"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HEE9794773.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="R8G18_000994"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="R8G18_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HEE9794774.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="R8G18_000995"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="R8G18_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HEE9794775.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="R8G18_000996"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="R8G18_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HEE9794776.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="R8G18_000997"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="R8G18_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HEE9794777.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="R8G18_000998"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="R8G18_000998"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HEE9794778.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="R8G18_000999"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="R8G18_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HEE9794779.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="R8G18_001000"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="R8G18_001000"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEE9794780.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="R8G18_001001"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="R8G18_001001"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEE9794781.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="R8G18_001002"
     CDS             complement(17699..18322)
                     /locus_tag="R8G18_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HEE9794782.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="R8G18_001003"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="R8G18_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HEE9794783.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="R8G18_001004"
     CDS             complement(18971..19213)
                     /locus_tag="R8G18_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HEE9794784.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="R8G18_001005"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="R8G18_001005"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HEE9794785.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="R8G18_001006"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="R8G18_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HEE9794786.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="R8G18_001007"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="R8G18_001007"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEE9794787.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22593..24290
                     /gene="ypdA"
                     /locus_tag="R8G18_001008"
     CDS             22593..24290
                     /gene="ypdA"
                     /locus_tag="R8G18_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HEE9794788.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24305..25039
                     /gene="ypdB"
                     /locus_tag="R8G18_001009"
     CDS             24305..25039
                     /gene="ypdB"
                     /locus_tag="R8G18_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HEE9794789.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25052..25909
                     /locus_tag="R8G18_001010"
     CDS             25052..25909
                     /locus_tag="R8G18_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9794790.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="R8G18_001011"
                     /pseudo
     CDS             complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="R8G18_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="R8G18_001012"
     CDS             complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="R8G18_001012"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEE9794791.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="R8G18_001013"
                     /pseudo
     CDS             complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="R8G18_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30569..31816)
                     /locus_tag="R8G18_001014"
     CDS             complement(30569..31816)
                     /locus_tag="R8G18_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEE9794792.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31838..32164)
                     /locus_tag="R8G18_001015"
     CDS             complement(31838..32164)
                     /locus_tag="R8G18_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HEE9794793.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32384..33349)
                     /gene="glk"
                     /locus_tag="R8G18_001016"
     CDS             complement(32384..33349)
                     /gene="glk"
                     /locus_tag="R8G18_001016"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEE9794794.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            33553..34809
                     /locus_tag="R8G18_001017"
     CDS             33553..34809
                     /locus_tag="R8G18_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HEE9794795.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34924..35250
                     /locus_tag="R8G18_001018"
     CDS             34924..35250
                     /locus_tag="R8G18_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HEE9794796.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35391..36629)
                     /locus_tag="R8G18_001019"
     CDS             complement(35391..36629)
                     /locus_tag="R8G18_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEE9794797.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36965..38167
                     /gene="nupC"
                     /locus_tag="R8G18_001020"
     CDS             36965..38167
                     /gene="nupC"
                     /locus_tag="R8G18_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HEE9794798.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38217..40405)
                     /locus_tag="R8G18_001021"
                     /pseudo
     CDS             complement(38217..40405)
                     /locus_tag="R8G18_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40614..40689)
                     /locus_tag="R8G18_001022"
     tRNA            complement(40614..40689)
                     /locus_tag="R8G18_001022"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40654..40656),aa:Ala,seq:ggc)
     gene            complement(40729..40804)
                     /locus_tag="R8G18_001023"
     tRNA            complement(40729..40804)
                     /locus_tag="R8G18_001023"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40769..40771),aa:Ala,seq:ggc)
     gene            41025..41384
                     /locus_tag="R8G18_001024"
     CDS             41025..41384
                     /locus_tag="R8G18_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HEE9794799.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41386..41778
                     /locus_tag="R8G18_001025"
     CDS             41386..41778
                     /locus_tag="R8G18_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HEE9794800.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="R8G18_001026"
     CDS             complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="R8G18_001026"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEE9794801.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGRSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43504..43579
                     /locus_tag="R8G18_001027"
     tRNA            43504..43579
                     /locus_tag="R8G18_001027"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43537..43539,aa:Val,seq:tac)
     gene            43624..43699
                     /locus_tag="R8G18_001028"
     tRNA            43624..43699
                     /locus_tag="R8G18_001028"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43657..43659,aa:Val,seq:tac)
     gene            43746..43821
                     /locus_tag="R8G18_001029"
     tRNA            43746..43821
                     /locus_tag="R8G18_001029"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43779..43781,aa:Val,seq:tac)
     gene            43826..43901
                     /locus_tag="R8G18_001030"
     tRNA            43826..43901
                     /locus_tag="R8G18_001030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43859..43861,aa:Lys,seq:ttt)
     gene            43932..44057
                     /locus_tag="R8G18_001031"
     ncRNA           43932..44057
                     /ncRNA_class="other"
                     /locus_tag="R8G18_001031"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            44177..44518
                     /locus_tag="R8G18_001032"
     CDS             44177..44518
                     /locus_tag="R8G18_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HEE9794802.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(44509..45435)
                     /locus_tag="R8G18_001033"
     CDS             complement(44509..45435)
                     /locus_tag="R8G18_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9794803.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            45525..46523
                     /locus_tag="R8G18_001034"
     CDS             45525..46523
                     /locus_tag="R8G18_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEE9794804.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46520..46738)
                     /locus_tag="R8G18_001035"
     CDS             complement(46520..46738)
                     /locus_tag="R8G18_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HEE9794805.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46740..48755)
                     /gene="ligA"
                     /locus_tag="R8G18_001036"
     CDS             complement(46740..48755)
                     /gene="ligA"
                     /locus_tag="R8G18_001036"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEE9794806.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48826..49812)
                     /gene="zipA"
                     /locus_tag="R8G18_001037"
     CDS             complement(48826..49812)
                     /gene="zipA"
                     /locus_tag="R8G18_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEE9794807.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            50042..50803
                     /gene="cysZ"
                     /locus_tag="R8G18_001038"
     CDS             50042..50803
                     /gene="cysZ"
                     /locus_tag="R8G18_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HEE9794808.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50988..51959
                     /gene="cysK"
                     /locus_tag="R8G18_001039"
     CDS             50988..51959
                     /gene="cysK"
                     /locus_tag="R8G18_001039"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEE9794809.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52343..52600
                     /gene="ptsH"
                     /locus_tag="R8G18_001040"
     CDS             52343..52600
                     /gene="ptsH"
                     /locus_tag="R8G18_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HEE9794810.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52645..54372
                     /gene="ptsI"
                     /locus_tag="R8G18_001041"
     CDS             52645..54372
                     /gene="ptsI"
                     /locus_tag="R8G18_001041"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HEE9794811.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54413..54922
                     /gene="crr"
                     /locus_tag="R8G18_001042"
     CDS             54413..54922
                     /gene="crr"
                     /locus_tag="R8G18_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEE9794812.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT        14329 a        13803 c        12952 g        13866 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aataactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatcaatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
    22441 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
    22501 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22561 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22621 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22681 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22741 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22801 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
    22861 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22921 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22981 tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaga
    23041 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
    23101 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgcttc
    23161 tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc
    23221 tggatatcgc caacaaaacg ctaccgctgt ttcgccatgt caatagcgag tcattacgca
    23281 aggtctgcga aattatccgc gatgacattc acgccgatgc ggtggcgatt actaataccg
    23341 atcatgtgct ggcctatgtt ggcgtgggtg aacataacta tcagaatggc gatgacttca
    23401 ttagcccgac tacccgtcag gcgatgaatt acggaaaaat catcattaaa aacaatgatg
    23461 aagcccaccg cacaccagag attcattcca tgctggtgat cccattgtgg gagaaagggg
    23521 tcgtgaccgg aacgctgaaa atttactact gccacgcgca tcagatcacc tcgtcattac
    23581 aggaaatggc ggtcggtctg tcgcaaatca tctccacgca actggaggtt tcacgcgccg
    23641 agcagctacg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc
    23701 cccattttct gtttaacgct ctgaacgcta tttcatcgtc aatccgtctg aatccggata
    23761 ccgctcgcca gttgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag
    23821 acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg
    23881 agcaggcccg ctttggtgac aagctgacgg ttatctatga tattgatgaa gaggtgaatt
    23941 gctgcattcc cagcctgctg atccagccgt tggtggagaa cgccattgtc cacggtattc
    24001 agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcgga aatcgggtac
    24061 gcattgcggt gcgagatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag
    24121 cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat
    24181 tgtatggcga ggggctgcat atccgccgcc tggagccggg gacggaaatt gcgttttaca
    24241 ttcctaacca acgcacccca gtcgcctcac aggctacgtt attgctttga gccggagtga
    24301 tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct
    24361 aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt
    24421 gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct
    24481 ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt
    24541 catcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat
    24601 tctcaaaccg tatcaggagt cacgtattac cgggatgctg caaaaactgg aagcggcctg
    24661 gcaacaacag cagaccagca gtacgcctgc cgcgacggta acgcgtgaga atgacaccat
    24721 taatctggtg aaagatgagc gaataatcgt cacgccaatt aacgatatct attacgccga
    24781 agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa
    24841 cattaccgaa ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc atcgctcatt
    24901 ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg tttaataaca cctacattct
    24961 gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc agcaaagtga aagaatttcg
    25021 ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca
    25081 acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt
    25141 ctggtttgcc agccagcctg cctcgttacc ggtgggcagt ttatgtattg attttccccg
    25201 tctggatatc gtgctgcgcg gcgaatacgg caacctgctg gaagcaaagc agcagcgaat
    25261 ggtggaagga gaaatgctgt ttattccggc acgcgcggct aatttaccga tcaacaacaa
    25321 accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat cgttttacga
    25381 tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc cagcttccca gcctgcaacg
    25441 cggtgaaggt gaagcgatgc ttaccgccct cacccatctt agccgttcgc cgctggagca
    25501 gaatatcatt cagccactgg tgttaagttt gctgcatctt tgccgcaacg tggtgaatat
    25561 gccgccgggt aattcgcaac cgcgcggcga ttttctctat cacagcattt gtaactgggt
    25621 tcaggataat tatgcccagc cgctcacccg cgagagcgtg gcgcagtttt ttaatatcac
    25681 gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg atgcgtttta tcgagtatgt
    25741 gcgttgggtg cgaatggcga aggcgaggat gattttgcag aaatatcatc tgtcaattca
    25801 tgaagtggca cagcgttgcg gttttccgga tagcgactat ttttgtcgcg ttttccggcg
    25861 tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac gtcagaaggt
    25921 taattctgtt tccagcagcg tcaggatact ttgtgcatct tgcgcggcaa acaacgactg
    25981 gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca catgattcat
    26041 cccttcgtta gcacccagcg tcagcatgat caccagttca acttcgccca tttctgactg
    26101 ccagtcaacc ggtttcgcca gccgggcaat gctgatactg gaatgacgga tccactgaga
    26161 tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct cttcccgctg
    26221 ccagacatct tcttccagct caaacggatg ttcagtgcgc ccgttaacgc cgaggttgcc
    26281 gcacaggaac tggatcgcct gctctttatt gctaaaatcc tgatcaacaa agatattctc
    26341 cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag gcggtgagta acgcttcaat
    26401 ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt tcccgacacg cctcgctatc
    26461 cagttgacga agctggcttt tcaccgccgg aatacgcggg ctactcatac tcagctcatc
    26521 caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt caccgcaaat
    26581 gcctacccat ttgccccgct gatgcgcagt ggtaactatt tgctgcaaca tgcgcaggaa
    26641 cgatggcgta atcgggttat atagcggcga tacgcgcggg ttattacgat cgaccgcata
    26701 cagatactgg gtcatatcgt tggagccgat actgaagaaa tcgacctcat cggagaagtg
    26761 gtcgatgatg tagcacaccg acggcacttc caccatgatc ccaagcgtaa tcgtctctgc
    26821 atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc ctttcaccca
    26881 taagatctga tcgaggctgt gaaccatcgg gatcatcaac tgggcgttgc cgaaactggc
    26941 ggcgcgcaaa atggcccgca gttgagtgcg gaacaggcca gcaaattccg ggtaaatacg
    27001 taccgcgcga tagccgagga acgggttctc ttcctgggga atattcagat aaggaatgct
    27061 tttatcgccg ccgatgtcca tcgtgcggaa gataatcggc ttgtcgcccg ccgccagtag
    27121 cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcgctgtcgc gatccatata
    27181 gagcatttcg gtacggaaca gcccgacacc ttccgcgccg ttggcaaacg cgcctggcgc
    27241 ttccagagcg gtgccgatat tcgccgcaat atcaatacgc ttgttatcac gggaataggc
    27301 cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta tccgccagcg tctgcgcgac
    27361 ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc agcacgccgc actgggcgtc
    27421 aagcactgca ggttgcccgg cataacgggc aatcgcatcc agcggcaagc cactcagtac
    27481 cgggatcgcc gaggcacggg ccagaatcag tgtatgcgag gtgcgcccgg ttttctccag
    27541 aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac tggcttgggg ttaaatcttc
    27601 agccaccaga atggtcggtt tttccagcac cagattgttg cgcggcttca gttccggcta
    27661 ggtgatatgc agcaactgtt cgctgatatc gcgaatgtcg ctgacccgct cgcgcagata
    27721 atcgctggca gaagcagata gtttggcgca aacctgctcc atattgctga tgatcgccgc
    27781 ccccagtccc tgatgctgtt ctgtcatcag gcgacggata ttgcctgcaa attcatcatc
    27841 ctgaatcagc gacaaatgag cgctgaggat agttttgctt tcgccgtcac gctcacgcaa
    27901 ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg tggaatcttg
    27961 cgcactggcg gggattgccc gataactgtc gaggctgtcg ctttgtaaca gggtcagcgt
    28021 acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatccgggt taaggcggct
    28081 taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gtcggctgaa cgctgtcgct
    28141 gtcgataaag cgcaccagga tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct
    28201 gccgctaatg ttcaggctgc aactgtcatt aaataaggtg ccagttccaa tcaacgccag
    28261 cgagctttta gcatcggcct ttgcgttctg gcgatggttg ataaaggtga tttcgctttg
    28321 ccactggctg cactgttctt taagttccca cgccggacga gcatgtagac cgttaggcaa
    28381 aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag atcatctgaa
    28441 atccgtcagt tgaacaaccg tctcacgcgt aagacgttga ataagggcag ataaaagttg
    28501 ctccatctgc aaaatgtcgc ggcaatcggc aatcgatgcc gcgcaatgtc cgtggcgggt
    28561 ggcaggcccc attaccactg tgggtacgcc agtatcggtt aagtgcaccg ctccaccgtc
    28621 cgtgccgccg ttactgaaca tatccgcctg caacggaacg ccaatttctg ccgacacggt
    28681 ttcaatccac gcggtaagtt ttggcggcgc aatcagcgat ttatcgctta acaccagcat
    28741 cggcccgtta ccaatctgac gatggttagc cgcgccataa tcaaagtttt tcgcccagca
    28801 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtagcag tttgcccgcc
    28861 gcgtaatccc acctcttcgc tggaacttgc caccagccac acttccgcag gcagttcggc
    28921 gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ccgaggcggt catcaaaggc
    28981 tttccccatc acccgctggt gagggagaac ctgaaaagtt gtatcaaagg tgacgcgatc
    29041 gcctggacga attcccgcct gcatcacttc gtcatacgag cgcgcaccaa tgtccacgcg
    29101 catggcgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
    29161 ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgca cgttgccaac
    29221 cggcagcaca tcaattgccc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
    29281 catatgcgca cagatcatca ccttcggacc tgtcgattca ttgaggcgga tcagcaccga
    29341 tcccagacca tcaaatcgta cttctttttg caggcgatcc gcttcttcca gtaggatctg
    29401 ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga
    29461 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
    29521 gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg ccctggcaaa
    29581 taaatccccg gctccacggt cagtaacatg cctggctgta gcgtcgtggt atcccgcggt
    29641 gaaaagcgtg gatcttcgtg tacttcaata ccgatagcat gaccggtgtt gtgaccaaaa
    29701 tagtcgccaa atcctgcctc ggtaatcacc cggcgcgcgg cgtcgtcaac ctgttagcag
    29761 cgtacgccag ggcgaatcgc ggagattgcc gccagctgtg cttgcaggac aatctgatag
    29821 acgttaaaca gcgggtgaga ttcggcaatt accccttcgc cattcaccag caaggtgcgc
    29881 gtcatatcag agcagtaccc ctgatagagc gcaccaaaat caagagtgac aaactcgcct
    29941 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccacgcca gccgctggcg
    30001 acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    30061 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg
    30121 cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc
    30181 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc
    30241 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    30301 atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt caagcaaatg cagctggtag
    30361 ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt
    30421 tcacggctaa taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    30481 tgccgtgagg agagaagcac tgcatccagt tgttgcgcct taagccagtc gcgcagcgaa
    30541 gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat ttacctttac
    30601 ggaacatcat cagacgcagg aacaccacca tcagcgcagt tatgactgcc cccagcgcga
    30661 tccccgccat atagacgccc aggttagtga ccagcggcca tgcccagata gcggattccg
    30721 ggaaccattg cactgcgccc agccagacag cggcggttga gccgacaatc gcgccgacca
    30781 tatacgacgg aatggcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa
    30841 tccccataaa ggcaaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca
    30901 ggcgtttgcc ggttaaacga cggtcgataa tggtcgccag acccagacct atcggcggaa
    30961 taacgatagc gatagaacgt gcagtgaccg gtaatacgtg gtcagtggta aagctgaagg
    31021 caacaaaacc tgcggctttg ttgatcgggc caccaaggtc gattgcggtc gccgcggcga
    31081 tccccattgc gtacatcagc gcgcccttct cacctgcggc agtcagcacg gtacggatac
    31141 cgccgttgat ccagccgcca aagggggtaa tgacgtagta catcgccagc atgacaaaaa
    31201 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    31261 ggattttctg gttcatccac ttcacaaggt aacccgcgac gatagaaata atcagcgcac
    31321 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt
    31381 tcagcagttg cgtcggctgg gtagacatca agccgccgat aaaaccagcc gggaacgcca
    31441 gtttgccacc gatagagttc gccacaaaag cagcaaacat cggaatggca aaaccaaaca
    31501 acacgccgcc gaaggactgc gacagccagg caaatttcag taaggagagg tcgaaaccgg
    31561 agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc
    31621 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    31681 tgcgtgaaat ccctaacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg
    31741 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    31801 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    31861 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    31921 cataaacgtc gcgtgattcg aaacgctcat tatcttccgg ggtaactgcc acggagtgga
    31981 tgatgatggt tgcttcggcg atatcctgcg ccgtcaggcg attctggata ccgtccgcgc
    32041 cctgggtttc aattttcact tcataacctg cttctaccgc cgcttcttcc agcgcctgag
    32101 cggccataaa ggtgtgagcc aggcccattg ggcaggcaca taaggcaatt agtttcttac
    32161 tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa
    32221 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    32281 tgacgcaggt cggccttgtg agggggacac aaagaaaaat tgaattaact gtatgattta
    32341 aaagattatc gggagagtta cctcccgata taacagaaag gatttacaga atgtgcccta
    32401 aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg acgatgagat
    32461 acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga
    32521 aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgccagca atataaaccc
    32581 caccaaaggt tcccagcgtt aatgccagat tgccgccaaa acggcccata atgacgcaaa
    32641 acagcgacaa cgcacggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat
    32701 cttttggctt gagattttct ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat
    32761 tcaccagccc aggaccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca
    32821 atatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt
    32881 cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccctg
    32941 ttccggcacc gtaaaccgca ataggtttac cttcgaccgg ttctgcgcca ccaaactgaa
    33001 tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa
    33061 tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat
    33121 ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt
    33181 tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat
    33241 caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa
    33301 gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc
    33361 tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac
    33421 gccggaaagg caggctccct gtaaatatcg atctgggtca cacatttact ttatcgtttc
    33481 agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat
    33541 aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc
    33601 gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta
    33661 ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca ggattcacct
    33721 atctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc
    33781 agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accagttccg
    33841 ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc
    33901 ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct
    33961 cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc
    34021 ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc
    34081 agtagtgaag ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt
    34141 gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga
    34201 cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca
    34261 gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat
    34321 ccggtgctcg tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga
    34381 ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc
    34441 agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc
    34501 gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg
    34561 ttgatgctgc atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt
    34621 ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc
    34681 gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg
    34741 ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat
    34801 aatgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct
    34861 ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata
    34921 acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca
    34981 aatgcaggtg aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat
    35041 tacggtaatt actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat
    35101 gaatggcgca aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag
    35161 cgtaaagcat atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga
    35221 aaaggccgcg gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga
    35281 tgcgacgcta gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata
    35341 aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa ctacaatccc
    35401 agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag
    35461 cctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg
    35521 aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata
    35581 accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg
    35641 actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc
    35701 cccgccagtg tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag
    35761 accgttgccg cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg
    35821 gcaacaccag tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga
    35881 ttgacaaaac cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt
    35941 tgttgacgcg aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc
    36001 ggcataatcg tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa
    36061 ctcgggatca ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg
    36121 acaatgtaag ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagtggtttt
    36181 tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc
    36241 tgcaacaacg agacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc
    36301 aggtcggttg ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc
    36361 ggatagtgat cacgaatctg ctccgccaga ttttttccgg tggcaatccc tagtttggca
    36421 gagaggatct gaatcagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga
    36481 tagccgaagc tggcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca
    36541 atcgccgcaa tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg
    36601 ctgctactct caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg
    36661 tttcatgcta tgataaacga gaatgattat caaattcatt taaatggatt gtggtgattt
    36721 ctctgataga ccaggattat gactacgaaa agattgatga cttgaatgtt atggttatgt
    36781 ttaatgttag cacatttaca taactttcag cttccataca caacatagca gaaatgtatg
    36841 acagatcact atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt
    36901 tttccttaac catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc
    36961 aaatatggac cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact
    37021 gctggtaagc agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat
    37081 cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg
    37141 cttctccgaa atgttcgaaa aactgctcag atttgccaac gaagggacta acttcgtctt
    37201 tggtagcatg aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt
    37261 ctttatctct gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg
    37321 cgcaattggt ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc
    37381 cgtcagctcc ctgattctgg gtcagtctga aaactttatt gcctataaag atatcctcgg
    37441 caaaatctcc cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat
    37501 gtccatcgtt ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt
    37561 actgaacatg ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc
    37621 cagtgaagaa aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct
    37681 gggtgaatac attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg
    37741 ctttatcgcc ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg
    37801 ctacagcatc tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat
    37861 gggtgttcct tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc
    37921 caacgagttc gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc
    37981 tgaaggcatc atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat
    38041 cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct
    38101 gaaactggtt tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt
    38161 gctgtaaaac catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa
    38221 tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta
    38281 tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc
    38341 gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata
    38401 tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg
    38461 tagttggcat atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc
    38521 tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca
    38581 agaatgaccg cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgcccggca
    38641 atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg
    38701 tcgcaagggt gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc
    38761 gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg
    38821 tcatatttca gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc
    38881 tgggcgtaga gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc
    38941 acctgtttag tggtctgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga
    39001 tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt
    39061 tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg
    39121 ttccagtgaa tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt
    39181 tccggcccgc tcagcactaa cagcagttca ctacccggca attgatataa cttttcgttt
    39241 tcctgcatca acggcagcag cgtacggtaa attgatcgga tacagtgaac gcgcatcatt
    39301 aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaactcttgc
    39361 ccgcctcttg ctccggcgct tgttccaacg cacggaagtt gggcaggagc gttaacggat
    39421 cggtcagcgc ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca
    39481 ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc aagaataaat gccagcgaat
    39541 attcggtttc caccccttgc aaaaagttct ggttgtaatt cagaaggcaa agcgttgaaa
    39601 cagcccaggt gagatttaaa aacggatagc gaagcttacc gaccccgagg gtaaagatga
    39661 tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca
    39721 gaagtagaag cacgcttaac gctgccagcc agcttaaggt aaatgcgcgt ttctctttgc
    39781 ccaacgacgg cgcgatatcc ctgcgccaca atatctgcgc aaagtgggga cttacaatca
    39841 tgcgagtgag atagtagaaa agcatgttgt aaataagcac agcggtgaaa aggcttagca
    39901 aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc tttagcggga
    39961 aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc gtcaccaaac
    40021 caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc caacgcgtcc
    40081 ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg ctaccgagca
    40141 tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa aacatcccgc
    40201 ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc attacgcaca
    40261 gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc gaaataaaac
    40321 gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc ttgatatttt
    40381 ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg gttttcaggc
    40441 acacaaataa taagtttatt cgcatcgcga agcaaacagg gggccagcct gttgccgatt
    40501 tttcatccaa tgatgctttc accaggcggc actaacccgg acgtagactg tattacaaaa
    40561 gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa atttggtgga
    40621 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    40681 tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa
    40741 gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag
    40801 ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc gcccgaatgt
    40861 gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt
    40921 ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa agtggtgtta
    40981 taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc aaggagagga
    41041 tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt aataaatggg
    41101 tacgcaagga gggctggaca acgtcaccaa aacctggcgt ccagggtggc aaagcccgac
    41161 tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc
    41221 agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg gtgacactgg
    41281 cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg cgggaaggga
    41341 ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa aagattacgc
    41401 aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca aactgttaac
    41461 cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt
    41521 gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac gccagccttc
    41581 cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc tcctggagca
    41641 cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga cattgagcag
    41701 ggaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat
    41761 attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt ttatcttttt
    41821 acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt tgttgatacg
    41881 ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg gagaccgccc
    41941 cgccccggtt acggcgacac gcagcggcat accaacttta cccataccca cttccagctc
    42001 atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag taatcgcggc
    42061 cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat gttttttcgc
    42121 cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct gtgccatctc
    42181 tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct gcgggccgtt
    42241 acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa catactccgg
    42301 cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt tgaacgcact
    42361 ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac gagtgaagat
    42421 ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg cttctggcaa
    42481 ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg acagtttttt
    42541 accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc
    42601 cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg
    42661 ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg gggaaccatc
    42721 ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg gaccacggat
    42781 ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa cacacggttc
    42841 atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc
    42901 tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat agcatttata
    42961 agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa aacgtttggt
    43021 ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat ccataatggc
    43081 ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca gcacgaactc
    43141 accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg
    43201 cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt
    43261 ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat attctatcac
    43321 tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg ttttcgttga
    43381 caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttccga cgaacgaaca
    43441 atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcgg ctccacacag
    43501 cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    43561 atcccgtcat cacccaccaa ctactttatg tagtctccgc cgtgtagcaa gaaattgaga
    43621 agtgggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    43681 atcccgtcat cacccaccaa tttctcgcca gctaaatttc ttgtaaaaat gtgaagtacc
    43741 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    43801 cgatcccgtc atcacccacc acttcgggtc gttagctcag ttggtagagc agttgacttt
    43861 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa
    43921 aggcgctttt ttgctatctg cgatactcaa agattcgaac ctgcagcagg tttgagttga
    43981 gcgcagcgaa acaacggagc cgctcgcggc gacggcccga agggcgagcg aagcgagtca
    44041 tcctgcacga cccaccaatg taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat
    44101 ttgtaagatt gatttttctt ctcaagttcc ttgttcttct gccgatatta cctcgtacag
    44161 tgacgaggac tgaacaatgg ccactatctc ttcaacttca attccttcca tacaaacaca
    44221 gagtagtaac agggcatcac aaggtagcga tgttgcttca aaaatcgcgc ggataagtca
    44281 gcaaatcatc aaactcactc aacaaattaa agagattgta gacactagcg gcagtgctga
    44341 agacaaacaa aaacaggccg aattgattca acagcaaatc accctactgg aaacacaact
    44401 ggcgcagttg cagagacagc aggcagaaaa ggcgcaggag aaagagcagc gtttgtctct
    44461 gaatgtatcc cttctaaatc ctgttgaaaa tacgactcat attgatatct atatctgata
    44521 caacggagcc ccttcccaac cagccattag cgcattgccc tggtctcgta ccacgtccca
    44581 taacgcttca gcggcggtgg aaagtgaacg gttcttcctg cgcaccagca tcaactggcg
    44641 ttcaacaacc ggcgtaatgc gctttaccac cagtgggcta ccttcaggca atggtagcgc
    44701 cagtgcggga agaatactga tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg
    44761 atgtccaatc tcctgcacaa tattcgcctg aataccattg cgcgccagcg ctgcgtcaat
    44821 cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat ttcgctccct gtaatgcctg
    44881 ccagggtaca taatcttcca cggccaaagc gctatcgcga tggcaaagca gaaagaaagg
    44941 ctcggaaaga atcgcttcac attgcagatc gcccactggg cctggatcga tgacgatgca
    45001 aaaatcgaca tctccctggc gaatactttc catcacccac tgctgcggac gatcgtgcag
    45061 gacaaactga atatctggat agcggcgatg gctttcagca atgcattgcg gaataagatg
    45121 cgcggaaatg gtctggctgg cagcgacccg aacttttccg ctcagttgtt gccccatacg
    45181 cccggtatcg cgcaacgtgc tgttaagttc atccagcagt cgctcaagac gcaaagccaa
    45241 ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc
    45301 agtatgattt tccagctcct tcacactgtg acttactgcc gactggctca agccgatacg
    45361 ctctcctgca cgactaaaac ttttctcctg cgctactgtg acgaaaacct ttaattgttt
    45421 taaagaataa ttcatctgtt ttcttcatga atggatgcaa taaatcaatt ttatttctca
    45481 aactggaaga agcacaatag aaccatcgat catctggagt ctttatgaaa ctttttcgta
    45541 tcctcgatcc tttcacctta accctgatca cggtggtgtt gctggcctct ttctttccgg
    45601 ccagaggcga tttcgtcccc ttctttgaaa atctgaccac cgcagctatt gccctgctgt
    45661 tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc
    45721 gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt
    45781 ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta ctccggtttt ctctacttgt
    45841 gcattctccc ggctaccgtg cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg
    45901 tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct ggggattttc ctttcaccat
    45961 tgctagttgg tctggtgatg aatgttcacg gtgcaggggg cagccttgag caggtcggta
    46021 aaattatgct gcaactgctg ctgccgtttg tgttggggca tctttcccgg ccgtggattg
    46081 gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt
    46141 tggtggttta tacagcgttc agcgaagccg tcgttaatgg tatctggcat aaagttggct
    46201 ggggatcatt gctgtttatc gtggtggtca gctgcgttct tctggctatc gtgattgtag
    46261 ttaacgtctt tatggcacgc cgactgggct tcaataaggc agatgaaatt actatcgtct
    46321 tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca
    46381 catcggtgat cggtatgatg gtgctgcccc tgatgatttt ccatcagatc caattgatgg
    46441 tctgtgcggt gctggcgcgt cgatacaaac gccagaccga acagttacag gcgcagcagg
    46501 aaagcagcgc cgataaagct taaagcggac gcttcagggg ctggaccagt tgcgtcagcc
    46561 cctcggtttt aatcagcagc gtgatttgca ttagctcacc gagcttccct gccgggaact
    46621 catctttgcg ggcaaaccac agcagatact cctccggcag gtcgattaag cgacgccctt
    46681 tgtatttgcc aaacggcatt atcgtattgg ctatttcaat cagctgctct ttttccatct
    46741 cagctaccca gcaaacgcag catttccgct tcgtcgatga cttcaatgcc cagttcctgc
    46801 gccttcgcca gtttagatcc tgcagcttca cccgctatca ccagatcggt tttcttcgac
    46861 acgctgcccg cgactttcgc gcccagttcg accagtcgag ctttagcgtc atcacgcgac
    46921 atctggctta agctgcccgt aagcaccacg gttttaccag caaacgggct gtcaatctct
    46981 tccgcgttga taacgatcgg cgcaggccag tgaacacctt ccgccaacag ctcgctgatg
    47041 acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca
    47101 acatcaggca ccttttgcag ctcttcaatc gaagcggctt ccagcacttc cagcgtgccg
    47161 aaatatgccg ccagacctgc tgcggtggcc tcgccgactt cacggatgcc aagtgcatag
    47221 aggaagcgag caaaggtggt ttctttcgct ttttccagcg cgttaaccac gttttgtgcc
    47281 gattttggcc ccatacgctc cagtccggtc agtttgcctg cggtgagttt gaacagatct
    47341 gccggagtgt ggacatattc tttttcaacc agctgatcga tgattttgtc gcccattccg
    47401 tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg actctttacg ctgcgcaccg
    47461 caaatcaggc caccggtaca gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa
    47521 ccacataccg gacaatgcgt cgggaataca acctcacggg tatcttccgg gcgttcagaa
    47581 agcacgacgt taaccacctg cgggatcacg tcgccagcgc ggcgaatcac cactttatcg
    47641 ccaatgcgta aaccaagacg ttcgatttca tccgcattgt gtaaggttgc gttactcacc
    47701 agcacgccag caacatggac aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc
    47761 ccaacctgaa actcgacgtc acgcacaaag gtcatctgct cctgcgccgg gaatttaaac
    47821 gctaccgccc agcgcggggc acgcgcgaca aagccaagct gctcctgctg ttccagtgag
    47881 ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg tcgggcggtc ttcttccact
    47941 ttgtgataga acgccagcac ttcttccgcc gattcacaaa gcgttacccg atcgctgacc
    48001 ggcaaccccc actttttaaa ttgcagtaaa cggccaagat gagtatccgg cagctcgcca
    48061 ccttccagaa caccaacgcc atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc
    48121 ggatcaagct gacgcagtga accagctgcc gcattacgtg ggttagcaaa aactttcccg
    48181 cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg cctgcggcag gaacacttca
    48241 ccacgcactt ccagacgcgc cgggatattc tctccgtgca gcttcagcgg aatggcgcga
    48301 atagtacgca cattagacgt gatatcttcc ccggtggtgc catcgccacg ggtcgcggca
    48361 ctgactaaaa cgccattttc atacagaata ctgacggcaa ggccatccag cttcagctca
    48421 cagcaccagg tgactttctc gttgtttttc agacggtcct gcacacgttt gttgaaagca
    48481 agaaagcttt cttcatcaaa aacgttatcc agtgacagca ttggtacttc atggcgtatc
    48541 tggctgaaag ccgccagcgg cgcagcacct acacgttggg taggcgaatc aggcgtaatc
    48601 agttctggat gtttggtttc cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg
    48661 tcgggaattt ccggcgcatc catcacatga taaagatatt catgatggcg aagcgtcgtt
    48721 cgcagttctg tcagttgttg ttcgattgat tccatatcgc accatcaatg ctaaaaaccc
    48781 ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc
    48841 tttgacttcg cggatgatgt cctggtactc gcgcaatttc tgcggagtca tcatacggcg
    48901 ctgatcgtca agcacgacac cgcccacttc atcggcaata tgctgcgcag attgcagcat
    48961 cagcttgaag ttctgcagct cgtcaccgta agacggtacc tgcataaaga tagtgacacc
    49021 cggagtagtg aaatccttca tttcaggatc aaaggttccc ggtttcacca tattcgccag
    49081 gctgaataac gccgggccgc tgccatccgg gctaagatga cgatggtaaa tattcatatc
    49141 gccaaaaatg aagcccgctt gttgaatgct gttaagaagc agttcaccgt ttagctcgct
    49201 accgtgatgc gccgcgacgt tcatgataat caccgcttct ttgcgcttcg gtttatccat
    49261 aactggagcc ggttccgcta caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg
    49321 gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc tgcggcgctg gcgcgacctg
    49381 tggcgaaact ggctgctgca acggctgttc aggctgcggc tgataggcag gctgctgcat
    49441 cggctgcgct ggacgcggag catgttgcgg cggtacctgc gcttcaggcg gctgctggat
    49501 cggttgacgc ggctgcgcag acgcataagg cggttggtac tggtgttgcg gcgacggacg
    49561 agcagcctca tgctcctgag cgttagccgg ggcatgattc acgcggtgaa cacgaacctc
    49621 accaacgccc tcatcatctt cgacatcctc gtcataagaa tcgtcgtcac gttttgactt
    49681 cattcgtttt aatggccgat cgcggaacat agaagatcgt tctttacggc tggtccagaa
    49741 accatgtacc agtaaagcga ttatggcgat cgcgccaaca atgattaata tcagacgcaa
    49801 atcctgcatc attatattct ctgttgttct aacaccttgc caccacggca aacatttact
    49861 cactaagagt atttgccgat tacctcaagt gcaagtgcac tattaacttt cacagcacaa
    49921 agatagatga aatcgtgctt tttgctgttt tttcgaacat atcctaactg tccattgcgc
    49981 aattacccgg taaaatacgc agaattttcc tgggattggt caaaaggagc tcatcctgat
    50041 tatggtttca tcattcacat ctgccccacg cagcggtttt tactattttg cgcaaggctg
    50101 gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt ttaccgctgc tggtcaatat
    50161 tttgttgatg gggggcgcat tctggtggct ctttacacag ctcgatgtct ggatcccgac
    50221 tctcatgagt tacgttccgg actggctaca atggctgagt tatctgttgt ggcctctggc
    50281 tgtcatctct gtgctgttag tgtttggcta tttcttctcc acgattgcta actggattgc
    50341 cgctccgttt aacggtttat tggctgaaca actggaagca cgattgactg gcgctacacc
    50401 gccagatacc gggattttcg gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg
    50461 gcaaaaattt gcctggtatc tgccgcgcgc aattgtatta ctaattcttt acttcatccc
    50521 tggtattggg caaaccgtcg cgccggtact gtggttcctg tttagcgcct ggatgttagc
    50581 catccagtat tgcgattacc ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac
    50641 cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt gctttaacca gcctgtttac
    50701 gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat
    50761 gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg taacaatcta ccggttattt
    50821 tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc ccttattcca tcttgcatgt
    50881 cattatttcc cttctgtata tagatatgct aaatccttac ttccgcatat tctctgagcg
    50941 ggtatgctac ctgttgtatc ccaatttcat acagttaagg acaggccatg agtaagattt
    51001 ttgaagataa ctcgctgact atcggtcaca cgccgctggt tcgcctgaat cgcatcggta
    51061 acggacgcat tctggcgaag gtggaatctc gtaaccccag cttcagcgtt aagtgccgta
    51121 tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac
    51181 tggttgaacc gaccagcggt aataccggga ttgcactggc ctatgtagct gccgctcgcg
    51241 gttacaaact caccctgacc atgccagaaa ccatgagtat tgaacgccgc aagctgctga
    51301 aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc
    51361 aaaaagcaga agaaattgtc gccagcaatc cagagaaata cctgctgctg caacaattca
    51421 gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg tccggagatc tgggaagata
    51481 ccgacggtca ggttgatgta tttattgctg gcgttgggac tggcggtacg ctgactggcg
    51541 tcagccgcta cattaaaggc accaaaggca agaccgatct tatctctgtc gccgttgagc
    51601 caaccgattc tccagttatc gcccaggcgc tggcaggtga agagattaaa cctggcccgc
    51661 ataaaattca gggtattggc gctggtttta tcccggctaa cctcgatctc aagctggtcg
    51721 ataaagtcat tggcatcacc aatgaagaag ccatttctac cgcccgtcgt ctaatggaag
    51781 aagaaggtat tcttgcaggt atctcttctg gagcagctgt tgccgcggcg ttgaaactac
    51841 aagaagatga aagctttacc aataagaata ttgtggttat tctaccatca tcgggtgagc
    51901 gttatttaag caccgcattg tttgccgatc tcttcactga gaaagaattg caacagtaat
    51961 gccagcttgt taaaaatgcg taaaaaagca cctttttagg tgcttttttg tggcctgctt
    52021 caaactttcg cccctcctgg cattgattca gcctgtcgga actggtattt aaccagacta
    52081 attattttga tgcgcgaaat taatcgttac aggaaaagcc aaagctgaat cgattttatg
    52141 atttggttca attcttcctt taacggcata atgtttaatg acgtacgaaa cgtcagcggt
    52201 caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg
    52261 gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga ctttagttcc acaacactaa
    52321 acctataagt tggggaaata caatgttcca gcaagaagtt accattaccg ctccgaacgg
    52381 tctgcacacc cgccctgctg cccagtttgt aaaagaagct aagggcttca cttctgaaat
    52441 tactgtgact tccaacggca aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct
    52501 gggcctgact caaggtaccg ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc
    52561 ggttgaacat ctggttaaac tgatggcgga actcgagtaa tttcccgggt tcttttaaaa
    52621 atcagtcaca agtaaggtag ggttatgatt tcaggcattt tagcatcccc gggtatcgct
    52681 ttcggtaaag ctctgcttct gaaagaagac gaaattgtca ttgaccggaa aaaaatttct
    52741 gccgaccagg ttgatcagga agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc
    52801 cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc
    52861 tttgaagggc atattatgct gctcgaagat gaggagctgg agcaggaaat catagccctg
    52921 attaaagata aacacatgac agctgacgca gctgctcatg aagttatcga aggtcaggct
    52981 tctgccctgg aagagctgga tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat
    53041 atcggtaagc gcctgctgcg caacatcctg ggcctgaaga ttatcgacct gagcgccatt
    53101 caggatgaag tcattctggt tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac
    53161 ctgaagaagg tgctgggttt catcaccgac gcgggtggcc gtacttccca cacctctatc
    53221 atggcgcgtt ctctggaact gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg
    53281 aaaaatgacg actatctgat tctggatgcc gtaaataatc aggtttacgt caatccaacc
    53341 aacgaagtta ttgataaaat gcgcgctgtt caggagcaag tggcttctga aaaagcagag
    53401 cttgctaaac tgaaagatct gccagctatt acgctggacg gtcaccaggt agaagtatgc
    53461 gctaacattg gtacggttcg tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt
    53521 ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa
    53581 cagtttgctg cttacaaagc agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt
    53641 accatggaca tcggcggcga caaagagctg ccatacatga acttcccgaa agaagagaac
    53701 ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc gtaaagagat cctgcgcgat
    53761 cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat tgcgcattat gttcccgatg
    53821 atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa
    53881 ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca
    53941 ccggctgccg caacaattgc acgtcattta gccaaagaag ttgatttctt tagtatcggc
    54001 accaatgatt taacgcagta cactctggca gttgaccgtg gtaatgatat gatttcacac
    54061 ctttaccagc caatgtcacc gtccgtgctg aacttgatca agcaagttat tgatgcttct
    54121 catgctgaag gcaaatggac tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca
    54181 cttctgttgc tggggatggg tctggacgaa ttctctatga gcgccatttc tatcccgcgc
    54241 attaagaaga ttatccgtaa cacgaacttc gaagatgcga aggtgttagc agagcaggct
    54301 cttgctcaac cgacaacgga cgagttaatg acgctggtta acaagttcat tgaagaaaaa
    54361 acaatctgct aatccacgag atgcggccca atttactgct taggagaaga tcatgggttt
    54421 gttcgataaa ctgaaatctc tggtttccga cgacaagaag gataccggaa ctattgagat
    54481 cattgctccg ctctctggcg agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc
    54541 ggaaaaaatc gttggtgatg gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc
    54601 tgtagacggt accattggta aaatctttga aaccaatcac gcgttctcta tcgaatctga
    54661 tagcggcgtt gaactgttcg tccacttcgg tatcgacacc gttgaactga aaggcgaagg
    54721 cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt
    54781 tgatctgccg ctgctggaag agaaagccaa gtctaccctg actccggttg ttatctccaa
    54841 catggacgaa atcaaagaac tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc
    54901 ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa atggcgccca
//
LOCUS       DAQSWQ010000017        53401 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.017, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000017 DAQSWQ010000000
VERSION     DAQSWQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.017"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="R8G18_001043"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="R8G18_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HEE9794813.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="R8G18_001044"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="R8G18_001044"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEE9794814.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="R8G18_001045"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="R8G18_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HEE9794815.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="R8G18_001046"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="R8G18_001046"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEE9794816.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="R8G18_001047"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="R8G18_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HEE9794817.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="R8G18_001048"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="R8G18_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HEE9794818.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="R8G18_001049"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="R8G18_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HEE9794819.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="R8G18_001050"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="R8G18_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HEE9794820.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="R8G18_001051"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="R8G18_001051"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HEE9794821.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="R8G18_001052"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="R8G18_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HEE9794822.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="R8G18_001053"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="R8G18_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HEE9794823.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="R8G18_001054"
     CDS             complement(13980..14846)
                     /locus_tag="R8G18_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HEE9794824.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="R8G18_001055"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="R8G18_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HEE9794825.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17473..17808
                     /locus_tag="R8G18_001056"
     CDS             17473..17808
                     /locus_tag="R8G18_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HEE9794826.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17966..18409
                     /gene="yjdN"
                     /locus_tag="R8G18_001057"
                     /pseudo
     CDS             17966..18409
                     /gene="yjdN"
                     /locus_tag="R8G18_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18542..19330
                     /gene="phnC"
                     /locus_tag="R8G18_001058"
     CDS             18542..19330
                     /gene="phnC"
                     /locus_tag="R8G18_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEE9794827.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19355..20371
                     /gene="phnD"
                     /locus_tag="R8G18_001059"
     CDS             19355..20371
                     /gene="phnD"
                     /locus_tag="R8G18_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEE9794828.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20497..21276
                     /gene="phnE"
                     /locus_tag="R8G18_001060"
     CDS             20497..21276
                     /gene="phnE"
                     /locus_tag="R8G18_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEE9794829.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21297..22022
                     /gene="phnF"
                     /locus_tag="R8G18_001061"
     CDS             21297..22022
                     /gene="phnF"
                     /locus_tag="R8G18_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HEE9794830.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22023..22475
                     /gene="phnG"
                     /locus_tag="R8G18_001062"
     CDS             22023..22475
                     /gene="phnG"
                     /locus_tag="R8G18_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEE9794831.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22472..23056
                     /gene="phnH"
                     /locus_tag="R8G18_001063"
     CDS             22472..23056
                     /gene="phnH"
                     /locus_tag="R8G18_001063"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEE9794832.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23056..24120
                     /gene="phnI"
                     /locus_tag="R8G18_001064"
     CDS             23056..24120
                     /gene="phnI"
                     /locus_tag="R8G18_001064"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HEE9794833.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24113..24958
                     /gene="phnJ"
                     /locus_tag="R8G18_001065"
     CDS             24113..24958
                     /gene="phnJ"
                     /locus_tag="R8G18_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HEE9794834.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPT
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24955..25713
                     /gene="phnK"
                     /locus_tag="R8G18_001066"
     CDS             24955..25713
                     /gene="phnK"
                     /locus_tag="R8G18_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HEE9794835.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25824..26504
                     /gene="phnL"
                     /locus_tag="R8G18_001067"
     CDS             25824..26504
                     /gene="phnL"
                     /locus_tag="R8G18_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HEE9794836.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26501..27637
                     /gene="phnM"
                     /locus_tag="R8G18_001068"
     CDS             26501..27637
                     /gene="phnM"
                     /locus_tag="R8G18_001068"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEE9794837.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27637..28194
                     /gene="phnN"
                     /locus_tag="R8G18_001069"
     CDS             27637..28194
                     /gene="phnN"
                     /locus_tag="R8G18_001069"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HEE9794838.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28181..28615
                     /gene="phnO"
                     /locus_tag="R8G18_001070"
     CDS             28181..28615
                     /gene="phnO"
                     /locus_tag="R8G18_001070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HEE9794839.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            28617..29375
                     /gene="phnP"
                     /locus_tag="R8G18_001071"
     CDS             28617..29375
                     /gene="phnP"
                     /locus_tag="R8G18_001071"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HEE9794840.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(29522..29851)
                     /gene="yjdP"
                     /locus_tag="R8G18_001072"
     CDS             complement(29522..29851)
                     /gene="yjdP"
                     /locus_tag="R8G18_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HEE9794841.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(29920..30237)
                     /locus_tag="R8G18_001073"
     CDS             complement(29920..30237)
                     /locus_tag="R8G18_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEE9794842.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            30490..32475
                     /gene="yjcS"
                     /locus_tag="R8G18_001074"
     CDS             30490..32475
                     /gene="yjcS"
                     /locus_tag="R8G18_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HEE9794843.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            32684..32959
                     /locus_tag="R8G18_001075"
     CDS             32684..32959
                     /locus_tag="R8G18_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794844.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            32978..34009
                     /gene="mdtN"
                     /locus_tag="R8G18_001076"
     CDS             32978..34009
                     /gene="mdtN"
                     /locus_tag="R8G18_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HEE9794845.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            34009..36060
                     /gene="mdtO"
                     /locus_tag="R8G18_001077"
     CDS             34009..36060
                     /gene="mdtO"
                     /locus_tag="R8G18_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HEE9794846.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            36057..37523
                     /gene="mdtP"
                     /locus_tag="R8G18_001078"
                     /pseudo
     CDS             36057..37523
                     /gene="mdtP"
                     /locus_tag="R8G18_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            37721..39868
                     /gene="fdhF"
                     /locus_tag="R8G18_001079"
     CDS             37721..39868
                     /gene="fdhF"
                     /locus_tag="R8G18_001079"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:38138..38140,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEE9794847.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            39962..40651
                     /locus_tag="R8G18_001080"
     CDS             39962..40651
                     /locus_tag="R8G18_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEE9794848.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(40730..42043)
                     /gene="gltP"
                     /locus_tag="R8G18_001081"
     CDS             complement(40730..42043)
                     /gene="gltP"
                     /locus_tag="R8G18_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HEE9794849.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(42385..42981)
                     /gene="nrfG"
                     /locus_tag="R8G18_001082"
     CDS             complement(42385..42981)
                     /gene="nrfG"
                     /locus_tag="R8G18_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HEE9794850.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(42978..43361)
                     /gene="nrfF"
                     /locus_tag="R8G18_001083"
     CDS             complement(42978..43361)
                     /gene="nrfF"
                     /locus_tag="R8G18_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HEE9794851.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(43354..45072)
                     /locus_tag="R8G18_001084"
     CDS             complement(43354..45072)
                     /locus_tag="R8G18_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEE9794852.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(45092..46048)
                     /gene="nrfD"
                     /locus_tag="R8G18_001085"
     CDS             complement(45092..46048)
                     /gene="nrfD"
                     /locus_tag="R8G18_001085"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HEE9794853.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(46045..46716)
                     /gene="nrfC"
                     /locus_tag="R8G18_001086"
     CDS             complement(46045..46716)
                     /gene="nrfC"
                     /locus_tag="R8G18_001086"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HEE9794854.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(46713..47279)
                     /gene="nrfB"
                     /locus_tag="R8G18_001087"
     CDS             complement(46713..47279)
                     /gene="nrfB"
                     /locus_tag="R8G18_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HEE9794855.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(47324..48760)
                     /gene="nrfA"
                     /locus_tag="R8G18_001088"
     CDS             complement(47324..48760)
                     /gene="nrfA"
                     /locus_tag="R8G18_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HEE9794856.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            49154..51112
                     /gene="acs"
                     /locus_tag="R8G18_001089"
     CDS             49154..51112
                     /gene="acs"
                     /locus_tag="R8G18_001089"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEE9794857.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            51312..51625
                     /locus_tag="R8G18_001090"
                     /pseudo
     CDS             51312..51625
                     /locus_tag="R8G18_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            51622..53271
                     /gene="actP"
                     /locus_tag="R8G18_001091"
     CDS             51622..53271
                     /gene="actP"
                     /locus_tag="R8G18_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HEE9794858.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT        12837 a        14704 c        13891 g        11969 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcggatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga aacccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttttg gcttttataa ttactgcaag
    17161 aaataatttt atatttagtg tgttgttttt tatcagaata aataacgtct tctgatgcgt
    17221 ttaaaacgtc agaaagataa aaatatcatg tgaataaaaa aaagaacaag tagagcatta
    17281 acattatctt aaataataaa tagaggcaaa aagattattt tctttttgcg tttcctttca
    17341 aatgaaaacg atcgtcgtct aaaatcagca gtacccccga caaactcagg gattttgtgt
    17401 ataattgcgg cctttttcgg caatctgccg ttttttggcg cttttgccct gctgactttt
    17461 gaggaaatcc acatgtcatt accacactgc ccaaaatgca actccgaata cacttacgaa
    17521 gataacggca tgtacatctg cccggaatgt gcctacgaat ggaacgacgc agaacctgca
    17581 caggaaagcg acgagctgat cgttaaagat gctaacggca atctgctggc tgacggcgac
    17641 agcgttacca tcattaaaga tctgaaggtg aaaggtagct cttcgatgct gaaaattggc
    17701 accaaagtga aaaacatccg cctggttgaa ggcgaccata acatcgattg caaaatcgac
    17761 ggttttggtc cgatgaaact gaaatctgag tttgtgaaaa agaactgatt gtattgtgat
    17821 cggtaagccg gataaggcgc tcgcgccgca tccggcaacg atgccgactg cctgatgcga
    17881 cgcttgcgcg tcttatcagg cagcctctca tctgtataaa tttcgaacta cacttaattg
    17941 gcttctctta actgaggtca ccatcatgcc gttaagtccc tacctctctt ttgccggtaa
    18001 ctgtgccgac gcaattgctt attatcaacg tacgttgggc gctgaactgc tctataaaat
    18061 cagcttcggc gaaatgccaa aatcagcgca ggacagcgcc gagaactgcc cttccggaat
    18121 gcaatttccc gataccgcca tcgcccacgc caatgtgcgc attgccggaa gcgacatcat
    18181 gatgagcgat gccatgccgt caggaaaagc cagctactcc ggctttacgc tggtgctcga
    18241 ttcgcaacag gtcgaagaag gaaaacgctg gtttgacaat cttgccgcta acggaaaaat
    18301 cgaaatggcc tggcaggaaa ctttctaggc gcatggcttt ggcaaagtca ccgataaatt
    18361 tggcgtaccg tggatgatta atgtcgtcaa acaacaacca acgcaataac ccgccgggag
    18421 gcccgccctc ccgcactgtc atcgaattcc cgttaactct tcatctgtta gtcactttta
    18481 attaaccaaa tcgtcacaat aatccgccac gatggagcca cttttttagg gaggctgcat
    18541 catgcaaacg attatccgtg tcgagaagct cgccaaaacc ttcaatcagc atcaggcgct
    18601 gcatgcggtt gatctgaaca ttcatcacgg tgaaatggtg gctctgcttg ggccgtcggg
    18661 ttccggcaaa tccacccttt tacgtcactt aagcggtttg attaccggcg ataaatccgc
    18721 cggcagccat atcgagctgc tgggccgcac agtccagcgc gaaggccgtc tggcgcgcga
    18781 tatccgcaaa agccgcgcca acaccggcta catcttccaa caattcaacc tggtgaaccg
    18841 cctgagcgta ctggagaacg tgctgattgg cgcactcggc agcacgccgt tctggcgcac
    18901 ctgttttagc tggtttaccc gcgagcagaa acaacgcgcg ttacaggcgc tgacccgcgt
    18961 tggcatggtg cattttgccc atcaacgcgt ttccaccctc tccggcggac agcagcagcg
    19021 tgtggcgatt gcccgcgcgc tgatgcagca ggcgaaggtg attctggccg atgaacccat
    19081 cgcctcgctg gacccggaat ccgccggcat cgtgatggac accctgcgcg acatcaatca
    19141 gaacgacggc atcaccgtgg tcgtcacgct gcatcaggtg gattacgccc tgcgctactg
    19201 cgaacgcatc gtcgccctgc gccaggggca cgttttctac gacggcagca gccaacagtt
    19261 tgataacgaa cgttttgacc atctctaccg cagcattaat cgcatcgaag agaacgcgaa
    19321 agctgcctga catccccatc attgaggaaa acgaatgaac gctaagataa ttgcctcgct
    19381 ggccttcacc agcatgttca gcctcagcac cctgttaagc ccggcacacg ccgaagagca
    19441 ggaaaaggcg ctgaatttcg gcattatttc aacggaatca cagcaaaacc tgaaaccgca
    19501 atggacgcca ttcttacagg atatggagaa gaagctgggc gtgaaggtga acgccttctt
    19561 tgccccagac tacgcaggca ttatccaggg aatgcgcttc aataaagtgg atatcgcctg
    19621 gtacggcaac ctgtcggcaa tggaagcggt ggatcgcgcc aacggccagg tcttcgccca
    19681 gacggtcgcg gcggatggat cgccaggtta ctggagcgtg ttgatcgtca acaaagatag
    19741 tccgatcaac aacctgaacg atctgctggc gaagcggaaa gatctcacct tcggcaatgg
    19801 cgatcctaac tccacctctg gcttcctcgt ccccggttac tacgtctttg ccaaaaacaa
    19861 tatctccgcc agcgacttta agcgcaccgt caacgccggg catgaaacca acgcgctggc
    19921 cgtcgccaac aagcaggtgg atgttgccac caacaacacc gaaaacctcg acaagctgaa
    19981 aacctccgcg ccagagaagc tgaaagaact gaaggtgatc tggaagtcgc cgctgatccc
    20041 aggcgatccg atcgtctggc gcaagaatct ctccgaaacc accaaagaca agatctacga
    20101 cttctttatg aactacggaa aaacgccgga agagaaagcg gtgctggaac gcctgggctg
    20161 ggcaccgttt cgcgcctcca gcgacctgca actggtgccg attcgccagc tcgcactgtt
    20221 taaagagatt cagggcgtaa aaagcaataa cggactgaat gagcaggaca agctggcgaa
    20281 aaccaccgag attcaggcgc agctggatga cctggaccgc ctgaacaacg cgttaagcgc
    20341 gatgagttcg gtgagtaaag cggtgcagta atgtgctcga tcggtcccct ctcccttgag
    20401 ggagagggtt agggtgaggg ggaaatgcgc acgctgatgc cctcaccccg gccctctccc
    20461 acagggagag ggagaaaaca ttaaacggag ccaaacatgc aaaccatcac catcgcccca
    20521 cccaagcgca gctggttctc gcttctgagc tgggccattg ttctcgccgt gttggtcgtc
    20581 tcgtggcagg gcgcggaaat ggccccgctt acgctgatca aagacggcgg caacatggcg
    20641 accttcgccg ccgacttctt cccgcccgat ttcagccagt ggcaggatta cctcaccgaa
    20701 atggccgtca cgctgcaaat cgccgtctgg ggcaccgcgc tggcagtggt tctctccatc
    20761 cccttcggcc tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca gcccgttcgc
    20821 cgcctgatgg acgcctgccg cgccattaac gaaatggtct tcgccatgct gttcgtggtc
    20881 gccgtcggtc tcggaccgtt cgctggcgtg ctggcgctat ttattcacac caccggcgtg
    20941 ctctcgaaac tgctttccga agcggtggaa gcgattgaac ccggcccggt ggaaggcatt
    21001 cgcgccaccg gcgccaacaa gctggaagag atcctttacg gcgtgctgcc acaggtaatg
    21061 ccgctgctga tctcctactc cctctatcgc ttcgaatcca acgtccgctc ggcgaccgtc
    21121 gtcggcatgg tcggtgcagg cgggatcggc gtcaccctgt gggaagcgat tcgcggtttc
    21181 cagttccaac aaacctgcgc cctgatggtg cttatcatcg tcacggtcag cctgctggat
    21241 ttcctctctc aacggttgcg taagcacttt atctgataag tgaggcattg atatctatgc
    21301 acttgtctac acatccgacc agctacccaa cacgctatca ggagatagcc gcaaaacttg
    21361 agcaggagct tcgtcaacac tatcgctgcg gcgactatct tcccgccgag cagcaactgg
    21421 cggcgcgctt tgaggtaaac cgccacaccc tgcgccgcgc catcgaccaa ctggtggaaa
    21481 aaggctgggt acagcgccgt cagggcatcg gcgtgctggt gctgatgcgc ccgttcgatt
    21541 acccgctcaa cgcccaggcg cgttttagcc agaacctgct ggaccagggc agccatccca
    21601 ccagcgaaaa actgctgtcg gtattgcgcc ccgcgtccgg tcacgttgct gacgcgctgg
    21661 ggattaccga gggtgagaac gttatccatt tgcgcaccct gcgtcgcgtc aacggcgtcg
    21721 cgctctgttt aatcgaccac tactttgcgg acctcaccct ctggccgaca ctgcaacgtt
    21781 tcgacagcgg ttcgctgcac gattttctgc gcgagcaaac cggaattgcg ctgcgccgca
    21841 gccagacgcg gatcagcgcc cgccgcgccc aggccaaaga gtgccagcgc cttgaaatcc
    21901 cgaatatgtc gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt gaaagcagcc
    21961 cggcggagta ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact atggagcact
    22021 gaatgcacgc agataccgcg acccgccagc actggatgtc cgtactggcg cacagccaac
    22081 cggctgaact ggcagcccgc ctgaacgcgc tgaacatcac cgccgactat gaagtgatcc
    22141 gcactgccga aactggcctg gtgcagattc aggcgcgaat gggcggtacc ggcgaacgct
    22201 tttttgccgg cgacgccacg ctgacccgtg ccgccgtacg cctgactgac ggcaccctcg
    22261 gctacagctg ggtgctgggg cgtgataaac agcacgccga acgctgcgcg ctgattgatg
    22321 cgctgatgca gcaatctcgc cactttcaaa acttatcaga aacccttatt gccccgctgg
    22381 acgctgaccg tatggcacgc attgccgcac gccaggccga agtgaacgcc agccgggtcg
    22441 acttctttac gatggttcgc ggagacaacg catgaccctg gaaaccgctt ttatgcttcc
    22501 cgtgcaggat gcccagcaca gttttcgtcg cctgttaaag gccatgagcg agccgggcgt
    22561 gattgtcgcc ctgtatcagc tcaaacgcgg ctggcagccg ctgaatatcg ccaccaccag
    22621 cgtgctgctg acgctggccg ataacgacac gccggtgtgg cttgccacgc cattaagtaa
    22681 cgatatcgtc aatcagagcc tgcgttttca taccaacgcg ccgctggtca gccagccgga
    22741 acaggcgacc ttcgcggtga cggatgaggc gatttccagc gaacagctca acgccctttc
    22801 caccgacacc gccgttgcgc cggaagcggg cgcgacgctg attttacagg tcgccagcct
    22861 gagcggcgga cgcatgttgc gtctcaccgg cgcgggcatt gccgaagaac gaatgatcgc
    22921 tccgcagctg ccagagtgca ttctgcacga actcaccgag cgcccgcacc cgttcccgct
    22981 cggcatcgac ctaatcctga cctgcggcga gcgcctgctg gctatcccgc gaaccactca
    23041 tgtggaggtg tgctgatgta cgttgccgtg aaagggggcg aaaaggcgat cgacgccgcc
    23101 cacgccctgc aagagagccg acgccggggc gataccgatt tgcccgaact gagcgtcgcc
    23161 cagattgaac agcagcttaa cctcgcggtg gatcgcgtga tgacagaagg cggcattgcc
    23221 gaccgcgaac tggcggcgct ggcgctgaaa caggccagcg gcgataacgt cgaagcgatt
    23281 ttcctgctac gcgcctaccg caccacgctg acgaaactgg cggtaagcga gccgctcgac
    23341 accacggaga tgcgtctcga acggcgtatt tcagcggttt ataaagatat tcccggcggc
    23401 cagctgcttg gcccaaccta cgactacacc catcgcctgc tcgatttcac tctgctggca
    23461 aacggcgaag cgccgacgct gaccaccgcc gacagcgaac aacagccgtc gccgcacgtt
    23521 ttcagcctgc tggcgcgtca ggggctggcg aagtttgaag aggatagcgg agcacagccg
    23581 gatgacatca cccgcacgcc gccggtttac ccctgttcac gttcttcccg tttgcagcag
    23641 ttaatgcgcg gcgacgaagg ctatttgctg gcgctggctt actccaccca gcgcggttac
    23701 gggcgcaacc acccgttcgc gggcgagatc cgcagtggtt acatcgacgt gtcgattgtg
    23761 ccggaagagc tgggatttgc ggtaaacgtc ggcgaactgc tgatgaccga gtgtgaaatg
    23821 gtcaacggtt ttatcgaccc gccggatgag ccgccgcact tcacgcgcgg ctacgggctg
    23881 gtattcggca tgagcgagcg caaagcgatg gcaatggcgc tggttgaccg tgcgttgcaa
    23941 gccccggaat acggcgagca cgcgacaggc ccggcgcagg atgaagagtt cgtgctggcg
    24001 catgccgaca acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc gcactacgtc
    24061 gatttccagg ccgaactgga gctactcaaa cgtctgcaac aggagcagaa ccatggctaa
    24121 tctgagcggc tacaactttg cctacctcga cgagcagacc aaacgcatga tccgccgcgc
    24181 catcttaaaa gcggtggcga tccccggtta tcaggtaccg tttggcgggc gcgagatgcc
    24241 gacgccgtac ggctggggaa ctggtggtat ccagctcacc gccagcgtga ttggcgaaag
    24301 cgacgtgttg aaggtgattg accagggtgc ggatgacacc accaacgccg tgtcgattcg
    24361 caacttcttt aagcgcgtga ccggggtaaa caccactgaa tgtacggacg atgcgacgct
    24421 tatccagacg cgtcaccgca tccccgaaac gccgctgacc gaagatcaga tcattatctt
    24481 ccaggtgcca atcccggaac cgctgcgctt tatcgagccg cgcgaaacgg agacccgcac
    24541 tatgcacgcg ctggaggagt acggcgtgat gcaggtgaaa ctgtatgaag atatcgcccg
    24601 cttcggtcat atcgccacca cctatgctta tccggtgaag gtgaacgggc gctacgtgat
    24661 ggacccgtcg ccgatcccga aattcgataa cccgaaaatg gacatgatgc ccgccctgca
    24721 gctgttcggc gcagggcgcg agaagcgcat ctatgcggtg ccgccgttta cccgcgtgga
    24781 aagtctcgat ttcgacgatc acccgttcac cgttcagcag tgggatgagc catgcgccat
    24841 ctgcggatcg acccacagct atcttgatga agtggtgctg gatgacgccg gaaaccgcat
    24901 gtttgtctgc tccgataccg attattgccg ccaacagagc gaggcgaaaa gccaatgaat
    24961 caaccgttac tttcggtcaa taacctgacc cacctttacg cgccgggcaa aggctttagc
    25021 gatgtctctt ttgatttatg gccgggggaa gtgctgggca ttgtcgggga atccggctcc
    25081 gggaagacca cgctgctgaa gtcgatctcc gcgcgtctga cgccgcagca gggggagatt
    25141 cactatgaaa accgttccct ctatgcaatg agcgaggccg accgccgtcg cctgctgcgc
    25201 accgaatggg gcgtggtgca tcagcatccg ctcgacggcc tgcgccgcca ggtgtcggca
    25261 ggcggcaata tcggcgagcg gctgatggcg accggggcgc gacattacgg cgatattcgc
    25321 gctaccgcgc agaagtggct ggaagaggtg gagattcccg ccaaccgtat cgacgacctg
    25381 ccgaccacct tttccggcgg tatgcagcag cgtttgcaga ttgcccgcaa cctggtgacg
    25441 catccgaagc tggtgtttat ggatgaaccg accggcgggc tggatgtgtc ggtgcaggcc
    25501 cgcctgctcg acctgctgcg cggactggtg gtggagctga acctcgcggt agtgattgtc
    25561 acccacgatt taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat gaagcagggg
    25621 caagtggtgg agagtgggtt aaccgaccgc gtgctcgacg acccgcatca tccgtataca
    25681 cagctgctgg tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg cgtaaacgcc
    25741 ttatccggcc tacaaatgcg ctccccgtag gtcggataag acgcgtcagc gtcgcatccg
    25801 acacccgaac cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa accttcatcc
    25861 tgcaccagca aaacggcgtg cgcctgcccg tcctcaaccg cgcctcgctc atcgtcaacg
    25921 cgggcgaatg cgtggtgctc cacggccatt ccggcagcgg aaaatcaacc ctgctacgct
    25981 cgctgtacgc caactatctg cccgacgaag ggcaaatcca gatcaaacac ggtgacgaat
    26041 gggtcgatct ggtcaccgcg ccagcgcgca aagtggtgga gatccgcaaa accaccgttg
    26101 gctgggtgag ccagtttctg cgcgtcatcc cgcgtatttc ggcgctggaa gtggtgatgc
    26161 agccgctact cgataccggc gtaccgcgtg aagcctgcgc cgctaaagcc gcgcgtctgc
    26221 tcacccgcct gaacgtgccg gaacgcctgt ggcatctggc gccatcgacc ttctccggcg
    26281 gcgaacagca gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc attctgctgc
    26341 tcgacgaacc taccgcctcg ctggacgcca aaaacagcgc cgccgtagtc gagctgattc
    26401 acgaagccaa agcccgtggc gcggccatcg tcggcatctt ccacgacgaa gccgtacgta
    26461 atgacgttgc cgaccgcctg cacccaatgg aagcctcttc atgattatca ataacgtcaa
    26521 attggtgctg gaaaacgaag tggtacacgg ttcactggag gtgcaggacg gcgaaatccg
    26581 cgcctttgcc gaaagccaga gccgcctgcc ggaggcgatg gacggcgaag gcggctggct
    26641 gctgccgggg ctgattgagc tgcataccga taatctggat aaattcttca ccccgcgccc
    26701 gaaagttgac tggcctgccc actcggcgat gagcagccac gacgcgctga tggtggcgag
    26761 cggcatcacc accgtactgg atgccgtggc aattggcgac gtgcgcgacg gcggcgatcg
    26821 gctggtgaat ctggagaaga tgatcaacgc catcgaagag acgcagaaac gcggcgtcaa
    26881 ccgcgccgag caccgtctgc atctgcgctg cgaactgccg catcacacca cgctgccgct
    26941 gtttgaaaaa ctggtgcagc gcgagccggt gacgctggtg tcgctgatgg accactcgcc
    27001 gggccagcgc cagttcgcca atcgcgagaa gtatcgcgaa tattatcagg gcaaatactc
    27061 cctcactgat gcgcagatgc agcagtacga agaagagcaa ctggcgctcg ccgcacgctg
    27121 gtcgcagccg aatcgcgaat ccatcgccgc cctgtgccgc gcgcgaaaaa ttgcgcttgc
    27181 cagccacgat gacgccaccc acgcccacgt tgctgaatct caccagcttg gcagcgtgat
    27241 cgccgaattt cccaccacgt tcgaagcggc ggaagcctcg cgcaagcatg gcatgaacgt
    27301 gctgatgggc gcgccgaata ttgtgcgcgg cggctcgcac tccggcaacg tggcagccag
    27361 tgaactggcg cagcttggcc tgctggatat cctctcttcc gactactacc ccgccagcct
    27421 gctcgatgcg gcatttcgcg tcgccgatga cgagagcaac cgctttacgc tgccgcaggc
    27481 ggtgaagctg gtgactaaaa atccagcgca ggcgcttaat ctccaggatc gcggggtgat
    27541 tggcgagggc aaacgcgcgg acttggtgct ggcgcatcgc aagggcaatc acattcatat
    27601 cgaccacgtc tggcgtcagg gtaaaagggt gttctgatga tgggaaaact gatttggtta
    27661 atggggccgt ccggctccgg gaaagatagc ctgctggcgg aactccgcct gcgggaacaa
    27721 actcagctac tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg aagcgaaaac
    27781 catatcgctc tgagcgagca ggagtttttt acccgcgcgg ggcagaacct actggcctta
    27841 agctggcacg ctaacggtct gtattatggc gtcggcgtcg agatcgacct ctggctgcac
    27901 gccggattcg acgtgctggt caacggctca cgcgcccatc tgccgcaggc gcgggcgcgc
    27961 tatcaatcgg cgctgctgcc cgtctgttta caggtttcgc cggagatcct ccgccagcgc
    28021 ctggaaaacc gtggccgtga aaacgccagt gaaattaacg cccgcctggc gcgcgccgcc
    28081 cgctatactc cacaggattg ccatacgctc aacaatgacg gcagcctgcg ccagtcggtc
    28141 gacacgctgc tgacgctgat tcatcagaag gagaaacacc atgcctgctt gtgagcttcg
    28201 cccggccacg cagtatgata ccgacgcggt ttacgcgctg atttgtgagc taaaacaggc
    28261 ggagtttgac caccacgcat ttcgcgtggg ctttagcgcc aatctgcgcg acccgaacat
    28321 gcgctatcat ctggcgctgc tggatggcga ggttgtcggc atgatcggcc tgcatttgca
    28381 gtttcatctg catcatgtca actggatcgg cgaaattcag gaactggtag tgatgccgca
    28441 ggcgcgcggc ctgaacgtcg gtagcaagtt gctggcgtgg gcagaagaag aagcccgcca
    28501 ggccggggcc gaaatgaccg agctttcgac caacgtgaag cgccacgacg cgcaccgttt
    28561 ctatctgcga gaaggctacg agcagagtca cttccgcttc accaaggcgc tgtaacatga
    28621 gtctgaccct cacgctcacc ggcaccggcg gcgcacaggg cgttccggca tggggctgcg
    28681 agtgcgcggc ctgcgaccga gcgcggcgct cgccgcagta tcgccgccaa ccgtgcagcg
    28741 gcgtagtgaa gtttaacgag gcgatcaccc tgatcgacgc cgggctgcac gatctcaccg
    28801 atcgctggtc tcccggatcg ttccagcagt ttttgctgac gcattatcat atggatcacg
    28861 tccaggggct gtttccgctg cgctggggcg tgggcgatcc gatcccgatc tacggcccgc
    28921 cggatgaaca gggctgcgac gatctgttta aacatcccgg gctgcttgat ttcagccaca
    28981 cggtggaacc gtttgtggtg tttgatttgc aggggttgca ggtcacgccg ctgccgctta
    29041 accactcaaa actcactttc ggctatttac tggaaacggc gcacagccgg gtggcgtggc
    29101 tgtctgacac cgctggcttg ccggaaaaaa cgctgaaatt tttacgcaat aatcagccgc
    29161 aggtaatggt gatggattgc agtcacccgc cgcgcgcgga tgcaccgcgt aatcactgtg
    29221 atttaaatac cgtgcttgcg ctgaatcagg ttatccgctc gccacgggtg attctgaccc
    29281 atatcagcca ccagtttgat gcgtggctga tggaaaacgc actaccgtca gggtttgagg
    29341 tggggtttga tgggatggag attggggtgg cgtgatgaga gggaatgtgc gcgctggccc
    29401 cctcacccta accctctccc cagaggggcg aggggaccga ttgtgctcga tattgaatat
    29461 tgcgctcgtt ttctccctct ccccattggg gtgaggggcg atgcctgctc catacccaac
    29521 ctcatcgccc atactcatct tccattctcc gctcttcatc ctccagttgc cgacgctcct
    29581 gatcaagctg gcgctggcga tcgtccagct gcctgcggcg atcttcaaac tggcggcggc
    29641 ggtcgtcata ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg tcgtcgcgcg
    29701 aatcttcata gtctcgccca cggtcagggt tataagcgtc attaatcgcc tgctgaatat
    29761 taccaatagt atcgtcgata atatcagcct gggccggaac ggtggacagc gtgagcaggg
    29821 tgaataaaag aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa actctatcca
    29881 cattagtcat tactcatcca tgcaagtagt ggatgaatct caatttctcc gctgctctat
    29941 tgccgtaatc gcctccacgc gttgttgatg acgaccgcct tcgtactgtg cgcccagcca
    30001 cgcatccaca atcatttttg ccagttcgag gccaaccact cgtgaaccaa aagccagtgc
    30061 aacttcactg gcataatgcg gataatcagt gcgctctgac gacctggtgt ctttatcaat
    30121 cacctcactc attgaccatg aataaaaggc tggcacagca acgcagaatc ctgcggtgcc
    30181 agcgacatga ccgcccattg ctccggtacg ttttttaaaa agaaacaccg gttgcattga
    30241 taaattgtgc tcagtatttg ccgaagtaca atatatataa taaatatata aaacacctca
    30301 tattatttac agagataata aattcgcgta attttccctc ttcagcacaa atgcctaata
    30361 aaatggatcg ttcagctcac acttccactt attaaagtcg caaattccca attatgataa
    30421 ccccagcttt aaatgacgta ttttgcatgg attacatatt tacgacataa atgataaatg
    30481 gagatttaca tgaataactc tcggttattc cgtttgagca gaattgttat tgcgttaact
    30541 gccgccagcg gcatgatggt aaataccgct aacgcgaaag aggaagcgaa agccgccact
    30601 caatataccc aacaggttaa tcagaattac gccaaatcat taccgtttag cgatcgtcag
    30661 gattttgacg acgctcagcg tggatttatc gctccgctgc tggatgaagg tattctgcgc
    30721 gatgctaatg gcaaaccata ttatcgcggg gaagattata aatttgatat caatgcgccc
    30781 gcgcccgaaa ccgttaaccc tagcctgtgg cgtcagtcgc agttaaacgg tatttccggc
    30841 ctgtttaaag tcaccgacag aatgtaccag gttcgcggtc aggatatctc caacatcacc
    30901 tttatcgaag gtgataccgg gattattgtc atcgacccgc tggtgacgcc accgagcgca
    30961 aaagccgccc ttgaccttta cttccaaaat cgcccgcaaa aaccgattgt tgcggttatt
    31021 tatacccaca gccataccga ccactatggc ggcgtgaaag gcattatctc tgaagctgat
    31081 gtgaaatccg gtaaagtgca ggttatcgcc cctgctggct ttatggacga agccatcagc
    31141 gaaaacgtac tggcgggtaa tattatgagc cgccgtgcac tttactccta cggcctgctg
    31201 ctggcgcata accctcaggg taacatcggc aatggtcttg gcgtaacgct ggcatcgggc
    31261 tacccgagca tcatcgcacc gaacaaaacc atcaccaaaa ccggtgagaa gatgattatc
    31321 gacggcctgg agtttgactt cctgatgacc ccaggtagcg aagcaccagc cgaaatgcac
    31381 ttctatattc cggccctgaa agcgctgtgt accgccgaga acgccacgca taccctgcac
    31441 aacttctaca ctctgcgcgg cgcgaaaacc cgcgacacca gcaagtggac cgagtatctg
    31501 aacgaaacgc tggatatgtg gggtaacgac gcggaagtcc tgtttatgcc gcacacctgg
    31561 ccggtctggg gcaataagca tatcaatgat tatattggta aatatcgcga tactatcaag
    31621 tacattcacg accagaccct gcacctggcg aaccagggct acaccatgaa tgaaatcggc
    31681 gacatgatta aactgccgcc tgcacttgcc aataactggg ccagccgtgg ctattacggt
    31741 tctgtcagcc acaacgcccg cgcggtgtat aacttctatc ttggctatta cgacggtaac
    31801 ccggctaacc tgcatccgta tggtcaggtg gagatgggta aacgttacgt gcaggcgctg
    31861 ggcggttctg cccgtgtcat caacctggcg caagaagcga acaagcaagg tgattaccgc
    31921 tggtcggcag aactgctgaa acaggtgatt gccgccaacc cgggtgacca ggtcgcgaag
    31981 aatctgcaag cgaataactt tgaacagctg ggctatcagg ccgagtccgc cacctggcgc
    32041 ggtttctacc tgaccggcgc gaaagagctg cgcgaagggg tgcataagtt cagccacggc
    32101 accatcggtt ccccggacac cattcgcggg atgtcggtcg aaatgctgtt cgactttatg
    32161 tccgttcgcc tcgatagcgc gaaagccgcg ggtaaaaata tcagcctgaa cttcaatatg
    32221 agcaatggcg ataacctcaa cctgacgctg aacgatagcg tgcttaacta ccgtaaaaca
    32281 ctgcaatccc aagctgacgc ctctttctac atcagccgtg aagatctgca cgccgtgctg
    32341 accgggcaag ccaaaatggc ggatctggta aaagcgaaga aagccaaaat tattggcaat
    32401 ggcgcgaaac tggaagaaat tatcgcctgt ctggataatt tcgatttgtg ggtgaatatc
    32461 gtaaccccaa attaatgtca ctcgcttttg ggggcgctga agcccccaaa agcatttatt
    32521 ttaaaaatat atacagccaa ttaataaata aaatatataa gttatgaatg cggtgactga
    32581 ataaagctat ttttattcat cgagaaaata gccatactga cgccttcaat ggatattgat
    32641 attcactaca gatattgttc gcagcagaca ggatgtattt aatatgccaa cagttctctc
    32701 tcgcatggcg atgcaactca aaaaaacagc ctggataatt cccgtcttca tggtttcggg
    32761 atgctcatta tctccggcaa tcccggtgat cggtgcttat tatcccagct ggtttttctg
    32821 cgccattgcc agccttattt tgatgctcat cacgaggcga gttattcagc gggcaaatat
    32881 caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg ccatgctgtt
    32941 ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc cgaaaaaagc
    33001 tcctcgcagt aaattccctg ctctgttagt ggttgcattg gcgctggttg cccttgtttt
    33061 cgttatctgg cgcgtagaca gtgcgccatc aactaatgac gcttacgcgt cagcagatac
    33121 cattgatgtg gtgccggaag tcagcggccg cattgtagaa ctggcggtca ccgacaacca
    33181 ggcagtcaaa cagggcgatt tgctgttccg cattgacccg cgcccgtacg aagccaatct
    33241 ggcgaaagct gaagcctccc tcgcggcgct ggataagcaa attatgctca cccagcgtag
    33301 cgttgatgcg caacagtttg gtgccgactc gattaatgcc acggtagaaa aagcccgtgc
    33361 cgccgcgaaa caggccacag atacattacg ccgtaccgag ccattactga aagagggttt
    33421 tgtctcagcg gaagacgttg accgtgcaag aactgcgcag cgcgccgcag aagccgatct
    33481 taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg tcgatgcgct
    33541 ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactgc atctggaaat
    33601 ggcgaccgtt cgcgcgccgt ttgatggccg ggtcatttcc ctcaaaacct ccgtcgggca
    33661 atttgcttct gccatgcgcc ctatttttac cctaatcgac actcgtcact ggtacgtgat
    33721 cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg caacgattcg
    33781 cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg gctacggcgt
    33841 gctaccggat gacggcggcc tggtgctggg cggcctgccg aaagtttctc gctctattaa
    33901 ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac ctgacccgga
    33961 aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat gagcgcgctc
    34021 aactccctgc cattaccgat ggtcaggctg ctggcgttct ttcatgaaga gttaagcgag
    34081 cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct gctggtgatt
    34141 ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt gctgttttac
    34201 ggtattcagt cgaacgcgtt ttacaccaaa tttgtggcga tcttgtttgt ggttgccacg
    34261 gtgctggaga tcggcagcct gtttttgatc tacaaatggt catacggcga accgttgatc
    34321 cgattgatca tcgccgggcc gatcctgatg ggccgcatgt ttttgatgcg cacccatcgg
    34381 ctgggactgg tctttttcgc tgtcgccatt gtcgccattt acgggcaaac cttccccgcc
    34441 atgctcgact atccggaagt ggtcgtgcgc ttaacgctgt ggtgtatcgt tgttggcctc
    34501 tatccaacct tgctgatgac gttaatcggc gtgctgtggt ttcccagtcg tgccatttcg
    34561 caaatgcatc aggcgcttaa tgatcggctt gatgatgcca ttagtcacct gacagacagc
    34621 ctcgcaccgc tacccgaaac gcggattgaa agagaggcgc tggcgctgca aaaactgaat
    34681 gtcttttgtc tcgcggacga tgccaactgg cgaactcaaa gcgcatggtg gcaaagctgc
    34741 gtggcaacgg taacctacat ttactcgacg ctgaatcgct acgatcccac ctcttttgct
    34801 gattctcagg caattattga atttcggcaa aaattagcct cagaaatcaa caagctgcag
    34861 catgccgttg ctgaaggtca gtgctggcaa agcgactggc ggatcagtga aagtgaagcg
    34921 gtggcggcac gggaatgtaa cctggagaat atctgccaga cgttgttaca actgggccag
    34981 atgaacccga atacgccgcc aacgcccgcc gccaaaccgc catcaatggt cgccgatgct
    35041 tttaccaatc cagactatat acgctacgcg gtaaaaacat tgctcgcctg tttgatctgt
    35101 tacacctttt acagcggcgt ggactgggaa ggcattcaca cctgtatgct gacctgcgtg
    35161 atcgtctcta atccaaatgt cggttcgtcg taccagaaga tggtgctgcg ttttggcggg
    35221 gccttttgcg gcgcgattct ggcgctgtta ttcacgctac tggtcatgcc ctggctggac
    35281 aatattgtcg aattgctgtt tgtgctggca ccgattttcc tgttgggcgc atggattgcc
    35341 accagctctg aacgctcttc ttatatcggc acacagatgg tggtcacctt cgcgctcgcc
    35401 acgctcgaaa acgtttttgg cccagtgtac gacctggtgg aaattcgcga tcgcgccctg
    35461 ggtatcatca ttggtaccgt ggtgtccgcg gtgatttaca cctttgtctg gcctgaaagt
    35521 gaagcgcgca cgctgccgca aaaactggct ggcgcgctgg gtatgttaag taaagtaatg
    35581 cggatcccac gccagcagga agtcacggct ctgcgcactt atctgcaaat tcgtatcggt
    35641 ctgcatgcgg cgtttaatgc ctgtgaagag atgtgccaac gcgtggcgct ggagcgtcaa
    35701 ctggacagcg aagaacgcgc cttactgatt gaacgttcgc aaacggttat tcgtcagggc
    35761 cgcgatatcc ttcacgcctg ggatgccacc tggaattcgg cgcaggcgct ggataacgca
    35821 ctacagccgg acagagcagg tcagtttgcc gacgccctgg aaaaatacgc tgccggtctg
    35881 gcaaccgcac tcagccgttc tcctcaaata acgcttgaag agacacccgc ctcgcaggcc
    35941 atcctgccca ccttattaaa acaggagcaa cacgtctgcc agcttttcgc ccgcttgcca
    36001 gactggacag ccccggcatt aacgcccgcc acggaacagg cacaaggagc cacgcaatga
    36061 tcaatcgtca actttcacgt ctgctgttgt gcagcattct cggcagcacg acgctgattt
    36121 ccggctgtgc cttggtacgc aaggattctg cgcctcatca acagctcaaa ccggaacaaa
    36181 tcaaactggc cgatgatatt catcttgcca gctccggctg gccgcaggcg cagtggtgga
    36241 aacaactcaa tgacccgtag ctggatgcgc tgatccaacg gacgctaagt ggttcacaca
    36301 ccctcgccga agcgaaactg cgggaagaaa aagcgcagtc ccaggccgat ttgttagatg
    36361 ccggttcaca attacaggtc gcagcgttag ggatgctcaa ccgccaacgc gtctcggcga
    36421 acggcttttt aagcccttat gcgatggatg cgcccgcact gggtatggac gggccgtact
    36481 atacggaagc cacagtaggt ttgtttgccg gactggatct tgatttgtgg ggtgtgcatc
    36541 gctcagcggt tgccgccgcc attggcgcgc ataatgccgc gctggcagaa accgcagcag
    36601 tagagctatc gctgaccacg ggcgtagcgc agctttatta cagtatgcag gccagctatc
    36661 agatgctcga tctgttagaa caaactcgcg atgtgattga ttacgcggtg aaagcgcacc
    36721 agagtaaagt ggcgcacggt ctggaagcac aagtgccttt ccacggcgcg cgggcacaga
    36781 ttctggcggt cgataaacaa attgctgccg tcaaagggca aatcaccgaa acgcgagaat
    36841 ctctgcgtgc attgattggc gcgggggcca gcgatatgcc ggagatcaaa ccggtggcat
    36901 taccacgagt ccagaccggc attccggcga cactctctta tgagttgctc gccagacgcc
    36961 cggatctgca agccatgcgc tggtatgttc aggcgtcatt agatcaggtg gattccgcgc
    37021 gggcgctgtt ctacccgagc tttgatatca aagcgttttt cggccttgat gccatccacc
    37081 tggatacctt attcaaaaaa accagtcgcc agttcaactt catcccgggt ctgaaattgc
    37141 cgctgtttga cggtggacgg ttgaatgcca atctcgaagg cacgcgcgcc gccagcaaca
    37201 tgatgattga acgttacaac cagtcagtac tgaacgcggt gcgtgacgtt gccgtcaacg
    37261 gcacgcgtct gcaaacgctc aacgacgagc gagaaatgca ggctgaacgc gtggaagcaa
    37321 cgcgcttcac ccagcgcgct gccgaggccg cctatcagcg cggcttaacc agccgcttac
    37381 aggccactga agcccggttg ccagtacttg ccgaagagat gtcattactg atgctggaca
    37441 gccgccgggt gatccaaagc attcagttga tgaaatcgct gggcggcggg tatcaggcgg
    37501 ctcccgtcgt cgagaaaaaa taaaatgtct gccgcgtgat ggctgtcacg cggtatttcg
    37561 tttcgtcacg tcaaaactga cgacagcctg tttttcgtca gagttttgaa taaatagtgc
    37621 ccgtaatatc agggaatgac cccaaataaa atgtggcata aaagatgcat actgtagtcg
    37681 agagcgcgta tgcgtgattt gattaactgg agcgagaccg atgaaaaaag tcgtcacggt
    37741 ttgcccctat tgcgcatcag gttgcaaaat caacctggtc gtcgataacg gcaaaatcgt
    37801 ccgggcggag gcagcgcagg ggaaaaccaa ccagggtacc ctgtgtctga agggttatta
    37861 tggctgggat ttcattaacg atacccagat cctgaccccg cgcctgaaaa cccccatgat
    37921 ccgtcgccag cgtggcggca aactcgaacc tgtttcctgg gatgaggcac tgaattacgt
    37981 tgccgagcgc ctgagcgcca tcaaagagaa gtacggtccg gatgccatcc agacgaccgg
    38041 ctcctcgcgt ggtacgggta acgaaaccaa ctatgtaatg caaaaatttg cgcgcgccgt
    38101 tattggtacc aataacgttg actgctgcgc tcgtgtctga cacggcccat cggttgcagg
    38161 tctgcaccaa tcggtcggta atggcgcaat gagcaatgct attaacgaaa ttgataatac
    38221 cgatttagtg ttcgttttcg ggtacaaccc ggctgattcc cacccaatcg tggcgaatca
    38281 cgtaattaac gctaaacgta acggggcgaa aattatcgtc tgcgatccgc gcaaaattga
    38341 aaccgcgcgc attgctgaca tgcacattgc actgaaaaac ggctcgaaca tcgcactgtt
    38401 gaatgcgatg ggccatgtca ttattgaaga aaatctgtac gacaaagcgt tcgtcgcttc
    38461 ccgtacagaa ggctttgaag agtatcgtaa aatcgttgaa ggctacacgc cggagtcggt
    38521 tgaagatatc accggcgtca gcgccagtga gattcgtcag gcggcacgga tgtatgccca
    38581 ggcgaaaagc gccgccatcc tgtggggcat gggtgtaacc cagttctacc agggcgtgga
    38641 aaccgtgcgt tctctgacca gcctcgcgat gctgactggt aacctcggta agccgcatgc
    38701 gggtgtaaac ccggttcgtg gtcagaacaa cgtgcagggt gcctgcgata tgggcgcgct
    38761 gccggatacg tatccgggat accagtacgt gaaagatccg gctaaccgcg agaaattcgc
    38821 caaagcctgg ggcgtggaaa gcctgcctgc tcataccggt tatcgcatca gcgagctgcc
    38881 gcaccgcgca gcgcatggcg aagtgcgtgc cgcgtacatt atgggcgaag atccgctgca
    38941 aactgacgcg gagctgtcgg cagtacgtaa agcctttgaa gatctggaac tggttatcgt
    39001 tcaggacatc tttatgacca aaaccgcgtc ggcggcggat gtcattttgc cgtcaacgtc
    39061 gtggggcgag catgaaggcg tgtttactgc ggctgaccgt ggcttccagc gtttcttcaa
    39121 agcagttgaa ccgaaatggg atctgaaaac ggactggcaa atcatcagtg aaatcgccac
    39181 ccgtatgggt tatccgatgc actacaacaa cacccaggag atctgggatg agttgcgtca
    39241 tctgtgcccg gatttctacg gtgcgactta cgagaaaatg ggcgaactgg gcttcattca
    39301 gtggccttgc cgcgatactt cagatgccga tcaggggact tcttatctgt ttaaagagaa
    39361 gtttgatacc ccgaacggtc tggcgcagtt cttcacctgc gactgggtag cgccaatcga
    39421 caaactcacc gacgagtacc cgatggtact gtcaacggtg cgtgaagttg gtcactactc
    39481 ttgccgttcg atgaccggta actgtgcggc actggcggcg ctggctgatg aacctggcta
    39541 cgcacaaatc aataccgaag acgccaaacg tctgggtatt gaagatgagg cattggtttg
    39601 ggtgcactcg cgtaaaggca aaattatcac ccgtgcgcag gtcagcgatc gtccgaacaa
    39661 aggggcgatt tacatgacct accagtggtg gattggtgcc tgtaacgagc tggttaccga
    39721 aaacttaagc ccgattacga aaacgccgga atacaaatac tgcgccgtgc gcgtcgagcc
    39781 gatcgccgat cagcgcgccg ccgagcagta cgtgattgac gagtacaaca agttgaaaac
    39841 tcgcctgcgc gaagcggcac tggcgtaata cagtcctttc tacagcctcc tttcggaggc
    39901 tgttttttta tccattcgaa ctctttatac tggttactcc ctacccaatc gtattatcaa
    39961 aatgaaaaaa attatcgcat tgatgttgtt tttgacattc tttgcccacg ccaacgactc
    40021 cgagcctggc agccagtatt taaaggcagc agaggccggg gaccgacgcg cacaatattt
    40081 tcttgccgac agctggttta gctccggcga tttgagcaaa gccgaatatt gggcacagaa
    40141 agccgccgac agcggtgatg ctgatgcctg cgcgctgctg gcgcagatca aaatcaccaa
    40201 tccggtcagt ctggactatc cacaagcaaa agttcttgca gagaaagcgg cgcaagcggg
    40261 cagtaaagaa ggtgaagtaa cgctggcgca tattctggta aatactcaag cgggtaaacc
    40321 ggattatcca aaggctattt cgctgttaga aaacgcctcg gaagatctgg agaacgactc
    40381 tgccgtcgat gcccaaatgc tgcttggttt gatttacgcc aacggcgtgg gcattaaggc
    40441 cgacgatgac aaggcaacct ggtatttcaa acgcagctct gcaatttccc gaaccggtta
    40501 ttccgagtac tgggcgggaa tgatgttctt aaacggtgaa gaaggtttta tcgagaagaa
    40561 caagcaaaag gcgctgcact ggttgaacct gagctgtatg gaagggtttg ataccgggtg
    40621 tgaagagttt gaaaaattaa ccaacggtta aggtaggcct gataagacgc ggcaagcgtc
    40681 gcatcaggca ttcacacatt gccgggtgga tatccccggc aatcttcaat tattgatccg
    40741 cagttttatc aaatttgccc agcacttcac gctcataagc cagcgccttt ttgcggtcaa
    40801 atttgtgttc ccacttggca atcaccagca ccgccagcgc attacccacc acgttcagcg
    40861 cagtacgcgc catgtcgagg atacggtcaa caccagcaat aaacgccaga ccttccagcg
    40921 gaatacctac gctacccagc gttgccagca gcaccacaaa cgacacgccc ggcacgccag
    40981 caatcccttt cgaggtcacc atcagcgtca gcaccagaat gatttcctgc cagatggaca
    41041 ggtcaatgcc atacagctga gcgatgaaaa tagcggcaat actttgatac agcgtcgaac
    41101 catcaaggtt aaaagagtaa ccggtcggca ccacgaagct ggtgatcgac gccggtgctc
    41161 cgtaggcttc catcttctca ataattcgcg gcagtacgct ttcagagctg gcagtggagt
    41221 acgccagaat cagctcatct ttcagaatac gaatcaggat ccagacgctt aacccgcaca
    41281 ggcgcgccac aattcccagc actaccagcg cgaagaacag aatggcgaaa tgcaccagca
    41341 gcaccagttt cgccagtggc cacagagaag agaaaccaaa gttagccacc gtcaccgcaa
    41401 tcagcgcaaa cacacccacc ggtgcataac gcatcaccat gtgagtcact ttaaacatgg
    41461 tttcagagat ggagcggaac acggtcacca gcagttcacg atgcgtcgcg ggcagggaag
    41521 aaagccccag accaaacagc accgagaaaa agatgatcgg cagcatttcg cctttcgcca
    41581 tcgacgccac aatgttcgtc ggcaccagcg acaaaatcgt gcccataatg ccgtgggaac
    41641 tgctttgtac cgcttccgta gtgctctgat atttcgagat atcgacggtc gccagctgcg
    41701 acatatccac cccggcaccg ggctggaaga cgttcgccag agtgatcccc aaaatgatgg
    41761 cgacggtggt gatcacctcg aagtaaataa tggttttcgc gccaatacgc ccgagctgtt
    41821 tggcatcacc aacacccgcg atacccacca ccagcgtgga gatcacaatc ggcacaacaa
    41881 tcattttgat cagatggatg aagatatcac ccgccggaga gagcaaattg acgaccagcc
    41941 agtcgcggct gtcgctatgg tagtgcaggt agcttcccag gagaatgccc agcaccatag
    42001 caaacagaat ctgccaggcc aggctgaatt ttatattttt catcagaata acttcctcaa
    42061 tggagcgccg ttccccgttc agaaacgcga gaacgtgaca tactgccagg gcatgaattg
    42121 tgttgcgttg gtttgtagat ttttttaacg cttatgatca gtatcatttg cgcggtatgt
    42181 tgcgcaaccc tttaacaaca gagcttttcg ctgcattaat gcataataac gcgagtttat
    42241 ggcaggatta atgaataacc atttgttatg gaaagatttt tatttgttta atctgcaata
    42301 atactgatta gtgattattt ttggcatatg ttcattcgat tattttttat gcagctcaat
    42361 gacataatct tcatcaccag atgattaatc cgatctccgc tgcaacagtt ttgccatatt
    42421 aatcgactcg accagctgtg tccggttgat ccggggtgag ttgagatcca tcactttttg
    42481 ccatagttcg atggcttgcg cgtaattcgc ctgcataaac gcatcggaag ccagcagcat
    42541 cagagcggtg atttcgttag agtccagcgc gagggctttg tcgatcattg cgcaagtctg
    42601 ggcggtcata tgctggctgg cctggtaata aagcaccgtc gccagcgccg catacagttc
    42661 agcgttctca ccacgtagtt gcaacgcctg acgatacgcc agcagcgaat tgctgtaatc
    42721 gttttgccac agatagtact cgcccagtaa cgcccattgt tcgctgtttt gtggattagc
    42781 gcggatttta tcctgcaatg cctgaagctg cgcttccggg gtttgctggc tggcaaactg
    42841 atgtagcgga tcgcgctgac gctgatactc cgcacgtacc gcctgccatt ttgggcttaa
    42901 cagataactg ccgacacaga ggaataccat caatatcgtg agtgttgtca gcattttcac
    42961 cggtatttga ggctgtttca tcgcttagcc ctcactcgcc agaggatcag tgccatcagc
    43021 aataacaaca ccactggcag cgcccacaac accagcgtct gacccgttaa cggtgggtta
    43081 tagcgaacaa aatctccgta gcgttcggtc atccagccaa tgatttcgac ttcgttcttc
    43141 ccctccgaca ccatgctata aacctgatgg cgcatactga ccgccaccgg cgcattggat
    43201 tcgagtaagt tttgattctg gcactgcgga caacgtaact ggctggcaat atttaacgcc
    43261 tgttgctgtt gttgcggatt ggcgaattgc caggtgtcta cgacctgagc gcgcgcaaaa
    43321 caggtaaata acagcagtag cgtgagaagc cctttattca tcgcgcttct tcccccgcca
    43381 tccgcttaac aatgcgcccg caatcatcaa caatcctcca ccccagatcc agcgcacacc
    43441 gctttgtaca tacaaacgaa aagcgtaacg atccgctccg gttttttcac ccatgaccgc
    43501 ataccagtca tggatgccgt tccagcgaat tgacggttcc atcatttgct gacggcgtgc
    43561 ttcataaaaa cgccgctccg gcgttaattc accaatgcgt tgctgatggt caaacagcgc
    43621 cactatcgct ttttcgctgg tgtaattgcc tttggcttgc agatcgaggc gctcaaaacg
    43681 gaaggtgtat cctgccagcg tcacctgctg acccggctgt aaattcaggc tgatctcctg
    43741 acggctgacg ctggaaacca cgatccctgc ggcaaataac agcacgcccg catgagcgac
    43801 cagcgccgga agctgcgcgc gtttgccaga gacaaacgtc gccagcacaa tcaccaccag
    43861 catcaacaga ccaaacggta acgtcgcgcg gttaaaatac ggcgcgccaa cggagaggcg
    43921 tccccagccc agcagaccgt aaatcatcgg ataaagcgtt cccaccagca cgatcagtag
    43981 cactgcgcaa aacagcaaca gcgtagcgag gattaacatt tcccgcgata accccgaaaa
    44041 acgcaccgcc gggccaccgt cccgcgctcg ccagccatac agagccaaag acgcaaggct
    44101 aatcagtgca aacaggctga acaacggcac ggcgcggacg ttatccagtg cgaacgcatg
    44161 aaccgaaacc agaatgccag aacggacaat taaggtgccc agcagcgaca gcatcagagt
    44221 aactatcgcc agcaacagcg accagtggca aaaaatcccc cgctggcgtg tcagggataa
    44281 actgtgcagc agcgcagtgg cagaaagcca gggtaataaa gaggcatttt ccaccggatc
    44341 ccagaaccac cagccgcccc agccgagttc gcagtaggcc caccaggaac cgaggatgat
    44401 ccccgccgtt aatgcactcc agccaggtaa cgcccagcgc cagcaaattc gggcgctggc
    44461 cgcatcaaac tcgccgcgca gtagactcgc cagcgccacg ctcgccgcta ccatcaaacc
    44521 gccatagcca aggtaaagca gcggtggatg aaagataaga ccgggatgtt gcagcatcgg
    44581 attgagatcg cggccttcga ttgctggtgg aaatatccgc acgaagggat cggaccacag
    44641 caccacaaac agtagcagtg cggcgagcat gagagataaa acggctaacg tcagcggaaa
    44701 tagcggatcg gtttgctgcc gataatgcca ggcaaacagc gcgctccagg cggaaagcag
    44761 cagcacccaa agcagcagcg aaccttcatg gccgccccac accgctgcca gcttgaattc
    44821 ccacgacagc aggctatagc tatgttgcgc gacataaatg accgagaaat cgctgatgag
    44881 aaaacaatac gtcagtacgc caaaggcgag cagcaggagc gcgaactgcg ccagaatgcc
    44941 gatgcaagtg agtcgcatca tggcaggcca gcgcaaccgc actcccgcaa aggccgtcaa
    45001 cggggtcaac acgttgaccc cgagacttaa caacaacgcc agaaaaccga cttcaggaag
    45061 gaatacgtcc agaaaccacc tcttttctgg cttacgccac cgtcaactgt ccggcgtaga
    45121 gaatgaaaaa gcgcagcatc aacacgccga ccagactggc cccacacgcc gccagcacgg
    45181 caggaatgcc ggaactgcga ttgacccacg gtttgagcaa cattggcaca atcagcccca
    45241 gcccggcgac accaagccag aaccaccacg tccagaaacc gccacctaat gccgccacca
    45301 gcgcacgaac tttaccgtca tcgcccagcg ccagaccgac aaaaaacgcc accagcagga
    45361 agatttcacc ccataccacg gggatttcca tccggtgaac aaagtgcgct tccgtggaat
    45421 gcgggttact gcgttggcgt atcgccatgg cgatcagcgc caccgccgca ccggacgaga
    45481 tgccggagaa gaggaacagc accggcagga tcgggttatt gaggaacgga tacgatttca
    45541 gcgccgacag cagaaagccg gtataagccc ccagcagcac cgccaacacc agcatcagcg
    45601 tttccagtcc gcgatgaacg ggcgtcagca ggctcagaac cttttgcacg atccccagct
    45661 tcggcaacca gcgttgttgc agggcaagca aatcatgttc aaagattttc gccagccaca
    45721 gcaccagcac caccatgtag agctgaaaca gcatcacccc catcgacatc accgaggtaa
    45781 aactgtagtg gaacatcagc ttccagaagg tccacggtct tgtcaggtgg aagacgagga
    45841 tcaacagacc gaggatcacc gcgcccggcc cgacaatcag cgtggtgcgc agcaacgtac
    45901 tgtctgcacc gcccgcctgc gggtagaagc gacgtaacag cacggccagc gtcaccagac
    45961 cggcagaaat accaatcaaa aacaggtaga tggcaatcgg ccagtcccac accagcgatt
    46021 caaaatgaaa tgcggaagtc tgcgtcattg gctcacctcc ccgtatttaa acggtacgcg
    46081 gtacagcttc ggtttggttc ccagcgccag cttgtagcgg taagtaggct tctggcgcag
    46141 cagttgcgaa atctcactgt taggatcgtc cagattgcca aacgtcagcg ccttcgtcgg
    46201 gcaagcctca acacacgcgg gcagcttacc ggcctgcaaa ttggttttac ggcagaaatc
    46261 gcatttgtcc gccgtcttcg tgaccggatg gataaagcgc acgcggtacg gacaggcggc
    46321 gatgcagtac tgacaaccga cgcagagatc aggattaaca tcgacaatcc cgctagcggc
    46381 atcacgaaaa gacgcaccgg tcgggcagac gtcaacgcac ggcgcatgat cgcagtgctg
    46441 gcaagactta cggaagaagc gatatttcac atcaggaaat tcgccctgcg gctcgctacg
    46501 aataatcgtc aggcgcgaga cgccttccgg caccttgttc acttcccgac aagcatccat
    46561 acaggcggta cagccgatgc ataacgactc gtcatgcacc atgccgtaac gcaccccatt
    46621 gatattcaac gttttcgcca ccacgcgccc tgcggtgccg ctgacggctg ccagcacgcc
    46681 gacgccggtg agaaactggc gacgagacca ggtcatggct gctccttaag caacggaaca
    46741 gacgccgggt taaagttcgg attggtgcgc tgatcgctgt ggcaatcgac gcaaatctta
    46801 atccgtcctt tgtcacttaa cgtctgcatc gtatcttgct gtggatgcag ggaatggcag
    46861 ctggcgcacg ccactttggt tacgtggaca tcgtgcggcc agaacgcttt ttgcaactgt
    46921 tccggcagat gacaggacat acagacgctg ttctgctctc caaccttgta catcggctcg
    46981 ttaaagcgca tcacatcttt caccccttcg cggtgttgcg gtgatggctg gccgtgacag
    47041 ttggtgcagg tgaccggcag tttgttattc gggttgataa cggaagcatg tttgccatgc
    47101 ataccttcgg tatctggttt atgacagtcc agacaggcgg catccgggtt acgctgctgg
    47161 gtaacttcgt agcgatcatc agatgcctgc gccgcagggg tagcggtaat tccgttcagg
    47221 ctccacaaca ggcccgacgc cagcaccccg gcagttaata acgaacgtaa tacgctcata
    47281 ttcactccat tgtgttttcc gccccttgcg aggcggaacg gggttattgg cttaacagac
    47341 cgtttttacg tgcctgttct tcccactgcg ggatcaccgt tttgatgaag tcctgcttct
    47401 cggccttgat ttgttccatg ttcaggccaa tggcctgctg ggctttctct ttggttgaga
    47461 tatccgggat ctggatttca tgggtgatgc ctttggtcgc cagcaggcgc gccagtttgg
    47521 tgcgtgcatc cgccgcttta tccatcgccg taccgagcat ccgtaaacct tcttccggtg
    47581 cgtgcatatg aatgccgtgg gaagcgatcg ccagatccca gcgccactgg gcatgacgga
    47641 tatcgtcctg aattggcttc atttcagctt ccgtcgcgcc tgcatccagc gctgctttcg
    47701 cttcgaagtg agcgtgaacc agttgatctt caaccttgat tttcaggtcg ttaatcgact
    47761 gcttacgttc cgcgaccact ttttgcaagg cagctttgtc ctgggtatgg cagttcgcac
    47821 aagtctgggc gaagttatca aacggattac caattttatg gtcggtatag agtttgcctt
    47881 cggcgttctg cacttttggc atatggcagt cgatacaggt cacgttgttt ttaccgtgaa
    47941 tgcccgctgt ccaggtttca tattccgggt gctgcgcttt cagcattggc gttttcgaca
    48001 gggagttagt ccagtcagag aaggcaattt tgtcgtaata ctgctccata ttttcgactt
    48061 tcatgccgtc atcccacggg aatttaaccg ctttgttttt gccgtcgaag taatactcca
    48121 catggcactg accgcaaacc atcgattgct ggtcgaaacg tccggctttc tcaaaaggtt
    48181 taccaatggc ttccatcgcg cgagccgcat acggacggga aagggttaac tccggtttgc
    48241 ctttggcgaa ctctggtgag gcggtgttat ggcagtcggc acaacctaag ttgttgacga
    48301 tttccgggcc gccgcgcgcc cacttaccgt ggaagtagcc atcttcgccg tctttctgga
    48361 tcagacgcgc cacatccggg cttttacaac tccagcacgc catcggtagc ggaccatctt
    48421 cagcgttttt cggcgcaccg gtacgcaggg tttcacgcac atcggtcaca gcaaaggcat
    48481 gtccacgcgg cttgttgtaa tcgcgcgaga agggataccc cgcccacagg atcaccagcc
    48541 gtggatcttc cgccagggcg tcaacacgct ctgactgttc cgaggtggct ttccaggaga
    48601 gatattgatc gggatgctgc ggggcaaagg tttcattctt cgcttccaca gttacaggtt
    48661 ttgcgggagc tgccgtttgt tcagcgtgaa cagaagtgaa aaagaaaaaa ggaatcaata
    48721 agctgaagat acggcgtgcg tttattttta tccttgtcat aggggcttca tccgaattgc
    48781 gccattgtta caatggcggt ttttattgtt tttcacgaca gtaaccgcac ctacactgtc
    48841 atgacattgc tcgcccctat gtgtaacaaa taaccacact gtgaatgttg tctttaatca
    48901 attgtaagtg catgtaaaat accactttag agttagttag tatcttcctc tttttcaaca
    48961 gcaatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttgctg accttcaaaa
    49021 attaccctgc cgtttatttg cacaattcta cttttgcgtg atctgtcgcc caaatactaa
    49081 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa caccctacaa
    49141 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    49201 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    49261 taccttctgg ggcgaacagg gaaaaattct tgactggatc aaaccttacc agaaggtgaa
    49321 aaacacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    49381 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgta ccgccatcat
    49441 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    49501 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    49561 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    49621 cgcggtgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    49681 tgattccaac tcacgactgg tgatcacttc cgacgaaggt gtgcgtgccg ggcgcagtat
    49741 tccgctgaag aaaaacgttg atgacgcgct gaaaaacccg aacgtcacca gcgtagagca
    49801 tgtggtggta ctgaagcgta ctggcgggaa aattgactgg caggaagggc gcgacctgtg
    49861 gtggcacgac ctggttgagc aagcgagcga tcagcaccag gcggaaaaga tgaacgccga
    49921 agatccgctg tttattctct acacctccgg ttctaccggt aagccaaaag gtgtgctgca
    49981 tactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    50041 tccgggtgat atctactggt gcaccgccga tgtgggctgg gtgaccggac acagttactt
    50101 gctgtacggc ccgctggcct gcggtgcgac cacgctgatg tttgaaggcg tacccaactg
    50161 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    50221 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgatcg agggtaccga
    50281 ccgttcgtcg ctgcgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    50341 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    50401 cgaaactggc ggtttcatga tcacgccgct gcctggcgct accgagctga aagccggttc
    50461 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    50521 ggaaggcgct accgaaggca gcctggtgat caccgactcc tggccgggtc aggcgcgtac
    50581 gctgtttggc gatcacgaac gttttgaaca gacctacttc tccaccttca aaaatatgta
    50641 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    50701 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagattg agtcggcgct
    50761 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    50821 tcaggcgatc tacgcctacg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    50881 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    50941 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    51001 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    51061 cgtagtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    51121 tgccggatgc gagtcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    51181 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    51241 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    51301 gagaatctgt gatgaatggc actatttatc agcggataga agacaatgcg catttcaggg
    51361 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    51421 atatcggctt tattttactg atcgccttcg cgcccggctg gctggcacgc cgctgaatcc
    51481 gaacaccagc gtcacacgcg gtattccaat tggtgttgga gtgattgtga tctcctttgt
    51541 tctcaccggt atctacatct ggcgggcgaa cggcgaattc gaccgtctta ataatgaagt
    51601 cctgcatgag gtacaagcat catgaaaaga gttctgacgg cgcttgccgc cacactccct
    51661 ttcgcagcta acgccgcgga tgctattagc ggggccgtag agcgccagcc aacgaactgg
    51721 caggcgatta ttatgttcct gattttcgtc gtgtttacgc tcggcattac ctactgggca
    51781 tcaaaactcg tacgttctcg tagcgactac tacaccgcag gcggcaatat cactggcttc
    51841 cagaacgggc tggcgattgc cggggactat atgtccgccg cctcattctt ggggatctcc
    51901 gcgctggtgt ttacctccgg ctatgacggc ttaatttact cgctgggctt cctggtgggc
    51961 tggccgatca ttttgttcct gattgccgaa cgtctgcgta acctggggcg ctacaccttt
    52021 gccgatgtgg cctcttaccg tctgaaacaa gggccgattc gtattctttc ggcctgtggt
    52081 tctctggtgg tggtggcgct ttaccttatc gcccagatgg tgggcgcagg taaactgatc
    52141 gagctgctgt ttggccttaa ctatcacatt gcggtggtgc tggtcggcgt gctgatgatg
    52201 atgtacgtcc tgttcggcgg catgctggcg accacttggg tacagattat caaagccgtg
    52261 ctgttgctgt tcggtgccag ctttatggcc tttatggtga tgaaacacgt cggctttagc
    52321 ttcaacaatc tgttcagtga agcgatggcg gtacacccga aaggtgtcga catcatgaag
    52381 ccgggcgggc tggtgaaaga tccgatctcc gcactctctc tgggtctggg actgatgttt
    52441 ggtacggcgg gcttgccgca cattctgatg cgcttcttta cagtcagcga tgcccgcgaa
    52501 gcacgtaaga gcgtgttcta cgccaccggg tttatgggct acttctatat tctgaccttt
    52561 attatcggct tcggcgcgat catgctggtt ggtgcgaatc cggaatataa agacgcggcg
    52621 ggccatctga ttggtggtaa caacatggcg gccgttcacc tggcgaatgc agtgggcggc
    52681 aacctgttcc tcggttttat ttcagcggtt gctttcgcca ctatcctcgc ggtggttgcg
    52741 ggtctgacgc tggcgggcgc atccgcggtt tcgcatgact tgtacgctaa cgtcttcaaa
    52801 aaaggcgcga ccgaacgtga agagctgcgg gtatcaaaaa tcaccgtact gatcctcggc
    52861 gtgattgcga ttatcctcgg cgtgctgttt gagaatcaga acatcgcctt tatggtgggg
    52921 ctggcgtttg ccatcgcggc gagctgtaac ttcccgatca ttctgctttc tatgtactgg
    52981 tcgaaactga ccacgcgtgg cgcgatgatg ggtggctggc tggggctgat taccgcagta
    53041 gtactgatga tcctcggccc gacgatttgg gtacagatcc ttggtcacga aaaagccatc
    53101 ttcccgtatg aatacccggc gctgttctct atcaccgtgg cattcctcgg catctggttc
    53161 ttctcggcaa ccgataactc agcggaaggg gcgcgcgagc gtgaactgtt ccgcgcgcag
    53221 tttatccgct cccagaccgg ctttggcgtt gagcaaggcc gcgcacatta atctctcccc
    53281 ttccccggtc gcctgaccgg ggaatactct tcctctccag catgcatcac cttttcccaa
    53341 aatattaaac aaataaactc attaaaaaat gagcgatttt tgacagtcgt agaaaatgat
    53401 a
//
LOCUS       DAQSWQ010000018        53031 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.018, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000018 DAQSWQ010000000
VERSION     DAQSWQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53031
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.018"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="R8G18_001092"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="R8G18_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HEE9794859.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="R8G18_001093"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="R8G18_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEE9794860.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="R8G18_001094"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="R8G18_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEE9794861.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="R8G18_001095"
     CDS             4216..4620
                     /locus_tag="R8G18_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HEE9794862.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="R8G18_001096"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="R8G18_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HEE9794863.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5509..6075
                     /gene="satP"
                     /locus_tag="R8G18_001097"
     CDS             5509..6075
                     /gene="satP"
                     /locus_tag="R8G18_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEE9794864.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6110..6697)
                     /gene="mog"
                     /locus_tag="R8G18_001098"
     CDS             complement(6110..6697)
                     /gene="mog"
                     /locus_tag="R8G18_001098"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEE9794865.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6812..7765)
                     /gene="tal"
                     /locus_tag="R8G18_001099"
     CDS             complement(6812..7765)
                     /gene="tal"
                     /locus_tag="R8G18_001099"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEE9794866.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8044..9473
                     /locus_tag="R8G18_001100"
                     /pseudo
     CDS             8044..9473
                     /locus_tag="R8G18_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            9543..10319
                     /gene="yaaA"
                     /locus_tag="R8G18_001101"
     CDS             9543..10319
                     /gene="yaaA"
                     /locus_tag="R8G18_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEE9794867.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10472..10768)
                     /locus_tag="R8G18_001102"
     CDS             complement(10472..10768)
                     /locus_tag="R8G18_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HEE9794868.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10982..12268)
                     /gene="thrC"
                     /locus_tag="R8G18_001103"
     CDS             complement(10982..12268)
                     /gene="thrC"
                     /locus_tag="R8G18_001103"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEE9794869.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12269..13201)
                     /gene="thrB"
                     /locus_tag="R8G18_001104"
     CDS             complement(12269..13201)
                     /gene="thrB"
                     /locus_tag="R8G18_001104"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEE9794870.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13203..15665)
                     /gene="thrA"
                     /locus_tag="R8G18_001105"
     CDS             complement(13203..15665)
                     /gene="thrA"
                     /locus_tag="R8G18_001105"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEE9794871.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15692..15811)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15747..15812)
                     /gene="thrL"
                     /locus_tag="R8G18_001106"
     CDS             complement(15747..15812)
                     /gene="thrL"
                     /locus_tag="R8G18_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HEE9794872.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16026..16712)
                     /locus_tag="R8G18_001107"
     CDS             complement(16026..16712)
                     /locus_tag="R8G18_001107"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HEE9794873.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17112..17252)
                     /locus_tag="R8G18_001108"
     CDS             complement(17112..17252)
                     /locus_tag="R8G18_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794874.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17348..18064
                     /gene="arcA"
                     /locus_tag="R8G18_001109"
     CDS             17348..18064
                     /gene="arcA"
                     /locus_tag="R8G18_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HEE9794875.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18124..19476)
                     /gene="creD"
                     /locus_tag="R8G18_001110"
     CDS             complement(18124..19476)
                     /gene="creD"
                     /locus_tag="R8G18_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEE9794876.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19534..20958)
                     /gene="creC"
                     /locus_tag="R8G18_001111"
     CDS             complement(19534..20958)
                     /gene="creC"
                     /locus_tag="R8G18_001111"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HEE9794877.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20958..21647)
                     /gene="creB"
                     /locus_tag="R8G18_001112"
     CDS             complement(20958..21647)
                     /gene="creB"
                     /locus_tag="R8G18_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HEE9794878.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21660..22133)
                     /gene="creA"
                     /locus_tag="R8G18_001113"
     CDS             complement(21660..22133)
                     /gene="creA"
                     /locus_tag="R8G18_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HEE9794879.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22344..23213
                     /gene="robA"
                     /locus_tag="R8G18_001114"
     CDS             22344..23213
                     /gene="robA"
                     /locus_tag="R8G18_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HEE9794880.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23210..23857)
                     /gene="gpmB"
                     /locus_tag="R8G18_001115"
     CDS             complement(23210..23857)
                     /gene="gpmB"
                     /locus_tag="R8G18_001115"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HEE9794881.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23909..24430
                     /gene="yjjX"
                     /locus_tag="R8G18_001116"
     CDS             23909..24430
                     /gene="yjjX"
                     /locus_tag="R8G18_001116"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HEE9794882.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24515..24841)
                     /gene="trpR"
                     /locus_tag="R8G18_001117"
     CDS             complement(24515..24841)
                     /gene="trpR"
                     /locus_tag="R8G18_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HEE9794883.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24931..26868)
                     /gene="sltY"
                     /locus_tag="R8G18_001118"
     CDS             complement(24931..26868)
                     /gene="sltY"
                     /locus_tag="R8G18_001118"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HEE9794884.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27079..28746
                     /gene="ettA"
                     /locus_tag="R8G18_001119"
     CDS             27079..28746
                     /gene="ettA"
                     /locus_tag="R8G18_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEE9794885.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(29053..30285)
                     /gene="nadR"
                     /locus_tag="R8G18_001120"
     CDS             complement(29053..30285)
                     /gene="nadR"
                     /locus_tag="R8G18_001120"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HEE9794886.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30306..31688)
                     /gene="radA"
                     /locus_tag="R8G18_001121"
     CDS             complement(30306..31688)
                     /gene="radA"
                     /locus_tag="R8G18_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEE9794887.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31737..32705)
                     /gene="serB"
                     /locus_tag="R8G18_001122"
     CDS             complement(31737..32705)
                     /gene="serB"
                     /locus_tag="R8G18_001122"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HEE9794888.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32811..33455
                     /locus_tag="R8G18_001123"
     CDS             32811..33455
                     /locus_tag="R8G18_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HEE9794889.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33483..34499
                     /gene="lplA"
                     /locus_tag="R8G18_001124"
     CDS             33483..34499
                     /gene="lplA"
                     /locus_tag="R8G18_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HEE9794890.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34531..34680
                     /locus_tag="R8G18_001125"
     CDS             34531..34680
                     /locus_tag="R8G18_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794891.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(34955..35674)
                     /gene="deoD"
                     /locus_tag="R8G18_001126"
     CDS             complement(34955..35674)
                     /gene="deoD"
                     /locus_tag="R8G18_001126"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEE9794892.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35754..36977)
                     /gene="deoB"
                     /locus_tag="R8G18_001127"
     CDS             complement(35754..36977)
                     /gene="deoB"
                     /locus_tag="R8G18_001127"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEE9794893.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37029..38351)
                     /gene="deoA"
                     /locus_tag="R8G18_001128"
     CDS             complement(37029..38351)
                     /gene="deoA"
                     /locus_tag="R8G18_001128"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEE9794894.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38478..39257)
                     /gene="deoC"
                     /locus_tag="R8G18_001129"
     CDS             complement(38478..39257)
                     /gene="deoC"
                     /locus_tag="R8G18_001129"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEE9794895.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39515..41065
                     /locus_tag="R8G18_001130"
     CDS             39515..41065
                     /locus_tag="R8G18_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HEE9794896.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41037..41900
                     /locus_tag="R8G18_001131"
     CDS             41037..41900
                     /locus_tag="R8G18_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HEE9794897.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(42315..43097)
                     /locus_tag="R8G18_001132"
     CDS             complement(42315..43097)
                     /locus_tag="R8G18_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEE9794898.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(43094..44167)
                     /locus_tag="R8G18_001133"
     CDS             complement(43094..44167)
                     /locus_tag="R8G18_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEE9794899.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(44289..44450)
                     /locus_tag="R8G18_001134"
     CDS             complement(44289..44450)
                     /locus_tag="R8G18_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEE9794900.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44577..45182)
                     /gene="osmY"
                     /locus_tag="R8G18_001135"
     CDS             complement(44577..45182)
                     /gene="osmY"
                     /locus_tag="R8G18_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HEE9794901.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45575..47164)
                     /gene="prfC"
                     /locus_tag="R8G18_001136"
     CDS             complement(45575..47164)
                     /gene="prfC"
                     /locus_tag="R8G18_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEE9794902.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(47255..47932)
                     /gene="yjjG"
                     /locus_tag="R8G18_001137"
     CDS             complement(47255..47932)
                     /gene="yjjG"
                     /locus_tag="R8G18_001137"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEE9794903.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47947..48393)
                     /gene="rimI"
                     /locus_tag="R8G18_001138"
     CDS             complement(47947..48393)
                     /gene="rimI"
                     /locus_tag="R8G18_001138"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEE9794904.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(48362..48775)
                     /gene="holD"
                     /locus_tag="R8G18_001139"
     CDS             complement(48362..48775)
                     /gene="holD"
                     /locus_tag="R8G18_001139"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HEE9794905.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48878..49909
                     /gene="rsmC"
                     /locus_tag="R8G18_001140"
     CDS             48878..49909
                     /gene="rsmC"
                     /locus_tag="R8G18_001140"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HEE9794906.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            50177..50263
                     /locus_tag="R8G18_001141"
     tRNA            50177..50263
                     /locus_tag="R8G18_001141"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50211..50213,aa:Leu,seq:cag)
     gene            50292..50378
                     /locus_tag="R8G18_001142"
     tRNA            50292..50378
                     /locus_tag="R8G18_001142"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50326..50328,aa:Leu,seq:cag)
     gene            50413..50499
                     /locus_tag="R8G18_001143"
     tRNA            50413..50499
                     /locus_tag="R8G18_001143"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50447..50449,aa:Leu,seq:cag)
     gene            complement(50538..50774)
                     /locus_tag="R8G18_001144"
     CDS             complement(50538..50774)
                     /locus_tag="R8G18_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HEE9794907.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            50915..51703
                     /gene="fhuF"
                     /locus_tag="R8G18_001145"
     CDS             50915..51703
                     /gene="fhuF"
                     /locus_tag="R8G18_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HEE9794908.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(51741..52418)
                     /gene="bglJ"
                     /locus_tag="R8G18_001146"
     CDS             complement(51741..52418)
                     /gene="bglJ"
                     /locus_tag="R8G18_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HEE9794909.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(52376..>53031)
                     /locus_tag="R8G18_001147"
     CDS             complement(52376..>53031)
                     /locus_tag="R8G18_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEE9794910.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        12551 a        14318 c        13327 g        12835 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcatttttc ccctcaaaag
     5401 atcgtagaca ctgccccact ggctgattat tatgccgcgc cctgaaaaca ctactgttat
     5461 tcagggaaat tatttcacca ttcattcgat gatgattttt gaggaattat gggcaacact
     5521 aagttggcta atccggcacc gctgggcctg atgggcttcg gcatgaccac cattctgctt
     5581 aacctgcaca acgtgggtta tttcgctctg gacggtatta ttcttgccat gggcattttc
     5641 tacggcggca tcgcgcaaat ttttgccggt ctgctggagt acaaaaaagg caacactttc
     5701 ggtttaaccg cattcacctc ttacggttct ttctggttga cgctggttgc gattctgctg
     5761 atgccgaaac tgggtctgac cgatgcgcca aatgcacagt tccttggtgt ctacctgggt
     5821 ctgtggggcg tatttacgct gtttatgttc ttcggcacgc tgaaaggcgc acgcgttctg
     5881 caattcgttt tctttagcct gactgtgctg tttgccctgc tggcgatcgg taacattgcc
     5941 ggtaacgccg caatcatcca ctttgccggc tggattggtc tgatctgtgg tgccagtgca
     6001 atctatctgg cgatgggtga agtactgaac gagcagtttg gtcgcaccgt tctgccgatt
     6061 ggtgaatccc actaattgtt ttgagatccc cccgctcggg gggatttttt tattcgctaa
     6121 cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac ttctggtgcc gtttcaacgt
     6181 atggcccttc cagcaactga atgcagtacg gtacgctggc aaaaataccg tgtaccacaa
     6241 cgttacccgc agcgtccttc acaccttcca gcgtctcttt aatagacttc ggctgaccgg
     6301 gtaagttaag gatcagcgcc tgtttgcgaa tcacaccaac ctgacgcgaa aggatcgcag
     6361 ttggtacaaa atgcaggctt atctggcgca tctgttcacc aaagccaggc atctcgcggt
     6421 ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg cgccgggcca gttccgcccg
     6481 tggtgagcac cagatggcaa ctcatttcat ccactagctc acacaacgtt tgctcgatga
     6541 tcgcctgctc atcggggatt aagcgggttt ccagttcaaa cggcgtggtt agcgccgatg
     6601 tcagccattc ttccagcgca gggatgcctt tatcctgata aacgccgctg gatgcgcgat
     6661 cagagatgga aactaagcca atacgtaaag tattcatcaa gatttttccg tttaaacagt
     6721 ctcgttaaac agaatgatac actgcgaagg gagtgacaga caggcttcag aagaggtagc
     6781 gtgaccgact tcccggtcac gctaagaatg attacagcag atcgccgatc attttttcca
     6841 gtttttcctg gtcaatagca aacttacgga taccttccgc cagtttatct actgccattg
     6901 gatcctggtt gtgctgccac aggaactcgg actcagtgat acgcgccgga cgcgctttca
     6961 cttcgccggt gtaagacagt ttacgttcga tagccccttc gctctcagcc agctctttca
     7021 gcagtgccgg tgcgatggta agacggtcgc agcctgccag ttccagaatt tcgccgatgt
     7081 tacggaagct tgcgcccata accacggttt cataaccgtg ctctttgtag tactggtaga
     7141 tttcagatac agaaaccacg cccggatctt ctgccggagc gtactctttc ttatcagtat
     7201 tcgctttgta ccagtcaaga atacggccaa caaatggcga gatcaggaac acgcctgctt
     7261 ccgcacaagc acgagcctga gcgaaggaga acagcagggt caggttacag ttgatgcctt
     7321 ctttttccag ctgttctgca gcacggatac cctgccaggt agaagccagt ttgatcagaa
     7381 tacgatcgtt gctaatacca gcatcgttgt agagtttgat caggcgtttt gctttcgcaa
     7441 ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc agttgagata cggcccggaa
     7501 ccagtttcag gatttccaga ccaatattta ctgccagttt gtcggtcgcg tccacgatct
     7561 gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac agcatcatca atcaacttac
     7621 ggtattccgg aatctgcgct gcgttaagaa tgagagaagg gttggttgtg gcatcctgcg
     7681 gttgatacag cttcattgcc gcgatgtccc cagtgtcggc cactacggtg gtgtactgac
     7741 gaagggaggt caatttgtcc gtcatgatag tatttctctt taaacagctt gttaggggga
     7801 tgtaaccggt ctgccctgat gatatcacga cgcaacgtca gcgcaaccgc cgacaagacg
     7861 gttatcgcca ggcgagactg tttcggattt ctgacactgc gttgatatcg cccgccattt
     7921 ttatacaaaa cctcatgtat gctacgcaga agttatcaag tacctcgtag cgtatatact
     7981 tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc gaccggcaat aagagggata
     8041 tgcatgccag attttttctc ctttattaac agcgtccttt ggggatcggt aatgatttac
     8101 ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg gatttgtgca gtttcgctac
     8161 atccgccagt ttggcaaaag tcttaaaaat agcattcatc cacagccagg cggtttaacc
     8221 tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg gtagcggcaa cctggccggc
     8281 gttgcgctgg ctattaccgc cggtggacct ggagccgtct tttggatgtg ggttgccgcg
     8341 tttatcggca tggcgacctc gtttgccgaa tgttcccttg cacaacttta taaagaacgt
     8401 gacgtcaatg gacagtttcg tggaggaccg gcatggtata tggcgcgcgg gctggggatg
     8461 cgttggatgg gcgttctgtt cgccgtcttt ttgctcatcg cctacggcat aattttcagc
     8521 ggagttcagg cgaacgccgt tgcccgagcc ctgagttttc ttttgatttt cccccgctgg
     8581 tgacaggcat tattctcgct gtctttgctc tgctggcaat cactcgcggt cttcatggcg
     8641 tcgcccggct catgcagggg tttgtcccgt tgatggcgat aatctgggtg ctgaccagca
     8701 tggtgatttg cgtaataaat atcgggcaac ttccccacgt catttggtct atttttgaga
     8761 gtgcttttgg ctggcaggaa gcggcaggcg gcgcggcggg atatacctta agccaggcga
     8821 ttactaacgg ttttcagcgc agtatgtttt ccaatgaggc gggaatgggt tcgacgccaa
     8881 acgcggcagc ggcagcggcg tcctggcctc cgcatccggc agcgcaaggg attgtccaga
     8941 tgattggcat ttttatcgac accctggtca tctgtacggc aagcgccatg ctgatattac
     9001 tggcgggtaa cgacacaacc tacatgccgc tggaaggtat tcagcttatc cagaaggcga
     9061 tgcgggtgct aatgggttcc tggggtgctg agtttgttac cctcgtggtt attctgtttg
     9121 ccttcagctc catcgttgcc aactacattt atgccgaaaa caatctcttc tttttacgcc
     9181 tgaacaaccc taaagcgatc tggtgtttgc ggatctgcac cttcgcaacg gtcatcggcg
     9241 gcaccttgct aagtcttccg ctgatgtggc aactggcaga tatcataatg gcctgcatgg
     9301 ctattaccaa tttgaccgcc attttactgc tctcgcctgt ggttcatacc attgccagtg
     9361 attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg
     9421 atatcggtcg ccagctttct ccggacgcgt gggatgatgt ttcgcaggag taatcacaac
     9481 tatcgatcaa ctcattctct ttttttgcta aagtcggcat aaatttcctg caaggactgg
     9541 atatgctgat tcttatttca cctgcgaaaa cgcttgatta ccaaagcccg ttgaccacca
     9601 cgcgctatac gctgccggag ctgttagaca attcccagca cttgatccat gaagcgcgga
     9661 aactgacgcc tccgcagatt agcacgctga tgcgcatcag cgacaaactg gcgggtatca
     9721 acgccgctcg ctttcatgac tggcagccag atttcacgcc ggagaatgcc cgccaggcta
     9781 ttttggcgtt taaaggtgat gtctacaccg gcttgcaggc cgaaactttc agcgaagacg
     9841 atttcgattt tgcccaacag catttgcgaa tgctttccgg cttgtatggc gtactgcgcc
     9901 cgctcgattt aatgcagcct tatcgtctgg aaatggggat ccgtcttgag aatgcccgag
     9961 ggaaagatct gtatcaattc tggggagata tcatcaccaa caagctgaac gaggcgctcg
    10021 cagcacaagg cgataatgtg gtgattaatc tggcgtcaga tgagtatttt aaatcggtga
    10081 agccgaagaa attgaatgcc gagattatca agccggtgtt cctcgatgag aagaacggca
    10141 aatttaagat aatcagcttc tacgctaaga aagcacgcgg tctgatgagt cgtttcatta
    10201 ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt taatagcgaa ggttacttct
    10261 ttgatgaagc ttcctccagc aatggcgaac tggtgtttaa acgctacgag cagcgttaag
    10321 tttgcagtca atgccggatg cggcgtgaac gccttatccg gcctacaaaa gcatgcagat
    10381 tcaatatatt gcagaattca cgtaggcctg ataagacgcg ccagcgtcgc atcaggcgct
    10441 gaatgccgga ttgttacccg gcatttgtca tttagcggtg atgtttgcct ggaccatgac
    10501 cgccgtgatg atcatgagga gctttcttat ggtggcgcgg cggtggcggc ggtccgtgtg
    10561 ggtgccagcg attgcctcgc cattcataat gttgtttcca ccagccgtgg tcgcgccagt
    10621 gacctccatc ccagtaatag ccacgattat cacgatcgcc tatctgtaat tttactgacg
    10681 ggactaacgt aatttccgca gcctgtgctg ccatgggagc gaccagaacc agggaaagtg
    10741 cgagtacgat agattgcatc tttttcacag gttactcctt ttatcattgc ccgttatggg
    10801 tcgatatccg gagattacgc gatgagaacg acggttgtta ttctccgcaa tcctaatctc
    10861 taagttaccg catttattga gaatttctcc tttttccctg tcgtttttct ccataatttc
    10921 ttcataaaaa agccgggctg cataaaagca aacccggcct gattgagata atgaatagat
    10981 tttactgatg attcatcatc aatttacgca acgcagcaaa atcggcgggc agattatgtg
    11041 aaagcaaggg taaatcagca cgttctgcca gctcttttgg cagatccaac gtttcaccga
    11101 gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat
    11161 attcgcctgg attcaactgg tcacacagcg cacgatacgc tacggcagcg tgcggctccg
    11221 aggtgtagcc cagttctttt aactcacgca ttgtctgttg cgtggtttca tcatccacgg
    11281 ctgcataacc cagctctttc agttgccaga ttttgcggcg gaacaactct tccacacgcg
    11341 gccagttgtt cggctgactc acgtccatcg cgttggataa cgtcgcctga gtcgctttgg
    11401 gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc gttcacgttg gtcgcagcaa
    11461 taaaacgttt caccggcaga ccgagtgact tcgccagcag acccgccgtc aaatcgccga
    11521 agtttccgct tggcaccgag acaaccagct ggttgcgcgc ttcctgcggc agctgcgcaa
    11581 ctgcttcaaa gtagtagcaa atctgcgcca gcaaacggct gatgttaatc gagttagccg
    11641 agtttaaccc cagcgccact ttcagttctt catcatcaaa cgcctgcttc accagcgcct
    11701 gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat attgccgccc aacgtacaga
    11761 acagtttttc ttgcagtgga ctgattttgc ctcgtggata gaggataacc actttcacat
    11821 tcggcaaacc gtagaaagca tgagccactg ccgctccggt atcaccggag gtcgcggtca
    11881 gaatggtcac tggcttatcg cccgcaatat gggtcagcat ttgtgccata aagcgaccgc
    11941 cgaaatcttt aaatgccagc gttggcccgt ggaacaattc cagacaaccg acatcgcttt
    12001 caacattggc gaccggagcc gggaaggcaa acgccgcgcg cacgcgctct tccaggattt
    12061 cctgcgggat ttcatcacca ataaacgccg agaggatctt cgcactgcgg gtgacaaaat
    12121 ccagcttcag catctcatca atttcagtca ggctgaattc cggcaggtca tgcggaaaaa
    12181 acagcccctg atttttgccc aacccctggg ttacggcttg cgcaaagctg acctgctcgt
    12241 tgtgatcttt cagattgtag agtttcattt agttttccag tactcgtgcg cccgccgtat
    12301 ccagccggca aatatgaaca aaaccttcct gattttgcag gtagttctta cccaaccagt
    12361 cggcaacgcg ctgggcggta tccggcttgt cacatagagc gaacaaggtc gggccggagc
    12421 cggagatacc gctcgctacc gcgccgattt ccgcgaccgc ctgccgcgcc tgccggaagc
    12481 caggcagtaa ccgttcacgg tagggttcag cgataacatc tttcatcagc ttcgcggcaa
    12541 gctcaggctg acgggaatag caggcgtgaa tgaagccagc cagatgtcgc ccgtgcgcaa
    12601 tgcaatcctg gcggcgatac tgcgccggta aaatagcccg ggcttctgcc gtcgagactt
    12661 taattcccgg atacgccagc acccacagcc actcatcaaa ccctggcact tgctggctga
    12721 tgatgtcgtt ttcttcgatc atcaactgca taccaccaag aaaacacggt gccacgttgt
    12781 cgtaatgaat gctgccggag atacgtcctt ccagctcgcc catcaaagcc agcaaacggg
    12841 tgtcattaag tggcttgccg cagtgttcat tcatcgccat cagcgccgcg accaccgaac
    12901 aggcgctgga gcctaagccc gagccgatcg gcatattctt ttccagagtc atcgccactg
    12961 gaatttgctt gcccagctcc aggcaaaaac gctcccagca ctgataaacg atattttccc
    13021 gtggttctga cggcagctta tcggcaaagc gtccgaggtt gttgagactg aatgtctctg
    13081 ccgcctcaac cgtgactaca tctccgagca atgcaccatc aacgggtgtc accgccgccc
    13141 cgagcacatc aaacccgacg ctcatattgg cactggaagc cggggcataa actttaacca
    13201 tgtcagactc ctaacttcca tgagagggta cgtagcagat cggcaaagac accagcagcg
    13261 gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg gcagcggctg ataatagtgg
    13321 ctataaaagg ccagggcgtt ttcgccattt ttcactttga acagcggatc attcccatcc
    13381 acttcggcaa tcttcacgcg gcagacacca tcttcatcaa tattgccaac atagcgcaaa
    13441 acttttcctt catcacgggc cttcgccacg cgcgcggcaa agagatcgtc gagctgtgac
    13501 agattcgcca taaaagcggc aacatcaccc tcggcgttaa actctgcggg cagcacaggt
    13561 tcaatttcaa tatccgccag ctccagttca cgtcccgttt cacgagcgag aatcaatagt
    13621 ttacgcgcca catccatacc agaaagatca tctcgcgggt ccggttcggt ataacccatt
    13681 tcccgcgcca gcgtggtcgc ctcggagaaa ctcatgcctt cgtctaactt gccgaagata
    13741 taagaaagcg aacctgaaag aatgccggag aacttcacca attcatcacc tgcattgagc
    13801 agattttgca ggttctcaat aaccggtaat ccagccccaa cgttggtgtc atagaggaat
    13861 ttacgccgcg atttttccgc cgcataacgc aactgatgat agtaatccat cgacgaggtg
    13921 ttggcctttt tgttcggcgt gacaacgtgg aaaccttcgc gcaggaagtc ggcatattga
    13981 tccgccactg cctggctgga ggtgcagtca acaatgaccg ggttcagcag atgatattct
    14041 ttcacgaggc gaattaagcg cccgagatta aacggctctt tggcttgtgc aagttcttcc
    14101 tgccagtttt ccagattaag gccatgcaca ctagtgagca gagccttcga gttggcaacg
    14161 ccgcagacac gtaagtcgat atgtttattc ttcagccagc tttgctgacg cttcagttgc
    14221 tccagcagcg caccgccaac gccaccgacg ccaatcacaa acacttcgat aacctgatcg
    14281 gtattgaaca gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc gttatttacc
    14341 acgacagaga ttgagcgttc agaagatccc tgagcaatgg cgacaatgtt gatattggcg
    14401 cgggccagcg cggcaaagaa tttcgccgag atcccacgca aggtgcgcat accatcacct
    14461 accaccgaga taatggccag ccgttccatc accgccagcg gctccagtaa gccttctttc
    14521 agttccaggt agaactcttc ctgcattgcc cgttcagccc gcacacagtc gctttgcgga
    14581 acgcagaaac tgatactgta ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg
    14641 gcgcgtgaca tcgcggcaaa gacgcgcgcc gccatgccga ccattccttt catccccggg
    14701 ccggaaacgc tgaacattgc catgttattc agattggaaa tgcccttgac cggtaattcg
    14761 ccttcatcac gactggcacc aatgagcgta cctggtgctt gaggatttcc ggtattttta
    14821 atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg tgcgggggtg aagaacttta
    14881 gcgccgaagt aggaaagctc catcgcttcc tggtaggaca tcgacttcaa caacctcgca
    14941 tcgggcacct gacgcgggtc gcaggtatag accccgtcaa cgtccgtcca aatctcgcaa
    15001 caatcggcgc gtaaacaggc agccagcacc gccgcggagt agtcggaacc gttgcgtcca
    15061 agtaccacca gttcgccttt ttcattaccg gcggtgaagc ctgccatcag caccatgtga
    15121 tcagccggaa tacgacttgc cgcaatacgg cgggtggact cagcaatatc gacggtagat
    15181 tcgaggtaat gccccactgc cagtagtttt tcgaccggat cgataacggt aacgttgtga
    15241 ccacgcgctt ctaacacgcc ggccataatg gcgatcgaca ttttctcgcc acggcaaatc
    15301 agcgcagcgt taatgctatc cgggcactgc cctaacaaac taatgccatg caggacatgt
    15361 tttatttggg caaattcctg gtcgacgaaa gttttcaatt gcgccagcgg gaatcccggc
    15421 tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac gttcggcatc gctgatattg
    15481 ggtaaagcat cctggccgct aatggttttt tcaatcatcg ccaccaggtg gttggtgatt
    15541 ttggcggggg cagagaggac ggtggccacc tgcccctgcc tggcattgct ttccagaata
    15601 tcggcaaccc gcagaaaacg ttctgcattt gccactgatg taccgccgaa cttcaacact
    15661 cgcatggttg ttacctcgtt acctttggtc gaaaaaaaaa gcccgcactg tcaggtgcgg
    15721 gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt aatggtgatg
    15781 gtggtggtaa tggtggtgct aatgcgtttc atggatgttg tgtactctgt aatttttatc
    15841 tgtctgtgcg ctatgcctat attggttaaa gtatttagtg acctaagtca ataaaatttt
    15901 aatttactca tggcaggtaa ccagttcaga agctgctatc agacactctt tttttaatcc
    15961 acacagagac atattgcccg ttgcagtcag aatgaaaagc tgaaaaatac ttactaaggc
    16021 gttttttatt tggtgatatt tttttcaata tcatgcagca aacggtgcaa cattgccgtg
    16081 tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt cgaccagttt tatgtcatct
    16141 gccactgcca gagtcgtcag caatgccatg actcgttcgc gtaaagcttg cagttgatgt
    16201 tggtctgccg ttgtgtcact tttcgccggt tgttgtatta atgttgctaa ttgatagcaa
    16261 tagaccatca ccgcctgccc cagattgagc gaaggataat ccgccaccat cggcacacca
    16321 gtaagaacgt cagccaacgc taactcttcg ttagtcagcc cggaatcttc gcgaccaaac
    16381 accagcgcgg catggctcat ccatgaagat ttttcctcta acagcggcac cagttctact
    16441 ggcgtggcgt agtaatgata tttcgcccga ctgcgcgcag tggtggcgac agtgaaatcg
    16501 acatcgtgta acgattcagc cagtgttggg aaaactttaa tattatcaat aacatcacca
    16561 gatccatgtg cgacccagcg ggtggctggc tccaggtgtg cctgactatc gacaatccgc
    16621 agttcgctaa aacccatcgt tttcattgcc cgcgccgctg ccccaatatt ttctgctctg
    16681 gcgggtgcga ccaggataat cgttatacgc atattgccac tcttcttgac caaataaccg
    16741 cgaacagggt gatcactgtc aacttattac gcggtgcgaa tttacaaatt cttaacgtaa
    16801 gtcgcagaaa aagcccttta cttagcttaa aaaaggctaa actatttcct gactgtacta
    16861 acggtttaat tgttaaaaaa tgctacatat ccttctgttt acttaggata attttataaa
    16921 aaataaatct cgacaattgg attcaccacg tttattagtt gtatgatgca actagttgga
    16981 ttattaaaat aatgtgacga aagctagcat ttagatacga tgatttcatc aaactgttaa
    17041 cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg tgtccggccc
    17101 ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt tgacactgtc
    17161 gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt gatggaaagt
    17221 gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta atttgcagca
    17281 tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg caatttaggt
    17341 agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa cacgcaacac
    17401 gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag atggcgcgga
    17461 aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata tcaatctgcc
    17521 gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg ttgcgttgat
    17581 gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa tcggtgcaga
    17641 tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac gcaacctgct
    17701 gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg aaagctacaa
    17761 gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg atggcgagca
    17821 gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa acccaggcaa
    17881 aattcagtcc cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga aaccacacga
    17941 ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta cgccggatac
    18001 gccggaaatc atcgccacca tccacggtga aggttatcgc ttctgtggtg atctggaaga
    18061 ttaatcggct ttaccaccgt caaaaaaaac ggcgcttttt agcgccgttt ttatttttca
    18121 accttatttc cagatacgta attcatcgtc cgttgtaact tctttactgg ctttcatttt
    18181 cggcagtgaa aacgcatacc agtcgatatt acgagtcaca aacatcatgc cggccagcgc
    18241 caccaccagc acactggttc ccaacaacag cgcgctatcg acagagttga gcagtcccca
    18301 catcactcca tccagcaaca acagcgcgag ggtaaacaac atgctattgc gccagccttt
    18361 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
    18421 tgccacggta aaaccgatat gttcagaaag cgccagcaag agcaaataaa acatcaccaa
    18481 tgaaagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga gcgtttcaaa
    18541 aacaaagaac gccataaaag tcagtgcaat cagcagaatg gcgtacttag tcgcccggtc
    18601 cgttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcga ggaagttttc
    18661 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa accagctgct
    18721 ttgccattgc gcctgaaaac ctgactcgct aacttcccgt ttggctggca aaaaatcacc
    18781 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg
    18841 caccacagaa agatcgccgg taccgcttaa attcagggcc atattcagct tcaggttctg
    18901 cttccgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
    18961 ggtgccgggt tcaatggtca gcgccgttcc gttaacttca ggcgctttca ccacaccaat
    19021 accacgcgca tccccgacgc taatcacaat aaatggcttg ccaaaggtga tatttggcgc
    19081 gtcgagttcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
    19141 gacctgaccg gtataaatcc ctatcttgcg ttcttccacg ttctgattgc catcaaccat
    19201 caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcctc
    19261 ttcctgtacc gtataaagct cggtcacagg aatagcgata agcggcccaa caagtttttg
    19321 cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat cggcacgttc
    19381 gacaatcacc tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacag
    19441 ggtggtcatt ttccagaaca gggaggattt caacatagtc ttctcctttg caatggcagc
    19501 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
    19561 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
    19621 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
    19681 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt
    19741 atccagcacc ttaagcgtga cgtgttcctg atccacttcg gcgcttagcg ttatacgacc
    19801 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gatccagtaa
    19861 cgccggttca gcagcaacat taacctcggt gggcgtaaca tgtagagtga tgtttttttc
    19921 tgccaactgc acggtgcgcg cttcgctgac gcggcggaat aatgccgcca catcaacagc
    19981 agtcaaaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttccaccag
    20041 tgcctgcatt cgcgcatttt gcgtcagaat gttgtcagta aaacgagcca ctacttcagg
    20101 cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
    20161 ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
    20221 catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat cgggaagagg
    20281 aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa tggagcggtt
    20341 gatccaccaa accatgcctg cgccaatcac cagtgcgatc cccaacaaaa tggcgctggc
    20401 ccataaaatt cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt tcggtttgcc
    20461 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
    20521 cgaactttcg ggatcggcag gattttgcaa cgtgctacgc gcaccatact gaccacgcaa
    20581 cgttagccag acgtcattcc agcgcgaata atcctgccca acggctttat ttgccgaatc
    20641 gaacaatact ttgccctgcg catcggtcat atagacatga tattcattgc gcactttgtt
    20701 aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag
    20761 ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
    20821 tgcggtatcg attaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
    20881 aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa
    20941 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
    21001 atcggagaaa gatcggggtt gatggcgcgc agcttggcac gcaacgtttt aatgtgggta
    21061 tcgacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat tagttgctgg
    21121 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
    21181 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
    21241 tgaccaatac ggatgacggg agacggcgac gagaacttct tcacccgacg cagtaaggtg
    21301 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
    21361 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
    21421 aacgccggat ggagcgccag taactggcgg cacaattcaa agccgctgat atcaggcaaa
    21481 ccaacatcga gaatcatgac gtcgggaacc tgctggcgag ctttatccag caccggcaag
    21541 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg
    21601 gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt
    21661 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
    21721 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata aaagcgcacg
    21781 acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct
    21841 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatagcc
    21901 gcatcggagg tatcttccgc cagacccaat ccccctttaa taccacctgt tttcgcccgg
    21961 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
    22021 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
    22081 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
    22141 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
    22201 acaaaatctc aatactttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa
    22261 cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt
    22321 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
    22381 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
    22441 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
    22501 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
    22561 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
    22621 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
    22681 gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa tttgtcaccc
    22741 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
    22801 ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc aacgcgccga
    22861 ccattccgcc agtgctctac ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg
    22921 aacaagaggt attctatacc accgcgttag cccaggatca ggcagatggc tatgtactga
    22981 cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat gaaggtctgg
    23041 gaaccggcgt gcaggagttt atcctgacgg tatacggaac gtgcatgcca atgctcaacc
    23101 tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagac gccaaagcgg
    23161 gagatcgccc aattaatctg cgctgtgaac tgctgattcc tatccgccgt taacgctgca
    23221 gctcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca acgacccagc
    23281 cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag ttacgcagac
    23341 gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga catcccagtg
    23401 caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg caagattcca
    23461 gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga atacgcccgt
    23521 caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc agagaatcga
    23581 tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca aagatgatgt
    23641 cacagccgca ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct aaatcgctac
    23701 tgataatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct
    23761 cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact
    23821 gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt
    23881 aacgttaatt ttctgagtaa tgctgattat gcaccaagtt atctgtgcga ccaccaatcc
    23941 cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca
    24001 tattgcatcc gttgccgtcg agagcggtgt accggaacag ccctttggca gtgaggaaac
    24061 gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag aggctgattt
    24121 ttgggtggcg attgaagctg gcatcgatgg tgacagcact ttcagttggg tggtgattga
    24181 aaacaccagc cagcgcggcg aggcgcgttc ggctactttg ccgttacctg cggtgattct
    24241 gcagaaagtg cgtgaaggtg aggcgcttgg tcccgtgatg tcgcgttata ccggtattga
    24301 tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac tcactcgcgc
    24361 cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcacaatg ccgtgtatca
    24421 ggcattgtag gacggataag gcgttcacgc cgcatccggc attcggtgca cgatgcctga
    24481 tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc aacacctctt
    24541 ccagccactg gcgcagctcc acgggcgcgg ctttcaggct gttagatcca cgcgtaatcg
    24601 tcgcgatgcc cgcgccgagt tcatttttta actcacgctg actcatttcg ccacgcaaca
    24661 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
    24721 ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
    24781 gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
    24841 taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
    24901 gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct
    24961 catcaacgtc ggtttatccc ccatgaaata acggtagtaa gcgtcataag ccagcacgtt
    25021 cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca cagcgtcgat
    25081 acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt tataagctgc
    25141 tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt aactggtgcc
    25201 aatgttgata ttcgtttccg gatccagcaa ttgcccaggg ctgctataac caggaataga
    25261 gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc cgctggcccc
    25321 taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg ccatcgcata
    25381 gctttgcggg atctctttcc cactggtgta acgtttgaaa agatcgttgt aagccagcgg
    25441 gaatcgctct tccagatgat cccaaagctt cccggcgatc gttgcctgaa cgctaagatc
    25501 ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt ttgacttgct
    25561 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
    25621 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
    25681 atcaatcttc agctcatact cttctccgat gcgttgtgct gcaaccatcg ggtagaaacc
    25741 acgctgttgc atgagttgat gcaaaatctc tttcgcttca gcttcacgtc cgcgttccag
    25801 caataaatcc acctgccagt aacgccattc atctttctct tttgcttcca tcggcagacg
    25861 cgccagccag gtattcaggc cgcgacgatc gccggtgcca agtgccattc gcacacggcg
    25921 ttcaatcaac gacgtggatt gcgagcgcat aatgacgtca tcgcgccatt tcgcctgctc
    25981 gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg
    26041 atcttcatta agctgctgca cctgggcaag cgacgggatc atcagccgtg cattttcagc
    26101 atcctgccgc gccacactgg caaacgccac cgctgccatt tgacgggtaa aatcggtcgc
    26161 tccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg aaatgattgc
    26221 cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccagt
    26281 gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat cttgtttacc
    26341 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
    26401 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
    26461 gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg ctaacaagcc
    26521 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
    26581 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
    26641 atcatcggtg atctggcggt attccaggta gggataaagc ggataatcct tcagtccagg
    26701 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
    26761 acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
    26821 gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa gcgcatcctc
    26881 taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
    26941 tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg atcgaccaac
    27001 tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat cacgataaac
    27061 atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt
    27121 gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa
    27181 attggtgtcc tgggtctgaa cggcgcgggt aagtctaccc tgctgcgcat tatggcgggc
    27241 attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat tggttacctg
    27301 ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtccattga agaagcggtt
    27361 tctgaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta cgccgatccg
    27421 gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat cattcaggct
    27481 cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct acgtctgccg
    27541 gactgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt agcgttgtgc
    27601 cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat
    27661 gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgagggcac cgtggtggcg
    27721 attacccacg accgttactt cctcgataac gttgcaggct ggatcctcga acttgaccgc
    27781 ggtgaaggta ttccgtggga aggcaactac tcctcctggc tggagcagaa agatcagcgc
    27841 ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag
    27901 tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct ggcacgcttt
    27961 gaagagctga acagcaccga atatcagaaa cgtaacgaaa ccaacgaact gtttattcca
    28021 cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctacggt
    28081 gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcaat cgtcgggatc
    28141 atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctctgg tcaggaacag
    28201 ccggacagcg gcaccatcac tttaggtgaa acggtgaaac tggcgtcggt tgatcagttc
    28261 cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatt
    28321 atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa
    28381 ggggttgatc agggtaaacg cgttggtgaa ctttccggtg gtgagcgcgg tcgtctgcat
    28441 ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaacgac
    28501 ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc gggctgtgcg
    28561 atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat cctggactac
    28621 caggatgaag gtaaagttga gttcttcgaa ggtaacttta ctgagtacga agagtacaag
    28681 aaacgcacgc tgggcgcaga cgcactggag ccgaagcgta tcaagtacaa gcgtattgcg
    28741 aagtaatgcg taaaatgccg gatgcggcgt gaacgcctta tccggcctac aaaccaagaa
    28801 atttcaatac gttgtgaatc tccaggcctg ataagacgcg gtaagcgtcg catcaggcat
    28861 tgagcaccga atgccggata cggcgtgaac gccttttccg gcctacaaca gccttagtat
    28921 ccgcaatttc gtagcgggtc acccgtgcag gttttttcaa accttgttta atctcgacag
    28981 cctcttccag agaggttttc aactcatcaa aaaacttatt ttatacctcg cctttgagcc
    29041 gtttcatcgc ggttatctct gctcccccat catctcccgc accagctcca cgcagcgcag
    29101 gaaacggctg tcataatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    29161 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    29221 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    29281 atcaatcaac gcctgtacga acggatgctc acgcccttcg tactttttgc agaacgcctg
    29341 ggtagtgaca aaatcagtgt caataaacgc cactttattg gcatatttca ctgcaaaatc
    29401 aatgtattgt gcgtggccca gcgcgatttt atcgtaatcg gaatactgca atgcgatctc
    29461 atcaccgccg aggtgtgaaa agacataatc gcgaccatat tcccacgcac tggtggtatt
    29521 gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc caccaaggat
    29581 cgccacggta cgtacaaaga acggcttcac ttccgtagga atatattccc agtagcggaa
    29641 cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag
    29701 caccgtatcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    29761 gatcaggtcc ggctgaatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac
    29821 atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt
    29881 tttctgatat ttaaaagttt gtaataacca gcgcagacga tccggcaccg tgggctgctg
    29941 cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat
    30001 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    30061 cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgtcggggaa attcaagccc
    30121 caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag
    30181 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    30241 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    30301 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    30361 tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa
    30421 ccgtgtttcg ccgcttctga aattcgttcc tgaccgctgg gcaccgggcg gatctcccct
    30481 gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg
    30541 gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt cggttacctt cacgccgccg
    30601 accacgttca caaatacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca
    30661 gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc
    30721 atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac
    30781 accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa
    30841 gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc
    30901 gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc atcaccatcc
    30961 agaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag cgagccatct
    31021 ttggttacgt gcccaaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgcgtcaga
    31081 taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata
    31141 tgcatcacct ggatcgagtc aattaccatc agtttcggtt gctcttcttc ggcaatcagg
    31201 cagatctgtt cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagg
    31261 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cagtgacata cagcgttttc
    31321 atctgctggg ccagtttgca cagcgtttgc agtagcagcg tggatttccc cgcacctggg
    31381 ttaccgccaa tcagaatggc gcttcccggc accacgccgc cgcctagtac gcggtcgaac
    31441 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg
    31501 acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt
    31561 ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    31621 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    31681 tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc gagcaattac
    31741 ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    31801 gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct
    31861 gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg cgccagcggg
    31921 atttcatact cctgcgcgag gcgagtcaaa gttttcgctt tgtactgcgc gtctacgatg
    31981 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    32041 gcggtcaggc gcagcttgtc gcgcagatat tcagcaaaga aagtaaagcc gccggaggca
    32101 atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa gcctggcatc
    32161 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    32221 acacggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact
    32281 tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg
    32341 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc
    32401 cccagcgggg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg tgcggtgagt
    32461 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc
    32521 gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac
    32581 agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    32641 gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta
    32701 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    32761 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    32821 caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt gccttgttag
    32881 tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag cgtaatccac
    32941 agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt gcaccgctga
    33001 tgcgtaccga ctcaccagat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    33061 aaagccgcat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcccgttcag
    33121 gcgaaagcgt cgaagtgcgc gaccggctgg cgctcgacgg taaaaaagca ggcggctatt
    33181 ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc tatctgcgcc
    33241 tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    33301 ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc cgcacgctgc
    33361 tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    33421 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    33481 ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg
    33541 tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt ctctggcgca
    33601 atgccgacac ggtagtgatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc
    33661 ggatggaaga agataacgtc cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc
    33721 acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac gataaaacca
    33781 tctctacgtc gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc gaagcgtccg
    33841 gacgtaacga tctggtggtg aaaacagtcg aaggcgaccg caaagtctca ggctcggcct
    33901 atcgcgaaac caaagatcgt ggcttccacc acggcacttt gctgctcaat gccgacctta
    33961 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattacct
    34021 cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcacc catgagcagg
    34081 tttgcgaggc cataaccaag gcctttttcg cccattatgg cgagcgtgta gaagcggaaa
    34141 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgtcaga
    34201 gtagctggga atggaacttc ggtcaggctc cggcattctc gcatctgctg gatgaacgct
    34261 ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    34321 aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg cgactgcaag
    34381 gctgcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg gttgacttcc
    34441 cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg gtaaggtaac
    34501 tcgctgatta aaataccaaa ggaagggaat atgatcgatc tggaaaacca ggagcgggag
    34561 attatcaatt taatgttaag ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat
    34621 aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaatag
    34681 atcgaaaaga cggaacggct tagctcaaac atcaaaagca aaatgggaga aatttatggt
    34741 tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat
    34801 tcgtgaatag atacttgaat gtattttatt ttttatccat atgaaaaata atagtaaatc
    34861 tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtcc cccactccgg
    34921 cttaacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc agcagaacgg
    34981 attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg gcagtggtct
    35041 gctcgtgagt gcggatgtgg tcagacacag tgcagatggt cagggctttc gcgccaaatt
    35101 ctgcagcgac gccgtagata ccagccgctt ccatttccac gccgaggatg ccgtattttt
    35161 ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag aacaggttac
    35221 caacgcgagc atcaatgccc agtgctttag ccgcgtctac cgcgttacgc accatgtcaa
    35281 agtcagcgat agcggcaaag tcatgatctt taaaacggat gcggttaact ttggaatcgg
    35341 tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga actgcgccac
    35401 aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga
    35461 tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg cctttgtaag
    35521 taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag
    35581 tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg tcagcgaaat
    35641 cgcccatttc tgcattaatg tgtggggtag ccattgtttt atccttatca acatgttttc
    35701 accctctccc tgagggagag ggccggggtg agggcatcaa gccgcaccca ttatcagaac
    35761 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata
    35821 tccgcgaagg tttcacggtg acccagtgag cccggtttta ctttcgggcc gtaaaccagt
    35881 accggaatgt gttcacgcgt gtggtcagta ccggtccagg tcggatcaca accgtggtca
    35941 gcagtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg cagacggcgg
    36001 tcgaacagct ccagacccac agcataaccg gcgacgtcgc gacggtggcc ccaggaagag
    36061 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    36121 atggtggcgt caaacagcgc gtccaggcca gttgctttca ctttcttggt gataccgcag
    36181 ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct gaccgtgttt ttcatcaacc
    36241 agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta
    36301 cgctggaagt taccggcttt gtcaccgata aacggacgag cgataacacg accgatgttg
    36361 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccagg
    36421 ccaaaggttt cttcgtggca ggcaatctgg aacacagagt cagcggaggt atagaaaatc
    36481 ggcttgccgg ttttcatgtg ctcttcgccc agctgatcca gaatgaccgt accggaagag
    36541 tggcagttac cgaggtaacc cggcagatta gcgcgttcaa ccagtttatc cagcagctct
    36601 tgcgggaagc tgttttcgtg atcggagaaa tatccccact caaatagaac cgggacaccg
    36661 gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg
    36721 tacgcgccga taacttcagc gttgccgtcc attcccgccg gaatgaaacc ggtagaacct
    36781 tcgtgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg acctttacga
    36841 ccgttatcag cttcgccttt ggcacaagct tctgcgatat gacccagggt gtcagccccg
    36901 acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    36961 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca
    37021 gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg tgctttatcg
    37081 gcaagtttaa ttgccgcttt caccgctttc gccgcttcct gccagctgtt ttcgtctttc
    37141 gcgtggataa ccgccagcgg acgctgaccg tctacctggt cgcccaaacg cgccatatca
    37201 gtaaagccga cgctgtaatc aatggtgtca gatgcctgac gacgaccgcc gcccattgca
    37261 accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca
    37321 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    37381 gttgggcctt tttgtgccgc taccatacga ccaaagactt ctgctgcttt accgttgtcc
    37441 agcactgctt gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagata
    37501 agcatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg atactcaccc
    37561 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    37621 tgattcatgt cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    37681 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    37741 ttcacgtcca tcaccagcgc gtccagacct tccgcaagtt tcttcgccag aatagaggcg
    37801 gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta
    37861 tccgccggag ccagtgagct ggtctgaccg ataatcgcca cgccgacgtc tttaataatt
    37921 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    37981 agcgtaccgc cagtatgacc gaggccgcga ccagagatca tcggaatata gccgccgcag
    38041 gctgcgacca tcggccccaa catcagcgaa gtcacatcgc cgacgccacc agtggaatgt
    38101 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    38161 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    38221 atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatgcc gttgataaag
    38281 aaacgaattt cttcatcgct cagtgcatga ccatcacgtt ttttacgaat aatttcttgt
    38341 gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc
    38401 tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatca
    38461 ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcaccgtg gcccagcgct
    38521 ttcaacaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atctgcccag
    38581 tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc
    38641 acgccgcccg ctggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    38701 atcatgatgc gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg
    38761 atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    38821 ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    38881 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac
    38941 gggaacacaa cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc
    39001 gcgatttcga tgtcatcgtt accgtgtggg aagttggtta ccgtagcgat tcggatttcc
    39061 ggggtgccct gctctttcag cgttttgcga gcaatcggga taaagcgagg atagatacag
    39121 atagcggcgg tattgccgac cggggttttg gcctgatgac acagagcaat tactttctcg
    39181 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    39241 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    39301 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    39361 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    39421 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    39481 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    39541 gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca
    39601 ttttctggca ctggaagcag aaaacaatct gccttatccg cagcttcctg ccgaagcccg
    39661 ccgcgcgctg gatgagggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    39721 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct
    39781 ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt
    39841 accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccata
    39901 tgttagaatt ctaacacaag acgagatcga tgttcgaata aaacgtttct ggcgttacct
    39961 cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    40021 tacccgcgcg atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt
    40081 tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg
    40141 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    40201 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    40261 ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac
    40321 gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt
    40381 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    40441 ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct
    40501 gtgtaagaaa gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg
    40561 ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg
    40621 gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc
    40681 gggcgcgcgt ttgccgtatg gtgatgagcc agatccgatc acccatctgc aaactgtcgc
    40741 cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat
    40801 caaacgtaat ccgcaggcgc tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    40861 tgaatttact gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg
    40921 tttgtcggat ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    40981 cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    41041 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca
    41101 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    41161 ggacgatggg acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt
    41221 tgcagattgt tgacggcaaa gtggtgtgga acgctgtggt ttgcgagcag tgtgatacct
    41281 gcctgaagag gtgtccgcaa catgccacgc caatggcgca atccatgagc gtggacgaag
    41341 tgcttagcca tgtccgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg
    41401 aagccacgac ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc
    41461 aactgcgcca cctcacctgc ctggtggaca gtaacggcat gttgagcgaa accggctggg
    41521 aaaaattact cccggtgtgt gacggcgcaa tgctcgatct caaagcgtgg gggagcgaat
    41581 gtcatcaaca cttaaccgga cgcgataatc agcagattaa gcgcagcatc tgtttgcttg
    41641 cagagcgcgg caagctggcg gaactgcgtt tgctggtaat tccagaccag gtggattatt
    41701 tgcatcacat cgatgagctg gcgacgttta tcaagagact tggcgatgtt ccggttcgcc
    41761 tgaatgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc
    41821 cggaagacgt tgagccgttg gctgatgcgt taaaggtgcg cggggtgagc cggttgatat
    41881 ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg
    41941 catgataaga cgcgacaagc gtcgcatcag gcattatgcg ccgactgccg gatgcggcgt
    42001 gaacgcctta tccggcctac ggttcggcac agacttgtag gcatgataag acgcgtcagc
    42061 gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt gaacgcctta tccggcctac
    42121 ggttcggcac agacttgtag gcatgataag acgcgtcaag cgtcgcatca ggcattgtgc
    42181 gccaactgcc ggatgcggcg tgaacgcctt atccggccta cggttcggca caaacctgta
    42241 ggcatgataa gacgcgttaa gcgtcgcatc aggcattatg cgccgactgc cggatgctgc
    42301 ctacgaaggg taggctaccc actaacgcta aacaacgcat acgtattatt aagcaacact
    42361 tcggcaatct catcttctgg ttccgggcgc aactcgcaaa gcacgtcaaa cacccgcaca
    42421 gcctgttccg gtcggttagg ctgcccctga aagccgttga gcggcatatc cggcgcgtcg
    42481 gtttccagca acaacgatgc cagcggtaat tttgcgatga catcgcgggt tttactggcg
    42541 cgtggatagg tgatagtacc gcctacgccg attttgtagc ccagctgtac aaaccgctcg
    42601 gcctgttgca ggctgccgga aaaaccgtga accacgccag tgcacggtaa atcgtgacgt
    42661 ttaagatgca tcgccagttt gtcgtgtata cgccgggaat gaaggatcac cggcagatcg
    42721 tagcgtttcg ccagtttcag ctgttcgtcg agtaaccact gctgcctctc aaattgcgga
    42781 tcgtcgccga agagatccag accgatctcc cccaccgcca ccaccttcgc cggacgcctt
    42841 tccagcgcct gctgtagctg atcaagagac acatcgctat gtttttccaa catccccgga
    42901 tgcaacccca atgcggcata cagcggttga tatttttccg ctaacgcctg cacgcgggca
    42961 aaattttccg cctctgtcgc cggaacaatg atcttgccta cgcccgcttg tgccgcgcgt
    43021 tgcaggctgg cctcttcatc gccactaaac ggcgggaaat caaaatggca gtgggtatcg
    43081 ataaaacggc aaatcaagcc agatcctcat tgttaaatgt cgggtcgttc gcctgcggag
    43141 catcgctcac ttcggcaacc agcgtatcgt tggcgactgg cgcaggcgga ataacgatcg
    43201 attccggcgt caccactggc accagatggc gggtaagcgg cgctttttca gttaatagct
    43261 tgccaaccgt ggcgaggaaa taacgaccgc ataaacgccc aagtttatag tcttcacgca
    43321 gcgccggaat ccgactgcca agcgcgatac tatgtaatgg cttcggcgga taaatttcga
    43381 atatccgcag cttgcccggt ggtttctcaa taaattgctg aatgtcacga tagctggttt
    43441 catgatgctg caccagattg accagcggct gcaggctact gtcacccagc cagcgttcca
    43501 tgcgtttgaa ccactgcggc gtgtagtaca tttgtgacgg cacagtgcga atgacgacca
    43561 acgttttagc gccctgcctt gccgcctctt taaccggaat cgcatcactg atcccgccat
    43621 ccaggtagtt aatgccttcc agcgacactc cgctacgata aaagccaggt atcgcactgg
    43681 aggcgcgaat cacatccagc cagttttgtt tggttggtaa aaagtaatta ggcgcgtagt
    43741 catcctgacg acaggcgcac atataaaacg atttgccgct gtcaaacaac cgcgcggcgg
    43801 tatccatttg caacggcatc tggcttgcgg tggcctccac cagccagtcg agatcgataa
    43861 gatttcctcc acggacaaag cgcaatggat cgaaaaattc gcgttttgtg gtatagcgca
    43921 tgatgacttt gcgcgcgtaa ccgggctgat tgcagataaa cgccgagagg ttctgcgccc
    43981 cggcagatgt gccgagataa agatcgaaag gattaaactg cgcgcgcata aactcatcca
    44041 gcacgccagc cgtgaaaatt ccacgctgtc cgccgccttc gcacaccaga gctattcgtc
    44101 caggctggaa cggccttagc gacaacggcg caatattacc aagcgttaca ggtattcgct
    44161 gccccacctc tgccttcctg tttttgttgt gaaagaatga caaagtaacg caatttatta
    44221 ttcgctaaaa ccgcaaaagc cagtccgctg gactggcttt ggataaaatt ttcttaatgg
    44281 tctgaaatct agggtcgttt tcggcccatg aacaaactca ccaggaacag aataatcccg
    44341 acgacaaaga caattttagc tgcgcctgca gcggtaccgg ccagaccacc aaacccaagt
    44401 gcggcggcga ttaacgcgat aaccagaaat atgatgcccc aacgaaacat aagcgtctcc
    44461 tttaccatag ttaatgtcac cgcttgatat gagcgtgaaa atcactcaat gcgatatttt
    44521 tagtgtggtg cacatcgcgc ctcccgacaa agttcgggag gacgaattac gacgaattac
    44581 ttagttttca gatcattttt aacgcttttc acaccatcta ccgctttggc gatactttca
    44641 gcacggtcgc tctgtgcctg ggaatcgacg gtaccggaga gctgaaccac gccgtcggtg
    44701 gtttcaactt tcacatgacg ggaagggacg atatcgtccg ccagcagttt ggctttgatt
    44761 tcactggtgg tggcggtgtc acccgcgtag cccttcacag agccttcttt agcgtcgcga
    44821 acgtgcagtt tgtcgctgac agaggtcacc ccttcaacgc ctttcgccac tttcactgcc
    44881 tcttcggcct gagcctggct ttcaacgaaa ccgctcaggg tcacgacttt ttgatcggtt
    44941 ttgacagaga tatcggtgct cttgatgttg tcatgatcca ccagcgccgc cttcactttc
    45001 gcggtgatgg cgctgtcatc catgaaatta ccgactttat tcatagagct atcgactttt
    45061 tgccctgcgc tttcattggt agtctgtgcg ttgttttccg cgtaggcaga gccggtcgcg
    45121 acggcagagg tcaacattac agccagcaga gttttcgaaa tcttcagtct tgtcatagtc
    45181 atcgatttat tcctgtatgt ttgctcgtaa tttgagcctg gcaacacgag gttgcattgc
    45241 tgaataggga gagacttcac cctctacaga agtcaatggt cgccatcaca aaagcgatgg
    45301 gtgatgaata acgaccatta cagcctctga atcagttatt aatatcggta gaatgacaat
    45361 cgacggcttt agatactgat atctacgcat tgaacggtat ttaacgccgt cagaaatgtc
    45421 attactttgt taaatataga tcacaatttt gaaaccgctc gggatatcag cgaaaacata
    45481 agcaaaagtg aatgttttaa gaacattccg taagcggcta ataaggaagg gaaattgaca
    45541 gggcgcagcg gctaccgcgc cctggaggca agaattaatg ctcgcgggtc tggtggaact
    45601 gaacgtcggg ataacgttcc tgcgccaggc gcaggttgac catgctggta gcgatgtaag
    45661 cgaggttatc gccgccatca agcgccagtt ggctttcgtt cttacgcttg aactcttcga
    45721 atttcttcgc gtccgcacat tctacccagc gggcagtggc aacgttgact gattcatata
    45781 ctgcttcaac gctgtattcg ctcttcaggc gcgataccac cacatcaaac tgcagcacac
    45841 caacagcacc aacgatcaaa tcgttgttgg agatcggacg gaacacctgc accgcgccct
    45901 cttcggaaag ctgtaccagc cctttgagca gctgtttttg cttcagcgga tctttcaggc
    45961 ggatacgacg gaacagttct ggtgcgaagt tcggaatacc ggtgaacttc atcatctcac
    46021 cctgggtaaa ggtgtcgccg atctgaatag tgccgtggtt gtgcaggccg aggatatcgc
    46081 ccggatacgc ttcttcaacg tgcgaacggt cgcccgccat aaaggtcagc gcgtcggaga
    46141 taaccacatc tttcgcagta cgcacctggc gcagcttcat gcctttttca tatttaccgg
    46201 atactacgcg cataaacgcc acgcggtcgc ggtgtttcgg gtccatgttg gcctgaattt
    46261 taaatacgaa gccggtaaat ttgtcttcgc tcgcctctac ggtacgggta tcagtctgac
    46321 gcggcatcgg cgcaggagcc cactccacca ggccatccag catatgatca acgccgaagt
    46381 tacccagcgc agtaccgaag aataccggag tgatttcgcc cgcaaggaac aattctttgt
    46441 cgaactcgtt agacgcgcct ttcaccagtt ccagttcgtc acgtagctgc tgtgccagat
    46501 cttcaccaac cgcagcgtcg agatccgggt tattcagccc tttaacaata cggacttcct
    46561 gaatcgtgtg gcctttaccg ctctgataga gataggtttc gtctttataa aggtggtaaa
    46621 cgcctttaaa cagcttgccg cagccaatcg gccaggtgat cggtgcgcag ccgattttca
    46681 gctcgttctc aacttcatcg agcagttcca tcgggtcgcg gatatcacgg tcaagtttgt
    46741 tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac ttccatcagc ttacgggtac
    46801 gatcttcaac accttttgca gcgtcgataa ccatcaggca gcagtccacc gccgtcaggg
    46861 tacgataggt atcttccgag aagtcttcgt gccccggggt gtcgagcagg ttaaccaggc
    46921 aatcgtgata cggaaactgc atcacagacg tagtaatgga gatcccacgc tgcttttcca
    46981 tctccatcca gtccgactta gcgtgctggt tggaaccacg gccttttact gtaccggcgg
    47041 tctgaatggc ctgtccgaac agcagcacct tctcggtgat ggtagtctta ccggcatccg
    47101 ggtgagaaat aatggcaaaa gtgcggcgct tcgccacctc ttgcaaataa ggagacaacg
    47161 tcataattct ttcttcttga gtaagcggca tcgcgccgcg cttgttgaaa acgaaaaatt
    47221 gcggctattt tacccatcaa cgggggggag gcaatcagtg tttacacagg agctgctcca
    47281 gttcgtgcaa cgaagaaacg gtccaggtgg gcgcgatgcc ttctggttgc tcgcgattgt
    47341 gcgcattcag ccagcaggtc gcaagcccgg cgttgatgcc accgagaata tcggactcgg
    47401 cagtgtcgcc aaccatcagc acgcgtgaac ggtcaggatt gcccgcctgt tccagcgcat
    47461 aatcgaaaat tttcttattc ggtttggcaa cgccaacttc ttcggaaatc accagcaaat
    47521 cgaagtaatc acgcaggccc gtgcgttcca gacgcacttg ctgcaaggcg ctaaagccgt
    47581 tggtgatgat gccgattttg gcgttgccac gaatggcgtt aagcagagaa accgcgcccg
    47641 gcagcggcgt gcagatttcc gccatcgcat taataaaggc atcgttaagc aagcctgctt
    47701 caactttcag ccgttcggcc cagctctcaa aacgcccgtg ctgaagctgt aatgaagtga
    47761 tcgcgccgtt ttgataatcc acccacagtg gcttgttaac ggcctgatag tcctgaaaat
    47821 cttcagcggt aaaggtgacg ctgtaatcaa gaaacatccg ctgcaggccg gtgaatgagt
    47881 caaaggtaaa cagcgtttca tcggcatcaa agaaaatcca gtcccacttc attattccac
    47941 cttgtcttac atactgattg gcaacgccat gatgatggcg tcttcgcgac cgtccgtggt
    48001 ggggtagtaa ttgcggcgaa tcgtcgcctc gttaaagcct aaactttcgt acagggcaat
    48061 ggcggcagca tttgaggcac ggacttccag ccatagtgtc gccacgccgc gtttttccag
    48121 ttcgtcgatc agatgttcca gcagcaccct tcccaatccc tgacgctgat agtcaggatc
    48181 gaccgcaata ttgaacaatg tagcttcatc cagcaccact tgcgtaatcg caaacgccgc
    48241 cattttgccg ttttgcgtta actgaaagtt gagataacgc tcgccctggt tgctggcaaa
    48301 ggttttttca ctccacggaa aggcgtgggc gcgttgttca atgtggtaag ccgctggtaa
    48361 atcagtcgtt tcgagggaag aaatcgtgtt catatgtgca aatctgttgc cataacgcgg
    48421 cgcgtgccgt tgggtttgcc cgtaattcgg tgagcgccgg tgatgccacc tgagcgcctt
    48481 ccagtgatag cggttcgtca gtacccaacc gccagctgtt gcagcgactg ccttgcggca
    48541 gcatcgcgat tttttctggc gtcagttgca gcacttgatc ggggctgacg gttaatgcgc
    48601 gcagaacatc gctcactaaa ggatcggtca gggcgggaag atcgtttgcc accatcacca
    48661 gacggacgtg tgccgggatc gcaatggcaa tctccccctg caacgcgcca gggcgacgca
    48721 gcgaccactg ggtaatgccc agttgctgta actgccagtc tcgtcgggat gtcatagcga
    48781 atcgctcctg ttgtcagggg cgcaaatata gcaaattcgt cgataccgcg ccaacatatg
    48841 gctataatcg ccgccagtat caattgagga gcattccatg tctgcattta ccccggcaag
    48901 tgaagtcttg ctgcgtcaca gtgatgattt cgaacaaagc cgtattctgt ttgccggaga
    48961 cttacaggat gacctgcccg cgcgtttaga taccgcggcc agccgtgctc atacccagca
    49021 attccaccac tggcaggtat taagccgcca gatgggggat aacgcccgtt tcagtctggt
    49081 cgccacggtg gatgacgtcg cagattgcga tacgctgatt tactactggc cgaagaacaa
    49141 accggaagcc cagttccagt tgatgaattt actttctctg ctgccagtgg ggacagatat
    49201 ttttgtcgtt ggcgagaacc gcagcggcgt gcgcagcgcc gagcagatgc tggcagatta
    49261 tgcgccgttg aataaagtcg acagcgctcg tcgctgtggc ctctattttg gtcgtctgga
    49321 aaaacagccg gtatttgatg ccgataaatt ctggggcgaa tacagcgtcg atggcctgac
    49381 ggtcaaaacg ctgcctggcg tgtttagccg cgacggtctg gatgtcggta gccagttgct
    49441 gctctcgacg ttaactccgc acacgaaagg taaagtgctg gatgtcggct gtggcgcggg
    49501 ggtgctttca gttgcctttg cgcgccattc gccgaaaatt cgtctcacct tgtgcgatgt
    49561 ctctgcgcca gcggtagaag ccagccgcgc aacacttgcg gccaactgtg ttgaaggtga
    49621 agtctttgcc agcaacgtct tttccgaggt gaaaggtcgt tttgatatga tcatctccaa
    49681 cccgccgttc cacgatggga tgcaaaccag cctggatgcg gcgcaaacgc tgattcgcgg
    49741 cgcggtgcgt catcttaata gcggcggcga gctgcgaatt gtagcgaacg ccttcctgcc
    49801 ttacccggac gtgctggatg agacatttgg cttccatgaa gtgattgcgc aaaccgggcg
    49861 tttcaaggtg tatcgcgcca ttatgacccg ccaggcgaag aaaggttaat tatctcgccg
    49921 aataccgtgt cggatgcggc gtgaacgcct tatccgacct acaaaacctg cacgttagcc
    49981 cttcgtaggc cagataagac gcgccagcgt cgcatctggc atctgccccc gcatgcctac
    50041 caaacctgca cgctagtttc ctgatggaca tttttccagc aattacacct ccggcgataa
    50101 ttaactattg acgaaaagct gaaaaccact agaatgcgcc tccgtggtag caattctttt
    50161 taagaattga tggtatgcga aggtggcgga attggtagac gcgctagctt caggtgttag
    50221 tgtccttacg gacgtggggg ttcaagtccc ccccctcgca ccaaaaacca cgttgatatt
    50281 gctcgcactg ggcgaaggtg gcggaattgg tagacgcgct agcttcaggt gttagtgttc
    50341 ttacggacgt gggggttcaa gtctcccccc tcgcaccaaa cgaggcgata tcaaaaaaag
    50401 taagatgact gtgcgaaggt ggcggaattg gtagacgcgc tagcttcagg tgttagtgtc
    50461 cttacggacg tgggggttca agtccccccc ctcgcaccaa ttatctttac ttcctttctt
    50521 gtttcttcct tgatttttca tccctgattc aaattcatta tcatcagcat gatggcagca
    50581 ataagcgcca ggcacgatgg tagcaaaacg taatgccgta actgtttgtg atacagcatc
    50641 accggcgtca atactaatcc catcagaatg acaatccgcc actgatccgg cgataaatcc
    50701 gcatacatca gccctgcaat cagcaatccc ggcagcaaca ctccccagcc actgcccagc
    50761 gtacgttgca acatggtttc atctctttca ttgataatga taaccaatat catatgataa
    50821 tttttatcat ttgcaagcca gagaaatccc ttgctatcgg gtaaacctat cgctaggatt
    50881 agcaatcatt atcatttaga ttactatccc gattatggcc tatcgttccg caccgctcta
    50941 tgaagatatc atctggcgaa cgcatctcca gccgcaggat gctgggcttg cacaagccgt
    51001 acgtgcgacg atcgccaaac atcgtgaaca tttgctcgag tttatccgcc tggatgaacc
    51061 cgctccgctt aacgccatga cgctggcgca atggtcatca ccgaatgcgc taagttctct
    51121 actcgcggtc tattccgatc atatctatcg caaccaacca acgatgatcc gcgagaacaa
    51181 accgctgatc tcactatggg cacaattgta tatcggcctg atggtgccac cattaatgct
    51241 ggcgctactg acgcaggaaa aggcattaga tgtgtcgccg gaacatttcc atgctgagtt
    51301 tcacgaaacc ggacgcgtcg cctgtttctg ggtcgatgtg tgtgaagata aaaacgcaac
    51361 accacattcg ccgcagcagc gaatggaaac gttaatcagc caggcgctgg ttccggttgt
    51421 gcaggcacta gaagcgaccg gagagatcaa cggcaaactt atctggagta ataccggtta
    51481 tttgatcaac tggtatctca ctgagatgaa gcaactgctc ggcgaggcga cggttgaatc
    51541 gctgcgccat gccctctttt ttgagaaaac gctcacgaac ggtgaagata atccactctg
    51601 gcgtaccgtc gtgctgcgcg atggcctgct ggtgcgccga acctgctgcc agcgttatcg
    51661 cttaccggat gtgcagcaat gtggcgattg tacgctgaaa tgaaagcact gccggggaag
    51721 taaacccggc atcatgcgga ttaataggga tgcaacacat tacttgcttc gcaatgccgc
    51781 atacataaca gaatatctgc ggcctccaac aaccctgcat cagaactgac gcccagtttc
    51841 gacatcacat taaatttatg tgcacggatc gttttgatat tgcgtttaag ctgctcggca
    51901 atttgtgtca ttgagtagcc acgcgacata aagcgcaata tttctctctc cgtcgggctt
    51961 agcgtccggc tttggttaat gtaccactga ttgttcagtc ggtcggtcgc ctgacgtaca
    52021 ccatttaatg acaaaaagag ttcctgatga aaaatctcca gcgttgacgc tttacttaat
    52081 acaccgtcca gcggtgatgg cgacaatgaa ccaatcagcc gagcttcaat atcatcatcc
    52141 gcaataacta aacgccgggt acgcggaaac ttaatcgcca gttcagtcag gcaagataat
    52201 ccctctctgc gctcacttct catggcagaa aaagaaaaaa tgaccgccgc aaatgagatc
    52261 cgcgacatgg catcctgaaa tgactcctga ctggtatagg tatggagctt ataagggtta
    52321 cccgcaaact ttctgaataa actctcaata ccgatactgc tcatgacgca tttttctatg
    52381 agtgcgacat ttctcttctt aattcggctg tgttccattc tgcggcatct ccataaacag
    52441 aaggcaagtt aagttctctt gcaccctgcg cgctatttat ccatgcatac atttcagcgt
    52501 tgctgtgaat tgccagtcga cgcatcgcac tgtttttttg ggcgctgata gttttattac
    52561 ttttcttaag caacgaagct atctggttga tgccccatcc tttacctaat agccgcagta
    52621 ccttgcgctc tgaaagagtg agaatgacgg agcgatagct aaaatcatga gtttcattca
    52681 ttgccggaga ggtcagcatg gcgctgatac gctctgcgtg cgtattgccg gaacgtacgg
    52741 tcttgaccag attctcaatg ggttcaacat cagacaataa cgtcgccgta gggcacatga
    52801 gcaattctac tgcctgggaa taccaggagc gtgacaccat gaaaacccag tgaatttccc
    52861 gatattgtga gattaaagaa taataatgtt cacaaacaga acgtggatct tcactttcac
    52921 cggctaaatc agcaatgact aatccggaac gacgtaattg taataatgta aggtcctccg
    52981 cagaggcgct ggatattatt tcatattcag ggaagtgagt cctcatgacc t
//
LOCUS       DAQSWQ010000019        51284 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.019, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000019 DAQSWQ010000000
VERSION     DAQSWQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.019"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            236..1138
                     /locus_tag="R8G18_001148"
     CDS             236..1138
                     /locus_tag="R8G18_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9794911.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1285..2505
                     /locus_tag="R8G18_001149"
     CDS             1285..2505
                     /locus_tag="R8G18_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEE9794912.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2478..3107
                     /locus_tag="R8G18_001150"
     CDS             2478..3107
                     /locus_tag="R8G18_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEE9794913.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(3108..4268)
                     /gene="ybdL"
                     /locus_tag="R8G18_001151"
     CDS             complement(3108..4268)
                     /gene="ybdL"
                     /locus_tag="R8G18_001151"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HEE9794914.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4377..5465
                     /gene="hcxA"
                     /locus_tag="R8G18_001152"
     CDS             4377..5465
                     /gene="hcxA"
                     /locus_tag="R8G18_001152"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HEE9794915.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5475..5672)
                     /locus_tag="R8G18_001153"
     CDS             complement(5475..5672)
                     /locus_tag="R8G18_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEE9794916.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5854..7959)
                     /gene="cstA"
                     /locus_tag="R8G18_001154"
     CDS             complement(5854..7959)
                     /gene="cstA"
                     /locus_tag="R8G18_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HEE9794917.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(8140..8553)
                     /gene="entH"
                     /locus_tag="R8G18_001155"
     CDS             complement(8140..8553)
                     /gene="entH"
                     /locus_tag="R8G18_001155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HEE9794918.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8556..9302)
                     /gene="entA"
                     /locus_tag="R8G18_001156"
     CDS             complement(8556..9302)
                     /gene="entA"
                     /locus_tag="R8G18_001156"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HEE9794919.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9302..10159)
                     /gene="entB"
                     /locus_tag="R8G18_001157"
     CDS             complement(9302..10159)
                     /gene="entB"
                     /locus_tag="R8G18_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HEE9794920.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(10173..11783)
                     /gene="entE"
                     /locus_tag="R8G18_001158"
     CDS             complement(10173..11783)
                     /gene="entE"
                     /locus_tag="R8G18_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HEE9794921.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11793..12968)
                     /gene="entC"
                     /locus_tag="R8G18_001159"
     CDS             complement(11793..12968)
                     /gene="entC"
                     /locus_tag="R8G18_001159"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HEE9794922.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13343..14308
                     /gene="fepB"
                     /locus_tag="R8G18_001160"
     CDS             13343..14308
                     /gene="fepB"
                     /locus_tag="R8G18_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HEE9794923.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(14389..15639)
                     /gene="entS"
                     /locus_tag="R8G18_001161"
     CDS             complement(14389..15639)
                     /gene="entS"
                     /locus_tag="R8G18_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HEE9794924.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15750..16754
                     /gene="fepD"
                     /locus_tag="R8G18_001162"
     CDS             15750..16754
                     /gene="fepD"
                     /locus_tag="R8G18_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HEE9794925.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16751..17743
                     /gene="fepG"
                     /locus_tag="R8G18_001163"
     CDS             16751..17743
                     /gene="fepG"
                     /locus_tag="R8G18_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HEE9794926.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17740..18555
                     /gene="fepC"
                     /locus_tag="R8G18_001164"
     CDS             17740..18555
                     /gene="fepC"
                     /locus_tag="R8G18_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HEE9794927.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18552..19685)
                     /gene="fepE"
                     /locus_tag="R8G18_001165"
     CDS             complement(18552..19685)
                     /gene="fepE"
                     /locus_tag="R8G18_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HEE9794928.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19901..23782)
                     /gene="entF"
                     /locus_tag="R8G18_001166"
     CDS             complement(19901..23782)
                     /gene="entF"
                     /locus_tag="R8G18_001166"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HEE9794929.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(23779..23997)
                     /gene="ybdZ"
                     /locus_tag="R8G18_001167"
     CDS             complement(23779..23997)
                     /gene="ybdZ"
                     /locus_tag="R8G18_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HEE9794930.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(24000..25202)
                     /gene="fes"
                     /locus_tag="R8G18_001168"
     CDS             complement(24000..25202)
                     /gene="fes"
                     /locus_tag="R8G18_001168"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HEE9794931.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            25445..27685
                     /gene="fepA"
                     /locus_tag="R8G18_001169"
     CDS             25445..27685
                     /gene="fepA"
                     /locus_tag="R8G18_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HEE9794932.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27860..28480
                     /gene="entD"
                     /locus_tag="R8G18_001170"
     CDS             27860..28480
                     /gene="entD"
                     /locus_tag="R8G18_001170"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HEE9794933.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28602..28754)
                     /gene="hokE"
                     /locus_tag="R8G18_001171"
     CDS             complement(28602..28754)
                     /gene="hokE"
                     /locus_tag="R8G18_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HEE9794934.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(29105..29257)
                     /gene="hokE"
                     /locus_tag="R8G18_001172"
     CDS             complement(29105..29257)
                     /gene="hokE"
                     /locus_tag="R8G18_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HEE9794935.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            29697..30815
                     /locus_tag="R8G18_001173"
     CDS             29697..30815
                     /locus_tag="R8G18_001173"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEE9794936.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            30881..31129
                     /locus_tag="R8G18_001174"
                     /pseudo
     CDS             30881..31129
                     /locus_tag="R8G18_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            31194..31562
                     /locus_tag="R8G18_001175"
     CDS             31194..31562
                     /locus_tag="R8G18_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEE9794937.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            31656..32309
                     /gene="nfsB"
                     /locus_tag="R8G18_001176"
     CDS             31656..32309
                     /gene="nfsB"
                     /locus_tag="R8G18_001176"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HEE9794938.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            32417..33664
                     /gene="ybdG"
                     /locus_tag="R8G18_001177"
     CDS             32417..33664
                     /gene="ybdG"
                     /locus_tag="R8G18_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HEE9794939.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(33732..35108)
                     /gene="pheP"
                     /locus_tag="R8G18_001178"
     CDS             complement(33732..35108)
                     /gene="pheP"
                     /locus_tag="R8G18_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HEE9794940.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITVAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(35210..38353)
                     /gene="cusA"
                     /locus_tag="R8G18_001179"
     CDS             complement(35210..38353)
                     /gene="cusA"
                     /locus_tag="R8G18_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HEE9794941.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(38365..39588)
                     /gene="cusB"
                     /locus_tag="R8G18_001180"
     CDS             complement(38365..39588)
                     /gene="cusB"
                     /locus_tag="R8G18_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HEE9794942.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(39604..39936)
                     /gene="cusF"
                     /locus_tag="R8G18_001181"
     CDS             complement(39604..39936)
                     /gene="cusF"
                     /locus_tag="R8G18_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HEE9794943.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39960..41342)
                     /gene="cusC"
                     /locus_tag="R8G18_001182"
     CDS             complement(39960..41342)
                     /gene="cusC"
                     /locus_tag="R8G18_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HEE9794944.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            41499..42182
                     /gene="cusR"
                     /locus_tag="R8G18_001183"
     CDS             41499..42182
                     /gene="cusR"
                     /locus_tag="R8G18_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HEE9794945.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            42172..43620
                     /gene="cusS"
                     /locus_tag="R8G18_001184"
     CDS             42172..43620
                     /gene="cusS"
                     /locus_tag="R8G18_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HEE9794946.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            44357..46258
                     /locus_tag="R8G18_001185"
     CDS             44357..46258
                     /locus_tag="R8G18_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEE9794947.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            46286..46747
                     /locus_tag="R8G18_001186"
     CDS             46286..46747
                     /locus_tag="R8G18_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HEE9794948.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            46767..51178
                     /locus_tag="R8G18_001187"
                     /pseudo
     CDS             46767..51178
                     /locus_tag="R8G18_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT        12427 a        14013 c        13294 g        11550 t
ORIGIN      
        1 catcacccat tatgggaaat aaataaaagg taattcatgg atggttgaac tatatccctg
       61 cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg ctgtgctatt
      121 ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat acatacgcaa
      181 aaaacattga ttaagtgaat ataccatgga agaaaaatat aaaccggagt agtgtatggc
      241 caatctctac gacttgaaaa aattcgactt aaatcttctt gtcatatttg agtgtattta
      301 ccagcattta agcattagca aagctgctga atcactatat atcaccccat cagccgtgag
      361 ccagtcgtta caacgcttac gtgcgcaatt taatgaccct gtattcattc gctcaggaaa
      421 aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga aaaacctcaa
      481 aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa actttattat
      541 ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct gcctacggca
      601 agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga atgctgaaga
      661 actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga tcagccgttc
      721 cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta ataagcatcc
      781 gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca cccaattaat
      841 atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt ttatgaatcg
      901 caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg cagttacaga
      961 tttactggga atagtcccct atgaacttta taactcccac cgtgactttc ttaatttaaa
     1021 agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct ataataaatc
     1081 ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga gtttttgata
     1141 tataactaca gacagctact ataactttca tctatttatt cacagcgcga atgaaaaata
     1201 aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac tgttcaccct
     1261 gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc gctcaatatt
     1321 ctggaagctg cacgagagcg tattacctgg acactcaaca ccctaccccg cgtttgtgtc
     1381 tcattttctg gcggcaaaaa ctctggttta atgctgcatc tgacagccga acttgcccga
     1441 caaatgggca aaaaaatctg cgttttgttt atcgactggg aggcgcaatt ctcttgcacc
     1501 attaactatg ttcagtccct gcgcgagttg tacaccgatg tcatcgaaga gttttactgg
     1561 gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat accaacccga atggcagtgc
     1621 tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag atgcgataac cgatcctgac
     1681 tttttctgct tttaccagcc aggcatgacc ttcgaacaat ttgtacgtga gtttgccgaa
     1741 tggttttcac aaaaacgtcc ggtggcgatg atgatcggca tccgtgcgga tgagtcctac
     1801 aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgacga taaaccatgg
     1861 actacggccg caccaggcgg tcatagctgg tacatttacc ccatttacga ctggaaagtg
     1921 gctgatatct ggacctggta tgcaaatcat caaaatctct gcaacccact gtataaccta
     1981 atgtatcagg caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt tggcccggag
     2041 caacgacaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc tgctatgtgc
     2101 gcacgagtca gcggggtaaa aagtggcggc atttacgccg gacatgataa tcatttctat
     2161 gggcatcgga aaatcctcaa gccagaacat ttagactggc aagaatatgc attattgctg
     2221 ctcaatagca tgccggaaaa aacagctgag cattaccgca ataaaatcgc catttatttg
     2281 cactggtatc agaaaaaagg catcgaggtt ccacaaaccc agcaagggga cattggcgcg
     2341 aaagatatcc cctcctggcg gcggatatgc aaagttttac tcaataacga ttactggtgt
     2401 cgggcattat catttagtcc tacgaaagcg aagaactatc agcgttataa cgaacggata
     2461 aaaggaaaac gtcaggaatg ggggatacta tgcaacaacg attaacgcag gatttaacgc
     2521 aatttctcgc cagcttgccg gaagatgatc gcatcaaagc gattaatgaa atccgcatgg
     2581 cgatccatca ggttagcccc tttcgtgaag agccagtgga ttgcgttctg tgggttaaaa
     2641 acagccaact tatgcctaat gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac
     2701 tgctgcaaaa atccattgaa atagatggtt ttacccaacc cattgtggtc acacatacgg
     2761 ataaaaatgc catggaaatc gtggatggtt ttcaccgcca tgaaattggt aaaggctcat
     2821 catcattaaa attacgctta aaaggttatt tgccggtgat ctgtctggaa ggtacgcgta
     2881 atcaacgcat cgccgcgact attcgtcata atcgcgctcg tggacgccat caaatcaccg
     2941 ccatgtcgga aatcgtccgt gaacttagcc agttgggatg ggacgataat aaaattggca
     3001 aagagctggg aatggacagc gacgaagtgc tgcgcctgaa gcaaattaac ggcctgcaag
     3061 agctgtttgc cgaccgtcaa tattcccgcg cctggacggt taaataacta aagctggcgc
     3121 aggcgttcag ctgctgccag caacgtcgat tccttcttgg caaaacagag acgaatcagt
     3181 ttatgtggga agggatcggc gcaaaacacc gacagcggaa tcgccgctac gccgtgctcc
     3241 tgcgtcagcc actggcaaaa ctcaacatca tccagggtag aaaccgcgct gtaatccacc
     3301 agcaaaaagt atgtaccttc acacggtaaa atctccagcc ggctttcatt taaggcattt
     3361 accagaatat cgcgcttctg gcgataaaag tccggtaacg caagataatg ctcaggttct
     3421 gcacgtagca tatcagcaag cgccagctgt gccggggtat tcaccgaaaa ggtcagatac
     3481 tgatgtacct tgcgaatttc ggcgctgatg ggcgctggcg caacacaata acccactttc
     3541 cagccggtca tatgataggt cttgccaaat gaagaaaccg ccactgcccg ctcacgcagc
     3601 tgcgaatgcg ccagcacact ggcatggccc tgttgtgaaa agttgatgtg ctcgtagact
     3661 tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc ggcgaaatca
     3721 gcctgctgcc agacagttgc actggagtta tgcggagtgt tgaggatcac cagtctggtg
     3781 cgctcgctta ataatgcggc aaattcctgc cagtcaacgc gaaaatgcgg tggttgcagt
     3841 gccatacgct tcactattcc cccagaaagc gcgatggcgg gggcgtaact gtcatagctg
     3901 ggatcaaaac aaatcacttc atcgccattg cgcaccagtg cggtaatcgc cgcgtataac
     3961 gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catctggttg atagccatac
     4021 aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt catgggcgcg
     4081 tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata gcgcggacca
     4141 tcaaaatcag gaaagccttg cgacaggtta atcgcctggt gttgctgcgc cagcgcgctc
     4201 atctgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat cagagggtta
     4261 tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa cacgatgcta
     4321 gtatttggca atcaagacgt ttagatgtct aaataaaaca ataaggacaa cacaacatgc
     4381 ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat ccaggaagtt
     4441 tcaatcacct gaacgatttt ttcactgatg aacaactttc tcgcgcggtg tggatctacg
     4501 gcaaacgcgc cattgctgcg gcgcaaacca aacttccgcc agcgtttgga cttccagggg
     4561 caaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtacagcaa ctggcggctg
     4621 agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcactg ctcgacaccg
     4681 cgaaagccct cgcccgccgt ctcggtctgc catttgttgc cgttccgacg atcgccgcta
     4741 cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag gcgctgcatt
     4801 atgagatttt cgacgacgcc aattttatgg tgctggtgga accagagatt atcctcaacg
     4861 cgccgcaaca atatctgctg gcggggatcg gtgacacgct ggcgaaatgg tatgaagcgg
     4921 tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcgactgggg atcaataatg
     4981 cgcaggccat tcgcgacgta ttgttaaaca gtagcgaaca ggcgctgagc gatcagcaaa
     5041 atcaacagtt aacgcaatca ttttgcgatg tggtggatgc tattattgct ggtggtggga
     5101 tggtcggtgg tctgggcgat cgttttacgc gtgtggcggc ggctcatgcc gtgcataacg
     5161 gtctgactgt cctgccgcaa accgagaaat ttctccacgg caccaaagtc gcttatggca
     5221 ttctggtaca aagcgccttg ctaggtcagg atgatgtgct ggcgcaatta accggggcgt
     5281 atcagcgttt tcatctgccg accacgctgg cggagctgga aatggatatc aataatcatg
     5341 cggagatcga caaagtgatt acccacaccc tgcgtccggt ggaatacatt cattacctgc
     5401 cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaaagtggaa tcgtttaaag
     5461 cctgacgtgc aaatttagca gcagcgcgcg ccgccttttc caccgtagcg cgcgtcctgc
     5521 cgctcacgga aaaactcttc gtaggtcatc ggcgtttgat cgggatggtt aacccgcata
     5581 tgttcgacat agttgtcgta atcaggcata ccaatcatca gcttcgccgc ctgacctaaa
     5641 tattttccgg cttttgccag tgaatcaaac atcttggcct ccgctaacca tgttgctgaa
     5701 agagtgccgg atgcggcgtg aacgccttat ccggcctaca aatgctatca acgcgctcgg
     5761 tcagtcccct cgccccatcg gggagagggt tagggtgagg aggaaccctc ggcactatgc
     5821 caacattcgc caacatcccc cctcactctg actttagtgt gcgccttttg cctgcgccac
     5881 gatctcctcg acattttccg gcattggctc atacggcgtt tctttcgccg ttggcttcgg
     5941 atctttcaat gccgccagtg ccgacttaat cgagaacagc gccagaacca ccacgaccac
     6001 cataaagaag atggttaacc cggcatccag acggttgttg aacaccagtt gcgccagctg
     6061 tgactcagta tactgcgacg gaatattgcc gctgtcgatc attgcctgga acttattagc
     6121 aatggccagg aagccgactt tcgcatccgg gctaaacgct ttctgccagc ctgcggtcag
     6181 ggtacaaatc agcagccagg ccgttggtac cagcgccacc caggcgtaac gttgacgttt
     6241 catcttgaac aacaccacgg cacagagcat cagcgccatc cctgccagca tctggttggc
     6301 aataccaaac agcggccaca gagtgttaat gccgcccagc ggatcgacca cgccctgatg
     6361 gaggaagtag ccccacgcca gtacgcacag cgctgttgcc agcaggttag caggcagtga
     6421 atcggtccgc ttcaggccag gagacaccac gcccagcaga tcctgcaaca taaagcgcgc
     6481 agcacgcgta cccgcatcta ccgccgtcag aataaacaga gcttcaaaca gaatggcgaa
     6541 gtgataccag aacgccacat ccatcatacc acccagcgca ccgtgcagga tgtaagccat
     6601 ccccaccgcc agcgtcggcg caccgcccgc acgggaaatg atcgactgtt cacccacttc
     6661 gctggcaatc tggttgagcg tatccggagt aatcgcaaag ccccagctac tcaccacctg
     6721 cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc agcaccgcca tcgggctgtt
     6781 catggcgaag tacacgcccg gatcgatgat acaggcggaa accagtgcca taatcgccac
     6841 gaaggattcc attaacattc caccgtagcc gataaagcac gcctgccctt cgttcgccag
     6901 catcttcggc gtggtgccgg aagagatcag cgcatggaag cctgacaccg cgccacaggc
     6961 gatggtgata aacaggaacg ggaacagatt accggtccat accgggccag tgccatcgac
     7021 aaatttggtc agcgcaggca tggtcagcgt cggacgcata atcaaaatgc ctaccgccag
     7081 accaacgata gtcccgattt tcaggaaggt agagaggtag tcacgcgggg ccagcagtaa
     7141 ccacaccggc agcaccgccg ccacaaaacc gtagcccacc agcatccagg tcagctgcac
     7201 gccggtaaag tcaaagtacg gtgcccaggt cgggctttct gccacccagc cgccagagat
     7261 aatggcaaaa ataaggaaca ccaggccaat cactgacact tcgccaatac gccccggacg
     7321 cagatagcgc aggtagattc ccataaaaat cgccagcgga atggtgaacg caacggtata
     7381 cgttccccac gggctatgag tcagggcttt caccacgatc atcgccagta ctgcaaggat
     7441 aatgaccatg atcataaagc aggctaccag cgcaatcacc ccggcagttg gccccatctc
     7501 ttctttgacc agctcaccca gcgagcgacc gtcacggcgc gtagaaacaa acagcaccat
     7561 gaaatcctgc accgcaccgg cgagaaccac cccggcgagc agccagatca tccccggcag
     7621 gtagcccatt tgcgctgcca gcaccggccc caccagcggc cctgctccgg caatggccgc
     7681 aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc cgtcgttatg
     7741 gcgcaccgct ggcgtcatac gcgtcgggtc aaccgccagc acatttttgg cgatatacag
     7801 cccataaaaa cggtaagcga tcagatagat acagaccgac gccaccacaa tccacagcgc
     7861 gttgatctgt tccccacgat ttaaagcaat gtatcccaga gcaaatgctc ccattacaga
     7921 gagcactgtc cagacgaggt atttccctga tttgttcata gttgttatcc gtgtgcgtgt
     7981 ccatagagat gttacatttt gtttctataa catctttgta cgatttaaca acacggtaac
     8041 catgtaaatc ggagttgaaa ccaatattta acttaacaat gttaactcga tcactccgtt
     8101 aaccgagttt tcctgcaatc tcatttaatt ctgtcgggct catcccaaaa ctgccgtacc
     8161 cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa acgatgattt cccagctctg
     8221 attttgccgc ccaagatgca gcggctggca gacgccgcgt accttgcctt cagacaccgg
     8281 gcgatgatgg gtggcgttaa gttccgtacc caccacacac tgcccgtcac gggtcatcat
     8341 aaatccggcc atcgatccca gcgtttcagc cagcgccgcc gacgcgccgc catgcagcag
     8401 gccaaacggc tgatgagtac gggtatcaac cggcatttcg gcttccagca catcatctcc
     8461 cagacgggta tacacaattc ccagatgcgc caccattgtg ttatcgctgg tggcgttcag
     8521 ttcgtcgagc gttaaatggc gtttccagat catgcttatg cccccagcgt tgagccgcca
     8581 tcgaccacaa tatcctgtag ggtgatatgg ctggcgaggt cagaggcgag gaacaaaatc
     8641 gtgttggcga tctcttgtgg acgggcgatt ttccccagcg gaatgccgag tttaaactgc
     8701 tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag cgtgcgttgc
     8761 atatcggtgt cggtggagcc aggcgaaacc acattacagc gcacgccgct aaccgccagt
     8821 tccagcccga cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc ataagcactc
     8881 ataccaatac gcggcgtgtg tgcggcgtcg gacgccacag tgacaatcgc cccgccccgc
     8941 tgacggcgaa actggttcat ggtttgctga aacaggttaa acgcaccgcc gacgttaacc
     9001 gcaaaagtct gctgccagtc ctctttactg agctgatcgg tcgcgcccat gcgcaaaatt
     9061 cccgccgcat tgaccagcac gtccagtcgc tccgtttcag ctaacagtcg ctgacatacc
     9121 tgcgcgacct gcgcagcatc ggcaacatcc atcacttcgg tcgcaaaggg atattgctcc
     9181 tgagcgaacg cttgatcaaa accggtaact ttcgctcccg cttcaacaaa cgccagcgcc
     9241 gtggcgtagc cgataccttt acctgcgccg gttacccaga catttttacc gctgaaatcc
     9301 attatttcac ctcgcgggag agtagcttcc accaggcgtc gatggtcggg tttttcgcca
     9361 gcatgacaaa gtcgatatcg ccatgcactt tgcgccagcg cgccgccagc gccatcatgc
     9421 gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca tcggactcgt
     9481 ccagcaacgg caggataacc tcacgcagcg ccgctttcct ggcggggaca ggtgctggca
     9541 gtaatccttc agtcatcacc acccggccag accgcccggc cacatatttc agcgacatca
     9601 aatgctcgtc acggctgaaa tcggccagcg catccgccac cataaacggt ttaatatcgc
     9661 gcataaatgc gtcggttgcg gtggtcatac agccaatgtg ggcatatacc ccggtaataa
     9721 tcagctggtt acgtccactc tctttgagca tttgctccag cggagaacga tgaaacgcgc
     9781 tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg cgtcaggcga tccaccacct
     9841 tttgctgttc cggcgaacgg gtcaggcccg gcccccacat atcattcaac agcgcccgat
     9901 cttcatcgct ctgctctttc ggctgggcgg tgtaataaac cgggatattg tgctgtttgc
     9961 agtagtcgcg cagcgcagca atattcgcga tcacctgctc catcatcggg cagttctcgc
    10021 cccagaagct gacaaaatag tcctgcatat catggattaa caacgcggca cgttgcggtt
    10081 caaaggccca gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt gcgtaagcct
    10141 gtaattttgg aatagccatc gtgttctctc cttcaggctg atgcgcgtga cgccagccac
    10201 tgacgtaatt gttttttatc gactttcccg accgccgtca gcggaagtga atccacacac
    10261 tccacgcgat ccggtaattt aaattcggca ataccctgtt cacgcaggaa acgacgcacc
    10321 tgcaccgcgc gcagcggctc ttttaccacc agataagcac agcttttttc gcccatcagc
    10381 tcatcttcca tgctcaccag tgcggcatag atcaccgccg gatggcgcag cagcaggttt
    10441 tcgatctctt cggcagcgat cttctcgccg ccacggttga tctgatcttt ctcgcgcccc
    10501 tgcacggtga tgtaaccctc tggatcaata gagatcagat cgccggaaca gtaaaaaccg
    10561 ttggcatcaa aggcactggc attgtgctgc ggacttttgt aataaccgcg gaaggtgtac
    10621 ggcccgcgcg tcatcaggcg tccaacttcc ccttgcggca gtggatttcc ttcagcatca
    10681 gcaacccata cttcatcgtc cggacacatt gggtaaccct gggtatggat aattttctcc
    10741 gcgctatcat caagtcgggt gtagttcacc agcccttccg ccatgccaaa cacctgctgc
    10801 aactggcagc caatctcagc ggaaatacgc gccgcaaggg tggcagaaag acgtgcgccg
    10861 ccgacctgta acagtttcag cgaggcaagc tgcgcccggc tttcgccttc agccagcgcc
    10921 tgcaaccaca ggctgactgc cggcggcacc agcgcggtaa cgttaacctg atgttttgca
    10981 atcaacggga agcaaagcgt ggcgctggga tcggccgcca gaacaaccgt tcctccggca
    11041 agaaagacgc ccagcgatcc tggcgaactc atagcgtagt tatgagccgc cgggatcgcg
    11101 cacaggtagc gtgtctgttg tgtgaactga caaatctcga cgctacgacg cacgctgtag
    11161 tagtagtcgt tatgagtgcg cgggatcagc ttcggtgtac cggtggtgcc gccggaaagc
    11221 tggaaatagg ccacttcatc agcaggtgat ggcgtagcag taaaaccgtc tgccggatgg
    11281 ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttga gcagttgcac cacgcgaatg
    11341 gaggaatgtt ctgtgacgaa agtattgagg aaatcatccc cgctaaacag cgcatgttgg
    11401 cgatcggcaa tcagtaacgc cggttcaatc tggctggcat aggcgttcag ttcactacgc
    11461 tgatggctga acaacgccag caccggcgca acgcccagtt tcagcagtgc gaaaaaggta
    11521 atgtaaagtt cagcgacgtt acccagttgt accagcgcgg tttcaccggg tttaatgccc
    11581 tgacggcgta aactacacgc gaggttatcc gccgcctgat tcagctcccg gtaactcaac
    11641 tgtcgctcgc cgtcgataac cgcgatgctg tcactcgcag cgtggcgagt cagaatgtcg
    11701 gtcagcggca aatcctgcca gtagcctttt tcccgatagc gacgggcaaa ctcttccggc
    11761 cagcgggtga atggaatgct catcctcgct ccttaatgca atccaaaaac gttcaacatg
    11821 gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc aggcacaatc
    11881 cccgctccgg caaacagacg cacctgattt tcccgcagct tcgcgcagcg gatggtcacc
    11941 acccattcgc cgttaccttc gctgtcacac caacccacaa tgccgccaaa cagttcgcgg
    12001 tcaaacggct ccagttcagc aataacctgg gtcgcggcct gatgcgggaa accgctcagc
    12061 gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt tttcttgcga attcgcttta
    12121 ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg taatcaattg tggagaggag
    12181 ggaacgtgta actcactact gcgttcgcgc agtacctctt tcatcgcctg agtcaccagt
    12241 tcatgttcat ggcgatcttt ttctgacgcc agcagacgat tacccgcttc gcgatcgagc
    12301 acttcatccg gctgacgacg cgcggaaccg gctaacggaa tggagctaaa acgctcgccg
    12361 tctttacgta gcagcagttc cgggctggcc cccagcagga cgccaccatc agccagcggg
    12421 acatggaagt tgtaactaac cgggttttgc gcaatcaacc gttccagcaa tacgccacta
    12481 tcaatggcgg cgtcagtggt gatatcaatc aatcgtgaca acaccacttt gtcgacctgc
    12541 ggcgtggcgg taagtgcggc ggcgcgggca accatctgtt caaacgtggt ttgttcagga
    12601 attgcctggc gttccaccac gttcagcgac tggctgcggg taaaacagtg ggctgaggtc
    12661 tgtttttcct gacgggagaa cgactgccag gattcgggaa tatacagcga cgaaggctga
    12721 cgtggatcga agggaatcgc cccgaccatc accggatttt tgatgccctg cgctttggca
    12781 tcggcaaaca gcgcggcgag tttttgctgg aagggactgt cgggcgaatc cccgttcaca
    12841 gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta cggcgacata
    12901 aagaaaaagc gattgggcgc aagtgttgcc atggtctgct gtacttcctc agccagtgac
    12961 gtatccatat catcctccac aaaatgataa aggctttaat aatgattatc atttatattt
    13021 tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa cgcaatctgc
    13081 aactcggggt tgcatctggt tgtggttaca atgaaaatga gaagcattat tgatgaattc
    13141 gcataagcgc aatgtgatgt cctgcgccgt tttgccccct ctcccttcca gggtgagggc
    13201 tggggtgagg gttaatgttc gcaccagtgc tggctgttcc cctcacccta accctctccc
    13261 caaaggggcg aggggacgga ttgtgcgctt tgtcgaattt gtcattacgc ccttaacctt
    13321 attaataaca ggaagctgac ttgtgagact cgccccgctc taccgcaacg cccttctatt
    13381 aacaggactt ttgctttcag gaatagccgt agttcaggcc gccgactggc cgcgtcagat
    13441 tactgacagc cggggcactc ataccctgga aagccagccg cagcgtattg tttccaccag
    13501 cgtcaccctg accggatcac tgctggcgat tgatgctccg gtgatcgcca gcggcgcgac
    13561 cacgccgaat aaccgcgtcg cagatgacca gggcttttta cgccagtgga gcaaggtggc
    13621 gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agcgccgaag ccgttgccgc
    13681 gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggcgc tggcgctgta
    13741 tgatcagctt tccaccatcg ccccgacatt aatcatcaat tacgacgaca aaagctggca
    13801 gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg cagagcggat
    13861 tgcacagttt gataagcaac tggcagcggc gaaagagcaa atcaaattac cgccgcagcc
    13921 ggtcactgcc attgtctata ccgccgctgc acacagtgcc aatctctgga cgccggaatc
    13981 agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaagttgc ccgcagggtt
    14041 aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg gggaaaatct
    14101 ggctgctggg ttaaacggtg aatcgctatt cctgttcgcc ggtgatcaga aagacgccga
    14161 tgcgatttat gccaatccac tgctcgcgca cctgcctgca gtacaaaaca agcaggttta
    14221 tacgctggga accgagacgt tccgcctgga ttactacagc gccatgcaag tgctggacag
    14281 acttaattca ctgttaaaaa aagattgata aaacatacaa ttagagcact aataatcccc
    14341 tcgccccttt ggggagaggg ttagggtgag gggaaaatgc acctttagct aactgtcaga
    14401 cgctgtcacc tgcggcggcg tctggcgaaa acgtcgcaac tccaccagca ccagcaataa
    14461 caacacgccg ataatcaaca aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat
    14521 catcgcgccc aaaccaccca acagcgccgc gcctatggca tcgcccgtca cgttctgcgc
    14581 cgtccacaaa ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt
    14641 atattgcagc aacgaactaa ccgcgctcag ccagccgaac agcgccagac aaaccacgcc
    14701 taaaatccac atcggcatca ggccaaacag accaatggcg aggaacgatc ccagcgtgga
    14761 gagcagcatc aataaccctg gtcgcgcact atgtgccagc ttcccgctgg ttaacgcacc
    14821 aatagccgca ccgagcggga tcgccgcata gagaaaacca atctgtgccg ccgacatctg
    14881 ccagttgtca gccagcgccg gatacagtac ccgcaccgcg ctcgccatcg tcaataaacc
    14941 acccagcaac gcaatccctc ctaccagcgg gctggcgagc agaaaacgaa atcctgccag
    15001 taatgatttc aacggatgct cgcgcggctg cggtggcggt ggcaacgccg gaaggcttaa
    15061 caacggtagc aaggtaataa acgtgcccgc cgccgccagc ccgtagttcc aggctacgcc
    15121 gccggtcgcc agcaataaac cgccaatcat gggcgaaatc accgacccca gacgcacggt
    15181 caacatggtg atcgccccgg cctgcattaa gttttcacgc cctaccagtg caggtgttgc
    15241 cgccagtagc gccgtaacac caagtgatgc gaaaaaacca tcccataaac caagtaaata
    15301 gattgccagc aatgacggct ccggcagcag cgcgttcagg cacagtccaa tgaagccaat
    15361 gccacaggtg ccgcgcgcca gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac
    15421 accgccaacc atcaggccaa caaacatcgc gccgccggtc agcgtcaccg aaagccccac
    15481 ctgccaggta gaatgcgtca tcatctggat ctgcaccggc accgcgacgc cgagcaaacc
    15541 cagagacaca attgagataa aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt
    15601 caacaggctg aggttaagca gccaggattg tttattcatt acaatgcctt gccatcaaat
    15661 attttaaata cctattctta ggctgcacat gctaacatat ccaaataaga tcgataacga
    15721 taattaattt cattatcatg gaagttcgta tgtctggttc tgttgccgtg acacgcgcca
    15781 ttgccgtgcc cggattgctg ttattactga ttatcgcgac ggcattaagc ctgctcattg
    15841 gggcaaaatc actccccgcg tcagtagtgc tggaggcctt ctcaggcacc tgccagagcg
    15901 ccgactgcac catcgtgctc gacgcgcgac tgccgcgcac ccttgccggt ttacttgcag
    15961 gcggtgcgct tgggcttgct ggggcgttaa tgcaaaccct cacccgaaat ccacttgccg
    16021 accccggctt gcttggcgtg aacgccggag ccagctttgc cattgtgctg ggtgcggcgc
    16081 tgtttggtta ctcatccgcg caggaacaac tggcgatggc cttcgccggg gcgctggtgg
    16141 cctcattgat tgttgccttt atcggcagtc agggcggcgg gcagttaagt ccggtgcgtt
    16201 taaccctggc gggcgtggcg ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc
    16261 tgcttaatcc tgacgtctac gatcaactgc gtttctggca agccggttcg ctggatattc
    16321 gcaatctaca taccttaaaa gtggtgctga tcccggttct gatcgccgga gcaactgcgc
    16381 tattactgag tcgcgcgctg aacagtttga gcctcggcag tgacaccgcg acggcgctgg
    16441 gcagtcgcgt ggcgcgcaca cagttgattg gtctgctggc gattaccgtg ctttgtggta
    16501 gtgcgacggc ggtagttggc ccgattgcct ttatcggcct gatgatgccg cacatggcgc
    16561 gctggctggt aggtgccgat catcgctggt cgctgcccgt cacgctactt gctacccctg
    16621 ccctgctgct gtttgccgat atcatcgggc gggtgattgt gcccggcgaa ctgcgcgttt
    16681 ctgtggtcag tgcgtttatt ggtgcaccgg tgctgatctt cctcgtgcga cgtaaaacgc
    16741 gaggtggtgc atgatttacg tctctcgccg attactcatc acctgtttgc tgctgatttc
    16801 cgcctgtgtg gttgcaggta tctggggatt acgcagcggt gccgtcacgc tggaaatctc
    16861 gcaggtattc gccgcgttga tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga
    16921 atggcgttta ccacgcgtgc tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg
    16981 cgcgattttt cagtcgctga tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa
    17041 caccggggcg tggagcggcg tgctggtggc gatggtgctg tttggtcagg acctgacggc
    17101 tatcgcgctg gcagcaatgg tgggcggcat tgtcacttct ctgttggtct ggctgctcgc
    17161 ctggcgtaac ggtatcgaca cctttcggtt gattattatc ggcatcggcg ttcgcgccat
    17221 gctggtggcc tttaatactt ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc
    17281 gggtttgtgg aatgccggat cgctcaatgg cctgacgtgg gcaaaaactt cgccttccgc
    17341 acccatcatc atattgatgc tcattgccgc cgccttgctg gtgcggcgaa tgcgcttgct
    17401 ggagatgggc gatgataccg cctgcgcgct gggcgtcagc gtcgagcgtt cgcgtctgtt
    17461 aatgatgctg gttgcagtgg tgcttaccgc tgcggcaacg gcgcttgccg ggccgatttc
    17521 ctttattgct ttagtcgcgc cgcacattgc ccgacgcatt agcggcaccg ctcgctgggg
    17581 gctgacccag gcggcgctgt gcggagcgct gttactgctg gcggccgatc tctgcgccca
    17641 acaactgttt atgccgtatc aacttccggt tggcgtcgtt accgtcagcc tcggcggtat
    17701 ttaccttatc gtcttgttaa ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt
    17761 ttgcgcggcg aacagttaac cctgggatat ggcaaatata ccgttgcgga aaatctgact
    17821 gtagaaattc ctgatggtca cttcacggca attatcggac caaatggctg tggtaaatcc
    17881 acgttactgc gtaccttaag ccgcctgatg acgcctgctc acgggcatgt ctggctggat
    17941 ggcgagcaca ttcaacatta cgccagtaaa gaagttgcac gccggattgg tctgttggcg
    18001 caaaatgcta ccacgccggg cgatatcacc gtgcaggagc tggtggcgcg tggacgttat
    18061 ccgcatcaac cgctgtttac ccgctggcgc aaagaggatg aagaagcggt aacgaaagcg
    18121 atgcaggcca cgagaataac tcatctggca gatcaaagcg tggataccct ttctggcgga
    18181 caacgccagc gagcgtggat cgcgatggtg ctagcccagg aaacggcaat tatgctgctc
    18241 gacgaaccca cgacctggct ggatatcagt catcagattg atctactgga actgttaagc
    18301 gaactgaacc gcgagaaagg ctataccctg gcggcggtgc tgcacgatct taatcaggcc
    18361 tgtcgttacg ccagccattt gattgcattg cgggaaggga aaattgttgc tcagggagcg
    18421 ccgaaggaga ttgtcactgc tgaactgatt gagcgcattt atggtctgcg atgcatgatc
    18481 attgacgatc cggtagccgg aacgccgctt gtggtgccgc tcggacgaac ggcaccttca
    18541 accgcaaata gttaaactaa gtggtctgcc atcatggcat cctgttttct ggatgccatc
    18601 gcatagcgca ataacacgct accacaagcc accatcccgc cgatcagcgc ggaaaggatc
    18661 acaataatcg ccttacccgg accgtctttt ttcactggca atgacgggct taactgatat
    18721 ttaaacggcg taaaattcac atcgttgaca tttgcttttg ttaattgctc gacaagatac
    18781 tgccgattac gtaattcacc gttcagttcc gcaacgtcag taaccgcttt ttcaatttcc
    18841 agtttgcgtt caataccgtc tgcgccgaga gaaatagaga aatcggggtc atctttaacg
    18901 gcctgaccat tactgtatac gggcttttta attcctgccg cgttggcaat gtcgagtgaa
    18961 taattgaggc gctgaatgtt tgcatcaagt tgatttttcg ttttaatgcg atcctgagcc
    19021 agtttttctt tttcaaactg ggttttgatc tccagtttat tacggacgtt ttctatcgac
    19081 tctttcacca ccaacgcaga gatataatcg atatacccgc tcaaaacggt ctgcgcctct
    19141 ttactggttg gcgcggtaaa acttagcgtc caggaggtat acagtgacgg ttcatctttt
    19201 ttcttactgg cattgtcatc aaccgctttc attttttcgc tcaatgcaac aatagcgcga
    19261 tgcaaatcca gttcatcgat tttcgcctct tttaattggt ccatcacata aggtgatgaa
    19321 cgcaggtact cttccagcaa gctaacagac tgaaacttct tgataaacag gttaaatgca
    19381 ccgccacggt cgatattaac atcgagatca agaacgcgga gtttggtaaa ggttttctcc
    19441 aggtcctgcc actgaatggc ttcggcggga gtaataacgg ctgagctggt ccatttttgc
    19501 ggcaggatga aagagatcag taaacctgcg caggcaaatg caaaaacgac cgccatgacc
    19561 gtttttttgg cccgccataa aacttcgatt agattaagta aatcaatttc attattactg
    19621 ggcgacgcca gaggatactc agaaaaatga gcgtcacttc cctgtttaat attcaatgat
    19681 gacatggggt ataacctgaa aatgagtcca ataggggaaa atctccacgg cgctcacttt
    19741 accagttggt gagaattatc cgaagctgaa gtttgtaaat tccttccact gaatgcaata
    19801 ttcactctat ttaagagaaa tattcatcaa acctataaat caaacaaata aaaaacccat
    19861 aattattttc tgtaattatg ggtttataga taatattaat ttacctgttt agcgttgcgc
    19921 gaataatcgg cccaattttt acaaacgccc ctggagagat aatatccaca tgcgcacaat
    19981 cctgacgata gatatccagc tccgcaatcc acggcgacca ggcgcgttca ggactcatac
    20041 cttcctgaag cgtgcgttca gcaacaaaca acgtcgcttt accgtcaaac ggtacgctat
    20101 gagcagtcgt cagcaggcgc acagcatcag cgtagttgcc ttcaatggtg gtaaacaact
    20161 ccgttgaagt acttccctgc tgtgccgcca ggaaggcctc gcgctcgcgg ttaatctccg
    20221 ccagcacttc cgggtccaga ccattagctt ctttttcctg ccagttttgc gtttctggcg
    20281 gccaggtatc cagcaagcca agaaatgcca cctgttcgcc acgggcacgc agtcgcgccg
    20341 caataccctg cgccagcgta ccgccaaggg aataccccag caggtaataa gggccgtgcg
    20401 gttgttgctc aagtaacgtt gccagatgcg cttcgcagac ttcatccagg tttgtcgctg
    20461 tctgcatggg tccatgaggg cgcggcgact gaatgccaat aatcgaccat tgtggatcga
    20521 gataacgcga gagtacgcta aactgccagg caaaaccgga cgccggatgg aaacaaaaca
    20581 gcgtcgggcc attaccttca cgcaacggca gaatggtttc gaatcccatg cgccgggagc
    20641 tgtcctcttc accatcaata atcgttgcca gtttggcgac ggttgacgcg accatcacct
    20701 gccccggcgt cacctgacga gcaaactgtc gacttaactg cgctgccagt ttcattgcca
    20761 gtagcgaatg accgcccagc gcaaagaaat cagcatcggc atcctgcacg tcacaaccca
    20821 gcaacgacgc gaatgccgcg gcgataatcg tttcactgcc cgctttcggt gcacgtcccg
    20881 acgcttgtgt cttcagttca ggcaacggta aggctttgcg atccagcttg ccgttggcgc
    20941 taagaggtaa ctgtggaagt tgcagcagaa ccaccggtac catatgtggt ggcaatgttt
    21001 cacgaagctg cgcctgcaat gcgctggtat ccaacggcag gcccgattgc gacaccagat
    21061 agcccaccaa ctgacgcgca tcaccaccag tggctgccgc ctggttaatc acacaggcgt
    21121 gggtaacggc ttgttcgaca tccggcagcg cctgcatcac gcgatcgatt tcgcccagtt
    21181 cgatacgctg cccgcgaatt tttagctgat cgtcactgcg cccgaggtac tccaccgcgc
    21241 cgttatccag ccagcgggca acgtctccgg tacggtacat ccgttcgcca ggagcaaaag
    21301 gatcggcaat aaagcggctg gcggtcagat cggggcgtcc gagatagccc tgcgccagtt
    21361 gaatgccggt aagatagaga tcacccgcca cacccggcgg caccggatgc atcatcgcat
    21421 caagaatacg cagccccatg ttccacaccg gataaccaat cggcacactg ctgccgcgca
    21481 cctgtgccag ttcctcgcca aaagccggat accagctgac atctaccgcc gcttccgtcg
    21541 ggccatacag attatgtaac ggcgcaccgg ttaactgttg ccattcacgg cataaatcag
    21601 ccggtaaggc ttcaccacta cagaaaacct gtttcaacgt cgcgcaactc tggcgagcgg
    21661 tttgcggagt cagcgaggca acaaatgccg ccagcatcga cggcacaaag tgcgtggtcg
    21721 ttacgccata ttcggcaaag aattgctgca tagcgagcgg gtcacggtgc gcttccggtt
    21781 cagccatcac cagttttgcc cctgcgataa acggccagaa aaactcccac accgagacat
    21841 caaaactgca cggcgttttt tgggcaacga catcttcgcc tgtaagcggg tagtgatttt
    21901 gcatccataa caggcggtta acgatagccg tctgcccgac cattacccct ttcggcctgc
    21961 cggtggagcc agaggtaaag atgatataag ccgtgtggtg cggttgtgaa agttgcagcg
    22021 gcgcactgcc ctggggggta agcggggcgt tatagcaaag gcttgttaaa ttgggaacat
    22081 cgctaaagcg cggcagttga tcgtcggtgg taattaacag cgacggacgc gcatcttcca
    22141 gcatcatttt caggcgatcg tccggatagc cggtatccag cggtagccag gccgcgccag
    22201 cttcaactat tgcatggagt gccagggtca aaaagaccga gcgcggtagt gccaccgcca
    22261 cgctgtcccc tggtttaacg ccgcgctcac gcagcagatt cgccagcgcc accacctgct
    22321 cgcgcatttc ccgatagctg aacaggtaac gcgcatctgc cagcgccgga gcatccggtg
    22381 tttttgccgc ttgttctgcc accagcgcgc tcagcgtggt ttctggaatc tcaacctgag
    22441 tggcgttgat ctgcgccagc tgcgcatact cacctggcag cataatatcg acatcgccgc
    22501 acaacagcgc cggatccgcg gcgaactggg caatcagcat tttcaggcgt tcagcatgct
    22561 ggattaacgt tggctcatcg taacgctgtt tattggcgag gatctcaata ctcaaatcac
    22621 cgtgttcatc cgggaacagg gccagttcaa ggtcattaac cggaccggtt gccagggtat
    22681 gggtttgcgc ctgaacacca ggaatatcca gttggtaatc aaatacctta atattgagta
    22741 ccggaccaaa cagcggttca tcacctgccg ctcgcccgct gtcacggaca atttgttcgg
    22801 catcgtaacg ttgatggcga cgcatttttt tcagttgagc cgccagtcgg gttgccagtt
    22861 ccggcagcgt ttcttgcgcc gcaatgtgaa tacccaacgg taaaacgttg agcacgggtc
    22921 cgctagccgt cagcgccgcc gagcccagtc gacgcataaa gataaatccg gcggcatagt
    22981 ccatgcgatt gcacaatcgc cccagccaca aggctgccag cgcaagggct aaatcggtac
    23041 gctgcacacc tgaaagttgc gtagccagct ggcggaattc cccgtcggta aattccagtt
    23101 tcaggcgcag aatatctgcc gaagcgctgc gccccggtaa aggtgccgga gaaagtgacg
    23161 cgggcggcgg cagttgacga cgctgttctg cccagaatgc cgcatcacgc tgccaggctt
    23221 cgctttcgcg gtattgctgg tactcttcca ctacatcagc gaaaggcgta aacggcgaag
    23281 caggcgttgg ttcgccacgc agccatgtgc agtaaatatt ggcgatctgg cgagtaatgg
    23341 ccgggaaact gaagccatcg accagcaaat ggtgataacg ctgataccag taccagcggt
    23401 tatccgcaac atgtatcagc tgatgaaaga ccagtggttt accgctatcg acgcgcaaat
    23461 cttgttgcaa atccgccagc attaatgcct gcgcagtacc gtgcggatca atattggttc
    23521 gcaggtcgat aatttctggc tgttcgaata tcagcgcatc atcgacccac tgccagactt
    23581 cgccgttatc ttccgtaaaa cgcatccgca acgtatctgc ttgcgctagt ccggcaacca
    23641 ccgcgcgggc cagtaatggc gcatcaacat ctccagttaa ctcaacgtaa tgcgccacgc
    23701 tccaggcgga gggtaaatcc gacagttttt ctgccatcca gatgccgggc tgtgcggcga
    23761 ccaaaggtaa atgctggctc attgtgcctc ctgcaactgg gtgaaattgg ccggtgtcag
    23821 agtacgccag tggacttcca gccactgctg gcaggacgcc tgtgactgcg gctgacacac
    23881 aatgtcccag cctgccgata agaggcattg ttgcggccac agactgaatt gcccctgcgc
    23941 attgcgcaaa atgtaaaacg ctccctgcgg atcatcgaag ggattactga atgccatact
    24001 caactcctgt catggaaaag tggttgccag aggtcgatta acccctgcat caagccaccg
    24061 cgccaacaaa gcgcatcatg tccgccgtca acctgacgcc agaaaatgga ttcttttata
    24121 gggtgcaatt gcgcatacag cgcctgattg gcccgcatga tcatcggctc gcgaataccc
    24181 gcttccagca caatgcgcag accttcggcg ctaacttcac cagcttttag cttttcaagt
    24241 aacacgccct cttgctgccc gtcccgatgc ggccaccagt acgatcctga ctggcttaat
    24301 acacagccaa agcgttcagg ccagtgcagt ccggcataca gcgcggacag cccaccaaaa
    24361 ctctgcccgg cgaccacggt gcgatcggcg cgatcgctaa aaggggcaat agctttcacc
    24421 aggggtaata actcttgctg tactgccagc cagaaatccg cattacacgg cagttcgtgg
    24481 gcgcggtgcg tggtgtcgat agcgtcgatc aacacataca cggcgggagg aagttgctga
    24541 cgatgggtca gcgaagtcag cacaggccag acgggcatac tttgcgccca aaattcgcca
    24601 tcgagcaaaa ctgccagcgg acgttcttct gctgttacat cgccggtggt aaaaatccat
    24661 acacgacgtg aatttttcaa ccgttcactt ttccagataa tttctttggc tgctatttct
    24721 ggcacttgcg gacaatccca tcccggttgc agaggcgctt gcggcatttc gagtgcagaa
    24781 acagcgtgcc ctcgcccgcc tttccagctt tgtggattca gcggatcggc tattgcctgg
    24841 ggtaatagtt ttcgccagcc ttcacgtaat tcgaggcgat cgggggatgg tgcagaaaaa
    24901 atgtcatcgc gttcggtggg aataaagcag tagctgccgc gccagttggc attgagttgt
    24961 gtcgtccact gccagacatc agtacctgca attcgctgca tcgactgggg ctggctgttc
    25021 tgatgatgat cggtcacacc agtgatgtag atccatacgc gctttatcgt cgagtattct
    25081 tcagaacctt ggggatcacg ccaccagaaa gtgacctcaa acatttcgtc attcagacgc
    25141 tgccattccg ggccatgttt cgactgccac cagctctcac ttcctacttt taacgccgtc
    25201 acaccataac cccatgttta ctgtgcaatt tttcattgat tgcagaaata tattgataat
    25261 attattgata accatttgca tttgcaatag cgtaatggcg cgccgtggga agcgcggaca
    25321 tttattaacc aactgcactg cgtgtctttc aggatcaaag gttttcgcgg tagcgggatg
    25381 cgtcgtgttg aggacgacca tgcccgacag ttgcaattcg tggcaaaaat gcaggaataa
    25441 aacaatgaac aagaagattc attccctggc cttgttggtc aatctgggga tttatggggt
    25501 agcgcaggca caagagccga ccgatactcc tgtttcacat gacgatacca ttgtcgttac
    25561 cgccgccgag cagaacttac aggcgcctgg cgtttcgacc atcaccgcag atgaaatccg
    25621 caaaaacccc gttgcccgcg atgtgtcgga gatcatccgt accatgccag gcgttaacct
    25681 gaccggtaac tccaccagtg gtcagcgtgg gaataaccga cagattgata ttcgcggtat
    25741 gggtccggaa aacacgctga ttttgattga cggcaagccg gtaagcagcc gtaactcggt
    25801 gcgtcagggc tggcgtggcg agcgcgatac ccgtggtgat acctcctggg tgccacctga
    25861 aatgattgaa cgtattgaag ttctgcgtgg tccggcagct gcgcgttatg gcaacggcgc
    25921 ggcgggcggc gtggttaaca tcattaccaa aaaaggcagc ggtgagtggc acggctcctg
    25981 ggacgcttat ttcaatgcac cagaacataa agaggaaggt gccactaaac gcaccaactt
    26041 cagcctgacc ggtccgctgg gcgacgaatt cagcttccgc ttgtatggca acctcgacaa
    26101 aacccaggct gacgcgtggg atatcaacca gggccatcag tccgcgcgtg ccggaacgta
    26161 tgccacgacg ttaccagccg ggcgcgaagg ggtgatcaac aaagatatta atggcgtggt
    26221 gcgctgggat ttcgcgccat tgcaatcgct ggaactggaa gcaggttaca gccgccaggg
    26281 caacctgtat gcgggcgaca cccagaacac caactccgat tcctataccc gctcgaaata
    26341 tggcgatgaa accaaccgtc tgtatcgcca gaactactcg ctgacctgga acggcggctg
    26401 ggataacgga gtgaccacca gcaactgggt gcagtatgaa cacacccgta actcgcgtat
    26461 tccggaaggt ctggcaggcg gtaccgaagg gaaatttaac gaaaaagcga cacaggattt
    26521 cgtcgatatc gatcttgatg acgtgatgct gcacagcgaa gttaacctgc cgattgattt
    26581 cctcgttaac caaacgctga cgctgggtac ggagtggaat cagcaacgga tgaaggactt
    26641 gagttccaat acccaggcgc tgaccggggc gaacaccggc ggcgctattg atggtgtgag
    26701 tgccaccgac cgtagcccgt attcaaaagc agaaattttc tcgctgtttg ccgaaaacaa
    26761 catggagctg actgacagca ccatcgtaac gccggggctg cgtttcgatc atcacagtat
    26821 tgtcggcaat aactggagcc cggcgctgaa catatcgcaa ggtttaggcg atgacttcac
    26881 gctcaaaatg ggcattgccc gtgcttataa agcaccgagc ctgtaccaga ctaacccgaa
    26941 ctacattctc tacagtaaag gtcagggctg ctatgccagc gcgggcggct gctatcagca
    27001 aggtaacgat gacctgaaag cagaaaccag catcaacaaa gagattggtc tggagttcaa
    27061 acgcgacggc tggctggcgg gcgtcacctg gttccgtaac gattatcgca ataagattga
    27121 agcgggctat gtggctgtag ggcaaaacgc agtcggcacc gatctctatc agtgggataa
    27181 cgtgccgaaa gcagtggttg aaggtctgga aggatcgtta aacgtaccag ttagcgaaat
    27241 ggtgatgtgg accaataaca tcacttatat gctgaagagt gaaaacaaaa ccacgggcga
    27301 ccgtttgtcg atcatcccgg agtacacgtt gaactcaacg ctgagctggc aggcgcggga
    27361 agatttgtcg atgcaaacga ccttcacctg gtacggtaag cagcagccga aaaaatacaa
    27421 ctacaaaggt cagccagcgg ttggcccgga aaccaaagaa atcagtcctt acagcattgt
    27481 gggtctgagc gcgacctggg atgtgacgaa gaatgtcagt ctgaccggcg gcgtggacaa
    27541 cctgttcgac aaacgtttgt ggcgtgcggg taatgcccag accacgggcg atctggcagg
    27601 ggccaactat atcgcgggtg ccggggcgta tacctataac gagccgggac gtacgtggta
    27661 catgagcgta aacactcact tctgatgcta acgtcagatt gttgacaaag tgcgcgtcgt
    27721 tcatgccgga tgcggcgtga acgccttatc cggcctacaa aaatcttgcc aattcaatat
    27781 attgcaggac catgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcatc
    27841 agtctcgaat atggtcgata tgaaaactac gcatacctcc ctcccctttg ccggacatac
    27901 gctgcatttt gttgagttcg atccggcgaa tttttgtgag caggatttac tctggctgcc
    27961 gcactacgca caattgcaac acgctggacg taaacgtaaa acagagcatt tagccggacg
    28021 gatcgctgct gtttatgcct tacgggaata tggctataaa tgtgtgcccg caatcggcga
    28081 gctacgccaa cctgtctggc ctgcggaggt atacggcagt attagccact gtgggactac
    28141 ggcattagcc gtggtatctc gtcaaccgat tggcattgat atagaagaaa ttttttctgt
    28201 acaaaccgca agagaattga cagacaatat tattacacca gcagaacacg agcgactcgc
    28261 ggactgtggt ttagcctttt ctctggcgct gacactggca ttttccgcca aagagagcgc
    28321 atttaaggca tcggagagac aaactgaagc aggttttctg gattatcaga taattagctg
    28381 gaataaacag caggtcatca ttcatcgtga gaatgagatg tttgctgtgc actggcagat
    28441 aaaagaaaaa atagtcataa ctctgtgcca acacgattaa ttgacaacat ctggtacgat
    28501 tcgcccgcag ccatcactga ctgcgggcga aagtgtaaag caggtgcctt accactctga
    28561 cctgacaacc ggatatgcag ggattgctcc ccgcacaacg gctacttctt cggttcgtaa
    28621 gcgagaacag ccttaaactc aatattacgt tcctttacgg tgaactcaca cagcgagtct
    28681 ccgaccagaa gcgtaaatcc cagcaccgtc aaacacagca ctatgactgc cacaagggca
    28741 tatttcgtca gcatgtttat attgcctcca tgcgaagaag ggactaccat ccgagttgtt
    28801 gaggttcgga gtggcagccc caggattgat agaaatatct cctggggctt catctttcca
    28861 gacctcagag taaacctgaa accggaaagc ttcaggcacc cgccgttatc ttacccttcc
    28921 tcagcaaaat atctgtttat ttatacagta agtcctgttt cggacttctg ttggcaacac
    28981 tcgttattgc tcgtaaaacg aaatcgcccg cagccatcac tgaccgcggg cgaaagtgta
    29041 aagcaggtgc cctaccatcc tgacctgaca accggatatg cggggattac tccccgcaca
    29101 acggctactt cttcggttcg taagcgagaa cagccttaaa ctcaatatta cgttccttca
    29161 ccgtaaactc acacagcgag tctccgacca gaagcgtaaa tcccagcacc gtcaaacaca
    29221 gcactatgac tgccgcaagg gcatatttcg tcagcatgtt tatattgcct ccatgcgaag
    29281 aagggactac catccaaatt gttcgggttt ggagtggcag ccccagggtt gatagaaata
    29341 tctcctgggg cttcatcttt ccagacctca gagtaaacct gaaaccggaa agcttcaggc
    29401 acccgccgtt atcttaccct tcctcaacaa aatggctgtt tatttataca gtaaatcctg
    29461 tttcggactt ctcactcctc atttaatcaa ctgaagctga gcccgttttt tctgcatgcc
    29521 tcaatcgtca aaattcttcg ccatcagcca gctctgccat aacttgccac attgaaaaag
    29581 tagccaaatt gcttcagtca ccatcatcaa aaaaagaaac attccagaac ttccgcatct
    29641 cgcccagccg ctccgacttc tatactgaat agaaaacgcc aacataagag aaacctatgc
    29701 cattacccga ttttcatgtt tctgaacctt ttaccctcgg tattgaactg gaaatgcagg
    29761 tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt gacgcggtta
    29821 aaaataagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg ctggagctgg
    29881 cgacggatgt ttgccgtgat atcaaccagg ctgccgggca attttcagcg atgcagaaag
    29941 tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc acacacccgt
    30001 ttcagaaatg gcagcgtcag gaggtatgcg ataacgagcg ctatcaacga acgctggaaa
    30061 actttggcta tctcatccag caggcgaccg tttttggtca gcatgtccat gttggctgcg
    30121 ccagtggcga tgacgccatt tatttgctgc acggcttgtc acggtttgtg ccgcacttta
    30181 tcgccctttc cgccgcgtcg ccatatatgc aaggaacgga tacgcgtttt gcctcctcac
    30241 gaccgaatat tttttccgcc tttcccgata atggcccgat gccgtgggtc agtaactggc
    30301 aacaatttga agccctgttt cgctgtttga gttacaccac gatgatcgac agcattaaag
    30361 atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt cgggtgatgg
    30421 ataccccgtt aacccttagc cacgcggtaa atatggcggg attaattcag gctaccgccc
    30481 actggttact gacagaacgc ccgttcaaac atcaggagaa agattacctg ctgtataaat
    30541 tcaaccgttt ccaggcctgt cgctatgggc tggaaggcgt cattaccgat ccgcacactg
    30601 gcgatcgtcg accgctaacg gaagacacct tgcgattgct ggaaaaaatc gccccttccg
    30661 cacataaaat tggtgcatcg agcgcgattg aggccctgca tcgccaggtc gtcagcggtc
    30721 tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg tggctcgctg attgggctgg
    30781 tgaaaaagca ttgtgagatc tgggccgatg actaagtagg gaattgcctt acagaagaat
    30841 atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc ctttagaaac
    30901 cttgactacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc tgctgcccgc
    30961 ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc tgatggatct
    31021 tagctagctt tacactttat tgttttgtct gtggttttta gtgctgggcg ctattgagta
    31081 ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa cgtcgattat
    31141 cgccgtactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg tttatggata
    31201 agcaatcact gcacgaaacg gcgaaacgcc tggcccttga gttacccttt gtcgagcttt
    31261 gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaaaatt tttatgctgt
    31321 cgtcggagct gcgcggcgtc ccctttatca atctgaagtc cgatccacaa aaatccctgt
    31381 taaatcagca gatatacccg agcattaagc cagggtatca catgaataaa aagcactgga
    31441 tttcggtgta tcccggcgag gaaatctccg aagcgttact tcgcgatctg atcaacgatt
    31501 cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg cgtccaggct
    31561 aaagcggaaa tctatagcgc atttttctcg cttaccattt ctcgttgaac cttgtaatct
    31621 gctggcacgc aaaattactt tcacatggag tctttatgga tatcatttct gtcgccttaa
    31681 agcgtcattc cactaaggca tttgatgcca gtaaaaaact taccccggaa caggccgagc
    31741 agatcaaaac tctcctgcaa tacagcccat ccagcaccaa ctcccagccg tggcatttta
    31801 ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc ggtaattatg
    31861 tgttcaacga acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt gcaaaaaccg
    31921 cgatggacga tgcctggctg aagctggttg ttgaccagga agatgctgat ggccgctttg
    31981 ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgct gatatgcacc
    32041 gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc aacgtcggta
    32101 acttcctgct cggcgtggcg gctctgggtc tggacgcggt acccatcgaa ggttttgacg
    32161 ccgccatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt ctggtggttg
    32221 ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa tctcgtctgc
    32281 cgcaaaacat caccttaacc gaagtgtaat tctctcttgc cgggcatctg cccggctatt
    32341 tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata ggctgctgtt
    32401 gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag cgggtattga
    32461 gatcgatcac accacctcga tggtgatgat tttcggtatt atttttctga ccgccgtcgt
    32521 ggtgcatatt attttgcatt gggtggtact gcggaccttc gaaaaacgtg ccatcgccag
    32581 ttcacggctt tggttgcaaa tcataaccca gaataaactc ttccaccgtt tagcttttac
    32641 cctgcagggg attatcgtca atattcaggc ggtattctgg ctgcaaaaag gcaccgaagc
    32701 ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc tgctttcagt
    32761 cttctcgttg ctggatgtta ttttgaatct ggcgcagaaa ttcccggcgg catctcagtt
    32821 accgctgaaa gggatatttc aggggattaa actgatcggc gcgatcctgg tcggcatttt
    32881 gatgatctcg ctgctgattg gtcagtcgcc agcgattctg atcagcggtc ttggtgcaat
    32941 ggctgccgtg ctgatgttgg tatttaaaga tccgattctt ggtctggtgg ccggtattca
    33001 gctttccgcg aacgatatgc tgaaactggg cgactggctg gagatgccga aatacggcgc
    33061 agatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtgcgtaact gggacaatac
    33121 cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact ggagcgggat
    33181 gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca ccagtattcg
    33241 ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg catttgttaa agccttattt
    33301 aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta cggagtcggt
    33361 attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga acgaatatct
    33421 gcgtaaccat ccgcggattc gtaaagatat gaccttaatg gtacgccaac tggctccagg
    33481 tgataacggt ttaccgctcg agatctacgc gtttactaac accgtggtgt ggctggaata
    33541 tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag agtttggtct
    33601 gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg catttaagca
    33661 gtaattaaaa aaaccgctct catcgaatgg atgagagcgg acatcgtttt gtcggatgta
    33721 gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa tacaatccac
    33781 accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag cagcaaaatc
    33841 atgccgagga aggcaatgca gagatagttt ccgaacggat agagcagcgc cttaaactgt
    33901 gtttcacgcc cctgacgtcg catcgctgca cgaaaacgca gatgcgccag acagatcata
    33961 atccagttca acagcagcgt tgctaccacc agcgccatca gcagaccaaa cgctttttgc
    34021 ggcagcagat agttgattaa caccaccagc gaagtgatcg ctccggaaag catcagcgag
    34081 ttaatcggca caccgcgacg gctgacgcga gtcaaaaact tcggcgcatt accctgcaca
    34141 gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac tgacagcgat
    34201 gctaccagaa tgacgaagtt cagcgcagaa gctaccacgt tgctgtcgag attatggaaa
    34261 atcatcacaa acgggctact gttggatttc acttccaccc acggatagag cgccagtaaa
    34321 accaccagtg aaccgatgta aaacagcagg atgcgataca ccacctgatt taccgctttt
    34381 ggaatgcttt tatccggatc gcgcgcttca gcgacagtaa tcccaatcag ctccagaccg
    34441 ccgaaggaga acataattac cgccagcgac aaaatcagcc cattccagcc ggtggcgaag
    34501 aaaccaccgt agcgccagag gttgtcgata ctggctttct cgccgccgtg accagaaaac
    34561 agcagccaca ggccaaagcc gatcataccg atgattgcca gcactttaat caacgcaaac
    34621 cagaactcgg tttcgccata taagcgcacg ttcaccaggt taacggcgtt gatgataata
    34681 aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg catatagatg
    34741 cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta gttccagcca
    34801 gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa tgaaccggat
    34861 accggctctt cgaccaccat ctcgccaagc tggcgcataa tcaggaaagc gatgatcccg
    34921 gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg accaatgcca
    34981 agaaacagac cagtaccaat tgcgccaccc agcgcaatca gttgaatatg acggttatgt
    35041 aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac ggttgacgcg
    35101 tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta acatttagtg
    35161 cccatcgtcg caagacacaa tccacacggt taaacggggt atcctgcttt tatttccgta
    35221 cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac agcgacagca
    35281 aaggtgcggt gatcatgccg ccaatcatcg gtgcggcaat ccggctcatc acctctgaac
    35341 cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc accgtcatcg
    35401 ctttcgggcg cacgcgcagt accgcgccgt gatacaacgc ctcatccagc ttctgctcac
    35461 tgaatgtttg cggattattc aacgacggct cggcctctat ggcgtgacgt aaatacatca
    35521 gcatcaccac gccaaattcg gcggcgaccc cggcaagggc aataaaaccg gtgcccgttg
    35581 ccacagaaag atgaaagccc atccaccaca gcaaccagat gccgcccacc agcgcaaacg
    35641 gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaatgccaga tacaacagca
    35701 cgaagatgat catcagtgtc atcggcacca tgagtttcag cttatggttg gcgcgctcca
    35761 gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc actttttcag
    35821 ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg gcatcgatat
    35881 aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg gtagagactt
    35941 taacgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg atcggtagct
    36001 ggcgcagtgc ctgcgggcta tcgcgccagc tttgcggata acgcagatta atgggataac
    36061 gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa gtcacaaaca
    36121 actgcacatc cgccaccgtc ataccgtaac gcgcggcttt ttcacggtta atctcaacgt
    36181 tgatatagcg cccaccttcc agacgctcgg ctagagcaga agccacgctt ggcaccgttc
    36241 gcgctacttc ttcaatttgc tcagccatcg cgtcgatatc cgccagcaca gtgccggaaa
    36301 ctttaatgcc gatggggctt ttaatgccgg ttgagagcat atcgatacgg ttacgaattg
    36361 gcggcaccca cagattcgcc agccccggca gccgcacggt gttatccagt tcctcaatga
    36421 ttttgtccat cgtcatgcca ggccgccact gatcctgcgg cttaagctgg atggtcgttt
    36481 caaccatctc gagcggagca gaatcggtgg cggtttccgc tttcccggtt ttgccaaata
    36541 cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc atactcgccg
    36601 cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg ccttcattga
    36661 tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc agcaccgaaa
    36721 gcgctgccac caacagcgtg gttttcggcc agtgcagtac tttcagcaat agaggatgat
    36781 aaacacgaat caaaaagcga ttgagcgggt tactgctttc cggcggaatt ttgccacgga
    36841 tccagtaccc catcaggata gggatcacta cgatcgccag cagcgccgcg cccgccatcg
    36901 cataggtttt agtgaacgcc aacgggccaa acagacgccc ttcctgccct tccagggtga
    36961 agatcgggat aaacgacaac gtgataatca gcagactgat aaatagcgcc ggacccactt
    37021 caacagatgc atcggtgatc acctgccagc gcgttttatt atccagcgtg gcgtcaggat
    37081 gctggtgctg ccactcttcc agccgtttat gtgcattttc gatcatgacg atagcggcat
    37141 cgaccatcgc cccgacggca atcgcaatgc cgcccaacga cataatattg gcattcagtc
    37201 cctggaagtg catgacaata aaagcaatac acaaccccag cggcaacgaa ataatcgcca
    37261 ccagcgccga gcgcacatgc cagagaaaca gcgcacagac taccgccacc acgataaact
    37321 cttccagcaa cttgccgctg aggttgtcga tggcgcggtc aatgagctgg ctgcgatcgt
    37381 atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc agtttgtcct
    37441 tcacggaggc gatcacttct cgggcgtttt tgccggagcg caggatcacc accccgcccg
    37501 ccacttcgcc ttcgccattc agttcggcaa tgccccggcg catctccggg ccaatctgga
    37561 ccttcgcaac atcacgcaga taaacgggca cgccattttc actggctttt aaaacgatgt
    37621 gattaaagtc gtcgagcgtt tgcagatagc cgctggcgcg caccatatat tccgcttccg
    37681 ccagttcgat cgacgaaccg cccgcttcct ggtttgaagc atccagcgcg ctttttactt
    37741 cggctagact gatgccatat tgcgccaggc gctggggatc gataaccacc tgatactctt
    37801 tcaccacgcc gcccaccgac gccacttccg caacgtcagg gatggttttt agctcatatt
    37861 tgagaaacca gtcctgcaat gagcgtaaat cggccagatc gtgcttaccg ctgcgatcca
    37921 ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggtcccagc tcggcgctga
    37981 ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc gaacgcgccc
    38041 agtacggatc ggtaccatct tcgaaaatga catacacata agagtctcca aactgcgaga
    38101 aaccgcgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc ggataagtca
    38161 cctgattttc aacgatttgc ggtgcctgac cgggatagct ggttttaata atcacctgca
    38221 catcggagag atccggcagc gcatccactg gcgtgttaat gatggtccag gtgccccaga
    38281 tgctcagaaa caacgcgccc atcagcacca aaaaacggtt cgccaccgag cgacgaataa
    38341 tccattcaat cattggttat tccctcaatg cgcattggta gcactttcag agcgcatccg
    38401 ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc tggaaaccac
    38461 cttttcacct tccgccagac cagagtgtaa cgcggtgacg ccttgcgatg cctggaaaac
    38521 ggcaacgcgt ttcggtacaa agcgtccgtc ggcatcaacg gtaatcaccc gctgttcgtt
    38581 gccggtatca atcagcgcct gtgacggaat gagcagcatc ggttcgctgg cggtgttgag
    38641 ttgcaaccag gcgttcatgc ccggttttag cgcctcgtcg gcgttgtcga cttccagacg
    38701 cagctgcagc gtgcgggtcg cggcatccac gccaggtagc agcgtccatt tgcggatggt
    38761 gagtgtttta tccggtcgcg ccggaacggt gagcgtaaac tgcgaggcat ctttcaccag
    38821 ccaggcgata gactccggga tcgcagcagt gacccacacc gggtccatac cctgaatttt
    38881 cgctaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg cggtgatcac
    38941 gccatcaatg ggtgctttga gcgtaaagcg agtctggatt ttttgcgtgg cgatcaggcg
    39001 gcgaatatcc gcctccggca ttcccgccag tcgcagccgc tcaagaatgc cttcagtctg
    39061 ggtcgccgta ccgccggttt cgcgcagcag taaatactca ctctgcgctt ccacccagtc
    39121 aggaatggtc aggtcgagaa gcggtgtgcc cttttgtact ttatcgccca cggtaagcgg
    39181 atacaccttg tcgataaacc cggcagcgcg agcctgcaca atggcatact gatactcgtt
    39241 gtaactgaca ttcgccggga aactctgggc aaaagtcagc ggtccgcgcg tgacggtagc
    39301 cgttttcacc cccagattct gcgtctgagt cgggtcaatg cgcacaccag acgcagaact
    39361 ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt tacctggttt
    39421 atcgaaccgc gtattggggt acatcgggtc gtaccagaat aagattttac gttctgcggt
    39481 cgacgttttt tctgcgggtg gttcagcctt tgcaaaccag gtaaaacctg ccgcagaaat
    39541 aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct cattaaacct
    39601 gggttactgg ctgactttaa tatcctgtaa taaagaaagg ttgccctgct ggacaaaatt
    39661 aaacgccact ttgtcgccgg ttttaatttc actcattttc gtctgcgggg tgatggtaaa
    39721 gcgcatagtc atctccggcc agttcacggc agcaatcgga tcgtgatgga tggtgatttt
    39781 tttgctttcc agatcaatac cctttaccac gccagtggcg ctaataacct gcggttgtac
    39841 ttcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa taacggtaaa
    39901 cagactgaac attgcgactt gcagtgcttt tttcatttat tctctcctgg agttaaaaat
    39961 tattgctgcc aaccgccccc taaggcggta tacaaagaaa tttcgttaac ctgacgggca
    40021 taattcaggt cgagtaaagt ttgtcgggtt gcaaataaag aacgctcggc atccagcact
    40081 tccagataac ttaccgcgcc gtgctgatat aatgcccgcg cccgttgcaa agtaatttgc
    40141 agcgacgcca gataacgctg ctgggcgctg atttgatcgt tcaggctttg acgtaatgcc
    40201 agcgcatctg ccacttcttt aaaggcgttc tggattttct gttcataatt cactaccgac
    40261 tgctgctggc gaatttcggc gatatccaga ttggcctggt tgcgtccggc attaaaaatg
    40321 gggatctcaa ttttaggaat aaaattccac atcccgctgc tagcgttaaa taatgaagac
    40381 agatcgctgc tggcggttga tattccgctg gtcaggctga tagacgggaa aaatgccgca
    40441 cgcgcagcac caatattagc attagccgcc attaacgcgt gttcagcttc cataatatcc
    40501 gggcgctgca ataagatttg cgacgacaag cccgccggta atttaacgct ttgcaggctg
    40561 ccgctgttta ctgtctgcgc ttgcggcagt ttgccgtagc ttcccaataa cagttgcaat
    40621 gcattattcg cctgcgccag ttccccctga cgtttagcga tgtcactgcg ggtactttct
    40681 atcaccccgc gagcctgttc cagcgccaga acattgctgc taccggtcaa cagttgtttt
    40741 tcgacaaacg catatgactg ctgataatta cgcagcgttt cttcggctat ttgcaattgc
    40801 gcatacgcca gttgctgatt gaaatagctt tgcgcgacat tagaaaccag cagaatatgc
    40861 accgcgcgct gagcttcctc agtggctaaa taattttgtc gctcggcttc gctcatgttc
    40921 tttaagcgac cgaaaaaatc gaggtcaaag ctggcgttaa ggccggtcga aaactcccgt
    40981 gtcgtggcgg tattcccttt aagattaccg ctccagctgc cgctgccctc gccattgagc
    41041 tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc gtgcctcctg cactttcagc
    41101 gtcgccatgc gcaaatcccg gttattcacc agcgcctcgc taatcagcgt cttcacctga
    41161 ttatcaacaa aaaaggtgcg ccagcccacg ttctggtagt tatctgctgc gttaaccaag
    41221 ccgttctggc tgagtgagaa ctgctgcggc acgggcattg ccgtacgctg ataatccggt
    41281 gccaatgaac aaccggttag cgcaagggcc acacaaaatg gcagaagttt acaaggagac
    41341 ataggctcat aatttctggt gattttatac cgccaacttt actcgccagg ctctgatttt
    41401 ccggtgacag gaaaatgaca aaattgtcat tttgccaata agcgattgcc atctgatccc
    41461 gctactctag aattgcccgg gcaacatgcg gaggaaaaat gaaactgttg attgtcgaag
    41521 atgaaaagaa aaccggagaa tacttgacca aagggttaac cgaagccggt tttgtggtcg
    41581 atttggccga caacgggctg aatggctacc atctggcgat gaccggtgat tatgacctga
    41641 taatcctcga tattatgctg ccggacgtga acggctggga tatcgtgcgc atgttgcgct
    41701 ccgccaataa agggatgccg attctgttgc ttaccgcgct tggcaccatt gaacatcgcg
    41761 tcaaggggct ggagttgggg gccgatgact atctggtgaa gccgttcgct tttgctgaac
    41821 tgctggcgcg ggtgcgcact ctcctgcggc gcggggcggc ggtgattatc gaaagtcagt
    41881 ttcaggttgc cgatctgatg gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc
    41941 gcatcactct gaccagtaaa gagtttactc tgctggagtt ttttctccgc catcagggcg
    42001 aagtgctgcc ccgctcgctt atcgcctcgc aggtgtggga catgaatttc gacagcgaca
    42061 ctaacgccat tgatgtggcg gtgaagcggc tacgcggcaa aatcgacaac gactttgagc
    42121 cgaagctgat tcagaccgtg cgcggcgtgg gctatatgct tgaggtgccg gatggtcagt
    42181 aagccatttc agcgcccgtt ttcgctggca acccgtctga ccttttttat cagcctggcc
    42241 accatcgcgg cgtttttcgc ctttgcatgg atcatgatcc actcagtgaa agtgcatttt
    42301 gccgagcagg atattaatga tttaaaagaa attagcgcca cccttgaacg ggtacttaat
    42361 catcctgacg agacgcaagc ccgacgctta atgacgctgg aagatatcgt cagtggttat
    42421 tccaacgtgt tgatttccct ggcagatagc cacggcaaaa cggtgtatca ctcccccggt
    42481 gcgccggata tccgcgagtt tacgcgtgac gccatacccg ataaagacgc tcagggtggc
    42541 gaggtgtatc tcctttccgg cccgacgatt atgatgccag gccacggtca cgggcatatg
    42601 gaacacagca actggcggat gattaacttg ccggttggcc cgttggtgga cggcaaaccg
    42661 atttatacgc tctacatcgc gctttcgatc gattttcatc ttcattacat aaatgatttg
    42721 atgaataaac ttattatgac cgcatctgtc atcagcatcc tgatcgtctt tatcgtgcta
    42781 ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatc
    42841 acctcgaaag atctcgacgt tcgccttgac ccgcagaccg agcccattga gctggaacag
    42901 ctggtactgt cgttcaacca tatgatcgag cgtattgagg atgtctttac ccgccagtcc
    42961 aatttctcag cggatatcgc tcacgaaatt cgcacgccga ttaccaatct catcacgcaa
    43021 acggaaatcg ccctcagcca gtctcgcagc cagaaggagc tggaagatgt gctctactct
    43081 aatctcgaag agctgacgcg aatggcgaaa atggtcagcg atatgctgtt tctcgctcag
    43141 gccgataaca accagctaat ccccgaaaag aaaatgctca acctggcgga tgaagtcggc
    43201 aaagtgttcg attttttcga ggcgttagcg gaagatcgcg gcgtggagtt gcgatttgtt
    43261 ggcgacaaat gtcaggttgc gggcgatccg ctgatgctgc gtcgggcgtt aagcaacctg
    43321 ctttctaatg ccctgcgtta tacgccaacc agagagacaa ttgtagtgcg ctgtcagacg
    43381 gtcgatcacc aggtgcaagt taccgtcgaa aaccccggta cgcccattgc gcccgagcac
    43441 ttaccgcgat tgtttgaccg tttctaccgc gttgaccctt cccgccagcg aaaaggcgaa
    43501 ggtagcggca ttgggctggc gatagtgaaa tcgattgttg tcgcgcataa aggcacggtt
    43561 gcagtaacgt cagatgcgcg ggggacaagg tttgtgatta tgctgccgga gagagagtga
    43621 ttattaagtt caaattcagg gcgtggtgca tcacctcctg gtattgttga tcaacaaatt
    43681 agagaaaaac ttattaaaca tatgaaatat cgaaggcttt tatttttcaa agtttcacga
    43741 gatgtttgta tatgaaactt agttaaatct gtgtttcttc caattgtaga tcacaatgcc
    43801 ctttctaaat tttcatcgtt ataaacaagc catctatcat ataaagcaat gaataattta
    43861 atcgcgatta atcatcatta attgttatat tattcactca atctatttgt caagtgaatt
    43921 tgataaattc ctaatttaaa atatccacta ttgagccagt atttaacaac gttaagatgt
    43981 gcaatgtgca gatcatgtta tcagggtgga gcggtagcat atatttctta tgcaatcatt
    44041 cattttaata atgttaattg gattatttat tgctgtgaat aaaaatatat tatgaatatt
    44101 ggtttaactt tgcgtttttg ttactcgttg tgatatgcgt acctgtcctg aataacgcca
    44161 agaaacaaga ggttgagctt gtgattattt ttttgactga acagacaccc gtttctgtaa
    44221 agtaatattt aaacaaaatt ctcacataac catagcattt tctgttcctt ccctaataac
    44281 gtctgaagtc gtcccgctta tcacattatc cgcagttgta cggtacggat cattattcat
    44341 tttactggag gttgctatgt caaccggatt acgtttcaca ctggaagtgg acggcctgcc
    44401 gccggacgct tttgcggtgg tttcctttca tctgaaccag tcactctctt cgctcttttc
    44461 cctcgatctc tccctggtca gccagcagtt tctctccctt gaatttgcgc aggtgctgga
    44521 caaaatggcc tacctgacga tatggcaggg cgatgaagta cagcgccggg tgaaaggcgt
    44581 ggtgacctgg tttgaactcg gggagaacga caaaaaccag atgctgtaca gcatgaaggt
    44641 gcacccgccg ctgtggcgtg ccggtctgcg ccagaacttc cgtatcttcc agaacgagga
    44701 catcaaaagc atcctcggca cgatgttgca ggaaaacggg gtgaccgaat ggagtccgct
    44761 gttcagcgag ccgcatcctt cccgtgagtt ttgtgtccag tacggtgaga ctgattacga
    44821 tttcctgtgc cggatggcgg cggaggaagg catcttcttt tatgaggagc atgcttacaa
    44881 aagtaccgac cagagcctgg tgctgtgcga cacagtccgc catctgcccg aatcttttga
    44941 aatcccatgg aacccgaaca cccgtaccga ggtgagcacc ctctgcatca gccagttccg
    45001 ctacagcgca caaatccgcc cttcttccgt ggtgaccaaa gactacacct ttaaacgccc
    45061 cggctgggcc ggacgttttg aacaggaagg ccagcaccag gactaccagc gcacgcagta
    45121 tgaagtgtat gactaccccg gacgtttcaa gggtgctcac gggcagaact ttgcccgctg
    45181 gcagatggac ggctggcgaa acaatgcaga aaccgcgcgg ggaatgagtc gctcgccgga
    45241 gatatggccg ggacgacgaa ttgtgctgac ggggcatccg caggcgaacc tgaaccggga
    45301 atggcaggtg gtggcaagtg aactgcacgg cgaacagcca caggcggtgc caggacggca
    45361 gggagcgggg acggcgctgg agaaccattt tgcggtgatc ccggcagaca gaacatggcg
    45421 accacagccg ttgctgaaac cgctggtcga cggcccgcag agcgctgtcg tgacaggacc
    45481 ggcaggcgag gaaatcttct gcgacgaaca tggtcgcgtg cgggtgaagt tcaactggga
    45541 ccgttataac ccggcagacc aagacagttc gtgctggatc cgtgtggcac aggcgtgggc
    45601 aggcaccggt tttggccacc tggcgatacc gcgtgtgggt caggaagtga ttgtagactt
    45661 cctcaacggc gatccggacc agccgatcat tatggggcgc acctaccacc aggaaaaccg
    45721 cccccccggc agcctgccgg gaacaaagac gcagatgacc atccgctcca aaacgtacat
    45781 gggcagcgga tttaatgagc tgaagtttga tgatgcgaca gggagagaac aggtctacat
    45841 ccacgcgcag aagaacatgg ataccgaagt gctcaacgac cgtaccacca ccgtaaaaca
    45901 cgatcaccgc gaaaccgtaa aaaatgacca gacggtcacg atccaggaag gtaaccgcct
    45961 tcttacggtg gaaaaaggcc acaagatcac cggagtactg aaagggtctt tatctgagga
    46021 tgtctttcag gacagaggca cgattgccgg ttcggtgcat gttgacgctg taaacaatgg
    46081 tggcgaaggc gacggtatac aggcttatac ggcgattaag gaaattttgc tggccgtgga
    46141 ggaaagcaaa attgcgctga cgccggatag cattcagcta caggtcgggg aatcgacggt
    46201 aatcaggctg tcgaaggatg gcatcaccat cgtgggcggt tctgttttca tcaactgatt
    46261 cactcagatt atcagggagt attttatgca gttcactttt aatgaaggcc acattcaact
    46321 gccgtctcaa tggcaggatc agtcaatgca ggtcctggtg tccacagata acagtggcat
    46381 caacctggtc attacccgcg aagccgttcc acagggaacc ctgacaccag agctgtacca
    46441 ggaaacgctg gcgctgtacc agggcaaact tgatgggtac accgaacatg cctgccggga
    46501 aatcactctg gcagaggccc cggcctggct gctggattat tcctggaacg gtccggaaga
    46561 tgaggggaat caggggcgga ttagccagat agccgtattc cagcggcgtg gagatacgct
    46621 gctgaccttc actttcagca cctccttatc cctgaagaac agtcaaaaga cgatgctgct
    46681 ggaggtgata aagagcttca ccccgttgcc accggagaat gacatccaaa aggaccagcc
    46741 gcgctgacat aaacgaggga taacacatga gtgaaggacc aggcgggcca cagggagcga
    46801 ccgcaggcgg tacgctggca atgcgaatgc tgtcacagca ggcgatggtc gccagccaga
    46861 tgaaacgggc agccaacgac aaagccattg cacagatgtt ggcatcaaaa aagtctggcc
    46921 cccccgccgc caggctgggc gatgaaattc agcacaagag ttttttgggg gcgctggcag
    46981 gggccgtact gggggcgata gtgaccatcg cagaaggttg cctgattatg gccgcctgtg
    47041 ccaccggccc ttatgcgctg gttctggtac ctgcgctgat gtatgccagc tataaggcga
    47101 gtgattatgt ggaggagaaa cagaaccagc ttgaatcatg gataaacagc ttttgtgaca
    47161 cggacggcgc catcaatacc ggttctgaaa acgtaaaaat taacggcgag ctggccgcac
    47221 gtgcagcggt cacccttccc cctcctcccc cacctggagc aatacctgaa gtcccacagg
    47281 gggaaccctc atggggtgat attgccactg acctgcttga atcggcagcg gaaaaagcag
    47341 taccactggc gaaggcctgg gggaacgctg ttatcaccct gacggacagc aatgccggtt
    47401 ttatggatcg cgtcagcgcc ggcgcatcgc ttctgtttcc cgccggtccg gtattaatgg
    47461 agtttgccac catggtgggc gggcgtggcg aaatcaaaaa agatgtggat ttcccggaag
    47521 ccggtgagga cacggcgctc tgcgacaagg agaacaaacc accgaggata gcccagggca
    47581 gcagcaacgt ctttatcaac aatcagcctg ccgcgcgcaa gggcgacaaa ctggagtgca
    47641 gtgcggcgat cgtgggaggt tcgccggacg tctttattgg cggtgagcag gtcacatatc
    47701 tggatatcca gccggagttc ccgccgtggc agagaatgat cctgggggga ataacgatag
    47761 ccagctatct tctgccgcca gcgggactac tgggaaaact gaagaatctg gcgagactcg
    47821 gcaaactggg aaactgctgg ggaaaagcgg gaagctgctg ggcgcaaagc tcggcgcgtt
    47881 gctggggaaa acaggtaagt cgttaaaaag tattgccaat aaagtcatca gatgggtaac
    47941 agatcctgtc gatccggtaa ccggcgcata ctgcgacgaa cgtaccgact tcaccctggg
    48001 ccagaccctc cccctctcct tcacccgttt ccacagttct gtactgccgc tgcatggcct
    48061 gaccggcgtg ggctggagcg actcctggag cgaatacgcc tgggtgcgtg aacagggaaa
    48121 ccgggtggat atcatcagcc agggagccac gctgagattt gccttcgacg gtgacagtga
    48181 tacggcggtt aacccgtatc acgcccagta cattctgcgc cgccgcgatg attatctgga
    48241 gctgttcgac agggatgcac tgagcagccg cttcttttat gacgcctttc cgggaatgcg
    48301 tctgcgccac ccggtgactg acgataccag cgatgaccgc ctggcacaca gccccaatga
    48361 ccggatgtac atgctgggcg ggatgagcga caccgccagc aaccgcatca cgtttgagcg
    48421 cgacagccag taccggatca cgggtgtcag tcacaccgac gggatccggc ttaaactgac
    48481 gtaccacgcc agcggctacc tgaaagccat tcaccgcacg gataacgaca tacagacgct
    48541 ggcgacctac gaacaggatg cgcgggggcg gctgacagaa gcggatgcgc ggctggacta
    48601 ccacctgttt tatgagtacg acgctgcgga ccggatcatc cgctggtccg ataacgacca
    48661 gacgtggagc cgtttcacct acgatgaaca gggccggtgc gtgaatgtca ccggggcgga
    48721 gggctattac aacgccacgc tggactatgg tgacggctgc accaccgtga cggacggcaa
    48781 gggcattcac cgttattact atgatcctga cggcaatatt ctgcgggaag aagcgccgga
    48841 cggcagcacc accatgtatg aatgggatga attccatcac ctgctggccc gccactcccc
    48901 tgccgggcgg gtggagaaat ttgaatacaa cgccgcacac ggtcagttaa gccgttacac
    48961 ggcggcagac ggcgcggagt ggcagtaccg ctatgatgag cgcggcctgc tcagcaacat
    49021 caccgaccct gccgggcaga cgtggacaca gcagtgtgat gaacgcggcc tgccggtaag
    49081 tctggtgtcg ccacagggcg aagagacccg gctggcatac accgctcagg ggctgctgtc
    49141 gggaatattc cgccaggatg aacggcgtct gggcatagag tacgaccacc acaaccggcc
    49201 ggaaacactc accgacgtga tgggccgtga acaccacacc gaatacagcg gtcacgacct
    49261 gccagtgaag atgcgcggcc ccggcggtca gtcagtgcgg ttacagtggc agcagcacca
    49321 taaactgagc ggcattgaac gtgctggaac cggcgcggaa ggatttcgct atgaccgcca
    49381 cggcaacctg ctggcctgga cggacggcaa cggtgttgtc tggacgatgg aatacggtcc
    49441 gttcgatttt ccggtggcgc gaacggacgg tgaaggccac cgctggcagt accgctacga
    49501 taaagacacg ctgcaactga cagaagtcat caacccgcag ggtgagtcat atctttatgt
    49561 tctggataac tgcggccggg tgacggaaga gcgtgactgg ggcggcgtgg tctggcgtta
    49621 ccgctatgac gccgatggcc tctgtaccgc cagggttaac ggcctggagg aaaccatcct
    49681 ctacagccgg gacgccgcag gccgcctggc agaaatcatc tcgccggaag gcaaaacgca
    49741 gtatgcctat gacaaatccg gcaggctgac gggtatcttc agcccggacg gcacatcaca
    49801 gcgcaccggc tatgacgaac gcgggcgggt gaatgtcacc actcagggcc gacgggccat
    49861 tgaatacaac taccccgatg aacacaccgt catccgctgt atcctgccac cggaagatga
    49921 acgcgacaga caccccggtg aatccctgct gaaaaccacg taccgctaca acgccgccgg
    49981 agaactgaca gaggtcattc tgccggggga tgagacgctg acgttcagcc gtgatgaggc
    50041 gggacgtgaa gtgctccggc acagtaaccg gggttttgcc tgtgaacagg gctggaatgc
    50101 agccggtcag cctgtcagtc agcgcgccgg attttttccg gcagaagcca catgggacgg
    50161 gctggttccc tcactggtac gggagtaccg ttacgacagc gcgggtaacg tgtcaggcgt
    50221 caccagccgg gaagattacg gacgggaaac acggcgggag tatcggctgg accggaacgg
    50281 ccaggtcacg gcggtgacag cctcaggcac cgggctgggc tatggcgaag gcgatgagtc
    50341 ctatggctat gacagctgcg gctacctgaa ggcgcagtct gcgggcaggc accggataag
    50401 tgaagagact gaccagtatg ccggaggcca ccggctgaaa caggccggaa acacgcagta
    50461 tgactatgac gccgcaggcc ggatggtcag ccgcaccaga caccgtgacg gctaccgacc
    50521 ggaaacagag cggttccggt gggacagccg ggaccagctg accgggtatt gcagcgcaca
    50581 gggtgagctg tgggaatacc gccacgacgc cagcggcaga cgaacagaga aacgctgcga
    50641 ccggaagaaa atccgcttca cgtatctgtg ggacggcgac agtattgcgg aaatccggga
    50701 ataccgcgat gataaactgt acagcgtaag gcacctggtg tttaacggct ttgagctgat
    50761 aagccagcag ttcagccggg tacggcaggc gcatccgtcg gtggccccgc agtgggtgac
    50821 gcggacgaat catgcggtga gcgacctgac gggccgcccg ctgatgctct ttaacagtga
    50881 aggaaagacg gtctggcgac cggggcagac cagcctgtgg gggctggcac tcagcctgcc
    50941 cgcagacacc ggctacccgg acccgcgcgg ggaactggac ccggaagccg accccggcct
    51001 gctgtatgca ggacagtggc aggatgcgga atccgggctg tgctataatc ggttccggta
    51061 ttacgagccg gaaaccggaa tgtacctggt gagtgatccg ctggggttgc agggcgggga
    51121 gcagacttac cggtatgtgc cgaatccttg tgggtgggtt gatccgctgg gattggctgc
    51181 aagttctaaa atcagcagtt tgatggacta tattggcgat ggtcgtcgtg ttagtgggca
    51241 tacgggtttc ctggatgggg ttcgtttatc acgtagtcaa ataa
//
LOCUS       DAQSWQ010000020        46701 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.020, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000020 DAQSWQ010000000
VERSION     DAQSWQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.020"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="R8G18_001188"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="R8G18_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HEE9794949.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="R8G18_001189"
     CDS             complement(280..873)
                     /locus_tag="R8G18_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEE9794950.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="R8G18_001190"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="R8G18_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEE9794951.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="R8G18_001191"
     CDS             1788..2183
                     /locus_tag="R8G18_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEE9794952.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="R8G18_001192"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="R8G18_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HEE9794953.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="R8G18_001193"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="R8G18_001193"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEE9794954.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="R8G18_001194"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="R8G18_001194"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEE9794955.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="R8G18_001195"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="R8G18_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HEE9794956.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="R8G18_001196"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="R8G18_001196"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEE9794957.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="R8G18_001197"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="R8G18_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HEE9794958.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="R8G18_001198"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="R8G18_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HEE9794959.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="R8G18_001199"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="R8G18_001199"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HEE9794960.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="R8G18_001200"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="R8G18_001200"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEE9794961.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="R8G18_001201"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="R8G18_001201"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEE9794962.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="R8G18_001202"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="R8G18_001202"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HEE9794963.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="R8G18_001203"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="R8G18_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HEE9794964.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="R8G18_001204"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="R8G18_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEE9794965.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="R8G18_001205"
     CDS             17158..17709
                     /locus_tag="R8G18_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEE9794966.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="R8G18_001206"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="R8G18_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEE9794967.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="R8G18_001207"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="R8G18_001207"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEE9794968.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="R8G18_001208"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="R8G18_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HEE9794969.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="R8G18_001209"
     CDS             complement(21354..22376)
                     /locus_tag="R8G18_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9794970.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="R8G18_001210"
     CDS             complement(22390..23892)
                     /locus_tag="R8G18_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEE9794971.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="R8G18_001211"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="R8G18_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HEE9794972.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="R8G18_001212"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="R8G18_001212"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEE9794973.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="R8G18_001213"
     CDS             complement(26207..26548)
                     /locus_tag="R8G18_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEE9794974.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="R8G18_001214"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="R8G18_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HEE9794975.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="R8G18_001215"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="R8G18_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HEE9794976.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="R8G18_001216"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="R8G18_001216"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEE9794977.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="R8G18_001217"
     CDS             33227..34570
                     /locus_tag="R8G18_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEE9794978.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34667..34873)
                     /locus_tag="R8G18_001218"
     CDS             complement(34667..34873)
                     /locus_tag="R8G18_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HEE9794979.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35198..35752
                     /locus_tag="R8G18_001219"
     CDS             35198..35752
                     /locus_tag="R8G18_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HEE9794980.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35842..36753)
                     /locus_tag="R8G18_001220"
     CDS             complement(35842..36753)
                     /locus_tag="R8G18_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9794981.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPV"
     gene            complement(36965..37705)
                     /gene="cysQ"
                     /locus_tag="R8G18_001221"
     CDS             complement(36965..37705)
                     /gene="cysQ"
                     /locus_tag="R8G18_001221"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEE9794982.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37895..39838
                     /gene="cpdB"
                     /locus_tag="R8G18_001222"
     CDS             37895..39838
                     /gene="cpdB"
                     /locus_tag="R8G18_001222"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HEE9794983.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39956..40336)
                     /locus_tag="R8G18_001223"
     CDS             complement(39956..40336)
                     /locus_tag="R8G18_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEE9794984.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40425..41285
                     /gene="qorB"
                     /locus_tag="R8G18_001224"
     CDS             40425..41285
                     /gene="qorB"
                     /locus_tag="R8G18_001224"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEE9794985.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41393..42358
                     /locus_tag="R8G18_001225"
     CDS             41393..42358
                     /locus_tag="R8G18_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEE9794986.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42466..43128
                     /gene="ytfE"
                     /locus_tag="R8G18_001226"
     CDS             42466..43128
                     /gene="ytfE"
                     /locus_tag="R8G18_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HEE9794987.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43173..44585)
                     /gene="cycA"
                     /locus_tag="R8G18_001227"
                     /gene_synonym="dagA"
     CDS             complement(43173..44585)
                     /gene="cycA"
                     /locus_tag="R8G18_001227"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HEE9794988.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44894..45514)
                     /gene="fklB"
                     /locus_tag="R8G18_001228"
     CDS             complement(44894..45514)
                     /gene="fklB"
                     /locus_tag="R8G18_001228"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEE9794989.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45733..46371
                     /gene="ytfB"
                     /locus_tag="R8G18_001229"
     CDS             45733..46371
                     /gene="ytfB"
                     /locus_tag="R8G18_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HEE9794990.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46355..>46701)
                     /locus_tag="R8G18_001230"
     CDS             complement(46355..>46701)
                     /locus_tag="R8G18_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEE9794991.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11459 a        12420 c        11634 g        11188 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcacttttta ctgcatagca ctttggttaa gccatctctg
    34681 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    34741 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    34801 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    34861 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    34921 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    34981 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    35041 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    35101 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    35161 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    35221 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg
    35281 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    35341 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    35401 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    35461 gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg cgattccggg
    35521 ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    35581 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    35641 cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg gggcgctcac
    35701 tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaaca
    35761 acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    35821 ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt taaacgcctc
    35881 aatggtcgac ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    35941 attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc aacctgcatt
    36001 aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag aggaaccatt
    36061 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    36121 ggcaaaatta tcatcaatta ttttttcgtg aatataccat ccatcgcatt ctttaattcc
    36181 acctcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta tatcctccaa
    36241 taaggcataa cctaaagaca cgtcaccaat cgcctggtga atataacctt taatcctttt
    36301 cggtatgtca gactgttttt tctgcagaat atcacaggca tacaatcttc tgttttgttc
    36361 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    36421 aatttcttcg tattcaaatc cactacctcg caatgtcatt gtatattcgg caccgttatc
    36481 tttaaggctg aaactgataa ggaacttatc catggcgtta acagttccat tttttgatga
    36541 aagtacctga taactatttt tatcaatctg agttgcgcaa cgaaagaact ccctcaaagt
    36601 taacaggcta tcataattcg aaatccaatc ttttttctta aatgcatgct ggatattgtt
    36661 aaaaaaattg tttgaatgtg tttttgtata cgatgacttt acaaataact ccaccgcacc
    36721 aataaaatta ttgtgtctga tcctgggtaa catatgacaa ccttatttta tttggttatt
    36781 ttatgtcgcc attaaatatc tcattagaga agtaaactat caaaaaacgc tcattattaa
    36841 aatgtaactt attagcaatt gaatttttgc actttaaata caatctcttt ttatttataa
    36901 ctttttaggt tgggagaaat caggatggca gaatcaggaa atacactgtt tctgccatct
    36961 gaatttagta aatagacact ctgaaccccg gattcaggaa cgactcgcgc ggagtgtaat
    37021 ccagcggttt accctgccag tcgtgaacgt gcgctccggc agctgcagcc acagcatgtc
    37081 cagcggcggt gtcccaaata ttcgttggtc cgaagcgcgg gtacagctgc gcctgtcctt
    37141 ccgccaccag gcagaatttc agcgaagagc cgatggacgt ggtctgatgt tcgccaagct
    37201 gttgcagata ctctttcagc tccgcatctg catgggaacg gctgataacc accagcggcg
    37261 ggcgcgcatc gcggacctga atctgcttgc gcacaccgca ctcttctttc caggccttgc
    37321 cttctgccgc gctgtacatt acgttcatta ccggcgcata caccacacct aaaatcggtt
    37381 tgccatggtc aatgagcgca atgttaacgg tgaattcgcc attacgttta ataaactctt
    37441 tagtaccatc cagcgggtct accagccagt aacgctgcca gtgctgacgg acttcccaac
    37501 cgggaggatc ttcttcagaa aggaccggaa tatccggtgt cagcgtacgt aaaccgtcca
    37561 tgataacggt gtgtgcggca atatccgctg ccgttaccgg agaattgtcc gctttgctga
    37621 cgacgtccat cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct gcattccgtg
    37681 caagctggca tacttgatct aacatttctc cacctcgtct ctgtgagcgg tgttaactta
    37741 ttgttttact tataccctat cgttaatgaa tgcgccaact gtgatagtgt catcattttc
    37801 aaagcgtaaa attgtggcat tcttcactgt tctataagta agacgtttat tctccctttt
    37861 ctttcgtatt cccgatgata aaaggatgtc cctgatgatt aagtttagcg caacgctcct
    37921 ggccacgctg attgccgcca gtgtgaatgc agcgacggtc gatctgcgta tcatggaaac
    37981 cactgatctg catagcaaca tgatggattt cgattattac aaagacaccg ccacggaaaa
    38041 attcggactg gtacgtacgg caagcctgat taacgatgcc cgcaatgaag tgaaaaacag
    38101 cgtactggtc gataacggcg atttgattca ggggagtccg ctggcagatt acatgtcggc
    38161 gaaaggatta aaaacaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta
    38221 tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc
    38281 gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca
    38341 gccaatgttt acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa
    38401 acagacgctg aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa
    38461 agctaattta tccgggaaag tgacggtgaa tgatattacc gaaaccgtgc gcaaatacgt
    38521 gcctgaaatg cgcgagaaag gtgccgatgt cgttgtcgtt ctggcacatt ccgggctgtc
    38581 tgccgatccg tataaagtga tggcggaaaa ctcagtttat tacctcagtg aaattccggg
    38641 cgttaacgcc attatgtttg gtcatgctca cgccgttttc ccgggtaaag attttgctga
    38701 tatcgaaggg gctgatatca ccaaaggcac gctgaatggt gttccggcgg taatgccggg
    38761 catgtggggc gatcatcttg gggtggtcga cttacaactc agtaatgaca gcggtaaatg
    38821 gcaggtgacg caggcgaaag cggaagctcg gccgatttac gacatcgcca ataaaaaatc
    38881 cctcgcggcg gaagacagca agctggtaga aacactcaaa gccgatcacg atgccacacg
    38941 ccagttcgtc agcaagccaa tcggtaaatc tgccgacaat atgtatagct atctggcgct
    39001 ggtgcaggac gatccgaccg tgcaagtcgt gaacaacgcg caaaaagcgt atgtcgaaca
    39061 ttacattcag ggcgatccgg atctggcaaa actgccggtg ctttcagctg ccgcaccgtt
    39121 taaagttggt ggtcgcaaaa atgacccggc aagctatgtg gaggtggaaa aaggccagtt
    39181 gaccttccgt aatgccgccg atctttatct ctaccccaat acgctgattg tagtgaaagc
    39241 cagcggtaaa gaggtgaaag agtggctgga gtgctccgcc ggacagttta accagattga
    39301 tcctaacagc acgaaaccac agtcactcat caactgggat ggtttccgca cttataactt
    39361 tgatgttatt gatggtgtga attatcagat tgatgttacc cagcccgccc gttatgacgg
    39421 cgagtgccag atgattaata ccaatgcgga aaggattaag aacctgacct ttaatggcaa
    39481 gccgattgat ccgaacgcca tgttcctcgt tgccaccaat aactatcgcg cttacggcgg
    39541 caaatttgcc ggtacgggcg acagccatat cgcttttgct tcaccggatg agaaccgctc
    39601 ggtgctggca gcgtggattg ctgatgagtc gaaacgtgcg ggggaaattc acccggcggc
    39661 agataacaac tggcgtttag caccgatagc tgccgataag aaactggata tccgtttcga
    39721 aacttccccg tcaaataaag ccgcagcgtt tattaaagag aaagggcagt atccgatgaa
    39781 taaagtcgcg accgatgata tcgggtttgc gatttatcag gtggatttga gtaagtaaaa
    39841 cacttctttt tcggcctata aatcatcaat cgcatccggc atttattggc ggatgcgatg
    39901 ctggcgcatc ttatccgccc tacaagtcat acaccgtagg ccgcgcatcc ggcagttatg
    39961 ccgcacgttc atcccgcacc gccaacacct cgggcaaatt caactcaatc cagtccgcca
    40021 gtgcagcaac cttttcgctc acctgctcgc ccagcggcgt gaggctgtat tcaacatgcg
    40081 gtggcaccac cggatacgcg atacggttaa gaaaaccatc ctgttctaac gcctgtaacg
    40141 actgcgcaag catcttttcg ctcaccccgc cgattttgcg ccgcaggtcg ctaaagcgat
    40201 gagtaccttc gcgtagcgcc accagaatca acacccccca acggctggtg acgtgtttca
    40261 acacctcgcg cgacgggcac tgttccgcaa agagattacc ctctttcagt tgttgcgaca
    40321 ggctaacctg actcatttca tacttacctt tttgtacgta cttactaaaa gtaagtttag
    40381 gtgttagcgt atttaaacac aagacaaaac gatggagact tcccatgatc gctattactg
    40441 gtgccactgg ccaacttggt cactatatta ttgaatcctt gatgaaaacg gttcctgcca
    40501 gccaaatagt ggctatcgtt cgtaatccgg caaaagccca ggctctagca gcacaaggca
    40561 ttaccgtgcg tcaggctgac tacggcgatg aagccgcact gacatctgca cttcagggag
    40621 tggaaaaact actgctgatc tcttccagcg aagtgggtca acgtgccccg cagcatcgta
    40681 atgttattaa tgccgcaaag gcggctggcg tgaaatttat cgcttatacc agcctgctac
    40741 atgcagatac ctccccgctc ggcctcgccg atgagcacat tgagacggag aaaatgctgg
    40801 ctgattctgg catcgtttac accctgctgc gcaacggctg gtacagcgaa aactacctcg
    40861 ccagcgcccc ggcagcactg gaacacggcg tatttatcgg tgcggcgggc gatggcaaaa
    40921 tcgcctcagc aacgcgggca gattatgcgg cagctgcggc acgcgtgatt agcgaagccg
    40981 gtcacgaagg caaggtttac gaactggcgg gcgatagtgc ctggacgttg acacagttag
    41041 cggcagagct gaccaaacag agcggcaaac aggttaccta tcaaaatctg agcgaagccg
    41101 atttcgccgc ggcactgaaa agcgtcggac tgcccgacgg actggcggat atgctggcgg
    41161 attctgacgt tggcgcatcg aaaggtggtc tgtttgataa cagcaaaacg cttagcaaat
    41221 tgattggccg cccaacgaca acgttagccg aaagcgtaag ccatcttttt aatgttaata
    41281 actagttaat taaagtggca tcctcccgca tcctctctga taatgacggg atgccgggag
    41341 caatcatgtc tgcttcctga actttcttct gacagaccaa tggatgccag taatgattag
    41401 cggcgtgctg tacgccctgt tagcagggtt gatgtggggg cttatttttg tcgggccgtt
    41461 gatcgtaccg gaatacccgg cgatgttgca gtcgatgggg cgttatctgg cgttagggtt
    41521 aattgcgctg cccattgcct ggctgggacg cgtgcgtctg cgtcagttgg cgcgtcgcga
    41581 ctggcttacc gccttgatgc tcaccatgat gggcaacctt atctattact tctgccttgc
    41641 cagtgccatt caacgtactg gcgcgcctgt ttccacgatg attatcggca ccctgccggt
    41701 ggtgattccc gtttttgcca atctgcttta tagccagcgc gacggcaaac tcgcatgggg
    41761 aaaactcgcc ccggcactgg tttgtattgg catcggcctg gcgtgtgtga atattgctga
    41821 gttaaaccac ggactccccg attttgactg ggcacgttat acctctggca tcgtgctggc
    41881 gttagtttcc gtggtctgct gggcgtggta tgccctgcgc aacgcccgct ggctgcggga
    41941 aaatcccgac aaacatccga tgatgtgggc aacggcgcag gcgctggtca cactgccggt
    42001 ttctctcatc ggctatctcg tcgcctgtta ctggctgaat atacaaacgc cggacttctc
    42061 cttacctttt ggcccccgtc cgctggtgtt tattagtctg atggttgcga tagccgtgct
    42121 ttgctcatgg gttggcgcac tctgctggaa cgtcgccagc cagcgattac cgacagtgat
    42181 tctcgggccg ctgattgttt tcgaaacgct ggcaggtttg ctgtacacct ttttactccg
    42241 ccagcaaatg ccgccgctaa tgacgctgag cggtatcgcg ctgttagtga ttggcgtggt
    42301 cattgcggtc agagcaaaac cagaaaaacc tttaactgaa tctgtctcag aaagttgaca
    42361 cgctggcagt gagttaaata agcctctgct acgtaagggt tatagctttt gccttaaaga
    42421 tgcatttaaa atacatctta tattattaag aatgaggtat cagctatggc ttatcgcgac
    42481 caacctttag gtgaactggc gctctctatt cctcgcgctt cagctctgtt tcgtaaatat
    42541 gatatggatt actgctgtgg cggtaagcaa acgctggcgc gcgcggcggc acgtaaagaa
    42601 ctggatgttg aggtcattga agctgaactg gcaaagcttg ctgaacaacc gattgagaaa
    42661 gactggcgta gcgccccgct ggcagaaatc attgaccata tcatcgtgcg ctaccacgat
    42721 cgtcaccgcg agcaactgcc ggagctgatc ctgcaagcga ctaaagtcga gcgcgttcac
    42781 gccgacaaac cgagcgtgcc aaaagggctg acaaaatacc tgactatgct gcatgaagag
    42841 ctatccagcc acatgatgaa agaagagcag atcctcttcc cgatgatcaa acaaggcatg
    42901 ggcagccagg caatgggacc aatcagcgtg atggaaagcg agcacgatga agcgggcgaa
    42961 ctgctggaag tgattaaaca caccaccaat aacgtcacac cgccgccgga agcctgcacc
    43021 acctggaaag cgatgtataa cggtattaat gaactgattg atgacctgat ggatcacatc
    43081 agcctggaaa acaatgtact gttcccacgc gcgctggcgg gtgagtaatc taaaagctgg
    43141 atggcattgc gccatccagc atgataatgc ggttatttcc gcagttcagc agcccgcttc
    43201 ttaccaataa acaaccagcc cagccccagt gcgataaacc acagcggggt gaccaacagc
    43261 gcctggcgag tgtcatcttc cagtgtcagc aacacgacca cgaacacaaa gaacgccata
    43321 catacccagc acatcagctt gccgagcggc atcttgtaga tcgacttctc atgcagatga
    43381 ggacgctgtt tgcggtaaac caggtacgag caaaggataa ttgtccagac gaacataaac
    43441 agaatcgcgg aaacggttgt aatcatcgtg aacgcgccaa tcacgctagg attcacatac
    43501 aacatcacca cgccgccgag cagacagata cacgagaagg tcaacccttt cgcgggtact
    43561 gcgcgcttag aaagtttagc gaacgctttc ggtgccacac cttcctgcgc cagaccaaac
    43621 agcatacggc tggtagagaa gacgccgctg ttagcggaag acgccgcaga ggtcagcacc
    43681 acaaagttga tcacgctggc agccgcaggc aaacctacca acacaaacag ttcaacaaac
    43741 gggctcttct ccgggactac cgaactccac ggcgtcacgg acataatcac aatcagcgcg
    43801 aagacgtaga acataatgat acggatcgga atggagttaa tcgcgcgtgg cagtgatttc
    43861 tccggatctt tggtttccgc agcggttgta cctaccagct caatccctac gaaagcgaaa
    43921 accgctatct ggaatccggc aaagaagcca cttaaacctt tcgggaacca gccgccatca
    43981 ttccacaaat gcgcgaatga cgcttccaca ccagtcggtg actgaaagtg catcgccacc
    44041 ataaccaggc cgacgacaat cagcgacaca atggcgacga ttttgatcat cgcaaaccag
    44101 aactccatct caccgaacat tttcacggtg gcgagattga gcgtcagcag cagcactatc
    44161 accgccagcg aggcgaccca gtcggagaga tcggggaacc agaactgagc ataagcagtg
    44221 atcgccacca cgtctgccat accggttaca acccagcaga accagtaagt ccagccggtg
    44281 aaatatcctg cccacggccc gagtaaatcg gaagcgaagt cactaaaaga tttgtattcc
    44341 agattcgaaa gcagcaattc ccccattgcc cgcatcacga aaaagagcat aaaaccgatg
    44401 atcatataaa cgaaaatgat cgacggcccg gcaaggctaa tcgttttgcc ggaccccata
    44461 aacaaccccg taccaatggc accgccaatg gcaataagct gaatatgtcg gtttgtgaga
    44521 ttgcgccgta gcgactgttc agccggagcc tgatcatcgg caacgacttt tacctgatct
    44581 accatgtttt tttcttcctg tacctgtctg tgttgttcag gctctacggc ctttagtcag
    44641 tctatgatac gacgaatctg tatcgggact catcgatatt aggtaacaat acgcgggatg
    44701 aatactaaga tttagatcta atgttaattt tatgtttaaa gtgagtgtca tatcacggtg
    44761 ataacgcgaa acagctcaca aaaatacacg cttacggggt ctttgcaaca caaaatagcg
    44821 ccgtttggca aactgaaggg tttattgctg aatgcctgct cccctctggt tcgaggggaa
    44881 cagaatgcgc tgcttagagg atttccagca gttcgacttc aaacaccagg gtgctgaacg
    44941 gagggatgga tgcgcctgcg ccgcgctcgc catatgccag ttcctgcggg atagtcagtt
    45001 cccatttaga acctaccggc atcagagtca gtgcttcaat ccagccaggg atcacgccat
    45061 taaccgggaa ttcagcgggt tcaccacgag caacggagct gtcaaacacg gtgccgtcga
    45121 tcagtttacc ggtgtaatga acacgaacgc ggtcggtacg tgccggaatt gcgccttcac
    45181 cctggttgat cacgcggaat tgcaggccag attcggtgct attcacacct tcttttttgg
    45241 cgttttcttc caggtatttc acaccttcag cagccatcgc ctggaaacgc tgacgacgaa
    45301 cggcatcggc gcgttcgtgg atttcacgca gcgcgcgatg caccacatca accggcacag
    45361 ccggatgttt gccttccagc gcatcggcaa tacctgcaac cagtgcttct ggcagcagcc
    45421 cttccaggcc agattcactc agttgttgcc ctacctgcaa accaatgccg tagcttgctt
    45481 gcgcttcgat ggtgtcaaaa gttggggtgg tcatcgtatt tcctttctta gggtataaaa
    45541 gtagcgggca gcatatcagc cacacttctt tgggtaaaac tttgtcgcag gaatgatgac
    45601 aaatcccggg gaaaaagaaa caatagcaat atcccgtatg aatataataa aggcgggttt
    45661 accacggaca tcatagcgtt agcttattca taggctatga ttgaggaaca agacgcggag
    45721 caggaggaaa ccatgcccgg gcgctttgaa ctaaaaccaa ccctggagaa agtctggcac
    45781 gcgccggaca attttcgctt catggacccg ctgccgccga tgcatcgtcg ggggatcatt
    45841 attgccgcca ttgtattggt ggtcggattt ctgcttccat ctgatgatac gcccaacgcg
    45901 ccggtggtga cgcgtgaagc gcagttagat attcagtcgc aatcacaacc gccgacagaa
    45961 gaacagctgc gggcgcaatt agtcacacca caaaatgatc ctgatcaggt ggctccggtc
    46021 gctccagaac ctatacagga aggccagcca gaagaacagc cgcaaactac ccaaacgcaa
    46081 ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt
    46141 aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg
    46201 gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa
    46261 atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa
    46321 caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt
    46381 caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct
    46441 ggggtgaaac catactgatg tgataaacca ggccaagcgt atgctggttg atcaaataat
    46501 atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatatct ggcttaaaaa
    46561 tattgtgttt cttcccttca tccaataagg attccattaa ctgcaagtgc gcatggttaa
    46621 tttctaagag acgcagtgat tttgcataat gcacaccttt actttgattt tcactatgca
    46681 caatttttaa aaaccaaggg t
//
LOCUS       DAQSWQ010000021        46161 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.021, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000021 DAQSWQ010000000
VERSION     DAQSWQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.021"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..715)
                     /locus_tag="R8G18_001231"
     CDS             complement(<1..715)
                     /locus_tag="R8G18_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEE9794992.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSS"
     gene            complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="R8G18_001232"
     CDS             complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="R8G18_001232"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEE9794993.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="R8G18_001233"
     CDS             complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="R8G18_001233"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HEE9794994.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3649..4704)
                     /locus_tag="R8G18_001234"
     CDS             complement(3649..4704)
                     /locus_tag="R8G18_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEE9794995.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4714..5502)
                     /locus_tag="R8G18_001235"
     CDS             complement(4714..5502)
                     /locus_tag="R8G18_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEE9794996.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5520..6572)
                     /locus_tag="R8G18_001236"
     CDS             complement(5520..6572)
                     /locus_tag="R8G18_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEE9794997.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6603..7445)
                     /locus_tag="R8G18_001237"
     CDS             complement(6603..7445)
                     /locus_tag="R8G18_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEE9794998.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7432..8313)
                     /locus_tag="R8G18_001238"
     CDS             complement(7432..8313)
                     /locus_tag="R8G18_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEE9794999.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8334..9644)
                     /locus_tag="R8G18_001239"
     CDS             complement(8334..9644)
                     /locus_tag="R8G18_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEE9795000.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9658..11189)
                     /locus_tag="R8G18_001240"
                     /pseudo
     CDS             complement(9658..11189)
                     /locus_tag="R8G18_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11401..11715)
                     /gene="pspE"
                     /locus_tag="R8G18_001241"
     CDS             complement(11401..11715)
                     /gene="pspE"
                     /locus_tag="R8G18_001241"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HEE9795001.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11790..12011)
                     /gene="pspD"
                     /locus_tag="R8G18_001242"
     CDS             complement(11790..12011)
                     /gene="pspD"
                     /locus_tag="R8G18_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HEE9795002.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12020..12379)
                     /gene="pspC"
                     /locus_tag="R8G18_001243"
     CDS             complement(12020..12379)
                     /gene="pspC"
                     /locus_tag="R8G18_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HEE9795003.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12379..12603)
                     /gene="pspB"
                     /locus_tag="R8G18_001244"
     CDS             complement(12379..12603)
                     /gene="pspB"
                     /locus_tag="R8G18_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HEE9795004.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12657..13325)
                     /gene="pspA"
                     /locus_tag="R8G18_001245"
     CDS             complement(12657..13325)
                     /gene="pspA"
                     /locus_tag="R8G18_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HEE9795005.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13492..14469
                     /gene="pspF"
                     /locus_tag="R8G18_001246"
     CDS             13492..14469
                     /gene="pspF"
                     /locus_tag="R8G18_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HEE9795006.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14587..15852)
                     /gene="puuE"
                     /locus_tag="R8G18_001247"
                     /pseudo
     CDS             complement(14587..15852)
                     /gene="puuE"
                     /locus_tag="R8G18_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15890..17170)
                     /gene="puuB"
                     /locus_tag="R8G18_001248"
     CDS             complement(15890..17170)
                     /gene="puuB"
                     /locus_tag="R8G18_001248"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HEE9795007.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17172..18662)
                     /gene="puuC"
                     /locus_tag="R8G18_001249"
     CDS             complement(17172..18662)
                     /gene="puuC"
                     /locus_tag="R8G18_001249"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HEE9795008.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18812..19369)
                     /gene="puuR"
                     /locus_tag="R8G18_001250"
     CDS             complement(18812..19369)
                     /gene="puuR"
                     /locus_tag="R8G18_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HEE9795009.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19396..20160)
                     /gene="puuD"
                     /locus_tag="R8G18_001251"
     CDS             complement(19396..20160)
                     /gene="puuD"
                     /locus_tag="R8G18_001251"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HEE9795010.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20372..21733
                     /gene="puuA"
                     /locus_tag="R8G18_001252"
     CDS             20372..21733
                     /gene="puuA"
                     /locus_tag="R8G18_001252"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HEE9795011.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21882..22046
                     /locus_tag="R8G18_001253"
     CDS             21882..22046
                     /locus_tag="R8G18_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795012.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22036..23421
                     /gene="puuP"
                     /locus_tag="R8G18_001254"
     CDS             22036..23421
                     /gene="puuP"
                     /locus_tag="R8G18_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HEE9795013.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGSVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23555..23800
                     /locus_tag="R8G18_001255"
     CDS             23555..23800
                     /locus_tag="R8G18_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HEE9795014.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24113..25756
                     /gene="sapA"
                     /locus_tag="R8G18_001256"
     CDS             24113..25756
                     /gene="sapA"
                     /locus_tag="R8G18_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HEE9795015.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25753..26718
                     /gene="sapB"
                     /locus_tag="R8G18_001257"
     CDS             25753..26718
                     /gene="sapB"
                     /locus_tag="R8G18_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HEE9795016.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26705..27595
                     /gene="sapC"
                     /locus_tag="R8G18_001258"
     CDS             26705..27595
                     /gene="sapC"
                     /locus_tag="R8G18_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HEE9795017.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27595..28587
                     /gene="sapD"
                     /locus_tag="R8G18_001259"
     CDS             27595..28587
                     /gene="sapD"
                     /locus_tag="R8G18_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HEE9795018.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28589..29395
                     /gene="sapF"
                     /locus_tag="R8G18_001260"
     CDS             28589..29395
                     /gene="sapF"
                     /locus_tag="R8G18_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HEE9795019.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29463..29816
                     /locus_tag="R8G18_001261"
     CDS             29463..29816
                     /locus_tag="R8G18_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEE9795020.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30184..30972
                     /gene="fabI"
                     /locus_tag="R8G18_001262"
     CDS             30184..30972
                     /gene="fabI"
                     /locus_tag="R8G18_001262"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEE9795021.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31117..32243
                     /locus_tag="R8G18_001263"
                     /pseudo
     CDS             31117..32243
                     /locus_tag="R8G18_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32311..34245
                     /gene="rnb"
                     /locus_tag="R8G18_001264"
     CDS             32311..34245
                     /gene="rnb"
                     /locus_tag="R8G18_001264"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HEE9795022.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34323..34426)
                     /locus_tag="R8G18_001265"
                     /pseudo
     CDS             complement(34323..34426)
                     /locus_tag="R8G18_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34480..36470
                     /gene="pdeR"
                     /locus_tag="R8G18_001266"
                     /pseudo
     CDS             34480..36470
                     /gene="pdeR"
                     /locus_tag="R8G18_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36617..36790
                     /locus_tag="R8G18_001267"
     CDS             36617..36790
                     /locus_tag="R8G18_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795023.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36880..37629
                     /locus_tag="R8G18_001268"
     CDS             36880..37629
                     /locus_tag="R8G18_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEE9795024.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37898..38116
                     /gene="osmB"
                     /locus_tag="R8G18_001269"
     CDS             37898..38116
                     /gene="osmB"
                     /locus_tag="R8G18_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HEE9795025.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38242..38568)
                     /gene="yciH"
                     /locus_tag="R8G18_001270"
     CDS             complement(38242..38568)
                     /gene="yciH"
                     /locus_tag="R8G18_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HEE9795026.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38568..39305)
                     /gene="pyrF"
                     /locus_tag="R8G18_001271"
     CDS             complement(38568..39305)
                     /gene="pyrF"
                     /locus_tag="R8G18_001271"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEE9795027.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39498..40667)
                     /gene="lapB"
                     /locus_tag="R8G18_001272"
     CDS             complement(39498..40667)
                     /gene="lapB"
                     /locus_tag="R8G18_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEE9795028.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40674..40982)
                     /gene="lapA"
                     /locus_tag="R8G18_001273"
     CDS             complement(40674..40982)
                     /gene="lapA"
                     /locus_tag="R8G18_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HEE9795029.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41131..41895)
                     /gene="pgpB"
                     /locus_tag="R8G18_001274"
     CDS             complement(41131..41895)
                     /gene="pgpB"
                     /locus_tag="R8G18_001274"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HEE9795030.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42065..42655
                     /gene="ribA"
                     /locus_tag="R8G18_001275"
     CDS             42065..42655
                     /gene="ribA"
                     /locus_tag="R8G18_001275"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEE9795031.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42719..45394)
                     /gene="acnA"
                     /locus_tag="R8G18_001276"
     CDS             complement(42719..45394)
                     /gene="acnA"
                     /locus_tag="R8G18_001276"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEE9795032.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45558..45653)
                     /locus_tag="R8G18_001277"
     CDS             complement(45558..45653)
                     /locus_tag="R8G18_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795033.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45767..45934)
                     /locus_tag="R8G18_001278"
     CDS             complement(45767..45934)
                     /locus_tag="R8G18_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795034.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45937..46101)
                     /locus_tag="R8G18_001279"
     CDS             complement(45937..46101)
                     /locus_tag="R8G18_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HEE9795035.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10925 a        12204 c        11623 g        11409 t
ORIGIN      
        1 ccgatgatcc aataaatccg gcaacaaaca tattcgctgg ttggttataa accgttttcg
       61 gcgcaccaac ttgctgaaca atcccatcct tcataatcac aatccgcgtc gccatggtca
      121 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt attcagcttc tggtgcagct
      181 tgctgatctc tgcgcgcatt tgcacgcgca gcttggcatc aaggttagag agcggttcat
      241 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
      301 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
      361 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
      421 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
      481 ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
      541 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta ccgcagcctg
      601 acggaccgac aaacacgatg aactctttat cggcaatttc caggttgaag tccttcacca
      661 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
      721 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
      781 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      841 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      901 ggcacgccca gcccggcaca gacagcgaga aagatttccg gatccggttt cgagttttta
      961 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
     1021 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
     1081 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
     1141 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
     1201 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
     1261 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
     1321 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat taccccttgc
     1381 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
     1441 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
     1501 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
     1561 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
     1621 gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc agccagatag
     1681 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1741 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1801 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1861 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1921 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1981 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     2041 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     2101 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     2161 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     2221 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     2281 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     2341 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     2401 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     2461 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     2521 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     2581 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     2641 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     2701 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2761 ctaacgcttg ctgatcgtgc gcttcgccgc catttaccgt gatacgacgt ttctgccacc
     2821 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2881 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2941 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     3001 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     3061 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     3121 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     3181 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     3241 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     3301 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     3361 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     3421 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     3481 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     3541 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     3601 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     3661 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3721 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3781 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3841 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3901 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3961 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     4021 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     4081 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     4141 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     4201 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     4261 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     4321 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     4381 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     4441 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     4501 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     4561 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     4621 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     4681 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4741 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4801 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4861 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4921 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4981 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     5041 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     5101 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     5161 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     5221 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     5281 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     5341 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     5401 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     5461 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     5521 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     5581 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     5641 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     5701 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5761 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5821 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5881 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5941 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     6001 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     6061 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     6121 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     6181 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     6241 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     6301 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     6361 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     6421 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     6481 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     6541 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     6601 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     6661 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6721 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6781 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6841 acggatatca acccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6901 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6961 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     7021 tcagtgtcgt aaatgcccag cgcggtgatg attttgaaca gcggcaccac cagcaaaatg
     7081 ccagagaaca tatacaccgt gtaaaagctg gcgttgatgg tcatccgacc tttaaagcgc
     7141 aggcgggaaa gcgcgtaagc cccaagaatg ccgaggaata ccgccaccac ggatgaaacc
     7201 acagacacca ccagactgtt acggaagtag tcgacaaacg ggaaaatcat cgggttaaaa
     7261 atgtcgacgt aatgctccag cgtccactgc tgcggcagca gcgtaggatg cagtgagatc
     7321 gcctctttcg cgcccttgaa cgaggtcatc agcatcacaa aaaatggaaa cagcgtgatg
     7381 atgagaaaca gcgccagccc gcagtaaaaa ccgatgcgac tgagtgtgcg tttatttgtt
     7441 gccattgagg ttcacccgtt tcctggtcag cagaatgaca gcgaaaataa tgatgaagag
     7501 caccaccgaa atcgccgccg ctttccctaa gtcattaaag gcaaaggcgg ttttgtagag
     7561 atagacgccg agaatatcga ccttggtggt cagcagataa acatcagcaa acatgtagaa
     7621 catccagata gtgcgcaggg tcaccaccgt cgccaggacc ggcataattg cgggcagcgt
     7681 gacgatgcga aaccgctgcc aggcattagc gccatccatc tctgccgctt cgtacagcga
     7741 tttgtcgatg gtctgcaaaa tcgccagaaa cgagataaag gcatacggga agtagcgcca
     7801 gatggcgaac agcaccacca gcacaaagct actgcccgga ttatcgaacc acagcggtgc
     7861 ctgctcatag agatgcagaa gatcgacgcc gaggtagttc acaatgccgt agccgttgtt
     7921 gaacatgtat ttccaggcaa acaccagcga aatggacggc gttacgtagg agaggatcac
     7981 cagcgaacgc gcggttttgc gcaggcggaa ctcgcggttg aaaaacatcg ccactgccag
     8041 cccgagcacg gtgctgcccg ccaccaccag cgcggtatac cagaccgtca tccacagcga
     8101 gtgccagaag ccgggatcgg agaggatacg cacatagtta ctcaccccaa caaaggttga
     8161 ctcgatgttg ggattgagcg gcagacgtaa aaaactgatt tcgatattcg acaccattgg
     8221 ccaggccacc agaccaccca gcagtaataa gctgggcgcg agaagcagca gcgcaaaggg
     8281 catatcggaa cgacctgaaa aaagcctgtt cattgcggat tcctgtcctg ggtttagtgc
     8341 tgttcgatca gttcatccag ctttttctgg ctcgcttgca gcgtgccagg gagatcggct
     8401 ttacccacgg tgacgttatg caccatggag ctcaccacgc cagaacccgt cacatcaccc
     8461 atgcgggtaa agtttttatc ccctactgcg ccaaagacct gaatatttgg cagttcaccg
     8521 attagctgat tcggtagttc acccagcgcc ttaataacgt cgttgtcttt ccaggtggcg
     8581 gtagtgacaa ccgctttatt caccggcagc gcagcacctg gcgacatcat cacccagtcg
     8641 gcaatgttgt ctgcctgctc cataaaggtg acaaattttt ctgctgcttc cgtctcttcg
     8701 gccttttgcc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
     8761 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaattac agccggaagg
     8821 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
     8881 atgatgtcgt tagaacccgg catgtcgtta gaacccggca tagtgttggc agcaaggtcg
     8941 cggtaatagg tcagtgcctg catcatctct ggtgtatcaa gggtgatttt gccttcggcg
     9001 ttaaagacgt tagcctggtt ggataacgca aactgggaga aggattgttc cgtcaacacg
     9061 ctttctgctg taggcagcgc aatgccgtat tttttattcg ccgggtcatt cagtttctgt
     9121 gcaacgtcca gtagctgttg ccagtttttc ggctcctcca gccccgcttt tgccagcaca
     9181 tctttgcgat accagatgcc gccaatccag gcgctgacgg gaacaccggt ccaggcactc
     9241 ccatcttcgg tgcgcacaat acgcagtacg ccatcgtaaa acgcgccttc accaacgttg
     9301 ctgatgaccg tggcaaccgt cttgcgatcg ataagctgct ctttatccat aactttggcg
     9361 tagtcatggc tggtttcgat cacttccggc agcgagccgc tacgtgaaag ggtgatgact
     9421 ttggtgttat aagcgtcctc ttccacgggc acttgtttaa cggtgatacc tgggttttct
     9481 ttttcaaaac gggcaatcaa tttgctgata actgcctgac gctcctgctc caccgaagaa
     9541 tgcataaatt cgatggatac atgggtttta ttttcttctt tacagcctga aatcagggcg
     9601 caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgagatg ttccttttta
     9661 tttaatccac ataacctgcc aggggcgcag cgttatttca ttacccaata gatcaacttc
     9721 actaatcaga tcccgaccat gcagattagt aatatttaca tgctgaatat ttttactgac
     9781 attaaacaat ccagtcagac aattaccatc agcgttactt ctttgaatac gcattacgga
     9841 tgaattaatc gtatcgatgg taaaattatt atccggatga aactcgttat ggctgcgacg
     9901 aagtgtaatt aaacgcgaca actcatgata taccgcatgc cttaatgtag cttcatcgtt
     9961 cagttctcgg gttatctctt tactgtgata ttttttacgg ttaatcgcac ggttatatcc
    10021 gagtttttcg acacctgcgt aatcattacg cgagccaaga atactttgaa tatatatcgc
    10081 tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg cgcaacgttc
    10141 ttcatcgcta ctctcacggc ggcttaacgc atccatatgt gagaggcgag gaagttaaac
    10201 caggtggtgt tgctggaagg tagtgtcagg ttttgcgccc acgcacaaag cgcctcaacg
    10261 ttctgttttt gcaccgcatg cagcaccagt ggcggcagcg agaactggta caccatatgt
    10321 gcttcgtcat cgcctgcgcc aaagtaagca atgttgtctt tatgcggaac attggtctcg
    10381 gtaatgatca ctgtacctgg cgcaacgtta tcaataatcg accgtaacag tttgataatc
    10441 agatgtgttt tttccagatg gatgcagctt gttcccggct ctttccacat aaagccaacg
    10501 gcatccaagc ggacatactc agcacctttc gcaaggtaac agagtaaaac atccaccatc
    10561 gccagcaaca cttcagggct acggtaattc aggtcaattt gatcgtcact aaaggtggtc
    10621 cataaatggc gcgttgaatg atcgcgcatc tggaatggcg ttaataacgg taacgcacgc
    10681 gggcgagtga cggcgctgag atcggtttgc ggatcaaccg caataaaaaa atcttcaaaa
    10741 cctggctgct gttgtaaata gtttttaaac cattcacttt ttgccgacat atggttgcag
    10801 acaaaatcaa acattaaatg actgcattca ccgagttgct gaatatcctg ccactccccc
    10861 gcttcactgg cgacctgatg ataatcaatt accgaaaacc catcatcaga tgaacatgga
    10921 taaaaaggta ataaatgaac atgtgaaaaa atgctttgca gccattgatg gtaaaactga
    10981 ttaaatgtgg gtaatggttt taaatcattg ctgtgaaatt gatcggcata ggtaattaac
    11041 acgacatcac tttcatccca gtgtttttta cgtcgctgtg taattaatcg tttcgcactc
    11101 tcaagacgcg ttacaagttt ctgtaaatga gttgcggtaa atgttccacc gtagatttcg
    11161 tccaggtaat ccgtaatttt ctgtttcatg ttgccttttt gtggtagcgg tcccatgaaa
    11221 cgagagacta ctccgcagaa aataaactgt caattgagca caagggtttt agtcacgcag
    11281 gaaatcattt tttaccaggg aaaaagcgta tttgtgagct gacgcaaata atctatgggg
    11341 atgtgaataa ggtgtgacag aaaaaaaacg gcgcataagc gccgctcatg gtgaattctt
    11401 ttaacctttg accttcggca ttgcgatgtc tttcaggcca ccggcgttct caacgtgggt
    11461 atatcccatc tcgctaagga tctcttttgc ttgccctgac tggcgtccgg cattgcaata
    11521 cactttcacg gtgtcgtttt tatccggaac ggcggtggca atgcgctctt tcacttcctt
    11581 caggggaata ttgatggccc cctgaacgtg ctcttgctga tactgctctg gaacacgaac
    11641 atcgatccag tgttcagcgg cgaaaacggg cagtgaaaac accagtgcca gagcaagtaa
    11701 gcctttttta aacatgtaag cgtccttcta tggatgaaaa ttgccatcca ttatacgcct
    11761 gagatttaac aatttttgcg aaactcccct caccttttat aacgctgtgc cagtttatta
    11821 gcagcccgac ttaacagcgg ttccagcgcc acagccagca acattttcag cggtcggcga
    11881 gcaactgatt ttatcgccca gcccgctaca cccgccgggc catagcgcag tgcggtaaga
    11941 agtaccagct tgcctgctaa tttgaaacca ggctttacct tttgcccggc ctgttgccag
    12001 cgagtattca taactttcct cacagttgac ggaaacggct acgtaacgtg aaagtatcgg
    12061 aagtgacata acgttccatc tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga
    12121 cttcatccag caattcgctg ctggaaggta gctgctcacc aaaggccatg ttgtccggca
    12181 ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc gccagaccga
    12241 agaaaatcga cagcaccacc aggatacgca ccagttttac cggtacatca aaatagttgg
    12301 caatcccggc gcagacgccg cggaccatgc cctgctgtgg aatacgccat aattttttat
    12361 tgagattaat gcccgccatt agcgatccct ccagttcgga tgttctgcat caagaataga
    12421 ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt gcgctaatcg
    12481 ctgctgctca ctttgcgaca attcatcgcg accagaacga ttgctgtaat gcagccataa
    12541 ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga atagccagaa atagcgcgct
    12601 cataagtact ccttagacga tgagcggcgg cgcgttcgga cgccgcctgg atgttattat
    12661 tgattgtctt gcttcatttt ggcttttaat tgtgccagtt gttcgctgat tgcatcatcg
    12721 gctttcagtt cggcaaactg atcgtccagc gatttttgtt taccgaagct gtggctttct
    12781 gcttccgctt ccatctggtc aattcgacgt tcgaaagatt cgaaacgagc cattgcttca
    12841 tccagtttgc cactgtccag ctgacgacgc acatcgcgcg acgagtttgc cgcctgatga
    12901 cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca atttgttttc cagctcgcca
    12961 atctctttct tcatgcgtgc cagcgtatcg tccaccagcg tcacttcatg ttccagggac
    13021 ttaatcagat cggtcagttt ctgtttttca attaacgctg cacgtgccag atcctctctc
    13081 tctttcagca gcgccagttc ggctttttcc tgccattcaa cttcacgcgc cgacgcttgt
    13141 tcaatacggc gagtcagctg tttcttttct gccaacgcac gcgccgaagt agaacgtact
    13201 tcaaccagtg tatcttccat ctcctggatc atcagacgca ccagtttctg tggatcttcc
    13261 gctttctcta acagagcgtt gatgttggca ttcacgatgt cggcaaagcg agaaaaaata
    13321 cccataatgt tgtcctcttg atttctgcgt tcaggattgt cctgctgaac tgttaataca
    13381 atttgcgtgc caatttttta tctttttgat ttataaagat ctgattgaag aatcaacagc
    13441 aacatgccag gatgaattag cgaattacac taacaagtgg cgaatttcat catggccgaa
    13501 tacaaagata atttacttgg tgaggcgaac agctttctcg aagtgctgga acaggtttcg
    13561 catctcgcac cgctggacaa accggtgctc atcatcggcg aacgcggcac cggtaaagag
    13621 ctgattgcca gccgcctgca ttatctctcc tcccgttggc aagggccgtt tatttccctt
    13681 aactgcgcgg cgttaaatga aaatctgctg gattccgaac tgtttggtca cgaagcgggg
    13741 gcgtttaccg gtgcgcaaaa acgtcatcca gggagatttg aacgtgccga cggcggtacg
    13801 ctatttcttg atgaactcgc tacggcaccg atgatggtgc aggagaaatt attgcgcgtg
    13861 attgagtacg gtgaactgga gcgcgttggc ggcagccagc cattgcaggt gaatgtgcgg
    13921 ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca atgaaggcac ttttcgcgct
    13981 gacctgctcg accgactggc ttttgatgtt gtacaactgc caccactgcg cgagcgcgaa
    14041 agcgacataa tgttgatggc agaacacttt gccatccaga tgtgtcggga aatcaagctg
    14101 cctctgttcc cggggtttac ggagcgcgcc agagaaacat tgctgaatta tcgttggccg
    14161 ggaaatattc gtgaattgaa aaacgcggtg gaacgttcag tgtatcgcca cggcaccagc
    14221 gattatccgc ttgatgacat cattattgat ccctttaaac ggcgtccgcc tgaagacgct
    14281 atcgccgttt cagaaaccac ctcgcttccg acgctgccgc tggatttacg tgagtttcaa
    14341 atgcagcagg aaaaagagtt gctgcaactc agtttgcaaa aggggaaata taaccagaag
    14401 cgcgcagctg aattgctggg gttaacctac catcagttcc gcgcgttgtt gaaaaaacac
    14461 cagatttagc gaagttgggc aatgcgattc gccggatgcg gcgtgaacgc tttatccggc
    14521 atacataatc aacgtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt
    14581 gggctattaa tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc
    14641 cgggatggtc agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag
    14701 gagcagcccc tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc
    14761 gcccgtttgc ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc
    14821 aatggccgga acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg
    14881 attcgcgcgt tcgcagagtg attctttgtt gataatattg agcaccgcgt gcgcggcagc
    14941 caccgccagc gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat
    15001 aatattcgcg ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat
    15061 cgtcattaag tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc
    15121 aaagccgctt tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg
    15181 aatggcggca accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc
    15241 gaaaataatg gccgcgacct gcttcgcttc gatgtctgat ttaaacaagc gttcgatggc
    15301 gtcgagggag tcctatgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac
    15361 cgaaccaggg aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat
    15421 ggtcatatat gtacgaccgt ggaagccacc gctaaacgca atcacgccag ggcgtccggt
    15481 atgggcgcga gcaattttca ccgcgttttc caccgcttcc gcaccggtag tgaagaacgc
    15541 ggttttggcc tgcccgctca ccggggcaag ggcgttgatt ttctccgcca gggtgacgta
    15601 gctttcatac ggcacaatct gatacgcggt gtgggtaaac tgttgcagtt gctgctccac
    15661 cgccgcgacc agatcagggt ggcgatgtcc ggtattcagc accgcaatgc ctgcggcgaa
    15721 atcgatgtac tcgttgccct caacatcctt cagagtagcg ttttcagccg actgggcgaa
    15781 gaagttacac atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc
    15841 attgttgctc atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca
    15901 gacgatcgcg caggctgtac caggctgcgc ccatcgcggt aaacggtact cgcagcgtgc
    15961 gcccgccggg gaacgggtaa tacggcagat tggcgaaggc atcgaaacgt tcggcgtcgc
    16021 cgcgcagcag ttcggcaatc aaacgtccgg cgagatgagt acaggtgacg ccgtggccgc
    16081 tgtaaccctg catgtaatag atgttggtat caaggcgacc aaactgcggc atgcgcgaca
    16141 gggtcagcag gaagttgccc gtccagcggt agtcgatttt gacgcccttc agttgcggga
    16201 aggttttcag cagtttcggc acaacaagac gctcaacgtc atccgggtcg cgcgcgccgt
    16261 agaccacgcc gccgccgtac agcaaacggt tgtcggcggt aagacggtag taatcaagca
    16321 gataattaca gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg
    16381 acagccgttc agtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gccagttccg
    16441 gctctacttt atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct
    16501 gacctttggc agtgcgcacc accgctggcg tggtgtgctg gatctgcgtc acggcagaaa
    16561 gctcatacac gcgcccgccg ttcaggcgga tggcatcggc ttcaccaatg gcgaggttta
    16621 gcgggtgaat atgcccaccg ctgtgatcca acagtgcgcc agtgtagcga tcgctggcga
    16681 cctcgcggcg aatggcgtta gcgtccagca gctccagctg tttattgccg tagcgttccc
    16741 agttctcttt ctgctcttcg agtgtggcga gctgtttatc attcatcgcc acaaacaggc
    16801 cgccggggcg gtagtcgcag tcaatctgat agcgtttgat acgttcgcgg atgatctcgc
    16861 caccctcgaa catcatgctg ccgagcatac gggcggtgtc catgccgtag cttttttcga
    16921 tcacgtcgat gtcgcggctg taggagttca caagctgccc gccattgcgc ccgcttgcgc
    16981 caaagccgat gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca
    17041 gcgcggagga gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact
    17101 cattcagtgt gtcgaatggt gcatatttat tcgcactggc ggcgtagtaa ctgctggtat
    17161 gttcggtcat ttcaggcctc caggcttatc cagatggttt tcagttcagt gaatttttca
    17221 agggcatgca gggatttgtc gcgaccgttg ccgctctgct tatagccgcc aaacggcacg
    17281 gtcatatcgc cgtcgttgta gttattgacg aagacggaac cggctttcag gcggcggctc
    17341 atacggtgcg cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg
    17401 tcgttggcaa gctgtagcgc ctggtcttct gatgtgaaac gcgtgaccac cagcaccgga
    17461 ccgaaaatct cttcgcggct taaggacgca ttcgggtcaa catccacaaa gatggtcggg
    17521 ccgatggcgg cagccagctc agcgttacga ccatccagca gcagttgccc tttgctttcg
    17581 ccttcacgaa taaagctatg gaccgagtcg gcgtgggcgc aatcgattaa ggtgcccatg
    17641 gtggttacgg tatcaagtgg atggcccggc tgccagtttt gcgcctgctg ttttaacagg
    17701 gctaagaatt catcggcgat gctctcttcc agtaacaaac gcgttccggc gatgcacacc
    17761 tggccctggt tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg cagatccgga
    17821 caatcggcga aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac gcgtttcatg
    17881 ttgctatcac ccgcgtcttt cagcagttgt ttacccgtac gcgtggagcc ggtaaaggcg
    17941 atggcatcga tatcgttatg acgcgaaagc gcctgcccgg cttcatgacc ataaccgctc
    18001 actacgttca gcacgccatc cggcagcccc gcttcttctt ttgccagccc ggcgagacga
    18061 atcgcactga gtggtgattt ttcagacggt tttagaatca cgctgtttcc cgccgccagc
    18121 gccgggccga gtttccagca agtcagcagc agcgggaagt tccacggcac gatggcggca
    18181 atcacgccga ccggttcacg cacgatcatc gccagctcat ggctactggt ggtcgccact
    18241 tcgccataca ctttgtcgat cgcttcggcg taccagcgaa tggcgcgcgc cgcgccggga
    18301 atatcatcac gcagactgtg acgaatcggt ttgccggtgt cgagagtttc cagcagtgcc
    18361 agctcttcgg cgtgggcttc cattaaatcg gcgagtttat tcagtaccgc tttacgttta
    18421 gccggagaag agagtgacca gtcgccgcgt tcaaatacgc cgcgtgctgc gctcaccgca
    18481 tggtcgatat cgacgctctt gccgcgggca attttcgcca gcggtgcctg ggtgaccgga
    18541 tcaacggttt caaaggtttc attttccgcc gcagcagtat attcaccgtt aataaataag
    18601 cggttttcaa tggcgagact taacgcttta tcctgccagt aagccagatg atgaaaattc
    18661 attatgactc ctgtttcacg tctatcagat atatgcagtt tgtgaacgtc agggcgcaga
    18721 gatccccggg ccgcatgccc gtttatttct ggcgacattc tttatggatt gccgggcgca
    18781 ttgcgcttac ccggcttaca gaacaaaaag attaaaacgt ggtgggcgta tgggcgctga
    18841 taattcggca aatacctgcc gacgtattac tgaaactgtg cgggatgccg gtattaatgg
    18901 cataactttg ccccgcgacg aggtggtaat cctgaccatt aatcgtcaga acaatttcac
    18961 cttccagtac tgtgcctatt tcctcaccct gatgcttaat tctttcccca gtggttgtgc
    19021 ccggctggta cgtttcaaag atcatcgcca gcgtgcgatt cgggttaccg ttatgaacca
    19081 gcttcattga cacaccctga ctgcccatct caattaagtc gtcctgatta atgacgacct
    19141 gcggctcatc aggtttttcc ggctcggaaa agaattccga gagtgacaga ccatacacct
    19201 tcagcagctt ttgcagcgta ctgatggcag ggctgacttt atcttgttct atcgtactga
    19261 tagcactgtg agtcagcccg gagagttcgg cggcacgacg ttgtgaaagc ccctgctgct
    19321 ggcggatttc cgacaagcgt tttcctggcg ccagtccctc atcactcata tttgcatttc
    19381 cttaaactgt agtggtcaga gtcgctgttt ttcagcgata tggtgctgac aagcggtgat
    19441 aaagccctcg aacaatatac gcgaaagcgc gtactcgcta ctgttccatt ccgggtgcca
    19501 ctgtacgccc agcgcaaaag gatgattgat gacgctaacc gcctcaacta agccatccgg
    19561 cgagcgagct tcaacacgca accgtgggct aacgaccttc gccccttgtc catgtagaga
    19621 gtttacccaa aagttgctac attcaggtaa caacgcagac agtaatcccc cctcttcaac
    19681 ctgaacttca tgcgacggtg catattgctg ttccaccggc agttcgggat cttcccgatg
    19741 ttccagcaat tcaggctgtt cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac
    19801 cagttcttgt aaaccccggc agatggcgaa aatggggatg cgcctttcga gtgcggcatt
    19861 aattatcgcc atgctcagaa gatcacgccc gggatcggcg tcaggctcat cgccgttttc
    19921 accatatagg tgcggctgca cattgctggg actaccagga agataaatgc catcgagttt
    19981 cggcaaaagt tgttcaagta acgacggttc cgccagcgca tgtggtagcg caattggcaa
    20041 gccgcctgcg tggatgatgg cattcaggta cttttcttgc agagtctggg tcgcatgacc
    20101 cttaagcctg ttcctgcaca ttacgacacc gataaccgga ttgttcatta tattttccat
    20161 gctcaatctc acaaagtgga ctaaattatc gccattactg cgctttaaat gacctttatg
    20221 ttcaatattt tttcaatcta gcagtgggtt ttcatttttg caaactcaat ttaacatttg
    20281 acaaacattt agtttgcata cagattcgaa tggtggtcat tatattttac gctttgataa
    20341 cgagcggaaa acaaaccaaa ggcgaagaat catggaaacc aatatcgttg aagtagagaa
    20401 ctttgttcag cagtcagaag agaggcgggg tagcgccttt acgcaggaag tgaaacgcta
    20461 cctggagcgc tacccgaata cgcaatatgt tgatgtttta cttactgatt tgaatggttg
    20521 cttccgtgga aaacgtatcc ctgtttcaag cctgaagaag ctcgagaaag ggtgttattt
    20581 cccggcctcg gtatttgcaa tggatattct gggcaacgta gtagaagaag caggtctggg
    20641 tcaggaaatg ggcgagccgg atcgtacctg tgttcctgtt ctcggttcct taactccttc
    20701 tgccgccgat ccagagttta tcggtcagat gctcctgacc atggtcgatg aagatggcgc
    20761 tccctttgac gttgagccgc ggaacgttct taaccgcctg tggcagcagc tgcgccagcg
    20821 cgacgctgaa gggaatctgc aaccgccctg cgcgcctggc accgatgacc gcaatacaca
    20881 aagccaggtt tactccgttg ataacctcaa ccacttcgcc gacgtgctca atgatattga
    20941 tgaactggcg cagttgcagc tgattccggc agatggcgcg gtcgctgagg cctcgccggg
    21001 tcagtttgaa atcaaccttt accatactga taacgtgctg gaagcctgcg atgatgcgct
    21061 ggcactaaaa cgcctggtgc gtctgatggc aggaaagcat aagatgcacg ccacttttat
    21121 ggcgaagccg tatgaagagc acgcgggcag cgggatgcat atccatatca gtatgcaaaa
    21181 taatcgtggc gagaatgtgc tttctgacac agaaggcgaa gattcgccgc tgctgaaaaa
    21241 gatgctcgcc gggatgattg acctgatgcc gtcgtcgatg gcgttgctgg caccaaacgt
    21301 gaactcgtat cgccgcttcc agccgggaat gtatgtgccg acgcaggcgt cgtggggcca
    21361 taacaaccgc accgtcgccc tgcgtattcc gtgcggcgac cgtcataatc accgcgtgga
    21421 atatcgcgtg gcgggtgccg atgccaaccc atatctggtg atggcagcga tttttgccgg
    21481 tattttgcat ggccttgata acgagctgcc gttgcaggaa gaagtcgaag gcaacgggct
    21541 ggaacaggaa ggcttaccct tcccgattcg ccagagcgat gccctgggtg agtttatcga
    21601 gaatgatcac ctgcgccgct atttaggcaa acggttttgc catgtgtatc acgcctgtaa
    21661 aaatgatgag ctgttgcagt ttgagcgcct catcacagaa acagaaattg agtggatgtt
    21721 gaaaaacgcg taattcccct tgcggggttg tttcatcggc ctgagcggag ccaggcctga
    21781 catttgctgg taatccagac atttcccgga tgtcgcgtta tggagcgcag gcggcaacgg
    21841 gcttccgtta acgattaaga ctgtgacgag gaaatgagat gatgccgatg attaaatccc
    21901 cgcatggcga gggaggctgc gtatgcgccc ctcctgctac tgactggact ccgccccctt
    21961 tgcttccctt gctaaacagg tttgatttca ggtcgacacg accgcaaacc ttattacgca
    22021 ggggaggcag caattatggc tattaattca ccactgaata ttgctgcgca acccggcaaa
    22081 acccgtctgc gaaaatcact gaaattgtgg caggtggtaa tgatgggtct ggcctatctc
    22141 acgccgatga ccgtatttga tacttttggt attgtctccg gcattagcga cggtcacgtt
    22201 ccggcgtcct atttgctggc gctggcgggc gtgctgttta ccgctatcag ctacggcaaa
    22261 ctggttcgcc agtttccgga ggccggttcg gcctatacct acgcgcaaaa gtcgattaac
    22321 ccgcacgtcg gatttatggt cggctggtca tcactgctgg attatctctt tttgccaatg
    22381 atcaacgttt tgttggcgaa aatctatctc tccgccctct tcccggaagt gccgccgtgg
    22441 gtgtgggtgg taaccttcgt cgccatttta accgccgcga atctgaagag cgtcaacctg
    22501 gtcgctaact tcaataccct gtttgtactg gtgcaaatct ccatcatggt ggtgtttatc
    22561 ttcctggtgg ttcagggact gcataaagga gaaggcgttg gcaccgtctg gtcacttcag
    22621 ccgtttatca gcgagaacgc gcacctgatc ccgattatta ccggtgcgac gattgtctgt
    22681 ttctcgttcc tcggtttcga tgcggtgacc acgctttcgg aagagacgcc ggacgccgca
    22741 cgcgtgatcc cgaaagccat cttcctgacg gcggtatacg gcagcgttat ctttatcgcg
    22801 gcgtcgttct ttatgcagct gttctttccc gatatcagcc gctttaaaga cccggacgcc
    22861 gcactgcctg aaattgcgct gtacgtcggc ggcaagctct ttcagtcgat tttcctctgc
    22921 accacgtttg tgaacacatt agcgtctggc ctggcttcac atgccagcgt gtcgcgtctg
    22981 ctgtatgtga tggggcgtga caatgtgttt ccggagcgcg tgtttggcta tgtgcaccca
    23041 aaatggcgga ctccggcact caacgtcatt atggtcggga ttgtcgcgct gtcggcgctg
    23101 ttctttgatt tagtcaccgc gacagcactg attaacttcg gtgctctggt ggcgtttacc
    23161 ttcgtgaatc tgtcggtgtt taatcatttc tggcggcgta aaggaatgaa taaaagctgg
    23221 aaggatcact tccactattt gctgatgccg ctggttggcg cgctgacggt gggtgtgctg
    23281 tgggttaacc tagagtcaac gtcactgaca ctcggtctgg tatgggcttc gttaggcggc
    23341 gcatatttgt ggtatctgat ccgccgctat cgcaaagtgc cgctgtacga aggtgacaga
    23401 acgccggtga gcgaaacgta atagccggat gcgcggcgca ttatcctgcc tgagggtaac
    23461 gcgccgcgcc attaactata aaaagatgta tgacccggat cacgagtgct atcatccgcc
    23521 cttatgctta gcgcattaac cccaggggca aataatgaac cacgatatcc cgctaaaata
    23581 ttttgatatt gccgatgagt acgcgaccga atgcgcagag cctgtcgcag aggctgaacg
    23641 cacaccgctg gcccactact tccagttact gctcacccgt ttgatgaaca atgaagagat
    23701 cagcgaagaa gcccaacatg aaatggctgc tgaagcgggg attaatcccg tacgcattga
    23761 tgagatcgca gagtttttga atcaatgggg taatgagtaa ttatttaatt gcagacgatc
    23821 ggtcagtatc gttgtccgga taaagcgttc acgccgcatc cggctaacaa tgacacgcac
    23881 tgcagcgtgc tttaatgggt ttctcacgat cgtaccgctc tgctcctcgc taagttgttc
    23941 tgtgagttat agttatagag aaatcacggg tacgaatgtt gaacagatgg cgacgccacc
    24001 tgttcaacga ccaaccacac ggccctcatt acgacccctg aacacaaacg gtaatggcaa
    24061 atgtcattaa atattagtaa gatagcctct ttacataaaa tcccttaata ttatgcgcca
    24121 ggtattatcg tctcttttgg tgattgctgg acttgtgagt ggtcaggcaa tcgccgcgcc
    24181 tgaatccccc ccgcatgctg atatccgcga cagcggtttt gtctattgcg tcagcgggca
    24241 agtcaacacc tttaacccat ccaaagcgag cagtgggtta attgtcgata cccttgccgc
    24301 ccagttttat gatcgactgc tggatgtcga tccctatacc tatcgcctga tgccggaact
    24361 tgccgaaagc tgggaagtac tcgacaacgg cgcgacctat cgcttccacc tgcgtcgcga
    24421 tgttccgttt caaaaaaccg actggtttac tcccactcgt aaaatgaatg ccgacgatgt
    24481 ggtgtttacc ttccagcgaa tttttgaccg caacaacccg tggcataacg tcaacggcag
    24541 caacttcccc tacttcgaca gcctgcaatt tgccgataac gtgaaaagcg tccgcaaact
    24601 ggataatcat accgttgagt tccgactcgc ccagccggat gcttcttttt tgtggcacct
    24661 cgcaacccat tatgcttcgg tcatgtcggc agaatatgcc cggaagttag agaaagaaga
    24721 tcgccaggag caactcgacc gtcaaccggt cggcaccggt ccgtatcagt tgtcggaata
    24781 ccgcgccggg caatttattc gcctacaacg tcatgatgac ttctggcgcg gtaaaccgtt
    24841 aatgccgcag gtagtggtgg atttaggctc cggcggcacc ggacgtctgt cgaaactcct
    24901 gaccggggaa tgcgacgttc tggcctggcc tgctgccagc cagctatcca ttttgcgtga
    24961 cgacccgcgc ttgcgtttaa cgctgcgtcc tgggatgaac gtcgcctatc tggcatttaa
    25021 caccgccaaa ccgccgctaa ataatcccgc tgtccgccat gcactggcac tggcgattaa
    25081 taaccagcgc ctgatgcaat ccatctatta tggtacggct gaaacggcgg cctctatttt
    25141 accgcgcgcc tcgtgggcct atgacaacga ggctaaaatt actgaataca atccggcgaa
    25201 atcgcgcgaa cagttgaagg cgttggggct ggaaaactta acgctgaaac tgtgggtgcc
    25261 cacacgttcg caggcgtgga accccagtcc actgaaaact gccgaactga ttcaggcgga
    25321 tatggcgcag gttggcgtaa aagtggtgat tgtgccggta gaaggtcgct ttcaggaggc
    25381 gcggttgatg gatatgagcc atgatctgac gttatccggt tgggcgacgg acagtaacga
    25441 cccggacagt ttcttccgtc ctttactgag ctgcgcggca attcattcac agaccaacct
    25501 cgcccactgg tgcgatccga aattcgacag cgtgttgcgt aaggcgctct cctcgcagca
    25561 gctggcggcg cgtattgaag cctatgacga agcgcagagt attctggcgc aggaattacc
    25621 tattctgccg ctggcgtcgt cattgcgttt gcaggcctat cggtacgata tcaaaggtct
    25681 ggtacttagc ccgtttggta acgcctcctt tgctggggtg tatcgcgaga aacaggatga
    25741 ggtgaaaaaa ccatgattat cttcacctta cgccgcattt tgttattgat tgtcaccttg
    25801 ttcctgctga ccttcgttgg cttcagcttg agctatttca cccctcatgc gccgttacaa
    25861 ggcgcgtcac tgtggaatgc ctgggtgttc tggtttaacg gcctgatcca ctgggatttt
    25921 ggtgtttcca gcattaacgg tcaaccgatt gccgaacagt taaaagaggt cttcccggcg
    25981 acgatggaac tgtgcatcct cgccttcggt tttgcgctga ttgttggcat ccccgtgggg
    26041 atgattgccg ggattacgcg ccataagtgg caggataact tgatcaacgc catcgcctta
    26101 ctgggctttt caatcccggt gttctggctg gcgcttctgt tgacgctgtt ttgttcactc
    26161 acgctaggtt ggttgcccgt ttcagggcgt ttcgatctgc tctacgaagt gaaaccgatt
    26221 accggttttg cgttgattga tgcctggctt tcggactcac catggcggga tgaaatgatc
    26281 atgagcgcaa tccgccatat gatattgccc gtgatcaccc tgtcggttgc gccaacaact
    26341 gaagtgatcc gcctgatgcg tatcagcact atcgaagtgt atgaccagaa ctatgtaaaa
    26401 gcggcggcga cccgcggttt gtcgcgcttt actattttgc gtcgccacgt tctgcataac
    26461 gcgttaccgc cggttattcc tcgcctgggc ttacagtttt ccaccatgct gacgctggcg
    26521 atgatcaccg aaatggtttt tagctggccg ggtttagggc gctggttaat taacgccatc
    26581 cgccagcagg attacgcagc catttccgcc ggagtgatgg tgtgtggctc actggtcatt
    26641 attgttaacg tgatttctga tattttgggt gccatggcta accctctgaa acataaggaa
    26701 tggtatgcct tacgatagcg tatacagcga aaagcgcccg ccgggcacgc tgcgtaccgc
    26761 ctggcgcaaa ttttatagtg acgcctctgc gatggtcggc ctgtacggct gcgcggggct
    26821 ggctgtactg tgtatttttg gcggctggtt tgcgccttac ggcatcgacc agcaatttct
    26881 cggttatcaa ttattgccgc catcatggtc gcgctatggc gaagtttctt tcttcctggg
    26941 gactgacgac ctcggacgcg atgttttaag ccgtttactg agcggagctg cgccaaccgt
    27001 gggtggcgca tttgtggtga cgcttgccgc gacgatctgt ggcctggtac tgggcacctt
    27061 cgccggggcg acacacggcc tccgctcagc ggtgcttaac catattctgg ataccttact
    27121 ggcgatccct tcgctgctgt tggcaattat cgttgtagcg tttgccggac cgagtttgtc
    27181 ccacgctatg tttgctgtct ggctggcatt gctgccgcgt atggtacgtt cgatttacag
    27241 catggtgcat gacgaactgg aaaaagagta cgttatcgcc gcccgtctgg atggcgcatc
    27301 aacgctgaat attctctggt ttgctgtgat gccaaacatc accgccgggc tggtcaccga
    27361 gatcacccgc gcactgtcga tggcaattct cgatatcgcc gcgctgggct ttctcgatct
    27421 cggcgcacaa ctcccctcgc ctgaatgggg agcgatgctc ggtgatgcgc tggaactgat
    27481 ttatgtcgcc ccgtggactg tcatgctgcc aggtgcggca attatgatta gcgtgttgtt
    27541 ggttaacctc cttggtgatg gcgttcgtcg tgcgattatt gcgggggtgg aataatgcca
    27601 ttactcgata ttcgtaacct gaccattgaa tttaaaaccg gtgatgagtg ggttaaagcc
    27661 gtcgaccggg taagtatgac cttaaccgaa ggtgaaatcc gcggtcttgt tggtgaatcc
    27721 ggttccggca agagtttgat tgcgaaagcg atttgtgggg tgaataaaga taactggcgt
    27781 gttactgctg accgtatgcg ttttgatgat atcgatttgc tgcgtctctc cgcacgcgaa
    27841 cggcgcaaac tggttggcca taacgtgtcg atgattttcc aggaaccgca gtcgtgtctt
    27901 gacccttcag aacgtgtggg tcgccagttg atgcaaaaca tcccagcctg gacctataaa
    27961 ggccgttggt ggcagcgttt tggctggcgc aaacgccgtg ccattgaact gctgcaccgc
    28021 gtggggatta aagatcacaa agatgcgatg cgcagttttc cctatgagtt gaccgaaggt
    28081 gaatgtcaga aagtgatgat agccattgca ctggcgaatc aaccgcgtct gctgattgct
    28141 gacgaaccga ccaactcaat ggagccaaca acccaggcgc aaatctttcg cctgctgacg
    28201 cgtctcaacc aaaacagcaa taccactatt ttgcttatca gccatgactt acaaatgctt
    28261 agccaatggg cggataaaat taacgtgctt tactgcggtc aaacagtgga aaccgcgccg
    28321 agtaaggagt tggtgacgat gccacatcat ccttataccc aggcgctgat ccgcgcgata
    28381 ccagacttcg gcagcgcgat gccgcataaa agtcgcctca atacgctgcc cggcgctatc
    28441 ccactgctgg aacagttacc gattggttgt cgtctggggc cacgttgccc gtatgcacaa
    28501 cgagaatgca ttgtgacgcc acgtttgacg ggggcgaaaa atcatctcta tgcctgtcat
    28561 ttcccgctga acatggagaa agagtgagat gatcgaaacg ctgcttgaag tgcgtaattt
    28621 aagtaaaacg ttccgctacc ggaccggctg gtttcgtcgt cagaccgtag aagcggtaaa
    28681 acccttgagc tttacgctac gtgaaggcca gacactggcg attattggcg agaatggttc
    28741 gggtaaatcc acgctggcaa aaatgctggc gggaatgata gagcctacca gcggcgagtt
    28801 attgattgac gatcatccac tgcattttgg cgattattcc ttccgcagtc agcgtattcg
    28861 catgattttt caggatcctt cgacctcgtt gaatccccgt caacgtatct cgcaaattct
    28921 ggattttcca ctgcgcctga acaccgacct ggaaccggaa cagcgtcgca aacagattat
    28981 tgaaacgatg cgtatggttg ggctgctacc ggatcacgtc agttactatc cgcatatgtt
    29041 ggcacccgga caaaaacagc gtctgggtct ggcgcgcgcg ctaatattgc gcccaaaagt
    29101 cattattgcc gatgaagccc tcgcttcact ggatatgtcg atgcgttcgc agttgattaa
    29161 tctgatgctg gagttacagg aaaaacaggg catttcgtat atttatgtta cccagcatat
    29221 cggaatgatg aagcacatta gcgaccaggt gctggtgatg catcagggcg aggttgtcga
    29281 gcgaggcagc accgcggatg tgctggcatc gccgctgcat gaactcacca aacggctgat
    29341 tgccgggcac tttggcgaag cattgacagc ggatgcgtgg cgtaaagatc gctaattttc
    29401 tgaatcctcc ttccagatta tccacctcat tctgacaaga gattaaatct tgtcagaatg
    29461 aggtgatgga taaaataaaa tcaaatgcgc gtgatttaag acgcaatctc actttgcagg
    29521 aacgaaagct ctggcgatat cttcgcagcc gacgttttgg tgatttcaaa tttcgccgtc
    29581 aacatccggt ggggagctac attctcgatt ttgcttgctg ctcggcgcgc gtagtcgttg
    29641 agctggatgg tgggcagcat gatttggcag ttgcctatga taccaggcgc actagctggc
    29701 ttgagtcgca gggctggacc gtgctgcgtt tctggaataa cgagattgat tgtaatgagg
    29761 aggcggtgcc ggagattatt ctgcaggaac tgaaccgccg atcaccctca ccctgaaagg
    29821 gcgagggggc cgaccgagcc gaatagctgt tgtggtgaaa acatggagac agtgctggag
    29881 aatattcggc aaggtctgaa ccatcccagc catcgccata aaagggttag gggctgtatg
    29941 agcctgtttg ttgctggggt aacaatattt gcacaatatg gtcccctcgc ccctctgggg
    30001 agagggttag ggtaagggga aaagcgccca ccctgccgca gcctgctccg gtcggacctg
    30061 gcaactatag ctactcacag ccaggttgat tataataacc gtttatctgt tcgtactgtt
    30121 tactaaaacg acgaatcgcc tgattttcag gcacaacaag catcaacaat aaggattaaa
    30181 gctatgggtt ttctttccgg taagcgcatt ctggtaaccg gtgttgccag caaactatcc
    30241 atcgcctacg gtatcgctca ggcgatgcac cgcgaaggag ctgaactggc attcacctac
    30301 cagaacgaca aactgaaagg ccgcgtagaa gaatttgccg ctcaattggg ttctgacatc
    30361 gttctgcagt gcgatgttgc agaagatgcc agcatcgaca ccatgttcgc tgaactgggg
    30421 aaagtttggc cgaaatttga cggtttcgta cactctattg gttttgcacc tggcgatcag
    30481 ctggatggtg actatgttaa cgccgttacc cgtgaaggct tcaaaattgc ccacgacatc
    30541 agctcctaca gcttcgttgc aatggcaaaa gcttgccgct ccatgctgaa tccgggttct
    30601 gccctgctga ccctttccta ccttggcgct gagcgcgcta tcccgaacta caacgttatg
    30661 ggtctggcaa aagcgtctct ggaagcgaac gtgcgctata tggcgaacgc gatgggtccg
    30721 gaaggtgtgc gtgttaacgc catctctgct ggtccgatcc gtactctggc ggcttccggt
    30781 atcaaagact tccgcaaaat gctggctcat tgcgaagccg ttaccccgat tcgccgtacc
    30841 gttactattg aagatgtggg taactccgcg gcattcctgt gctccgatct ctctgccggt
    30901 atctccggtg aagtggtcca cgttgacggc ggtttcagca tcgctgcaat gaacgaactc
    30961 gaattgaaat aatcattctg ttggtaaata ttgggcggcg ttctgccgcc cgttatctct
    31021 gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt
    31081 caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc
    31141 aaaagccact ggtatcatga aacgcaatcc agtactgcgg agtatgacgt tctgcctctg
    31201 gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagaa
    31261 acgctggctc ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggaattgttc
    31321 cctgaccagc ttaccgtgac ccgttcacag accttcaccg cttatgaacg cttgtctacg
    31381 gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga
    31441 cttacgccgc tccccgggcc tgattcctcc agggaaagca atcataggtt ggcacaaatc
    31501 acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag
    31561 catctgaatg acgtcggtct tactgcctgg gactgtgtga tcattaacca aatcattggt
    31621 tttattggct ttcaggcgcg gaccattgcg acatttcagg cttatctcgg gcacccggta
    31681 cgctggttac ccggtctgga aatacaaaac tacgccgacg cgtcactgtt tgctgatgaa
    31741 tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagagta cacaaaaagt
    31801 tcaacagcag aactttgcca actggccgaa acactctctc tccaccctat ttcactttcc
    31861 cttctcgaaa ggttgttaaa cagtacacgg gttaatacac agccggataa taagcttgcg
    31921 gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt ttgccgcctg tatggattca
    31981 tcaaatgaat ataaaaaatc agcccccttc tgcgcaaggg cgaaaatgaa attaaccaat
    32041 gggctgaccg tcattctgtt gagcacgcta ccgttcaggc gatacaatgg ctgacccgag
    32101 cacccgatcg ctttagcgcc gcccagttca gccctttact cgaacacgaa aaatcatcaa
    32161 cgcagattat taatctgctg gtatggagcg ggctgtgtgg ctggataaat cgcttaaaaa
    32221 tcgcgttggg tgagacatat taaccttgcc gcgtcagaca gattcgcgta aaactgtcag
    32281 ccgctctaat ggccaccaaa atagacaatt atgtttcagg acaacccgct gctagcgcag
    32341 cttaaacagc aactgcattc ccagacgcca cgcgctgaag gggtggtaaa agccacagaa
    32401 aaaggctttg gcttcctgga agtcgacgcg caaaaaagtt atttcattcc gccgccgcag
    32461 atgaaaaaag tcatgcatgg cgaccgaatt atcgcggtga tccacagtga aaaagaacgt
    32521 gaatccgcag agccagaaga actggttgaa ccgttcctga ctcgtttcgt gggtaaagtt
    32581 cagggcaaaa atgaccgtct ggccatcgtt cctgatcatc cactcttaaa agacgccatt
    32641 ccttgccgcg cagcccgtgg cctgaaccac gagtttaaag aaggcgactg ggcggttgcc
    32701 gaaatgcgcc gtcatccgct gaaaggcgat cgttctttct atgctgaact gacacaatac
    32761 atcacttttg gtgacgacca ctttgtaccg tggtgggtta cccttgcgcg ccataatctg
    32821 gaaaaagaag caccagacgg cgtcgctacc gaaatgctcg atgaaggtct ggttcgtgaa
    32881 gatctgaccg cgctggattt tgtcaccatc gacagtgcca gcacagaaga tatggatgac
    32941 gcccttttcg ctaaggcgtt gccggatgac aaacttcagc tgattgtggc gattgccgat
    33001 ccaaccgcgt ggattgctga aggcagcaag ctggacaaag ccgcgaaaat tcgcgcattc
    33061 accaactatc tgcctggctt caacatccct atgcttcctc gcgagctttc tgacgatctc
    33121 tgctcactgc gcgccaatga agtccgcccg gtactggcat gccgcatgac gctctccgct
    33181 gatggcacca ttgaagataa tatcgaattc tttgccgcca ccatcgaatc caaagcgaag
    33241 ctggtgtatg accaggtttc tgactggctg gaaaataccg gtgactggca gcctgaaagt
    33301 gaagcaattg ccgaacaagt ccgtttgcta gcgcaaattt gccaacgccg cggcgagtgg
    33361 cgtcataacc acgcactggt gtttaaagat cgcccggatt accgctttat tctcggtgaa
    33421 aaaggtgaag tgctggatat cgtcgccgag cctcgtcgca ttgccaaccg tatcgtcgaa
    33481 gaagcgatga ttgccgctaa catttgtgcg gcccgcgttc tgcgcgataa gctcggtttt
    33541 ggcatctata acgtgcatat gggctttgat ccggcgaatg ccgacgcgct ggcagcgttg
    33601 ctgaaaacgc acggtctgca tgtcgatgcc gaagaagtgc tcacgctgga cggtttctgc
    33661 aaactgcgtc gtgaactgga tgcgcaacca actggtttcc tcgacagccg cattcgtcgc
    33721 ttccagtcat ttgctgaaat tagcactgaa cccggtcctc actttggcct cggtctggaa
    33781 gcatacgcca cctggacttc gccgatccgt aaatatggcg acatgatcaa ccaccgtctg
    33841 ctgaaagcgg ttatcaaagg cgaaactgcg acgcgtccgc aggatgaaat cactgtccaa
    33901 atggccgaac gtcgccgtct caaccggatg gcagaacgtg atgttggcga ctggttatac
    33961 gcacgcttcc tgaaagataa agccgggacc ggcacccgtt tcgcggcgga aattgtcgat
    34021 atcagccgtg gcggcatgcg tgtgcgtttg gttgataacg gcgctatcgc ctttattccg
    34081 gcacctttct tacacgctgt gcgcgatgaa ctggtttgca gccaggaaaa cggcactgta
    34141 caaattaaag gtgaaacggt ttacaaagta actgacgtta ttgacgtcac cattgccgaa
    34201 gtccgcatgg aaacccgcgg cattattgcg cgcccggtcg cgtaatctcc tttcacggcc
    34261 cattcctcat ggatgggccg tttatttccc cgctctacct tcgttatatc caggcagatt
    34321 ttttaactga ctttcgtttg aaaactggcg tttttccaaa actggattac aaataaatat
    34381 ataaaaaaac aatgcgttta gccgccgtta atttcatgta agtcatgttg ttccttttat
    34441 ttaaccgcgg actccgcagt taaccggagg atatgcatca tgaaaaccgt tagggagtcc
    34501 acaacgttgt acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc
    34561 gatgttttgc gcttttctac caccgaaacc acagaacctg atcgtacttt gcagttatct
    34621 gccgaacagg ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt
    34681 ctgaccgtcg atgagagcga tctttctgtg catctggtag gacgaaaaat caataaacgg
    34741 gaatgggctg gcaacgcgtc tgcatggcat gacacaccgg cagttgctcg tgatttatca
    34801 cacgggcttt cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc
    34861 gacagccggg ggaatatcca acgcttcaat cggttatgtg aagattacac tgggttgaaa
    34921 gaacacgacg tcattggcca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg
    34981 gcatccaggc gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg
    35041 tggataccaa cacgtaaagg ccagcggctg tttctgtttc gcaataaaat aaatttgtcc
    35101 acagcggcag tggcaaaaac gagatttttt taatctgttc cggtaccgac attaccgaag
    35161 agcgccgcgc tcaggagcga ctgcgtattc tggcaaatac cgacagtatc accggactgc
    35221 cgaatcgtaa cgcaatgcag gagttaatcg atcacgctat taatcaggca gataacaata
    35281 aagttggggt tgtgtatctt gatttggata attttaaaaa ggtcaacgac gcctatgggc
    35341 atttgtttgg tgaccagtta ttacgcgacg tgtcattggc tattttaagc tgtctcgaac
    35401 atgaccaggt gttggcgcgt ccaggtggcg atgagtttct ggtactggca tccaatacct
    35461 cacaaagcgc gctggaagca atggcatcac gaattttgac ccgcttacgc ctcccctttc
    35521 gtattggttt aattgaagtt tataccagct gttcagtagg tatttcgctc tctcccgaac
    35581 atggttcgga cagcgcgact attattcgtc acgccgacac agcaatgtac acagcgaagg
    35641 aaggcggacg aggacaattt tgcgttttta ctccagaaat gaatcagcgg gtatttgaat
    35701 atctctggct ggataccaac ttgcgtaaag cactggaaaa cgatcagttg gttattcact
    35761 atcaaccgaa aatcacctgg cgtggcgaag tgcgcagtct ggaagcacta gtacgttggc
    35821 agtcacctga acgtgggttg attccaccgt tggacttcat ttcctacgcc gaagagtcag
    35881 ggctaattgt gcctttaggc cgttgggtga ttctcgatgt cgtacgccag gtggcaaagt
    35941 ggcgggataa aggcataaac ctgcgagtgg cggtaaatat ttctgcacgt cagctcgccg
    36001 atcaaaccat tttcaccgcc ctgaaacagg ttctccagga actcaatttt gaatactgcc
    36061 ctatagatgt tgaactgaca gagagttgtc tgattgagaa tgatgaactg gcactgtctg
    36121 ttattcaaca atttagccaa ctaggtgcgc aagtgcatct ggatgatttt ggtaccggct
    36181 actcttcact ttcgcaactg gcgcgctttc cgatcgatgc catcaaactt gaccaggttt
    36241 ttgttcgaga tattcacaaa caacctgtct cgcagtcact ggtccgggcg atcgtcgctg
    36301 tggcccaggc attgaatctt caggtgatcg ccgaaggtgt agagagtgca aaggaagatg
    36361 cttttttaac caagaacggg atcaatgagc ggcaaggatt tttgtttgcc aaaccgatgc
    36421 ccgccgtcgc cttcgaacgc tggtataaac gctatctgaa gcgcgcataa gtagatgaat
    36481 atatatactt agcgctatat cccgtcatta tatatcgtat tcctggatga taattagccg
    36541 ttgctgttaa acacttctct tttagcggca atttttgcat tattcatttt ccacttttgc
    36601 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
    36661 tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact
    36721 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga aggcaccgtg
    36781 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
    36841 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
    36901 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
    36961 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
    37021 cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg
    37081 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
    37141 gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggggag
    37201 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
    37261 gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
    37321 ggcccgttga cacgccagtg catccaacag gtgcatttca gcaaagcatt tattggtatt
    37381 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgtac cgatgtggtc
    37441 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
    37501 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
    37561 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt
    37621 gacatttaac catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta
    37681 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacacgct taagatataa
    37741 aattattatc agcgatataa caggaagtca ttatcacctg cgtgatataa ccctgcgcgc
    37801 gagcagattt cacggaataa tttcaccagg cttattctta gctattatag ttatagagag
    37861 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    37921 gaccgcggct gttctggcaa ttactttggc aatgtctctg agtgcctgtt ctaactggtc
    37981 taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    38041 gaccgatggc agtacgttgg gtacattagg tggtgcagct gtcggtggtg ttattggtca
    38101 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt
    38161 gattctgttc agtacgaaat aactgaataa tacgcacagt aaaatattcg aaagccacgg
    38221 atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc ctttcgcttc
    38281 aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc catctttaac
    38341 tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca gttcggcatc
    38401 atcgagatcg acaccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    38461 ctgaatacgc accacaccgt cgcctttagg gcgtacaggg gccgctttgg gctcatcaat
    38521 acgtccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    38581 tgtaaagagg cgttgatcgc tttcagcgtc tgcgctggat ctaccgattg cgttaccggg
    38641 cgaccaatca ccatataatc aacaccagcc gacaacgcct gttctggcgt cataatgcgg
    38701 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gcgtaaccag tttgaactcc
    38761 tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg
    38821 ccacattttt gcgtcagtgc cgccagacgt tctgcataat ctgcaggtga cagtgtcatg
    38881 ccaagatcga ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc
    38941 ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    39001 ccagaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    39061 gctgcagtgt taggaatatc gtggaatttc aggtcaagaa agatatcaaa accacgctgt
    39121 tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttgcc gaccttcaga
    39181 cgacaatcgc gtgggtcgat cttgtcgaca aaggacagcg cgtcatcacg attatgataa
    39241 tcaagggcaa caaccacagg agaattcgta acagcgcggg aagaagatga agcagttaac
    39301 gtcatgacca gaccttcttg atgatgggca ccggaagtgg cgcgaaacag gtaaacggcg
    39361 agcattctac ccgtggaaat aaaaaattaa cagttgcgat ttttcccctg gcaggctttt
    39421 tctgctccgt cgttgccgga gcacaatgga acatcaataa ctagtatgtt gtaactaaag
    39481 tcggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat ggttgaccag
    39541 gcccgacaag acggacaatg ccagtagagg gtgtatgcgg taaaaccaca tttctggcag
    39601 cgataacgag gcttactacg taccttttcg ccaaccatgt cacgcagcac catcaggctc
    39661 tctttggcac gcccttcttc cgcttcattt aagtggtaat ccattaactt atggaacaca
    39721 cgcatggtcg gatgacgctg aagctggcgc gtaatataga cctgtgcggc ctcactaccg
    39781 tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc
    39841 accgcgcgtt gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    39901 tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gggatatcac gcgttgcaaa
    39961 ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat
    40021 acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg
    40081 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    40141 cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac
    40201 tcgctggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg gaagtcagtt
    40261 tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa
    40321 tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagtga ggcgctttcc
    40381 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    40441 ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg
    40501 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    40561 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    40621 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    40681 ttcgccgctg acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc
    40741 agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacagg
    40801 ccacaaatca accaaccgat agcaaccccc gcagcaaaca ataccgccag caatgtggaa
    40861 atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca
    40921 cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc
    40981 acattacttc ccgttatgtg gttaaagcga ataaattatg gtcaacttac cgcaatagcc
    41041 ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gttgatgatt
    41101 taagggctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gcgctatttc
    41161 gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa gccacgttgc
    41221 caccgccacc agcgcccacg aaatcaacgt agctactacc agatcgcgtg gccaatgcat
    41281 cccgagcagc aggcggcttc ccatgactcc cgttgcccag accagcaaga tagcaatggt
    41341 taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac tggcagcaaa
    41401 catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg aacgcaaata
    41461 ttgtgggata tttttctctt cagccaactg ttctttcact agatttccgc gttctgctcg
    41521 ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccagcc agataacaaa
    41581 aggtcgtggt tcctggactt tgtctttgat ccaggattta acgccttgtc ccacaatgat
    41641 tacggccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca
    41701 gagaaaccag ccgaataaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc
    41761 agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccagcc
    41821 agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg cggtacgtct
    41881 ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaacgcc
    41941 agttccagga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta
    42001 ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa
    42061 tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct
    42121 gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga
    42181 tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc
    42241 cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga cgcaaattgc
    42301 cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct
    42361 gaataaaatc cgcgcttacg cactgcagga tcaaggttac gataccgtag aggctaacca
    42421 tcagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact
    42481 ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac
    42541 cgaagcaggg attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa
    42601 cgaacattat ctcaatacca aagccgagaa aatgggtcat ttgctgaaca aataaccctc
    42661 tcgcattgtg gaattaattt gcttgccgga agcaaaataa ccggcaagca aatatttgtt
    42721 acttcagcat attacgaatg acataatgca aaatgccgtc gttctggtag taggtcaact
    42781 ccgtcgcggt gtcgatacga caacggcagg gtacgacctc ctggctacca tccgcgcgcg
    42841 taagcgtcac cggaaccgtc gcaccgggtt gtagattttg cagatcgcca atatcaatct
    42901 tctcttcccc ggttagtcct aacgttttac gcgttacgcc ttgcggaaat tccagcggca
    42961 ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca
    43021 cacgaattcc aagcagacga ggaccttttg ccgcccagtc acggcttgag cctgaaccat
    43081 actctttccc ggcaatcacc gccagcggcg tttgttcctg cttatagcgc atcgcagcat
    43141 cataaataga gactacgtcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag
    43201 gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt
    43261 taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtagat
    43321 atcgacccgc tgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga
    43381 ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccact ggtgctggtg
    43441 ttgcctgcat ttcatcaaag aaaggcgata agcgaatata ggttgagtcc tcctgccaac
    43501 cgtaggtatc ggatcgtgtg acgttaattt ccttccactc tgctgtgcct tcaaaaactt
    43561 ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt
    43621 gtgccgatgg ccagatatct ttcagataaa ccggatcgcc tttacgatca tgaccaatag
    43681 gctcagaagc caggttgata ttcatatttc ccgccagcgc ataggcaacc accagcggcg
    43741 gcgaggccag ccagttggtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc
    43801 cggacagcac tgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg
    43861 gcagcggccc agagttacca atacaggtag tacaaccgta tcccacaagg ttaaacccca
    43921 gttcgtcgag aaacggtgtc agtttcgctt ttgccagata gtcagaaaca actttcgagc
    43981 ctggtgccag cgacgctttg acccacggtt gccgcttgag gccaagaatt acggcttttt
    44041 tcgccagcaa gcctgcagcc atcagcacac ttgggttaga ggtgttggtg cacgaggtta
    44101 tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc ataacataat
    44161 cgaccggctg gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaata
    44221 cttttggtac atcgggcagt gcaacgcgat cctgtgggcg ttttggccct gccaggctcg
    44281 cttcaacgtc attcatatcc agttctaacg tactggtaaa aatcggttca tcgcccgggt
    44341 tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcaacctga tcttcgctgc
    44401 gcccgcttaa acgcatgtaa tcgagggtta cagcatcgat tgggaagaag ccacaggtgg
    44461 caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccagcggt agtgaatcca
    44521 gaccatcacc ataaaattcg acgaatttct ccaccacgcc atgtttgcgc agcatttggg
    44581 taacagtgag aaccaggtct gtggcggtaa taccttcacg taatttaccg gtaagtttaa
    44641 agcccactac gtccgggata agcatggaaa ccggctggcc taacattgcg gcttctgctt
    44701 cgatcccacc cacgccccac cccagcacgc caaggccgtt gatcatagtg gtgtgcgagt
    44761 cagtaccaac gagtgtatcc ggataagcaa tccattcacc gtcctgcaat tcactccaca
    44821 ctgctttgcc gagatattcg aggttaacct gatggcaaat gcctgtgcct ggcggcacga
    44881 cgctaaaccg actgaacgct tgctttcccc atttcaggaa cacataacgt tcgtggttgc
    44941 gctccatttc caggcgtacg ttttcttcaa atgcctcatc atcaccaaaa cgatcgacgg
    45001 tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg gttaaccttt gcagtatcgc
    45061 cgccgaggcg tttaaccgct tcgcgcattg ccgccagatc aacaacggca ggtacgccgg
    45121 taaagtcctg catcaacacc cttgccgggc ggtaggcaat ttcacggtca gcatgggcat
    45181 ttttcagcca tcctgccagc gcgtggatat cctcttcggt aaccgagtta ccatcctgcc
    45241 agcgcagcag gttttcgagc aaaactttga gtgacttggg tagacgggtg atatcgccca
    45301 gtgatttagc agcaagcggc aggctgtagt agtggtaagt tttatctttg gcctgcaacg
    45361 tgtccttact ggcttctcgt agggttgacg acatagctcc tccttaatga cagggttgcg
    45421 aagccctgat tctcttcagg gtgaatatta aagataacac aaacatcgcg taacgatttg
    45481 ataacaaccc aaattgataa aagaggagaa accttgtggc aaagcaaatg acaaccccgc
    45541 cgcagcgggg tcaaggatca gtgagtaatg atccagagga gttcggccca gaagagcatc
    45601 gacagagaaa acgcgcccat ccaggaccaa tatttcatat ttgtgttgat cataggctac
    45661 acccttaacg gttattattt tgtgttgaga taactcaagc tgttattgct gcggtgtatt
    45721 aaaagccctt taattcatta aaaatgggta ataagaatga tgataattat tgtttcggct
    45781 gatcatcttc agcaaacagt tctataaagg cttgctgctg cgatgttagt tgccagtcgt
    45841 ctggaataac gacggaacat tcccgtttcg tttctgtttc attgtcacta tgctggcata
    45901 atggtgacga ctccagttgc tcctgaccga ccatagttat ccccacactt tccaaatcag
    45961 cagtgcgaca accacccaga aaagtgcaga accaagaaaa accgccagcc aggctttacg
    46021 ttttaattca ggatcgcggc ggggttcctg atttccagaa ggcattgcta acctcatata
    46081 atcgccatca cttatcagca agacgttgaa cgattggtgt aattaatcat acgatgagac
    46141 aaatcctaaa gaaactttag t
//
LOCUS       DAQSWQ010000022        45986 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.022, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000022 DAQSWQ010000000
VERSION     DAQSWQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.022"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(368..712)
                     /gene="tdcR"
                     /locus_tag="R8G18_001280"
     CDS             complement(368..712)
                     /gene="tdcR"
                     /locus_tag="R8G18_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HEE9795036.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            901..1839
                     /gene="tdcA"
                     /locus_tag="R8G18_001281"
     CDS             901..1839
                     /gene="tdcA"
                     /locus_tag="R8G18_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HEE9795037.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            1938..2927
                     /gene="tdcB"
                     /locus_tag="R8G18_001282"
     CDS             1938..2927
                     /gene="tdcB"
                     /locus_tag="R8G18_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HEE9795038.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2949..4280
                     /gene="tdcC"
                     /locus_tag="R8G18_001283"
     CDS             2949..4280
                     /gene="tdcC"
                     /locus_tag="R8G18_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HEE9795039.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            4306..5514
                     /gene="tdcD"
                     /locus_tag="R8G18_001284"
     CDS             4306..5514
                     /gene="tdcD"
                     /locus_tag="R8G18_001284"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HEE9795040.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            5548..7842
                     /gene="tdcE"
                     /locus_tag="R8G18_001285"
     CDS             5548..7842
                     /gene="tdcE"
                     /locus_tag="R8G18_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HEE9795041.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7856..8245
                     /locus_tag="R8G18_001286"
     CDS             7856..8245
                     /locus_tag="R8G18_001286"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HEE9795042.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            8317..9681
                     /gene="tdcG"
                     /locus_tag="R8G18_001287"
     CDS             8317..9681
                     /gene="tdcG"
                     /locus_tag="R8G18_001287"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEE9795043.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALCSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9956..11287
                     /locus_tag="R8G18_001288"
     CDS             9956..11287
                     /locus_tag="R8G18_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HEE9795044.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            11315..12625
                     /locus_tag="R8G18_001289"
     CDS             11315..12625
                     /locus_tag="R8G18_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HEE9795045.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(12759..12923)
                     /locus_tag="R8G18_001290"
     CDS             complement(12759..12923)
                     /locus_tag="R8G18_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795046.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12946..13647)
                     /locus_tag="R8G18_001291"
     CDS             complement(12946..13647)
                     /locus_tag="R8G18_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEE9795047.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            13769..13951
                     /locus_tag="R8G18_001292"
                     /pseudo
     CDS             13769..13951
                     /locus_tag="R8G18_001292"
                     /inference="COORDINATES: protein motif:HMM:NF012354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            13952..14644
                     /locus_tag="R8G18_001293"
                     /pseudo
     CDS             13952..14644
                     /locus_tag="R8G18_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004994521.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(14695..15051)
                     /locus_tag="R8G18_001294"
     CDS             complement(14695..15051)
                     /locus_tag="R8G18_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEE9795048.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(15293..15658)
                     /locus_tag="R8G18_001295"
     CDS             complement(15293..15658)
                     /locus_tag="R8G18_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEE9795049.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPSENRFGPDPKLEP"
     gene            complement(15923..16936)
                     /gene="yqjG"
                     /locus_tag="R8G18_001296"
     CDS             complement(15923..16936)
                     /gene="yqjG"
                     /locus_tag="R8G18_001296"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HEE9795050.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            complement(17006..17398)
                     /locus_tag="R8G18_001297"
     CDS             complement(17006..17398)
                     /locus_tag="R8G18_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEE9795051.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(17584..17883)
                     /locus_tag="R8G18_001298"
     CDS             complement(17584..17883)
                     /locus_tag="R8G18_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HEE9795052.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(17873..18277)
                     /locus_tag="R8G18_001299"
     CDS             complement(17873..18277)
                     /locus_tag="R8G18_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEE9795053.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(18280..18585)
                     /locus_tag="R8G18_001300"
     CDS             complement(18280..18585)
                     /locus_tag="R8G18_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEE9795054.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(18623..18991)
                     /locus_tag="R8G18_001301"
     CDS             complement(18623..18991)
                     /locus_tag="R8G18_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HEE9795055.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(19138..19521)
                     /gene="mzrA"
                     /locus_tag="R8G18_001302"
     CDS             complement(19138..19521)
                     /gene="mzrA"
                     /locus_tag="R8G18_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HEE9795056.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(19525..20187)
                     /locus_tag="R8G18_001303"
     CDS             complement(19525..20187)
                     /locus_tag="R8G18_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEE9795057.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(20532..21308)
                     /gene="exuR"
                     /locus_tag="R8G18_001304"
     CDS             complement(20532..21308)
                     /gene="exuR"
                     /locus_tag="R8G18_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HEE9795058.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            21604..22320
                     /locus_tag="R8G18_001305"
     CDS             21604..22320
                     /locus_tag="R8G18_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEE9795059.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            22350..22850
                     /locus_tag="R8G18_001306"
     CDS             22350..22850
                     /locus_tag="R8G18_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9795060.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            22942..25626
                     /locus_tag="R8G18_001307"
     CDS             22942..25626
                     /locus_tag="R8G18_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HEE9795061.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            25623..26711
                     /locus_tag="R8G18_001308"
     CDS             25623..26711
                     /locus_tag="R8G18_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HEE9795062.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(26794..28091)
                     /gene="exuT"
                     /locus_tag="R8G18_001309"
                     /pseudo
     CDS             complement(26794..28091)
                     /gene="exuT"
                     /locus_tag="R8G18_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            28574..29986
                     /gene="uxaC"
                     /locus_tag="R8G18_001310"
     CDS             28574..29986
                     /gene="uxaC"
                     /locus_tag="R8G18_001310"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEE9795063.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            30001..31488
                     /gene="uxaA"
                     /locus_tag="R8G18_001311"
     CDS             30001..31488
                     /gene="uxaA"
                     /locus_tag="R8G18_001311"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEE9795064.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            31571..32122
                     /locus_tag="R8G18_001312"
     CDS             31571..32122
                     /locus_tag="R8G18_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HEE9795065.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(32127..33371)
                     /gene="sstT"
                     /locus_tag="R8G18_001313"
     CDS             complement(32127..33371)
                     /gene="sstT"
                     /locus_tag="R8G18_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEE9795066.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(33987..34952)
                     /gene="alx"
                     /locus_tag="R8G18_001314"
     CDS             complement(33987..34952)
                     /gene="alx"
                     /locus_tag="R8G18_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HEE9795067.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(35235..36221)
                     /locus_tag="R8G18_001315"
     CDS             complement(35235..36221)
                     /locus_tag="R8G18_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEE9795068.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(36300..36983)
                     /locus_tag="R8G18_001316"
     CDS             complement(36300..36983)
                     /locus_tag="R8G18_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HEE9795069.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(37060..37563)
                     /locus_tag="R8G18_001317"
     CDS             complement(37060..37563)
                     /locus_tag="R8G18_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEE9795070.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            37648..38784
                     /gene="rlmG"
                     /locus_tag="R8G18_001318"
     CDS             37648..38784
                     /gene="rlmG"
                     /locus_tag="R8G18_001318"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HEE9795071.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            39069..39383
                     /gene="higB"
                     /locus_tag="R8G18_001319"
     CDS             39069..39383
                     /gene="higB"
                     /locus_tag="R8G18_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HEE9795072.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            39380..39796
                     /gene="higA"
                     /locus_tag="R8G18_001320"
     CDS             39380..39796
                     /gene="higA"
                     /locus_tag="R8G18_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HEE9795073.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(39841..41859)
                     /gene="fadH"
                     /locus_tag="R8G18_001321"
     CDS             complement(39841..41859)
                     /gene="fadH"
                     /locus_tag="R8G18_001321"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEE9795074.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(42085..44436)
                     /gene="ygjK"
                     /locus_tag="R8G18_001322"
     CDS             complement(42085..44436)
                     /gene="ygjK"
                     /locus_tag="R8G18_001322"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEE9795075.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(44453..45232)
                     /locus_tag="R8G18_001323"
     CDS             complement(44453..45232)
                     /locus_tag="R8G18_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEE9795076.1"
                     /translation="MQADHDGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQ
                     DSSLDDLQWWGDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAW
                     LHFGKYDRYDDKYTYPAMMNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAA
                     RAWHHTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQ
                     FGIWYEYGGGRFATAFDSRFYMKNASHDPSDQIFLMQYFYW"
     gene            complement(45263..45530)
                     /locus_tag="R8G18_001324"
                     /pseudo
     CDS             complement(45263..45530)
                     /locus_tag="R8G18_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(45664..45978)
                     /locus_tag="R8G18_001325"
     CDS             complement(45664..45978)
                     /locus_tag="R8G18_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEE9795077.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
BASE COUNT        11507 a        11258 c        11790 g        11431 t
ORIGIN      
        1 caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt
       61 gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct
      121 gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac
      181 tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac
      241 gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga
      301 tcagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga
      361 gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt
      421 tgacgttaat caattgttcg agatgatcaa tacctgtatt cgaaatttta aaatattgat
      481 aaaataaatg tttgttctcc aacaaatagc gtgaaaaaat gtgacgtgcc gcagtattta
      541 tcgctgaaaa tggtttattt ataataaaat ttgagaactt gctcattaaa tttaactcaa
      601 attttgcctg gtaattatcc ggtaattgct tgaaatatgg tctcagcccc ttttttgtat
      661 taaccacata acgaatgatg ttatcgccat aaaatatggt tatccccgtc attttttttg
      721 acaaaaatca gggtttatgc tgatttttat gctttaactt gttgatattt aaaggtgttt
      781 aattgtaatg acgataccct ggaaagtatt gaaagttaat ttgtgagtgg tcgcacatat
      841 cctgttcatt tcattttgat acacttcatg ccgtcaatga ggtaattaac gtaggtcgtt
      901 atgagcacta ttcttcttcc gaaaacgcag cacctggtag tctttcagga agtcattaga
      961 agtggctcta tcggctcggc tgcaaaagaa ttagggttaa ctcaaccggc cgtcagtaaa
     1021 atcattaacg atattgaaga ttattttggt gtggaattag tggtgcggaa aaataccggt
     1081 gtaacattaa cacctgccgg tcaattgtta ctctcccgtt ccgaatccat tacccgtgaa
     1141 atgaaaaata tggttaatga gataagcggt atgtcttctg aggcggtggt ggaagtctca
     1201 tttggttttc cttcattgat tggttttact tttatgtcag ggatgatcaa caagttcaaa
     1261 gaggtgttcc cgaaagcgca ggtttctatg tatgaagcgc aactgtcttc gttcttaccg
     1321 gcaatccgcg acggtcgact ggattttgcg attggtacgt taagtgcaga aatgaagctt
     1381 caggatttac atgttgagcc gctgttcgag tccgagtttg tgctggtagc cagtaagtcc
     1441 cgaacatgca ccggcaccac cacgctggag tcgttgaaga acgaacagtg ggtgttgcca
     1501 caaactaata tggggtacta cagcgaactg cttactacgt tacaaagaaa tggcatcagt
     1561 attgaaaaca tcgttaaaac cgactcagtc gtgacaattt ataatcttgt tctcaatgct
     1621 gatttcttaa ctgtaattcc ttgtgatatg acgtcacctt ttggttctaa tcaatttatt
     1681 actattccgg ttgaagaaac attacctgtg gcacaatatg ccgcggtatg gtcgaaaaat
     1741 tatcgtatta aaaaagcagc atcggttttg gtggaattag ccaaagagta ttcatcttat
     1801 aatgggtgta gacgaaggca attaatagaa gttggttagt tatttgtttt tatttaaaca
     1861 taaataatcc acctgtctgt tttgcaggtg tcggttacgg ttacctacat atttaattca
     1921 ggcgaagagg ttttataatg catattacat acgatctgcc gattgctatt gatgatatta
     1981 ttgaagcgaa acaacgactg gctgggcgaa tttataaaac aggcatgcct cgctccaact
     2041 attttagtga acgttgcaaa ggtgaaatat tcctgaagtt tgaaaatatg cagcgtacgg
     2101 gttcatttaa aattcgtggc gcatttaata aattaagttc actgaccgat gcggaaaaac
     2161 gcaaaggcgt ggtggcctgt tctgcgggca accatgcgca aggggtttcg ctctcctgcg
     2221 cgatgctggg tatcgacggt aaagtggtga tgccaaaagg tgcgccaaaa tccaaagtag
     2281 cggcaacgtg cgactactcc gcagaagtcg ttctgcatgg tgataacttc aacgacacta
     2341 tcgctaaagt gagcgaaatt gtcgaaatgg aaggccgtat ttttatccca ccttacgatg
     2401 atccgaaagt gattgctggc cagggaacga ttggtctgga aattatggaa gatctctatg
     2461 atgtcgataa cgtgattgtg ccaattggtg gtggcggttt aattgctggt attgcggtgg
     2521 caattaaatc tattaacccg accattcgtg ttattggcgt acagtctgaa aacgttcacg
     2581 gcatggcggc ttctttccac tccggagaaa taaccacgca ccgaactacc ggcaccctgg
     2641 cggatggttg tgatgtctcc cgcccgggta atttaactta cgaaatcgtt cgtgaattag
     2701 tcgatgacat cgtgctggtc agcgaagacg aaatcagaaa cagtatgatt gccttaattc
     2761 agcgcaataa agtcgtcacc gaaggcgcag gcgctctggc atgtgctgca ttattaagcg
     2821 gtaaattaga ccaatatatt caaaacagaa aaaccgtcag tattatttcc ggcggcaata
     2881 tcgatctttc tcgcgtctct caaatcaccg gtttcgttga cgcttaatta attcgttgag
     2941 gataggatat gagtacttca gatagcattg tatccagcca gacaaaacaa tcgtcctggc
     3001 gtaaatcaga taccacatgg acgttaggct tgtttggtac ggcaatcggc gccggggtgc
     3061 tgttcttccc tatccgcgca ggttttggcg gactgatccc gattcttctg atgttggtat
     3121 tggcataccc catcgcattt tattgccacc gggcgctggc gcgtctatgt ctttctggct
     3181 ctaacccttc cggcaacatt acggaaacgg tggaagagca ttttggtaaa actggcggcg
     3241 tggttatcac gttcctgtac ttcttcgcga tttgcccact gctgtggatt tatggcgtta
     3301 ctattaccaa tacctttatg acgttctggg aaaaccagct cggctttgca ccgctgaatc
     3361 gcggctttgt ggcgctgttc ctgctgctgc tgatggcttt cgtcatctgg tttggtaagg
     3421 atctgatggt taaagtgatg agctacctgg tatggccgtt tatcgccagc ctggtgctga
     3481 tttctctgtc gctgatccct tactggaact ctgcagttat cgaccaggtt gacctcggtt
     3541 cgctgtcgtt aaccggtcat gacggtatcc tgatcaccgt ctggctgggg atttccatca
     3601 tggttttctc ctttaatttc tcgccaatcg tctcttcctt cgtggtttct aaacgtgaag
     3661 agtatgagaa agacttcggt cgcgacttca ccgaacgtaa atgttcccaa atcatttctc
     3721 gtgccagcat gctgatggtt gcagtggtga tgttctttgc ctttagctgc ctatttacgc
     3781 tgtctccggc caacatggcg gaagccaaag cgcagaatat tccagtgctt tcttatctgg
     3841 ctaaccactt tgcgtccatg accggtacca aaacaacgtt cgcgattaca ctggaatatg
     3901 cggcttccat catcgcactc gtggctatct tcaaatcttt cttcggtcac tatctgggaa
     3961 cgctggaagg tctgaatggc ctggtcctga agtttggtta taaaggcgac aaaactaaag
     4021 tgtcgctggg taaactgaac actatcagca tgatcttcat catgggctcc acctgggttg
     4081 ttgcctacgc caacccgaac atcctcgacc tgattgaagc catgggcgca ccgattatcg
     4141 catccctgct gtgcctgttg ccgatgtatg ccatccgtaa agcgccgtct ctggcgaaat
     4201 accgtggtcg tctggataac gtgtttgtta ccgtgattgg tctgctgacc atcctgaaca
     4261 tcgtatacaa actgttttaa tccgtaactc aggatgagaa aagagatgaa tgaatttccg
     4321 gttgttttgg ttattaactg tggttcgtct tcgattaagt tttccgtgct cgatgccagc
     4381 gactgtgaag tattaatgtc aggtattgcc gacggtatta actcggaaaa tgcattctta
     4441 tccgtaaatg ggggagagcc agcaccgctg gctcaccaca gctacgaagg tgcattgaag
     4501 gcaattgcat ttgaactgga aaaacggaat ttaaatgaca gtgtggcctt aattggccac
     4561 cgcatcgctc acggcggcag tatttttacc gagtccgcca ttattaccga tgaagtcatt
     4621 gataatatcc gtcgcgtttc tccactggca cccctgcata attacgccaa tttaagtggt
     4681 attgaatcgg cgcagcaatt atttccgggc gtaactcagg tggcggtatt tgataccagt
     4741 ttccaccaga cgatggctcc ggaagcttat ttatacggcc tgccgtggaa atattatgaa
     4801 gagttaggtg tacgccgtta tggtttccac ggcacgtcgc accgctatgt ttcccagcgc
     4861 gcacattctc tgctgaatct ggcggaagat gactccggcc tggttgtggc gcatcttggc
     4921 aatggcgcgt caatctgcgc ggttcgcaac ggtcagagtg ttgatacttc aatgggaatg
     4981 acgccgctgg aaggcttgat gatgggaacc cgcagtggcg atgtcgactt tggtgcgatg
     5041 tcctgggtcg ccagccaaac caaccagagc ctgggtgacc tggaacgcgt agtgaataaa
     5101 gagtcgggat tattaggtat ttccggtctt tcttcggatt tacgtgttct ggaaaaagcc
     5161 tggcatgaag gtcacgaacg cgcgcaactg gcaattaaaa cctttgttca ccgaattgcc
     5221 cgtcatattg ccggacacgc agcttcatta cgtcgcctgg atggaattat attcaccggc
     5281 ggaataggag agaattcaag tttaattcgt cgtctggtca tggaacattt ggctgtatta
     5341 ggcgtagtga ttgatacaga aatgaataat cgctctaact cttttggaga gcgaattgtt
     5401 tccagtgaaa atgcgcgtgt catttgtgcc gttattccga ctaacgaaga aaaaatgatt
     5461 gctttggatg ccattcattt aggcaaagtt aacgcgcccg cagaatttgc ataatttagt
     5521 tgaagtattg tagagagatt atttttcatg aagatagata ttgataccag cgataagctg
     5581 tacgccgacg catggcttgg ctttaaaggt acggactgga aaaacgaaat taatgtccgc
     5641 gattttattc aacataacta tacaccgtat gaaggcgatg aatctttcct cgccgaagcg
     5701 acgcctgcca ccacggaatt gtgggaaaaa gtaatggaag gcatccgtat cgaaaacgca
     5761 acccacgcgc cggttgattt cgataccaat attgccacca caattaccgc tcatgatgcg
     5821 ggatatatta accagccgct ggaaaaaatt gttggtctgc aaacggatgc gccattaaag
     5881 cgcgcgctgc atccgttcgg tggcattaat atgattaaaa gttcattcca cgcctatggc
     5941 cgtgaaatgg acagtgaatt tgaatatctg tttaccgatc tgcgtaaaac ccataaccag
     6001 ggcgtatttg atgtttactc accggatatg ctgcgctgcc gtaaatctgg cgtgctgacc
     6061 ggtttaccag atggctatgg ccgtgggcgc attatcggtg actatcgccg cgtagcgctg
     6121 tatggcatca gttatctggt gcgtgaacgc gaactgcaat ttgccgatct ccagtcccgt
     6181 ctggaaaaag gcgaagatct ggaagccacc atccgtctgc gtgaggagct ggcagagcat
     6241 cgtcatgcgc tgttgcagat tcaggaaatg gcggcgaaat atggctttga tatctctcgc
     6301 ccggcgcaga atgcgcagga agcggtgcag tggctctact tcgcttatct ggcggcagtg
     6361 aaatcacaga acggcggcgc gatgtcgctg gggcgcacgg cgtcgttcct cgatatctac
     6421 attgagcgcg actttaaagc aggcgtactc aatgagcagc aggcacagga actgatcgat
     6481 cacttcatta tgaagatccg tatggtgcgc ttcttgcgta cgccggaatt tgattcgctg
     6541 ttctccggcg acccaatctg ggcgacggaa gtgatcggcg ggatggggct ggacggtcgt
     6601 acgctggtga ccaaaaactc cttccgctat ttgcacaccc tgcacactat ggggccggca
     6661 ccggaaccta acctgaccat tctttggtcg gaagaattac cgattgcctt caaaaaatat
     6721 gccgcgcagg tgtcgatcgt cacctcttcc ttgcagtatg aaaacgacga tctgatgcgt
     6781 actgacttca acagcgacga ttacgcaatt gcttgttgcg tcagcccaat ggtgattggt
     6841 aagcaaatgc agttctttgg tgcacgcgct aacctggcga aaacactgct gtacgcaatt
     6901 aacggcgggg tggacgagaa gctgaagatt caggtcgggc cgaaaacagc accgctgatg
     6961 gacgacgtgc tggattacga caaagtgatg gacagcctcg atcacttcat ggactggctg
     7021 gcggtgcagt acatcagcgc gctgaatatc attcactaca tgcacgacaa gtacagctac
     7081 gaagcttcgc tgatggcgct gcacgatcgt gatgtctatc gcactatggc atgcggcatc
     7141 gcgggcctgt cggtggcgac ggactccctg tctgccatca aatatgcccg cgtgaaacca
     7201 atccgtgacg aaaacggcct ggcggtggac tttgaaatcg acggtgaata tccgcagtac
     7261 ggcaacaacg acgagcgcgt agacagcatt gcctgcgacc tggttgaacg ctttatgaag
     7321 aaaattaaag cgctgccaac ctatcgcaac gccgtcccta cccagtcgat tctgactatc
     7381 acttctaacg tggtgtacgg ccagaaaacc ggtaatacgc cggacggtcg tcgcgccgga
     7441 acaccgttcg cgccgggcgc taacccgatg catggtcgtg accgcaaagg tgccgtggcc
     7501 tcattgacgt cggtggcgaa actgccgttc acctacgcca aagatgggat ctcgtacacc
     7561 ttctcaatcg ttcctgcggc gctgggcaaa gaagatccag tacgtaaaac caaccttgtc
     7621 ggcctgctgg atgggtattt ccaccacgaa gcggatgtcg aaggcggtca acacctcaac
     7681 gtcaacgtaa tgaatcggga aatgctgctg gatgccatcg agcacccgga aaaatatcct
     7741 aacctgacaa tccgtgtctc tggctacgcc gtgcgcttca acgcactgac ccgtgaacag
     7801 caacaggatg ttatttcacg tacttttacc caggcgctct gacgccggag gatgtatgaa
     7861 aaagattatc gaaacgcaac gtgccccagg cgcaatcggc ccttatgttc agggcgttga
     7921 tttaggcagc atggtcttca cctccgggca aattccggtt tgcccgcaga ccggtgagat
     7981 cccggctgat gtgcaagatc aggcgcgttt aagcctcgaa aacgtcaaag cgatcgtggt
     8041 tgctgccggg ctgagcgtgg gcgatatcat caagatgacc gtgttcatca ccgatctgaa
     8101 tgattttgcc accatcaatg aagtctataa gcagttcttc gatgagcatc aggcgaccta
     8161 cccgacccgg agctgtgtgc aggttgcgcg tttgccgaaa gatgtgaagc tggaaattga
     8221 agctatcgca gtacgtagtg cgtaaagcct cgtgagcggg acggtcgcaa ggtcgttccg
     8281 ctccacttca ctgaacggca atccgagggt gtggatatga ttagtgcatt cgatattttc
     8341 aaaattggga ttggtccctc cagttcgcat accgtggggc caatgaatgc cggaaaaagt
     8401 tttattgatc ggctggaaag tagcggctta ttaaccgcga cgagccatat tgtggtcgat
     8461 ctgtatgggt cgttgtcact gacgggcaaa ggccatgcca cggatgtcgc cattatcatg
     8521 ggactggcag gaaacagtcc gcaggatgtt gtcattgatg agatccctgc atttatagag
     8581 ttagtaacgc gcagcgggcg gctgccagtg gcatctggtg cgcatattgt tgattttcct
     8641 gtagcaaaga acattatctt ccatcccgaa atgttgcctc gccatgagaa cggaatgcgg
     8701 atcactgcct ggaagggaca ggaagcgcta ttaagtaaaa cctattactc tgtcggcggc
     8761 gggtttattg tcgaagaaga acacttcggc ctgtcgcacg atgtcgaaac gtccgtacct
     8821 tacgatttcc actcagcagg tgaactgctg aaaatgtgtg attacaacgg cctgtctata
     8881 tctggcctga tgatgcacaa cgagctggcg ctgtgcagca aagcggaaat tgacgcagga
     8941 tttgcccgta tctggcaagt gatgcatgac ggtattgaac gcgggatgaa cactgaaggc
     9001 gtgctgcctg gtccgctcaa tgtgccgcgt cgtgccgtag cgctgcgtcg tcagctggtt
     9061 tctagcgata acatctctaa cgatccgatg aatgtcatcg actggatcaa catgtacgcg
     9121 ctggcggtta gtgaagaaaa cgcagctggc gggcgcgtgg taacggcacc gactaacggt
     9181 gcgtgcggca ttattccggc agtgctggct tattacgata agttccgtcg tccggtaaac
     9241 gagcggtcaa ttgcccgcta ttttctggca gcgggggcta ttggcgcgct gtataaaatg
     9301 aacgcctcca tctctggcgc ggaggtcggc tgtcaggggg agattggcgt ggcctgttca
     9361 atggcggcgg cagggttaac tgaactactg ggcggcagtc cggcgcaggt atgcaatgcg
     9421 gcggaaatcg cgatggagca taaccttggg ctgacctgcg atccggttgc cggacaggta
     9481 caaatcccgt gcattgaacg taatgccatt aatgccgtga aagcagtaaa cgccgcgtgg
     9541 atggcgatgc gccgcacctc ggcaccgcgt gtttcactcg ataaagtgat cgagacgatg
     9601 tatgaaaccg gcaaagatat gaacgataaa taccgcgaaa catcacgcgg aggactggcc
     9661 attaaagtgg tctgcggctg acattgctgt cagctttcat ccttgggtgc acaaatgtgc
     9721 accttttttt tgtgatctgc cgcaaataga gaatgaatat tctcttgcga ttaatttagt
     9781 agaaaattct tatccatctc cgttatttca aagtcttgat tgtgaagatt ttctctgacc
     9841 tcgcgcgtat catttcttta ttctaataac tcgcctgcaa aaacgtagac cgcatatcgc
     9901 cttgatgcgg ctcggaaaaa aataataagc cctacaaaat attgagcgag aaattatgga
     9961 aattgcatcg aataaaggcg tcattgcaga cgcttcgacc ccggcgggtc gtgctggaat
    10021 gagtgagagc gagtggcgag aagcgatcaa attcgacagt actgacaccg gctgggtgat
    10081 tatgagtatc gggatggcga ttggcgcggg gattgttttt ctcccggtgc aggtcggttt
    10141 gatgggattg tgggtatttt tgctctcatc ggtgattggt tacccggcaa tgtatctgtt
    10201 tcagcggttg tttattaata cgctggcaga atcaccagaa tgtaaagatt acccgagtgt
    10261 cattagcggt tatttaggta aaaactgggg catcctgtta ggtgcgttct atttcgtaat
    10321 gctggtgatt tggatgttcg tctattccac cgccatcacc aacgatagtg cttcctatct
    10381 gcataccttc ggcgtgacgg aagggttgct gtcagacagt cccttttatg gtctggtact
    10441 gatttgcatt ctggtggcga tctcttcacg cggcgagaaa ttgttattca aaatttcgac
    10501 cggcatggtg ctgaccaagc tgctggtggt cgcggcgctg ggcgtgtcga tggtgggaat
    10561 gtggcatctg tataacgtcg gttcgctacc gccgctgggg ctgctggtga aaaacgccat
    10621 tattacgctg ccgtttaccc tgacgtcgat tttgtttatc cagacgttaa gcccgatggt
    10681 gatctcttat cgctcgcggg aaaaatctat tgaagtggcg cggcataaag cattgcgggc
    10741 aatgaatatc gcgtttggca ttttgtttgt caccgtcttt ttctacgccg tgtcgttcac
    10801 gctggcgatg gggcatgacg aagcggtaaa agcctacgag cagaatattt ccgcgctggc
    10861 gattgccgca cagtttatta gcggtgacgg cgcagcgtgg gtgaaagtgg tcagcgtcat
    10921 tctcaatatc tttgctgtca tgactgcatt ctttggcgtc tatttaggct ttcgcgaagc
    10981 aacgcaaggg atcgtaatga acatcctgcg tcgcaagatg cctgccgaga agattaacga
    11041 aaatcttgtt cagcgcggca tcatgatttt cgccattttg ctggcctgga gcgctatcgt
    11101 actgaacgca ccggtgttga gcttcacctc tatctgtagc ccgattttcg gcatggtagg
    11161 gtgcctgatc ccggcgtggc tggtttacaa agtaccggca ttgcacaaat acaaagggat
    11221 gtctctgtac ctgattatcg tcactggttt gttgctttgt gtttctccgt tcctggcatt
    11281 ttcttgattt acctgaaatt ttaaggtttt taatatgttt gattcgactt taaatccgtt
    11341 atggcagcgt tacatcctcg ccgttcagga ggaagtaaaa ccggcgctgg gatgtactga
    11401 accgatttca ctggcgctgg cggcggcggt tgctgcggca gaactggaag gtccggttga
    11461 acgtgtagaa gcctgggttt cgccaaatct gatgaagaac ggtctgggcg tcaccgttcc
    11521 cggcacggga atggtggggc tgccgattgc ggcggcgctg ggggcgttag gtggaaatgc
    11581 caacgccggg ctggaagtgc tgaaagacgc aactgcgcag gcaattgccg atgccaaagc
    11641 actgctggcg gcggggaaag tctccgttaa gatccaggaa ccttgcaatg aaatcctctt
    11701 ctcacgcgcc aaagtctgga acggtgagaa gtgggcgtgt gtcaccatcg tcggcgggca
    11761 taccaacatt gtgcatattg agacgcacaa tggtgtggtg tttacccagc aggcgtgtgt
    11821 ggcagagggc gagcaagagt ctccgctgac ggtgctttcc agaacgacgc tggctgagat
    11881 cctgaagttc gtcaatgaag tcccgtttgc ggcgatccgc tttattctcg attccgcgaa
    11941 gctaaattgt gcgttatcgc aggaaggttt gagcggtaag tgggggctgc atattggcgc
    12001 gacgctggaa aaacagtgcg agcgcggttt gctggcgaaa gatctctctt catccattgt
    12061 gattcgtacc agcgcggcat ccgatgcgcg tatgggcggc gctacgcttc cggctatgag
    12121 taactccggc tcgggtaacc aggggatcac cgcaacaatg cctgtggtgg ttgtagcaga
    12181 acacttcgga gcggatgatg aacgactggc gcgtgcgctg atgctttctc atttgagcgc
    12241 aatttacatc cataaccagt taccgcgttt gtctgcactt tgtgccgcaa cgaccgcagc
    12301 aatgggggcc gccgccggga tggcatggct ggtggatggg cgttatgaaa ctatctcgat
    12361 ggcgatcagc agtatgatcg gcgatgtcag cggcatgatt tgcgatggtg cgtcgaacag
    12421 ctgcgcgatg aaggtttcga ccagtgcttc ggctgcgtgg aaagcggtgt taatggcgct
    12481 ggatgatacc gccgtgaccg gcaatgaagg gatcgtggcg catgatgttg agcagtcgat
    12541 tgccaacctg tgtgcgttag caagccattc gatgcagcaa acggatcggc agattatcga
    12601 gattatggcg agcaaggcca gataaacgcc aggttttgag tattgtgccg gatgcgatgc
    12661 tggcgcacct tatccggcct acgggttgtt taattcgtta atgttgttgg attttgtagg
    12721 ccggataagg cgttttcgcc gcatccggca acctgtcttt acgcggtagc ttcatcctcg
    12781 gctaaacgcg tttcagcatc cgcgacgatg gcttccagct cgctcaacat ctcttcatag
    12841 ccaaaatttt tggcggtgcg agcaggttct ttcgccacca cgggcttcac tggctggcgc
    12901 tttttggccg attttttact catcatcatc tccctgtaaa aacaactaga caggcaaatc
    12961 tatcagcaaa gcgcgcagtg gggaatcggc aaccagcgta atgttagcct cgtcacgaat
    13021 aaacgcccca tcaccgcagg tcagcgccgc tttctcttca tgatgcgtaa gcgcatgaaa
    13081 tttcccgtga atcgattgca aatacgcgcg tggcccatgc aactggaaat tcgcactttc
    13141 gcctttgtcg agcacgatat ggtgcagcca cacttgctgg cgtaattgca ggcttcccat
    13201 cgtaccctct ggcgaggcga ttaattgctg cttgcccata ttaagcgcca gtttttgaat
    13261 cagcggattc tctcgctgcg ggcaggcgtc cagccaaagc tgcattcgtg ttaacggttt
    13321 gtctttgctg agattgtgtt cgctgtagct gacacccggc tgggtagaga gcagcaacgc
    13381 ctcaccggcg ctggcctgaa catgattgcc ttcgctgtcg cgatactctg cttctccatc
    13441 cagaatcaca tttaaaatat cgactttggg ataggttcgc ggctgaaagg cggcacctgg
    13501 ggccagcact tcctggttaa gcacacgcag ggaggcatag cctaacaatt tcgggtcgaa
    13561 gtagtgtcca aaggaaaaag tataccgggc ctgcaaccat ccgtagtctg cttgtccaca
    13621 ctgcctggca gttcgggtag taatcataat tcttgacctc tctttacttc cttttatggt
    13681 aaggggctgg acgctacatt gttagccaga tattctgccc ggtatgttca aatttcctga
    13741 atgagaacga aatggccaaa gaaagggcat taacgctgga agcactacgg gttatggatg
    13801 cgatcgccgg ggcagttttg cggcggcggc ggatgagctg ggacgcgtgc cttccgcact
    13861 tagctacacc atgcaaaaac tggaagaaga gctggatgtg gtgctgtttg accgctcggg
    13921 ccatcgcacc aaattcacca atgtcgggag gatgttgctg gagcgagggc gcgttttgct
    13981 ggaagccgca gataaactga ctaccgatgc ggaagccctc gcgcgcggtt gggaaacgca
    14041 tctcaccatt gtgaccgaag cgctggtacc gacacctgcc tttttcccgt taatcgacaa
    14101 actggcggca aaagccaata cccaactggc aatcatcacc gaagtgctgg cgggggcgta
    14161 ggaacgactg gagcaggggc gggcggatat tgttatcgcg ccggatatgc attttcgttc
    14221 ctcgtcggag atcaactcgc gcaagctcta tacgttaatg aacgtctacg ttgccgcgcc
    14281 tgatcacccg attcatcagg agccggaacc gttatctgaa gtgacgcgcg tgaaatatcg
    14341 tggaattgcg gtagcggata ccgctcgtga gcgcccggtg ttgaccgtac aactgctgga
    14401 caaacagccg cgcttaacgg tgagcacgat tgaagataaa cgtcaggcat tactggcggg
    14461 gcttggcgtg gcgacgatgc cgtatccgat ggtcgaaaaa gatattgcgg aagggcggtt
    14521 gcgtgtcgtc agcccggaat cgaccagtga gatcgatatt attatggcct ggcgtcgtga
    14581 cagtatgggg gaagcgaaat cctggtgtct gcgggaaatt cccaaacttt ttagcggaaa
    14641 ataatattta ttattgttaa taaaaagggg cgaaatatcg ccccaattct aagattaatt
    14701 tgaaccaaac ttcggatcgt ttccgtaacg attgctgcca gaagtacctt ctgtgcagaa
    14761 aaaaaccaga agcacgagcc agccaataaa cggaacaaaa aagagcaagg cccaggcacc
    14821 tgaacggtcg gtatcgtgta agcgacggat agcaagcgca aggacgggaa ggaaggttgc
    14881 cagcaaataa agcatagaca gatatggaag ctccagacct aaaatcaatt gaatgacatt
    14941 gataatagcg ccgacgatgg cgttaatcag agtaaacatc cagtactctt tacgacgtgc
    15001 gcgaccagag aaaccaacat aattttttaa tacggataaa taccactgca taaaaacatc
    15061 cctttaatta aaagtgacga aagtctattc cgggtattgt gtaaatgcta ttcgtatctt
    15121 tctttttcgg atgttataaa ggttttgata tttaagataa atacatggtt attttttata
    15181 ggtaatttat ataacgacat tgaaatattt taaataaaca ttagtaaatt attcattcat
    15241 caacatgatg ataaaagccg gatgagtcag acatccggcg agcagatttg ttttaaggtt
    15301 ccagttttgg atcaggtcca aagcggtttt cgcttggcgt acccgcctgg cagttaaaga
    15361 cgatgataat aagccagccg ataaacggga ttaagaatag cagtgcccac cacgccgagc
    15421 ggtcggtgtc gtgcaggcgg cggaactgaa ctgcccacca cggtaaaaac accagaatac
    15481 cgtagattgt cgtcaggatg ccttcgccac cagcgcgttg ccagcctaac attttatcca
    15541 gcagccccag cacgaacgta aagatgatgt tgaccagaat aaacatccag tactctttgc
    15601 gccgcgcacg accccggaaa ccgacataat tttttattac tttcagatac cagtccattt
    15661 ttgcctcaat tttgctcaat cacttgtttt taaagcagca aattaagaat agacgtgaat
    15721 gaaatggcag taaatattcg ttcaaggaat taataaagtg ctgtgaaggc gcgctaagac
    15781 tgatgacaag cgcaaaatcg ccagatgcgc tacgcttatc tggcctgcgc ggtcattgca
    15841 acatattgaa tataaatgtt ttgtaggttt gataaggcgt tcacgccgca tccgatatga
    15901 acaacatgca cttagctaat tatcagaggc acttcgtaag gtgccttttt ttaaccgaag
    15961 cgaacatctc gtcctgcggt tcatcgagat cctgccacgg accaattgaa ataatccccg
    16021 tagggttgat ggtcttatgg ctgcggaagt aatgattacg gatgtgatcg aaattgacag
    16081 tttcggcgat ccccggcatc tggtagatat cgcgcaggaa gccatacaga ttcaggtaat
    16141 cgctgatgcg gtgcttatca cacttgaagt gggtcacata cactggatca aaacgcacca
    16201 gcgtagtcca caggcgaata tcggcttcgg ttagctggtt gccggtcagg taacgatgct
    16261 ggcctaaaat ctgctccagt cgcgccagcg attcaaacac tttcgccacc gcctcgtcgt
    16321 aggcttgctg gctggtggca aaaccagctt tatacacgcc gttgttaacg gtgtcataaa
    16381 tccagccgtt aagttcgtca attttcgttt gcagggctgg tgggtagtaa tcacccgctt
    16441 tcgcgcccag cgcatcaaac gcggtattga acatgcggat gatttctgtt gattcgttgc
    16501 tgacgatggt gtggttcttt ttgtcccaca gtacgggaac agtaactcgt ccgctgtagt
    16561 gtggatcggc gtggagataa agctgataca gaaattcatg ttgatagagc gtatctccgg
    16621 ttgctcccgg aaaactgtca tcaaaggtcc agccgttttc cagcatcagc gggttcacta
    16681 cggaaacgga aataaacggt tccagtcctt tgagtttgcg catgatcagc gtgcggtgcg
    16741 cccacgggca ggcgagtgaa atatagagat gataacgatc tttctctgcg ataaaaccgc
    16801 ctgtgccagt ggggccaggt gcgccatcgg cagtgagcca gttacgaaat gcggaagctg
    16861 aacgttgaaa tttaccgccg gtagatttgg tatcgtacca ggtgtcatgc cagacgccgt
    16921 caatcagttg acccattttt ctctcctcca atagcaaaaa cgaggagctt cctcctcgtt
    16981 ttttatcgtt aagtatagcg cgtgcttacc actttttatt cagcaggcgg tcgatgctat
    17041 acgcgcccgg accggtaatt gccagcagca ggaatccgcc agaaattgtc aggtttttca
    17101 tgaacatcag cgagttgacg ccttcagcaa agttgctgtg aaataaaaat gccgtcagca
    17161 gcgtaaagcc cgcagtaaac agggctgtgg tgcgagtcag gaaaccgaac aggattgcca
    17221 gaccaccacc gaactcaagc agaatcacca gcggcagcat aaaacccggg acacccattg
    17281 cttccatata ttgttgggta ccagagtaag cggtgatttt tccccagcct gcggtaataa
    17341 acagaatcgg cattaaaatg cgcgctacca gtacaccaac atcttctaat tttttcatca
    17401 tactctccac tgcgccactc aggctgctga ttgttttttt gtgcaagggc gcggtattag
    17461 cgtcgttgct gtcgatggag agaatcataa acgtggtgaa tgatgattgt tagcaaggaa
    17521 aactgtcaaa aatcttcaaa aaatttgagg gataaggccg gaattgctcc ggccagaggg
    17581 aagttaaccg cgaagctgtt gctgcttgag ggtcgtttta accagacgcc aggcgctcca
    17641 tacgccaaaa ccgcgtctgg cccagcggac cagcatatta ggatggcgaa tcgtccagat
    17701 cgccatcacg ctactgccaa ccagcgccca ggagcgcaga cttagcagca tattccagcg
    17761 acgatcgtaa gcgcctgttg cctccagcca ttcacgacga ctggcggaaa gatccagccg
    17821 ttgttgctgg atctggctaa gcagttgcgc cttacgtcgt tcacgttcga ctttactgct
    17881 cacgggactc ctcctcgagc agttgccgat cgtttgccag ctcatggcgt gtatggcgca
    17941 gcaaggtgga tttacgcgat ttacgtaacg tccagatacc gccaatcagt gccagtagca
    18001 gcaacaccac ggtggtggca atcatcgcat tcaggcgata ttgcggatca accgcccaaa
    18061 taattagcac catcaggctc ataagaccaa atgcagcgaa aagcatcgtc aggcccagca
    18121 tcagtaaaag ttgaaagaga ttagcttttt cctcttccag ctccaccacc gccagccgca
    18181 gacgtgtttc caccatttca accatgatgg aaacaattcg ctgcccgatg cccagaacgc
    18241 ttttaccggg cccttgcgcg tgatgagtgt ccgccataat taacgacgcg acagcagaac
    18301 gccgagcact acaccgattg cagcgccaat tcccacgccg gtccacggat tttcacgcac
    18361 atactcatca gcacgcgccg ccgcgacacg ggtttgtttg gcgatggcat caccggtttc
    18421 acccaggcga taacggctct gtttcagtgc ctgctccgct ttgctacgaa tcttactcaa
    18481 ctcttctttc gacttctcgc cagatgagct aagcacctct tccagcgtat cggaaaggga
    18541 tttcaactca gcacgcagat gttccgtagt gtgttctttc gacatagttt tctccaggtg
    18601 agtaagtagt gactattgtg agttagtagt cgcgcgcttc cagctttttc agctcttcct
    18661 gcgcttctgc cagtttccgt tcgcgtttgg caatcttatc ggcatcgcct ttttgcttcg
    18721 cctcggctaa atcttgctgg cgttccgcca cctcatcttt ctgctttgcg attttcttgt
    18781 gatgatcggc acgcagctgg ctatctgaac agttggcccg gacttcactc agggctttat
    18841 tcagaccgtc aatacgattc tggttttggt gtttttcggc atagctgatc tccttaagga
    18901 tattttgctc cttttcctga cacagggtag tggcataact accggcacta agagcaaaga
    18961 gagaaacagc taaagcgatg cggtatttca ttcttgaacc ttccattgtg attcggacct
    19021 attacaaagg aacgattgtc cagacaacga agcggtagtg ttaaccaccg cctcattaat
    19081 catagacagt tacggtgaaa tcaccaaagt gagtgatatg tttcggataa caggaagtta
    19141 tccgaagcga tgagagttat cccgtaaccg ggtcagccac tgcatagcct gactgttatt
    19201 gtcctgctgc gcaatgatgt agccatgggg caatgttctg tcgaggaccg cttttgcggc
    19261 ggcgctctgg tcgctggagt caaatgtaat taacagagtg tcgtttttgg gggtgatact
    19321 tttgaaagga atgccatgag cgtcaaggtg atgccagatc gaaaaaccgt ctggcatcgt
    19381 tgtgccttga tgaacggcac gaatcgccag cgtagactct tgctggcgaa ccgcggacca
    19441 ggccagcaac agcgtgccga ccagaagtaa aacagtgccg gaccaggcta gctggcgaag
    19501 cgacatgcga ggtatttgca tcccttaccc ccgatttcca tatttctttt tccataacac
    19561 gaccagagaa cctgccaggc caaacaccag cagcaccacc gggagcagca tcaggcatga
    19621 catcagctgg tcctcgtact ttaaaaatat cggcgttttg ccgagcatgt aacccagagt
    19681 tgtcaggatc aatacccaca gcagaccgct catccagttg aaaaactgaa agcgcgcgtt
    19741 attcagccct gataacccgg caatcgtcgg cagcagtgtt ctgacaaacg caataaagcg
    19801 accaattaac agcgccgata aaccgtgttt atgaaaaaga tggtgtgcgc gttgatgata
    19861 atgcgcgggt aaatgagata gccagttttg tacggtgcgg gtattgccca gccatcgccc
    19921 ctgaatatag ctgacccagc agccgaggct ggcggcaacg gtcagcagca gaatcgtttg
    19981 cgggtaaccc atcgcgcctt tcgcaatcaa cacgccgacc aataccagta aactgtcgcc
    20041 cggtaaaaag gccgctggaa gcaagccgtt ttcaaggaac aaaattacaa acaagacaaa
    20101 atacaacatg ccaatcatcg atggattggc cagggtttca aaatcctgcg cccacagggc
    20161 ttgcagcaat tgggtcaaaa gttccattca ttattcctgg tgaatcggtt aacgccacgc
    20221 tggttttaaa agatgcacta ccgcaatgtt attgctgttt tggtatgttc tgcgggtttg
    20281 atacatcaac acagcctgaa ctattccctg ttaaatcgtg agataagaaa gcattcaaaa
    20341 acataaattc aaattgaatc taattgtaac aaaggtcaac gttgttcgtt atcaaaatta
    20401 catgtccgtg gagtctgatt ttgctgaatg caggggaggg tggcgagagg atttacacag
    20461 gctgacactt tatacttttc gcttaccgac gaagcgaaca ggcgcgtcgc agcgcctgtc
    20521 ggacaggagt ttcatttact gccactggtg gccgtatcga gatgcactac cgggttttcg
    20581 gcgaatagat agcggtcggc attgaactcg aaatcgtcgc tggtttcgtt aaataacatg
    20641 attttggtgt tttccagatg ctgccacatt gccagcttag cagcatgggg atctttgcga
    20701 attaacgcct tgagaatttg atcgtggtca tcacaccagt tatcaacggt acgggaatca
    20761 atgtgttcgt gtagtttttt ccagtacggg ttatggctac gctgggtcca cattttttca
    20821 acgatggccg ccagggcgga gttctgcgtc gccagggcta cctgaatgtg gaactgtaaa
    20881 tcccactcgg aatcacggaa gcattgttcg ccgcgcgcct gttcctgaat ggccatcagt
    20941 ttcatgatgt cttgtttcgt tacctgagtt gccgcgaact cggcaatatt actttcgatg
    21001 agctggcgag cctgaagcaa ctcaaacgga ccgtaattgg cgaattccat attattgtca
    21061 gccgcctgct gatggcgcgg ctggttggaa accacatgaa tacctgaacc tttacgcacc
    21121 tccacatagc cttcaacttc cagcatgatg atggcttcac gaactaccgt tcggctgacg
    21181 ttcttttcat cggcaataaa gcgttcggca ggcagtttat cacccaccag atagacgccc
    21241 tgttcaatgc gctctttcag gtcagcggca agttgttgat acaaacggcg tggttcagtg
    21301 atttccatat gcgtctcccg gagtggaatg cggacgaagt tatcatacca ctttattcat
    21361 gggtatgcca ttggtcatat ataaatttgg tgattgaatt aatgtattga attaatattg
    21421 tgaatattta taaattccta aaaaataagt atcgtcattg tgaaagctac taaatttcag
    21481 taagttaatt cccagccttt aaatttaata ttaactaaaa atgctttttt atattgactt
    21541 acaataaatc tacatctcta atacaccaac tttttggaaa caatattgta agatcaaatt
    21601 tatatggtgg cgaaaattaa tctcttatca cggcttacgc cgcttttatt cgtgttcgct
    21661 ccttttttgg cacagtcaaa tatgactgtt tatcctatgg ctgtttccat taatagtcag
    21721 ggtgaaggca acgttcgcgt aatatctaaa agtaatgaag ttcagtacat caaggcgaca
    21781 gtattccgta tcgataatcc atcgacgcct caggaaaatg aagttgagat taaatccggt
    21841 gatgcaaatc atctggttgt tatgccacct aaattcgctt taccagccgg tagtagcaaa
    21901 actgtgcgct ttgttgcaat ggaaccagag caaaaagaaa aaaattatcg cgttaaattt
    21961 gaagcggttc ccagtattga tgacgttgcc acagataaaa aagatctctc tatgcagtta
    22021 acagttaact taatttgggg gattgttgtt agtgttccac ctcagcaacc tattgctaag
    22081 ttagaagtaa atgctgccca aaaattagtt aatgcaggga atcaacgctt aaagatttta
    22141 acgatcgctt attgtaaaaa taatagcaaa gaaaattgta agatacagac cgtaaataaa
    22201 aacatcttcc ttggtcagga aagaaatctt gaaagcatat ctggctacga caaaatcgtt
    22261 gtcaaatata ataactggat caccaaagat aatggcgagt ttgaactggc ggtccattaa
    22321 ttatcattgt aataaacgaa ggaatagtaa tgaaaaaggt atttgcaaaa tctcttctgg
    22381 tcgcagcgat gttttctgtt gcaggctccg cattggcagt acaaaaggat attactgtaa
    22441 cggccaacgt tgatgccgct ctggatatga cacagaccga taacaccgcg ttgccgaaag
    22501 cagtagaaat gcaatatctg ccggggcagg gtctgcaatc ttaccagttg atgaccaaaa
    22561 tctggtctaa cgacgttacc aaagatgtaa aaatgcagtt agtttccccg gcgcaactgg
    22621 ttcaaagcct ggatgctagc aagattgttc cgctgacagt aacctggggc ggtgaagaaa
    22681 ttaaagctga tgcggcaacc acttttaccg caaccaaaat ctttgcttcc gatgcgttaa
    22741 ctaacggttc tctggctaaa aacctgatgt ttgctcaaac caccaaaggt gttctggaga
    22801 caggcatcta ccgtggtgta gtaagcattt atctgtctca ggatatctaa tccagataac
    22861 agaattaaca cgtaacaaag ggaggggtag ccctccctta ttttaactgt tttagtattt
    22921 gttatggatt tcaggattgc catggataaa aaattactgg ctcttttgat cctggcgagt
    22981 ctcagcccgg cagaggcgac attaaccgaa attcccgcag ggtttgaggt tattgctcag
    23041 ggacagcagg agtatatcga ggtttatttt tcagggaaaa gtctcggtaa atattatgca
    23101 atggttaatc ttgataccgt aacatttctt gatccagcaa gtctatataa caagctggaa
    23161 ctggatgtag acgatcagaa aatcgcgcat atagtgaaag aaaaattatc gcagccgcta
    23221 gctcgccacg gtgaattggc ttgcggttat gtacgtactg actcagggtg tggttttctg
    23281 aataccgata cgctggaaat aatctataat gatgaagaaa gttcggcaac gttgtttatt
    23341 aatccgcaat ggaattcagc tttcgatgcg aagtcattat atttaaatcc agacaaaaat
    23401 actgttaatg cttttataca tcagcaagac atcaatgttc tggcacagga tgattaccaa
    23461 tcgttgtcta ttcagggaaa cggtgcgctg ggaataacag aaaatagtta tattggtgca
    23521 cactggaatt tcaacggtta tgatgcagat gatgtcagtg acagtaatgc tgacgtcagc
    23581 gatctctatt atcgttatga ttttttacgt cgttattatg tgcaggcggg tcgcatggac
    23641 aaccgcacac tatttaatgc acaaggcggg aactttacct ttaactttct gccactcggt
    23701 gcaattgatg ggatgcgtat cgggtcgaca ctgagctatt taaaccaggc gcaaagccag
    23761 caaggaaccc cagtaatggt tctgctttcg cgcaattctc gtgttgacgc ttatcgtaat
    23821 gagcaacttt tgggatcgtt ttatctcaat agtggttcgc aatttattga taccagttcc
    23881 tttccgccag gcagctacag cgtggcatta aaagtctacg aaaataacca actcacccgc
    23941 accgagcttg tgccgtttac caaaaccggc ggtctgactg acggaaatgc gcaatggttc
    24001 ttacaggcag gtaaaactac atcacaggtt tctgatgatg aaagttcagc ttatcagcta
    24061 ggagtacgcc tgccattaca tccgcaatat gagctctacg cagggctggc gaatgccgat
    24121 gatgtgagtg ctttcgagtt aggtaataac tggacggcag atttaggcgg ggtggggaat
    24181 cttgcaatca gcgccagcgt gttccgtaac gatgacggcg gcaaaggtga tatgcaacag
    24241 gccaactgga gtaatccggg atggccgacg ttgggctttt atcggaccaa ctctgacggc
    24301 gatgcttgta caaccgacag cagagagagc tataacgcct taagctgtta tgaaagtatt
    24361 tccgcgacgg tttcacagaa ttttgtcggc tggaatatga tgctgggtta tacccgcaca
    24421 caaaataaca ctgatgatag tttgcgttgg gataaacagc agagctttga aaataactat
    24481 cttcgccaga caacagcgca aagtatctct gaaactgtac aacttagcgc ttcccgcgct
    24541 tttgtgatgc gtgactggat tttgagtacg tcagtcggtg ttttccatcg taatgacaac
    24601 ggtggcgata acgacgacaa cggcttgtac ttatcgtttt cgttatctga cacgccaacg
    24661 atggatagca ataacaacag ccattcaaca aatgtgtcta cggattatcg ttatagcgaa
    24721 caggatggcg atcaaacgtc atggcagtta tcgcatacct tttataacga ttcattcagc
    24781 cataaagaac ttggcgtaac cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt
    24841 aacgggcgtt gggatgggca atacggaaat gtctacgcta ccgtatctga tagttatgac
    24901 cgtaagaatc atgatcatct ttcggccttt acgggcactt atagctctac gctggctgtc
    24961 agttgctatg gcgttaattt gggagccagt ggtacagacg atttgctggg tgcggtactg
    25021 gtggatgtga aaggcttctc tgaacaggat gaagagagtc aggatctgca actcgaagcg
    25081 cgggtggcag gcagccgaac tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat
    25141 cctggatttc agtctggttt tgttgaggtt aacgacagta gccagggcaa tcagcagggg
    25201 acaacaaaca tcattaacgg tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg
    25261 cgctatcgcg aagtgtctgc cagctttaac tacaactata tcggtcgttt attactgccg
    25321 gcagcggtga aaaaattccc gattgtgggg ctgaacagcg ccatgttact ggtagctgaa
    25381 gatggaggat ttacacttga aattaacggc agcgaaaaag agctctatct gctttccggg
    25441 cagcaattcc ttaagtgtcc actgagtgtt gtaaagaaac gcgccagcat tcgttacagc
    25501 ggagatgtta cttgtagtgt ggtgacttat tcacaattac cggagtcgat tcaggttcag
    25561 gcacagttga aacagcctaa attacgtgga aacgttcaga cggcgcaaag ggaggttgca
    25621 ccatgagaaa tcgattgatt gcggcgatat tgggcttgtt tggcacgctc attggcgttc
    25681 aggcagctcc tgacgtgacc agtgaaatta cgtatgattt ggcatctggt agagcggatt
    25741 attacttctg gaaggatgaa gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt
    25801 cgtatcctga cctccaacaa acctgtacag ctaatggaaa tatatcgaca gtacaaatct
    25861 atttaactga acaacgcagt gggatgcgtt ggccggtaaa actcaaagga tttaaaacag
    25921 ccattgtaag tagcgatgaa gcgccgccag gatgcaaggg gggcaaaggg cttcagacga
    25981 atcttaagga ttctaataga tcttcatgta cagaagatgg tcaacattat tatatatacg
    26041 atacaaagtt tcttacgctc taccttgagc agacagagat gaagaatttg ccgattggtg
    26101 gcgtctggaa ggggaaagtt aaattacatt cgaacagccc ggcccaggac tatttcgcaa
    26161 atattaccct gaatacgctc gaccccaacc atattgacgt gttcttcccg gagttcgccc
    26221 acgccacgcc aagggtgcag ttagacttgc atccaacagg aagcgttaac ggcagcaact
    26281 acgcgcaaga tctgaccatg ttggacatgt gcctgtacga tggttttaac ggtaatgcca
    26341 tcagttatga aatcatgctc aaagatgaag ggcgaccagc tgcagggcgc agagacggtg
    26401 acttctctat ctatcgtcaa ggagggacca ccaccgacga gggagaacgc attgattacc
    26461 gggtcaaaat gtacaacccg gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata
    26521 tggtctggaa cagcattaac ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct
    26581 atgccgtgat gtgtgtgccg acacccttga ctctggcagt cgataaattc agcgtgatgg
    26641 ataaacaggc cgggtactac atgggcaaat tgtcagtaat ctttacgcct tccttgccaa
    26701 ccatcaattg accccgcaga ccagaaaaaa ctaaacaacg aagccgcctg ataaggcggc
    26761 ttcgtcatta caacttacgt taaacgttaa acgttaatgt tgcggtgcgg gatcgttatg
    26821 tgtttcctgc gccacctcaa tcgccggttt gttctgcaat acggtccaga taaccagcgc
    26881 acccagcagg tcaaatactg ccagaactgc gaacagcggg ctgaagccga tggtgtcagc
    26941 cagtgcaccg acaaccagcg caaacagcgt acttgccagc catgcggaca tcccggttaa
    27001 accgttcgcc gtcgccactt cgttacgacc gaacacgtca gaagagagcg taatcagcgc
    27061 gccagacagt gcctggtggg caaaaccacc gatacacagc agcataattg cgacatacgg
    27121 gttggtgaac agaccgatca tacccgggcc aatcatcagc acagcaccca gcgttacgac
    27181 catcttacgg gaaacgatca ggttcacacc aaaccaacgc tggaacagcg gcggcaggta
    27241 accaccgagg atacaaccga ggtcagcaaa cagcatcggc atccaggcga acatcgcgat
    27301 ctctttcagg ttaaagccgt aaactttaaa catgaacagc gggatccacg cgttaaaagt
    27361 accccaggcc ggttctgcca ggaaacgcgg cagtgcgata ccccagaact gacggttacg
    27421 caggatctga ccaacggaca ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc
    27481 attaataata tagtcgcgtt cttcatcggt cagatgcttc tggtcgcgcg gatgtttata
    27541 gaaaatcagc catgccatcg cccagataaa gctcaatgca ccggagatga taaatgccat
    27601 ctgccagctg tgcatcacga ttgccatacc accagcggcg gcgcaatcat cgcaccaatc
    27661 gaagaaccta cgttaaagta gcctactgcg atggaacgct ctttcgccgg gaaccattcg
    27721 gagctggctt tcagacccgc cgggatcatc gctgcttccg cggcaccgac cgcaccacga
    27781 gcaacagcca ggccacccca gctacctgcc agcgcggtta caccacagaa cacggcccac
    27841 agcacagcaa acattgcata accgattttc gtacccagca catccagtac ataacctgct
    27901 accggttgca tgaccgtata agcagcagaa taggctgcga tgatatagga atactgttgg
    27961 gtggagatgt ttaactcttc catcagagtt ggcgcagctg ccgccacagt gttacgcgtc
    28021 aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca tataccaacg taacccttta
    28081 attttacgca tgtaaaacct catcgttttg ttatatccgc aggaagcgcg gccatcatcc
    28141 cgccagaggc tgaagatgtt agttacgacc gggtaaccga aaaaaaaccg gcacgccccg
    28201 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
    28261 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
    28321 ataacttgtc atacaacttt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
    28381 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttatcactt
    28441 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
    28501 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
    28561 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
    28621 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
    28681 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggctgaa
    28741 aggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggccgagc gtctgtgtac
    28801 cggtgatgca tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
    28861 cggcaacccg ttataccact ggacgcacct tgaactgcgt cgtccgtttg gtatcactgg
    28921 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
    28981 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
    29041 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
    29101 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaacatcg aacaggcgac
    29161 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
    29221 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
    29281 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
    29341 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
    29401 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
    29461 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
    29521 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
    29581 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
    29641 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
    29701 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
    29761 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
    29821 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
    29881 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
    29941 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
    30001 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
    30061 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
    30121 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
    30181 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
    30241 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ctgattttca ggatctgcct
    30301 gcgcaagcgg cagatcgtga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
    30361 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
    30421 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
    30481 agccacacct acggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
    30541 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
    30601 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
    30661 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
    30721 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
    30781 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
    30841 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
    30901 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
    30961 ctggtcacca tggtcaacga ctttaaacag tactttattg cccatgatca gccgatctac
    31021 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
    31081 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
    31141 ctgaaaacgc ctgggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
    31201 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
    31261 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
    31321 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
    31381 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacttg caacgagcgt
    31441 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
    31501 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgata
    31561 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
    31621 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
    31681 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
    31741 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
    31801 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
    31861 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
    31921 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
    31981 gtcgataggc ggcacaatga ttgagggtag ttttctgctt atgaatggct tgaatatcat
    32041 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
    32101 cgccgtagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
    32161 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
    32221 tcgcaagagt cctgcaatac gccgatgata aagccgacgg caaccacctg catggcgata
    32281 tcgttcgaaa taccgaacat attacaggcc agcgggatca gcagcagaga accccccgcc
    32341 acgccggatg cgccacaggc acacagagaa gccaccacgc tcaacagcag cgccgtgggc
    32401 agatcgaccg ggatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
    32461 gctgcgcccg ccatattgat ggtggcaccc agcggaatag aaacggaata ggtatcgcga
    32521 tccagattca gcttttcaca cagtgccata ttcaccggaa tgttcgccgc agagctgcgg
    32581 gtgaagaagg catacacgcc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
    32641 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
    32701 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
    32761 agggtagaag aaaccagacc aaaaatacca atcggtgcga agcgaatgac cagtttcacc
    32821 ataaaggtga cggcattcga catatcgttg accaggtttt tggtggtctc gttaccgtga
    32881 cgcagtgcga aaccgaggcc aattgcccac accaggatcc cgatgtagtt acctttcagc
    32941 agcgcgtcga tggggttgga aaccatgctc attaccagcc cgcgcatcac ttctacaatg
    33001 cctgacggtg gcgaaatatc acccgcgcta ctggataagt gcagggtaga agggaaggcg
    33061 aagctgaaaa cgactgcggc cagagcagca gagaaggtac ccagcagata gaggaacaaa
    33121 atagggcgga tattggtttt ctgcccgtgc tggtggttag caatagatgc catcaccagc
    33181 atcaacacca ggatgggggc aacggctttc agtgcgccga cgaacaaagt acctaacaga
    33241 ccaacagctt ccgccgcggg ttttgatatc catgccagaa gaatccccag aacaaggccg
    33301 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
    33361 tgcgtagtca tttttcgatc ctttcattgt gttatcgcac atccctggtg cgttctgtcg
    33421 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
    33481 tcgggaagcg aaaaatgctg gattttaaag agaagtcata ttttgaaact taatggttac
    33541 atttgtttga cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    33601 gcaaaactat ttggaatatg agaacggttg cgctccttta atgtgctatg ccatataaag
    33661 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    33721 ctctggcgaa caaaatcgcc gctgcgcgat tccctcagct tatcccttgc ggctaccggg
    33781 tcgggcgcaa gggaacgtcc ctgtaagatg cgccctcgcc gcgcatccat gcgcgtcgcc
    33841 ccggcctcca gggaacgctt cggcgatttt gacgccacca acacccgtgc ggtctttttg
    33901 taaagagcta caagccgatt acataattaa tagctcacct ttgtctggaa tgagttatct
    33961 ctaggaatgg cttaatatac agcgaatcat tctccccgct gcttatcatg ccgataattc
    34021 acccaggcgt tgataataaa cgtcatcacc agaatgccaa acaccacgcc cagcgagacg
    34081 gcgattggaa tatggtagaa gtcgacaatc agcatcttga taccgataaa caccagaatc
    34141 accgccaggc catatttgag catcgagaaa cgctctgcta cgcccgccag caggaaatac
    34201 atcgcacgca ggccgaggat cgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg
    34261 gtcacggcga agatagccgg aatgctatcc acggcgaaaa tcacgtcgct caattccacc
    34321 agaatcagta ccagcatcag cggtgtggcg tacagcaatc cattcttacg cacaaagaaa
    34381 tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc
    34441 ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc
    34501 aggaaggcac cgaagatata caggatccag tcgaactgcg aaattagcca gctgccagtg
    34561 aagatcatga tggtacgcag aacaatcgcc ccgagcacgc catacaccag cacgcggcgt
    34621 tgtaatgccg ccggaacaga gaaatagctg aacaacatca gccagacaaa gacgttatcg
    34681 accgccagcg atttctcaat cagataaccc gtgagaaagg ccagtgcctg tggatcggca
    34741 acggcgcgac cttcagtttg caccagatac caccagaaag cggcgttaaa cagtaacgac
    34801 agcgtcaccc agaccagcga ccaggccgca gcctgtttca tggtcatggc atgtgcccca
    34861 cgacgcccct gcaacaacag gtcgatagcc agcataatgg cgacaacaac agcgaatccg
    34921 ccccatagca acggcgtgcc gacagtattc atagaagttc cttacacata aaaaaacggc
    34981 caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc cggcaaggtc
    35041 tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc ggtgaccgga
    35101 tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc ccctttgcag
    35161 gtaacaaagt atgacagacc attcagccag tcaatggtct gttacatacc ttttacactg
    35221 ttttactttg gcaattatag ttttacgcta tctgccggaa aaatcacccc agtctggcgg
    35281 cggatctcag tcagcagttt ggcggtgatg cgactgaccg ccagccctgg atgatccacc
    35341 agatgctcat ccaccagggt agcaaacagc tctgcttcgt agagcatggt attaatatgc
    35401 tgcggctggg tgagatcctg catctggctg ccacgcggca cgaagcacac tttctggcat
    35461 tcagacagtt tttcaatcac cagcgatccc gcttcgccct gaatctcgct ttccaggaca
    35521 gaatcactga cctttgagtg ctgcaacgta acgctgaaat caccgtaatc catcaccacc
    35581 acgccgtggg catctacgcc gcttgccaac aaactggcgg ttgcctgcac gcttttcggt
    35641 tcaccgaata atgccaccgc cgacgccagg cagtaaaagc cgatatccat aattgaaccg
    35701 ttagagaatg ccggattaaa ggtgttggga ttctcaccat ccaggtaacg ttgataccgc
    35761 gaggaatact ggcaatagtt gaaaaagact ttacgcaatt tgccgacttt cggcagcgcc
    35821 tggcgcagca aatgaaagtt cggcaggctg gcggttttaa atgcctcaaa cagcaccacc
    35881 tgattttccc gcgcacaggc aatggcggca tccacttccg ccagattcga cgccagtggt
    35941 ttctcgcaaa tcacatgaat tttatggcta aggaaaagtt gtgtctggga aaagtgcagg
    36001 gcattcgggc tggcaatata caccgcgtca atggcatcgc tttccgccat cgcttccagc
    36061 gaggtaaaca gatgctcgac agaaaaatca ttggcgaagt gctgggcctg ttcaaggctg
    36121 cgggaatata cggcggttaa cttgtattta ccgctctcat gggcggcctc gacgaactgg
    36181 cgagtgatcc agttcgtacc aatcacagcg aaacgtatca taacgcgtgg atactccata
    36241 aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc agatacgcat
    36301 tattctttcg ccggacagcc atgctcacta aacggcccaa tcatgaccga tggcccgaga
    36361 tatttcggct ggcgatggag gattttggcg tcaatcaccg cgctgacacg agcgagtttc
    36421 tcccgcagtt gagtataaaa accgtgcttc atattaaggt ctggcgcgtt aaacccgata
    36481 gcgtcgatgc cgtactgttt tgccagccag atggcgcgtt ggttatgaaa ttcttgcgag
    36541 ataatggtga tatggttttc cccaaacacc tttttggcac gcaccactga atcaagcgtt
    36601 gagaatccgg catagtcgca gaagatcact ttcgccggta cgcctttagc gatcaaagcc
    36661 tgctgcatac ctgaggcttc atcgtaattt ttacgtccgt tatcgccact gactaacagc
    36721 catttcactt ttccgacgtg gtacaacgct gctgcggtat caatacgtcg ggtaaagtag
    36781 cgattacccg gcctcgcccc cagcaataaa cccacgttac gcgccggaac tgcgttgaca
    36841 tcgctccagg tcagctgctt gctggcgttg accattacgc gatcggcgat aaaaatggtc
    36901 gctcctgcca caagtaacag taagcaggta atctttagag tccggcgaga aaaacaaata
    36961 cggagaagaa ggcggggctg catggtcgtt atccttaacg aaagggggga ggtcgatttt
    37021 aggcgctaaa gaaagaaaag ataacgcctg acatcatttt cagattttgt cctgcccaag
    37081 cgacaactgc gttagccaca gccgggtgtc gaactcaagc tggtggtact gcggctccat
    37141 atggcagcac aactggtaaa acgctttgtt atgctctttc tccttcaggt gcgccagttc
    37201 gtgcaccacg atcatgcgta aaaacggttc cggcgcgttg cgaaacacag tagcgacgcg
    37261 gatctctgct tttgctttca gcttgccgcc ctgcacacga gaaacagcgg tatgtaatcc
    37321 gagcgcgttt ttcagtacgt ggatcttatt gtcatacatc actttagtga tcggcggggc
    37381 attgcgcaga aactgatttt ttagatcctg ggtatattgc cagagggctt tatcggtggc
    37441 gtagtcgtgt gttcccggat agcgttttgc cagcacatcg cccagacgct gttcgttaat
    37501 cagcgttcgc acctgggaaa gtagctgctc gggataaccc tgaagataag taagattgct
    37561 cataaccgcc cacgaaaaag agaaaaaggg tatactcacg cacccttttc aggggcaacg
    37621 tcgaaatttt atcattcagg agggccgatg agccacttag acaacggttt ccgttcactg
    37681 acactacaac gttttccggc gacggatgac gttaacccgc tacaggcgtg ggaagcggcg
    37741 gatgaatatt tgctgcaaca gttggacgac acagaaatcc gcggcccggt gttgatcctg
    37801 aatgatgcct ttggtgcgtt aagctgcgcg ctggcggaac ataagccgta cagcattggc
    37861 gactcataca tcagtgaact ggcgacgcgc gagaatttac gcctcaacgg gattgatgag
    37921 tcgagcgtga agtttctcga cagcaccgcc gactacccgc aacagccggg tgtggtgctg
    37981 atcaaagtgc cgaaaacact ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg
    38041 gtcacgtcgg atacacgtat tattgccggt gccaaagccc gtgacattca cacttccacg
    38101 ctggagctgt tcgagaaagt gctcggcccg accaccacta cgctggcatg gaaaaaagcg
    38161 cgcctgatta attgcacttt caatgaaccg ccgctggccg atgcgccgca gaccgttagc
    38221 tggaagctgg aaggtactga ctggactatc cacaaccatg cgaatgtctt ctcccgcacc
    38281 gggctggata ttggcgcacg cttctttatg caacatctgc cagagaatct cgaaggtgag
    38341 attgtcgatc tcggttgcgg taatggcgtt attggtctga cgctgcttga taaaaaccct
    38401 caggcgaaag tggtgtttgt cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac
    38461 gttgaaacca acatgccaga ggcgttagat cgcagcgagt ttatgatcaa caacgcgctc
    38521 tccggcgtgg agcctttccg ctttaatgct gtgctctgca acccgccgtt tcaccaacaa
    38581 catgcgctga ccgataacgt cgcctgggag atgttccacc atgcccgccg ctgcctgaaa
    38641 atcaacggcg agctgtatat tgttgccaac cgtcacctgg attacttcca taaactgaag
    38701 aagattttcg gcaactgcac caccatcgcc acgaataata aatttgtggt gctgaaagca
    38761 gtgaagctgg ggcgtcgtcg gtaagattgg gagttacctg ccggatgcgg cgttaacgcc
    38821 ttatccggcc tacgttcagc acgttactcc caggccggat aagacgcggc aagcgtcgca
    38881 tccggcaatg tctgcccagt tctggaatcg cgcttccata aaacaaaaaa tccctctcac
    38941 catcctctac attctcttgt ttagcgtttt tctacgttta ttcttccgtc acacagataa
    39001 attcatccgt tgccaatctg tcaacggatg ttatcatatt gccaatttgt caacggacgt
    39061 gatgaagaat gcacctgata actcaaaaag cattgaaaga tgctgcggaa aaatacccgc
    39121 aacataaaac ggagttggtg gctctgggga acacgattgc taagggatat ttcaaaaaac
    39181 ctgagtcatt aaaagcagta ttcccatctc tggataactt caaatatctg gataagcatt
    39241 atgttttcaa tgttgggggc aatgaattac gtgttgtagc aatggtcttt tttgaatcgc
    39301 aaaagtgcta catacgtgaa gttatgacgc ataaagaata cgatttcttt accgctgttc
    39361 atcgtactaa ggggaaaaaa tgattgcgat tgccgacatc ttgcaagcag gagaaaagct
    39421 aactgcggtg gcaccttttc tggcgggtat tcagaacgag gaacaataca cccaggcgct
    39481 ggaactggta gatcatctgc tgctcaacga tcctgaaaac cccttgctgg atctggtgtg
    39541 tgccaaaata accgcgtggg aagaatcagc gcccgaattt gcggaattta atgccatggc
    39601 tcaagccatg cctggcggta tagccgtgat tcgtaccctt atggatcaat atggtttaac
    39661 ccttgccgat ctgccggaaa ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa
    39721 gaggaaatta acgctggaac acgctaaaaa attggcaacg cgattcggca tttctcccgc
    39781 cttgtttatt gattaacgcg acgtgccgga tgcgttgcat ccggcgctta aaataacgat
    39841 ttaaatctcc agcgccagcc gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat
    39901 agccacatcg cagccgccaa ttaaatgcac agttttcccg ctatcaatca gcggttgcgc
    39961 cagcgcgcgg tttggctctt gccctgcgca gatcaccaca ttgtccactg ctaatacctg
    40021 cgtttcgccg ttgatcacca catgcagccc gtcatcgtca atcttctgat aacttacgcc
    40081 tgggatcatt ttcacacccc gcgagagcag ggtggtgcga tggatccagc cggtggtttt
    40141 gcctaacccc tgtcccggtt tgctggcttt gcgctggagc atcacaattt gccgtgggct
    40201 acgcgggatc tgcattccct gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat
    40261 cccccactca ttacagaacc tggcgatatt ctggctggtg gattcgcccg gctgacttaa
    40321 atacatcgcc gtatcaaaac caatcccgcc acaaccgatg atggcaacct tgttgcctac
    40381 cggcgctttg tcgcgcagta cattgagata actcaatacc ttcggatgat cgatcccgtc
    40441 gataggcgga acgcgcggca cgatcccact ggcgaggatc gtttcatcga acgcctgtaa
    40501 ctgatccgcc gtcacggtgt gattgagttt cagcgtcacg cccgtcactt cgatcatccg
    40561 gtggtaatag cgcagcgttt cgtaaaactc ctctttgccg gggatctgtt tggcgatatt
    40621 aaactgcccg ccgatctcgc tgtgggcatc aaacaatgtt acctgatgcc cacgcgccgc
    40681 cgcgttaatg gcaaacgcca gcccagcagg tcccgcaccg accaccgcca gatttttttt
    40741 ctgcacggca ggaaggattg gcattttggt ttcgtggcag gcgcgaggat tcaccaggca
    40801 cgaggtgact ttgccaacga agatctgatc gaggcaggcc tgattgcagc caatgcaggt
    40861 gttgatctca tcggctcgcc ccgattgcgc ttttgacagc agttccgcat cagcaagaaa
    40921 cggtcgtgcc atcgatacca tatcggcatc gccgcgcgag agaatatcgt cggcaacctg
    40981 cggatcgtta atccggttgg tggttaccag cggcagcgag acgtggcctt tcagtttgcg
    41041 cgtgacccag ctaaatgcgc cgcgcggcac gggcgtggca atggtcggaa tacgtgcttc
    41101 atgccagcca atgccggtgt tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag
    41161 ctctaccgtt tcggcaaaag tcccgccgtc ttcgaccagg tcgagcatcg acagtcggta
    41221 gataataatg aagtcgttgc cgacgcgttc gcgcaccgca cgcactactt ctacggcaaa
    41281 tcgcatccgg ttgcggtaat cgccgcccca ctggtcacta cgctgattgg tgcgcagcgt
    41341 cagaaattcg ttgatcaaat acccttcgga ccccattacc tctacaccgt cgtatcctgc
    41401 ctcccgtgcc agttgcgcgc agtgggcgaa atcgtcgatc agttgcagga tctcttcatg
    41461 ggtgagttcg tgaggaacga aacggttgat gggggcctgc aatgcggagg gagcgaccag
    41521 atgcggttgg tagctgtagc gcccggtatg caaaatttgc agggctattt tgccgccttc
    41581 ctgatgtacc gcttcggtaa tggtgcgatg gtgtgggatc tggctggcgt cgttgagcat
    41641 tgcgccacct tccatgccaa cgcctgttaa atctggtgca atgccgccgc tgacaatcag
    41701 cgccacgccg tgacgggcgc gttcggcata aaacgctgcc agccgctcgg caccgtccgg
    41761 gtattcctcc agcccggtgt gcattgagcc catcaacacg cggtttttta acgtggtaaa
    41821 acctaaatcc agcggggcga acagcgacgg gtagctcata aaattgtcca gtatgtaaaa
    41881 taattgttat gtggtcggat gagttctaat ttagccgtcg atggagaaaa gggaaaaggg
    41941 gagtgcgggg tttgtgatgg gattcaaaaa gaatacgccc ggtggtaacg ccggatgcaa
    42001 cgcttgccgc gttttatccg gcctggggga ttgcacatgt agggcggata aggcgttcac
    42061 gccgcatccg ccagccgttg caaattactg cttacggaaa aaatcgttat acaacatata
    42121 caaatgagcg gcactccagg agaaatttgg tgcgccttgc tgtgcgccag tcagcgggtt
    42181 gtaattctcc tgaattgggc catcggcggt taatcctttg gcgtgccgga agaacgtatc
    42241 cgccagcttc agggcatcat cacgataacc gtaacgctcc atccctttca gaccaaacca
    42301 gaactgatcc acccatacgc gcccacgcca gtagatatca gcgccaaaag ccggattggt
    42361 taacgccgcc gttcccagcg ggacaaaggt gttgaactct ttaggatcga gcatcacctt
    42421 caccaccgcg tcggcgttgg cctgcgttgc cgcaccgtta aacagcggcg accagccttc
    42481 cggcccttta ccgcgctcaa caatcggttt gcccgcgcag ccgttcgcca gtggtttatc
    42541 ttcaatacgt acgtcatagt agaactgggt agtcgggtcg aacatacagg tgttgatgta
    42601 gtccgcgagc tgctgtgcca actggcgata gcgtttggcc tcttctggct taccgaggat
    42661 ggttgccatc tccgccagat aatggttatc gctgtacata tagctggcct gatccaccga
    42721 ctcctgcaat agcgagtagc ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt
    42781 caccgtccag tcgctacgtt tgccgccatt agcgacatat ttatccagct gttctttgtc
    42841 gataaaccca aagacggcgg catcatcacg ccccgattcc cacgatgcag caacctgtgc
    42901 cggaatttcc agactgtcgt acaggccttt ctccaccacg cgggcgtagt tgttcagccc
    42961 agactgcgtc tcttctttat cgcctttttt caccgtaaac agcatctcgc cgctctcagt
    43021 gttgtgggct ttgtcgcggg tcgcgccata ttccggcacg ccgttgccgt tgtgatcgcg
    43081 gttacgtaac caccagtcgt gataggccac cagtttcggg tacatctctg ccaaccaggc
    43141 tttatcctgg gtgacgttgt acacttccat caccgaccag gcggcaaggc tgggtttggt
    43201 attacgttcg ttccagttgc cgccatcgcc accacgctcg gggctaaggt tccacgctat
    43261 caggtcgggg acaaagccca catcctgcgg acgcacgcta tcgccaggct ggatctgcca
    43321 ggagaagacc gcgcggatat tctctttggc gatgtccgga ttgaaatgcg ccatcgcaaa
    43381 cgcctgcttc caggtatccc acggccaggt ctgattgccg gagaaccagc gcccggtcac
    43441 cgacggtgta acggtgttaa atttcaccgc accgcccggt gagagccagt taccgttgag
    43501 cgtttcgatc gctttcaccg cgacgcgcgt ctgttccggc gtcgcatccg gattggttaa
    43561 ccctttcttc aggtattctt cccagcgttg ctgcgaggcg gtgagataaa acgccggacg
    43621 cgccagaata tcgcggatct gcatttgctc tttgctaact tcctgagcgg tcagcagatg
    43681 ggaataggtg gtgtagagcg tggtcgaacc gttgatatgc gccttactgg taaagcgatt
    43741 accgttgatt tcagtctgca ccggcaggga tttatgcacc tgatattccg attcgccgga
    43801 ggtcagcaga tcccaggtgg cgcgcacttt gccaaaggtg actttcaggc catcacgggt
    43861 ggcgctgatt ttgcgctgat agtcagggta ttcgccagca atggttttat cggaaagcgg
    43921 tttcccttct ttcgcttcca gtttttccag cagttcgccg tcccacacca gatccagcgg
    43981 tttattgctg gtgattttgg tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt
    44041 catttcgact tgcacatctt ttgctgtcag tttttgcacc agcgcaccgg gaatactgta
    44101 tgcctccagc gtaaagtcga ctttcttgcc gtcctgccag acggtcaggc ggtcgaaatt
    44161 gctggccata aagttgatgt actcttccgt cagcagcgca acgcccggaa agccgcccat
    44221 ggtgttaggg ccgtctggca acagatgacc atgccaggct ccgagatcga aaaacggatt
    44281 aaaacgctgg tgatcgtcgt aatcgtaatc cttcatgtac tgcggcgcgc cagtacggtt
    44341 aatcacgttt ttataattgt cggcgttagc ggcatgggcg gaaaaactta tcagcagagc
    44401 gcaggttact ggcgttaaaa tagtttttat tttcatgaaa ttacagtcct tattaccagt
    44461 agaaatattg catcaggaag atttggtcgc tgggatcgtg agaggcattt ttcatgtaga
    44521 agcggctatc aaaagccgtg gcgaaacgtc cgccgccgta ttcgtaccag ataccaaact
    44581 gcatgaaaga ggtggtttca tcgtcagcgt ggtcagggcg atgtttggcg taggtgtaag
    44641 cggtggagag ataaacgccc tttaacgctt catacatggc gctcaccatc atcccacttt
    44701 cacttcccgg tacgtccagc ccgtcggcgc gtccggtatg gtgccaggcg cgagcggcaa
    44761 agttaggagt tagcaggccc gtgagataga gctgtcctgt gccgtcggtg atttccagac
    44821 cgttcatcca ggtgatgcct tcggcaaggt catactggat gtaaccgttc atcattgccg
    44881 gataggtgta tttgtcgtca tagcgatcgt atttgccaaa atgtagccag gctttgcttt
    44941 catcatgatg tccggcagga gtcgcggtga cgctataacg aaagttgccg cttaagttct
    45001 gtaccttcag cgcagtcata atgtcgcggg tattgggaat gacgtagccc agatcggggg
    45061 tgaaatcgcc ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga
    45121 tttcggtgcc atcattggta cgataaccgc catagaaacg gttaattccg ccctcgaaac
    45181 caccccagct atggtctggc acccaggcat tgccgtcgtg gtcggcctgc acggtccagc
    45241 cttcgccgta aatcaggccg aactaggagc cgtacttcat ctccaggcca ccttcaatat
    45301 aggtaccgtc gctgtattta tgtttattct caccctctaa atccatatat ccaccacccc
    45361 ctaatcccta attcgccgta aaaacgaaat gaaggagaag atgtcgaggt aattgcgggt
    45421 gtttttgtgt cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga aaaggcgtag
    45481 caaaacggca gagcaagtaa tgctctgcat ggtgcagtga ttaatttcat tgtgttaatc
    45541 catgagctaa agaaaaagta cagcctttta tcgttgtaat tataaaaata taaaggcaga
    45601 aactattttc cctctcctca tccaggaaga gggaaaatgc ctgctcattt tttattattc
    45661 tgcttaatca acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag ccgttaatcc
    45721 ctttatatat ttactgtatt tccaccacgc aaagccaagg aagataacaa taccgccgac
    45781 gttataaaaa atgatggtca gaatattcgc gccagtcggg aatgtcgaag cgacaaaccc
    45841 tacggcaaat atcgcaatca gcattgaaac aacgataata ccggtgcggc gggagcccat
    45901 acggaaatcg cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag ccagcatgat
    45961 gaataacggc ggaagcatgg acgctg
//
LOCUS       DAQSWQ010000023        44082 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.023, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000023 DAQSWQ010000000
VERSION     DAQSWQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.023"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..296
                     /locus_tag="R8G18_001326"
     CDS             <1..296
                     /locus_tag="R8G18_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEE9795078.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            791..1690
                     /gene="lysO"
                     /locus_tag="R8G18_001327"
     CDS             791..1690
                     /gene="lysO"
                     /locus_tag="R8G18_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HEE9795079.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1834..3486
                     /gene="hcp"
                     /locus_tag="R8G18_001328"
                     /gene_synonym="priS"
     CDS             1834..3486
                     /gene="hcp"
                     /locus_tag="R8G18_001328"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HEE9795080.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3498..4466
                     /gene="hcr"
                     /locus_tag="R8G18_001329"
     CDS             3498..4466
                     /gene="hcr"
                     /locus_tag="R8G18_001329"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HEE9795081.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4599..6317
                     /gene="poxB"
                     /locus_tag="R8G18_001330"
     CDS             4599..6317
                     /gene="poxB"
                     /locus_tag="R8G18_001330"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HEE9795082.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6354..7355
                     /gene="ltaE"
                     /locus_tag="R8G18_001331"
     CDS             6354..7355
                     /gene="ltaE"
                     /locus_tag="R8G18_001331"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEE9795083.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7366..8796
                     /locus_tag="R8G18_001332"
     CDS             7366..8796
                     /locus_tag="R8G18_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEE9795084.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8895..9908
                     /locus_tag="R8G18_001333"
     CDS             8895..9908
                     /locus_tag="R8G18_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEE9795085.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="R8G18_001334"
     CDS             complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="R8G18_001334"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HEE9795086.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10732..11055)
                     /locus_tag="R8G18_001335"
     CDS             complement(10732..11055)
                     /locus_tag="R8G18_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEE9795087.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11266..13035)
                     /locus_tag="R8G18_001336"
     CDS             complement(11266..13035)
                     /locus_tag="R8G18_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEE9795088.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            13684..14199
                     /locus_tag="R8G18_001337"
     CDS             13684..14199
                     /locus_tag="R8G18_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEE9795089.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            14417..15145
                     /gene="artP"
                     /locus_tag="R8G18_001338"
     CDS             14417..15145
                     /gene="artP"
                     /locus_tag="R8G18_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HEE9795090.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            15163..15894
                     /gene="artJ"
                     /locus_tag="R8G18_001339"
     CDS             15163..15894
                     /gene="artJ"
                     /locus_tag="R8G18_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEE9795091.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15901..16617
                     /gene="artQ"
                     /locus_tag="R8G18_001340"
     CDS             15901..16617
                     /gene="artQ"
                     /locus_tag="R8G18_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HEE9795092.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16617..17285
                     /gene="artM"
                     /locus_tag="R8G18_001341"
     CDS             16617..17285
                     /gene="artM"
                     /locus_tag="R8G18_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HEE9795093.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17577..18308
                     /gene="artJ"
                     /locus_tag="R8G18_001342"
     CDS             17577..18308
                     /gene="artJ"
                     /locus_tag="R8G18_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HEE9795094.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="R8G18_001343"
     CDS             complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="R8G18_001343"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HEE9795095.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(19651..20139)
                     /locus_tag="R8G18_001344"
     CDS             complement(19651..20139)
                     /locus_tag="R8G18_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HEE9795096.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(20199..21044)
                     /gene="potI"
                     /locus_tag="R8G18_001345"
     CDS             complement(20199..21044)
                     /gene="potI"
                     /locus_tag="R8G18_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HEE9795097.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(21041..21994)
                     /gene="potH"
                     /locus_tag="R8G18_001346"
     CDS             complement(21041..21994)
                     /gene="potH"
                     /locus_tag="R8G18_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HEE9795098.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(22004..23137)
                     /gene="potG"
                     /locus_tag="R8G18_001347"
     CDS             complement(22004..23137)
                     /gene="potG"
                     /locus_tag="R8G18_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HEE9795099.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(23232..24344)
                     /gene="potF"
                     /locus_tag="R8G18_001348"
     CDS             complement(23232..24344)
                     /gene="potF"
                     /locus_tag="R8G18_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HEE9795100.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(24695..25171)
                     /locus_tag="R8G18_001349"
     CDS             complement(24695..25171)
                     /locus_tag="R8G18_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEE9795101.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="R8G18_001350"
     CDS             complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="R8G18_001350"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEE9795102.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(26222..26944)
                     /gene="nfsA"
                     /locus_tag="R8G18_001351"
     CDS             complement(26222..26944)
                     /gene="nfsA"
                     /locus_tag="R8G18_001351"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HEE9795103.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(26928..27215)
                     /locus_tag="R8G18_001352"
     CDS             complement(26928..27215)
                     /locus_tag="R8G18_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HEE9795104.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            27375..27632
                     /gene="grxA"
                     /locus_tag="R8G18_001353"
     CDS             27375..27632
                     /gene="grxA"
                     /locus_tag="R8G18_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HEE9795105.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(27662..28039)
                     /locus_tag="R8G18_001354"
     CDS             complement(27662..28039)
                     /locus_tag="R8G18_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HEE9795106.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            28309..29994
                     /locus_tag="R8G18_001355"
     CDS             28309..29994
                     /locus_tag="R8G18_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HEE9795107.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(30169..30705)
                     /gene="rcdA"
                     /locus_tag="R8G18_001356"
     CDS             complement(30169..30705)
                     /gene="rcdA"
                     /locus_tag="R8G18_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HEE9795108.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            30789..31997
                     /locus_tag="R8G18_001357"
     CDS             30789..31997
                     /locus_tag="R8G18_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9795109.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            31997..32812
                     /gene="ybjI"
                     /locus_tag="R8G18_001358"
     CDS             31997..32812
                     /gene="ybjI"
                     /locus_tag="R8G18_001358"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HEE9795110.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            32898..33182
                     /locus_tag="R8G18_001359"
     CDS             32898..33182
                     /locus_tag="R8G18_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795111.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(33223..34450)
                     /gene="mdfA"
                     /locus_tag="R8G18_001360"
                     /pseudo
     CDS             complement(33223..34450)
                     /gene="mdfA"
                     /locus_tag="R8G18_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            34735..35331
                     /gene="ybjG"
                     /locus_tag="R8G18_001361"
     CDS             34735..35331
                     /gene="ybjG"
                     /locus_tag="R8G18_001361"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEE9795112.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            35389..36147
                     /gene="deoR"
                     /locus_tag="R8G18_001362"
     CDS             35389..36147
                     /gene="deoR"
                     /locus_tag="R8G18_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HEE9795113.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(36194..37396)
                     /gene="dacC"
                     /locus_tag="R8G18_001363"
     CDS             complement(36194..37396)
                     /gene="dacC"
                     /locus_tag="R8G18_001363"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HEE9795114.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            37643..38268
                     /gene="gstB"
                     /locus_tag="R8G18_001364"
                     /pseudo
     CDS             37643..38268
                     /gene="gstB"
                     /locus_tag="R8G18_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(38265..39380)
                     /gene="yliI"
                     /locus_tag="R8G18_001365"
     CDS             complement(38265..39380)
                     /gene="yliI"
                     /locus_tag="R8G18_001365"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HEE9795115.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(39491..39874)
                     /gene="bssR"
                     /locus_tag="R8G18_001366"
     CDS             complement(39491..39874)
                     /gene="bssR"
                     /locus_tag="R8G18_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HEE9795116.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            40087..41412
                     /gene="rimO"
                     /locus_tag="R8G18_001367"
     CDS             40087..41412
                     /gene="rimO"
                     /locus_tag="R8G18_001367"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEE9795117.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(41459..42780)
                     /locus_tag="R8G18_001368"
                     /pseudo
     CDS             complement(41459..42780)
                     /locus_tag="R8G18_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(42788..>44082)
                     /locus_tag="R8G18_001369"
     CDS             complement(42788..>44082)
                     /locus_tag="R8G18_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEE9795118.1"
                     /translation="IAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAV
                     GIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQ
                     TFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSE
                     FIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSA
                     ERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQ
                     LNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDD
                     FGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVES
                     EDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT        10948 a        11336 c        10770 g        11028 t
ORIGIN      
        1 cgctggtagt tgaactggta ttgagtgcag gtttcctgtt ggtgatccac ggcgcaaccg
       61 acaaattcgc gccggcaggt tttgcgccga tcgctattgg tctggcctta accctgattc
      121 acttaattag tattccggtg actaacactt ctgttaaccc ggcgcgcagc accgcggttg
      181 ctatcttcca gggcggctgg gcattagaac aactgtggtt cttctgggtg gtgccaattg
      241 tcggcggcat tatcggtggt ctgatttacc ggaccctgct ggaaaagcgt gattaattaa
      301 cgcagaaggc ctggcgtaat gtcaggcctt tttttaagat gttgttatat gttgattcat
      361 catcttgttg cgctcctttc atgagctaag ccacatattg ccactggcgc aaggagcgcg
      421 cagggggcgg ccaatcgccg ccgccccctg cccccccggg ctctggcgaa caaaatcgcc
      481 gctgcgcggt gccctcgact tatcccttac ggctaccggg tcgggcgcga ggtaacatcc
      541 ctgtaaaacg cgccctcagc ccacatccat gtgggctgcc ccggccttca gggaacgcct
      601 cggcgatttt gacgccacca acatcggtgc agtttgtaat taaagcgcaa gaatatcttt
      661 aaatgaaaac gaatactcta ctgctcctta tgcataacat ttccttttaa ttgcaacttt
      721 actcgtcctt ccgctttcag tagtgtgtca tcgcgcattt cgacttcatc tttgcaagga
      781 ctgggttttc atgttttctg ggctgttaat cattctggtt cccctgattg taggttacct
      841 cattccgctt cgccaacaag ctgcgttaaa agttattaat cagctattaa gctggatggt
      901 ttaccttatt ctctttttta tgggtatcag tctggcgttt ctcgataacc tcgccagtaa
      961 cctgttggcg attctgcatt attctgccgt cagtattacc gttattttac tgtgtaatat
     1021 tgccgccctg atgtggctgg agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa
     1081 actcccgtcg cgtattgcga tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat
     1141 tggttttgcc attggtctga gtggactggc tttcttacaa cacgcgaccg aagccagtga
     1201 atacacgtta attttgctac ttttcctcgt tggtattcag ttgcgcaata atggcatgac
     1261 cttaaagcag attgtcctta atcgccgggg aatgattgtc gccgtggtgg tggttgtcag
     1321 ttcattaatt ggtggtttaa ttaacgcctt tattcttgat ctccccatca ataccgcgct
     1381 ggcaatggcc tccggtttcg gctggtattc tctttccggt attttattga ccgaatcttt
     1441 tggtccggta atcgggagcg cggcgttttt taatgatctg gcccgtgaac tgattgctat
     1501 tatgttgatc cctgggctga ttcgccgcag ccgctctact gcactgggct tatgcggtgc
     1561 cacatcaatg gatttcaccc tgcccgttct tcaacgtact ggcgggctgg atatggtccc
     1621 ggcggcaatt gttcacggtt ttattcttag cctgttagtg ccgatcctca tcgccttttt
     1681 ctctgcgtaa tacctctctg gcggtagatc cctgccgcca aaattgcgct aaatcaatct
     1741 cccttaaagt tgcatgaaaa atccctttta tccccgcgtt aagcgtctta accttaaaca
     1801 tgtatattaa atataacttt aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact
     1861 atccgtactc cggcaggaaa cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg
     1921 gaaacttctg accttcagga tttactcatc gcggcgctgc aagggctttc tgcctgggcg
     1981 gtaaaagcgt gtgaatacgg catcatcaac cacgatgttg acagcttcgc gccacgtgcc
     2041 tttttctcaa ccctgaccaa cgttaacttc gattctccgc gtattgtcgg ctacgctcgt
     2101 gaagcgattg ccttgcgcga ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg
     2161 cgcgtcgata acccgatggc tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa
     2221 cgtcaggcag cagaatttac ccctaacaaa gataaagcgg cgattggcga aaacattctc
     2281 ggcctgcgtc tgctgtgcct gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg
     2341 catgttctcg gtcaatacga caacgatatt tatgcccagt accataaaat catggcgtgg
     2401 ctggggacct ggcctgccga tatgaacgcg cttcttgagt gttcaatgga aatcggccag
     2461 atgaacttca aagtgatgag cattctggat gcaggcgaaa ccggtaaata cggtcacccg
     2521 acgccaaccc aggtcaacgt caaagcgacg gcgggtaaat gcattctgat ttccggtcac
     2581 gatctcaaag atctctacaa cctgctggaa cagaccgaag gcacgggcgt taatgtctac
     2641 acccacggcg aaatgctgcc tgcgcatggc tacccggagc tgcgtaaatt caagcatctg
     2701 gtcggtaact acggcagcgg ctggcagaat cagcaagtgg agttcgctcg tttccctggc
     2761 cccatcgtga tgacctcgaa ctgcatcatc gacccaaccg taggcgctta tgacgatcgt
     2821 atctggaccc gcagcattgt tggctggcct ggcgtgcgtc atctggacgg tgatgatttc
     2881 tctgcggtta tcacccaggc gcaacagatg gcaggcttcc cgtacagcga aattccgcac
     2941 cttatcaccg tgggttttgg tcgccagacg ctgcttggcg ctgctgatac gctgattgat
     3001 ctggtgagcc gtgaaaaact gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc
     3061 ggcgagcgcc actacttcac cgatttcgcc accagcgtgc cggatgactg cctgatcctg
     3121 accctcgcct gtggtaaata tcgctttaac aaacttgagt ttggcgatat cgaaggtctg
     3181 ccgcgtctgg tagatgcagg tcaatgtaac gatgcttact cagcgattat tctggctgtc
     3241 actctggcag agaaactggg ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc
     3301 tggtttgaac agaaagcgat cgtcattctg ctgacgctgc tttctctggg cgtgaaaaat
     3361 atcgtcaccg gcccgactgc acctggtttc ctgacaccag acctgctggc ggtgctgaac
     3421 gagaaattcg gcctgcgttc tatcaccact gttgaagaag acatgaagca actgttgagc
     3481 gcgtaaggag tttgtcgatg acgatgccaa cgaatcaatg cccgtggcgg atgcaggttc
     3541 atcacattac gcaagaaacg ccggatgtgt ggacgatttc cctgatttgc cacgattact
     3601 acccatatcg cgccgggcaa tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc
     3661 gtgcttacac catttcctcc acgccaggcg tgagtgaata tatcaccctg accgtgcggc
     3721 ggattgatga cggtgtcggc tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc
     3781 tctggctttc ggacgcgatg ggggaattta cctgcgacga taaagcagaa gataaattcc
     3841 tgttgctggc ggcaggctgc ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga
     3901 agaaccgtcc acaggccgat gtgcgggtga tctacaacgt gcgtacgccg caggatgtta
     3961 ttttcgccga tgagtggcgt aactatccgg taacgctggt ggcagaaaat aacgttaccg
     4021 aaggctttat cgctggtcgt ctcactcgcg aactgctggc aggtgtacct gacttagctt
     4081 cacgtaccgt gatgacctgc ggcccggctc cgtatatgga ttgggtagag caggaagtga
     4141 aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt caccccagta gcagaagcgg
     4201 cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac gccccggttg
     4261 gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg
     4321 cgggtgtttg cggctgctgt aagacaaaag tggtttccgg tgaatatacg gtgagcagta
     4381 caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc tcctgccatc
     4441 cgcaggggga tttggttctc gcataatcgc cttatgcccg atgatattcc tttcatcggg
     4501 ctatttaacc gttagtgcct cctttctctc ccatcccttc cccctccgtc agatgaacta
     4561 aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg gttgcagctt
     4621 atatcgccaa aacactcgaa tcggcagggg tgaaacgcat ctggggagtc acaggcgact
     4681 ctctgaacgg tcttagtgac agtcttaatc gcatgggcac catcgagtgg atgtccaccc
     4741 gccacgaaga agtggcggcc tttgccgctg gcgctgaagc acaacttagc ggagaactgg
     4801 cggtctgcgc cggatcgtgc ggccccggca acctgcactt aatcaacggc ctgttcgatt
     4861 gccaccgcaa tcacgttccg gtactggcga ttgccgctca tattccctcc agcgaaattg
     4921 gcagcggcta tttccaggaa acccacccac aagagctatt ccgcgaatgt agtcactatt
     4981 gcgagctggt ttccagcccg gagcagatcc cacaagtact ggcgattgcc atgcgcaaag
     5041 cggtgcttaa ccgtggcgtt tcggttgtcg tgttaccagg cgacgtggcg ttaaaacctg
     5101 cgccagaagg ggcaaccacg cactggtatc atgcgccaca accagtcgtg acgccggaag
     5161 aagaagagtt acgcaaactg gcgcaactgc tgcgttattc cagcaatatc gccctgatgt
     5221 gtggcagcgg ctgcgcgggg gcgcataaag agttagttga gtttgccggg aaaattaaag
     5281 cgcctattgt tcatgccctg cgcggtaaag aacatgtcga atacgataat ccgtatgatg
     5341 ttggaatgac cgggttaatc ggcttctcgt caggtttcca taccatgatg aacgccgaca
     5401 cgttagtgct actcggcacg caatttccct accgcgcctt ctacccgacc gatgccaaaa
     5461 tcattcagat tgatatcaac ccagccagca tcggcgctca cagcaaggtg gatatggcac
     5521 tggtcggcga tatcaagtcg actctgcgtg cattgcttcc attggtggaa gaaaaagccg
     5581 atcgcaagtt tctggataaa gcgctggaag attaccgcga cgcccgcaaa gggctggacg
     5641 atttagctaa accgagcgag aaagccattc acccgcaata tctggcgcag caaattagtc
     5701 attttgccgc cgatgacgct attttcacct gtgacgttgg tacgccaacg gtgtgggcgg
     5761 cacgttatct aaaaatgaac ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga
     5821 tggctaacgc catgccgcag gcgctgggtg cgcaggcgac agagccagaa cgtcaggtgg
     5881 tcgccatgtg cggcgatggc ggttttagca tgttgatggg cgatttcctc tcagtagtgc
     5941 agatgaaact gccagtgaaa attgtcgtct ttaacaacag cgtgctgggc tttgtggcga
     6001 tggagatgaa agctggtggc tatttgactg acggcaccga actacacgac acaaactttg
     6061 cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt agaaaaagcg tctgaagttg
     6121 atgaagccct gcaacgcgcc ttctccatcg acggtccggt gctggtggat gtggtggtcg
     6181 ccaaagaaga gttagccatt ccaccgcaga tcaaactcga acaggccaaa ggatttagct
     6241 tgtatatgtt gcgcgcaatc atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa
     6301 cgaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat gccatgattg
     6361 atttacgcag tgataccgtt acccgaccga gccgcgccat gctcgaagca atgatggccg
     6421 ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctctg caggactacg
     6481 ccgcagagct ttccggtaaa gaagccgcca tttttctgcc gaccggcacc caggctaacc
     6541 tggtcgctct gctcagtcac tgcgaacgcg gcgaagagta tattgtcggt caggccgcgc
     6601 ataattatct gtttgaagcc ggtggcgcag cggtgctggg cagtattcaa ccgcaaccca
     6661 tcgacgcggc tgccgacggc acgctaccgc tggataaagt ggcgatgaaa atcaaacccg
     6721 acgatatcca tttcgcccgc accaaattac tcagtctaga aaacacccac aacggcaaag
     6781 tgctgccgcg tgaatacctg aaagaagcat gggaatttac ccgcaagcgc aatctggcgc
     6841 tgcatgttga cggtgcgcgc atctttaatg ccgtggtggc ttacggctgc gaactgaaag
     6901 agatcacgca atattgtgat tcgttcacca tttgcctgtc gaaaggtctt gggacgccag
     6961 tcggttcatt actcgtcggt aatcgtgatt acattaaacg tgccatccgc tggcggaaaa
     7021 tggccggtgg cgggatgcgt cagtccggga ttctggctgc cgccgggatg tatgccctga
     7081 aaaataacgt cgcacggttg caggaagatc acgacaacgc cgcctggatg gcggagcaac
     7141 tgcgtgaagc aggcgcggat gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg
     7201 gggaagaaaa tgctgccgcg ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg
     7261 cctcgccgat tgtccgcctg gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag
     7321 tcgccgccca ctggcgcgca ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt
     7381 ttagttctcg gtgccagtgg ctacattggt cagcatctgg tgcgcacact cagccagcaa
     7441 gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca
     7501 aacgtcagtt gccataaagt cgatctcagc tggccggata accttccggc cctgttgcag
     7561 gatatcgata ccgtctattt tctggtgcac agcatgggcg aaggcggcga ttttatcgct
     7621 caggagcgcc aggtggctct caacgtccgc gatgcgctac gtgaagtacc agttaagcaa
     7681 ttaatctttc tcagttcgtt gcaggccccg ccacatgagc agtcggatca tctgcgtgct
     7741 cgtcaggcta cggcggacat tcttcgtgaa gcgaatgtac ctgtgaccga acttcgggcc
     7801 ggaattatcg ttggcgcagg ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac
     7861 ctgccagtgt taacgccgcc acgctgggta cgttcacgca ccacgcccat cgcgctggaa
     7921 aacttgctgc actatctggt ggcgttgtta gatcatccgg ccagcgaaca ccgcatcttc
     7981 gaagccgccg gaccagaggt gctcagttat cagcaacagt ttgaacattt tatggcggtg
     8041 agcggtaagc gccgctggtt gatccccatc cccctcccca cccgctggat ttcggtgtgg
     8101 tttctcaatg tgattacttc cgtaccgccc accaccgcca gggcgttgat tcaggggctg
     8161 aaacacgatc tgctggcgga tgataccgcg ctacgtgcac tcatcccaca acggctgatc
     8221 gctttcgatg acgcggtacg tagcacgttg aaagaggagg aaaaactggt caactccagc
     8281 gactggggct acgacgctca ggcctttgcc cgctggcgac cggagtacgg ttattttgcc
     8341 aaacaggcgg ggtttaccgt taaaacgtcc gccagccttg ctgctttatg gcaggtagtg
     8401 aaccaaatcg gcggtaaaga gcgttatttc tttggcaata ttttgtggca gacacgggcg
     8461 ttgatggacc gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg cgaatattta
     8521 cagactggcg atgcggtgga tagctggaaa gtgattgtcg ttgaaccgga aaaacaactt
     8581 acgttgttat ttggcatgaa agcgtcgggg ctgggacgac tgtgttttag cctggaagat
     8641 aaaggcgact atcgtactat cgatgtccgc gctttctggc atccgcacgg tatgccgggg
     8701 ctgttttact ggttattgat gatccccgcg catctgttta tttttcgcgg aatggcaaaa
     8761 caaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc gttattcttt
     8821 catgtttccc attgcatcat gggggaaatc acggaagaat gcgcacgtca ttttctcaac
     8881 aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta ggtcgaaacg
     8941 cggtagagtt tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc aacgaggcaa
     9001 tgggcaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg accgagctgg
     9061 tttcatcaca agctaaagtg atgctcgcgg gcattgatac gctgtggcac tgctccagct
     9121 ttacctcacc ctgggggaca caacaggctt tcgatctggc taacgttcgc gccactcgcc
     9181 gcctgggtga atgggctgtc gcctggggtg tgcgtaactt tattcatatc tcttccccct
     9241 ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc cctcaccgct
     9301 tcgccaacga gtttgcccgc agcaaagcag ccagcgaaga agtgatcaat atgctttcgc
     9361 aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc ggaccgcacg
     9421 ataaagtctt tattccccgt ctggcgcata tgatgcacca ctacggcagt attctgttac
     9481 cgcatggcgg cagtgcgctg gtcgatatga cctactatga aaataccgtg cacgcaatgt
     9541 ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac aatatcacca
     9601 acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgacgag ttgaatattg
     9661 actgtcgtat tcgttccgtc ccctacccga tgctggatat gatcgcccgc agcatggagc
     9721 gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc tccaagttga
     9781 attttgactt tacgctggat attacgcgtg cgcaggaaga gttaggttat cagccgatca
     9841 tcaccctaga tgaaggtatc gagaaaactg ccgcctggct acgcgaccac ggaaaactgc
     9901 cgcgctaatc ctgcccgtat ttctccagca atgcttcggc aatcgcctga gtttctgcat
     9961 ccgcttcgcc gttatataac gtcgggcgga aatgcatctg gaatgccata atcacgcgcc
    10021 gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc aaaagctcca
    10081 gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttaaccc
    10141 gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag ggaaataatg
    10201 gccccggatc gtctttgcgc tgcggtgcga tatccgcatg tgccactacg ttttccggct
    10261 tgatgtgata acgggcaata atatctttcg ccagtggaat aagcgcctga atctgtgtcg
    10321 gttcaaacgg ggcaaaatat ttcactccgg ccgatttttg ccagccacgg ttttccagct
    10381 caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat
    10441 gccaggccag ttcttgttcc ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg
    10501 gtggtaccgc agggaccaga taatgcgagc tgacctgctt atcggtcagt gtcgccagcg
    10561 agctatcaaa atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg
    10621 cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg cctttttcgc
    10681 ctgcacaccc tgccaataac agagcggcag cgaccagcca gaagactctt ctcatcgacg
    10741 cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt gcccgaccgt
    10801 ttcgtagtcg atatcaatac cgacgacggc atcggccccc agcgcccgcg cctgggagcc
    10861 taattcctca aaggcgatct cccgtgcttt acgcagttct ttttcatacg caccggaacg
    10921 tccgccaacg atatcgcgga taccggcaaa gaaatcacgg aaaatattgg cacctaaaat
    10981 cgcttcgccg gtcaccacac cgcaatattc aacgatggtc tggccttcca gagttggggt
    11041 tgtagaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct aaagcattat
    11101 cgaactgata tgccatgttg ggaagaaggt tttgatcagt ttaggaaatc atctcattgt
    11161 gtcaaaaaca cccacttaag tctttacgat tatccgttgc ataacccgat attggtttca
    11221 ttatagggta ccgcttacct gtcttattat gttgcaccaa caacattaat gagcggaaag
    11281 aggcaactta tattgtaaat gactcctaaa gtcttcccca tcttgtgact gaggtatata
    11341 cctctggatt gctttttggt cagacgccgc aaaacaagaa agtaataaga tctcaatgct
    11401 tttgtcatta atataagcga agatcattct tgcaaattcg taaataaacg cccgttcctg
    11461 agcgcttaat agtttaatgc aatatttatt cttaagagag aaaataattt caaggaaagc
    11521 atccgcagca accttcacgt tattcccggc gctcatatta tatatatccc ttgcacagat
    11581 agagtacaga gatagtaaac agccatacaa ataatcattt attaactctt ttgtccattt
    11641 tactggcaac catggtttta tcttgacacc atgcatacta cagtcatgaa aaagacattt
    11701 gctgaaatta actcttatca tatcaacatt aaaaaaatgg caattaataa attttaaaga
    11761 atgagtattt acgttgttaa ttttaacaga tctaaacgaa acatttttga atacattatt
    11821 tattatattg ctatttgtta ttttaacatc acctaaactg ccgttttcaa aacaacattt
    11881 tttcaatatg ctttcattca caacagtgtg tgataagtag agattactta tgttttgctt
    11941 tattaatgcc acgtcattaa aatgactata tttaatgaaa aaaccatcat tgagtagtgt
    12001 tttaagattg actaaattac ttgcatcttc acgtaccatc gtggcaactg gaacaggcag
    12061 agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc cctttgtcat
    12121 atccacacat acgctacaaa tatcgtggta atgcgaagag cgcatttcag gaaacagatt
    12181 aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat gaacaggttt
    12241 tgtttcaaaa ttatcatcag caaatccaag cactattgta tgctctacat ctaacgcagt
    12301 ttcaaatggc tcttcttctg taaaatcatt atttaaaacc tcacctgacg tattatcact
    12361 gtcataaaaa gtgttcatca atgcgatatt atttgttaca gcgtcttttt cgatattctc
    12421 actaattgca tatcgcctgc tataatcata aaaatgaata aacgcgccct ttataaagga
    12481 tatatattgt tctttgttaa agtcaatacg gctgtataat ttttcaaaaa tcacattcgc
    12541 aattttacta ccaatggtat gcctgtataa taatgggtgc gattcaatcg taaaatgctt
    12601 gtctaattga tcttgtaatt tattctcata gtccagaggg aaaagaaact gttgtctttc
    12661 aagtttccca ttcgatacat tacgtattcc aatagagatt ctttcattac ccgcctttat
    12721 ttcaacttcg ttatcttctg atttacccag atatttttta acatcagtct cgaaagcatt
    12781 actaaaaaaa gtattaaatt ggtaattcgc tgatggcata ttttccgcga gcaacacatc
    12841 attttttatg agatcactgt cagcatttat tgctaatatc agatgcaagt agatggtatc
    12901 ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt ccctcagtga
    12961 atccaggaaa ttatcacttt gatttagttg tgtgcctgga aatgatgatg cttgatcaga
    13021 tatattgttt atcatattaa aaatcaccca agaaaaacga taatccaccg aactcctcgt
    13081 gctttataat tataaaagca caacgccttt tatattcata ttaattaacg agatactgaa
    13141 acaacttaat ttattcttcg ctaaaattca ttagcttaaa ttctttaccc acaattgtag
    13201 cataaataat attatcgaga gtcaaagctg ggactatcaa atttcgctca ttttcaaaaa
    13261 tacaacgtaa ttttggcaat catcttaatc gcaggatctc ttatcgcatg tgaaaaattt
    13321 tgtaatgatt agacaataac cgcgttcatt tatcacgcgt tataaatgtg aaactggctt
    13381 cttgcagatg ctcttaacct ctacaggagg atttatacca ccatttcaga actatcatat
    13441 ttaccttaaa tgatcatttc agccatataa cacgctcaac ctcacaaatg gtttacactg
    13501 tctattgttt gggtaattta agtaagtcag ctacgagcaa ccttgagtgg atagccagca
    13561 atggactcaa acccaatcag gtgttcagat tgtttcagaa ttgtattaaa aacaatgtat
    13621 taaataatct gatagcgttt ggtcgtagtg tgacgcagac agcaagctaa taaggaaatg
    13681 attatgcgct accgttcact atctctactt attccatgtg cactgctgct gagtgcctgt
    13741 accaccgtca cgccagctta taaagataac ggtccccgca ccggttcctg tgtccagggg
    13801 gggcctgaca gcgtggctca acaattctat gactaccgca ttcaacatcg cagcaatgac
    13861 attaccgccc tgcgccccta tttgagtgac aaactggcaa cccttcttag cgatgccagc
    13921 cgcgataata gtcatcgtga attgttaagc agcgatccgt tctccagtcg aaccaccttg
    13981 ccagatagcg cccacgttgc cagtgcatcg actatcccta atcgtgatgc ccgtaacatt
    14041 ccattgcgcg tagatctcaa acagggtgac cagggttggc aggatgaagt gttaatgatt
    14101 caggaaggcc agtgttgggt catcgacgat gtgcgctact taggcggcag cgttcatgcc
    14161 acggcgggta cgctccgtca gtccatcgag aatcgctaac gccttgatca accaataaac
    14221 cccgtaaatt gtccggcatt tcgtgaggaa tgccgacatt tatgctcgcc gaccaccgcc
    14281 cccgttattt tgtgctatgt ttattgaata atgcgctttg cttttaactt ttaaagcaga
    14341 aatattgcat aattattctg tcaaaggtac tatcttcgac ctcaatagct atcagactgc
    14401 cagtatacga gtgtcaatga gtattcaatt aaacggcatt aattgcttct acggcgcgca
    14461 tcaggcgctg ttcgatatca cgctggattg cccacagggc gaaacgctgg tgttacttgg
    14521 ccccagcggc gcgggtaaaa gctcgctgct gcgtgtactc aatctgcttg agatgccacg
    14581 ctccggtacg ctcaacattg caggcaacca tttcgatttc accaaaacac cctctgacaa
    14641 agcgattcgc gatttgcgtc gtaacgttgg catggtgttt cagcaataca acctgtggcc
    14701 gcatctgacc gtgcagcaaa acctgattga agcgccctgc cgtgtactgg ggttgagtaa
    14761 agatcaggcg ctggcccgtg cagaaaaact gctggaacgt ctgcgtctca aaccttatag
    14821 cgatcgttac ccgctgcatc tttctggtgg tcagcagcag cgtgttgcta ttgcccgtgc
    14881 gttgatgatg gaaccgcagg tactgctgtt cgatgaaccg accgccgcac tggacccgga
    14941 aattacggca caaatcgtca gcatcattcg tgagctggca gaaacgaata ttacccaggt
    15001 gatcgtcacc cacgaagttg aagtggcgcg taaaaccgcc agccgagtgg tgtatatgga
    15061 aaatggtcat atcgtagaac aaggcgacgc gagctgcttt accgagccgc aaaccgaagc
    15121 atttaaaaac tatctctctc actaagattc gggataacaa caatgaaaaa agttctgatt
    15181 gccgcgttaa ttgcaggttt tagtctttcc gccacagctg ccgaaaccat tcgttttgct
    15241 accgaagcat cctatcctcc gtttgaatcg attgatgcaa acaaccagat cgttggtttt
    15301 gacgtcgacc tggcacaagc gctgtgtaaa gagattgatg caacctgcac tttctctaac
    15361 caggcgtttg acagcctgat cccaagcctg aaattccgtc gcgtagaagc cgtgatggcg
    15421 ggcatggata tcactccgga gcgtgaaaag caggtgctgt ttaccacccc gtactatgac
    15481 aactctgccc tgtttgtggg tcagcaaggc aaatacacca gtgttgatca gctgaaaggc
    15541 aaaaaagtcg gcgtacagaa cgggacgaca caccagaaat tcattatgga taagcacccg
    15601 gaaatcacta ccgttccgta tgacagctac cagaacgcaa aactggatct gcaaaacggg
    15661 cgtatcgacg gcgtcttcgg tgacaccgca gtggtcactg agtggctgaa agataacccg
    15721 aaactggcgg cggtgggcga caaagtgacc gataaagatt acttcggcac tggcctcggc
    15781 atcgcggtac gtcagggcaa cactgagctg cagcagaaac tcaacactgc gctggaaaaa
    15841 gtgaagaaag atggcactta cgaaaccatc tacaacaaat ggttccagaa gtaattcctg
    15901 atgaatgaat tttttccttt agcaagcgcc gccgggatga ccgtcggcct tgccgtttgt
    15961 gcattgattg tcgggctggc gctggcgatg ttctttgcgg tatgggagtc ggcaaaatgg
    16021 cgtcctgtcg cgtgggcagg ttcagcgctg gtaaccattc tgcgtggcct gccagaaatt
    16081 ctggtggtgc tgtttatcta ttttggctcc tcgcagctgc tgctgacgct ttcggatggc
    16141 ttcactatca atcttgggtt cgtgcagatc ccagtgcaga tggacattga gaacttcgac
    16201 gttagcccgt tcctttgtgg tgtcatcgct ctgtcactgc tgtatgccgc ctatgcctcg
    16261 caaacgcttc ggggcgcgtt gaaagcggtg ccggtgggtc agtgggagtc tggtcaggcg
    16321 ctggggctgt cgaaatcggc tatctttttc cgtctggtga tgccgcagat gtggcgtcat
    16381 gcgctgcctg gcctcggtaa ccagtggctg gtgctgctga aagataccgc gctggtcagt
    16441 ttgattagtg tgaatgattt aatgctgcaa acaaaaagca tcgctactcg tacccaggaa
    16501 ccatttacct ggtacattgt ggcggcggcg atttacctgg tgatcaccct gctcagtcag
    16561 tacattctca aacgcattga cctgcgcgcg acacgttttg agcggaggcc cagctaatgt
    16621 ttgagtattt acccgaactg atgaaagggc tacacaccag cctgacgcta accgttgcct
    16681 cgctgattgt ggcactgatt ctggcattga tttttaccat catcctgacg ctgaaaacgc
    16741 cggtgctggt gtggctggtg cggggttata tcacgctgtt taccggtacg ccgctgctgg
    16801 tgcagatctt cctgatttat tacgggccgg gccagtttcc gacgttgcag gagtatccgg
    16861 cactgtggca tttgttgtca gaaccgtggt tatgtgcgct gattgcgtta tcgctgaaca
    16921 gtgcggcgta taccacgcag ctgttttacg gtgcaattcg tgcgatccca gaaggtcagt
    16981 ggcagtcctg tagcgccctg ggaatgagca aaaaagatac gctggcgctc ctgctgccgt
    17041 atgcctttaa acgctcgctc tcttcttatt ccaacgaagt ggtgctggtg ttcaaaagta
    17101 cctctctggc ttacaccatt acgctgatgg aagtgatggg atacagccag ttgttgtacg
    17161 gacgcaccta cgatgtaatg gtgttcggtg cggcagggat tatttacctg gtcgtcaacg
    17221 gcctgctgac gctgatgatg cgtctgatcg agcgcaaagc actggcattc gaacggcgga
    17281 attaatgtag ctgcgtacat tcgccggggg ggtttgtagg tctgataaga cgcgccagcg
    17341 tcgcatcaga catcagcacg gtgcctgtgc cggatgcggc gtggacgcct tatccggcct
    17401 acatagtgca taaattctta taatgaagac ggacaactca ctaagttatc cgtctttttt
    17461 tatttcattt aaattattta atcatattta ttgcatataa attcacttga tggcattgtt
    17521 atcccatgcc gcagacacgg ccaaaaatca taagattgac agacgggagt tccatcatga
    17581 aaaagttagt tcttgccgct ttacttgctt cctttacttt cggtgcttct gccgcagaga
    17641 aaatcaattt tggcgtttca gccacctatc caccctttga atctataggt gctaataatg
    17701 agattgtcgg ctttgatatc gatctggcaa aagccttgtg caaacaaatg caggcagaat
    17761 gtacttttac taatcacgcg ttcgacagcc tgatcccgtc actgaaattc agaaaatatg
    17821 actccgtaat ctccggtatg gatatcacgc ccgagcgtag caagcaggta tcgtttacca
    17881 cgccctacta tgaaaactca gccgtcgtga ttgccaaaaa agatacctac aaaacgtttg
    17941 ccgatctgaa aggcaaacgt attgggatgg aaaacggcac cacgcaccag aaatatattc
    18001 aggatcagca ccccgaagtg aaaactgtct cctatgacag ttatcagaat gcctttatcg
    18061 atctgaaaaa tggtcgtatt gatggggtat ttggtgacac agcggtggta aacgaatggc
    18121 tgaaaaccaa tccgcaactg ggcgttgcta ctgaaaaagt gaccgatccg caatacttcg
    18181 gcaccggtct gggcatcgct gtacgtccgg ataacaaagc tctgctggaa aaactgaata
    18241 acgcgctggc agcaattaaa gctgacggta catatcagaa aatcaatgac cagtggttcc
    18301 cgcagtaatt gagactgatg acaaaagtaa gattgcctga tgcgctgcgc ttacctggcc
    18361 cacatcaact ctgcaatgta ttgatttagc atgattttgt aggccggata aggcgtacac
    18421 gccgcatccg gcatgaacaa agcgcacctg atcagcaatc tgaagccgca cctgcggctt
    18481 ttttattgct tcaccagcag cgtcatcact tcatagtgcg cggtgtgcgg gaacatatca
    18541 aaaagctgta cccgttcgat gcggtaacct ggcagttcgc ggatatcttt cgccatggtt
    18601 tgggcgttac agctggagta gataataaaa cgcggtgcca tcgttgagag ataatcacac
    18661 agcggtttac caatgccgcg gcgcggcggg ttaaccagca ccagctccgg cacatccccc
    18721 tgagcggtgg caaactgagt ggagtccagc gcctgaaatt gcaaacgcgt taagcccagt
    18781 tcagcggctg actgctttgc acaggcaatg gcctctgatg caatttcgat cccggttaac
    18841 tgcatgtcag gcgtcgcgca gtgtaaacca aagccaccca caccgcagaa cagatcccac
    18901 atatgtttaa ctggcagctg ccgtacccag tcgcgcgcgg tagcgtacag ctggctggcg
    18961 accgccggat tagtctggaa gaaactttgc ggacggatcc acagcggtac gtcattaaaa
    19021 cgctctgcca gcgcctgttg ttcagtcagg tagatctccg tctccccttc cataatcgcc
    19081 atatgtaccg gctgaatatt gacggtaata actttcagtt gcggtagttg ttcctgtaac
    19141 cacggcagcg ccttacgcag ttgtgccagt ttggtttcag aacgcagtac aaagcgcagc
    19201 atcatgccgc catcgctctg gctttcagtc agcagaatgt atttcagttc gccacgctta
    19261 cgcgccacgt tgtagggcgt taaccccgca cgggcgataa acggttttag cgccgcaaaa
    19321 acgggcgcaa aagaggcagg ataaagcggg cagtcacaaa ggtcttccgg cgtgccatcg
    19381 cgatgcagca taccgagcag tggtttttca acgctaccac tcaccaccat tttggcttta
    19441 ttacgaaacc cttgttccgg gcctgacacc ggcgcgcacc attcctcaac cggaaaatcg
    19501 gcgagcagat ttttaagatc ggcggtttta gcggagagtt gctctggaat cggctgcgtt
    19561 atccactgac aggaacgaca gcgacccgcg tcgtaaagtg cgcactgcat acattgacct
    19621 tcacatcatc aggggcgacg attatacaca ttattgcaac tggaagaacc gccgactggt
    19681 cgagggaacg aacagcagca tcaggatgag catatccggc agcttctgca gcatcaggct
    19741 atggaagatt tcacgttttg attcaccggg aatgctgaac agctccggat aaccgtaccc
    19801 cagcgaagcc gcccacaagt aacttgcggc ggtgatttgt gtcagcagat atagccagcg
    19861 cgcccagcga cgccctttca ccagtgaaaa cgcacaccag atctcaatga aaaccagcac
    19921 cagactgctt aaaaagacca gcgttaaact ccaggtttgt acgctgcgat gaatgaactc
    19981 gccaataccg cgcacgccca gcgtattgaa aatcatcagc acgtcgaggc cacggatcat
    20041 aataatggcg agcgccgcca cctgcaccag cgcaggcaca ttcaggcgag catgagatga
    20101 agatgttttc ttaaaaaatc ccaacgtttc gtcttccatg aaaacaatgc cgcggcatgc
    20161 gcggcattat gtagccaggt tggcaaattt tagtgtcttc agccatgtct tgcacgctgg
    20221 atatcgcgta tccgctgttt ttctgcgcga gccatcagat accaggcgat aaatccgaca
    20281 attccgaccg caccgagaat taacgttgca agggcgttga tttccggatt cactcccatc
    20341 cgcacgctgg aaaagaccag catcggtaac gtggtggctc ccggcccgga aacaaagctg
    20401 gcgatcacca gatcatcaag cgacaaagta aaagccagta accagccaga aatgatcgcg
    20461 ggcatgatca tcggtagcgt aatgacgaaa aacactttca gcggcgtcgc accgagatcc
    20521 atcgctgctt cttctatcga gcgatccagt tcccgcaatc ttgaagaaat aacgaccgcc
    20581 acataagccg tacaaaaagt gacatgcgcc agccagatgg tgagcatacc gcggtccgca
    20641 ggccagccaa tggcatgagc aagcgcgacg aataataaca acagcgacaa gcccgtgatg
    20701 acatctggca tcaccagcgg cgcggtgatc ataaaggcaa aaccatttga tccgcgaaac
    20761 ctgccaaaac gcaccagcac caccgccgca atagtcccga ggatcgccgc cgccgttgcc
    20821 gcacaggcag caattgtcag gcttaaacca accgcactca tcatcgcgtc atcgcgcaat
    20881 aactcaccat accagcgcgt tgaccagccg gcccacaccg tcaccagctt cgagctgtta
    20941 aacgaataga taaccagcat cagcattggc gcgtagagaa aggtaaagcc cagcagcaaa
    21001 atcacaatcc gccagggcga acgaactacc ggtaaattat tcatccgtgt tctcccacgc
    21061 ttttttgctg gtgtttgtgg aaccacatta tcggcacaat tagcagcagc aacatgatga
    21121 tcgctaccgc cgaggccacc ggccagtcgc ggttattgaa aaactcttgc cataacacgc
    21181 gcccgatcat gatgctgtcc gggccaccga gcagttccgg gatcacaaac tcgcccacag
    21241 ccgggataaa caccagcatc gatccggcaa taatcccacc tttagtcagc ggcacgatca
    21301 cagtaaagaa cgttttcagc ggtcgtgcac cgagatccag cgctgcttcc accagcgaat
    21361 aatcaatacg aatcaacgcg gtataaatcg gcagtaccat aaacggcacg taagcgtaaa
    21421 caatgccgat ataaacggcc agattggtat gcagaatggt cagcggttga tcgataaccc
    21481 ccagccacag cagaaaatta ttcagcacac cgttgttttt taatattccc atccaggcat
    21541 aaacgcggat cagaaacgag gtccacgacg gcaggatcac cagtagtaat aaaatattac
    21601 gggtcgaagg cttactgtgc gccaccgccc acgccagcgg atagccgatc agtaaacagc
    21661 aaaatgtcga aatcgccgcc acctgtaacg actggagata agcatcaaaa tagagcggat
    21721 cgtcggtcag ttgcagaaaa ttaccgagat taagagtgat ggaaagttgc ccgtcagccc
    21781 actccatcag ttcggtataa ggtggaatag cgcgcgccat ctctcccagg cttattttaa
    21841 agacaatcag aaatggcagc agaaacagca agatcaacca gatatatagc aacgcaatga
    21901 ccagtttgcg cccatgcttc atttgcagct gcgacaacca cagcttaaat ccgcccggcg
    21961 gtttcgactg ggcagcaggt tcaagtgtac tcatctttgc tccttaaacc gtcagcacca
    22021 cacagctgtc cacttcccag cacaaacgca cttcgtcgcc ccaggtcggt aaccctttac
    22081 gatgacgatg ggcgttttgt agctgggcgc tgatcatctg cccacttttc agtcgaacgt
    22141 gatacaccga aagatcgccg agataggcaa tgtgtatcac ctcccccacc gcgaagttac
    22201 aaccattggc gggcggctct tcgcaaagca tgattttttc cgggcgcagc gctacgtgta
    22261 ccggcacgtt atcgaccact gaagcatccg catcgacttt cagtggatgc accagccccg
    22321 gcgaatcaag caccaggcca tcttcctgac gctctttgag tacaccttca aagacattaa
    22381 ccgaaccgat aaattcagcg ctgtagcggg tagttggatg ctcgtagatc tcttccggct
    22441 cgccaatctg gacaaatttc ccgcgattca taatggcgat acgccccgcc atggtcatcg
    22501 cctcttcctg atcgtgggtg accatcacac aagtcacacc gacgcgctcc agaatatcca
    22561 ccacttcaag ctgcatccgg tcacgcagct ttttatccag cgcgcccatc ggctcatcga
    22621 gcagtaatag tttcgggcgt ttggcaaggc ttcgggccag ggccacacgc tgtcgctgac
    22681 caccggaaag ctgatgcggt ttgcgtttgg cgaactcctg catgtgcacc aggccgagca
    22741 tctcatggac ccgactggca atttccgctt tcggtagttt gtcctgtttc aggccaaaag
    22801 cgatgttctg ttccacggtc atatgcggaa acagcgcgta agactgaaac atcatattga
    22861 tggggcgcag gtaaggcggc acctgtgaca aatcgacgcc atcgagcatt atctgcccgg
    22921 cagaaggttg ttcaaaacct gccagcatac gcagcagcgt ggacttgcca cagccggatg
    22981 cgcccagcag cgcgaagatt tcacctttgt agatggtcag gctgacatca tcgaccgcat
    23041 gctgaccatc gtaggatttg gtgaggttgc ggatttctaa tagcggcgtc agcgccttac
    23101 gggttttcgc ctgcgggcga gggatagcgt cattcacggc tgctctccgg cattgaatgt
    23161 gtgcaagcgc accatgatgg tgcgaattgc cggctggtgt ggtgcgcccg cccggcatct
    23221 aaggctgcgg attattttcc gctcttcact ttggtccacg cgcgggtgcg cacacggtcg
    23281 attttcggat cctgtacttt cagagtgaat agcttcgcac gaacgtccgc cggaggataa
    23341 atacccgggt tctcacggac ttccgcactc accagcggcg tggctgcttt attggcgttg
    23401 gcatagaaca catggtcgga aatatgcgct actacatccg ggcgcagcag gtaattcagg
    23461 aactgatagg cttcgtcttt gtttttggca tccgcaggca tggcgaatac atcaaagaac
    23521 gccatcgccc cttcttttgg aatcgagaac gagacattca ctccattctt cgcttccttc
    23581 gcgcggtttg acgcctgcca gacatcacct gcccagccga tagcgacgca aatatcgccg
    23641 tttgccaggt cgttaatgta ttgagatgaa tggaaataac gaatgttcgg gcgcagcttt
    23701 aacagcagat cggttgccgg tccggtgtaa tcatccgctt tagtgctgtt gggatctttg
    23761 ccgagataat tcaacacggt agcaaaaact tcttctggcg catccaggaa agagacaccg
    23821 cagcttttca gtttttccag attttcaggt ttgaggatca agtcccagct atcgacgggc
    23881 gcgttttcgc ccagcacagc tttaacttta tcaacgttat agccaatccc ggtcgtcgcc
    23941 cacatatagg gcatagcaaa tttattgtcg ggatcgtgtt tggcgaccag cttcagcagc
    24001 tccgggtcga gattcttcca ctccggcaat ttgcttttgt ccagcggctg gaaaactccc
    24061 gcagtcaact ggcgttcaag aaagctggca gatggaacca ccaagtcaaa gccggtgcta
    24121 cccgccatta atttgccttc cagtacttcg ttagagtcga aaacatcgta gacgacttta
    24181 ataccggttt ctttttcaaa attggccacc gtgtccgggg cgatataatc agaccagtta
    24241 taaatgtgga gtgttttttg ttcagccgcg agcgtgccga cagagacggc catcagagca
    24301 cccgcagcca gacccgaaag ccatttttta tttaaggcgg tcatatctct ttccttctga
    24361 aagttcgtta acaaacgaac aaaagttgtc acactaaaag atatgcaata aacgtgcata
    24421 ttttttatcg gcggggtgta agaaaagaga actctctccc ggccagcact gctcgctata
    24481 taaaagcatc gcaagaataa ctttagccag ggtttgagac tatagctcgg attaaaaaac
    24541 ggtatttaac aaatttttat gcgtatttgc tctatgtgat aaagccaaat cgacacaaga
    24601 gaagtgaaat aatctttaag aaaaggttaa aaacagcaga taattttgca gcgcgcagcc
    24661 actgcaccag cgactgcgtg ctcgaaagga gttatcagtg gaggaaatag ttttcggtaa
    24721 cgttgttttg cccttcatcg tcagtcggta acagcagttg atgggcatta gcttcaagaa
    24781 tgaccatcga aatctgctct tcgctctgac gcacgaacca tgcaaactgc tcataggtta
    24841 cgccctgcat aacggataaa gactggcaaa ccaccagctt tggcagatta tcatcctgca
    24901 tatcaagaaa tgctttcacg gtcagcgaac tggcattgat ggcagataaa tccgccgcca
    24961 gcggcaatac ggctgaaggt cggacttccg ccatggcaga aaacaggatc gtgttatcga
    25021 tcagatcgat tttggcatca aagacaccgt cgaaattctg catatggggc agatgcagag
    25081 cctgacagtt atcacattca aaaaaactca tccccaggtc atcgagccat tgacgcagcg
    25141 tatccagacc aggaacgacc agcgatgtca taattttgta caacctcttt cgataaaaag
    25201 accggcacag cttacgcaaa aagcgcaggc aaaaccatga tcagtaatgt gattgcgact
    25261 aaccacccgt tttcaggcaa tattctgtcg tagcgtggcg ttcgatccag cggatcattt
    25321 tacccgcgat gtcgatacca gtggtttttt ctattccttc cagccccggc gacgcattca
    25381 cttccatcac caacggcccg cgattagcac gcagaatatc cacaccagca acgtccagcg
    25441 ccatcgttcg cgcggctttt atcgcgattt cacgctcctg tggtgtgata ctggcgacgc
    25501 ttgccgcgcc gccacgatgc aaattggaac gaaaatcgcc ctctttcgcc cgccgttcaa
    25561 tcgcagcgac cacttcatcg ccaacaacca gacagcggat atcgcaccct tgcgcctctt
    25621 tgatatattc ctgcaccaga atatgcgcgt tcagaccgcg gaaagcgtca atcacgcttt
    25681 ccgccgcctg acgcgtctcc gccagcacga cgccaattcc ctgcgtgcct tcaaccaact
    25741 tgaccaccag cggcgcacca ccgaccatgt cgattaaatc gctggtatca tccggcgaat
    25801 gcgcaatgcc cgtgacaggc aggtcgatgc cctgacgcgc cagcagttgc atggaacgca
    25861 atttgtcacg cgcccgggca atggcgaccg actcattgag cggatagctc cccagcatct
    25921 cgaactggcg cagtgccgcc gtcccataaa aggtaatggc ggtgccaata cgcgggatca
    25981 ctgcgtcaaa atggggtaac ttgcggcctt tgtagtgaat agaagacgcc gcaggattta
    26041 tgttcatgta gcaagaaagc ggatcaagaa tttcaaccag gtgaccgcgc tgtatagcgg
    26101 cttcacgcag ccgcttacac gaatagagcg ttccatcccg ggacaatatg gcaattttca
    26161 ccctgcacct ctctgtcaga cctggtcaaa agcccgcgta tcatacatcg acgtggcggt
    26221 tttagcgcgt cgcccaaccc tgtttgtgca aataatccag aataaatggg cggctttctt
    26281 taatgattgt tcggcggata tgatcgctcc aggtatcccg gcgattattg ctgccacggg
    26341 tgaggtaata ttccgccagt tgctcgtcat actgcgccag tgcgtcttta tccagcggtt
    26401 gatagctgtt ttcatgcacc aaaatggagg ccggtaaacg cggcttgaga tccggattat
    26461 ccgcaggcca gccaaggcac agcccaaaca gcggcagaac atgctgcggt aatttcagca
    26521 gtttcgtcac cgcttcaata ttattgcgca ggccgccgat atataccccg cccaatccca
    26581 gcgattccgc tgcggttaat gcattctgcg ccatcattgc cgtatcaacg acaccgagca
    26641 acagttgttc cgccaggccg agctgagcat ccggacagat ctgtaaatgg cggttaaagt
    26701 cggcacagaa cacccagaac tccgccgctt gcgctacgtg tttttgcccg ccggtcagcg
    26761 tcaccagttc ttcacgtaat gctttgtcgg taatgcgaat aatgctacta cactgcaaaa
    26821 aactggaact ggacgtcgca cgggcgctgt taataatcgc ctcacactgc gcttcggaaa
    26881 tgggttcatc agtgaaatgg cgaatggagc gatggccaca aataagttca atggttggcg
    26941 tcattatctt tttctctttc tgaacgtgaa tattgcggtg gacggttcat cagctgtggg
    27001 gcaagacgtt ttgccacctg aagaataacc accaccgcag cgggaagcat gagcaaaaca
    27061 ccgagaaaaa tcatcagaat ctgcacttct ggccgagaaa atggctcagg cagcgacagg
    27121 gagtcgctta ccgacagcag cgccaccgcc agtagcatca ttccgataaa ttccagtatc
    27181 aacacgcctt taggcaattt accgatcgcg cgcatacgct tccctctgca aagtgagcct
    27241 tcagtctaaa acttttcact gtattgtgtt taacagttat agcttttagc aattaatgca
    27301 acaggttaaa cctactttca gcgaatacat tttagcgtga tcattacagg cataaatcta
    27361 tgaggagaga aataatgcaa accgttattt ttggtcgttc gggttgccct tactgtgtgc
    27421 gtgcaaaaga tctggctgag aaattgagca atgaacgcga tgattttcag tatcagtatg
    27481 tagatattcg tgcggaaggg atcactaaag aagatctaca acaaaaggca ggtaaacccg
    27541 tagaaaccgt gccgcagatt tttgtcgatc agcaacatat cggcggctat accgattttg
    27601 ctgcatgggt gaaagaaaat ctggacgcct gatcgtctga caagccctcg cgttgagggc
    27661 tttactgatt ttttctgtgc tgtggtttaa acaaactact gataaataag aaacacagtg
    27721 cccccagcgc acaccagaac accgcgctta gtaaccatgc cagctcttgc cagaatgagc
    27781 gcgtcggtga aaaaaacagc cgcataatga gcattgaaca gggtgccgcc agcattgcgc
    27841 caaatagagg tttcaggact tctctacgct gtgaaaagaa actggcaact gctccaggaa
    27901 gaatgaaaaa cagcaagccg atttcaggat gcccggcagc ccgaaaagcg cctttcatgt
    27961 gcgtcgccag aaaaaggcac accacaatga agaggacaaa acagcagatt gcccccgccc
    28021 aacgttgttt atgtttcact cgttcctcct gacactgcgt ctatcgaaca catttttcgc
    28081 cagtgtggcg ttcagtaaga taaagccgct tcgcattcca tgctaatata ggccaacgca
    28141 attcatatag ccgttgatac ctaatgtgat tacactagta aaatatattg ttactttact
    28201 atcgtttagg tgcgctgaat gaatctgcgc cctgaattct ggtaaaaaac attatcgtaa
    28261 attaccattt ctttcaacag cttactagta aacaagaagt tagcctccgt gaatataaac
    28321 gtcgccgaat tgttaaatgg gaattacatt ctgttattat ttgtggtcct cgcgcttggg
    28381 ctatgtctcg gaaagttacg acttggttcg atccaactgg gtaattccat tggcgtttta
    28441 gtcgtatcgc tgttattagg ccaacaacat ttcagcatta acaccgatgc gcttaatctt
    28501 ggctttatgc tgtttatttt ctgcgtcggg gtcgaagccg gaccgaactt tttttccatt
    28561 ttttttcgcg atgggaaaaa ttacctaatg ttagcactgg tgatggttgg cagtgcgctg
    28621 gtgatcgcct tagggttagg taagctgttt ggctgggata ttggcctgac ggccggtatg
    28681 ttagcaggct ctatgacgtc gacaccggtt ctggtcggtg ctggcgatac actgcgtcat
    28741 tccggcatgg aaagcaggca gctctcactg gcactggata atctgagcct cgggtatgcc
    28801 ttaacctatt taatcggtct ggtgagtttg attgttggtg cgcgttactt gccgaaattg
    28861 cagcatcagg acttacagac cagcgcccag caaatcgccc gcgaacgtgg cctggacact
    28921 gatgccaacc gtaaggttta tttaccggtg atccgcgcct atcgcgtcgg cccggagctg
    28981 gtggcctgga ccgacggcaa aaatctgcgt gaactgggta tttatcgaca aaccggctgc
    29041 tacattgaac gtattcgacg taacgggatt ctggcaaatc cagacggtga tgccgtgcta
    29101 caaatgggcg atgaaatagc gttggtaggc tatcccgacg cccatgcccg actcgatccc
    29161 agcttccgta acggtaaaga agttttcgat cgtgaccttc tcgacatgcg tatcgtcact
    29221 gaagaagtgg tcgttaaaaa ccataacgct gtaggtaaac gtctcgcaca actgaagttg
    29281 accgatcacg gttgcttcct taaccgcgtc attcgtagcc agattgagat gccgatagat
    29341 gacaacgtcg tgcttaacaa aggtgacgtt ttacaagtca gcggtgatgc ccgtcgcgta
    29401 aaaaccatcg ccgatcgcat cggctttatc tcgattcaca gccaggtcac tgacctgctg
    29461 gcattttgcg ccttctttgt tattgggctg atgatcggga tgatcacctt ccagttcagc
    29521 acattcagtt tcggcatggg gaacgctgcc gggttgttat tcgccggaat tatgctgggc
    29581 tttatgcgtg ctaaccaccc gaccttcggt tacattccgc aaggtgcatt aagcatggtg
    29641 aaagagttcg gcttgatggt gtttatggca ggcgttggtc tgagcgccgg tagcggtatt
    29701 aataacggcc tgggcgcgat tggcggtcag atgttgattg ccggattaat tgtcagtctt
    29761 gtgcccgtgg ttatctgttt cttgttcggt gcttatgtat tgcgaatgaa ccgcgcactg
    29821 ttgttcggcg caatgatggg cgcacgcacc tgcgcgccgg caatggagat catcagtgat
    29881 acagctcgca gtaacatccc tgcgctgggc tatgcgggca cctacgcaat cgccaacgtc
    29941 ctgctgacgc tggcagggac aatcatcgtc atggtatggc caggattagg ataaaactga
    30001 agttgccctg aaaatgaaat ttttttgcac aaccgcagaa cttttccgca gggcatcagt
    30061 cttaattagt gccactgctt ttctttgatg tccccatttt gtggagccca tcaaccccgc
    30121 catttcggtt caaggttgat gggttttttg ttatctaaaa tttatctact accctgcaac
    30181 cctctcaacc atcctcaaaa tctcctcgcg cgatagcggc ttacggtcgg tgacaaaatg
    30241 cagcgtcatc ccctcaataa acgcatcaag cgcgcgggcg gttccgggtt caaaccattg
    30301 ttcgagcgtt tgctgactgc gctgcatcca gttttgcatt accgttttta atagcggttt
    30361 tcggctagcc agcgcgtaga gctggtacat cagctccata ttatccggcg ttgcaacctg
    30421 tgagctgtag atcatatcgg tgatagcctg gcatgcgcct tgagcatcac taacatcgct
    30481 aaaaaatgcc tgatattgcc gggacataat ctcagtaaaa ctgctgaacg cctccagcaa
    30541 caactcatca attcctgaaa aatagtaggt catcgatccc aacggtaccc cggcaagggt
    30601 agcaattttg cggtgcgtaa cagcatgtat tccgtaaagt ttcaccgcct ccagcgtggc
    30661 ctggataatt ttttctcgcc gttgcggatc gttagcgcga cgcatgtcat ttcctctgtc
    30721 tgcaattgtg tacaaatgta cacaaccttg ctaatgttgt cttccctctt cttattttga
    30781 cgtgatctat gaccgtaaat tcttcacgta atgcattgaa acgccgaacc tgggcgctgt
    30841 ttatgttctt ctttttgcca ggcctgttaa tggcgtcctg ggcaacccgt acgcctgcta
    30901 tccgcgatat tctctctgtc tcgatcgctg aaatgggcgg tgttctcttt ggtctgtcga
    30961 tcggttcgat gagcggtatt ctctgctcgg cgtggttagt gaaacgcttt gggacacgta
    31021 atgtcatcct ggtcacgatg tcctgcgcat tgatcgggat gatgatatta agtctggcac
    31081 tctggctgac atcgcccctg ctctttgccg ttggtctcgg cgtctttggg gcaagttttg
    31141 gttctgcgga agtggcgata aacgttgaag gtgccgccgt tgagcgagaa atgaataaaa
    31201 cggttttgcc gatgatgcac ggtttttata gcctaggcac gctggcaggc gctggtgtcg
    31261 ggatggcact gacggccttt ggcgttccgg caacggtgca cattttattg gcggcgctgg
    31321 taggtatcgc gcctatttat atcgccattc aggcaatccc tgacggtacg ggcaaaaatg
    31381 ctgccgatgg cacccagcat ggcgaaaaag gcattccttt ttatcgcgat atccagttgc
    31441 tgttgattgg tgttgtagtg ctggcgatgg cctttgccga aggttctgcc aacgactggt
    31501 tacccttatt aatggttgac ggtcacggtt ttagccctac ttccggctcg ctgatttatg
    31561 ccggttttac cctagggatg accgttggac gcttcactgg cggttggttc atcgaccgtt
    31621 acagtcgcgt tgccgtggtt cgggccagtg cactaatggg ggcgttgggt attgggctga
    31681 ttatttttgt cgatagcgcc tgggtcgctg gggtgtctgt tgtactctgg ggactgggtg
    31741 cctcgctggg cttcccgctg accatttctg ccgccagcga taccggcccc gatgcaccaa
    31801 cccgcgtcag tgtggtagca acgaccggtt atctggcttt cctcgtcggg ccgccgctgc
    31861 tgggctatct cggcgaacat tatggattac gtagtgcaat gctggttgta ctggcgctgg
    31921 ttattctcgc ggctattgtc gcgaaagccg tcgccaaacc cgataccaaa acgcggacgg
    31981 cgatggagaa tagttgatga gcattaaatt aattgcggta gacatggatg gtactttctt
    32041 aagcgatcaa aaaacctata accgtgagcg gtttatggct cagtatcagc aaatgaaagc
    32101 acaaggcatt cgctttgtgg tcgccagcgg gaatcaatat tatcagttgc tctctttctt
    32161 ccctgaaatt gctaatgaaa ttgcctttgt ggctgaaaac ggcggctggg tagtgagtga
    32221 aggcaaagat gtttttaatg gcgagctgtc gaaggatgcc tttactactg tcgtggaaca
    32281 tttgctgacg cgcccggaag tggaaattat tgcctgcgga aaaaatagcg cctatacgct
    32341 caaaaaatat gacgatgcca tgaaaacggt ggcagaaatg tattatcacc gtctggaata
    32401 cgtcgataac tttgacaact tagaagatat tttctttaag tttggcctga atctttccga
    32461 tgaactgatt ccacaagtac aaaaagcatt acatgaggcc atcggcgata ttatggtgcc
    32521 agtccacacc ggcaacggca gcatcgatct gattatcccc ggcgtacata aagccaatgg
    32581 ccttcgccaa ctgcagaaat tatggggaat agacgacagc gaagtggtgg tctttggcga
    32641 tggcggtaac gatattgaga tgctgcgtca ggcaggcttt agttttgcaa tggaaaatgc
    32701 cggcagcgcg gtcgtcgcag cggcaaaata ccgggcaggc tccaataacc gtgaaggcgt
    32761 actggatgtg atcgataaag tgcttaaaca cgaagcgcca tttgaccaat aaaaacggca
    32821 gcacgcccgg caacacaata cctgaatgaa ggctgcataa ttcgcggaaa ttaacccgct
    32881 gcattatttc aggcgaaatg attatgaaaa attgtctact gttgggcgcg cttttaatgg
    32941 gctttactgg tgtggcgatg gcgcaaagtg tcaccggcga tgtgccgagt ggatacaaag
    33001 tggttgtagt tcctgattca gtcagcgttc cacaagcggt cagcgtcgcg actgtgccgc
    33061 aaaccgtcta tgtggctcct gcgccagctc cggcttaccg cccgcatcct tatgctcgcc
    33121 atcttgccag cgtaggtgaa gggatggtta tcgaacacca gattgacgac catcaccatt
    33181 aattcgagaa tgcctgatcg cacaaagcag tcaggcattt ttttaccctt cgtgagaatt
    33241 tcccatctgt ttatctttta aaaagataac catcagcgac agccacaaaa ttccgttgac
    33301 aaggttgaag agattaaact gtccgttgcc cccgttcagc caggcatgtt tgctgatttc
    33361 aataccaacg gtaaagatca gcatttgcag cattcccatc gccgcagaaa ccgtaccttt
    33421 actcatatcg ctggcaaaca gggttaatcg caccagtccc gcattcgcca gaccaatacc
    33481 gaaagcataa atacttaacc cggcagtcat ccataaatac gcgtgcgatg agataaccgt
    33541 tgccgcagca gcgaccaaca gaccaatcat aatcggccag ccgcccataa ttatcacgta
    33601 cggtgcggcg cgaggtcaga cgcgctaaca gcaagttacc cgcaattaac gccccgaaaa
    33661 taggcacttg cagcaagcca tattcatagc tgctcaactg ctccccggta atgatgataa
    33721 tcggcgactg ggcgatccac gccagcaatg gcaggctaac gaatcccagc gccagcgccc
    33781 ccgccacaaa gcggccgttc ttcagcacca gcttatagtc acgaccgagt tctttcagtg
    33841 acagtttctc gcctatacgc gtggcggttt caggcatagc tcgttgcaga ccgaaaaagg
    33901 agatcgctgc caatgcggca aacaaaacaa acatcccctc ccagggcagc acatggatcc
    33961 acgccgcgcc caccagcgga ccaagtagcg gagcaatcag cgccacgttc gccatcagcg
    34021 cggtgatctt gatacaaacc gcctcttcga aggattcctg aattgcggcg tatcccacag
    34081 cgccaatgaa acagaggctt atgccctgca agaagcgcaa cagggtgaat tgttcaatgt
    34141 tttgcgccag taatattgcc agacaggtga cgataaacca caccacaccc gccagcatca
    34201 ccggacggcg accaatacga tccgacagcg gccccaggag ccattgtaaa aacatcccgc
    34261 ccgccagata cgcggtcatc gaagtaggaa cccaatcaat gcccgcctga tattgttcca
    34321 ccacggccaa cataccgggt tggatcatat cgttgccgat ataggttgaa aattcgtaaa
    34381 gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg aaagctaatt
    34441 tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa aagcgcagct
    34501 aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac attattgcga
    34561 gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc acccaccgta
    34621 aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc tgcactttat
    34681 tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga agttatgctg
    34741 gaaaatttga atctctctct attctctctt attaacgcga cgccagactc ggctccgtgg
    34801 atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc gttgctggcc
    34861 gcggtacttt ggttgtgggg gcttacagca caacggcaac tggtgataaa aatcgctatc
    34921 gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc gcacgaccga
    34981 ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga ctcattcccg
    35041 agcgatcacg gtacggtgat tttcaccttt gcactggcat ttttatgctg gcatcgcctg
    35101 tggtccggct cacttttaat ggttctggcc gtcgtcattg cctggtcgcg cgtttatctt
    35161 ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat tggctgcctt
    35221 agtgcccaga ttatctggca agcgatgggg cataaactct atcaacgtct gcaatcgtgg
    35281 tatcgcgtct gttttgcatt accgatccgc aaaggctggg tgcgtgactg atctctgcta
    35341 aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat ggaaacacgt
    35401 cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa gttacatctt
    35461 aaagacgccg ccgccctgct tggggtttcg gagatgacga ttcgtcgcga tctgaacaac
    35521 cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgcg cagtgccagc
    35581 cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg ggcggcaaaa
    35641 ctggctgcga cactggtaga acccgatcag accctctttt ttgactgtgg caccaccacg
    35701 ccgtggatta ttgaagcgat tgataatgaa atccctttta ccgccgtttg ttattcgcta
    35761 aatacctttc tggcgctgaa agagaaaccc cattgccgcg catttctgtg cggtggtgaa
    35821 tttcacgcca gcaacgccat tttcaacccc atcgattttc agcaaacgtt gaataatttt
    35881 tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg cgctacctgt
    35941 tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca aaagcatgtg
    36001 ctggttgtcg accacagtaa atttggcaag gtgcgtccgg cgcgcatggg tgacctgaaa
    36061 cgctttgata ttgtggtgag tgattgttgc ccggaagatg agtatgtgaa gtacgcgcag
    36121 acgcaccgta ttaagttgat gtattaatga cgtataaccg gatgacgttt cgcgccatcc
    36181 ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc atcatcacga
    36241 aatcccacat ccgaccaaag aatccgccct cttccacatt ttccatcacg atcagcggac
    36301 gctgctcaat ggatttaccg ttaagctgga aatcaatggt cccgacaacc tgaccttttt
    36361 tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg tttttcagct
    36421 gcccacgcgg tatggtcacg gagcccgctt cgcctgcccc gagattcact tcgctcttat
    36481 caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc gtcacagttt
    36541 caaagaagcg gaaaccccag gtcaataatt tctcagactc attaaaacgg atacggtcag
    36601 ttttcgcccc cagcactacg gagattaaac gcatatcacc ctgggtagcc gaagcaacca
    36661 gattatatcc cgcacctgcc gtggttcctg tcttcatgcc atcaacattc agattgctgc
    36721 tccacagcag acggttacgg ttaggctgac gaattttgtt gaaggtgaac tctttctctt
    36781 tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc tttacccagc aatgccatat
    36841 cgcgcgcggt actgaactgc cccggcgcat ccagaccgtg caccgtctgg aaggtagtgt
    36901 tggtcagacc cagttttttg gcataaccat tcatcaaacc aataaatgac tcctggctcc
    36961 cggcaacgta atcggccagc gcaatacagg cgtcattacc ggactggata atcacacctt
    37021 tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag gaacattacc gatgaaccac
    37081 gcagtgccgg atttcccgtc gcccaggcat ctttaccgac cgtcaccata tcggtgagtt
    37141 taatcttatc ggccttaagc gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc
    37201 tcgcgggatc cagtttctca tccgcgttgc cttctgccag cactttaccg ctggcgtaat
    37261 ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc aacggtttgt tccgccgcga
    37321 atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc tgcaagacca cgaaggagag
    37381 aggagtattg cgtcataaat gccacccgag tatccattca aaaaaaatac gttatatcgc
    37441 cgtatgataa gttcggtgat taataacgca cacacaaact taaagaaacc gtaagttggt
    37501 aaagttttta aagtttatgc aatatcctgc cgcttcgctg cttctggtgt catttttttg
    37561 ccagctgttg cttaaaagtt aactttactt caccatcgga gtcctgttca gcctgtggct
    37621 gcctgtcgga aaaggggtga gtatgattac gctgtggggt cggaataatt caactaacgt
    37681 aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat tctcgcgggc
    37741 cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa cgggctggtg
    37801 ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc cattgtccgc
    37861 tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg
    37921 gaagcggaaa aatggatgga ctgggcaaac cagacgctca gtaatgctca tcgcgggatc
    37981 ctgatgggat tagtcagaac accaccggaa gagcgcgatc aggccgccat tgatgccagt
    38041 tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg
    38101 ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat ctacaatttg
    38161 tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta tcagcaactc
    38221 actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt gcgtgggcta
    38281 actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca
    38341 gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc ttctgtcact
    38401 ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc aataaataat
    38461 ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct cacagcgggc
    38521 gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc tttcgcttcc
    38581 ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc gtagtttttg
    38641 cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt cagccacagt
    38701 gcattactcc acggattcat cgccattcct tgcggattac gaatgccata agaccagatc
    38761 tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc gccctggtcg
    38821 gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg gcgctgattg
    38881 ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg cccgccaaaa
    38941 tggttgccgg tagacagttt tggcatctgg cggaagacgg tgcggaagtc ggtcactttt
    39001 gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc atcatcgcca
    39061 acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc taaaaccacg
    39121 tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat
    39181 aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat taacataccg
    39241 tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg cagtacttcg
    39301 acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag gggcacaagg
    39361 aaaaaggatt gtcgatgcat aacgcctccg gattgactct ggcttaaagc gtagtcagtg
    39421 gaggagataa caaattcatt tttacaaaaa cttaaacatg aagggggaga cgctttctcc
    39481 cccttagttt tcaggccttc tcaagcatgg cgtgcttctg caggctctgg atactcagcg
    39541 ttaagctcat cagacaattt tcaagcttat cggcgttgac ggtaataaca gtcgggcaat
    39601 catggtgccc actcatcaaa catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa
    39661 gagcactcca ctcttcctga tccagatgaa gattcaaccg cagcgattta tcgtgcagtt
    39721 cgcgattcag tttaaaaaag ttatctcgta gatgattgct ttcgctgacg gacatgtatc
    39781 cttttgcctt cctcagttgc acgtatgcag cgaggtcaac gcgtgcgtcg atcaaccggt
    39841 tcagcagatc gattcgctgt ctgtcaacga acatacttcg atcctcctct ttccagcctt
    39901 gttgcgatta aatagtatgg ctattttttg agcaaaatgc tgtccaggat caattcttca
    39961 cgccaaatta acaaaattta catttccttc gcctccgcca gcgaatgaga ttgcgataca
    40021 aattttatcc agaactctgt agaatccctg ccctgacctt tcctgtaact gaatattttt
    40081 taagctatga gcaaagtaac tccccagccg aaaatcggct ttgtttccct tggctgtccg
    40141 aaaaaccttg tcgattcaga gcgtattctc accgaactac gcactgaagg ttatgacgtg
    40201 gtaccgagct atgacgatgc ggacatggtg atcgtcaaca cctgcggctt tattgacagc
    40261 gcggtacaag aatcactgga agccattggt gaagcgttga atgaaaacgg caaggtaatt
    40321 gtgaccggtt gtctgggggc aaaagaagat cagatccgcg aagtccaccc gaaagtgctg
    40381 gaaatcaccg ggcctcatag ctatgagcag gttctggagc acgttcatca ctacgtgcca
    40441 aaaccgaaac acaacccatt cctgagcctg gtgccagaac aaggtgtgaa actgacaccg
    40501 cgtcattatg cctatctgaa aatttcggaa ggctgtaatc accgctgcac cttctgcatt
    40561 attccgtcta tgcgcggcga cctggtgagc cgtccgattg gcgaagtgtt aagtgaagcg
    40621 aaacgtctgg tagatgcggg cgttaaagag attctggtga tctcgcagga tacttccgcc
    40681 tatggcgttg atgttaaaca tcgtactggc ttccacaacg gcgagccggt aaaaaccagc
    40741 atggtcagcc tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct gcactacgtt
    40801 tacccttatc cgcatgtgga cgacgtcatc ccactgatgg cagaaggcaa aatcctgccg
    40861 tatctggaca ttccgttgca gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg
    40921 ggttctgtag atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg
    40981 accctacgct caacctttat tgtcggcttc cctagcgaga cggaagaaga tttccagatg
    41041 ctactcgact tcctgaaaga agcgcgtctg gatcgcgttg gctgctttaa atacagcccg
    41101 gttgaaggtg cagacgccaa tgccctgcct gaccaggttc cggaagaagt gaaagaagaa
    41161 cgctggaacc gtttcatgca gctgcagcag cagatttccg ccgagcgcct gcaagagaaa
    41221 gtgggccgtg aaattctggt gattatcgac gaagtggacg aagaaggcgc gattggtcgc
    41281 agcatggcag atgcaccgga aatcgacggc gcggtttatc tcaatggtga aaccaacgtt
    41341 aagccgggtg atatcctgcg tgtgaaagtc gagcacgccg atgagtacga tttgtggggt
    41401 agccgggttt aattaattca ggccgggcaa aatcatttgt ccgccctgcg aataatgatt
    41461 actgccagct aatctgagcc ctgccgtttt tcttactctt atacagccgc tcatccgcca
    41521 gcattttcaa gcgttcgacg gtggtacatt catttgaatg cgctacaccg atactggtac
    41581 ttaaattaat gcttcccacc tggaaatgaa cgatttcttt tagaatcgat agcaactctt
    41641 ccagcgttcc acccgtataa atcaccgcaa attcgtcacc gccgaagcgg tagattttac
    41701 ctttatctac cgggatctgt gatttaagga ttttcaccac tttcatcaac acttcatcgc
    41761 ccagcaaatg accgaaggtg tcgttaatgt ttttgaattt atcgacgtca aacaggcaca
    41821 aataaccgcc tttttccggc agcgctttta aatcttcgtc aaaggatttt ctgcccattg
    41881 ctttggtcag gtcgtccagg aacgccgagt tatacatact gtagagtttt ttgacatcgg
    41941 cagccgaaaa gaaaagcccg ccatccagta cgcgttcaaa atggttatta atgatggctt
    42001 ctttctccac aataccacga ttaatcactc tgcgtaccag atagaggcaa ctggtacccg
    42061 atatcactgc caccaatgcg gcaaagaaaa taatcaatgt acagacccgc aaataaacat
    42121 cttttttata cgcaaatacg tacaatccgg tcggcaaata ggcgaagtgc tccatcgctt
    42181 ccgaaacgat gaagatggcg tttttagcat tatattcatc accggtttac cgttcttaaa
    42241 tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta aagaaaaacg
    42301 ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga ggtaatagac
    42361 gaaggtttta ccgtcaaaag agataaaccc tgtatttttg acatgtcgat acttcacaac
    42421 ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac attcgtcact
    42481 ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt cggcgtaagc
    42541 tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga taagatcaat
    42601 tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat caataacgga
    42661 ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc aggcaactgc
    42721 tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga ttctggacat
    42781 acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat ggcatgggtt
    42841 tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg cgcgcaacgt
    42901 cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa
    42961 taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc gagtccacct
    43021 ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt
    43081 catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga taaaaatgtt
    43141 cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc gccttcaggc
    43201 cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt aactgccagg
    43261 gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgctga ctaaaatgtc
    43321 tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata aacccgatat
    43381 cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca gaaacccaac
    43441 ggcacaacgc ctccgcgccc agaatggcgc gggtgttaat atcaacgatc ggttgataga
    43501 cgaggaatag ttcgccaatt tccattgcat ggcgaatatt acgcgcaatc cagatattct
    43561 cttctatttc gttgagtttc gcgtcatcac agaaaataaa ctcggagaac atatggtcac
    43621 ggatactgga aatcgccagt ttcagttttt ctgcgtaact ccaggcgttt tccccctgca
    43681 aaggttcaca aatggcagac ttatggatgc gcagattgcg gtagccaaag ctgctgtcaa
    43741 aatcgtttac catcgctatt ttacgggtaa atgtctgtac attttcccga cacagaagta
    43801 aatagagatc ggcattgaat gtcacgatat cgcagcattg cgcatatttc tcggcaattc
    43861 gctgacacaa tttcctgacg attttattgc gttcatcaac accaaacacc gcttccacgg
    43921 cttccagacc acacagtttg atgatcccca cagccagata ctgattattg ttggactcaa
    43981 taatcgccat atttttaata aaaccgcttt cctgatcgta agagatcagt gagagcaatt
    44041 tatttgaccg ttcaatttcg gcatctttca tttccgcgat cg
//
LOCUS       DAQSWQ010000024        43399 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.024, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000024 DAQSWQ010000000
VERSION     DAQSWQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.024"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(2..94)
                     /locus_tag="R8G18_001370"
     tRNA            complement(2..94)
                     /locus_tag="R8G18_001370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="R8G18_001371"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="R8G18_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEE9795119.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="R8G18_001372"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="R8G18_001372"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEE9795120.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="R8G18_001373"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="R8G18_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEE9795121.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4316..5377)
                     /gene="recA"
                     /locus_tag="R8G18_001374"
     CDS             complement(4316..5377)
                     /gene="recA"
                     /locus_tag="R8G18_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEE9795122.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="R8G18_001375"
     CDS             complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="R8G18_001375"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HEE9795123.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="R8G18_001376"
     CDS             complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="R8G18_001376"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEE9795124.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7440..8003
                     /gene="srlA"
                     /locus_tag="R8G18_001377"
                     /gene_synonym="gutA"
     CDS             7440..8003
                     /gene="srlA"
                     /locus_tag="R8G18_001377"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEE9795125.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8000..8959
                     /gene="srlE"
                     /locus_tag="R8G18_001378"
                     /gene_synonym="gutE"
     CDS             8000..8959
                     /gene="srlE"
                     /locus_tag="R8G18_001378"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEE9795126.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            8970..9341
                     /gene="srlB"
                     /locus_tag="R8G18_001379"
                     /gene_synonym="gutB"
     CDS             8970..9341
                     /gene="srlB"
                     /locus_tag="R8G18_001379"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEE9795127.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9345..10113
                     /gene="srlD"
                     /locus_tag="R8G18_001380"
                     /pseudo
     CDS             9345..10113
                     /gene="srlD"
                     /locus_tag="R8G18_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10219..10578
                     /gene="gutM"
                     /locus_tag="R8G18_001381"
     CDS             10219..10578
                     /gene="gutM"
                     /locus_tag="R8G18_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HEE9795128.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10645..11418
                     /gene="srlR"
                     /locus_tag="R8G18_001382"
                     /gene_synonym="gutR"
     CDS             10645..11418
                     /gene="srlR"
                     /locus_tag="R8G18_001382"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HEE9795129.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11411..12376
                     /gene="gutQ"
                     /locus_tag="R8G18_001383"
     CDS             11411..12376
                     /gene="gutQ"
                     /locus_tag="R8G18_001383"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HEE9795130.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12373..13887)
                     /gene="norR"
                     /locus_tag="R8G18_001384"
     CDS             complement(12373..13887)
                     /gene="norR"
                     /locus_tag="R8G18_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HEE9795131.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14074..15513
                     /gene="norV"
                     /locus_tag="R8G18_001385"
     CDS             14074..15513
                     /gene="norV"
                     /locus_tag="R8G18_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HEE9795132.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15510..16643
                     /gene="norW"
                     /locus_tag="R8G18_001386"
     CDS             15510..16643
                     /gene="norW"
                     /locus_tag="R8G18_001386"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HEE9795133.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16771..19023)
                     /gene="hypF"
                     /locus_tag="R8G18_001387"
     CDS             complement(16771..19023)
                     /gene="hypF"
                     /locus_tag="R8G18_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEE9795134.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19176..19703)
                     /gene="hydN"
                     /locus_tag="R8G18_001388"
     CDS             complement(19176..19703)
                     /gene="hydN"
                     /locus_tag="R8G18_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HEE9795135.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19851..20861)
                     /gene="ascG"
                     /locus_tag="R8G18_001389"
     CDS             complement(19851..20861)
                     /gene="ascG"
                     /locus_tag="R8G18_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HEE9795136.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21121..22578
                     /gene="ascF"
                     /locus_tag="R8G18_001390"
     CDS             21121..22578
                     /gene="ascF"
                     /locus_tag="R8G18_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HEE9795137.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22587..24011
                     /gene="ascB"
                     /locus_tag="R8G18_001391"
     CDS             22587..24011
                     /gene="ascB"
                     /locus_tag="R8G18_001391"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HEE9795138.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24125..24544)
                     /locus_tag="R8G18_001392"
     CDS             complement(24125..24544)
                     /locus_tag="R8G18_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEE9795139.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24541..24852)
                     /locus_tag="R8G18_001393"
     CDS             complement(24541..24852)
                     /locus_tag="R8G18_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEE9795140.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25014..25787)
                     /locus_tag="R8G18_001394"
     CDS             complement(25014..25787)
                     /locus_tag="R8G18_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795141.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25812..26282)
                     /gene="hycI"
                     /locus_tag="R8G18_001395"
     CDS             complement(25812..26282)
                     /gene="hycI"
                     /locus_tag="R8G18_001395"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HEE9795142.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26275..26685)
                     /gene="hycH"
                     /locus_tag="R8G18_001396"
     CDS             complement(26275..26685)
                     /gene="hycH"
                     /locus_tag="R8G18_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HEE9795143.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26682..27449)
                     /gene="hycG"
                     /locus_tag="R8G18_001397"
     CDS             complement(26682..27449)
                     /gene="hycG"
                     /locus_tag="R8G18_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HEE9795144.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGMDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27449..27991)
                     /gene="hycF"
                     /locus_tag="R8G18_001398"
     CDS             complement(27449..27991)
                     /gene="hycF"
                     /locus_tag="R8G18_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HEE9795145.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28001..29698)
                     /gene="hycE"
                     /locus_tag="R8G18_001399"
     CDS             complement(28001..29698)
                     /gene="hycE"
                     /locus_tag="R8G18_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HEE9795146.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29716..30639)
                     /gene="hycD"
                     /locus_tag="R8G18_001400"
     CDS             complement(29716..30639)
                     /gene="hycD"
                     /locus_tag="R8G18_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HEE9795147.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30642..32468)
                     /gene="hycC"
                     /locus_tag="R8G18_001401"
     CDS             complement(30642..32468)
                     /gene="hycC"
                     /locus_tag="R8G18_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HEE9795148.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32465..33076)
                     /gene="hycB"
                     /locus_tag="R8G18_001402"
     CDS             complement(32465..33076)
                     /gene="hycB"
                     /locus_tag="R8G18_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HEE9795149.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33201..33662)
                     /gene="hycA"
                     /locus_tag="R8G18_001403"
     CDS             complement(33201..33662)
                     /gene="hycA"
                     /locus_tag="R8G18_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HEE9795150.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33874..34224
                     /gene="hypA"
                     /locus_tag="R8G18_001404"
     CDS             33874..34224
                     /gene="hypA"
                     /locus_tag="R8G18_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEE9795151.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34228..35100
                     /gene="hypB"
                     /locus_tag="R8G18_001405"
     CDS             34228..35100
                     /gene="hypB"
                     /locus_tag="R8G18_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEE9795152.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35091..35363
                     /gene="hypC"
                     /locus_tag="R8G18_001406"
     CDS             35091..35363
                     /gene="hypC"
                     /locus_tag="R8G18_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HEE9795153.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35363..36484
                     /gene="hypD"
                     /locus_tag="R8G18_001407"
     CDS             35363..36484
                     /gene="hypD"
                     /locus_tag="R8G18_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEE9795154.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36481..37491
                     /gene="hypE"
                     /locus_tag="R8G18_001408"
     CDS             36481..37491
                     /gene="hypE"
                     /locus_tag="R8G18_001408"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HEE9795155.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37565..39642
                     /gene="flhA"
                     /locus_tag="R8G18_001409"
                     /pseudo
     CDS             37565..39642
                     /gene="flhA"
                     /locus_tag="R8G18_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39679..40032)
                     /locus_tag="R8G18_001410"
     CDS             complement(39679..40032)
                     /locus_tag="R8G18_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HEE9795156.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40109..40243)
                     /locus_tag="R8G18_001411"
     CDS             complement(40109..40243)
                     /locus_tag="R8G18_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795157.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40319..42880
                     /gene="mutS"
                     /locus_tag="R8G18_001412"
     CDS             40319..42880
                     /gene="mutS"
                     /locus_tag="R8G18_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEE9795158.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVTNVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42986..43399
                     /locus_tag="R8G18_001413"
                     /pseudo
     CDS             42986..43399
                     /locus_tag="R8G18_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10276 a        11917 c        11302 g         9904 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatccct acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gagtgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcacca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtgtgcagc caattcgtta gaggatcgag
     4201 gtgagagtaa tttttcacgc cagtgttggc ctgttcccct caccctaacc ctctccccaa
     4261 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4321 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4381 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4441 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4501 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4561 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4621 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4681 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4741 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4801 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4861 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4921 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     4981 gggcgtcaca gatttccagt gcctgctcgc cggtgtccgg ctgggagcac agcaggttat
     5041 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5101 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5161 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5221 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5281 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5341 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5401 ataccggata gtcaatatgt tctgttgaag caattatact gtatactcat acagtatcaa
     5461 gtgttttgta gaaattgttg ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc
     5521 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5581 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5641 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5701 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5761 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5821 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5881 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     5941 tcactgtcag tcatcacaac ctcagtcagt caaaaattta tgccggacaa gatagcactt
     6001 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6061 tagccagagg gaagctcacg cccccctctt gcaaatagtt actgcacgcg cgccagcgcc
     6121 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt ataacgggta
     6181 atggtgtaga agttcggcag accgtaccag tactggtaac cggtaccgac atccagacgc
     6241 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgccgtggca
     6301 agctgcgaaa tgctgtacct ggttttgaag ccatttggca agcctggagc ctgaccgttt
     6361 gccattaccg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccaca
     6421 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6481 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6541 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcaacaggaa ggtttccagc
     6601 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6661 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6721 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6781 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6841 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6901 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     6961 cgatcgaaac cgtgtttgtt caccattctg tcgatgaact gctgggcatt cgggttatta
     7021 gcgaaatcgc cgcccatctg catcacattg tgctgaggct caagcaggaa gccgccagac
     7081 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7141 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7201 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7261 ctgcccgata cggactttac ataactcaac tcattcccct cgctatcctt ttattcaaac
     7321 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaatctttca
     7381 atctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7441 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7501 aggtgtttac cgggatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7561 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7621 ccggtaaccc tgtttcccgt tacctgctgc taccgtgcat tggcacgttt gtcttttgca
     7681 atccgatgac cttaagcctg ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7741 cggcagcctc ttatagctgc cactcaatga atggcctctt tccccatatc aaccctggcg
     7801 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7861 cactggcggt gagctatctg ctggttggtc tggtcaccaa cttctttcgc ggctgggtga
     7921 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     7981 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8041 cggcccgctt gagctggaag ccacgccggg caaaaaaatc gtctatatca ccgccggtac
     8101 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8161 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8221 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8281 caagtccagt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8341 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8401 tgacaccagc aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8461 tatgggttct gccgttgccg tgttgtttca atctggtcgt gacaccatcg acactgtatt
     8521 aaaaaccatt ctgccgttta tggcgttcgt ctcggcgctc attggcatca ttatggcttc
     8581 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8641 ggtcatgctg gcgctcatct gctccttccc gctgctttca cctttcctcg gcccaggcgc
     8701 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8761 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8821 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8881 tgtactggtg agccactttt taaccggcgc accgactgta ctgatcgcct ggtttgtctc
     8941 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9001 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9061 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9121 cactccatcc cggtttgcaa tttacgctcg ggcagcatcg ctatccggtg accgctgttg
     9181 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9241 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9301 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9361 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9421 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9481 ttaacgccga atatggtgaa ggaacagcgt atagttttgg tgctgacgcc accagcgagc
     9541 aaagcgtcct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9601 tctacagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9661 accgtgaatc tggtgggtta tttcctgtgt gcccgtgaat tttctcgttt gatgatccgc
     9721 gacgggattc agggtcgcat tattcagatc aactcgaaat ccggcaaagt gggcagcaaa
     9781 cacaactctg gctacagcgc agcgaaattt ggtggcgtcg ggctgactca atcactggcg
     9841 ctggatctgg cggagtacgg cattacggtg cattcactga tgctcggtaa cctgctgaaa
     9901 tcgcctatgt tccagtcact gttaccccaa tacgcgacca agctgggtat caaaccggat
     9961 caagtcgagc agtattacat cgacaaagta ccgctcaaac gcggctgcga ttatcaggat
    10021 gtgctgaata tgctgctgct ctatgccagt cctaaggcgt cgtactgcac cggacagtcg
    10081 atcaatgtca ccggcggtca ggtgatgttc taatcaacag cggagatccg ttaaggatct
    10141 ccgtgagact aatagaatgc ctgatgcgct acgctcatca ggcatacagg acttccgcca
    10201 ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat cgcctggtgt
    10261 gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt cgacacacta
    10321 tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc gctttaaacc acgggtcgtg
    10381 gtcgccatcg ccctggacga tcagcagcgc atcgtcgaca ccttgtttat gaaaggactg
    10441 accgtcttcg cacgaccgca aaaaattccc gcaattaccg gtatgcatgc gggtgattta
    10501 cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc attggcgctt
    10561 aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt atcattttga
    10621 aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc cattctggag
    10681 tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt tgacaccaca
    10741 ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg caggaaccgt cattcgtact
    10801 tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca taaaacactc
    10861 atcaacaccc acaagaaaga gctgatagca gaagctgccg ttagttttat tcatgatggc
    10921 gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct gctctcgcgc
    10981 tttaataaca tcacggtgat gaccaacagt ctgcatatcg tcaatgcgct atctgaactg
    11041 gataacgaac aaactatcct gatgccaggc ggaacgtttc gtaaaaaatc ggcctcattt
    11101 cacggacagc tggcagagaa tgccttcgaa catttcagtt tcgataaatt gtttatgggc
    11161 accgacggca tcgatctcaa tgcgggcgta accaccttta acgaggttta taccgtcagt
    11221 aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc aaagtttggc
    11281 cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctgat taccgacgca
    11341 ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt gatcataacc
    11401 ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa tgctggagtt
    11461 gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg ccgccaatat
    11521 catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg gccacattgg
    11581 taagaaaatc gccgcaacac ttgccagtac cggcacatcg gctttttttg tccatccggc
    11641 agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc tgtttatctc
    11701 ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag ataaatctat
    11761 cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg caaaagcggt
    11821 gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga cctccagcac
    11881 cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg cgcgcggatt
    11941 taatgaagaa gattttgccc gctcccaccc agccggggca ctgggcgctc gcttgctgaa
    12001 taaagtgcat catctgatgc gccgtgacga tgccatccca caggtggcgt taaccgccag
    12061 cgtgatggat gcgatgctgg aactcagccg caccggtctg gggctggtgg cggtatgtga
    12121 cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct ggctggtagg
    12181 cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca ccacgttaca
    12241 ggcgcaaagt cgcgccatcg acgccaaaga gatcctgatg aagcgcaaaa tcactgccgc
    12301 accggtggtg gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg atttctatca
    12361 ggccgggatt atttaatcct tcattcccag acgtttcgcc agccgatgca ggttggcgac
    12421 gtcggtttcc agcatccgcg cgcaggcagc ccagttatga tgattttgtg ccagtgcctg
    12481 gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct taacaacggg
    12541 caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat gttgcgcctc
    12601 aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc gatgaatagc
    12661 atgttccagt tcgcgcacgt tccccggaaa acgatagtgt tgcagtaaat ttcgcgctcc
    12721 ggcacttaat accacgcggg agagccccag ccgcaaacga cactgctcgc agaaataccc
    12781 cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa gtggaaacac
    12841 gctcaggcga tgaaacaagt cagcgcgaaa tcgccctgcc atcacctctt cgcgtaagtc
    12901 gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat cgccaacgcg
    12961 ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca acgacaactc
    13021 gccgatctca tccagaaaca gagtgccgtt atccgccatt tcaaacttcc cgctgcggtt
    13081 actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt ccgccacact
    13141 ttccggcagt gcagcacagt tgagatagac cagcggattc accgcccgtg gcgaggcttc
    13201 atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga tcaggacgtt
    13261 gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc caggggacaa
    13321 gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg gcatcatatt
    13381 ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg ccagcgcagc
    13441 aatcagccgt agctcttcgt cgctgaaaac atcgaactga tcgggctgca tcccgtcgag
    13501 cgtcagtgcg ccgatcaggt tttgcccggc aaacaatggc agaccaacgc aggcgtgaac
    13561 cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt cgctgtctgc
    13621 gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat gcccttccag
    13681 cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg gaataaactg
    13741 ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac gtagcgtggt
    13801 gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga tggcgatatt
    13861 cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac tatagatatt
    13921 gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc ataattttat
    13981 ttatagagta aaaacaatca gataaaaaac tggcacgtaa tctgcaatta gcaagacatc
    14041 tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa aaataacatt
    14101 cattgggttg gtcaacgtga ctgggaagtg cgtgattttc acggtacgga atataaaacg
    14161 ctacgcggca gcagttacaa cagctacctc atccgcgaag aaaaaaacgt gctgatcgac
    14221 accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga aatcgatctg
    14281 gcggatatcg attacatcgt gattaaccat gcagaagagg accacgctgg ggcgctgacc
    14341 gaactgatgg cgcaaattcc cgatacgccg atttactgta ctgccaacgc tatcgactcg
    14401 ataaatggtc atcatcatca tccggagtgg aattttaatg tggtgaaaac tggtgacacg
    14461 ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa cgccaatgct gcactggccg
    14521 gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga tgctttcggt
    14581 cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga gcttttcgag
    14641 cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt aacaccgaaa
    14701 attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc tcacggcgtg
    14761 gtatggcgcg ataacccgac gcaaattgtc gagctgtacc tgaaatgggc ggctgattat
    14821 caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg catgatggct
    14881 gacgctatcg cccaggggat tgcggaaacc gacccacgcg tggcggtgaa aattttcaac
    14941 gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa aggcgtgctg
    15001 gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccggtct ggtggaggag
    15061 atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggctctca cggctggagc
    15121 ggcggtgcgg tggatcgtct ttccacgcgc ctgcaggatg cgggtttcga aatgtcgctt
    15181 agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg tgaacacggt
    15241 cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt gaatacggtg
    15301 gttaaagaag aaacctctgc caccacgaag tccgacctcg gcccacggat gcagtgcagc
    15361 gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt tgcgccagga
    15421 acgccgtgga gtgaagttcc ggataacttc ctctgcccgg aatgctctct cggcaaagac
    15481 gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc attggttcgg
    15541 gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccagt attccattaa
    15601 ccctgatcgc cgccgacagc atggatgagt acaacaaacc ggacctcagc catgttatca
    15661 gccaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt gccgggcagt
    15721 ttaatctgca cctgtttccg cacacctggg tgacggatat cgatgccgaa gcccatgtgg
    15781 tgaaaagcca gaataatcag tggcaatacg acaagctggt gctggcaact ggtgccagtg
    15841 cctttgtccc gccagtgcct gggcgtgagt taatgctgac gttaaatagt cagcaagagt
    15901 atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc ggcggtggtt
    15961 tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc acgctaatcg
    16021 acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc cgcttgcagc
    16081 atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag gggctggaaa
    16141 aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa gtggatgcgg
    16201 taattgccgc caccggactg cgcccggaaa cagccctggc acgacgcgcc gggctgacga
    16261 ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgacgat atttatgcgc
    16321 tgggcgattg cgcggaaatc aacggtcagg tactgccatt cctgcagccg attcaactta
    16381 gcgcgatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa ctaccggcga
    16441 tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa acccagcgtc
    16501 aggatttacg ctggcaaatt aataccgaac gccagggaat ggtggctcgc ggtgttgacg
    16561 atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag gcatttggat
    16621 tgttgaaaac attgccgatg taggtgggct actgtgccta aaatgtcgga tgcgacgctg
    16681 gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcgt ttacgccgca
    16741 tccggcaatg gtgtccaaat gcaacacgtt ttatccgttc tggacttcac ccgctaacca
    16801 acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg ccggtaaact
    16861 ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg
    16921 gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg tggcctgctc
    16981 acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg cgcgttgatt
    17041 aaccggtgcc tgccagttca gccactgctg ccagaaagcg gcgagatcca gttgattgtc
    17101 caccagcggc atcgtcaccg gatgcgtcac tccgtggcat gaggctgcga gcgcctccag
    17161 agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca gtgccgccgc
    17221 cactgcatcg aacaaccgcc cacacgatga cgccagcggc gcgttaattc cacgctcaat
    17281 ggcccgcgcc agcacgctcc agttttgctg ttgcacactt gctgtttcag ggtaattctg
    17341 ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct gcttcgctgc
    17401 caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac attcgcgata
    17461 gttcacccgc aggcactcgc cgccccacaa agcgccgttc tcccccatac cgataccgtc
    17521 gagcgtcaaa gcaatgacat caccgccatc caacggccac tgatgctctg ccagacacgc
    17581 cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt cacgcgccca
    17641 ctggctggag acatagcccg gatgcgcgtc atgcacaacg tattgcgggg taaaatcgta
    17701 gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat cgtcacttaa
    17761 atcgcccaga tgctgactca acaccgcttg ttcaccgcgc accaggcaga aggtattttt
    17821 cagatccgcg ccgagacaca gcacaggcgg aacattttta aagcccggag gcaaagccag
    17881 cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc gcaccaccga
    17941 atcatccatc cgctgcacga tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg
    18001 caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca ggttgccgga
    18061 ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg ggttcgcagg
    18121 caacattacc ccgacttcgt taaggtcagg ggcgatatca tcacaaagct caggaacgta
    18181 ttttttatcc accagcacaa tcggcgcggc gggcgtggta agcaactggc gcgcagcgtc
    18241 tggtaaaccg tcagccactg gcaacatgac cgccagcggt ttcgccgggc gatgtttgcg
    18301 cgcccgaagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat gaaatccgcc
    18361 aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagccg cctgtaatgc
    18421 cgcctcttgt tctgcatgtt caccatgact tacccattca agatgtgggc cacactccgg
    18481 gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcacggtact ctttgtcaca
    18541 agccggacac aacggaaacg ccgccatcac ggtaaacggg cggtcgtaag gcatggcgcg
    18601 aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac gataacgccg
    18661 ttcgcctggg gtattcattt cggcaaggca agcagggcaa gtagcggcat ccgggacaat
    18721 ttgcgtattc atggcacctc ccgcgctctg gcggatggtg aactcagtgg gcagttgtga
    18781 ccagatgtac ggctcacgct cgacgctatc aatacgcgcc agcggcgggc agtgctgatg
    18841 caattgaaca agtaacgttt ccgggtcttc ccgcagccgg acttctacgc catcgccgtc
    18901 attacagaca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa acggacgaaa
    18961 accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaagatg tgttttttgc
    19021 cattgagtta ttcccgccat catgaattgc gtaacccgtc ctgccggaca cgactgcgtc
    19081 gtatccggca gtctcaggtc ggcgataccg ccagctccgt attctacgaa tatttccggg
    19141 aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc cgtacggcgg
    19201 cgtttttctg cactcagttg ttcaagttta ttacgatcga cacaaatcag cgcatgagtc
    19261 gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc acatttattg
    19321 gcttcggctt tgtcagcccg tacattcagc cccgcgccgc tgttgcgaat caccggacgt
    19381 accaccactt ccatcgcgcc atacgggcaa gccacaacgc aggttttgca accaatgcaa
    19441 cgttcctgca tcacatgaac aaacccttta tcacggctga tagcaccatt cgggcagacg
    19501 ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat gttcacacct
    19561 ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca gtcctgattt
    19621 tcctgatgag aaaccacgca cgccacttca caggtacggc aaccaataca tttactcgcg
    19681 tcagcaatga tgaaacggtt catcaaattc tccagcaatg acagttaatg cgccgataca
    19741 ttcacaaatc atgccagttt taattcactg ttatttaagg aaattaattt ctgtaatgca
    19801 ggaaaaaaga tgtcatcgac actagtgacg atgacatgtg atgacaatgt ttatcgcgaa
    19861 agagcaatga gtgagtcgcg gcggatcagt tttccgctga aggttttcgg cggtgagaaa
    19921 tccccgccat cgagcataaa aatcagccgt ccaataattt cctgaatcat ctcagtcacc
    19981 ggaattttta cgctggagag cgccggaacg atataggggg caattgcgat atcatcgaat
    20041 ccgataactg acacctgctc tggcaccgct acgccgcgct cgtgcaacgc tttcatcgca
    20101 cctatcgcca tatcgtcgtt actggcaact aacgcgctaa atttagcccc acgttcgagc
    20161 aacgtttcta ccccttcggc cccgctggcg ggcgtccatt taccgttagc gataagtttt
    20221 tcattgagcg caataccatg ctgcgccagc gcgtctttat acccagcaag acgttcaatg
    20281 ctggtggggg aatccatcga gccggtaagg aaagcaatct cctgatgtcc ggcgtttatc
    20341 aactctgcca cggcattaaa gctggtctgt ttatgatcgc accagacgct atggctgctg
    20401 tttttgcgca ggcggcgatt aagcaccatt atcggctgac tgtgcgcgtc aatgatgtca
    20461 tcgatctcat ccacgcttaa aaaacgcggg taaatcataa tggcgtcgca gcgcaaatcc
    20521 agcagatact gaatcgcctg gcgctcttct tcggcgctgt gtttaccatc tgccaatagc
    20581 aactgccgcc ctttctcttc cgccattcgc gcggcatgaa agagtaattc actaaaataa
    20641 atgccgtggt aaagcgtgtt ggtcactacc aaccccagcg tctgagtact cttcgccgac
    20701 agattgcgcg ccagcaagtt tgggcggtaa ccgctctctt ctaccgcctg aaacacgcga
    20761 tctttagtct cctggctgac gtagccatta cctgaaagca cgcgggaaac ggtcgctttt
    20821 gaaaccccgg cgcgcttcgc cacttccagc atcgtcgtca tcattttcat ccctttacac
    20881 gcaatcaacg cagtgtactg caccgtttgc cgattgtcct tgcgcaatcg gcgggaaaaa
    20941 tattcaggtg accggtttca caaatataaa aaatgaacaa ttaactctct tgcttattta
    21001 gtgacaacta ttcatgattt tgtgaaaccg gtttcctaat tccgtttcag catcggcatt
    21061 tttccgttac gtcgactgat aacaactaca tctaccctac tgataacagg ataaaatccg
    21121 atggccaaaa attatgcggc gctggcacgc tcggtgatag cggcactggg cggcgttgat
    21181 aacatctcgg cggtcacgca ctgtatgacg cgtttgcgcg ttgttatcaa agatgaccag
    21241 cttatcgaca gcccgacgtt aaaaaccatc cccggcgtgc tcggcgtggt acgtagtgac
    21301 aaccagtgtc aggtgattat cggcaatacc gtttcacaag cctttcagga agtcgtcagc
    21361 ctgctgccgg gagatatgca gcccgcacag cccgtgggta aacccaaact cacgctgcgt
    21421 cgcattggtg cggggatcct cgatgcgctg atcggcacca tgtcaccgct gatcccggcg
    21481 attatcggcg gatcgatggt caaactgctg gcgatgatcc tcgagatgag cggcgtgctg
    21541 acaaaaggat cgccgacctt aaccattctg aatgtgattg gtgacggtgc tttcttcttc
    21601 ctgccgctga tggtcgcggc atctgccgcc atcaaattta aaaccaatat gtcgctggcg
    21661 attgccatcg cgggtgtgct ggtacatccg agttttattg aactgatggc gaaagcggcc
    21721 cagggtgaac atgtcgaatt tgccctgatt ccggtcaccg cggtgaaata cacctacaca
    21781 gtgatcccgg cgctggtgat gacctggtgc ctgtcatata tcgaacgctg ggtggatcgc
    21841 attacaccgg cggtgaccaa aaacttcctc aagccaatgc tgattgtgtt gattgccgca
    21901 ccgctggcaa tcctgctgat tggcccgatt ggtatctgga tcggtagcgc catttcggcg
    21961 ctggtttaca ccattcatgg ttatctgggc tggctttcag tcgccattat gggcgcgctg
    22021 tggcctctgc tggtaatgac cgggatgcac cgcgtcttta cgccaaccat cattcagacc
    22081 attgccgaaa ccggcaaaga agggatggtc atgccgtcag agatcggcgc taacctgtcg
    22141 ctgggcggtt catcactggc ggtggcgtgg aaaacgaaaa acccggaact gcgccagacg
    22201 gcgctggctg cggcggcatc agccattatg gcggggattt ccgaaccggc gttatacggc
    22261 gtggcgatcc gcctgaaacg tccgcttatc gccagtctta tcagcggttt tatttgcggc
    22321 gcggttgccg gtatggcggg gcttgccagc cactcaatgg cagcgccagg gttatttacc
    22381 agcgtgcaat tcttcgatcc ggcgaatcca atgagcatcg tctgggtgtt cgcggtcatg
    22441 gcgctggcgg tggtgctgtc gtttatcctc acactgttgc tcggctttga ggatattcct
    22501 gttgaggaag cggctgccga ggcgcgaaag catcagagcg tacaaccgac cgtcgccaaa
    22561 gaagtaagtc ttaattgagg atgaaaatgt cagtatttcc agaaggtttt ttatggggcg
    22621 gcgcgcttgc cgccaaccag tctgaaggtg cgttccgtga aggtggcaaa gggctgacca
    22681 ctgtcgatat gatcccacac ggcgagcatc gaatggcggt gaaactgggg ctggaaaaac
    22741 gttttcagtt gcgcgatgac gagttttatc ccagccatga ggcgacggat ttttatcatc
    22801 gttataaaga agatatcgcc ctgatggcag agatgggatt caaggttttc cgtacctcaa
    22861 ttgcctggag ccgtctcttt ccgcagggcg atgaaatcac gcccaatcag cagggcattg
    22921 ctttttatcg ttctgtcttt gaagagtgta aaaagtacgg tatcgaaccg ctggtcacgt
    22981 tgtgccactt cgatgtgccg atgcatctgg tcaccgaata tggctcctgg cgtaaccgca
    23041 agctggtgga gtttttcagc cgctacgccc ggacctgctt tgaagcattt gatggtctgg
    23101 tgaaatactg gcttaccttc aatgaaatca acattatgtt gcatagcccg ttctccggcg
    23161 cgggtctggt gtttgaagaa ggtgaaaatc aggatcaggt gaaatatcag gccgcgcatc
    23221 accagctggt tgccagtgcg ctagccacca aaatcgccca tgaggttaac ccgcaaaatc
    23281 aggtgggctg tatgctggcg ggcggtaact tctaccctta cagttgcaag ccggaagatg
    23341 tctgggcggc gctggagaaa gaccgggaaa acctgttttt tatcgatgtg caggcgcggg
    23401 gcacgtatcc ggcttactct gcccgcgtat tccgcgaaaa aggggtaacc atcgacaaag
    23461 caccgggcga tgatgaaatc ctgaaaaaca ccgtcgattt tgtctctttc agctattacg
    23521 cctcgcgctg cgcctcggcg gagatgaacg ccaacaatag cagtgcggcg aacgtagtga
    23581 aatcgctgcg taacccgtat ctgcaagtga gcgactgggg ctggggtatt gatccactcg
    23641 gtctgcgtat caccatgaac atgatgtacg accgttatca gaagccgctg tttctggtgg
    23701 aaaacggcct cggcgcaaaa gatgaacttg ctgccaatgg cgagattaac gatgactatc
    23761 gcatcagcta tttacgcgaa catatccgcg caatgggcga agcgattgca gatggcattc
    23821 cgctgatggg ctacaccacc tggggctgta ttgatttagt ctccgcctct acgggtgaaa
    23881 tgagcaaacg ctacggtttt gtctacgtag accgtgacga cgcaggcaac ggcacgctga
    23941 cgcgcacacg taagaaatcg ttctggtggt ataaaaaagt gattgccagt aatggggaag
    24001 atttagagta ggtaacagtg ccggatgcgg cgtgaacgcc ttatccggcc tacatgtcgc
    24061 acgaaccagg aaggcccgat aagcgcagcg catcgggcaa tctggggttt ggcatcagtc
    24121 aaaatcaaag aaaccattcc ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg
    24181 atatcgtcag ggatcgttgt ccagacaaaa tctgactgac cagggattta gagccgattt
    24241 cgttcttcaa atccgcatag gtcagattat gttgatcaat cagcgtgcga agtaacgcca
    24301 cgccaacagg cattgccgca acggctttgt cgaactctgc aaactcttgg ttgttgtttt
    24361 catattctgc aatgcggctc gccagaaagt cgataagtgg attggtgtca tcttcttcaa
    24421 tcaaatattc gaccagttcc agtgcttcac gataatcatc ttctgagtca ctacctccca
    24481 gaaaggggac tgcattgacc agttctcttg ttgctttaac agcgcgtgca gcattagcag
    24541 tcattctttg ttctctctgt agtaacgtgt cagtttgtca tactcggcgt gattagttat
    24601 gtgcttcaca aagaaccgct tattaacaaa gtttatgtag gcaatcaccc ttaggttgtt
    24661 cccacctaca tccagaacgt accatttgtt gcgatattta aaattgtcga ggttgggaaa
    24721 cgccgttcgc atctcttctg gcgtagaaaa atccgtttcc ttaataaccc gatacaatgc
    24781 ctgaagagcc agagcgtcgt taggatattt acgcgcagac tcttcaaatg gcgctttaga
    24841 aatgatgtgc atttttaagt tttaacttcc ttatagtaag taaacagcca catctgcgag
    24901 ttctatcctt atgtgttcgg aaactatagc tcatcaaatc caagttcaca atatgtaaac
    24961 acttgagcgc gatataatta cccccaaaat cctcatagcc actctgcaaa aagtcagttc
    25021 tggttcaccc catcaagaac atcccccttt atcaagcaac gctgcgtctg cgccagatct
    25081 tccgtttgcg ggtcgcgccc ggcgatgggt ttgaggacga gtttacggtt gggggtataa
    25141 gtaaaacgcg tgagcgcggg atagcttaac ttgccaaacc agccgtgaac acgctgccat
    25201 ttggcgtctt ttacctgata cagactggtt atccagtaac cggaatcaaa ctgcatatcc
    25261 agcaattgcg tgtgaccatt gccttgcaaa tccagcaaat catctacgcc agttttgctt
    25321 gcggtgtaga atcccctggg ttcaaagaca cagggacggc cttcgcgcgt gaacgtcata
    25381 agaattaaat gtgcataagg agccagacca ttaccggtac ttggtaacca gataaccaga
    25441 tcctgaatgc cgttacgatc aagatcggcg cgatagagtt gccctttgcc cgctatgttt
    25501 gccagcatac ccacaggcac agaccaggat ttaccggcgc gatctttacc gttcaattgc
    25561 cattcactat catcatttgg catcttttca agcgtcacgc cagcaacgcc cacttttcta
    25621 aaatgccagg tttgctcggc atcataaggc atggtcagga gaggtttcag cgcccgcttt
    25681 tgcgcgtatt cgctgtcact cattggcaga tattcggtaa agctggtggc ctgtgcaggc
    25741 agcactaagc tcaataaaaa agcgcacatc cctgttctga tattcatttc gctgaattcc
    25801 ctgatggaga attattcttc ttccaccgct aactgcgcga agccgccatt cccttcccag
    25861 ccttccagtc gttgataaac ggtttctacc gcatctttaa tcggctgagt catcgggtag
    25921 taaaagccga cgatatccgg ctggatgccg aggaaaatca cttcgccaat gtcttctttc
    25981 aactggtcga taagatagtt cagcggcatg ttatgggtag tcatcataaa catctcggcg
    26041 atatcatccg ggtcgatgat gcggatttcg ccggggttta gccccatatc agtagcgtca
    26101 acaatcagta accgcgtagg gcgcagttca cggatggcga cgatgtcgtt ttccggtgca
    26161 ctaccgccat caatcaccac ccagttacct ttcggcgcgg cggcgcactt ttccgccagc
    26221 agcgggccag cgccatcatc gcccatcatg ctattgccaa cacagagtaa aacgtcagtc
    26281 acgcagtctc ctcaccatca aatagatggc gttttcctga tgaatatcat gcagcatact
    26341 taacagcatt gtactccatt ccttctgctg cgaagtttgc gctgcccggg ctttatcgaa
    26401 tgcgttggcc agcatcggca catggttaat gtcgatgacg atctcaccat atttcggcac
    26461 gccttccatt ttgcggcggg cttcactgcc cgcctccaga gtggcaatcc atgccagata
    26521 ttcatcccac gggcaggtga gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc
    26581 aatcgccagg ctgtaataga ccacctgctg cgcctcggcg ggcgtggcat cgttctcatc
    26641 aataaattta cggctcagtt gactgaacac caccttttca ctcatcggat acgcgcctct
    26701 tcaacaacat gattcagatg gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc
    26761 agccagcgtg caacctgttc ttcgccctgc cctaactgtg tcaggaagtc atcggcaatc
    26821 tgacgaccgt aacgataacc cgccaggcgg cgtgcttcgc gatccacttt cacacgcagc
    26881 ggctgcacca tatcgccatg caggatttcc gcctgttgtt catcctgctc acccgggcca
    26941 cgggcgtgaa ttttctgctc cagcaggccg agcgccattg caaagccgta cagcgtggcg
    27001 gcaggcgttg gcgggcaacc aggaatgtag acatctaccg ggacgatttt atccataccg
    27061 ccccacacgc agtagagatc gtggaaaata ccaccgctgt taccgcaggc accgtaggag
    27121 atacaaattt tcgggtccgg cgcggactgc cacgcacgca gcgcagggga tcgcattgca
    27181 cgggtgaccg cgccggtaaa cagtaaaata tccgcatgac gcggtgaagg aacgacttta
    27241 atgccgaagc gttctgcatc aaacagcggc gaaagcgtgg cgaaaatttc gatttcgcag
    27301 ccgttgcagc cgccgcagtc cacgcgataa acgtaggcag aacgtttgat ttttttcagt
    27361 aacgacgcct tcatgctggc gatggattca tccaccgtca tcgggaccgg aatgccgttg
    27421 gcgtcacggg ggcctaataa attgctcatc agatggcctc tttcatatgg cgagtcagtt
    27481 caatacggtc ggacggcacc aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc
    27541 tttcgcggtg gttttccgcg cggctgtcgc cgttgtgctt aagcagcgca atggcgtagt
    27601 cgatctcttt ctggacggcg aaaggacgat tgcagacgcg gcagttgcac agcgcgaagc
    27661 gggactgttg caggaagtct tctttcttcc acactgccag ttcgtactct tgcgacagtt
    27721 tgatcgccgc cgtcgggcaa acttcttcgc agcgtccaca gaagatgcag cgcccgagat
    27781 taaactccca cgccagctca ccggtggcga ggtcagtttc aaccgttaag gcgtttgacg
    27841 ggcaggcatt gacgcaggcc gcacagccga tgcactgctg cgggttctgc tccggcttac
    27901 cacggaagtt tttatcaacc gcaatcggct ccagcggata agacgaggtc gccgtgccgg
    27961 ttttgatgac ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt
    28021 tacgctcaat gctgtaacgc tcgagttctt tgtacggcac cactttgctc tttttcttac
    28081 gcacatcgac cacggtcatg cggtcggtac aggagtagca agggtccaag ctaccgataa
    28141 tcagcggcgc atcggaaacg gtgttaccgc gcagcatgta gcgcagggtc ggccagttcg
    28201 cgtaggtcgc ggcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc
    28261 agtggatatc gtcgccgcgc ggcgcttcgg caaagcccag cgcgaaacgg tgcggaatgt
    28321 aggtaaagcc ttccaccatc agcggaccgc ccggcaggtt atccagaccg tagtcgatca
    28381 tgttcagcgc ggtataaact tcgttgatac gcactttcag acgggaaata acgtcgcagc
    28441 cctgctcgct gtggacttcc attggcagca ggccataacc gacaaacggg tgatcggcgc
    28501 gagtatcgcg ggcgtgaccg ctggcgcgaa ccatcgggcc aacgttactg aaatcgcggg
    28561 ccatttccgg gtccagacga ccaatgccga cggtgcgctg ttccatgttc ggcgtactca
    28621 gcagcacatc caccagctcc tgcacttcac gacgcatctg ttgcgccagc tggcgggtct
    28681 ggatcatgtc atctttcaac agatcgcgac gaatcccgcc gatcaggttc aggccgtagg
    28741 ttttacgcgc accggtgagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga
    28801 actgcataaa gccggagtca aagccggtaa agtggcaggc gagaccgagg ttgagcagat
    28861 gcgagtgcag gcgttccacc tccagcagaa tggcgcggat catctgcgca cgttctggca
    28921 cctgaatacc catcgcgttt tctaccgacg tggtgtaggc ggtgctgtgg gcaaagccgc
    28981 agatcccgca cacacggtca gagaggaagg tcacttcgtt gtaacccata cgggtttccg
    29041 ccagtttttc catgccgcga tgaacgtaga acagacggta gtcggcgtcg ataatgtttt
    29101 cgccatcgac gaacagacgg aagtggcccg gttcgtcaga agtgacgtgc agcggaccaa
    29161 tcggcacgac gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat
    29221 cggtggtcgg tgccggacgc tgacgataat ccatgctgtc tttacgcagc ggataaagtt
    29281 catccggcag caccagacgg cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    29341 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    29401 ccggtttgtt ggcatcaact tcgacgcgaa ccgttaccca gcacttagtc cccttctcca
    29461 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaaa
    29521 gcaccgacaa ccagcccccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    29581 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    29641 ccggaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    29701 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    29761 cccagtccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    29821 cagcgcgata accaggacgc cgacgaccag tttgacgatg gcaatcacca gcgccagcag
    29881 cagcccaccg gcggtgaagg tttccatctg tccccacggg ataaatacgc cgacgaacat
    29941 ctgcagcacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctgcc
    30001 gctgtattca gagagcgggc cttcctgcag ctcctgctcg gcttccgcca ggtcgaacgg
    30061 cagtttgccc atttcgataa aggtggcgaa cgcacaggca caaagcgcca gtaccagcgg
    30121 gatgctctgg ctcagcggcc agtgataaac ggtgtcggtg atgttgctga tgttggtgga
    30181 accggcaacc tgtgcggcaa cccacagacc aagcagcagc atcggttcga ccagcacgcc
    30241 aagcatcgct tcacggctcg cgccgatagc ggtaaacggg ctaccggtat ccagaccaga
    30301 aatggcaaag aagaaacgcg cgatggcaaa gagatacagt aaggtgatta aatcacccag
    30361 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    30421 gcccaccatc acatacggcg tcaggcggaa cacccagccg gaggcatccg ggccaacgct
    30481 ctggcggccc agcagtttga aaatgtcacg atactcctgc aacacacccg gcccacgacg
    30541 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    30601 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    30661 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    30721 ccttcgcact gccagcccgg caccagcgac accggattca gccatttgcg cagtttcagc
    30781 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    30841 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    30901 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    30961 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    31021 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    31081 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    31141 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    31201 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    31261 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    31321 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    31381 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    31441 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    31501 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    31561 agccccagca ggataatgcc gatattttcc agggtgtggt aagccagcag gcgctggatg
    31621 ttgtgttcca tcaacgcata cagaccaccg acgaacgcgg tgatcatgcc gagcaccagc
    31681 agcgcgatcc cccaccacag cggtgcatta ccgcccagca gtgacagggt taacatgccc
    31741 agcaggccaa ttttcatgac taccgtagaa aacaacgcgg cagctggcgc agaggcgtta
    31801 gcatgtgcct gcggcaccca gccgtgcagc ggaataatcc cggccagcag gccaaagcca
    31861 atcacaccga gcagccagat atcggaaccg agcggcagct gttgcatacg catatccagt
    31921 aggcacagat ccagcgtgcc ataacgctgc cacagcagcc agcaagcaat caccagcagc
    31981 agagtgccaa gacgccccag cgcaaaccac agtttgccct ctttgctgtt gctggtgagg
    32041 aacaccgcac acaaggccat gacttcggcc attaccacga acatgccgag gttgctggcg
    32101 atgacggcgc agacggcggc agccatcaac atattgatct gcaagccgtt gcacttcacc
    32161 tgcgcgtggc gatgccagtc aatgttgtag aggctgacaa acagaccgca cagaccgagc
    32221 gtaatcagcc aaatcgcgtt aagcggagag atttgcacat cgtagcttac cagcgacagc
    32281 gcaccgctca cgccaaccgc gccagtcagt acagtgaagc ccgcggctgc cgtatacaga
    32341 ctaccaaccg cgccgccaat tccagctatc cagccactta acgctttttg aaaagaaaag
    32401 agaaatgcca gaacagcggc ggcgacaaac catgccacgc cgctattgat cagggaaatt
    32461 gcgctcattt agcctctcca ctttgagcct gctgaaacaa ggtgagatcg ccaaagtccg
    32521 tgttaaaggt cagctcacgc ttacgtttgc tgacgcgggc gatatcggtg ttatccacca
    32581 gatgcagggc tttagtcggg cacatccgca cgcaggccgg accttgttca tcaaaactgc
    32641 aaaggtcaca tttgacggcg atcgcgcgaa tacccggcac ccagtcaagc agcgtactga
    32701 tacgcgccgg agcaggcggt gccggtggcg ctttcggggt attggcgttt gccggaatat
    32761 ccagcggacg gctgccggaa aattcaattg cgccaaacgg gcaggcgatg ccgcacagtt
    32821 tgcagcttac gcacagactt tcattcaact gcacggcccc atcaatgcgg gtgatggcgt
    32881 taaccgggca aaccaccgca cagggtgcat cttcacagtg gtgacagagc tgcggcgcag
    32941 attctttttc attcagcatc actctcaggc gcggcattga ttgcaggccg tgctggcgat
    33001 gcgtctctga acaggcggcc tcacaagtgt ggcagccgat acagagcgtg gagtcaacaa
    33061 ttacaaaacg attcaccagg cattcctcag gtgattgtca tttttgacga aaacatgccg
    33121 atgaaatgtc attttcgaca ctcatcgaca cgcccatccc caaacaggcg taacgcctgc
    33181 aaaacgggca aagcctcagc tcatgctgcc gggctttgtc cctttaccag ttggcttaaa
    33241 ttcaccggca cattgttttc aatagcggtg tataagctgt cgtaaacgcc agcgattttt
    33301 tcgagatagt gttccagaac ctgttcgcga tcgcggatgt tgaccttgcc atcaatcatg
    33361 ccgtcaatgc tcagttgcgc ctgcgcaatc cgctgtttgt cttcgccatc ttttgtttcg
    33421 acgtaatact cgatggtgtg gctgttaaag ccatccgcca gggtgacgta aatacgaaaa
    33481 tgttcaaaaa ggacgaaaca gagttctttg tccggcgcgc agctcatggc gtgatgcagg
    33541 cgcgctttcg ataacgtgat cccctgaacc agcgaattgc agtagatgtg ccactggtcc
    33601 tgtaggcgac gatgccgctg tgcgatgtaa tcggctttct cgcttatttc ccaaatagtc
    33661 attgtcaggt tacccgttta acagagatgc cagctttaag cattttctat gccaactttt
    33721 aatttattgt tattaaatag atttttaagc taaagatgaa tttcgtcgcc gtgtcgacgt
    33781 gtcatttcga catcatcgac attattcacc gcagggataa tcaacactgg cacaattatt
    33841 gcttgtagct ggcaatagtt aatgggaggc gatatgcacg aaataaccct ctgccaacgg
    33901 gcactggaat tgatcgaaca gcaggccgca aaacacggcg caaaacgcgt aactggggtc
    33961 tggctcaaaa ttggcgcatt ttcttgtgtc gaaaccagct ctcttgcctt ttgttttgat
    34021 ctggtttgcc gcggcagcgt ggcggaaggt tgtaaactgc acctcgaaga acaagaggcc
    34081 gaatgctggt gtgaaacatg ccaacagtat gtgacgctac tgacccagcg cgtccgccgc
    34141 tgtccacagt gtcatggtga catgctacag attgtggcag acgacggttt acagattcgg
    34201 cggatagaaa tagaccagga gtgagcgatg tgtacaacat gcggttgcgg tgaaggcaac
    34261 ctgtatatcg agggtgatga acataaccct cattccgcgt ttcgtagcgc gccatttgcc
    34321 ccggcggcac gcccgaagat gaaaatcacc ggcattaaag cgcctgaatt tacccccagc
    34381 cagactgaag aaggcgacct gcattacggt catggcgaag cgggcactca cgcgccgggc
    34441 atgagccagc gtcggatgct ggaagtcgaa attgacgtgc tggacaaaaa taaccgtctg
    34501 gctgaacgca accgcgcgcg ctttgctgcc cgcaagcaac tggtgctcaa cctggtttcc
    34561 agccctggtt ccggtaaaac caccctgctg acggaaaccc taatgcgcct gaaagacagc
    34621 gttccgtgcg cggttattga aggtgaccag caaaccgtga acgatgccgc acgcattcgc
    34681 gctaccggca caccagcgat tcaggtgaac accggtaaag gctgccatct tgacgcacag
    34741 atgattgccg acgccgcacc gcgtctgcca ctggacgata acggtattct gtttatcgaa
    34801 aacgttggca acctcgtatg cccggccagc ttcgatctcg gtgaaaaaca caaagtggcg
    34861 gtgctttccg ttaccgaagg tgaagacaaa ccgctgaaat atccgcatat gtttgccgcc
    34921 gcctcgctga tgctgctcaa caaagttgac ctgttgccgt atctcaactt tgacgttgag
    34981 aagtgcatcg cctgcgcccg cgaagtcaat ccagaaattg aaatcatcct tatttccgcc
    35041 accagcggcg aagggatgga ccagtggctg aactggctgg agacacagcg atgtgcatag
    35101 gcgttcccgg ccagatccgc accattgacg gcaaccaggc aaaagtcgac gtctgcggca
    35161 ttcagcgcga tgtcgattta acgttagtcg gcagctgcga tgaaaacggt cagccgcgcg
    35221 tgggccagtg ggtactggta cacgttggct ttgccatgag cgtaattaat gaagccgaag
    35281 cacgcgacac tctcgacgcc ttacaaaaca tgtttgacgt tgagccggat gtcggcgcgc
    35341 tgttgtatgg cgaggaaaaa taatgcgttt tgttgatgaa tatcgcgcgc cggaacaggt
    35401 gatgcagtta attgagcatc tgcgcgaacg tgcttcacat ctctcttaca ccgccgaacg
    35461 ccctctgcgg attatggaag tgtgcggtgg tcatacccac gccattttta aattcggcct
    35521 cgaccagtta ctgcctgaaa acgttgagtt tatccacggt ccgggttgcc cggtgtgcgt
    35581 actgccgatg ggtagaatcg acacctgcgt ggagattgcc agccatccgg aagtcatctt
    35641 ctgtaccttt ggcgacgcca tgcgcgtgcc ggggaaacag ggatcgctgt tgcaggcaaa
    35701 agcacgcggt gccgatgtgc gcatcgttta ctcgccgatg gatgcgttga aactggcgca
    35761 ggagaatcca acccgtaaag tcgtattctt cggcttaggt tttgaaacca ccatgccgac
    35821 caccgccatc accctgcaac aggcgaaagc ccgcgatgtg cagaattttt acttcttctg
    35881 ccagcatatt acgcttatcc cgacgctgcg cagtttgctg gaacagccgg ataacggtat
    35941 cgacgcgttc ctcgcgccgg gccacgttag tatggttatc ggcactgatg cctataattt
    36001 tatcgccagc gattttcatc gtccgctggt ggtcgctggt ttcgaacccc ttgatctact
    36061 gcaaggcgtg gtcatgctgg tggagcagaa aatagcggcc cacagcaagg tagagaatca
    36121 gtatcgtcgg gtggtaccgg atgccggtaa cctgctggcg caacaggcaa ttgctgatgt
    36181 gttctgtgtc aacggcgaca gcgaatggcg cggcttaggc gtgattgaat cttctggcgt
    36241 gcacctgacg ccggattatc aacgattcga tgccgaagca catttccgcc cagcaccgca
    36301 gcaggtctgc gatgacccgc gcgcgcgttg tggtgaggta ttaacgggca aatgtaagcc
    36361 gcatcaatgc ccgctgtttg gtaacacctg taatcctcaa accgcatttg gtgcactgat
    36421 ggtctcctcc gaaggagcgt gcgccgcgtg gtatcagtat cgtcagcagg agaatgaagc
    36481 gtgaataata tccaactcgc ccacggcagc ggcggccagg cgatgcagca attaatcaac
    36541 agcctgttta tggaagcctt tgccaacccg tggctggcag agcaggaaga ccaggcacgt
    36601 cttgatctgg cgcagctggt agcagaaggc gaccgtctgg cgttctccac cgacagttac
    36661 gtcattgacc cgctgttctt tcctggcggt aatatcggca agctggcgat ttgcggcaca
    36721 gccaatgacg ttgcggtcag tggcgctatt ccgcgctatc tctcctgtgg ctttatcctc
    36781 gaagaaggat tgccgatgga gacactgaaa gccgtagtga ccagcatggc agaaaccgcg
    36841 cgcgcggcag gcattgccat cgttactggc gatactaaag tggtgcagcg cggcgcggca
    36901 gataaactgt ttatcaacac cgctggcatg ggcgcaattc cggcgaatat tcactggggc
    36961 gcacagacgc taaccgcagg cgatgtatta ctggttagcg gcacgctcgg ggaccacggg
    37021 gcgactatcc ttaacctgcg tgagcagctg gggctggatg gcgaactggt cagcgactgc
    37081 gcggtactga cgccgcttat tcagacgctg cgtgacattc ccggcgtgaa agcgctgcgt
    37141 gatgccaccc gtggtggtgt gaacgcggta gttcatgagt tcgcggcagc ctgcggttgt
    37201 ggtattgaaa tttcagaagt ggcactgccg gttaaacctg ccgtgcgcgg cgtttgcgaa
    37261 ctgctgggac tggacgccct taactttgcc aacgaaggca aactggtgat cgccgttgaa
    37321 cgcaacgcgg cagagcaagt gctggcagcg ttacattccc atccactggg gaaagacgcg
    37381 gcgctaattg gtgaagtggt ggaacgtaaa ggtgttcgtc ttgccggtct gtatggtgtg
    37441 aaacgaaccc tcgatttacc acacgccgaa cctcttccgc gtatatgcta ataaaattct
    37501 aaatctccta tagttagtca atgacctttt gcaccgcttt gcggtgcttt cctggaagaa
    37561 caaaatgtca tatacaccga tgagtgatct cggacagcaa gggttgttcg acatcactcg
    37621 gacactattg cagcagcccg atctggcctc gctgtgtgag gctctttcgc aactggtaaa
    37681 gcgttctgcg ctcgccgaca acgcggctat tgtgttgtgg caagcgcaga ctcaacgtgc
    37741 gtcttattac gcatcgcgtg aaaaagacac cccattaaat atgaagacga aactgttctg
    37801 gcacacggtc cggtacgccg cattttgtcg cgccctgata cgctgcattg cagttacgaa
    37861 gaattttgtg aaacctggcc gcagctggtc gcaggtgggc tataccccaa atttggtcac
    37921 tattgcctga tgccactggc ggcggaaggg catatttttg gtggctgtga atttattcgt
    37981 tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc
    38041 gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga ctatgagttg
    38101 ttatgccggg agcgcgataa cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc
    38161 cgcctggata tggacgaact ggtcagcgaa gtcgccaaag aaatccatta ctatttcgac
    38221 attgacgata tcagtatcgt cttacgcagc caccgtaaaa acaaactcaa catctactcc
    38281 actcactatc ttgataaaca acatcccgcc cacgaacaga gcgaagtcga tgaagccgga
    38341 accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct ccacgagcgg
    38401 gacgatttag ccccctatga acgcatgttg ttcgacacct agggcaacca gattcaaacc
    38461 ttgtgcctgt taccgctgat gtctggcgac accatgctgg gcgtgctgaa actggcgcaa
    38521 tgcgaagaga aagtgtttac cactaccaat ctgaatttgc tgcgccagat tgccgaacgt
    38581 gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct gaaagaacgg
    38641 ctggttgatg aaaacctcgc cctgaccgag cagctcaaca atgttgatag tgaatttggc
    38701 gagattattg gccgcagcga agccatgtac agcgtgctta aacaagttga aatggtggcg
    38761 caaagtgaca gtaccgtgct gatcctcggt gaaactggca cgggtaaaga gctgattgcc
    38821 cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca tggtcaaaat gaactgcgcg
    38881 gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc atgagcgtgg ggcttttacc
    38941 ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg ataaaagctc tctgttcctc
    39001 gacgaagtgg gcgatatgcc actggagtta cagccgaagt tgctgcgtgt attgcaggaa
    39061 caggagtttg aacgtctcgg cagcaacaaa atcattcaga cggacgtacg tttaattgcc
    39121 gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag agttccatag cgatctctat
    39181 taccgcctga acgtattccc gattcacctg ccgccactac gcgagcgtcc ggaagatatt
    39241 ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc gtctggggcg caatatcgac
    39301 agcattcctg ccgagacgct gcgcaccttg agcaacatgg agtggccggg taacgtacgc
    39361 gaactggaaa acgtcattga gcgcgcggta ttgctaacac gcggtaacgt gctgcagctg
    39421 tcattgccag atattgtttt accggaacct gaaacgccgc ctgccgcaac ggttgtcgcc
    39481 caggagggcg aagatgaata tcagttgatt gtgcgcgtgc tgaaagaaac caacggcgtg
    39541 gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac cctgctgtca
    39601 cggatgaagc ggctgggaat tgataaatcg gcattgattt aactgcaaat tgccggacag
    39661 atctgcctgt ccggcatact attcacgagg ttttttcgga cgatattttt ccggcagttc
    39721 tggtaccgga cgcttgtcat cgatgagatg acgcacggtt aagatcggat gacgccacag
    39781 cattctcggc ccggcccaac gcataatctg tttcatctct tcacgctttg caggctggta
    39841 acagtgcacc ggacactgct tacaggctgg tttctcttcg ccgaacacac atttatccag
    39901 ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg acgcctgcgg
    39961 gcatttcgct tgataaagat cgatcatttt tttaatcgtc agtttttcac gagagatacg
    40021 cttgccggac atgctgcctc catactctta aggtgtattt atattacaac ttaatttgaa
    40081 aggggactat ggctctgaag gaggaggttt attcaaccga cgcaattttg cgtacttgct
    40141 tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc cggaaccagc atcaagaact
    40201 cgccgttcgc ttcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt
    40261 acaacgaaaa taaaaaccat cacaccccat ttaatatcag ggaaccggac ataaccccat
    40321 gagtgcaata gaaaatttcg acgcccatac gcccatgatg cagcagtatc tcaggctgaa
    40381 agcccagcat cccgagatcc tgctgtttta ccggatgggt gatttttatg aactgtttta
    40441 tgacgacgca aaacgcgcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc
    40501 ggcgggagag ccgatcccga tggcggggat tccctaccat gcggtggaaa actatctcgc
    40561 caaactggtg aatcagggag agtccgttgc catctgcgaa caaattggcg atccggcgac
    40621 cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt acgccaggca ccatcagcga
    40681 tgaagccctg ttacaggagc gtcaggacaa cctgctggcg gctatctggc aggacagcaa
    40741 aggtttcggc tacgcaacgc tggatatcag ctccgggcgt tttcgcctga gcgaaccggc
    40801 cgaccgcgaa acgatggcgg cagagctgca acgcactaat ccggcggaac tgctgtatgc
    40861 agaagatttc gccgagatgt cgctgattga aggccgtcgc ggcctgcgcc gtcgcccgct
    40921 gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat ctgcaatttg gcacccgcga
    40981 tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt tgtgctgccg gttgtctgtt
    41041 gcagtatgcg aaagataccc aacgcacgac cctgccgcat attcgttcta tcaccatgga
    41101 acgtgagcag gacagcatca ttatggatgc cgcgacacgt cgtaacctgg aaattactca
    41161 gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg ctcgactgca ccgtcacgcc
    41221 gatgggtagt cgtatgctga aacgctggct gcatatgcca gtgcgcgata cccgcgtgtt
    41281 gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc accgccgagt tgcagccggt
    41341 actgcgtcag gtcggcgacc tggaacgtat tctggcacgt ctggctttac gaactgctcg
    41401 cccacgcgat ctggcccgta tgcgccacgc tttccagcaa ctgccggagc tgcgtgcgca
    41461 gttagaaact gtcgatagtg caccggtaca ggcgctacgt gagaagatgg gcgagtttgc
    41521 cgagctgcgc gatctgctgg agcgagcaat catcgacaca ccgccggtgc tggtacgcga
    41581 cggtggtgtt atcgcatcgg gctataacga agagctggat gagtggcgcg cgctggctga
    41641 cggcgcgacc gattatctgg agcgtctgga agtccgcgag cgtgaacgta ccggcctgga
    41701 cacgctgaaa gttggcttta atgcggtgca cggctactac attcaaatca gccgtgggca
    41761 aagccatctg gcacccatca actacatgcg tcgccagacg ctgaaaaacg ccgagcgcta
    41821 catcattcca gagctaaaag agtacgaaga taaagttctc acctcaaaag gcaaagcact
    41881 ggcactggaa aaacagcttt atgaagagtt gttcgacctg ctgttgccgc atctggaagc
    41941 gttgcaacag agcgcgagcg cgctggcgga actcgacgtg ctggtgaacc tggcggaacg
    42001 ggcctatacc ctgaactaca cctgcccgac cttcattgat aaaccgggca ttcgcattac
    42061 cgaaggtcgc catccggtag ttgaacaagt actgaatgag ccatttatcg ctaacccgct
    42121 gaatctgtcg ccgcagcgcc gtatgttgat catcaccggt ccgaacatgg gcggtaaaag
    42181 tacctatatg cgccagaccg cactgattgc gctgatggcc tacatcggca gctatgtacc
    42241 ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt acccgcgtag gcgcggcaga
    42301 tgacctggct tccgggcgct caacctttat ggtggagatg actgaaaccg ccaatatttt
    42361 acataacgcc accgaataca gtctggtgtt aatggatgag atcgggcgtg gaacgtccac
    42421 ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat ctggcgaata agattaaggc
    42481 attgacgctg tttgccaccc actatttcga gctgacccag ctaccggaga aaatggaagg
    42541 cgtcaccaac gtacatctcg atgcgctgga gcacggcgac actatcgcct ttatgcacag
    42601 cgtgcaggac ggcgcggcga gcaaaagcta cggtctggcg gttgctgctc tggcgggcgt
    42661 accgaaagag gttattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc
    42721 gaacgccgcc gcgacgcaag tggatggtac gcagatgtct ttgctgtcag tgccagaaga
    42781 aacttcgcct gcggtcgaag cactggaaaa tctcgacccg gattcactga ctccgcgtca
    42841 ggcgctggag tggatttatc gcttgaagag tctggtgtaa taacaattcc cgatagtctt
    42901 ttgctatcgg gaatattaac gacaactgac gaatcaaata aaaataccct gtataatagg
    42961 aaagcttatt ttacagggta aaaccatgcc atctacacgc tatcaaaaaa tcaatgccca
    43021 tcactatcgc catatatggg tcgttggtga tattcatggt gaatatcagt tattacaatc
    43081 ccgttaacat caactctctt tttaccctga aaccgactta cttatttcca caggcgataa
    43141 tattgatcgt gggccgaaga gtcttaacgt gctgcgcctg ctaaaccaac cctggtttac
    43201 gtcggttaaa ggcaaccacg aagcgatggc gttggatgca ttcgaaactg gcgatggaaa
    43261 tatgtggctt gccagcggtg gtgactggtt tttcgattta aatgattcag agcaacaaga
    43321 ggcaatagat ctgttgctga aattccatca ccttccacat attattgaaa tcattaatga
    43381 caacataaaa tatgccatc
//
LOCUS       DAQSWQ010000025        43122 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.025, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000025 DAQSWQ010000000
VERSION     DAQSWQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.025"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="R8G18_001414"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="R8G18_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HEE9795159.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="R8G18_001415"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="R8G18_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HEE9795160.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="R8G18_001416"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="R8G18_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HEE9795161.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="R8G18_001417"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="R8G18_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HEE9795162.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="R8G18_001418"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="R8G18_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HEE9795163.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="R8G18_001419"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="R8G18_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HEE9795164.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="R8G18_001420"
     CDS             complement(6574..7338)
                     /locus_tag="R8G18_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEE9795165.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="R8G18_001421"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="R8G18_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HEE9795166.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="R8G18_001422"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="R8G18_001422"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEE9795167.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="R8G18_001423"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="R8G18_001423"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEE9795168.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="R8G18_001424"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="R8G18_001424"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEE9795169.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="R8G18_001425"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="R8G18_001425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HEE9795170.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="R8G18_001426"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="R8G18_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HEE9795171.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="R8G18_001427"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="R8G18_001427"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HEE9795172.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="R8G18_001428"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="R8G18_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HEE9795173.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERVVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="R8G18_001429"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="R8G18_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEE9795174.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="R8G18_001430"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="R8G18_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HEE9795175.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="R8G18_001431"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="R8G18_001431"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEE9795176.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="R8G18_001432"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="R8G18_001432"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEE9795177.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="R8G18_001433"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="R8G18_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEE9795178.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="R8G18_001434"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="R8G18_001434"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HEE9795179.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="R8G18_001435"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="R8G18_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HEE9795180.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="R8G18_001436"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="R8G18_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HEE9795181.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="R8G18_001437"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="R8G18_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HEE9795182.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="R8G18_001438"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="R8G18_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="R8G18_001439"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="R8G18_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HEE9795183.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="R8G18_001440"
     CDS             complement(31717..33003)
                     /locus_tag="R8G18_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEE9795184.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="R8G18_001441"
     CDS             complement(33054..33995)
                     /locus_tag="R8G18_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEE9795185.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPEVYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="R8G18_001442"
     CDS             complement(34010..34780)
                     /locus_tag="R8G18_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HEE9795186.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="R8G18_001443"
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="R8G18_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HEE9795187.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="R8G18_001444"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="R8G18_001444"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HEE9795188.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39286
                     /gene="caiB"
                     /locus_tag="R8G18_001445"
                     /pseudo
     CDS             38069..39286
                     /gene="caiB"
                     /locus_tag="R8G18_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            39360..40913
                     /gene="caiC"
                     /locus_tag="R8G18_001446"
     CDS             39360..40913
                     /gene="caiC"
                     /locus_tag="R8G18_001446"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HEE9795189.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            41022..41807
                     /gene="caiD"
                     /locus_tag="R8G18_001447"
     CDS             41022..41807
                     /gene="caiD"
                     /locus_tag="R8G18_001447"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HEE9795190.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARKLAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41813..42403
                     /gene="caiE"
                     /locus_tag="R8G18_001448"
     CDS             41813..42403
                     /gene="caiE"
                     /locus_tag="R8G18_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HEE9795191.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42612..43007)
                     /gene="caiF"
                     /locus_tag="R8G18_001449"
     CDS             complement(42612..43007)
                     /gene="caiF"
                     /locus_tag="R8G18_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HEE9795192.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT        10349 a        11741 c        11336 g         9696 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtctg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccacccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccca gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cacttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaatgca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    39301 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    39361 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    39421 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    39481 ttgagttaaa tcaggagatt aaccgcacga caaacctgtt ttatacgctg gggattcgca
    39541 aaggcgacaa ggttgctcta catctcgaca actgcccgga atttatcttt tgctggttcg
    39601 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgttg cgcgaggaaa
    39661 gcgcgtggat cctgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattctatc
    39721 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    39781 atgtggcact tcccgctgat gatggcgtga gttcgtttac tcaactgaaa aatcaacaac
    39841 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    39901 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    39961 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac ctgacggtaa
    40021 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    40081 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gtacagaagt
    40141 accgcgccac cgttaccgaa tgtattccga tgatgatccg taccttgatg gcgcagccgc
    40201 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    40261 agcaggaaaa agatgcgttt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    40321 tgacggaaac cattgtgggc attatcggcg atcgccctgg cgataaacga cgctggccgt
    40381 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    40441 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    40501 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    40561 ataccggcga taccggatac tgcgacgaag agggcttttt ttatttcgtc gatcgccgct
    40621 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    40681 ccacccatcc aaaaattcag gatatcgtgg ttgtgggtat taaagattcg attcgcgatg
    40741 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    40801 tccgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    40861 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    40921 agcagggaac taccctgcca actaataatc acgctataaa acaatatgca ttttttgcag
    40981 ggatgttgtc gtccctgaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    41041 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg tccaaaagcg aatgctattg
    41101 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    41161 tacgtgtcgc cattattacc ggtgccggag agaagttctt ttccgcgggc tgggatttaa
    41221 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtgga tttgcgggat
    41281 taaccgaaat tttcaatctc gacaaaccgg ttatcgcagc tgtgaacggc tatgcctttg
    41341 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    41401 tcgccctgcc ggaagccaag ctgggcatcg ttcctgacag cggcggcgtg ctgcgtctac
    41461 cgaagatcct gccgcctgcc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    41521 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    41581 ataacgcccg caaactggcg cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    41641 tgaaagagat cttccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    41701 gcagcggcgt gttgaaacac tatccgtcgg ttctgcattc ggaggatgcc attgaagggc
    41761 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    41821 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    41881 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    41941 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    42001 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcacgg
    42061 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtgat
    42121 tatggatggc gcggtcattg gcgaagagag cattgttgcc gccatgagct ttgtcaaagc
    42181 aggctttcac ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    42241 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    42301 cgggcgctgt catgcatcgt tacatgaaac acagccactg aggcaaatgg aggaaaatcg
    42361 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcggcg
    42421 taaacgcctt atccaactct gctggagagc aaccgtaggc cggataagat gcgccagcat
    42481 cgcatccggc gatagctgtc ggatgcggca taaacgcctt atccaacccg gctggagagc
    42541 aaccgtaggc tggataagat gcgccagcat tgcatccagc aaccaggctg cgtggtatca
    42601 acgaaatgac attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg
    42661 ttaagttccg tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaattta
    42721 tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cacttttacc
    42781 agtcgctggc actggcatcc ccgtccttcc agcttattag ggatcatctt aactgcgcag
    42841 cttatttctg cgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca
    42901 atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat
    42961 tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt
    43021 tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa
    43081 attgtggata tatcgatagt atgcaacttc accgtcgata ag
//
LOCUS       DAQSWQ010000026        41011 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.026, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000026 DAQSWQ010000000
VERSION     DAQSWQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.026"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            216..1880
                     /gene="asnB"
                     /locus_tag="R8G18_001450"
     CDS             216..1880
                     /gene="asnB"
                     /locus_tag="R8G18_001450"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HEE9795193.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2260..2336
                     /locus_tag="R8G18_001451"
     tRNA            2260..2336
                     /locus_tag="R8G18_001451"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2294..2296,aa:Met,seq:cat)
     gene            2345..2429
                     /locus_tag="R8G18_001452"
     tRNA            2345..2429
                     /locus_tag="R8G18_001452"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2379..2381,aa:Leu,seq:tag)
     gene            2453..2527
                     /locus_tag="R8G18_001453"
     tRNA            2453..2527
                     /locus_tag="R8G18_001453"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Gln,seq:ttg)
     gene            2562..2636
                     /locus_tag="R8G18_001454"
     tRNA            2562..2636
                     /locus_tag="R8G18_001454"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Gln,seq:ttg)
     gene            2652..2728
                     /locus_tag="R8G18_001455"
     tRNA            2652..2728
                     /locus_tag="R8G18_001455"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Met,seq:cat)
     gene            2777..2851
                     /locus_tag="R8G18_001456"
     tRNA            2777..2851
                     /locus_tag="R8G18_001456"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2809..2811,aa:Gln,seq:ctg)
     gene            2888..2962
                     /locus_tag="R8G18_001457"
     tRNA            2888..2962
                     /locus_tag="R8G18_001457"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Gln,seq:ctg)
     gene            complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="R8G18_001458"
     CDS             complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="R8G18_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HEE9795194.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4437..5861
                     /gene="miaB"
                     /locus_tag="R8G18_001459"
     CDS             4437..5861
                     /gene="miaB"
                     /locus_tag="R8G18_001459"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEE9795195.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6014..7054
                     /locus_tag="R8G18_001460"
     CDS             6014..7054
                     /locus_tag="R8G18_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEE9795196.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7051..7518
                     /gene="ybeY"
                     /locus_tag="R8G18_001461"
     CDS             7051..7518
                     /gene="ybeY"
                     /locus_tag="R8G18_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEE9795197.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7608..8486
                     /gene="corC"
                     /locus_tag="R8G18_001462"
     CDS             7608..8486
                     /gene="corC"
                     /locus_tag="R8G18_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HEE9795198.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8511..10049
                     /gene="lnt"
                     /locus_tag="R8G18_001463"
     CDS             8511..10049
                     /gene="lnt"
                     /locus_tag="R8G18_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEE9795199.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10127..12004)
                     /locus_tag="R8G18_001464"
     CDS             complement(10127..12004)
                     /locus_tag="R8G18_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEE9795200.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12459..13367
                     /gene="gltI"
                     /locus_tag="R8G18_001465"
     CDS             12459..13367
                     /gene="gltI"
                     /locus_tag="R8G18_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HEE9795201.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13537..14277
                     /gene="gltJ"
                     /locus_tag="R8G18_001466"
     CDS             13537..14277
                     /gene="gltJ"
                     /locus_tag="R8G18_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HEE9795202.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14277..14951
                     /gene="gltK"
                     /locus_tag="R8G18_001467"
     CDS             14277..14951
                     /gene="gltK"
                     /locus_tag="R8G18_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HEE9795203.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14951..15676
                     /gene="gltL"
                     /locus_tag="R8G18_001468"
     CDS             14951..15676
                     /gene="gltL"
                     /locus_tag="R8G18_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HEE9795204.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15794..16729
                     /gene="rihA"
                     /locus_tag="R8G18_001469"
     CDS             15794..16729
                     /gene="rihA"
                     /locus_tag="R8G18_001469"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HEE9795205.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16821..18491
                     /gene="hscC"
                     /locus_tag="R8G18_001470"
     CDS             16821..18491
                     /gene="hscC"
                     /locus_tag="R8G18_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HEE9795206.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="R8G18_001471"
     CDS             complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="R8G18_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HEE9795207.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19999..20706)
                     /locus_tag="R8G18_001472"
     CDS             complement(19999..20706)
                     /locus_tag="R8G18_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HEE9795208.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20808..21362
                     /locus_tag="R8G18_001473"
     CDS             20808..21362
                     /locus_tag="R8G18_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEE9795209.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21372..22799)
                     /locus_tag="R8G18_001474"
     CDS             complement(21372..22799)
                     /locus_tag="R8G18_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEE9795210.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22796..23503)
                     /locus_tag="R8G18_001475"
     CDS             complement(22796..23503)
                     /locus_tag="R8G18_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HEE9795211.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23667..24644
                     /locus_tag="R8G18_001476"
     CDS             23667..24644
                     /locus_tag="R8G18_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEE9795212.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24714..25196)
                     /locus_tag="R8G18_001477"
     CDS             complement(24714..25196)
                     /locus_tag="R8G18_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HEE9795213.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25431..28013
                     /gene="leuS"
                     /locus_tag="R8G18_001478"
     CDS             25431..28013
                     /gene="leuS"
                     /locus_tag="R8G18_001478"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEE9795214.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28028..28609
                     /gene="lptE"
                     /locus_tag="R8G18_001479"
     CDS             28028..28609
                     /gene="lptE"
                     /locus_tag="R8G18_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEE9795215.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28609..29640
                     /gene="holA"
                     /locus_tag="R8G18_001480"
     CDS             28609..29640
                     /gene="holA"
                     /locus_tag="R8G18_001480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEE9795216.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29642..30283
                     /gene="nadD"
                     /locus_tag="R8G18_001481"
     CDS             29642..30283
                     /gene="nadD"
                     /locus_tag="R8G18_001481"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEE9795217.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30307..30918
                     /locus_tag="R8G18_001482"
                     /pseudo
     CDS             30307..30918
                     /locus_tag="R8G18_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31178..31495
                     /gene="rsfS"
                     /locus_tag="R8G18_001483"
                     /gene_synonym="rsfA"
     CDS             31178..31495
                     /gene="rsfS"
                     /locus_tag="R8G18_001483"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEE9795218.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31499..31966
                     /gene="rlmH"
                     /locus_tag="R8G18_001484"
     CDS             31499..31966
                     /gene="rlmH"
                     /locus_tag="R8G18_001484"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEE9795219.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31997..33898
                     /gene="mrdA"
                     /locus_tag="R8G18_001485"
     CDS             31997..33898
                     /gene="mrdA"
                     /locus_tag="R8G18_001485"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HEE9795220.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33901..35013
                     /gene="mrdB"
                     /locus_tag="R8G18_001486"
                     /gene_synonym="rodA"
     CDS             33901..35013
                     /gene="mrdB"
                     /locus_tag="R8G18_001486"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HEE9795221.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35024..36112
                     /gene="rlpA"
                     /locus_tag="R8G18_001487"
     CDS             35024..36112
                     /gene="rlpA"
                     /locus_tag="R8G18_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HEE9795222.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36251..37462
                     /gene="dacA"
                     /locus_tag="R8G18_001488"
     CDS             36251..37462
                     /gene="dacA"
                     /locus_tag="R8G18_001488"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HEE9795223.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37572..37835
                     /locus_tag="R8G18_001489"
     CDS             37572..37835
                     /locus_tag="R8G18_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEE9795224.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37936..38577
                     /gene="lipB"
                     /locus_tag="R8G18_001490"
     CDS             37936..38577
                     /gene="lipB"
                     /locus_tag="R8G18_001490"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEE9795225.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38837..39790
                     /locus_tag="R8G18_001491"
     CDS             38837..39790
                     /locus_tag="R8G18_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEE9795226.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39999..40964
                     /gene="lipA"
                     /locus_tag="R8G18_001492"
     CDS             39999..40964
                     /gene="lipA"
                     /locus_tag="R8G18_001492"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEE9795227.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10295 a        10449 c        10294 g         9973 t
ORIGIN      
        1 ccccgtaaaa tataaccatt caataaaacc cgctaaaaat tatcgattca tcaataaata
       61 catcctcaca ccagcccctt tttcaccaat cgacaatcac cattacgttt tttatttttt
      121 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
      181 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
      241 atatcaaaac agacgcagtt gagcagcgta agaaagcact cgagctgtca cgcctgatgc
      301 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
      361 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
      421 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
      481 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
      541 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
      601 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
      661 ggtatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
      721 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
      781 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
      841 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
      901 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
      961 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccccagttac
     1021 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     1081 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     1141 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     1201 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     1261 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     1321 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg
     1381 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat
     1441 tccttgatgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg
     1501 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     1561 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     1621 cggcgcagca ggtttctgat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca
     1681 cgccaacctc aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc
     1741 cgagcgccgc tgagtgcgtg ccgggcggcc cgtccgtcgc gtgttcttcc gctaaagcga
     1801 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
     1861 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     1921 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     1981 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccaccat
     2041 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg gggaaaaaac
     2101 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
     2161 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
     2221 gcgccccgca acgccgataa ggtatcgcga aaaaaagatg gctacgtagc tcagttggtt
     2281 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     2341 ttttgcggga gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg
     2401 tgcgagttca agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat
     2461 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
     2521 ccagccatct tcttcgagta agcggttcac cgaccggtta ttggggtatc gccaagcggt
     2581 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatcga
     2641 agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
     2701 acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat
     2761 tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct gattccggca
     2821 ttccgaggtt cgaatcctcg taccccagcc aatttattca agacgcttac atcgtaagtg
     2881 caccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
     2941 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagttt tttgcttttc
     3001 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
     3061 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
     3121 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta
     3181 acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
     3241 gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
     3301 ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga acatcaatca
     3361 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
     3421 gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg
     3481 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
     3541 tcgcgatttc cgcctggaat tgcgccatat tcatattctg caactggcga atacgcgccg
     3601 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
     3661 cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
     3721 tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca
     3781 cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct
     3841 tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac
     3901 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt
     3961 tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg
     4021 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
     4081 catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc
     4141 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
     4201 ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga
     4261 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
     4321 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
     4381 aaagcattac actatacgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
     4441 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
     4501 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
     4561 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
     4621 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
     4681 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
     4741 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
     4801 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
     4861 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
     4921 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
     4981 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
     5041 acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc
     5101 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
     5161 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
     5221 tgcatctgcc ggtacaaagc ggttccgatc gcattctgaa cctgatgggc cgtactcaca
     5281 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga
     5341 tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
     5401 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
     5461 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcaacgtc
     5521 tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg
     5581 gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg
     5641 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
     5701 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
     5761 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
     5821 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
     5881 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
     5941 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
     6001 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
     6061 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
     6121 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
     6181 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
     6241 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gagtactgga
     6301 gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
     6361 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
     6421 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
     6481 agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga
     6541 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
     6601 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat
     6661 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga
     6721 cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
     6781 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
     6841 gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga
     6901 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat
     6961 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc
     7021 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
     7081 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg
     7141 gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa
     7201 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
     7261 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
     7321 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
     7381 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
     7441 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
     7501 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
     7561 ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
     7621 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
     7681 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
     7741 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
     7801 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
     7861 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
     7921 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
     7981 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
     8041 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
     8101 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
     8161 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc
     8221 gtcagctgag tcgtcatacc tggaccgtgc gtgcactggc ttccattgaa gacttcaacg
     8281 aagcgttcgg cacccacttt agcgatgaag aggttgacac tatcggtggt ctggtgatgc
     8341 aggcatttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
     8401 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
     8461 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
     8521 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
     8581 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc
     8641 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
     8701 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
     8761 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
     8821 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
     8881 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
     8941 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
     9001 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
     9061 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
     9121 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
     9181 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta
     9241 atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta
     9301 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
     9361 tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc
     9421 tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac
     9481 catacagcta cgaatcagcc gatcgctata acaaaaacca tctagtgccg tttggcgagt
     9541 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
     9601 cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg
     9661 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
     9721 ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc
     9781 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
     9841 ccaacaacgg cattacggcg gtgattggcc cgcaggggga ggttcaggcg atgatcccgc
     9901 agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtatg
     9961 cacgtaccgg caactggcca ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgt
    10021 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    10081 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    10141 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg
    10201 tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg
    10261 aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa
    10321 tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt
    10381 ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg
    10441 cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa ataacctcgc
    10501 cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt
    10561 cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa
    10621 ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg
    10681 tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat
    10741 caatcgctga cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg
    10801 agaggataag gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg
    10861 ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat
    10921 ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg
    10981 acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga
    11041 aaacaacaat gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga
    11101 tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac
    11161 ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt
    11221 tttgccgtcg ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga
    11281 cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa
    11341 gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg
    11401 cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta
    11461 ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata
    11521 ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt gctatcgctg
    11581 caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc tgctcacttt
    11641 cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa
    11701 tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc aggaatgcaa
    11761 acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc
    11821 agggttcacc cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt
    11881 gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg
    11941 caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag
    12001 acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat
    12061 catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc
    12121 ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta
    12181 tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag
    12241 cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg
    12301 caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
    12361 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    12421 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
    12481 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    12541 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    12601 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    12661 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
    12721 taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
    12781 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    12841 atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
    12901 ttgccgacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    12961 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcaca
    13021 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
    13081 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
    13141 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
    13201 tgatggatga caccaccgcg caggtgcaga cctccggtga agcggaaaaa tggtttgata
    13261 aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    13321 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta caagaaccag
    13381 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    13441 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    13501 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    13561 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
    13621 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    13681 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    13741 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    13801 ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    13861 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    13921 caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg
    13981 ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    14041 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
    14101 attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc
    14161 tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
    14221 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
    14281 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
    14341 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
    14401 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
    14461 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt
    14521 ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg
    14581 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    14641 gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    14701 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg
    14761 gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc
    14821 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg
    14881 gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    14941 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    15001 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    15061 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    15121 aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc
    15181 ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga ttatcgaaaa
    15241 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    15301 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    15361 ttccggcggt cagcagcagc gtgtggcaat cgctcgcgca ttgtgtatgg atcctattgc
    15421 gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    15481 cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    15541 ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga
    15601 ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc
    15661 gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta
    15721 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    15781 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc
    15841 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    15901 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    15961 taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    16021 tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac
    16081 gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc
    16141 ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    16201 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    16261 tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    16321 tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc acaaagcaca
    16381 aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    16441 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    16501 tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    16561 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta tgacggttgt
    16621 tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg
    16681 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    16741 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctgtt tatctctttt
    16801 tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc gatctcggca
    16861 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    16921 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    16981 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    17041 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    17101 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    17161 ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc aagcataccc
    17221 gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    17281 ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt tttgatctcg
    17341 gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt gaagttcacg
    17401 cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg
    17461 ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg gcagccttgt
    17521 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt cgctggcagt
    17581 accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat ttgtggttgc
    17641 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca cgtctgaagc
    17701 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    17761 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg agcaccattg
    17821 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagagg
    17881 tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc cagggcgttt
    17941 ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg cgtgtagaaa
    18001 cttactcgac catgcacccg gaacaggatt ctattacggt taacgtctat cagggagaaa
    18061 accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg aagaaaaccg
    18121 gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg cttgaagttg
    18181 acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc ccggtaacat
    18241 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    18301 gcgatatgct aatcaaccgc acctttaagg ccaaactgga agagttgtgg gcgcgggcgc
    18361 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgttgcagt
    18421 caaacgatat ggcccgcgtc gatgaagttc gtcgacgggc gagcgattat ttggctattg
    18481 agatccctta aacacggctg ccggatgcgg cataaacgct gcatccggca tcggcgcaaa
    18541 aaacacatcc tcagctaaac aacatcgcga tttttccgag cgcataacct atcatcagca
    18601 ccgttaagct gattttgata atactggtaa tgctctgtgg tttttcatcc tcatcatttt
    18661 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttca
    18721 ctttcgtcaa cagcatctgg gtgataatgg cctctaatgg atgagcatct ccaccttcca
    18781 gccttgtatt aagacgggcc agcatctctt gtttcgggaa cgcttccagc acaacctgtt
    18841 ccgctacccg atacaaatcc agtaacggtg aatcttgctc tgtctcgcta ccgactctcg
    18901 atttccacca cgcccatgct ttgcgcttga agaataacga ctcaataaac gattgaccta
    18961 acggcgataa ttttccgcta tgtaaaatct ccgcccataa cagccgcgtc cgggcgctct
    19021 caccaggaac atatttcaac ggtttacctt gcgcatctac catgcttcgc gcttcgataa
    19081 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    19141 tatcagcttg tgtagatggg taactttccc agtacgcgca gagatcggca agcaggcttt
    19201 ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg
    19261 gttcttccgc ttgccactgt ttggctacag gaatgagttc cgccatacct gtttgcaccg
    19321 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc
    19381 cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg
    19441 cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga
    19501 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcca
    19561 ttccttcgct gtcattaaac gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat
    19621 ttaatgaaag atatcgcgct tctatagcga ttgcacacag cggccagcgt aattgatcga
    19681 catcctccat gttccaggta tttaattgct gaataaattt ctgccaggcg gagggctgaa
    19741 aacgatcact ttctgaatcc agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg
    19801 tggcatcttt ttcttcatca tctgcttcct cgacaggatt taccgccagt cgcagcgcct
    19861 cttcataggc ctggcgtaat gctttaaacc cctgcggatc ggtttcggga tgacacaacg
    19921 gtaagcgagc aagataagcc tggcggataa tgtctatttg cgtcgtgctt tcaatttcga
    19981 ggatttgcca acatgttttc ataggtcgct cacatcctta agcgttaccg ggcattcagg
    20041 ctggacagtt aatggttgcc agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag
    20101 ctcttcgaac atccgactcc cggcgctatc gtatttgtac agggttagcg cctcgcgtag
    20161 cgaagcgttg tcttcgcact gagaagattg ccagtagaga cgccctagtg aataggcaga
    20221 aaaatagtgc atccagctgt gatagtaatg gtgagctcgt gcatagacgc gcgattgcag
    20281 ccataaactc tcttcttccg tcagccagtt gttgagcaca ccgacccgtg acagaaaccc
    20341 cattcgaaca taatcccagg ctttgattcc cccttctcca cagcacatag cggtttgggc
    20401 aacgaattgg gtataaatca accctctctc cgagccacca gcaattaatg ctttccactc
    20461 ttcaggggaa gagcgttgcc atgtcagata taagccagct aaatcattgg catgaccatc
    20521 atcagtcatc tgataaatag tctgaattaa atcatcacga ttatttattc cccaggatga
    20581 tgagagatcg aattctttta tcccagtata catttttggc gagtcatgcg cgggtttgca
    20641 actctgatta agtatttcca tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt
    20701 attcattttt taataatccc ttaagtgcat taactgacta cttttttatt attgatgagt
    20761 ataaacttga cgctttcaaa ataaaaagaa aatcgaagca ttcacacatg aataaaaaat
    20821 caatgtatat attcgcaatt tttatagttg cagcaattac ctgtattagc caacccaaga
    20881 aaacgacgtt gcgtgataaa gccatggtga attatgcctt tgattattta agatcaccag
    20941 gcagtctacc gttaaccacg gcagccacgg agctttccgc gattcatggt cactcaacat
    21001 cgcaatatcg ccttggcgaa ttttatcttc atggtagcga cggtaaacca ctggattata
    21061 cacaggcgag atactggtat gagcaatcag cagaacagga aaatccgcgc gcacaaagca
    21121 aactggggtg gatctacctg aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc
    21181 tctggtataa ggaagcagct gaacaagggt atgctcatgc tcaatatact ttgggtttga
    21241 tctacagaaa tggcactggt attaatgtta accattatga atctcaaaaa tggttaaaac
    21301 tggccgccaa acaacattac aaaaatgcgg aaagattact tgccggactt cccgcacatt
    21361 aacgtcgact gtcacccgaa gagatggagt atttttccta tcaaaccggc aaagataaaa
    21421 atgtagaaaa taattttgat gacgcccaga cagcccagcc cgccatcgtc aacgggcatt
    21481 ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt
    21541 aataatcgac gaataattaa tgcttccagt ggacaacgat cttctgcatc aaggcggctc
    21601 acaatcggca ttaatgattc ttctgtcggt tggctggcct gcactaattg tttggcaact
    21661 aaatataatg gccataaggg atgttctgta tcacgcgtga gtgagatccg cggcttacgt
    21721 cctttgtgca aaaccatctc gaccagtgaa cgccccagcg cagagagttt ttcatcgctg
    21781 agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc cggggacata aagtagctgt
    21841 ttaccctgat cgttaaccag ttcacgagct tcaatcatca tcaccactaa cgggtaataa
    21901 tcgacccgat gctgacggca ccattgcaat attaaagcat ccgcctgggt ggagggatac
    21961 tcgcgccagt agtcacaaag ttctgccagc aagctttcgc cttctccaca atagacgcgt
    22021 tgcgcgtatt cataataacg cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg
    22081 gcgtaatcca gaagttcggg gatatccagg cgacctgcgc tataccagcg gagtaaattt
    22141 ctgtgcagtt ttgcatcatc ggggatgatc actgcgcgat gttgcgctaa atacaatgtc
    22201 agccattctg gatgcgatga ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca
    22261 ttttgcacgg caacgggcag atgcagaatg gtctggaagt taaaaacatt ccccttcgca
    22321 atggcatata aaaaggattc cagttcttct tcgtctattt ccccgacgtt ttcctcctgc
    22381 aaccaccgca atctttctgc taataacacc acgcactcga aggataaatg ggcagtgttc
    22441 atggcgattg tgcacagcga ccagcgtaat tcatcaatct cttccatcga gcaataattt
    22501 aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac gttcgctatc agaggcaagt
    22561 aacgcgcgaa aggcgagcag aatttcatgt tctgctacct catattcttc tggttgccaa
    22621 acagatttag caggcgactg cgcaatccgg agcgcctcct catacgcctg acgaagttgt
    22681 ttaaaaccct gcggatcggt ttctggatga aaggacggta acagcgccag ataagcgcgg
    22741 cggataatat cgacgtcagt cgtttcctct atttcgagga tcttccagca atttttcata
    22801 ggtcgctcat atccttaagc gtttccgggc atgtggggta gtcagccagt ggttgccagg
    22861 acaaggcagg gtaatacgca tcatcactgg caaataattg ataaaacata tcattgccgg
    22921 aatcgtcgta ttctttacga gctaagaatt cgcgtaataa aggcagatga tcatcttcgg
    22981 gagactgcca atattgccga ccaaaggtat aaccggcaaa gtattgccgc cagttactat
    23041 aataacgtag agcccgtaga tgaatacgcg actggatcca tagactttct tcttcactca
    23101 accagttatt aagtacgccc attcgactta aaaaacccat ccggacataa tcccatgctt
    23161 taattcctcc ctcaccacag cattcggctg tgctggcgac aaattgcgca taagcctgac
    23221 cttgctcatt tagttcagca agatagtcac gccattcaca tggcgaataa cgaaaccagc
    23281 ggtgataaaa ccctgccaac cgagcggcat gaccgttatc gatcattcgg tgaattgtgc
    23341 gcagcaggtc atcacggtca tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac
    23401 ctaaatacat tttgggggat gaatgcgaag gtaaacagca ttcattgcgt atttccatcg
    23461 gagtggaaag tgcaaacaat attttttgcg accccatgtc catataattt atccttaaac
    23521 attcatttaa tgaatgtaaa ctacatttcg agactcagtt atttatactt ttctgacgca
    23581 acataatacg cataaaataa ggcttacgca aaaatggaac acataaaaat ataatatgtt
    23641 ttatctacaa ccataaggaa atgataatgg tttttacgtc aagttgctgc gataatttat
    23701 caatagatga gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg
    23761 ggttttatta taatcgcgat agcgcaattg actctccgga cgacgaaaag gccttttact
    23821 ggctgaagct ggccgctgag caaggtcatt gtgaagcaca gtattcctta gggcaaaagt
    23881 ataccgagga taaaagccgt cataaagata atgagcaagc catcttctgg ctgaaaaaag
    23941 ctgccctaca aggccatact ttcgcttcca acgcccttgg ctggacactg gatcgtggag
    24001 aagcccccaa ttataaagaa gcggttgtct ggtatcagat agccgcggag agcggaatgt
    24061 cttatgcgca aaataatctt gggtggatgt acagaaatgg caacggagtc gcaaaagact
    24121 atgcgctggc atttttctgg tacaaacaag ctgcattaca aggccatagt gacgcgcaaa
    24181 acaatctggc cgatctttat gaagacggaa aaggcgttgc tcaaaacaag acactcgccg
    24241 cattctggta tttgaaaagc gcacagcagg gtaatcggca cgcccagttt caaattgcat
    24301 gggattataa cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc
    24361 tgaaggctgc cgctcaggaa agcgtcggcg cttacgtcaa catcggttat atgtataaac
    24421 acggacaagg cgttgagaaa gattatcagg ctgcctttga atggtttacg aaagccgctg
    24481 aatgcaatga cgccactgcc tggtataacc tggccattat gtatcactac ggagaaggaa
    24541 gacctgtcga tctccgacag gctctcgacc tgtatcgtaa agttcagtca tccggaacca
    24601 gagatgtcag tcaggaaatt cgtgagactg aagatttact gtagagataa aaacaagggc
    24661 cggggaattt cattcggccc atattctctt tattgctgtg cgaaatcaga ctattacggc
    24721 tcaaacgggc gtctctggaa ctggtcatga ccacatttcg ggcatagcgt cagcacttcc
    24781 ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga ccagatttcc cagcccgacc
    24841 acttctccgc tgtgataaac cccatgatga ttgaggtcct ggaaaacttc gcgccattca
    24901 agctgcgttt tatcggtgat atctgccagc tcctgccata agctttcttt aatcacccgc
    24961 ataaagacgc tgtcagattc ttctttcagg ctctcttcat agctcatggc gaactcttcc
    25021 aggtcacgtc ttacagctcg cgtcagctca tcgacctcgg ttcgcgttaa ctcccctgtt
    25081 tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc gccattgcgc
    25141 aggcgttcgc tcagtgacgc gaccagttca cggtaatatt gagcaacctt gttcatcgtt
    25201 tcatctcccg ggtcgttaac tgactataat aatagacgtt ctttgttcct tgctttgcta
    25261 atacgcccac aaactctgta aattaatgta tgaataatcc gggcatcatt tcaacgtcga
    25321 gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc
    25381 acctcaacgc tacatttgta gccgtattga aaacaggacc actggctgcc atgcaagagc
    25441 aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat
    25501 ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc
    25561 cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc
    25621 gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc
    25681 tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca
    25741 acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac tggagccgcg
    25801 agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc accgagctgt
    25861 ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga
    25921 ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg
    25981 aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca
    26041 acgatctgga taaactggat cactggccag acaccgttaa aaccatgcag cgtaactgga
    26101 tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa cgactatgac aacatgctga
    26161 ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg gtagctgcgg
    26221 gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc tttattgacg
    26281 aatgccgtaa caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg
    26341 atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa
    26401 acttcgtatt gatggagtac ggcacgggcg cagttatggc ggttccgggt cacgaccagc
    26461 gcgactacga gtttgcctct aaatacggcc tgaacatcaa accagttatc ctggctgctg
    26521 acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg ctgttcaact
    26581 ctggcgaatt caacggtctt gaccatgaag cggccttcaa cgccatcgcc gataaactga
    26641 ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc
    26701 gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc
    26761 cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg gacggcatta
    26821 ccagcccgat taaagcagat ccggagtggg caaaaactac cgttaacggt atgccagcgc
    26881 tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat gcgcgctaca
    26941 cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac tggctgccgg
    27001 tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac ttccgcttct
    27061 tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg aaacagttgc
    27121 tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta
    27181 actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag
    27241 cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga
    27301 agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac accgttcgtc
    27361 tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa tccggtgtgg
    27421 aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt ttacgagcac acagcaaaag
    27481 gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg
    27541 atgtgcataa aacgatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca
    27601 ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcacca accgatggcg
    27661 agcaggatcg cgctctgatg caggaagcgc tgctggccgt tgtccgtatg cttaacccgt
    27721 tcaccccgca catctgcttc acgctgtggc aggaactgaa aggcgaaggc gatatcgaca
    27781 acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg ctggtcgtgg
    27841 tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttct ggtggacgca acggaagaac
    27901 aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat ggcgttaccg
    27961 tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg
    28021 aggaagcgtg cgatatctgg caacattgtt gttatctctg gcggtgttaa tcaccgccgg
    28081 gtgtggctgg catctgcgtg ataccacgca ggttccttcc actatgaagg tcatgatcct
    28141 ggactcaggc gatccgaacg ggccattaag ccgtgcagtg cgtaaccagt tacgtctgaa
    28201 tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac gttccatccc tgcgtttggg
    28261 taaagtgagc atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa cagcagagta
    28321 tcagatgatc atgacggtga atgcgaccgt gttgatcccc ggccgtgata tctacccgat
    28381 tagcgccaaa gtcttccgtt cgttctttga taacccgcaa atggcgttag cgaaagataa
    28441 cgaacaagac atgatcgtaa aagagatgta cgaccgtgct gccgaacagc tgattcgtaa
    28501 gctgccaagc atccgtgctg cggatattcg ttccgacgaa gaacagacgt cgaccacaac
    28561 ggatactccg gcaacgcctg cacgcgtctc caccacgctg ggtaactgat gattcggttg
    28621 tacccggaac aactccgcgc gcagctcaat gaagggctgc gcgcggcgta tcttttactt
    28681 ggtaacgatc ctctgttatt gcaggaaagc caggacgctg ttcgtcaggt agctgcggca
    28741 caaggattcg aagaacacca cactttttcc attgatccca acactgactg gaatgcgatc
    28801 ttttcgttat gccaggctat gagtctgttt gccagtcgac aaacgctatt gctgttgtta
    28861 ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc tcacactcac cggacttctg
    28921 catgacgacc tgctgttgat cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc
    28981 gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac accggagcag
    29041 gctcagcttc cccgctgggt tgctgcgcgc gcaaaacagc tcaacttaga actggatgac
    29101 gcggcaaatc aggtgctctg ctactgttat gaaggtaacc tgctggcgct ggctcaggca
    29161 ctggagcgtt tatcgctgct ctggccagac ggcaaattga cattaccgcg cgttgaacag
    29221 gcggtgaatg atgccgcgca tttcacccct tttcattggg ttgatgcttt gttgatggga
    29281 aaaagtaagc gcgcattgca tattcttcag caactgcgtc tggaaggcag cgaaccggtt
    29341 attttgttgc gcacattaca acgtgaactg ttgttactgg ttaacctgaa acgccagtct
    29401 gcccatacgc cactgcgtgc gttgtttgat aagcatcggg tatggcagaa ccgccggggc
    29461 atgatgggcg aggcgttaaa tcgcttaagt cagacgcagt tacgtcaggc cgtgcaactc
    29521 ctgacacgaa cggaactcac cctcaaacaa gattacggtc agtcagtgtg ggcagagctg
    29581 aaagggttat ctcttctgtt gtgccataaa cccctggcgg acgtatttat cgacggttga
    29641 tatgaaatct ttacaggctc tgtttggcgg cacctttgat ccggtgcact atggtcatct
    29701 aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa tcatccctaa
    29761 taatgttcct ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta aacacatgct
    29821 tgaactggcg attgccgaca agccattatt tactcttgat gaacgcgagc taaagcgcaa
    29881 tgccccctct tacactgcgc aaacactgaa agagtggcgg caggaacaag gaccggacgt
    29941 gccgctggcg tttattattg gtcaggattc actgctgacc tttccgacct ggtacgaata
    30001 cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt acccacttga
    30061 aatggcgcaa ccgcaatacc agcaatggct ggaagatcat ttgacacata acccggaaga
    30121 tcttcacctt cagcctgccg gtaaaattta tctggctgaa acgccgtggt ttaacatctc
    30181 ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga
    30241 accggtactg acttacatta accaacaagg cttgtatcgc tgatacctgt cgttacggag
    30301 ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc aagcgaatat cgatggtctt
    30361 tacagcggtc atgcgcccac ccccctgacc gcgcgcggta ttgagcaagc gcaaaatctg
    30421 catacgctgc tacatggtgt ttcctttgat ctggttttat gcagtgaact ggaacgggca
    30481 cagcataccg cacgactggt tctcagtgac cgccagctcc ccgtgcaaat catccctgaa
    30541 ctcaacgaaa tgttttttgg cgactgggag atgcgacatc atcgcgacct catgcaagaa
    30601 gatgccgaaa actatagcgc gtggtgcaat gactggcagc atgcaatccc cacgaacggt
    30661 gaaggatttc aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct tagtgaattt
    30721 cagcactatc agaatatttt agtcgtcagc catcagggtg tactgagtct gttaatcgcc
    30781 cgtttaattg gcatgcctgc cgaggctatg tggcattttc gcgttgacta ggggtgttgg
    30841 agcgccattg atatcaacca aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc
    30901 ggggtcgaga atgcatgact tttctgtttt tttacgggta agccgcaacg accattgaca
    30961 gacccgggca ggctgatatt ctccgcagcc agactttttt cgccagacac gactttgtag
    31021 aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt
    31081 tcgaaagccg attcggcgac aattgtcccg aaatcgcctc tggttcaggt atactgacag
    31141 accattttta tctatttgat tcacccaggg ggaaaacttg cagggtaaag cactccagga
    31201 ttttgttatc gacaaaattg atgacctcaa aggtcaggac atcatcgcct tagacgttca
    31261 aggcaaatcc agcatcaccg actgcatgat catctgtacg ggtacgtcca gccgtcatgt
    31321 tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg
    31381 cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg tgattgtcca
    31441 tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa aaactctgga gttaatgcgt
    31501 gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa ccggttttac
    31561 cgagtacctg cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa ttccggccgg
    31621 aaaacgcggc aagaatgcgg acatcaagcg catactcgac aaagagggtg agcagatgtt
    31681 ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc cctgggatac
    31741 gccgcagtta gccgctgagc tggaacgctg gaagctggat ggtcgcgacg tcagtctact
    31801 gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg gcggcggagc agagctggtc
    31861 gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag agagtctgta
    31921 ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt gagtgataag ggagctttga
    31981 gtagaaaacg cagcggatga aactacagaa ctcttttcgc gactatacgg cagagtccgc
    32041 gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt ttgctgctga ccggcgtgct
    32101 tatcgccaac ctgtataatc tgcaaattgt tcgctttacc gactaccaga ctcgctctaa
    32161 tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc ggcattatct acgaccgtaa
    32221 tggtatccct ctggccctca accgcactat ctaccagata gaaatgatgc cagagaaagt
    32281 cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg gttgatctga ccgatgacga
    32341 tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa
    32401 aactaacctg accgaagtac aagtagctcg ctttgccgtc aatcagtacc gttttccggg
    32461 tgtcgaagtt aaaggctata aacgtcgtta ctatccttac ggttcggcgt tgacccacgt
    32521 catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata atgacggcaa
    32581 actggccaac tatgcggcaa cgcatgatat cggtaagctg ggcattgagc gttactatga
    32641 agacgtgctg cacggtcaga ccggttatga agaggttgaa gttaacaacc gtgggcgtgt
    32701 tatccgccag ttaaaagaag taccaccgca agccggacac gatatttacc tgacgctgga
    32761 tctcaaactc cagcaatata ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt
    32821 caccgatccg cgtacaggtg gggtgctggc gctggtttcc acgcctagtt atgacccaaa
    32881 cctgtttgtt gacggtatct ccagcaaaga ttattccgcc ttgttgaacg ccccgaatac
    32941 accgctggtg aaccgcgcca cacagggggt ttatcctccc gcgtctacag ttaaacccta
    33001 tgtggcggtt tcggcattga gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc
    33061 aggctggtgg caactgccag gttcggaaaa acgttatcgt gactggaaaa aatggggcca
    33121 cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg gataccttct tctatcaggt
    33181 ggcctacgat atggggatcg atcgcctctc cgaatggatg ggtaaattcg gttatggtca
    33241 ttacaccggt atcgacctgg cggaagaacg ttccggcaac atgcctaccc gcgaatggaa
    33301 acagaaacgc tttaaaaaac cgtggtatca gggtgacacc attccggttg gtatcggtca
    33361 gggttactgg acagcgaccc caatccagat gagtaaggca ctgatgatcc tgattaatga
    33421 cggtatcgtg aaggttcctc atttgctgat gagcaccgcc gaagacggca aacaggtgcc
    33481 atgggtacag ccgcatgaac cgcccgtcgg cgatattcat tccggttact gggagctggc
    33541 gaaagacggt atgtacggtg ttgctaaccg ccctaacggt acggcgcata aatactttgc
    33601 cagcgcaccg tacaaaattg cggcgaaatc cggtaccgct caggtcttcg gtctgaaagc
    33661 gaacgaaacc tataatgcgc acaaaattgc cgagcgttta cgtgaccaca aactgatgac
    33721 cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc atgattctgg agaacggtgg
    33781 tgcgggtccg gcggttggta cactgatgcg ccagatcctc gaccacatta tgctgggtga
    33841 taacaacacc gatctgcctg cggaaaatcc agcggttgcc gcagcggagg accattaatc
    33901 atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg
    33961 ctgctgatct tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt
    34021 caggatattg gcatgatgga gcgtaaaatc ggccaaatcg cgatgggtct agtcatcatg
    34081 gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct gggcccccta tctctatatc
    34141 atctgtatta ttttgctggt ggcggtagat gctttcggtg ccatctctaa aggtgctcaa
    34201 cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa aatagccgta
    34261 ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact
    34321 ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg
    34381 ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc
    34441 tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt tcattccgat tctgtggttc
    34501 ttcctgatgc atgattacca gcgccagcgc gtaatgatgc tcctagaccc ggaatcagac
    34561 ccactcggcg cgggctatca cattattcag tctaaaattg ctattggctc cggcggatta
    34621 cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc cgaacgccat
    34681 accgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg
    34741 ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc
    34801 acctttggtc gcgtcatggc tggtggctta atgctgatat tattcgttta tgtcttcgta
    34861 aatattggta tggtaagcgg tattctgccg gttgtagggg ttccgctccc actggtcagt
    34921 tatggaggat cggcgctaat tgtgctgatg gctgggttcg ggattgtaat gtcaatccac
    34981 acccacagga aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct
    35041 cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca
    35101 gacggtaagt gtaccgcagc ctgcggtatg taacggcccc atagttgaaa ttagcggggc
    35161 ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa
    35221 aagctacaaa atcgtgcagg atccgtctcg atttagccag gcgggactgg cggcaatcta
    35281 tgatgccgaa cccggcagta acctgacggc ctctggcgaa gctttcgatc cgacacagct
    35341 gacggcggcc catccaacgc ttccgatccc cagctacgcc agaatcacta acctggctaa
    35401 cgggcgaatg atcgtggtgc gcattaatga tcgcggccct tacggcaacg accgcgttat
    35461 ttcactttct cgcgcggcag ctgaccgtct taacacgtca aacaacacca aagttcgtat
    35521 cgatccgatt attgtcgccc aggatggttc gctttctggt cctggtatgg cttgtaccac
    35581 agtcgccaaa cagacttacg ccctgccagc acctcccgat ttaagcggtg gcgcgggaac
    35641 aagttcagtg tctggcccgc agggtgacat tcttccggtc agtaattcga cgctaaaaag
    35701 cgaagatccg accggcgcgc cggtaaccag tagcggtttc ctcggcgctc caacgacctt
    35761 agcgcctggt gtactggaag gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc
    35821 tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag
    35881 cgccagcggc aactttatgg tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca
    35941 gtaccaacag caactgggac agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc
    36001 ggtctggcgg atccagcttg gcccattcgc cagcaaagcc gaagccagta ccttgcagca
    36061 acgtttgcaa accgaagccc aattacagtc atttattact accgcgcagt agcgtaaagc
    36121 aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat
    36181 gcctgccggt agtgcatttg ctatagtagg gcactttttt aattccatca cggatgtcgt
    36241 agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac
    36301 ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata tcaaaactat
    36361 gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg
    36421 caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat
    36481 gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt
    36541 agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct
    36601 gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct
    36661 gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc
    36721 ttttgttggc ttgatgaaca gctacgttaa cgcactgggc ctgaaaaata cccacttcca
    36781 gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat
    36841 cggccaggca ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt
    36901 tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa
    36961 tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc
    37021 gactgaaggc cagatgcgct tgatttctgc ggtgatgggc ggacgtactt ttaaaggccg
    37081 tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc
    37141 actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt ctgatcgcgc
    37201 ttcgttaggg gttgataaag acgtgtacct gaccattcca cgtggtcgca tgaaagatct
    37261 gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt
    37321 cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc cgctggttgt
    37381 gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat
    37441 gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat
    37501 actaagcatc agtaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg
    37561 ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt
    37621 acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc
    37681 gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact
    37741 cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac
    37801 taggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggcccct
    37861 ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc
    37921 cacggagatg cagttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct
    37981 tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt
    38041 gatgaaatct ggctggtcga gcactatccg gtattcaccc aaggtcaggc aggaaaagcg
    38101 gagcacattt taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg
    38161 acttatcacg ggccggggca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa
    38221 ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa
    38281 ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa
    38341 atttgctcac tgggtttacg tattcgacgc ggttgttcat tccacggtct ggcattaaac
    38401 gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa
    38461 atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc accacgttta
    38521 ctggaaaata ttttagcgct actaaacaat cctgacttcg aatatattac cgcttaattg
    38581 catacctcaa tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac
    38641 atctccttcg cgtttgtata ctttctttat cacttgcaga aatgcggtgc tacgccttgc
    38701 tccgtaactc accccattga ttaacctatt gaaatagttg attatggtga gcaaaagcgt
    38761 tccgttccaa ttagacataa gattattgta atttttttaa atattatttt tccatgaata
    38821 aaattggagt aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc
    38881 gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac
    38941 tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag tgcttaatct
    39001 gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc ctgacccatt
    39061 gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc tacatgagta
    39121 tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa
    39181 gcaacgaacg ataactattg cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat
    39241 ctatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc ccatcagcga
    39301 cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcgataaca tgttttgcac
    39361 caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa tttgccgcga
    39421 gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg ctgcgcatgt
    39481 actcctgtta ccggaagggc aaaatttcag cggtctgcgg cagagagttc aagagatgtt
    39541 tccggaccgg caaatcaatt tcaccagcta caacattttg acaatcgctg cactggttgc
    39601 caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta gtcgttgctg
    39661 gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt tctctatcca
    39721 ctacaacaaa ttcagcctgc gtgatcccat cctgcacggg gtaatagatg ttatccgtaa
    39781 tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa aatggcacaa
    39841 tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga
    39901 tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat tgaattgaaa
    39961 cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc attgtgatgg
    40021 aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt aaaaacgtgg
    40081 caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg
    40141 actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg cattctgtct
    40201 gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca gcaacgttta
    40261 tgatcctcgg cgctatttgt acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc
    40321 cggtagctcc tgatgccaat gaaccagtga aactggcgca gaccattgcc gatatggcgc
    40381 tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc
    40441 actttgcgga ttgcattact gccattcggg aaaaaagccc gcaaatcaaa attgaaactc
    40501 tggtgccgga tttccgtggt cgtatggatc gtgctctgga tattctgact gcaacgccac
    40561 cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg
    40621 gtgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg catccggaaa
    40681 tcccgaccaa gtctggtctg atggtgggac tgggtgaaac caatgaagaa attattgagg
    40741 taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa tatttgcagc
    40801 caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc gacgaaatga
    40861 aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt gtccgctctt
    40921 cttaccacgc cgatttgcag gcgaaaggga tggaagttaa gtaatcctgt aataattact
    40981 tacatctgtc tgataaaaaa cccgcttttt g
//
LOCUS       DAQSWQ010000027        41003 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.027, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000027 DAQSWQ010000000
VERSION     DAQSWQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.027"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(200..1057)
                     /gene="murI"
                     /locus_tag="R8G18_001493"
     CDS             complement(200..1057)
                     /gene="murI"
                     /locus_tag="R8G18_001493"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEE9795228.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1002..2846)
                     /gene="btuB"
                     /locus_tag="R8G18_001494"
     CDS             complement(1002..2846)
                     /gene="btuB"
                     /locus_tag="R8G18_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HEE9795229.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPDTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2911..3101)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3215..4315
                     /gene="trmA"
                     /locus_tag="R8G18_001495"
     CDS             3215..4315
                     /gene="trmA"
                     /locus_tag="R8G18_001495"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEE9795230.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4355..4714)
                     /locus_tag="R8G18_001496"
     CDS             complement(4355..4714)
                     /locus_tag="R8G18_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HEE9795231.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4714..5364)
                     /gene="fabR"
                     /locus_tag="R8G18_001497"
     CDS             complement(4714..5364)
                     /gene="fabR"
                     /locus_tag="R8G18_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HEE9795232.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5685..7085
                     /gene="sthA"
                     /locus_tag="R8G18_001498"
     CDS             5685..7085
                     /gene="sthA"
                     /locus_tag="R8G18_001498"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEE9795233.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7068..7985)
                     /gene="oxyR"
                     /locus_tag="R8G18_001499"
     CDS             complement(7068..7985)
                     /gene="oxyR"
                     /locus_tag="R8G18_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HEE9795234.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(8252..9625)
                     /gene="argH"
                     /locus_tag="R8G18_001500"
     CDS             complement(8252..9625)
                     /gene="argH"
                     /locus_tag="R8G18_001500"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEE9795235.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9686..10462)
                     /gene="argB"
                     /locus_tag="R8G18_001501"
     CDS             complement(9686..10462)
                     /gene="argB"
                     /locus_tag="R8G18_001501"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEE9795236.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10470..11474)
                     /gene="argC"
                     /locus_tag="R8G18_001502"
     CDS             complement(10470..11474)
                     /gene="argC"
                     /locus_tag="R8G18_001502"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEE9795237.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11628..12779
                     /gene="argE"
                     /locus_tag="R8G18_001503"
     CDS             11628..12779
                     /gene="argE"
                     /locus_tag="R8G18_001503"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEE9795238.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13131..15782
                     /gene="ppc"
                     /locus_tag="R8G18_001504"
     CDS             13131..15782
                     /gene="ppc"
                     /locus_tag="R8G18_001504"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEE9795239.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15965..17698
                     /locus_tag="R8G18_001505"
     CDS             15965..17698
                     /locus_tag="R8G18_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HEE9795240.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17913..18764
                     /locus_tag="R8G18_001506"
     CDS             17913..18764
                     /locus_tag="R8G18_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9795241.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18751..19092)
                     /locus_tag="R8G18_001507"
     CDS             complement(18751..19092)
                     /locus_tag="R8G18_001507"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HEE9795242.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19094..19972)
                     /locus_tag="R8G18_001508"
     CDS             complement(19094..19972)
                     /locus_tag="R8G18_001508"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HEE9795243.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(19938..22234)
                     /locus_tag="R8G18_001509"
                     /pseudo
     CDS             complement(19938..22234)
                     /locus_tag="R8G18_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            complement(22285..22605)
                     /locus_tag="R8G18_001510"
                     /pseudo
     CDS             complement(22285..22605)
                     /locus_tag="R8G18_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            complement(22620..23699)
                     /locus_tag="R8G18_001511"
     CDS             complement(22620..23699)
                     /locus_tag="R8G18_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HEE9795244.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24008..26509
                     /gene="ptsP"
                     /locus_tag="R8G18_001512"
     CDS             24008..26509
                     /gene="ptsP"
                     /locus_tag="R8G18_001512"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEE9795245.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26521..27183
                     /gene="fsa"
                     /locus_tag="R8G18_001513"
     CDS             26521..27183
                     /gene="fsa"
                     /locus_tag="R8G18_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEE9795246.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27194..28297
                     /gene="gldA"
                     /locus_tag="R8G18_001514"
     CDS             27194..28297
                     /gene="gldA"
                     /locus_tag="R8G18_001514"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HEE9795247.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28571..29188
                     /locus_tag="R8G18_001515"
     CDS             28571..29188
                     /locus_tag="R8G18_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEE9795248.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29215..30120)
                     /gene="yijE"
                     /locus_tag="R8G18_001516"
     CDS             complement(29215..30120)
                     /gene="yijE"
                     /locus_tag="R8G18_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HEE9795249.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(30214..32394)
                     /gene="katG"
                     /locus_tag="R8G18_001517"
     CDS             complement(30214..32394)
                     /gene="katG"
                     /locus_tag="R8G18_001517"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEE9795250.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32723..33613)
                     /gene="metF"
                     /locus_tag="R8G18_001518"
     CDS             complement(32723..33613)
                     /gene="metF"
                     /locus_tag="R8G18_001518"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEE9795251.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33962..36394)
                     /gene="metL"
                     /locus_tag="R8G18_001519"
     CDS             complement(33962..36394)
                     /gene="metL"
                     /locus_tag="R8G18_001519"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HEE9795252.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36397..37557)
                     /gene="metB"
                     /locus_tag="R8G18_001520"
     CDS             complement(36397..37557)
                     /gene="metB"
                     /locus_tag="R8G18_001520"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEE9795253.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37834..38151
                     /gene="metJ"
                     /locus_tag="R8G18_001521"
     CDS             37834..38151
                     /gene="metJ"
                     /locus_tag="R8G18_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HEE9795254.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38335..38943
                     /locus_tag="R8G18_001522"
     CDS             38335..38943
                     /locus_tag="R8G18_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HEE9795255.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39088..39763
                     /locus_tag="R8G18_001523"
                     /pseudo
     CDS             39088..39763
                     /locus_tag="R8G18_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39936..40172)
                     /locus_tag="R8G18_001524"
     CDS             complement(39936..40172)
                     /locus_tag="R8G18_001524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795256.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(40174..>41003)
                     /locus_tag="R8G18_001525"
     CDS             complement(40174..>41003)
                     /locus_tag="R8G18_001525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HEE9795257.1"
                     /translation="GRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDG
                     LEIRREYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQD
                     PIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGG
                     LICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKEN
                     DVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT        10045 a        11260 c        10216 g         9482 t
ORIGIN      
        1 ggtgtgcata atacgctttc ccgctacaga gtcaagcatt ttttttgctt ttctctgttg
       61 agattctcag gagaaccccg ctgacccggc ggcgtgtttg ccgttgttcc gtgtcagtgg
      121 tggcgcatta tagggagtta ttccggcctg acaagaggaa atttaaaata attttctgac
      181 cgcgcaacat tcaaccaaat cagcctaaaa ctgccagttt ttcgagcgtt tcgaagccgt
      241 aacgctgtaa aacgggcaat aattgttcag cttctggcgt cattgccata caaaaggcaa
      301 tattcgcatc ggcagatttt gcatccgggg cttcatgttc taacaaccag gccgttcggc
      361 gagcaatcgc tgcgccagaa tccaccagcc gggttccctc tggcagcact tgtaacagtt
      421 cttcttgtag tagagggaaa tgggtgcaac ccaatacaac ggtatctggc ggctctttca
      481 ttcttaacca cgggcgtagg atacgtttta gtgcatccag agaaacatct tcgccatgta
      541 gcttcgcttc agccaactca accatctctg ccgagcccag catttctatc tggcattcat
      601 tagcgaaacg cgcgatcagc tcatgagtat aagaacgttt aactgttccg cgggttgcca
      661 gtaatccgac aatgccattt gccgtcagac gtgcagcagg tttaatcgcc ggcacgacac
      721 caacaaccgg gaagtcgaac ttttcgcgta atgcaggaag tgaaacggta ctggcagtgt
      781 tgcaagcgac cacagccagc gcaaggggat aacgttcttg caccgcggtg acaattgcca
      841 ccactcgctc aacaataaac gcttcgcttt tttcgccata cgggaaagcg acgttatcga
      901 aagcataaat gtaatggaga tccggtaaga gatgccggat ctcgtcatag accgacaacc
      961 caccgacgcc ggagtcaaac accagcacgg tgggacgtgg ttcagaaggt gtagctgcca
     1021 gacaaggtgt attcccgtcc tgcagtttgg tagccataga ctgtctcata atctttgtcg
     1081 aacaggttgg ctattttacc acgaactgtc aggtgagagg tgtccggata cgcaaccgca
     1141 agatcccaca agctcacacc gcccatttta acggtttgat aaggataaga tgagtaatcc
     1201 ttatcatagc gagtgcctaa atactgataa gtaatacccc agtcgaagtc atacaactgc
     1261 cagtcgagct ggtatttcac ctgctgttta gcacggcgta acaacggcgt gtcggtaatc
     1321 gcattgcgcg catcgacata atcataactc acagtatgcg tcagtggtcc ggtatcaaaa
     1381 ttggcggtcg cctcgacgcc cttaatccgc gctttccctt cgttgtaata tttcagggtg
     1441 tgatcatcat aatcgatcaa gtcactgaca tcgttacgat atccggaaat acgccagttc
     1501 accccagcgg ttaagccttc aaacgcgcct tcccactgtt tgcttttctc cgggtccaga
     1561 ttcggatttc cgtagaagcc atacagttgc cccagatttg gtgccttata agatgtcccg
     1621 taggaagcaa tgaagcgata accttcgatg aattcccaac cggcgctggt ttgccaggtt
     1681 ccatgacgac caaactgtga gttatcgtca ctgcgtgctg cgccttcaaa ggtaaaatcg
     1741 ccgacttgtt gcagcccggt cagatagatg ccggtattac gttgatcata tccatcctca
     1801 acataacctg tacccggcgt cgtagtctgt ttctgccagt cgacacccgc accaatacta
     1861 ccgtgaccaa cgatgacatt gtttgcccac tggacggtgt attgcttcat ctcatcgagc
     1921 gtcgccgacg aatcataacg accataatgg ggatcgtagt tgtaatcttt gctatggcta
     1981 tagctggtaa tgagttgtga tttaatcagt tcgccgttat agcgcagccc ggcgtcccaa
     2041 ctttggctat agagtttacg ggtatcgagc aacggtgaac cgggagaata atacgcgtca
     2101 taattggtac ggttatcata gccatagccg cgcacaaagc cgctccaggc atcagtaaag
     2161 ttatgctcca gcgcgccata aagcgtttta cttaaaaaac catcgttatc tgtctgcgct
     2221 tgcgttcctg tattaccata ggcaacaaca tcataaccat gagtatgggc ataatcgccc
     2281 aacagcgtta cccgtgtctt atcccccagt tgttgctgcg tagagacatc atagttctga
     2341 taactattgc ttccccaccc tgctgaaatt tccgttccgg gttcatcgcg cgtcgtgatg
     2401 atattcacca ccccgcctat tgcatcggaa ccataaacag cggagcgcgg cccacggata
     2461 tattcaacac gctggacaag cgcaataggg aactggctaa ggtcggcaga accactcacc
     2521 cccgccagat tcaggcgtac gccatcaatt aacaccaaca catgactggc atttgtaccg
     2581 cgaataaaaa tagatgagag ctgacctgaa ccgccgtttt gggtgatatc gacgcccgga
     2641 agacggcgca gcacatcatt gaccgaggtc gactgccagc ggtcgatatc ctgacgggtc
     2701 acaacggtgg ttggtgcaag cacagtgctg cgcggctgtt caaaacggtt agcagtaacg
     2761 acgagagtat ccgggctggt atcctgtgcc caagcggaaa aggctgtgac agaacacgcc
     2821 gtcagcagcg aagctttttt aatcattgta aagcatccac aatagaagaa ggatgccgca
     2881 ggtttcatca atattacgcg atgatgagaa ccagatgcga cgttggccgg caggtcttcg
     2941 ggcttggagg ggtatctaag atactaagag atgatgactt cccaccggat ggcagtgtcc
     3001 gcataacgca atcatcgcac ctttccttac cgctgcgcgt cagctccaga ttcgcactgg
     3061 attccctatt aactcacagg accgacaagt ggatgctaca ggttgtaaca agttactgtc
     3121 cagacgtagc tcacaaatag gaattcatca agatctggac atctgatgag caatccctac
     3181 aatcgccgcg tactttaatt tttcaggata catcatgacc cccgaacacc ttccaacaga
     3241 acagtatgaa gcgcagttag ccgaaaaagt ggtacgtttg caaagtatga tggcaccgtt
     3301 ttctgacctg gttccggaag tgtttcgctc gccggtcagt cattaccgga tgcgtgcgga
     3361 gttccgcatc tggcacgatg gcgatgacct gtatcacatc attttcgatc aacaaaccaa
     3421 aagccgtatc cgcgtggata gcttccccgc cgccagtgaa cttatcaacc agttgatgac
     3481 ggcgatgatt gcgggtgtgc gtaataatcc cgttctgcgc cacaagttgt tccagattga
     3541 ttacctcacc acgctgagta atcaggcggt ggtttccctg ctataccata agaagctgga
     3601 tgatgagtgg cgtcaggaag cggaggccct gcgcgatgca ctgcgcgcgc agaatctgaa
     3661 tgtgcatctg attggtcggg caacgaaaac caaaatcgag ctggatcaag attacatcga
     3721 cgagcgtctg ccggtcgcag ggaaagagat gatctaccgt caggtagaaa acagctttac
     3781 ccagccgaac gcggcgatga atattcagat gctggaatgg gcgctggacg taaccaaagg
     3841 ctcaaaaggc gatttactgg agctgtactg cggcaacggt aacttttcat tagcgctggc
     3901 gcgcaatttt gatcgggtat tagccaccga aatcgctaag ccgtcggttg ctgctgcgca
     3961 atacaacatc gcagctaacc atattgataa cgtacaaatt attcgtatgg cggcagaaga
     4021 atttactcag gcgatgaatg gtgtgcgcga gtttaaccgc ctgcaaggga tcgacttaaa
     4081 gagttatcag tgcgaaacca tttttgtcga cccaccgcgc agcggtctgg acagtgaaac
     4141 cgagaaaatg gtgcaggcgt atccgtgtat tttgtacatc tcctgtaacc cggaaacgtt
     4201 atgcaagaat ctggaaacat taagccagac gcacaaggtc gaacgtctgg ctctgtttga
     4261 tcagttcccc tacacgcacc atatggagtg cggcgtatta ctgaccgcga agtaaaaccc
     4321 atgccggatg cgccagcatc cggcataata ccgattactc agcaacttcc tgcttacggt
     4381 tacgcatctt cgcgccaatc cagaacacca taatgacgga gagtactgcc gggaagaagt
     4441 tagagccgat atccggatat tccgcacgaa ctaccgtgct gtacagcagt acgccgagaa
     4501 taaaacaggc agctgccaga cccggcaagc ctaccggcat agtgcgatta agataacgtt
     4561 gatgcaggca gtaaaccgtc agcaccaggg aaataatcgg gaatacagaa aatggcacaa
     4621 tggagctaaa cagcgctgcg aaagtaccat taatcgataa gccagcaact aacgccagca
     4681 gcagcgtacc tctatcttga tttgcttgtt tcattactcg tccttcacat ttcccggaat
     4741 aattgcggtt ttctcttgtt cacggcgata ccagtaataa gcccctttcg aaatcattcg
     4801 cagttgcagt accagtcgct cttctaattg ccgacgttgt tcgacgccga cgtccaacgc
     4861 ctcggcaccc gcactgaaga caattgtcac cattgcttcg gcttgcgctt cagtaaacgc
     4921 acgcggcata tggttttcga gttccagata gtccgcaagt tccgcaatga agtgctgaat
     4981 ttcacgcgca acggcggcac gaaacgcagc ggaggtgccg gagcgttccc gcaataataa
     5041 ccggaaggcg ttaggattat taccgatgaa ctccataaat gtggagaccg aggtgcggat
     5101 cacactcccg cctttggcga tacgctgacg cgcctggcgc atgagttggc gtagcattaa
     5161 accgctctca tcaaccatgg tcagacccag ttcgtctacg tcgcggaaat gccgataaaa
     5221 agaggtggga gcaatgcccg cttcacgcgc cacttcacgc aaactcaggc tggcgaagct
     5281 gcgttcagca cttaattggc taaatgcggc ttccaccagc gaacggcggg ttttttcttt
     5341 ttgttgcgct cttatgccca tcacgatgtc tgaatccttg ccaaaagtac tgctggcact
     5401 ataccagaga atgaacatac tggattgctc cagtattgtg aatgaacggt aacgcaataa
     5461 aacatattta ctggccggtt cttttacgta cagcggaaac ctgccgctta aacggagagt
     5521 atcgtcgata aaaatccaat aaaacgtcag ggcaaaagta agaaacagac aaagcaaagg
     5581 ccgctcagga tatagccaga taaatgacgg ggatcaattg gcttacccgc gatagaatgt
     5641 taccattctg ttgcttttat gtataagaac aggtaagccc taccatgcca cattcctacg
     5701 attacgatgc catagtaata ggttccggcc ccggcggcga aggcgctgca atgggcctgg
     5761 ttaagcaagg tgtgcgcgtc gcagttatcg agcgttatca aaatgttggc ggcggttgca
     5821 cccactgggg caccatcccg tcgaaagctc tccgtcacgc cgtcagccgc attatagaat
     5881 tcaatcaaaa cccactatac agcgaccatt cgcgactgct ccgctcttct tttgccgata
     5941 tccttaacca tgccgataac gtgattaatc aacaaacgcg catgcgtcag ggattttacg
     6001 aacgtaatca ctgtgaaata ttgcagggga acgctcgctt tgttgacgag catacgttgg
     6061 cgctggattg cccggacggc agcgtcgaaa cactaaccgc tgaaaaattt gttattgcct
     6121 gcggctctcg tccatatcat ccaacagatg ttgatttcac ccatccacgc atttacgaca
     6181 gcgactcaat tcttagcatg caccacgaac cgcgccatgt acttatctat ggtgctggag
     6241 tgatcggctg tgaatatgcg tcgatcttcc gcggtatgga tgtaaaagtg gatctgatca
     6301 acacccgcga tcgcctgctg gcatttctcg atcaagagat gtcagattct ctctcctatc
     6361 acttctggaa cagtggcgta gtgattcgtc acaacgaaga gtacgagaag atcgaaggct
     6421 gtgacgacgg tgtgatcatg catctgaagt cgggtaaaaa actgaaagct gactgcctgc
     6481 tctatgccaa cggtcgtacc ggtaataccg attcgctggc gttacagaac attgggctgg
     6541 aaactgacag tcgcggacag ctgaaggtca acagcatgta tcagaccgca cagccgcacg
     6601 tttacgcggt gggcgacgtg attggttatc cgagcctggc atcagcggcc tatgaccagg
     6661 ggcgcattgc cgcgcaggcg ctggtaaaag gcgaagccac cgcgcatctg attgaagata
     6721 tccctaccgg catttacacc atcccggaaa tcagctctgt gggcaaaacc gaacagcagc
     6781 tgaccgcgat gaaagtgcca tatgaagtgg gccgcgccca gtttaaacat ctggcacgcg
     6841 cacaaatcgt cggcatgaac gtgggcacgc tgaaaatttt gttccatcgg gaaacaaaag
     6901 agattctggg tattcactgc tttggcgagc gcgctgccga aattattcat atcggtcagg
     6961 cgattatgga acagaaaggt ggcggcaaca ctattgagta cttcgtcaac accaccttta
     7021 actacccgac catggcggaa gcctatcggg tagctgcgtt aaacggttta aaccgcctgt
     7081 tttaacactt tatcgaaata gccatccatt cttgctcgga tggcctctgc cagctgctca
     7141 tagcggctgc gcagcggtga gccaggacga taaaccaggc caatagtgcg gcgtggttcc
     7201 ggcttaatgc acggcagata aacaacccca tcgcgtttgc gctccggcgg cacagccagc
     7261 gctggcagta aagtgatccc gctacctgcc gccaccatgt tgcgcagcgt ctccaggctg
     7321 gtcgcgcgga agtgtgtatc ttcatccgcc ccggcttcaa aacagaaacc cattgcctga
     7381 tcgcgcaaac agtgaccatc ttccagcatc agcagttttt cccctgccag atcggccatc
     7441 ggtacgcatt cgcggttcgc ccacgggtga tcttcataga tagccagcaa cattggctca
     7501 tcaaacaacg gcacttcaat gaatgcttcg ctctctttca ccagcgcgag gatcacgcaa
     7561 tcgagtttgc cactgtccag ttgcgccagt aactggtggg tctgtgcttc atgcagatac
     7621 atttccagct ttggaaaggt ctggtgcagc atcgggataa tatgcggtag caggtacggt
     7681 ccaaccgtgg gaatcaaacc aatatgcagc ggtccggaca tcgtctcgcc ctgctggctt
     7741 gccatctctt taaggacttt cacctcacgc agcacggtac gcgcctgatc caccagcagc
     7801 attcccgcct gggtgaacaa cactttacgg ctggtccgct ccagcaacat cacgcccagc
     7861 tcatcttcca gcttacgaat ttgcccgcta agcgtcggct ggctaacgtg gcaggaatct
     7921 gccgcacgcc gaaaatggcg gtgttcagcc aatgccacca ggtactcaag atcacgaata
     7981 ttcattatcc atcctccatc gccacgatag ttcatggcga taggtagaat agcaatgaac
     8041 gattatccct atcaagcatt ctgactgata attgctcaca gaaacggagc ggcacctctt
     8101 ttaacccttg aagtcactgc ccgtttcgag agtttctcaa ctcgaataac taaagccaac
     8161 gtgaactttt gcggatctcc aggatccgct tttttttgcc ataaaaaagc ccggcgataa
     8221 gccaggctca aatttgtaca tataaatgtt cttaccccaa ccgcgcctgc gcaaaagcaa
     8281 tcgcttgggc cacctgctgc ggtgagacgc cgccttttgc cgcacgcttg tcgaggcacg
     8341 attgcagcga caaaatcgga tagacatctt cgccaatcac cgggctgaat ttctgcaact
     8401 cgtcgagcgg cagatcttcc agcggtttgc cctgacgaat ggcttccacc accgcttcac
     8461 ccacgatatg gtgcgcctcg cggaaaggta cgcctttcgc caccaggtaa tccgccagtt
     8521 cggtggcgtt ggcgtaaccc tgctgagccg cttcctggca acgtggacgt ttcacctgaa
     8581 tgccgtccag caccagcgcc gccatatgca ggcagtccag ccaggtatcg agcgcgtcga
     8641 acagaccttc tttgtcttcc tgcatatctt tgttgtaagc cagcggcaaa cctttcagcg
     8701 tcatcatcat gccggttaac gcaccctgca cccgaccgca tttaccgcga atcagctcca
     8761 gcgcatccgg gtttttcttc tgcggcatta atgatgaacc ggaagtcacg cggtcagaaa
     8821 gctccacaaa ccccgcttcg ccggtgttaa agaaaatcag atcttcagca aaacgcgaca
     8881 gatgcaccat gccgatagcg gcagccgaca gcagttccaa cacatggtca cggtcagaaa
     8941 cgctgtccag actgttacgg gtcgccgaag caaagcccag ccagcctgct aactgttcac
     9001 ggtcgatttc ataggccgtt cccgccagcg cgccacagcc taatgggctg acatccagac
     9061 gcttaagcgc atcctgcaaa cggctttcat cacgcgccag catctcaaca taggccaggc
     9121 accagtgcgc gaacgtcacc ggctgggcgc gttgcaggtg agtgtaacct ggcatgaccg
     9181 cgtcctgatt gttttgtgcg gtttccacca gcgccgattg cagctgtcgg ttagccgtca
     9241 gtaactcgct aacggtatct ttgcaccaca gtttcaggtc agtcgctacc tgatcgttac
     9301 gactacgccc ggtatgcagc tttttgccta actggcccac tttgtcgatc agtttgcctt
     9361 ccacccagct atggatatct tcggcgtcgc tttcaaggat ttgttgtggc ctggcgcgaa
     9421 catcttccag cagcacgttc agcgcctctt ccagttgcgc ctgctcttct gcggttaaca
     9481 cgcctaccgt gaccagggct ttggaccagg ccacagagcc aacaatatcc tgctccgcca
     9541 gacggtaatc aaagcgcagt gagtcgttga attgtttgaa ccgttgatct gctgcctggg
     9601 taaaacgccc gccccaaagt gccataactc tgtttcctta ttttgaaatt caatgccgga
     9661 aagctgcgcc tccggccaac aaaacttaag ctaaaatccg cgtacccatc ggcataccgt
     9721 taaacagtgc cggaagctgc tccgcatgac gccaggaggc gatatctacc ggacggccca
     9781 gcgtgcgggc cgcatccagc gccgcgttca ctttcactat catgccgtca gtaataatgc
     9841 cctgctcaat cagttgttct gctttcgcgg cggtcatttc ggcaatgcgt tgccctttgc
     9901 cgtcgagaat gccgctgacg tcggagagca aaatcagatc cgcgcccagc gttgccgcca
     9961 gcgccgttgc cgcctggtcg gcattgacgt tcatcagttg cccttcgtct gttacgccaa
    10021 tggagctgac caccggcaga taaccgttct ccagcaagga gttgataagc ttaggcgaac
    10081 ctggctgcgc cagtccaaca tgacctaact cttcatcaag ctgggtcact ttgacgctgt
    10141 cgccgtcacc gagaaacaaa cctacggccg caatctgatg tttcttcgcc catgccaaca
    10201 gggttttatt tgccgttccc gccagtgctc cggtgataat gtctatctga tcagcaggcg
    10261 tcacccgcag gccgtttttc tttttcaccg gcagattcag ccctttcatc agctcatcca
    10321 ccacgcaacc gccgccgtgc acaatcacca gcggacgctg atgtgactca cgataattca
    10381 ccagtgtgct aaacagacgt tccagcgcct cttcactatc cagcagtacg ccgcccagtt
    10441 tgataattaa tggattcatc attgcaccct taaataagag actgcgtttc cgcatagccg
    10501 aaacgaatat tggcgcactg taccgcttgt gccgccgcgc ctttcagtaa gttgtcttcg
    10561 gtcgccacaa taatcagatg ctcgccctga acggcaaacc cgatatcgca aaatggcagc
    10621 ccaacgacat ttttcagcgc cggaacgcct ttgtcataca gccgcaccag cggtttatgg
    10681 gcatacgcct gctgtaacac ttgcgcgact tgcgcctggg tcacacccga tttcaggcgg
    10741 caggtaatgg tttcgagaat gccgcgcggg aaattgccca gatgtggggt gaagataacg
    10801 tcagcaccga ggtgtgtggc gatctctggt tgatggcgat gagtaaagac gccatacggt
    10861 tgcaggctaa cttcacaaaa actatttgaa atcgctgcct tacgtcctgc accgctcacg
    10921 ccgctggtgg cgttgatcac cggccactga ttgaggtcaa gaagatcggc atcaatcaac
    10981 ggtttcagcg ccagctgtgc cgccgtcgga taacagcctg gcaccgcaat caaattcgct
    11041 tcttttaatt tattaccgca ccactccgcc agaccgtagg ctgcctgttc cagcagttcc
    11101 gggtattgat gggtaaagcc gtaatatttt tcatagaagg tggcgtcgtt aacacgaaac
    11161 gcaccggaaa ggtcgaacac cacgcagccc gcttcaagaa actgcggcgc taaatcgtgg
    11221 ctaacttcat gggcggtggc gagaaacact acgtccaccc ctgggctaaa ctcgctgata
    11281 tccgacatcg gctgcaacgg cagctcaacg atgcctttta gctgcggatg caaatcggag
    11341 attaactttc ccgcatcatt gctttgcgct gaaacagtca aagcggttat gttcatatgc
    11401 ggatggcgat ttacataggt cactagctct gcgccagcgt agccgctggc acccacaatc
    11461 agcgtattca acatcggggc tattcacctt cttatgtctg gttgccaggt taaacgtaaa
    11521 acattcacct tacggctggt gggttttatt acgctcaacg ttagtgtatt tttattcata
    11581 aatactgcat gaatattgat actatcatga ccagaggtgt gtcaacaatg aaaaacaaat
    11641 taccgccatt tatcgagatt taccgtgctc tgattgccac accttcaata agcgccacgg
    11701 aagaggcact cgatcaaagc aatgcagatt taatcactct gctggcggac tggtttaaag
    11761 atttaggctt caatgtggaa gtgcagcctg tgccaggaac tcgcaacaaa ttcaatatgc
    11821 tggcaagtat cggacagggg gctggcggct tgttgctggc ggggcatacc gatacggtgc
    11881 catttgatga cggtcgctgg acgcgcgatc cgtttacact gacggagcat gacggcaagc
    11941 tttacggctt aggcaccgcc gacatgaaag gcttttttgc gtttatcctt gatacgctac
    12001 gcgatgtcga cgtcacgaaa ctgaaaaaac cgctctacat tctggcgact gctgatgaag
    12061 aaaccagtat ggccggagcg cgttattttg ccgaaactac cgccctgcgc ccggattgcg
    12121 ccatcattgg cgaaccgacg tcactacaac cggtacgcgc acataaaggt catatctcta
    12181 acgccatccg tattcagggc cagtcggggc actccagcga tccagcacgc ggagttaacg
    12241 ctatcgaact aatgcacgac gccatcgggc atattttgca attgcgcgat aacctgaaag
    12301 aacgttatca ctacgaagca tttaccgtgc cataccctac gctcaacctc gggcatattc
    12361 acggtggcga cgcttctaac cgtatttgcg cttgctgtga gttgcatatg gatattcgtc
    12421 cgctgcctgg catgacactc aatgaactta atggtttgct caacgatgca ttggctccgg
    12481 tgagcgaacg ctggccgggt cgtctgacgg tcgacgagct gcatccgccg atccctggct
    12541 atgaatgccc accgaatcat caactggttg aagtggttga gaaattgctc ggagcaaaaa
    12601 ccgaagtggt gaattactgt accgaagcgc cgtttattca aacgttatgc ccgacgctgg
    12661 tgctcggtcc gggttccatc aatcaagctc atcagcctga tgaatatctg gagacgcggt
    12721 ttattaaacc cacccgtgag ttgattatcc aggtaattca ccatttttgc tggcattaaa
    12781 acgtaggccg gataaggcgc tcgcgccgca tccggcaccg ttgccaaact ccggtgccgc
    12841 aataatgtcg gatgcgatgc ttgcgcatct tatccgacct acacctttgg tgttatgtat
    12901 ggcgattttt taacatttcc ataagttacg cttatttaaa gcgtcgtgaa tttaatggcg
    12961 taaattcctg ctatttattc gtttgctgaa gcgatttcgc agcatttgac gtcaccgctt
    13021 ttacgtggct ttataaaaga cgacgaaaag caaagcccga gcatattcgc gccaatgcga
    13081 cgtgaaggat acagggctat caaacgataa gatggggtgt ctggggtaat atgaacgaac
    13141 aatattccgc attgcgtagt aatgtcagta tgctcggcaa agtgctggga gaaaccatca
    13201 aggatgcgtt gggagaacac attcttgaac gcgtagaaac tatccgtaag ttgtcgaaat
    13261 cttcacgcgc tggcaatgat gctaaccgcc aggagttgct caccacctta caaaatttgt
    13321 cgaacgacga gctgctgccc gttgcgcgtg cgtttagtca gttcctgaac ctagccaaca
    13381 ccgccgagca ataccacagc atttcgccga aaggcgaagc tgccagcaac ccggaagtga
    13441 tcgcccgcac cctgcgcaaa ctgaaaaacc agccggaact gagcgaagac accatcaaaa
    13501 aagcagtgga atcgctgtcg ctggaactgg tcctcacggc tcacccaacc gaaattaccc
    13561 gtcgtacact gatccacaaa atggtggaag tgaacgcctg cttaaaacag ctcgataaca
    13621 aagatatcgc tgactacgaa cacaaccagc tgatgcgtcg cctgcgccag ttaatcgccc
    13681 agtcatggca tactgatgaa atccgtaagc tgcgtccaag cccggtagat gaagccaaat
    13741 ggggctttgc cgtagtagaa aacagcctgt ggcaaggcgt accgaattac ttgcgcgaac
    13801 tgaacgaaca actggaagag aacctcggct acaaactgcc cgtcgaattt gttccggtcc
    13861 gttttacttc gtggatgggc ggtgaccgcg acggcaaccc gaacgtcact gccgatatca
    13921 cccgccacgt cctgctactc agtcgctgga aagccaccga tctgttcctg aaagatattc
    13981 aggttctggt ttctgaactg tcgatggttg aagcgacccc tgaactgctg gcgctggttg
    14041 gcgaagaagg tgccgcagaa ccgtatcgct atctgatgaa aaacctgcgt tctcgcctga
    14101 tggcgacaca ggcatggctg gaagcgcgcc tgaaaggcga agaactgcca aaaccagaag
    14161 ggctgctgac acaaaacgaa gaactgtggg aaccgctcta cgcttgctac cagtcacttc
    14221 aggcgtgtgg catgggtatt atcgccaacg gcgatctgct cgacaccctg cgccgcgtga
    14281 aatgtttcgg cgtaccgctg gtccgtattg atatccgtca ggagagcacc cgccataccg
    14341 aagcgctggg cgagctgacc cgctacctcg gtatcggcga ctacgaaagc tggtcagagg
    14401 ccgacaaaca ggcgttcctg atccgcgaac tgaactccaa acgtccgctt ctgccgcgca
    14461 actggcaacc aagcgccgaa acgcgcgaag tgctcgatac ctgccaggtg attgccgaag
    14521 cgccgcaagg ctccattgcc gcctacgtga tctcgatggc gaaaacgccg tccgacgtac
    14581 tggctgtcca cctgctgctg aaagaagcgg gtatcgggtt tgcgatgccg gttgctccgc
    14641 tgtttgaaac cctcgatgac ctgaacaacg ccaacgatgt catgacccag ctgctcaata
    14701 tcgactggta tcgcggcctg attcagggca aacagatggt gatgattggc tattccgact
    14761 cagcaaaaga tgcgggcgtg atggcagctt cctgggcgca atatcaggca caggatgcat
    14821 taatcaaaac ctgcgaaaaa gcgggtattg agctgacgtt gttccacggt cgcggcggtt
    14881 ccattggtcg cggcggcgca cctgcccatg cggcgctgct gtcacaaccg ccaggaagcc
    14941 tgaaaggtgg cctgcgcgtg actgaacagg gcgagatgat ccgctttaaa tatggtctgc
    15001 cagaaatcac cgtcagcagc ctgtcgcttt acaccggggc gattctggaa gccaacctgc
    15061 tgccaccacc tgagccgaaa gagagctggc gtcgcattat ggatgaactg tcagtcatct
    15121 cctgcgatct ctaccgtggc tacgtacgtg aaaacaaaga ttttgtgcct tacttccgct
    15181 ccgctacgcc ggaacaagaa ttgggcaaac tgccgttggg ttcacgtccg gcgaaacgtc
    15241 gcccaaccgg cggcgtcgag tcgctgcgcg ccataccgtg gatttttgcc tggacgcaaa
    15301 accgcctgat gctccccgcc tggctgggtg caggtacggc gctgcaaaaa gtggtcgaag
    15361 atggcaaaca gagcgagctg gaagccatgt gccgcgattg gccattcttc tcgacgcgtc
    15421 tcggcatgct ggagatggtc ttcgccaaag cagacctgtg gttggcggaa tactatgatc
    15481 aacgtctggt agacaaagca ctgtggccgt taggtaaaga gttacgcaac ctgcaagaag
    15541 aagacatcaa agtggtgctg gcgattgcca acgattccca tctgatggcc gatctgccgt
    15601 ggattgcaga gtctattcag ctacggaata tttacaccga cccgctgaac gtattgcagg
    15661 ccgagttgct gcaccgctcc cgccaggcag aaaaagaagg ccaggaaccg gatcctcgcg
    15721 tcgaacaagc gttaatggtc actattgccg ggattgcggc aggtatgcgt aataccggct
    15781 aatcttcctc ttctgcaaac cctcgtgctt ttgcgcgagg gttttctgaa atacttctgt
    15841 tctaacaccc tcgttttcaa tatatttctg tctgcatttt attcaaattc tgaatatacc
    15901 ttcagatatc cttaaggaat tgtcgttaca ttcggcgata tttttttcaa gacaggttct
    15961 tactatgcat tccacagaag tccaggctaa accccttttt agctggaaag ccctgggttg
    16021 ggcactgctc tacttttggt ttttctctac tctgctacag gccattattt acatcagtgg
    16081 ttatagtggc accaacggca ttcgcgactc gctgttattc agctcgctgt ggttgatccc
    16141 ggtattcctc tttccgaagc ggattaaaat tattgccgca gtaatcggcg tggtgctatg
    16201 ggcggcctct ctggcggcgc tatgctacta cgtcatctac gggcaggagt tctcgcagag
    16261 cgttctgttt gtgatgttcg aaaccaacac caacgaagcc agcgagtatt taagccagta
    16321 tttcagcctg aaaattgtgc ttatcgcgct ggcctatacg gcggtggcag ttctgctgtg
    16381 gacacgcctg cgcccggtct atattccaaa gccgtggcgt tatgttgtct cttttgccct
    16441 gctttatggc ttgattctgc atccgatcgc catgaatacg tttatcaaaa acaagccgtt
    16501 tgagaaaacg ttggataacc tggcctcgcg tatggagcct gccgcaccgt ggcaattcct
    16561 gaccggctat tatcagtatc gtcagcaact aaactcgcta acaaagttac tgaatgaaaa
    16621 taatgccttg ccgccactgg ctaatttcaa agatgaatcg ggtaacgaac cgcgcacttt
    16681 agtgctggtg attggcgagt cgacccagcg cggacgcatg agtctgtacg gttatccgcg
    16741 tgaaaccacg ccggagctgg atgcgctgca taaaaccgat ccgaatctga ccgtgtttaa
    16801 taacgtagtt acgtctcgtc cgtacaccat tgaaatcctg caacaggcgc tgacctttgc
    16861 caatgaaaag aacccggatc tgtatctgac gcagccgtcg ctgatgaaca tgatgaaaca
    16921 ggcgggttat aaaaccttct ggatcaccaa ccagcagacg atgaccgccc gcaataccat
    16981 gctgacggta ttttcgcgcc agaccgacaa gcagtactac atgaaccagc aacgtacgca
    17041 gagtgcgcgt gaatacgaca ccaacgtgct gaagccgttc caggatgtgc tgaatgaccc
    17101 tgcgccgaag aaactgatca tcgttcatct gctgggtacg catatcaaat acaaataccg
    17161 ctacccggaa aatcagggca agtttgatgg caataccgat catgttccgc caggattaag
    17221 cgcagaagag ctggaatcat ataacgatta tgacaacgct aacctgtata acgatcatgt
    17281 ggttgccagc ctgattaaag actttaaagc ggcagacccg aacggattcc tggtttactt
    17341 ctctgaccac ggtgaagagg tttacgacac gccgccgcac aaaactcagg ggcgtaacga
    17401 agacaacccg acgcgccaca tgtacaccat tccgttcctg ctgtggacgt cggaaaaatg
    17461 gcaagcgact catccccgtg atttctcaca ggatgtcgat cgtaaataca gcctggcgga
    17521 actgatccac acctggtcag atttggcggg cttatcttac gacggttacg atccaacccg
    17581 ttcagtggtg aatccgcagt tcaaagaaac tacccgctgg attggtaacc cgtacaagaa
    17641 aaacgcgctg atcgattacg acactctgcc gtatggcgac caggtaggta atcagtaatt
    17701 atcagccagg cgctatttgc cggatgcgac gcttgacgcg tcttatccgg cctacagatc
    17761 gcggcggtag gctggataag gcgtttacgc cacatccggc aatacaactg ccgcaatgtt
    17821 tacatcgaac tcccctcagg ccacactttt caggtggcct tttttaataa tgccagttac
    17881 tgttttcccg tctacatacc taagggaata acatgtatca cgacgtcagc tatctgctct
    17941 cccgcctgat caacggcccg ctgtccctgc gccagattta ctttgccagc agtaacggcc
    18001 cggtgcccga tcttgcgtat caggtcgatt ttccacggct ggaaattgtg ctggaaggtg
    18061 agtttgttga taccggcgct ggagcaacgt tagttcccgg cgatgtgctg tacgttgccg
    18121 ctggtggctg gaatttccca caatggaaaa cacccgctac cacctttagc gtgctgtttg
    18181 gcaaacagca actcggcttc agcgtcgtgc aatgggatgg caaacaatat caaaatctgg
    18241 cgaagcaaca cgtcgcccga cgcggcccac gtataggttc ttttctgcta caaacgctca
    18301 atgaaatgca aatgcagccg caggagcagc aaacggcaag gcttatcgta gccagcctgc
    18361 ttagccactg ccgcgatttg ctcggcagcc aaatccagac cgcctcacgc agccaggcac
    18421 tatttgaagc tattcgcgat tatatcgacg aacgctatgc ctccgcgctt acccgcgaat
    18481 ctgtcgcaca ggcattctat atttcaccaa actatctctc gcacctgttt caaaaaacgg
    18541 gggccattgg ttttaacgaa tacctgaatc acacgcgact ggagcacgct aagacgttat
    18601 tgaaaggcta tgacctgaag gtaaaagaag tggcgcacgc ctgcggcttt gtcgacagca
    18661 actacttctg ccgtctgttt cgtaaaaaca ccgaacgctc gccgtcggaa tatcgccggc
    18721 agtaccacag ccaactgaca gaaaaaccga ctactccaga ataagatggg tttgctgcgg
    18781 cgcagaaagc actttgcgca ccgcgctcat tacccgctgc ggctcacgca ggaatgcgta
    18841 gatgctgcat tgcacatagc ggcaatgttc aaatcgctcg gcaccggcaa gctcgatatc
    18901 cgtaatcaac agagcaacat ccgcccgacg gatatcctcg tccgctaaac gattctccgt
    18961 tcccagcgcg ccctgagttt caatgctgac gccccacttc tctaactggc acaacttttc
    19021 cagccgttcc gccgccatat aagtatgcgc cacgccgctt acgcaggcgg ttactgccac
    19081 caggtatgcc attttaacct cccacggtaa cctgaaatcc ggcccgttct gccatttcgc
    19141 gcatcgttgt cacatcggct gacgacggcg caggcacctc tttcatcgac catgttttcc
    19201 ccagcaggcg gtatttcggt tcgccgtact gatgaaacgg taacagatgg atctgcctga
    19261 ttttcagcgg gatcagtaca tccagcgcct gctgcatatt ctccctgctg agcgtgaaac
    19321 cagggatcaa cggtaaacgc gggatcacgt tgacaccctc actcaccagc aaacgcagat
    19381 tctccagcac gcgtggcagg ttcatcttca ccacatcccg cgcctgagtc gcgtccataa
    19441 tttttaaatc gaacagcact tcatcgcaca atttcgccag cggtaacagc ttgcttgctg
    19501 gcgcgtctcc ggcagtttca atggcgcatg acacacccca cagccgcagt cgctgtaaaa
    19561 aacgggtagc aaactccgcc tgcattaaca cttcgccgcc agaaagcgtg acgccgccgc
    19621 cggacgtgcg aaagaaaatg tcatctttca tcacttcccg ttccagagcg tcaaggctga
    19681 tatcgcgacc aatccgttca aacgccccgg aggggcattc atccgcatca cgcaaacatt
    19741 tcgcacagtg cagacatttc gcctctctgc gtaccgtctg gattttgccg gagatcgact
    19801 ccggattagc acaccacggg caaagatgcg gacagccttt aaaaaagacc accgtacgaa
    19861 tgccctcgcc gtcattcagt gaataacgct gaatgttgaa aatgcgcgcc acatcatcgc
    19921 ggcgcgtttc cacaacgtta cagctgatgc gctgtccggc ggatgatgtc atcctggatc
    19981 tccttcgaca gttcgacaaa gaaggcgctg tatccggcaa cgcgcaccac cagcccggca
    20041 taatcttgtg ggcgctgttg cgcttcccgc aacgtgtcgg cgttcaccac gttaaactga
    20101 atatgctgca acttaagctg ggtaaacgcc cgtaagaagt cggccagttt gcgtaatccc
    20161 gcttcacctt ccagggtcgc cggagtgaat ttcacgttca gcaacgtacc gttagacagc
    20221 agcgtattat cgagcttact gactgacttc agtaccgctg ttggcccttg tgcgtcctgg
    20281 cccagcatag gtgacaagcc gccgtctgcc agttgttccc cggcaaaacg accgtctggc
    20341 gtcgcgccaa ccaccgatcc caacgggacg tgagcagaaa cggtatacga tcccggcgtg
    20401 aagtaaccgc gcgcgggttc tggtattttt ccacttcttt gcagtagtgg cgtaacagtt
    20461 cggcgctgat gttatccacc tcgtcgatat cgttaccgta tttctcgaag cggttaatta
    20521 agcgagcacg gactttttcg ccttctggcg ttgcaaagtt ggcttttaat accgacagca
    20581 attcgtcaaa acttaaacgc tgttgctcaa aaaccatccc tttcaacgca tggagagaat
    20641 cgctcaggtt ggcgataccg atcccctgta cgccggagaa gttataacgc gcgccgccat
    20701 cggtaatatc gcgacctttt tccagacaat cgctgataaa agacgagagc agcggtacag
    20761 gtgcccagtc gcgatggccg atatcacaaa tattgctgcc ctcaaccatc agggtgatgt
    20821 agtggctgat cttggcgcgg atctgctcca gtaaaccttc ataagtcagc gcagcattgc
    20881 cttcattttc atgcaggcag atttccatca ctttcagcag attaaacatc gcgatgtcat
    20941 gcaagccgta ggttctgccg ggaatagata attccacaca gcccactacg gaatagtcgc
    21001 gcgcatcttc cagcgaaacg ccacggttga ggaacgctgg taccaccact tcatcgttaa
    21061 agatttgcgg aataccggtg ccgaggcgaa tggtttcggc ggttttcatc aggaacggcg
    21121 tgtcgatcaa ggcgttagtg cgcacgccga ggttcggttg cggtaattgc acgctttgat
    21181 aggcgtcaag gcaaaggaac gaaagcacgt tcaccgcgct acgtccgttc tcggttaacc
    21241 cgccgagcag tgcggtatag ccggtcggga aacctgcgaa ataacgcgcg ctgctggtgg
    21301 agcgcaacag cacgatgtcg ttgcatttca cccataaaga ttcgagcagt tctttcagga
    21361 acgccggatc ttcgccctgg gttaaagatg cctgatagaa cggcaacata tactggtcga
    21421 agcgccctaa agacagcgaa ctggcgttgg attcgtattg cagaatgatg ttcatgtacc
    21481 agaataactg gcacgcctgc cagaacgtct gcggcttatg ttcggcgtta tggcgagaga
    21541 tctccgcaat agtcagcagt tcttcgcgac gctgtgcatc tgtgcagttt gccgccatcg
    21601 tttccgccag ttcggcgtaa cgcaaaatgt gtttctgcga ggcttccagc agtaacaacg
    21661 cggcctgata aaagtgattc tccggctgtt gctgacaatg ttgctgcatc tgtgccacca
    21721 gctcacccag accgtgattc aacaggcgtg ggtaatcaat aataatgtgc ccctggcctt
    21781 tatccgtctg gttgatgctg aaaatctgcg tgttggtcgc ggcttttact tcatctgtca
    21841 tctgcccgtt gatgaaatct ttcatcgaac gtttttccca gtacgggaac aactcttcac
    21901 ggtagatacg tttatcttct tcgctgatag caaagcggtc ctgcggacgc gtcgggaatt
    21961 gatccagctc tttcagcagc cagtaagggt ccatttccgg cgacataatc ccggcgcgcg
    22021 gttttacggt gcggttaccg gcaatcagtt cttcatcacg aatcgaaatt tcaacatgtt
    22081 caaggatata cgctgttgct ttcgcccggc gcaatatcac cggttcgcct tcggtttgcc
    22141 gatggctggc ggtataaagc agcgcccgct ccagcgagat ttcacgggta ttggcaaaca
    22201 gtgcagtttt gaggcgagag atacgattcg tcatgaggaa ctcctgtggg aaagcgaacc
    22261 cgcccttgcg agcgggagaa gaacttaagc ggtttgcgcc aggtgagctt caattttgct
    22321 cataatggcg tcagcacgtt ttactgcatc gctgatgttg acgcgcacga tggtcttgcc
    22381 agcaaaacgc tcttcaaact tgatgccgat atctttggtc agaatcacca tatcagcgct
    22441 cgccacatct tccgcagtca gttcattttc cagaccaatt gacccctagg tttctacttt
    22501 cacttcccag cctttcgctt tcgcggcact ttccagcgcc tctgctgcca tataggtatg
    22561 ggcaacacct gaaggacatg cggttactgc aataattttc gtcatggctc gttcctcaat
    22621 taattaattt caaaatccaa atccaggtcg tcttcttttt catcagtcga agacccattt
    22681 ttacgcgcca gacttttcag cacgttcaca caaacagccg tcaccactgc tccgacagcc
    22741 actgctgcga tataacccag cttgccttca accaccggca gcacaatcag accaccccag
    22801 cctgcatagc actgcgcacc gaccagcgcc gcagttactg caccacaaac tgaaccgacc
    22861 atgatggaag gaatgacacg cagcggatcg gcagcggcga aaggaatcgc cccttcagta
    22921 acgccaacac agcccatcac cagtgccgct ttgccggttt cgcgctcttc tgcggagaaa
    22981 tttttacgac caatcagcgt cgccagcccc atccccagcg gcgggataca aatacccacg
    23041 gcagcgatgg ccaccacggt ataaacaccc tgagcaacgc aaatcagcat gaaggcatag
    23101 gccactttgt taaccggacc gcccatatcg aacgccagca tcagacccat gatcaccgcc
    23161 agcataacaa tgctgccctg ctgcatcccc tgaagccact gagtcaggct gttggtcaac
    23221 gcccctacag gctcgcccaa gccccacatc atgatgcctg cggtaatcag tgtgccgacg
    23281 ataggaataa tgaagatagg catcaccgag cgcagaactt tatgcaccgg aattttcttc
    23341 aggtaatgca ccacgatgcc gccgataatc ccggcgatca gtgcaccaaa gaagcccgca
    23401 ccaaagctgt taccaaccca ggtaccgata gcgcatggag ccagcgcaga acgttctgca
    23461 atggaataac caatgtaagc ggcgaggaaa ggcaccatca gcgtcaagcc cgcaacgccg
    23521 atatcaaaca gttttttcag atttggatcg gctacggcat ccggcactgc gcctttgcca
    23581 tacaacatga cggaaaccgc cagcaaaata ccgcccgata ccacgaaggg aatcatgtgt
    23641 gaaacgcccg tcattaaatg ctgacgggta tttttcagga tctgcaccaa ctcattcata
    23701 agctgctccc gggcttgcga aagcccatgt ccatattttt gatggaggaa acaataggca
    23761 atcgcaggcg gagcaaacag tggataaaaa ggccatttac tggacttttg taatatcagt
    23821 acaaaaatgc gatccgcctc ataacttgcg ataaaccgga agcatgccgc aaaacggcgt
    23881 cttgtgagac gcgtcgcaaa ttgacggtta cattacattt gtccagcttt tggcagattt
    23941 gtcacatggc aggccgttgc cgtgcgctct tatcctgtaa acagaaaacc gtaccgggag
    24001 agaggcaatg gccctgattg tggaatttat ttgtgagcta cctaacggcg tacatgcgcg
    24061 tccggcaagc cacgttgaaa cgctgtgtaa tactttttca tcacaaattg agtggcataa
    24121 cctgcacact gaccgcaagg gcaacgccaa aagcgccctt gcgctgattg gcaccgatac
    24181 gctggcgggc gataactgcc agttactgat ttccggggcc gacgaacagg aagcgcacca
    24241 gcgtttaagc caatggctgc gcgatgaatt cccccactgc gatgcgccgc tggcggaagt
    24301 taaatctgac gaactggaac cactgccggt ttcactgacc aatctgaatc cgcaaattat
    24361 ccgcgcccgc accgtgtgca gcggtagtgc aggcggcatt ctgacgccga tctcttcttt
    24421 agatctcaat gcgctgagta atcttcccgc agccaaagac gttgacgccg agcaatccac
    24481 actggaaaac ggcctgacgc tggtactgaa aaacattgag tttcgtctgc tggatagcga
    24541 cggtgctacc agcgcgatac tggaagctca ccgatccctg gctggcgata catctttacg
    24601 tcagcactta ctggcaggcg tcagcgaggg attaagctgc gccgaagcga ttgtcgccag
    24661 tgcgaatcac ttttgcgaag agttcgcccg ttccagcagc agctacctgc aagaacgtgc
    24721 cctggacgta cgcgacgtct gcttccagtt actccagcaa atctacggtg agcaacgctt
    24781 cccggcaccg ggcaaactga cgcagtccgc catttgtatg gctgatgaac tgacccccag
    24841 ccagttcctc gaactggata aaaatcacct caaaggtctg ttgctgaaaa gtggcggcac
    24901 cacctcacat acggtgatcc ttgcccgttc gttcaacatt ccgacgctgg ttggtgtgga
    24961 tattgatgcc ctcactccgt ggcagcatca aacgatttat atcgacggca acgccggggc
    25021 gattgtggtt gagccaggag aagccgtagc tcgttattat cagcaagaag cccgcgtaca
    25081 ggacgccctg cgtgagcaac agcgtgtctg gctgacccaa caagcccgta ccgctgacgg
    25141 tatccgcatt gaaattgccg ctaacatcgc tcactccgtg gaagcgcagg ccgcattcgg
    25201 caatggtgcg gaaggcgttg gtttgttccg cactgaaatg ctctatatgg atcgcaccag
    25261 tgcaccgggc gaaagcgagc tgtacaacat tttttgtcag gcgctggagt ctgccaacgg
    25321 acgcagcatt attgtgcgca ctatggacat tggcggcgac aaacccgttg attatctaaa
    25381 cattcccgca gaggcaaacc cgttcctcgg ttatcgcgcc gtgcgtattt atgaagaata
    25441 cgcgtcgtta tttaccacac aactacgctc gatcctgcgc gcctccgctc acggcagcct
    25501 gaaaatcatg atcccgatga tctcctcaat ggaagagatc ttatgggtga aagaaaaact
    25561 ggcagaagcc aaacagcaac tacgtaacga acacattccg tttgatgaga agatccagct
    25621 cggcattatg ctggaagtgc cgtcggtgat gttcatcatc gatcaatgct gcgaagagat
    25681 tgatttcttt agtattggta gtaatgacct gacgcaatat ctgctggcag tggatcgcga
    25741 taacgctaag gttactcgtc actacaacag cctgaatccg gcattcttgc gggcgctcga
    25801 ttacgccgtg caggcggtgc atcgccaggg taaatggatt ggtctgtgcg gtgagctggg
    25861 agcgaaaggt tccgtgctgc cgttgctggt cggcttaggg ctggatgaac tcagcatgag
    25921 cgcaccatca attccggcgg cgaaagcgcg gatggcgcaa cttgatagcc gtgagtgccg
    25981 caagttgctc aaccaggcaa tggcctgccg tacttcgctg gaagtggagc acctgctggc
    26041 gcaattccgc atgacccaac aagacgcacc gctggtcacc gccgagtgca tcacactgga
    26101 aagcgactgg cgcagcaaag aagaagtgct caaaggcatg accgataacc tgctgctggc
    26161 agggcgctgc cgctatccgc gtaaactgga agccgacttg tgggcgcgcg aggccgtttt
    26221 ctctaccggt ctgggcttta gttttgccat tccacacagc aaatcagaac acattgagca
    26281 atccaccatc agcgttgcgc gtctgcaagc gccggtgcgc tggggtgatg atgaagcgca
    26341 attcatcatc atgttaaccc tgaacaaaca cgctgcgggc gatcagcaca tgcgcatttt
    26401 ctcgcgcctc gctcgccgca tcatgcacga agaattccgt aacgcgctgg ttaacgccgc
    26461 ctctgccgac gctatcgcca gcctgctgca acatgaactg gaactgtaaa aggaaacatc
    26521 atggaactgt atctggacac cgctaacgtc gcagaagtcg aacgtctggc acgcatattc
    26581 cccattgccg gggtgacaac taacccgagc attatcgctg ccagcaagga gtccatatgg
    26641 gaagtgctgc cgcgtctgca aaaagcgatt ggtgatgagg gcattctgtt tgctcagacc
    26701 atgagccgcg acgcgcaggg gatggtggaa gaagcgaagc gcctgcgcga cgctattccg
    26761 ggtattgtgg tgaaaatccc ggtgacttcc gaaggtctgg cagcaattaa aatactgaaa
    26821 aaagagggta ttactacgct tggcaccgca gtgtacagtg ctgcacaagg gttactggcc
    26881 gcgctggcag gggcaaaata cgttgctccg tatgttaacc gcgtagatgc ccagggcgga
    26941 gacggcattc gtacggttca ggagctgcaa gcgctgttag aaatgcacgc gccagaaagc
    27001 atggtgctgg cagccagctt taaaacgccg cgtcaggcgc tggactgttt actggcagga
    27061 tgtgaatcca tcaccctgcc cttagatgta gcgcaacaaa tgctcaacac ccctgcggta
    27121 gagtcagcta tagagaagtt cgaacacgac tggaatgccg catttggcac tactcatctc
    27181 taaaggagca attatggacc gcattattca atcaccgggt aaatacatcc agggcgctga
    27241 tgtgattaat cgtctgggcg aatacctgaa gccgctggca gaacgctggt tagtggtggg
    27301 tgacaaattt gttttaggtt ttgctcaatc cactgtcgag aaaagcttta aagatgctgg
    27361 actggtagta gaaattgcgc cgtttggcgg tggatgttcg caaaatgaga tcgaccgtct
    27421 gcgtggcatc gcggagactg cgcagtgtgg cgcaattctc ggtatcggtg gcggaaaaac
    27481 cctcgataca gccaaagcac tggcacattt catgggtgtt ccggtagcga tcgcaccgac
    27541 tatcgcctct accgatgcac cgtgcagcgc attgtctgtt atctacaccg atgagggtga
    27601 gtttgaccgc tatctgctgt tgccaaataa cccgaatatg gtcattgtcg acaccaaaat
    27661 cgtcgctggc gcacctgcac gtctgttagc ggcgggtatc ggcgatgcgc tggcaacctg
    27721 gtttgaagcg cgtgcctgct ctcgtagcgg cgcgaccacc atggcgggcg gcaagtgcac
    27781 ccaggctgcg ctggcactgg ctgaactgtg ctacaacacc ctgctggaag aaggcgaaaa
    27841 agcgatgctt gctgccgaac agcatgtagt gactccggcg ctggagcgcg tgattgaagc
    27901 gaacacctat ttgagcggtg ttggttttga aagtggtggt ctggctgcgg cgcacgcagt
    27961 gcataacggc ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaaagtggc
    28021 atttggtacg ctgacgcagc tggttctgga aaacgcaccg gttgaggaaa tcgaaaccgt
    28081 agctgcactt agccatgcgg taggtttgcc aataactctc gctcaactgg atattaaaga
    28141 agatgtcccg gcgaaaatgc gaattgtggc tgaagcggca tgtgcagaat gtgaaaccat
    28201 ccacaacatg cctggcggcg cgacgccaga tcaggtttac gccgctctgc tggtagccga
    28261 ccagtacggt cagcgtttcc tgcaagagtg ggaataacct actccaaact cccggcttgt
    28321 ccgggagttt gaacgcaaaa ttgcctgatg cgctacgctt atcaggccta cgcaatctct
    28381 gcaatatatt gaattttctt gttttttagg acagataagg cgttcacgcc gcatccggca
    28441 ctctgtgcca gtaatctgaa actcccacgt tgttcaggag ttttttatcc gcttctggac
    28501 attttctcta cagaacaatc gttatcccgg aaggtcgaat ctgtgactct tattttcacg
    28561 gacgaaccag atgaaagctt cactggcgct gctcagcctg ctaacggcat ttaccagcca
    28621 ctccttaaaa tctcccgccg ttccaccaac cgtggtacaa atccaggcca ataccaacct
    28681 tgccatcgca gacggtgcca gacagcaaat cggcagcacg ctattttacg atccggcgta
    28741 tatgcagctt acctatcctg gcggtgatgt cccgcaaaaa cgcggggtat gttccgatgt
    28801 ggtgatccgt gcattgcgca gccagaaggt cgatttgcag aaactggtgc acgaagatat
    28861 ggcgaagaat tttgccgagt acccgcaaaa atggcagtta aagcgcccgg acagcaacat
    28921 cgatcaccgt cgggtgccta atctggaaac ctggtttacc caccacgata aaacacgccc
    28981 catcagcaag aaccccagtg actatcaagc gggcgatatt gtctcctggc gattagataa
    29041 cgggctggca catattgggg tggtttcaga tggcttcgcc cgcgacggta cgccgctggt
    29101 gatacataac attggcgcag gtgcgcagga agaagatgtg ctgtttagct ggcgcatggt
    29161 ggggcattat cgttattttg ttaaataagc gatcgcccca catgaagaaa aaattcagat
    29221 cctttttaca ctaacggctt cttttttctt acggctcacc agcgccagtg ccatcacaat
    29281 cagcacaata ccgctacctt caacggcccc cggattctcg ccgagcagcc accaggaaaa
    29341 gagtacgccg caaacgggaa cggccagtgt gcttaagctg gcaatactgg caggcaagtt
    29401 tttcaataca aacaaccata agctccacgc cagtgccgtc gccagaatcg cactgtaggc
    29461 cagcgcccag aacacggtgg gctgccagtc aatttcacgt tgcggcacca gtaaagcgac
    29521 cacactcatc accagcgccg catagagcat ctgccaggat gttaacgaca ataaatccac
    29581 gcgcggatga cgggcataca gacgtttagc aacaatcgcg ctcgcccccc aactgacgcc
    29641 ggagaggatt gccagcatgg cacttttcat cgaagagaaa tcgagttgcc acggctgcaa
    29701 caccaaaaat aagccgaaag cggcaatcag tatcgcgaaa tattgcccac gtcgcaggcg
    29761 ttcaccgaga aacaacgcgg cgaaaatcac cacccagaac ggcatggtat agcttaggat
    29821 cgccaccttc cccgctccgc cgctgaccag cgcccactgc gccaggccaa ccatcccgca
    29881 ggtttgtaac agggcaatgg ctaaggtgta tttaaacggt gtcgggcgca ttccgcgacc
    29941 acgtaataaa aggacgatga ataaaacgag agcgccgaaa atgcagcgta aggcggtaaa
    30001 gtcgaaggca ccgatgtagc tggtgacttg cttcatgaaa atccagctat aactccagat
    30061 aagtgtgagc acaaccaggc cactgattgc cagtgggttg ctctttcctg cggcagacat
    30121 gagagatcca gtgtgtagtt ttcctgtgga cactatacgc cggtaaagaa acttcagcga
    30181 ctgcctgaaa ggcagtcgct gaagaaggtc agattacagc aggtcgaaac ggtcgaggtt
    30241 catcactttc acccatgccg ccacgaagtc tttaacaaac ttctcgtgag catcgctgct
    30301 ggcgtaaact tcagccaccg cacgcaggac agagttagaa ccaaatacca gatccgcacg
    30361 gctggcagtg tatttcactt cgccggtttc gcggtcacgg ccttcaaaca gctctttcga
    30421 ttcgtcggtc gctttccact cgtaacgcat atccagcaag ttcacgaaga agtcattgct
    30481 caatacgcca acgcggtcag tgaagacgcc gtttttgctg ccatcgaagt tggcacccag
    30541 tacgcgcatc ccgcccacca gcgcggtcat ttccggcgcg gtcagggtca gttgctgggc
    30601 tttgtcgatc agcagtgact cggtggtgga aacatccaga cgagcgcgat agttacggaa
    30661 accgtcagca attggctcca gcagctcgaa catctcaatg tcagtctgat cctgacgcgc
    30721 atcaacgcgg cccggcgcaa acggtacatg aatgctcaaa cctgcggcgc ttgcggcttt
    30781 ctcaacacca accacaccag ccagcacgat gatatccgcc agcgaggctt taccagactc
    30841 tttctggatt ttctccagaa caggcagagc acgaacggct gcggcgttca catcccagtc
    30901 gcgctgcggc attaatgcca gacgcgcacc gttggcacca ccgcgtttgt cgccaccacg
    30961 gaaggtagaa gcagatgccc aggccaccga tacccgctca ctaacagaca gaccagaatc
    31021 cgcaatcgcg aatttcagat cgataatgtc ctgctcggtc gggttgtaga tcggctgcgg
    31081 cagcggatct tgccagatca gatcttcttt cggcacttcc ggcccgatgt agcgagattt
    31141 cggccccata tccctgtgcg tcagtttgaa ccaggcacgg gcaaaggctt cgttgaacgc
    31201 ctgcggatcg ttgaggaaac gacgagagat cttctcgaac tcaggatcaa aacgcagcgt
    31261 caggtcggtc accagcatcg tcggtttacg tttcttcgac ggatcgaatg gatccgggat
    31321 aatttccggt gcgtctaccg cttcgaactg gattgcgcca gccggactgc gggtctgtac
    31381 ccactcatat ttgaacaggt tttcgaagaa atagttgctc cactgggtcg gcgtctgggt
    31441 ccagacaact tccagaccag aggtaatggc atctgcgcca acgccgctgc cgtaagtgct
    31501 cgcccagcct aaaccttgtt cttcaatcgg tgcagcttct ggatcaggac ctacgtttga
    31561 tgtcggaccg gcaccgtggg ttttacccag cgtatgacca cccgcaatca gcgccacggt
    31621 ttcttcgtcg ttcatgccca tgttgccgaa ggtcgcgcgg atagctgctg ccgcagaaag
    31681 cggttcgccg ctgtgatccg ggccttccgg gttaacgtaa atcaggccca tctcggttgc
    31741 acccagcggt gctttcgcca gcgcttccgg atgacggtga gtcagccagg ctttttcatc
    31801 accccagtta acatccagat ccggttccca gacgtcttca cgaccggcac caaaaccgaa
    31861 ggtacggaag ccggagtttt ccagcgccac gttacctgcg aggataaaca ggtcggccca
    31921 ggagattttc tgaccatatt tctgtttgat tggccacaac aggcgacgcg ctttatcgag
    31981 gcttacgtta tccggccagg agttcagcgg tgcaaaacgt tgctgaccac gacccgcgcc
    32041 gccgcgtcca tcgattgaac ggtaagtacc tgcgccgtgc caggccatac gaataaacag
    32101 accggcgtag ctgccccagt cggctggcca ccacggctga gattctgtca gcagggcttt
    32161 cagatctttt ttcaggccgt agtaatctaa tttgctgaat tctttgcggt agtcaaagtc
    32221 ctcacccagc gggttagaac gattagaatg ttggtttaac aggtcaacac ggagttgatt
    32281 tggccaccag tcgcgagtgg ttgtgcccgc ccccgcgctc tggtcgtgac cgccctgatg
    32341 gaacgggcat ttgccagtgg ctgtcgtgtt atggatatcg tctgacgtgc tcatcaatgt
    32401 gctcccctct acagtgttac cgttacgata cacagcgtta gagagaagtt ataattgaat
    32461 taatccacgg atgcgatagt tgagatctta catattgttg gttaaagaga tgtaggtcaa
    32521 attgatcgta attaagcttt tttgattatg tccgaattcg gacaaatgct ttataaaaaa
    32581 gggttttagc ttcatatcct tcaagtaatg agggagaaaa caaggctgac aaccttattt
    32641 tcccgccctc atttcgaggc agcattttgt gctctgttta aaatttgtga tcactgtgtg
    32701 attttcacaa aagccacact atttataaac caggtcgaac acccagcgta tggcaaatcg
    32761 cgtaactcat ttcagcgcgg ttaagcgtat agaagtggaa atctttcact ccttcacggc
    32821 ttaaaatctt caccatatcc atggcaatat tcgcgcccac cagtttgcgg gtttcggcat
    32881 catcatccag gccgtcgaac atttgcgcca tccacgccgg aatacgcacg ttggtcatat
    32941 cggcaaattt cttcgcctgt ttaaagttag ataccggcaa aattcccgga ataatttcca
    33001 catcaatgcc cgccgataca cagcggtcac gaaaacgcag gtagctttcg acatcgaaga
    33061 agaactgagt aatcgcgcgg ttggctccgg catcgacttt gcgtttcaga ttaagcaaat
    33121 ccgcctgagc gctttttgct tccgggtgaa cttccggata cgccgccacg gagatatcga
    33181 aatctgccac ttcttttaat agcgtcacca ggtcagaagc atacatttct ggcttaccac
    33241 ttcccggcgg cagatcgcca cgcagcgcca cgatatgacg aataccgtta ttccagtagt
    33301 cgcgtgcaat ggtgcgcaac tcgtcgggcg tcgcatcaat gcaagtaaga tgcggtgccg
    33361 cttccagacc agtgcgatct ttaatgcctt taataatgct gtgcgtacgg tcgcgctcgc
    33421 cggagttcgc gccataggtc accgatacaa acttcggttt caggctgcta aggcgatcga
    33481 tggagttcca cagggtctgc tccatttcac tggtacgcgg cgggaaaaac tcgaacgaaa
    33541 cgttaatctg cccctggact tctgccaggc tctgattcag ggcatcccgc tggctggcgt
    33601 gaaaaaagct cataccttac ctcatcaatc gcttgtagtt atatgttgtg ttgtgcacat
    33661 ctatccgttt agacgtccag atgtaaagat gaaggaaaag ccgaagggcg tcaaccgaaa
    33721 aatgaccaat gatggtgagg aaaaatcagg gaagatgaaa aaacttcagg atggtaagaa
    33781 aaagaaaatc cggtatgcaa agcaaaccgg actgcaaata taaactaacc atgtacttat
    33841 tgcttcttgc cggattcgtc gtaaacgctt aatccggcct aatcctatga taattcaata
    33901 tatttcatta gaaaccataa acccgaaaac atgagtaccg ggcattatta aatttctgaa
    33961 attacaacaa ctgtgccagc cggttgatat ccgactgaat cgccccggcg gtgacgtcgc
    34021 gcccagcgcc aggcccacgg atcaccagcg gattatcgcg ataccagcgg ctttcgatgg
    34081 caaagacgtt atcgcacggc agcagtgatg ccagcggatg gtcttcacgc accgcttcca
    34141 cgcccacacg cgctttgcca ttggcatcga aacgcgccac gtagcgcagc accagcccca
    34201 tttcgcgggc cgcttccagc cgttgcacca tctgctcgtt aagttcatcg ccattttcaa
    34261 agaaatggtc gatgctgccg ccttcgcaat gggcaggcac cagcgactcc acgcgtacct
    34321 gatccggttc gatgttgtaa cctgcttcac gcgccagaat caccagcttg cgcatcacgt
    34381 ctttgccaga gagatcgtca cgcgggtcag gttcggttaa gccctgctgc cacgcctgat
    34441 ccaccagctc ggtaaacggc acgctaccgt cgaattgcag gaacaaccag gagagcgtgc
    34501 cggagaagat cccgctgatc gacaaaatag tatcgccgct gtcgatcaga tcgcgcacgg
    34561 tgtggttgat cggtaagccc gcaccgacgg tggcattgta cagccagtga cgcccggttt
    34621 tttcgaaggc gtcgtggatc tggcgatatt tattgctgtc gctggctccc gccagtttgt
    34681 tggcgctgat gacgtggaaa ccgtggctgg cgaaatcaag atactgatca gcaagctgct
    34741 ggctggcggt aacgtccagc accactaaat catcatacgg atgggcgcgc atccacagga
    34801 acaacgactc ttcatcctgc tcaaccgctt catcgttgaa gaaggctaac gcgcggctgg
    34861 cgtccagccc gtcatagctc aacagactgc ggcggctgtc caccacgcct gccagcacaa
    34921 attcaaagtc ggtacgtgcc gaaagcgtgc tctgctcacg ggcgaacagt tccagccaac
    34981 gggaaccgat attgccctta ccgaacaata ccaggccgat gcgtttttct gcgcggaaga
    35041 cggactgatg cagcccctga atcaggcttt cggtcgggcc ggtgcgcagt actgccacca
    35101 ggctgatgcc gtcatcggac tgccaggtaa attcgaccgg ctggcctttc agttgctgcc
    35161 agaagcggtg gcaatgcagc gggttacggg tgacgcctgc accgaccatc gccaccagcg
    35221 ccagcccctg acgcaggcgc agttcgccag gtaatcccgc ttcgtcgagg attttcagcg
    35281 cactgtcggc cacttctgag gtgtagcaaa attgcagcaa ctggcgatcg ttatgtacgc
    35341 caaccgccag cgggcgtacc tgcgcgcgtt tcaggatttg gtcgatctct ttatgcgcca
    35401 gtttgaaatc ctgactggcg ggcacctgaa actcaatcaa acagacatca tcgtggctgg
    35461 tgacaatacg cgcaccagta ccggaggcca gcacgcgttc aatgcgcgtg gaaccttgat
    35521 ccggcgtgta gctacagcgc agttgcaggt cgatttcgct gccagaaacc ggctgtaaag
    35581 tacgggcgtg aagaacggga gccgccagac gagccagttc gctggcttca tccagacgca
    35641 gcaacggcag cagacaggca tctttcactt tacgcgggtc ggcactgtat accccggcga
    35701 cgtcgctcca gatggttaca cgagaaacac ccgccagcgc accgatttgt gtcgcggaat
    35761 agtcggaacc gttacgcccc agcagcaccg tttcaccggc gttgttacgg ctgataaacc
    35821 cggtcaccac cagacgtttg cccggatgtt gcaccagtag ctgttgcagc aacgggtaag
    35881 aaagcccttc atcaacctgc ggttgtgcgg cgcgttcagc gcgtaaaaac tcgcgggcat
    35941 caagccaggc cgctggcagc ccttgttgat taagtaccgc agacatcagg cgtgccgacc
    36001 atacttcccc gtggcccacc acttccgcat acactgcgtc gttaataccg ctgtcgagca
    36061 gcgccgccag gcgttccaga tcgctgacaa aagcgctaat gaggctatcg gcttcttcag
    36121 cgggtagcag accgctaatc agatcgcact gatagcgacg cagcgtttgt tgaacctgat
    36181 gcgcagagag acgatcggtc tggcttagtt tcaaccagtt aatcaactgg ttagtggtgc
    36241 taccggcggc ggaaaccacc atcatatcgt caggctgaga gtactccgcc ataatgcccg
    36301 cgacacgcaa ataacacttc acatcagcca gactactgcc accaaattta tgcagctgac
    36361 gacctttcgc ccctgcctgc gcaatcacac tcatttttac cccttgtttg cagcccggaa
    36421 gccattttcc aggtcggcaa ttaaatcttc gccatcttca ataccggtgg agatacgcag
    36481 cagcgtctcg gagatcccga cggcagcacg cgcttctggt gccatgcctg catgtgtcat
    36541 ggttgcggcg tgagagatta aactttccac tccccctaat gattccgcca gcgtaaacaa
    36601 cgacagcccg ctcaggaaac gacgcagcgt ctgctcatcg ccatccagtt caaaactcaa
    36661 cattgcgcca aagccttttt gctgacgcgc ggcaatttca tgcccctgat tttccggcag
    36721 cgacgggtga tacagttttt tcaccaacgg ctgggtttgc agatatttca caatcgcctg
    36781 cgcgttgcgc tgcgccagct ccatacgcgg caccagcgtt cgcaacccgc gtagcagcag
    36841 atagctgtca aacgcgccgc ccgtcacgcc aatattgttt gcccaccagg caagttcagt
    36901 gacaacgtcc gggtctttag caatcaccac gccagccacc acgtcagagt gaccgttcag
    36961 atatttcgtg catgaatgca acaccagatc ggcacctaat gccagcggat tttgtaatgc
    37021 cgggcttaag aaggtgttat ccaccacgct caccgccccg acttcccttg ccagatggca
    37081 gattttcgca atatccacga cgcgtaacaa tggattactt gggctttcta ccagtaccag
    37141 tttgggtttt tctgccagcg ctgcccgtaa tgcctgttca tcgccttgat caacaaacaa
    37201 cacgcgatag caaccgcgtt tcgccagact gtcgaacagg cgatagctac cgccgtagca
    37261 gtcgtgcggc gcaaccagca gatcgccagg tttcaaaaag acggtcgtta ccaggtgaat
    37321 cgcggacatg ccggtattag taagtactgc gccagcaccg ccttccagtt ctgccagcgc
    37381 acgctgaacc acatcgcgcg ttgggttgcc gcgacgcgag taatcatgcg cgcgcggttc
    37441 attaaatccg gtaaagttat aggtgctgga aagatggatg ggagggacaa cgcaaccata
    37501 ctgttcgtcg tcatttaacc cgctacgcac tgcgatggtg gcctgtttac gcgtcatgtg
    37561 atgaagtttc ctgggctttg tcggtgaaat gtcaggcacc agagtaaaca ttgtgttaat
    37621 ggacgtcaat acatctggac atctaaactt ctttgcgtat agattgagca aatcccaaat
    37681 agccgttaaa attatatgca ttatcacgcc gacaggtgca ttacacgatg tcacggtaac
    37741 gcctgtacgg taaactatgc gggtttacgg tcagtaccca cataaattgt gtggtctggt
    37801 ctcaatttat tgacgaagag gattaagtat ctcatggctg aatggagcgg cgaatatatc
    37861 agcccatacg ctgagcacgg caagaagagt gaacaagtca aaaagattac ggtttccatt
    37921 cctcttaagg tgttaaaaat cctcaccgat gaacgcacgc gtcgtcaggt gaacaacctg
    37981 cgtcacgcta ccaacagcga gctgctgtgc gaagcgtttc tgcatgcctt taccgggcaa
    38041 cctttgccgg atgatgccga tctgcgtaaa gagcgcagcg acgaaatccc ggaagcggca
    38101 aaagagatca tgcgtgagat ggggattaac ccggagacgt gggaatacta aagcaaaaaa
    38161 gagcggcgcg gagtggaatc gcctgatgcg ctacgcttat caggcctacg tcatattgca
    38221 atttattgaa tttgcacgaa cttgtaggcc ggataaggcg ttcacgccgc atccggcata
    38281 aacaacgagc acgttgtctg cgacccaccg ctttttatac atggacgttt aactatgaaa
    38341 aacaggctgc tgatcctcag cctgctggtt tctgtacctg cctttgcctg gcagccacaa
    38401 accggcgaca tcatctttca gatctctcgc tcatcgcaaa gtaaagcgat ccaactggcg
    38461 acccattccg attatagcca caccggtatg ctggtgatac gcaacaaaaa gccctacgtt
    38521 tttgaagcgg tcggcccggt gaaatacacc ccgctcaagc agtggatcgc ccatggtgaa
    38581 aagggcaaat acgttgttcg ccgcgttgaa ggcggactga gtgttgaaca acagcaaaaa
    38641 ctggcgcaaa cggcaaaacg ttatctcggt aaaccgtatg atttcagctt ctcatggagc
    38701 gacgatcgcc agtactgttc ggaagtggtg tggaaggttt accagaacgc gctgggaatg
    38761 cgcgtgggcg agcagcagaa gttgaaagag ttcgatctca gcaacccgct ggtccaggct
    38821 aagctcaaag aacgctacgg caaaaatatt ccgcttgagg aaacggtagt ttcaccacaa
    38881 gccgttttcg acgcgccaca actcaccaca gtcgccaaag agtggccgct gttttcgtgg
    38941 taatggatga tgaaaaaaat aattcccgcc gtagtctgtt tgttgctttg tagttgcacg
    39001 acctttccgc aagctgtcgc tccggtaaac ggtgatgcct ccaattagtt gaattaagga
    39061 tataatactc gcttttaagg ttgtttcatg gtcacagtta accttcattg cccccgttgc
    39121 cagtccgttc aggtttaccg tcacgggcag aacccaaaaa gacatgagcg attccgcggc
    39181 cgtgactgtc accgggtatt tcagctcagt tactgttacg agccccgtaa accgggagtt
    39241 aaagaccaaa tcgctgaaat gactttcaac ggtgccgggg ttcgcgatac tgcccgtacg
    39301 ctgaaaatcg gcattaatac agtcatccga actttaaaaa actctcgcca cgaagaataa
    39361 cgtcatcccc ggtcgctcat gctgatgtgg cactcatctg caaacttagt cagcaatgga
    39421 gctttgtcgg cagtaaagcg cgacagcact ggctctggta cgtgtacaac acgaaaactg
    39481 gtggtgttct ggcctacact tttggccccc ggactgacga aacctgtcgt gaattgcggg
    39541 cgctgcttac ccttttacca tcggcatgtt gaccactgac gaatggggta gttacgctag
    39601 agagttaccg aaggagaagc atctgactgg taagattttt actcagcaca ttgagcgtaa
    39661 caatctgacc ctgagaaccc gaatcaaacg tctggcccgc agaaccatct gtttctcgcg
    39721 ttctgtagaa cttcatgaaa aagtcatcgg tgcttttatc gaaaatatat gttctactaa
    39781 ttggagtcat cacccttctg ataagctttc cttggatgat taccagactg aatacacagc
    39841 caaagatttt tacctcattc tgctcttttt tctcaaatga catatattat attttacatc
    39901 atattcatag gcatatgaca ttcccccgcc ccccattatg tcattgtggg ttgatgttgg
    39961 gctttcataa aaaaaacatc tttttttgaa ctagcattaa ttatatttac tttatatatt
    40021 aaaatatttt ctccatctgg aaatatttta gccacacaat ataaagcagc tgaatccgaa
    40081 ttacttaaaa atatattatc atcacccata actccagcca ttaaatgact ggagactaaa
    40141 gcaaggaaat aacaacattg tattgccttc attttaatta ctcccttgcc tgataattgt
    40201 aactccattc cctttagctt tgggattata ttttagatag gtgttatttg gtgttcccaa
    40261 gtaactactg tattcttttt ttcccattcc attcatataa gaattcgtta aatctttgct
    40321 tgaaaaattt agactatcat aaacatcatt ttcttttgtt actttatttc ctttatcgtc
    40381 agagtaaaaa ccgtgagtgt gataatcgcc tactctctct gaaccttcag ggcattttat
    40441 atttcgcaag tctacactat cattatcatt tgaacttatc ggattcatgg ggaaatattc
    40501 accttgcttc ttacatatta gtccaccata ttctttattc tcgttaattg attctccatt
    40561 acacattgcc aatgcaaata ttccaatatc atcaatattt tttgatttaa attcatataa
    40621 tcctaatgga tccatgctat tcaccgggct cagcggatat gtatacaggt tccatccccc
    40681 cttcagccca atcggatcct gagtgatata ccgcccctgc agcgggtcat aatagcggtg
    40741 gcggttataa tacaggccgg actcctcatc atactgctgc cccggcaaac gaataagctg
    40801 ctgcagctga tgcgggttct cttcattcag caggttgccc cattcatcat attcccggcg
    40861 tatttccagc ccgtcaggcc cggtggctga cgttaactgg ctgtggtggt tatagtaaaa
    40921 cgccgatgcc ctgccatccg gcgtggtgat gcgcgtgatg agacccgtca ccacatccgg
    40981 gctgtattct gttgtccggc ctg
//
LOCUS       DAQSWQ010000028        40073 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.028, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000028 DAQSWQ010000000
VERSION     DAQSWQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.028"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(48..123)
                     /locus_tag="R8G18_001526"
     tRNA            complement(48..123)
                     /locus_tag="R8G18_001526"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:Lys,seq:ttt)
     gene            complement(157..232)
                     /locus_tag="R8G18_001527"
     tRNA            complement(157..232)
                     /locus_tag="R8G18_001527"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Lys,seq:ttt)
     gene            complement(379..454)
                     /locus_tag="R8G18_001528"
     tRNA            complement(379..454)
                     /locus_tag="R8G18_001528"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(419..421),aa:Lys,seq:ttt)
     gene            complement(458..533)
                     /locus_tag="R8G18_001529"
     tRNA            complement(458..533)
                     /locus_tag="R8G18_001529"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(498..500),aa:Val,seq:tac)
     gene            complement(683..758)
                     /locus_tag="R8G18_001530"
     tRNA            complement(683..758)
                     /locus_tag="R8G18_001530"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(723..725),aa:Lys,seq:ttt)
     gene            complement(923..1714)
                     /gene="cpoB"
                     /locus_tag="R8G18_001531"
     CDS             complement(923..1714)
                     /gene="cpoB"
                     /locus_tag="R8G18_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HEE9795258.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1724..2245)
                     /gene="pal"
                     /locus_tag="R8G18_001532"
     CDS             complement(1724..2245)
                     /gene="pal"
                     /locus_tag="R8G18_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEE9795259.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2280..3572)
                     /gene="tolB"
                     /locus_tag="R8G18_001533"
     CDS             complement(2280..3572)
                     /gene="tolB"
                     /locus_tag="R8G18_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEE9795260.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3705..4937)
                     /gene="tolA"
                     /locus_tag="R8G18_001534"
     CDS             complement(3705..4937)
                     /gene="tolA"
                     /locus_tag="R8G18_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEE9795261.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(5002..5430)
                     /gene="tolR"
                     /locus_tag="R8G18_001535"
     CDS             complement(5002..5430)
                     /gene="tolR"
                     /locus_tag="R8G18_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HEE9795262.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5434..6126)
                     /gene="tolQ"
                     /locus_tag="R8G18_001536"
     CDS             complement(5434..6126)
                     /gene="tolQ"
                     /locus_tag="R8G18_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HEE9795263.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6123..6527)
                     /gene="ybgC"
                     /locus_tag="R8G18_001537"
     CDS             complement(6123..6527)
                     /gene="ybgC"
                     /locus_tag="R8G18_001537"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEE9795264.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6677..6970)
                     /gene="ybgE"
                     /locus_tag="R8G18_001538"
     CDS             complement(6677..6970)
                     /gene="ybgE"
                     /locus_tag="R8G18_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HEE9795265.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6970..7083)
                     /gene="cydX"
                     /locus_tag="R8G18_001539"
     CDS             complement(6970..7083)
                     /gene="cydX"
                     /locus_tag="R8G18_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEE9795266.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7098..8237)
                     /gene="cydB"
                     /locus_tag="R8G18_001540"
     CDS             complement(7098..8237)
                     /gene="cydB"
                     /locus_tag="R8G18_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEE9795267.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8253..9821)
                     /gene="cydA"
                     /locus_tag="R8G18_001541"
     CDS             complement(8253..9821)
                     /gene="cydA"
                     /locus_tag="R8G18_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEE9795268.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10669..13302)
                     /gene="mngB"
                     /locus_tag="R8G18_001542"
     CDS             complement(10669..13302)
                     /gene="mngB"
                     /locus_tag="R8G18_001542"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HEE9795269.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(13320..15236)
                     /gene="mngA"
                     /locus_tag="R8G18_001543"
     CDS             complement(13320..15236)
                     /gene="mngA"
                     /locus_tag="R8G18_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HEE9795270.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15405..16127
                     /gene="mngR"
                     /locus_tag="R8G18_001544"
     CDS             15405..16127
                     /gene="mngR"
                     /locus_tag="R8G18_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HEE9795271.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(16231..17100)
                     /gene="sucD"
                     /locus_tag="R8G18_001545"
     CDS             complement(16231..17100)
                     /gene="sucD"
                     /locus_tag="R8G18_001545"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEE9795272.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(17100..18266)
                     /gene="sucC"
                     /locus_tag="R8G18_001546"
     CDS             complement(17100..18266)
                     /gene="sucC"
                     /locus_tag="R8G18_001546"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEE9795273.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18360..19577)
                     /gene="odhB"
                     /locus_tag="R8G18_001547"
     CDS             complement(18360..19577)
                     /gene="odhB"
                     /locus_tag="R8G18_001547"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEE9795274.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19592..22393)
                     /gene="sucA"
                     /locus_tag="R8G18_001548"
     CDS             complement(19592..22393)
                     /gene="sucA"
                     /locus_tag="R8G18_001548"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEE9795275.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22694..23410)
                     /gene="sdhB"
                     /locus_tag="R8G18_001549"
     CDS             complement(22694..23410)
                     /gene="sdhB"
                     /locus_tag="R8G18_001549"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HEE9795276.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23426..25192)
                     /gene="sdhA"
                     /locus_tag="R8G18_001550"
     CDS             complement(23426..25192)
                     /gene="sdhA"
                     /locus_tag="R8G18_001550"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEE9795277.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(25192..25539)
                     /gene="sdhD"
                     /locus_tag="R8G18_001551"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(25192..25539)
                     /gene="sdhD"
                     /locus_tag="R8G18_001551"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HEE9795278.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25533..25937)
                     /gene="sdhC"
                     /locus_tag="R8G18_001552"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25533..25937)
                     /gene="sdhC"
                     /locus_tag="R8G18_001552"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HEE9795279.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26318..26425
                     /locus_tag="R8G18_001553"
     CDS             26318..26425
                     /locus_tag="R8G18_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795280.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26631..27914
                     /gene="gltA"
                     /locus_tag="R8G18_001554"
     CDS             26631..27914
                     /gene="gltA"
                     /locus_tag="R8G18_001554"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEE9795281.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28304..28870
                     /locus_tag="R8G18_001555"
     CDS             28304..28870
                     /locus_tag="R8G18_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEE9795282.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28921..31379
                     /locus_tag="R8G18_001556"
                     /pseudo
     CDS             28921..31379
                     /locus_tag="R8G18_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            31394..32125
                     /locus_tag="R8G18_001557"
     CDS             31394..32125
                     /locus_tag="R8G18_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEE9795283.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            32122..33183
                     /locus_tag="R8G18_001558"
     CDS             32122..33183
                     /locus_tag="R8G18_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9795284.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            33335..34381
                     /locus_tag="R8G18_001559"
     CDS             33335..34381
                     /locus_tag="R8G18_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEE9795285.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34378..35169)
                     /gene="nei"
                     /locus_tag="R8G18_001560"
     CDS             complement(34378..35169)
                     /gene="nei"
                     /locus_tag="R8G18_001560"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HEE9795286.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(35205..35939)
                     /gene="pxpA"
                     /locus_tag="R8G18_001561"
     CDS             complement(35205..35939)
                     /gene="pxpA"
                     /locus_tag="R8G18_001561"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEE9795287.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35929..36861)
                     /gene="pxpC"
                     /locus_tag="R8G18_001562"
     CDS             complement(35929..36861)
                     /gene="pxpC"
                     /locus_tag="R8G18_001562"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HEE9795288.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36855..37511)
                     /gene="pxpB"
                     /locus_tag="R8G18_001563"
     CDS             complement(36855..37511)
                     /gene="pxpB"
                     /locus_tag="R8G18_001563"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEE9795289.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37534..38277)
                     /gene="ybgI"
                     /locus_tag="R8G18_001564"
     CDS             complement(37534..38277)
                     /gene="ybgI"
                     /locus_tag="R8G18_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HEE9795290.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38548..40029
                     /gene="dtpD"
                     /locus_tag="R8G18_001565"
     CDS             38548..40029
                     /gene="dtpD"
                     /locus_tag="R8G18_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HEE9795291.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9695 a        10771 c         9763 g         9844 t
ORIGIN      
        1 gaatatctca gatagcaaaa aagcgccttt agggcgcttt tttacattgg tgggtcgtgc
       61 aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga
      121 ccccttgcgg gattatgctg ctttaatcat tcaggatggt gggtcgtgca ggattcgaac
      181 ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccatacgggt
      241 gttacctgaa agatttctac tcggggccaa tctttaagat tggtgggtcg tgcaggatga
      301 ctcgcttcgc ttgccctacg ggccgttgct ggcgcaacgt tatccttcac gcttaacatc
      361 tgagtttgat gttaaaattg gtgggtcgtg caggattcga acctgcgacc aattgattaa
      421 aagtcaactg ctctaccaac tgagctaacg acccgagtgg tgggtgatga cgggatcgaa
      481 ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca cccgatactg
      541 cgctggatac tacaaactac ttccaccgaa cccaccgtaa ttagtggtgg gtcgtgcagg
      601 atgactcgct tcgctcgccc tacgggccgt tgctgacgca acgttatcct acacgcttaa
      661 catctgtctt cgatgctaaa actggtgggt cgtgcaggat tcgaacctgc gaccaattga
      721 ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg ctttcggttt
      781 gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga gcgcaacaaa
      841 aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag aaataatgcg
      901 acttctggtc gtgtgttatg cattacatcg cgttcagacg tttttgtgcc tgtttagcgc
      961 catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct tttgcggtgt
     1021 cacctttgtc ctgcatgatg acgccgactt taaacatcgc atcagcagcc tttggtgact
     1081 tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt ttacccttgt
     1141 tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa tcagggtaat
     1201 ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta tcctgcacca
     1261 gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc gcgccagcat
     1321 tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt tgatcgctgc
     1381 tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac aggatctgct
     1441 tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga ccacgcaggg
     1501 aatcaatatc ggattgatta tcagaaagtt gttgctggag ttgggttaaa agctggctgt
     1561 gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct gagccgacac
     1621 tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc agtaacgaca
     1681 gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa accagtaccg
     1741 cacgacggtt tttggagtat gccgcttcgt catgacccag tactgcaggt ttttctttac
     1801 cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg tacatcttaa
     1861 cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca cgttcgtccg
     1921 cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt gcatgtgcat
     1981 ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag taaacgatgt
     2041 tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac atgttgccgt
     2101 tgccgccgtt cgcatccata ccagtgccgg cacccagcat gccttcgctg ccgtcattgc
     2161 tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca atcatcagcc
     2221 ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat taattattat
     2281 cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg aagacgcgct
     2341 ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg agaagagctg
     2401 tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa cgtggacgac
     2461 agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg cccaccgttg
     2521 gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa
     2581 ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata aacctgtgga
     2641 cgaccggcct ggtcagaagt aaatgccagg ttctggctgt ccgggaacca ggtcggttcg
     2701 gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa atccattacg
     2761 tacaggttca ggctaccggt tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag
     2821 aatgcaggcg caccgttgtg acgcgggaat gaagccacct gacgtacagc gccgtttgcc
     2881 agcgtttgga taactagcgc ggaacgaccg ctttcgaagg tcacataagc cagtttagaa
     2941 ccgtctggtg accacgccgg ggacatcagc ggctgtggtg aacggtgaac gacaaactgg
     3001 ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc gttggtctga
     3061 acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt ttcaaacact
     3121 tcatcactgg cggtatgacc agcatagcgc agccactgct tgttcacttt gtacgagttc
     3181 tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa
     3241 gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatacc cagcgcggac
     3301 catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg agcgcgatct
     3361 aacggattaa atttaccgct gttacgcaag tcggcagcaa cgatgccgcc aatatcttca
     3421 ggtgccgcac cagggcccgc ccactggaaa ggaacaacac caataggacg accggaatct
     3481 acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca cagtatgaga
     3541 aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg accgatggcc
     3601 cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag aaccccgtgg
     3661 caaccggtgc ctgatgttga ccgtcggaac agtcaacatc gcgattacgg tttgaagtcc
     3721 aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg gatcttcgca
     3781 agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc aggtttgata
     3841 tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt gcctgcatag
     3901 gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc atagttattg
     3961 atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg cgaagcattg
     4021 ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag ctcaccgaaa
     4081 atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc tgcttttgcc
     4141 gctgccgctt ttttgtctgc ggcggctttt tctgccgctg cttttgccgc tgccgctttt
     4201 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt atcagcagcc
     4261 gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt ctttcttgct
     4321 tcagctgcag cagcttctgc cgcttccgct ttcttcttca gtgccgcagc ggctgcctcg
     4381 gcttttttct gcgcttcggc tgcggctttg gcggcttctg cttctgcttt tttcttcgcg
     4441 tctgccgccg ctttcttcgc tgcttcttcc gcagctttag catccgcttc ggccttcgct
     4501 ttagcatctg ccgccgcttt cgctgccgcc tcttccgctt gcttctgctt taactcggcc
     4561 tgttttgcgg cttcttcagc ctgctttttc tgttcctgag cagctaaccg ctctttctca
     4621 agttgcttca ggcgttcctg ttcagccgct tgtttctcac gcagttcttc agcagcctgc
     4681 tgttccttta tcttgcgctg ctcatcagaa cgcttcgcgc ttgattcctg gctttgcatg
     4741 cgtttgtact gctcaactac cgcacctgaa tcaaccatga cagcgtcgat ggacgaacca
     4801 ccgccgcctc cggctgaagc ttctatattc tcatcgaacg aactccagag cagcgccgca
     4861 aataagatga catgcagcac tgctgaaata attatcgccc gcttgagctt gtcgttttgt
     4921 tcggttgcct ttgacactct cggtttccaa aaactgttcg cctgttaccc gctctctttc
     4981 aagcaaggga aacgcagatg tttagatagg ctgcgtcatt aaaccaaccg atttcacacc
     5041 cgcactatgt aacaagttca gtgctttaat tatttcatcg taaggcacat cttttgcgcc
     5101 accgatcaga aagaccgttt tcgggttggc cttgaaacgg ctggacactt ccgccaccac
     5161 ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc accacggtgt actgaccaat
     5221 accagacact tcaacaatca ctggcggatt atcgttactg ctcaccgcct gtgattcagt
     5281 agcgtctggc agatcgacct ccacgctctg ggtgatgatg ggcgctgtcg ccataaagat
     5341 cagcaacagc accagcagta cgtccagcaa cggtacaatg ttgatttcgg acttgagatc
     5401 gcgacgacct cgtccacgcg ctctggccat ggcttacccc ttgttgctct cgctaacggt
     5461 aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata aagttgtcgt aattcagttc
     5521 cagtttgttt acgcgctggt tgaggcggtt ataagccatt accgccggaa ttgcggcgaa
     5581 cagaccaatt gcagtcgcaa tcaacgcttc tgcgataccg ggcgcaacca tttgcagtgt
     5641 tgcttgtttt accgccccga gggcgataaa ggcgtgcatg atcccccaga ccgtaccaaa
     5701 cagaccaata tacgggctga tggagccaac cgtaccgagg aacggaatgt gcgtttccag
     5761 attttcaagt tcacggttca tggagatacg catagcacgc gacgcccctt ccactacggc
     5821 ttccggcgca tggctattgg cacgatgcag gcgcacaaac tctttgaacc cgctgtaaaa
     5881 tatttgttcc gaaccagtca gattatcccg tttcccctga ctctcttgat agaggcgaga
     5941 gagttcgatt ccagaccaga atttatcttc aaacgcttcg gcttcgcgcg ccgctgcgtt
     6001 aagaatacgg gtccgctgga taataatggc ccaggatgcg attgaaaaac caatcaaaat
     6061 caacatgata agtttaacca gaaggctagc cttcaggaac aaatcaagga tattcatgtc
     6121 agtcactgct taaactccgc gacaatagac ttgggaagcg cacgaggctt cattttgagt
     6181 gggtcaacgc aaacaaccag aacctctgct tcattcagca aagtattctc ggcgttgaca
     6241 atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg ttatttcagt ctgtatttcg
     6301 agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg tcattttacg taccacaaag
     6361 gcaacgcgtt cagccatcag cgcctgctga ctgaagtgat gatgacgcag catctctgtg
     6421 cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca ccacaccacc ggcatcggta
     6481 tcttcatagt agacgcgaac cggccatcga aacagcgttg tattcacttt acatcccggt
     6541 aatgcaacaa aagttagagc ttttaaacgc tgctactata cgcgcgcgat tgaggtttgg
     6601 gaatgagggc aagttaaggg agcgtaaaaa tttatgggcc tggagcggcc cataattttt
     6661 taaagagaat tctgatttaa aagaagaaga aaatcagccc gacaatgaga acaatatcgg
     6721 caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg cggacgaaag cccacgccgt
     6781 gaatcacacc agcacagacg gcccacatca gcaataaacc atgccagatt tccagttcac
     6841 tggtcttcgc ggcaaaacga gatgggtccc aaaacataca tcctgctaac agaagcgcca
     6901 tcacgaagga aagcgcccgc agggggcgct tgtccattac cgcatacaag gtcgcgataa
     6961 tcttactcat cagatgtctt cttgaccggc tttgcctgat tcgacgtgtt caagcgccag
     7021 cgcggtgatt accccaaacg aacaggcaag aagcgttccc agaatccatg cgaaatacca
     7081 catttttagc tccttactta gtacagagag tgggtgttac gttcaatatc ttctttggtg
     7141 atacgaccga acattttcca gtaacaccaa gcggtgtaga gcagaatgat cggtaccaga
     7201 accaccgcaa cccaggtcat gacgttaagc gtcagctggc tggaagttgc atcccacatt
     7261 gtcagacttg cgttcatcat ggtgctggac ggcatcacaa acgggaacat tgcgataccg
     7321 gctgtcagga tgatgcaggc cagcgtcagg gaggagaaca caaacgccca cgcggcttta
     7381 tccatacgtg cagtcaggat ggtcagcagc ggaagaacca cacccagtgc cggaatagcc
     7441 cacagaattg gcgtgttgtt gaagttaacc agccatgcgc cagcttcacg aaccacttct
     7501 ttattcagtg ggttagaggc tgcgtaatgg tccattgtcg atttcacgac ataaccatcg
     7561 ataccgtaca tcacccatac gccagccagt gcgaaacaga ccagtgtcac cagcgcagcc
     7621 acctgagccg ttgcacgggt acgcaggtgc agttcgccca cggtacgcat ttgcagatag
     7681 gttgcgccct gagtaatgat catccctacg ctcaccacgc ctgccagcag gccgaacggg
     7741 ttaagcaact ggaagaagtt accggtgtag tacagacgca gatattcatc aacgttgaac
     7801 ggtacgccct gcaacaggtt accgaacgct acaccaatta ccagcggcgg aacgaaacta
     7861 ccaatgaaga tgccccagtc ccacatgtta cgccagcggg tttcttcaat cttggagcgg
     7921 tagtcaaaac cgaccggacg gaagaacaaa gacgccagca cgaggatcat cgccacatag
     7981 aagccggaga acgcagcggc atagaccatc ggccaggcag caaagagtgc gccgcccgcg
     8041 gtgatcagcc aaacctggtt accgtcccag tgtggtgcaa tggagttaat cataattcga
     8101 cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc ccacccccat gtcgaaaccg
     8161 tcagtgactg caaaaccaat cagcagaacg ccaaccagca gccaccagat aaaacgcaat
     8221 acttcataat cgatcatttg acgactcctg tcttagcgtg ccggctgagt agtcgtggaa
     8281 gactgctcaa agtgatagcg accggttttc aggctgcttg ggccgaggcg tgcaaacttg
     8341 aacattaaga acaattctgc caccaggaac agggtataca ggccgcaaat cagcaccatt
     8401 gagaagatga gatcgcctgc ggtcagtgac gagttcgcca cagctgtcgg cagcacttca
     8461 ccgatagccc acggttggcg gccatattca gccacgaacc agcccgcttc tacagcaatc
     8521 cacggcagcg gaataccgta cagcgcggcg cgcagaagcc attttttctc gccaatgcgg
     8581 ttgcggatga cactccagaa agagagcgcg atgattgcca gaagcaggaa gccacacgcc
     8641 accatgatac ggaacgcaaa gtacagcggc gctacacgcg ggatggagtc tttggttgcc
     8701 tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt cagcagcaga
     8761 ccgtaaccga ggtctttctt catgctattg aactggtcac gaaccgcctg gtcggtagaa
     8821 ccagaacgca gttgttcgag cagagagtac gccttcatcc cgttacgaat gcgttcttca
     8881 tgctgcacca tcagctcttt caggccgata accggggtat ccacggaacg cgttgcaatg
     8941 atgcccagtg cgtaagggat ctgaatcgca aatttgttcg tctcctcttc ctgatcagga
     9001 atgccgaaca gagtaaaggc agcaggcgca ggttgcgttt cccactcggc ttcaatagca
     9061 gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg attcatcacc cagaacaata
     9121 acagacagaa cagcagccat accgaagctg gcagcgatag caaaggagcg tttagcgaag
     9181 gcgaagtcac gacctttcag catataccat gcgctgatac cgaggatgaa catcgcgcca
     9241 gtcacataac cagacgctac agtgtgaacg aatttcacct gagcaaccgg gttaagcacc
     9301 agctcggaga agctcaccat ctccatacgc atagtttcaa agttgaaatc ggacgcgatt
     9361 gggttttgca tccagccgtt cgcaaccaga atccacagtg cggacaggtt tgaaccgagc
     9421 gccaccagcc aggtgacaca catatgctga actttaccca gacgatccca accgaagaag
     9481 aacagaccta caaaggtgga ttcgaggaag aaggccatca gaccttcgat tgccagcggc
     9541 gcaccgaaga tatcccctac atagtgggaa tagtaagacc agttagtccc gaactggaac
     9601 tccatggtca gaccggtagc cacacccaga gcgaagttga taccaaacaa cttgccccag
     9661 aacttggtca tatctttata aatctgtttg ccggagagga cgtagaccgt ttccataatg
     9721 gccagcagga acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc
     9781 aaggcaaact gtaagcgcga cagttcgact atatctaaca tcatgactcc ttgctcatcg
     9841 catgaagact ccgagagtga accccgttag aaagggtcac acgcatgccc caatagaaat
     9901 ttgtttgaac actttcatca taccatgtga atgcacacaa aaacggcgta aagaaaggtt
     9961 gcaaacatgt taataaaaac tcaaattgat cccacgtata tattacgccg caaaatcctt
    10021 acaataaaca ggtttttgtt gaagcaaatt tgcatttttc gacggtgatc aatttatagc
    10081 taaattaccg cctttcagcc aatttgatcg agaacaattt atctcttttt gatgccaatt
    10141 tccaagactt atacattgat aaatatcaat tcctacaaat tatgttaatc atgtttcaat
    10201 aaggtaactt tagtgacatt tatgtttaaa atgtgtgagt tataaggctg aaattagttt
    10261 cggttggcaa tttaaagata aaaagagacg aagcaaaaac agcacggttg accttaattc
    10321 tttttttggt cactggctaa tacgacgcag agtttcagga ttactaataa aggcataaat
    10381 ccaccccgcc gatattaaca tttaattaac ttgtaattat attataatta actcacccag
    10441 ggttattatt ttgttaaaaa taaagccaca tatacgtggc ttgagagtga agataaacgc
    10501 aaaattacct gatgcgctac gcttgccagt gtctacatca tctctgcaat atattgaatt
    10561 tgcctgcttt tgtagaccga ataaagcgtt catgccgtat ccggcatgaa caaagcgccc
    10621 tttgtcaaca atctggccgc gcatatgtgg cttcggtttg attgctattc aggcaagccg
    10681 gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc gatagatttg aaccttgatt
    10741 ttcctcggtg gtaatgtgtt catccatcat cgtttctgag caagaaatca cctcgcgact
    10801 gaaagcaaca gtcgcatcac aggttgctga ttcagccgga ttaaacagcc gtaaaatcac
    10861 ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg catcccactg ggggcatttt
    10921 caacaaacta taactttccg gcacgttgaa tccggctttg ttgagcttca tcgcatccca
    10981 tgggattttg ttgtaacact gtactggagt cagccatgct cgcgcctgct gagctacacc
    11041 agcggcggtt ggcgtaccgg tataactcaa taaacttagg cgacaagaaa gcagaccacg
    11101 tagttgtgag tccgggactg gcattttaat tcccgaaggc cgcccaggcc ttaaaagcag
    11161 atcttctttg cccagtaagc ccacgccacg cagcaacgta atggcaaagg ttttcttctc
    11221 ttcaccgatg acttcaaatt cacgtaaccc ttcgctaaag acagccatgc cgttacgccc
    11281 ttcctgtaag gcaacatagt tgagcatatt ccataccgga accggcgctt ctttccagcc
    11341 ttcttgctgc cagttgttca ttgcactgtc gttcacgggg cgcgttagcg aaccaaactg
    11401 cgtatctgcc agaacactgt cagtgttaaa aggtgtaggg accaggacac gaaggcgatg
    11461 atcgtcagcc tggttatcaa ggttgatatc cacatcaata cgcctgctgt tatgactaag
    11521 agtgacaacc aacacccccc ctactctgcc agtggattgc cgggcgctgc gttctgacaa
    11581 attgagcggc actgccatgt catagcggat aacagccctg ctctgccagg cttcatgaat
    11641 aatatcgcat tgcggtttcg cgttcgctgc ggtaattacc cactcttctt ttgcgggtga
    11701 atagtcatat tcatcaccat catcagagct ttcttcaatt tgcaataccc ggtcatagcg
    11761 cacaccgctg tctttatcta ccagttgcag agaaccatcc tcattgagcg caatctgcca
    11821 gaaagcattt tccagaatcc cttcagcgtc actttttgcc gcaatcacgt tgccaggctg
    11881 attcgcttca atataaagcg tgcgatagcc catagaaggg acaatctggt tgatctgaat
    11941 atcaaactcc ataaagggat cataattgcc ataatgaaca atttgccgat cgattaggcc
    12001 aggatcgatc tcacgggcat ggcgaataaa atacggtaca ggctgaccgc gatcgtcttg
    12061 tagattaaac tggctggcgc gcagccgcac agtggtgtta ataacttctt cccgcggcca
    12121 gggcatcagg ttaaacagca cgagtttgtc ggcatcactc tgtggcatgt tatcggcaat
    12181 tttgcgcatg tagaaacgca gcagattatc cgccatgtct tcagccagtt cgaagcgggc
    12241 gacaatttcg cgatgaactt tgtcactgca acaacaaccg atactgtcgt gggcatgatt
    12301 ttttaagatc tctttccaca ttttctccag caagccgtgg tggtattcaa aacccaacgt
    12361 ccaggccagc gttgccagcg gttccagcag attaacaatc ttattttcaa tacgtgcgtg
    12421 ggcaattttg atatccatgc gcgtggaacc gatggtgcga tgtacgcgca tatatttgcc
    12481 atcgataaat tcacctttca gggtggccag actttcacgc tgcgcttcga tcttctcaaa
    12541 tacctcttca aagcggctca tcacaaactt acgttgaggg tagatctcac gtagcttatc
    12601 catcacttcg aagatatttt gctgcaatgg catctggtca tgaccattcg gcagcaaaat
    12661 ctctttggtt acagacgctt tttccagcac gtcaaaataa ctgtcgaggc gtttacgtaa
    12721 tccgttttcg tcggcaggta agtacttacc gatggcgtag cccagcggca gcacctgcgc
    12781 cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg gttttatcag taccgtggcg
    12841 ctccgaacat ccgcgccaga acatagtgcg ggtaatgcca aatccattgt aaatatgcgg
    12901 cagttgcccg gacatgccaa aggaatccgg taaataacct attttcatcg gctcgccaaa
    12961 cgcgaggcag tcacgcattc cgtacatcag attacggacg atggattccg cagaaacaat
    13021 cgtggtatcg gtctgggtat accaggggcc gataatcaac ttaccggctt ctacctgttt
    13081 cttcacgcgg tctttgtttt ccggtttcac cgcgaaataa tcttcgagga tcgccgtttg
    13141 cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc agtcggcaca ggatctcttc
    13201 catattattg accagcagaa tgcgtgactc ttcggtggtg aaataccact cccgatccca
    13261 atgcatatgc ggggtgatgt gaacgcgaga taccgctttc atcgtcgttt cctttttgtt
    13321 tatggcatta cgccatcagt cagatagttg ccatgcttaa ccgcgtgacg ccgccagatc
    13381 aggagaattg cagtcgagat tgcagccccc accaatgccg caccaaacca gccaattgcc
    13441 gccataacac cgcccgcgcc attatcatga agtaaaaaga gcgagaaaat gccggcaccg
    13501 ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc ccgttaccat agagcccagc
    13561 acaaacgaac caataacccg cagcggatct tcaatcgcca tcgggatcgc cccttcggta
    13621 atacctgcca gccctaacag ccaggtggat ttcccggtct caatttcaaa ctctttaaac
    13681 aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg ccgaaaccat tttgacggag
    13741 gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg cgcccaggca gaatgcataa
    13801 gcggctttgt tcactggccc tccaaggtca aaggaacaca taaaaccgag aatggcaccc
    13861 agcaacagcg cgttacttcc tgacagaccg ttcagccagg cggtaagcga gttattgatc
    13921 catgcgacag gttctcccac cacaaacagc atcagactgc ccgctcccaa cgtaccgagc
    13981 accgggtaga gataaaaagt caggaatcca ttgaatttac tgctaagacg caagtgattt
    14041 ttcacccagc gcatcaagta accggctatc aatccgccaa cgaccgcgcc gagaaacccg
    14101 gagccgatca tgttggcggc aagtccagcc gcaaagcctg gcgctaacgc cggtttatct
    14161 gccagagaat aggcggtata ggccgcgagc accggtacca tcaaaattcc gagcagcccg
    14221 ccgcccagct tgcggtacat ccacagccag gagttttctt cattaaacag atcttgtagc
    14281 ccgaagattt gcgacagtaa taccgcgacc gccagcaccg tgccccccgc gacaatcaac
    14341 ggtacggcaa aagagattcc gctcaacagt gcctgtttca gctccgtttt gacacttttc
    14401 accggttgcg tatcttgctg tacggtacgc gtctcatcgc tacgcttgag ggtaagcgct
    14461 tgttggatca acgcttctgc atggcgaatc ggctcggcaa caggcactga aagcgcggga
    14521 atgccattaa aacgctcact ctccttgatg gcgacttcag ccgcaaaaat acaggcggtt
    14581 gcactattga gttgatccgc cgttaaacgc ccttcaatgc cgttagcgcc ttgtttttca
    14641 acgtaaacat ttacgccgag tttgcgtccg gctttttcca gatattccgc agccatatag
    14701 gtgtgagcaa tacccgccgg acaggccgtt acgcagacga tagttggcgc gttggaaaaa
    14761 gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg agagcaactc atcaggcgtc
    14821 gtcgccgact gtatacgcgc ccgaatctca tcatccgcaa ggcgcgtggt cagcgctgtc
    14881 agcagttgca tatgcgtagt acccgcttca ttggggggaa tcgccagcag caccactaaa
    14941 tcaactgctt ccgggccatc aacgccttcc cactgaagcg gctcgctgag tgtggcgacc
    15001 gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg gcacagccaa cccttcacct
    15061 aaggcggtcg ggccaaggct ttcacggcga tacacttctt ccagaaattg ctcagtactg
    15121 gaaattttcc ccagagcagc aagacgttga gtcaacgcgt ggatggcctc ttcacggctg
    15181 gtaaagcgcg cattcagaca caacgcatcg cggtgggtca gagtcgtcag attcatcatg
    15241 atcctcactt gatcgttttt atagtcgcgc cagtgtgccc gataaaacaa taccatcatg
    15301 tgatcaattt cacttgattg aaattatttg tattgtattt gtattattta caggatgaat
    15361 ttatcgggca taatggctaa attcgtatta atgagatact aaaaatggga cacaagccct
    15421 tataccggca gattgccgat cgcattcgtg aacaaattgc gcgtggcgag cttaaacctg
    15481 gcgatgcgtt acccactgaa tcagcgctac aaacagagtt tggcgtcagc cgggttacgg
    15541 tgcgtcaggc tttgcgccag ctagtcgagc agcagatcct cgaaagcatt cagggcagcg
    15601 ggacttacgt caaagaagag cgggtcaatt acgatatttt tcagttaacc agttttgatg
    15661 aaaaactgtc ggaccgtcac gtcgatacac acagtgaaat cctgatattt gaagtgattc
    15721 cggctgacga ttttcttcag caacagctac aaatcaccgc gcaggatcgt gtgtggcatg
    15781 tgaagcgcgt gcgctatcgc aagcaaaagc caatggcgct ggaagaaacc tggatgccgc
    15841 ttgcgttgtt cccggatctc acctggcagg tcatggaaaa ttcgaaatat cactttattg
    15901 aagaagtgaa gaagatggtt atcgatcgta gcgaacagga aatcattcct ctgatgccaa
    15961 ccgaagagat gagccgtctg cttaatatca gccagacaaa gccgattctt gaaaaagtat
    16021 cgcgtggata tttagttgat ggtcgggtgt ttgaatatag ccgcaacgct tttaataccg
    16081 atgactataa atttaccctg atagcgcagc gaaaatcatc gcgataagca caaaaaaggc
    16141 catcatacga tggccaacca tgtcgaaacc gacagccctt cgccattcct gtagagggaa
    16201 gcggcgaggg ctatttctta ttacagatat ttatttcaga acagttttca gtgcttcacc
    16261 gatatccgcc aggctgcgaa cggttttcac gcctgcggct tccagagcag cgaatttctc
    16321 atccgcagtc cctttcccac cagcaatgat cgcacccgcg tggcccatac gtttgccttt
    16381 cggcgcagtc acaccagcga tgtaacctac aactggcttg gtaacgtgct ctttgatgta
    16441 cgctgccgct tcttcttcag cgctaccgcc gatctcaccg atcatcacga tcgcttcggt
    16501 ctgcggatct ttttcgaaca tttcgaggat gtcgataaag ttagagcctg ggatcgggtc
    16561 accgccgata ccaacacagg tcgactgacc gaaaccgtaa tccgtggtct gtttaaccgc
    16621 ttcataggtc agtgtaccgg aacgggaaac gatacccact ttacccggtt tgtgaatgtg
    16681 acccggctgg ataccgattt tgcattcacc cggagtgata acgcccgggc agttcgggcc
    16741 gatcatacga acgccggctt cgtccagttt cactttcaca gtcagcatat ccagcgtcgg
    16801 gatgccttca gtgatggtga taatcagttt gatgcctgca tcaatagctt ccagaatgga
    16861 gtctttgcag aacggtgctg gtacgtagat aacagaagcg gtagcgccag tagcagcaac
    16921 agcttcacgc acggtgttga acaccggcag gccgaggtgg gtagtaccgc ctttacctgg
    16981 ggttacgccg ccaaccattt tagtgccgta tgcaatggcc tgttctgagt ggaaagtccc
    17041 ctggctacca gtaaagccct ggcagataac cttggtgttt ttatcaatta aaatggacat
    17101 tatttcccct ccactgcggc aacaacctgc tgagctgcat ccgtcagacc ttttgctgca
    17161 ataatattca ggccgctgtc agccagtttc ttcgcgccga gttcggcgtt gttaccttcc
    17221 agacgtacca cgaccggtac gttaacaccc acttctgcta ccgcgccgat gataccgtca
    17281 gcgatcaggt cgcaacgaac gataccgccg aagatgttaa ccagaacggc tttcactttg
    17341 tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt ctttggttgc gccgccgcca
    17401 acgtcaagga agttagccgg ttcgccgccg tgcagtttaa cgatgtccat cgtacccatc
    17461 gccagacctg cgccgttaac catacaaccg atgttaccgt ccagcgcaac gtagttcagt
    17521 tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg actggtcacg catttcgcgc
    17581 agatcaggct ggcggaacag tgcgttgccg tcagcgccca gtttgccgtc gaggcaaatc
    17641 agatcgccct gtttggtgat gaccagcggg ttgatttcga tcaacgccag gtcgcgctcc
    17701 aggaaaatgg tcgccaggcc catgaagatt ttggtgaact gctgaaccag tttaccttcc
    17761 agacccagtt tgaacgccag ctcgcgtccc tgatacggca tcgggccagt cagcggatca
    17821 agcgcaactt tgtggatcag gtgcggagtt tcttccgcca ctttttcgat ttccacgccg
    17881 ccttcggtgg aggccataaa gaccacacga cgggaactac ggtctacaac ggcaccgaga
    17941 tacagctctt tagcgatatc ggtcgctgct tcaaccagaa tctggttaac cggttggcca
    18001 ttggcatctg tttgatacgt taccagacgc ttgccgagcc agttttctgc aaaagcacgg
    18061 atgtcttctt tgctgtttac aactttcaca ccgcccgctt taccgcggcc accagcgtga
    18121 acctgacatt tcactaccca cgggccggca ccgatttttg aagcggcttc ttctgcttcg
    18181 cgcggagtag tacaggcata acccaccggt gctggtaagc catagcgggc aaaaagttgt
    18241 tttgcctgat attcatgtaa gttcatgtgt tctgtccatc cttcagtaat cgttatcttt
    18301 taaaccgtag accgacaccc tatccacgtc ggtctacagt gcaggtgaag cttaaactac
    18361 tacacgtcca gcagcagacg cgtcggatct tccagcaact ctttgatcgt taccaggaag
    18421 cccacggatt cgcgaccatc gatcagacgg tgatcgtagg acagcgccag gtacatcatc
    18481 ggcaggatct caacctgacc attcaccgcc atcggacgat ctttgatagc gtgcataccc
    18541 agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca tcagggaacc gaacacacca
    18601 ccgttggtga tggtgaagtt accaccggtc agatcttcaa ccgtcagctt gccgtcacgg
    18661 cctttaactg ccagctcttt gattttcttc tcgatgtctg ccatgccgag ggtatcgaca
    18721 tcacgcagta ccggcgtcac caggccgcgc ggcgtggaaa ccgccatgct gacgtcgaaa
    18781 tagttgtggt aaaccacgtc atcaccgtcg atagaagcat tcacttccgg gtaacgtttc
    18841 agggcttcaa ccaccgcttt cacgtagaag gacataaagc ccagacggat gccgtggcgt
    18901 ttttcaaacg cttcaccgta ctgcttacgc agatccataa tcggcttcat gttgacttcg
    18961 ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca gcagacgctc tgccacacgc
    19021 ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg cagccagagc cggttgcgcc
    19081 gccggagcag ccgctgccgg agcagactct ttcgccgggg ctttcgccag atgtttttcc
    19141 acatcttcac gagtcagacg accacccaca ccggtgcctt taatggcgct ggcgtcgaga
    19201 ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg catcgttgtt ttgctcttcc
    19261 agagacgcct gctggcgttg cgccggagtg gacgctttct cttcagattt ggcgctggtt
    19321 tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga tctgacgaga cgttaccgtt
    19381 gtaccttcat cttccagaac cgcatccaga atgccgtctg ctgatgccgg tacttccagt
    19441 accactttgt cagtttcgat ttctaccagc acttcatcac gtacgactgc gtcgccgggt
    19501 tttttatgcc aggttgcgac ggtggcatcg gctacggatt caggcaggtc agggaccaga
    19561 atatctacgc tactcattgt gtatccttta tttattcgac gttcagcgcg tcattaacca
    19621 gatcttgttg ctgtttctgg tgaacggaca tataccctac cgccggagag gcggaggccg
    19681 tgcggcctgc ataacgcaga gaagccccaa acggaatcac ttcacggaaa tgatgctggc
    19741 tgcagtacca tgcgccctgg ttgagcggct cttcctggca ccagacaaaa tccttgacgt
    19801 gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg gaacgggtag agttgctcga
    19861 tacgcacaat ggcgacatcg tgttgattgt tcttacgacg ctgttccagc aggtcgtaat
    19921 aaaccttacc agaacacatc actacgcgct tcacgccctt cggatcaagc tcgtcgattt
    19981 caccgatggc tggcaggaag gtgccgttcg ccagttcttc gaggctggaa accgccagcg
    20041 gatgacgcag cagggatttc ggcgacatca ctaccagcgg acgacgcatc ccgcgcagcg
    20101 cctgacgacg cagcatgtgg taaacctgtg ccggggtaga cggaacgcaa acctgcatgt
    20161 tttgctcagc acaaagttgc agataacgtt ccagacgcgc ggaggagtgc tccggcccct
    20221 gcccttcgta accgtgcggc agcaacatca ccagaccaca catccggccc catttctgtt
    20281 cgccagaaga gatgaactgg tcgataacca cctgcgcacc gttggcgaag tcaccgaact
    20341 gcgcttccca gatggtcaga gtgcgtggtt ctgcggtggc ataaccgtat tcaaacgcca
    20401 gcactgcttc ttcagacagt acggagtccc agacgcggaa cgcgccctgc ccgttatgga
    20461 tgtgttgcaa cggcgtgtaa gtggaaccgt tagactggtt gtggatcacc gcgtggcggt
    20521 ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg aaccggaatg ccttcgtcaa
    20581 ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc gaacagtttc tcaccggcag
    20641 ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga ctgcatttca actgcttccg
    20701 gcaccgtgct gatgcgtttc gccagctcct gcaggcgctt catctcaact ttgttcgggt
    20761 actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt gaaagagtgc atgttcatcg
    20821 gacgccactc tgccactacg caatcgccag catccagcgc atcgcggtac aggttaacca
    20881 tctcggtggc atcttccagc gtcgccactt tttcctgctc cagcttgtca gcgtagattt
    20941 tgcgcggtgt cggatgtttt ttgattttct gatacatcag cggctgggtt gcgctcggct
    21001 cgtcggcttc gttgtggccg tgacggcggt agcacaccag gtcgatgaag acatcacgtt
    21061 taaaggtgtt acggaaatcg agcgccagac gggtcacaaa ggcaacggct tccggatcat
    21121 ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc gatatcagta cagtacggtg
    21181 tagaacgagc atccagtgga ttagaggtgg tgaaaccaac ctggttgttg ataacgatac
    21241 gtaccgtacc gccaacttca taaccacgcg ctttcgacat gttcagagtt tcctgaacca
    21301 cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat tggcagcact ttgttgctgc
    21361 tcggctcatc aagtctgtcc agacgggcac gaacagaacc gataactacc gggcttacaa
    21421 tctcaaggtg agacgggtta aacgccagcg ccaggtgcac caggccgcca tcggtctgga
    21481 agtcagacga gaagcccatg tggtatttca cgtcacccgt gccgaggtgt tctttatgtt
    21541 taccggcaaa ctcgtcgaac aagtcttgcg gttttttacc cagcacgttc accagcacgt
    21601 tcagacgacc acggtgcgcc atcccaagaa ccacttcgcg ggtgccgctg ttgccagcgt
    21661 ggcggatcat ctctttaagc atcgggatta acgcgtcacc gccttccagc gagaagcgtt
    21721 ttgcgccagg gaattttgcg ccgaggtaac gttcaaggcc ttcagcggcg gtcagttcgc
    21781 ttaagaagcg ttttttctct tcgctattga aagtcgcgcg accagactcg atacgctgtt
    21841 ggatccagcg tttttcttcg gtgctggtaa tgtgcatata ctcggcacca atcgggccac
    21901 agtaggtttg cttgagggct tccagcagct cgccgagttt catgttttct ttgccgctgg
    21961 caaatgaacc gacgttgaag gtctcctgga agtctgcttc ggtcagatcg tggaaagacg
    22021 gatccagatc ggccacttta tcttgctgcc acagtcccag cggatcgaga ttcgcatgct
    22081 ggtgaccacg gaagcggtat gcgttaatga gctgcaggac tttaacctgc ttcacattgg
    22141 tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc tttcgccagg cggcggaaat
    22201 attcacgcgt ttgagagtgg aattgatccg gtttgactcc cgtaccaggt aactgctgga
    22261 acgtcgaacg ccagttagcg tcaaccgagt caggatcggt taagaagtct tcatagagct
    22321 gttctatcca gctctggttt gcgccagaga ggtaagaaga gtccaaccag gctttcaaag
    22381 cgctgttctg catcgtgatc ccttaagcat cttttttatg cttacttcgc cgtggatact
    22441 accacgcaca gtgatgtgcg tgccggggtt cacttgcgag ctcttgtcta tgtagtggct
    22501 cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca gtttttaaag atttcctgca
    22561 tttctttgtt acgcctgatg cgctgcgctt atcaggccta cgggtaatga cttgtaggcc
    22621 ggataaggcg ttcacgccgc atccggcact ggttgcctga tgcgacgctt gcgcgtctta
    22681 tcaggcctac ggtttacgca ttacgttgca acaacatcga cttaatatgg ccgatggcgc
    22741 gcgtcgggtt cagccccttc ggacatacac tgacgcagtt catgatgctg tgacagcgga
    22801 atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc agtctcggta tcacggctat
    22861 cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc gataaactta tcgggattcc
    22921 accagaaaga cggacaagag gttgaacaac atgcgcagag aatacattca tacagcccgt
    22981 cgagtttttc gcgctgctct ggcatctgta aatgctcgcg agctggcgga ttttgtccat
    23041 tattcaacag gtaaggctta attttctcat attgcgcata gaattgtccc atgtctacca
    23101 ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat cacaatcttc ttgcccggct
    23161 ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt cttgccgttc atgttcagac
    23221 cgtcggaacc gcacacacct tcacggcagg agcggcggaa cgacaggctg ggatctttct
    23281 ctttcagctg gataagcgca tccagcagca tcatgtcgcg accttcttcc gcttccaggg
    23341 tgtaatcctg catacgcgga gcatcatcaa catccgggtt atagcgataa attgaaaact
    23401 cgagtctcat tttcctgtct ccgcattagt aagtacgaat cttcggcggg aatgccgggc
    23461 gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga ttccgactct ggcagataca
    23521 gggagtggca cagccagttt tcatcatcac gatccgggaa gtcgaagcgg ctatgtgcgc
    23581 cacggctttc ggtacggaag ttggcagaaa cagccgttgc atacgccgtt tccatcaggt
    23641 tatccagttc caggcactca acgcgctggg tgttgaactc gctggaagtg tcatccagac
    23701 gggcattttt cagacgctcg cggatcactt tcaactgctc aagccctttc gccatcgcat
    23761 caccttcacg gaagaccgag aagttatgct gcatacattc ttgcagcgct ttacgaatcg
    23821 ccaccggatc ttcaccgtta cgattattgt tccagcggtt cagacgatcc agagatgctt
    23881 caacatcaga ctcgctggca tcgcgcaatg cgccctgttc ggcgatagac tcttgcagat
    23941 gcagacctgc cgcacgacca aagaccacca ggtccagcag cgagttgccg cccagacggt
    24001 tagcgccgtg taccgataca caagcgattt caccaacggc aaacagcccc ggaacaacca
    24061 catcttcgcc tttctcgttc accgtcagcg cctgaccggt cactttggtc ggaataccgc
    24121 ccatcatgta gtgacaggtt gggataaccg gaatcggctc tttcaccgga tcgacgtgag
    24181 caaaggtacg ggaaagctcc aggatacccg gcagacggga ttcgagaact tctttaccca
    24241 ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc atcacagccg cgaccttcac
    24301 ggatttcaat catgatggaa cgcgccacca catcacggcc cgccaggtct ttggcgttcg
    24361 gcgcataacg ctccataaag cgttcgccat gtttgttcag cagataaccg ccttcaccac
    24421 ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc ggtcgggtgg aattgccaca
    24481 tttccatatc ctgcaccggt acgcctgcgc ggatagccat gccgacaccg tcgccagtgt
    24541 taatgtgggc gttagtggtg gactgataaa tacgccctgc cccgccagtc gccagcacgg
    24601 tagcgcgggc tttgaaataa accacttcac cggtttcgat gcacagtgcg gtacaaccca
    24661 ccaccgcgcc atcctggttt ttcaccagat ccagcgcata ccactcggag aaaatggtgg
    24721 tgtggttttt caggttctgc tgataaagcg tgtgcaacag tgcgtgaccg gtacggtcag
    24781 ccgccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt tttcgactga ccgccaaacg
    24841 gacgttgata gatacgacca tcatcaagac gcgagaacgg caggcccatg tgttcgagtt
    24901 ccagaatcgc ttccggcccg gttttacaca tatattcaat cgcgtcctgg tcaccgatat
    24961 agtccgaccc ttttacggtg tcgtacatat gccattccca gttatcttca tgggtattac
    25021 ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg ggaacgggtc gggaagactt
    25081 tagagagcag cgcacaggtc tggccgctct gggaaatttg cagcgccgcg cgcatacctg
    25141 cgccaccggc accaatcaca actgcatcaa attctctgac tggcaatttc atcacacacc
    25201 ccacaccaca acgaatccat aaatcacgta aaccaccagt gcaacgacaa tcaccagttg
    25261 cagcatcagg cgcaatgcca gcggtttaac gtagtcggtc aacacctgcc acatgccgat
    25321 ccaggcatga atcaagatag aaaacagcgc cagcagggtg aacactttgg tgaacgcaga
    25381 ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca ctggtggcga aaaaaccgac
    25441 catataaatg atgtagagcg tcaggacgat agcggtagcg cgaacgagga tgaaatcatg
    25501 tacgccattg cgtcctaatg cggaggcgtt gcttaccata cgaggactcc tgcgagaagt
    25561 gaaagcacga cagtaataac aaaggagatt ttggcggagc gtttacccgc ttcgaatgtt
    25621 tcttccagat agccaaaatc catcatcatg tggcgaatac ctacgacgac gtgatacgcc
    25681 agagcggtaa ggatgcccca catgataaat ttgacgaaga agctgcccat aatcgcggaa
    25741 gcttgctcga aaccttcagg ggaagagagg ctggtaccca gaagccacag caggatgccc
    25801 actgcaacaa aggtgatcac accggaaacg cgatggagaa tggacgctat cgccgtgatg
    25861 gggaaccgga tggtctgtag gtccagatta acaggtcttt gttttttcac atttcttatc
    25921 atgaataacg cccacatgct gttcttatta ttccctgggg actacgggca cagaggttaa
    25981 ctttctgtta cctggagacg tcgggatttc cttcctccgg tctgcttgcg ggtcagacag
    26041 cgtcctttct ataactgcgc gtcatgcaaa acactgcttc cagatgcgaa aacgacacgt
    26101 tacaacgctg ggtggctcgg gattgcaggg tgttccggag acctggcggc agtataggct
    26161 gttcacaaaa tcattacaat taacctacat atagtttgtc gggttttatc ctgaacagtg
    26221 atccaggtca cgataacaac atttatttaa tttttaatca tctaatttga caatcattca
    26281 acaaagttgt tacaaacatt gccaggaaaa gcatataatg cgtaaaagtt atgaagtcgg
    26341 tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac
    26401 agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta
    26461 ttcgtcatca atggatcctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc
    26521 tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc
    26581 aaatttaagt tccggcagtc ttacgcaata aggcgctaag gagaccttaa atggctgata
    26641 caaaagcaaa actcaccctc aacggggaca cagctgttga actggatgtg ctgaaaggca
    26701 cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg
    26761 acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg
    26821 aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc
    26881 tggaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat
    26941 ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccatg
    27001 ctttccgtcg cgactcgcat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg
    27061 cgttctatca cgactcgctg gatgttaaca atcctcgtca ccgtgaaatt gccgcgttcc
    27121 gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc
    27181 catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct
    27241 ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc
    27301 tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct
    27361 cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg
    27421 cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac
    27481 acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct
    27541 tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc
    27601 atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg
    27661 aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt
    27721 tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt
    27781 tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac agtgacggta
    27841 tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg
    27901 atatcaagcg ttaatggttg attgctaagt tgtaaatatt ttaacccgcc gttcaaatgg
    27961 cgggttaatt tttatatgcc caaacacaaa aaattgtaaa aataaaatcc actatcggac
    28021 attcatatat tctaaaaaaa atatatcggt ataaattata aacaaatcga tgatttttaa
    28081 tcatctatct tcctctcagt tacatgaata aaccaccaaa tatagcgcac ccctataatt
    28141 tccaatactt tattcatcaa acatttaatt cataccatca attaattact tttacattta
    28201 ataaaattaa taatattttt aagaaaaact aaaaacattc gacaatcaaa taagatattc
    28261 ttgcaccgat atactttttt cttttctgtt aaaggactca gttatgttta aaggacaaaa
    28321 aacattggcc gcactggccg tatctctgct gttcacagca cctgtttatg cggctgatga
    28381 aggatccggt gaaattcact ttaaaggtga agttattgaa gcaccgtgtg aaatacatca
    28441 ggatgatatt gataaagagg ttgaactcgg tcaggtgacc accagccaca ttaatcagtc
    28501 acatcacagc gatgccgttg ctgtcgacct gcgcttagtc aactgtgatc tggaaaactc
    28561 cagcaacggt tccggtggca agatttctaa agttgcagtc acctttgata gctcagcgaa
    28621 aaccaccggc gcagatccga ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg
    28681 cgtacgttta atgaataaag atcaaagcaa tatcgtttta ggcaccgcta ctccagatat
    28741 cgacctggct ccgacctcca gcgaacaaac gctgaatttc tttgcctgga tggaacaaat
    28801 tgatcaggct acacctgtaa cgccaggcgc agttacggcg aatgcaacct acgtgctgga
    28861 ttataaataa gtattacttt ggcggcgcta tcgccgcctt tcataaaata tttatcaggt
    28921 atggacaccg tgaatattta tcgactctct tttgtatcct gcctggtcgt ggcgatgcct
    28981 tgcgcattgg cggtcgaatt caaccttaat gttctcgata aatcgatgcg cgaccgcatt
    29041 gatatttcat tattaaagga aaaaggagtc attgctcccg gtgagtattt tgttagcgtt
    29101 gcggtaaata ataaccaaat cagtaacggg caaaagatta actggcacaa aaatgacgat
    29161 aaaaccattc cgtgcatcaa tgatttactg gtcgataaat ttggcttaaa acctgaagtc
    29221 cgtcagtcgt taccattgat aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc
    29281 ttcaatttcg atcaagccaa tcagcaactg aatattagta ttccgcaagc ctggctggcg
    29341 tggcactcag aaaactggac cccaccctcc acatggaaag aaggtgtcgc cggtatcctg
    29401 atggattaca acttgtttgc cagcagctac cgcccacagg acggcagcag cagcactaac
    29461 ctgaacgcct acggtaccac cggaattaac gccggggcat ggcgcttacg tagtgattat
    29521 cagttgaatc agactgatag cgatgataac catgaacagt caggcggaat atcgcgcacc
    29581 tatctttttc gtccattacc gcaattaggc tctaaattaa ccctcggcga aacggatttt
    29641 agttccaata ttttcgacgg tttttcttat accggcgcgg cactggcaag tgacgagcga
    29701 atgttgccat gggaactacg cggctacgcc ccacaaatta gcggtattgc acagaccaat
    29761 gccacggtga cgatcagtca atcaggccgc gtcatttacc agaaaaaagt cccaccaggc
    29821 ccatttatca ttgacgacct taatcagtct gttcagggca cactggatgt caaagtgacg
    29881 gaagaagatg gtcgggtgaa caatttccag gtttcggcag catcgacgcc cttcctgact
    29941 cgtcagggac aggttcgcta taaactggcc gcgggtcagc cacggccctc catgtcacat
    30001 caaactgaaa atgaaacctt ttttagcaat gaagtttcct gggggatgct gtcaaacacc
    30061 tcgctgtacg gcggcctgct gctttctggt gatgactacc attctgccgc aatgggtatt
    30121 gggcaaaata tgctgtggct tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat
    30181 tttgatactc agcaggacga gcggggctta agctaccgtt ttaattacag caaacaagtg
    30241 gatgccacta acagcacgat ttccctcgcc gcttatcgtt tctccgatcg tcattttcac
    30301 agctacgcca actatctgga tcacaaatac aacgacagcg atgcgcagga cgaaaaacag
    30361 acgatcagct tattgtgggc caaccgatta ccccactaaa cctcaatctt tacgccaacc
    30421 tgctacatca aacctggtgg aatgcagacg cctccacgac cgccaacatc acggcaggtt
    30481 ttaatgttga tattggtgac tggagagata tctctatttc gacgtcattc aatacaaccc
    30541 attacgaaga taaagatcac gacaaccaga tttacctgtc gatttcgctc cccttcggta
    30601 acggtggtcg ggttggctat gacatgcaaa acagtagcca cagcaccaca caccgcatgt
    30661 cgtggaacga tacgctggat gaacgtaata gctggggcat gtctgccgga ctgcaatccg
    30721 accgtcctga caatggagcc caggtgagcg gtaactatca gcacctgagt tcagcgggtg
    30781 agtgggatat ttctggtacc tatgccgcca atgattacag ttccgtcagc agcagctgga
    30841 gcggttcttt caccgcaacc caatatggtg cagcgtttca tcgccgcagc tccaccaatg
    30901 aaccacgcct gatggtcagc accgatggcg tggcagatat tccggttcag ggcaatctcg
    30961 actacaccaa ccattttggc attgcggtgg tgccgttgat ttccagttac cagccttcca
    31021 ccgtggcggt gaacatgaat gacttacccg acggcgtaac agttgcagaa aacgttatca
    31081 aagaaacgtg gattgaaggc gcgataggtt acaaatcact ggcttcccgt tccggtaaag
    31141 acgttaacgt catcattcgc aacgccagcg gtcagtttcc tcccctcgga gcggatatcc
    31201 gccaggatga cagcggcatt agcgtgggga tggttggcga ggaaggacat gcctggttaa
    31261 gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg tgatagccag cattgctcgc
    31321 tccatcttcc tgaacatatg gaagacaccg caaatcgcct gattttacct tgtcattaat
    31381 taaggaaatg aaaatgacat ttatgaaagg actacccctt ttgttgttgg ttgccagtct
    31441 gtgcagccac gctgcactac aacccgatcg cactcgtatt gtgtttaacg ctaatgataa
    31501 agctaccagt ctgcgggtgg ataatcgtag cgataaactc ccctacctcg cttattcctg
    31561 gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc cgccgataca
    31621 acgtctggaa ccgaaagcaa caacgcaggt acggatagta aagcaagcct cgaccacaaa
    31681 attaccgggc gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc ctcccgcccc
    31741 ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt
    31801 ctggcgaccg gctgcattac gcaagaaagc aggagaaaag gtcgaactgc agttacaggt
    31861 cagccagcag ggcaaccaac tgacactgaa aaatcctacc gcgtattatc tgaccattgc
    31921 ctacctggga cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc
    31981 ttttagtacg gtcaacacaa acaccggaaa ttatagcggc agccagtttt atctcggtta
    32041 tatggatgat tacggcgcgt tacgcatgac gacgctcaac tgcagcggac aatgccattt
    32101 acaagccgtg gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt catctgtctg
    32161 ttatttctgc cgttgaagtc tgcgcttgcc ctgaactgct actttggttc atccggtggc
    32221 tcggtagaaa aatcagaagc gattcaacct tttgctgtac caggcaatgc caaactcggc
    32281 gataagattt gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac
    32341 ggcaattttg aaagtgagca tgtttatgcc tgggtaaccc cctacccggg ggtgcaggat
    32401 cgctattatc aactcggcgt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa
    32461 agccgcatcg acactaacca gtgtattgac agtaagaaca ttgacattta tacccctgag
    32521 cagattatcg cgatgggatg gcagaacaaa atttgctcgg gtgatcccgc taatattcac
    32581 atgtcgcgta cctttctcgc tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg
    32641 catgattatc aaagtacgct tagcgactat atcgtcgtgc aatttgacgg tgccggtagc
    32701 gttaacgaag accctactgc ccaaaacctg aaatatcata ttactggtct ggaaaacatt
    32761 cgcgtgctgg attgcagcgt caatttttcc atttcaccgg aaacacaggt gattgatttt
    32821 ggtaaattta atttgctgga tattcgtcgc cacacaatgt cgaaaacatt cagtattaag
    32881 acgacaaaat cacaaaatga ccaatgcacc gacggattta aagtcagttc ctccttttat
    32941 accgaagaaa cgttggttga agaagataaa gcattgttga ttggtaatgg tctgaagcta
    33001 cgtttgctgg atgaaaacgc ctcgccttat accttcaata aatatgctga atacgccgat
    33061 ttcaccagcg acatgttggt ctacgaaaaa acctatacag ctgaactttc gtccattgca
    33121 ggtaccccca tcgaggctgg gcccttcgat acagtggtgc tttttaaaat taactataac
    33181 tgatttgcca tgatgccctt tgcagccgga aagggcatct ctttcagaaa attgctgatt
    33241 ttcccctttt ccttgattgc tgttattttt tcctggcccg gctttcttta tgctgtatcg
    33301 atacaccaaa gagaaaataa tgagggagcg aaggatgcca gttttgcagt ggggaatgtt
    33361 atgtgtgcta tcacttctcc tttctattgg tttcctcgcg gtacatctcc cggcggcgct
    33421 attacttggg ccgatgatcg ccgggatcat cttcagtatg cgcggaatta ctctgcaact
    33481 tccccgctcc gcttttctcg ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct
    33541 cactggttcg attctcacca cgctggccgc caactggccg atcgtgcttg ctattttact
    33601 ggtgacgctg ctttccagcg ccatcgtggg ttggttattg gtgcgctata gctcactgcc
    33661 tggaaatacc ggtgcctggg gttcttctcc aggcggcgca gcggcaatgg tcgctatggc
    33721 gcaagattac ggcgcagata ttcgcctggt tgcatttatg caatatctgc gagtgctgtt
    33781 cgtcgcgggg gccgctgttc tggtcacccg catgatgctg ggtgataacg ctgaagcggt
    33841 taaccagcaa atcgtctggt tcccgccagt gagcattaat ctcctgctta cgattttgct
    33901 cgcagtcgtt gccggtacgg cgggatgcat gctgcgtctt ccttccggca cgatgctcat
    33961 cccgatgctg gcgggcgcag tgctccagtc tggtcagctc atcaccatcg aactaccgga
    34021 atggctgctg gcgatggcgt atatggcaat tggctggcgg attggtcttg gtttcgataa
    34081 acaaatctta ctgcgggcac tacgcccgct accgcaaatc ctgctgtcga tttttgctct
    34141 gctggctatt tgtgcgggta tggcgtgggg gctgacccgg tttatgcaca ttgattttat
    34201 gactgcctac ctcgccacca gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg
    34261 gagcaatgcc gatatggcgc ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt
    34321 gctgacgggg ccagccattg cgcggtttat ttcaacctat gcgccgaagc ggtcggccta
    34381 gtgctggcag ccagggcacc agtaaaacgg gcgagatgac agcgtggttt tctcaatgat
    34441 gctgccacag cgttcgcacg gttcgccatc acgatgaaaa accttaaagc gaaacagcgc
    34501 cccatgatgc ttattctcat ccacctgccc cctcgtagcg taggaaaatc gaggaatatc
    34561 cagtaacgcg tgtgcgagtg catccagttg cgccgcattg agatctttcg ctttatgatt
    34621 tccagtcaac ccaacctgcc agaggatctc cacccgcaaa taattgccaa gcccagccag
    34681 aaacgcctga tcgagcagta atcctgcaaa ctgacggtta cgaaagcgcg gtgacaataa
    34741 ccgttccttt accacctccg gcgtcagatt cggatccagc acatcgggac caacgcgttg
    34801 taaaaacgga tgcgtggtca gttgttccgg cctcaacatc tcaatatccg aggcgctata
    34861 aagcagaatg gttttgtcag ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg
    34921 ctcttcgccg gtatcaacca cgcgccagac gccgtaaagc tgattatggc tgtagagcgt
    34981 taagtcgttg gaaaaatgag ttaacaacgc cttaccacgc gtttccacat gggtaacgtg
    35041 ttgaccgata agttgtgatt gataagtttt taactgcggg aaggcaaacc agacatcagt
    35101 tagtggtttg cctttgatcg ccgcctccag gttatccgct gcacggcgga tctccgggcc
    35161 ttcaggcatg gtgatatcct tttttcattc tttattaatc ggggttatgc tgcgacaaca
    35221 atctcctttt cggcaaatgt agagcgtagt cggcgggcga acgccagtgc gtgctcgccg
    35281 tcgccatgca ggcagactgt ttgcgccgtg accgttgccc attcgccggt gatacttttg
    35341 actctgccgt gttgcaccat ctctagcgtt tgcgccaacg cctgttcttc gttttcaatc
    35401 aacgcgcctc gctgacttcg cggcaccagc gagccgtcag cctgataacc gcgatcggca
    35461 aacacttcct cgcgcgttgt caggccatat tgcttgcctg cacgaatcag ctcgcttcct
    35521 gccagcccaa cgagaatcaa tgccggatcg caagcgtata ccgctctggc gatggcgtct
    35581 gccagttgtg cttctttcgc cgcctggttg tacaacatgc cgtgcggttt gacatgacgc
    35641 attacgccgc cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg
    35701 gcgtaaacgg tttctggcgg cagctgcatg gcgctgcgac caaaattttc cctgtcggga
    35761 aaactcgggt gagcgccaat cgcgacacca ttttttattg cttcacgcac gcaagcctgc
    35821 atgatttgcg catcgcctgc atgaaatcca caggcaatat tggcagagga aaccagcgtt
    35881 aatagctctg cgtcgctggc gcagccttcg cccagatcgg cgttcaggtc aattttcatt
    35941 gtgcagccgc cacactaatt gttcgaaata acgttgctga tcttgccgcg ctttcagtgc
    36001 ctcttccagt gaacactgga caaaatgaat cggctgaccg agcggaattt gcgccagatg
    36061 gtacatatca gcctcaatga tacaggcaat acgcgggtaa ccatcggtgg tctgtgcgtc
    36121 gttcatcaac acaatcggct gcccgttatg tggcacctgc accacgcccg gtaacaaacc
    36181 gtgagataac agttcgcgat cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc
    36241 catgcggtta ctttgcgagc taagctgcca gggcgaacgc cagaatgcat cctgcgaggc
    36301 gcgatcgaac tcatgatatt ccggccccgg caaggcgcga atgcggttgc cccacagcag
    36361 ctgtttaacg ccctgcgctt ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc
    36421 accatccttc agtaaacggc cttccagccc gccaatcccc actttgagat cggtgctgca
    36481 tgaccccatt accggcggaa catcaatacc acccgcgacc gccagataac tgcgcatccc
    36541 gtgctgcggg cgtttaagcg ttaaacgctg gcctgctttc atcggcaatc gccagccggt
    36601 ccagacggca ttatcatcca gccgcgcttc gcaaccggca cccgtcagag caaaccaccc
    36661 atcagtttcg aactcaaccg ttaactgacc gagcgtgatc tccagcgcgg gggcattggc
    36721 gtcattaccc accagcaggt tagcaatgcg taacgcaggc atatccagtg cgccgcagtg
    36781 gctgattccc gactggcgaa aaccgtgacg accgccatcc tgcacagtgg tatacatgcc
    36841 cgcacgaata atcttcagca tactccctcc ttctgcggta caaagcgcac gctgtctccc
    36901 ggacgtaata agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca
    36961 atcaactgcc agccacccgg cgttgccagc ggataaacac cagtctgcgg cccgccgatc
    37021 ccgacagaac ctgccggaac gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt
    37081 tccggcaaac tcccgagata cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat
    37141 tccacggagg agtgcaattc aacaacctgt ttttcgctaa acccgcaatg cgccgcgacc
    37201 accgccaaat ccggtccgcc tgcaccaccg taaaccaccg gaatttcaat aaagcgagac
    37261 tcaggttcca gggcctcgct ctcctcccac cagcgttgca aacgctctat ggcatccagc
    37321 gccagcgact caggattacg caaaatcacc gtgatattgt tcatgccggg aatggcttca
    37381 accacattcg gcatatccac cagacgctgc gccagtcgcc agatccgttt ctggctagcc
    37441 agcgtcaccg gcggttccag ttccagcact accgccgttt cacctatcag ataacaacgc
    37501 gctcgttgca ctttcgatcc ctctgattat tcgttatgca ggattaggaa tatcaataaa
    37561 ggtcacatca agatcggtat tttcattcag ccactcgctc aatgcgcgaa taccaccacg
    37621 ttcagtggca tggtgacctg cagcataaaa atgcaacccc tgctcgcggg ctgaatgaat
    37681 ggtctgttca gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg cggcgctatc
    37741 gataaaactt tgcccgccgc ccgtgcacca ggcgacgcgc tggaccacct caggtccggt
    37801 atcgccacac cataacggct tacgtcccag acgcgcttca atccaggagg ctagttccag
    37861 tcccggcaca ggcatggtca gctctcccca cggcaccaac ggctcaattt cgcccatgac
    37921 cgtgatcccc agtaacgccg ccagctgtgc gttattgccc agctcaggat gtacgtcaag
    37981 cggcaaatgc cagccataca ggttgatatc attcgccagc aacgttttta aacggttacg
    38041 cttcattccg cgaatgaccg gagactctcc tttccagaag tagccatgat gcacaatgac
    38101 cgcatccgcg cccagacgca ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt
    38161 aacaattttt tgcaccgtct ctttgccttc tacctgcaaa ccattcggcg cgtaatcact
    38221 aatcgccgcg ctgttcagtt tttcgttaat cagttgttcc agttcggtgt ttttcatctt
    38281 cgcgccttcc gtattctgat agctacaccg ttaacatagc gttacctcgc ctgttcgtcg
    38341 atagccaatc ttttgacata ttaagaaaca aacccgacgc caaaagacgc aaattgttta
    38401 tatcatcacg cttcagctaa tgtatattta tgcaatcaga cagcatattg cataaataat
    38461 cagatctgta tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt
    38521 gacgttgcag tcaattgtgg aattattatg aataaacacg catcacaacc gcgcactatt
    38581 tactatgtcg ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg
    38641 ctgattctct atctcaccaa tcaactaaaa tacaacgata ctcacgccta cgagttattt
    38701 agcgcctact gttcgctggt gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa
    38761 gttctcggca atcgcatggc ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg
    38821 gtgctgggtg ccagtgagat ccatccgtca ttcctctatc tgtccctggc gattatcgtt
    38881 tgtggctatg gtctgtttaa atccaatgtc agttgcctgc tcggcgagct gtatgagcca
    38941 accgatccgc gtcgtgatgg cggtttctcg ctgatgtatg cagcgggtaa cgtggggtct
    39001 attatcgcac ctatcgcctg tggttacgcc caggaagagt acagctgggc gatgggcttt
    39061 ggcctggcgg cggtgggtat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat
    39121 ttcacacata cccgcggcgt taataaaaaa gtactgcgtg caacaaactt tctcctgcct
    39181 aactggggat ggctgctggt tctgctggtg gcaacgcctg cgctgattac cgtactgttc
    39241 tggaaagagt ggtcggtata cgccttaatt gtcgcgacta ttattggcct gggtgtactg
    39301 gcaaaaattt atcgcaaagc agaaaaccag aaacagcgta aggagctggg gctgattgtg
    39361 acgctcacct tcttcagtat gttgttctgg gccttcgcac aacagggcgg cagctcaatt
    39421 agcctttata tcgaccgctt cgttaaccgc gatatgtttg gttataccgt tccgactgct
    39481 atgttccagt cgattaatgc cttcgcagtt atgctatgcg gtgtgttcct ggcgtgggtg
    39541 gttaaagaaa gcgtcgcagg taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc
    39601 cttggcctga tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg
    39661 tatggtcact cttctctgcc actgatggtg ttaggcctcg cggtgatggg ctttgcggaa
    39721 ctgtttatcg acccggttgc catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc
    39781 ggcgtattaa ccggcatcta catgctgctt tctggcgcga tcgcgaacta tctggcgggc
    39841 gtgattgccg atcagacatc gcaggcttcg tttgatgctt ccggggcgat caactactcc
    39901 atcaatgcat atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga
    39961 ctggtactga tgatttggct gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg
    40021 gagtcataat aggccagcac cgatgcctga tgcgacgctt gacgcgtctt atc
//
LOCUS       DAQSWQ010000029        39411 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.029, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000029 DAQSWQ010000000
VERSION     DAQSWQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.029"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..266
                     /locus_tag="R8G18_001566"
     CDS             <1..266
                     /locus_tag="R8G18_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEE9795292.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="R8G18_001567"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="R8G18_001567"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HEE9795293.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="R8G18_001568"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="R8G18_001568"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEE9795294.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="R8G18_001569"
     CDS             complement(1570..3270)
                     /locus_tag="R8G18_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HEE9795295.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="R8G18_001570"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="R8G18_001570"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HEE9795296.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="R8G18_001571"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="R8G18_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="R8G18_001572"
     CDS             4828..6600
                     /locus_tag="R8G18_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HEE9795297.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="R8G18_001573"
     CDS             6593..8374
                     /locus_tag="R8G18_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HEE9795298.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="R8G18_001574"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="R8G18_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEE9795299.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="R8G18_001575"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="R8G18_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HEE9795300.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="R8G18_001576"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="R8G18_001576"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEE9795301.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="R8G18_001577"
     CDS             11336..11908
                     /locus_tag="R8G18_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HEE9795302.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="R8G18_001578"
     CDS             complement(11939..12250)
                     /locus_tag="R8G18_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEE9795303.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="R8G18_001579"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="R8G18_001579"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="R8G18_001580"
     CDS             12629..12982
                     /locus_tag="R8G18_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEE9795304.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="R8G18_001581"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="R8G18_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="R8G18_001582"
     CDS             complement(14736..15206)
                     /locus_tag="R8G18_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HEE9795305.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="R8G18_001583"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="R8G18_001583"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HEE9795306.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="R8G18_001584"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="R8G18_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HEE9795307.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="R8G18_001585"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="R8G18_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HEE9795308.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="R8G18_001586"
     CDS             complement(17208..20357)
                     /locus_tag="R8G18_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEE9795309.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="R8G18_001587"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="R8G18_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HEE9795310.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="R8G18_001588"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="R8G18_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HEE9795311.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="R8G18_001589"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="R8G18_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HEE9795312.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="R8G18_001590"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="R8G18_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HEE9795313.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="R8G18_001591"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="R8G18_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HEE9795314.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="R8G18_001592"
     CDS             26836..27213
                     /locus_tag="R8G18_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HEE9795315.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="R8G18_001593"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="R8G18_001593"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEE9795316.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="R8G18_001594"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="R8G18_001594"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEE9795317.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="R8G18_001594"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="R8G18_001595"
     CDS             30024..30353
                     /locus_tag="R8G18_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEE9795318.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="R8G18_001596"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="R8G18_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEE9795319.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="R8G18_001597"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="R8G18_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEE9795320.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="R8G18_001598"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="R8G18_001598"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEE9795321.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="R8G18_001599"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="R8G18_001599"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEE9795322.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="R8G18_001600"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="R8G18_001600"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HEE9795323.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="R8G18_001601"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="R8G18_001601"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HEE9795324.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="R8G18_001602"
     CDS             complement(37406..39082)
                     /locus_tag="R8G18_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HEE9795325.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9527 a        10050 c        10421 g         9413 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgata cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgctg g
//
LOCUS       DAQSWQ010000030        39066 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.030, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000030 DAQSWQ010000000
VERSION     DAQSWQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39066
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.030"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..906)
                     /locus_tag="R8G18_001603"
     CDS             complement(<1..906)
                     /locus_tag="R8G18_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HEE9795326.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="R8G18_001604"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="R8G18_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEE9795327.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="R8G18_001605"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="R8G18_001605"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEE9795328.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="R8G18_001606"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="R8G18_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HEE9795329.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="R8G18_001607"
     CDS             complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="R8G18_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HEE9795330.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6387..6629
                     /locus_tag="R8G18_001608"
                     /pseudo
     CDS             6387..6629
                     /locus_tag="R8G18_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6671..7747)
                     /gene="glpQ"
                     /locus_tag="R8G18_001609"
     CDS             complement(6671..7747)
                     /gene="glpQ"
                     /locus_tag="R8G18_001609"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEE9795331.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7752..9110)
                     /gene="glpT"
                     /locus_tag="R8G18_001610"
     CDS             complement(7752..9110)
                     /gene="glpT"
                     /locus_tag="R8G18_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HEE9795332.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9383..11011
                     /gene="glpA"
                     /locus_tag="R8G18_001611"
     CDS             9383..11011
                     /gene="glpA"
                     /locus_tag="R8G18_001611"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HEE9795333.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11001..12260
                     /gene="glpB"
                     /locus_tag="R8G18_001612"
     CDS             11001..12260
                     /gene="glpB"
                     /locus_tag="R8G18_001612"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HEE9795334.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            12257..13447
                     /gene="glpC"
                     /locus_tag="R8G18_001613"
     CDS             12257..13447
                     /gene="glpC"
                     /locus_tag="R8G18_001613"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HEE9795335.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            13640..14542
                     /locus_tag="R8G18_001614"
     CDS             13640..14542
                     /locus_tag="R8G18_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEE9795336.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(14583..15386)
                     /gene="yfaU"
                     /locus_tag="R8G18_001615"
     CDS             complement(14583..15386)
                     /gene="yfaU"
                     /locus_tag="R8G18_001615"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HEE9795337.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(15404..16693)
                     /locus_tag="R8G18_001616"
     CDS             complement(15404..16693)
                     /locus_tag="R8G18_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9795338.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(16750..17955)
                     /gene="rhmD"
                     /locus_tag="R8G18_001617"
     CDS             complement(16750..17955)
                     /gene="rhmD"
                     /locus_tag="R8G18_001617"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HEE9795339.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(17970..18752)
                     /locus_tag="R8G18_001618"
     CDS             complement(17970..18752)
                     /locus_tag="R8G18_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEE9795340.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(18972..20174)
                     /locus_tag="R8G18_001619"
     CDS             complement(18972..20174)
                     /locus_tag="R8G18_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HEE9795341.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(20274..20816)
                     /locus_tag="R8G18_001620"
     CDS             complement(20274..20816)
                     /locus_tag="R8G18_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795342.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            21095..21520
                     /gene="nudI"
                     /locus_tag="R8G18_001621"
     CDS             21095..21520
                     /gene="nudI"
                     /locus_tag="R8G18_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HEE9795343.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(21559..22161)
                     /locus_tag="R8G18_001622"
     CDS             complement(21559..22161)
                     /locus_tag="R8G18_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEE9795344.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            22469..23608
                     /gene="arnB"
                     /locus_tag="R8G18_001623"
     CDS             22469..23608
                     /gene="arnB"
                     /locus_tag="R8G18_001623"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HEE9795345.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            23612..24580
                     /gene="arnC"
                     /locus_tag="R8G18_001624"
     CDS             23612..24580
                     /gene="arnC"
                     /locus_tag="R8G18_001624"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HEE9795346.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            24580..26562
                     /gene="arnA"
                     /locus_tag="R8G18_001625"
     CDS             24580..26562
                     /gene="arnA"
                     /locus_tag="R8G18_001625"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HEE9795347.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            26559..27449
                     /locus_tag="R8G18_001626"
     CDS             26559..27449
                     /locus_tag="R8G18_001626"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HEE9795348.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            27449..29101
                     /gene="arnT"
                     /locus_tag="R8G18_001627"
     CDS             27449..29101
                     /gene="arnT"
                     /locus_tag="R8G18_001627"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HEE9795349.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            29098..29433
                     /gene="arnE"
                     /locus_tag="R8G18_001628"
     CDS             29098..29433
                     /gene="arnE"
                     /locus_tag="R8G18_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HEE9795350.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            29433..29819
                     /gene="arnF"
                     /locus_tag="R8G18_001629"
     CDS             29433..29819
                     /gene="arnF"
                     /locus_tag="R8G18_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HEE9795351.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(29813..30079)
                     /gene="pmrD"
                     /locus_tag="R8G18_001630"
     CDS             complement(29813..30079)
                     /gene="pmrD"
                     /locus_tag="R8G18_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HEE9795352.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(30189..31544)
                     /gene="menE"
                     /locus_tag="R8G18_001631"
     CDS             complement(30189..31544)
                     /gene="menE"
                     /locus_tag="R8G18_001631"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEE9795353.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(31541..32503)
                     /gene="menC"
                     /locus_tag="R8G18_001632"
     CDS             complement(31541..32503)
                     /gene="menC"
                     /locus_tag="R8G18_001632"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEE9795354.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(32503..33360)
                     /gene="menB"
                     /locus_tag="R8G18_001633"
     CDS             complement(32503..33360)
                     /gene="menB"
                     /locus_tag="R8G18_001633"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEE9795355.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(33375..34133)
                     /gene="menH"
                     /locus_tag="R8G18_001634"
     CDS             complement(33375..34133)
                     /gene="menH"
                     /locus_tag="R8G18_001634"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HEE9795356.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(34130..35800)
                     /gene="menD"
                     /locus_tag="R8G18_001635"
     CDS             complement(34130..35800)
                     /gene="menD"
                     /locus_tag="R8G18_001635"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEE9795357.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(35889..37184)
                     /gene="menF"
                     /locus_tag="R8G18_001636"
     CDS             complement(35889..37184)
                     /gene="menF"
                     /locus_tag="R8G18_001636"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HEE9795358.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(37263..37568)
                     /gene="elaB"
                     /locus_tag="R8G18_001637"
     CDS             complement(37263..37568)
                     /gene="elaB"
                     /locus_tag="R8G18_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HEE9795359.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(37623..38084)
                     /locus_tag="R8G18_001638"
     CDS             complement(37623..38084)
                     /locus_tag="R8G18_001638"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEE9795360.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            38149..39066
                     /gene="rbn"
                     /locus_tag="R8G18_001639"
     CDS             38149..39066
                     /gene="rbn"
                     /locus_tag="R8G18_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HEE9795361.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         9582 a        10490 c         9666 g         9328 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga cagatttcgt
     6541 tgttggctaa agaaaagccg gtagaaggca gtccactgtt tttactacaa atgtgcatgg
     6601 aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg aacgccatcc
     6661 tgcagtagca ttactcttta ttaaggaatt ttactgcctt atcagggaaa tcagtaaaca
     6721 gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca tcagtagtgt
     6781 attcaggcag tttatccgac cgcacggtat aaggatgcac taccagttta ttctgctgag
     6841 catcttgcac catgccagtg agtttgatat tacccggctg cgatgtttcc tcaatcaaca
     6901 tatggtaatc cggcccgata ccgtcggcat attccgcgac ttgcttcatg gcacctggct
     6961 taaacatcca gtcatagctg taattaaccc agcttccgtc cggctgtttc tgctgcgttt
     7021 cattccagtc ggtataggca atcagctgta ccagattgag atccatgccc attttaggtt
     7081 ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa taaactttat
     7141 cgtccttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc gcaatatcct
     7201 tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg atatttttcc
     7261 cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg tgcccccgga
     7321 agtcggactt acccattggg aaacgccccg gataagtctg tactttttta ccgttttcaa
     7381 tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa tctatcgcgt
     7441 agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca gtaacacgat
     7501 cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc aaatcctgtt
     7561 ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta tgctccggca
     7621 aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc gccgccattg
     7681 cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt ttcagcttca
     7741 atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg acgtttttcg
     7801 ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat cattaccata
     7861 aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct cgccgccacc
     7921 gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc cgcttttttc
     7981 ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat caggaagccg
     8041 ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat ccagtaaacg
     8101 atagtcgcga tggtcaccag tgtcataaag aaaacgccgg ttgccccacg gttgccacgg
     8161 aagactttat ccgacatcca gccgcacagc agagtgcccg gaatacctgc atattcataa
     8221 aggaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt cagataagtc
     8281 ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc gatggcgata
     8341 taccacagca gtttgttcgg cagtacgtac tgcatgaaga tttgcttcgc cgtcagctcc
     8401 tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc gatcggcggc
     8461 aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc caccagaatg
     8521 gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc catccccagc
     8581 aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca cactgacaca
     8641 atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca cggcggccac
     8701 cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc aatgctcgac
     8761 gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat caaacctgcg
     8821 ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa tttcgaaaat
     8881 ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga gaatccctgc
     8941 tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta agccgcatag
     9001 ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg atcgatctcc
     9061 gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat tgatagcctc
     9121 cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac ctgccgtttc
     9181 ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc cgcacacatt
     9241 attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc actttcgaat
     9301 catgagcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta aatggtaaaa
     9361 aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg acgtgattat
     9421 cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc gcgggctgcg
     9481 cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta accacggcct
     9541 gctacacagc ggtgcgcgct atgcggtaac cgatgcggaa tcggcccgcg aatgcattag
     9601 tgaaaaccag atcctgaaac gcattgcacg tcactgcgtt gaaccaacca acggcctgtt
     9661 tattaccctg ccggaagatg acctctcctt ccaggccact tttattcgcg cctgcgaaga
     9721 agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg aacctgccgt
     9781 taacccggca ttgattggcg cggtgaaagt tccggatggc accgttgatc catttcgtct
     9841 gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta ccgctcatga
     9901 agtcacggga ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac gtaaccatct
     9961 caccggcgaa actcaggccc ttcatgcgcc tgtcgtggtt aatgccgccg ggatctgggg
    10021 gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga aaggatcgct
    10081 gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta aaccttccga
    10141 cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggcaccacct ctttacgtat
    10201 tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata ttctgctgcg
    10261 tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg cctattctgg
    10321 cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca gccgtggcat
    10381 cgtgctgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca tcaccggtgg
    10441 caaactgatg acctatcggc tgatggctga atgggctacc gacgccgtgt gccgcaaact
    10501 gggcaacacg cgcccctgta cgaccgccga tctggcactt cctggttcac aggaacccgc
    10561 tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt ctgcggttta
    10621 tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggt cgtctgcacc gtagcctggt
    10681 atgtgagtgc gaagcggtaa cagcgggtga agtgcagtac gcggtagaaa atttaaacgt
    10741 taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggtacct gccagggcga
    10801 actctgcgcc tgccgcgctg ccggactgct gcaacgtttt aacgtcacga cgtccgcgca
    10861 atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc aacccatcgc
    10921 ctggggagat gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg gactgtgtgg
    10981 tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcggc ggcctcgccg
    11041 gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt gtcacgcgtg
    11101 gtcaaagcgc gctgcatttc tcatccggtt cgctggattt gctgagccat ctgcctgatg
    11161 gtcaaccggt gacagacatt cacagtggac tggaatcttt gcgtcagcag gcaccagccc
    11221 atccttacag tcttctcgaa ccacaacgcg tgctcgatct cgcttgccag gcgcaggcat
    11281 tgatcgctga aagcggtgcg caattgcagg gcagcgtgga actggcccat cagcgcgtta
    11341 ccccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc gtctggccgc
    11401 tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc caggcgcatc
    11461 ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa atagagctgc
    11521 cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat atcgcccgtt
    11581 tccttgataa tgaagaaaac tggccgctgt tacttgatgc gcttattcct gtcgccaata
    11641 cctgcgaaat gatcctgatg cccgcctgct tcggcctggc cgatgacaaa ctgtggcgtt
    11701 ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg ccttccgtgc
    11761 tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt ggcgtgtgga
    11821 tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat gaaatctgga
    11881 cccgcaatca cgccgatatt ccgctacgtc cacgtttcgc ggttctcgcc agcggcagtt
    11941 tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt ctcggccttg
    12001 atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt gcgccgcaac
    12061 cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag gcagggcaaa
    12121 ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc atcgcccagg
    12181 gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag attgcccaac
    12241 gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt gcaccgtctg
    12301 caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa aacaagccgg
    12361 gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg cgctgaaata
    12421 ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga ttggcgatat
    12481 tatccagcgc gcgcgggcaa aatatgacac cacgcgcccg tcgctgcgta attttgtgtt
    12541 gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg tcaacaccgc
    12601 tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg atcatcgtcg
    12661 cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca tagcggctca
    12721 gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta actacaacca
    12781 tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtatcg gtgtacaact
    12841 gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta ccgataaagc
    12901 acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag taaaaggcat
    12961 tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat acccggaagt
    13021 gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc gctggctgtg
    13081 gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga aagtggttta
    13141 tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg agctgttgcg
    13201 taaaatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta ttgcgggtac
    13261 ttacggtttc aaaaaagaga actaccccac ctcacaagcc atcggcgcac cactgttccg
    13321 ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct gtaaatggca
    13381 gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg cccaggcgct
    13441 ggcttaaact cctttctgat gcccggtaag catgtggtta ccgggcattt ttgcgtacac
    13501 gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa acgtctcgca
    13561 aaggctgaaa ctggcagatg tcaaaggcct gggataaccg taatgtcgcg tcatcataaa
    13621 tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg atgcggtatt
    13681 taaaaccttt atgttcacac ccgaaaccgc acgggatttt ctcgaaatac atttgccaga
    13741 accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta gttttattga
    13801 aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca gcgacggtga
    13861 cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata tggcttttcg
    13921 gctaatgcgc tatgccactg ccgccatgca gcgtcacctg gataaaggct atgacagagt
    13981 tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc cgtactcact
    14041 taactggctg gatgagtttg acgatccgca acttgcccgg cagttgtaca ccgaagcttt
    14101 tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc ggcgtatagc
    14161 tctgcttgaa ctgattcaaa agcatattcg cgaccgcgat ttaatcggta tggtcgacag
    14221 gatcaccacg cttttggtta aaggcttcac taatgacagc cagctacaaa cactgtttaa
    14281 ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattgagg agatttccga
    14341 acgttcacca ctacaaaagg agagattgat gactattgct gagcggctac ggcaggaagg
    14401 acaccaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg ctttgcgcat
    14461 gctggaacag ggcattgatc gtgaccaggt gctcgcggcc acccagctaa gcgaagccga
    14521 tctggcagcg aataaccact aattaacaca ggcccacagc cgatccccat gggcctttga
    14581 tatcaataac taccttttat gcgtggccca tttttgcctg atttaaacat cgccagtcgt
    14641 tgatccaggg catcgctgta gagcatcgtg tcaacgccaa cagcgacaaa gttcgctccc
    14701 cacgccaggc attgctgcgc catatcagga gccacagcca gaaaaccagc cgctttaccc
    14761 gcagcacgga tccgccgaat gctggtttca ataattcgct gcacttccgg gtgcccggcg
    14821 ttatccgggt agcccaacga cgcagaaaga tccgctggtc caataaacac gccatcaatc
    14881 ccttcgacgt cgaggatctc gtccaggtta tccagtgccg ttttactttc cacctgcact
    14941 aacagacaaa gcgaatcgtt gacttgcgcc atgtaattct caatgcgtcc ccagcgcgca
    15001 gcccgtgcca cactggcccc aacaccacgc tcaccgtagg gaggatagcg cgtggcagac
    15061 gccacctgac gagcctgatc ggcagtatcg accatcggga tcaatagagt ttgcgcgcca
    15121 atatccagaa cttgtttaat cagcgattta ctgccttcca ccggacggat cacgggttgg
    15181 ctggcatagg gcgctaccgc ctgtagctgg tgataaagat cctgaatggt gtttggcgcg
    15241 tgctccccgt caatcagcaa ccagtcataa ccggaagtag cggcaatttc tgccatataa
    15301 gaagtagtcg agcttaacca cagaccaatt tgcacttcgc ccttgcgtaa acgttcttta
    15361 aagggattgg ttaataatgc gttcatcgtg ttccttaacc tatttaatga tgtgccacgt
    15421 cggtctgcgc aacagtacga tttacacgca gagtgaaaat aatcagcgaa ccgatgaccg
    15481 ccaccgctgc cagtgtcagt aatcctgccg catcgctggc aaataacgtt tctgctttca
    15541 cgcgcaggat cggggcaata aacccgccca ctgcaccaaa caggtttaca aaaccaatcc
    15601 ccgcagccag cgccgtaccg gaaagcagtt gtgtcggcat cgtccagaac accggctgca
    15661 cggcaataaa cccgatagct gcaacacaca gcgctacgat cgccattact ggagaaagca
    15721 gcccggacag accaatacca atgcccgccg ccagtaatgt cagcgctgcg acattacgcc
    15781 gttcgccggt tttatcggaa tagcgcggaa taagccaggt cccaaacaag gccgcaaccc
    15841 acggaatggc ggtgaccacc gacgctgtaa agcccacttt tgttcccagc aatgccgcaa
    15901 cctgggtcgg caggaagaaa attaatccgt aaaccgccac ctgaatggtc aggtagataa
    15961 tcgctagttg ccagactcgg ccattacgca gcgcatcgct cagccgagaa gtcactttct
    16021 gttgttcttc acttgccagt tgattgataa gcaacgtttt ttcttgttta ctcagaaaac
    16081 gtgcctgctc cggtgtgtca tcaagccaaa agaatgtgaa taccccagca ccgactgcca
    16141 acaatccttc aatcacaaac atccagaacc agccgggatg ccccataaat ccatgcatct
    16201 ccaacagcgc gccggaaagc ggtgatccca gtgttaacgc cagcggtgcg cccatataga
    16261 acagccccat aatgctggcg cgattacgct gcggaaacca ttgcgaggtg agataaatca
    16321 taccagggaa aaatccggcc tccgcagcac caagcagagt gcgaactatc agaaatttcg
    16381 cttcagtatc ggcccatgcc atggctgccg aaagaaatcc ccacagcagt gttgtcgtac
    16441 caatccaggt tctggccccc agtttgcgca tcaaaagatt cgccggaaca cccagaaacg
    16501 catataccac aaagaaaatg cctgctccca gcgcataagc ttcattactc aacccggtat
    16561 caatctggta ggtctgtttg gcaaaaccga tattcgaacg gtcgagaaac gccagcacat
    16621 acagcgccaa cataaacgga attaaacgca cacggttttt cttcaccacg gcgtcaagca
    16681 aagcggtgct cataaaaagc tccttagaat atgtgggcgt taccgttgtc acagcaacgc
    16741 ccggagtgat tagtggctgt aggggcgttt caaattgcag tcacgattga gttcgacgcc
    16801 aaaaccgggt ttatcaagca ctgatttatg aatacgacca ttcaccggaa ccggctcatt
    16861 gagcagaatc gggtcaaact gcggacgcat cgttgaacaa tccgggctgg tcatcaggaa
    16921 ttcgctgaat ggcgtattgg tgaaggtgat caccgcatgg tgagagtaaa cagacgaacc
    16981 gtgcggcacc accagttgcc ccctggattt ggcgattgcg gcaatttcca ccagcgtggt
    17041 taaaccaccg caccagccaa catccggctg cataatgtcg ataccggttt ctgaaagcgt
    17101 acgaaaagat tgcagtgtgc cgtggtgctc accgctggtg accatcattc cgactggcgc
    17161 gttgcgtttc agttcgcgat aaccttcata ctgctgtggc ggcaggcact cttcgatcca
    17221 tttcaggtta tagggcgcgc aagcgtgggc cagtttggtg gcatagttca cgtcctgact
    17281 catccagcag tcgagcatta accagaaatc ctcaccgcat ttttcacgca tatccgcgac
    17341 cataacggca tctttgcgga tccccgcatc gccatcatgt ggcccccagt gcgtcggcat
    17401 tttgccaccg ataaagccca tctcttttgc cagatccgga cgcgcgcctg tggcgtagaa
    17461 ctgaatctca tcacgaactg cgccgcctaa aagtttataa accggaagcc cgaccacttt
    17521 gccgaacaga tcccacagag ccagatcgac acaggaaatc gtgttcatca ccaggccacc
    17581 agagccggag taatacaggg tggcattgag catttgatca tggatcagtt tgatatcact
    17641 gacacattta ccctcaatga aacggttaag atgtttttcg acaataaaac agcccatttc
    17701 accggctgtc gaaacagcga atccggtctg gccgttttct gcttcgactt caacaatcaa
    17761 cgtgccaaga acgttaatgc caaatgactg gcgtgactgc tcgtaatcgc ggtatttact
    17821 catcggggtg gcaatatgat cgtcaatcca gtgattcgcc ccctggtcgt gataatcacc
    17881 gccgccagca cctttttctg ctgtcgcacc gccagtaaac caggcgcgaa cctgtttgat
    17941 ttttggtagg gtcatgatgt tctccattgt tatgaggcct gtaagtcaaa gggacttttc
    18001 catcccaaca gacgtgaaat atccctggcg caggcaatgg ccttgcccgc cagataatca
    18061 cggtattctt cattgatttg taagcgggta ccgaccaccg agatcgcagc ggtaagctcg
    18121 ttattggcgt taaacaccgg cgcagcgaca caacggacat cggggtaatc ttcgccgttg
    18181 tcatagctcc agccctgacg gcgaatacgc gccagttctt cgtgaagttg ctgtggatga
    18241 gtaatcgttg tgggtgtcgc ctgctcccag accagccctt cgataatact ttgctgtacc
    18301 gctgcaggtt gccaggcaag caggcattta cctattccgg agcgataaag cgaaaggctt
    18361 tttccttcat gggaacgcac gctgatagtg gctgatgact ccacttttaa aatgtaataa
    18421 gcgctgccgt tatcaataat ccccaggtga cataacagcc cggtggtatc catcagttgc
    18481 gtcaaacgcg gtcgagccag ttcccggaga tccatcttgc ttaacgcatg accagaaagc
    18541 tccaccagcc tggtccacaa acaaaaattt tcctggtgat caaggctaag aaaacgctga
    18601 cgcctgagtt cattaagcag caaataggcc gtgcttttag ggattcccaa tgtgtcaatg
    18661 atcgtcgcag cactacaggg gccaatgcgg gcaatcaaat tgagaatatc aatagcccgt
    18721 gtcagggcgg gaactttgct tgattccaac atactggact ccagtcttag atactggaat
    18781 cagtctcgtt gctcaggggg agtaaaattg tgaagcgcat caaatcccgc tcctcgtcca
    18841 gcggactgga gaaattacgc acaaatttgt gaatcagtgc acggattttt tcttattgag
    18901 tacaacaggt tggacaagtt ttgccggagg gggaatgtgc cggtgcagta aaccggcaca
    18961 gagtggcgga atcagacaga taaggtcatg gactcaacaa cctcaatcca gccatgctca
    19021 ctggcgatat cctggccgtt taaccaacgg cgcagcatat tcagtgccat cattgcgcac
    19081 acttcctgac ggatagccag gctgtagcgt gtagtgctga aacgcacacg cagagcgaaa
    19141 gtgccgtctg gcgtggctag cgcaaagttg agatgctcgt tctcgaaacc cgaaacagcc
    19201 agtgccagcc cggcaaaatg gttggcccgc cgttctgtaa tccagtgcgc agtttgcgcc
    19261 agggtttctt cctgtgaagg aaccacttca cacgccagca atggagcacc tgcgcgagaa
    19321 agttgcaaag ccaataaacc accggtgaat tgctcgctca acgtcaggct gaactggcgg
    19381 ttttgcaatt cgcgactgat ctgcgcgggc agtccttcgg tgccttcgaa aatcacgctc
    19441 tgtccggcaa cacgtttaac gtccagccac agtttttcca tcgcctgttg ctcgcttgcc
    19501 ggtccggtga gtttcagttc gatgatcggc attgaggagc gatagcccat tgttacaccc
    19561 ggcggcagtt gtagagtgtc caggctttgt gccagatcgc tttccgaacg accaaaggta
    19621 gttaaacgca gacaaaccgg cgactgtggt aaagaaaaac gctcgcgcag gcgcggcagg
    19681 atttcgtgct cgaccatcac cttaaattct gacggtacgc cgggagtaaa gaacatcagg
    19741 caacgattaa gccgcacggc aaaaccacag gcggtgccta ccgggttatt gataaattca
    19801 gcactggcag gcagctccgc ttgtttacgg ttgctcggtg ccattactcg tccacgttcg
    19861 tggaaatagc gttccatctc tttgagccag gcttcatgca gcaccaggcc ttcacctttt
    19921 gctgtcgcag cggcgagtgc acttaaatca tcactggtcg gtcccagccc gccgttaacg
    19981 atcagcacat cggcgtgctg actacgttcg cgaagaatgg tgactaagtc atcaaggtta
    20041 tcccccaccg tattgcggcg agataatggc aacccctgat gaaagaaaaa atcggccagc
    20101 caagcagcgt tagtgtcaac gatttgcccg tgtaacactt catccccggt ggataacatt
    20161 tccactttta acatggttat ctcctgcttt tgttatcgaa tcactataac gcaaagggag
    20221 ataaggcgta gtaagaaact gaatggcgcg acagaatgcc gcgccggaag ggatcagaaa
    20281 ctggcgttaa ccccaacata cgggccgtca gccacggcgt tgtcgcggtt accgtcttta
    20341 cccgacagat tcaggtagcg ataacccgcc tcaatactga ctggatgcat aatggtgtaa
    20401 cgcgcgccag cattcgcttc ctcataactt ttaataccgc tggagagtga atccggagag
    20461 tagtaatact cgccaaacaa acggaagctg ttgccaattt tccactgcaa accacctcct
    20521 accgctgcgg cataaccttc atcgccgtaa ttcgggttgg tgtaaacgcc ttttccgcca
    20581 acggtcgcca ttaacggccc gagaggcaga ttcaacccca gccccacgcc agcgacgtca
    20641 ccgtcgtcgt cgttatgtgt ccagttaccg cttaacgcca ggcccgtcga ttcagtccca
    20701 aaaccgacac caatattggt gtattcttta cccgcctgac cgctgatgct cattgcattg
    20761 accgatgcag aaaccaacag cattccggcc agacctgcta gcgcaatttt tttcattatc
    20821 ccctttccca caagcaatta atcaaaattc ccaaagctgc gggattgtac tgcctggcac
    20881 agaggattca atgagcgaaa aaagggatct agtcacttat tgtaactaaa tacttcttaa
    20941 ataattaaac gccgaaaaaa taaacacact ttaccgatac acacaaaaat aataataagt
    21001 gcagttatca tcgcaaaaga gattactgtc actttcacat atttctgagt taccgtattc
    21061 tcatggtttc tttttattta taaggaatac ccctgtgcga caacggacta ttgtatgccc
    21121 tttgattcaa aatgatggtg cttatttgct gtgcaaaatg gccgacgatc gcggcgtttt
    21181 ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct ggcgaacgaa ttgaagaggc
    21241 actacgccgc gaaattcgcg aagaactggg agaacagctg cttttgacag aaatcacgcc
    21301 gtggaccttc agcgatgata ttcgcaccaa gacgtatgca gatggtcgca aggaagagat
    21361 ttatatgatt tacctgattt ttgactgcgt ttctgccaac cgagaagtga aaataaacga
    21421 agagtttcag gactacgcgt gggtaaaacc tgaagatctg gtgcattatg atttaaatgt
    21481 tgccacccga aaaacgttac gtttgaaagg tcttctttaa caatgatgac agccatcact
    21541 ggctgtcaga ttgaaaattt agtggttaac gaattcccca tccagataaa ctttgccttt
    21601 ttccacatgc atgactaaac catccagata atcaggttta aatgtcgcat cacgcttatt
    21661 tttagcaata tatgtcaggc aatgattatg ggtgaaaata acgatatttt tatcaggcgc
    21721 tttgctttgt aagtccttaa ttgcactata aatctcatta ccgcactgaa gaagtcgttt
    21781 atctaccgtc agttttttac ccgcggaaaa ccaggtcgcc gactgaatgg tccggacggt
    21841 attactggaa taaagatcga aatcagggat gtcagcgcta aaagcgttgc ccagttcacg
    21901 ggcatcctgg gtacctttaa ccgtaatacc tgttttatct gacaagcatt gattggttga
    21961 acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc gggtgctgct gtgccagtct
    22021 ggccaatgtt ttattgtcga tacgtggcaa accattgcta ctccaggcgg catgagtacc
    22081 cagtccggca attgcagcga gcgccagtaa aatgatgaaa tatttttttg acttcaatga
    22141 tgagcggcaa aaagctaaca cgtaaactcc acctatagac aagcgcaacc agacaattac
    22201 cgtgaaattg agcgacattt ctggcgataa ttagcagttg gtgtaatatt aaaaatccta
    22261 cgatgtcggc aaaatgcctc aaaattttgc caaatgcaaa gcctaaataa gaaaaaatat
    22321 aaaaatttca atatttacgt ctaatattag tttcttaagg ttaagttaat attctatcct
    22381 taaaatttcg ctccaaatgg caaaatatac acaacactct ttatagcaaa tataagtgga
    22441 caggtattca atggcggaag gaaaagcaat gtcagaattt ttgccttttt cgcgaccagc
    22501 aatgggcgtg gaggaactcg ctgcagttaa agaggttctc gaatccggtt ggatcacaac
    22561 cggtccgaag aatcaggcgc ttgagcaagc cttttgccag ttgacgggaa atcagcatgc
    22621 catcgcggtc agttcagcca ccgccggaat gcatatcacg ctaatggcgt tggaaattgg
    22681 caagggcgat gaagtgatta cgccttccct gacctgggtt tcaaccctca atatgatttc
    22741 cttgttgggt gcaacgccgg taatggtgga tgtcgaccgc gatacgctga tggtcacgcc
    22801 tgaagcgatc gagtcagcca ttacgccacg cactaaagcc atcattccgg tgcattatgc
    22861 cggtgcgcca gcagatattg acgccattcg cgccattggc gaacgttacg gcatcgcagt
    22921 tatcgaagat gctgcccatg ccgtcggtac gtattacaaa gggcgacata ttggcgcaaa
    22981 aggtaccgct attttttcat ttcatgccat taaaaatatt acctgtgcgg aaggtggcct
    23041 gattgtaact gataatgaaa accttgcccg ccagctacgg atgctgaaat ttcacggtct
    23101 gggtgtcgat gcctatgaca gacaaacctg gggccgtgca ccgcaggctg aagtcttaac
    23161 accgggctat aagtacaatc tgaccgatat taacgccgcg attgccctga cacagttagc
    23221 aaaattagag cacctcaaca cccgtcggcg cgaaattgcc cagcaatatc agcaagcact
    23281 ggcagctctc ccctttcagc cattaagcct tcccgcctgg ccgcacgttc acgcctggca
    23341 tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc agtcgcgatg cgttgatgga
    23401 agcgttaaaa gaaagaggca ttggtaccgg gttacatttc cgcgccgctc acacacaaaa
    23461 atattatcgc gagcgttttc ccacgctgtc gttaccgaat accgaatgga atagcgaacg
    23521 catctgttct ttgccgctgt tcccggatat gactaccgcc gatgccgacc gtgtcatcac
    23581 agcccttcag caactcgcag gacaataagc catgtttgaa atccaccctg ttaagaaagt
    23641 ctcggtggtt attcccgttt ataacgagca ggaaagctta ccggaattaa tcaggcgcac
    23701 caccacagcc tgtgaatcgt tggggaaaga gtatgagatc ctgctgattg atgacggcag
    23761 tagcgataat tccgcgcata tgctggtcga agcctcacaa gcggagaaca gccatattgt
    23821 gtctattttg cttaaccgca attacgggca acattcagcg attatggcgg gattcagtca
    23881 cgttactggc gacttaatta ttacccttga tgccgatctc cagaatccgc cagaagaaat
    23941 cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg gtagggactg tacgccagaa
    24001 ccgccaggac agctggtttc gtaaaacagc ttcgaagatg attaacaggc ttattcagcg
    24061 caccaccggc aaagcgatgg gtgactacgg ttgtatgctg cgcgcctatc gccgtcatat
    24121 tgtcgatgcg atgttgcact gccatgaacg tagcaccttt atcccgattc tggcgaatat
    24181 cttcgcccgc cgtgccattg aaattccagt acatcatgcc gagcgtgagt ttggtgaatc
    24241 caaatacagt tttatgcgcc tgattaattt gatgtacgac ctggtgacct gccttaccac
    24301 aacgccgcta cgtatgctga gtctgctcgg cagcattatt gcgattggag gttttagcat
    24361 tgcggtgctg ctggtgattt tacgcctgac cttcggacca caatgggcgg cagaaggcgt
    24421 ctttatgcta tttgccgtgc tgtttacttt tattggcgct cagtttatcg gcatgggatt
    24481 gctcggtgaa tatatcggca ggatctacac cgatgtccgc gcccgccccc gctattttgt
    24541 tcagcaagtt atccgtccat ccagcaagga aaatgaataa tgaaaaccgt cgtttttgcc
    24601 taccacgata tgggatgcct cggtattgaa gctctgctgg ctgccggtta cgaaattagc
    24661 gccattttta cccataccga taatcccgct gaaaaagcct tttatggttc ggtggctcgt
    24721 ctggcggcgg aaagaggcat tccagtttat gcgccggata acgttaatca tccgctgtgg
    24781 gtggaacgca ttgcccaact ctcaccagag gtgattttct ctttttatta tcgccatctt
    24841 atttgcgacg agattttgca gctcgctccc gcaggtgcat ttaatctgca cggttcgctg
    24901 ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc tggtgaacgg tgaaacggaa
    24961 acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg ctggggccat tgtggcgcaa
    25021 ctgcgcgttg ccattgcgcc agacgatatc gctattacgc tgcatcataa attgtgccat
    25081 gccgcgcgcc agctactgga acagacatta cccgccatta aacacggtaa tattctggaa
    25141 attgcccagc gcgaaaacga agccacctgt tttggtcaca gaacgccaga tgacagtttc
    25201 cttgaatggc ataaaccggc atccgtactg cacaacatgg tacgtgccgt tgccgatccg
    25261 tggccgggtg ccttcagcta tgttggcaat cagaaattca ccgtctggtc gtcgcgtgtt
    25321 catcctcatg ccagcaaagc acagccgggg agcgtgattt ctgttgcgcc actgctgatt
    25381 gcctgtggcg atggcgcgct ggaaatcgtc accggacagg cgggcgacgg cattactatg
    25441 cagggctcgc aattagcgca gatgctgggc ctggtgcaag gttcacgctt gaatagccag
    25501 cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc tcggggtgaa tggctttatt
    25561 ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt atgaagttta cggtctggat
    25621 attggcagcg atgcgataag ccgttttctg aatcatccgc attttcactt tgtcgaaggc
    25681 gatatcagta ttcattccga atggattgag tatcacgtca aaaaatgtga tgtcgtcttg
    25741 ccgctggtgg cgatagccac gccgattgaa tatacccgca acccgctgcg cgtatttgaa
    25801 ctcgattttg aagagaatct gcgcattatc cgctactgcg ttaagtaccg taagcgaatc
    25861 atcttcccgt caacttcaga agtttatggg atgtgtagcg ataaatactt cgatgaggac
    25921 cattctaatt taatcgtcgg cccggtgaat aaaccacgct ggatttattc ggtgtcaaaa
    25981 caattacttg atcgggtgat ctgggcctat ggcgaaaaag agggtttaca gttcaccctc
    26041 ttccgcccgt ttaactggat gggaccacga ctggataacc ttaatgcagc gcgaattggc
    26101 agctcccgcg ctattacgca actcattctc aatctggtag aaggttcacc aattaagcta
    26161 attgatggcg gaaaacaaaa acgctgcttt actgatatcc gggacggtat cgaggcgttg
    26221 taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg aaattatcaa cattggcaat
    26281 cctgagaacg aagcgagcat tgaggaactg ggcgagatgc tgctggcgag cttcgaaaaa
    26341 catccgctgc gccatcactt cccaccgttt gcgggctttc gtgttgtcga aagtagcagc
    26401 tactacggca aaggatatca ggacgtagag catcgtaaac cgagcatccg caatgcccgc
    26461 cgctgcctgg actgggaacc gaaaattgat atgcaggaaa ccatcgacga aacgctggat
    26521 ttcttcctgc gcaccgttga tcttacggat aaaccatcat gaccaaagtc ggcttacgca
    26581 ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc acgtctgctg gaaatcttga
    26641 gtaagcataa tattcaggcc agcatttttt tcagcgtcgg cccggacaat atgggccgcc
    26701 atctctggcg actggtgaag ccacagtttt tgtggaagat gctgcgctca aacgcggcat
    26761 cgctttatgg ctgggatatt ttactggcag gtacggcctg gccaggtaaa gagattggtc
    26821 atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca cgaagtcggc ctgcacgcct
    26881 gggatcacca tgcctggcaa gcccgtagcg gtaactggga tcggcaaaca atgatcgacg
    26941 atattgcacg cggtcttcgc actctggaag agattatcgg tcaaccggta acctgttctg
    27001 ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc aaaagaagcg ttccatttgc
    27061 gctacaacag cgattgtcgt ggggccatgc cgttccgtcc actgctcgaa tcaggaaacc
    27121 ctggcactgc gcaaattccg gtgaccttac ccacctggga tgaagtgatt ggtcgggatg
    27181 tgaaagcaga agattttaac ggttggttac tcaaccgcat cctgcgagat aaaggcacgc
    27241 cggtttatac cattcatgca gaagttgaag gctgcgctta tcagcataat tttgtggatc
    27301 tcctcaaacg cgcagctcag gaaggcgtga cattttgccc tttaagcgaa ctgttatcag
    27361 agacgttgcc gctcggacaa gttgttcgcg gaaatattgc cggacgtgaa ggctggctgg
    27421 gttgccaaca aattgcgggt agtcgctgat gaaatcggta cgttacctta tcggcctctt
    27481 cgcgtttatt gcctgctatt acctgttacc gatcagcacg cgtctgctct ggcaacccga
    27541 tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca tccggcgact ggattgtgcc
    27601 ccatctgtta gggctacgtt atttcgaaaa acccattgcc ggatactgga ttaacagcat
    27661 tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg gcaggcgtta tctttgcgac
    27721 cctgttaact gccgcgctgg tgacctggtt tactctgcgc ttatggcgcg ataaacgtct
    27781 ggctctactc gccacagtaa tttatctctc attgtttatt gtctatgcca tcggcactta
    27841 tgccgtgctc gatccgttta ttgcattctg gctggtggcg ggaatgtgca gcttctggct
    27901 ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga tttttactgc tgggaatcac
    27961 ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt gccgtgccgg tattaagcgt
    28021 gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc tttatttacg gctggctggc
    28081 ggttatcagt tgcgtactga cggttctccc ctggggactg gcgatagcgc agcgggagcc
    28141 tgacttctgg cactattttt tctgggttga gcatattcaa cgctttgcac tggatgatgc
    28201 ccaacataga gctccgttct ggtactacgt gccggtcatc attgccggta gcctgccgtg
    28261 gctgggatta ctccccggtg cactgtacac aggctggaaa aaccgcaagc attccgcaac
    28321 cgtctatttg ttgagctgga cgataatgcc gctgctgttt ttctccgtcg ctaaaggtaa
    28381 attgcccacc tatattcttt cctgctttgc atctctggca atgctgatgg cgcattacgc
    28441 tttgctggca gcaaaaaata atcctctggc gctgcggatt aatggctgga ttaacatcgc
    28501 ttttggcttc actggcatta ttgccacatt tgtggtctcc ccgtggggac caatgaacac
    28561 gccggtgtgg caaaccttcg agagctataa agtcttttgt gcctggtcga ttttttcgct
    28621 atgggcattt ttcggctggt acaccttaac aaacgtcgaa aagacctggt cttttgccgc
    28681 gctttgcccg ctggggctgg cgttgctggt aggattttca attcctgaca gagtcatgga
    28741 aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa tcacttcagc caagccgcta
    28801 tattcttact gatagcgtcg gtgttgccgc aggtctggca tggagcctgc aacgcgatga
    28861 catcatcatg tatcgccaga caggtgagtt gaaatacggc cttaattatc cggatgcgaa
    28921 agggagattt gtcagcggtg atgagttcgc aaactggctt aatcaacatc gtcaggaggg
    28981 gattattaca ctcgtgcttt cggttgaccg cgatgaagat atcaacagtc tcgccattcc
    29041 gcccgcagat gccatcgatc gtcaggagcg tctggtgctg attcagtatc gtcccaaatg
    29101 atctggctaa cattagtctt tgccagcttg cttagcgttg ccgggcagtt gtgtcagaaa
    29161 caggcaacct gctttgtggc gataaacaaa cggcgcaaac atatcgtgct gtggctggga
    29221 ctggcgctgg cttgtcttgg tcttgccatg gtgctctggc tgctggtctt gcagaacgta
    29281 ccggtaggca ttgcctaccc gatgttaagt ctgaattttg tctgggtgac gctggctgca
    29341 gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt gtggggtggc gttcattatt
    29401 ggcggcattg tgatcctcgg gagtacggtg taatgggcct gatgtgggga ttattcagcg
    29461 tcattattgc ctcagttgcg caattaagcc tgggttttgc ggcgagtcat ctgccgccga
    29521 tgacgcactt atgggatttt atcgcggctc tgttagcttt tggcctggat gcccggatac
    29581 tgttactcgg gctgctgggt tatctgcttt ccgttttttg ttggtataaa acgctacata
    29641 aacttgcgct aagcaaagcc tatgccttgc tcagtatgag ttatgtgctg gtatggattg
    29701 cttcaatggt acttcccggc tgggaaggga ctttttcgct gaaagcacta ctgggagtag
    29761 cctgtattat gagcgggttg atgctgattt tcctgcccac gacaaaacaa cgttactgag
    29821 ttttccctgc cactttacac tgccgttccc actcatcggg gctataactg cttgccgaga
    29881 ggactttcac cgtatgcagc ttttcacgat taatacaata aagcgcattc tgcaaaggcg
    29941 agagtaaatc ccccactttc acggcgaagt cactcttcac ttcggcgatc atttttatcg
    30001 cgccgccagc atcgcagagc ataagtacat gtctgttatc ttgtttattg cagcacgatt
    30061 ttttgaccag ccattccatt gcattatcct gtttgctaag agttttcaca tcaatttaca
    30121 gattaacaga taaacctatt atcaacggca atggaatggc aaaattgcga agtgaaacta
    30181 atgatttctt aatcctggcg ttgcacccac tcttttaacg cctgacgtga aattttaata
    30241 ccgccgtttt tcagctccgg cggtaaagtt agccagcgca ccggttgttg aaaacgagcc
    30301 agcttatctt tcacccattc gccaagatca acggtttgct ggtcatactc caccaccgct
    30361 accggtcgat gaccaaactc cttgtcggca acggggacga taaacacctg caaaaccgca
    30421 ggatgtgcag caattacgcg ctcgacttct tccggctgaa taccctctcc gccactgaag
    30481 aacagattgt ctaaacgtcc gacaatggtc agcttgccat tatgcatctc accgcgatcg
    30541 cgcgtagcgt accagccttc gtcattaacc agtgaaacca gttgcccgtt acgccagtaa
    30601 ccttctgcca tactggcagc ccgcagccac acttcattat taacgatttt cacttcccga
    30661 cccggcagcg gcgaaccaac gtctgccagg ccgtcggctt ctttcgcaca caccgtggag
    30721 gcaaactcgg tcagaccata gccgcaaaag caacgaatcc cctgctcgcg cgcctgttcc
    30781 gtcaactcga ccgggatagc cgcgccgcca agtaacaccg ctttcaggga aacggaactg
    30841 cggttaacca gcaaacgcca gagttgtgtt ggcaccagtg aagcgtgagt acagcctgcc
    30901 agcatttgct ccaatggctg tttatcacgc accgtcatcc gcgcaccagc gtataaccag
    30961 cgccacataa ttccctgacc ggagacgtga aacagcggta aagagagcaa ccagtcatcg
    31021 tgatcgccaa acggaatcag cgataacaca ccttgcgcac tggcaagatg ggcctgataa
    31081 gtatggacag cggctttcgg caaaccggta gaacccgagg tcaacgtcat tgagcacaga
    31141 cgcgtcggct gccacgtagc ggcatgtgcg ccttcaaccc gctgaatgtg cagcgacgtt
    31201 aatgccggaa acgtgttttc cccatccggc accagcgcaa attgcagcgt cagattgggc
    31261 agcaattctt caagcaacgg ttgcggcagc tgagggttca cgggcaacac ccgcgccccg
    31321 cattgcagta acgccagcca ggcgagcagc gtttgcggcg tattccacgc ccgcaacatc
    31381 acgccgctgc cctcaaccac accctgcgcc gcaaagcccg tagctaactc atcgacgcga
    31441 gcacaaagct cgcgccagtt gagttgctcg tcattaagac gtaaggcgat ggcttctccc
    31501 cgcacttgcc gccagtgacg ccacggccag tcagagaaga tcataacaac tgctccagta
    31561 catcagcgtc cactaacggc agtgggctcc ccggccagcg acgaacctgc tgcgcctgca
    31621 tcagatccag cgtgtccagt cccggaatgg tgtctggcgt taaccaggca gcaatccgcg
    31681 ccagttgcgt tagacctaag ctcgactcaa tggaagaact gatcaccgcc gtcagcccca
    31741 gcgcatgcgc cgcctgtacc tgctcgcgta ctttttccag actgcccgtg agcgtgggtt
    31801 tgataactac cgcgcgcacg ccctcttcag ccacaaaggc aaaatccggc tcgcgcaggc
    31861 tttcatccca ggcaatggca atgccggttt cacgggcaaa cgctcgcgaa tcatcgcggg
    31921 ttttgcacgg ctcttcgaga aacgcgatgc ggtggcgata atccgggtta acatatttgg
    31981 caaactgctg acctttcagc ggtgtccagg cgcgatttgc gtcaagacgc aaatgcagat
    32041 ccggaattgc ctccagcaac agattaacca ccatgccgtc gcgcacggct tcgtacaatc
    32101 ccactttgac cttcgccact ttctcgcctg gcatatcagc aagtttgagg atcagatcgt
    32161 caggatcgcc attacacagt ggtgccgcac ggtaattggc cgcctgcggg agcgtatctg
    32221 ccagctccgc caatgcacag cttacgccaa aggcaacgga aggcatctgc ggtagctcgc
    32281 catcgcctgc cagccagtta tttacccaag caagcagcac aatttgcgcc tcttcccagg
    32341 tttcctgact gaagcccggc agtggggaga tctcccccca cccttcgcgc tcgccttcac
    32401 gcaggcaaac atacagcccg tcgcgggttt ttaaccgcct gtcgcgcaga accatccccg
    32461 cgtccatggg gatctgccag cggtatacct gcgcgctacg cattacggat tccgtttaaa
    32521 tttgctgaaa tcaggctgac gtttctggtt gaaagcgttg cgaccttcct gaccttcttc
    32581 cgtcatgtag aacagcatgg tggcgttgcc cgccagctcc tgcagacccg cctgaccgtc
    32641 acagtcggcg ttcagcgcag ctttcaggca gcgcagcgcc atcgggctgt tttgcagcat
    32701 ttcgcggcac caacggacgg tttctttttc cagatccgcc agcggtacca cggtgttgac
    32761 aaggcccata tccagcgcct gttttgcatc gtactgacgg cacaggaacc agatttcacg
    32821 cgcttttttc tgtccgacga tgcgagccat gtaggaagcg ccccagccgc cgtcgaagga
    32881 accgactttc gggccagtct gaccgaagat ggcattatct gccgcgatag tcaggtcgca
    32941 catcatgtgc agaacgtgac caccgccgat ggagtagcca gccaccatcg cgacaaccgg
    33001 tttcggacag gtacggatct gacgctggaa gtccagcacg ttcaggtgat gtacgccgga
    33061 atcatcttta tagccgccgt aatcaccacg cactttctgg tcaccaccgg agcagaacgc
    33121 tttatcgcct gcgccagtca gaatgatcac gccgacgttg tcgtcataac gcgcatctgc
    33181 cagtgcctgg atcatctctt ttaccgtcag aggacggaag gcattgcgca cctgcggacg
    33241 attaatggtg atttttgcga taccgtcggt ggatttttca taacgaatgt cctcaaaacc
    33301 ttcggagcag tcgtgccatt caaccggtgc gtaaagcatt gcttcatcag gataaatcat
    33361 attgtgtcct ttagtcagaa acgcaagatc tgcgccagac ttgcgataac gccagcggga
    33421 ttttcccgat gcgcgttatg tccggcgcga ggaatgacat ggcagtcggc agccagttcc
    33481 gccgccaagg cgcggaattt gctgtcacgt tcaccacata aataataaaa cgcaaatgtg
    33541 cgggcgctaa ggttagcacg taaatcaggc tggacggcga gagaagtcgc ctccagcatg
    33601 gcggcaaggg ttgcgccatt attgttactg cgcagcgcca ccagctcccg gcgttgatcg
    33661 tcattgagcg aggcaaaaac aggctgttga taccagtcgg caaataccgc cgttaacggt
    33721 tctgtgcgaa aacgctgcgc ccattggcga tcggaacgct gacgttccgc gcgttgttca
    33781 gcattttgca acccaggatg cccgccttcg acaacaaccc cacaaagccc cggcatcccc
    33841 tggcaagccg ccatcatcgc cacccgtccc ccaagcgagt accccaccaa ccagaagtta
    33901 aggatgttgt aactaaccag ggttttacgc agtaagtcgg tgacatcatc aaatccatcg
    33961 acgctaatcg ccgccgaacc accgtgacct gggagataaa catacaaccg tgagtagtcg
    34021 gcaaacgcct cgcccacttc ttgccattcg tggcaatcgc cggaaaaacc gtgcaaaaac
    34081 accagccagg gtaaacctgg tttgccgtgt tttgcctgcg cgtgcaggat cataaatggc
    34141 ttacctgcgc cagaagttgc tggagcgttt gcgcaccatc ggtgtcgtta accaccattt
    34201 caattaccgt ggtggttggc gtgcgccagg catcggcaag tgccgtttca agttcctgcc
    34261 agttttgcgg acgatgatat ttcagctcga acatcgcggc ggcgtgctca aaatggacgt
    34321 tttgcggcat cagatagaaa cgctcgcgct cgcttttcgg cgttggcagc agcgagaaaa
    34381 tttgcccgcc gttgttgttc accacaatta atatcagcgg cgcggaaacc tgacgcaata
    34441 acgccagcgc gttgagatcg taaagtgcgg agagatcgcc cacaatcgcc agcgtcggtt
    34501 tgccgcttgc ccgctgaacg ccggcggcag tcgagagcag cccgtcgata ccgctggccc
    34561 cacggttgct gtacaccggg taacctgccg gaagttgcga aagcgcatca atcagacgta
    34621 ccaccaggct gttaccgaca aacaattgcc cctgttcagg cagatagtcg ctgatgcgat
    34681 gcgccagttg cgcttcgcca aacgcatcgc gacgggcaat aaccgcctgc attgcctgtt
    34741 ccgccaggcg cgggatttca acgcaccagg gctggcgttt ttctgccgga tgcagctcca
    34801 gccagtcggc aatattggca attaagcggc gtccacgatg gtgcgccgga tcaagccgcc
    34861 cttcgatgtc atccacaatc cagtactctt cgggttcaca gcttgcctgc cattgcagga
    34921 gccgtttgcc cgtcaggctg cttcccagtt gcaccacaat ttgcgcctgt tgcagttcgc
    34981 tggtcgcttt ggcattgcct aaccagagat cggcacacgg cagcggctgt ccggtttgcg
    35041 acagcacgtc gccaatcagc ggccagccaa gagtttgcgc ccacagtgca acttttttgc
    35101 cctcttccgc actcatgcgc ccggcaacca ccacgccgcg tttttgtcgc cagaagaacc
    35161 agtcgcgctg tttttcactt tccagacgag gcgcttcacg cagccacggt ttgtcgtcct
    35221 gccaccagtc gcccagccgc tgttgccagc taagcccggt gtcgtccatt tcgccataca
    35281 gcggttcggc aaacgggcag ttgatatgga ctccaccaga atgaagcgta ccgagagcat
    35341 ggtcgatggt agaaaccagc caacgtgcgg ggatatcctg ggtcgggcgc ggcaatgaaa
    35401 tactgtgcgt ggggtgagag gcgaacattc ccggctgacg aatcgcctga ttcgcgccgc
    35461 agtcaatcag ctccggcggg cgatcggcgg ttaacaggat cagtttttct ccggttaacc
    35521 cagcttcaat cagtgccgga tagagatttg ctaccgccgt accggaggtc acaatcaccg
    35581 ccaccggctg cttgctgact ttcgccagcc ccagcgccag atgccccaac ccacgctcat
    35641 cgaaatgggt gtggtgaatg aatgcggaat tctccgccgc cgctaacgtt aacggtgtag
    35701 aacgcgagcc aggggcgata cagatgtgtc tgacgccgtg acgcgttaat gcttccagaa
    35761 tgaccgccgc ccagcgtcgg ttaaatgcgc ttactgacat gagtttgtcc ggtatcaata
    35821 ttgcggctaa gtataaggag ctacaaaaat agattattga tatgaatcgg taatgatgcg
    35881 actcattact attccatctg taataaagta cgcagccctg ccgctttgtt gtcgatttcc
    35941 tgccactctt gctcggggtc ggaaccacgg acaatgcccg cgccagcata taatcgcacg
    36001 acattgccgc taatttttgc tgagcgcagg gaaacgcaga attcgctttg ctgtaatgag
    36061 agatagcccg cagaaccggc gtaccattct cgggtgaacg gttcgtgacg ggcgataaac
    36121 tgtcgcgcca gatcgcgcgg taagccagca actgctgccg ttggctgcaa ctgatgtaaa
    36181 cagatcacat tatccgcttt gttgagtgaa gtccagatac agcggcgaag atgctgcact
    36241 ttacgcagac gcagtacctg cggcggtaaa acatccagcg tctgggtatc ggcctgtaac
    36301 cgttggcaga tatcttccac caccagcata ttctcgcgct ggtttttatc atccgccatc
    36361 agccactcac ctaactgctg cgcctgctta tcatcaggat gatttgctac cgttcccgcc
    36421 agcgcttcag tacgcagcgc tttgtcacgc cgccgccata accgttccgg tgaagagcca
    36481 agaaaagcat tttcgccatc aaaggccatg taaaaatggt agcaattcag attcagtcga
    36541 cgactggcag ccatcatcgc cgccgcgttg accggacttg cgaaatgcag gtcagttgcc
    36601 cgagcgagca ccactttgtc gagctcacct tcggcgatgg ttttcgttgc cagttcgatt
    36661 aattgcgtcc agcccgtttt gtccggccag tgttgttctc gcgtggtggt taaatgtaac
    36721 ccaggcaagg gcttgatact caccagtgtg gcgataaatt ctcttgcctt aatcgcatcg
    36781 tgctgaaggg agctttcgct gaatagcgtc agccgcagcg tggctttacc gccacagcgt
    36841 cgccattcca ggcgtggtaa aagtaaattg ccctgcgacg ggtcaaacgc attcagcccc
    36901 caaatgcgta agtcggcgtg ttccgggtgc tggcgaagaa aacgttgtgc ctggtccaac
    36961 gacgtaaaac gggtaatcgc gcccaggacg gcagcttctt catcaccatt acgttgttgc
    37021 cagtagaatt gcgggtatgt ttgctgactg gccagccagc tcaaggcatc aaaagcgtct
    37081 ttgagaggga aaggaatatc gataacccga atgccgggtg tcgccggaat ctcttgcgac
    37141 aaatggcgca gtagattttc cagcgccgta gtaagtgatt gcacgcggac ctctccctgt
    37201 taaaagcctc acattatacg gggtactaca aaaaaaatgc agtaccccgg tgtagggagg
    37261 ttttaacggc gtgccagcaa cagtcctagt accagcccaa cggccgcgcc cacaccaatt
    37321 ccttgccagg gtttttcgtg gacgtagtca tcagcacgat aaactgcctg cttcgcccga
    37381 tagtaataac tgtctgaagc ctggctaacc cgttttttca catcatccag cgctttttct
    37441 gcacgcgctt tcagctcaac atatttctga tcggcgggat cgccagagga gcggagcacc
    37501 tcttccagtg tttcactaag cagcgtcagg tcgtcatcga tacgtgtatc accaaactga
    37561 ttagacattc tcgttctcca tttgcgtaaa acctgtcact aactatagac aagggttgtt
    37621 gattacgcct gaattacctc gcgtgccatc ccaatgtgtg ggataccatc ttcttcatag
    37681 acctccgtca cagggataaa accgaaactc tggtagaagt tttgcagatg cgcctgcgcc
    37741 ccgaggtaaa caggcttatc aggccagtga tgcgtgcagg tgtcgagggt tttactcatc
    37801 agttgctgcc ccactttttc gccacgcaac gcttcactga caatcacccg accaataacg
    37861 accggctcaa gatcatcatc acttttcaga atcctcgcat atgccaccag ttcatcattt
    37921 ttccaaccga gaatatggcg attatccccc gtcaaatcat caccatcgat atcctgataa
    37981 gggcaattct gctcgacaac aaacaccgcg caacgcagtt gtaataaggc ataaagctgg
    38041 gaaaccgata attcagagtg atgcagatct tgccattcaa tcataatgct ctccttttag
    38101 atgtatgcct tatactagcc ggaatatttt ttgaaacgtg atgaactcat ggaattaatt
    38161 tttttaggta cttcagccgg tgtgccaacc cgcacgcgca atgtcacggc aatattgctt
    38221 aacctgcaac atccgaccca gagcggactt tggttgtttg actgcggtga aggcacccag
    38281 catcagctac tgcataccgc ctttaaccct gggaaactgg acaagatttt tatcagtcac
    38341 cttcatggcg atcatctctt tggtttaccc ggcttgctgt gcagtcgttc tatgtcaggc
    38401 attatccaac ccttaacgat ttatggtccg caaggtatcc gtgaatttgt ggaaaccgcg
    38461 ctgcggatta gcggctcatg gaccgattat ccgctggaaa ttgtcgaaat tggcgctggc
    38521 gaaattctcg atgatggcct gcgcaaagtg accgcttatc cgatggaaca cccactggaa
    38581 tgttatggct atcgtattga agaacatgat aaaccgggtg cattaaatgc ccaggcatta
    38641 aaagctgctg gcgtgccgcc tggcccactg tttcaggaat taaaagcggg caaaacaatc
    38701 acgctggaag atggaaggca gattaacggc gcagattact tagctgctcc agtgccaggt
    38761 aaagcgctcg ctattttcgg cgataccggc ccctgcgatg ccgcacttga cctggctaaa
    38821 ggtgtcgatg tcatggtgca cgaagcgacg ctggatataa ccatggaagc caaagccaat
    38881 agtcgcggcc atagctctac acgccaggct gcgacactag cccgtgaggc tggagtcggc
    38941 aagctaatca ttacccacgt cagctcgcgc tatgatgaca aaggttgtca acacctgtta
    39001 cgtgaatgca ggtcaatttt cccggcgact gaactggcga atgatttcac cgtgtttaac
    39061 gtttaa
//
LOCUS       DAQSWQ010000031        38647 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.031, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000031 DAQSWQ010000000
VERSION     DAQSWQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.031"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            438..1460
                     /locus_tag="R8G18_001640"
     CDS             438..1460
                     /locus_tag="R8G18_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEE9795362.1"
                     /translation="MASLNRPSLHWSELAPEEQILFWEDYESGRATSFLVEPEKRSTK
                     RRRGGHSTKPKCENPSWYRPERYKALPGQIGHAYNRLVKKDPDTGELSLRMHISRHPL
                     FVARRREAGRTYKFRPEKERLLDFLWPVLVSFSDVGTHTVGMCISRLAKELSLKDEQG
                     RVIPETEVTVSRVSRYIQEQVRFGVLGVSEETMWDRETRQRLPKYVWITDLGWQMLGV
                     NLEKLHEQQKKRLHESEIRQQLIHLGILGEEEEISVHAARKRWYEQRSLDALKFRREK
                     AAAAKRAKKLQPLSDDERIQVMAEHILKCMPPDEAYWCTPERLHKLAVRELCQLKLFM
                     SASPPH"
     gene            complement(2530..2832)
                     /locus_tag="R8G18_001641"
     CDS             complement(2530..2832)
                     /locus_tag="R8G18_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795363.1"
                     /translation="MAKLPKKINQEIKALDLRQFVSSAAQKPASASTVTRINLTMTDT
                     DLDIAQEFQNEYGASRAEVIRTALAALRLISAEERRILFDDIRKNSPKAGRPTVNK"
     gene            complement(2922..3581)
                     /locus_tag="R8G18_001642"
     CDS             complement(2922..3581)
                     /locus_tag="R8G18_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="HEE9795364.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCVIKKGKTACFLETDSQGS
                     IKDFIEERQANASLSEIPYYECYTDIPAMVQKLAARFDYVFVDTPGMKSPAFVKALSC
                     ADIVLTFVEPGSGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCATNPSDSEASEL
                     RNQLNDDPDWLPVPRQRIYMRTAHKKAYNCGMGVHEYNDKRGNKARGEIELLLKETNI
                     I"
     gene            4134..4457
                     /locus_tag="R8G18_001643"
     CDS             4134..4457
                     /locus_tag="R8G18_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795365.1"
                     /translation="MENYAKFVATEILKQLGGNRFIAMTGATKFAYFDENGECGLFFR
                     LPSNFAMKGINLVKIKLTFSDTYLVTFFRIRGTTKKEISKFDNIYCDQLESLFSEETG
                     LATRF"
     gene            4811..5155
                     /locus_tag="R8G18_001644"
     CDS             4811..5155
                     /locus_tag="R8G18_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEE9795366.1"
                     /translation="MPYSAKRYQTENGEVPYTDWMKKLRRKDQTAALKVDSRIARAMS
                     GNFGDHKFERDGVWELRVDYGPGYRVYYSIEDGEIILLLIGGNKKTQTVDLDKAVSYL
                     QDFKKRSKNDSH"
     gene            5142..5492
                     /locus_tag="R8G18_001645"
     CDS             5142..5492
                     /locus_tag="R8G18_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEE9795367.1"
                     /translation="MTATKNVKSQKNDYPASVDHNAAMIEELRADPDYANAYLANALE
                     EINEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLT
                     SVAGLQFTLSKSSH"
     gene            6326..6841
                     /locus_tag="R8G18_001646"
     CDS             6326..6841
                     /locus_tag="R8G18_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEE9795368.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELM
                     KAVNAAFDVLMANIDKINQFQSADEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVW
                     ISGETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRR
                     ATGWQRVETRA"
     gene            6945..7487
                     /locus_tag="R8G18_001647"
     CDS             6945..7487
                     /locus_tag="R8G18_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795369.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCPSRIKLTSPQFKN
                     YSVHIRMEKDRFSVVGSVDSRSWSSPYHVCTLSRKRNPVDIAANIERKILLNASQEVL
                     QAIEYEKRQAAKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGERYDL
                     QIRGLSIDQLVKITGYLKQL"
     gene            7484..7738
                     /locus_tag="R8G18_001648"
     CDS             7484..7738
                     /locus_tag="R8G18_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795370.1"
                     /translation="MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTL
                     SELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYKESDA"
     gene            7735..7977
                     /locus_tag="R8G18_001649"
     CDS             7735..7977
                     /locus_tag="R8G18_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEE9795371.1"
                     /translation="MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHN
                     QLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP"
     gene            8548..8841
                     /locus_tag="R8G18_001650"
     CDS             8548..8841
                     /locus_tag="R8G18_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795372.1"
                     /translation="MKFTSTTNHVFTFERVTLCTIVLIHKDTGQQYVVIFTDNNKIHD
                     YKTGIVSQFGELKQSDIDLILFYRDEYEKYFDSLNNGEEYLSFKEYIGCIRGK"
     gene            9148..9435
                     /locus_tag="R8G18_001651"
     CDS             9148..9435
                     /locus_tag="R8G18_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795373.1"
                     /translation="MINEAKKIAVEMMFWTACPVDDDNCFDAIINASKIDREFEDKHA
                     IYLHPIYRDLLSTDQIKSALKKRAIKIISFKCDINEDSARKILMQTILRAV"
     gene            9863..10096
                     /locus_tag="R8G18_001652"
     CDS             9863..10096
                     /locus_tag="R8G18_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795374.1"
                     /translation="MSSEVIKQIQKIQDRGIIIYSKFRAAEFDQDDVYRESYFLVVEF
                     NELIAENIIHDEKLVDQTACILHELRRIAIEGK"
     gene            10172..10474
                     /locus_tag="R8G18_001653"
     CDS             10172..10474
                     /locus_tag="R8G18_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcgAII protein"
                     /protein_id="HEE9795375.1"
                     /translation="MDTFDSEKFLSELDVLSKESMPGCGLVFELYQRRLSAAIDKVVA
                     GLPEEQHAQAFELARQEFDYLSAEEIAEEIRRDSERGYCCHGFDRDCCPLGCGDLP"
     gene            complement(10791..11468)
                     /locus_tag="R8G18_001654"
     CDS             complement(10791..11468)
                     /locus_tag="R8G18_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795376.1"
                     /translation="MINDKSFNIENIISDIFKETRLKISKDDPVLSIILMHEKILEHA
                     LTQLKNSNQIATERLSHDISSIRDAINALPDAIDEKTSELQHAAVALHDEFQESKGEI
                     KGSLEEARINATEKLAESAKELQLNITKVAEKTTETIESANKIISAIDTNLAEINKKA
                     LANYVNDIRSLEKKGESISKNIDTAINNAFKSSVKSFKFYCGAALFISTVLQFTMWGF
                     FLYKLLT"
     gene            complement(11518..12273)
                     /locus_tag="R8G18_001655"
     CDS             complement(11518..12273)
                     /locus_tag="R8G18_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HEE9795377.1"
                     /translation="MKNSINFILQGKGGVGKSFTTAILAQYFIDEKNMDNVVVGDTDP
                     VNTTTAKVKRLNADLIQITENSKVVQSKFDPMFESMLTNTQNTFVIDNGASTFLPLIQ
                     YFNDNCVMDMFNEVEQEVYIHTIIVGGQAQADTIEGFEDLVKLVKGTKVKIIVWINEF
                     QGVPALENIPLIKTKFIEKNKDVIAGVVVIQDRKSDAFASDIKNLTEKSLTLKEALES
                     EFFGLMAKSRLKRVFNDIYVQLDSIYNMQHTEA"
     gene            complement(12291..12692)
                     /locus_tag="R8G18_001656"
     CDS             complement(12291..12692)
                     /locus_tag="R8G18_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HEE9795378.1"
                     /translation="MKKIDSLLSPICEAIKETNSKSKQAVFNMYLSNILEAKSYYTWE
                     QITDYINNKTKTELAQKTYINMTERAKKRQKCTGQKDLKKSTNMIEKKIDEDNKQRGG
                     VKGFFSTISENKNEIDHDPSATNEKFINKYL"
     gene            13036..13440
                     /locus_tag="R8G18_001657"
     CDS             13036..13440
                     /locus_tag="R8G18_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEE9795379.1"
                     /translation="MEVLRTMKKQDKGKKAVASTSKVERKHVISLRLTDEEYQPFHEY
                     FTSLEVSRSEFFRMVILNRLDDIPTLKQKRPVEYRELLRLFNKSSNNINQIAKKINTE
                     YRNSFISESTYLRYLNVLVNIRDTFVAGINKC"
     gene            13434..16718
                     /locus_tag="R8G18_001658"
     CDS             13434..16718
                     /locus_tag="R8G18_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795380.1"
                     /translation="MLVRISGGNKGVCEYLEKGHKQGREYTRDEIDHRLVLDGDLALT
                     ESVIDAIPDNGQERYMHITLSFFEDDVSESKLHDVVQEYKSLMMNAYHEDEYNFYAEA
                     HLPKIKQMPDHKTGEMVERRPHIHIVIPEVNLVTGSKLNPRGMTEHYIQYLDAIQEHI
                     NNKYNLVSPKDGVRVSDHNHANVLARAKGDFYRERHSEFKTTVFDALDTENIRTEKQF
                     SDYLSRFGEVKEYNKGKSERYFGVKLEGDSKFTRLKSPLFSQQYISDRTIPLVKPTKT
                     QIENRLKEWKSSVSHEIKHIHPAGQKIRKEYAALAGEDKQLYLKKVINRYDEKHNLQR
                     QDRRQNSDQPGVKYNTGKSVSGKTIGLPRLPTRELVYGLHGRSDGKSINKSQLLLQQN
                     EYGHLSETGRQEVDPDRYVRRLQDRAGRRVVQSPLESKIKAAIQRNDDLPFLKQIRAE
                     INAERYLSNLQTRFNIDPSKHTVTYAPDGSPRFRVGNRNMNASDFLTKHLNLEWDEAK
                     EVLLDIWQDQKDNKPFDAVITRVSLTRQQAKERFICLNEHKGWVNNEIKYKHNDNIAT
                     YRAELKGLLAIRNKQEREVARGYIIFKKLERKREINSIRKACFREINSYYHRWNPDRR
                     YDMALGDSLKKIIRNPVDGNSISSGNEKEVFSVVKRVQQSQQVQDYVKRELTDLVPHK
                     VSPKETRYLDPKTTQTVFIDKGNHIRVNGEITQDKAEAALLYAKEKFGGMLKLNGSEE
                     FKVACAMAAAEKGMNVILRPEKYHEMMQVRLQELKAEHGHENSVEAERQQQVAGQKPE
                     QEKRAEQVNSVEKDTQAKERAVEVAESVRNDDKSRDAVPEHPVKSETVSQKSTSETPV
                     VQEAEKQESVLQNVSAKSEEREQQPQESQQQKPVTEKDVATGQEKQTGNEQSTESALT
                     TENPQQQNNASLQHLQEEAKVIASQHKEAQRLLHEAVENGAPKEEVDLLSKGVNSISA
                     RAQTINEQIKAFEEQQGKKKAKSVEEKNENTTEKKKRTVNSEKAMEREIERVSKQLAK
                     EMNIDKQTVKDTLKFIRFEDVNDLKAAMKNPETVEAVKREIVSYDNALKNERERERSR
                     DNTRTR"
     gene            16946..17956
                     /locus_tag="R8G18_001659"
     CDS             16946..17956
                     /locus_tag="R8G18_001659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795381.1"
                     /translation="MDIYIDYNVYLNILNKEDDYEWVVAKLNNLRKSTSFIVYSPAHI
                     EEIAENRSRDNVDILKVMNKTMHIISRYTRNVEYLPGIMTNDQIKDLLFQLSDNPAAI
                     STRLNLEKMLFKNLISGISDDDCKTRRVIESPRDCMKRVYGLIDQTDVAHKNDIYHIG
                     RRNDKSLCDNFTQLGIEYDSIQTFESYQKEKKLGPKRLSNIAPQDIFNDEHIIEEFNI
                     KLQSSGFMKKPTQEQLSNSHQLRSDIITLTLNFLEQIGYKQERNNSSYVIRSRMHDVS
                     HAIYGANADYFVTFDKRLRDKLVATYEFLNIPCKVMDKNEFICHNFSHPVVINIGANA
                     VP"
     gene            complement(19475..21640)
                     /locus_tag="R8G18_001660"
     CDS             complement(19475..21640)
                     /locus_tag="R8G18_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEE9795382.1"
                     /translation="MRLFIAEKPAVANDIIKALGGNFTRHDGWFESDNAMVTNCFGHI
                     IESQPPENYNPEYKIWKVETLPLRLYPVKYQPVERASKQLKTIIELINRADVTEIIHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKAL
                     ARSVADEIYGFSMTRAYTIPAQNKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSVF
                     YTMTGYFQRGADVIRANWKPGEFAPLTDRKLLDKAWANGTAASLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNRKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     CEAPQIIDALKAVFPQQLDINSARKSKAFNSAKITAHTAIIPTVSVPDINSLSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGE
                     SEDNDDSAFELLCKIRTGETLTTKEVVVNEKKTTPPPLFTEASLLAALVRVADFVTDP
                     TIKKLLKDKDKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDA
                     LPDIAVNPDMTALWAEKQAAIENGDLTVEEFINELYSELSSMITDVDLGEMKIEAAAP
                     AGQSQRLNAPCPHCGKQIAIRPKGYFCTGCEFKIWKNFSGKALSDKQVETLLTKGITG
                     ELKGFVSSRTNKEFSAKVKLIDKATGKLGFEFSPKNSDC"
     gene            complement(21645..22220)
                     /locus_tag="R8G18_001661"
     CDS             complement(21645..22220)
                     /locus_tag="R8G18_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEE9795383.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENLVKELPDIDESVHPF
                     HVCYGGYLLNPGVSYSDISRKIKDQTGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGH
                     QKIVPFAALIKADECIISKIASYSGFAVTAFLRIKDQDIATNILNREGIFAFNGCERR
                     FRQPVSEDNWQQAVSEERAIRCANRLIQCKG"
     gene            complement(22245..22832)
                     /locus_tag="R8G18_001662"
     CDS             complement(22245..22832)
                     /locus_tag="R8G18_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795384.1"
                     /translation="MYLHIVPKLFHRMANKCTLKSIVIPELDLIIDGESLSVGRPWPN
                     KCVWVGMLKGRKSVNGLILQTEKKLRWFTTRYTWDIEDMGLIYHQINTYIKDNEFDMV
                     SQEILLNGSFDKWSDRVHSAYENKPPARIQPKMESLLNKPGENSHDMWKEFEWGDFLL
                     SREESLLLHTIQSERLNTDFSLFKRQPSIESALVI"
     gene            complement(22914..23195)
                     /locus_tag="R8G18_001663"
     CDS             complement(22914..23195)
                     /locus_tag="R8G18_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilI"
                     /protein_id="HEE9795385.1"
                     /translation="MKIRVVSNSGQERIINLNNLTIELLSDNDLAKVTKSLRTPKNRM
                     VFIIQDGNRILRWDRSYASHTKNHWRKVAPDSFEVLGTIENLHYFGKQN"
     gene            complement(23379..23723)
                     /locus_tag="R8G18_001664"
     CDS             complement(23379..23723)
                     /locus_tag="R8G18_001664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795386.1"
                     /translation="MTFIRINRQREFIERKHSSRLTLRKDKNRKGIVSCYLSKKVRGD
                     GIFVIIELDPEHKTIRIKTTNISTAKGLKMSRGTFSLSKVWEHMFYKNLHEDQIIIEL
                     KEKDDGWWYGTF"
     gene            complement(23782..23991)
                     /locus_tag="R8G18_001665"
     CDS             complement(23782..23991)
                     /locus_tag="R8G18_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795387.1"
                     /translation="MAEKVQGAPQPDCIPINQRFFSIRAYTPFYNPGATYVARQKFLY
                     TCSGSPANQTILNMIMQRYGRSFSA"
     gene            complement(24336..26357)
                     /locus_tag="R8G18_001666"
     CDS             complement(24336..26357)
                     /locus_tag="R8G18_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEE9795388.1"
                     /translation="MKKKKLVLPVLIVWLMALTASGWLGSFILVKIASLPTDNLQWNT
                     YFSTLAYYGWQPSLKKYLFAGLAGTLLPFLIALVLTVVMFVAVQEKKTVHGNARLAND
                     RDLADSGFFPDEEELKNLKHPSLLIGKMPENKGRFKSRYLLYSGQQFLMLYAPTRSGK
                     GVAIVIPNGIWYTESMVFLDIKLENFLETAGYRKNVLKQEVYLFSPDGYAREIGGETV
                     LTTHRYNPLYYIRRDPVYRFGDLEKIAVILFPMTGGENDTWVESSKNVFISLVLWLLD
                     TELEGKFKVNMNSVMKLSIPTDGSDLAKWMLNEIRSRGNEVLINRWKEYHALPEEEKL
                     GKAPSTYPLSDDTIRLFRQFAAKEPKQQESIMLDFNRTMSMFANPVVAAATEDNDFDM
                     RDVRRKKMTIYYGISPGALKQYQRLTNLFFSQLLNENVRTLPEHDATLKYQCTLMLDE
                     FTSMGRLDVVQVSLAFTAGYNLRFVFILQNREQLFDEKHGYGKMGGNTILKNCAVEIF
                     YPPKKVDESVKDVSETIGYYDFVRTQISRTTGKNPSTTRNKIIEKRAVMLPQEITDLR
                     DQKFKVKGKKTSFSLPQIVMSEFCRPFIAHKIVSFDQKNEPFFVNARKYSRKNIIDTP
                     VLDIPQDEVVRQLMLQREREEAWKAAQPAFDKDDFDDEKEYFSDGDSGD"
     gene            complement(26678..27691)
                     /gene="virB11"
                     /locus_tag="R8G18_001667"
     CDS             complement(26678..27691)
                     /gene="virB11"
                     /locus_tag="R8G18_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HEE9795389.1"
                     /translation="METINMFLESTGVRPFLQAEGLTEIIVNRPFEIITEGVNGWEYH
                     EAPGASYSDLLDLSIAINKYNNSAEPLDALHPIKSLVMPGGERMQIIMPPACEERCIS
                     FTIRKPSLKRFTLEDYVSSNRFSNVHIVNQANLSTLSASQQKLLDLLDNTRCNPRTWI
                     SFLEAAVAARMNILVVGGTGSGKTTIAKTIADLFPHDRRIITIEDVHEMALPNHRNHV
                     HLFYKQGGVQPRYLIEAAMRMKPDHIFLAELRGDEAWSYLEALNTGHEGSVTTIHANS
                     ALDAISRLASIVKQSTVGLTLDHSFILKTIKTSIDIVVFFKHTHMTEIYYNPIEKNQL
                     LAV"
     gene            complement(27691..28890)
                     /locus_tag="R8G18_001668"
     CDS             complement(27691..28890)
                     /locus_tag="R8G18_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEE9795390.1"
                     /translation="MKNNNDIVIEKLDEIDTQEADTDAQNRNFIHSGKKQGNKKAKAV
                     LILAGFSFVTVVILTAVLSKVFSASGEEETTETATTKAQAEVYAQNTQAKNLDDLKES
                     VARSIEEVKNNVNSEEEQVREETRPENKGVTLPTENETDAGYAQSNGDAPPTPAQRRL
                     MGSVMVDDNRISQSQPGSSGQKTAGATSATSDNGDFLQGADFADGSVSRIKNRRFLLS
                     AGTTFPCVLKTKIVTTYPGVTLCQLTKDVISDDGKTVLIRAGAQLQGEQTQAITQGMA
                     RVFVNWTIVKDKNNRVRIDSLGADSLGASGLPAWVDTHFWERFGGALMLSLIEDSLAA
                     GTSRLSRENNSDFTMNNTTGTSSELAKTTLDNSINIPPTGYVNQGEMLTVIVPRNIDF
                     SSVYKVR"
     gene            complement(28880..29677)
                     /locus_tag="R8G18_001669"
     CDS             complement(28880..29677)
                     /locus_tag="R8G18_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HEE9795391.1"
                     /translation="MMMRKLLLSLVISGITMTCHAAKTPASAGFDRRVQQIVYNPNDV
                     TIVKTKAGEVTLIELEEGEIIKSEDTGLAIGDPEAWDPAVRGNTIFLRPKAAEPDTNI
                     AIVTNKRIYSIQLVSTDQNPTYLLRYIYPKPPEPVKPNVMTGRSRVVAPCTEGNIVNG
                     RYEVRGSRKIKPEAIWDNGRLTCFRWTGAADLPVVFRILPDGKEQLVNYHMDKNIMVV
                     HEISPAFVLRLGGEVMEVRTNRHIPRTWNASGTLTGETRVEINNDEK"
     gene            complement(29674..30420)
                     /locus_tag="R8G18_001670"
     CDS             complement(29674..30420)
                     /locus_tag="R8G18_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEE9795392.1"
                     /translation="MGLKEYFKRSKAEARKTPAQKETDKKIEKNVLDAAKEFARDRDN
                     ELKRSLRITRFALFFSSASAAAMAVALAVMAPLKTTEPFLLRVDKTTGYTDVITPFNQ
                     DSGTYDEAVSRYFLTRYLENREGYEWYTIQNMLNTVELMSSSQVFAEYNNMIHGPLSP
                     LYKLKKSYKILVRVQSITFLDENTVQGRFVKAITDPDGKPAPGYTPTQWIATIKFVWL
                     KDKIATEAERQINPFGLDVLSYRVDPEVVK"
     gene            complement(30621..31667)
                     /locus_tag="R8G18_001671"
     CDS             complement(30621..31667)
                     /locus_tag="R8G18_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEE9795393.1"
                     /translation="MASNDSAFAKANFEIVQPMFNELSSIIQNSVTTNFQTIMQLVTP
                     LFGAAVILYGVFFAWEIMYTRKDDVMMESVKRMGVLTLVFAMSTAGGMYLSYIVPFVQ
                     GSGQEVASRIIGQDGASTGAVIDGLLNRLIDLGIQQFQVFKDTEGWFDSMGAWIIFIL
                     KAILLALAGGGFVIYCAAYLLMAEMMVGILLSIGGVFILFSAWPTTRNMFTAWVGSCF
                     NYIFLNIAYACLFKVLIQYINKYINTNSDDITKNIWSVLCVAVVFMIGKFLLAQISTL
                     ISTLTGGVGINGLTSATGDVIKALRGAGNSTRNQLGLGFNPTAQNARDRNRELAVAAK
                     DYLSSRFGGNIKAG"
     gene            complement(31733..32086)
                     /locus_tag="R8G18_001672"
     CDS             complement(31733..32086)
                     /locus_tag="R8G18_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795394.1"
                     /translation="MFKKLLGGFILAFVFLLSGCGGDNGELAGTYIEKNPASGGAPVK
                     WIIKYDNKDNYYSQEIYRKFSPNGEYSNSNTNTFLVRDGDWIGGEKEKRFIRVIDRNT
                     LQFRDDENDLYIRTN"
     gene            complement(32180..32848)
                     /locus_tag="R8G18_001673"
     CDS             complement(32180..32848)
                     /locus_tag="R8G18_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795395.1"
                     /translation="MKKTLTALFLGATLTGYSSVASSVGGVIVKDPGSIAKTVEVIKK
                     AADQIAELKKQVDLATSQLEAYKQEVLDTKRRLEGITDYSAIFGSAESYMKDFWEDMK
                     KELTDADIRSMATKYGFDTKEYDRIKRQYESKFEEITKYEAMSKDLEKSAEKIKATQK
                     AFSKADTPQKREELQNNILLETAAMQLKIAQNEAEINRMARERKLREEAALMKYTEDN
                     FSFN"
     gene            complement(33066..35507)
                     /locus_tag="R8G18_001674"
     CDS             complement(33066..35507)
                     /locus_tag="R8G18_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB4"
                     /protein_id="HEE9795396.1"
                     /translation="MSIKKITNNFIPPYHSHIHENIIWLEDKKLLLTFLIEGIPYESL
                     TDDVILNNFNGQKETLLGLCKSERVFLWKHLVKRESSMDARYNFPSNPFLQRLGDFYC
                     DTFNGERSFRTDYFLTIGIPYDDIDAGEKKAKDIIRQIETGFKDYNIYTLGISDGGIS
                     DICSNYLNYLLNHDFEKIPLSATKISESIKNSKFYFSFDTLEIKNRTGHDNKFCSNYI
                     VKDVPRGSQIGQWNFLLSLPFEFILTQSFIPDSLSKSSKKIEQQLNKLTSVKDAGKTQ
                     HTELHMAQEAIINQETVFGSWHSVLSVFGNTPDEASDNGSEVAAEFVTSGRGFRFVRA
                     ASEAPYAWFSHLPASKLRPLATQRTLTNVCCLMPLHNHSYGKKSGNPIGDGSAIMPLK
                     TVTDGLYWFNTHYSAPGRNVTGQMIAGHGMLLGATGAGKTTFEMMAAAFIQRFNPDMF
                     VIDFNRSTELAIRMFGGQYFSLSEGVYSGLNPFQIGDNDDPELMAFLKEWVKRCAVNN
                     DGSDCSDSEAEEIDRAVEMVMKLERRQRRFARLQSRIQSPELNVRLSKWWDNGALAWA
                     TDSEENKFDPDQYSKIGFDTTVILETVNQRDHPACEVILSVLFFYKKRMQKGGKLMLS
                     IVEEFWKPCNYPMTQALIKASLKAGRMKGEMMWLTSQSPEDAINCQIFAALVQQTPTK
                     ILLPNPDAKYSGYKEIGLTEKEFRQLKKLKLESRTMLIKQSGSSVFAKMDLVGFDDFL
                     PVISCSMKGIKLCEKIREEIGSDDPELWYPPFIEGIYQIDDVIREANSDIPEVWLPFM
                     MKRLKDKLPEKSLSL"
     gene            complement(35509..35832)
                     /locus_tag="R8G18_001675"
     CDS             complement(35509..35832)
                     /locus_tag="R8G18_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HEE9795397.1"
                     /translation="MDKEEIKASEELKAIFLTYNGLNRPAMIKGMPIIPFILCLLALM
                     VSFFVGILTIGFYGLIIPSIIIGVMIAIKQMCADDPNAMSARTLKFKGLCLKGFRSNN
                     VLKVE"
     gene            complement(35887..36204)
                     /locus_tag="R8G18_001676"
     CDS             complement(35887..36204)
                     /locus_tag="R8G18_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795398.1"
                     /translation="MEKIVNKSIALYRTFFLFLLCSFIATNPALAAGVGGLDKGTNFI
                     GELRIWAYGFVGAIAILHILWKVFQAYTEMDSWSGVLKSLMYAAIAGGCVVAAEACYQ
                     IFK"
     gene            complement(36274..36855)
                     /locus_tag="R8G18_001677"
     CDS             complement(36274..36855)
                     /locus_tag="R8G18_001677"
                     /inference="COORDINATES: protein motif:HMM:NF013618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEE9795399.1"
                     /translation="MTLISQCAPGVSPETMRAIIMTESGGNPYAIANVTDGGSKYFTT
                     EEEAVNHAKKLTTDKKNFSAGLGQINSRNFQTLNLTHESVFSPCANIRAAAAVLKTCW
                     DKYDTKGGGQQKILRDAISCYYSGNNKRGYVKESNGKSYIDIIEEKAGIHTHYDIPAI
                     KSHSETSSHLMVSSDNHDKWDVFGDFGIQDFKE"
     gene            complement(36934..37423)
                     /locus_tag="R8G18_001678"
                     /pseudo
     CDS             complement(36934..37423)
                     /locus_tag="R8G18_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37679..37963)
                     /locus_tag="R8G18_001679"
     CDS             complement(37679..37963)
                     /locus_tag="R8G18_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795400.1"
                     /translation="MSQAKANLKKLRHRCYIPPKQKISSLYYVEKALSSPWIYRERPA
                     YRAELLLTHDILLVIYGLAKSGLIDRSEESLNREIKGVRKTLAAGRFRWS"
     gene            complement(37983..38210)
                     /locus_tag="R8G18_001680"
     CDS             complement(37983..38210)
                     /locus_tag="R8G18_001680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795401.1"
                     /translation="MREKHIQIQGMIMRDSNYLDGLIAGGLVAISFAGLIMLIVSDLN
                     NEAETEKNTAFYEWADSHCEKIEKNLNVTIK"
BASE COUNT        10883 a         7931 c         8417 g        11416 t
ORIGIN      
        1 aaaaaaaaat actttttggt tgttttgccg tcagtgtcag atacgcggat ttggtgggat
       61 tagtggaatg agtgtcacga caaacacttt tcaaatcgga gggctacagg tatattaagc
      121 ggcattcggc gttgatcttt tttgatcctc gcttaaattt tgcaggttat gagaaatttg
      181 gtccccgtca gaggggataa aggtaggatg cgccataagg cattcaggag tatggcagga
      241 gttgaaagat tccatgaagc cataaaaagc aaacccccat taactttgca tccgtttctt
      301 ggcagaggcg atcaaagttt aatgggggca gggaaaatcc cttggaagac agtatacatg
      361 atgctttcct cattattcaa tatcgtccgg tgtaaaaatg cactgtgcga atcaaactct
      421 gccataagcg attgcttatg gcttccctga acagaccttc attacactgg agtgaactgg
      481 ctcccgaaga gcaaatcctt ttctgggagg attacgaatc gggcagggcc acgtcatttc
      541 tggttgaacc ggaaaaacgg agcacgaagc gccgccgtgg tgggcactcc actaaaccca
      601 agtgtgaaaa tccgtcctgg tatcgtccag agcgctataa ggcgctgcct ggtcagatag
      661 gccatgccta caatcgactg gtaaaaaaag atcctgatac gggagagtta tcgctgcgta
      721 tgcatatctc ccgtcatcca ttgtttgtgg ccagacgcag ggaggcagga cgaacctata
      781 agtttcgtcc tgaaaaagag cggttgcttg atttcctctg gcctgttctg gtcagcttca
      841 gcgatgtcgg tacacacact gttggaatgt gtatttcacg actggcaaaa gaactcagcc
      901 tgaaagatga gcagggacgg gtgataccag aaaccgaggt tacagtgtca cgtgtgtctc
      961 gttatatcca ggagcaggtg cgttttggtg tgctgggggt atcagaggaa accatgtggg
     1021 accgtgaaac acgtcagcgt ttgcctaaat acgtctggat aaccgatttg ggctggcaaa
     1081 tgctgggcgt taatctggaa aagctacacg aacagcagaa aaagcggttg catgaaagcg
     1141 aaatccgcca gcaactgatt cacctgggga ttcttggcga ggaagaagaa atatccgttc
     1201 acgcagcacg taaacgctgg tatgaacaac gctctctcga tgcgctgaag tttcgccgtg
     1261 aaaaagctgc tgccgcaaag cgagctaaaa aacttcagcc attgtcagat gatgaacgaa
     1321 tccaggtaat ggcggagcat atactgaagt gtatgccgcc ggatgaagcg tactggtgta
     1381 cgccggaacg gctgcataag ctggcagttc gtgaactctg ccagttgaag ctgtttatga
     1441 gcgcgtctcc cccgcattag cggttaattc ctgaacaaaa aataatctct gcccttcggg
     1501 gctttttgtg ctggctataa ttggccagtt ttctctattt ccgcctctca gccctgatta
     1561 actcgtcatt cagccctgaa aaggagggaa tgttgcctct ggcaatcctg ttttccacaa
     1621 aaatctgcaa atgggaaagg atacaatact gcaaatgaag aaattcaaaa ctgcaatata
     1681 ttcttacctc ctcagttaca tcccccgctt cgttcctcgc gagaaaattg tttgtcctct
     1741 gtaactccgt gaacaaaaag aaaaatgcct tcgcttgcag cggccagtgg ccgcgccgct
     1801 cagaatttta aagtacctcc cccgcttacg cggtgggcat acgaacctct gcccagtgga
     1861 aaataaagtc cctcctaccc tccaacaaaa aaactctgca aatactcagc cacaacgccc
     1921 aacccatcac tgaaaagcgc cgccgccccc gcccgaaggg cgggaacaac atcgctttca
     1981 ataatggatg ttgtaactaa gaaatctcat cgctgtcagc cttctggctg gaagtaccaa
     2041 atacacgctc gtaagcggcc ctgacggtcc gctaacgcgg agatacgccc cgactgcggg
     2101 taaacccttg tcgggaccac tccgaccgcg cacataagct ctgtcatggc taaaagcggg
     2161 tatggcttag caggatgggg atgggtaagg tgaaatctgt caatcagtac cggcttacgc
     2221 cgggcttcgg cggttttagt cttgtgccat atgtagctac atcatgccgc cttccatgcc
     2281 gctggcgcgg catcaaaaag gggtccgctt ggaaaacgga aatttttcca cgctaagaga
     2341 agttccagat atattgatat atcacaggct ataaccttgt ctgctgtaca gataccgtca
     2401 cctgaaaatt tccatacact gtaacccacg agataaaggc tgcacacaga gcatgtaaaa
     2461 attgaataca gatttaatgc tctttatgag catattttta ttccctttaa tagcgtgtaa
     2521 taaaacacgc tatttattta cggttggtct tcctgctttc gggctgttct ttctgatatc
     2581 gtcaaacaat atacggcgtt cttctgctga aatcagacgc aacgctgcca gtgccgtcct
     2641 gataacttca gcacggcttg cgccgtactc gttctggaac tcctgcgcta tatccagatc
     2701 agtatctgtc atggtcaaat ttatacgcgt tactgttgat gctgatgccg gtttttgtgc
     2761 ggcagaactt acaaactggc gtaaatccag cgccttaatt tcctgattta ttttcttggg
     2821 gagctttgcc ataaagtttc cttttttata aagtgtgtaa ttatacacac ttatttataa
     2881 atgaaaattt ccgtttttaa tgcgatgtgt aatattacgc actatatgat attcgtttct
     2941 tttaacagta attcaatctc gccacgggct ttgtttcctc gtttgtcgtt gtattcatgt
     3001 acgcccatac cacagttata ggctttttta tgcgcagtgc gcatatagat gcgctgacgt
     3061 ggaacaggaa gccagtcagg atcatcatta agttgattgc gtagttctga cgcttcactg
     3121 tctgacggat tggttgcgca cttattcaga acgatccatg ctttcattga cggattgact
     3181 ccggcctgcg ctgttttaat gtcgaaaacc agccgtccca gcgtgttgat ctcgatgccg
     3241 gatcctggtt ctacgaatgt gaggacgata tcagcacaac tcagtgcttt tacgaatgcc
     3301 ggagatttca taccaggcgt atcgacgaac acataatcaa aacgggcagc cagtttttgc
     3361 accatcgccg gaatatctgt gtagcactcg taatacggta tttccgacag actggcgtta
     3421 gcctgacgtt cctcaatgaa atccttgatg cttccctgtg aatctgtttc caggaaacag
     3481 gctgttttcc cttttttgat gacacacaca cataaggagc aaagcacggt gcttttgcca
     3541 atgccgcctt tatgcccagc aactgtaatg atttttccca tcacaaaatc ctcctggttt
     3601 aatggtgtgt attaatacac aattatttac gtgtggagta tatctggaca attgcgtgct
     3661 ggcaataatt ttatagtgtg tattgataca caataattat ctgtgtagac agaagagttg
     3721 cacccgtagc tcgctggcgc tcacacgcgc agtacagcgc cccgtccagg gaaaccctgg
     3781 acggcgtaaa ctcgctggcg cgagcgctaa ctgcggcatt ctgcggcgca aatcagcgtc
     3841 atcgttgtct gtgagtcagt cgggcttagc aggggtggaa ggtggcccag gttccggttg
     3901 ataacagtag cggcgtagcc gcgcttcggg gcgatgtgac tcctgtgctc ccctggtatt
     3961 ttcgttcgcc ccttacatcc gctggcgcgg ataaaaagcg tgacaatcat caaataattt
     4021 accgattttc aggccagaaa ccttgctaat acatctaaat agatgtatta ttatttcatc
     4081 gaaaggggat tggctccttt ccttcttaag tccaaacgga caggagatta aaaatggaaa
     4141 actacgctaa atttgttgct actgaaatac tgaaacaact cggtggaaat cgtttcatag
     4201 cgatgacagg ggcaacaaaa tttgcttatt tcgatgaaaa tggtgagtgt ggccttttct
     4261 ttcgtttgcc ttcaaacttt gcaatgaagg gcattaattt agttaaaatc aaattaacat
     4321 tttctgatac atacctggtg acatttttcc gtatccgtgg aaccacaaaa aaagaaatat
     4381 caaaatttga taacatttat tgtgatcagc ttgaaagttt gtttagcgaa gaaaccggac
     4441 ttgcaaccag attttaattt taaatatggt ctgattgagt aacggaacaa ttaatacagg
     4501 tgggcaacca gcccacctat cagtaaaaat taatatgagg ataataatat gaaacttaat
     4561 attaatttta cagagaacgg agtacgtggt aacaggggct taattgtccc tttttatccg
     4621 gtgcgctatt tgataaaaca ttaagtgtgt attaatacac atttttaggg cgtgatttct
     4681 ggttgttttt gtaaaagcca agttctatca cgccagactc acattttgtc actttaaaga
     4741 gacattcttc ttgatttgat gtcacttata agagacaatg tgcacaggtt aagggaatgg
     4801 gggcggcaag atgccttact cggcgaaacg atatcagaca gaaaacggtg aggttccata
     4861 caccgactgg atgaaaaaat taagacgtaa agaccagacc gcagcgttaa aggtggattc
     4921 cagaattgcc cgcgctatga gcggaaattt tggagatcat aaattcgaaa gggacggtgt
     4981 ctgggaatta cgagttgatt acggtcctgg ttacagggtt tattattcaa tcgaagatgg
     5041 cgaaataatt cttctgctca ttggagggaa taaaaaaact cagacagtcg atttagataa
     5101 agccgttagt tatctgcaag attttaaaaa gaggtcaaaa aatgacagcc actaaaaatg
     5161 taaagtcgca gaaaaatgat tatccggcat ccgttgatca caatgctgcc atgattgaag
     5221 aattgcgcgc agatcctgat tatgcaaacg cttatcttgc taatgccctg gaagaaataa
     5281 acgagcctgg tggtttaggc ggttttcttg ttgcgctacg tcaggtaatc gaggcgcgag
     5341 gggggatttc cgaagccgcc aggaaatcgg ggcttgcccg tcagagtatt taccgcgcat
     5401 tatccccgaa cgggaacccg accatcacaa cacttgcaca acttacatct gtagccggat
     5461 tacagttcac tctcagtaaa tccagccact aacctctcca gttcccccta agcgggggaa
     5521 tttcctcatg tcagcaaaaa atcatcaaac ccgcagttgt cccgaccgaa gttcactggt
     5581 atttatcgcg gggaataatg ccaccgtacg agtggaaaca tgtgtatttc tgtgtgcccc
     5641 tgtataatcc ggtgatttgt ctgtgactgg aaccttaaag ctaatgtatg catacattag
     5701 ctttaagcct gtattcccgt cgttatcgag gccgcctgga aaacggaata aaccacgtta
     5761 agagtatttt ttagccacat ggcttttggg aatcgccaga atggcgggat aactgtggtt
     5821 ttggtgtctg aaatcgtaca attgtatact ggcaatagtt tattggtgtg tattaataca
     5881 taccttaagt cgatgtgatt tctggcgcgt tgcccccgta gctcgctggc gctcacacgc
     5941 gcagtacagc gccccgtcca gggaaaccct ggacggcgta aactcgctgg cgcgagcgct
     6001 aactgcggca ttctgcggcg caaatcagcg ttatcgttgt ctgtgagtca gtcgggctta
     6061 gcaggggtgg aaggtggccc tggttccggt tgataacagt agcggcgtag ccgcgcttcg
     6121 gggcgatgtg gctcctgtgc taccctggaa ttttcgttcg ccccttacat ccgctggcgc
     6181 ggataaaaag tgtgacaatc atcaaataat ttaccgtttt tcagggcaga agccttgcca
     6241 atacatctaa atagatgtat tattgtttca tcgaaaggag ataggcttct ttccttctta
     6301 agtccaaacg gacaggagat ttaaaatgaa tatccaggaa gcattaaacg tttttggatt
     6361 atccggcgaa ttaactgaaa aagatatcaa agcagcatac agaaaagccg ctttaaaata
     6421 tcatccagat cgtaacccgt taggggctga actgatgaaa gcggtaaatg cagcttttga
     6481 tgtcttgatg gcaaatattg ataaaataaa tcagttccag agcgctgatg aacatgcacg
     6541 atataattac ggtgatgacc tggaaaaagt attaaacgtt ctttctggtt tatctggtct
     6601 ggtatttgaa gtgataggta actgggtatg gattagtgga gaaactatta cacataagga
     6661 aactttaaaa gaaatcgggt gtaaatgggc ggcaaagaaa aaacaatggt tttatcgtcc
     6721 agacgaacat aaaagttact ggaatcgtga agaacacacg atagaagaaa tccgcgcaaa
     6781 atacggtaca accggacagc gcagggcgac agggtggcaa cgcgtggaaa ccagagcgta
     6841 accagaacgg gggcgagaag cccccgatat cataccagcc cgtcttttgg ggcatcgaac
     6901 ccaaaaagac gggaacagca gccacgtagc aaaaagggaa acagatgaac tctttttttg
     6961 aacaatatca ccctgtcttt gaagttgtct gccgtatcct ggggaacggc tggcgcgtga
     7021 acaaacttga tgattgtccg tctcgtataa aactgacgtc accgcagttt aaaaattact
     7081 ctgtgcatat tcggatggaa aaagaccgat tttctgttgt ggggagtgtg gatagtcgtt
     7141 cctggagtag tccgtatcat gtttgcacgt tatccaggaa gcggaatccc gttgatatag
     7201 cagccaatat cgagcgaaaa attttgctaa atgcttcgca ggaggtgtta caggcgattg
     7261 agtatgagaa acgccaggcg gcaaaaaagg acgaaattct gatcctgaaa ggtatgttat
     7321 cgcaacttgt ccagcttgaa agctggtatg gggcattgac aggctttaaa gctgaaaatg
     7381 gattgaacgg taaagtcacc gagcagggcg agcgttatga tttgcagatt agaggtttga
     7441 gtatagatca acttgttaaa attacaggat atttgaaaca gttatgagaa aaaacaaagg
     7501 gcgattgact tattatcttg aagtgattga taaaaaatac cattttgtaa aaaaaataag
     7561 cagttattca aaggagttca ctgacggaaa aacaaaaaga acaaagagaa cgttaagtga
     7621 gctggttttt aatgaaagtg aggtcgaggc aatagacttt acaaaaaatg gtttaagacc
     7681 tgttgataag aatattctct taactatggt gaaagaatat aaggagagtg atgcatgact
     7741 attgaaaata caccggaaaa cataaagaaa ttacgtaaaa aaattggcct tacccagaca
     7801 gagtgcggtg agatttttgg cgtaggctta agtacatggc agaaaaaaga agctaaaacc
     7861 cacaaccagc ttaatttatc gaaaggtgaa tttgagtacc tgttattact tgcaggggag
     7921 caccctgaat acgtcctgca taaaaaagct gaatcccgct caccggagga accgtgagcg
     7981 agaagaaaaa caccacgacg cagaaatcta agatatacag tgtaaaggtt tatttgtctt
     8041 tacattgtaa ttgttctttg tctttacaca gtgtgtgttt gtaacagggc gactttacat
     8101 aattgcgatt ttgcctggtg tgttgttgcg cccgtagctc gctgacgctc acacgcgcag
     8161 tacagcgccc cgccctgctg caatgcacca ggacggcgtg aactcgctgg cgctcgcgct
     8221 aactgcggca ttctgcggcg caaatcagca tgatcatagc ctgttggtct gttctggctt
     8281 tgcagggcga agggatggcc ctgtgcatcc tggtatgttc gtcagcggct gcgccgcgct
     8341 tcggggcgat gtggccccgc tgctccctgg tatttttttt cgccccttct atccgctggc
     8401 gcggattaaa aaatgagcaa accatcaaat aacttgctgt tttcaggcaa taaatcttga
     8461 taatacatct aaatagatgt attattattt catcgaaagg ggattggctc ctttccttct
     8521 taagtccaaa cggacaggag atttaaaatg aaattcactt caaccacgaa tcatgttttc
     8581 acattcgaac gagtaacgct ttgcacgatt gttcttattc ataaggatac gggacaacag
     8641 tatgttgtca tatttacgga taacaataaa attcatgact ataagaccgg aattgtttct
     8701 cagtttggtg aacttaagca gagtgatatt gatttaattc ttttctatcg ggacgaatat
     8761 gaaaaatatt ttgactcgtt aaataatggc gaggagtatt tgagttttaa agaatatatc
     8821 ggatgtataa ggggaaaata aattatgaaa ggtgtgttca ggttgctgga acaacctgaa
     8881 cacgttatcg acaatcaaaa ctgaatgagg tttttaaatg tcaacacgac agatcgtaca
     8941 gcttgctgat tctcttgtca aaggctggga aaccagaatc ccggcaatga cgtttagtga
     9001 gtgggatcaa ttccggtggt ggttaaacta cctgcaaggg tatgaaatgt tttaaataaa
     9061 aaggggcatt tctgcccctt catatattta gacaaaaata ctatatgtaa ctatagtcgt
     9121 ctgcaagtaa taaagggggg gcaggggatg attaatgaag ctaaaaaaat tgctgtggaa
     9181 atgatgtttt ggacagcttg tcctgtggat gatgataatt gttttgatgc tattattaat
     9241 gcatcaaaaa ttgacagaga atttgaagat aaacatgcga tttatcttca tccgatttac
     9301 agggatttat tatcaactga tcaaataaaa agtgctctga aaaagagagc aataaaaata
     9361 atttccttca agtgtgatat taatgaggat tcggcaagaa aaattttaat gcaaaccata
     9421 ttaagagcgg tttaacttct ggtgtgttgc ccccgtagct cgctggcgct cacacgcgca
     9481 gtacagcgcc ccgtccaggg aaaccctgga cggcgtaaac tcgctggcgc gagcgctaac
     9541 tgcggcattc tgcggcgcaa atcagcgtca tcgttgtctg tgagtcagtc gggcctggca
     9601 gggcgggaag gtggcccagg ctccggttga taacagtagc ggcgttgccg cgcttcgggg
     9661 cgatgtggct cctgtgctcc cctggtattt tcgttcgccc cttacatccg ctggcgcgga
     9721 taaaaagcgt gacaatcatc aaataattta cccattttca ggccagaaac cttgctaata
     9781 catctaaata gatgtattat tatttcatcg aaaggtgatt ggctcctttc cttcttaagt
     9841 ccaaacggac aggagattaa aaatgagttc agaagtgatt aagcaaattc agaaaattca
     9901 agatcgcggc attatcatct attcaaagtt tagagcggca gaatttgatc aggatgacgt
     9961 ttacagagag tcatattttc tggtcgtaga gtttaacgag ttaatagcag aaaacatcat
    10021 tcatgacgaa aaactggtag atcaaacggc ttgtattctt catgagctta gaagaatcgc
    10081 tatagaaggg aaataaatat aacaggggct tatggcccct gttattaact gatgcagaaa
    10141 tagtgtctgg taacttatga aggtgaagat gatggatact ttcgatagtg aaaaatttct
    10201 ctcagagctg gatgttttat caaaagagag catgcctggt tgtggtcttg tttttgagct
    10261 ataccaacgt cgtttgagtg ctgcgattga taaagttgtc gccggattgc cggaagaaca
    10321 acacgcccag gcgtttgaac tggctcgcca ggaatttgat tatctttctg cggaagagat
    10381 tgctgaagaa atacgccgtg actcagaacg tggctattgt tgtcacggct ttgatcgcga
    10441 ttgctgccct ctcggttgcg gtgatttacc atgaaatacg ggaaggggct tgtagcccct
    10501 tctcacaagt gctattcaga tctggtcgtc ttcttcttcc tcgcccactc gctcactacc
    10561 gttcggtcgc tggggctgtg gtcagcgttt gtaagttttt ctcctatact gacgacgtgc
    10621 ttcctgatgg gtattattgt gtaataatac acattattac tgtgttaagt cttaaatgtg
    10681 tggttaatgc taatgtatgc atacattagc attaagtttg ttgtgctgaa cgtacaataa
    10741 ttctctatat ccaaactgac cccaaatatg tcgctggaag cggcatttaa ttaagtcagg
    10801 agtttataga gaaaaaatcc ccacattgtg aattgtaaga cagtgctaat aaataatgca
    10861 gcaccacaat aaaatttaaa tgatttcaca ctggatttaa aagcattatt aattgctgta
    10921 tcaatattct tagatatact ttcacctttc ttttcaaggc ttctgatatc attaacataa
    10981 ttagctaagg cttttttatt aatttcagcc aggtttgtat ctatggcaga tattatttta
    11041 tttgctgact ctattgtttc tgttgttttt tcagcaactt ttgtaatgtt taattgaagc
    11101 tcttttgcac tttctgctaa tttctctgtt gcattaattc gggcttcttc cagagaacct
    11161 ttaatttctc ctttgctttc ctgaaattcg tcatgaagtg ctactgctgc atgttgtagt
    11221 tctgacgttt tttcatcaat agcgtcaggt aatgcgttga ttgcatctct tattgaagat
    11281 atgtcatgag ataatctttc agtagctatt tgattactgt ttttaagttg tgttagtgca
    11341 tgttcgagta ttttttcatg cattagtatt atgcttaaaa caggatcgtc cttacttatt
    11401 tttaatctgg tctctttaaa aatatcagaa ataatgtttt cgatattaaa tgatttatca
    11461 ttaatcataa cttgtcctca aatatataac agcccataat ggctgttata taatcactta
    11521 tgcttctgtg tgctgcatgt tatagattga atcaagttgc acatatatat cgttaaaaac
    11581 tcgcttgaga cgagatttag ccattaatcc aaaaaattct gattcaaggg cttcttttaa
    11641 tgttagtgat ttttcggtta aatttttgat gtcagaagcg aaggcatcgc tttttctgtc
    11701 ctgaataacc acaactccag cgatgacatc cttatttttt tcaatgaatt tagttttaat
    11761 taaaggaata ttttcaagtg ctggtacacc ctgaaattca ttaatccata ctattatttt
    11821 tacttttgtt ccttttacta gtttaacaag atcttcaaaa ccttctattg tatctgcttg
    11881 cgcctgaccg ccaacaataa tagtatggat ataaacttct tgttcaactt cgttaaacat
    11941 atccattaca cagttgtcat tgaaatactg aattaatggt aaaaaagtag atgctccatt
    12001 atcaatgaca aatgtatttt gagtattagt tagcattgat tcgaacattg gatcgaattt
    12061 agattggact actttgctgt tttcagtgat ttgtattaaa tcagcattta atctttttac
    12121 tttagctgta gttgtattta caggatctgt gtcgcctaca acgacgttat ccatgttttt
    12181 ttcatcaata aagtattgtg ctagtattgc tgttgtaaat gattttccta caccgccttt
    12241 tccttgaagg ataaaattta ttgagttttt catttatgat acacctttat ttaaagatat
    12301 ttgtttataa atttttcatt tgttgcgctt ggatcgtgat ctatttcgtt tttgttctcg
    12361 ctgattgttg aaaaaaatcc ttttacgcct cctctttgtt tgttgtcttc atctattttt
    12421 ttttcaatca tattggtgct tttttttagg tctttttgcc cagtgcattt ttgccttttt
    12481 ttggccctct cggtcatgtt tatataagtc ttttgggcta gttctgtctt ggttttgtta
    12541 tttatgtaat ctgttatttg ctcccatgta taatagcttt ttgcttcaag tatgttcgat
    12601 aaatacatat tgaagacagc ctgttttgat tttgaatttg tttcttttat cgcctcgcat
    12661 attggagata ataaactatc gatctttttc atttgttata tatcctctct tattacctcc
    12721 tacattggca caatatttta ttcaaatcaa tttgcttttt gtttgtgtgg gtgattatgt
    12781 gggttgtttt gtgggttgtc aacatggttg taggcttttt tgtgggttta agtgtaggtt
    12841 gttttgtggg ttgcttgtgt ttgtgtgtgg gtgattatgt gggttgtttt gtggggctgt
    12901 gattcgtcga gttgatcttc ttttaaaata caggcacgag agcgatgtat acacggaata
    12961 aattccggta taatcgacgt gcttaggggg atctccccct aataccccag tatatctcta
    13021 caaccaagtc gctatatgga ggttctacga accatgaaaa aacaagacaa aggaaaaaag
    13081 gctgtagcct ccacatcaaa ggtggagaga aaacatgtga tatcactgcg attgactgat
    13141 gaggaatatc agccatttca tgagtatttt acatctcttg aagtcagtcg tagtgagttt
    13201 tttcggatgg taatactgaa tcgtctggat gatattccga ctttgaaaca aaaacgtcca
    13261 gttgaatata gagaattact cagacttttt aataagtcaa gcaataatat taatcagata
    13321 gctaaaaaaa ttaacacaga atatcgcaat agttttattt cagaaagtac atatttacgc
    13381 tatcttaatg ttcttgttaa tattcgtgat acgtttgttg cagggattaa taaatgttag
    13441 tcagaatcag cggtggcaat aagggggtat gcgaatacct tgaaaaaggt cataagcaag
    13501 gaagggaata tacccgagat gaaattgacc atcggttagt tctggatggc gatctggcat
    13561 tgactgaatc agttattgac gcgatacctg ataatggtca ggagcgttat atgcatatta
    13621 cgttatcctt ttttgaagat gatgtttctg aatctaagtt gcatgatgta gtccaggaat
    13681 acaagtctct catgatgaac gcttatcatg aagatgaata taatttttat gcggaagcgc
    13741 atcttccaaa aataaaacag atgcctgatc ataaaaccgg agaaatggtt gagcgtaggc
    13801 cccatattca cattgttatt cctgaagtta atcttgtgac agggagtaag ctgaatccaa
    13861 gagggatgac agaacattat atacaatatc ttgatgccat acaggagcac attaataata
    13921 aatacaatct tgtttctcct aaagatggtg tcagagtcag tgaccataac cacgctaatg
    13981 tattagccag ggctaaaggc gatttttacc gtgagcggca cagtgagttt aaaaccacgg
    14041 tttttgatgc gctggataca gagaatatcc gaacagagaa acaattttct gattatcttt
    14101 cccgttttgg tgaggtaaag gagtataata aggggaagtc tgaacgctac tttggtgtga
    14161 agctcgaagg agactctaaa tttactcgac tcaaaagccc tttattttcg cagcagtata
    14221 tatctgatcg tactatacca cttgtaaaac ccacaaagac gcagattgag aaccgtctga
    14281 aagagtggaa gagttcagtc agtcatgaga taaaacatat tcatccggca gggcaaaaaa
    14341 ttcgtaaaga atatgcagca cttgctggtg aagataagca actgtatctg aaaaaggtaa
    14401 ttaatcgtta tgacgaaaaa cacaaccttc aaagacagga cagacgacag aatagtgacc
    14461 agcccggtgt taaatacaat accggaaagt ctgtatccgg taagacaatc ggattgccac
    14521 gtctgccaac acgcgaactg gtttacggac ttcacggaag atcagatggg aaaagcatta
    14581 ataaatctca gttgttattg cagcaaaatg aatacggtca tttatcagag actggacgac
    14641 aagaagtcga tccagatcga tatgtgcgac ggttgcagga tagagcagga agaagagtag
    14701 ttcagtcacc gcttgaatca aagattaaag cagcgattca gcgcaatgat gatttacctt
    14761 ttttaaaaca aattcgtgca gaaattaatg ctgaacgtta tttgtccaat ctgcaaacac
    14821 gttttaacat cgacccatcg aagcatactg ttacctatgc accggatggt tctccgcgtt
    14881 tcagagttgg caatcgaaat atgaatgcca gtgatttttt aactaaacat ctcaatcttg
    14941 aatgggatga agcaaaagag gtattacttg atatctggca ggatcagaaa gataacaaac
    15001 cttttgatgc tgttattacc cgtgtatctc tcactcgtca acaggcaaaa gaaagattta
    15061 tttgtttaaa tgaacataaa ggttgggtaa ataatgagat taaatataag cataatgata
    15121 atatagcgac ttacagggca gaactcaaag ggcttctggc tataaggaac aagcaagaac
    15181 gggaggttgc tagaggttac attatattta aaaaactcga aagaaagcgt gaaataaact
    15241 caattcgaaa agcatgtttt cgcgaaataa actcatacta tcaccgttgg aatccagaca
    15301 ggagatatga tatggcttta ggtgattctt taaaaaagat aattcgtaat ccggttgatg
    15361 gaaacagcat ttcttccggt aatgaaaagg aagttttcag cgtagttaag cgagtacagc
    15421 aatcccagca agttcaggat tacgtaaaac gtgaactaac cgatcttgtt cctcataagg
    15481 tcagtccaaa agaaacgcgt taccttgatc cgaaaacaac acaaactgtt ttcattgata
    15541 aaggaaatca tattcgtgta aatggtgaaa tcacccagga taaagctgaa gctgcgcttc
    15601 tttatgcaaa agagaaattt ggcggaatgc tgaaactcaa tggctcggaa gaatttaagg
    15661 tggcctgtgc aatggcggca gccgagaagg ggatgaatgt tattctgcga cctgaaaaat
    15721 atcatgaaat gatgcaggta cgcctccagg aactgaaagc tgaacatggc catgaaaaca
    15781 gcgtcgaggc agaacgtcag caacaagtag ctggacagaa accggagcag gaaaagagag
    15841 cagagcaggt taattcggtg gagaaagaca cccaggccaa ggaaagagcg gtggaagtgg
    15901 cggaatctgt acgcaatgat gataaatcgc gtgatgcagt gccagaacac cctgttaagt
    15961 cagaaactgt ttctcaaaaa tccacgtctg aaacccccgt ggtacaggaa gcggaaaaac
    16021 aggagtctgt gctgcagaat gtttcggcta agtcagaaga acgtgaacag cagccacaag
    16081 aatcacagca acagaagcct gtaacagaga aagacgtggc tactggtcag gagaaacaga
    16141 ccggaaatga gcaaagcaca gaatcagcgt taaccactga aaatccccag cagcagaata
    16201 acgcatcgct acagcaccta caggaagagg caaaagttat tgccagccag cacaaggaag
    16261 cccagcgttt actgcatgaa gctgtagaaa atggtgcgcc gaaagaagag gttgatcttc
    16321 tttcgaaagg ggttaacagt atttcggctc gtgctcagac gattaatgaa cagattaaag
    16381 cctttgagga acaacaaggg aagaaaaaag ctaaatcagt cgaggaaaaa aacgagaata
    16441 ctacagaaaa gaaaaagcgg actgtcaata gtgaaaaagc gatggaaaga gaaattgagc
    16501 gagtttctaa gcaactggcg aaggaaatga atattgacaa acaaacagtc aaagatacgc
    16561 tcaaattcat acgctttgag gatgtaaacg atctcaaggc agcaatgaaa aaccctgaaa
    16621 cggtagaggc cgtgaagcgt gaaattgtga gttatgacaa cgcattgaaa aatgagcgtg
    16681 agcgggaaag aagcagagat aacactcgta cccgttaatc agaaaccaaa aagcccgact
    16741 ggtaatcggg ctttttggtt ttccggtctt gagttggtgg ctctgaccgg aggaagacac
    16801 tcagtatcca acatccataa tattatggca ttagacacaa actatatagt cttacttttg
    16861 acaacagtat gacttctatc agcaaatggg tataatcatt tcgttaggag tgtgtaattg
    16921 tttgacacta tatttaggat aaattatgga tatatatatt gattataatg tctatttgaa
    16981 cattcttaat aaagaagatg actatgaatg ggttgttgca aagttgaaca atttaagaaa
    17041 atcgacttca tttatagttt actcaccagc acatattgaa gagattgctg aaaatagatc
    17101 tagagataat gttgacattt taaaagtaat gaataaaaca atgcatatca tcagtcgtta
    17161 cacacgcaat gtggaatact tgccagggat aatgactaac gatcaaatta aagatttatt
    17221 atttcaatta tctgataatc ctgcggctat tagtacacgt ttaaacctgg agaaaatgtt
    17281 attcaaaaat ttgatatctg gaatttctga tgatgactgt aaaacacgta gagttattga
    17341 atcaccaaga gattgtatga agagagttta tggtctaata gaccaaactg acgtagccca
    17401 taaaaatgac atataccata taggtagaag gaatgataaa tctttatgtg ataattttac
    17461 tcaattagga atagaatatg atagtattca aacttttgag tcgtatcaaa aggaaaaaaa
    17521 attaggccct aaaagattat caaacattgc tccgcaagat atattcaatg acgagcatat
    17581 aattgaagaa ttcaatataa aattacaaag ctcaggtttt atgaaaaaac ctacacaaga
    17641 acagttatct aatagccatc aactaagaag tgacattatt actctgacct taaatttctt
    17701 ggaacagatt ggttacaagc aagaaagaaa caattcttca tatgtaattc gttcaagaat
    17761 gcatgatgtt tctcatgcaa tttacggagc gaatgcagac tacttcgtaa cttttgacaa
    17821 aagactccga gataagttgg ttgctacata tgaattttta aatatacctt gcaaagttat
    17881 ggataaaaat gaatttatct gtcataactt ttcacatcca gttgtaataa atataggcgc
    17941 gaacgctgtt ccatagaata cagaaggcag aacccgctgg cgtagggctt cggcggataa
    18001 ccagtgaagc catatttaac aacagaatgc cgccttccat gccgctgaaa gcagtatcat
    18061 tgagcgggaa agaagcagag agaactcacg ctctcgttaa tcagaaacaa aaaagcccga
    18121 ctggtaatcg ggcttttttg tttttccggt cttgagttgg tggctctgac cggaggaagt
    18181 tactctacac ctaactttta cattttattc actatgccag tgtaaagcaa ctaaatgtgg
    18241 ttaaggaacc acatttagtt gtgtaacggg ggggttgtgt agagtataat cagtcagtta
    18301 gtcgtgtagt tggcttgcta tcctgctctt cctgtctctg cgggtatgaa cgattaagtc
    18361 ctggctgaat aaacaggtgg tgctgtgttc agtgctctgg caggaggaag tcactctaca
    18421 cctaacaatg tgagagagcc gttgtgacag gcagtgtatg agtaagcagc ggtgatttcc
    18481 tccaggttat ctggagacac ggatgattaa cctgttaatc atcgttctca gagccgtagt
    18541 cgccgttgca aacgcgctga ttgcggttct ggaactgatt cgccagttca ttgattgatg
    18601 acagcggaaa cgtaactaag ggcaggggct aaccacctct gccctttaat aatgatatcc
    18661 gtttttcctt tcaatttttt tgttgcggtc gctgatattc agcgtgttgg ccagctgctc
    18721 gatgccaatg ccggcaatca gatgattttt tatttttcat aaagttagca tctttgttgt
    18781 gacagataat gttgtcaatt tttttgatta atggcgtcta acaatttaaa aataatcgct
    18841 ttaacattat cggtcttcga ccacaaacaa ctgagtggct gtaaattccg tgcggttggg
    18901 cttagcaggg ggcgcgagcg cagttccaga gaatatagaa gccagtaccc gctggcgcgg
    18961 ggcttcggcg gataaccagt aaagccacat tgagctacag agtgccgcct tacatgccgc
    19021 tgaaagcggc atcattgagt gggaaagtaa aggctcatcc ttggttaatc ggaatcttca
    19081 ggtgtgccta aaaaagaata gaatagactc gcagataata tataataaaa aatataataa
    19141 aaagaatatc agaggtgcta tagcatagaa tgctggagtc ataaagtcct tatctatata
    19201 tgattttttt ttcttaacat ggtatctgaa attcactcgt ttacccgttt tcacattaca
    19261 cctaccattg ctttatttat catcgataaa aatgagaaat ctaatgctcg caggacaaaa
    19321 caatacaata catctaaagc ccgtacatta ttaggcttta gatgcatcat attgatttct
    19381 tcttacgggc acaatctaaa acctgacaaa ccacctctgg ttaatcggat gcttcagacg
    19441 tgccattgga tattatatgg agaacaagaa attatcaaca atcgctattt ttgggggaga
    19501 actcaaaccc caactttccg gttgctttat caatcagttt aaccttagcc gaaaattctt
    19561 tattcgtcct ggaactaaca aaccctttta actctcctgt aatacctttt gtcagcaagg
    19621 tttctacttg cttgtcagac agagccttgc cagagaagtt cttccagatt ttaaattcac
    19681 atcctgtaca gaaataacct tttggcctga tagcaatctg cttaccacag tgagggcagg
    19741 gagcattcag gcgttgagat tggcctgctg gcgctgctgc ttcaatcttc atctcgccca
    19801 ggtcaacatc tgtaatcatg ctgctcagtt cgctgtaaag ctcattaata aactcttcaa
    19861 ccgtcagatc accattttca atagcagcct gtttttcagc ccataatgct gtcatatctg
    19921 gattaacagc tatatccggc agagcatcaa taagcgcata tccggtatct gtcggaatga
    19981 gttttccttt ttccagcgtg atataatttc tcttcttcaa cgtttccaga atcgctgcgc
    20041 gggtagctgg cgtaccaatg ccgccatgtt catctttttt gtctttatct ttatccttca
    20101 acaatttttt aatcgttggg tcagtgacaa aatccgcgac acgaacaagc gcagcaagca
    20161 aggaggcttc agtaaataat ggcggcggtg tcgttttctt ctcattaaca acaacttctt
    20221 ttgtcgtcag tgtttctcct gtgcgaattt tacagagcag ttcaaaagcg gaatcatcgt
    20281 tatcttctga ttcaccttcg tctgtggttt ccgcaccaag aaatgcctca aatccgctgt
    20341 cagttgtttt tctggcacga gcatagaacg actcatcacc acactgaatg gcaaccgata
    20401 cttcctggta tgccttttca ggcatgaact gaacaagata atgctgtgcg atcgccagat
    20461 aaacattacg ctcgtcagta ctaagtgaat taatatcagg tacactgacg gttggaatta
    20521 tagctgtatg tgcagttatc tttgcgctgt taaatgcctt actttttcgt gcagaattaa
    20581 tatccaactg ttgagggaat accgctttca gagcatcaat aatctgcggt gcttcacaaa
    20641 attgttcatc tgaaagatac gaacaatcag aacggttata agtaatagct ttgtattttt
    20701 cacgaagctg ttgcgtaata tccagcgtct tttgtgccgt cattttgaac ttcctgttca
    20761 tatactgctg aagacgaacg aggttaaacg gtaagggtgc agccgttttc ttatcatcag
    20821 ttgctgccgc ttcaacggtg gctggtttac ctgcaagaga tgccgcagtt ccgtttgccc
    20881 acgccttatc aagtaattta cggtctgtca gcggggcaaa ttctcctggt ttccagttcg
    20941 ccctgataac atcagcaccg cgttgaaaat atccagtcat tgtgtaaaag acactggatt
    21001 tatgattttt gttggcacgg gtacgattca caatcaggcc aagaacaggt gtctggacac
    21061 gtccaacaga caaaacccct ttatagcctt tattttgtgc tggaatcgtg tacgcacggg
    21121 tcatggagaa accgtagatc tcgtcagcaa cagaacgtgc caacgccttg aggtaaagcc
    21181 ctttgaaatc acgattatct ttcaaatttg ccagtgcctt tttcactgcc ggaagcgtgt
    21241 tgtcgttaat cagaacgcgc tttacgggtt ttgtgttgcc agcatattcc aggacttcat
    21301 caacaagtag ctgtccttcg tcatcaggat cacccgcatg aataatttca gtcacatccg
    21361 cgcgattaat aagttctata atcgttttaa gctgttttga agccctttcc acaggctgat
    21421 atttcacagg ataaaggcgt aaaggaagcg tttcaacctt ccagattttg tattcaggat
    21481 tatagttttc cggcggctgt gattcgataa tatgaccaaa acagttagtc accattgcgt
    21541 tatcactttc aaaccagcca tcatggcgag taaaattgcc accaagcgcc ttaataatat
    21601 catttgctac tgcgggtttt tctgcgataa aaagtctcat tttgttatcc tttacattga
    21661 attaatctgt ttgcacaacg aatagcacgt tcttctgata ctgcctgttg ccagttatct
    21721 tcacttacag gttgtctgaa tctgcgctca cagccattaa aggcaaaaat tccctcacgg
    21781 ttaagtatat tcgttgcgat atcctggtct ttaattctca aaaaggctgt tacagcaaaa
    21841 ccagaatatg aagcaatttt tgaaatgatg cattcatcag cttttattaa tgcggcaaaa
    21901 ggaactatct tttggtgtcc acacgaaatc aaagggaggg tgtgaatatc ataataagct
    21961 attgaatggc aatccatgcg ggtatcatca atagctgcca gccagaatcc tgtctggtct
    22021 tttatttttc tgctaatatc tgaatatgaa acaccaggat ttaataaata tccaccatag
    22081 cagacatgga aggggtgaac agattcatca atatcaggaa gttcttttac cagattttct
    22141 ttccagtcct tcagaacacc aaattcaaga gcatccagca aaaactttcg ttcaaatcca
    22201 tcaaacaatg catattgcat aataaccacc aaagagtatc tgatttatat aactaatgca
    22261 ctttcaatag acggttgtct tttgaataaa gaaaaatctg tattcaatcg ttcagactga
    22321 atagtgtgaa gtaaaaggct ttcttctctg gacagaagga agtcacccca ttcgaactct
    22381 ttccacatat catgactgtt ttcaccaggc ttatttaata aggactccat tttgggctgg
    22441 attctggctg gaggcttatt ttcataagct gaatgcaccc tgtcactcca cttatcaaaa
    22501 gaaccattta aaagaatttc ctgtgacacc atatcaaact cattatcttt tatataagta
    22561 ttaatctgat ggtaaattag ccccatatcc tcaatatccc aggtgtatct ggtagtaaac
    22621 catcttaatt ttttttctgt ttgcaaaata agaccattaa ctgattttct gcctttcagc
    22681 attccaaccc atacacattt attaggccac ggtctgccta cagacaatga ttcgccatct
    22741 attattaaat caagttcagg tatgacgatg gatttaagag tgcatttatt cgccatgcga
    22801 tgaaagagtt tagggacaat atgcaggtac aaatcgattt cctttatttt tagcgtagtc
    22861 aaagaagttc tgatttatta acctcatggt gaatcagttc accattgcgt aagttagttt
    22921 tgtttaccaa aataatgcag gttttcaatt gttcctaaaa cttcaaaact gtcaggtgca
    22981 actttacgcc agtggttttt tgtatgagaa gcataacttc tgtcccagcg aagaatacga
    23041 ttaccatctt ggataataaa aaccatcctg tttttagggg tgcgtaatga tttcgtgact
    23101 tttgctaagt cattatctga aagtagttca atagtgagat tattcagatt aattattctt
    23161 tcctgtccac tatttgatac aacacgtatt ttcattgtta attcctttaa ttaactaaat
    23221 tacatgtatt tatcatcatc gtctccattg tcatattctg gaaacactat tagcaatggt
    23281 tcaccgttgg tgcatttcat atgatccgtt attaatctgc caatctcttc atttgtcata
    23341 taacgttcta tttccatttg tttataattg cacataattc aaaaagttcc gtaccaccac
    23401 ccatcatctt tttcttttag ttcaataata atttgatctt catgaagatt tttataaaac
    23461 atatgttccc agactttact gagagaaaat gttccacgac tcatcttaag gccttttgct
    23521 gttgaaatgt ttgttgtttt tattcttatt gttttatgtt ctgggtctaa ttctataata
    23581 acaaatatac catcgcctcg tacctttttc gaaaggtagc aacttacaat gccttttctg
    23641 ttcttgtctt ttcgtagtgt aagtcgtgac gaatgctttc gttcaatgaa ctcacgttgt
    23701 ctgttaattc taataaatgt cattatatat cctttgttta tgattaatat ctctcaatat
    23761 cactatttcg aaaatatcag actaagcaga gaaagaacgg ccataacgtt gcataatcat
    23821 atttaagatt gtttgattag caggtgaacc tgaacaggta taaagaaact tctgtcttgc
    23881 tacgtaggtt gctccgggat tataaaaagg agtgtaggcg cggatgctaa agaatcgctg
    23941 atttatcggg atacaatcag gttgtggtgc tccctgtact ttttcagcca tacagataag
    24001 cacttcacag ggatcacccg ctgttgaaag tccttttatt ttggcactgg taggaagtat
    24061 gaaagcgagg gtggaaagca gtgtgattga tatgaataaa cgaggtttca ttttgatctc
    24121 cgtgaaaagc agaattgctt tgatattgtc agtcttcgac cacaaacaat tgagttgctg
    24181 ttaattccgt gcggtggggc tttgcagggg cgcgagcgct gtctctcaga atattgtatt
    24241 cagtacccgc tggcgcgggg cttcggcgga taagccgcat agccatattg agctacagag
    24301 tgccgccttc catgccgctt aaagctgcat cacggttaat caccactatc accatcagaa
    24361 aaatattcct tctcatcatc aaaatcatct ttatcaaaag cgggttgtgc agctttccat
    24421 gcttcctccc tttcccgctg gagcattaac tgacgaacca cttcatcctg tggaatatca
    24481 agcacaggtg tgtctatgat attttttcgg gaatattttc ttgcgttaac aaaaaaaggc
    24541 tcattttttt gatcgaaact gacaatttta tgtgcaataa atgggcgaca aaactcactc
    24601 attacgattt gtggtaaaga aaaacttgtt ttctttcctt tgactttaaa tttctgatcc
    24661 cgtaagtcag taatttcttg cggtagcata accgcccttt tttctattat tttatttcgg
    24721 gttgtactag ggtttttgcc tgttgtacgg cttatttgag tgcggacaaa atcataatat
    24781 ccgattgttt ctgacacatc ttttacactt tcatccactt ttttaggtgg atagaaaatt
    24841 tcaaccgcac agtttttcag aatggtgttc ccacccatct ttccgtaacc atgtttttca
    24901 tcaaataact gttccctgtt ttgcagaata aacacaaaac gcaggttata acccgccgtg
    24961 aatgccaggg aaacctgaac cacgtcgagg cggcccattg aagtgaactc gtccagcatg
    25021 agtgtgcact gatatttcag tgttgcgtca tgctctggca gagtacgcac gttctcatta
    25081 agcagttgtg aaaaaaacag attggtcaga cgttgatatt gttttaatgc gccgggagaa
    25141 atgccgtagt agatagtcat ttttttacgg cgcacatcac gcatatcaaa atcgttatct
    25201 tcagtggccg cagcaacgac agggttggca aacatcgaca tggtgcggtt aaagtcgagc
    25261 atgatgcttt cctgttgttt gggttctttg gctgcgaact gacggaacag gcggatagta
    25321 tcgtcgctta atggatatgt ggagggcgct ttgcccagtt tttcttcttc cggtaaagca
    25381 tggtattctt tccagcgatt aatgagaact tcattacctc ggcttctgat ctcgttcaac
    25441 atccattttg ccagatctga tccatcagtc ggaatactca gtttcattac cgagttcatg
    25501 ttgactttaa atttaccttc gagttcggta tcgagtaacc acagcaccag actgataaag
    25561 acgtttttac tggattccac ccaggtgtcg ttttcacccc cagtcatcgg aaacaggatc
    25621 actgcgattt tttccaggtc gccaaaacgg tatacaggat cgcgacgaat ataatacaga
    25681 ggattgtagc ggtgcgttgt cagtactgtc tccccgccga tttctcgtgc gtagccgtca
    25741 gggctgaaga gatagacttc ctgttttaac acatttttcc gatatccagc cgtctccaga
    25801 aaattttcta gtttgatatc taggaatacc atcgattcgg tataccagat gccgttaggt
    25861 ataacgatcg cgacaccttt accacttcgt gttggtgcgt acagcatgag aaactgctgc
    25921 ccgctgtata gcagataacg tgatttaaaa cgccctttat tttccggcat ttttcctatg
    25981 agcagcgatg ggtgtttcag atttttcaat tcctcttcgt cggggaaaaa accgctgtct
    26041 gccaggtctc gatcgttggc aagtctggcg ttaccgtgca ccgttttttt ctcctgaaca
    26101 gcgacaaaca tgacgactgt cagaacaagt gcaatcagga atggaagcag agtccctgca
    26161 agtccggcaa acaggtattt tttgagggaa ggttgccagc cgtaataggc cagcgtggag
    26221 aagtacgtat tccattgcag gttgtcagtc ggcagtgaag cgattttcac cagtataaaa
    26281 cttcccagcc agccggaggc tgtcagggcc atcagccaga ctataagcac aggtagaaca
    26341 agtttctttt tcttcatcag tttttcctgt tgtcgtgagg attttcaccg gagagttgcg
    26401 cccgtagctc gctgacgctc acacgcgcag tacagcgccc cgtccaggga aaccctggac
    26461 ggcgtaaact cgctggcgcg agcgctaact gcggcattct gcggcgcaaa tcagcgtcat
    26521 cgttgtctgt gagtcagtcg ggcttagcag ggcgggaggg tatccctggt tccggttgat
    26581 aacagtagcg gcgttgccgc gcttcggggc gatgtggctc ttgtgctacc ctggtatttt
    26641 cgttcgcccc ttctgtctgc tgacgcagac gaaaacatca tacggccagt agttgatttt
    26701 tctctattgg gttgtagtag atctccgtca tatgtgtgtg tttgaagaac accacaatat
    26761 cgatggaggt tttgatggtt ttcagtatga agctgtggtc tagggtaagt cccaccgtgg
    26821 actgttttac aatcgatgca agacgggaaa ttgcgtccag agcactgttg gcgtgaatgg
    26881 ttgtcacact gccttcgtgt cctgtattca gtgcctctag gtaactccac gcctcatcgc
    26941 ctcgtaattc cgccagaaaa atatgatccg gcttcatacg cattgctgct tcaatcaggt
    27001 atcggggctg tacgccccct tgcttataga acagatggac gtgattacga tggtttggca
    27061 gtgccatctc gtgcacatct tcaatggtga taatacggcg atcatgggga aacaggtctg
    27121 caatggtttt tgcaatggtg gttttccctg atccagtgcc gcctacaacc agaatattca
    27181 ttctggccgc caccgctgct tctaaaaaac tgatccaggt tctggggttg cacctggtgt
    27241 tatcgagaag gtcaagcagt ttttgttgtg aagcgctcag ggtgctcagg tttgcctgat
    27301 taacaatgtg gacattgctg aagcggttgc tgctaacgta atcctccagt gtgaagcgct
    27361 tcagtgaggg tttacgtatg gtgaatgaaa tacatctctc ttcacaggcc ggcggcatga
    27421 tgatctgcat acgctcaccg ccaggcataa ccagcgattt gatagggtgt agagcatcaa
    27481 ggggttctgc gctgttgttg tatttgttaa tcgcaatcga gagatcaagc aggtcactgt
    27541 atgaggcccc tggcgcttca tggtattccc agccgttaac gccttcggta ataatttcaa
    27601 acgggcggtt tactataatt tccgtaagcc cctcagcctg aagaaagggg cgtacgcctg
    27661 tactttcaag aaacatgttg attgtttcca tcagcggacc ttatatacag aagaaaaatc
    27721 aatgttgcgt ggcacaatga cagtcagcat ttcgccctga tttacataac ctgttggcgg
    27781 aatgttgatt gaattatcaa gcgtggtttt tgccagttcg ctggatgttc cagtggtatt
    27841 gttcattgtg aagtcgctgt tgttttcgcg ggaaagacgc gatgtaccag ctgcgagtga
    27901 gtcttcaatg agggaaagca ttaatgcgcc cccaaagcgc tcccagaaat gcgtatccac
    27961 ccatgccgga agccctgacg cgcctaggct gtcagcaccg agggaatcaa tacgtactct
    28021 gttattcttg tccttaacaa ttgtccagtt gacgaacacc cgcgccatac cttgtgtgat
    28081 tgcctgtgtt tgttccccct ggagttgtgc gccagcacgg attaatacag ttttcccgtc
    28141 atccgagatc acatctttgg ttaactgaca gagtgttaca ccaggatacg tggtgacaat
    28201 tttggttttc aggacgcatg gaaaagttgt tccagccgaa agcagaaagc gacgattttt
    28261 tatgcgggat acagaaccat cagcaaagtc ggccccctgt aagaaatcac cgttgtctga
    28321 agttgccgac gtagcacctg ccgttttctg gccggaacta ccaggctgtg attgtgagat
    28381 ccgattgtca tcgaccatta cacttcccat cagacgacgc tgtgctggtg tgggtggggc
    28441 atcgccatta ctttgtgcat atcccgcatc agtttcgttt tctgttggca gagtgacccc
    28501 tttattttct ggacgcgttt cttccctgac ttgttcctct tctgaattaa cgttgttttt
    28561 gacttcctct atgcttctgg cgacgctttc tttcaggtca tcaagatttt ttgcctgggt
    28621 attttgtgca tagacctctg cctgtgcttt tgtggtcgca gtttcggtag tttcttcctc
    28681 tcccgaggct gaaaacacct ttgataaaac ggctgtcaga atgactacgg taacaaaaga
    28741 aaaacctgca agtataagga ccgcttttgc ctttttgttt ccctgttttt taccggagtg
    28801 gatgaaattc cggttttgag cgtcggtatc cgcctcctgc gtgtcaattt catcgagttt
    28861 ttcaatcaca atatcgttgt tatttttcat cgttattaat ctccactctg gtttcgccag
    28921 tcagtgttcc gctggcgttc caggttcgcg gtatatgacg gtttgttctg acttccatca
    28981 cctcacctcc gagacgcagc acaaaagctg gcgaaatttc gtgcacaacc atgatgtttt
    29041 tatccatatg gtaattaacc agttgctcct taccgtccgg taagatcctg aacacaaccg
    29101 gaaggtcagc tgcgccagtc cagcggaaac aggtcagacg accgttatcc cagatggctt
    29161 caggtttaat tttcctgcta ccacgcacct cataacgacc gtttacaata ttcccttcag
    29221 tacagggcgc gacaacacga ctccggcccg tcatgacatt gggtttcacg ggttccggcg
    29281 gtttcggata gatgtatcgc aataaatagg tcgggttctg gtcggtactg actaactgga
    29341 tggagtagat ccgtttgttt gtcacaattg cgatattggt atcaggttct gctgctttgg
    29401 gacgcagaaa aatagtgtta cccctgacag ccggatccca ggcttcagga tcaccaatag
    29461 caagaccggt atcttcgctc ttgatgattt ctccctcttc aagctcgata agggttactt
    29521 ctcccgcttt tgttttgaca atggttacgt cattggggtt atagacgatt tgttgtacgc
    29581 gtctgtcaaa acctgcggac gctggcgttt ttgcagcatg acaggtcatg gtgataccgg
    29641 atatgaccag ggataaaagg agttttcgca tcatcatttc accacctctg gatcaacacg
    29701 gtatgagagc acatccagcc cgaacgggtt tatctggcgc tcagcttcag tcgcgatctt
    29761 gtcttttagc catacaaatt tgatggtggc aatccattgt gttggtgtgt atccaggtgc
    29821 gggtttgcca tcgggatcgg tgattgcttt aacaaaacgt ccttgtaccg tgttttcatc
    29881 cagaaaggtg atggactgca cgcgaacaag aattttgtag cttttcttca gcttatacag
    29941 cggagataac gggccgtgaa tcatgttgtt gtattcggcg aatacctggg aggaagacat
    30001 cagttcaacg gtattcagca tgttctgaat tgtgtaccat tcgtaacctt cgcggttctc
    30061 cagatagcgc gtgaggaaat agcggcttac cgcctcgtca tatgtaccgg aatcctgatt
    30121 gaacggggtg atcacatccg tgtatcccgt tgttttgtct acccgtaaca gaaacggttc
    30181 tgttgttttc agcggggcca tgactgcgag ggcaacagcc attgcagccg cagaagcgga
    30241 cgaaaagaaa agcgcaaaac gcgtgatccg cagtgagcgc ttcagttcgt tatcacgatc
    30301 acgcgcaaat tcttttgctg cgtccagtac gtttttttca atttttttat cggtttcttt
    30361 ttgagcgggc gttttacgtg cttccgcttt ggatctttta aaatattctt tcaaacccat
    30421 tatttatatc ccttcataat attatcgacg ctggtgtata atgactccca ttttcctgta
    30481 gcttcaggtg cttcaggggg atttgttgcg gtacatgcag aaagaaataa gcagacaact
    30541 gccggaatga tatatttata cataaaacat accttttaag ccggataaat aagatatatt
    30601 gattgagtaa agagggggat ttatccggcc ttgatgtttc caccaaagcg ggaagataaa
    30661 taatcttttg ctgctacggc taattctctg ttgcgatccc gtgcattctg tgctgttgga
    30721 ttaaagccaa gcccaagctg atttcgtgta ctgttaccag cgccacgcag agcttttata
    30781 acatctccgg ttgcgctggt cagaccgtta atacccacac cgccagttaa ggtacttatc
    30841 agcgttgata tttgagcaag gaggaatttg ccaatcatga atacaaccgc aacacaaagg
    30901 acgctccaga tatttttagt aatatcatcg ctgtttgtat taatatattt attaatatac
    30961 tgaatcagaa ccttgaacag acaggcatag gcaatgttga gaaatatata gttaaaacac
    31021 gaacccaccc acgctgtaaa catattgcgc gtggtgggcc acgcagaaaa caagataaac
    31081 acaccgccaa tggaaagcag tatgccgacc atcatttcgg ccattaataa ataggccgcg
    31141 cagtaaataa caaatccgcc gccagcaaga gcaagaagaa tggcttttaa aataaaaatt
    31201 atccatgctc ccattgaatc aaaccagccc tctgtatctt taaaaacctg aaattgttgg
    31261 atccctaaat ctatcagacg gtttagaagg ccatcgataa cggctcctgt actggctcca
    31321 tcctgaccaa taatcctgga agccacttcc tgaccgcttc cctgtacgaa aggaacaata
    31381 taactaaggt acatacctcc ggcggtactc atggcaaaaa caagcgtcag aacgcccata
    31441 cgttttacgg attccatcat cacatcatcc ttgcgtgtgt acatgatttc ccacgcaaag
    31501 aaaacaccgt agagaatgac ggcggctccg aacaacggag tcacaagttg cataatagtc
    31561 tgaaaatttg tggttacgct gttctgaata atcgaagata attcattgaa catcggctgg
    31621 acgatttcga aattcgcttt tgcaaaagcg gagtcatttg aagccatcgt tattacctgt
    31681 aaaagaaaac gcccgtcaca gggacaggcg ttgtgggtta atcagagagc attcagtttg
    31741 tacggatata cagatcgttt tcatcatctc tgaactgaag cgtgtttcta tcaatcaccc
    31801 gaataaaacg tttttctttt tctcctccaa tccagtcacc atccctaaca agaaaagtgt
    31861 ttgtgttaga attagagtat tccccatttg gagaaaattt ccggtatatt tcttgagagt
    31921 aataattatc cttgttatcg tatttgataa tccattttac aggagcgccg ccactggcag
    31981 gatttttttc tatatatgtt ccggctaatt caccattatc accaccgcaa ccggataata
    32041 aaaaaacaaa ggcaaggata aaaccgccaa gtagtttttt gaacatgatc tacctcctga
    32101 tagtgtatgt tgaattaaaa atgctatcaa acattacagg gaaattgtat atgttagaag
    32161 aggatttgtg attttcttat cagttaaagg aaaagttatc ttctgtatat ttcatcagtg
    32221 ccgcttcctc acgtaattta cgttcacggg ccatacgatt gatttctgcc tcattctgtg
    32281 caatttttaa ctgcatggcg gcagtttcga gaagaatatt attctgaagc tcttcgcgtt
    32341 tttgtggcgt atcggcttta ctgaaagcct tttgagttgc ctttatcttt tctgcgcttt
    32401 tctccaggtc cttactcatc gcttcgtatt tcgtgatttc ctcaaacttt gattcgtact
    32461 ggcgtttgat ccggtcgtac tcttttgtat caaagccgta tttagtagcc atactgcgaa
    32521 tatcagcatc ggtaagttcc ttcttcatat cttcccagaa gtctttcatg taggattcag
    32581 cactgccaaa aatggcggaa taatcggtta taccttccag tcgtcttttg gtatccagca
    32641 cttcctgctt ataggcttca agctggctgg ttgccagatc aacctgtttt ttcaattcag
    32701 caatctgatc ggcagccttc ttaattactt cgacggtttt agctatgctg ccagggtctt
    32761 taacgatgac accgcctaca gaacttgcca cagaactgta tcctgtcagg gtggccccca
    32821 gaaacagggc tgtcagagtc tttttcattg aattttccta tcgctttaag attgtcagtc
    32881 ttcgaccaga aacagccacg tggctgttgg tttcgtgtgg ttttggctta gcagggggcg
    32941 cgagcgcagt tccagagaat atagaagaca gtacccgctg gcgcggggct tcggcggata
    33001 acccgcaaag ccatatttaa gtacagagtg ccgccttaca tgccgcttgt gcggcatata
    33061 tcgggctaca gagacagtga tttttccggc aatttgtctt tgagtcgttt catcatgaag
    33121 ggcagccaga cttcagggat atcgctgtta gcttcccgta taacgtcatc tatctgataa
    33181 atcccctcga taaacggggg ataccagagt tcaggatcgt cagagcctat ttcctccctg
    33241 attttttcgc acaatttaat ccctttcatt gagcaggaaa taacaggcag aaaatcgtca
    33301 aatcccacta ggtccatttt ggcaaagaca cttgagccac tttgcttaat cagcattgtt
    33361 cggctttcga gtttcagctt tttaagctga cgaaattctt tttctgtcag cccaatctct
    33421 ttatagccgc tgtatttggc atcagggtta gggagcagga tttttgtcgg tgtctgctga
    33481 acgagagctg cgaaaatttg acagttaatc gcgtcttccg gtgactgact ggtgagccac
    33541 atcatttccc ccttcatacg accagccttg agcgaagcct tgattaaggc ctgtgtcatt
    33601 gggtagttac agggtttcca gaactcttca acgatggaga gcatgagctt accgcctttc
    33661 tgcattcgtt ttttgtagaa aaacagaaca gaaagaatga cttcacacgc aggatgatct
    33721 cgttgattaa ctgtttccag gattacagtc gtgtcaaaac cgattttgct gtattgatcg
    33781 ggatcgaatt tattttcttc gctgtcagta gcccaggcaa gagcgccatt atcccaccat
    33841 ttagacagac ggacgttcag ttcggggctt tgtatacggg attgcagacg agcgaaacgt
    33901 cgctggcgac gttcaagttt catcaccatt tcaacggcgc ggtcaatctc ttcagcctca
    33961 ctgtcgctac agtcgctgcc atcgttatta acagcacaac gtttgaccca ctctttcagg
    34021 aatgccatca gttcaggatc gtcattgtct ccgatctgga aggggttcag gccggaatac
    34081 acaccctcag acagagagaa atactgacca ccgaacattc tgattgccag ttcggtagag
    34141 cggttgaagt caatcacgaa catatctggg ttaaaacgct ggataaacgc ggcggccatc
    34201 atttcaaacg tggttttccc cgcaccagtc gcacccagca gcattccgtg ccccgcgatc
    34261 atctgtcctg ttacattacg ccctggtgcg ctgtagtgag tgttgaacca gtaaagacca
    34321 tcagtgacgg ttttcagcgg cattatggca gagccatcac caattggatt tcctgatttt
    34381 ttgccatagc tgtgattatg aagcggcatc aggcagcaga cattagtgag cgtgcgttgt
    34441 gtggcaaggg ggcgcagttt tgaggctggc agatgactga accatgcata aggtgcttca
    34501 gacgctgcac ggacaaaacg aaatccccgc ccactggtga caaattccgc cgcgacttca
    34561 gaaccgttat cactggcttc atctggtgtg tttccgaaga cggaaaggac gctgtgccag
    34621 gagccaaaaa ctgtttcctg gttgatgata gcttcctggg ccatatggag ttcggtgtgt
    34681 tgcgtcttac cagcgtcctt tacggaagtg agtttgttaa gctgttgctc aattttcttg
    34741 ctggattttg atagcgaatc gggaataaag gattgcgtca ggataaactc aaatggcagg
    34801 gataacagaa aattccactg accaatctgc gagcctcggg gtacatcttt tacgatataa
    34861 ttggaacaaa atttattatc gtgtcctgtg cgatttttta tttcaagagt atcaaaactg
    34921 aaatagaatt tgctgttctt aatactctct gaaatcttcg ttgcagataa cggtattttc
    34981 tcaaaatcat gattgagtaa atagttcaga tagtttgaac aaatatcgga aataccaccg
    35041 tcactgatcc caagcgtata aatattgtag tctttaaaac ctgtttcaat ctgccggata
    35101 atatctttag cttttttttc gccagcatcg atatcatcat aaggaatgcc tattgtgaga
    35161 aaataatctg ttctgaatga tctttctccg ttaaaagtat cacagtagaa atcacctaag
    35221 cgctgaagaa aaggattgct aggaaaatta tatctagcat ccatagatga ttcgcgtttt
    35281 acaagatgtt tccacagaaa aactctttca cttttacaga gtccaagaag tgtttctttt
    35341 tgtccgttaa agttgttcag aatcacatca tctgtcaggc tttcataggg aatgccttca
    35401 ataagaaaag tgagcagtag ttttttatct tcaagccaga tgatattttc atgaatatgt
    35461 gagtgataag gaggaatgaa attattggtt attttcttta tcgacatatt attcaacctt
    35521 cagaacgtta ttcgaacgaa atcccttaag gcacagacct ttgaatttta gtgtcctggc
    35581 agacatggcg ttcgggtcat cggcacacat ttgtttgatg gcgatcatta cgccgataat
    35641 aatggaggga ataataaggc cgtaaaaacc aatagtcagt atgccaacaa aaaaagaaac
    35701 catgagagcc agcagacaca gaataaaagg aatgatgggc atacctttga tcattgcagg
    35761 acgattcagg ccattgtagg taagaaagat tgccttaagc tcttcggatg ctttaatttc
    35821 ttctttatcc aacttttgat cctcatgatg aaccggaata catcatgtat tccggtgatt
    35881 aggtaattat ttgaaaatct ggtagcaggc ttcagcagcc accacacaac caccggcaat
    35941 tgcggcatac atcagtgatt tgagaacgcc agaccaggag tccatttccg tgtatgcctg
    36001 gaatactttc cagagaatgt gtaaaatagc tatagcacca acaaaaccat acgcccagat
    36061 acgcagttca ccgatgaagt ttgtgccttt atcaagccca ccaacacccg ctgccagagc
    36121 cggatttgta gcaataaagc tacaaagtaa aaataaaaag aacgttctgt ataatgcaat
    36181 agatttattc acgattttct ccatacataa ttaggtaaca ttaattcgat tgtggttaaa
    36241 gatggtagga ataattaaat taatggctgt gtgttactcc ttaaaatcct gtattccgaa
    36301 atcaccaaaa acatcccatt tatcatggtt atcactggat accattaaat ggctggatgt
    36361 ttctgaatgt gactttattg ccggaatatc ataatgagta tgtattccgg ctttttcctc
    36421 aattatatcg atataacttt tgccattgct ttccttcaca taaccccgtt tgttattacc
    36481 ggagtaataa caacttatag cgtcacgtag tatcttttgt tggccccccc cttttgtatc
    36541 gtatttatcc cagcacgttt tcagcactgc cgcagcagca cgaatattcg cacagggaga
    36601 aaaaacggat tcatgagtta aatttaaagt ctggaagtta cggctgttta tctgacctag
    36661 gccagcactg aaattttttt tatctgtagt cagttttttt gcgtgattaa cagcctcctc
    36721 ttcagtggta aaatatttac tgccaccgtc ggtgacattt gctatagcgt agggattccc
    36781 accggattca gtcataataa tggcacgcat agtttcagga gaaacaccag gtgcgcactg
    36841 actgatcagt gtcataagca cagttgcgga tatagacatt ttttgacagg taacgatgaa
    36901 agtttaattt aacggattat tggcagcgtc agattactta gggatgttac ttctggttaa
    36961 atgatatgtt gcgctccttc gtgagcagat atcaattatt tctcgctcat catgaatcgc
    37021 cagtttttta ccggataaca gtctgttaaa tagttcctgt tgcacatctg gataatggct
    37081 ggtaagaaca atcagaaatt gtattgtttc gcggtctatt attacgtgat cacttaaagt
    37141 attggggggg gaaataacgg atagaatcaa tagcagcgat tatataggga attaaaccca
    37201 aagaaaaacc gactgtaaat atataaaaga tattttctct attaaggaaa acgctaaata
    37261 gaccgaatgc tgtaatgatt aatagaatca caagacgtat taatctctgg atcttccata
    37321 atttgtggtg ttcatcaatg atgctgtgaa tcgtgttaac atcatgggga attgactcaa
    37381 gagttgaatc ataccatttc ggaataaaat ttttatgctt catgccattt tcctcaaata
    37441 aaacaggggc attgcgcagt agaataactt tctaataata tcgcttcagc agtgtcggtc
    37501 ttcgatcaga aacaaccgtg tagctgttgg ttccgtgcgt tttgggctta gcagggggcg
    37561 cgagcgcagt tccagagaat atagaagaca gtacccgctg gcgcggggct tcggcggata
    37621 acccgcaaag ccatatttaa gtacagagtg ccgccttaca tgccgctggc gcggcatctc
    37681 acgaccaacg gaaacgtccg gctgcgagcg tttttcggac ccctttgatt tctctgttga
    37741 gtgattcctc agaacggtca atcaggccgg attttgccag gccataaata acaagcagta
    37801 tgtcatgtgt aagtagcaac tctgctctgt atgcaggccg ttcacgatat atccagggag
    37861 aacttaaggc tttttcaaca taatacagac tggatatttt ttgttttggt ggaatataac
    37921 agcgatgacg taatttcttc aggtttgctt ttgcttgtga cataacatgt taatctgttt
    37981 catcatttta ttgtaacatt taaatttttt tcaatttttt cacagtgtga atcggcccat
    38041 tcataaaatg cagtgttttt ttctgtctca gcttcgttgt tcaaatcact cacaattaac
    38101 ataatcagtc ccgcaaaaga aattgcaaca agacctccgg caattaatcc atcaagataa
    38161 ttactatctc tcattatcat tccctgtatt tgaatgtgtt tttcacgcac agttaattcc
    38221 cctttgcagg gaatcgattt tgataaaggg aattaactat gttgaaaatt gtaaaaatga
    38281 gtcgttacct gtcagatggc gcggcacgtc actctatacg tagcccacac tgaacactgt
    38341 cagcaagtgc tgttttttgc acacaattta tcctcctcat gccagggctt caggcaatta
    38401 ccgctgtcca ggggtgttaa agagcgtacg aataacgcct tgttcgggcg tttggttgat
    38461 agcggaaaac ttcaacatgg agactttaaa tcagtacaat agatcgttca aggtggttta
    38521 tacgatctat tatcccgtac aaagcaaaat aaaaagtata atattctgaa atcagctttt
    38581 gtattggtat gatagatcgt tgaatagatc attcacgatc taatattcta aaaattgaag
    38641 aagtggc
//
LOCUS       DAQSWQ010000032        38572 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.032, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000032 DAQSWQ010000000
VERSION     DAQSWQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.032"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="R8G18_001681"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="R8G18_001681"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HEE9795402.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="R8G18_001682"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="R8G18_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HEE9795403.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="R8G18_001683"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="R8G18_001683"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEE9795404.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="R8G18_001684"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="R8G18_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HEE9795405.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3342..3545)
                     /locus_tag="R8G18_001685"
     CDS             complement(3342..3545)
                     /locus_tag="R8G18_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795406.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3623..4717
                     /gene="ddlA"
                     /locus_tag="R8G18_001686"
     CDS             3623..4717
                     /gene="ddlA"
                     /locus_tag="R8G18_001686"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEE9795407.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(4741..4953)
                     /locus_tag="R8G18_001687"
     CDS             complement(4741..4953)
                     /locus_tag="R8G18_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HEE9795408.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5213..5521
                     /locus_tag="R8G18_001688"
     CDS             5213..5521
                     /locus_tag="R8G18_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HEE9795409.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="R8G18_001689"
     CDS             complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="R8G18_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HEE9795410.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="R8G18_001690"
     CDS             complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="R8G18_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HEE9795411.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8259..9416
                     /gene="ampH"
                     /locus_tag="R8G18_001691"
     CDS             8259..9416
                     /gene="ampH"
                     /locus_tag="R8G18_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HEE9795412.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="R8G18_001692"
     CDS             complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="R8G18_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HEE9795413.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10128..13016)
                     /locus_tag="R8G18_001693"
     CDS             complement(10128..13016)
                     /locus_tag="R8G18_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEE9795414.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13541..14515
                     /gene="hemB"
                     /locus_tag="R8G18_001694"
     CDS             13541..14515
                     /gene="hemB"
                     /locus_tag="R8G18_001694"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEE9795415.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14621..15472)
                     /gene="tauD"
                     /locus_tag="R8G18_001695"
     CDS             complement(14621..15472)
                     /gene="tauD"
                     /locus_tag="R8G18_001695"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HEE9795416.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15469..16296)
                     /gene="tauC"
                     /locus_tag="R8G18_001696"
     CDS             complement(15469..16296)
                     /gene="tauC"
                     /locus_tag="R8G18_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HEE9795417.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16293..17060)
                     /gene="tauB"
                     /locus_tag="R8G18_001697"
     CDS             complement(16293..17060)
                     /gene="tauB"
                     /locus_tag="R8G18_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HEE9795418.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17073..18035)
                     /gene="tauA"
                     /locus_tag="R8G18_001698"
     CDS             complement(17073..18035)
                     /gene="tauA"
                     /locus_tag="R8G18_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEE9795419.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            18645..19316
                     /locus_tag="R8G18_001699"
     CDS             18645..19316
                     /locus_tag="R8G18_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEE9795420.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            19326..20522
                     /locus_tag="R8G18_001700"
     CDS             19326..20522
                     /locus_tag="R8G18_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEE9795421.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            20522..21082
                     /locus_tag="R8G18_001701"
     CDS             20522..21082
                     /locus_tag="R8G18_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEE9795422.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            21084..21857
                     /locus_tag="R8G18_001702"
     CDS             21084..21857
                     /locus_tag="R8G18_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEE9795423.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            22045..22320
                     /gene="frmR"
                     /locus_tag="R8G18_001703"
     CDS             22045..22320
                     /gene="frmR"
                     /locus_tag="R8G18_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HEE9795424.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            22355..23464
                     /gene="frmA"
                     /locus_tag="R8G18_001704"
     CDS             22355..23464
                     /gene="frmA"
                     /locus_tag="R8G18_001704"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HEE9795425.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            23557..24390
                     /gene="fghA"
                     /locus_tag="R8G18_001705"
     CDS             23557..24390
                     /gene="fghA"
                     /locus_tag="R8G18_001705"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEE9795426.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(24616..25155)
                     /locus_tag="R8G18_001706"
     CDS             complement(24616..25155)
                     /locus_tag="R8G18_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HEE9795427.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(25257..26468)
                     /gene="mhpT"
                     /locus_tag="R8G18_001707"
     CDS             complement(25257..26468)
                     /gene="mhpT"
                     /locus_tag="R8G18_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HEE9795428.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQLSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(26755..27768)
                     /gene="dmpG"
                     /locus_tag="R8G18_001708"
     CDS             complement(26755..27768)
                     /gene="dmpG"
                     /locus_tag="R8G18_001708"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEE9795429.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(27765..28715)
                     /gene="mhpF"
                     /locus_tag="R8G18_001709"
     CDS             complement(27765..28715)
                     /gene="mhpF"
                     /locus_tag="R8G18_001709"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HEE9795430.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(28712..29521)
                     /gene="mhpD"
                     /locus_tag="R8G18_001710"
     CDS             complement(28712..29521)
                     /gene="mhpD"
                     /locus_tag="R8G18_001710"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEE9795431.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(29531..30397)
                     /gene="mhpC"
                     /locus_tag="R8G18_001711"
     CDS             complement(29531..30397)
                     /gene="mhpC"
                     /locus_tag="R8G18_001711"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HEE9795432.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(30415..31359)
                     /gene="mhpB"
                     /locus_tag="R8G18_001712"
     CDS             complement(30415..31359)
                     /gene="mhpB"
                     /locus_tag="R8G18_001712"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HEE9795433.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(31361..33025)
                     /locus_tag="R8G18_001713"
     CDS             complement(31361..33025)
                     /locus_tag="R8G18_001713"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HEE9795434.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            33067..33900
                     /gene="mhpR"
                     /locus_tag="R8G18_001714"
                     /pseudo
     CDS             33067..33900
                     /gene="mhpR"
                     /locus_tag="R8G18_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            33977..35059
                     /gene="lacI"
                     /locus_tag="R8G18_001715"
     CDS             33977..35059
                     /gene="lacI"
                     /locus_tag="R8G18_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HEE9795435.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            35182..38256
                     /gene="lacZ"
                     /locus_tag="R8G18_001716"
     CDS             35182..38256
                     /gene="lacZ"
                     /locus_tag="R8G18_001716"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEE9795436.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            38308..>38572
                     /locus_tag="R8G18_001717"
     CDS             38308..>38572
                     /locus_tag="R8G18_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9795437.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         9378 a        10291 c         9634 g         9269 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt attattttta
     3241 tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa acgtttctct
     3301 tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt attataaata
     3361 aaagttatga aatatattct cttcgttaac ttataattaa gagaaaaaaa ctcaccgcat
     3421 tgccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat ctcgcgcaca
     3481 tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc agaagattgc
     3541 aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg
     3601 ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt ttggtggtaa
     3661 atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa
     3721 aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc acgtcagcga
     3781 tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc gcccttcggc
     3841 gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg cgcaaaacgg
     3901 tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc tgggcgaaga
     3961 tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct
     4021 ggcttcagca gcctgtatgg ataaagatgt caccaaacgt ctgctacgcg atgccgggct
     4081 gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca gttttgccga
     4141 agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg gctcttctgt
     4201 tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc tggcgttcga
     4261 gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg aatgcgcagt
     4321 tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca ccagcgattt
     4381 ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag ttccggcagc
     4441 cattgcacca gagatcaacg ataagatccg ggcgattgcc gttcaggctt atcagacgtt
     4501 gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg aagtggtgat
     4561 caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga agctgtggca
     4621 agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg cgctggagcg
     4681 tcacgctgcg gataacgcat tgaaaaccac aatgtaatat tttaacgccc gataatattc
     4741 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg ccagccagaa
     4801 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     4861 cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     4921 ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga aaaacaacat
     4981 ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa ttgttaataa
     5041 atttaactta aggttgccat tttgtaactc acaacgaaga ttcacgcttc gggaatattc
     5101 ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc agacttgcca
     5161 taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga
     5221 tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt caacgcctgg
     5281 caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa
     5341 cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg aaacaggtcg
     5401 cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc cgtttttagt
     5461 aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc gccgccagta
     5521 agatttacgt tgccagatac ggcctaaaac gccttatctg gcatgacatt cctgtttgat
     5581 cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc
     5641 aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt
     5701 gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc cgtctcctca
     5761 aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct catccccagt
     5821 tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact gtcgtaacga
     5881 attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgactgc gttctgaaac
     5941 gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta tatcggtgcg
     6001 ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcggcga
     6061 ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt ggtatgctgt
     6121 tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt aagcgaacca
     6181 aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc gctcaattgc
     6241 ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg cgatttgatt
     6301 tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc
     6361 tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata atttgactcc
     6421 tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt
     6481 tcaaaggtga tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatc
     6541 ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa cggttgcgga
     6601 gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga agatagatga
     6661 ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg
     6721 tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc
     6781 agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgttg
     6841 gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg aaacagcaag
     6901 aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt gaaatacata
     6961 tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta
     7021 ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcggcc
     7081 tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagccat
     7141 ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg
     7201 ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag gaaggcgatc
     7261 aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga agcaaaacgc
     7321 atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg ttgccagttc
     7381 gccatgtaat attcgttcat cgccgtacgc cagcggaaca catagtgact gacaaagaag
     7441 ttgttcagta cactgatcac caccgcgatc agcgcaatcc ccagaaagac gcccacttcg
     7501 cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg aatcaaatca
     7561 tagaacggtg cataccaggc gttgacggcg accccgactt ccaccaaaaa ccaggtgacg
     7621 aagatgatca gtgcagtacc gagtattgac cagtattgcc aacggtgcgg gctgtagata
     7681 aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa
     7741 tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc
     7801 gccacccagt ctcccccacc ggcttgccag aagataacgg caatcaatgc ccaaacaaag
     7861 gccgagagaa aaaacgctcc cggctttggg aaaaaagact taaacatatg cactcctgct
     7921 aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccactac aaagcgtcag
     7981 ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata
     8041 agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga ttaacacgcg
     8101 caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg
     8161 tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat
     8221 acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt
     8281 ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg atcactgaac
     8341 cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg
     8401 caacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgt agttatggcg
     8461 aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt attgcttccc
     8521 tcaccaaact aatgaccagt gaaatgctgg tgaaattgct cgaccagggg accgtaaagt
     8581 taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccgacc tacaatggga
     8641 cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg
     8701 gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc tggaaatacc
     8761 tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct aaccttgcgt
     8821 tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg
     8881 aagagcagat tacccgtcca ttggggatga aagacaccac ctacaccccc tcaccggatc
     8941 agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg
     9001 caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg atgcagcagt
     9061 atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag acgttgattt
     9121 atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc
     9181 ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga
     9241 caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg
     9301 cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca
     9361 atgatctggt gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa
     9421 tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg
     9481 cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg tgtacgttga
     9541 tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc aatcattgat
     9601 aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgcac gtcgcgtaat
     9661 tccagaattc tattttgcca ggctaaccag taaaaagcgt ctcgccagag ttgattttgt
     9721 tcaataagcg taatggtttg tttggctggt agatgatatc ccgtacaatt tccttctgat
     9781 attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat aatataaggg
     9841 gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac tccctctaaa
     9901 ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt ttcattatta
     9961 aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga
    10021 ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg
    10081 ggcattattt agattaaatt accagtattt cttcggagtg aagaatatta ccaggtatat
    10141 ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag gtagttagca
    10201 tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc aacacgtacc
    10261 gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc gttgttagag
    10321 tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc gctgtaggtg
    10381 aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga ctgaccgtca
    10441 actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc agaaacgctg
    10501 ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggc tttcaagccg
    10561 aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct catggttgca
    10621 gacaggtcgt tgttgaagtg agagtagctc aagctaccat caacaaagac gttgttcgcg
    10681 aagtgagcag aagagtagat gtaggcagtc tggctgtctt gatctacctg accagaacgg
    10741 tcattcatgt cacctttagc gaagcctgca gccgcaccga cgatccactt agcgttgtta
    10801 ccgtcaactt tggtatcaac accgaccatg atgccgttaa catcctgatc atagttgatg
    10861 gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc gccgttatca
    10921 gccaggccat gacgagagtt ggtcagacga gtaccaacgg tgtcttgttc caggttccag
    10981 atattggtgt tcgcagacgg gatgctcagc gccatgttag cgtagtcggt cagctccatc
    11041 tgttgcagaa caacggtgtt accgcgctgt tcagcctgat aggtgtatgc acccaggtca
    11101 gctttgttag cagcagagaa ggtcgcgttg gttttggtat catcgatata gatgagctgc
    11161 ttacctgcgt aatcagcaat cgcaccagcg ccagtggagt tgtcgatacg aactttgtag
    11221 ttaccggttg cagctacaac gttgtctgca gagctgttgt caacttcgtc gccattgtta
    11281 atgtcgttgt taccattaac ggtcaagtga ccatcagagt tcatcgcaat aacaccataa
    11341 ccgtagttag ctttgctggt gtcgttggta cgatcgttga cgaggtctgc attcaggatg
    11401 taatcattac tatgaatgtc aaacacacct gcgtaaacat taccgttgtt atctttaacg
    11461 ttgccactgg tcagttccag agtatcagtt gcaaatacgc tggagatgtc attcatgttg
    11521 gtgtttacat ccagcacacc accgttagta acggtaatgg tattggcata cagcggtgca
    11581 tatgcccaag ctgaagcagt atcttcaccg aagttaacgg ttgagaaggt atcaatggtc
    11641 aggtgatcgg tgttaacttc accgttaact tccattgaag aaccaccgtt cagagtgatg
    11701 gtgtcagaag tcagagaaga atctgcactc acgttaacct gagaaccgct gttgatcgcc
    11761 agggtatcga tcagggaagt tttcgtggtt tcccaatcag aaccgttgtt cagaacaaca
    11821 tcgaatacgc cgctctggaa tgcgttagcg ttatcaacac gattccagcc atcttcagag
    11881 taagaagcgt acgggttcag gctgtttggt gcaacgtcgt agaaggcttc gtcagcaaca
    11941 acaacgtcat aatcaatatt cgctgaacca acccatttgg tgttgttcag agtcaggttc
    12001 aggtgatcga cgttctgatc gtcagttgcc caaccgccat gagtgtaagc agtggtagag
    12061 ttgtcaatca ggtgacccgt cgcgtcaaag ttagcgttcc aggtactgtc aaatacaacg
    12121 tcaccaagca actgagagtt gttgaacgta acgttgttga ccatacgata atcagaagtg
    12181 tcatcatcga tgaaggctaa cgcaacatca tcagcaggag atgcgccatt gccgtagtca
    12241 cttggttcaa cagaattgcc gaaatgacca cgatctgatt gttcgtcttc agtccatgaa
    12301 ccagaagtta cagtagagtc attaacaacg atgttgttgt cccactgatg accttcggta
    12361 ttgtaggtcg cattaccgag gtcgtgttga accaaagtaa tacctgcaac atgggagttg
    12421 ttgctgatgt tgatgttact ttcaacatcc agagtcacag cggtaccaag accagcggtt
    12481 accaaacgat cataatctgt cttagatgtt ttaccgtctg catccagata agtatcggtg
    12541 aagtagaagg cgtcaaaatc atcgtcaata gtagaattag cgatgttcag agtgaagatg
    12601 tcgccatcaa cccagtcgtg gccagtataa tcgctaccgt tggtccagac gtagtcgcca
    12661 taaccaacct ggtaagatgt aaccatacca tgaacaattg agttggagat gttcagtgta
    12721 ttaccggtat ctgtgccata atcgtggttc agataataag tagaaattac accatttaca
    12781 gtgtcgccgc tgaaagtggt atcaaagtaa ccatcagctg cgtggtcata tacataacca
    12841 ccgtaagtca gataatcgtg atcaatttta gtgctgtcac cccaattgta gatggtggct
    12901 gcgtcatcgt gatacccgaa ggtgttataa gtagctgcat tagcctgaga ggtgatagcc
    12961 agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga gtgcatttgt
    13021 catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata gaaaacatat
    13081 tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta ttctttcccc
    13141 taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa ttgataaaag
    13201 gacgatattt caacatgttg cagataatgt tatttttgat acatacgcgt tataaatatc
    13261 aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat tattcaattt
    13321 atagcagcac aaccaaacgc caaaaaaata ataaccaaga gctataaata aagattaaaa
    13381 atattttcag tgaatatgaa attattgcta tttctattac aaagatgtcc tggtctctct
    13441 ttcattctct tcttgtcaac atcgcgacaa ctttcgtaaa acactcctgc cccgcttcag
    13501 gtatactgtg caccttaatt ccacaaacat caggcagacc atgacagact taatccaacg
    13561 ccctcgtcgc ctacgcaaat ctcctgcgct gcgcgctatg tttgaagaga caacacttag
    13621 ccttaacgac ctggtgttgc cgatctttgt tgaagaagaa attgacgact acaaagccgt
    13681 tgaagccatg ccaggcgtga tgcgcattcc agagaaacat ctggcacgcg aaattgaacg
    13741 catcgccaac gccggtatcc gttccgtaat gacttttggc atctctcacc ataccgatga
    13801 aaccggcagc gacgcctggc gggaagatgg actggtggcg cgtatgtcgc gcatctgcaa
    13861 gcagactgtg ccagaaatga tcgtcatgtc agacacttgt ttctgtgaat acacttctca
    13921 cggtcactgc ggtgtgctgt gcgagcatgg cgtcgacaac gacgcgactc tggaaaattt
    13981 aggcaagcaa gccgtggttg cagctgctgc aggcgcagac ttcatcgccc cttccgccgc
    14041 gatggacggc caggtacagg cgattcgtca ggcgctggac gctgcaggct ttaaagatac
    14101 ggcgattatg tcgtattcga ccaagttcgc ctcttccttt tatggcccgt tccgtgaagc
    14161 tgccggaagc gcattaaaag gcgaccgcaa aagctatcag atgaacccaa tgaaccgtcg
    14221 cgaggcgatt cgtgaatcac tgctggatga agcccagggc gcagactgcc tgatggttaa
    14281 acctgctgga gcgtacctcg acatcgtgcg tgagctgcgt gaacgtactg aattgccgat
    14341 tggcgcgtat caggtgagcg gtgagtacgc gatgattaag ttcgccgcgc tggcgggtgc
    14401 tatagatgaa gagaaagtcg tgctcgaaag cttaggttca attaagcgtg cgggtgcgga
    14461 tctgattttc agctactttg cgctggattt ggctgagaag aagattctgc gttaattttt
    14521 ttcctctcag aaagggggag ttaacctgtt gaaagtacgg tgaacgcccc ctcaccctaa
    14581 ccctctcccc aagggggcga gggaaccgtc cgcactcgta tcaccccgcc cgataaaacg
    14641 gtttatcccc aaggatcgtc gcccgatgca taatccgccg ctgtggcagg taatcggcat
    14701 tggcatagtg ctgagtcacg cggttatccc aaatcgcaat atcattcggt tgccagcgcc
    14761 aacgcacctg aaactccggt ttagtgatat gggcgaataa aaagcctaac aaggcttcgc
    14821 tctctttctc gctgacatcg acaattcgcg tagtaaagcc ttcattcaca aacagcgcct
    14881 gtttaccgct caccggatgc gttcgcacca ccggatgtag caacggagga tttttcgcga
    14941 ccgcctcgcg ccagcgttga tgttcctcct cggttttgcg gtatttgtat tccgggaacg
    15001 atttacggaa atcatgctcc gcacgcagcc cgctcagcag ctggcggaag ggaacagaga
    15061 gcgcctcata ggccgcaata ccgctggtcc agagcgtatc accgccggtc gaaggtaact
    15121 ctttagctgc cagaatcgcc cctgcgggtg gcgtttcaat aaatgtcaca tcggtatgcc
    15181 agttgtcgtt atccggcgga ttatcgttat gggtatccag cacgatgatc tcgtcaaccc
    15241 cttcggcatg agggtaaaca gggtgaatat gcagttcgcc aaaacgctgg gccagcgcgc
    15301 gttgctgctg cggcgtaata gcttgatcgc gcagaaatac cacctgatag cgcagcaccg
    15361 catggtaaag ctgttcaaac tgattatcgc ttaacgggcg cgtcaggtcg gcacccgaaa
    15421 tttgtgcgcc gatatacggc cccagcgggg taatactcag acgttcactc attgtacttc
    15481 tccatgccag ggcgtcagac ggcgctgtaa cgcgcgcaga cccagttcta aaagaaaggc
    15541 gataatcgca atcaccgcga tccccgccag taccacgtca gtcgcgagaa attcacccgc
    15601 tgactgaacc ataaatccta aaccgcgtgt cgcggcaatc agctccgccg ctaccagcgt
    15661 agaccagccc acacccagac caatgcgtaa tccggtgagg atttccggca gcgcgccggg
    15721 caaaatgaca aaccacagca cctgcgcacg gctggcaccc agcgactggg cggcacgaat
    15781 gcgaacctgc tgcgcgcttt tcacccccgc cagcaccgac atcgccaccg gtgcaaaaat
    15841 cgctaaatag atcaataaga tcttcgaggt ttcaccaata ccaaaccaga tcaccatcag
    15901 cggcaaataa gccagcggcg gcaccggacg ataaagttcg attaccggat ccagaatgcc
    15961 gcgcaccgtc gggcttagcc ccatggcgat cccgaccgga ataccgatca ccaccgccgc
    16021 gagtagcgcc agcacaatgc gcgtcaggct ggctgccaga tgctgccaca gcgtggcgtc
    16081 cataaatccc tgcggtccgg caatggtgag cagttttgcc agtacttgtt gcggcggcgg
    16141 caaaaacagc gggctaatca gttgcagcgc cgccaccgtc caccatacag tgagtaaaac
    16201 cgctaacgtg ccaatgctta aggtcacctg acgcgagagc ggccagcgcc atttcagccg
    16261 ccgcgaatgc agtttttcat taatgagcac actcatgaga acgcctcccg ttgctcaaat
    16321 acgcggctta aaacatattc gcgcatggcg atgaattgtg gatcggactt gatgctgcgg
    16381 ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg gcagccgctc cagcacacgg
    16441 ccagggccgg atgaaagcag aaccagttca gtcgccataa acaccgcttc ttctatatcg
    16501 tgggtaatca acagcacctg cttgcccgtc tcctgccaga gtttcagcag cagagtttgc
    16561 atctggtcgc gggtgaaggc gtccagcgca ccaaacggtt cgtcgagtaa taacagctgg
    16621 ggattcgccg ccagcgcgcg ggcaatcccc acccgctgac gttgaccacc ggaaagctgc
    16681 cagatatagc gtttttctgc gccttccagc cccacttttt tcagcatctg gtgcgcgatt
    16741 tccagtcgct gcattttctc tatacctgcc aattgcaggc cgaacgccac gttgtcctgt
    16801 acattgcgcc acggtagtag cccttcattc tgaaaaacca ccccgcgctc tgctcccggt
    16861 ccctcaatac gcttacccgc cagttgaatg ctgccatgct gataaggcac aaaaccggca
    16921 atcagattca gcagggtggt tttaccgcag ccggacggcc ccagcaccac cagtagctcg
    16981 ccgctttcca gcgtcaggtt gatatcttcc agtgccggtt tgccgccata atcggcatag
    17041 agatgagaga tttgcagcat ctgcgcctcc ttttattgca cgaagcgcga ggtaacgtac
    17101 tggctgtaat cattcgctac agccgggacc ttgccctgct ctttcaaaaa ctgcgcggtg
    17161 tcgatgattg ctttgttcac cggtccggtc agttctgccg tttgttgctg cggcgtcaga
    17221 taggtattcc ccttcaccag ccccggaacg tcaccttccg gcacgccgct taaacgcgcc
    17281 agtttgctga tgttttccgg ctgtttcagc cacgcgtctg ggttagcaat gtacggttgc
    17341 tgagcatcga tggcgctttt agcgaacgct ttcacgacct caggatgttt ctcggcaaaa
    17401 tctttgcgca ccacccagac gtccagcgtt ggcgcacccc actgcccgac ctgttcagaa
    17461 tcggtcaaca ccttgccgtc tttttccagg gcgttaaccg ccggtgccca gacataagca
    17521 ccatcaatat caccccgctg ccatgcggcg ataatcgcgg gcggctgcag gttcacaatc
    17581 tccacttgcc ctggtttaat cccccagtgt ttcagcgccg ccagcaggct gtagtgggtg
    17641 gtggagataa acggtacggc aatgcgtttg ccaatcaaat cttcgggttt gctgatagtt
    17701 ttctttacca ccagcgcttc ggagttaccc agttttgacg ccagcaagaa gacttcaatc
    17761 ggcacctgtt ggctggctgc aaccgctaat gggctggaac cgaggttgcc gatttgcacg
    17821 tcgccagaag ccagcgcccg cacgatgctg gctccgctgt caaacttacg ccagtccacg
    17881 ctcgccccgc tctctttagc aaaggtgttg tcggcctgag ccactttcgc cggttcggct
    17941 gaggtttgat acgccacggt gacgttcacc gcctgtgcct gaaaagcgat gaatgccagt
    18001 gcggcaagaa gtgtgttacg cgatgaaatt gccatgattg tctgctcccc tgtcttgtta
    18061 tgggagcagt attcaggaat aaaaacattc attaaaagaa ttagtcgtta tcgcacagat
    18121 gattttattc ttagcaaaaa aacggcaaag gaaagattaa atatgcaatt actccgttct
    18181 aacaattggg aaaattacta cacttcattt tagtgatgca taaacaacca cattacgaga
    18241 tttattactt cattgtttct atcaatacat ataataaaac tattatattc gcattgtata
    18301 tatcactgta tcattctcaa tcactcgcat attacattca cataatgtat ataaacaata
    18361 cttgttcaca gaacatattt tttaaatgca attaattgac aaactaaatc acatctaaat
    18421 acaatgtata aaacaaaata actatacata ccaaacatac aaacacttaa catatgaatt
    18481 gcataaaaac attacccagt cgaacctccg ggtctgaaag cccttgcttt tcagttatta
    18541 gcaacattta acatttataa attaacatct ggctgcgcca gagattacgt ctctgagtgc
    18601 atcgttgtgc ttgtagtata ataaataaaa tggagtgtca ttaagtggat aaggttttag
    18661 attcagccct cctttcttcg gcaaataaaa gaaagggtat ccttgctatt ggcgcacatc
    18721 ctgacgatat agaattaggc tgtggcgcat cgcttgctcg tcttgcgcaa aaaggaattt
    18781 atatcgcagc agtggtgatg actaccggca actctggtac agatggaata atcgatcgcc
    18841 atgaagaatc gcgcaacgcc ctaaagatat tagggtgcca ccaaactatt catcttaatt
    18901 ttgctgacac ccgcgctcat ttacagctca atgatatgat ttccgccctg gaagacatca
    18961 ttaaaaatca aattccttct gatgttgaaa tcatgcgggt atataccatg catgatgccg
    19021 accgccatca ggatcatctc gctgtttatc aagcttcaat ggttgcctgc cgcactattc
    19081 cacaaattct cggctacgaa accccaagta cctggctttc atttatgcct caggtttttg
    19141 aatccgttaa agaagaatat ttcacagtca aacttgctgc attaaaaaaa cataaaagcc
    19201 aggaacgacg tgattatatg cgtcatgatc gcctgcgtgc agttgcacaa tttcgcgggc
    19261 aacaggtcaa tagcgatctg ggtgaaggct ttgtaattca taaaatgatt ctttgagcaa
    19321 attgcatgaa aacctggata tttatctgta tgtccatagc aatgttgcta tggtttttaa
    19381 gtacgctaag acgtaaaccc agtcaaaaga aaggctgtat tgacgccatt atacctgcgt
    19441 ataacgaagg cccgtgtctg gcgcagtcac tggataatct actgcgtaac ccttattttt
    19501 gccgggtaat ttgcgttaac gacggctcca cggacaatac cgaagcggtc atggcggaag
    19561 tcaaacgcaa atggggcgac cgctttgttg ccgtcacgca aaaaaatacc ggtaaaggtg
    19621 gtgcgctgat gaatggcctc aattatgcca cctgcgacca ggttttttta agtgatgccg
    19681 acacctatgt tccgcccgat caagacggaa tgggctatat gctggcagaa attaagcgcg
    19741 gtgctgatgc cgtaggcggc attccctcta ctgcgttgaa aggcgcgggg ctgttaccgc
    19801 acatccgcgc gaccgtaaag ttgccgatga ttgttatgaa gcgcacgcta cagcagctcc
    19861 tgggcggcgc accgtttatt atcagcggtg cctgcggaat gttccgtact gatgtattgc
    19921 gtaagttcgg tttctcggat cgtactaaag tcgaagacct tgatctcacc tggacattag
    19981 tggcaaacgg ctaccgtatt cggcaggcga atcgctgcat cgtataccca caggaatgca
    20041 acagtccgcg tgaggagtgg cgtcgctggc ggcgttggat tgtgggctac gcggtctgta
    20101 tgcgcctgca taaaaggctt ttatttagcc gcttcggtat cttcagtata tttcctatgc
    20161 tgttggttgt gatttatggc gttgggattt atctcactac ctggtttaat gaattcatca
    20221 ccaccgggcc gcatggtgtg gtgttggcaa tgtttccgct tatctggatc ggcgtagttt
    20281 gtgttattgg tgcttttagc gcctggtttc atcgttgctg gttgttggtg cctttagcgc
    20341 cgctttccgt tgtgtatgta ttattagctt atgccatctg gattatctat ggacttattg
    20401 ccttttttac tggacgcgaa cctcagcgcg acaaacccac ccgctattcc gcactggtgg
    20461 aagcgtcaac cgcttattcc caaccttctg tcacaggaac tgaaaaacta tctgaagctt
    20521 aatgttaaag agaaaaatta ttcagattgc agaccaggta attattgatg aaactgcagg
    20581 tgaagttgtt atcggcgcga atacccgtat ttgtcatggt gccgttattc agggtccggt
    20641 agtgattggc gcaaactgcc tgataggtaa ttatgcgttt attcgtcctg gcacaataat
    20701 cagcaatggt gtaaaaattg gttttgccac cgaaattaaa aatgcggtta ttgaagcgga
    20761 agcaacgatt ggtccgcaat gttatattgc cgactcggta gttgcaaacc aggcatattt
    20821 gggcgcacaa gtacgtacca gtaatcatcg tctggatgaa cagcctgtgt ctgttcgaac
    20881 tccagaggga attatcgcta ccggatgcga taaattaggt tgttatatcg ggcagcgttc
    20941 acgccttggc gtacaagtta ttattttgcc tgggcgaatt atttccccga acacgcaact
    21001 tggcccgcgc gtgattgtag aacgtaattt acctaccgga acttattcac tccgacaaga
    21061 acttatccgt acaggagatt aaaatgataa aacggacgtt attagcggcg gccattttta
    21121 gcgcattgcc cgcttatgcc gggttaactt ccattaccgc gggctacgat tttaccgatt
    21181 attctggcga tcatggcaac cgtaatttag cgtatgctga actggtggcg aaagttgaaa
    21241 acgcaacgct gctttttaat ctttcccagg ggcgtcgtga ttatgaaact gaacatttca
    21301 atgccactcg cggtcagggt gcggtctggt ataaatggaa taactggctg acaacccgaa
    21361 cgggtattgc ctttgcggat aatacgccgg tctttgcccg ccaggatttt cgtcaggata
    21421 ttaacctggc cctgttgcca aaaacgcttt tcacgaccgg ttatcgctac actaaatatt
    21481 acgatgatgt cgaagtcgat gcctggcaag gcggcgtatc actctatact ggcccggtga
    21541 tcaccagcta ccgctatacc cattatgact ccagcgatgc aggtggtagt tatagcaata
    21601 tgatttccgt gcgtctgaat gacccgcgcg gcactggtta tacgcaacta tggctaagcc
    21661 gcggaacagg cgcttacacc tatgactgga cgccagaaac acgctacggc agcatgaaga
    21721 gcgtcagttt gcaacgtatt caaccgctga ctgagcaact taatctcggg ctgacggcag
    21781 gtaaagtgtg gtacgacacc ccaaccgatg attataacgg tctgcaactt gcagcccatc
    21841 tgacctggaa attctgattg cttctgccgc ccgctatccg gggcggcctt ccctgccgat
    21901 tagccccact ccctttcctc gttgttttcc gaccacattc accggataaa ttttattctc
    21961 cagcgttata tactataggg aggtatgcat tgacatatag catacctccc tatagtatat
    22021 tgcgtgcaga tgatgaggtg cgaaatgccc agtactccgg aagagaagaa aaaggtcctt
    22081 actcgagttc gtcgtattcg ggggcagatt gatgctctgg aacggtcgct ggagggtgat
    22141 gccgaatgcc gtgccatact ccaacagatc gctgccgttc ggggcgcggc taacgggctg
    22201 atggcagaag tgcttgaaag ccatatccgg gaaacgtttg accgaaatga ctgctacagc
    22261 cgcgaagtca gccaatccgt tgacgacact attgaactgg ttcgagccta tcttaaatag
    22321 ctgaatctat taccatattg aggaagagcg agagatgaaa tcacgtgctg ccgttgcatt
    22381 tgctcccggt aaaccgctgg aaatcgttga aattgacgtt gcaccaccga aaaaaggtga
    22441 agtgctgatt aaagtcaccc ataccggcgt ttgccatacc gacgcattta ccctctccgg
    22501 ggatgacccg gaaggtgtat tcccggtggt tctcggtcac gaaggggccg gcgttgtggt
    22561 tgaagtcggt gaaggcgtaa ccagcgtcaa acctggcgac catgtgatcc cgctttacac
    22621 cgcggagtgc ggcgagtgtg agttctgtcg ttctggcaaa actaacctct gtgttgcggt
    22681 tcgcgaaacc cagggtaaag gcttgatgcc agacggcacc acccgttttt cttacaacgg
    22741 gcagccgctt tatcactaca tggggtgctc tacattcagt gaatacaccg tggtcgcgga
    22801 agtgtctctg gccaaaatta atccagaagc aaaccatgaa cacgtctgcc tgctgggctg
    22861 tggcgtgacc accggtattg gcgcggtgca caacacagct aaagtccagc caggtgattc
    22921 tgttgccgtg tttggtcttg gcgcgattgg tctggcagtg gttcagggcg cgcgtcaggc
    22981 gaaagcggga cggattatcg ctatcgatac caacccgaag aaattcgatc tggcgcgtcg
    23041 cttcggtgct accgactgca ttaacccgaa tgactacgac aaaccgatta aagatgtcct
    23101 gctggatatc aacaaatggg gtatcgacca tacctttgaa tgcatcggta acgtcaacgt
    23161 gatgcgtgcg gcgctggaaa gtgcgcaccg cggctggggt cagtcggtga tcatcggggt
    23221 ggcaggttct ggtcaggaaa tctccacccg tccattccag ttggtcactg gtcgcgtatg
    23281 gaaaggttcc gcgtttggcg gcgtgaaagg tcgttcccag ttaccgggta tggttgaaga
    23341 tgcgatgaaa ggtgatatcg atctggaacc gtttgtcacg cataccatga gccttgatga
    23401 aattaatgac gccttcgacc tgatgcatga aggcaaatcc attcgaaccg taattcgtta
    23461 ctgatttccc gcaggtatac cccgtccact tcagacgggg ttcttaatac tctccccggg
    23521 cagccgtccg ggggattaac cgtgagataa tgactgatgg aactcattga aaaacatgcc
    23581 agctttggcg gctggcaaaa tgtgtatcgg cattattccc aatcactgaa atgtgaaatg
    23641 aatgtcggcg tctatctccc accaaaagcc gcgaatgaaa aattgccggt gctgtactgg
    23701 ctttcaggcc tgacctgcaa cgagcagaat ttcattacta aatcggggat gcagcgttac
    23761 gcggctgagc acaacattat tgttgttgcg ccggacacca gtccgcgagg cagtcatgtc
    23821 gcagatgccg accgttacga tctcgggcaa ggtgccgggt tttacctgaa cgcaacgcaa
    23881 gcgccgtgga atgaacatta caaaatgtat gactatatcc gcaacgagct gccggattta
    23941 gtgatgcaac attttccggc aacgaccaga aagtctatct ctggtcattc tatgggcggg
    24001 ctgggcgcgc tggtgctggc gttacgtaac ccagatgaat atgtcagcgt ctcggcgttt
    24061 tcgcccattg tctccccatc gcaagtaccg tggggacagc aagcctttgc cgcatatctt
    24121 ggtgaaaata aagatgcctg gttggattac gatccggtga gtcttatttc acaaggtcaa
    24181 cgcgttgcgg aaatcatggt tgatcagggg ttgagtgatg atttttacgc ggaacagctg
    24241 cggactccaa atcttgaaaa gatctgccag gagatgaata tcaagacgtt aatccgttat
    24301 cacgagggtt acgaccacag ctattatttt gtctccagtt ttattggcga gcatattgcc
    24361 taccacgcca ataaactgaa tatgcgttga taatagtgca cgactgccgg atgcggcgtg
    24421 aacgccttat ccggcctaca cttcgcccgt aaaccgtagg cctgataaga cgcgcaaagc
    24481 gtcgcatcag gcatggcgca cgactgccgg atgcggcgtg aacgccttat ccggtctaca
    24541 cttcgcccat aaaccgtagg cctgataaga cgcgcatagc gtcgcatcag gcatctgcgc
    24601 acgacttatc tatgtttacc acatcaaatc atcaggcact ttgaagtcgg catacggatc
    24661 gtcttcatcc tgctcttcgg cactgagcgc gctgtgcaac acaatactgc tggcatcacg
    24721 ctgagcaatt ttatcggcaa cactggcggg aataatggtg tattcacctt cgctattgtt
    24781 atcgaccaac aagcgggcaa tcgccagacg accattaatc aactgcgcct gtgtgagctt
    24841 atcgacaaaa atcttcttaa tcagattgcc gtcagtgaag ttaaaaccaa tatcgccatt
    24901 ggcaatggtg attcggttca tttcaatgag ctgcttcacc tgggctttat attctttcgc
    24961 caacgccgct tgtttttgct gttcgctaag ctgtttatca cgctcaagct gtgccttttt
    25021 attttcttct accgcagccc gagcttcacg cgcctgaacg cgcgattttt tcgccgttct
    25081 ctcgaccttc gccgcttttt tgctggtgac taatcctgct ttgagcaact gctcttgtaa
    25141 ggtaagtttt gccatttttg tttccaaacc cgctgaataa tttttagcga gtatacctgt
    25201 aaacgccgtg ggtgtattgg gggcaagtcc ctttcgcccc tgcttaataa ggcgcgtcag
    25261 gcatcggcac acggctgcat tcttgatctc cgactcatca aaataaacac cgccaaccca
    25321 gcaacaagaa tacccggtgc agaagcggcc attacgccga ccgtgccagt gcctaatgcc
    25381 agcattttcc cggccagtaa cggaccgctc atagccccca gacgccctac cgccacggct
    25441 gttcccacac ctgttgcgcg gatctgcgaa ctgtaaaaca acggtgccag ggcatacaaa
    25501 acgctttgcc cacctgtcgc aaacaacccc gcgacaaatc ccgccagcaa cataccgtta
    25561 aatgacgaca ccgttccaag cgccagcagc gaagctaaca tgccgctata aatcagtagc
    25621 gacatggtta ctggacgcag cttatccatc aatgcgccca acattaacgt cccgcttgcc
    25681 gcccccattt gcagggcaaa catcacccct gccgcctgcg atagctggaa tccttgctcc
    25741 accaaaagta gcggtagcca gttgatcaac atataaacca ccagcagagt gaagaaataa
    25801 cacaaccaca gcagcagcgt cgcggttgcc gtttctggcg caaataaggc acgcagtggt
    25861 ggcgcagcct gtttttcgcc agcgaaaacc gctgactccg gcagccagcg cattaatagc
    25921 ggcaccagaa tcaacggcac cacaccacct acccaaaaca ccgtttgcca tgctaagttt
    25981 gcccccgcga aacccagtgt cgccgccagc gccgcgccaa tgggaacacc gcaatacatc
    26041 aggctcactg ccgtcccacg aaaacgtgga cccgcggctt cagatgtcag ggcgataaga
    26101 ttcggcaacg ccgcccccag cccgacaccg gtcatcagcc gcgcaaagac cagtgagggg
    26161 aaatcccagg caatcgccgt tgccagtgag aacaaaccaa acagcgcaac tgagccaatc
    26221 aaaatacgct tacgaccata acggtctgcc agcatcccgc caaccaacgc gccgggtagc
    26281 aaaccgagta ttccggcgct aaatatccag cccatttgca ttttatcgag tgcgaaagcc
    26341 tgggcgatgc cacccgccgc aatgccagcc gcctgaagat ccagcccttc catcagagcg
    26401 accaaaaaac aaagcccgat ggtcagcatc aggcgggatg aagatgatga aggggtacga
    26461 gtcgacataa gatgaacctc attgcgagtg caggtgaatc gcgactgata cattgccagc
    26521 cgcgtaatca gaactattta atgactgcct acgggcgaaa tgtagcgaaa tgtaggccgg
    26581 ataaggcgtt cacgccgcat ccggcagtcg tgcgcagatg cctgatgcga cgctaacgcg
    26641 tcttatcaga cctacaaatt ctcacgccat ccgtaggccg gacaaggcgt tcacgccgca
    26701 tccggctgtc gtgctgggat gggcatacaa aatgcacgtt atcggcagtc atcattattt
    26761 gttgttgcgc agatccagcg ccacgtcgac gatcatatcc tcctggccgc caaccatccg
    26821 gcgtttgccc agctcaacga gaatatccac cgcacttaag ccataacgcg ccgccgccgt
    26881 ttcacagtga cgcaggaagc tcgagtaaac accagcgtat cccagcgcca gcgtttcgcg
    26941 atcgactcgt accggacgat cctgcaacgg acgcaccagg tcgtcggcgg catccattaa
    27001 cgcatagaga tcggtcccat gctgccagcc cagtttatcc gcggcggcaa taaacacttc
    27061 cagcggtgcg ttacctgcgc ccgcgcccat tcccgcgagg ctggcgtcga ttcggtcgca
    27121 gccctcttcc accgccgcga tagagttcgc cacgccaaga ctcaggttat ggtgagcgtg
    27181 catgccggtt tgcgtttctg gtttcaacac tgctttcagg gcgcggaaac ggtcacggat
    27241 atcgctcatg ttcatcgcac cgccagaatc caccacataa atacaggtcg caccgtagcc
    27301 ttccatcagc tttgcctgct tggcgagatt ctccggcgtg gtcatatggc tcatcatcag
    27361 aaaaccaacg gtgtccattc cgagctcgcg ggcatactga atatgctggg cggaaacatc
    27421 agcttcggta cagtgcgttg ccacacgaac cacccgcgcg ccagcctgcc aggcattttt
    27481 cagatcgtga atagtgccga ttcctggcag caacaacgtc gcgattttgg cgtgcctcac
    27541 cacatccgcc gccgcttcaa tccattcaag gtcgctatgt gcgccgaaac catagttaaa
    27601 gctggaacct tgcaaaccgt cgccgtgggc cacttcaatc gaatccacgc gggcatcgtc
    27661 cagtgctttg gcaatctggc gaacgttttc cagcgaatac tgatgacgaa tggcgtgcat
    27721 accgtcacgc aatgtgacgt ccgagatata aagtttttta ccgttcatgc cgcttctcct
    27781 gccttgcgcg ccagtgactg ggccattttt tccgctgtcg ccagcgcact ggaagtcata
    27841 atatcgaggt tgcccgcata ggcaggcaga taatgcgctg cgccttcgac ttccagccag
    27901 accgctgttt tcagtccgga gaattgcccc acgcccggta aattgaccgg tttatcctgc
    27961 gggataactt caaactgcac gcgctgtttc aggcgataac ccggtacgta agcctgcacc
    28021 gcctcagcca tttcattgat tgaggcttcg atatcatctt gtgaagcttc gtcgctcaat
    28081 acatacaccg tgtcacgcat catcaacggt ggctctgctg ggttaagcac aataatcgcc
    28141 ttcccttttg ccgcgccgcc caccacttca atggctcggg aagtggtttc cgtaaattca
    28201 tcgatattgg cacgcgtgcc aggtccggca gatttactgg cgatagaagc gataatttcg
    28261 gcgtaatgaa cacgcgccac gcgtgaaact gccgccacca ttggaatggt ggcctggccg
    28321 ccgcaggtga ccatgttgac gttcagttga tcgacgttcg cctcgaggtt aaccaccggc
    28381 acgcagtaag ggccgatggc agcaggcgtc aggtcaatta agcgaatatc cggtttcgct
    28441 tcgcgtaaag cggcatcgtt tttcacatgc gcgcccgcgc tggtcgcatc aaatacaatg
    28501 tcgatatcag caaattcagg catgttcatc aacccgatca ccccttcatg ggtggtggcg
    28561 acgcccatac gtctggcacg cgccagaccg tcggactgag gatcaatgcc gaccatcacc
    28621 gccatctcca gatgctgacc gtgacgcaaa attttaatca tcagatcggt gccaatgttg
    28681 ccagaaccga taatggcgac tttacgctta ctcatgacag acttcctttt ggggctgcgc
    28741 ttgaaaatgt cgcagcaact gaacctatgc cttcaatatg ggcttcaaaa cgatcgcccg
    28801 cattcaccgc caccatcggg cctaatgccc cggtaagaat gatatctccg gcgcgcagcg
    28861 gttcacccag actggccatt ttgcgtgcca gccagacggc ggcattaagc ggatgtccca
    28921 ggcattcgct gccgcgcccg ctggaaacct cttcgttatt acgcgtcatc ttcatggcgc
    28981 agtttttcag gtctaacccc gccggacgtt gcgccggacc gccgatgaca tacaccccgc
    29041 aggaagcgtt atctgccacg gtatcgacaa actgaatcga ccagtcgcga atgcggctcc
    29101 ccaccacttc cagcgccgga agtacccatt caatggcgtt atacagttcg tcgaaggtga
    29161 tatcagttgc gggtaaatcg cggttcaaca ccagtgcgat ctccgcttca atgcggggtt
    29221 gcagaacacg ggaaaaagga atgatttcgt tatcgccata acacatgtcg gcaaataacg
    29281 tcccaaaatc cggttgatca acgcccagtt gttgttgcac tttcggatgc gtcaggccca
    29341 ctttacgccc taccacgcga cgcccctgca caacgtcata ttgcacattt atgtgctgaa
    29401 tggcgtaggc cgcttcagcg tgatcgatac caatcagatc gcgcagcggc gcaatcgctt
    29461 cgccctgctc tgcggcgcgg cgtaaatccg ccgccagttg ctcaagagta tgcttcgtca
    29521 tgaccattcc ttaagggcgt gcgaggaaat tcagcaccaa ttgattgaaa gcgtcggcat
    29581 gttcccactg cgcccagtga ccacagtcgc ggaagatatg cagttcagaa ccggcaatgc
    29641 cggacagcag acgcagaccc gcatccatcg gcacaaagcg gtcgttgcgc ccccagacaa
    29701 tcagggtttg cgctttgatt tccgccagac gtgggccaaa atccgggaac tgtttcggat
    29761 tagcttccag gctcttaacg aagttttcca ggtgatcgcg gcgcgacagc atattattca
    29821 ggcgcgcttc aaacagggcg tcggtcaaat cgctggtatc gaaaacgaag atatccatca
    29881 tcagcttcag gttttcgata gtcggctgac gataaagctg attcagtcgc ttaatacctt
    29941 cggttggcat cggcgtaaac aaactcatgc cgcccgtacc gccgcccatc agcaccagtt
    30001 tgccgacgcg ctccggccag ttaagggtga acgccacaga actatggccg cccatcgagt
    30061 tgcccagcag gtggattttg gcgatatcca gttgatccac cacgcttttc aggattcgtg
    30121 cattaagatc cgatcgtgaa ccactattaa cgatcgaatc gcttttgccc caacccggac
    30181 aatccagcag gatcacccga tagcccgcct ctaccagcgg atcgatattg cggctgaagt
    30241 tcgcccagcc agtagcaccc gggccggaac catgcagcag gacaacggtt tcgtcgcctt
    30301 gtccgcagtc attaaaatgg atgcgcagcg ttttacccgc ttcttctaca ttcagaaaac
    30361 ggctggtggc ggcttcggtt tgtggctgat aactcatcat cttctcctgc atattcagtt
    30421 ctctgttctg gcgcttaacg agccaaatcc ggcaatccac tccgggattg ggcggtaata
    30481 acgcccttcg ctacgccagt tgccaaatgc agaaatagcg gcaaaagcgg cgacccaggt
    30541 tttgatttca tgtgtcgact ttccggcaat ggcggaaagc tcttcgttac tgacggcatc
    30601 cagttcctgt atgcgtccct gctccagcaa agtcatgaac tggttatccc aaatcgggtt
    30661 gagcggatgc agagttctct gatcctcaac aaacttctca gcggcgctaa tcaccctttg
    30721 ctgacgcaat tcgcgctcac tggcgggtaa atctttcccg ctccccaaca gacggtcgcg
    30781 catatgggca tcggctttcg ccagttcggg caccggcggc tgatgggaaa gcccaccgga
    30841 acccaggaac agcacgcgtt tattgagagt gctggtgaaa cgtccaatgg cttcacccaa
    30901 catgcgggta cgctggaaac cgggcagcgg cgtggcgaca ccgttgatga acacaggcag
    30961 aactggcacc ttatccagcc caccgagcag gaactccagc ggctgggcga acccgtggtc
    31021 cacctgcata cagtaagaaa cggcaagatc gatcccgctc ttcatgacag catgcgcaca
    31081 ggcctccgcc agctccacag gcacgggcag ctctcctgcc gcactgccga aatcaccaat
    31141 tgccgtcgct ccaacgccta aacagaacgg tggcatcacg tcatagaaaa agccgttgta
    31201 gtgatctggc gcaaacagca ccaccagttc aggggagaaa gccgcaatac gctcgcgggc
    31261 gctggcaatc acgccattga cctcatcgag tacctcttgc gccgggtcga cataccccac
    31321 caacggcgag tgggaaagac agtgaagata agcgtgcata tcaggctacc ttttcgacag
    31381 aaacgtcggc atcagggcgg gtcagcgtca tcaccgacgc cagtttattc agcgtattgc
    31441 ccagggtttg cggaatggcg gtggcggcaa caaagcgatc cgggcgcatc accaccagcg
    31501 aagcattgtg ttgcgcgaac cagctacgca gacgaccttg cgtatcgccc acgtgtagca
    31561 cgccgtcgtg gttatcctgt gcggtatgaa tttgcacttc cggcaccacc tgaatgaagc
    31621 gtgtgcccaa cgcgcgccac tgctggattt gctcgtcgct catcccccac agtggattgc
    31681 atccccagcc aattaccgcg aagttcgcgc cgatcgcgtt atcgagcagc gtcacgtcgc
    31741 cgttttccag cgtgactttc ggctgaataa acatcttgcc gaccggagag tgcttcgctt
    31801 cgccctcacg caccagcgca ccgccgtaat attgcggcat cggcttgaag cgcatttcga
    31861 ggaagtagcg ttttactggc ggcagataat tcaacagcca ggaaacgccg tcacgtaacg
    31921 taccctgcca gcgtttcggc ggagccagca cgttgcccgc cgtcacggac aggtcaatca
    31981 tcgctttggc gtgatcgcga cgttcttgtt gataggtatc gagcagcgca tcgcgggctt
    32041 tcccctggat aaccaacgcc agtttccagg cgaggttaaa ggcgtcgcgc ataccactgt
    32101 tatagccctg cccctgccac accggcatga tgtgcgcggc atcgcccgcc agcagtacgc
    32161 gatcaatacg gaaacgttgc gccagtcgcg cgttatgggt gtagacacgc tggcgaatca
    32221 attcaacatt gtccggatta ggcagcactt tgcttaacag cttgcgcata ttttgcggct
    32281 cacgcagctg ctcttcggtt tctcccggca tcaccataaa ttcaaagcga cgtaccgcat
    32341 gaggcagcgc ggcagaaaca tacgggcgca ccggatcgca acacaaatag atatgcggcg
    32401 tacttaacgg atcgttggcg atatctacca caatccactg atttggcgca gttttacctt
    32461 caaacggcac attcagggtg cgacgaacaa aacttgcccc accatcacag gccaccagcc
    32521 actgggcttt gatcgtttcc cgctgccctt ctgccgtttt caggtgcaag gtcacttcgt
    32581 catcttgctg gctgaaggcc tccagctcgc gggaaaacaa gcagcgcaca ttcggaaaac
    32641 gcgacaaccc ttccagcatc accgcatcga cctgtggctg aataaaggcg ttacggcgcg
    32701 gccagccaaa ttcatcggtc attggctgaa tatcagcaaa acagcggcct tttggggtga
    32761 gaaaacgcat cgcgtgccac ggtgtagtgt gcggcagaac attctcgacc aggccgaccg
    32821 actgcatggt gcgcagcgcc tcgtcatcaa taccaatcgc acgcgggtag tcgatcaact
    32881 tatcgagttt ctccaccacc agcacgtcaa tgcccatctg accgagatag ttcgccatca
    32941 tcagcccaac cggaccggca ccagcgatcg ccacctgaac gctatggtta acagcaggct
    33001 ggatgtcagg gtgttgtatt gccatttctg cacaacgttg ttttaactat agaaatgtca
    33061 attaatatgc agaacaatga gcagacggaa tacaaaaccg tgcgcggctt aacccgcggt
    33121 ctaatgttat taaatatgtt aaataaactt gatggcggtg ccagcgtcgg gctgctggcg
    33181 gaactcagcg gcctgcatcg caccactgtg cggcgactgc tggagacgct gcaggaagag
    33241 ggatatgtcc gccgtagccc ctccgatgac agttttcgtc tgaccatcaa agtgcggcaa
    33301 ttaagcgaag gatttcgtga cgaacagtgg atttctgcac tggcggcccc gctgctgggc
    33361 gatctgttgc gcgaagtggt atgaccaaca gatgtgtcca ccctggatgt tgatgcaatg
    33421 gtggtacgcg aaaccaccca ccgtttcagc cgcttatcct ttcaccgggc aatggtcggg
    33481 cgacgtttac cgctcctgaa aaccgcctcg ggcctgacct ggctggcctt ttgcccggaa
    33541 caagaacgca aggaattaat cgaaatgtta gccgcccgcc ccggtgatga ctatcaactg
    33601 gcacgagaac cgttaaagct gcaagccatt ctggcgcgcg cgcgcaaaga gggttacgga
    33661 cagaactacc gcggctggga tcaggaggag aagatcgcct ctatcgccgt accgctgcgc
    33721 agtgaacaac gggtgattgg ctgtctgaat ctggtgtata tggcgagcgc aatgaccatt
    33781 gaacaggcag cggaaaagca tcttccggcg ctacaacggg tagcaaaaca gatcgaagaa
    33841 ggggttgaat cgcaggctat tctggtggcc ggacggcgaa gcggcgtgca tttacgttga
    33901 caccatcgaa tggcgcaaaa cctttcgtgg tatggcatga tagcgcccgg aagagagtca
    33961 attcagggtg gtgaatgtga aaccagtaac gctatacgat gtcgcagagt atgccggtgt
    34021 ctcttatcag accgtttctc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
    34081 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
    34141 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
    34201 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
    34261 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
    34321 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
    34381 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
    34441 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
    34501 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
    34561 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
    34621 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
    34681 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
    34741 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
    34801 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
    34861 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
    34921 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
    34981 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    35041 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    35101 cccaggcttt acactttatg cttccggctc gtatgttgtg tgaaattgtg agcggataac
    35161 aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt cgttttacaa
    35221 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    35281 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    35341 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
    35401 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
    35461 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgcct
    35521 tttgttcccg cggagaatcc gacgggttgt tactcgctca catttaatat tgatgaaagc
    35581 tggctacagg aaggccagac gcgaattatt tttgatggtg ttaactcggc gtttcatctg
    35641 tggtgcaacg ggcgctgggt cggttacggc caggacagcc gtttgccgtc tgaatttgac
    35701 ctgagcgcat ttttacgcgc cggaaaaaac cgcctcgcgg tgatggtgct gcgctggagt
    35761 gacggtagtt atctggaaga tcaggatatg tggcgaatga gcggcatttt ccgtgacgtc
    35821 tcgttgctgc acaaaccgag cacacaaatc agcgatttcc atgttgccac tcactttaat
    35881 gatgatttca gccgcgcggt actggaggca gaagttcaga tgtacggcga gctgcgcgat
    35941 gagctgcggg tgacggtttc tttgtggcag ggtgaaacgc aggtcgccag cggcaccgcg
    36001 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacgctacgt
    36061 ctgaacgtcg aaaacccggc gctgtggagc gccgaaatcc cgaatctcta tcgtgcggtg
    36121 gttgaactgc acaccgacga cggcacgctg attgaagcag aagcctgcga tgtcggtttc
    36181 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcgc
    36241 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg
    36301 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgttg ttcgcattat
    36361 ccgaaccatc cgctgtggta cacgctgtgc gaccgcttcg gcttgtacgt ggtggatgaa
    36421 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg
    36481 ttacccgcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt
    36541 gtgatcatct ggtcgctggg gaatgagtca ggccacggcg ctaatcacga cgcactctat
    36601 cgctggatca aatctgtcga tccatcccgc ccggtgcagt atgaaggcgg cggagccgac
    36661 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc
    36721 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg
    36781 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggctt cgctaaatac
    36841 tggcaggcgt ttcgtcagta cccccgttta cagggcggct tcgtctggga ctgggtggat
    36901 cagtcgctga ttaaatatga tgaaaatggc aatccgtggt cggcttacgg cggtgatttt
    36961 ggcgatacac cgaacgatcg ccagttctgc atgaacggtc tggtctttgc cgaccgcacg
    37021 ccgcatccag cgctgacaga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc
    37081 gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagcttctg
    37141 cactggtcgg tggcgttgga tggcaagccg ctggcaagcg gtgaaatgcc tctggatgtt
    37201 gctccacaag ataaacagtt gattgaactg cctgaactac cgcagccgga gagcgccgga
    37261 caactctggc ttacggtaca cgtagtgcaa ccgaacgcga ccgcatggtc agaagccgga
    37321 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagcgtgac actccccgcc
    37381 gcgccccacg ccatcccgca actgaccacc agcgaaacgg atttttgcat cgagctgggt
    37441 aataaacgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgct
    37501 gaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac
    37561 attggcgtaa gtgaagcgac ccgcattgac ccgaacgcct gggtcgaacg ctggaaggcg
    37621 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgccgac
    37681 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg gaaaaacctt atttatcagc
    37741 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagta
    37801 gcgagcgata caccgcatcc ggctcggatt ggcctgacct gccagctggc gcaggtagca
    37861 gaacgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc
    37921 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg
    37981 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc
    38041 ggcgacttcc agttcaatat cagtcgctac agccaacaac aactgatgga aaccagccat
    38101 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg
    38161 attggtggcg acgactcctg gagcccgtca gtttcagcgg aattccagct tagcgccggt
    38221 agctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag gccatgtctg
    38281 cccgtatttc gcgtaaggaa atccattatg tactatttaa aaaacacaaa cttttggatg
    38341 ttcggtttat tctttttctt ttactttttt atcatggggg cctacttccc gtttttcccg
    38401 atttggctac atgacatcaa ccatatcagc aaaagtgata cgggtattat ttttgccgct
    38461 atttctctgt tctcgctatt attccaaccg ctgtttggtc tgctttctga caaactcggg
    38521 ctgcgcaaat acctgctgtg gattattacc ggcatgttag tgatgtttgc gc
//
LOCUS       DAQSWQ010000033        38337 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.033, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000033 DAQSWQ010000000
VERSION     DAQSWQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.033"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            59..1423
                     /locus_tag="R8G18_001718"
     CDS             59..1423
                     /locus_tag="R8G18_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9795438.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="R8G18_001719"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="R8G18_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HEE9795439.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="R8G18_001720"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="R8G18_001720"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEE9795440.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="R8G18_001721"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="R8G18_001721"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HEE9795441.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="R8G18_001722"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="R8G18_001722"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEE9795442.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="R8G18_001723"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="R8G18_001723"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEE9795443.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="R8G18_001724"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="R8G18_001724"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HEE9795444.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="R8G18_001725"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="R8G18_001725"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEE9795445.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="R8G18_001726"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="R8G18_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HEE9795446.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="R8G18_001727"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="R8G18_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEE9795447.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="R8G18_001728"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="R8G18_001728"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEE9795448.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="R8G18_001729"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="R8G18_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEE9795449.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="R8G18_001730"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="R8G18_001730"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEE9795450.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="R8G18_001731"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="R8G18_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEE9795451.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="R8G18_001732"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="R8G18_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEE9795452.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="R8G18_001733"
     CDS             13749..14288
                     /locus_tag="R8G18_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HEE9795453.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="R8G18_001734"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="R8G18_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HEE9795454.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /locus_tag="R8G18_001735"
     CDS             complement(15648..15995)
                     /locus_tag="R8G18_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HEE9795455.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="R8G18_001736"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="R8G18_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEE9795456.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="R8G18_001737"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="R8G18_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEE9795457.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="R8G18_001738"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="R8G18_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEE9795458.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="R8G18_001739"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="R8G18_001739"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEE9795459.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="R8G18_001740"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="R8G18_001740"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEE9795460.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="R8G18_001741"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="R8G18_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HEE9795461.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLTDFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="R8G18_001742"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="R8G18_001742"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HEE9795462.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="R8G18_001743"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="R8G18_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HEE9795463.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="R8G18_001744"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="R8G18_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HEE9795464.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="R8G18_001745"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="R8G18_001745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEE9795465.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="R8G18_001746"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="R8G18_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HEE9795466.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="R8G18_001747"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="R8G18_001747"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HEE9795467.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="R8G18_001748"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="R8G18_001748"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HEE9795468.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="R8G18_001749"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="R8G18_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HEE9795469.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="R8G18_001750"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="R8G18_001750"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HEE9795470.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="R8G18_001751"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="R8G18_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEE9795471.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="R8G18_001752"
     CDS             complement(37465..37920)
                     /locus_tag="R8G18_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795472.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="R8G18_001753"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="R8G18_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9466 a        10313 c         9734 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactgact gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cggcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactgacgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAQSWQ010000034        37258 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.034, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000034 DAQSWQ010000000
VERSION     DAQSWQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.034"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..643)
                     /locus_tag="R8G18_001754"
     CDS             complement(<1..643)
                     /locus_tag="R8G18_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HEE9795473.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="R8G18_001755"
     CDS             complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="R8G18_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HEE9795474.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="R8G18_001756"
     CDS             complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="R8G18_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HEE9795475.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="R8G18_001757"
     CDS             complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="R8G18_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HEE9795476.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="R8G18_001758"
     CDS             complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="R8G18_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HEE9795477.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="R8G18_001759"
     CDS             complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="R8G18_001759"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEE9795478.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7180..8415
                     /gene="moeA"
                     /locus_tag="R8G18_001760"
     CDS             7180..8415
                     /gene="moeA"
                     /locus_tag="R8G18_001760"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEE9795479.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8415..9164
                     /gene="moeB"
                     /locus_tag="R8G18_001761"
     CDS             8415..9164
                     /gene="moeB"
                     /locus_tag="R8G18_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEE9795480.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="R8G18_001762"
     CDS             complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="R8G18_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEE9795481.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10136..11035
                     /locus_tag="R8G18_001763"
     CDS             10136..11035
                     /locus_tag="R8G18_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEE9795482.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11041..13473
                     /locus_tag="R8G18_001764"
     CDS             11041..13473
                     /locus_tag="R8G18_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEE9795483.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13619..14434
                     /gene="ybiV"
                     /locus_tag="R8G18_001765"
     CDS             13619..14434
                     /gene="ybiV"
                     /locus_tag="R8G18_001765"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HEE9795484.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14586..15851
                     /locus_tag="R8G18_001766"
     CDS             14586..15851
                     /locus_tag="R8G18_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HEE9795485.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15818..16978)
                     /locus_tag="R8G18_001767"
     CDS             complement(15818..16978)
                     /locus_tag="R8G18_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEE9795486.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17216..18517
                     /locus_tag="R8G18_001768"
     CDS             17216..18517
                     /locus_tag="R8G18_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9795487.1"
                     /translation="MSQGINNNMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18532..20895
                     /locus_tag="R8G18_001769"
     CDS             18532..20895
                     /locus_tag="R8G18_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEE9795488.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21075..22670)
                     /locus_tag="R8G18_001770"
     CDS             complement(21075..22670)
                     /locus_tag="R8G18_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEE9795489.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22889..23809
                     /gene="ldtB"
                     /locus_tag="R8G18_001771"
     CDS             22889..23809
                     /gene="ldtB"
                     /locus_tag="R8G18_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEE9795490.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23868..24986)
                     /locus_tag="R8G18_001772"
     CDS             complement(23868..24986)
                     /locus_tag="R8G18_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HEE9795491.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="R8G18_001773"
     CDS             complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="R8G18_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HEE9795492.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25636..25764
                     /gene="mntS"
                     /locus_tag="R8G18_001774"
     CDS             25636..25764
                     /gene="mntS"
                     /locus_tag="R8G18_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HEE9795493.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26036..27619
                     /gene="opgE"
                     /locus_tag="R8G18_001775"
     CDS             26036..27619
                     /gene="opgE"
                     /locus_tag="R8G18_001775"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEE9795494.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27668..28183)
                     /gene="ompX"
                     /locus_tag="R8G18_001776"
     CDS             complement(27668..28183)
                     /gene="ompX"
                     /locus_tag="R8G18_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HEE9795495.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28236..28301)
                     /locus_tag="R8G18_001777"
     CDS             complement(28236..28301)
                     /locus_tag="R8G18_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795496.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28536..29423
                     /gene="rhtA"
                     /locus_tag="R8G18_001778"
     CDS             28536..29423
                     /gene="rhtA"
                     /locus_tag="R8G18_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HEE9795497.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29722..30225
                     /gene="dps"
                     /locus_tag="R8G18_001779"
                     /gene_synonym="pexB"
     CDS             29722..30225
                     /gene="dps"
                     /locus_tag="R8G18_001779"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEE9795498.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30629..31375
                     /gene="glnH"
                     /locus_tag="R8G18_001780"
     CDS             30629..31375
                     /gene="glnH"
                     /locus_tag="R8G18_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HEE9795499.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31514..32173
                     /gene="glnP"
                     /locus_tag="R8G18_001781"
     CDS             31514..32173
                     /gene="glnP"
                     /locus_tag="R8G18_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HEE9795500.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32170..32892
                     /gene="glnQ"
                     /locus_tag="R8G18_001782"
     CDS             32170..32892
                     /gene="glnQ"
                     /locus_tag="R8G18_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HEE9795501.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33009..35234
                     /gene="ybiO"
                     /locus_tag="R8G18_001783"
     CDS             33009..35234
                     /gene="ybiO"
                     /locus_tag="R8G18_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HEE9795502.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35231..36157)
                     /gene="rlmF"
                     /locus_tag="R8G18_001784"
     CDS             complement(35231..36157)
                     /gene="rlmF"
                     /locus_tag="R8G18_001784"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HEE9795503.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36433..36693
                     /locus_tag="R8G18_001785"
     CDS             36433..36693
                     /locus_tag="R8G18_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEE9795504.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36958..>37258
                     /locus_tag="R8G18_001786"
     CDS             36958..>37258
                     /locus_tag="R8G18_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEE9795505.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9035 a        10059 c         9336 g         8828 t
ORIGIN      
        1 acaaatcgtg atctttgccg ttgatacgta gcgtaaaaga gatacatttt ttacccggac
       61 gatcaaagca attgtatgcc ggattccagc tgttgttaga gtcatctgaa gccaccgacc
      121 acggtcgttt cgatgggtta aaatcggcaa aaataacccg ccctgactcg tcgacaagcg
      181 gtccatttac ttgttcctga tgataaacaa cgattttatc attcgccctc cgcgaaaaga
      241 gataatattt attatcatca agtaccagtc ccaccgagtc taaaaatggc atcatttcca
      301 ttttcagaat cacggcgcgt tgaatctcct ctgtattttt taataaatac agtgagtcct
      361 ttaatgtcag ggccgaacgt tcaatatctt taaagaaata gccaacgttt ttctgaataa
      421 gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga tagttaatga
      481 tgaaaaaaag accaataaat gacagtgctg ccaataaaaa taaaattatc agccttatct
      541 ttatcttttc gtataaactc agcatgttaa tacatttaac aaaataacta tctgaacgct
      601 gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa
      661 aatcagcaga aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct
      721 ttcaagcaat attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac
      781 catcgcccaa caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaaaacag
      841 cgacatgcgg cgcgataacc atatccgctc gtgcctcatt gagcattgct ccccactctg
      901 gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct ggcggcagag ataatcgagg
      961 tgccaatgcg catggtgaaa aacaccacga tagacgagac ggtcccaggc aagatatgac
     1021 gcaataaaat ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata aaggtttgct
     1081 gtttcaacac cagcgtgttg ccgcgcacca gtcgggcaaa cgcggggatg gaaaaaatgg
     1141 cgactgcgat aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca
     1201 gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg catgatcagc cgatcccacc
     1261 agccttcata atatccagcc aacaagccca gcaacgtccc gatcgccgca ccgataaaca
     1321 cggcaaacac gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt
     1381 cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt
     1441 cataatcaaa ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca
     1501 caatcaataa aataacgaat aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc
     1561 gccagaattc atgccacggt gtacgcacct ggtcaggttt gaccagtggc atggcgttta
     1621 acaccgcctg acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt
     1681 aatggcggcg taaagcacat ccaccactaa gttgataaga ataaattcca gcgagaaaag
     1741 cagaatttcc gcctgaatca ccgggtaatc gcgcatttct acggagtcaa cgagtaagcg
     1801 tccaagtccg ggccagttga aaactttctc caccacgata gaaccaccga gcaaaaagcc
     1861 aaactgtaag cccatcatgg tcactaccgg gatcatcgcg ttacgtagcc cgtgtttgag
     1921 gacaacccag gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa
     1981 aacatcgaca aacgacgcgc gggtaaagcg cgccatcacg gctgccactg ccgcgccgag
     2041 cgtcagggag ggtaaaatgt agtgctgcca actgtctgct cccacggtag gcagccagcc
     2101 cagttcaacg gagaatacct gaattaaaag catccccagc gcaaatgccg gaaacgagat
     2161 ccccgacacc gcaatggtca tactcaatcg atccggccaa cgattacgcc agacggcggc
     2221 gataattccc gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca
     2281 cagcgttggc ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga
     2341 caggccaaaa tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg
     2401 ctgatccaac cccagctgct gacgcaccag ttctataacc tgcgcatcag cttcgagccc
     2461 ggcaatcaat cgcgccggat cgccgggcag catatgcaca aataaaaaca ccagcaccga
     2521 gacgataaac agcgtcggaa tcaaccccag taagcgtttg ataacgtaat taagcattcc
     2581 actccctgcg ttgcttattg caaatccgcg tcttcaaagc tgaagccggt gtctggcatg
     2641 atccaaaaac cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg
     2701 atccacggcg actcttgcca gatgatatcc tgcgccgcct tatacaagcg ggtcttttcc
     2761 gccggatcat tagttttcag tgcctgagcc aggaagtcgt ccacctgttt attgctgtaa
     2821 aatgcggtat taaacagcgt cggtggccag ttttgcgagg cgaacagtgg cgatagcgcc
     2881 cagtccgctt cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct
     2941 ttttgccctt taccttcaac ttctgccgcc cgctgtccgg catccatcgc ggtcacctgg
     3001 gctttaatcc cgacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg
     3061 ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc
     3121 agtaattcgc gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg
     3181 atacttggcg gtaccacacc agtagctggc gttgcatagc ccgcaaaggc aactttcacc
     3241 agcgccggac ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc
     3301 ttttgcgtca cgttcatact gatataacgc tgcataattg acggactggc catcaactcg
     3361 atatttttgt ttttctccag cagtgtggcc tgctcgtagg gaatggggaa agcaaactgc
     3421 gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt
     3481 atgctgtcca gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca
     3541 aaatcggtct gattccaggt atccagttca tacggtccgg ttcccaccgg atgaaaacca
     3601 atctccttgc catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc cggatgggca
     3661 agaatattaa taaacgctga gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg
     3721 atcgcctccg ttttggcaat attcttatac aggttatagc gtttaagatg attcgccgga
     3781 tcgctggccc ggtccagatt cgcttttacc gccactgcgt tgaaatcggt gccatcctgg
     3841 aatttaatgc cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga aacggtataa
     3901 ctctccgcca gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa
     3961 aacgatttcg ctacggcctg agataacgtg tcattcgcgt catacggatc gagcgtggtg
     4021 aaattcgatc ctaccgccac caccacatct ttggcagcga atgcacaaga tgccatcaac
     4081 gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta cagctcttgc cattttattc
     4141 tccgcctgaa tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg
     4201 ccccggcccg acgcattgca acgagacggc tgccacctct tcgccacgca gatgaatatt
     4261 gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac
     4321 cggaactgcc gccagtaatt tacgcgtata aggatgctgc gggttttcga agaccgcgcg
     4381 ccgtggacca atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg
     4441 ctctaccacg gccatatcgt gggagataaa cagatacgca atgccgaaat cacgctggag
     4501 atcgagcaac aagttgataa tctgcccgcg aatagaaaca tccagcgccg aaacggcttc
     4561 gtcggcaatg atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg
     4621 ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg
     4681 ctccagcaac cacgcaacgc gtgcagccgc gtctttacct ggcaataaac cgtgtacacg
     4741 cagcggttcg ataatcgaat caccgatggt ctgacgtggg tccagcgaag cgtaagggtc
     4801 ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt ttgccgggtg acaaggtatc
     4861 gattctctga ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc
     4921 ccgcccggta gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg
     4981 ccagagatca aaactgactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa
     5041 accgctgcgc aaagggaaac gggtgacaag attacgcact cgtaaaacag gttcgccatc
     5101 caccaccgtt ttctgctcga tggggggggc ctgtttcgct ggatgttcaa gcgatatcaa
     5161 cgggaaacgt cggggataat ccaacccttt catcgcacca agttgcggaa cagcagctaa
     5221 cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc
     5281 caccgcctcg ccctgataca tcaccagtac ccgatcggca atctctgcca ccacgcccat
     5341 atcgtgagtg ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg
     5401 caggatctgc gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac
     5461 cgccgggcgg catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag
     5521 ttgatgcgga taacgtgaaa gaatggtttg tgcctcagga atgcgtacct gatccagcat
     5581 ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaattgattc
     5641 ggcaatctgt tcacccacag taaataccgg gttcagcgat gtcatcggct cctgaaatat
     5701 catcgccata tccgcaccgc gaacatggcg catttgtgca gcgctctgct cgctaagttc
     5761 aatcacttcg cgactgcgcc gctgcaacag cattttatcg cactgtacta aaccgcccgc
     5821 ctgttccaac aggcgcatca atgccaacgc agtcactgac ttaccggagc cggattcgcc
     5881 aacaattgcc agcgtctcac cgcgttgcag actaaaagag agattgcgga ccgcagctat
     5941 tttctgctgg tcctgcataa aggcaatatt cagattttca accgccagca cattaccggc
     6001 atcaagttca tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg
     6061 gcgtatcgcc tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga
     6121 cattcccttc atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt
     6181 ccattactac ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg
     6241 cgatgtcata tgccgccagc gcgcggatga agacttcgcc cgtgccgata caagaaaccg
     6301 ccacactggc gttattggcg tagcatccgg cacccactaa gggactatcg ccaactcgtc
     6361 cgggtaattt attggtcatt ccgcctgtgg acgtggctgc cgccaaattg ccgtctaaat
     6421 ccaacgccac ggcccccacg gtgcccattt tctgtttttc atccagtggc gcaccgctat
     6481 ggtcgaggac ggttgcccct tccttgcgcg ctgccagtag ttgttcataa cgcaaaggcg
     6541 tggagaaaat ctccggcgag acgcgctcca tgccacgagc aaacgcaaaa ttttctgccc
     6601 cttcgccaat catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg
     6661 gattacgcag atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca
     6721 cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa
     6781 acagtggaca ctcttccagc agacgcaccg cttccgtcac cacatccagc gcactttcgc
     6841 ccgcttccag cattttctgc ccggtttcaa caatggcaga caacgcctca atgtagcgta
     6901 attcctgttg cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa
     6961 tgactgcttt gcccatagta ttcgtccatt aagggtggct cgccactttt gctataaata
     7021 tcagaatttt tatgaattac tcattcaatt tttgaatata gaaagatgga tatggcatgt
     7081 aaaggcagga gccaacgcta cgcccgaggt ggcatacagt ctgtggcgtt tttctgacat
     7141 aataggcaaa ttcgattttg cctccgcagg agtgttttca tggaatttac caccggattg
     7201 atgtcgctcg acaccgcgct taatgagatg ctttctcgcg tcaccccact gaccgcccag
     7261 gaaacgctgc cactggtaca gtgttttggt cgtattctgg cgagcgatgt cgtttcgccg
     7321 cttgatgttc cggggtttga taactccgca atggacggct acgcggtgcg tttagccgat
     7381 attgcctccg ggcaaccgct gcccgttgcc ggtaaatcct ttgccggtca gccgtatcat
     7441 ggtgaatggc ctgcgggcac ctgcattcgt attatgaccg gtgcgccggt gccggaaggc
     7501 tgcgaagcgg tggtgatgca ggagcagact gaacaaacgg acaatggcgt gcgttttact
     7561 gctgaagtgc gtagcgggca aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg
     7621 gttgttttcc cggcgggaac tcgcctgact accgcagagc tgccagtaat tgcttcactg
     7681 gggattgccg aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat
     7741 gaactccagt tgcccggtca gccgctgggc gacggccaaa tctacgacac caaccgtctc
     7801 gccgtacacc tgatgttaga acagctgggt tgcgaggtaa tcaacttagg gattatccgc
     7861 gacgatcccc atgccctgcg cgccgcattt attgaagccg acagccaggc ggatgtagtg
     7921 atcagttccg gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat tcttgaagag
     7981 ctgggggaga tcgccttctg gaagctggcg attaaaccag gtaaaccgtt cgcgttcggt
     8041 aaactcagca atagctggtt ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc
     8101 ttctatcaac tggtacagcc tttgctggca aaactaagcg gcaacaccgc cagcggcctg
     8161 cccgcgcgcc agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat
     8221 ttccagcgcg gcgtgctgca acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga
     8281 catcagggtt cacatatatt tagctccttt agtctcggca actgctttat cgtgctggaa
     8341 cgcgatcgcg gcaatgtgga cgtgggcgaa tgggtggaag tagaaccgtt taacgcgttg
     8401 ttcggaggcc tgtaatggcg gaactcagcg atcaggagat gctgcgctac aaccggcaaa
     8461 tcattctgcg cggttttgat tttgacggtc aggaggcgct aaaagattct cgtgtgctga
     8521 tcgtcggcct cggtggactc ggctgcgcag cttcgctgta tctggcaagc gccggtgtcg
     8581 gcaacctgac gctgctcgac ttcgacacgg tttcgctctc gaatctgcaa cgccagacac
     8641 tgcacagtga tgccacggtc gggcaaccga aggtggaatc cgcccgtgac gccctgacgc
     8701 ggatcaaccc acatatcgcg attacgccag tcaatgcact gctagatgac gcagaacttg
     8761 cagcattgat tgctgaacac gatctggtgc tcgactgtac ggataacgtt gcggtacgta
     8821 atcaactgaa cgcaggctgt tttgccgcga aggtgccgct ggtttccggc gcggcaattc
     8881 gtatggaagg gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc
     8941 ttagccgttt gtttggcgag aacgcattaa cctgcgtaga agcaggcgtg atggcaccgt
     9001 tgatcggcgt aattggttcg ttgcaggcaa tggaagcgat caaactgctg gcaggttatg
     9061 gcaaaccgac cagcgggaaa atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa
     9121 tgaaactgat gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc
     9181 tgataagacg cgacaagcgt cgcatcaggc atttgctcca gacgccagat gcggcgtgaa
     9241 cgccttatct ggcctacggt tcgggcacag atttccaagc gcggcaggca tcggtgcact
     9301 ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc
     9361 aaacgctccc tgccagtcct gctcaaactt cgccacagcg gcttccaccg ccggatagct
     9421 aatcatctgt tgtgccacat ccagtggcag agtaattgat tcacatcctg ccagtaagca
     9481 gtccagcgcc tgacgcgggg ttttgaaact cgctgccagc actttcgcct gcggcgcatg
     9541 cattttcaat aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc
     9601 aatacgatta acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcaacccttg
     9661 tgctgcgcca tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat
     9721 agctgccagc ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg
     9781 caatttacgt gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag
     9841 acgcccctga ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt
     9901 acccgcggcg ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag
     9961 cgccttcacc gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat
    10021 gtactttttg tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc
    10081 aatctaactt tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat
    10141 tttcaatatt cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct
    10201 taaaggctgt tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca
    10261 ggatctgctg tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc
    10321 gccggaagtg attgagcgcg cgctgaatgg tttgcttatt catcgggaaa agttaacccc
    10381 ggagcatctg acggcgttaa ccgactgctg tccgacacag gcattaaccg tgtgtggtga
    10441 agtgaaaagc gttgaggaga tcatgacgac cgttctgcgc gataaaccgt tttacgatcg
    10501 cagcggcggc ggtttaacgc tttcgggtgg tgagcccttt atgcagccgg aaatggcgat
    10561 ggcgctactg caagccagcc acgaggcagg cattcatact gcggtagaaa cctgtctgca
    10621 tgtgccgtgg aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt
    10681 aaaacacgtt gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccagagtgct
    10741 ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat
    10801 tcagggcttt aatgccgacg aaacctctgt aaaagctatt accgattttg ccgccgacga
    10861 gctgcacgtt ggcgaaattc atttcctgcc ctaccacacg ctaggcatca acaaatatca
    10921 cttacttaat ctgccctatg acgccccgga aaaaccgctt gatgcgccag aactgctcga
    10981 ctttgcccag cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc
    11041 atgaccacac tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg
    11101 gtgcatattg tgaaaccgcc agtctgtacc gagcgcgcgc agcactatac cgagatgtat
    11161 caacaacatc tcgataagcc gatcccggta cgtcgcgcgc tggcactggc gcatcacctg
    11221 gcgaatcgca ccatctggat caaacacgat gagttgatca ttggcaacca ggcaagcgaa
    11281 gttcgcgccg cgccgatctt cccggaatat actgtctcgt ggatcgaaaa agagattgat
    11341 gatctggcag atcgtcccgg tgctggcttt gcggtgagcg aagagaacaa acgcgttctg
    11401 catgaagtgt gcccgtggtg gcgcggtcag accgtacagg atcgctgcta cggcatgttt
    11461 accgatgagc aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc
    11521 tccggcgatg cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt
    11581 ctgcgcgaga aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg
    11641 cacggcgaac agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt
    11701 gaacgttttg cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc
    11761 gatgaactgc tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact
    11821 ttctggcagg cgctgcaact gtgttacttc atccaattga tattgcagat cgaatctaac
    11881 ggtcactcgg tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgcgac
    11941 gttgaactca accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg
    12001 ctgaaactgc tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga
    12061 agtcctctgt atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg
    12121 gacgcggtga atccactctc ttacgctatc ctcgaatcct gcggtcgcct gcgttccact
    12181 cagcctaacc tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc
    12241 gtacaggtga tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc
    12301 ccggaattta ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt
    12361 tgtatcgaaa ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc
    12421 aacttcgccc gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa
    12481 gtgttcctgc cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta
    12541 atggacgcgt gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat
    12601 gtcgtcgaca ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat
    12661 gactgtattg agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct
    12721 ggcctgcagg ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta
    12781 tttgaacagg gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat
    12841 ggcctgactc atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac
    12901 gacgatgata ccgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg
    12961 aaacagtacc ataacccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt
    13021 acctcgtcta tctccgctaa cgtaccgttt ggcgcgcaaa ctatggcaac accggacggg
    13081 cgtaaagccc acaccccgct ggcagaaggc gcaagcccgg cctccggtac tgaccatctt
    13141 ggccctactg cggtcattgg ctcagtgggt aaactgccta cggcagcgat tctcggcggc
    13201 gtgttgctca accagaaact gaatccggca acgctggaga acgaatctga caagcagaaa
    13261 ctgatgatcc tgctgcgtac cttctttgaa gtgcataaag gctggcatat tcagtacaac
    13321 atcgtttccc gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg
    13381 gtagtgcgtg tcgcgggcta ttccgccttc ttcaccgcgc tctctccaga cgcacaggac
    13441 gatatcatcg cccgtactga acatatgctg taatctcctc ggcccggcgg cgtcgtcgcc
    13501 gggctaaatc attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc
    13561 tctgcgtcac attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat
    13621 gagcgtaaaa gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta
    13681 caaccaacca cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt
    13741 tgtcgccagc ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga
    13801 gatctctttt gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca
    13861 cggcgaactg acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca
    13921 actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc
    13981 atttgtcgca ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga
    14041 gattgacgac gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt
    14101 gatcgacaaa ctgcacatag cgctcaatgg cattatgaaa cccgtcacca gtggttttgg
    14161 ctttatcgac ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa
    14221 acgctgggat ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga
    14281 gatgctgaaa atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca
    14341 aatcgcccgt tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc
    14401 ggtgctggat aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg
    14461 gttttccctt ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta
    14521 tttataactt atattgtcgc atgcaaatca gaatggtttc gaatgaaagt taatgagagg
    14581 tggttatggc ttctactttt accagcgaca cattgcctgc cgatcacaaa gcagctatcc
    14641 gtcagatgaa gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc
    14701 taagcgatga cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg
    14761 ccgtctggcc ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc
    14821 gcgaacagat taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag
    14881 cgctaggctg ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct
    14941 acaaaggccc cggcgataat ttcttcggga cgctcagcgc ttcacgtccc gagatttacc
    15001 ccatctactg gtcgcaggcg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc
    15061 agtcgtttct caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg
    15121 atgtgagcgt catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag
    15181 gtcttggagc gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc
    15241 gcgttttcgc caacgtcttt aatggcaatc tggcgcaata tgatccctgg catgcggcac
    15301 atcgtacgga agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga
    15361 cattccaagg ctggacagcg ctctctaata tgctgcctgg tcaggggctg ctgcacgttg
    15421 tgcccattcc tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg
    15481 aggatgaact gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc
    15541 cactgttaat tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt
    15601 ggcactgcga cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg
    15661 tgatgtacat tcctgcggca ccaatgtgcg agaaaaatct tgcctacgcg cacaaggtga
    15721 aggccgcact ggaaaaaggc gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa
    15781 actgggaagg acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggaa
    15841 tcgacagctg agtgtcgtac agcccttccc gttccacggc ggtgttccct tttcctgggc
    15901 gaacccgccg cactgactcc cgcaccgcca atcgtccgtg cagcagcagc gagcccaccg
    15961 gagcatccgg gcgaatcagc cgctgttgca acatttgcac cgcttcattg cctagctcat
    16021 cgcgtggcac atgcacagcc gtcaggggaa catcctcgat tgccgcaagg ttaaagccgt
    16081 cgatactcat caccgagaca tcctgtggta cgcgcagacc acgacgatgt agcgcattta
    16141 ccgttcccgc cgccataaaa tccccgccga ccagaaacgc cgtcggcaac gtcttgccat
    16201 tggcggcatc cagccacgct ccgacctttt cttccgcttc tttagcgcta aaactgctga
    16261 tattcagcaa atgacggtca tggtgaaagg ccagattatg ctgacgccat gattctttaa
    16321 tccctgcaaa ccgctgctcc atagtgtagc ggcgcaggca catcacattc atcacctccc
    16381 ggtgacccat ttcaaacaga tagcgggcgg cgaacgcgcc aattaaccga tgatccggcg
    16441 caatcgccgg gaggcgcatt cgttcatcac ggcagttgat cagcacacaa ggcttagcga
    16501 gatcggcggc cagatcatgg atgtgcggat cgtcaatccc cagcaggatc gctgcctgcg
    16561 tatctgcttc attcatccgc gagagaaaga gcgcgggatc gctgtcaaac tcctccagcg
    16621 cgcagtaacg cagccgcact tcatgcggcg ataacgcttt gctgatgctc tggatcacac
    16681 ggtaataaaa gatatcagat cgttggtcaa aagcgcgttg cggcgcgaaa ataatcaggt
    16741 tgttcagcaa catacgacct gctgccatgc catccatcac gcccaactca cgcgcgcagg
    16801 ccagcacctg ttcgcgagcg cgtgcactgg tattcgcttt gccagccagc acccgcgaaa
    16861 ccgtactcgg cgatagcccg gtacgtgcgg cgatatcggc gattttcagc ttcttgtcca
    16921 ttttgtgatc tgcctctaat tcggaaatga cagaattttc atgagcaaaa aagcccattc
    16981 tgtctggcat taaatccatt tcagtaatgt atatcagtca ctcatggaaa aacgtgcaca
    17041 aaacccgctt tctttcgctt ttagggtctc ttactgtgaa ttggtagaac aacttccata
    17101 caaaaataat ggaaaaacga acaataaaga ctttatttat caaaaagtta aaagtggtgt
    17161 aacaactcca ttctgacgtt gccgcacaaa ccctatacca ttctgtggag tgcatatgag
    17221 tcagggtatc aataacaata tgactgccag taaaccgcgc catatcgtca aaaatctgcg
    17281 ttggtggata ctggtccttt ttttgctcgg tgttactgtc aactacatca cgcgtaactc
    17341 gctgggtatt ctcgcccccg aactgaaaag cagcctaggg attaccaccg aacaatattc
    17401 ctggattgta ggtgctttcc agctcgccta taccctattc caaccgttgt gcggatggct
    17461 gattgacgtc attggcctca aactgggctt tatgatctgc gcgggactgt gggcactggc
    17521 ctgtctgatg catgcggggg cgggaaactg ggtacatctg gcgctgttgc gcttctttat
    17581 gggcggtgcg gaagccgcgg caaccccagc aaacgccaaa accatcggtg aatggtttcc
    17641 aaaatcagaa cgcccgatcg ccgcaggctg ggcaggcgtc ggtttttcca tcggtgccat
    17701 gctggcccca ccgattatct attttgccca cgcttcattc ggctggcagg gtgcatttat
    17761 gttcactggc atcctggcga tgctgtgggt ggtgctgtgg tgggcgttct atcacagccc
    17821 ggataagcat cctaacctca gcaaagccga gctggacttt attcgccagg ataacgaagc
    17881 gcccccggtc aagttaccct ttctgaccgc actgaaaacc gtgggcaaaa ataaacgctt
    17941 ttacggtatc gctatcccgg cctttatggc agaaccggcg tgggcggtga tgagtttctg
    18001 ggtgcctcta tacctgtcca aaacctatgg catggacctg aagcagatcg cattattcgc
    18061 ctggttgccg ttcctcgccg ctgatcttgg cagtattgcc agcggctatc tgaccaaact
    18121 ttacacccgc ctgtttgggc tgagtcgtgt gaactccgtt gtcgcatcat cggtcagtgg
    18181 cgcattcttg atggtttcgc tggcggtgat ggcctttacc aataacccgt atatcgccat
    18241 cgtccttatc tccatcggcg gcttcggtca ccagatcatc tcctgcatgc tgagcgcact
    18301 ggtggtggaa tccttcgaca aaggtcagat ggccactgtt aacggcatgc gcggttctgc
    18361 cgcgtggatc gcaagtttcc tgttctccct gattatcggg gttaccgctg acaaaatcgg
    18421 ctttaacccc ctgtttatcg ccatgggctt ctttgacctg attggcgctt tcttcctggt
    18481 cacctttatt gctgaacgtc gcgcaaaacg cgcctgagag tggatgtaaa catgaaaacg
    18541 ctgaaaaact ggaccctgca caaacagtct gcccaccacg ttgaactgct ggtcgatggc
    18601 cagcatatcc tgtgcctgta cgtgctggaa gaaaatctgt tccgcgtgct gttaaaacgc
    18661 aaaggcgagc tggcgctaga tcgcacctgg agcattgctc cggcgcagga tgtgccatgg
    18721 gaagggcgca gccgcgaaga tctcagcggc tttagcctgc ccgcgtggac agtgatccag
    18781 cacgacgaca ccgtggttat cgccaccgag tcactgcgtg caaccgttca ccagccgctg
    18841 tggctggagt ggcactatcg taacaacgaa ggcgaatggc tgccgttggt gaatgaccgc
    18901 ccgaccagcg cttatctgct gaatgcccac ggcgatggcg tggcacacta tcagagccgt
    18961 cgtaaagatg aacgctttta tggcctcgga gataaagccg gcgatctgca acgcaacggt
    19021 aaacgttacg agatgcgtaa cctcgacgcg atgggctaca acgccgttag tactgacccg
    19081 ctgtacaaac acattccatt caccatcacc cgccgcgatg acattagctt cggcctgttt
    19141 tatgacaacc tcagcagctg ctggctggat ctgggcaacg agatcgacaa ctaccacacc
    19201 gcctatcgcc gctggcaggc ggaagcgggt gatatcgact actacctgtt taccggcgaa
    19261 cgcgtacttg atatcaccaa agccttcgta cgcctgaccg gaaaaaccct gttcggaccg
    19321 aaatggagcc tgggctacag cggttcaact atgcactaca ccgatgcgcc ggacgcacaa
    19381 aatcagttga tgaactttat ccgcctgtgt gaagaacatg caattccctg cgattcgttc
    19441 cagctctctt ccggttatac ctcaattaac ggcaaacgct atgtgttcaa ctggaactac
    19501 gacaaagtgc cgcagccgaa agtgatgagc caggcatttc acaatgctgg tttgcacctg
    19561 gccgccaata tcaagccgtg cctgttgcag gatcacccac gctataacga agtggcagaa
    19621 agaggactgt tcatccgtga ttcagaaaca gatgaaccag aacgttccag cttctgggat
    19681 gacgaaggtt ctaacctcga ctttactaat ccgcagacca tccagtggtg gcaggaaggc
    19741 gtgaccacac agcttctgga gatgggtatc gactccacct ggaacgacaa caacgaattc
    19801 gaagtatggg acggtgaagc acgctgtcac ggctttggtc agacgatcgc gatcaaacat
    19861 attcgcccag tgatgccact gctgatgatg cgagcctcga tggaagccca gcagcgtttc
    19921 gctccggaaa aacgtccgta cttgatctcc cgctccggct gcgcgggaat gcagcgctac
    19981 gtgcagacct ggagtggcga taaccgcact agctgggaca ctctgcgcta caacacccgc
    20041 atgggtctgg ggatgagcct ctccgggctg tataacctcg gtcacgacgt cggcggtttc
    20101 tccggtgata agccggaccc ggaactgttt gtacgctggg tacaaaacgg cgtgatgcat
    20161 ccgcgcttta cgattcactc atggaacgat gaccataccg taaacgaacc gtggatgtat
    20221 ccgggcgtca ctccggcgat ccgcagcgct attgagttgc gctatcgcct gctgccctac
    20281 ttttataccc tgatgtggca ggcatacgcc gatgatgaac caatgctacg tccgactttc
    20341 cttgaccacg agcacgacgt tcagactttt gaagagtgtg atgacttcct gcttggtcgc
    20401 gatattttgg ttgccagcgt cgtagagcaa ggcgagcgcc agcgccgtgt atggctgcca
    20461 gataacgaaa ctggctggta cgatttctac aacggcgaat ggttcagtgg cggtcagtgg
    20521 atcaccctcg acgcaccgct ggagaaactg ccgctgatgg ttcgtgccgg ggctggtcta
    20581 ccgctcagca aacgcatcac ctatgtgagc gcagaacaag acgatacccg cgaactgaaa
    20641 ctgttcccgc tgaaaggtgt gggcacaacg tccggcctgc tgtttgaaga cgatggcgaa
    20701 agctggggct accaaactgg taatgcgctt tgggtggaat gggaaatggt ttgcgatggg
    20761 gcgaccatca acctgaaagt caacgcgcgc ggggattatc gtcctgcatg gagtgcactg
    20821 aaagtgtcat taccattgga agaaaaacgc acgctgctgg tcaatggtgt tgaagggagt
    20881 gaatggatgc gttaaatcat taccggggcc gggaggcgac tgcaccttac ccggtccagc
    20941 catcgaatcc tttacctcag tatttcacgc tgcaggaagg cagcaaacct gtaaaccccg
    21001 ggagcttaaa taagtaagtg accggggtga acaggtgaag ccaatgcatc tgccgcatga
    21061 aagacggcgg ggaattaagc gtcgatccct ttactacgca ggtaatcttc gtaattaccg
    21121 ctaaagtcga tcgcgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg
    21181 aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc
    21241 agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc
    21301 ggcttctgca tcattaactt accaaacagc atccgccctt tttccccacc ggaaagcact
    21361 ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc
    21421 gcctgctcgt catcgccttc ctgcttccac tgactcatcc attcgaacac ggtcagatca
    21481 ttttcaaact catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat
    21541 tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg ttttcagcag cgttgattta
    21601 ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga
    21661 tttttaaaca gtggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta
    21721 cggaacagtt tcttatcctg ttcaaaacgg atgaacgggt tctgacggct ggaggctttc
    21781 acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc
    21841 gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg cgctttcttc
    21901 ttggcgttat cggccagcag acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg
    21961 tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc
    22021 atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc
    22081 acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg
    22141 agaatatccg gatcagcaaa cagcgcctgc gccagaagca cacgcagttt ccagccagga
    22201 gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacagt
    22261 tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttaac ttccagatcg
    22321 gccactttat agccgtcttc ctcactcatt tccggcaaag catagatgcg gtcacgctcc
    22381 tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct
    22441 tcaaaggcaa actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg
    22501 ttacccaaag tcggctctaa gtcgccgccg aggatcttca taaaggtgga tttaccacta
    22561 ccgttcgcgc caatcaggcc gtaacggttg ccgccgccga atttgacgga aatgttttca
    22621 aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc
    22681 tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct
    22741 cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc
    22801 actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg
    22861 gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc
    22921 ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac
    22981 cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca
    23041 gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa
    23101 cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct
    23161 gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta
    23221 ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg
    23281 caaagatacg cctatcaact ggaccaccaa agttgagcgt aagaaagcag gcccgacctg
    23341 gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt
    23401 cgttccggca ggtccggata acccgatggg gctgtatgca ctctacatcg gtcgcctgta
    23461 cgctatccat ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt
    23521 gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt
    23581 acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga
    23641 agtccataac ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa ttgtgccaat
    23701 taccctgacg aagagcgtgc aaacagtgac cggtcagcca gatgttgacc aggttgttct
    23761 tgatgaagcg attaaaaacc gctccgggat gccggttcgt ctgaattaat cttcaaaact
    23821 taaagcaaaa ggcggactca taatccgcct tttttatttg ccagacccta gttggccggg
    23881 agtataacta acaaaacata tcccaccaac gccgcccaca acagcatcgg tattgaatag
    23941 agatggaaac gccaccagat gcggcgatcg ttcgccatac gtagcgcaat caaatttgcc
    24001 agcgatccgg gtaataaccc aaagccacct acgtttaccg cccataccag taataaagac
    24061 ggcggcacat agttcagcaa caatatggta ctcggcacgt tactgatcac ctgcgataaa
    24121 ccgattgccg ttaaccataa cccgggttca gatagatgac tcacgttacc caacacgcct
    24181 tgcaacgctg gaagctgggt cagtaaatgg acgtcgataa acatcgccat aaacaccagc
    24241 agcagcgtcc agtccacact gagcaccacg cgacgtgcca gcagcgcaaa gcctgccgcc
    24301 acaatcacca gtccccacag ctcttgtttg aactccagcg ccgtcagaaa gacgatatac
    24361 agccccagac aactccacac cagccgcggt ttccactccg gtgtttgcac ccccgtatgg
    24421 tattgcagcg cctttccagg gaaacaacac cagcacagga gcaggagcgt cagcatcatt
    24481 gcgccagcca gcggtgccat ttgggcaata aatccggcaa acgaaagacc agaacgtccc
    24541 cagataagaa tattttgcgg gttgccaatt ggcgtcagta gcgaaccagc gttgactgcc
    24601 agcgcctcaa aaataatcag ccgattaacc gggatctcac acagtctttt tagcgtgata
    24661 gtcagcggaa caacaataaa cagcgcgaca tcgttggtca gaaaggtaga aagcagcgcc
    24721 gccgccagca ccataaacat cgccagccga cgctccgtag caaagcggcg caccattttg
    24781 cgccccagca catcaaaata accgcttaac tccacacctt tggtcagcag catcaggccg
    24841 cttaaggtga tgatggtgtg ccagtcgata gcagcaggcc aggatttcgg tgcaaagggc
    24901 acgaaaaagc ttaatccgat accaacaaga attaataact gaaaaaaacg atcgccttgc
    24961 agcgtgcgta aaaaaggcag gctcatttgg caccgtgttt ctgggtaaac aaacgaaaag
    25021 cgtcgagcgt ctcttcacta acatggtgct ccatgccttc cgcgtcgcga cgggcgattt
    25081 ccggactgac gcccaacacc aacaagaaat tttcgactat ctgatgtcgc tcgcggcttt
    25141 cctgcgccag cttctctcct tctgccgtta aaaacacgcc acgccagggg atcatttcaa
    25201 tcagccccat ggttgccagc cgcttaagca ttttagccac cgtcggttgc gaaactccca
    25261 gacgagcagc catgtccacc tgacgagctt cccccacttc cctgatcaag tcagaaatca
    25321 gctcaacgta atcatcaata agctcgcgcc gatgcgcctc tctgacctgg cggaaccctt
    25381 caacgtgctc ttccacgttc actaactgcg tcactttttt tgctgttggc gtacctgcgc
    25441 gacgactcat tgtgcttcct cattatggtg acgcatacat agcgttctca tttcgtgcgc
    25501 acattgtaaa ccagagttgc gaaggtacaa aaaattaacg ttttagcaat agctacataa
    25561 tatagcctgt gctatatctg tatgtaatgc aatcatccct caaggatcga cgggattagc
    25621 aagtcaggag gtcttatgaa tgagttcaag aagtgtatgc gcgtgtttag tcattctccc
    25681 tttaaagtac ggttaatgct gctctctatg ttgtgcgata tggtcaacaa caaaccgcag
    25741 caagataaac cttccgataa atagcggcgt cgcggtacgc cgcttcactc ctgcttttat
    25801 gcaggcataa cgcgttttgg tctgaaaaac cccacttttt gtcggatttg caatcccctt
    25861 cgcaaaagat ttgttcgtca gtagttgacc tgaacggcgg ctcgctctat cttcttgcag
    25921 ccctgcgtat attgcggctc gcggatgcgg acccctttcc cctcttcacg cactcttgca
    25981 ggtattgacc cttgacgcca gggtaagcac atggcgtttg ttacgatagt ggcatatgaa
    26041 tttaaccctc aaagaatcgc ttgttacccg tagccgggta tttagcccgt ggactgcgtt
    26101 ctacttttta cagtcgctat taattaacct cggcttaggt taccccttca gtttgctcta
    26161 caccgctgcg tttacggcta ttttgctttt gctatggcga acattgcctc gcgtacaaaa
    26221 agttctggtc ggtgtcagtt cgctggtggc ggcttgttat ttcccttttg ctcaggccta
    26281 cggcgcgcct aatttcaata cattgctggc attgcactcc accaatatgg aagagtcgac
    26341 cgaaatcctg acgatttttc cgtggtacag ctacctggtc ggcttattta tttttgcgct
    26401 cggcgtaata gcaatcaggc gaaaaaaaga gaatgaaaaa gcgcgctgga ataccttcga
    26461 cagcctgtgt ctggtattca gtgtggcgac attttttgtt gctcccgtgc aaaacctggc
    26521 ctggggtggc gtatttaaac tgaaagatac tggctatccg gtatttcgtt ttgctaagga
    26581 tgtcatcgtc aataataacg aggtgattga agagcaagaa cggatggcaa aactttccgg
    26641 aatgaaagat acctggacgg tcactgccgt taagccgaag tatcagacct atgtggtggt
    26701 gatcggcgaa agcgcgcgtc gcgatgccct cggtgccttt ggcggtcact gggacaatac
    26761 cccgtttgcc agcagcgtta acggtttgat atttgctgac tacattgccg ccagtggctc
    26821 cacgcagaaa tcacttggct taacgctcaa tcgcgtagtc gatggcaaac cacagtttca
    26881 ggataacttt gtcaccctgg caaatcgcgc gggcttccag acctggtggt tttccaacca
    26941 gggccaaatc ggcgaatacg atactgctat cgccagcatc gccaaacgtg cagatgaagt
    27001 gtacttcctg aaagaaggta attttgaagc agataaaaac acgaaagacg aagcgttact
    27061 ggatatgacc gctcaagtgc tggcgcaaga gcactcgcaa ccgcagctga ttgttctgca
    27121 tctgatgggc tcgcatccgc aggcctgcga caggacacaa ggcaaatacg aaacctttgt
    27181 gcaatcgaaa gaaacgtcgt gctatctcta taccatgacg caaacggacg atttactgcg
    27241 caagctgtac gatcagttac gcaacagcgg cagcagcttc tctctggttt acttttctga
    27301 ccacggtctg gcctttaaag agcgcggcaa agacgtgcaa taccttgccc atgatgataa
    27361 atatcagcaa aatttccagg tgccttttat ggtcatttcc agcgacgata aagcgcatcg
    27421 ggtgattaaa gcccgccgct cagccaatga cttcttaggc tttttctcgc agtggacggg
    27481 aattaaagcg aaggaaataa atatcaaata cccgtttata tctgagaaga aagccgggcc
    27541 gatatacatc accaacttcc agttacagaa ggtggattac aaccatctcg gaaccgatat
    27601 tttcgacccg aaaccttaaa acaaaaaatc cgccccgaga ggcggatttt ttatatcacc
    27661 aaagtgatta gaagcggtaa ccaacaccgg caatccaggt gcctacgtca acgctacgaa
    27721 tacggctctg ctcgtaagag aagtccagag caacgttttc catcgggttg aactgcagac
    27781 cagcaccgta ggagaaaccg tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg
    27841 tctggaattt accataaccc acccccacta caccgtagat gcttgcccag tcgttaatgc
    27901 ggtaagccgg accagcagtg atgccgtagt actggttttt gttgtagtca ccagagcttg
    27961 cagtacggct tttctcggtg taagtgaaag aaccgatcac acccagcggg ctgttgtctt
    28021 cttcatagcg gtatttcagg ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc
    28081 tctgtgcgta accgccagtt acggtagaag tcgcagctac ggaagtacct gcggtgaaag
    28141 ccagaactgc ggccagtgct gaaagacatg caattttttt cataaccacc tcaaatgtga
    28201 ttcaaataag tcctaagttt taaatatatc aaaaattaat gggaaactct tcgcgatttg
    28261 tgatgtctaa cgggccattt catgtaacag aacgtttcca tacaccgcta tccatctaaa
    28321 tttaaatcac tttttcagag aactgcgtaa gtattacgca tgttttccct gtcattcatc
    28381 cagattattc ctaatcacca gactaatgat tccatcaatc ctggcgcatt ttagtcaaaa
    28441 cgggggaaaa ttttttcaac aaatgctcaa ccagcattgg gtatatccag tacactccac
    28501 gctttactta agtctagata tttgtgggag aaaggatgcc tggttcatta cgtaaaatgc
    28561 cggtctggtt accaatagtc atattgctcg ttgccatggc gtctattcag ggtggagcct
    28621 cgttagctaa gtcacttttt cctctggtgg gcgcaccggg tgtcactgcg ctgcgtctgg
    28681 cattaggaac gctgatcctc atcgcgttct ttaagccatg gcgactgcgc tttgccaaag
    28741 agcaacggtt accgctgttg ttttacggcg tttcgctggg tgggatgaat tatctttttt
    28801 atctttctat tcagacagta ccgctgggta ttgcggtggc gctggagttc accggaccac
    28861 tggcggtggc gctgttctct tctcgtcgcc cggtagattt cgtctgggtt gtgctggcgg
    28921 ttcttggtct gtggttcctg ctaccgctgg ggcaagacgt ttcccatgtc gatttaaccg
    28981 gctgtgcgct ggcactgggg gccggggctt gttgggctat ttacatttta agtgggcaac
    29041 gcgcaggagc ggaacatggc cctgcgacgg tggcaattgg ttcgttgatt gcagcgttaa
    29101 ttttcgtgcc aattggagcg cttcaggctg gtgaagcact ctggcactgg tcggttattc
    29161 cattgggtct ggctgtcgct attctctcga ccgctctgcc ttattcgctg gaaatgattg
    29221 ccctcacccg tttgccaaca cggacatttg gtacgctgat gagcatggaa ccggcgctgg
    29281 ctgccgtttc cgggatgatt ttcctcggag aaacactgac acccatacag ctgctggcgc
    29341 tcggcgctat catcgccgct tcaatggggt ctacgctgac agtacgcaaa gagagcaaaa
    29401 taaaagaatt agacattaat taaatttaca tttctgcatg gttatgcata accatgcaga
    29461 atttctcgct acttttcctc tacaccgtct ttatatatcg aattatgcaa aagcatattt
    29521 attccgaaaa ttcctggcga gcagataaat aagaattgtt cttatcaata tatctaactc
    29581 attgaatctt tattagtttt gtttttcacg cttgttacca ctattagtgt gataggaaca
    29641 gccagaatag cggaacacat agccggtgct atacttagtc tcgttaatta ctgggacata
    29701 acatcaagag gatatgaaat tatgagtacc gctaaattag ttaaatcaaa agcgaccaat
    29761 ctgctttata cccgcaacga tgtctccgac agcgagaaaa aagcaacagt agagttgctg
    29821 aatcgccagg ttatccagtt tattgatctt tctttgatta ccaaacaagc gcactggaac
    29881 atgcgcggcg ctaacttcat tgccgtacat gaaatgctgg atggcttccg caccgcactg
    29941 atcgatcatc tggataccat ggcagaacgt gcagtgcagc tgggcggtgt agctctgggg
    30001 accactcaag ttatcaacag caaaaccccg ctgaaaagtt acccgctgga catccacaac
    30061 gttcaggatc acctgaaaga actggctgac cgttacgcaa tcgtcgctaa tgacgtacgc
    30121 aaagcgattg gcgaagcgaa agatgacgac accgcagata tcctgaccgc cgcgtctcgc
    30181 gacctggata aattcctgtg gtttatcgag tctaacatcg aataaatcca tcgctgatgg
    30241 tgcagaactt tagtacccga taaaagcggc ttcctgacag gaggccgttt tgttttgcag
    30301 cccacctcaa cgcacttatt tagtgcatcc atctgctatc tccagctgat taagtaaatt
    30361 ttttgtatcc acatcatcac acaatcgtta cataaagatt gttttttcat caggttttac
    30421 gctaaataat cactgtgttg agtgcacaat tttagcgcac cagattggtg ccccagaatg
    30481 gtgcatcttc agggtattgc cctataaatc gtgcatcacg tttttgccgc atctcgaaaa
    30541 atcaaggagt tgcaaaactg gcacgatttt ttcatatatg tgaatgtcac gcaggggatc
    30601 gtcccgtgga tagaaaaaag gaaatgctat gaagtctgta ttaaaagttt cactggctgc
    30661 actgaccctg gcttttgcgg tttcttctca tgccgcggat aaaaaattag ttgtcgcgac
    30721 ggataccgcc ttcgttccgt ttgaatttaa acagggcgat aaatatgtgg gctttgacgt
    30781 tgatctgtgg gctgccatcg ctaaagagct gaagctggat tacgaactga agccgatgga
    30841 tttcagtggg atcattccgg cactgcaaac caaaaacgtc gatctggcgc tggcgggcat
    30901 taccatcacc gacgagcgta aaaaagcgat cgatttctct gacggctact acaaaagcgg
    30961 cctgttagtg atggtgaaag ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg
    31021 gaaagtggtt gctgttaaga gcggtactgg ctccgttgat tacgcgaaag caaacatcaa
    31081 aactaaagat ctgcgtcagt tcccgaacat cgataacgcc tatatggaac tgggcaccaa
    31141 ccgcgcagac gccgttctgc acgatacgcc aaacattctg tacttcatca aaaccgccgg
    31201 taacggtcag ttcaaagcgg taggtgactc tctggaagcg cagcaatacg gtattgcgtt
    31261 cccgaaaggt agcgacgagc tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga
    31321 gaacggaact tacaacgaaa tctacaaaaa atggttcggt actgaaccga aataataacg
    31381 ctacacctgt aaagcgcact ggcagttccc tctcccctct ggggagagga ttagggtgag
    31441 gggcgcaaac ccgctccggg gccattaatt accctgaatt tgattattta caccacggta
    31501 acaggaacaa catatgcagt ttgactggag tgccatctgg cctgccattc cgcttctgat
    31561 tgaaggtgcc aaaatgaccc tgtggatttc ggtcctcggt ctggctggcg gtctggtaat
    31621 cggattgctg gcaggttttg cacgcacctt cggtggttgg atagccaacc acgtcgcgct
    31681 ggtctttatt gaggtgatcc gcggcacacc tatcgtcgtc caggtgatgt ttatttattt
    31741 cgccctgccg atggcgttta acgacttacg catcgaccca tttactgcgg cggtggtcac
    31801 catcatgatc aactccggcg cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat
    31861 tcacaaaggt tttcgtgaag caggactggc gctcggtctt tcacgttggg aaaccattcg
    31921 ctacgtcatt ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta accagtggat
    31981 catcagcatt aaagacacct cgctgtttat tgtgatcggc gtggcggaac tgacccgtca
    32041 ggggcaagaa attattgccg gtaacttccg cgcccttgag atctggagcg ccgtggcggt
    32101 gttctatctg attattaccc tggtgctaag ctttattttg cgtcgtctgg aaagaaggat
    32161 gaaaatcctg tgattgaatt taaaaacgtc tccaagcact ttggcccaac ccaggtgctt
    32221 cacaatatcg atttgaacat tgcccagggc gaagtcgtgg tgattatcgg gccgtccggt
    32281 tccggtaaat cgaccctgct gcgctgcatc aacaaactgg aagaaatcac ctccggcgat
    32341 ctgattgtcg atggcctgaa ggttaacgat ccgaaagttg acgagcgcct gattcgccag
    32401 gaagcaggta tggtgttcca gcagttttac ctcttcccgc atctgacggc gctggaaaac
    32461 gtcatgtttg gtccgctacg cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg
    32521 cgtgagctgc tggcgaaagt cggtctggca gaacgtgcac atcactaccc ttccgaactt
    32581 tctggtggtc aacagcagcg tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg
    32641 atgctgtttg atgaaccgac ttccgctctt gacccggaac tgcgccatga agtgctgaag
    32701 gttatgcagg atctggctga agaagggatg acgatggtga tcgtgaccca cgaaatcggt
    32761 tttgccgaga aagtagcttc gcggctgatc tttatcgaca aaggccggat tgcggaagat
    32821 ggcgatccgc aggtattgat caagaacccg ccgagccagc gcttgcagga atttttgcag
    32881 cacgtctctt aacttctctg cctccctttc acccgaaagg gaggcacacc agattcctct
    32941 catttaaaat cgcccctccc ccagcatcta tacttatctt tttgctctgt tttctcactg
    33001 gaggagtcat gcggtggatc ctgttcatcc tcttctgcct gctgggcgca cctgcccacg
    33061 cggtatccat acccggcgtt acaaccacaa cgacaacgga ctcaacgact gaaccggccc
    33121 cggaaccgga tatcgaacaa aaaaaagcgg cctatggcgc actggcggat gtgctggata
    33181 acgacacctc gcgtaaagaa ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg
    33241 cggaaccggt cccgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa
    33301 aggtcaccga agtcagccgc cattatggtg aagccctttc cgcccgcttc gggcaacttt
    33361 atcgcaatat caccggctcc ccgcataagc catttaatcc acaaaccttc agcaatgcgc
    33421 tgagccattt ttcaatgtta gcggtattag tgtttggttt ttactggctg attcgcctgt
    33481 gcgcactgcc gctgtatcgc aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca
    33541 gcaactggtt gcagcttccg gcgatgatta tcggggcgtt tattatcgac ctgctgttac
    33601 tggccctgac attatttgtc ggccaggtat taagcgacaa cctgaatgca ggcagtcgca
    33661 ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag
    33721 ccgtactacg cctgattttt tgcccaaacg tggcggagct gcgcccgttc acgattcagg
    33781 acgagagcgc ccgttactgg agtcgtcgcc tgagctggtt aagcagtttg ataggttatg
    33841 gcctgattgt ggccgtgccg attatctcta atcaggtgaa tgtacagata ggtgcgctgg
    33901 cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt gtacctgatc tttcgtaata
    33961 aaaaagagat tacccagcat ttgctcaact ttgcggagca ttcgctggcc tttttcagcc
    34021 tgtttatccg cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcgtgc
    34081 tgtttttctt ttcgttgttc gatccgggca acagcctgaa atttatgatg ggtgcaacgg
    34141 tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc
    34201 tggcaaaaac catcactctc tcgccacata ctcagcgtaa ctatccagag ttgcaaaaac
    34261 ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg
    34321 cggtaatgtt gctgttgagc gcatggggat tgttcgattt ctggaactgg ctgcaaaacg
    34381 gcgcggggca gaaaaccgta gatatcctga tccgtatcgc acttattctt tttttctcgg
    34441 cggttggctg gacggtgctc gccagtttga tcgaaaaccg gctggcttcg gatattcatg
    34501 gccgcccgct acccagcgcc cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg
    34561 cggtgattat cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg
    34621 cgccattgct ggcaggtgcc ggggcattag gtctggctat ctcgtttggt tcgcaaacgc
    34681 tggtgaaaga tatcattacc ggggtgttta ttcagtttga aaacggcatg aacactggag
    34741 atttggtgac tatcgggccg ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg
    34801 gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg gtcttcgata accacctttg
    34861 ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta cgatgttgat cgccatgaag
    34921 atgctgataa agccaatcag gcactgaaag atgcggtagc ggaattaatg gaaaacgaag
    34981 aaattcgcgg gctgattatt ggtgaaccga attttgccgg gattgtcggc ttaagcaata
    35041 ccgcgtttac actgcgtgtt tcgttcacca cgctgccact caaacagtgg acggtacgct
    35101 ttgccctcga cagccaggtg aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc
    35161 agacttatca ggtgctgcct actccgggcg cgaccccggc tgaaccgtta ccgccggggg
    35221 agccaacgct ttaacgctgg cgattgacaa aacggcggcg ctgctcgtcg ttcataaagg
    35281 tccaggcaat aaagcgactc tgcttttgcc cctgggccat ctcttttttt accaccttca
    35341 ccgcgcccac gtcggtcaga gcacgataca acggcggtaa gttttcacca cgagatacca
    35401 gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct ctcttcaatc atctttttga
    35461 taaaggcgac ttcaccacct tcacaccaca actcctgttg ctgaccacca aagttcagtg
    35521 catcgtcttt gttcagcccc aggttacggc gtttacgctc actacctgcc cgcgctgcag
    35581 cggcggaatc gtggaatggc gggttacaca aggtcgcgtc gtattgctcg tttttatgga
    35641 tgatgccgtt aaaaattgcc ccactctctt tttgccgacg cagacgaatg gcgcggttaa
    35701 gccccggatt agcactgata atcgcctgcg cactgcttaa cgcctggctg ctggtttcac
    35761 taccggtaaa acgccagcca tattcatgta cgccaatcag cggataaata cagttcgcac
    35821 caacgccgat atccagaatg ctggcattcg ctggaattgt tccgcttgct tcagccagta
    35881 aatcggcaag gtgatgaata taatccgccc ggcccggtac tggtgggcag agaaaaccgt
    35941 cggggatatc ccagttcgct acggcgtaaa aatgggccag caacgcctta ttgagcgcct
    36001 tcaccgccag cggattggca aagtctacgc tttgctcccc ggcgggtgta agcgtgagga
    36061 attgcctgag ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac
    36121 ggttgcgcgg atgcaacccc ggtttctggg cggacatagc attctccttt aagcagcggc
    36181 gtaagatacc cgcataacgc gacagggtaa atatctggct gcgtttatca ttccccaaaa
    36241 ccttaacatg gctatggttt atggcaattc atcataaaaa tcagcattat gcatgtcatt
    36301 aatttgtctt attttgtaaa aatatatcgt tcaaaaaata agcacatctc tgtcaatttt
    36361 ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag gcactacccg ttgtatttat
    36421 gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg ccaccgtcct gagcggaatc
    36481 tctctcacgg cttatgccgc gcaaccgatg agtaacctgg acagcggtca gctacggccc
    36541 gccggtaccg tttcggcaac tggcgcatca aacctaagcg atctggagga taaactggca
    36601 gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta attccgcagg cggaaatgac
    36661 cagatgttcg gtactgcaac catctacaaa taaccccacc aacgtaactg cattcgcctc
    36721 tggatgcagt tttcgcctgt tttgtaatca aaaaacatca aatatgaact caatgtaaat
    36781 aaatgtattt ctttttcgcg caatgggtga tagaaaatcg cgccaagtga taatgcttat
    36841 caaaattatt atcacttgca cgaacactat cacgggatta acagtggcat cgcatccgca
    36901 gagatgcttt ctcgtggcag tgaaaatttc aacatataag aaaaagtcac ctgcaaaatg
    36961 gaaaacaatc gcaatttccc tgccagacaa tttcattcgc tcacgttctt tgccggtctt
    37021 tgtattggca tcacgcctgt ggctcaggca ctcgccgccg aagggcaaac taacgcggat
    37081 gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca atctgccgat
    37141 ccgaaattct cgcgtccggt agcggatact acccgcacga tgacggtgat ttctgaacaa
    37201 gtgattaaag atcagggcgc aaccaacctt accgacgcgc tcaaaaacgc ccccggcg
//
LOCUS       DAQSWQ010000035        36659 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.035, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000035 DAQSWQ010000000
VERSION     DAQSWQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.035"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..460)
                     /locus_tag="R8G18_001787"
     CDS             complement(<1..460)
                     /locus_tag="R8G18_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEE9795506.1"
                     /translation="MRFCLILITALFLAGCSHHKAPLPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
     gene            complement(538..1287)
                     /gene="btuD"
                     /locus_tag="R8G18_001788"
     CDS             complement(538..1287)
                     /gene="btuD"
                     /locus_tag="R8G18_001788"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HEE9795507.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(1287..1838)
                     /gene="btuE"
                     /locus_tag="R8G18_001789"
     CDS             complement(1287..1838)
                     /gene="btuE"
                     /locus_tag="R8G18_001789"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HEE9795508.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(1901..2881)
                     /gene="btuC"
                     /locus_tag="R8G18_001790"
     CDS             complement(1901..2881)
                     /gene="btuC"
                     /locus_tag="R8G18_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HEE9795509.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(3074..3511)
                     /locus_tag="R8G18_001791"
     CDS             complement(3074..3511)
                     /locus_tag="R8G18_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HEE9795510.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLECDIANLKGELKSDTANLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            complement(3612..4112)
                     /locus_tag="R8G18_001792"
     CDS             complement(3612..4112)
                     /locus_tag="R8G18_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795511.1"
                     /translation="MEIKKPTKKELYDYLLSKYIEDKCKEEADEINKKSMSRVKKHKE
                     RLMEITPEIFFRFLSEKGVSSVCPSCGSSRLSVPESMDLCWDKNKKPENFNNLPLEEQ
                     SELIKENIKHYVSYAFLGDVKSIPDMRKTYYTLHCLNCGYLSLYRTSAVLKWLEKDKA
                     QDGDNG"
     gene            complement(4115..5053)
                     /locus_tag="R8G18_001793"
     CDS             complement(4115..5053)
                     /locus_tag="R8G18_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEE9795512.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGM"
     gene            complement(5142..5453)
                     /locus_tag="R8G18_001794"
     CDS             complement(5142..5453)
                     /locus_tag="R8G18_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9795513.1"
                     /translation="MSIEISKKLKAIRESEGLSQAKFADSIGIAVGTVKQYETGIRGV
                     GTEVLLKITMHPEFKKYTTWLMSNETNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            5549..5827
                     /locus_tag="R8G18_001795"
     CDS             5549..5827
                     /locus_tag="R8G18_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEE9795514.1"
                     /translation="MAKVLNTYEQADFERLAAFYPYRNEHGLPVLEESLEDYAKRTNQ
                     SILAVRRQADRSVIPVTQEGRNTKRKVNLFAIFLKTIRNAEKYVQMTK"
     gene            5842..6180
                     /locus_tag="R8G18_001796"
     CDS             5842..6180
                     /locus_tag="R8G18_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HEE9795515.1"
                     /translation="MLKQRRNFCTGTERHANRFTTSASRSNIRYSLSDTHATPDGYPV
                     KQIGEHAWLIEKAGIVIHKCPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVDRLLR
                     GQSFIKQADL"
     gene            6191..6478
                     /locus_tag="R8G18_001797"
     CDS             6191..6478
                     /locus_tag="R8G18_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795516.1"
                     /translation="MTKDNAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEDEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            6490..6732
                     /locus_tag="R8G18_001798"
     CDS             6490..6732
                     /locus_tag="R8G18_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HEE9795517.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            6729..6842
                     /locus_tag="R8G18_001799"
     CDS             6729..6842
                     /locus_tag="R8G18_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795518.1"
                     /translation="MSIFISWLVLIISVVCAIGIMRIINSVKKIERFFTEE"
     gene            6929..7132
                     /locus_tag="R8G18_001800"
     CDS             6929..7132
                     /locus_tag="R8G18_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795519.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVQVNLIRQVQESRR"
     gene            7129..7374
                     /locus_tag="R8G18_001801"
     CDS             7129..7374
                     /locus_tag="R8G18_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEE9795520.1"
                     /translation="MTILDYIAANPGCSSGEIAAALNTPTTTINAELRRLWRSGLVTR
                     KERKTGGRFSYQVNLMPFGCSNPLTQMFNQLLREIRA"
     gene            7516..7881
                     /locus_tag="R8G18_001802"
     CDS             7516..7881
                     /locus_tag="R8G18_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795521.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            7888..10710
                     /locus_tag="R8G18_001803"
     CDS             7888..10710
                     /locus_tag="R8G18_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HEE9795522.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAHRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDGHWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRHEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFATVRKKLNRDGLRWYGIRTAEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLTQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            10787..11746
                     /locus_tag="R8G18_001804"
     CDS             10787..11746
                     /locus_tag="R8G18_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HEE9795523.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            11751..12062
                     /locus_tag="R8G18_001805"
     CDS             11751..12062
                     /locus_tag="R8G18_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HEE9795524.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLGSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPVPQPVPTAQITSQIAGNIRALFP
                     E"
     gene            12123..12647
                     /locus_tag="R8G18_001806"
     CDS             12123..12647
                     /locus_tag="R8G18_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795525.1"
                     /translation="MKKYEYMLMKEALRAGLAPCPVSVEAVVGNTIFRTLRNRVSVKM
                     VHQPPKSTSMIYLPLPEAIKLMRSLKFAIWMQTQINKAVQNTFVKRLIPELAARCAKC
                     TRPALQFDLSLIQQATEAELYRLSPIRDAHTEASEMFRLMFQFNFAVRRTRVQVNRMM
                     QEQEELTQYKGGSQ"
     gene            12644..12883
                     /locus_tag="R8G18_001807"
     CDS             12644..12883
                     /locus_tag="R8G18_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795526.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMILRAEKSK"
     gene            complement(13575..14621)
                     /locus_tag="R8G18_001808"
     CDS             complement(13575..14621)
                     /locus_tag="R8G18_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEE9795527.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(14621..16372)
                     /locus_tag="R8G18_001809"
     CDS             complement(14621..16372)
                     /locus_tag="R8G18_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEE9795528.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAEMHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTCFS
                     TPSTETHEAYAYWNGDRWNEKKAAHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDATGGHGDAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            16527..17363
                     /locus_tag="R8G18_001810"
     CDS             16527..17363
                     /locus_tag="R8G18_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HEE9795529.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVESVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            17387..18439
                     /locus_tag="R8G18_001811"
     CDS             17387..18439
                     /locus_tag="R8G18_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HEE9795530.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKDSTELTKKINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWSAHSDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANPDALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            18485..19285
                     /locus_tag="R8G18_001812"
     CDS             18485..19285
                     /locus_tag="R8G18_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEE9795531.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKADIKKALLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYAVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSVFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNQNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSESKKTTRKPATRKTTATQKAKRG"
     gene            19387..19881
                     /locus_tag="R8G18_001813"
     CDS             19387..19881
                     /locus_tag="R8G18_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HEE9795532.1"
                     /translation="MSMVARTEPGPAEDDITDTEDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            19881..20078
                     /locus_tag="R8G18_001814"
     CDS             19881..20078
                     /locus_tag="R8G18_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HEE9795533.1"
                     /translation="MKVKTLEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCRQVFLEA
                     GQEVEMPEPEKKKQREMIQLW"
     gene            20072..20374
                     /locus_tag="R8G18_001815"
     CDS             20072..20374
                     /locus_tag="R8G18_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9795534.1"
                     /translation="MVTGFFQRVSDWITFWASVTATTIGVMTISEKIALAGLLLGSLS
                     ALHAWRHRRLMETAMQRRNELIASILAQSDARPLHDSERQALNILRDNDTHDKAAR"
     gene            20355..20903
                     /locus_tag="R8G18_001816"
     CDS             20355..20903
                     /locus_tag="R8G18_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEE9795535.1"
                     /translation="MIKLPVKRYAVAAIVALGFSIAPHKLRTSPEAQIRIATWEDCRA
                     TPYRDIAGVMTVGCGSTGHVENRLYTETEVAGRWVNDMQHAENCINQNFSGNAMPQSA
                     FEAMTDAAFNLGCRNLMWFRDKNRNYHRTTIWKHAQAHNWPDMCNRLTDFVNSGGERS
                     QGLVNRRTDFRAWCLRDTGGQS"
     gene            20900..21373
                     /locus_tag="R8G18_001817"
     CDS             20900..21373
                     /locus_tag="R8G18_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795536.1"
                     /translation="MKVRYILMMALTVLTLLTITLWQTFRLGQEDGHNRYTQAQLDDY
                     SRTLKEAGTIISTASGVLRDVMTARQNRQQEGEIRREQLRNDITTDACARAVPDARFT
                     ERLHQHAERVRAAAVVPFHPGRADRPRTSPAVSGDAIMGKHRDMERQPHGRTGNL"
     gene            21445..21912
                     /locus_tag="R8G18_001818"
     CDS             21445..21912
                     /locus_tag="R8G18_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9795537.1"
                     /translation="MLKTDSLREAMTRSCRWCHANPEKFTIFVESGNIETTGETPSFV
                     YRYQIVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            21905..22540
                     /locus_tag="R8G18_001819"
     CDS             21905..22540
                     /locus_tag="R8G18_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEE9795538.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTRRVSMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            22537..23118
                     /locus_tag="R8G18_001820"
     CDS             22537..23118
                     /locus_tag="R8G18_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEE9795539.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNT
                     TRAGDFNVWFPPSPGEQVAIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASISVTLNTPVVECTDLLRTATLDVTKGGTMSGNI
                     THSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            23136..23465
                     /locus_tag="R8G18_001821"
     CDS             23136..23465
                     /locus_tag="R8G18_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEE9795540.1"
                     /translation="MNPDGTGNLNDMAHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSATVIALTRWEPRIALNAIDVVWKAGGHAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            23469..24365
                     /locus_tag="R8G18_001822"
     CDS             23469..24365
                     /locus_tag="R8G18_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEE9795541.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREVVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEAARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDVVMENDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            24358..24888
                     /locus_tag="R8G18_001823"
     CDS             24358..24888
                     /locus_tag="R8G18_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HEE9795542.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPADLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFNGEPGTFKIDVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            24891..26861
                     /locus_tag="R8G18_001824"
     CDS             24891..26861
                     /locus_tag="R8G18_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9795543.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQATAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVTLDATHQNHIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVNYVDDKL
                     KEHEQSRRHPDASLTAKGLVQLSNATNSTSETLAATPKAVKAVMDETNKKAPLNSPAL
                     TGTPTTPTARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATT
                     MTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAV
                     IEYLGLQETVNQASGALQKNQNGADIPGKDTFTKNIGACRAYSSWLNIGGDSQVWTTA
                     QFISWLESQGAFNHPYWMCKGSWAYANNKVITDTGCGNICLAGAVVEVIGTRGAMTIR
                     VTTPSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGNDKAV
                     GWNWNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNGGIFYRSARDGYGFEAD
                     WSEFYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLLSVQTWNGPGWSDRSGYVVTG
                     SVNSNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            26864..27397
                     /locus_tag="R8G18_001825"
     CDS             26864..27397
                     /locus_tag="R8G18_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEE9795544.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGVVI
                     KRTYTGEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(27426..27953)
                     /locus_tag="R8G18_001826"
     CDS             complement(27426..27953)
                     /locus_tag="R8G18_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEE9795545.1"
                     /translation="MMHLKNIKAGNAKTVEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(27955..28941)
                     /locus_tag="R8G18_001827"
     CDS             complement(27955..28941)
                     /locus_tag="R8G18_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9795546.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNNSDADTDSY
                     MWFETGNNGNEYFKWRHRIIGTRPKDLMNLKWNALSVLVEALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLDVTGDIF
                     ANRFAINSSTGMWIHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGVNN
                     WQTWNFQCPSGYVLTGINVQDTGKNSADNIAGVHYRPVQKYINGTWYNVASI"
     gene            29046..29633
                     /locus_tag="R8G18_001828"
     CDS             29046..29633
                     /locus_tag="R8G18_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEE9795547.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGISTIYRYHPAGESIGTIEKSQETK"
     gene            complement(29669..30157)
                     /locus_tag="R8G18_001829"
     CDS             complement(29669..30157)
                     /locus_tag="R8G18_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9795548.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDERIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(30170..32977)
                     /locus_tag="R8G18_001830"
     CDS             complement(30170..32977)
                     /locus_tag="R8G18_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEE9795549.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTGQYNNQLERERRALARVT
                     QARERYSRAQETAGKLKTGGALATGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLAKMGIRGSDAGTALRKSLANLSSPPKAAAEALKELGVSVADA
                     KGKMRPMENVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMGLVDAAGDGFLAKLR
                     KELEGARGEAERTAKVMANNLDGNLKSLGSAWEGLRIRISDLVDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLATTATVGSLSLVIGVLHGKLATLRLGFSLLTES
                     MNVVRVLPALWGMVTGSVSLLGGAIGALFSPVSLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDVVRDGIIQVFNWFKSLLSPMESSKETLDKCASA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(32964..33119)
                     /locus_tag="R8G18_001831"
     CDS             complement(32964..33119)
                     /locus_tag="R8G18_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HEE9795550.1"
                     /translation="MCFEHIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(33128..33493)
                     /locus_tag="R8G18_001832"
     CDS             complement(33128..33493)
                     /locus_tag="R8G18_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9795551.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGEKNEQATEA"
     gene            complement(33548..34060)
                     /locus_tag="R8G18_001833"
     CDS             complement(33548..34060)
                     /locus_tag="R8G18_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HEE9795552.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGRDIVP
                     DRIRSALGLG"
     gene            complement(34060..35244)
                     /locus_tag="R8G18_001834"
     CDS             complement(34060..35244)
                     /locus_tag="R8G18_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HEE9795553.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLPRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVIGAIRAKLDSLVTAG
                     KLIGASCWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            35402..36511
                     /locus_tag="R8G18_001835"
     CDS             35402..36511
                     /locus_tag="R8G18_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HEE9795554.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRAEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
BASE COUNT         9224 a        10003 c         9343 g         8089 t
ORIGIN      
        1 ttcgtctcgc ctgccagaaa tttttctgcc aatagacatt atcaagtgag gaacgcatca
       61 ctcccttgct ggtagaggcg tggataaatt ggttgttggt atcgtaaata cctacatgca
      121 aaccattttg tccggaaccc gttttgaaaa agaccaggtc accaggcagc aactcgtctt
      181 tatcaatttg cgtgccgata gaggcttgtt ctttggtttc tcggggcagc tgcaaatcga
      241 aacgatcgcg catcgttaca accacaaatc ccgaacagtc cacaccgcgc cgcgtcatgc
      301 caccataacg atacggcgtg ccatgccagc tttgtagctg gtcgttcaaa ccggcaataa
      361 cggtaatcga atcagaaagt ctggcatttg gcagcggtgc tttatggtgg ctacacccgg
      421 ccagaaacag tgctgtgatc aaaataaggc agaaacgcat tccgtacggt tcctctgttt
      481 tttattcttg cattaattta gcgtcgtaat tacccgattt tcaagatact aatgaaatca
      541 gatggtcgaa atcagcattc tgtgaccttc gatatccaga cggcgaaaat tcatcccata
      601 ggcctgcgcc agatttggcg gcgtgagcac ctcttctctg cgtccactgg ccagcatttt
      661 tccacctttt agcaaccacg cccgatgcgc atgacgcaat gtgtggttga gatcgtgact
      721 gctcatcaca atcgccagtc cttgctgaca cagagcgctc agaattttgt ctaacgcact
      781 ttgttgcgca acatcaagac tgttcatcgg ctcatcaaga agcagcaatt ggcctgcggg
      841 attggcttgt ggtgtgattt gcagcaccac cgcagcaaga cgtacgcgtt gccattcacc
      901 gccggaaagt tgattggtgc tacgtccgag tttgtcatca agagccagcg cccctgcgac
      961 atcattcagt agttcggtac gcgttttatc gtgctgatgc agtgtcagat agtgccagac
     1021 cggcattgca aacggcggcg tctgctgttg tgaaagatag gcgcgatgca gcgcgagttt
     1081 tgttgcggac catgcttcca gtggttgccc cgcgaactga atacttccct taccgctggt
     1141 cattccggcc attcgtgcca gtaaggtact cttacccgcg ccattcggcc ccaccaggtg
     1201 caggatctcc ccagcccgaa cctcgccaga aagtggcccc aggcgggtag attccgcaac
     1261 atcctgtaac tgcatcacaa tagacattat tttgccaacg ccagtttaat gctttccatc
     1321 acaatggggt cttccggcgt catatccggg gaaaaacgct ggatgaccaa tccgtccctg
     1381 ccaactaaaa atttctcaaa attccataaa atatcatcag ggtacagcgg tgcacgacct
     1441 ttgctgacca tacgggcata gaatccgctc tcttccggcg cgactgcggt cggcgctgcg
     1501 gcaatcaatt tttgatacag cggatggcgt ccttcgccgt taacttcaat cttactgaac
     1561 atcgggaacg tcacccccca tgtggtggta cagtaagttt taatctcttc atcgctgccc
     1621 ggttcttgtt ccagaaactg gttgcacggg aatcccagca ccacaaaacc tcgatcggcc
     1681 caggctttct gaatattctc caactgctca tattgcggcg ttaagccaca ctttgaggcg
     1741 acattgacaa tcaacagcac attaccggcg tacttctcca gcgtggtcac ttcaccgtcg
     1801 atatctttca ctacggtcgt cagaatggaa tcttgcatcg tttctcctgg gtgtggtcag
     1861 taaaaatctt agcttttaat catagaccgt ctttttgcgg ctaacgtcct gcttttaaca
     1921 ataaccagat aaacaccggc gcacccaacg ttgcggtgac cacgccaata ggcagctctg
     1981 cggcagctaa tgccaggcgc gctacaatat cggccagcag cactgcgctc gcccctgcca
     2041 gcgcgcagcc gggaagtaat acgcgatgat cggttaaacc acacaaccga agaatatgtg
     2101 ggatcaccag accaataaag ccgatagcac ccgccagcgc cacactgacg ccaaccatcc
     2161 agccggtcgc tgccaccagc acattgcgcc agaaccacag gggtaaaccc agttgccgcg
     2221 ccgagatctc gccaagtgct aacatattca tcggcctgga ctgacaacag atccacaaca
     2281 acacggggat caatgccagc atcagccagc tttgccgcca gtctacgccg ccaaaaccgc
     2341 ccatcatcca gtacatcagc tgacgtaaat caacagaggt ggaaaagtag atagcccacg
     2401 tcattagtgc gctacagata atccctaatg caacgccagc cagcaataac cgactggtcg
     2461 aaagatgacg acgggcgaaa cgtaagagta ttaaagtgat gataagcgcg ccagcaatcg
     2521 cacacagccc tagcgcccag ttggggagtt gcccttgccc aagcaatacc gcggcgataa
     2581 gccccacgcc tgcgccgtta gagacgccaa gtagtccagg ttctgccaga ggattttcaa
     2641 acaacgcctg cattacagcg ccggatatag ccagcgccgc accaaccagc aatacagcca
     2701 gcgtacgtgg caggcgaatt tgccagacga acagttcgcc acgaggagta aaccagtcac
     2761 ctggcgaaat ccattgttca ccggcgcaaa ggcttaagag aagcgccagc agcatcaaaa
     2821 ctgacaggca taataaccag cgaatatttt gtcgctgttg ttggcgggca agtgtcagca
     2881 tggtatccgt tctgctgaag tgtcatggcg ttgattttac ggtgactctt cgacagtgaa
     2941 aagaaaaaag gccgcagagc ggccataaac acaaacaaaa atcaataagt taggtaatta
     3001 tcaaagactt acagacacac aaaaacacag ccaaccacaa caaataacag agatgtggtc
     3061 aatttgtgga tcatcagcca acaaatttag caacaaatac agcaaccgcc gctgtgaaaa
     3121 tggttgtgca cactggcaca ataatgccac tccaacgcca cttcgtatta tcatcaacct
     3181 tgtcaaaccg ccgattcatc tcatccataa tcttgtcaaa gcgtttatcc atcgcttcgg
     3241 taagctcacc cttcaggctg ttaatgtctg atttgagctg ctctttcagg ttagctgtat
     3301 cagatttaag ctcacctttc agattcgcaa tatcgcattc aagatcacct ttcagatgtg
     3361 ctatgtctgc tttgagttca cctttcacct caccaacatc ggctttagtt gagaggtttg
     3421 tagtgcgttc agcaagcgtc gcaaggtcgt ttcttgttag cgataaatca ccttcgagcc
     3481 gctcaattcg cctttcaagt ttatctgaca ttccgcctcc actactgccg ccatcgccac
     3541 catgataaag cacatcacgc tcagaatgcg acacactacc tgctgaatta ccgaaaagat
     3601 ttcttgctac attacccatt atcaccatcc tgcgctttgt ctttctccaa ccacttcagc
     3661 accgcagacg tacgataaag gctcaggtaa ccacaattca ggcaatgcag cgtgtaataa
     3721 gttttgcgca tatcaggtat gctcttaaca tcacccaaaa aagcataaga tacataatgc
     3781 tttatgttct ctttaattaa ctcactctgt tcctccaggg gtagattgtt aaaattttct
     3841 ggtttcttat ttttatccca gcaaagatcc atactttctg gaacagataa tcgcgacgat
     3901 ccacatgaag ggcagacact ggagaccccc ttctcagaca agaaacggaa gaagatttct
     3961 ggcgtaatct ccatcagccg ctctttatgt tttttgacac gactcatcga cttcttattg
     4021 atttcatcag cttcttcttt gcatttatct tctatatatt tcgatagtaa atagtcatat
     4081 aactcttttt ttgtaggttt ttttatttcc atagttacat tcctcccaag ggattaaacc
     4141 tcaccgcatc ctgcaagtaa tccggcgcaa gatgggcata aatcatcgtt gtctgaatct
     4201 ttgcgtgccc cagaattttc tggagcgtca gaatattgcc gccgttcatc atgaaatgac
     4261 tggcgaaggt gtggcgcagc gcatgaacag cctggccgtc aggaacatca ggtgcgaccg
     4321 ttttgatgac atcgcgaacc aatgaataat ccagcgtcgg aaacaccagt ttcccgcccc
     4381 gttttttgat cttttcaaac aggctttcag aaataggaac ggtacggttt ttgctgttct
     4441 tcgtttttga aaaagtgatt cgacaatgaa gaacacggcg ctgctccagt gccgctacct
     4501 cgccccatcg cgccccggtc gacagaagga tttcgacggc cagccgttca tcgggatttt
     4561 cagccagtgc atccagcaac tgaacacatt cagacttact cagatatccc atttcacgct
     4621 cgttaacctt cattccttta aggccttgaa cggggttatc gttaagaaaa tggccggatg
     4681 agatgagtgc ggtaaacatc gcgcttaacg ccccaatctc tcgatttatg gtgctgggct
     4741 gtatcccctg ctctatcctg gacacacgta gctcggtgag catcgttgta ttaagtttat
     4801 gcacgcacgg gtcatccatt gcctcactca agcgcagcaa tttaaggcgc gtgttatgcc
     4861 ctgacttcat tagctggccg tggtatttcc accacaagtc aataagcact gacagcggac
     4921 ggcgatcaat ggagtttcct ttccactcat tgttatgctg ttgcgccagc acccaccgct
     4981 catataaaac tgcatccgat ttcgttttaa attttttgcg aatgcgtttg cctttacgcc
     5041 cctcaggacg catgtcaaga agataccctc ccggaattga ttttatgctc attcgtgaag
     5101 ccccagcgtt acaagaccac catgccccca gcgttccatg attagccggg ctgtgtgcca
     5161 gtcttgcggg atttttgaga agacgatgtg ttttctggcc catcagggga gagagaagga
     5221 ctgatctgcc cagcagcctc atttgtttcg ttactcatca accacgtagt gtatttttta
     5281 aattccgggt gcattgtgat tttcagtaaa acctccgttc ccacacctcg aataccagtc
     5341 tcgtattgct taaccgtacc aaccgcaata cctattgaat ccgcgaactt tgcctgacta
     5401 agcccctcag attctcgaat tgcttttagc ttctttgata tctctattga catggtgtgc
     5461 acctgaatac tatattggtt atcagttgag taccacttga cgctacaaag agccacaaat
     5521 agcaccagat tgaataggtt atcacaccat ggcaaaagtc cttaacacat acgaacaagc
     5581 ggattttgag cgtttggcgg cgttctaccc ataccgtaat gaacatgggc taccggtact
     5641 tgaagaaagc ctagaggatt atgcgaaacg cacaaatcaa agcattctcg ctgtaagaag
     5701 gcaggctgat agatcggtaa ttcccgttac tcaagaagga agaaacacaa aacgcaaagt
     5761 aaatctcttc gcaattttcc tgaaaaccat cagaaacgca gagaaatacg tgcagatgac
     5821 aaaataacga ggtgtcattt tatgctgaag caacgccgta atttttgtac cggaacagaa
     5881 cgccacgcta accgtttcac taccagtgca tcacgcagca acatccgcta cagcctgagt
     5941 gatacacacg caacgccgga tggctaccca gtaaaacaaa tcggcgagca cgcctggctg
     6001 attgagaaag ctggaatcgt gatccacaaa tgcccacgca atccgtttac cggaaaccgc
     6061 atttttgcac tgagctgtgg cgacaatcag ttcgggcagg atttcacatt atacgaagca
     6121 cttcgcacgg ttgatcgtct gcttcgcggg caaagtttta ttaaacaggc tgatttataa
     6181 caggtgcttt atgaccaaag acaatgcaca aggtgtattt atccgtttta ttgattttcg
     6241 cggtgaactg ttattacgtg catccgctat tgacggagtg actccggcgg gtaaaaacgg
     6301 agccgacgaa gccacttacg tttatctgaa cggcacgcga ctgcttgtgg aacttccgta
     6361 ccagaccgta cgagaaatca tcagcgaaga tgaaaaggca cgccaggtta atggcgatga
     6421 accctatatc gaaattattt gtatggattc agaagctgaa atacagaaag cagattaaag
     6481 ggcgttgtga tgggcaaaga atataaaact ctcattaaca aagcacttga gcgtttttat
     6541 tttcgcttaa gtgcatcagg cgctcatgct gaacgtgcgg cccgtgactc attgaccaga
     6601 gcaatccgaa gtctgtatga cgtggctttt tacgctgatg atctggatgc acttaacgaa
     6661 ctttccgagc tgatctgtgc cgcagaatgc ggggaacata ttgaaccgta taagctggga
     6721 aatatcgcat gagtatattt atttcatggc ttgttctgat tatttcggtg gtctgcgcca
     6781 ttgggattat gcgaattatt aattcagtaa aaaagattga acgctttttc actgaagaat
     6841 aaccgcgcaa ataagacccc aggttaaata agaaaatgtg aaaacaatcc gcattcgcgg
     6901 aggtattcgc acacgccaag gaggcgtaat ggcaattaag cattttcctg tcgttcgttt
     6961 cacctccaga ggacgtgaat acgaagttga cgaacgcctg attaccacaa tcgacaaaca
     7021 ccgttcagaa aaggatgcac atcatatcta tctcactgac ggcacttact tctgcgccac
     7081 caacgtggtg caggtgaatc ttatcagaca ggtacaggag tcacgcagat gaccattctg
     7141 gactacatcg ctgccaatcc gggttgtagc agtggagaaa tcgccgcagc actgaataca
     7201 ccaaccacaa ccattaatgc ggagctacgc cgtctctggc gcagcggttt agtcacaaga
     7261 aaagagcgca aaacaggcgg tcgcttttct tatcaggtaa acctgatgcc gtttgggtgt
     7321 agcaacccac taacccagat gttcaaccag ctactgaggg aaatcagagc atgagcacct
     7381 ccaactgccg gaaaccacgt cgggcttcag cagctcatcc ggcagcaaaa caaactccat
     7441 taattcctgt tccgggcctt tcctgcacct tgcggcggga ggccttcgca catctgtaac
     7501 aagaggattg ccgcaatgat tctcgccaac gactttcttg aatacctgct caacacagag
     7561 cgtgatcttg ccgctcgcgt gcgtgatcgt tatgacatgt acctgaaatc cctgcctgta
     7621 ccgcagctcg ctgacggaaa gattgttatt gatggtcgct acatgattga cagccacgag
     7681 ggaaattaca ggctttaccg cattgaaggt ggcaccccgt ccgttattgg catttaccag
     7741 cgcccatcct ctgcaatcgt cgatgtgatt gccgacagca tccgcatcac acatcgccat
     7801 gccgacacag aagacaccgt gctggaaatt cagcggctgg ctacagtctg ccgcgacacc
     7861 ctgaatggca tgacgaagta aatcactatg acggcagagt acatcaggga ctggcaacaa
     7921 ccgcgccacg cagtggggcg tgaaggaacg gggatccccg ctcctgaatc cgcgctttcc
     7981 tcctggctgg atgcccaccg ggcagagaac gagcgccgcc aggaaatggc tgatgcggcg
     8041 ttctccgcca cgccactggg caacctgatt aataaaagcc tggacgcaca ggaaaaacag
     8101 gacaaaacca tcacactggc aggagacgcc agaaaacagg cacgcggcgc ggtggatgaa
     8161 gccatggcct cgctgcgcct gctgccgtcc tatctgcgcg atccgcttat tcgccacctc
     8221 tccttcctgc gcaaaaaaca ggaagccgat cgccggaaag gcaaaaagag ctggcaggcg
     8281 gaacgctatg cacgcggaac cctgcgcaaa atattcgaac gtctggatcg cactgacgga
     8341 cactggctga caccgggtta tcgctccctt gccggacgtg aacgcctgga cgatttgctt
     8401 tacctgccgc aactcaacaa acaccagata cagacgctgg ccaccatgac ggcggcgatg
     8461 ttcagcagca ccttcgaaaa actctgcgat ggttttggcg cgactgatgg cgagctgacc
     8521 atggatgtaa cgctgaaggc gtatcagatg ctggcccgca tggcgttaca cttacacgcc
     8581 atgcctccgc attatgacgc actgacaaca gacaaagacc ggaggcacga accggacaca
     8641 gaactgctgc cgggcgcaat ccttcgcctg acctgtgcgg aatggtggaa acgcaaactg
     8701 tggctgttac gttgcgagtg gagagaagaa caacttcgcg ccgcctgtct ggtttccaga
     8761 aaaacatcgc cctatctgag ccaggacgcg ttaagcgagt ttcgcgcaca gcgcgagaaa
     8821 acacgcgatt tcctgaaaag tttcatgctg gaaaatgaag acgggttcac gattgatctc
     8881 gagacggtgt attacgcggg agtaagtaac ccggttcacc gtaaggcaga aatgatggcc
     8941 accatgaagg ggctggaact tctggccgaa gcccgtggcg acaaagcggt gtttctgact
     9001 gtcacctgcc cgtcaaaata ccacgccaca acggagaacg gtcatccgaa tcccaaatgg
     9061 aacggggcca caatgcgcga ctccagcgat tacctggtta acacgttttt tgcgacggtc
     9121 cgcaaaaaac tgaaccgcga cggcctgcgc tggtatggca tccgcacggc ggagcctcac
     9181 catgacggca ccgtgcactg gcatatgatg gtctttgctc atccggaaga aatcgacacc
     9241 attgtgtccc acacccgcga tattgccatt caggaagatc gtcacgagct gggcgatgac
     9301 ataactccgc gctttaaggc ggagtatgta gacggctcaa aaggcacgcc aaccagctac
     9361 atcgccacct acatcgggaa aaacctggac agccgcgccg tggatggcat cgacccgaaa
     9421 acgggcaagc cacgcgttga ccacgaaacc ggaaaatcaa tggccgagag cgtggaacgc
     9481 gccatcggct gggcgcgcct tcaccgggtc cgccagttcc agttctttgg catcccctcc
     9541 cgccaggtat ggcgtgaact gcgccgcctt gccagccaga tggcacgcaa cccggaaggc
     9601 ccgcaacggc tgaaggatga cgcaatggat gcggttcttg ccgccgctga tgccggatgt
     9661 tttgccacct acatagagaa acagggcggc gtacttgttc cacgcaaaga ctacctgatt
     9721 cgcaccgcct acgacctcgc cgatgagctg aacgattacg gcgaacagag cgtacagatt
     9781 tacgggatct ggtcaccact catcggggaa tcctcccgtg tgtgcacgca tccggataac
     9841 tggaagctgg taagacgcaa accggaagcg gaagacagcg cccgcgaaaa tggttttgac
     9901 cttcagggcg gccctgccgc cccttggact cgtggcaata actgtccccg tgtacaggaa
     9961 acggacaaca acgggacaga acagccggaa gaacggccag caccgtggcc gcagctccct
    10021 gacggcgttg atgtggatga atggatgcgc tcactgaaac ggcacgaacg ccgggcgctg
    10081 atgcgttcgc ttcgtgacaa acaggcaaaa aacagcagtg atgaaatgca gagctggaca
    10141 cagagccgca aacagcagcg gcctttgcct gataaccacg aattactcgc taaagaatgg
    10201 cgggagtctg ctgaatctct cggcctgcat atcggtgaac aacagatgca gcacctgtta
    10261 cggggcggca gtctgtacgt tgacggcagc atcattgcac cgcagggatt tgaaattgta
    10321 cgcaaaccgg atacccgccc ggacagccga atcacgcagc tctggcagcg cctgagccgt
    10381 aatcatggcg taagcagcac ggagatccgc cataacccgg tcgccagcta tctgacacag
    10441 ctgggggcat cagaccctga agccgccgca cgcctggcat ccacacttca gcaggaccag
    10501 aacaccatga aaacacccgt taccgtgctt tctgacatgc tgcgcgccat ccgcgacgca
    10561 gagcacgcac agagaatcag tgaaaccact gaacgcgccc gccgcaaagc agacctgctg
    10621 cggggtggcc tgaccagtgg aaacaaaaaa cagacagaaa cgggactcac gaatcccgta
    10681 aatgagcaaa aaacgcgcag cgatatatga agcgcgcaca aaacaggcaa aaacgggatt
    10741 tcagaatccc gtaaacgatt aattaatcaa cataaggaaa agcgacatga aaatttgtat
    10801 cgacgacggc tccaccaaca tcaagctggc atggactgag aacggcgaac gccgcaacgc
    10861 catcagcccg aacagcttca agtcggaatg gtctgcgccg ttcggtggca tgcagcccgc
    10921 gaactacatg cttgatggcg tgcgctatgg ttttgatccg gtcagcgatc gctttgtcca
    10981 gacaaccgac acgcaatacc agtacagcga tgtaaatgtc attgccattc atcacgcgct
    11041 ggtcaaatca ggcatcacgc cacaggaggt ggatgtggtt gtcaccctgc cactgagcga
    11101 gtatttcgac acaaacgcac agccggacat ggccaacatc aaccgcaaaa aagcgaacgt
    11161 tatgcgcccg gtggagtacc agaacggcga agcattcact atccgtaacg tgcgggttat
    11221 gcctgaatcc attccggctg gctttaaggc actggctgac atgagtccgt ttgaatccct
    11281 gctgattgtg gatttgggcg gaaccacgct ggatgtggca aaggttcagg ggcaactggc
    11341 aggtatcagc caggtgtttt gcgatccaca cgtaggcgtt tctctgatgg ccgatgccgt
    11401 actgtcggtg atggccacta acggtatgcg caccagtcac cacatcgcca ataccattat
    11461 cgaacatcgc catgatgaag cctggctgcg ccagcacatc cacaatgacg cgcattacgc
    11521 cagcctgatg gcggttattc gtgaaaagga agaaacactg aaacaacgcg tgatccgcgc
    11581 gctggcgggt ttttcgggtt acgggcgggt gatggttgtc ggtggagggg cggagattgt
    11641 ggcacccgct atccgcgaag cctgcggagt taatgcgact ttcatcgcgg acggggtgcc
    11701 acagtttgct ctggttaatg ggctgtacgc aatggacaag gagtaaacca atgacgacac
    11761 caaccagacg gataagtttc tatctgaagc ctgcggccgt caagaacgaa agcgaagcat
    11821 gcgcctggct gggcagcctt acaccagaag cccgcaaaag cggccaacgc gtggcttttc
    11881 tggccgggct ggcacttctg aaaatgaatc cagcagaggc ttaccgactg gctgcatggg
    11941 ctgacgatga ggcgttatcg gtgacacaaa ccaggacaga acgccccgtg ccacagccag
    12001 taccaaccgc acagataacc agtcagatag cagggaatat ccgggcatta tttcctgaat
    12061 gacgaatttg acggctttta attttatcgg ggtgcactgt cgccccgcac gaggaaaaca
    12121 cgatgaaaaa atacgaatac atgctaatga aagaagcact gcgggctggt ctggctccct
    12181 gtcctgtatc cgtagaagct gttgttggga acacgatttt ccgaacgctg cgaaatcgcg
    12241 tatccgtgaa gatggttcac caaccaccca aatctaccag catgatctat ctgccgctgc
    12301 ccgaggctat caaacttatg cgttcgctga aatttgcgat ttggatgcag acacaaatca
    12361 acaaagcggt gcaaaacacc ttcgttaaga gactaatacc ggagcttgct gccagatgtg
    12421 caaaatgtac cagacccgca cttcaatttg atctgtcatt gattcaacaa gctacggagg
    12481 cagaactgta ccgcctgtca cctatccgtg atgctcacac ggaagcatca gaaatgttcc
    12541 ggctcatgtt tcaatttaat tttgccgttc gacgcacacg cgttcaggtc aatcgcatga
    12601 tgcaggagca ggaagagtta acacagtaca aagggggcag ccagtgagta attcagcacg
    12661 actacagctt ggtttttcac cgctatcaaa aaccatcgta ttggcaaaaa tgcgtgactt
    12721 aggagacgga acaaaacgcc gcgtaggaaa tgatcgcggt cgcgatgtaa ccaacgaggc
    12781 cgctcaactc gtttggcatc tagtcatggc agaaggcggt gaaattaatt gggagctgga
    12841 tgatggttct cgtatgattt tgagggccga gaagagtaaa tagctcaagg gggattaatg
    12901 ctaaatgatg atgaagaaga gctgcttatg caggaatggt cgctcggtga ttatgataat
    12961 ggtgaaaatg ggtgcccgca ttgtggccgt tatcgtttat gtatctgcca gaatggaaaa
    13021 cacagatgtg aaaagtgcaa ttggtctccg gaacttaatg actacgtgcc gatcgagtgg
    13081 tagaggtaag tcacactagg tgttttttag ttgattttat aaatgcacaa tagtgcacaa
    13141 atttgcacaa tttttttgaa cgactttttg cccttccggc ccgcgtggcg gctggatccg
    13201 tcaaggatcc gtgcatgcac aaaaaaacgc gttttttctg cgcgcaggtg acgggggaac
    13261 agcccgcgtt tcagggggta aatagcattc cctgaacgat gtcgcagaga tacaacagaa
    13321 tggctgtatt tctcacgctg agcgtgaaaa agacgtgagg gcttttgatt tgatggggtg
    13381 aaaggtaagg ccgtcaaaat cgcactgaga cggcgagaat atgcagtcaa cgaggtggga
    13441 ttgcgtaaga gtctgactgt cgatgatggc aatcagcagg aaagcgtcgt gaaattatct
    13501 gactgataca ggagctggag agtcggggca taaatttttt atgccccggc gaagcagcag
    13561 acaagcgaag cgcgtcagga tgtgggctgg gtgtctaaca gtgcgtaagg gttaaagcgg
    13621 atcacctctt cgccaagcca gtcattgatg tgcttcatgg cctccatgac gggcatcagc
    13681 tcgttaattg cgtaaacccg cgcggccttc tccacatcac caaacgcact tttttcgccc
    13741 ggcatcgccc ccatcagttg cggcggaacg cggtgcgcag ccagcacatc atcacgggat
    13801 gccgccttaa cattcatgaa ctcatccttt gcggtgatct gctggaacgg caaaatttgc
    13861 accccctctt tgcccccgtt gggcgcatga atgagcacgt ttttaaacgc accaccacca
    13921 cgtgccccct gtagcgtttc tttcagggag tccatgcttt cgcggtttac ctgcgctgca
    13981 ccgatgtaga tgatgcaccc ggcgtgggat ccgttgtcgt agtacagttt tctgaacatg
    14041 tccgccgaat gagaaaggct ggccgagagt aatgcgccaa gatattccgg catgccgtag
    14101 atttcctggt taatatccgg attcatcagg tggcacactt tgccaggacg aaactgaaac
    14161 gcatccttgc catcctgcac ataccaccat gattcaagat cgcttccgcg tcgcatgtat
    14221 ttcgccaggg cgtgccgtaa tttaagcggt tcgccgagca tattgctccg aagctcaagg
    14281 aatgcgttac cgaacacaaa ccagtccagc gccagcgccg agaaatcctg ccgggaaagc
    14341 agcgggtgcg gaatatagca gccgagcaat acattgcgct taaagtaaag cgcagactga
    14401 tgccaggatg tttgccgggc agctcttgcc agaccgtacc agtccaccgg ggtttcatac
    14461 caccgcccgt tatcagcaca gtacatattg tccagcaggt catgcccggt caggcggtaa
    14521 ggaccatcaa atgtgaatgc actgagcgac gattctttcc tgagcgcatc agcgagatca
    14581 atgcgtgaac tcatgcgcac ttttttattt tttctgctca tcagaactcc ataaccgtga
    14641 aacgctcgtt ttctccttcg ccgccaatcg gttcgttaat gacagcaagc atggttgccc
    14701 acgcaaggtc gccgtggctg atccccctcg cgcggtccgt ttcgtaagtg ataaaaccgc
    14761 ccggtgtttt caccttacgc acggcgttaa aggccgcaac cagctcgcgt tcggcgcgat
    14821 cgtattccca ccgcccggca cgcattattt gcagcatttt cagtaccagc gaccgttttg
    14881 atgacagcgt gaaggtgtaa gggatggcgg cgggaaaaaa ccgtttcact atctgataga
    14941 cggcatcccc gtgccctccc gtcgcatcaa tgccgatgtg ttccacgttg tagcgatacg
    15001 tgaactcttc aataactctg gcctgctctt caaattccag cccctgaacg cgtcgcgtct
    15061 ccaccgttct gaaacgacca ccaggaacag ccggaggaac caccacggac acagcgccgc
    15121 tgtcgccgtt accactgctg ccgtttgcgt catacccaat ccataccgga cgattcccca
    15181 tcgggcgggg ggcaaagggt ttccagtctt tccagtcgtc gtatccgtca acaccgcagc
    15241 caatcaggat attcaggtta aatgccgatt ccccttcgcg gacaaactca cacatataga
    15301 gattgcggaa ctcgtcttcg gtgttttcat cacgaatttc atcaatatcg gtgtgtttcc
    15361 agccgtgatt aaccacatct tccagcgtga caatttgccg ccacgtccgg tcagggcaga
    15421 taagcccgtt atgcagcgtt ttccagtcca cagaaaaacg ctggcgttta tgcgcggcct
    15481 ttttctcgtt ccagcggtcg ccgttccagt aggcgtatgc ctcgtgcgtt tcggtggatg
    15541 gcgtggagaa gcaggtgcgc cgcagtccgc tgagggttgc catagcgcca gccaccttgc
    15601 gcagttcagc aaagcgactg acccagaaaa attcatcaaa ataaaaattg cccgtgtaag
    15661 attgcgccgt cgcagcagaa gtgccgagaa aatgcatctc tgcgccgttg gagaggatga
    15721 ttttatcgcc ccctttcagc tccacatcga cttcagccgc ggccttctga ataatgcttt
    15781 taaactggaa cgcctgacga cgcgacgcag acaaaaaaat ctggttacgc tggtaaggtt
    15841 gtgccacatc gtcacgcagc gccatcagca gtgcttcctg tgcaaaatac caggtcgccc
    15901 caatctgtcg ggatttcagg atcatcctgt tacgtatccc ggcttccctg caaagcgtca
    15961 gggagtcaaa ccagccccgc tgatgccact ccagcctgct gatgattttt tcccgcagtg
    16021 cggcaatctg ttccggcgtg aaatgatttt tgagtttttt cgcccggcct ttctttcctg
    16081 tggccgtcgc atccggctgg ccatcatgca gttttttaag ctgccgggtc agcaggtcta
    16141 tttccttaaa gtcaccacct gttttattct gtttttcagt aagctggatg aggcgcgcat
    16201 cgatggactg cgtgacacgc tgcacgggtg gcgtttcatc ccactggtcg cgttttttcc
    16261 acgcataaat cgtgttcggg tttattccca tcagacgtga tatttctgcg ggcggataac
    16321 cctgccagta aagttgccgc gcacgctggc gcacaaaagc gtcctgaatc attgctcccc
    16381 ctgagtaatt acaggaagat tacccgcgcg cgaaaccgtt ctccttaacc ccctgttctg
    16441 gccgttttct tacaacaaaa gccctttgta tcagcctgtt acgctttgcc atcatgactg
    16501 aagaaccagt cagaggggca aaaactatgg ctaatgaaaa aaagacatcc cgcaaaaagt
    16561 ttcgcgtggc tgtctccgga tcaactgttg atggccgtga aatcagtccg gtgcatctgc
    16621 gtgaagccgc cgagaacttc aacccggatg tttacgctgc ccgcgtgaac gttgagcact
    16681 atctctcgcc atgcccgtca agcgaatttt ccgcaatggg cgatgtcacc gcactgagta
    16741 cggaagacat tacggaaggt ccgctggccg gacgtactgc gctgtatgca gaaatcgaac
    16801 cgaccgagcg catgaagcag cttgtcgcgg acggcaagaa aatctattcc agtatcgaac
    16861 tgcacccgca gttctccgtt aacgggcgcg cctatctggt cgggctggcg atgaccgaca
    16921 ccccggcaag cctgggcact gagcgcctga aattcacggc acagcaacgt caggcggtga
    16981 tgacgttcaa cagtatccag ggtgaagcac cgcttatctc cgaagccatc gagtctgaaa
    17041 tcatcgaaat ggcagaacaa cgccaggaag aaggcaccca gtggtttaac cgcgtaatgg
    17101 ggattattgg tcgtggccgc aaagcggatg acgccagttt ctcccgtatt caggaagcgg
    17161 tggaaagcgt cgcaacgtca caggccgaca ttatcgaccg ttttaatgcg ctggaaacac
    17221 gccatcagca ggacagccag aaaatcactt tactgaccac ggagctgaca gcactgaagg
    17281 aaaaactgcg cacgcaggac ggcgatccgc agaaccggtt caccgcaacg ggcgcagcct
    17341 ccgaccagct ggctgacttc tgataagaca aaggagcaaa ttttttatga atctggtgat
    17401 gtcagatatt acccgcaaca agctgggttg ctatatggcg cagcaggcgt cgcttaacaa
    17461 tatcccggtt tctgcactgg tatcgcgatt taccgtggaa cctgcggtgc agcaacgttt
    17521 tgaaaacgcc tcaaaggata gcaccgaact tacaaaaaaa attaacgtga tcggcgtgac
    17581 cgaccagaaa ggcgaaaaaa tcctcctgga caccacagga ccgattgcac gcacaaatac
    17641 cagttatgac ggaacaaaac gccgtaaccc gaataacgtg gttgatctga aaaaccgcaa
    17701 ataccagtgc gaacaggtga actacgacac gtttatttcg tatccgcagc ttgatgcctg
    17761 gtcggcacac agcgattttc agtcccgcat cagcgcacag attgcccgac aggtggcact
    17821 tgaccgcatc atgatcggtt tcaacggcac atctcacgcg gatgagtcca acttcagcac
    17881 caacaagctg cttcaggacg ttaacgtggg gtggctggag cacatcagaa ccaatgccag
    17941 cgagcgcgta atgaatgacg tgacgctgac ttcccgcaac atggacaaca ccgtggcgca
    18001 cgcgggtaaa tacgcgaacc ctgatgcact ggtacaggac gcgcgctcat ctctgctgga
    18061 tgaatggcac aaggaagctg acgacctcgt ggtgattatg gggcgcaacc tgtttaactc
    18121 gctgcgtctg ccagtgctga acagcatcag cggccagaat cccaatgcgg aattacttgc
    18181 cgggcagctc atcctgtcat cgcgcaccat tggcgggctg ggtgtgttcc ttgcaccgtt
    18241 cttcccggat gcaacgatgc tgatcacctc attcaacaac ctgtcgattt actggcagaa
    18301 aggttcaatg cgtcgtctga tgaaagacga accggaatac aaccgcatcg ccacctacca
    18361 gtccatcaat gacgcttatg tcgttgaaga ctatggcaag tgcgcgatgg tcactggcct
    18421 gaagttcgcc gacagctaat caactcacgg cgggcatcat gcccgcctgt aacggagaga
    18481 aaaaatgatt actcctgcac agcaacactg gcagaacgtg atggcacagc gcgcaggccg
    18541 ggcgaatgaa ggtgtggacc acgccgcgcg taccgcgcat gaagaggtgc tgtatcgtct
    18601 gcgtctggca caggcccggc ttaagggcgt acaggccaga agcgcgaaag ccgacatcaa
    18661 aaaagcgttg ttgccggact tttccggctg gattgaggga acgctggagg ctgacggcgg
    18721 gcaacaggac gaagtgattg ccacgctgat ggtgtgggcg attgactgcg gcgatcttcc
    18781 gcttgcgctg cgtattggtg cgtatgcggt ccgtcacaac ctcatcatgc cggataactt
    18841 tggccgtact gctgccacag tgctgaccga agaaatctgc aatccggtac tgacgcaggc
    18901 cgggacggat gccgacgcgg atttgtccgt ctttatcgaa ccactggaca cactttggga
    18961 aattgtcgcc aaccaggata tgccggacga agtgcgcgcc aaattatgca aggcgtgcgc
    19021 ctttgcccgc cgtggcctga gcgatgcgga cagcatggcc tcatcactga agctgctgcg
    19081 cgaagcgatg catctgaacc agaacgcagg tgtgaaacgc gagattgcaa ccctttcccg
    19141 cgccctgaaa aaagccgatt ccgcagccgc accagaagac gccagcgcac agcaggcgca
    19201 ggacgaaagc agcgaaagta aaaagacaac gcggaagcct gcaacacgaa aaaccaccgc
    19261 gacgcagaag gcgaagcgcg gttaacgact gaccccgtca gcgggcggcg tgcgcggtgt
    19321 tccggtttgg ctccgtgacc gtttacaccg cgcacccacc gcccgatttt ttcaggagtg
    19381 aaccccatga gtatggttgc cagaactgaa cccggacccg cagaggacga catcaccgac
    19441 accgaagatg gcgatacccg tatttcagcg ggtgcattct ggccggatat tgtgctgcgc
    19501 gagctgcgtc tggcggtacg actgccgggc cgcgtgacca cctcccgcct gctgcatacc
    19561 gccaccgggg ccgtggcaca cgttacccgc gagctggaag catggcagca ggaacagcag
    19621 gcggctggcc atcagacgct ggccgatgtt ccggcacctg taattaacgg agaaagcgtc
    19681 aatctctggc actggcgcaa tgctgtttat accgccacac gcgccctgat tctggagcgt
    19741 taccgcgatg cggacacaac ggacaagggc gaccgccggg cggacgcact ggatatacag
    19801 acatcggatt tgtggcgcga tgtgagctgg gccatctctg acattctgtg ccgcccgcga
    19861 atctttgcgg agttgtgctg atgaaagtga agacactgga aggcgacacc gtggattcgc
    19921 tctgtttccg gtactacggc acgacgcagg gcgtcaccga aaaggtgctg gatgccaacc
    19981 ccggactctg ccgacaggta tttctggaag ccggacagga ggtggaaatg ccggagccgg
    20041 agaagaaaaa acaacgagag atgatacagc tatggtaaca ggtttttttc agcgggtgag
    20101 tgactggatc accttctggg catcagtcac agccaccaca ataggcgtga tgaccatcag
    20161 tgaaaaaatc gcccttgccg ggttgttact cggttctctg tctgcattac atgcctggcg
    20221 acatcgccgc ctgatggaaa ccgccatgca gcgtcgcaat gaactcatcg ccagtatcct
    20281 ggcgcagtca gacgcacggc cacttcacga cagtgagcgt caggctctga acattctgcg
    20341 ggataacgat acacatgata aagctgcccg ttaaacgtta cgccgtagcc gccattgtgg
    20401 cgctggggtt ctccattgcc cctcacaaac tccggaccag cccggaagcg caaatccgca
    20461 ttgccacatg ggaggactgt cgcgcaacgc cttaccggga tattgccggg gtaatgaccg
    20521 tggggtgcgg ctcgaccggg catgtggaaa atcgtctgta caccgaaacg gaagtcgccg
    20581 ggcgctgggt taatgatatg caacatgcag aaaactgcat aaatcagaac ttcagcggca
    20641 acgccatgcc acagtccgcg tttgaggcaa tgacagatgc cgcctttaat cttggatgca
    20701 gaaatcttat gtggttcagg gataagaacc gtaactacca ccgcaccact atctggaaac
    20761 atgcacaggc gcacaactgg cctgacatgt gcaacagact gactgacttc gtgaacagcg
    20821 gcggcgaacg ctcacaggga ctggttaacc gtcgtactga ttttcgtgca tggtgcttac
    20881 gtgacaccgg aggccagtca tgaaagtgcg ttacatcctg atgatggcac tgaccgtcct
    20941 cacgctgctg accataacac tctggcagac attcagactg gggcaggaag acggacacaa
    21001 ccgttataca caggcgcaac tggacgatta cagccggacc ctgaaagagg ccggcaccat
    21061 catcagcacc gcatccggtg tactgcgtga cgtcatgaca gcgcgccaga accgacaaca
    21121 ggaaggggaa atccgacgtg aacagctacg caacgatatc acgacagatg catgtgcccg
    21181 cgctgttcct gacgctcgct ttactgagcg gttgcaccag cacgcagaac gcgtccgcgc
    21241 cgccgccgtc gttccgtttc atcccggaag ggctgaccgc ccccgtactt cccccgccgt
    21301 ttccggtgac gccatcatgg ggaaacatcg ggatatggag cgacagcctc atggacgcac
    21361 tggaaacctg taacgccgat aagcgggcca ttgaattact ggaacagcgc aggctgcaac
    21421 gactgaacaa cgaggacaac aaccatgctg aaaactgatt ccctgcgtga agccatgacc
    21481 cgttcatgcc ggtggtgtca tgccaacccg gaaaaattca ccattttcgt ggagagcggc
    21541 aacattgaaa cgaccggaga aaccccatcg tttgtttacc gctatcagat agtgatgttt
    21601 gtcatggatt acgccggaga gctggacgac ctcacgctgc cgctgctggc gtggttatcc
    21661 gaaaatcagc cacagttgtt gctcaatccg gagcgtaatc aggacatcaa attttccgcc
    21721 gttatcaatg acgatgacag cgccgatctc ctgtttacgc tccccctgcg ggaacgcgtt
    21781 cgcatcacgc gcagcagtca gggcacaccg caggcagaac acctgccgga gccaaaaccc
    21841 cgcctgccct cttccgaagg cgactggtcg catgtattcc aggatgtgac gtggggggaa
    21901 agcgatggat aaggcattca cccgcgtgga tgaaaccttt gaggctatcc gcgacagcct
    21961 gaatcagcag gccattaata acatcgccag aaagctggca caggatttac gccgcgccca
    22021 gcaggcgcgt atccggtcac agaaagcgcc ggacgggacc gcatggacac cacgcagacg
    22081 ccgcgtaacc cggatacagg agcgcattcg ctttatctgg aataacgaag cacgcacgct
    22141 gaaaaactgg catcacgaca cggggaaata cgggcgaacc atcaccgggt gggatgagga
    22201 taaaaacaat atccgcacgt tttaccggga tgacatcgac cgttttctgg aaatacgcac
    22261 ccggcgcatc aaccaggaca gcaccaggcg cgtctccatg ttcgtaaaac tgcgcaccgc
    22321 ccgctacctg aaagcccgtg cagatgcttc cggtgtgacg gtgggttaca gcggcgtggc
    22381 cgcacgtatt gcacgcgttc atcagttcgg tgagcgcgat caggttgcgc cgggcatttt
    22441 caccgattac ccggtacgtg agctgctggg tatcagccag gcagatgagc gcctgattta
    22501 taacacggtg ctgggccgga ttgcggaggc tgtacgatga gcgcagaact catgcgactg
    22561 ctgagcaata tcatccgcac cgggatcatc tctgaagttg atgaggaatc ctggtgcgtg
    22621 cgcgttcgca gcggcgaact ggaaacaggc tggctgcgct ggaacaccac gcgcgcggga
    22681 gacttcaacg tgtggtttcc gccatcacca ggcgaacagg tggcaattgc ctgcatcggc
    22741 ggcaacccgg aaaccgccat gataatcggc agcctgtgga gtgatgccat tccggcaccc
    22801 ggcaaaagcc tgaaagaaat cgtggtcagc gcgccggacg gcgcggtgtt ccgctacgac
    22861 gcggacgcag gcgcactgag cgcaagcggc atgaaaacgg ccactttgca ggcatccatc
    22921 agcgtgacac tgaacacgcc tgtcgtggaa tgcacagacc ttctgagaac agcgacgctt
    22981 gacgtcacaa aagggggaac gatgagcggc aatatcacgc acagcggcgg cgatttcacc
    23041 tcaaacggca tcacagtgca tacgcataag cacggtggcg ttaaaggtgg cagcgattcg
    23101 acaggaggcc cgcagtgaca acccgctaca caggaatgaa cccggacggg acgggaaacc
    23161 tgaacgatat ggcgcacctg aaacagtcag tcagggacat cctgaccacc ccgctggcaa
    23221 gccgggttat gcgacgggaa tatggcagcc ttgtgcctga tttgattgac gaacccatga
    23281 ataacaccac gcgtctgcaa tgcatgagtg ctaccgtgat tgcactgaca cgatgggaac
    23341 cacgcattgc cctgaacgcc atcgacgttg tctggaaggc aggaggccac gccggggtga
    23401 cgctgtcggg cactgtcatg cagaccatgc agaatgttga attaaccatc acgctgaggg
    23461 agtaaatcat gcctgccgtt gacctttccc agttaccgga acccgccatc atcgcggagc
    23521 ctgactttga ggcaattctg gctgacacaa aggccatgat gattgcagct tatcccgccg
    23581 aacagcgtga agtcgtctcc gccgcactgg aactggaatc ggaacccctg aacgttatcg
    23641 ctcaaaccat gtcgtttcgt gaaatgctgt tacgccagcg ggtcaatgag gccgcacgcg
    23701 cctgcatgtt gagccacagc gccggaacag acctggacaa cctcgcgggc aatatgaaca
    23761 caaagcgcct ggttatcact ccggcaacgg ataccaccga cgtggtgatg gagaacgaca
    23821 cctcgctgag actgcgggcg cagcgggcgt atgacggcct gagtgttgca ggcccgtcag
    23881 gcgcatacga gtattttgcc cgcagcgcca gcggtctggt gcgcgatgcg cgggccatca
    23941 gtccgtctcc ggcaaatgtg acggtttcca tcctgtccac tgagggcgac ggcacagcaa
    24001 cggaggcgtt gcttaatacc gttcgcgccg ttctgaatgc agaggatacc cgcccggtgg
    24061 ccgaccgcct gaccgtacag agtgccagaa tcgtgacatg gcggctgaat gcaaaactgt
    24121 acttttaccc cggtccggaa tccgaaccta ttctggctgc ggctgaatcg tcgttcagga
    24181 agtggctggc tgagcagggg cttatcggtc aggacgtggc gttgtccgcc attgctgccg
    24241 cactgcatgt gcacggtgtg caacgcgtgg agataatcga acccacacag aatatggcca
    24301 tcagcgacat acaggcggcg cgctgtgagt cattcaccat cagcgaaggt gggcgtaatg
    24361 agtaattcac tgttaccacc atcagccagc aatttcatgc gttgtgccga agccgtcgga
    24421 acacgcatta cagacattcc ggcagacctc aacacgctgt ggtcgccgga cacctgcccg
    24481 gtgcatctgc tgccttatct cgcctgggcg ttttccgttg accgctggga tcgcaactgg
    24541 ccggaagaga caaagcgaca ggtgattcgt gatgcatggc tgatacaccg acacaaagga
    24601 accatcagcg ccctgcgacg ggccattgag ccgctgggat acctcattcg cgtgtctgag
    24661 tggtgggagt tcaacggcga acccggaaca ttcaagattg atgttggcac gctggacagt
    24721 ggcgtgacgg aggaaatgta tctggaaatg gagcggttga ttgctgatgc ccgcccggtc
    24781 agccgccaca tgacagggct gaatatcatt caggagattc agggagatat tttcgcggca
    24841 gcagcaactt acgacggtga agtcattacc atttatccgg acgattaagc atgagtacca
    24901 caacacgaaa atttaaaacc attatcaccg atacgggtgc aaaaaaatta gcgcaggcaa
    24961 ccgcgccaga tgggaaacct gtccgcctga cacatatggc cgtgggtgac ggcggcggta
    25021 ctttacccac gccagacagt aagcagaccc gtcttgtgca tgaggtgtgg cgacataccg
    25081 ttaatcgcgt caccctggac gcaacacatc agaaccacat tattgcggag ctggttattc
    25141 ctcctgaaac gggcggattc tggatccggg aaattggtgt gtttgatgag cacggcgatt
    25201 tgatcgcggt aggcaatact gccgaaagtt acaagccaac cgttgccgaa ggatccggac
    25261 gtgcacaaac atttcgcacc attctgaccg tatccagcac tgccactgtg gcgcttaccg
    25321 tggataacac catggtgatg gccacagtga attacgtgga tgacaaactg aaagagcatg
    25381 aacagtcacg acgtcacccg gacgcctcgc tgaccgcaaa aggccttgtc cagctcagca
    25441 acgccaccaa cagcacgtct gaaacgctgg cagcgacacc aaaagccgtt aaggcagtaa
    25501 tggatgaaac gaacaagaaa gccccattaa acagcccggc actgaccgga acgccaacaa
    25561 caccaactgc gcgacaggga acgaataata cccaaatcgc aagcacggct ttcgttatgg
    25621 ctgcgattgc cgcacttgta gactcttcgc ctgatgcact gaacacactg aacgagctgg
    25681 cggcggcgct gggcaacgac ccgaattttg cgaccaccat gactaacgcg cttgcgggta
    25741 agcaaccgaa agatgccact ctgacggcgc tggcggggct tgctactgcg gcagacaggt
    25801 ttccgtattt tacggggaat gatgtcgcca gcctggcaac cctgacaaaa gtcgggcggg
    25861 atattcttgc gaaatcgacc gttgccgccg ttatcgaata cctcggttta caggaaacgg
    25921 taaatcaggc ttctggcgca ttacagaaaa accagaacgg cgcagatatt ccggggaaag
    25981 ataccttcac caaaaatatt ggtgcctgcc gcgcatatag ctcatggctg aatattggtg
    26041 gcgatagtca ggtctggaca accgcgcaat ttatttcgtg gctggagagt cagggagcat
    26101 ttaaccatcc ttactggatg tgcaaaggct catgggctta tgcaaataat aaggtcatta
    26161 cagatacagg ttgcggaaat atttgtcttg caggtgctgt ggtggaagtt attggcactc
    26221 gcggcgcaat gaccatacgc gttaccacgc cgagcacgtc cagcggtggc ggaattacta
    26281 acgctcaatt cacttatatt aatcatggtg atgcttacgc tcctggctgg cgacgagact
    26341 acaacacgaa aaatcaacaa cctgcatttg ctttagggca aacaggaagc actgtcggaa
    26401 atgataaagc tgttggctgg aactggaata gcggggtcta taacgcaaac attggtggcg
    26461 catcgacatt aatcctccac ttcaatatga atacggggag ctgccctgct gtacagttcc
    26521 gcgtgaatta caggaacggc ggtatttttt atcgttcagc gcgtgatggt tatggctttg
    26581 aagctgactg gtcagagttt tacaccacaa cccgcaaacc ctctgcggga gatgttggtg
    26641 catatacgca ggcagaatgt aactcaaggt ttattacagg tattcgcctt ggtggtctgt
    26701 tatctgttca gacatggaat ggtcccggat ggtctgacag gtcaggttat gttgttaccg
    26761 gttccgttaa ctcaaacaga gatgaactaa ttgatacaac tcaggcaagg ccaattcagt
    26821 attgcattaa tgggacgtgg tataacgcgg ggagtattta acgatgatgc acttaaaaaa
    26881 catttctgct ggtaatcctg aaacaaaaga gcaataccag ctaacgaaac aatttaacat
    26941 caaatggctt tatacagagg atgggaaaaa ctggtatgag gaacaaaaga actttcagcc
    27001 tgatacgttg aaaatggtct atgaccacaa cggcgttatt atttgtattg aaaaggatgt
    27061 ttcagcaatt aatccggaag gcgcaagcgt cgttgaggtt cctgatatta cagcaaatcg
    27121 ccgggctgat atttcaggta aatggatgtt caaagatggc gtagtgataa agcgaactta
    27181 taccggggaa gagcagaggc aacaagcgga aaatgaaaag caaagcctgc tacagctcgt
    27241 cagggataaa acccagctat gggactcaca gctacggctg ggcattattt ccgacgagaa
    27301 taaacaaaaa ttaaccgagt ggatgctcta tgcgcagaag gtcgaatcca cagacacctc
    27361 cagcctgcca gtaacgtttc ccgaacaacc agaatgaaac aaggcccgat atcgggcctt
    27421 aatttttact caggcttttg tggccattct ggctttgccg tatccacacg gctgaccaga
    27481 acactgtagc gttcccatgc ttccagtcgt gtgcgttcct cgtccgtcgc catattcagc
    27541 ctgacagcgc gttccagcgg ctggatgact gattcagctt cggaaagcaa tgcggccttt
    27601 tgtgattcgg cctgttgttg ctgttcgtca gccgtataaa tccgtttaac cacagctccg
    27661 tccttaaaca tccacttacc ggaatcatca gcacgacgat tagctgtaat atcaggaacc
    27721 tcaacgacgc tataaccttc agggttaagc gtggaggcat ctttggtgat ggcgacaata
    27781 atattatttg catcgtaaac aatctttatt gtgtctggct gaaagttctt cacttcctca
    27841 taccagtttt ttccgtcctc agagtaaagc cagatgactc cgtgcttctt tgttaactca
    27901 tactgctcca ctgttttagc attacctgct tttatgttct ttaagtgcat catattaaat
    27961 actcgccaca ttataccaag tgccatttat atacttttgc actggcctgt aatgaacgcc
    28021 tgcaatatta tcggcagagt tttttccggt atcctgtacg ttaatccccg tcagtacata
    28081 gcctgacggg cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga
    28141 accaagtcga acatctttca catatctgga atcaaaattg ccatagttgc cgggaataac
    28201 ttgcgagccg caaagccagt tcccgttatt atccatgtac gcctgaccat cggtgccatt
    28261 ggctgtcctt gagttattaa tcatgtagat gccaaattgc ttatttccca gaccgccaat
    28321 cataaatttg cggtcggcat ggtcctgacg gagcaaagcc tgagcaccat cagtggatac
    28381 cgcattacgt cccaaaataa cattctggtc acgcatatga atccacatgc cggtactact
    28441 gttaattgca aaacggtttg caaatatatc ccctgtaaca tcaagaccat gccccatgct
    28501 tatccggcca gttctgagat taagcgtaaa ggggcgtagt ggccctatat taccattttc
    28561 cccttcattc tctcgtgtag ggatgatatg caggcattct tcagaacgac gaaaaatggc
    28621 accaaaagat gaattaaata tcctcagtgc attgactgtc gatattttca cttcactgct
    28681 gaaaagggct tcaacaagaa cagacaaagc attccattta agattcatca ggtctttagg
    28741 ccgggtgcca atgatgcggt gtctccattt gaaatattca ttgccgttgt tgcctgtttc
    28801 aaaccacatg taggaatcgg tgtctgcatc cgaattattt ttaaacccaa tctttgccca
    28861 gtcagtattt cgaatccagg caaggattga gtcgttttca aaagtaagtc caccggacaa
    28921 cgtatcgcca tttttttgca cggcgttacc agccttgttt accgtttcct gtaaaccgag
    28981 gttttagata atggccgttt ccggcctgca tggcatgatt tgcgcttttg gacgggagat
    29041 tcagcgtgct gattggctat gtaagggtat caacaaatga ccagaataca gacctgcaac
    29101 gaaacgctct tgtttgtgca ggatgtgaac aaatatttga agataaatta agcgggacaa
    29161 agacagaccg accgggatta aaacgcgctt taaagcgcct tcaaaaaggt gacacgctgg
    29221 ttgtctggaa actggatcgc ctcgggcgaa gcatgaaaca tctgatttct ctcgtcgggg
    29281 aactacggga gcgaggaatt aattttcgca gtctgaccga cagcatagat acatcttctc
    29341 caatggggcg ttttttcttc cacgtgatgg gtgccctggc tgaaatggaa cgtgaattaa
    29401 ttgttgaacg tacactggcc ggactggcgg cagcacgcgc acgggggcgc acaggcggac
    29461 gtcgaccgaa gctgacaaaa gaacagcatg agcaaatagc aaggctgatc aaaaacgggc
    29521 acgacagaaa acaactggca ataatttacg gcattggtat atcgacgatt tatcgttacc
    29581 accccgcagg agaatcaatc ggaacaatag agaagagtca ggaaacaaaa taaccgctaa
    29641 tctgaccatt agcggttttt gtgttaaatc agaacaaccc tttaactgaa ctggccgcgc
    29701 tgttaaggga tgatgttacc ttatctttga agccggacag catatcgctg aacgatgagg
    29761 attgcaggcg ctcccgcaaa tcctcatcac agcgttcaag agtcagtgaa aattctatct
    29821 ttttcgcctt accgtagcga tcaaactcgg agcgggtcgt attcgtttcg gtcaggacat
    29881 acatgccgta aatctgcccg acgccatcaa tcagaggcca gggtcgtcct gtatacgcct
    29941 gcgtggtcag cagcgacagc gacacttcgc cacctgtaat ttcaggataa agcacaccag
    30001 aaagaacgat gcgctcatca cctgcaccga tatactgcca gcttgccgaa cggttaacgc
    30061 gttcattttt cacatgccgc cagcttttgt tttgctgtaa ctgctgatgc ggcaatgtac
    30121 gcagctcaaa aacaaacatg ccgtagatca tcatcatggc catgactcct caatctttat
    30181 cgtaaaaact gccacgcccg gcacgggcgc gccgttccat ttctgccctg accatttcac
    30241 cgaccagttt cgccagttcg cggggattct gcgtaacaac gttatgcaga tgaacatgaa
    30301 tttcaccacc aaatccggag gcaacaggct cccggttacg ggaagttaca ggaactgatg
    30361 ccactggaga gcgtatggcc tccgccaccg ggcgggagct ggccgcaaca acagggacca
    30421 gcgccggagg cagcggagcc gggaccacgg gtgtgatatt aattgcgggg gcaggcttac
    30481 tgacctgcgc aatcttccgc tcctgccact ccccacgaac agcaagtgcg cggggcaggt
    30541 tcttaaagac aatatcgccg gggccaatgc gttttttcgt ctcatcaacc agcttacctg
    30601 tgttatcagc aattttgctg agtctgcgta gcgtcccggt attgctgtct gtgagcggtt
    30661 tgttgtcttt gggtttatca cctccggtgc cattgccatt ttccacaggc ttcggcggat
    30721 tgattttcgc caggtccccc tgaagcaagg caaccttgtc ctgaagaatg gccgcacgct
    30781 gtgcgtcttc gattttcttg cgcgcccttt ccgcttcatc cggaaggacg ccaagttttt
    30841 caagtatcca cgccagcgta tccagtagca tttttgcagg tgtcagaaca agttgtaacg
    30901 caccgccaag aacgttaccg aatatctcgc cagcactggc acatttatcc agcgtttcct
    30961 tgctggactc catcggtgac agcagcgatt taaaccagtt aaagacctga ataatcccat
    31021 cgcgcacgac atcaaaaaca gggccaaacc gttcaaaggt ttcgcgcaac ggggtcagcc
    31081 tttccataat cccgctgaac accccggcaa aaaatgccct gatgggatcc cagtatttcc
    31141 agataagaac ggcagctccg gcaagcgcag ccacgataag actaaccgga ctgaacagcg
    31201 ccccgatagc gccaccaagt aaagaaacgg aacccgtcac cattccccat aatgcaggca
    31261 gaaccctgac aacattcatt gattcggtca ggagagaaaa accgagacgc aacgtagcca
    31321 gcttcccgtg aagcacccca ataaccagag acaacgaacc aaccgttgct gttgtcgcca
    31381 gcagtgcgcc tcccgctatc agtaactggc gcgtcagtgc cgggtgggcc tgcgccagcg
    31441 ccgtcacctt tgagacaacc cgcgtgagcc actgcgtgac agaacgcagc ggaccgtcaa
    31501 ccagatcact gatgcgaata cgcagacctt cccatgcact gccgagtgat ttcaggttgc
    31561 catcaaggtt attggccata acctttgccg tgcgttctgc ctcaccgcgc gcgccttcaa
    31621 gttcttttct cagtttggct aagaagccgt cacctgccgc atcaacgagg cccataaatg
    31681 atgtgaaagc ctcttctccg gcaatgtcct taaagaacga tacccggtca acctccccgt
    31741 atttgcgggt ggctttatag agatcggcca gcacattctc catcgggcgc attttgcctt
    31801 tggcgtcagc aactgacacc cccagctctt tcagtgcttc tgccgctgcc tttggcggtg
    31861 atgacagatt ggccaggctt ttacgcaacg ctgtacctgc atcactcccc ctgataccca
    31921 tcttcgccag cacgcccgcc atcgctgcgg cctgctccag cgatattccc agcttacccg
    31981 ccaccggacc tgcatatttc atggtttcgc ccagtgcgcg aaggtcagtg ttggtacggg
    32041 taaacgctgc ggtaagtgtg tcaccgaccc ggtccatctg gtcagcagag aggccgaact
    32101 gcgtcaggat attggaacca atatccgccg tctcgccgag gtccataccg ccagccgttg
    32161 ccatgctcag cacgccggga agcgcagcct gaatggcctg cggtgtgaaa ccagccattg
    32221 caagaaatgc ctgtccactg gcggcatcgc ctgcggtgaa ctgcgtttca gagccaagtt
    32281 ttaacgcctg ctcacgcagc gccttaaact gcgggctgtt tttatcgatt cgcgtcagtg
    32341 cctgaacacg ggacatctct ttcccgaacc cgatagcggg ctgcaaaaaa cgcccggcag
    32401 catagccgcc cgctgccgcc gcacctgttg ccagcgcacc acctgttttc agttttcccg
    32461 ctgtttcctg cgcgcgtgaa taccgctcac gcgcctgcgt tacacgcgca agcgcccgcc
    32521 gttcgcgttc aagctggttg ttgtattgtc cggtgcgtct gatggcctgc tggatggtgt
    32581 tatcgctgcc tgtcagggaa atgccgtggc gtttcagctc tccgccaagt tcccgcattt
    32641 tctgaatttc ccgtgtgcgc gattcattca ggcgttcaag ccgggtgctt aactgctgca
    32701 tcagcttttg ttgtttttcg ctgagcactg tacccgtgcg ttgtaactga ttaagggcgt
    32761 taagctggcg tcgtgctttc acgatgcccg catccgcttt actgacagcg tcacgggcgc
    32821 gctcaaatga acgcgcctga cgctcgagat ttttgatcgc cccctgcgtt cgctggatgg
    32881 agtcaccaaa ctgccccatc aggcggcggg cgttttcggc aggccgggtc agcctgtcaa
    32941 cggcgctgaa agcgacccgg atatcaagag tcttcattgt ctgcattccc gctgcgaagt
    33001 gccgcccgct cacgccagct aaccacttcg ccgggcgtca tcatgaagat ttcggcgggc
    33061 gaccagttaa aaatggcggc aatatccgcc acaaagtctt ctatgtgctc aaagcacaca
    33121 accgtgatca ggcttccgtc gcctgttcgt tcttctcgcc agagtccgca ccgctcaaaa
    33181 aatttacggc aaccacacat aactgaataa agtcacggga tgccattttt ttgatcgtca
    33241 cttcatccag tcgcggtgat gtcacgcgtg acagcagcgt aaacatggat tccgctttca
    33301 aattcagcac atcagacagc gacaaatctc gcagagatcc agcctgctca atagccccgg
    33361 tgatctccac atacgtgatt ttttcgccgc ctcgctcaat tggttgggta agttttacgc
    33421 cacgctcact ggtttctttc acagtgtcag caacgaccgt gttttcggta tcgatgtttt
    33481 tcgtctcttt catcaggaaa ctcctttcag tcagaggcga cgcactgcgc cgcctgcata
    33541 ttacttatca gccaagcccg agcgcggaac ggatgcgatc gggcacaatg tccctgccgt
    33601 ccttccggta aatgaagttc agcaggtcaa tctcccacaa cgggcgatcg ttaacactca
    33661 gcttgtagta ggtgttttta atggcgtaag tgtgtgatgt ggcttcgccc tgtttggctt
    33721 cccccatatc aatttccgtc acacgtccgc gcatttcgac ttcatacagg tcgctttcag
    33781 cgtcggtgta gtattcaccc gcaaaacgca gcagcgtacc gtcaatcgtg ccgccatact
    33841 taaggaacag ctcacgaact gcgcccccca tgacaaagct cgcatcaagc gcggagtcat
    33901 ccaggccgag atcaatactt accgctccca tcatgccacc accccggtag ctgtcggttt
    33961 tgcgcgtcag cttaggcaga gtgacggacg tcaccttacc cacttcgttt tcaccatcca
    34021 caaacagcgt aaaaaagcga agatgttttg gtacagccat caggcacctc ccagcaccgc
    34081 aaatgcggga ccaaagaatt catcagtaaa cgactggtaa agctccatgt cttccagcgg
    34141 gggaacaggc gtatatttgt agcgaatacg cacgcgcccc tgacgtaaat tcgtggtgcc
    34201 gttatccacc acgtcatacc agcacgacgc cccaatcagt ttcccggcag taaccagtga
    34261 atccagtttt gccctgatgg caccgataac atccttcacg ttcgcaggcg tcagtggact
    34321 gtcgatggtt tcaaactgcg cttccgcaat tgaatcagcc agcacctgtg cggttcgggt
    34381 atacacctca aagatgtagg cgttcgtttc cggtgtgcgg ttgccccaga agcggaaccc
    34441 gttgcgacga ataatggtcg tgatttcttt gttgttgagg ctgttggcat cactgtcttc
    34501 ggcctgcaac gaccagaaca catgcctgga catccccagc acatttttaa ccggaacgtt
    34561 ggacagcgat ttgtgccagc cctgctcatg gtcaatgtac gcacgaaggc cgcacgcata
    34621 ggcaggcgcg gggaacgttt cgtttttgcc acttttcggg ttgtaggcga tgaagtccgg
    34681 ccataagagc atcacctcac gttcgttgaa tttctggcgg taggtaattg cctcagccat
    34741 cgtgttacag tcatgacatg aggcatacac aaacgcgcgc agtttacccg caatcacgca
    34801 cagggatttt gtcaccgcct ccgtgtccag ctccggcgcg gccagaatac gcggacggta
    34861 tccgatgctt tcatcctgct ctgcaacaag cagcgcatac atccccgtat agctgccgtc
    34921 agattcagaa ccaccgataa ccagttgatc ctgcgttttt ccgtcttctt ctttgtgttc
    34981 agccacgcga acgacgatca cctttgtgct cacctggtct gcgatggcct taagcgcacg
    35041 ataaagcgtc cccgttgttc cgcattttcc cagcacgtca ttgacgcggg gcagcagtgt
    35101 gggcttgttc agcgggaaca gctccgcgtc cgcatcatcc gccgttgcca cgataccgat
    35161 aacgctggaa tcaacatcgt taatcgctgt taccaggtcg gtattttccg taacacgggc
    35221 accatgaaaa cgagtttcac tcatagcttc aaccccttgt atccgttaaa tgattcggca
    35281 acaatcatca cccaccacgc gcgtaatctc acccctgcgc cattctcccg ccacggcgac
    35341 aacaaaaagc agtaaccccc tccgcacgca catgcgacca tgccacacag ggagggagca
    35401 gatgaccgac accaccatgc aattgctcag tcagggcaca gaccccgtga aaatgccgga
    35461 ttttgatatt ctcgcggagg gtaaaacgct gtcaggcgtg gcagagcgcc tgatgagcct
    35521 gtcactgacc gacaaccggg gatttgaggc ggaccagctc accatcacgc tggatgatgc
    35581 ggatggtcag ttgcagctac cgccacgggg cgcgcgcctg acggttctca ttggctggaa
    35641 gggagaaccg ctgacagaaa aaggcactta cattgttgat gaaatcgctc acgaaggacc
    35701 gccggacagg ctgactgttt cagccagaag cgcagatttt cgggctgaat ttaacgttaa
    35761 acgtgaggtg tcctggcatg atgtgaccgt tgagcgtgtg gtatccgcca tcgctcatcg
    35821 gtatggtctg aaaccgcaaa tcagcgaaat gctgatggat atcgaaatcg accacgccga
    35881 ccagaccgaa gaaagcgaca tgtccttcct tacgcgcatg gcggaaatgc tgggcgcaat
    35941 caccacggta aaaagcggta atctgttatt catcatgccc ggtggtggcg tgaacgcaca
    36001 gggccagccg ttgccctcgt tcgccatcac acgcagcagc ggcgatcgcc atcagttccg
    36061 cattgctgac cgcgaagcgt atacgggggt acgcgcttac tggcttgatc ttaattacgg
    36121 gaaaaagaaa aaagtcagcg tgaaacgccg caaaccgtca aaaccaaaaa aggagaaaag
    36181 cagcagccgg gaaggcgact atatggaagg tgcggaaggc aacgtgtttg tgttacgcaa
    36241 aacttatcag aacgagcagg cagcaagacg tgcagcggcg gcaaagtggc agcagctaca
    36301 acgcggagcc gcatcattct ccatcacact ggcgcatgga cgtgcagaac tctaccccga
    36361 aatgcatggc acggtaacag gatttaaaag cgagattgat aatcaggact ggattattgc
    36421 aaaagccgag cacaccattg ataacagcgg ctttaccacg cagcttgagc ttgaggcaaa
    36481 aatcccggaa tggatagcag aaacagagtg agcaacttaa atgtattaac tcagaacgga
    36541 tcaacacact cacaacatag agtcaatata gctgctcgct ttgttccagc aaggatgcct
    36601 atttctaagg aaaaacagag cagaaacata tggtgttttc ctacctgaca tcaggggtg
//
LOCUS       DAQSWQ010000036        35777 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.036, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000036 DAQSWQ010000000
VERSION     DAQSWQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.036"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(1..581)
                     /locus_tag="R8G18_001836"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="R8G18_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="R8G18_001837"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="R8G18_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HEE9795555.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="R8G18_001838"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="R8G18_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HEE9795556.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="R8G18_001839"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="R8G18_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HEE9795557.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="R8G18_001840"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="R8G18_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HEE9795558.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="R8G18_001841"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="R8G18_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HEE9795559.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="R8G18_001842"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="R8G18_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HEE9795560.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="R8G18_001843"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="R8G18_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HEE9795561.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="R8G18_001844"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="R8G18_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HEE9795562.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="R8G18_001845"
     CDS             complement(10994..11137)
                     /locus_tag="R8G18_001845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795563.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="R8G18_001846"
     CDS             11138..11416
                     /locus_tag="R8G18_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795564.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="R8G18_001847"
     CDS             complement(11463..13559)
                     /locus_tag="R8G18_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HEE9795565.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="R8G18_001848"
     CDS             complement(13559..14296)
                     /locus_tag="R8G18_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HEE9795566.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="R8G18_001849"
     CDS             complement(14293..14931)
                     /locus_tag="R8G18_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HEE9795567.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="R8G18_001850"
     CDS             complement(15045..15287)
                     /locus_tag="R8G18_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795568.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="R8G18_001851"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="R8G18_001851"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEE9795569.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="R8G18_001852"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="R8G18_001852"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HEE9795570.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="R8G18_001853"
     CDS             19524..19796
                     /locus_tag="R8G18_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HEE9795571.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="R8G18_001854"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="R8G18_001854"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HEE9795572.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="R8G18_001855"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="R8G18_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="R8G18_001856"
     CDS             22056..22862
                     /locus_tag="R8G18_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEE9795573.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="R8G18_001857"
     CDS             22872..23279
                     /locus_tag="R8G18_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HEE9795574.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="R8G18_001858"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="R8G18_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="R8G18_001859"
     CDS             23839..24636
                     /locus_tag="R8G18_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEE9795575.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="R8G18_001860"
     CDS             24647..25471
                     /locus_tag="R8G18_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEE9795576.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="R8G18_001861"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="R8G18_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="R8G18_001862"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="R8G18_001862"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HEE9795577.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="R8G18_001863"
     CDS             complement(27914..29545)
                     /locus_tag="R8G18_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEE9795578.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="R8G18_001864"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="R8G18_001864"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEE9795579.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="R8G18_001865"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="R8G18_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HEE9795580.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..35777
                     /gene="espL4"
                     /locus_tag="R8G18_001866"
                     /pseudo
     CDS             34789..35777
                     /gene="espL4"
                     /locus_tag="R8G18_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8595 a         9228 c         9105 g         8849 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgttttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc aggtccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtactg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtcag tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctgcgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg ggatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccgcca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa aacacgacac tcactcaaac cattgctcca atatttgaag
    35101 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
    35161 taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt caatgttatt
    35221 ttccggggcg accccaaaac cgtatagtat gccgacacat tgctgcacaa tatattaatg
    35281 atatttatca gaatgttgat tacaaacccc atcaagatga ttattcttca gctgaaaaat
    35341 ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt tccagccgtc
    35401 ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct agttatgaaa gcatattttg
    35461 acaagatgga atcaaatggc atcagtgtta tggcagccat attactggtg gataaccatg
    35521 ctttgacggt ccggctaaga ataaagaaca caactgaagg atgtacccat tacgtggttt
    35581 cggtttatga tcctaatgta actaacgaca aaataagaat catgagcgaa agcaaagagg
    35641 atattaaaca ctattctctg atggatttta tgaatgtaga ttatagcctc ctgaaatggt
    35701 caaatgatca tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc
    35761 tattgatatt aaaagga
//
LOCUS       DAQSWQ010000037        34981 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.037, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000037 DAQSWQ010000000
VERSION     DAQSWQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.037"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..815)
                     /locus_tag="R8G18_001867"
     CDS             complement(<1..815)
                     /locus_tag="R8G18_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HEE9795581.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="R8G18_001868"
                     /pseudo
     CDS             907..993
                     /locus_tag="R8G18_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="R8G18_001869"
     CDS             complement(1397..1873)
                     /locus_tag="R8G18_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HEE9795582.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="R8G18_001870"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="R8G18_001870"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEE9795583.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="R8G18_001871"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="R8G18_001871"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEE9795584.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="R8G18_001872"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="R8G18_001872"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEE9795585.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="R8G18_001873"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="R8G18_001873"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEE9795586.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6902
                     /gene="bioD"
                     /locus_tag="R8G18_001874"
     CDS             6234..6902
                     /gene="bioD"
                     /locus_tag="R8G18_001874"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEE9795587.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="R8G18_001875"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="R8G18_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEE9795588.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="R8G18_001876"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="R8G18_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEE9795589.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="R8G18_001877"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="R8G18_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEE9795590.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="R8G18_001878"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="R8G18_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEE9795591.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="R8G18_001879"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="R8G18_001879"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEE9795592.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="R8G18_001880"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="R8G18_001880"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HEE9795593.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="R8G18_001881"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="R8G18_001881"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEE9795594.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13839..14543
                     /locus_tag="R8G18_001882"
     CDS             13839..14543
                     /locus_tag="R8G18_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEE9795595.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14748..15461
                     /locus_tag="R8G18_001883"
                     /pseudo
     CDS             14748..15461
                     /locus_tag="R8G18_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15526..16237)
                     /locus_tag="R8G18_001884"
                     /pseudo
     CDS             complement(15526..16237)
                     /locus_tag="R8G18_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="R8G18_001885"
     CDS             complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="R8G18_001885"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEE9795596.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17475..18236)
                     /locus_tag="R8G18_001886"
     CDS             complement(17475..18236)
                     /locus_tag="R8G18_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEE9795597.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18369..18779
                     /locus_tag="R8G18_001887"
     CDS             18369..18779
                     /locus_tag="R8G18_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HEE9795598.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18741..19847)
                     /locus_tag="R8G18_001888"
     CDS             complement(18741..19847)
                     /locus_tag="R8G18_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9795599.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19858..21000)
                     /locus_tag="R8G18_001889"
     CDS             complement(19858..21000)
                     /locus_tag="R8G18_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9795600.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="R8G18_001890"
     CDS             complement(20993..22729)
                     /locus_tag="R8G18_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEE9795601.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="R8G18_001891"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="R8G18_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HEE9795602.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="R8G18_001892"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="R8G18_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HEE9795603.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="R8G18_001893"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="R8G18_001893"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HEE9795604.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="R8G18_001894"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="R8G18_001894"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HEE9795605.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="R8G18_001895"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="R8G18_001895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEE9795606.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="R8G18_001896"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="R8G18_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HEE9795607.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="R8G18_001897"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="R8G18_001897"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HEE9795608.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="R8G18_001898"
     CDS             complement(31413..31673)
                     /locus_tag="R8G18_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEE9795609.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="R8G18_001899"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="R8G18_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HEE9795610.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="R8G18_001900"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="R8G18_001900"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HEE9795611.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="R8G18_001901"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="R8G18_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8270 a         8976 c         9490 g         8245 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggattt gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggccggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctcag ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 tttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tatccgccac
     7861 caaagcgatg ctggagcggc gtgatgtggt tgtagtggcc tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat gaacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtggt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtactg gtcaccacac tgaccaagcg gatggcggaa gatcttaccg aatatctcga
     8881 agaacatggc gagcgcgtgc gttatcttca ttcagatatc gacaccgtcg aacgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtgctg gtagggatca acttactgcg
     9001 cgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gactattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataagat caccccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggaa ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gtcgcctaaa gcgttgcaac agaaaatcca tgagctggaa gggttaatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agctgcgtga
     9481 gctgtttatc gcggcttcgt gacaggataa cgaagaagac agatgacaaa agcaaaattg
     9541 cctgatgtgc tacgcttatc aggcctacat gatctctgca atacattgaa ttagcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttt cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctgaatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataga ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattcg gcgttaataa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcgacggg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgct
    10381 cggttccgtt atcagttggt tgaggcagtt gcgcatatcg ccccaggcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tacctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaactgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtctgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga tcgatgctgc atttgaggtc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagtga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aagacgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatttgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgataaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtgtt gattacgggg
    12241 gggactggcc tgacggaagg tgatcaggct cccgaagcat tgctgccgtt gttcgaccgt
    12301 gaagttgaag gttttggtga agtgttccgg atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gccaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgtcggt attcaggcgg
    12721 caaaacgcac ctgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggctga
    13321 gcgtgacgaa gacggtgcag tggtcacgtt taccggtaag gtgcgcaacc ataacctggg
    13381 tgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaatcgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatca
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg cgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atattttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatataaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgctataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgagcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgtgtt tttttcatta gccgtaaatg cacatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagccca gtacaaagaa
    15541 atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac
    15601 gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc
    15661 tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat
    15721 catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg
    15781 caactccacc acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt
    15841 ggtggtaata ctgagcgaga aaatccgcgt aatagtgcta ccgggcaacc ccagacgaga
    15901 gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca ggttgaaggc
    15961 gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa
    16021 gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca gcgaccgcac
    16081 taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt
    16141 ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct
    16201 tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc
    16261 agtttctacc cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag
    16321 acgtggcgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa
    16381 cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg tttcatccac
    16441 ctgctgacaa tctgcggcga taatgccgtt cagattatcg cgcagcgtct ggttaaaatt
    16501 acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt
    16561 actggaccct actgtcgccc agtgatcgtc catcaatgct actttgccgt ggagcgggcg
    16621 gcggcggtac tcaaacacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc
    16681 gccgactctg acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc
    16741 gcggcgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt
    16801 ggcgataatg acttcccgcc gcgcctgagt gagcattttc agatagtggc gttctatatc
    16861 atcgcggtgt tcttcgttat cgcgccagac cagcaatacc tgcgcgtctc ccggctggcg
    16921 gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag
    16981 gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta
    17041 atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc
    17101 aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt
    17161 ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta cgccagctgc
    17221 cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac
    17281 ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac
    17341 gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc
    17401 cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc
    17461 gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt
    17521 cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc
    17581 ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg
    17641 cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc
    17701 cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat
    17761 tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac
    17821 atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc
    17881 ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac
    17941 agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg
    18001 ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc
    18061 agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg
    18121 gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct
    18181 ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt
    18241 ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg
    18301 tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga
    18361 gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga
    18421 ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg
    18481 ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt
    18541 ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca
    18601 cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gtactggaaa
    18661 acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg gtagtgtctt
    18721 tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac
    18781 cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc
    18841 gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac
    18901 gtcaggtttt gcagccatac tggcatgttt tccaccggag aaacgtaacc ggaaaggaga
    18961 atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt
    19021 gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag
    19081 aacagcgcca gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca
    19141 atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc
    19201 caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg
    19261 acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc
    19321 accacaaacc atttgtagtc aagattcggg ttataccagt tgcgtaccac cagctcgctg
    19381 ttgttaggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc
    19441 aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac
    19501 ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt
    19561 aacgcctttt gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca
    19621 tgggtaaagg cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta
    19681 tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac
    19741 aggatcacct gaataagcac aggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc
    19801 aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta
    19861 atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat
    19921 caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg
    19981 cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg
    20041 catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagga acgcggcgtt
    20101 cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat
    20161 ccccagggta ctgagtaaaa acaggctgga gataaaaaac agaataagca gcgaaccgcg
    20221 atacggcacg ccgagaataa acactgacac cagcatacac agcagcatcg ccagcatccc
    20281 gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga
    20341 gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat
    20401 cgcgccgatg accgtcatga tgatggtcac tgcaccgggg ataatgaagt gctggctaat
    20461 cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc
    20521 gttgtcctcc gctcgctgca tttgccagat ctgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgcg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tatgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag tcggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAQSWQ010000038        34771 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.038, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000038 DAQSWQ010000000
VERSION     DAQSWQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.038"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(209..3313)
                     /gene="acrF"
                     /locus_tag="R8G18_001902"
                     /pseudo
     CDS             complement(209..3313)
                     /gene="acrF"
                     /locus_tag="R8G18_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="R8G18_001903"
     CDS             complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="R8G18_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HEE9795612.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4881..5543
                     /gene="acrS"
                     /locus_tag="R8G18_001904"
     CDS             4881..5543
                     /gene="acrS"
                     /locus_tag="R8G18_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HEE9795613.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(5546..5725)
                     /locus_tag="R8G18_001905"
     CDS             complement(5546..5725)
                     /locus_tag="R8G18_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HEE9795614.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5809..6693)
                     /gene="yhdJ"
                     /locus_tag="R8G18_001906"
     CDS             complement(5809..6693)
                     /gene="yhdJ"
                     /locus_tag="R8G18_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HEE9795615.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6779..7075)
                     /gene="fis"
                     /locus_tag="R8G18_001907"
     CDS             complement(6779..7075)
                     /gene="fis"
                     /locus_tag="R8G18_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HEE9795616.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(7101..8066)
                     /gene="dusB"
                     /locus_tag="R8G18_001908"
     CDS             complement(7101..8066)
                     /gene="dusB"
                     /locus_tag="R8G18_001908"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEE9795617.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(8395..9276)
                     /gene="prmA"
                     /locus_tag="R8G18_001909"
     CDS             complement(8395..9276)
                     /gene="prmA"
                     /locus_tag="R8G18_001909"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEE9795618.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(9288..10739)
                     /gene="panF"
                     /locus_tag="R8G18_001910"
     CDS             complement(9288..10739)
                     /gene="panF"
                     /locus_tag="R8G18_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HEE9795619.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10729..10971)
                     /locus_tag="R8G18_001911"
     CDS             complement(10729..10971)
                     /locus_tag="R8G18_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HEE9795620.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(11080..12429)
                     /gene="accC"
                     /locus_tag="R8G18_001912"
     CDS             complement(11080..12429)
                     /gene="accC"
                     /locus_tag="R8G18_001912"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEE9795621.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(12440..12910)
                     /gene="accB"
                     /locus_tag="R8G18_001913"
                     /gene_synonym="fabE"
     CDS             complement(12440..12910)
                     /gene="accB"
                     /locus_tag="R8G18_001913"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEE9795622.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13888..14862)
                     /locus_tag="R8G18_001914"
     CDS             complement(13888..14862)
                     /locus_tag="R8G18_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEE9795623.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15014..16954
                     /gene="csrD"
                     /locus_tag="R8G18_001915"
     CDS             15014..16954
                     /gene="csrD"
                     /locus_tag="R8G18_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HEE9795624.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17259..18302
                     /gene="mreB"
                     /locus_tag="R8G18_001916"
     CDS             17259..18302
                     /gene="mreB"
                     /locus_tag="R8G18_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HEE9795625.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            18368..19471
                     /gene="mreC"
                     /locus_tag="R8G18_001917"
     CDS             18368..19471
                     /gene="mreC"
                     /locus_tag="R8G18_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEE9795626.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            19471..19959
                     /gene="mreD"
                     /locus_tag="R8G18_001918"
     CDS             19471..19959
                     /gene="mreD"
                     /locus_tag="R8G18_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEE9795627.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19968..20561
                     /gene="yhdE"
                     /locus_tag="R8G18_001919"
     CDS             19968..20561
                     /gene="yhdE"
                     /locus_tag="R8G18_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HEE9795628.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            20551..22020
                     /gene="rng"
                     /locus_tag="R8G18_001920"
     CDS             20551..22020
                     /gene="rng"
                     /locus_tag="R8G18_001920"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEE9795629.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            22088..25888
                     /gene="yhdP"
                     /locus_tag="R8G18_001921"
     CDS             22088..25888
                     /gene="yhdP"
                     /locus_tag="R8G18_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HEE9795630.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            26044..27489
                     /gene="tldD"
                     /locus_tag="R8G18_001922"
     CDS             26044..27489
                     /gene="tldD"
                     /locus_tag="R8G18_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEE9795631.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(27617..28546)
                     /gene="aaeR"
                     /locus_tag="R8G18_001923"
     CDS             complement(27617..28546)
                     /gene="aaeR"
                     /locus_tag="R8G18_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HEE9795632.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            28729..28932
                     /locus_tag="R8G18_001924"
     CDS             28729..28932
                     /locus_tag="R8G18_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HEE9795633.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28940..29872
                     /gene="aaeA"
                     /locus_tag="R8G18_001925"
     CDS             28940..29872
                     /gene="aaeA"
                     /locus_tag="R8G18_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HEE9795634.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29878..31845
                     /gene="aaeB"
                     /locus_tag="R8G18_001926"
     CDS             29878..31845
                     /gene="aaeB"
                     /locus_tag="R8G18_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HEE9795635.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31937..32209
                     /locus_tag="R8G18_001927"
     CDS             31937..32209
                     /locus_tag="R8G18_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEE9795636.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(32265..32528)
                     /gene="yhcN"
                     /locus_tag="R8G18_001928"
     CDS             complement(32265..32528)
                     /gene="yhcN"
                     /locus_tag="R8G18_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HEE9795637.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32893..33363)
                     /gene="argR"
                     /locus_tag="R8G18_001929"
     CDS             complement(32893..33363)
                     /gene="argR"
                     /locus_tag="R8G18_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HEE9795638.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33798..34736
                     /gene="mdh"
                     /locus_tag="R8G18_001930"
     CDS             33798..34736
                     /gene="mdh"
                     /locus_tag="R8G18_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEE9795639.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8493 a         8636 c         9075 g         8567 t
ORIGIN      
        1 tcttattaac gagctgaatg ccattttatc accggaggtt ataaatattg cggaatgaat
       61 aatgacaaat tatgtgaatt gtaaaaatag tgaagggagc gggaactata gaaagttttt
      121 aaagagtgag tagaagaata tattattaat ttttatgggt ttcactgaaa ataataaagg
      181 cacccgaaag tgcctttatg tttctgattt atcctttaaa gcaacggcgg atcaccacaa
      241 agaacaccgg tacgaagaag attgccagca acgttgcaga gaccattcct cccattaccc
      301 cgatacccac tgcgttctgc gcgccactgc cggcaccgtt actgatagct agcggtaata
      361 cgccgagaat aaaggcgaga gaggtcatca ggataggacg cagacgcata cgtactgcca
      421 tcagtgtcgc ttcaacaaca cctttaccct ttttctccat gagatcttta gcgaactcaa
      481 caatcaaaat agcgtttttg gccgacaagc caattgtcgt tagcaagccc accataaagt
      541 agacgtcatt tttttgatta aagagtgttg ccgccagtag cacgccgaca atccctaacg
      601 gcacgaccaa cataaccgag acaggaattg accagctttc atagagtgca gcaaggcaca
      661 ggaagacaac cacaaaggaa attgctacca gagcgggagc ctggtttccc gataagcgtt
      721 cctgatacga catacctgtc cagtcataac caatgcccgc aggtaatttt gacgcaaggt
      781 tttccatcaa tgccatggca tcgccggaac tggttcctgg cgcggcttcc ccctgaatct
      841 ccattgacgg cagaccgttg tagcgttcca gtcgcggaga gccatacacc caatgtgaag
      901 tggtaaaggc agagaatggc accatttcgc cgttggcgct gcggacataa agtttatcga
      961 catcttctgg cagcatacgg aatttggcat ccgcctgaac atacaccttt ttcacgcgac
     1021 cacggtcgat gaagtcgtta acgtaagtcc cacccagcgc cgttgaaatg gtctgattga
     1081 tgtcagaaag tgaaacacct aatgcctgcg ccttttcctg gtcaacttcc agtttaaact
     1141 gcgcggtgtc ttccagacca ttaggacgca cgctgactaa gctggcagga tgttgcgccg
     1201 ccatgccaag caactggtta cgggcctggg tcagggcatc gtgacccagc ccagcctgat
     1261 caattaactc aaagtcgaaa cccgttgccg tgcccagttc aacaatggct ggcatattga
     1321 atggaatgac aaaaccgtcg cggatcttgc ccaattccat tttggcacga tggattaccg
     1381 cttccgcact gttttcgtca ccactacgct cttcccacgg tttcagactg acgaaagcca
     1441 taccggcgtt ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag acactttcta
     1501 cgttcgcttt ctcgttattc agatagtaat ccgtcacttg atccaacact ttttgcgtcc
     1561 gctcttgcgt cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc tgatcctctt
     1621 caggtaagaa ggaagacgga agacgtaaaa acaacaccac cattcctgca acaatcagcg
     1681 catagatcag taaatatcgt cctgtggaac cgaggatttt gccgacgctg ttggtgtagt
     1741 ggttaacgct atgatcgaag gtggtattaa accagccgaa gaaaccgccc ttattttcgt
     1801 gatgctcagc agagacgggt ttaagcagcg ttgcacataa cgcaggggta agaatcaatg
     1861 ccaccagaac agaaagcgcc attgccgaaa cgatggtgat agagaactgg cgataaattg
     1921 ccccagtaga accgccgaag aatgccatcg gaataaatac cgctgacagc accatcgcga
     1981 tacccaccag tgcgccctga atttgcgaca tcgatttttc cgtcgcttct tttggcggga
     2041 gcttatcctc catcatcacg cgctcgacgt tctccaccac cactatcgca tcatcgacga
     2101 gcagcccgat ggcaagcacc atcccgaaca tcgttagtgt gttgatggag taaccaaaag
     2161 cggcgaggat ggcaaacgtc cctaacaaca ccacgggtac cgcaatggtg gggatcagcg
     2221 ttgctcgcat attctgcaag aacagataca tcaccaggaa caccagcata atggcttcga
     2281 acagcgtttt taccacttcg tgaatagaaa gctggacgaa tggcgtggtg tcataagggt
     2341 agagaacctt cattccctgc gggaagaatg gctgtaattc cgccagtttt gccttaatgg
     2401 ctttcgcggt atcgagagcg ttcgcgccgg ttgctagctt aatccccagg cccgccgccg
     2461 gttttccgtt gatacgagcg ataacgttat agttttcacc gccaagttca actcgtgcga
     2521 catctttcag gcgtaccacc gagccgtcac tgtttacgcg cagggttact ttgccgaatt
     2581 cttccggatt ttttaaccgc gtctgagcaa taatcgaggc gttcaactgt tgccctggta
     2641 acgctggcgt tccgcccaac tgcccggcag cgatctgatc gttctgtacc ttcaactggt
     2701 taatcacatc aaccggtgtc agtttatatt tgtttagcag atcggcgtcc agccagatac
     2761 gcatcgcata ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga cggctaagcg
     2821 tatctttaac gttagaggcc acatagtccg agatatcgtc ctgtgtggtg tctgggttat
     2881 cagagacaaa gcccgccacc atcaaatagc tgctactgga cttttcaaca ctgatcccct
     2941 gctgctgaac ctcctgcggc agcaacggcg tggcgagctg gagtttgttc tgcacctgca
     3001 cttgcgcgat atcaggatcg gtcccggact ggaaggtaag ggtaattgtc acgctaccgg
     3061 cggaatcgct ggtggaggac atatacatca ggttatcgat accattcata ttctgttcga
     3121 taacctgcgt caccgtatcc tgcacggtct gcgcatcagc acctggatag tttgcctaaa
     3181 cagaaaccgc aggcggtgca atggttggat actgagcgac gggcaattgt aggatcgcca
     3241 gtgcgcccgc catcatcaga ataatggcca gcacccatgc aaatatcggt cgtcgaataa
     3301 aaaagtttgc catgtcagat taccttactt cgatgcagta tctgcggggg tatcggtagt
     3361 ggctttaacc tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga cttgatcgcc
     3421 agatttcaga ccttcgctaa tcaaccattt atcgccaatc gcctgactgg caacgacagg
     3481 gcgcgcttcc acctgacttt tatcgttaac aatcagcacg gttgcatcac cacgcggtgt
     3541 gcggctaacg ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt catcaatccg
     3601 tgcacgcaca aacatacccg gcaaaagcgt atgttgcggg ttagggaaga cagcacgtag
     3661 ggttatggag ccggtgcatt catcaacggt cacatcagag aattgcagcg tacctttcag
     3721 gggataggtt tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt tttccttatg
     3781 caaatttcct tgctctacgg attgcttcag cctcataaaa tcgttgctgg attgggtcac
     3841 atcaacgtag ataggatcga gctgctggac agtcgccagt tcagtcgttt gcccattagt
     3901 gacaagagcg ccttcggtca cggtcgattt gccgatacgt ccgctaattg gcgcagtcac
     3961 tttggtataa gcgagattga tgcgagcgct ttcgactgtg gcttttgcgg caatcacggc
     4021 ggcatcggcc tggcgagcat cagcaatggc ctggtcgtac tcctgctggc tgatgtattt
     4081 cgtacccacg agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg cggcggcttc
     4141 acttttcgcc agttcgcctt tcgcgctgtc ataatttgcc tgataggtcg cgggatcgat
     4201 ctggtacagg gactggcctg cttgtacatc gctgccttca gtgaaattac gattcagtac
     4261 gatcccgcta acctgtgggc gaacttcggc tatacggtaa gcattggtgc ggcctggtaa
     4321 ttcagtctta acttctaacg gggccgtttt cacaatatga acggtaacct gcggttcacc
     4381 gacgtgagct ttctcttctc ccttatcgtt acaaccgacg attaaagccg cggagatcag
     4441 aataaaggag ggcaggagga aaaacctggc atgtttcgtc attactattc ctcaaaaaaa
     4501 caaaagcgcg ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga aaaatacagt
     4561 ttgctatcct acaaattatc attcgtcgat gtaaggaata gttatgaata ctggcgtctc
     4621 aaggcacata aacacaaaaa aagattaata ttctactgtt ttattttgac gcgggttgaa
     4681 agaggcagaa ttaaaacctc gtaaattgaa atatatattc atgtggcgaa tgtattttag
     4741 gtaaataata tatattattt atttgacgat ttatagcaac tcaatgttac cctctcgtat
     4801 aaatacacat tagatgatag attaaccttc gctattttct caccctgtgt cgaatatatt
     4861 tatttcctga ataattaatc atggcaaaaa gaaccaaagc cgaagctctg aagacccggc
     4921 aagaactgat tgaaactgcc atcgcccagt ttgcgcaaca tggcgtaagc aagacgacgt
     4981 tcaacgacat tgccgatgcc gctaacgtta cgcgtggcgc tatctactgg cacttcgaga
     5041 acaagaccca actgtttaat gagatgtggt tgcaacagcc ttcattgcgg gagttaatcc
     5101 aggaacactt gacggctgga ttagagcatg acccgtttca acaattgcgt gaaaaattga
     5161 ttgtcggctt gcaatatatt gccaaaattc cccgccagca ggcgttgctg aaaatcttat
     5221 atcacaaatg tgaatttaat gatgggatgc tggccgaggg agtgatacgc gaaaagatgg
     5281 gctttaatcc gcagactctc cgcgaagtat tgcaggcgtg tcagcaacaa ggttgtgtag
     5341 caaataacct cgatttagat gttgtgatga ttattattga tggtgccttc agcggaattg
     5401 ttcaaaactg gttaatgaat atggcgggtt atgatcttta taaacaagca cccgcgctgg
     5461 tcgataacgt attaagaatg ttcatgccag atgaaaacat aacgaaatta attcatcaaa
     5521 cgaatgaatt aagtgtcatg taaattcaca ttctgtcctg aaaattcagt tcgtcacagt
     5581 taaccagttt tagtggataa actgagttca tattacggca tttgtctgtt tctgttgtca
     5641 gcagttcaat ccactgcatc agtaaagtat caaacagaaa aacgaagaca gcgaaaacga
     5701 cgagccacca atacttgcga atcattacct gagcctgtgc atgtttaacc aagtctggca
     5761 ggaaatatac acgaaatgaa taatctgaaa accaggctta cacttccttt actttgcaat
     5821 gagatcgggg tcaacttcgc ttaaccggct tcgttttgac aggttgccgg tctttctttt
     5881 tttcactttc gccagttctt ccgcggagta atgcgacgcg acatccagcc gtcgaagccc
     5941 cattttgatg tactcgctgt tgatctcaat accaatgaat tttcgtccgc tggcgatggc
     6001 tacggcaccg gtagtaaagc taccggcaaa cgggtcgaga acgatatcgc ctgggttgga
     6061 agaggcgaga ataatgcgtt tcagtaaggc ttccggtttt tgcgtcgggt ggttctcata
     6121 ttcatccatc aaataacgca cgcgcggaaa atcccagacg ttacccggta ctttttgatg
     6181 attgtatggc tgtggaggat ttttgcgata atcgatcaac gcgcgctgcg atccggtttt
     6241 ggcttctacc agaatagcat caccgttgaa tgtgtagttc tttgcgtctt tcaccatcat
     6301 caggatgggt tcgtacatgg agccgtagtg ttttttcgcc tgtactccag aactgtcata
     6361 tgaccagacg atgcgacttt tgatggtaaa aagcttgcgg cactggagat cgataaaggg
     6421 catgttttcc gtactgttca taatgtacat gctgccctgc tttttcagaa cgcggtggca
     6481 ctctacaatc acttcaaaca gccagtcgat aaacagatct tctttccagg cttcgatcag
     6541 accatcaaaa tttttaccga tgttatacgg tgggtcggca aagatcagat cgacgctttc
     6601 agtaggtagc tttttaagtt cggcaagggc atcaccgtga ataatggtct tagcttcatt
     6661 accaaaccgg gtcggttcac atcctgttct catggtcact ccctttgtga cacctataaa
     6721 aaaggcgctt ccccatgccg agtagcgcct ttttaatcaa gcatttagct aacctgaatt
     6781 agttcatgcc gtattttttc aattttttac gcagcgtacc acggttgatg cccatcatca
     6841 gcgcagcacg ggtctggtta ccacgggtgt attgcatcac catgtccaac aggggctgtt
     6901 ctacttcagc cagtaccagc tcatagaggt cattcacatc ctgaccattc agttgagcaa
     6961 aatagttctt cagtgcctgt ttaaccgagt cacgcagggg tttttgggtt acctgatcct
     7021 gagagttaac ggtagaaacg gtcagtacgt cagaatttac gcgttgttcg aacatagttc
     7081 tgtcagctct ttatttctgt ttacgcaaaa ttttcgaagt atgcctccaa cgcctccagc
     7141 tgttcgctgg catcctcaat ggcgttgaat gtgcgccgaa actggtcatt tggagcgtgt
     7201 tcctggagat accaggaaac gtgtttacgt gcaattcggt acccttttgc cggaccataa
     7261 aagtcatgca gttcccgaac gtgcgcgcaa agcaagcgct taacctctgc caaaggcagc
     7321 gggggcagca actccccagt gtccagataa tgctggattt cccgaaagat ccagggtctt
     7381 ccctgagctg cgcggcctat catcagggca tccgcccctg tatagtcgag cacagctctg
     7441 gctttaagcg ggtcagtaat gtcgccattc gcgataaccg gaatggaaac tttctgctta
     7501 actgcccgaa tactgtcgta ctcagcttct ccattgaaca aacaggcgcg tgtacggcca
     7561 tgaatggtca gagcctgaat gccacagtct tcagccagtt gggcaatctc ttcgcagtta
     7621 cggtgttccg gtgcccagcc ggtgcgaatc ttcagggtaa caggaacgtc cactgcattg
     7681 acgacctcgg taaggatcga tttaacgaca tccgggtact gcaagagggc tgaacctgcg
     7741 agcttgcgat tcactttttt agccgggcaa cccatattga tatcaataat ctgggcaccg
     7801 ctttccacgt taatacgtgc tgcatctgcc atttctttcg gatcgctacc agcaatttgc
     7861 acggtgcgaa taccgggttc atcaatgtgc accatccgta aacgagattt gtcgctttcc
     7921 caaacctgtg ggttagaaga catcatctcg gatactgtca atccggctcc catctcgtag
     7981 cacaacgtcc gaaaaggtct gtctgtaatg ccagccatgg gcgctgcgat caggcgattt
     8041 ctgagctgat attgtccgat gcgcatgagt taagaaatga ccatactgtg actgcaaggc
     8101 ggcgtatatt acgcattttt tgcacgagat gaaaggccaa actttgacca atcctctgag
     8161 atggatcaaa gaattgcatt taaaatgagc gtggtgagat aattactcat aaaaatcatc
     8221 atattagaaa atagtgacta aaatttacac tcaaagaaat ttgagtaagt tctcaatttt
     8281 tctttatgaa tgaaaatttt ggcacgcaaa tttgcgtaaa taaccggcaa atttacgtgc
     8341 cttttgtgag cttgctcgca cttcgccccg cgtcacccta cggcgatgcg aaggttaatt
     8401 cttacgaccg gtaatgcggc accactcttc tttttccacg accgggtcca gtgcgaagct
     8461 gtcgacataa gcttcacaaa cgctctctgc ctggcttgcc agaataccgg aaaggcccag
     8521 caaaccgcct gaaaccggca ggacgctgat taacggtgcc agttcacgta atgggcctgc
     8581 aaggatgtta gcgaccacca cgtcggcttt catttcttct ggctgatctt tcggtaagta
     8641 gagttccaga cggtcagaaa cgccattacg ttcggcgttg tcgcggctag cctgaatcgc
     8701 ctgcggatcg atatcaatac caatggcttt tgctgcaccc agtttcagcg ccgcgatagc
     8761 cagaatgccg gaaccacagc caaagtcgat gactatttta ccggttaaat cgaggctgtc
     8821 gagccactgc aggcacagag aggtagttgg atgggtaccc gtaccaaacg ccagccctgg
     8881 atctaacatc acgttgacgg cgttttcgtc cggcacatca cgccagctag ggcagatcca
     8941 cagtcgttca ccaaagcgca tcgggtggaa attatccatc cattcgcgct cccagtcttt
     9001 atcttccagt tgttcgattt tatgcgcgaa gcctgcgccg agcagcggat ggttttccag
     9061 aatcgccacc acgtcgttca tatcggtttc agcgtcgaac agaccaatca catcggtgtc
     9121 gccccacagg cgcgtttcgc ccggcagcgg ctcaaatact ggcgtatcgt gggtatcctg
     9181 aaaagtgata gaaacggcac ccgcttccat cagcgcatcg ctaagatctt cagcgttcgc
     9241 gccggtggtg ttcagtttca gttggatcca aggcatggca aaactcttta tttatcagta
     9301 gtcaaaacgg tagcttgcgg gacggatgta ccgaaacggt ttccgaccag gaaagccagc
     9361 aaacttagta gtaacgaggg cacgataggg tggaagccca ggtactgaat attcagcgtc
     9421 gcgagtacgg catacagcac gccgccaacg atcatcgcac ttagcgcgcc tttggcgttg
     9481 gcgcgttccc agtaaagacc cagcaccagc ggccacagga aaacggcttc cagcccaccg
     9541 aaggccagca aattcagcca gatgatcatt tctggcggct tccaggcggc aagcagcagc
     9601 aacgcgccga gaactaacgt aattaccgcc gacatccgct tcagacgcgt ctcgttttgc
     9661 atttgatccg gacggatatt cagatagaga tctttaatga tcgtagcgga actttgtagc
     9721 aattgggcgt taattgtcga catgatcgca gccataggtg cagccagaaa gatcccggca
     9781 gcaaacggtg gcagcacttt taccattaac gtcgggatca ccagatccgg tacggtgaga
     9841 tcggggatca ctgcacgacc taacgctccg gctaggtgca taccgaacat caggattgcg
     9901 actacaatcg taccgatgat gatcccccgg tgtaccgctt tgctgtcttt ataagagata
     9961 cagcgcaccg cagtatgcgg caggccaatc acgccaaagc acaccagtac ccagaacgac
    10021 gtcataaagg caggcgacag aatatcgtca gcgccttgtg gcgtaaccag ttgcggatcg
    10081 atggtttgca aggtctgtac tgcgttgctt aagccgccag cggcatgtac cacgccaata
    10141 agcagcacaa cggtgccaat cagcatcaca agcccttgca tggtgtcgtt cagcacgctg
    10201 gcgcgaaagc cgccaaaggc ggtatacaac gcaatgctga taccgaaaat cagcagcccg
    10261 gtttcataag gaatacccgc cgcggtttcc agcaggcgcg caccgccgat aaactgcacg
    10321 gtcattgccc caacgaacgc aaccagcaaa ctcaaactcg ccagccacac cagaagacga
    10381 ctctggtagc gggcaaacag catgtcgttc agcgtcactg cattgtagcg gcgcgcaaga
    10441 atcgcaaact tcttgccgag aataccgagt gaaagccaga ctgcaggaag ctgaatcatc
    10501 gccagcaata cccagcccag cccgtattta taagcagctc ctggcccgcc gataaacgaa
    10561 ctggcactga tataggtcgc ggtgagcgtc atcgccagca caataccgcc catagagcgg
    10621 ctgccgagga aatactcatt aaggaaggtg ccggtgctcc gtttacgcat cgcataaacc
    10681 gagataccga acaccaccac cagataggcg accagcggta gaattacttc aagctgcatc
    10741 gtcatcctcc agtgggatat cgcgatagat aaatttcacc atcgcccagc acagtccaat
    10801 aaacagtagc ggcgtcagga tgcaggccat ctcaaaccag cgcggaaagc cggtaaaacc
    10861 gggggcaacg ccaggtaagt aagcggctac taaccaaact gccagataca aaagggtcag
    10921 ccccagcgcc cagcgcgcct ctttatgggc ctgaacaaaa cgagtgtcca ttttttgtcc
    10981 ctgatgggtg aagaaagcga ggattgtacc ttatgggggt tgtcgatccc cagtaataaa
    11041 aaaggccgga aaatccggcc ttttgacgct tttgcagtct tatttttcct gaagaccgag
    11101 ttttttctcc agatagtgga tgttagtgcc accatgctgg aagttctcgt cattcatgat
    11161 gcggatctgc agatcaacgt tggttttgat accgtcgatg atcagctcct gcagcgcatt
    11221 cttcatgcgg gcaatcgcca cgtcacggtt ttcaccgtag caaatcagct taccgatcat
    11281 tgagtcatag tacggcggta cggtgtagcc cgcgtagata tgagactccc aacgtacgcc
    11341 aaaaccgcca ggtgcgtgga aacgggtgat tttgcctggg cttggcagga aggtgttcgg
    11401 atcttcggcg ttgatacgac attccaccgc atggccgcga acgtgaactt cttcttgctt
    11461 gatcgacagc ggttgaccgg cagcgatacg gagctgttct ttgatcaggt caacgccagt
    11521 gatcatttcg gtaaccgggt gttctacctg aatacgggtg ttcatttcga tgaaatagaa
    11581 ctcgccgttt tcgaacagaa actcgaaagt acctgcaccg cgatagccga tatcaacaca
    11641 cgctttagcg caacgttcgc cgatgtagcg acgcagttcc ggggtaatgc ccggtgctgg
    11701 cgcttcttcg accactttct ggtggcggcg ctgcatggag cagtcacgtt ccgccagata
    11761 gatagcgttg ccctgaccgt cagccagtac ctgaatctcg acgtggcgag gattttccag
    11821 gtatttctcc atgtaaacca tatcgttgct gaaagcagct ttcgcttccg cacgggtcat
    11881 ggagatggat tgtgccagtt cagcgtcgcc gcgcactacg cgcataccgc gaccaccgcc
    11941 gccgccggag gctttgataa tcaccggata accaatgcgt ttagcaatgg cacggttttt
    12001 atccatatcg tcgcccaacg ggccgtcaga acccggtacg caagggacgc ccgctttttt
    12061 catcgccgcg attgcggata ctttgtcgcc catcaggcga atggtttctg ctttcgggcc
    12121 gatgaagata aagccggagc gttcaacctg ctcggcaaag ttggcgttct cggagaggaa
    12181 gccgtaaccc ggatggattg ctactgcgcc ggtgatttca gcggcgctga tgattgccgg
    12241 gatgttcaga taacttttta ctgacggagc agggccaata cagaccgttt catctgccag
    12301 taatacgtgt tttagatcgc gatccgcgct ggagtgcaca gcgacagtct tgatgcccag
    12361 ttctttacag gcacgaagaa tacgcaatgc aatctcgccg cggttggcaa taacaatttt
    12421 atccagcatg ttcgcctcgt tactcgatga cgaccagcgg ctcgtcaaat tctaccggtt
    12481 gtccactttc gaccagaatt gctttcacgg taccggattt gtccgcttcg atctggttca
    12541 tcattttcat ggcttcaacg atgcacaggg tatcgcccac gttgactttc tgacccactt
    12601 cgatgaacgc ttttgcgtcc gggcttgggg tgcggtagaa agtaccaacc atcggggaac
    12661 gtacgatgtg accactgatt tccgctgctg ctggcgcttc catggaagga acggtcgccg
    12721 gagcggctgc gttagattga gctggctgct gcatcattgg tgcagcgtaa gcttgttgca
    12781 tcacagggaa acttgcggca ggagctgcac ggctaatgcg tactgactct tcgccttcag
    12841 aaatttccag ttcggagatg cctgattctt caaccagctc gatcagtttt ttaatcttac
    12901 gaatatccat gagtgggttc cgtactcttt gtttagtgtg attgtgacag gcgtttcacc
    12961 gccgtctgta aagcgtatgc cccgacagct gtatgcatag cgataaattc cagcaggccg
    13021 ggacgccggt ctattttgcc ccagagtatc aatgttagac ttgacggaca ttgtgcagcg
    13081 atctgctgct atcctccggc aaaaaacaaa atataccttc gttgcgccaa tgtcaccttt
    13141 tccgcagcgc aaaaactgcg tcagggagga cgaggtcgca cattataacg atttcgtagc
    13201 aattggcagc taaatactgg tctcatcagg gaagataatc aacagctaac atgtaaataa
    13261 ccttcaacac cgtgtaattt gcaacaagcc gcacaattca cgaaattagc gccatcatcg
    13321 acggaacttc ttataacgta aggtgaaaag ctgtaaagcc agccctgcga agatgagggg
    13381 ctgcaatgag ataatcttca cagaccacaa ataatgtagg gatgccagga tcgcgacaag
    13441 atagacgaca ttacgcagcg gttgctgggg attacccaat tttcactgca tcgtctggat
    13501 agatgtgaat gctaaagcac gcaaaatcaa ccgactgaca attctcagca ttaaataggt
    13561 tcgagtggtt aatttgtagt caaataaaat cagattattc accccatggt accagaaatc
    13621 agtaacaggt taagcgtagt gtcgcccagg caaagcacca taaacctaac aggcgacgga
    13681 ggcgtatcaa taatggctgt ttagaggagt atcaatgcgc atcaatttgt ggtcagtcga
    13741 aatcatgact ttccagtttg gatgatgttg tacaacttcc tgaccaaggt gttcgacgaa
    13801 agctgtccgg ctaaccgctt ttcccgttaa ccgattgaat aatgatgtca aaatgagttc
    13861 cctctcttat tattccttgc taaatggtta gttaaccttc accagcgtgc gaccctggat
    13921 ctggttatta atgatggcct cggcaaagtt cggtgcctct gacagagata tctcttttgc
    13981 cgcctgggta tagaatgatt ccggtaaatc ggcgaccagt cgctgccagg cttgtgcgcg
    14041 gcgttctggt ggcgtcatta ctgaatccac cccttgcaaa cggacattac gcagaataaa
    14101 tggcatgacc gtggttggca gagtaaaacc acccgccaga ccacaggccg ccacgcagcc
    14161 gccgtaattc atttgcgcca gcacttttgc cagcactttg tcgccaacag tgtcaattgc
    14221 cccagcccag acttgttttt ccagaggacg ggattcggca aactcatcac gagggagaat
    14281 acggctggca cctaaacttt tcagatattc atgggtactt tcgcgaccgg aaacggcaac
    14341 gacctgataa cccaacttat gcagcagcgc cacggcggta ctgccgacgc caccactggc
    14401 acccgtcacg acaatctccc cgtcctgcgg gcgaacaccg gcatcttcca gcgccatcac
    14461 acacagcatg gcggtaaaac cggcagtacc gataatcatt gctttacgcg cgtccagccc
    14521 ttgcggcatg gcaaccagcc agtcaccttt cactcgcgcc tgctccgcca gcccacccca
    14581 gtggttttca ccaacgcccc agccagtgag taacacctcc tgaccggcat gaaaacgcgg
    14641 atcttcgctg gtgcgtacag ttccggcaaa atcgatccca ggaatcatcg gaaaattacg
    14701 gatgattttt cccttaccgg taatcgccag cgcatcttta tagttcaggc tcgaccagtg
    14761 aacatcgacc gtgacatcgc cctccggcag gcgactttcg tccagagtct gtactgatgc
    14821 gagagttttg ccgtcctgct gttctaaaag taacgcctgc ataagtggtc ctcatgtgca
    14881 tgatggattg gaaaataatt tctgaagatt atactcgcta atggaaaagc aaagccgatg
    14941 aagcgcaata aattgcgaga taaatctgat ttgctagtat gcccgcttcc tcactatcgg
    15001 agttaacaca aggatgagat taacgacgaa attttcggcc tttgttacgc tgctcaccgg
    15061 gttaacaatt tttgtgactt tgctgggctg ttcgctaagt ttctacaacg caattcagta
    15121 taagtttagt catcgcgttc aggcggtggc gacggcgatt gatacccacc ttgtgtcgaa
    15181 tgacttcagc acattaaggc cacaaattac cgaattaatg atgtcggcag atatcgttcg
    15241 tgtagacctg ctccatggtg ataaacaggt ttataccctg gccagaaatg gtagttatcg
    15301 tccggttggc tccagcgatc tgtttcgcga actgagtgtt ccgttgataa agcatccggg
    15361 gatgtcgctg cgtctggttt atcaggatcc gatgggcaac tatttccatt ctttaatgac
    15421 taccgcgccg ctcacggggg cgattggctt tatcattgtt atgctcttcc tggcggtacg
    15481 ctggttacaa cggcaacttg ccgggcaaga attgctggaa acccgggcta ctcgtatctt
    15541 aaacggtgag cgtggctcta atgtgttggg aaccatctat gaatggccgc ccagaaccag
    15601 cagtgcgcta gatacgctgc ttcgtgaaat tcagaacgca cgcgaacaac acagccgtct
    15661 tgatacgctg atccgctctt atgccgccca ggacgtgaaa accggcctca ataaccgact
    15721 ctttttcgat aatcagttag caacgttact ggaagatcag gagaaagtag gtacccacgg
    15781 gatcgtgatg atgattcgtc tgccggattt caatatgttg agcgatactt gggggcacag
    15841 ccaggttgaa gaacagttct tcactctgac taatctgctg tcgacattta tgatgcgcta
    15901 ccctggcgca ctgctggcgc gttaccaccg cagtgatttt gctgcgctgt taccgcaccg
    15961 gacgttaaaa gaggcagaga gcatcgccgg tcagttaatc aaagccgtcg ataccttgcc
    16021 gaacaataaa atgctcgatc gcgacgatat gatccacatt ggtatctgcg cctggcgtag
    16081 tggtcaggat accgagcagg taatggaaca tgcagagtct gccacgcgta atgcgggatt
    16141 gcagggcggc aatagctggg ctatttacga tgactcgttg cctgaaaaag gacgcggtaa
    16201 tgttcgctgg cgtacgctta tcgagcaaat gctcagtcgc ggcggcccgc gcctttatca
    16261 aaaaccggcg gttactcgcg aaggtcaggt tcatcatcgc gaactcatgt gccgcatctt
    16321 cgatggtaat gaagaggtta gctcggcgga gtatatgccg atggtcttgc agtttggctt
    16381 atcggaagag tatgaccgtc tgcaaatcag ccgtcttatt ccactattgc gttactggcc
    16441 agaggaaaat ctggcgattc aggttaccgt tgagtcgctg attcgcccgc gttttcagcg
    16501 ttggctgcgc gatacgttaa tgcaatgtga aaaatcacaa cgaaaacgca taattattga
    16561 acttgcagag gccgatgtag gtcaacatat cagtcgttta caacctgtta ttcgtttagt
    16621 gaatgcttta ggggtacggg tagccgtcaa ccaggctggt ttgacgctgg taagcaccag
    16681 ttggatcaaa gaacttaatg ttgagttact caagctccat ccggggctgg tcagaaacat
    16741 tgagaagcga acggagaacc agctgctggt tcaaagcctg gtggaagcct gctccgggac
    16801 cagcacccac gtttacgcca ccggcgtgcg ttcgcgaagc gagtggcaga ccctgattca
    16861 gcgcggtgtt acaggcgggc aaggggattt tttcgcgtcc tcacagccac ttgatactaa
    16921 cgtgaaaaaa tattcacaaa gatactcggt ttaacctgcc gtttaatccg ttttcacgta
    16981 gaataacgcg cgctgcgtct catgggagtg tgcttgtctg ctcgccagat tgttgcagca
    17041 catatgcaga tgaatgacct tacgcggttg caaacaggcg aggaatgctg ctgatgcatt
    17101 aagcctttct ggactcaggc agagatttgt aacaaaggaa acgaactgca ctaattttca
    17161 ccgtagcaga tgatttttgc gccttgtcgc tgctgcgtgt ggttggtaaa gtaagcggat
    17221 tttcttttcc gccccagctt tcaggattat cccttagtat gttgaaaaaa tttcgtggca
    17281 tgttttccaa tgacttgtcc attgacctgg gtactgcgaa taccctcatt tatgtaaaag
    17341 gacaaggcat cgtattgaat gagccttccg tggtggccat tcgtcaggat cgtgccggtt
    17401 caccgaaaag cgtagctgca gtaggtcatg acgcgaagca gatgctgggc cgtacgccgg
    17461 gcaatattgc tgccattcgc ccaatgaaag acggcgttat cgccgacttc ttcgtgactg
    17521 aaaaaatgct ccagcacttc atcaaacaag tgcacagcaa cagctttatg cgtccaagcc
    17581 cgcgcgttct ggtttgtgta ccggttggcg cgacccaggt tgaacgccgc gcaattcgtg
    17641 aatccgcgca gggcgctggt gcccgtgaag tcttcctgat tgaagaaccg atggctgccg
    17701 caattggtgc tggcctgccg gtttctgaag cgaccggttc tatggtggtt gatatcggtg
    17761 gtggtaccac tgaagttgct gttatctcct tgaacggcgt ggtttactcc tcttccgtgc
    17821 gcattggtgg tgatcgtttc gacgaagcta tcatcaacta tgtgcgtcgt aattacggtt
    17881 ctctgatcgg tgaagccacc gcagaacgta tcaagcacga aatcggttcg gcttacccgg
    17941 gcgatgaagt ccgtgaaatc gaagttcgtg gccgtaacct ggcagaaggt gttccacgcg
    18001 gttttaccct gaactccaac gaaatcctcg aagcactgca ggaaccgctg accggtattg
    18061 tgagcgcggt aatggttgca ctggaacagt gcccgccgga actggcttcc gacatctccg
    18121 agcgcggcat ggtgctcacc ggtggtggcg cactgctgcg taaccttgac cgtttgttaa
    18181 tggaagaaac cggcattcca gtagttgttg ctgaagaccc gctgacctgt gtggcgcgcg
    18241 gtggcggcaa agcgctggaa atgatcgaca tgcacggcgg cgacctgttc agcgaagagt
    18301 aatcggatgc aggcagggga aatgtctgtt taccctgcct ggtctgatac gagaatacgc
    18361 ataacttatg aagccaattt ttagccgtgg cccgtcgcta cagattcgcc ttattctggc
    18421 ggtgctggtg gcgctcggca ttattattgc cgacagccgc ctggggacgt tcagtcaaat
    18481 ccgtacttat atggataccg ccgtcagtcc tttctacttt gtttccaatg ctcctcgtga
    18541 attgctggat ggcgtatcgc agacgctggc ctcgcgtgac caactagaac ttgaaaaccg
    18601 ggcgttacgt caggaactgt tgctgaaaaa cagtgaactg ctgatgcttg gacaatacaa
    18661 acaggagaac gcgcgtctgc gcgagctgct gggttccccg ctgcgtcagg atgagcagaa
    18721 aatggtgact caggttatct ccacggttaa cgatccttat agcgatcaag ttgttatcga
    18781 taaaggtagc gttaatggcg tttatgaagg ccagccggtc atcagcgaca aaggtgttgt
    18841 tggtcaggtg gtggccgtcg ctaaactgac cagtcgcgtg ctgctgattt gtgatgcgac
    18901 ccacgcgctg ccaatccagg tgctgcgcaa cgatatccgc gtaattgcag ccggtaacgg
    18961 ttgtacggat gatttgcagc ttgagcatct gccagcgaat acggatattc gtgttggtga
    19021 tgtgctggtg acctccggtc tgggcggtcg tttcccggaa ggctatccgg tcgcggttgt
    19081 ctcttccgta aaactcgata cccagcgcgc ttatactgtg attcaggcgc gtccgactgc
    19141 aggactgcaa cgtttgcgtt atctgctgct gctgtggggc gcggatcgta acggcgctaa
    19201 cccgatgacg ccggaagagg tgcatcgtgt tgctaatgaa cgtctgatgc agatgatgcc
    19261 gcaggtattg ccttcgccag acgcgatggg gccaaagtta cctgaaccgg caacggggat
    19321 cgctcagccg actccgcagc aaccgacgac aggaaatgca gctactgcgc ctgctgcgcc
    19381 cacacagcct gctgctaatc gctctccaca aagggctacg ccgccgcaaa gtggtgctca
    19441 accgcctgcg cgtgcgccgg gagggcaata gtggcgagct atcgtagcca gggacgttgg
    19501 gtaatctggc tctctttcct cattgcgctg ttgctgcaaa tcatgccctg gccggataac
    19561 ctgattgttt tccggccaaa ctgggtgtta ctcatcttgt tgtattggat cctggccttg
    19621 cctcatcgcg taaatgtggg cacaggtttt gtgatgggtg ccatactgga tctgatcagc
    19681 ggctcgacgc ttggcgtacg cgtattggcg atgagcatca ttgcttacct ggtggcgctg
    19741 aaataccagc ttttccgcaa cctcgcatta tggcagcagg cgctggtcgt catgttgctt
    19801 tcgctggtgg tggatattat tgttttctgg gcagagtttt tagtgattaa cgtctctttc
    19861 agaccggaag tgttctggag tagtgtagtc aatggggtgc tctggccgtg gattttcttg
    19921 ctgatgcgta aagtccgtca gcagtttgca gtgcaataaa ggtttctatg acttctctgt
    19981 atttagcttc cggttctccg cgtcgtcagg agttacttgc gcaacttggt gtgacctttg
    20041 aacgtattgt tacgggcatt gaggagcagc gtcagccgca ggagagcgcg cagcagtatg
    20101 ttgtgcgtct ggcgcgcgag aaagcacagg cgggtgtcgc gcaaacggcg caggatctcc
    20161 cggtgctggg tgcggatact atcgttatcc tgaatggaga agtgctggag aaaccgcgcg
    20221 acgcagagca tgcggcgcag atgttgcgca aattatcggg tcagacccat caggtgatga
    20281 cggcagtggc gttggccgac agccagcaca ttctcgattg cctggtggtc accgatgtga
    20341 ctttcagaac gttaacagac gaagacatcg cgggctatgt cgccagcggt gaaccgttag
    20401 ataaagcagg tgcatacggt attcaggggc tgggtggctg ttttgtcagg aagataaatg
    20461 gcagctatca cgccgtagtc ggcttacctc tggttgaaac gtatgaatta ttaagtaatt
    20521 ttaacgcact gcgtgagaaa agggataaac atgacggctg aattgttagt aaacgtaacg
    20581 ccttcggaaa cgcgagtggc gtatattgat ggcggtattc tgcaggaaat tcatattgaa
    20641 cgtgaggcgc gacgcggaat agtaggcaat atctacaagg gtcgtgtaag tcgtgtactt
    20701 ccgggtatgc aggcggcttt tgtagatatt gggctggata aagccgcgtt tcttcatgca
    20761 tccgatatca tgccgcacac cgaatgtgtg gcgggtgaag aacaaaagca attcacggtg
    20821 cgcgacatct cggaactggt tcgtcagggg caagatctga tggtgcaggt ggtgaaagat
    20881 ccgcttggca ctaaaggtgc gcgcctgacc accgatatca cgctgccttc tcgctatctg
    20941 gtctttatgc caggggcttc tcacgttggg gtttcccaac gtattgaaag cgaatcagaa
    21001 cgtgaacgcc tgaaaaaagt ggtcgcagag tattgcgacg agcagggcgg gtttatcatc
    21061 cgtaccgcag cggaaggggt tggcgaggct gaactggcct ccgatgccgc ttatctgaaa
    21121 cgcgtctgga ccaaagttat ggagcgtaaa aagcgcccgc agacccgtta tcagctgtac
    21181 ggcgaactgg cgctggctca gcgtgttctg cgtgatttcg ccgacgccga actggaccgc
    21241 attcgcgttg actcacgcct gacttacgaa gcattacttg agttcacctc ggagtacatt
    21301 cccgagatga ccagcaagct ggagcattac accggacgcc agccgatttt cgatctcttt
    21361 gatgtcgaaa acgaaatcca gcgagcgctg gaacgcaaag tagaactgaa atccggtggt
    21421 tatctcatta tcgaccagac cgaagcgatg accaccgtgg acatcaatac cggagcgttt
    21481 gtcggtcatc gcaatctgga cgacaccatt ttcaatacca atattgaagc gacgcaggct
    21541 atcgctcgcc agttacggtt gcgtaatctg ggcgggatta tcattattga tttcatcgat
    21601 atgaataatg aagatcaccg ccgccgcgtg ctgcactcgc tggagcaggc gttgagcaaa
    21661 gaccgggtga aaaccagcgt taatggtttt tcggcgctgg ggctggtgga gatgacgcgt
    21721 aaacgcaccc gcgaaagcat tgagcacgta ctgtgtaacg aatgcccaac ctgccacggt
    21781 cgcggaacgg tgaaaaccgt ggaaacggta tgctatgaaa tcatgcgcga gattgttcgt
    21841 gtccaccatg cttacgactc cgaccgtttc ctggtctatg cttctccggc agtagccgaa
    21901 gccttgaaag gcgaagagtc acactcgctg gcggaagtgg aaattttcgt tggcaaacag
    21961 gttaaagtac aaattgaacc gctctataac caggagcagt ttgacgtcgt aatgatgtaa
    22021 acagatgctg ggccgccatc cggcaaaggg tttttgagtc acatttttag cagacaagga
    22081 gtgacgggtg aggcgattgc cggggatttt actgcttact ggagccgcgc tcgttgtgat
    22141 cgctgccctg ctggttagcg gcctgcgtat tgctttaccg catcttgacg cctggcgtcc
    22201 ggaaatcctc aacaaaatag aatccgcgac tggcatgccg gtagaagcca gtcagctctc
    22261 agccagctgg cagaattttg gcccgacgct tgaagcacac gacatccgtg cagaactaaa
    22321 agatggcggc gaattttcgg ttaaacgcgt tactctggcg ctggatgtct ggcagagcct
    22381 gttacatatg cgctggcagt ttcgcgacct cactttctgg cagctgcgct ttcgcaccaa
    22441 cactcctatc accagcggtg gtagcgatga cagtctggaa gccagtcaca tcagcgatct
    22501 gtttcttcgt caatttgacc atttcgatct ccgcgacagt gaagtcagtt tcctgacgcc
    22561 atccggtcag cgtgccgagc tggcgatccc acaactcacc tggctgaacg atccacgtcg
    22621 acaccgtgcg gaaggcctgg taagcctctc cagccttacc ggacagcacg gcgtgatgca
    22681 ggtgcgcatg gatttgcgcg atgatgaggg gttgttaagc aatggtcgcg tctggctaca
    22741 ggcggatgac atcgacctga agccgtggct cggtaaatgg atgcaggaca atattgctct
    22801 ggaaacggca cagttctccc ttgaaggctg gatgacgatc gacaaaggcg atgtaaccgg
    22861 cggtgacgtc tggctgaaac agggcggtgc cagctggttg ggcgagaagc aaacgcatac
    22921 gctgtcggtg gataatctga ccgcgcatat tacgcgtgaa aatccgggct ggcagttctc
    22981 tattcccgat acacggatca cgatggacgg caaaccctgg ccgagcggag cattgacgct
    23041 ggcctggata ccggaacagg acgttggcgg caaagacaat aaacgcagtg acgaactccg
    23101 gattcgcgcc agtaatctgg agctggcagg cctggagggc atacgcccgc tggccgcgaa
    23161 actttcacct gcactgggtg atgtttggcg ctccacacaa ccgagcggca agattaacac
    23221 tctggcgctg gatatcccgc ttcaggcggc agacaagacc cgttttcagg catcgtggag
    23281 cgatctggcc tggaagcaat ggaaattatt accgggtgcg gaacacttct ccgggacgct
    23341 ttccggcagc gttgaaaatg gtttgcttac cgcgtcgatg aagcaggcaa agatgcctta
    23401 cgaaacggta ttccgtgcgc cactggaaat cgccgacggc caggcaacta taagctggct
    23461 gaacaatgac aaaggtttcc agctggatgg gcgtaatatt gacgttaaag ccaaagccgt
    23521 ccatgcgcgc ggcggttttc gttacctgca acctgctaac gatgaaccct ggctgggtat
    23581 tctggctggc atcagtaccg atgatggttc acaagcctgg cactatttcc cggaaaactt
    23641 gatgggtaaa gacctggttg attacttaag tggcgcgatt cagggcggtg aagcggataa
    23701 cgcgacgctg gtttatggtg gcaatccgca actcttcccc tataaacaca acgaaggtca
    23761 gtttgaagtg ctggtgccgc tgcgcaacgc gaagtttgcc ttccagccgg actggcctgc
    23821 attaactaac cttgatattg aactggactt tattaacgac ggtttatgga tgaaaaccga
    23881 tggcgttaat ctgggcggcg tgcgcgcgag taatctcacc gcagtgatcc ctgactactc
    23941 gaaagaaaaa ctgctgattg acgctgacat taaaggtccg ggtaaagccg ttggccctta
    24001 ctttgatgag acaccgctga aagattctct gggtgcgacc ctgcaagaac tccagctcga
    24061 cggcgatgtg aatgctcgct tacatcttga tatcccgctg aacggcgagc tggtaaccgc
    24121 gaaaggtgaa gtgacgctgc gtaataacag tctgtttatc aaaccactcg acagcaccct
    24181 gaaaaatttg agcggtaaat tcagctttat caatggcgat ctgcaaagtg aaccactgac
    24241 agcaagctgg tttaatcagc cgttgaacgt ggatttttcc accaaagaag gggcaaaagc
    24301 ctaccaggtt gcggtgaatc tcaacggtaa ctggcaaccg gcgaaaaccg gcgttctgcc
    24361 tgaagcggtg aacgaagcat tgagtggcag cgtggcgtgg gatggtaaag tgggcattga
    24421 tctgccttat catgctggcg cgacctataa cgtagagctg aacggcgatc tgaataatgt
    24481 gagtagtcac ttaccttcac cgttagccaa acctgcgggt gaaccactag cggtaaacgt
    24541 taaggttgat ggcaatctca acagctttga attaaccgga caggctggtg cggataacca
    24601 tttcaatagc cgctggttgc ttggtcaaaa gctgacgctc gatcgtgcta tttgggcggc
    24661 agacagtaaa acgctcccgc cgttgccgga acaaagtggc gttgaactca atatgccgcc
    24721 gatgaatggt gccgagtggc tggccctgtt ccagaaaggc gctgcggaga gtgtcggtgg
    24781 tgcagcgagt ttcccacaat acataacgtt acgtacgcct atgttgtcac tgggaaatca
    24841 gcaatggaat aacctgagta ttgtttcgca accgacggca aatggcaccc tggttgaggc
    24901 gcaagggcgt gaaatcaacg ccacgctagc gatgcgtaat aacgcgccgt ggctggcgaa
    24961 tatcaaatat ctttattaca acccgagcgt ggcgaaaact cgtggtgatt caacgccgtc
    25021 atcacctttc ccgacaacgg agcgcattaa cttccgtggc tggccggacg cacaaatacg
    25081 atgcacagag tgctggttct gggggcaaaa attcggtcgc attgacagtg atatcaccat
    25141 ttctggcgat acgttaacgc tgaccaatgg actgattgat actggtttct cgcggcttac
    25201 tgccgatggt gaatgggtta ataatccggg gaatgaacgt acctcgctga aaggaaaact
    25261 gcgcgggcag aaaattgatg ccgccgcaga attttttggt gtcacgacgc ccatacgcca
    25321 gtcgtcattt aatgtggatt acgatttaca ctggcgcaaa gcaccctggc agccagatga
    25381 agcgacgttg aatggcatca ttcatactca actgggtaaa ggcgaaatta ccgaaatcaa
    25441 taccggacat gccgggcaat tgctgcgctt attgagcgta gatgccctga tgcgtaagct
    25501 gcgttttgat ttcagagaca cttttggcga agggttctat tttgactcca ttcgcagcac
    25561 cgcgtggatt aaagacgggg ttatgcacac cgacgacacg ctggtggatg gcctggaggc
    25621 ggatatcgcc atgaaagggt cggtaaatct ggtacgtcgc gacctgaata tggaagcggt
    25681 tgtcgcacca gagatttctg cgacggtggg cgtggctgcg gcttttgcgg ttaaccccat
    25741 tgttggcgcg gcagtttttg ccgctagtaa agtgctgggg ccgctgtgga gcaaagtctc
    25801 cattttgcgc tatcacattt cgggtccgct ggacgatccg caaatcaacg aagtgttgcg
    25861 ccaaccgcgt aaagaaaaag cgcaatgatt tgacgagggc gcgtaattgc cccaatctca
    25921 tatgataatc gttgccaaag gccaacgagc cagaacataa ccgtaggccg gatagggcgt
    25981 tcatgccgca tccggcagcc gtaaaaaatc ctctactgca gtaactaacg agtagcaaaa
    26041 acgatgagtc tgaacctggt aagtgaacaa ttgctagcgg cgaacagcct gaaacatcag
    26101 gacttgttcg cgatcctcgg tcaactggcc gaacgtcgcc ttgattatgg cgatctctat
    26161 tttcagtcga gctatcacga atcctgggtt ttagaagacc gcattattaa agatggttct
    26221 tacaacatcg atcagggcgt tggcgtgcgt gcaatcagcg gtgaaaaaac cggatttgct
    26281 tacgctgacc aaatcagcct gctggcgctg gaacagagtg cgcaagcggc gcgcaccatc
    26341 gtccgtgata gtggtgatgg caaagtacag acgctgggcg cggtagagca tagcccgttg
    26401 tatacctcgg tagatccgct gcaaagcatg agccgtgaag agaagctgga tatcctgcgt
    26461 cgcgtcgata aggttgctcg cgaagcggac aagcgcgtac aggaagtgac tgccagcctc
    26521 agtggtgtct atgaattaat tctggttgcg gccaccgacg gcacgctagc ggcggatgtc
    26581 cgtccgctgg tgcgtctttc cgtgagcgtt ctggtcgaag aagatggcaa acgcgaacgc
    26641 ggtgccagtg gcggcggcgg tcgttttggt tatgagttct tccttgccga tcttgacggc
    26701 gaagtccgtg cggatgcatg ggcaaaagaa gcagtgcgta tggcgctggt caatctttct
    26761 gccgttgctg caccagcggg caccatgccg gtagtacttg gcgcaggttg gccgggcgtg
    26821 ctgttgcatg aagcggtagg tcacggtctg gaaggcgact tcaaccgccg cggcacttca
    26881 gtatttagtg ggcaggtcgg ggagctggtg gcttcagaac tgtgtaccgt ggttgatgac
    26941 ggcacgatgg tcgatcgccg tggttcggtg gcgattgatg acgaaggtac gccaggccag
    27001 tacaacgtgc tgattgagaa cggcattctg aaaggctaca tgcaggataa actcaacgcg
    27061 cgtttgatgg ggatgacgcc gactggcaac ggtcgccgtg aatcctacgc ccatctgccc
    27121 atgccgcgta tgactaacac ctatatgctg ccgggtaaat cgaccccgca ggaaattatt
    27181 gaatccgttg agtacggtat ctatgcaccg aactttggtg gcggtcaggt ggatatcacc
    27241 tccggcaaat tcgttttctc cacttcagaa gcatatctga ttgaaaacgg taaagtaacg
    27301 aagccggtga aaggcgcaac gttgattggt tccggtatcg aaaccatgca gcagatttcg
    27361 atggttggca acgacctgaa actggataac ggcgtgggtg tctgcggtaa agaagggcaa
    27421 agtttgccgg ttggcgtggg ccagccaacg ctgaaagttg ataacctgac tgttggcggt
    27481 actgcgtaat aatttcatta ttttcagaag gataattaat ctttctacgt gcacgaacgg
    27541 tcccctcgct cctctggggt tagggtgagg ggaacccgtt ggcacaggtt tgtagaacgt
    27601 aacagtacaa tatgaattac ttctctttcc cgcgcccgtg catctcctga aacaatttac
    27661 cgacctcaac aaaataatcc gtcagcgagt tgatcacgac ctgtaccttc agcggcagct
    27721 tatctttttc ggtatataac gcataaaccg ggcgtggatc tgactgataa cgcggcagca
    27781 ggatctccag ctcaccgcga ttgatctcgt tgatcaccca catcagcggc acgtaggcga
    27841 tcccggcacc cgccgtcagc cagcgcacca gcgtcatcgg atcattagtc acaaatcttc
    27901 cttgtgggat caggcgagtc gagatccctt ccggtgcgat cagttcaaat tcattgtcgg
    27961 gccgcacgct gtattcaagc catgaatgac tactcaaatc ggcgggtttt tccggtatgc
    28021 cgtattgtgt gagatagctt ttcgcggcgc acaccaccat cggcatcgcg cccagacggc
    28081 gggaaaacag gctggaatcc tgcaacgcgc cgacgcggat caccacatcc agaccgtcgg
    28141 caatcaggtc gggggctgga attccggtaa ccagattgac gctcaaacct gggtattctt
    28201 tcagcatttt tgctgtcagc ccggcgagaa cattttgtgc catagttgaa gaacagccaa
    28261 tgcgcagcgt cccgatgggg gtgttattga aggcatacag ttgctcatga acatcctgca
    28321 cttcatgaag catccgacgg cagccctggt agtaaattct gccggcttcg gtcaggccaa
    28381 tgctgcgtgt gctacggttt aacagcttta cctgcaactc atcttccagt tttgacaccg
    28441 tctgactgat ggacgaaacg ctcatctgta gctgtctggc ggcggcggta aaagagccaa
    28501 attcaactac tttggcaaac accgacatgc gttttagtcg ttccattatt cactctgact
    28561 taaaagtgat ttagatcaca taatatagat aacagcataa cagttacgct aatatattaa
    28621 atatcaatct acagcaatgt tgctctcgcc cggctttcca tgcccttctc tgcggcgaca
    28681 gatgctgaaa ataataacgc ctgctctctc tttacaacca aggtcaacat gagtctgttt
    28741 cccgttatcg tggtgtttgg actgtccttc ccaccgatat tttttgaatt gcttttatca
    28801 ctggcgattt tctggctggt gcgccgggta cttgtgccaa caggtatcta cgactttgtc
    28861 tggcatccgg cgttgttcaa caccgcgctc tattgctgct tgttttattt gatatcgcga
    28921 ctgttcgttt gaggttgaag tgaaaacact aataagaaaa ttctcccgta cggccatcac
    28981 ggtcgtatta gtcattctag ccttcatcgc aatttttaat gcctgggtct attacaccga
    29041 atccccctgg acgcgtgacg cgcgctttag cgctgacgtc gttgcgatcg cgccggacgt
    29101 ttctggactc attacccagg tgaatgttca tgataaccag ctggtgaaaa aaggacagat
    29161 actgttcacc atcgaccagc cgcgctatca aaaggcgctt gaggaagcgc aagccgatgt
    29221 tgcttattat caggtactgg cacaggagaa acgccaggag gccggacgtc gtaaccgtct
    29281 cggtgtgcag gcgatgtctc gcgaagagat cgaccaggcc aacaacgtac tacaaacggt
    29341 tctgcatcag ttagcgaaag cgcaggcgac ccgcgatctg gcaaaactgg atcttgaacg
    29401 cacggtgatc cgcgcgccag cagatggctg ggtgaccaac ctcaacgtct ataccggtga
    29461 gtttattact cgaggatcaa cggcggttgc gctggtgaaa cagaactcct tctatgtact
    29521 ggcctatatg gaagaaacta agctggaagg ggggcgtccg gggtatcgtg cagagatcac
    29581 gccgcttggc agtaacaaag tgctgaaagg gactgttgat agtgttgccg caggggtcac
    29641 caacgccagc agcacgcgtg acgacaaagg gatggcgact atagactcta atcttgaatg
    29701 ggtgcgtctt gcgcaacgtg ttccggttcg tattcgtctc gacaaccagc aagagaacat
    29761 ctggcctgcg ggcaccactg ctacagtggt ggtcactggc aaacaagatc gcgacgaaag
    29821 ccaggattcg ttcttccgta aaatggccca tcgcctgcgt gagtttggtt aatcacgatg
    29881 ggtattttct ccattgctaa ccaacatatt cgctttgcgg taaaactggc gaccgccatt
    29941 gtactggcgc tgtttgttgg ctttcacttc cagctggaaa cgccacgctg ggcggtactg
    30001 acagcggcga ttgttgccgc cggtccggcc tttgctgcgg gaggtgaacc gtattctggc
    30061 gctattcgct atcgtggctt tttgcgcatc atcggcacat ttattggctg tattgccgga
    30121 ctggtgatca tcattgcgat gatccgcgca ccattattga tgattctggt gtgctgtatc
    30181 tgggccggtt tttgtacctg gatatcctcg ctggtacgaa tagaaaactc gtacgcgtgg
    30241 gggctggccg gttataccgc gctgatcatt gtgatcacca ttcagccgga accattgctt
    30301 acgccgcagt ttgccgtcga acgttgtagc gagatcgtta tcggtattgt gtgtgcgatt
    30361 atggcggatt tgctcttttc tccgcgatcg atcaaacaag aagtagatcg agagctggaa
    30421 agtttgctgg tcgcgcaata tcaattaatg caactctgta tcaagcatgg cgatggtgaa
    30481 gttgtcgata aagcctgggg cgacctggtg cgacgcacca cggcactaca aggcatgcgc
    30541 agcaacctga atatggaatc ttcccgctgg gcgcgggcca atcgacgttt aaaagcgatc
    30601 aatacgctat cgctgacgct gattacccaa tcctgcgaaa cttatcttat tcagaatacg
    30661 cgcccggaat tgatcactga tactttccgc gaattttttg acacgccggt agaaaccgcg
    30721 caggacgtcc acaagcagct caaacgcctg cggagagtta tcgcctggac cggggaacgg
    30781 gaaacgcctg tcaccattta tagctgggtc gcggcggcaa cgcgttatca gcttctcaag
    30841 cgcggcgtta tcagtaacac aaaaatcaac gccaccgaag aagagatcct gcaaggcgaa
    30901 ccggaagtaa aagtagagtc agccgaacgt catcatgcaa tggttaactt ctggcgaacc
    30961 acactttcct gcattctggg cacgcttttc tggctgtgga cgggctggac ttccggcagt
    31021 ggtgcaatgg tgatgattgc ggtcgtgacg tcactggcaa tgcgtttgcc gaatccacgc
    31081 atggtggcga tcgactttat ctatgggacg ctggccgcgc tgccgttagg gctgctctac
    31141 tttttggtga ttatccctaa tacccaacag agcatgttgc tgctgtgtat tagcctggca
    31201 gtgctgggat tcttcctcgg tatagaagta cagaaacggc gactgggctc gatgggggca
    31261 ctggccagca ccataaatat tatcgtgctg gataacccga tgactttcca tttcagtcag
    31321 tttctcgaca gcgcattagg gcaaatcgtc ggctgtgtgc tcgcgttcac cgttattttg
    31381 ctggtgcggg ataaatcgcg cgacaggact ggacgtgtac tgcttaatca gtttgttttt
    31441 gccgctgttt ccgcgatgac taccaatgtg gcacgtcgta aagagaacca cctcccggca
    31501 ctttatcagc agctgttttt gctgatgaat aagttcccag gggatttgcc gaaatttcgc
    31561 ctggcgctga cgatgattat cgcgcaccag cgcctgcgtg atgcgccgat cccggttaac
    31621 gaggatttat cggcgtttca ccgacaaatg cgccgcacag cagaccatgt gatatctgcc
    31681 cgtagcgatg ataaacgtcg tcggtacttt ggccagttgc tggaagaact ggaaatctac
    31741 caggaaaagc tacgcatctg gcaagcgcca ccgcaggtga cggaaccggt tcatcggctg
    31801 gcggggatgc tccataagta tcaacatgcg ttgaccgata gttaagtcaa aaccgacgcc
    31861 aaaagcgtcg gttttttcat ggctatactt agcgatgcac ggcagaactc gccgcgaaac
    31921 gtgacggtgg caacagatga atatttatac ctttgatttt gatgagattg agagtcagga
    31981 ggatttttat cgtgacttta gccaaacctt tggtctggct aaagataagg tacgcgatct
    32041 cgactcacta tgggatgtgt taatgaacga tgtcctgccg ctaccacttg agattgaatt
    32101 tgttcatctg ggagagaaaa cgcgtcgccg ttttggcgcg ttaatattgc tgtttgatga
    32161 ggcagaggaa gagctggaag ggcatttgcg ttttaatgtt cgtcattagc gcaaaaaaaa
    32221 gcccccgaac cgggggcaat atcgtcggac aagacgatga gggtttattt gtacagttca
    32281 gccgtagcgt gccaggtgtc accgctacga gcttcagtaa tctggtaggc cgttgcgcct
    32341 ttctcttccg cttttttgtt cagcatttca cgcatatcca ttggcgaaga cgccacacca
    32401 cttacggata cggtcccgat tgcttcacga ttttgtgctt gtgcagtatc aatggagtcg
    32461 gcagcgaatg caccgaaaga gagaacagaa agtacgctta atgcagctac agtggttttg
    32521 attttcatga tttttacctc gacataatct tttagctggg cctttgtttc gtgactcata
    32581 tcacaaaatc aagcatacac taattattga actaattaat accacgctaa ctgtttcagt
    32641 tgatactaag tgttaaaaat gtgaatttta ataacaaaaa agaattaaaa acacggttat
    32701 ttattgttaa ttctagtatt ttcaattaga taatgaattt tacataatgt gcttttttta
    32761 ccgtatgtat tcattgtgtg aatgatatgt cgcggtaaaa cgcactattc gttaaattaa
    32821 tcgcagggga aagcaggggg ttgagaggaa taagcaacat tttccccgcc gccagaagcg
    32881 acggggcaga gattaaagct cctggtcgaa cagctctaaa atcgcttcgt acaggtcttt
    32941 gacggtgaaa ccgttagcag gggtggtaaa gatggtgtca tcgccagcga tggtgcccag
    33001 aataccttct gctttgccca gtgagtccag caggcgagca attaactgcg ccgcgccagg
    33061 gctggtatga atcacgacaa ctgcatcgtt gtagtcgata tccagcacca gattcttcaa
    33121 tggactggag gtggttggta cacccagttc agctggcagg cagtaaacca tttccatttt
    33181 ggcattgcgt gtacgtacag caccaaactt ggtcaacatc cgcgagactt tagactgatt
    33241 aatattgtca aagccttgct cctgcaacgc ggcgacgatt tcgccctggg agctaaattt
    33301 ctcttcttta agtaatgctt taaatgcttt aactagttct tcttgcttag ccgagcttcg
    33361 cataagtcac ccgatatggt ggttgataca acattattgt gcatacagat gaatttttat
    33421 gcaaacagtc agccctgaag aaggctgaaa taatgttatg aaagagcggg attttatcaa
    33481 atttcgttat tgagaaacat gcctgcgtca cggcatgcaa attctgttta aaagtaaatt
    33541 aattgttatc aaattgatgt tgttttggct gaacggtagg gtatattgtc accacctgtt
    33601 ggaatgttgc gctaatgcat aagcgactgt taattacgta agttaggttc ctgattacgg
    33661 caattaaatg cataaacgct aaacttgcgt gactacacat tcttgagatg tggtcattgt
    33721 aaacggcaat tttgtggatt aaggtcgcgg cagcggagca acatatctta gtttatcaat
    33781 ataataagga gtttaggatg aaagtcgcag tcctcggcgc tgctggcggt attggccagg
    33841 cgcttgcact actgttaaaa acccaactgc cttcaggttc agaactctct ctgtatgata
    33901 tcgctccagt gactcccggt gtggctgtcg atctgagcca tatccctact gctgtgaaaa
    33961 tcaaaggttt ttctggtgaa gatgcgactc cggcgctgga aggcgcagat gtcgttctta
    34021 tctctgcagg tgtagcgcgt aaaccgggta tggatcgttc cgacctgttt aacgttaacg
    34081 ccggcatcgt gaaaaacctg gtacagcaag ttgcgaaaac ctgcccgaaa gcgtgcattg
    34141 gtattatcac taacccggtt aacaccacag ttgcgattgc tgctgaagtg ctgaaaaaag
    34201 ccggtgttta tgacaaaaac aaactgttcg gcgttaccac gctggacatc attcgttcca
    34261 acacttttgt tgcggaactg aaaggcaaac agccaggcga agttgaagtg ccggttattg
    34321 gcggtcactc tggtgttacc attctgccgc tgctgtcaca ggttcctggc gttagtttta
    34381 ccgagcagga agtggctgat ctgaccaaac gcatccagaa cgcgggtact gaagtggttg
    34441 aagcgaaagc cggtggcggg tctgcaaccc tgtctatggg ccaggcagct gcacgttttg
    34501 gtctgtctct ggttcgtgca ctgcagggcg aacaaggcgt tgtcgaatgt gcctacgttg
    34561 aaggcgatgg tcagtacgcc cgtttcttct ctcaaccgct gctgctgggt aaaaacggcg
    34621 tggaagagcg taaatctatc ggtaccctga gcgcatttga acagaacgcg ctggaaggta
    34681 tgctggatac actcaagaaa gatatcgcct tgggcgaaga gttcgttaat aagtaattga
    34741 ttagcgaata ataaaaaacc ggagcacaga c
//
LOCUS       DAQSWQ010000039        34130 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.039, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000039 DAQSWQ010000000
VERSION     DAQSWQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.039"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            38..580
                     /locus_tag="R8G18_001931"
     CDS             38..580
                     /locus_tag="R8G18_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9795640.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="R8G18_001932"
     CDS             663..1364
                     /locus_tag="R8G18_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEE9795641.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="R8G18_001933"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="R8G18_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="R8G18_001934"
     CDS             3979..5049
                     /locus_tag="R8G18_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9795642.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5075..5602
                     /locus_tag="R8G18_001935"
     CDS             5075..5602
                     /locus_tag="R8G18_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9795643.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            5610..6125
                     /locus_tag="R8G18_001936"
     CDS             5610..6125
                     /locus_tag="R8G18_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEE9795644.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6091..6828
                     /locus_tag="R8G18_001937"
     CDS             6091..6828
                     /locus_tag="R8G18_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HEE9795645.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6939..7949
                     /gene="pyrD"
                     /locus_tag="R8G18_001938"
     CDS             6939..7949
                     /gene="pyrD"
                     /locus_tag="R8G18_001938"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEE9795646.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8123..8665
                     /gene="zapC"
                     /locus_tag="R8G18_001939"
     CDS             8123..8665
                     /gene="zapC"
                     /locus_tag="R8G18_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HEE9795647.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8662..9771)
                     /gene="ycbX"
                     /locus_tag="R8G18_001940"
     CDS             complement(8662..9771)
                     /gene="ycbX"
                     /locus_tag="R8G18_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HEE9795648.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10015..12123
                     /gene="rlmKL"
                     /locus_tag="R8G18_001941"
     CDS             10015..12123
                     /gene="rlmKL"
                     /locus_tag="R8G18_001941"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HEE9795649.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12135..14042
                     /locus_tag="R8G18_001942"
     CDS             12135..14042
                     /locus_tag="R8G18_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEE9795650.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14172..15425
                     /gene="pqiA"
                     /locus_tag="R8G18_001943"
     CDS             14172..15425
                     /gene="pqiA"
                     /locus_tag="R8G18_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HEE9795651.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15430..17070
                     /gene="pqiB"
                     /locus_tag="R8G18_001944"
     CDS             15430..17070
                     /gene="pqiB"
                     /locus_tag="R8G18_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEE9795652.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17067..17630
                     /gene="pqiC"
                     /locus_tag="R8G18_001945"
     CDS             17067..17630
                     /gene="pqiC"
                     /locus_tag="R8G18_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEE9795653.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17886..18053
                     /gene="rmf"
                     /locus_tag="R8G18_001946"
     CDS             17886..18053
                     /gene="rmf"
                     /locus_tag="R8G18_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HEE9795654.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18123..18641)
                     /gene="fabA"
                     /locus_tag="R8G18_001947"
     CDS             complement(18123..18641)
                     /gene="fabA"
                     /locus_tag="R8G18_001947"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HEE9795655.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18710..20470)
                     /locus_tag="R8G18_001948"
     CDS             complement(18710..20470)
                     /locus_tag="R8G18_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HEE9795656.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20656..21108
                     /gene="matP"
                     /locus_tag="R8G18_001949"
     CDS             20656..21108
                     /gene="matP"
                     /locus_tag="R8G18_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HEE9795657.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21184..22224)
                     /gene="ompA"
                     /locus_tag="R8G18_001950"
     CDS             complement(21184..22224)
                     /gene="ompA"
                     /locus_tag="R8G18_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HEE9795658.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22579..23088)
                     /gene="sulA"
                     /locus_tag="R8G18_001951"
     CDS             complement(22579..23088)
                     /gene="sulA"
                     /locus_tag="R8G18_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HEE9795659.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23307..23936
                     /gene="sxy"
                     /locus_tag="R8G18_001952"
     CDS             23307..23936
                     /gene="sxy"
                     /locus_tag="R8G18_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HEE9795660.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23899..26061)
                     /gene="yccS"
                     /locus_tag="R8G18_001953"
     CDS             complement(23899..26061)
                     /gene="yccS"
                     /locus_tag="R8G18_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEE9795661.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26071..26517)
                     /locus_tag="R8G18_001954"
     CDS             complement(26071..26517)
                     /locus_tag="R8G18_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEE9795662.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26640..28694
                     /gene="helD"
                     /locus_tag="R8G18_001955"
     CDS             26640..28694
                     /gene="helD"
                     /locus_tag="R8G18_001955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HEE9795663.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28726..29184)
                     /gene="mgsA"
                     /locus_tag="R8G18_001956"
     CDS             complement(28726..29184)
                     /gene="mgsA"
                     /locus_tag="R8G18_001956"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEE9795664.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29280..29942)
                     /locus_tag="R8G18_001957"
     CDS             complement(29280..29942)
                     /locus_tag="R8G18_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HEE9795665.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30115..30528
                     /locus_tag="R8G18_001958"
     CDS             30115..30528
                     /locus_tag="R8G18_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEE9795666.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30573..30890)
                     /gene="hspQ"
                     /locus_tag="R8G18_001959"
     CDS             complement(30573..30890)
                     /gene="hspQ"
                     /locus_tag="R8G18_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEE9795667.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30948..32138)
                     /gene="rlmI"
                     /locus_tag="R8G18_001960"
                     /pseudo
     CDS             complement(30948..32138)
                     /gene="rlmI"
                     /locus_tag="R8G18_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            32233..32511
                     /gene="yccX"
                     /locus_tag="R8G18_001961"
     CDS             32233..32511
                     /gene="yccX"
                     /locus_tag="R8G18_001961"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEE9795668.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32508..32837)
                     /gene="tusE"
                     /locus_tag="R8G18_001962"
     CDS             complement(32508..32837)
                     /gene="tusE"
                     /locus_tag="R8G18_001962"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HEE9795669.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32928..33587)
                     /gene="yccA"
                     /locus_tag="R8G18_001963"
     CDS             complement(32928..33587)
                     /gene="yccA"
                     /locus_tag="R8G18_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HEE9795670.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33794..33881)
                     /locus_tag="R8G18_001964"
     tRNA            complement(33794..33881)
                     /locus_tag="R8G18_001964"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33845..33847),aa:Ser,seq:tga)
BASE COUNT         8828 a         8129 c         8898 g         8275 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaagatattt cagtgcgtta tcttgttctt
     5101 tagcattctt aacatccatg tcgggatggc tgggcctgaa caagttagta tgcatattta
     5161 tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac aaaatattca
     5221 tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca ctgctgctga
     5281 tttgaatatc gatatcaccg gttgtgccgc tagtattact ggcgcggacg tcctttttag
     5341 cggcgaggct gacaccctta cgccgacact gctcaaacta actgacacag gcggaagcgg
     5401 tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc aagaaatccc
     5461 gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac tcaaatatca
     5521 acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg cggttctcta
     5581 ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg attctgttct
     5641 tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc aacattagcg
     5701 gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc aatgtgcagt
     5761 taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag aaatctttca
     5821 tcattaattt gcaggattgc ggaacagatg tcagcacggt tgacgtcacc ttttcaggaa
     5881 cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat gcagccagcg
     5941 ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac caggcgagta
     6001 aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa ttgcgacctg
     6061 tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta cttcattatg
     6121 actaatacgt ggaacagatt ggcgctcttg attttcgccg ttttatcgct gctggtggcg
     6181 ggtgaattgc gggcaggagt ggtggtcggc ggaacgcgat ttatctttcc ggcagacaga
     6241 gaatcgatat ctattttact gactaatacc agtcaggaat cctggcttat taacagtaaa
     6301 atcaaccgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc accattactg
     6361 gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg cttgctgaga
     6421 acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag cattgccagc
     6481 gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc ggtatttaaa
     6541 ctgttctggc gacccgaagg tttgccgggc gacccgctgg aagcttatca acaattacgc
     6601 tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta cattaacctg
     6661 attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagttgtgcc gcctaaaagc
     6721 cagcgtcaga caagctggtg tcaggcaatc gcaccgtgtc atgtcgcctg gcgggcgata
     6781 aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa caggttcgga
     6841 aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact cgcacataat
     6901 acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc ttcgttcgta
     6961 aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag caattacgcc
     7021 gtattacagg aacgccgttt gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta
     7081 actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt gataaagacg
     7141 gggagtgcat tgacgcgtta ggcgcgatgg gatttggatc gatcgagatc ggtaccgtca
     7201 cgccacgtcc acagccaggt aatgacaagc cgcgtctctt tcgtctggta gatgccgaag
     7261 gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta gagaacgtaa
     7321 aaaaggccca ttatgacggc gtcctgggta ttaacatcgg caaaaataaa gatacgccag
     7381 tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc tatgcgggat
     7441 atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg caatatggtg
     7501 aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg caagcgatgc
     7561 accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa gaagaattga
     7621 tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca accaatacca
     7681 cactcgatcg ttctcttgtt cagggaatga aaaattgcga tcaaaccggt ggcttaagtg
     7741 gtcgtccgct tcagttaaaa agcaccgaaa ttattcgccg cttgtcactg gaattaaacg
     7801 gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg cgtgaaaaga
     7861 ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa ggtccgccgc
     7921 tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt tataagataa
     7981 tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt atttagacat
     8041 tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt tgctgcggtt
     8101 ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta
     8161 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
     8221 ttttgcgcgc aagatgttga ctccagatgc cttttctccc gcaggctttt gcgttgacga
     8281 cgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagaaca
     8341 aaaagccgaa ctggtgctga atgcactggt agcaattcgc tatctcaaac cgcaaatgcc
     8401 gaaaagctgg cattttgttt cccatggtga aatgtgggtg cccatgccgg gcgatgcggc
     8461 ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg aatctggcga
     8521 aaatgccgca ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
     8581 gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtta atgttgacag
     8641 cttcagcctc gaacaggcag tctaacgcgc caacttaagt gcagtcttcg gtacacagct
     8701 acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg tgacttcgcc
     8761 ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg
     8821 aattccctga ttttctaatt gctccagcaa cacctgttgg ttatttccac gaaatgcctg
     8881 tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg cggtatcatc
     8941 agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca cctcatcgcc
     9001 aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
     9061 atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg
     9121 ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa
     9181 caccacatca ccaatgcgaa tcactttcca gcgatcttct tcccaggctg acgcgccgga
     9241 aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac gttgttggag
     9301 atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagagg
     9361 tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac
     9421 ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt
     9481 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
     9541 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt
     9601 aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
     9661 cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg taagaccaat
     9721 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
     9781 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
     9841 acaattttct tagcgcctgt cccattaggc tattttattt gtcagtttgg ccctgggttg
     9901 tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctgtc catgtccaga
     9961 ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat
    10021 tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaac
    10081 ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg
    10141 cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc
    10201 gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca
    10261 gagatgttta atcttggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc
    10321 cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg
    10381 aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg
    10441 ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt acatctgcgt
    10501 ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgctattgtg
    10561 atgcgaaccg gctggcagcc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg
    10621 ttgctgattg aagcggcgat gctagcgacc gatcgcgcac caggcttgca ccgtgggcgt
    10681 tggggcttta gcggctgggt gcagcatgat gaagctatct ggcaggaagt gaaagcggaa
    10741 gcgcaaactc gtgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac
    10801 agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg gattggtgaa
    10861 ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg
    10921 tacggcacag tgttgagcaa cccgccatac ggggaacgtc tggacagcga accggcgctg
    10981 attgcgctgc atagcctgct gggtcggatc atgaaaaacc agtttggtgg ctggaatctc
    11041 tctttgttta gtgcctcgcc ggatctgcta agctgcttgc agctgcgtgc agacaaacag
    11101 tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt tgccgaaagc
    11161 actccagaca gcaaaccggc gatggtagcg gaagactacg ccaaccgcct gcgtaagaac
    11221 ctcaaaaaat tcgagaagtg ggctcgccag gaaggaattg aatgttaccg cctgtatgac
    11281 gccgatctac cggaatataa cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag
    11341 gagtatgcgc cgccaaaaac tattgatgct cacaaagcgc gtcagcgtct gttcgatatt
    11401 atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct gaaaacccgt
    11461 gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga gtttcttgaa
    11521 gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga tactggtctg
    11581 ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg caaagatttc
    11641 ctcaacctgt tctcttatac cggtagcgcc accgtgcatg cgggattagg cggcgcgcgc
    11701 agcaccacca ccgtggatat gtcgcgtact tatctggagt gggcagaacg caacctgcgt
    11761 ctgaatggcc tgaccgggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg
    11821 cgtgaggcaa atgaacagtt tgatctgatc tttatcgatc cgccaacctt ctctaactca
    11881 aaacgaatgg aagatgcgtt tgatgttcag cgcgatcata tggcgctgat gaaagatttg
    11941 aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg tggcttccgt
    12001 atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg
    12061 ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc
    12121 tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg
    12181 cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg
    12241 gtcgcaacgg cgcgggcaaa tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg
    12301 atgacggtcg cattatttac gagcaagatt tgattgtagc tcgtctgcaa caggatccgc
    12361 cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa gaacaagcgg
    12421 aatatctgaa acgctatcac gatatttcgc gcctggtgat gaacgacccg agcgagaaaa
    12481 atctcaacga actggcgaag gttcaggaac agttggatca ccacaacctg tggcagctgg
    12541 aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaatgtt gcgctgtcgt
    12601 cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg agtaatccgc
    12661 gcgtgctgtt gcttgatgaa ccgacaaacc acctggatat tgaaaccatc gactggctgg
    12721 aagggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac cgttcgttta
    12781 tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg acctatccag
    12841 ggaattacga ccagtacctg cttgaaaaag aagaagccct gcgcgtggaa gaactacaaa
    12901 atgccgagtt cgatcgcaaa ctggcgcagg aagaggtgtg gatccgccag gggatcaaag
    12961 cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcaatgcgt cgcgagcgtg
    13021 gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg
    13081 gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt tgacggtaag caactggtga
    13141 aagatttttc ggcccaggtt ctacgtggcg acaaaattgc cctgattggt ccgaatgggt
    13201 gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcggac agcgggcgta
    13261 ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg gaactggatc
    13321 ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg gttaacggca
    13381 agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa cgggcgatga
    13441 cgccggtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg cgtttgttcc
    13501 tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat gtcgaaacgc
    13561 tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg gttagccacg
    13621 atcgtcagtt tgtcgataac accgttacag aatgttggat cttcgaaggc ggcggtaaaa
    13681 ttggtcgtta tgtcggcggt tatcatgatg cccgtggtca gcaagagcag tatgtggcgc
    13741 tcaaacagcc tgcggtgaaa aaaaccgaag aagccgccgc ggcaaaagca gaaactgtaa
    13801 aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag ctaccgcaat
    13861 tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaggtggcg gatgcttcct
    13921 tcttcagtca gccacatgag cagacacaaa aagtgctcgc cgatatggct gctgcagagc
    13981 aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa aatggtggct
    14041 gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg cctgatgaac
    14101 tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata gcgcatcatt
    14161 aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg
    14221 acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt ccccggtgtg
    14281 gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tccgaccgcc tatgcgttgg
    14341 ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg aacgttgcag
    14401 gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct gaggactacg
    14461 ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa
    14521 ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag caactggcac
    14581 gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgct ggtgtgctgg
    14641 tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc tttctccctt
    14701 ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt cgctggttat
    14761 gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc gtcacgggga
    14821 tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct gatgaacccg
    14881 tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg cagtggacac
    14941 tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg cccatcatgg
    15001 tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc attctgttat
    15061 ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt atggtgccaa
    15121 cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat ggcaagcgcg
    15181 acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc tggtcgatga
    15241 ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta
    15301 tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca atgttttctg
    15361 ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt
    15421 cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa gaactggtct
    15481 cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct tttttatcat
    15541 tacagccatc agggactgga agtgaccctg atcaccgcga atgcggaagg aattgaaggt
    15601 ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag cgccacactg
    15661 gctgatgatt tgacgcacgt tgaaatcaaa gtgcggctga attctggtat ggaaaaattg
    15721 ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga agggattagc
    15781 ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc gaaaggcagc
    15841 aaaatggata aatacgattt gctggactcg ccaccgttgg ccccgcctga tgcgaaaggt
    15901 atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga tccggtgctg
    15961 ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca aaaacgcaat
    16021 atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag caatgttcgc
    16081 ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg tgtggagatg
    16141 ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga aggtctggat
    16201 ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca gaagagcatt
    16261 caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga ttcggtacgc
    16321 ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac cgtaagcaaa
    16381 gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg tattccggta
    16441 ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt
    16501 gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg
    16561 gtcactggtg cactgtatgt tgatcttgat ttctatccaa atacgcctgc aataaccggt
    16621 attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct ggcgcaaatc
    16681 cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa tccgatgatt
    16741 gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg
    16801 ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc gacggatatg
    16861 cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg ctccgcagcc
    16921 tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg
    16981 gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa
    17041 gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt gcagcactgt
    17101 ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc
    17161 agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta gagcaggtca
    17221 ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat gtgaagtatg
    17281 tgattgccaa caacaacttg tgggccagcc cgttggatca acaattgcgc aacaccctgg
    17341 ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct ctgggaagcg
    17401 cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat ggcaaggtca
    17461 ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt ccgttccgtc
    17521 tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtactg gccagtgtct
    17581 ggagtcagga agccgcttct attgcgcaag agataaagcg tctaccttaa ttataaagat
    17641 ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt ctaacgtgcg
    17701 gaaaaagttg ttcctcttta cattttttgt acaaccgaca tacccgtgta gctcacaaat
    17761 atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta ttgcttaact
    17821 gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta tgagggaaac
    17881 gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt ggttatcagg
    17941 ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat caaaggtcac
    18001 aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc tgattctgtc
    18061 tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct gtctgattat
    18121 aatcagaagg cagaagtatc ctggaacaga ccgactttca ggtcgctggc ggtatagatc
    18181 aaacgaccat caaccagcac ttctccatcc gccaggccca taatcagacg acggttaaca
    18241 atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac ctgaccagtg
    18301 aatttcactt cgccaacgcc cagtgcgcgg cctttacctt cgccgcccag ccagccgagg
    18361 tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat aaccggatcg
    18421 ccaataaagt ggcatccgaa gaaccacaga tccggattga tatccagttc tgcttcaaca
    18481 taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc catcatcagc
    18541 atattcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgcg accagaggca
    18601 agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt aagccttatt
    18661 ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat cagttcggaa
    18721 taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc tgttgcgatg
    18781 cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg ccatcccaca
    18841 ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag atggtgaatt
    18901 tgccttcttc taccgctttc actagttcac tgtgaagact taaatggcga acgttagctg
    18961 tggggataat gacaccttgt ttcccggtta actcacgttg ctggcaaata gcaaagaagc
    19021 cttcgatttt ctcatttaaa ccaccgaccg gctgggcgcg accgaactga tcgactgaac
    19081 ctgtgatagc gatactctga ttcaccggca catcggcgag ggcgcttatc agggcgcaga
    19141 gttcagccat cgaggcacta tctccatcaa cttcactgta tgactgctca aatgtcagcg
    19201 atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac gcttgcatga
    19261 tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg atgtcggtga
    19321 attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg
    19381 gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg
    19441 tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg aggaaacctt
    19501 cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag gtgtcgccat
    19561 cacacaggga ggcgacctct ttacactggc ggaggatcca ctgcgggcta agcggaagtg
    19621 tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccag gcatccgctc
    19681 ccggtgcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac cactgggtta
    19741 ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata gcctgctctg
    19801 aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct accagaatga
    19861 ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac tcatcaaacg
    19921 caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac agcagaggtt
    19981 gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga tgcaccagac
    20041 caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt tgctccgctt
    20101 ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct gcactcactg
    20161 cgtgacgtaa gcggatggag tggccggaaa cctcatagtg accgcccacc agttggcctg
    20221 catcgctttg tagcgtacgc gcggcattgg caataagatt cagatactca gactcttccg
    20281 gggccttcgc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa
    20341 attgcagccg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct
    20401 gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat gcaagtttcg
    20461 taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg
    20521 cactacagca cacgtagaaa gcaccgacaa tactccaggc atgggcgtta aagctcacag
    20581 gatggagatt cttttcttca ctggcctaaa aagctgatat tctgtaaaga gttacacggt
    20641 aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg ttggaaatgg
    20701 aagtatctgg tgaagaagca tcgcgaaggg gagttaatca cccgttacat agaagccagt
    20761 gccgcccagg aagccgtcga tgtgttgctc tcgctggaaa atgaacccgt gctggtaaat
    20821 ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca aaccattcgg
    20881 gcaagacgaa agcgccattt taatgcggaa caccagcata cgcgcaaaaa atcgatcgat
    20941 ctggaattta ttgtctggca acgtctggct ggtcttgcgc agcgtcgcgg taaaacgctt
    21001 tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata cgcgaataaa
    21061 atgtcttctc tgaagcagga tctgcaggca ttgctgggta aggaataact gacgaaagtc
    21121 agttcaattt actaaaggca aaaaaaaccc cgcagcagcg gggtttttct accagacgag
    21181 aacttaagcc tgcggctgag ttacaacgtc tttgatacct ttaacttcga tctctacgcg
    21241 acgatccgga gccaggcagt cgatcagtgc agcacgctgt ttcacgttgt cacaggtgtt
    21301 gccagtaacc gggttggatt cgcccatacc acgtgcggag atcttgtctg ccgggatacc
    21361 tttggagatc aggtaatcaa caacagactg agcacggcgc tcggacagac cctggttgta
    21421 agcgtcagaa ccgatgcggt cggtgtaacc cagaacaact acggaaccgt ctttcggatc
    21481 caggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg gtttcagggt
    21541 tgctttgttg aagttgaaca gaacgtcaga cttcagagtg aagtgcttgg tctgtacttc
    21601 cggtgccgga gctggagccg gagcaactac tggagctgct tcgccctgac cgaaacggta
    21661 ggaaaccccc aggctcagca tgccgttgtc cggacgagtg ccgatggtgt gtgcgtcacc
    21721 gatgttgttg gtccactggt attccagacg ggtagcgatt tcaggagtga tcgcgtactc
    21781 aacaccgcca gcgaagaccg gagaaacgcc ggtgtcgtgg tttttaccat aaacgttgga
    21841 tttagtgtct gcacgccata ccataccacc cagacgagtg tagatgtcca ggtcgtcagt
    21901 gattgggtaa cccagtttag cggtcagttg aacgccctga gctttgtatg caccgttttc
    21961 aacgctgcct ttgtacggca tacgacctaa ccagtcgtaa cccatttcaa agccaacata
    22021 cgggttaacc tggtaaccac caaaagcacc agcgcccagt tggttttcat gggtcgggcc
    22081 attgttgttg atgaaaccag tgtcatggta ctgggaccag cccagtttag caccagtgta
    22141 ccaggtgtta tctttcggag cggcctgcgc tacggtagcg aaaccagcca gtgccactgc
    22201 aatcgcgata gctgtctttt tcattttttg cgcctcgtta tcatccaaaa tacgccatga
    22261 atatctccaa cgagataaca cggttaaatc cttcaccggg ggatctgctc aattaactct
    22321 accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca acgtagttga
    22381 aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat aaggtatgtt
    22441 taatcttttt tgtcagcgac aatttacaga agaaaatcgc ggaaaccgct tcagacaagc
    22501 ctccgcaagg aaaattagtc acgactgaaa gcattggctg ggcgacaaaa aaagttccag
    22561 gattaatcct aaatttactt aatgatacaa attagagtga atttttagcc cggaaagttg
    22621 tctcgtggcg tgagaggatg cgcttaccgg acgcataata aaccccatag cgttaccttc
    22681 atttgccgca tcaacaagtt cagcatgctc ttcttcagtc aaatcatctg ccaaccaacc
    22741 gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc attgactcca cagtgtggca
    22801 aggggagagc tggctaatct gcattacttt cgttaagggt agcccagatg cctgaaccca
    22861 ttcccgactc agtttttgtt gcggtgttaa ccagagttgc cagcgcgatt gctgaccgag
    22921 ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg ggctgatctt cgcgatagac
    22981 aacttcactg ataagcccgg ctgtagtgtt ttccgtagag acacgcgcaa ttttacttgc
    23041 tgcggatgag aacgacgaag aacgatgtgc atagcctgaa gtgtacataa tcaatccagc
    23101 ccctgtgagt tactgtatgg atgtacagta catccagtga caacaaagat caaccctatt
    23161 ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga aaacataaat taatcttgcc
    23221 ccttaagaat aagttgccta ttttcgtagt taacggatcc gttaatgtga atcattcttt
    23281 tatgttatga ttttaaaagg aattttatga aaagcctctc ctataagcgg atctataaat
    23341 cacaagaata cctggcaacg ttgggcacaa ttgaataccg atcattgttt ggcagttaca
    23401 gcctgaccgt tgacgacacg gtgtttgcga tggtttctga tggtgagttg tatcttcggg
    23461 cttgtgagca aagtgcacag tactgtgtaa aacatccgcc tgtctggctg acatataaaa
    23521 agtgtggccg atccgttacc ctcaattact atcgggttga tgaaagtcta tggcgaaatc
    23581 aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc agcgctgaaa gagaaaagca
    23641 cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat gtcttttcat ctggaagcga
    23701 ttctcgggga ggtggggatt aaggatgtac gggcgttacg tatacttggg gcaaaaatgt
    23761 gttggttgcg actgcggcag caaaacagtc tggtgacaga aaagattctg tttatgcttg
    23821 aaggtgccat tatcggcatt catgaagctg cgctcccggt ggcacgccgc caggagcttg
    23881 cagaatgggc tgactctctt acgccgaaac aggagtttcc tgcggaactt gagtaatctg
    23941 gcgttgcaga cgaccaatct caggcagtaa tgcaatcaat aatccaactt gttgtacgac
    24001 cagaggttct ttgctgtctg cccgtggttc aagttgctgc atccgctgtt tcaggctagc
    24061 taatgcctca ttgacgcgtt cttcatcagc aggttgatga tgtaacgcgt catcaacata
    24121 gcaaactgcg tcatcaagaa acgccagaat ttcaggatta gttaactgct cccggtgagc
    24181 accgagggct gagatatagc tggtaaacgt atggttaagg cacagcaacc gaaacgcggc
    24241 ttcgcgaatt tgcggggtaa cgttcggctc gctggacata tttgatacca ccgacgccag
    24301 ctcagcatca cggttgtgtg catcgcggcg ggcaatacga tacgccagac ggttatcacg
    24361 cccctgatgg tattgctcca gtatggcatc gagataccga cagttggcct ctgtggcgcg
    24421 ttcgagcatg cgcggcagat tgcgaaactg ccagtcaggc cagatgtagc tcactgccgc
    24481 ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga ggtaacgcta cttcaaaacc
    24541 ttcacccagt aagttaaaac acagtagcac caaaagtgtg atgaacatcg ttgcatgagc
    24601 gtattgcacg ttacggaagg caaaaaagag cacgccggta ataaccagca gcaccagctg
    24661 cccttccagt gatggcacaa accacagcac aggaatgcca atggcgatac ctaccagcgt
    24721 accaataatc cttaacttca ggcggtggcg cgtggcgtta tagtttggct ggcagacaaa
    24781 caaacttgtc agcaagatcc aatacccgtg atgcattccg gttatctgaa tgatggcgta
    24841 gccgaagcac aacaccagcg acattcttac cgcatgacgg aagagggcgg attccggcgt
    24901 gaagtgacgg ctaagacgca gccagatatc actcaacccg tgcgggctgt catcagcgag
    24961 ctcattttcg tcattattat ggggtagtgc ctgggcctgt tctgattcaa ttgttgccag
    25021 ttgggcatca atggcgcgta aattgttcag caaaaatccc agtgttttga gtaaatcggc
    25081 gggtgcgccg ttatcgcgca tccgctccag cgcagcatca atatgcgtaa aagcgcgctc
    25141 aaaatgcgga tcatgttgat aaggctgacg caacaaaata cagcgtgaca gttgctggca
    25201 cgcctggccc tgcatcgaca tcagccgctg aaaacggaac agcacgtcgc tgtggcgaaa
    25261 atgttcacgc aatgtttgat actgaatatg agaagagctg gcacgctcgt gaatatcctg
    25321 tgcgacaaag taataatgca gcgtgcgacg cgttccccgt tgaccacgat cgccacgtaa
    25381 gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc atcagcagac cgttggcgag
    25441 agccaaatcg tacagcggtg cctggctttc atcttcaata tcaggatcaa acatgcgcga
    25501 cttgagctca agataacgcg ccagttgttc atagcaacgc gccaggttgt cctgcagcgg
    25561 gcggaccggg aacagcagat gaccaataag tgtcaggacg ttgtaccaga cggcaccggc
    25621 cagcagatac atcggctgct gataccagtg ctcatacagt gatgttccca acatagtgta
    25681 aatggcgatc agcaatgcac cgaaggcaat tgttgcatag cgttgaccca gaccgccgag
    25741 caaaatgaag ccgctggtag agagcgttaa gccaatcgca aatagccagg gccagggaaa
    25801 cagcaattct actgaggccg aggcgataaa aaagcagaac agcgtaatga tgaggttacg
    25861 caaacgtccc gccagtcggt catcgagatc ggtcagcgcc gctgccacca tccccagcgt
    25921 tagcggaatc gtcagtttta catcacccag ccaccacgga aacgctgtgg ttccacaaag
    25981 cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc caggtatagc gtttgagcaa
    26041 aggacttagc ataaaggcca tattagtgga ttattcaaaa cgacgacgtg cattggcttc
    26101 acgcgcagct tgcgctgttt ctaccgatac cacgcgacga ccaaccggcc atagtgcaat
    26161 agcggcaatt ttaaagttcg caatgccgac aggaatgcca atgattgaaa tacattgtgc
    26221 gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca aagaaaatca gccagaaaat
    26281 attcaatacc gtaccgccag tattcagcag cacatttttg ccagccgggt tcagttcatc
    26341 gacatgaata gcttcattgc cataaggcac cagagatagt ttagtgatct cccagcagga
    26401 tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc agagtcgcca acagccagcc
    26461 cagagtggtg gcaaatccgc caagcacaaa gttcagaatg ttcaaaacgg tacgcataaa
    26521 acctcgcttt actgtggttt tcagtaatgg gcggcaattg taacgttttt ttgggctgga
    26581 gcacgttttc tctgacggtt acactgataa gaaataattt cgtgtggatc tacagagtca
    26641 tggaactgaa agcgacaacg cttggaaaac gtctggcaca gcacccttac gatcgggcgg
    26701 tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca cgaatacctt attcctttca
    26761 atcaattact ggcgattcac tgtaagcgcg gtctggtatg gggcgagctg gaatttgtac
    26821 tgccggacga aaaagtggtg cgtctgcacg gcaccgaatg gggcgagacg cagcgttttt
    26881 accatcatct tgatgctcac tggcggcggt ggagtggcga gatgagcgaa attgcgtctg
    26941 gtgttttacg ccagcaactg gatttgattg ccacgcgcac tggagaaaat aaatggctga
    27001 cgcgtgagca aacctctggc gttcagcaac aaatccgcca ggctttgtcg gcgttgccgt
    27061 tgccggttaa ccgactggaa gaattcgata actgccgtga ggcgtggcgt aaatgtcagg
    27121 cctggttgaa agatattgaa agcgctcggt tgcagcataa ccaggcgtat accgaagcca
    27181 tgcttaccga gtatgcggat tttttccgcc aggtcgagtc ttcaccgctg aatccggcgc
    27241 aggcccgggc agtcgttaat ggcgagcatt ctctgttagt gctggcaggt gcaggaagcg
    27301 gaaaaacgtc ggtgctggtg gcccgtgcag gctggttgct ggcgcgtggt gaagcgtccc
    27361 ctgagcaaat tttattgctg gcgtttggtc gcaaagccgc tgaagagatg gacgagcgga
    27421 ttcgcgaacg gctacatacc gaagacatta ccgcacgcac gtttcatgcg ctggcgctgc
    27481 atattattca gcagggcagc aaaaaagttc cgatagtcag caaactggaa aatgataccg
    27541 ctgcccgtca tgaactcttt attgctgagt ggcgcaagca atgcagcgaa aagaaagcgc
    27601 aggcgaaggg ctggcggcaa tggctgacgg aagaaatgca gtggtcagtg ccagaaggta
    27661 acttctggga tgatgaaaaa ttacagcgtc gccttgcctc tcgcctcgat cgctgggtaa
    27721 gtctgatgcg gatgcacggt ggtgcacagg cagaaatgat tgccagtgca cccgaagaga
    27781 ttcgcgatct gttcagtaaa cgtatcaagt tgatggcccc gttattaaaa gcctggaaag
    27841 gtgcgctgaa ggcagaaaac gctgtcgatt tttcgggcct tattcatcag gcgattgtga
    27901 ttctggagaa aggtcgcttt atcagcccgt ggaagcatat tctggttgat gaatttcagg
    27961 atatctcgcc gcagcgggca gcgttgttag cggcattacg caagcaaaac agtcagacga
    28021 cgttgttcgc tgttggtgat gactggcagg cgatttaccg attcagcggt gcgcaaatgt
    28081 cgctcaccac cgctttccat gaaaactttg gtgaaggcga acgttgtgat ttagacacga
    28141 cttaccgttt taacagtcgt atcggtaagg tggcaaaccg gtttattcag cagaacccag
    28201 gccagctgaa aaagccgcta aacagcttaa ccaatggaga caaaaaagcc gtcacgttat
    28261 tggatgagag tcaacttgac gctttgctgg ataagctctc tggttatgcc aaaccggaag
    28321 agcgcattct gatcctggcg cgttaccatc acatgaggcc tgccagcctg gaaaaagcgg
    28381 caacacgctg gccgaagttg caaatcgact ttatgaccat tcatgccagc aaagggcaac
    28441 aggcggatta cgtcatcatc gttggcttgc aggagggaag tgatggtttt ccggctgcgg
    28501 cgcgggagtc gattatggaa gaggcgctac tgccaccggt tgaggatttc ccggacgctg
    28561 aagaacggcg gttaatgtac gtggcgctga cccgggcacg ccatcgggta tgggcactgt
    28621 ttaacaaaga gaatccctct ccctttgtgg aaatactgaa aaatctggat gtgccggtgg
    28681 cgagaaaacc gtaagaaaca ggtggcgttt gccacctgtg caatattact tcagacggtc
    28741 cgcgagataa cgctgataat cggggattag aatatcgacc gcgtcgttga aatgcggcga
    28801 ctggattatg aagtctgccg ttgccacgtt ggtggcgacc ggaatgttcc ataccgtcgc
    28861 cagacgcagc aaggctttca cgtcaggatc gtgcggcacg gcatttagtg gatcccagaa
    28921 gaaaatcaat acatcaattt tcccttctga gatcaatgcg ccaacctgct ggtcaccccc
    28981 cattgggcca ctcaacatcg cgttgacgtt catgccggtc gcgcgggaaa ttaagttacc
    29041 ggtagtgcct gttgcataca gtacgtgttg ttccagtaac ggttgatgcc gttccaccca
    29101 gctcatcagc atttgtttgc agtgatcgtg tgccaccagc gcaatatgtt tccgcgcagg
    29161 taaagtgcga gtcgtcagtt ccataatgta catccgtagt taactttcct acagattact
    29221 gtaagcactt atcgctgcaa gataaagacc gaaaaagcct gcgcacaggc acaaaaatct
    29281 caggaagatg gttgtttttc cgcccactgc aggaaagtat ttcgcgtttg tgggtcagcc
    29341 agtttaaacc aatacttcag ccgttgttct gtgagcacct gagactgcgg tggaatagca
    29401 tccagctcgg aaacacccga aagcagcgta ctgtcatctg ccatcatcgt ggcaaattgc
    29461 ggtagtgaag cgcgtttcgc ggatttgtta tagcgttcaa cctcgacctc ataatcaatc
    29521 gtttttgccg ttgaggtaat ggcgagaata tccagcttta ccggaatcgg cgtcgcatcg
    29581 ccatccaaca attcaaggcg cggcgcggca tcaaaatggt tagcttcccg ctcattctcc
    29641 aggcgaggca ggcgaaaatt gacctggttg atgagctggg tattaaaact gaccaccagc
    29701 ggtggggaga tatacagccg ttcttcacta ttggaaagat gaatcgtctt ctcaacgcga
    29761 aacactaact gatgcggtcc attatccagt tcaatgctgt ccgcaccgcg cagcagagag
    29821 ctggaaacct tcttgccgtc gagtaccaga agatcgacat cggttgaaag ccgcagcgtg
    29881 gtggcaaaaa cggataccgg caaacataat gcaattaagg tggtcacgat gccggttttc
    29941 ataggtagct cctgtcaaaa gaccgcccga ataataatgt gtcaaaattt ttctttaatc
    30001 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
    30061 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
    30121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
    30181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
    30241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
    30301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
    30361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
    30421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
    30481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
    30541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
    30601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
    30661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
    30721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
    30781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
    30841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
    30901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
    30961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
    31021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
    31081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
    31141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
    31201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
    31261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
    31321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
    31381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
    31441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
    31501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
    31561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
    31621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
    31681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
    31741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
    31801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
    31861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
    31921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
    31981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
    32041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
    32101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
    32161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
    32221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
    32281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
    32341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
    32401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
    32461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
    32521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
    32581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
    32641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
    32701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
    32761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
    32821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
    32881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
    32941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
    33001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
    33061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
    33121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
    33181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
    33241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
    33301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
    33361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
    33421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
    33481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
    33541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
    33601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
    33661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
    33721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
    33781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
    33841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
    33901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
    33961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
    34021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
    34081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa
//
LOCUS       DAQSWQ010000040        33891 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.040, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000040 DAQSWQ010000000
VERSION     DAQSWQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.040"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(1..1599)
                     /locus_tag="R8G18_001965"
                     /pseudo
     CDS             complement(1..1599)
                     /locus_tag="R8G18_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            complement(1809..2327)
                     /locus_tag="R8G18_001966"
     CDS             complement(1809..2327)
                     /locus_tag="R8G18_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HEE9795671.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            3024..3524
                     /gene="tssB"
                     /locus_tag="R8G18_001967"
     CDS             3024..3524
                     /gene="tssB"
                     /locus_tag="R8G18_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEE9795672.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            3559..3783
                     /locus_tag="R8G18_001968"
     CDS             3559..3783
                     /locus_tag="R8G18_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795673.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            3834..5309
                     /gene="tssC"
                     /locus_tag="R8G18_001969"
                     /pseudo
     CDS             3834..5309
                     /gene="tssC"
                     /locus_tag="R8G18_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            5316..5729
                     /gene="tssE"
                     /locus_tag="R8G18_001970"
     CDS             5316..5729
                     /gene="tssE"
                     /locus_tag="R8G18_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEE9795674.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            5733..7583
                     /gene="tssF"
                     /locus_tag="R8G18_001971"
     CDS             5733..7583
                     /gene="tssF"
                     /locus_tag="R8G18_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEE9795675.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            7547..8629
                     /gene="tssG"
                     /locus_tag="R8G18_001972"
     CDS             7547..8629
                     /gene="tssG"
                     /locus_tag="R8G18_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEE9795676.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            8654..9934
                     /gene="tagH"
                     /locus_tag="R8G18_001973"
     CDS             8654..9934
                     /gene="tagH"
                     /locus_tag="R8G18_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HEE9795677.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            9931..10455
                     /gene="tssJ"
                     /locus_tag="R8G18_001974"
     CDS             9931..10455
                     /gene="tssJ"
                     /locus_tag="R8G18_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEE9795678.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            10458..11789
                     /gene="tssK"
                     /locus_tag="R8G18_001975"
     CDS             10458..11789
                     /gene="tssK"
                     /locus_tag="R8G18_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEE9795679.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            11794..12555
                     /gene="icmH"
                     /locus_tag="R8G18_001976"
     CDS             11794..12555
                     /gene="icmH"
                     /locus_tag="R8G18_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEE9795680.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            12564..15347
                     /gene="tssH"
                     /locus_tag="R8G18_001977"
     CDS             12564..15347
                     /gene="tssH"
                     /locus_tag="R8G18_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEE9795681.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAEPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            15344..16087
                     /gene="tagO"
                     /locus_tag="R8G18_001978"
     CDS             15344..16087
                     /gene="tagO"
                     /locus_tag="R8G18_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HEE9795682.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            16092..17504
                     /gene="tssA"
                     /locus_tag="R8G18_001979"
     CDS             16092..17504
                     /gene="tssA"
                     /locus_tag="R8G18_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEE9795683.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            17583..20324
                     /gene="tssM"
                     /locus_tag="R8G18_001980"
     CDS             17583..20324
                     /gene="tssM"
                     /locus_tag="R8G18_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEE9795684.1"
                     /translation="MPRFKVSAFWLLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANR
                     WLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLR
                     LQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLRKGFPSDIIYAPEGARGAEQRLYLTPH
                     VGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLT
                     ADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAI
                     LGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGS
                     REPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWP
                     LVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHH
                     YYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRL
                     ADMGLYQGRRIGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRM
                     LEDKSGRNNQVVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDA
                     ISRWTPYDKPVVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFT
                     SADDNKLVVPQFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQH
                     QLTEQYISDYTATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDSTQ
                     PGVFSEKLSAKEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTE
                     LHRYLLAIQNAPVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTD"
     gene            20361..21047
                     /locus_tag="R8G18_001981"
     CDS             20361..21047
                     /locus_tag="R8G18_001981"
                     /inference="COORDINATES: protein motif:HMM:NF018454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795685.1"
                     /translation="MEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDG
                     ILDTFYQQNLKLFIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSF
                     AVETVSLSGNKRRSVLNLDSQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNA
                     PRSISFSGPWAQFRLFWAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLF
                     SQFGLSDTLY"
     gene            21058..22410
                     /locus_tag="R8G18_001982"
     CDS             21058..22410
                     /locus_tag="R8G18_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HEE9795686.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            22434..22916
                     /locus_tag="R8G18_001983"
     CDS             22434..22916
                     /locus_tag="R8G18_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HEE9795687.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            22960..23887
                     /locus_tag="R8G18_001984"
                     /pseudo
     CDS             22960..23887
                     /locus_tag="R8G18_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23897..24376
                     /locus_tag="R8G18_001985"
     CDS             23897..24376
                     /locus_tag="R8G18_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795688.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(24513..25298)
                     /locus_tag="R8G18_001986"
     CDS             complement(24513..25298)
                     /locus_tag="R8G18_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795689.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(25628..25704)
                     /locus_tag="R8G18_001987"
     tRNA            complement(25628..25704)
                     /locus_tag="R8G18_001987"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25668..25670),aa:Asp,seq:gtc)
     gene            complement(25837..26568)
                     /gene="dnaQ"
                     /locus_tag="R8G18_001988"
     CDS             complement(25837..26568)
                     /gene="dnaQ"
                     /locus_tag="R8G18_001988"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEE9795690.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            26633..27100
                     /gene="rnhA"
                     /locus_tag="R8G18_001989"
     CDS             26633..27100
                     /gene="rnhA"
                     /locus_tag="R8G18_001989"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEE9795691.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(27097..27819)
                     /locus_tag="R8G18_001990"
     CDS             complement(27097..27819)
                     /locus_tag="R8G18_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEE9795692.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            27853..28608
                     /gene="gloB"
                     /locus_tag="R8G18_001991"
     CDS             27853..28608
                     /gene="gloB"
                     /locus_tag="R8G18_001991"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEE9795693.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            28680..30038
                     /gene="mltD"
                     /locus_tag="R8G18_001992"
     CDS             28680..30038
                     /gene="mltD"
                     /locus_tag="R8G18_001992"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HEE9795694.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(30087..30683)
                     /locus_tag="R8G18_001993"
                     /pseudo
     CDS             complement(30087..30683)
                     /locus_tag="R8G18_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(30714..31514)
                     /locus_tag="R8G18_001994"
     CDS             complement(30714..31514)
                     /locus_tag="R8G18_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEE9795695.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            31755..32669
                     /locus_tag="R8G18_001995"
     CDS             31755..32669
                     /locus_tag="R8G18_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9795696.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(32666..33469)
                     /gene="dkgB"
                     /locus_tag="R8G18_001996"
     CDS             complement(32666..33469)
                     /gene="dkgB"
                     /locus_tag="R8G18_001996"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HEE9795697.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(33632..33708)
                     /locus_tag="R8G18_001997"
     tRNA            complement(33632..33708)
                     /locus_tag="R8G18_001997"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33672..33674),aa:Asp,seq:gtc)
BASE COUNT         8428 a         8771 c         8732 g         7960 t
ORIGIN      
        1 ctggttattg gtaacggtga tcgcctggtt gttacctatt ttttcggcat ggttgtttat
       61 cacatccgtg gtgcggttat tcagcacctc ggtgttcatg ttcttctgca cgtggatgta
      121 gacctgttct ttccctgtcg catcgtcaaa cttcagttca ttaaacccgc tgcccttata
      181 ggttttcgaa cgaatggtca tctgcgtctt cgtccccggc aggctaccgg gtgtgcggtt
      241 ttcctggtgg taggtacgcc ccataataat cggctggtcc ggatcgccgt tgaggaagtc
      301 cacaatgacc tcctgaccca cgcgcggtat cgcaaggtta ccgaatccgg tgcctgccca
      361 cgcctgtgcc acacggatcc agcatgaact gtcctggttt gacgggttat agcggtccca
      421 gttaaatttc acccgcacgc ggccatgttc atcacagaag atttcctcgc ctgccggtcc
      481 cgtcacgacg gcgctctgcg ggccatccac cagcggtttc agcaacggct gtggccgcca
      541 tgtccggtct gccggtatta ccgcaaagtg gttattcagc gtggttcctg aaccactgcg
      601 tcccggcacc gcctgcggct gctcgccgtg cagttcactt gccaccacct gccattcccg
      661 gttcaggttc gcctgcggat gccccgtcag cacaattcgc cgtcccggcc atatctccgg
      721 agaacggctt gttccgcgcg ccacttctgc gttgttgcgc cagccatcca tctgccagcg
      781 ggcaaagttc tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg
      841 tgtgcgctgg taatcctggt actggccttc ctgatcaaaa cgccctgccc agccgggtcg
      901 tttaaaggtg tagtctttgg tcaccacgga agaaggacgg atttgtgcgc tgtagcggaa
      961 ctggctgatg cagaaggtac ttacctcggt acgggtgttc gggttccagg ggatctcaaa
     1021 ggactccggc agataacgca cggtgtcgca caggaccagg ctctggtcgg tacttttttg
     1081 cgcgtgctcc tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta
     1141 atcagtttcg ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg
     1201 actccactcg gtcacaccgt tttccttcag gatcgtcgcg aggatgcttt cgatgtcctc
     1261 attctggaag atacggaagt tctggcgcag ccctgtgcgc cacagcggtg ggcatacctt
     1321 catgctgtac agcatctggt ttttgtcgtt ctcccccagt tcaaactagg tcaccacacc
     1381 tttcacccgg cgctgtacgt catcgccctg ccatatcgtc aggtaggcca ttttgtccag
     1441 tacctgcgga aattcaaggg agagaaactg ctggctgacc agagagagct cgagggaaaa
     1501 gagcgaagag agtgactggt tcagatgaaa ggagaccacc gcaaaagcat ccggtggcag
     1561 gccgtccact tccagcgtga aacgtaatcc ggttgacata gcaacctccg gggttaagga
     1621 aaaacagaca gttcgctgtt aaagcggctg tgacagtaca gtgttacgct tgcgttcagg
     1681 cacgctaccg ccgaagtttc cggcatccgc catctcccgt gaacgtaacc ccttactgac
     1741 aacctttctt tctacggcgt aatacaaaaa cagcatccgg cctgatagcc ggatgctgtt
     1801 ggcttaactt atgcttccag tggtgcgcgc cagtcgtcgg caccggaggt gccagatttg
     1861 acgtgttccc attcaatttt gcggtaggcc agtgaaacct cgagcaactg ggtgaactcg
     1921 cgtttcgccg ggtcctggca atgcggcata tgcagtttca tatcgacaat cgtcgaatca
     1981 gtcaggcggg tagtgaaata atgctcctgt ttgccctcca cagaggtacg ccaccagtga
     2041 agttcagtgg ttggcagcat ttcgccggag gccagtgcgt tgtacatcag cggaaccgct
     2101 ttgttcagcg ccacggtaaa aatgaaaggc ttgtgagcac gctgacccgc aggctgaccg
     2161 gactgaggag tggtcgggac agtaacgtta tgcaggaatt cctgcaccag catctcgtct
     2221 tcgtgcccct gcacatagat gttgccgaca gaatcagcgg taaatgcacc tgcagtaata
     2281 tttccctggg tctgaccagt gatggagata taacacggtg ttggcatttg taaactcctt
     2341 gttaacgtgg ttaattacta cttacaatga cctgccgtta atcccagaca ggcagatgca
     2401 gctcacttca cggaaccgca tcatttttca gtatttttta catacaaacc gagctagaat
     2461 ttacggttgt cgtaaaaact atttttaaaa tcattaaatc tcttattcag caataactta
     2521 ataatatata tctggataac catacccaac tgttcaaagt ccgtattgcc tccataaaat
     2581 ggccaaagct gaatcatgaa taaatattgt atttatcctt tctgaattat cattttgata
     2641 atttttgaga tattccttaa aaatacccac ttaatcaatc acaaaacaaa tatataaaca
     2701 agcaatcatt agattagctt atttttaatg aaattattat atacttcaat aagttacaca
     2761 aaaactctat atttaaatta ttgccataat acacctgacg cgaattgcac aggttcacac
     2821 aatctattta acgcttgcag aatacccctc tttacgatcg ataaaaagtc cttttcttat
     2881 ttattttgaa gcgtaaaatg cggggtgatg aaggagtggt gattttattt tcaccgccat
     2941 tagggcggca ttgccgatat ttttttgcgg tacaaatatg ccgtgttcat ggcctgtacg
     3001 agataacatg acggatcact tttatgagca aaaaatttga aggcagcgtc gcgccccgcg
     3061 agcgtattaa tatatcgtat gtcccaaaaa cagatggcca gacggctgaa gtggaacttc
     3121 cgcttaacat gctcgttgtc ggcgataccg gaaatacgca ggaaacatcc ccactggatg
     3181 aacgtcaggc agtttccgtt aacaagcaca atttcggtgc agtgatggct gaagcagcca
     3241 ttggcctcaa ttttactgtc cccgccacgc tgaagggaag cacaacagac gatgaactga
     3301 atgtggcgct gaatatcaaa tcactggatg atttttcacc agatagcgtg gcccggcagg
     3361 ttccggaagt gaataaattg ctggaactgc gtgaagcact gactgcactg aaggggccga
     3421 tgggtaacct tccggcattc cgtacccagc tccaggcatt gctggaaaat gaagagtccc
     3481 gcgagcagtt gctcaaagaa atcggtctgg tgagcaataa ataaccacca ctatttcatt
     3541 aaacagggtt atccaggcat gacggcacac aaaaatatct ccgggacata ccacctctct
     3601 gttgcagaca ttctgcaggt ggtttaccag gtatgtttca gcccgtccgt tgaaatcaac
     3661 caggatggtg tggctgcgct gattacaaca ctggatcgcc gtatcagtga tctattggat
     3721 gagattatcc atttttgtga gtttcagcag agcgcgtcgc actggcagcg cgtacttcac
     3781 tgatggcgaa aagccggttt cagaaacaca gctaaaacag aaggattttc agtatgtctt
     3841 tacaggaaga ggaactcgtt tccagtcatg ccgggcagcc tgagcaggca tcgtcgctgc
     3901 ttgatcaaat catggcacag acccgtattc agcccggttc ggaaggttat gacgttgccc
     3961 tccagggtgt gacagccttt attgccagca tcctgcaatc cactgcatct gccgaaccgg
     4021 tcaataaact ggccgtggac agcatgattg cagatattga tgaacgcatc agccgccaga
     4081 tggatgtcat catccatgcg cctgcctttc agcaggtcga gtcgttctgg cgctcactga
     4141 aaaccatggt ggatcgcgtt gatttccgtg aaaacatcaa ggtcaacgtg cttcatgtca
     4201 ccaagcagga acttctggaa gactttgagt ttgcgccaga aattattcag tccggatttt
     4261 ataagcacgt ttattcgtcc ggtttcggac agtttggcgg taaacccatc gctgccgtgc
     4321 tgggcgctta tgagttcaaa aataccgcgc ctgacatgaa actgctgcaa tacgtcagtg
     4381 ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt ttccccggag tttatggggc
     4441 tgaactcgtg gaccgaactg cctaatatca aagatcttta tgccatcttt gaaggtccgg
     4501 cctacaccaa atggcgcgcc ctgcgtgact cggaagattc gcgctattta gggcttacgg
     4561 caccccgctt cctgcttcgc cagccctatt caccaacaga taaccctgtt aagaacttta
     4621 actattacga agatgtcagc cagaaccacg aagattatct gtggggtaat acggcctgga
     4681 tgctggcatg caatatcgca gacagttttg ccaaataccg ctgatgtcca aacattatcg
     4741 gtccgcaaag cggcggcgca gtgaaagatc tgccgatgca tctgttcgaa acgatggggc
     4801 agattcaggc caaaatccca accgaggtgc tggtcaccga tcgccgcgaa tttgaactgg
     4861 ctgaagaggg ttttatcacc cttaccatgc gtaaagactc tgataatgca gcctttttct
     4921 ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa agatgcagaa accaactata
     4981 agctgggtac gcagcttcct tatctcttca tcatcaatcg gttagcgcac tacatcaagg
     5041 tgttgcagcg tgaacaactg gggtcatgga aagaaaggag cgacttagaa agagagctta
     5101 atacctggat ccgtcagtat gttgccgacc aggaaaaccc gcctgcagac gtgcgcagcc
     5161 gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt agaaggcgaa cccggctggt
     5221 accaggttgc gctaagcgtg agacctcatt ttaaatttat gggggcaaat tttgagcttt
     5281 ccctggttgg ccggttagac agggagtaac acaccatgat accggatcgg gaaagaacca
     5341 caggttctct ttttgagcgt atggaagcct cttcggccag aaaccggcag ggaggcagta
     5401 ttcattcgct caggcaatcc attcgccaga acctacgaaa cattcttaat acccggtccg
     5461 gtagttgccg gggtgcgcct gagctgggca ttgatgaacc ggaaggggct gaaaacttcc
     5521 gcgaatccat gagtcgggcc atcgagcaat gtatagaacg ctatgaaccc cggatctcac
     5581 acgctgaagt gcaggcagtg gtttcatccg cctccagccc gctggatatg acgtttcata
     5641 ttacagcctg ggtgacattt aacgaaacgc atgaggtgct tgaatttgat atggcaccga
     5701 atggcagcca gcattaccgg gtggactgac aaatggaatt tgaagaacgc tatttccggg
     5761 aagaactcga ttacctgcgc cagcttagca agctgctggc aacggaaaaa ccccatctgg
     5821 cccgcttcct ggccgaaaaa gatgcagatc cggatattga acgcctgctg gaaggggtgg
     5881 cttttcttac cggcaatctc cgccagaaaa ttgaggatga attcccagaa ctgacgcacg
     5941 ggcttattaa gatgctatgg cctaattacc tgcgtccggt tccggcaatg acccttattg
     6001 aatatacgcc ggatatggat aagtcttctg taccggtgtt aatcccccgt aatgagcagt
     6061 ttacaaccaa cgccggggaa atcagagttg atgaagtgct gccctctgat gctaaaaagg
     6121 aggagccgcc tccctgtacc ttcaccctct gccgggatat ctggctgctg cccgttcgcc
     6181 tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt tatcaacatc accttttcgg
     6241 tcgcaccggg aacggacttc cgcacgctgg atctgaacaa acttcgcttc tggctcggca
     6301 atgacgacaa ctatacccgt gaccagcttt atttatggtt ctgcgaatac ttgcagggtg
     6361 ccgacctgac tgtgggtgaa cagcatattc gcctgcctga gtttatgcta aaagctgtcg
     6421 gttttgagcc gcaggatgcc atgctgccct ggccgaaaaa cgtccacagc ggctaccgga
     6481 tccttcagga gtatttctgt taccccgatg cgtttctctt ttttgatctt tgtggttgtc
     6541 cggctttgcc tgacggattg caggcggaat tctttaccct gcaactgcgt ttttcgcgcc
     6601 ctttgcccgt ggacatccgg ctgcgccgcg attccctgcg cctgtattgc gcacctgcca
     6661 ttaatttatt tatccaccat gcagaagcca tcacgctgga caaccggcgg gcagactatc
     6721 cgctggttcc cagccgccat tacccacaac attacgatgt attttccgtt aacagtgtgg
     6781 tgagccaggt ccaggatatg ttcaggaaaa aagatctggg gcgtcctgtt tcgacgcagg
     6841 ccgcgcgcca gtggccagcc tttgaaagtt tcagccatca gatagaatac agccggaagc
     6901 gggaagtggt gtactggcat caccggacca aaacatccct gttccatcgc ggctttgatc
     6961 atacccttgc ctttatacat gctgatggca gttatccgtc agacgaatct ctgctcagta
     7021 atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga gcttccgtca caaattcgtt
     7081 ccggcgatat caccggcaca accggaaaaa atgcggctgt tgcttcattt cgcaacatta
     7141 cccgcccgac gcaaccactc tggccggtca ttgatggcag cctgcactgg cccctactct
     7201 ccgccatgaa cctgaattat ctgtcattac tggatacgga cgcgctgaag caggtcatcg
     7261 ccaactttga tcgccacgca atccatcatc cgcagacggc acggctgtca caacaaaagc
     7321 tggatgccat tgagcgtctg gagacccgcc ccgttgatcg cctgtttacg ggtattcccg
     7381 tccgggggct ggcttccacg ctgtatctgc acccggagcc gtttgtctgt gaaggggaaa
     7441 tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct gtacgccagc gttaactcat
     7501 tccacatgct gaccgttgtg aacacagaaa gccaggagac atggaaatgg acggaaagaa
     7561 tcgggcagca tcctcttatc tgagtccggg aaatcccccg gcagataaag agcagaatga
     7621 tccgctggca caggttttcc ataatgcctg ctcttacaat ttttttgcga tggcggagct
     7681 gttgcaccgc ctggcaaagg gtgaaaaggg gacgccagaa ttatccctgc gcgacgatcc
     7741 ggcacaggaa accctgcgtt tcagcgccga tgccagcctt gccttcccgt gcagtgatat
     7801 cagtgcgctg aaacgggata cgtcaggggc attcaggatg accaccacct ttatggggtt
     7861 acaggggagc cagtcgcctc tccccggtta ttacctcgct cacctggcct ggaaagcggt
     7921 acatgaacaa agtccggtag gtgattttct ggatatgttc agccaccgcc tgacccagtt
     7981 tgtctggcat atctggcgta agtaccgcta tcacatcagt tttcgtaacg gcggtgtgga
     8041 tgcgttctcg cagcgcatgt actcccttgt cgggctgggc caccgccagc ttcgcgataa
     8101 gctggcgatt aatcacagca aaatgctggc gtattccggg atcctggcaa atccgggacg
     8161 atccccggaa attatctgtg gcctggtgtc ccactgcttt gatttgtcag aagtgacact
     8221 gcaaaactgg caacgtcgca aggtggatat tgaaccggat cagcaaaata gtctggggag
     8281 ctattctctt aaaaatggcg aaaaactggc gggaagatcg gtactgggca actttgttct
     8341 gggtacacga gtgcccgatc tcagcggaaa attccagctc agtattacca gcctgacccg
     8401 aaaacagttc ctctcttttc tgccgtccgg cgaaaacttc ctgccactga cgatgttcgt
     8461 gtccttcatc ctgcgcgatc agcttgcctg ggacttacat ctggggctgg cgccggagca
     8521 ggtgggcgca atgcgcctgg gcgataacaa aagtgcgctg ctgggctgga ccagcttcct
     8581 cggcacaccg gaagaacgac cctcagtcac catcagggta cggtcgtaaa ttatgataat
     8641 cacggagcag aatatgccgg aagagaaact acagacgctt tcattgcagg tcattaacgg
     8701 cagtgagctg gaaagcgggc gggcggcgcg ctgtctgttc acacagcagg gaaatgtcgg
     8761 ccatggcccc gaatgccact ggtcggtaca ggatcgtcag cagagcattc cggcccaggc
     8821 ttttaccgtt atcctgcacg atggcacatt ttgtctgcgc ccgcagacgg cacaactgtg
     8881 gctgaatcag gcaaaagtca cagcaacatc agacctaata cagttgcgcc agggcgatga
     8941 gatccagatc ggacggctga tggtgagggt tcatctgaac cggggagata ttccccatta
     9001 cgatgaggaa atggccactc ccgaaaccat cgttaccaat cgcgatatgc tcacggatac
     9061 cctgctatca acggagggtg cgccacacta tccgggaatg actcaccggc accagcttgc
     9121 agacaccgtg gtaaacggtt tttctgccga tccactccag gctcttcagt ccgaaagcct
     9181 gattaccacg ggcgatccgc tttcaggcat tgcggctgtc cggccatcgg caccgctgtc
     9241 cgatccggca agtaatgggg ggatcaatac tccgtttatg gatctgccgc ccatttatgc
     9301 cagccccggc gatcgtaatg acgacgtctc tgcggcagaa atggcgcaac gccacgttgc
     9361 ggtcaccccc ttactgcgcg gtcttggcgg ctcgcttacc atgagcaatt ccgacgatgc
     9421 ggatgatttt ctggaggagg ccggacgaac gttacaggcc gcaataaaag gtctgctcga
     9481 tttgcagcag cagcgtaaca gcctctcaga caaacatttg cgcccgctgg aagataaccc
     9541 gctgcgcctg aacatggatt acgccaccgc gctcgacgta atgtttgccg aaggtaaaag
     9601 cccggtacat ctggcggctc ccgccgccgt cagtgaaagc ctgcgcaatg tccgccacca
     9661 cgaagaagct aaccgggcag cgattgtgga gtcgcttcgt gtcctgctgg atgctttctc
     9721 accacaaaat ctgctgcgcc gctttgtgca gtaccgccgc agccatgaac tgcgccagcc
     9781 gctggatgat gccggagcat ggcaaatgta cagccattat tacgaagaac tggcctccga
     9841 tcgccagcag gggtttgaga tgctgtttaa cgaggtctac gcccaggtct atgaccgggt
     9901 gcttcgtgaa aaacagcggg agccggaagc atgaacagga aggcgtttct ggcctgcgtg
     9961 ctgatgtgcg tcatattaac cggctgcgaa acggcgaaaa aaatcagtga ggtgatcaaa
    10021 aacccggata tccaggtcgg gagcctgaag gagcaacctt cggaaataac cgtcaccctg
    10081 ctgaccgaac cggacaccaa taccaatgcg gaaggtgaat cagccgccgt ggatgtgcaa
    10141 ctggtgtacc tgactgacga ctcaaaattg ctggcagccg actatgacca gatagccagc
    10201 acgccgctac cggatgtgct gggtaaaaac tatatcgacc accaggattt caacctgttg
    10261 ccggacacca tcaaaaccct gccgccggta aagctggatg agaaaacgca gtttattggt
    10321 gtcgtggcct atttctccga tgaccaggcc actgaatgga aacaaatcga gacggtggaa
    10381 ggaatcggcc accattaccg cctgctggtg catgtacgtc aaagcagcat cgaaatgaaa
    10441 aaagaggatg aataattatg gcaaccaccc gcaacaaggt gatgtggcag gaagggatgc
    10501 tgatgcgtcc acaccatttc cagcagcagc agcgttacaa cgactacctg gataaccagc
    10561 gtttccgggc catgaatgat ttatcctggg gatttaccga actcaccctc aacaatgaac
    10621 tgctggcgca gggtaagatc atgattgaca gcgcgtcagg cacactgccc gacggcaccg
    10681 tcttttctat ccccgaccag gacgcactgc ccgatccgct gcacccgcaa aattttccgg
    10741 acgaaagaag ccgcaatatc tacctcgctc tgccggtcgc cagtgatgtg agaaatgaaa
    10801 tcagcgacgg acggcgaatc gggcgttacc ggctgaatta tgccgatgtc cgggatttgc
    10861 attcagaaga aggtgacgcg cgaacattga cgctgggtca actgacgccg cgcattatga
    10921 gcggtgcaga agatatgagc gcctatatta cgctcccgct ttgccgtatc agcgatcgcc
    10981 atgccgatgg ctccctgacg ttggatgacg attttatccc ctcctgccag aatattcagg
    11041 tcagtaagaa actgcgtgtt tatctcaaag aggtacaggg ggccattggc ggacgggcaa
    11101 gcgatctggc aaaccgtatt ggctctccgg cgcagagcgg catcgcggat gtggcggaat
    11161 ttatgatgtt gcagttactt aaccgtaacc agacccggtt tacccatcac gcccgtcgat
    11221 cccagctcca cccggaagat ttctaccttg atcttgccgg gttgctgggt gaactgatga
    11281 cctttaccga gccgtcgcgc ctgccctgcc cgcttgatgt gtatgatcat catgacctga
    11341 ccaaaatatt taaaacgctg ttaccggaag tcaaacgggc gctgcatacc gtactgtcgc
    11401 caagagccgt caatctgccg ctgcatctgc gtgacggtat ctggcaggcc gatatccatg
    11461 acacagaact gctgcaatct gccacctttg tgctggccgt ggcggcaaat atgccggtcg
    11521 atcagatcca gcgccagttt atccagcagt cgaaaatttc ctcgccggaa aaaatccgca
    11581 atatggtcag tgtgcagatc ccctgtattc cattgcgtgc ccttatggtg gctccccgcc
    11641 agcttcctta ccattccggg ttcagctatt tcgaactcga caagagtggc caggcctgga
    11701 cagaaatggc tgccgccggg gccgttgcac tgcatgtttc cggcagtttc ccggatctga
    11761 acatgcaact gtgggcgata agagggtaag acgatggatg aggggtcact gtctctgcca
    11821 ccatttaccg gacacgacga aaaaagccag cgcaactacc atctcgcgct tcgtggcaac
    11881 agcctgaatc cgatgatcga cgcggccacg ccgctactcg gtatggtaat gcgcctttca
    11941 accatgaaca gccagacgat gccggagcat ctttttgctc aggtggtgac agatgttcag
    12001 gccgtggagc aactgcttca ggagcagggc tacgaaccgg gcgtgattat ctcgtttcgc
    12061 tacatcctct gcacgtttat tgatgaagcg gcgctcggta acggctggtc aaacaagaac
    12121 gagtggatca agcagtcatt gctggtccat ttccacaacg aagcctgggg tggcgagaaa
    12181 gttttcatcc tgctggagcg cctgatccgt gaacccaaac gttaccagga tctgctggaa
    12241 tttctctgga tctgtttttc tctgggattt cgcgggcgct acaaagtggc agcacaggat
    12301 cagggggaat ttgagcagat ctaccgccgt ctgtaccacg ttctgcataa actgcgcggc
    12361 gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc agggcgggcg ttatcagctc
    12421 atcagtcgcc tgactgttaa acatattttt tgtggtggcg ttgtcgtgct ggcgttgttt
    12481 tacctgttct atctgttgcg gctggacagc cagacgcagg acatcctgca ccagctaaac
    12541 aaattacttc gataaggacg tttatgatcc agattgatct tcccacgctg gtaaaacggc
    12601 tgaacctgtt ctcccgccag gcgctggaga tggccgcctc tgaatgtatg agtcagcagg
    12661 cagcggaaat taccgtcagc catgtgctca ttcagatgct cgccatgcca cgcagtgacc
    12721 tgcgggttat tacccgccag ggcgatattg gcatggaaga gttgcgccag gcgctgacgg
    12781 tagagaacta cacaaccgcc cgttctgcgg acagctaccc ggcgttttcc ccgatgctgg
    12841 ttgagtggct taaagagggc tggctgctgg cgtcggctga gatgcagcac agcgaactgc
    12901 gcggcggcgt gttgctgctg gccctgctgc attcgccgct gcgttatata ccgcctgctg
    12961 ccgcccggct gttgaccggc attaaccgtg accgtctgca acaggacttt gtgcagtgga
    13021 cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg taaaggcgca ggcacaatga
    13081 cggacgcctc tgacaccctg cttgcccgct atgccaaaaa catgaccgca gacgcccgta
    13141 acggcaggct tgacccggta ctgtgccgcg accacgaaat cgacctgatg atcgacattc
    13201 tctgccgccg ccgtaaaaac aacccggtgg tggtgggcga agcgggcgtg ggcaaaagcg
    13261 cactgattga agggctggcg ctgcgcatcg tggcaggcca ggtgccggac aagctgaaaa
    13321 acaccgatat catgaccctt gacttgggcg cattgcaggc cggggcgtcg gtgaagggtg
    13381 aattcgaaaa acgtttcaaa gggctgatgg cggaggtcat ttcctccccg gtgccggtca
    13441 ttctgtttat cgacgaagca cataccctga ttggcgcggg caaccagcag ggcgggctgg
    13501 atatctccaa cctgctcaaa ccggcgctgg cgcgcggcga gctgaaaacc atcgccgcca
    13561 ccacctggag cgagtacaaa aaatacttcg aaaaagatgc cgccctgtcg cgccgcttcc
    13621 agttggtgaa ggtcagcgaa cccaacgctg ccgaagccac cattattctg cgcggtctgt
    13681 cggcggtcta tgaacagtct cacggcgtgc tgattgatga tgacgccttg caggccgctg
    13741 cgacattaag cgagcgttat ctctccgggc gtcagttacc ggacaaagcg attgatgtgc
    13801 tggataccgc ctgcgcccgt gtggccatca acctgtcgtc gccgccgaag caaatctcgg
    13861 cgctgaccac tctgagccac cagcaggagg cggaaattcg ccagcttgag cgcgagcttc
    13921 gcatcggact gcgtaccgac acatcacgga tgaccgaggt gctggtgcag tatgatgaaa
    13981 cgctgacggc gctggatgaa ctggaagcgg cctggcacca gcagcagacg ctggtccggg
    14041 agattatcgc gctgcgccag cagttactgg gcgtggcaga ggacgatgcg gcgccgttgc
    14101 cggacgcaga taccgtggag gatacgcagc cagagtcaga gtcagagtca gagtcagagt
    14161 cagagcagga taataccggt gccgaaccgg ctgatgaggc cgacagagaa caaccggaag
    14221 agaccgctga aacagtttcc ccggtacagc ggctggccca gctcactgcc gaactggacg
    14281 ccctgcataa cgaccggttg ctggtctccc cgcacgtcga taaaaaacag attgcggcgg
    14341 tgattgccga atggaccggc gtgccgctca accgcctgtc gcagaatgaa atgtcggtca
    14401 tcaccgacct gacgaaatgg ctgggtgaca ccatcaaagg ccaggacctg gcgattgcca
    14461 gcctgcataa gcatctactg accgcacgcg ccgacctgcg tcgtccggga cgcccgctcg
    14521 gtgcgttcct gctggctggc cccagcggtg tgggtaaaac cgaaaccgtc ctgcaactgg
    14581 cagagctgct ctacggcggt cgccagtacc tgaccaccat caatatgtcc gaattccagg
    14641 agaaacacac cgtctcgcgg ctgattggtt cgcctccggg ctacgttggc tacggtgaag
    14701 gcggcgtact gaccgaagcg attcgccaga aaccctactc ggtagtactg ctcgatgaag
    14761 tggaaaaagc gcacccggat gtgctcaacc tgttctacca ggcgttcgat aagggcgaaa
    14821 tggcagacgg tgaaggccgc ctgattgact gcaaaaatat cgtcttcttc ctgacgtcca
    14881 acctcggcta ccaggtaata gtcgagcatg ccgatgaccc ggaaaccatg caggaagtac
    14941 tgtatccggt gctggccgac ttcttcaaac ctgccctgct ggcgcgtatg gaagtggtgc
    15001 cgtatctgcc gctgtcgaaa gagacgctcg ccaccattat cgccggaaaa ctggcccgtc
    15061 tggataacgt gctgcgcagt cgctttggtg tagaagtggt cattgaaccg gaagtgacgg
    15121 acgaaatcat gagccgcgtc acccgcgcgg aaaacggcgc gaggatgctg gaatcggtca
    15181 tcgatggcga catgctaccg ccgctctcgc tgctgctgtt gcagaaaatg gcggctaaca
    15241 cggcgattgc ccggattcgg ttgtcggcag tggacggcgc atttacggca gacgtggaag
    15301 atgctcagaa cgacgagtcc gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt
    15361 tttccggcag acaggatggt ttatcgcggg cctgatgacg ggcttacccg ccactgccgc
    15421 gcccgcagaa gcatcgtcaa tggccggtgt ggcggtcgcc gtcgccacca cgaccccgcc
    15481 ggatgcgaca gccacgttac aggccatgca gtcctgtcgg cgggagtccg cggcgctgga
    15541 gcggcttgac tgctatgacc gcctgctggc accgctgtcc ccgtcagggt ttgacggggc
    15601 gctggtcaaa gccggttttg tgggcgaggc ctggacccgc gccacagagc aggaaaaacg
    15661 ccgcgagggc aacaccacag aactgctggt gacgcaggtg ccgggggagc gcccgacggt
    15721 ggtgataacc accccggcta tcggtcatgt tccgccacgc ccggtgctga tgttcagttg
    15781 cgtggacaat atcacccgga tgcaggtggc gctgatgcac cctctggatg tccatgatat
    15841 cgccgtcact ctgaatgccg acaaccgggc attgcgcagc cactggtttg tgcgcgagaa
    15901 cggcaccctg ctggagtcca gccgggggtt atccgggatt gatgaaatta agcaactgtt
    15961 cggcgcaaaa acgctgacgg tggataccgg cgcagacaat gccgccggaa agctgacctt
    16021 taacattgac gggctggcaa gggcgattgc tcccctgcgc gatgcctgcc actgggcggg
    16081 agaataaaaa tatggcaatg gacttacgcg atccgaatgt ctggatatcg cacctgctgg
    16141 aaaacctgcc ggaagagaaa ctggcatcgg cgctgaaaga tgacaacccg aactgggagt
    16201 atatcgacgg cgaaatcgtc aagctgggat cgctggccca cagtcaactg gatatcccgg
    16261 aactccagcg ccgggggctg gtcattctgg cttcggagag taaggatttt cgtctgctgg
    16321 cgcatctgct aagaaccctg caacacgccg gtgatcctca tctcgcattg cgcctgctgg
    16381 cgctgtatgt ggagcattac tgggcggtgg ctgcgccgca gaatatggcg cataaaaaac
    16441 gctttgccag ccaggtcatt aaacgctttg aaacaggtat tgaagttttt tcacagaacg
    16501 ccgccacagc gcagcgcgat gcgctgtcag ggcaactggc aaaactggcg caatgctggc
    16561 agtcacataa cgccccggaa ctggcacagg ctactgatga tctttttgcc ctgtaccagc
    16621 gtgcgtttaa tcgtgcggca ccggctcctg tccccactcc ggcggcctcc ggtagttcac
    16681 cacagaccac cgccacgtct gaaagcggcg tgacgcaacc cagtgctccg gctccccaaa
    16741 tcgtcatcga cagtcacgac gacaaagcct ggcgcgacac gctgttaaaa gtggcggcca
    16801 ttttatgtga acgccagccg gattcaccgc agggctaccg tctgcgccgc catgcgcttt
    16861 ggcagaacat caccagtacg ccccaggcgg aaagcgatgg ccgtactcca ctggctgcgg
    16921 tctccgccga tatggtggca gattaccatg ctcagctcgg tagtgccgat atggcgctgt
    16981 ggcagcaggt ggagaaaagc gtactgctgg ctccttactg gctggatggt cactgccttt
    17041 ctgcacagac ggcgctgcgt ctgggttaca aacaggtggc agacgccatc cgcgatgagg
    17101 tcatccgctt ccttgagcgt ctgccccagc ttaccgggct gctgtttaat gaccacacac
    17161 cgtttatcag tgagcagacg aaacaatggc tggcagcttc gcccgacgcc aaagttgcac
    17221 cggttgcgca aatcggtgag gaatcgaagg cagccagagc ctgttttgct gagcagggtc
    17281 tggaggcggc gctgcgatat ctggacatgc tacccgaagg cgatccccgc gatcagtttc
    17341 accgccagta ccttgccgca cagttgacgg aagaggcggg gctggtacag cttgcgcagc
    17401 agcagtaccg gatgttgttc aggatgggat tgcagatgat ggtggcagac tgggagccgt
    17461 ccctgcttga gcagctagaa caaaaattca cggcagaaca ataaaagaga acaggagttt
    17521 ttgtgttcag attaccaaca ccccgactac tcagcggact caaatcagcc ctgcgaccgg
    17581 cgatgccccg gtttaaagtc tctgctttct ggctgctgat actggcgtgg atttttctgc
    17641 tggtgtggat ctggtggaaa ggcccgatgt ggacgctgta tgaagaacag tggctcaaac
    17701 cactggcgaa ccgctggctg gcaacggcgg cgtgggggat tattgccctg gtgtggctta
    17761 ccgtccgggt gatgaagcgc ctgcaacagc ttgaaaaaat gcaaaagcaa cagcgcgagg
    17821 aagccgttga tccgctcagc gtggaactga acgcccagca gcgttatctt gaccgctggc
    17881 tgctgcgcct gcaacgccat ctcgacaacc gccgtttcct gtggcagttg ccgtggtata
    17941 tggtcatcgg cccggcgggc agtggcaaaa cgacactgct gcgcaagggg tttccgtccg
    18001 acattattta tgccccggag ggcgcacgcg gcgcagaaca acgcctgtac ctcacgcccc
    18061 atgtcggtaa acaggcggtg atctttgata tcgacggcac actctgcgct cccgctgatg
    18121 cggatatcct gcatcgccgt ctgtgggaac atgccctcgg ctggctgaaa gaaaagcgcg
    18181 cgcgccagcc gctgaacggg attattctga cactcgattt acccgatctg ctgaccgcag
    18241 acaaacgccg ccgcgagcat ctgttacaga cgctgcgcag ccgcttacag gatatacgcc
    18301 agcatcttca ctgccagtta ccggtttacg tggtactgac ccggcttgat ttattacagg
    18361 gttttgccgc cctgttccag tcactcaaca gacaggatcg cgatgcgatt ctgggcgtca
    18421 cgttcacccg ccgtgcccat gaaaatgatg actggcgaac agagttgaat gctttctggc
    18481 agacatgggt ggatcgaatg aatctggcgt tgccggatct gatggtcgct cagactcaca
    18541 cccgcgcgtc tttattcagt ttttcccgcc agatgcaggg aagccgtgaa ccgctggtgt
    18601 cactgcttga gggtctgctt gatggcgaaa atatgaacgt gatgctgcgt ggtgtctatc
    18661 tcacctcttc gcttcagcgt ggacagatgg atgatatatt cacccagtct gccgcccgcc
    18721 agtaccggct gggcaataac ccactggcgt cctggcccct ggtggacacc gcgccttatt
    18781 tcacccgcag cctgttcccg caggcattac tcgcagagcc taatctggca acagagagcc
    18841 gcgcctggct gatacgttcc cgtcgccgcc tgacggtttt ctctgccaca ggcggcgtgg
    18901 cagcactgct gctcatcacc ggctggcatc actattacaa cggtaactat cagtccggca
    18961 tcaccgtgct taagcaggcc aaagccttta tggacgtgcc gcctccgcag ggggaagatg
    19021 actttggcaa cctgcaactg ccgctcctga acccggtacg cgatgccaca ctggcctatg
    19081 gcgactgggg cgaccgcagc cgtctggccg atatgggact gtaccaggga cgacgtatcg
    19141 ggccttatgt ggaacagacc tatctgcaac tgctggagca acgttacctg ccctcgctgt
    19201 ttaacgggct ggtcaaagcg atgaacgccg cgccgccgga gagtgaagaa aaactcgcgg
    19261 tgctgcgcgt gatgcgaatg ctggaggaca aaagcggacg taacaatcag gtggtgaagc
    19321 agtatatggc aaaacgctgg agcgaaaagt tccacggtca gcgcgatatc caggcacaac
    19381 tgatgtccca tcttgactac gcgctggctc atactgactg gcacgcagag cgtcaggcgg
    19441 gcgacggtga cgccatcagc cgctggacgc catatgacaa gcccgtggta tcagcacaga
    19501 aagaactgag caaactgcct gtctaccagc gggtttacca gagcctgaaa acgcgggcgc
    19561 tgggcgttct tcctgccgac ctcaatctgc gtgaccaggt agggccaacc tttgaccagg
    19621 tgtttacatc tgccgatgac aacaaactgg ttgttccaca gtttatcacc cgctacggcc
    19681 tgcaaagcta ttttgtaaaa cagcgcgatg aactggttga actgacagcg atggattcct
    19741 gggtacttaa cctcacccgc aatgtgaaat acagtgacgc cgaccgtgcg gaaatccagc
    19801 accagttgac cgagcagtat atcagcgact acaccgccac ctggcgggct gggatggaca
    19861 atctgaatat ccgcaatttt gagtccatcg gacaactgac cggggcgctg gagcaggtta
    19921 tcagcggcga ccagcctttg cagcgggcgc tgaccgtgct gcgtgacagc acacagccag
    19981 gcgtcttttc tgaaaaactc tctgccaaag aacgggagga agccctggca gagccggatt
    20041 accagttact cacccgcctc gggcatgaat tcgccccgga aaacagtacc ctggcagtac
    20101 agaaagacaa agaaagcacg atgcaggccg tgtatcagca actcaccgag ttgcaccgct
    20161 acctgctggc aatccagaac gcgcctgtac cagggaaatc ggcgctgaaa gccgtgcagt
    20221 tacggcttga tcagaacagc agcgatccga tattcgccac ccgccagatg gcaaaaacgc
    20281 tgcctgctcc gctcaaccgc tgggttggca gactgactga ctaggcctgg catgtggtga
    20341 tggtggaggc tgttcattat atggaagtgg actggcgcga cagcgtggtg aaaccgttta
    20401 acgagcaact ggcaaataac tatccgttta atccgcgttc tgcacaggat gcctcactgg
    20461 atgccttcga acgcttcttt aaaccggatg gcatactgga taccttctac cagcagaacc
    20521 tgaagctgtt tatcgataat gacctgagtc tggaggatgg cgataacaac gtcattattc
    20581 gcgaagatat tattgcgcaa ctggaaactg cgcagaaaat ccgtgacatc ttcttcagca
    20641 aacagaacgg tctgggaaca tcctttgccg tggaaacggt atcgctttca ggcaataaac
    20701 gccgcagtgt actgaacctt gacagtcagt tagtcgatta cagccagggc cgtaactata
    20761 ccgcccatct ggtctggcct aacaacatgc gcgaaggcaa cgaaagtaag ctgacgctca
    20821 tcggcaccag cggcaacgcg ccgcgcagta tcagcttcag cgggccgtgg gcgcagttcc
    20881 gcctgttctg ggccggacaa ctgaccggag tacaggatgg caactttacc gtgcgcttta
    20941 gcgtggacgg tggcgcgatg acctaccgtg tgcataccga cacggaagat aacccgttca
    21001 gcggtgggtt gttcagccag tttggtctgt cagacacact gtactgagga ggcagggatg
    21061 aatagtaacg tactgacaca aactatcgtt accggcagtg acccgcgcgg gctgccggaa
    21121 ttcagcgcca tccgcgagga aataaacaaa gccagccacc cgtcacagcc tgagctgaac
    21181 tggaaactgg tggagtcgct ggcgctggcg atttttaaag ccaacggtgt ggatttacac
    21241 accgccacct actatacgct tgcccggaca cggacacagg gactggcggg attctgcgaa
    21301 ggtgcggaac tgctggcggc aatggtaagc cacgactggg ataagttctg gccgcagggc
    21361 ggcccggcgc gtactgaaat gctggactgg tttaactccc gaaccggcaa tattctgcgt
    21421 cagcaaatct cctttgcgga atccgaccta ccactgatct accgcacaga gcgggcattg
    21481 cagcttatct gcgataagct ccagcaggtg gaactgaagc gcgttccgcg cgtggagaat
    21541 ctgctctatt ttatgcagca cacgcgtaaa cggcttgaac cccagccgaa gagtaacgct
    21601 gagaacgccg cacagaccac ggtcagaacg ctgatttatg ccccggaaac actggcatct
    21661 tccacaccag aagcggtagt gcctcccctg cccggcctgc ctgagatgaa agtggaagtg
    21721 cgcagtctga cagagaatcc cccacaggcc agtgtgataa agcaaggcag tacggtaaga
    21781 gggtttatcg cagggatcgc ctgttcagtg gctgtcgcct cagcattgtg gtggtggcag
    21841 gtctatccgg tgcagcagca actgttacag gttaacgaca ccgctcaggg cgcagcaacg
    21901 gtgtggatgg cctcacctga actcgaaaac tatgagcgca ggctgcaaca acttcttgat
    21961 acctccccgg tacagccgct ggaaaccggg atgcagatga tgcgtgttgc cgacagtcgc
    22021 tggccggaaa gcctgcaaca gcaacaggcc tcgacacaat ggaatgaggc actcaaaacc
    22081 cgcgcacaga gtagcccgca gttgcgtggc tggttgcaga cccgccagga cttacatgct
    22141 tttgcagatc tagtgatgca gcgcgagaaa gagggactaa ccctttccta tatcaaaaat
    22201 gtcatctggc aggcggagcg gggactgggg caggaaaccc ccgttgagtc tctgttgacg
    22261 cagtaccatg atgcccgtgc gcagaagcag aatacagatg cgctggaaaa acaaattaat
    22321 gagcgactcg aaggcgtgtt aagccgctgg ctgctgctga agaataacgt catgccagag
    22381 gcggcaaccg gtaccacggc cgaaaaataa ttatttaatt aaaggagatt atcatggcaa
    22441 atataagtta tttatctttg agtggagaga cacaagggct aatttcagca ggttgctcaa
    22501 cgcttgattc tgtcggaaac aaagcgcagc cagaacataa agatcagatc atggtttatg
    22561 cgttaatgca ttccatctca agaagtcaaa atgtcaatca tcatgaatta attatcacca
    22621 aaccggttga taaatcctca ccattgctgg caaaagcact tagtgacaat gaaaaaatgg
    22681 caatatgtga atttatactc taccgaacca gtaaagcggg tatttaccag ccttattata
    22741 aaataaatct gtcaaaagcc cgaatatcaa gtattgattt tgtcacaccg catgctgtcc
    22801 tggaaaaaga actggaacca caggagagaa ttgcctttat ttatgaagat atttcctggg
    22861 agcatacgtt agccggtacg aatgcaatga gtaaatggca ggatcgtgta cagtaactta
    22921 ctgtaataac ataataaatt actattgcgg agagataaaa tggctggttt aaggaataac
    22981 aacaacacac agaatgcaca gtgggctgat tatgtgggtg acattcttcg gggggctcag
    23041 ccgataaacc agctagtacc tcagcaccct tatcttaacg acgtcccact cattgatgag
    23101 ttaaggcatc agaacactca tcatgttatt ctcttgacac ttgatgttgc aaaaaaaaaa
    23161 aaaaaaaaaa atactcagcc caataacatc atttgattac attcatttta tcactacaca
    23221 tccttcaggt ataaaagata cactcgcctg gttagttaac gcgagtaaat taatgactga
    23281 gtttgatgac aatgggaaaa ttatatttaa cattaatgcc ttaaagtata caaaagcaag
    23341 ttactttgaa atacttggag agaaatatat aaaaatcaca acatccagcc cctggctttt
    23401 agaaaagctc ggtaaatata ttttctcttc acgagcgcct caagttctgg aactcgctat
    23461 tggctggcga ggagctttat ctgaatcaat aaaaggagtt aagttctgta tctggttttc
    23521 agtagcctgg agaaccatag aatttattat gagttcagag cgagatctgg ttaattttct
    23581 tggcgatttt tcaatggatg tggccaaagc agttatagcc ggtggtgttg caaccgctat
    23641 tggaagcctg gcttcttttg cctgtgttag ctttggcttt ccagtaattc ttgtcggagg
    23701 agcaatttta ctgacaggga tagtgtgtac agttgtttta aatgaaatcg atgctcaatg
    23761 ccatttatca gaaaaattaa aatatgcaat tagagatgga ctaaaacggc aacaggaact
    23821 tgataaatgg aaaagggaaa acatgactcc atttatgtat gttcttaaca ctccacccgt
    23881 gatatgagga ttaaccatgc gtttacatgt taaattaaaa gagtttcttt cgatgttttt
    23941 tatggccata ttatttttcc ctgcattcaa tgcttctttg ttttttaccg gagttaaacc
    24001 tctatattca ataataaaat gctccacgga aatattttat gattggagaa tgttgattct
    24061 gtgctttggt ttcatgtcgt tttccttttt aaatattcat gtaattttac ttacgataat
    24121 aaaattattc ttaatcaaaa aaacgaaggt agtcaatttc gccacagaca taacaataca
    24181 gctaactctt atatttttac taatagctat cgtaatagca cctttaatag ctccttttgt
    24241 cacaggatat gtcaatgcta attatcaccc ctgcggtaat aatacgggaa ttttcccagg
    24301 agcaatctat attaaaaatg gcatgaaatg taataatgaa tatatttctc gcaaggaaga
    24361 ttcagcagtt aaataattaa acattctaaa acaaacagtt tttaactccg gattccatgt
    24421 cctttcttgc actgtctttg tcaagaaaaa tttcttatgc aaagaatttt gtattatttc
    24481 cgccagaaat tcctggcggc agatataatt tattattgat agcggttaac caaatctttt
    24541 accatcgctt tataggtctt ttgcttaagt tgcatgattt gctgctctgt catatcagtc
    24601 gtactggctt cattcttgtc gggcaatggt ggaagcactt tatattcata attcaccggc
    24661 gaccaggcag cccactcacc tgtagctaca tccaccagcg tgaatcgtac cagatagcgc
    24721 agtgacatcg tgtcagtcaa tttttgattt ttataatctg accaaactat tgatttcaat
    24781 ccagagtcat attttcctgt ctgcaacgta tcctggaaaa caataatcgc tttctgatgc
    24841 ccttttgcag ccacaaaacg cagtgcctgc atccagttca tattctcagc actggcaaca
    24901 tcctcgttct catttttatc tttgtttttg ttacaagtca gaggcttttg cctgtccggg
    24961 ataccagaga atgtggaaac agtataatat tttcgcatct cctcctgcat aatggtttcc
    25021 ggtgcgcggt tgccggattg aacaagtatt acaggagaat taaggggaac gcggaactca
    25081 tctccatcaa gcgcagcttg aatatcctct tcggatacgg ccagagtggt ttcattaccg
    25141 aataaatctt tgtctgtcag ttgctgggga gtgtttttat taatattact ttgtgtgact
    25201 tgagcttttt tcttcttttc atcaatagaa gattcagagg cacatcctgc aagcagcgag
    25261 aataatacac atatacaaca aacccttctt aaattcataa tcggacactc cctcgcctgt
    25321 cataatcatg gtaatgttta gctaacgatt tttaattata tactccactt gaatcagaac
    25381 aaacaaaaat aacagtatat ataaattcat taaatcaata gaaaaatgaa aatcataaca
    25441 ttcaaatata tttaaattga atgattcgat agtatgatta aatattaatg atatggtcta
    25501 ccatatgtga ttaacacatt tacagcataa caaaaccata gaccgaagaa atagcgggat
    25561 ttaaatgcaa aaaaaccgca aaattaaaaa tcttgcggtt ctctgaactc attttcatga
    25621 gtgaatttgg cggaacggac gggactcgaa cccgcgcccc cctgcgtgac aggcaggtat
    25681 tctaaccgac tgaactaccg ctccgcgttg tgttccgttg ggaacgaggc gaatattacg
    25741 gattgcctca cccttcgtca acggtttttc tcatcttttg aatcgtttgc tgcaaaaatc
    25801 gcccaagccg ctatttttag cgccttttac aggtatttat gcccgccaga ggcagcttcc
    25861 gcccttcttc tccaccagat caagacgggc ttcatgagct gcaagctctt catctgtcgc
    25921 aaaaacaacg cgtaacttac ttgcctgacg tacaatgcgc tgaattgttg cttcaccttg
    25981 ttgctgctgt gtctctcctt ccatcgcaaa agccatcgac gtttgaccac cggtcatcgc
    26041 cagataaact tccgcaagga tctgtgcatc gagtaatgcc ccgtgtagcg ttcgtttact
    26101 gttatctatt tcgtagcggg cacataacgc atcgaggctg ttgcgcttac caggaaacat
    26161 tttcctcgcc accgcaaggc tatcggtgac cttacagaag gtattggtct tcggaatatc
    26221 gcgcttaagc aacgaaaact cgtagtccat aaagccgata tcgaacgctg cgttatggat
    26281 caccaactcc gcgccgcgga tatagtccat gaactcatcg gctacttcgg caaacgtggg
    26341 cttatcgagc aaaaattcat cggcaatacc atgtacgcca aaggcttccg gatccaccag
    26401 ccgatcgggt ttgagataaa catggaagtt attgcccgtc aggcgacggt tcaccacttc
    26461 aacggcacca atctcaatga tcttgtggcc ttcatagtgc gcaccaatct ggttcatacc
    26521 ggtggtttcg gtatcgagaa cgatctggcg tgtaattgca gtgctcatag cggtcattta
    26581 tgtcagactt atcgttttac agttcgattc aattacagga agtctaccag agatgcttaa
    26641 acaggtagaa attttcaccg atggttcgtg tctgggcaat ccaggacctg ggggttacgg
    26701 cgctatttta cgctatcgcg gacgcgagaa aacctttagc gcaggctaca cccgcaccac
    26761 caacaaccgt atggagttga tggccgctat tgtcgcgctg gaggcgttaa aagaacattg
    26821 cgaagtcatt ttgagtaccg acagccagta tgtccgccag ggtatcaccc agtggatcca
    26881 taactggaaa aaacgtggct ggaaaaccgc agacaaaaaa ccagtaaaaa atgtcgatct
    26941 ctggcaacgt cttgatgctg ccctagggca acatcaaatc aaatgggaat gggttaaagg
    27001 ccatgccgga cacccggaaa acgaacgctg tgatgaactg gctcgtgctg cggcaatgaa
    27061 tcccacactg gaagatacgg gctaccaagt cgaagtttaa gcttgtggtt tacgacattg
    27121 ccgggtggct ccaaccgcct ggcgaattcg tggcttgttt ttactctgtt tcatcgggtt
    27181 tagcgttaaa ggaatagtcc gtttccgggc aacaataagt tgtaagcaac caagcgcagg
    27241 aatatgcgca ttcaatagtt ttcctccgtg tttgttccac gggagaacgt ggaaacggct
    27301 ggcgtgcagc acttcaaaat tcaacaaaga gagccagtcc agttgccgca tcagagtaaa
    27361 catccggctg ttatagggcg aggtttttcg caataccggc acaagtttgc gtaatcccat
    27421 taaactaatg ggattgaagc cactaatcac cagccagcca tcatcaatca atacccgatc
    27481 ggtttcacgc aataaacgat gcggatcggt gcaccacggc aatgtatgtg ccagtagaca
    27541 aacatcaacg gatttatcgg caaaaggaag atgaagtgga tccgcctgca cctggacggg
    27601 cattccttgc gcagaaacat tcacttgatg agaaaccgcg cacgcttcgc aattgatttc
    27661 tgcgcttaaa ttgccaatct taagcagatg aaaaccatac attttagtga accacgggtt
    27721 gagctggcgc tccagcgcct tgcgataaag ctctccccag ggcaaatcgc cccagcgatc
    27781 aggagccacg acagtttgag ggactcttgc cggtttcatc acaaccttcc gtttcacact
    27841 aagaggtaat ctatgaatct taacagtatt cccgcctttg atgacaatta catctgggtt
    27901 ttgaatgatg aagcaggtcg ctgcctgatt gtcgatcccg gagacgcaga gccagtatta
    27961 aacgccattg ccgccaataa ctggcaaccg gaggccatat ttctcaccca ccatcatcac
    28021 gatcacgttg gcggcgtaaa agaactggtg gaaaagtttc cgcaaattgt ggtgtatggt
    28081 ccacaagaga cacaagataa gggaacaaca caggtagtca aagatggcga aactgccttc
    28141 gttttggggc atgaatttag tgtaattgcc acaccgggtc acactttagg acatatctgt
    28201 tacttcagta aaccttatct attttgcggc gacacgctgt tttctggtgg gtgtggtagg
    28261 ttgtttgaag ggacagcatc acaaatgtat caatcactta ataagttaag tgcgttacct
    28321 gacgatacat tggtatgttg tgctcatgaa tatacgttat caaatatgaa gtttgctttg
    28381 agtattcttc cgcacgattt gtccataaat gattattatc gtaaagttaa ggagttacgg
    28441 gcaaaaaatc aaataacact acccgtaatt ctgaaaaatg agcggcaaat taatgttttt
    28501 ttaagaacgg aagatattga tttaattaat gtaattaatg aagaaacatt attgcaacaa
    28561 cctgaagagc gttttgcatg gttaaggtca aagaaagata ggttctgata aaactttctt
    28621 gtcatcggct ccgttcgccg ttatgatcgg tcgtctttta agcaactatt gacacacaca
    28681 tgaaggcaaa agcgatatta ctcgcctctg tcctgctcgt gggttgccag agtaccggca
    28741 acgttcaaca gcacgcacag agcctttctg cagctggtca aggggaagca gcaaagttta
    28801 caagtcaggc acgatggatg gacgatggga cgtctatcgc gccagatggt gacttgtggg
    28861 ctttcattgg cgacgagcta aagatgggaa ttccggaaaa tgaccggatt cgcgaacaga
    28921 aacagaaata tttacgcaat aagagctatc tccacgatgt aactttacgg gcagagccgt
    28981 atatgtactg gatagcaggg caagttaaaa aacgtaacat gcctatggaa ctggtactac
    29041 tacccatagt ggagagcgct tttgatcctc acgcaacgtc tggcgccaat gccgcaggca
    29101 tctggcagat cattccgagc acggggcgca attatggttt gaaacagacc cgcaattatg
    29161 acgcgcgtcg cgatgttgtt gcttcaacaa ctgccgcgct gaatatgatg cagcgtctga
    29221 acaaaatgtt tgacggcgac tggcttctga ccgtagcggc ttataacagc ggcgaaggtc
    29281 gggtcatgaa ggcaattaaa acgaacaaag cgcgtgggaa atccacggac ttctggtcgt
    29341 taccgttgcc gcaggaaacg aagcagtacg tgcctaaaat gctggcattg agtgatattc
    29401 tcaaaaacag caagcgttat ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg
    29461 cgcgtgtgca cctgagtagc ccggttgaaa tggcgaaggt tgcagatatg gcggggattt
    29521 ccgtcagcaa gctgaagaca ttcaacgctg gcgtgaaagg ctccacgctg ggcgcaagtg
    29581 ggccgcagta cgtgatggtg ccaaagaagc atgcagatca actgcgtgaa tctctcgctt
    29641 caggcgaaat tgctgctgta cagtcgacgc tggttgccga caatacgccg cttaacagcc
    29701 gtgtttacac cgtacgctct ggcgacacgc tttcaagcat cgcttcacgt ctcggcgtaa
    29761 gcaccaaaga tttgcagcag tggaacaaac tgcgcggatc taagctgaag ccaggccaaa
    29821 gtttgacgat tggtgcaggc agtagcgcac agcggttggc aaacaacagc gatagcatta
    29881 cgtatcgtgt gcgcaaaggc gattcgcttt caagcattgc taaacgccac ggcgtgaaca
    29941 tcaaagatgt gatgcgctgg aacagcgaca ctgcgaatct gcaaccaggc gataagctga
    30001 cgttgtttgt gaaaaacaac aacatgccag attcctgaca aaccagataa taaaaaaggc
    30061 accgattccc ccggtgcctt ttttatttat gccgctttat gtgcttctac catgatgata
    30121 tcactggtga aagagccatc agtctgcaag gaaaaatacg ttctcacctc tgtcgatgcg
    30181 ctctgttggt aaatgcgaat agcgtctact aacgcttctg gcgtacgcat tctcacgacc
    30241 catgaagaaa attccagcgg taacttatct gtaattaaat tatcaactat cagattggct
    30301 tcattgatta acgtcaacca cgcaccgctg gcgtagtttc gtacgtgaga ggtatcgcgt
    30361 aatgcttcta ccgcctgtaa ccagatgtcg cgcactgggt gacccggaga cattacatcc
    30421 atcacaatca gcctaccgcc aggtttcaat atcctattca cttctcgcag tgctgcacca
    30481 acatcatgcc agtgatgggc agaataacgg ctgataacaa tatcaaatgc gttatcggca
    30541 aatggcagac ttgcggcata tccctggcgg gtggtgatat ttttcagttg ccgggcttcg
    30601 gcagcttgtg ccacgacatc cagcatgtgg gcagataagt catacgccac caccgcgctc
    30661 acgttttgcg cagcgccaaa cgttggtctg ccctgcggca gacctgacat accttattta
    30721 tcaggcttgc cgggactgaa ttcaacgagt agcggattgt gatcggaagc gcgcgtaacc
    30781 agtacagaag cttcactgac gttcagacca cggtagaaaa caaaatcgag cgggcgacca
    30841 aacgcccgac ggcgctgatc atcggtaaaa cgcacctggc gcagcgacat ttcccgcgca
    30901 aagcgatata acgcgttcat ccttctacgg ctccaggcat tgaaatctcc cgccataatg
    30961 accgggccgc tgtggtgagc tatctgatca ccaataggaa gtaactgctt actatagaca
    31021 tccacgccca gactgaagtt gacggcgtgt atattaacca ccatcaacag gcgggtgtca
    31081 ggtaatggat agaccgtcac cagtgccgac ttcgccagac gcaaaatggg ttctcgttca
    31141 cgtaacgggc agcaatacac tggatgtgcc gccgaaaggg tcattacgcc agaaggatgt
    31201 tgtggcagca cgaaagcggg tacctgatcg gcggcaagat agttagcggt cgcaaactgt
    31261 accaactctg gcgttgtttg cgcttcctgc aataacacca gatgtgcgtc tttgccgtaa
    31321 ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt atatgttcca caccaggatc
    31381 cgaatacgct cttcggtact taacggttct ccgggtggta atgcctggcc gatgctcgca
    31441 aaagaccccg gcggtaagat cctctccgca ggttgtccgg caacatagcg catggcatag
    31501 gtgttttttc gcacttttga ctttgaaacc tctattacgt caacccttcc gcactcggga
    31561 gggttctact gttataggga ttttagctca ctctttcaac gagcaattgt atattgttat
    31621 gtaagcaagt gcttacaccg gtcagaaatt tgctcattat tgagaacatt aaatcggatg
    31681 acatatcacg catcctgatg gtttaatgac aaataaattc aaaatatctc atctattttg
    31741 caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc catttttgtt tcggtcgtcg
    31801 aaagcggcag ctttagccgg gcagcggaac aattagggca agcaaactca gcggtaagcc
    31861 gggcggtgaa aaagctggaa atgaaacttg gcgttagcct gcttaatcgg accacgcggc
    31921 aacttagcct gacggaagaa ggcgagcgtt attttcgtcg cgtacagtca attttgcagg
    31981 agatggcagc ggcagaatca gaaattatgg agacgcgtaa tacaccgcgt ggactgttac
    32041 ggatcgatgc cgcaactcca gtggtgctgc actttctgat gccgttaatt aagcctttcc
    32101 gtgaacgcta tccggaagtc actttatcgc tagtctcctc cgaaacgatt attaatttga
    32161 tcgaaagaaa agtggatgtc gcgatacgcg ctggtacgtt aacggactcc agcttacgtg
    32221 ccaggccgtt atttaacagt tatcgaaaaa ttatcgcctc ccccgattat atttcccgct
    32281 acgggaagcc agaaacgatc gacgatttaa agcaacatgt ttgcctggga ttcactgaac
    32341 ccgcttccct caatacctgg ccgatagcct gtagtgatgg acaattacat gaggtgaagt
    32401 acggtttgtc atccaatagt ggggaaacac tgaaacagct ttgcctgagt ggcaacggga
    32461 ttgcgtgttt gtccgactac atgatcgaca aagaaatcgc tcgcggagaa ttggtggagt
    32521 taatggcaga taaagtgttg ccagtggaaa tgccattcag tgccgtctat tacagcgacc
    32581 gtgcggtaag tacgcgcatc cgggctttta tcgatttcct tagcgagcat gtaaaaacag
    32641 ctcccggagg agctgtcaga gaggcttaat cccattcagg agccagacct tccgggctaa
    32701 ccaggcggtc gttgcaatcc agtgcggcga tcgctttttt atcttcggca tcaagctgta
    32761 aattttgtgc cttaagatta ctttccaggt ttttacgttt agtagaagaa ggaattactg
    32821 agtaaccttc ccccatagcc cacgccagaa tcacttgtgc cggagtcgca ttgtgtttag
    32881 ctgcaatacg agcaataacc tcatctttca gtgccttacc ataagccagc gtcatatagg
    32941 aagtgatatg aataccgtgc tgtttagccc aggcaaccac tttacggttt tgcagataag
    33001 gagagagttc aatctggttg gtagcgatgt tttcagcgcc aacagcagca atcgcttttt
    33061 ccatcaacgg gatcgtgaag ttggaaatac cgatctcacg tgtcagacct tgttttttgg
    33121 cctccagcag cgcctgcata aactcttcaa cagagacttc atcgtttggt gacggccagt
    33181 ggattagcgt cagatcaaca taatcggtac gcaatttttg caggctctct tttagactcg
    33241 ggatcaattt gtctttgctg agattttcaa tccagatttt agtggtgatg tagagttcat
    33301 gacgtggcac gccactttct gcaatcgcct gacctactgc ggcttcgtta tcatagattt
    33361 gtgcagtatc aattgcgcga taaccaagtt caagcgccgt tttcacagat gaaataacaa
    33421 cgtcgtcttt gagacggaaa gtacctaaac caaatgcagg gatagccata atatgcctct
    33481 tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc agactgttgc ggaaaaagag
    33541 cataaaatgc agaggatttt tgcgattctg gcaataatag atatacagac gaaaaaaaac
    33601 cttgagcgtt aactcgaggc ttcttaataa gtggcggaac ggacgggact cgaacccgcg
    33661 accccctgcg tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt
    33721 tacaaccacc ggttttatga ccggcttact gcttaatttg atgcctggca gtttatgaat
    33781 cacttcgtga ttcacccttc gggccgttgc ttcgcaacgt tcaaaagctt tcgcttttgt
    33841 cctactctct tatcgagtag tgaactgcct caaaggtcag tgccattctt c
//
LOCUS       DAQSWQ010000041        32840 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.041, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000041 DAQSWQ010000000
VERSION     DAQSWQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.041"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            269..1927
                     /locus_tag="R8G18_001998"
     CDS             269..1927
                     /locus_tag="R8G18_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HEE9795698.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1924..2880)
                     /locus_tag="R8G18_001999"
     CDS             complement(1924..2880)
                     /locus_tag="R8G18_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEE9795699.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3031..4146
                     /gene="macA"
                     /locus_tag="R8G18_002000"
     CDS             3031..4146
                     /gene="macA"
                     /locus_tag="R8G18_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HEE9795700.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4143..6089
                     /gene="macB"
                     /locus_tag="R8G18_002001"
     CDS             4143..6089
                     /gene="macB"
                     /locus_tag="R8G18_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HEE9795701.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6162..6386)
                     /gene="cspD"
                     /locus_tag="R8G18_002002"
     CDS             complement(6162..6386)
                     /gene="cspD"
                     /locus_tag="R8G18_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HEE9795702.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6709..7029
                     /gene="clpS"
                     /locus_tag="R8G18_002003"
     CDS             6709..7029
                     /gene="clpS"
                     /locus_tag="R8G18_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEE9795703.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7060..9336
                     /gene="clpA"
                     /locus_tag="R8G18_002004"
     CDS             7060..9336
                     /gene="clpA"
                     /locus_tag="R8G18_002004"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEE9795704.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9742..9829)
                     /locus_tag="R8G18_002005"
     tRNA            complement(9742..9829)
                     /locus_tag="R8G18_002005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9793..9795),aa:Ser,seq:gga)
     gene            complement(10083..10301)
                     /gene="infA"
                     /locus_tag="R8G18_002006"
     CDS             complement(10083..10301)
                     /gene="infA"
                     /locus_tag="R8G18_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEE9795705.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10586..11290)
                     /gene="aat"
                     /locus_tag="R8G18_002007"
     CDS             complement(10586..11290)
                     /gene="aat"
                     /locus_tag="R8G18_002007"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEE9795706.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11332..13053)
                     /gene="cydC"
                     /locus_tag="R8G18_002008"
     CDS             complement(11332..13053)
                     /gene="cydC"
                     /locus_tag="R8G18_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HEE9795707.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13054..14820)
                     /gene="cydD"
                     /locus_tag="R8G18_002009"
     CDS             complement(13054..14820)
                     /gene="cydD"
                     /locus_tag="R8G18_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HEE9795708.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14943..15908)
                     /gene="trxB"
                     /locus_tag="R8G18_002010"
     CDS             complement(14943..15908)
                     /gene="trxB"
                     /locus_tag="R8G18_002010"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEE9795709.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16453..16947
                     /gene="lrp"
                     /locus_tag="R8G18_002011"
     CDS             16453..16947
                     /gene="lrp"
                     /locus_tag="R8G18_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HEE9795710.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17082..21149
                     /gene="ftsK"
                     /locus_tag="R8G18_002012"
     CDS             17082..21149
                     /gene="ftsK"
                     /locus_tag="R8G18_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEE9795711.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21304..21915
                     /gene="lolA"
                     /locus_tag="R8G18_002013"
     CDS             21304..21915
                     /gene="lolA"
                     /locus_tag="R8G18_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEE9795712.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21926..23269
                     /gene="rarA"
                     /locus_tag="R8G18_002014"
                     /gene_synonym="mgsA"
     CDS             21926..23269
                     /gene="rarA"
                     /locus_tag="R8G18_002014"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HEE9795713.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23360..24652
                     /gene="serS"
                     /locus_tag="R8G18_002015"
     CDS             23360..24652
                     /gene="serS"
                     /locus_tag="R8G18_002015"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEE9795714.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24891..27335
                     /gene="dmsA"
                     /locus_tag="R8G18_002016"
     CDS             24891..27335
                     /gene="dmsA"
                     /locus_tag="R8G18_002016"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HEE9795715.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27346..27963
                     /gene="dmsB"
                     /locus_tag="R8G18_002017"
     CDS             27346..27963
                     /gene="dmsB"
                     /locus_tag="R8G18_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HEE9795716.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27965..28828
                     /gene="dmsC"
                     /locus_tag="R8G18_002018"
     CDS             27965..28828
                     /gene="dmsC"
                     /locus_tag="R8G18_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HEE9795717.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28864..29490)
                     /locus_tag="R8G18_002019"
     CDS             complement(28864..29490)
                     /locus_tag="R8G18_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEE9795718.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29805..30953
                     /locus_tag="R8G18_002020"
     CDS             29805..30953
                     /locus_tag="R8G18_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9795719.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31163..32593
                     /locus_tag="R8G18_002021"
     CDS             31163..32593
                     /locus_tag="R8G18_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEE9795720.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32594..>32840)
                     /locus_tag="R8G18_002022"
     CDS             complement(32594..>32840)
                     /locus_tag="R8G18_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9795721.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8058 a         8033 c         8815 g         7934 t
ORIGIN      
        1 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcacgcaa aagatttaat
       61 cctttaggcg taataaaaaa taatatatca tgctaattat ttgattttgt tgtttttgca
      121 gacttatcag caagagggag tataacgcga ttattcgctc atttttcaga catttgccat
      181 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg ccagcgtaaa
      241 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      301 tcgcggtatc aaccgtttgt cgttgatgct ggaacaaaac aacgtcctga ttggggagaa
      361 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      421 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      481 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      541 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      601 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      661 cgataaagac ggacatccga ttgatgtcga ggatattaac gatcaggcac gccatctggt
      721 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      781 cacggtgcca aatgtcccta atatggaagt caccgcgcgc cagctcgatt tcctcgcccg
      841 tgagttatcc tcacatccgc aaaatctctc tgatgggcag attcgtcagg gactttccgc
      901 aatggtacag ctgcttgagc attatttctc tgagcagggg gccggacagg cgcgatatcg
      961 tttaatgcgg cggcgagcca gcaatgagca acgaagctgg cgctatctgg atatcatcaa
     1021 ccggatgatt gaccgacctg gtgggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1081 tactttgttg caggcaaaag gcacattgcg actggataaa gacgcccgtc cattgttgct
     1141 gatcgaagat ccagaaaccc gtttacaccc cattatgctt tcagttgcct ggcatctgtt
     1201 gaatcttctg ccattgcagc gcattgtcac caccaactcg ggtgagttgc tttcgttaac
     1261 gccggtagag catgtttgcc gactggtacg tgagtcctcg cgcgttgccg cctggcgtct
     1321 ggggccgagt ggcttgagta ccgaagatag ccgacgcata tcctttcaca ttcgttttaa
     1381 ccgtccgtca tcgctgtttg cacgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1441 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1501 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1561 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1621 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1681 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1741 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1801 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1861 cgtaccgacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     1921 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     1981 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2041 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgatt cccagaaagc
     2101 gttgtaatca gcgtggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2161 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2221 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2281 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2341 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2401 aaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2461 gaactgctcg ccgtttttgc cttccagctt cgccagttgc agcccggggg tattcaaata
     2521 taagctgaat tcttccgccg acatacatcc gcggagtaac gcataatggt aacgtaacgc
     2581 ctccagcaat tgcttacggc taagattcgc cgcaaggtaa gggcgatgca gacgcacagg
     2641 cagtcgcggt tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2701 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2761 acggcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2821 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     2881 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     2941 agtgtgatta ctaacgtaaa ttttatggtt ttgttgatat ttcgttgaag ttaatgaccc
     3001 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3061 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3121 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3181 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3241 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3301 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3361 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3421 cgtcagcaac gtctggcaca aacgcaggcg gtttcacagc aggatctcga caccgccgcg
     3481 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3541 caggcttctc tcgatacggc taaaaccaat ctcgattaca ctcgcatcgt tgccccgatg
     3601 gccggggaag tcacgcaaat caccactctg caaggccaga cggtgattgc cgcacaacaa
     3661 gcaccgaaca ttctgacgct ggcagatatg agcaccatgc tggtaaaagc gcaggtttct
     3721 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt acttggcgat
     3781 ccactgacgc gctacgaggg gcaaatcaag gatgtactac cgacgccgga aaaggttaac
     3841 gacgctattt tctattacgc ccgttttgaa gtccccaacc ccaatggttt gctgcggctg
     3901 gatatgactg cgcaagtgca tattcagctc accgatgtga aaaatgtgct gacgatccct
     3961 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4021 ggcgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4081 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4141 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtaatgagc
     4201 aggttgaggt gctgaagggc atcagcctcg atatttatgc gggtgagatg gtcgcgattg
     4261 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4321 ccaccagcgg cacctatcgc gtagccggtc aggatgttgc cacgctggac gccgatgcgc
     4381 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgctttcgc
     4441 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgctggtctt gagcggaaac
     4501 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4561 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4621 tgaacggcgg tcaggtaatt cttgccgatg aaccaaccgg cgcactggac agccattctg
     4681 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4741 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4801 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4861 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     4921 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     4981 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5041 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5101 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5161 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5221 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5281 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5341 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5401 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5461 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5521 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5581 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5641 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5701 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggt ggtattggtg
     5761 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5821 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     5881 gcctggtcgg tggcgcgttg ggaataactc tgtcactgtt aattgctttc accttgcagc
     5941 ttttcttacc cggctgggag attggttttt caccgttggc actgctgctg gcgtttctct
     6001 gctcgacggc caccgggatt ttatttggat ggttacccgc acgaaatgcg gcacgactgg
     6061 atccagtaga tgctctggca cgagagtaat ttttgagata aaaatgccag ccgatcgggc
     6121 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6181 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6241 tgaacggatt gtccagcttt tagcgttcta taaccatcca tctgaatggt ggaataatga
     6301 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6361 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6421 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6481 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6541 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6601 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6661 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6721 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6781 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6841 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     6901 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     6961 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7021 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7081 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7141 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7201 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7261 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7321 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7381 gttctggtcg ctatctttag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7441 gaagtcagcc gtctcgatgt ggtgaatttt atctctcatg gcacgcgtaa agacgagccg
     7501 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7561 cgtatggaga atttcacgac gaacctgaat cagcttgcgc gcgtgggcgg aatcgaccca
     7621 ctgattggtc gtgagaagga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7681 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc ggaaggtctt
     7741 gcctggcgaa ttgttcaggg cgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7801 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7861 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     7921 cacaccatta tcggtgcggg ggcagcgtct ggtggtcagg ttgatgcggc taacctaatc
     7981 aaaccgttgc tctccagcgg taaaattcgt gtaattggtt cgacaaccta tcaggagttc
     8041 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8101 gaaccgtcaa tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8161 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8221 tacatcaatg atcgtcatct gccggataaa gcgattgatg ttatcgacga agcgggcgct
     8281 cgcgcacgcc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8341 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8401 accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggtcagga taaagccatt
     8461 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8521 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8581 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8641 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8701 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8761 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8821 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac caccaacgcc
     8881 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     8941 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9001 atctggtttg atcatctgtc aaccgacgtg atccatcagg tggtggataa attcatcgtc
     9061 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9121 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg ctcgtccgat ggcgcgtgtc
     9181 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9241 ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg agctgactta cggattccag
     9301 agtgcacaaa agcacaaggc ggaagcagcg cattaatcta attgttaggt aggttggtga
     9361 agtccgtaat ctcgaaagag gttacggact ttttgtttat ggggtggaac ggtgaaaacc
     9421 ctatttttgg aggtgaaggt aatttgttga taattagtgc tgctggaaaa ggtaaggata
     9481 aaaaagggtg acagcaggaa aatgagatgg ttttgcttta ttaacaacgg actaaacgtg
     9541 tagtatttga gttcactgcc gtacaggcag cttagaaatc gacggggtgc ggtaaaacct
     9601 ttgcgaacgc gttcactgcc gtacaggcag cttagaaatt cacaggtaac atactccacc
     9661 cgcccaccat gttcactgcc gtacagaaag ataaaatgcg aaaaaaaagc tcgcactttc
     9721 gtacgagctc ttctttaaat atggcggtga gggggggatt cgaacccccg atacgttgcc
     9781 gtatacacac tttccaggcg tgctccttca gccactcgga cacctcacca aattgttttg
     9841 ctaccaaacc tcatgggtgg caacggggcg ctactatagg gagttggagt aaaacggtca
     9901 agaagaattt taatgataat tattgtttgc tcatactgta aacaagttgt gcagtatatc
     9961 tacatcgaga caagttacgg acttatactt ccaaagtact tcatacatat cacaaaataa
    10021 aaaggccggt taaaccgacc ttttactcgt tctttctctt cgcccatcag gcggtaaaac
    10081 aatcagcgac tacggaagac aatgcggcct ttgctcaggt cgtacggggt cagttcaaca
    10141 gtcactttgt cgcccgtcag gatgcggatg tagtttttgc gcattttacc ggagatgtgt
    10201 gcagtaacca cgtgaccgtt ttctaactct acgcggaaca tggtattagg caacgtttca
    10261 agaacggtac cttgcatttc aatattgtct tctttggcca tctaatcctc tggggtatca
    10321 ctaccgtaat ttgaaccggc aagataatgc cgaagttctg taaataagta aagatttgcg
    10381 cgctaaatcg caacaaacag gttcggcaca ttactccgaa aacacacggc taagccgcac
    10441 caaaagcgca acgtataagg gagcggtgag ataaacgatg ggcgttacct gacgcgaaaa
    10501 attccttatc ggcagcgggg taatgagcat aaccaactct gcgaccgcaa ttataacact
    10561 ctggggagaa atgtgccgaa aacattcatt tttgtggtga aaacaagcat cgtggcaccc
    10621 agaaattatt cggcaatcgt ccgaggcgca tttgattgag ataattcaga taatcccgac
    10681 ggggaatttc gcaggcacca agcgatgctg tgtgatcgtt aaggacctgg cagtcgataa
    10741 gcttaccgcc atgactgata aattcctcac agaataccag aagcgccgtt ttagacgcat
    10801 tttccatccg gctgaacatg gactcgccac aaaatagcgt tccctgggcc acgccgtaca
    10861 taccgccgac aagctcatct tcatgccaga cttcaatgga gtgggcatga ccgagttcgt
    10921 gaaggcgatg gtaggcttcg accacgccac gcgtgatcca ggttccttct tcgcgatcgc
    10981 tggcacaacc ttcaatgacc tgaccaaaag cgtaattcat cgtgacacga tagggcgagc
    11041 gtttatgaaa tcgcttcata ctacggctga tatgcagtga ttcaggccaa agcaccgcgc
    11101 gaggatcggg cgaccaccag aggatggggg cgcctggaga aaaccacgga aaaataccac
    11161 gctggtaagc cattaacagg cgcgcagggc taagatcgcc ccccagagcc agcaggccgt
    11221 taggctcacg taatgcgcct tccggggaag ggaaggctat tgaatggcga gaaagctgaa
    11281 ccaggcgcat gaccgcaaaa ctccacgcaa gtcggatcgt tcaataatag cttacaaacc
    11341 ctgcttaaac tggtaataac gcccctgtct ggcaagcagt tctgcgtgag taccttgctc
    11401 aataatttgc ccgttgtcca tcactattat ttgttggaaa cgagagagtc cgcgaagtcg
    11461 atgggtgacc attaacaccg ttttctcacg catcatttct gcaagcaatt caaggatctg
    11521 gctttcggtt gtggcatcta agccttcggt aggttcatcc agcaacacca gtggcgcatc
    11581 atgtaacagc gcacgggcga tagccagacg gcgcagttca ccaccggaga gctggcgtcc
    11641 gccttcacct aaccaactgt tgagacctgc gtcctcgagc agcttctcca gaccaacgcg
    11701 acgcaagatc tccgacagag cctcatcact actgccaggc gaggcgagta acagattatc
    11761 acgcagcgtg gcgctaaaca gatgcactcg ctgaggaaca acgctgatgg tctgtcgtag
    11821 agccgcttca ttcaggctgg ctatggggct atcgttaagc aaaatctcgc cctgttgcgg
    11881 gtcccaagcg cgggtcagca gttgtaacag tgttgatttg ccgcatccgg ttcgcccgag
    11941 aatcgcaata tgttccccgg cgtttacctg aagagaaatc cctttaagtg cctgttggga
    12001 ttgctccgga taagtgaact gaacatcccg taatgtcagc gaaacgcgat cggcaacacg
    12061 agtttgggta tcagggaagg tgacttccgg tttttgatcc gttaaatcag tgatccgaac
    12121 ggcagaggca atgacttgcc ccagatgctg aaatgcaccc gttactggtg ccagtgcttc
    12181 aaacgcggct aacgcgcaga agacaaacag ggcaattaac gcgccgggtt gagcattgcc
    12241 gccaacgccg ccagacgcca tccacagcat caggatcacc gctaacgcgc caatgagcag
    12301 cattatcgct tgcgacaatg cggtcagttc agattgacgg cgttgcgctt ccagccattg
    12361 aatttctgta ttctctagtt gcgtgcgata acgatcgctg gcaccaaaaa tggtcagctc
    12421 agcttgccct tgcagccagg ccgtcagttg ttggcgatac tgtccgcgaa gatgagtcag
    12481 attttgcccg gtgctttttc ccgcacgata aaacagcggt ggcatcagga aaagcgtcag
    12541 taacataatg ccgcccagcg taaaggcgag ggtgaaatca aggaaactta acccgactgt
    12601 caccaccata atcaccacaa aagcgcccac cagcggcgag ataacgcgca ggtaaagatg
    12661 atcgagcgta tcaacatccg ccaccacgcg attgagcaat tcgccctgac gatagcgcgc
    12721 cagtccggca ggggagaggg gcaacaattt gctgaaggtg taaatgcgca gatgctgcaa
    12781 cacgcggaaa gtcgcgtcgt gacttaccag acgttcaaaa taacgcccgg cagtacgggt
    12841 gattgcggca ccacgcacgc ccgcagcggg tagcatatag ttgaagctgt acagtccggc
    12901 aacccccgca accgctgagg ccgagaggaa ccagccggaa agtgtcaaca gaccgatact
    12961 ggcgagcagc gtcacaattg ccagcacaat accaagactt aacatccatt tatgacgttt
    13021 atacagtgcc agatagggta gcaaagcgcg catttaaatc tcctcctgac gatgggccag
    13081 taatgtggcg aatgggccac cagccacact taattccgcg taacgtcctt gctcaataat
    13141 ctgaccatcc tgcattaccc aaatgacatc ccagtcagca agatcttcta actggtgggt
    13201 gaccattaac gttgtctggc gcagagaggc ggcattcagc gcctccatta cgcgctgttc
    13261 actgtgagca tcaaggctgg cagcgggttc atccaacagt aatagcgaac agggatttag
    13321 taacgcacgg gccaccgcca cgcgctgcgc ctgccccacg gaaaggcggg cagcctggtc
    13381 gccaacaggc gtatcaacgc cttgtggcag gagcggtaga aactcgctga cccaggcgtt
    13441 atccagcgct gcttgtaatt catgttcgct ggcatcaggt cgcgccagta gtacgttatc
    13501 ccgtaatgtt gctgccggta attgtggatt ttgcccaacc caggagagat gtttacgcca
    13561 tgattctggt gataaatcgc gtaattctat cccgttgatt cgtagcgatc cctgatatga
    13621 gagaaaacca gaaagcgcgt tcaacagtga acttttacct gaaccgctgc gaccaaccaa
    13681 cacggcacgt tggcctgctg gcaaagtaaa gttcagcggt ccggccagcg ttttaccttc
    13741 cggcgacgtg ataaacagat cctcggcctc aatggtcacc ggatcggtcg atgctaattc
    13801 cacctcgcca cgctgcggat gggcgagcgg ggtttccata aacgttttca gactgtcagc
    13861 tgcaccaaca gcctgggctt tagcatgata aaacgtaccg agatcgcgta atggctggaa
    13921 aaactctggc gcaaggatca gggccagaaa acccgcagcc agcgtcacgc cagtatcgta
    13981 gtgaccaaaa tccagctcgc cgagatagga aaaaccaaag tagaccgcca ccagagcaat
    14041 tgacagcgag gtaaaaaatt cgagaatgcc ggaggataaa aatgccagcc gtagcacttc
    14101 cattgtccgt tggcggaaat cttccgaagc agaacgaata ctttcaattt cagcttcacc
    14161 acgaccaaaa atacgcaatg tttccatgcc gcgcaggcga tcgaggaaat gcccacttaa
    14221 gcgagcaaga gcgagaaagt tacgtcggtt agcatcggca gcccccattc caaccagcgc
    14281 cataaacaac ggaattaacg gtgcagtgcc cagcagaatg agcgccgcag cccagtttga
    14341 ggggaagatt gccaccacaa tcagcaacgg caccgacact gccagcgcca tttgcggcag
    14401 gtagcgtgca tagtaatcat gcatatcgtc aatttgctcg agtaccagcg tcgcccagct
    14461 ccccgcaggt ttaccctgaa tccacgctgg ccctgcttgt tgcagacggt cgagaacctg
    14521 acggcggatg gcaaagcgga tatgctgccc ggcgtgataa cccacccgtt cgcgtaacca
    14581 gaccgcccat gcgcgcagta caaaggtcag aaccagtaac gtaaagggaa gcagcagggc
    14641 ttcacgggga atattctcca taatcatatg ttgcagaatt cgcgccatga accaggcctg
    14701 ggcaatgatc aatatgccgc ttacaaagcc cagcagacga gaaatattca gccaacgttg
    14761 ggagatgacg ctttgctgtt ttaaccagcg ggttaactct ttttgacgag atttattcat
    14821 tgcacgctta gcaggtgagt tatcagaatt atttgcagag caatgttaca acggggaaaa
    14881 aataaaggcg acccatagtc gcaaggtgtc gccttcttta cttttgttac tgatttgtaa
    14941 aattattttg cgtcagctaa accatcgagg tagcgttccg catcaagtgc tgccatgcag
    15001 ccagtaccgg ccgaagtaat ggcctggcga taaatgtgat ccatcacgtc gcctgcggca
    15061 aagacgccag gaatgctggt ctgggtggca ttaccatgaa tacccgactg tactttgatg
    15121 tagccgtttt ccagttccag ctgcccttcg aaaatcgcag tattcgggct gtgaccgata
    15181 gcaacaaaca gaccggcaac gtcgagtgac tcgatgttat cgctgttttg cgtatcgcgc
    15241 agacgaacgc cagtgacacc catttgatcg ccggtcactt cttccagcgt acggttggtg
    15301 tgcagaatga tgttgccgtt ctccacttta tccatcagac gcttaataag gattttttcc
    15361 gcgcggaaac cgtcacggcg gtgaatcaga tgcacttccg aagcgatgtt agacagatac
    15421 agcgcctctt caaccgcggt attgccgccg ccgatgaccg caactttctg gttgcgatag
    15481 aagaaaccgt cgcaggttgc acaagcagaa accccacggc ctttaaaggc ttcttcagag
    15541 ggcaggccga gatagcgtgc agaagctccg gtggcaataa tcagcgcgtc gcaagtgtat
    15601 tcgccgttat cgccattcag acggaacgga cggttttgca gatccacctt gttgatatga
    15661 tcaaaaatga tctcagtttc aaacttggtg gcatgttcgt gcatgcgctc cattaataac
    15721 ggaccggtca gatcgtttgg atcaccaggc cagttttcca cttccgtggt ggtggtcagt
    15781 tggccgcctt tttccatgcc ggtaatcagc acaggttgca ggttggcgcg cgccgcgtag
    15841 acagcagcgg tgtatcccgc cgggcctgaa cccaggataa gcagtttact gtgtttggtc
    15901 gtgcccatga gatccccata gttgttggca gacaatgggc aggattgtag ggaatttaca
    15961 gacgtaaaaa aagagtatga cgattttgtt aacaatttgt gcaatcggca gcatcgataa
    16021 gcaggtcaaa ttctcccgtc attatcacct ctgctactta aatttcccgc tttataagcc
    16081 gattaaatga tgaataaacg cccctgttaa tgaatatctg gcatgttgta ctaaaaatcg
    16141 atgttttgct ttgacaatcc cctggtgttt tgcgaaaaca ttcgaggaag aaaaaaaaca
    16201 gtattcttat atgcgcataa ccatgcatgt aaataccatg tttaccgtgc tagtgaaatc
    16261 tacgtatggc gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct
    16321 tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac
    16381 gggtagcgac tctgaacagt gatgtttcag gggcagacag gagtagggaa ggaatacaga
    16441 gagacaataa taatggtaga tagcaagaag cgccttggca aagatctcga ccgtatcgat
    16501 cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa
    16561 cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg
    16621 tttattcagg gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta
    16681 ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct
    16741 gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg
    16801 ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg
    16861 cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt
    16921 aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat
    16981 tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt
    17041 tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg
    17101 aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcgttgc
    17161 tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt
    17221 cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc
    17281 ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc
    17341 ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca
    17401 ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct
    17461 gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg
    17521 gcagcttact aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc
    17581 tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg
    17641 ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc
    17701 gcgatgatac ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa
    17761 atcacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc
    17821 gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt
    17881 tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg
    17941 ccgaccctga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat
    18001 acgatccatt attaaacggt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta
    18061 ccacggcggc acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg
    18121 cctctgttga tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac
    18181 aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag ccacaatatg
    18241 cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagcca cagcagccgt
    18301 attatgcacc tgcagctgaa caacctgtgc aacaacctta ctacgctcct gcggcggaac
    18361 aacctgttca gcaaccgtat tacgcccctg cgccagaaca accggtggca ggtaacgcct
    18421 ggcaagccga agagcagcaa tccacttttg ctccacagtc tacataccag actgagcaaa
    18481 cttatcagca accagccgct caggagccgt tgtaccaaca gccgcaaccc gttgaacagc
    18541 agcctgttgt ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt ccgccacttt
    18601 actattttga agaagtggaa gagaagcgag cccgtgaacg tgaacaactt gcggcctggt
    18661 atcaaccgat tccagaaccg gttaaagaac cagaaccgat caaatcttcg ctgaaagcac
    18721 cttctgttgc agcagtacct ccagtagaaa ccgctgccgc tgtttctccg ctggcatctg
    18781 gcgtgaaaaa agcgacactg gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca
    18841 gtctggcaaa tagcggtgga ccgcgtccgc aggtcaaaga ggggattggc ccgcagttgc
    18901 cacgaccgaa acgtatccgc gtgccaactc gccgtgaatt ggcgtcttac ggtattaagc
    18961 tgccctcaca gcgtgcggcg gaagaaaaag cccgtgaagc ccagcgcaat cagtacgatt
    19021 ctggcgatca gtacaacgat gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc
    19081 agttcgccca gacacagcag caacgctatg gcgaacagta tcaacatgat gtgcccgtaa
    19141 acgcagaaga tgcagatgct gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc
    19201 aacaacaacg ttattccggc gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt
    19261 ttgaattttc gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg ttgtttacgc
    19321 caattgttga acctgtacag cagccgcaac aaccggttgc accgcagcag cagtatcagc
    19381 agccacaaca gccggttgcg ccacagcagc aatatcagca gccgcaacag ccggttgcgc
    19441 cgcagccaca atatcagcag ccgcaacaac cggttgcgcc acagcagcaa tatcagcagc
    19501 cgcaacaacc agttgcgccg cagccgcagt atcaacagcc gcaacaaccg gttgcgccgc
    19561 agcagcagta tcagcagcca caacagccag ttgcgccaca accgcaggat accctgcttc
    19621 atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg acgccgctgc
    19681 cttctctgga tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc
    19741 ttgaacaaat ggctcgcctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg
    19801 ttgtcaatta ctctccgggg ccggttatca ctcgctttga attgaacctg gcaccgggcg
    19861 taaaagcggc gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg
    19921 cggtgcgtgt cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccgaata
    19981 aaaaacgaca aaccgtttat ctgcgcgaag ttttggataa cgccaaattc cgcgataatc
    20041 cgtcgccatt aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc
    20101 tggcgaaaat gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa tctgtcggtg
    20161 tgaacgcgat gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca
    20221 tgatcgaccc gaaaatgctg gagctttcgg tttatgaagg cattccgcat ctgttaacgg
    20281 aagtcgttac tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg
    20341 agcgtcgtta taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa
    20401 aaattgctga agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg
    20461 acagtatgga tgcccagcat ccggtgctga aaaaagaacc atacattgtg gtgttggttg
    20521 acgaatttgc cgacctgatg atgacggtag gtaaaaaagt ggaagagctg atagcacgtc
    20581 tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg
    20641 ttgatgttat tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat
    20701 ccagtaagat tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggga
    20761 tgggggatat gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt
    20821 ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac
    20881 agtatgttga tggcatcact tccgacagtg aaagcgaagg tggcgcgggc ggtttcgatg
    20941 gcgctgaaga actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca
    21001 aagcgtcaat ttctggcgta cagcgtcagt tccgcattgg ttataaccgt gcagcgcgta
    21061 ttatcgaaca gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg
    21121 aagtgctggc tccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg
    21181 tcgcatccgg cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta
    21241 ttagcctgga atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata
    21301 atgatgaaaa aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt
    21361 tgggctgatg ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc
    21421 agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat
    21481 ctgtgggtga aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt
    21541 ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca
    21601 acttggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc
    21661 agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa
    21721 gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat
    21781 cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcaactgaa atcccagcaa
    21841 aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat
    21901 gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac
    21961 ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggccagca
    22021 acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atctgcattc
    22081 tatgatcctc tgggggccac cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg
    22141 ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga aagagattcg
    22201 cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt
    22261 tgacgaagtg caccgattca acaaaagcca gcaggatgca tttctgccac atattgaaga
    22321 cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc
    22381 actgctttcc cgtgcccgtg tctatctgtt gaaatccctg ggtacagagg atattgagca
    22441 agtactaact caggcgatgg aagacaagac ccgtggttat ggtggtcagg atattgttct
    22501 gccagatgaa acacgacgcg ccattgctga actggtgaat ggcgacgcgc gccgggcgtt
    22561 aaatacgctg gaaatgatgg cggatatggc cgaagtcaat gatagcggta agcgggtcct
    22621 gaagcctgaa ttattgaccg aaatcgccgg tgagcgaagc gcccgctttg ataacaaagg
    22681 cgatcgcttt tacgatctca tttccgcact gcataagtcg gtacgtggta gcgcacccga
    22741 tgcggcgctc tactggtatg cgcgaattat taccgctggt ggcgatccgt tatatgtcgc
    22801 gcgtcgttgc ctggcaattg cttccgaaga tgtcggcaat gccgacccac gggcgatgca
    22861 ggtggcaatt gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc
    22921 cattgctcag gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc
    22981 gtttaaagct gcgctggcgg atgctcgcga acgcccggat tatgacgtgc cggttcattt
    23041 gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc
    23101 tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca
    23161 aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc
    23221 ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg
    23281 cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt
    23341 cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt
    23401 cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga
    23461 agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg
    23521 atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga
    23581 agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc
    23641 tgaaattcgc gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg
    23701 taaagacgaa aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt
    23761 tgaagttcgt gaccacgtga cgctgggtga aatgcactct ggcctcgact ttgcagctgc
    23821 agttaagctg actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg
    23881 cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta
    23941 tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc
    24001 tggcgatctg ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct
    24061 gatcccaacg gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga
    24121 tgatctgcca attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata
    24181 tggtcgtgac acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca
    24241 gatcgtgcgc ccggaagact caatggcggc gctggaagag atgactggtc atgcagaaaa
    24301 agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg
    24361 ctttggcgct tgcaaaactt acgacctgga agtatggatc ccggcacaga acacctaccg
    24421 tgagatctct tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg
    24481 ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc
    24541 tgttggtcgt acgctggttg cagtaatgga aaactatcag caggctgatg gtcgtattga
    24601 agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat
    24661 ttttctgaat ctaaaaagcg cctgcgggcg ctttttttgt ctccctttga taccgaacaa
    24721 taattactcc tcacttacac gtaatactac tttcgagtga aaatctacct atctctttga
    24781 ttttcaaatt attcgatgta tacaagccta tatagcgaac tgctatagaa ataattacac
    24841 aatacggttt gttactggaa tcaatcgtga gcaagcttga gtgagccatt atgaaaacga
    24901 aaatccctga tgcggtattg gctgctgagg tgagtcgccg tggtttggta aaaacgacag
    24961 cgatcggcgg cctggcaatg gccagcagcg cattaacatt accttttagt cggattgcgc
    25021 acgctgtcga tagcgccatt ccaacaaaat cagacgaaaa ggttatctgg agcgcctgta
    25081 cagttaactg tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat
    25141 atgtcgaaac ggacaatacc ggcgatgaca attacgacgg cctgcaccag gttcgcgcct
    25201 gcctgcgtgg gcgttccatg cgtcgccgtg tctacaatcc ggaccgcctg aaatatccga
    25261 tgaaacgagt cggggcgcgc ggtgaaggca aattcgagcg cattagctgg gaagaagcct
    25321 acgacatcat cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac gagtctatct
    25381 atctgaacta tggcaccggt acgctgggcg gcaccatgac ccgctcctgg ccgccgggaa
    25441 ataccctggt cgcgcggctg atgaactgct gcggcggcta tctgaaccat tacggcgact
    25501 actcctccgc gcaaattgcg gaaggtttga actataccta cggcggctgg gcagatggca
    25561 acagcccgtc ggatatcgaa aacagtaagc tggtagtgct gtttggtaat aaccctggcg
    25621 aaacgcgaat gagtggcggt ggggtgactt actatcttga acaggcacgc cagaaatcta
    25681 atgcccgcat gatcatcatc gatccgcgct ataccgacac cggtgccggg cgcgaagatg
    25741 agtggatccc tattcgtccg ggaacagatg ccgcactggt taacggtctg gcgtacgtca
    25801 tgatcactga aaacctggtg gatcaggcat tcctcgataa atattgcgtt ggctacgatg
    25861 agaaaaccct gccagccagt gcgccgaaaa atggccacta taaagcttat attctgggtg
    25921 aagggccaga tggcgtggct aaaacaccgg aatgggcctc acaaatcacc ggtgttccgg
    25981 cagacaaaat catcaaactg gctcgtgaaa tcggcagtac caaaccggcg tttatcagcc
    26041 agggatgggg cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct atctcgatgc
    26101 tggcgattct gaccggtaac gttggtatta atggagggaa cagcggcgcg cgtgaaggtt
    26161 catacagctt accgtttgtc cgtatgccga ccttggaaaa cccgatccag accagcattt
    26221 cgatgtttat gtggaccgat gccattgaac gtggcccgga aatgacggcg ctgcgtgatg
    26281 gtgtacgcgg gaaagataag ctggatgtgc cgatcaaaat gatctggaac tatgccggta
    26341 actgcctggt taaccagcat tctgaaatca accgtaccca tgaaatcctt caggatgata
    26401 agaagtgcga gctgattgtg gttatcgact gccacatgac ctcatcggcg aaatatgctg
    26461 acatcctgct gcctgactgc accgcttccg aacagatgga ctttgcgctg gatgcatcct
    26521 gcgggaatat gtcttacgtg attttcaacg atcaggtgat taaaccgcgc tttgaatgta
    26581 agaccatcta tgaaatgacc agcgaactgg caaaacgtct tggcgttgag caacagttta
    26641 ctgaaggccg tacccaggaa gagtggatgc ggcatctgta tgcccagtcg cgggaagcga
    26701 ttcctgaact gccaacgttt gaagagttcc gcaagcaggg gatctttaaa aagcgcgacc
    26761 cacaagggca tcacgttgct tataaagcct tccgtgaaga tccgcaggca aacccactga
    26821 ctacgccatc gggcaaaatt gagatttatt cgcaggcgct ggctgacatt gccgctacct
    26881 gggaattgcc agaaggcgat gtgatcgatc cactgccgat ctacacgccg ggctttgaaa
    26941 gttatcagga tccgctgaac aaacagtatc cgctgcagct tacgggtttc cactataaat
    27001 ctcgcgttca ctcaacttac ggcaacgttg atgtgctgaa agcagcttgc cgtcaggaaa
    27061 tgtggatcaa cccgcttgat gcgcaaaaac gcggtatcaa caacggcgat aaagtcagga
    27121 tctttaacga tcgtggtgaa gttcatattg aggcgaaagt gacgccacga atgatgccag
    27181 gtgtggtcgc actgggtgaa ggtgcctggt atgacccgga tgcaaaacgt gtcgataagg
    27241 gtggttgtat taacgtactg accactcaac gtccgtctcc tctcgctaag gggaatccgt
    27301 cacatacaaa ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac aacccagtat
    27361 ggatttttta ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct ggcttgtaaa
    27421 gactacaaag atttgacgcc agaagtcagc ttccgccgca tttatgaata tgctggcggc
    27481 gactggcagg aagataacgg tgtctggcac cagaacgtgt ttgcctacta tctgtcgatt
    27541 tcgtgcaacc actgcgaaga tccggcttgt actaaagtct gcccgagcgg tgcgatgcat
    27601 aaacgtgaag atggttttgt tgtggtcgat gaagatgtgt gcattggctg ccgctactgc
    27661 catatggctt gcccgtatgg cgcaccgcaa tataacgaaa cgaaaggcca tatgaccaaa
    27721 tgcgatggtt gttatgaccg tgttgcggag ggtaaaaagc cgatctgtgt tgaatcttgt
    27781 ccgctgcggg cgctggattt cggccctatc gacgagctgc gtaaaaaaca tggcgatctg
    27841 gcggccgttg cgccgttgcc gcgagctcac tttaccaaac cgaatattgt gatcaaaccc
    27901 aatgccaata gccgcccgac cggggatacc actggctatc tggcaaaccc gaaggaggtg
    27961 tgagatggga agtggatggc atgaatggcc gctgatgatc ttcacggtct tcgggcaatg
    28021 tgtggcgggt ggttttatcg ttctggcttt ggcgctgctc aaaggcgacc tgcgagcaga
    28081 agcccagcag cgtgttatcg cctgcatgtt tggcttatgg gtgctgatgg gcattggctt
    28141 tatcgcttct atgctccatc ttggttcacc gatgcgcgct tttaactcgc tcaaccgggt
    28201 aggggcttca gcactcagta acgaaatcgc cagcggttcg atcttttttg ccgtaggcgg
    28261 catcggctgg ttgctggcaa tgctgaaaaa gctgtcaccg gcattgcgta cgctttggct
    28321 ggtggtgacg atggttcttg gcgtcgtctt tgtctggatg atggtgcgtg tgtataacag
    28381 cattgatacc gttccgacct ggtacagcat ctggacgccg atgggcttct tcctgacgat
    28441 gttcatgggc ggcccgttac tggggtacct gttgctgagt ctggctggcg tcgatagctg
    28501 ggcgatgcgt ctgctgccag ccatttccgt actggcactg gtggtgagtg gcgtggtgtc
    28561 agtgatgcag ggcgcagagc tggcgactat tcatagttct gtgcagcagg ccgcagcgct
    28621 ggtgccggac tatggtgcgc tgatgtcctg gcggatcgtg cttttggccg ttgccctgtg
    28681 cttgtggatt gcaccacagc taaaaggtta tcagcctgcg gttccgctac tatccgtctc
    28741 attcattctg cttctggcag gggaattaat cggtcgcggc gtattctatg gtttgcatat
    28801 gaccgtgggg atggccgtcg caagctaatc agtaacaacc ggggtttcgg ccccggttct
    28861 cttttatttc tgcttcgtta acgtgtcata actggtcatc aaattacgat aatcaggaat
    28921 atggttagag aacaacgtcg ccagtccttc aatatcatta cgccagtcac ggtgcagctc
    28981 gcaagccacg ccgaaccatg tcatcaattg cgcacctgct tgcgacatgc gatcccatgc
    29041 cgaatgccgg gtaatttcat taaaggtccc tgacgcgtca gtcacaacaa acacatcaaa
    29101 cccttcttca atggctgaaa gcgccgggaa tgccacgcaa acttcggtta ccacaccggc
    29161 aataattaac tgttttttac ctgtcgcttt gactgctttt acaaaatctt cgttatccca
    29221 ggcgttaata ttgcccgggc gagcaatgta aggtgcatcg gggaattgtg ctttcagttc
    29281 tggaactaat gggccgttag gaccggtttc aaaactggtg gtaagaatag ttggtaagtt
    29341 gaaatactta gccaggtcac ccagcgccag cacgttattt ttaaacttat cgggttcgat
    29401 atcccgtaca agggaaagta aaccagcctg gtgatcaaca agcaaaacag cagcatcatt
    29461 tttatcaaga cgaacatacg gtttggtcat cctcttctcc tttcgagagt cagtggtacg
    29521 cctgaaatcg gtcagacgtg atgcattgct ctgaaagcat agacgggaaa tatgagtttg
    29581 ctgtgaccat gaaatttttc gactgaacga agcgtatgaa atttgtgtta gatcaataaa
    29641 aacaatcaga tgagattaat catctcttca gtattagcgt tatgaatgag taatataaat
    29701 cgataatacc gccgttgcga tatgcgcgta ttttcaccca ttgacaatgt ttttggcggt
    29761 ggcatgatgc gcatgaaatt tgaacttcct cacggtttta attcatgtcc acgtataccc
    29821 ggcctgtcat gcttttgctg tctggcctgc ttttgttgac tctggcgatc gcggtgttaa
    29881 atacactcgt gccgctttgg ctcgcccagg aacacatgtc cacatggcag gtaggcgttg
    29941 tcagctcatc ctattttacc ggcaaccttg tcggtacatt gctgacaggg tatgtcatta
    30001 aacgcattgg ttttaaccgc agctattatc tggcctcctt catttttgcc gctggctgtg
    30061 ccggccttgg cctgatgatt ggattctgga gttggttggc gtggcgtttt gtcgcgggcg
    30121 tcggctgtgc catgatttgg gtagtcgttg agagcgcgct gatgtgcagt gggacgtcac
    30181 gtaaccgtgg gcgtttgctt gctgcataca tgatggttta ttacgtggga acgtttttag
    30241 gccagttact ggtcagcaag gtctcaaccg agctgatgag cgtattgccg tgggttacag
    30301 gtttgacatt ggcagggatc ttaccgctgt tgtttacgcg tgtcctgaat cagcaggctg
    30361 aaaaccatga ttcgacgtca attacggcaa tgctaaaact ccgtcaggcg cggcttggcg
    30421 tgaatggctg cattatctca ggaatcgttc tgggatctct atatggcctg atgcctctgt
    30481 acctcaatca caaaggggtg agcaatgcca gtattggttt ctggatggcg gtactggtca
    30541 gtgcgggtat cctcggacaa tggccgattg gacgtctggc ggataagttt ggtcgactgc
    30601 tggttttgcg tgttcaggtc tttgtcgtca ttctcggcag tattgcgatg cttagccagg
    30661 cggcgatggc cccagcgtta ttcatcctcg gtgccgctgg ctttacgcta tatccggtgg
    30721 cgatggcatg ggcttgcgag aaagttgaac atcatcaact ggtggcgatg aaccaagcct
    30781 tactgttgag ctatactgtg ggaagtctgc ttggcccgtc atttaccgct atgctaatgc
    30841 agaatttctc cgataattta ttgtttatca tgatcgccag cgtatcgttt atctatttgc
    30901 tgatgctgct gcgcaacgcc ggtcatacgc cgaaacccgt tgctcacgtg taaatgaatt
    30961 caagcagagt gtgaacttac tgtttcacac tctgcttttt tgtttcttct atctgacttg
    31021 ctttattcca aattttattc gtttaaaaat aaaatgtgta gcaggttata attttgcatt
    31081 tcgctatttc cgcacttctt atttgccgcg cataatccct cgttttacag atgcccctat
    31141 aattttggcg aaggatttgt ctatggctgg gaatgttcag gaaaaacagc tgcgatggta
    31201 caacattgcg ctgatgtctt ttatcactgt ctggggtttt ggcaacgttg ttaataacta
    31261 cgccaaccag gggctggtgg ttgttttttc atgggtgttt atctttgcgc tctatttcac
    31321 accttatgcg ctaattgttg gtcagttagg ctcgaccttc aaagatggga agggcggggt
    31381 cagtacctgg attaaacaca cgatgggacc cggactggct tatctcgccg cgtggaccta
    31441 ctgggtggtg catattccct atctggcaca aaaaccccag gcaattctga ttgcgctcgg
    31501 ttgggcgatg aaaggcgacg gttcgctaat caaagaatat tcagtcgtag cgttacaggg
    31561 gttaacgctg gtgctgttta tcttctttat gtgggttgct tcacgcggta tgaaatcgct
    31621 gaaaatcgtc ggttctgtgg cagggattgc tatgtttgtt atgtcgctcc tgtatgtggc
    31681 gatggcggta accgcgcctg caattactga agtgcatatt gcgaccacaa acattacctg
    31741 ggaaacgttc attcctcata tcgactttac ctacattacc actatttcaa tgctggtttt
    31801 cgcggttggc ggagcagaga agatttctcc ttacgttaat caaacgcgca acccaggaaa
    31861 agaatttcca aaagggatgt tatgcctggc ggtgatggtt gcggtttgtg ccattctggg
    31921 ctcgctggcg atggggatga tgtttgattc gcgtaatatc ccggatgact taatgaccaa
    31981 cggtcagtat tacgcctttc agaagctggg cgagtattac aacatgggta atactttaat
    32041 ggtgatttac gccattgcga ataccctggg acaagtagcg gcgctggtat tctcgattga
    32101 tgccccgctt aaagtgctat taggtgatgc tgacagcaaa tatattccag ccagtttatg
    32161 tcgtaccaac gcttctggta cgcccgttaa tggctatttt ctgaccctgg tactggtggc
    32221 gattctgatt atgctgccga ctctcggcat tggtgatatg aacaatctct ataaatggct
    32281 gttgaacctt aattcggtag tgatgccgct gcgttattta tgggtatttg ttgcatttat
    32341 tgcagtcgtt cgcctggcgc agaaatataa accagaatat gtctttattc gtaataagcc
    32401 tctggcaatg accgtcggga tttggtgttt tgcctttacc gcctttgcct gtttgacggg
    32461 gatcttcccg aaaatggaag ccttcactgc agagtggacc ttccagttgg cgctgaatgt
    32521 tgcaacgccg tttgtgctgg taggattagg actgatattc ccgctgctgg cgcgtaaagc
    32581 gaatagtaaa taattattgt ggtcggtgac gctgactctg gcagatctga cccgttttta
    32641 cataatcgag aaatgcgcgc aagccgcagg acatatactg gcggtttggg taatagatct
    32701 ggaagcctgg acgctctgtg atccagtctt ccagtacaca ttctaaacga ccggtatcaa
    32761 ggtactcctt gatctgttca tagagcagat acccgatacc agcacccatc aaaacggcat
    32821 ccagttcagc atcaacatca
//
LOCUS       DAQSWQ010000042        32650 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.042, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000042 DAQSWQ010000000
VERSION     DAQSWQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.042"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            79..1581
                     /locus_tag="R8G18_002023"
     CDS             79..1581
                     /locus_tag="R8G18_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEE9795722.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKSFSQQEYFPVSADDISLYKEKHYPLWKKQCE
                     EILKNCHTHINPKAINYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGFNWEIMEELT
                     ESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGPLLSLTPTER
                     DKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVYTANKPKKVNGAIEVRVDAN
                     NLTNPVIKTFKLKITIEEHSAFSTITEMVQTYIQEQKKRQKSSISAQIKT"
     gene            1825..2085
                     /locus_tag="R8G18_002024"
     CDS             1825..2085
                     /locus_tag="R8G18_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HEE9795723.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            2276..2416
                     /locus_tag="R8G18_002025"
     CDS             2276..2416
                     /locus_tag="R8G18_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HEE9795724.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(2723..3022)
                     /gene="ihfA"
                     /locus_tag="R8G18_002026"
     CDS             complement(2723..3022)
                     /gene="ihfA"
                     /locus_tag="R8G18_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEE9795725.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3027..5414)
                     /gene="pheT"
                     /locus_tag="R8G18_002027"
     CDS             complement(3027..5414)
                     /gene="pheT"
                     /locus_tag="R8G18_002027"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEE9795726.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(5429..6412)
                     /gene="pheS"
                     /locus_tag="R8G18_002028"
     CDS             complement(5429..6412)
                     /gene="pheS"
                     /locus_tag="R8G18_002028"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEE9795727.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(6621..6745)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(6695..6739)
                     /gene="pheM"
                     /locus_tag="R8G18_002029"
     CDS             complement(6695..6739)
                     /gene="pheM"
                     /locus_tag="R8G18_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HEE9795728.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(6862..7218)
                     /gene="rplT"
                     /locus_tag="R8G18_002030"
     CDS             complement(6862..7218)
                     /gene="rplT"
                     /locus_tag="R8G18_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEE9795729.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(7271..7468)
                     /gene="rpmI"
                     /locus_tag="R8G18_002031"
     CDS             complement(7271..7468)
                     /gene="rpmI"
                     /locus_tag="R8G18_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEE9795730.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(7565..8107)
                     /gene="infC"
                     /locus_tag="R8G18_002032"
     CDS             complement(7565..8107)
                     /gene="infC"
                     /locus_tag="R8G18_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEE9795731.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(8111..10039)
                     /gene="thrS"
                     /locus_tag="R8G18_002033"
     CDS             complement(8111..10039)
                     /gene="thrS"
                     /locus_tag="R8G18_002033"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEE9795732.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            10564..12462
                     /gene="espL1"
                     /locus_tag="R8G18_002034"
                     /pseudo
     CDS             10564..12462
                     /gene="espL1"
                     /locus_tag="R8G18_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            12634..12741
                     /locus_tag="R8G18_002035"
     CDS             12634..12741
                     /locus_tag="R8G18_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795733.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(12794..13552)
                     /locus_tag="R8G18_002036"
     CDS             complement(12794..13552)
                     /locus_tag="R8G18_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HEE9795734.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            13839..14768
                     /gene="pfkB"
                     /locus_tag="R8G18_002037"
     CDS             13839..14768
                     /gene="pfkB"
                     /locus_tag="R8G18_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HEE9795735.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            14869..15159
                     /gene="ghoS"
                     /locus_tag="R8G18_002038"
     CDS             14869..15159
                     /gene="ghoS"
                     /locus_tag="R8G18_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HEE9795736.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            15265..16125
                     /locus_tag="R8G18_002039"
     CDS             15265..16125
                     /locus_tag="R8G18_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEE9795737.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(16166..16702)
                     /locus_tag="R8G18_002040"
     CDS             complement(16166..16702)
                     /locus_tag="R8G18_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HEE9795738.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            16849..17517
                     /gene="hxpB"
                     /locus_tag="R8G18_002041"
     CDS             16849..17517
                     /gene="hxpB"
                     /locus_tag="R8G18_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HEE9795739.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            17680..18270
                     /locus_tag="R8G18_002042"
     CDS             17680..18270
                     /locus_tag="R8G18_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEE9795740.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            18403..19794
                     /gene="tcyP"
                     /locus_tag="R8G18_002043"
     CDS             18403..19794
                     /gene="tcyP"
                     /locus_tag="R8G18_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HEE9795741.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(19798..20614)
                     /locus_tag="R8G18_002044"
                     /pseudo
     CDS             complement(19798..20614)
                     /locus_tag="R8G18_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20891..21154)
                     /gene="cedA"
                     /locus_tag="R8G18_002045"
     CDS             complement(20891..21154)
                     /gene="cedA"
                     /locus_tag="R8G18_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HEE9795742.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            21337..23598
                     /gene="katE"
                     /locus_tag="R8G18_002046"
     CDS             21337..23598
                     /gene="katE"
                     /locus_tag="R8G18_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HEE9795743.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(23856..24614)
                     /gene="chbG"
                     /locus_tag="R8G18_002047"
     CDS             complement(23856..24614)
                     /gene="chbG"
                     /locus_tag="R8G18_002047"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HEE9795744.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(24627..25979)
                     /gene="celF"
                     /locus_tag="R8G18_002048"
                     /gene_synonym="chbF"
     CDS             complement(24627..25979)
                     /gene="celF"
                     /locus_tag="R8G18_002048"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEE9795745.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(26084..26926)
                     /gene="chbR"
                     /locus_tag="R8G18_002049"
     CDS             complement(26084..26926)
                     /gene="chbR"
                     /locus_tag="R8G18_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HEE9795746.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(26934..27284)
                     /gene="chbA"
                     /locus_tag="R8G18_002050"
     CDS             complement(26934..27284)
                     /gene="chbA"
                     /locus_tag="R8G18_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HEE9795747.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(27335..28693)
                     /gene="chbC"
                     /locus_tag="R8G18_002051"
     CDS             complement(27335..28693)
                     /gene="chbC"
                     /locus_tag="R8G18_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HEE9795748.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(28778..29098)
                     /gene="chbB"
                     /locus_tag="R8G18_002052"
     CDS             complement(28778..29098)
                     /gene="chbB"
                     /locus_tag="R8G18_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HEE9795749.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(29397..29735)
                     /gene="osmE"
                     /locus_tag="R8G18_002053"
     CDS             complement(29397..29735)
                     /gene="osmE"
                     /locus_tag="R8G18_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HEE9795750.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            29937..30764
                     /gene="nadE"
                     /locus_tag="R8G18_002054"
     CDS             29937..30764
                     /gene="nadE"
                     /locus_tag="R8G18_002054"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEE9795751.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            30994..31881
                     /gene="cho"
                     /locus_tag="R8G18_002055"
     CDS             30994..31881
                     /gene="cho"
                     /locus_tag="R8G18_002055"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HEE9795752.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(31841..32416)
                     /gene="ves"
                     /locus_tag="R8G18_002056"
     CDS             complement(31841..32416)
                     /gene="ves"
                     /locus_tag="R8G18_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HEE9795753.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         8658 a         8095 c         7434 g         8463 t
ORIGIN      
        1 ggtaagaatc atgctttgat aaccaattga taactattat tatattaatc taacaccatt
       61 gacatagttg aggtttgaat gaacaaaaag ctgtatttat tcccagactc aaacattttt
      121 ctacaatgca aggacttgac gcaggtaaat ttttccgaga ttactgcttg tgatgaagta
      181 tgtatcatta ttacaaggcc cattcagcaa gagattgacc gccagaaagg gcaaggtaat
      241 tcccgcctat ccaaaaaagc aaggaaggct gctagcctat ttaatcaagt agttgattca
      301 actgacatga ctcttgttat tcgtgaacgc tcgccgcgag tttttcttac catggatcta
      361 agcctgaaac catgtgaaca gttatctgaa caattggatt atcaggaggc cgacgatcgt
      421 tttgttggta ttgcggcagg gtattgcgct gacgatccag attcggaggt ggcaatcatt
      481 accaatgata gcggtccacg tttttcagca ataaaacata atataatctg ctataaagtg
      541 ccttcttcat ggttgcttcc tgctgagcca gatgaaaagg ataaacaaat taaattgctg
      601 gaagctaagc tgaagcaatt cactcagtct atgcccgatt tcagcattaa agtagccgat
      661 gacgagcaat taaatattgt acttccttat tatacagctt tatctgaata tgaagttgat
      721 aatttgcttt gtcaactaac ggatgcattc ccattggtta caaactttga caatgaacaa
      781 actaccccac aatcactaga aaaaatcatc aagtcattta gtcaacagga gtatttccct
      841 gtatcagcag atgatatttc tctttataaa gaaaaacatt atccactttg gaaaaagcag
      901 tgtgaagaaa tactgaaaaa ctgtcatact catattaatc cgaaggcaat aaattacccg
      961 ctgtcgtttt tattacaaaa cacaggttat actatcgctg aacgtgctgt ggtaacattt
     1021 acagcaaaag gagattttcg tctgtgtgga tttaattggg agataatgga agagctgact
     1081 gaaagcaaaa ggattgacct accttcagcc cctaccgccc ccaaaggaaa atggcgtagt
     1141 gtatgtggcg taacagaaag aatgccttac gctgggggag ctttggaaac accgctatcc
     1201 agtataattt caaaagaaat tggtcctttg ttatctctta ctccaacaga aagggataaa
     1261 aacgattttt attataaaga aaagagaaat gagccagttc aagttgtatc gctggaatgc
     1321 gaagagtggc gtcatcaagt caaagaagaa atattcccgt tgttcgtata tacagccaac
     1381 aaaccaaaga aagtgaatgg tgcaattgag gtgagagttg atgcaaataa ccttaccaat
     1441 ccggtcatta aaacatttaa attaaaaata actatagaag aacatagtgc attttcaacc
     1501 ataacagaaa tggtgcagac atacattcaa gagcagaaga aaagacagaa aagttctatt
     1561 agcgcacaaa tcaaaacatg agggtgtact caaataatac aatgctatag taaatctata
     1621 agtgcataac tatttcctga tcttttccca aaatagatga caggataaaa aacattccaa
     1681 tcttttacag gctttggata aatcgtatca aagcctattt cttcaagagt taaatcaccg
     1741 cttttcgctc gaaacggact ggctgttgga gtatgagcca gaacaactta gaatagcccc
     1801 tgcactacgt taagggaggt cgctatgttc cgttgtccgc tttgtggcgc atctgcccgt
     1861 atccgcacca gtcgtccgga aaatgattca aacaccgtgc ggcaaaagta ttaccagtgt
     1921 aacaatctgg aatgcggcgt atgcttctca acactggaag ctttccataa attcacatca
     1981 aaacacgcct ccggcgttca ctcttcagaa gatatcccgt ggcatgatct gccagcttca
     2041 cacaggggaa acaatcagat gagcttgcct ttacctcaga attaacaggc agaattgccg
     2101 gagtaacaaa aaagcgatag attacgcgcg ggtgcctttc ggctgatggt cggagggaat
     2161 acccgaaggc cagatgtgga aaggccccgg aaaacatctc tgtttaaccg aggccctaac
     2221 cgcattacct tgacaagtga aaggttagcg cctctccgga aaaggagcaa gtgctatgtc
     2281 gcaaaaatcg cttacgacca tcacgttctg cgtgacggca atcctcatca tctggatgtt
     2341 gcacggttcg ctgtgtgaaa tacggatgag cttctgggga gcggagtttg cggcgttctt
     2401 acagtgtaag cagtaaggaa accgcgacgg gggagcaatc ccccgtcaat cggttgccag
     2461 ggtaaggccg gataaggcac cctatctcac agatatgaac aacaaacccg cagcgtaaaa
     2521 actgcgggtt ttctttttgg tgccctcacc gacttgaaac agtgataaga ctataagaca
     2581 aagcccgcaa tgtagcgggc ttactcccct caccctccac tgaatgtgga cacacagtgg
     2641 aacgaagcgg cataagatat tataaatcaa tcagttattg aaagaaaaaa ggccgcagag
     2701 cggccttttt agttagatca gattactcgt ctttgggcga agcgttttcg acccggcttt
     2761 ttaacttctg cccgggtctg aaggtcacca cgcgccgtgc tgtaatggga atatcctcgc
     2821 ccgttttcgg gttacgtccc gggcgttgat tcttatcacg cagatcgaag ttaccaaaac
     2881 cagagagttt cacctgttcg ccgttttcca gagcgcgacg gatctcttcg aaaaacagtt
     2941 caaccagttc tttggcatcc cgcttgctaa gcccaagctt atcaaacaga tattctgaca
     3001 tttcagcttt tgtaagcgcc ataggttcaa tccctcaatg atgcctggaa tcgctctttt
     3061 aatgcctcta cacatttggc gacggtagcg gcaatctcct cttcttcgag tgtacggctg
     3121 gtatcttgca ggatcaggct tatggcgagg ctcttatacc cctccgcaac acccttaccg
     3181 cggtacacgt caaataagtt tacgccaact acctgattta cgccaacttt cttacattcg
     3241 gataaaatat ccgctgcggg aacgttttct gcgaccacca ccgcgatgtc acgacggttc
     3301 gccgggaagc gagaaatctc gcgcgcctga ggcaccacgc ggtctgcgag cttgttccac
     3361 tccagttcga acaccagagt gcgaccgtta agatccagtt tacgttccag ttcaggatga
     3421 acaactccaa caaaaccaat acgttcccct ttcagataaa tcgctgcgga ttgccccgga
     3481 tgcagtgccg gattcgcttc tgcacggaac tcaacctcat tcagtttacc ggtcaggtcg
     3541 agaacggatt caagatcgcc tttcaaatca tagaaatcaa cggtctcttt tgccaggttc
     3601 cagtgctctt cgtaacggtt accgcaaatc acaccggcta acatcagatc ctgacgaatg
     3661 cccaacggtg cctgagtatc tggtacgaaa cgcagaccgc tttcgaaaat gcgcacacgg
     3721 ttctgctgac ggttctggtt gtacaccacg gttgccagca ggccggtcca cagagaaaga
     3781 cgcattgctg acatttcaac agagatcggg cttggcagca gtaaggcttc aacgcctgga
     3841 tggatcatct gctgcacttt cggatcaacg aagctgtagg tgatcacttc ctgatagcct
     3901 ttgtcgttga gcagcgtttt cacgcgcttg agcgacaggt cagcttcacg gtgagtaccc
     3961 ataatcaggc ttgcctgtac cggctcatcc gggatgttgt tgtagccgta aacacgcgcg
     4021 acttcttcaa ccagatcttc ttcaatctcc atatcgaaac gccagctcgg cgcaactgcc
     4081 tgccactcgt ctttgccttc ggtcacttcg cagccgagac gacgcagaat gtcagttacc
     4141 tgttcatcag caatatgatg gccgatcagg cgatccagtt tgctacgacg taaagtgatg
     4201 gttgcacgct tcggcagcgt tgcttcgttg gtgatatcaa ttaccggacc agcctcacca
     4261 ccgcagatgt cgatcagcag acgggtcgca cgttccatcg ctttgtgctg cagtgccgga
     4321 tcaacgccac gctcataacg gtgagacgca tcagtatgca ggccatgacg acgagcacga
     4381 ccggtgatag acagcgggct gaagaaagcg cattccagca agacgttttg cgtttcgtca
     4441 ttcacgccag agtgttcgcc accaaagatg ccgcccatcg ccagcgcctt gttgtggtcg
     4501 gcgatgacca gagtgtcagc attcagcttc gcttcagtgc catcaagcag caccagcgtt
     4561 tcgccctctt ttgccatccg caccacaatg ccgccttcaa tgcgatcttt atcgaaagcg
     4621 tgcatcggct ggcccagttc gagcagtaca tagttggtga cgtcaacaac tgcatcgata
     4681 gaacggatcc cgcaacgacg cagtttttct ttcatccaca gcggagttgg cgctttaacg
     4741 ttaatgcctt ttaccacacg gccaagatag cgcgggcagg cttcaggcgc ttcgactgta
     4801 atcggcaacg tgtcgtcgat ggtcgcacca accggaacga tttccggttc aaccagcggc
     4861 agctgattca gcacggcaac gtcacgcgca acaccaatga tacctaagca gtcggcacgg
     4921 tttggcgtta cgctgatttc gatggtgttg tcatcgagtt tcaggtattc gcggatgtca
     4981 gtgccaatcg gcgcatctgc aggcagttcg ataatgccgc tgtgatcgtc agaaattccc
     5041 agttcagaga aggagcacag catcccttca gacggttcgc cacgcagttt cgccgcttta
     5101 attttgaaat cacccggcag aacagcacca atggtagcta ccgccacacg caggccctga
     5161 cggcagtttg gcgcaccaca gacgatgtcc agcaggcgat cgccgccgac attcactttt
     5221 gtcacacgca gtttgtcagc gttcggatgc tgcgcacact caaccacttc accaacgacc
     5281 acgccgtgga agctaccggc aaccggttct acaccgtcaa cttccaggcc cgccatagtg
     5341 atttgattcg ccagcgcatc gctatcaatc gccgggttca cccattcgcg taaccacagt
     5401 tcactgaatt tcataatcta ttcctgcctt atttaaactg tttgaggaaa cgcagatcgt
     5461 tttcgaagaa tgaacgcagg tcggtgacgc cgtaacgcaa catagtcaga cgctccatcc
     5521 ccatcccgaa ggcgaaacca gagtaaactt ccgggtcgat gccaacgtta cgcagcacgt
     5581 tcggatgcac catcccgcaa cccagcactt ccagccattt accgttttta cccatgacgt
     5641 caacttctgc agaaggctcg gtgaacggga agtaggaagg acggaagcga atctgcaaat
     5701 cttcctcaaa gaagttacgc aggaaatcgt gcagagtgcc tttcaggttg gtaaagctga
     5761 tgttggtatc aacaatcaga ccttccatct gatggaacat cggcgtgtga gtctggtcgt
     5821 agtcgttacg ataaacgcgg ccaggcgcga tgatacgaat cggtggctgc tgggctttca
     5881 tggtgcggat ctgtacgcca gaggtctggg tacgcagcag gcgggtagtg tcaaaccaga
     5941 aagtgtcgtg gtcagcgcgc gccgggtggt gaccaggaat gttcagagca tcgaagttat
     6001 gataatcgtc ttcgatttcc ggcccggttg ccacggtaaa gccaagctca ccgaagaaac
     6061 tttcgatacg gtcgatggta cgggtaaccg gatgcagacc gccgttttca atgcgacgac
     6121 ctagcaggga gacatcaatc gtttccgccg ccagacgcgc attcagcgct gcgctttcca
     6181 gttccgcttt acgcgcattc agcgcctgct gaacctgctc tttcgcttcg ttgataaccg
     6241 caccagctgc cggacgctct tctggcggca gctcacgcag ggtcgtcatc tgaagggtta
     6301 agtgcccttt tttacccaaa tattcgacgc gcacattatc taacgcggca acatctgacg
     6361 cctggctaat ggccgccttc gcactggcaa ccagttctgc gagatgtgac atggttttcc
     6421 tcattgtgtc agtggtgaca ctggttcgtt ggacttagag cctatcccat caggctattt
     6481 tacttgccat tttggtcccg ggcagtgctc gaaattctca cgtacttaaa tacgctccgg
     6541 tttctccgcg ctggccgtgt ccagactggc tgcgacaatt acgcctgata gactggcttt
     6601 ttatttttca aaacgcgcat acaaaaaaag cctccactgg gaggctttca ggcgctgttt
     6661 tccgtttctc ttctcacgcg ctagcctcct ggattcaggt gctaaagtaa aaaaagaagc
     6721 ggaaaatagc agcattcatt gcttgcgtta ccttttggta ctcttcaaaa gacctttatt
     6781 gaaaaggcta cggcgataaa agtcaatgtt ttgatggcgt tgaaacgaaa agagggagac
     6841 tagctccctc tttcaactgg cttatgccag agctgctttc gctttttcaa ccagagcggt
     6901 gaacgctact ttgtcgaata ctgcgatatc agccaggatc ttacggtcga tttcaacaga
     6961 ggcttttttc aggccattga tgaatttgct gtaagaaata ccgttctgac gtgctgctgc
     7021 gttgatacgc gcaatccaca gttgacggaa ctgacgctta cgttgacgac ggtcacggta
     7081 agcatactga ccagctttga taacagcctg gaaggcaacg cggtatacgc gagaacgcgc
     7141 accgtagtag cctttagctt gtttcaaaat tttcttgtga cgtgcgcgtg caataacacc
     7201 acgttttacg cgagccatat gtgctctcct gtatctatat tctaattaaa aagttaaaaa
     7261 cgttaacggc ttatgcgtac ggcaggcacg cgattaccag gcccagatcg cctttggaaa
     7321 ccatggcttt cggacgcagg tgacgtttac gtttggtcgc ttttttggtc agaatgtgac
     7381 gcaggttagc gtgcttgtgc ttaaaaccac ctttaccggt ttttttgaag cgcttagcag
     7441 caccgcgtac ggtcttaatt tttggcattt taataacttc cacttcgcat tgttaataaa
     7501 cgaaacaaag gcgaacaaag cctgtgaagc ccgaaggctc cacagacagt gctacttgaa
     7561 ggccttactg tttcttctta ggagcgagca ccatgatcat ctggcggcct tcgatcttcg
     7621 ttgggaagga ttcgaccact gccagttctt gcaaatcgtc tttcacgcga ttaagcactt
     7681 ccataccgat ttgctggtgc gccatctcac gaccgcggaa acgcagcgtg attttggctt
     7741 tatcaccctc ttcgagaaag cgaatcaggc tgcggagttt tacctgatag tcgccttcat
     7801 ctgtaccagg acggaattta atttccttaa cctggataac tttttgcttt ttcttctgtt
     7861 ccttagaaga cttgctcttt tcatagagga atttgccgta atccattata cgacaaaccg
     7921 gcggctcggc gttagggctg atctcgacta agtctactcc ggcttcttct gctttctcca
     7981 gagcttctct cagactcaca ataccaagct gctcgccttc cagacctgtt aagcgaactt
     8041 cctgggcgcg aatttcgcca ttgatacggt tagggcgcgc cgtttgaact cgttttccgc
     8101 ctttaatacc ttattcctcc aattgtttaa gactgcggct gcgaatctct tgttgcagct
     8161 tctcgatcac ttcatttacg tccatgcttc ccaggtcttt accacggcgg gtgcgaacgg
     8221 caactttgcc tgattccacc tctttatcac cacagaccag catatatggg acgcgacgca
     8281 aagtgtgctc gcggatttta aagccaatct tctcatttct caagtctgct ttaacacgaa
     8341 tgcccgcatt tgatagtttt tgcgtcaatt cgttaacgta ttcagactgt gaatcggtaa
     8401 tattcatgat aacaacctga actggcgcaa gccaggtcgg gaagaaacca gcgaattctt
     8461 cggtcaggat accgatgaaa cgttccatcg accccagaat tgcgcggtga atcattaccg
     8521 gtaccttacg ctcgttgtct tcgcccacat aggaggcgct cagacgagac ggcaaggaga
     8581 agtccagctg tactgtaccg cactgccatg cacgatcgag gcagtcatac agggtaaatt
     8641 caattttcgg accgtagaaa gcgccttcac ccagttgata ttcaaacggg atgttgtttt
     8701 cttccagcgc aaccgccagg tccgcctcag cacgatccca catttcgtcg ctgccaatac
     8761 gtttttcagg acgagtggag agtttgacga cgatcttctc gaagccaaaa gtgctgtaca
     8821 tatcatagac taaacggata catccgttaa cttcatcgcg aatttgttct tcagtacaga
     8881 agatatgcgc gtcatcctgg gtaaatccac gcacgcgcat caggccatgc agcgaacctg
     8941 acggctcgtt acggtggcag ctaccaaact cggccatacg cagcggcaga tcgcgataag
     9001 acttcagccc ctggttgaaa atttgtacgt gacccgggca gttcatcggc ttaatgcagt
     9061 attcacggtt ctcagaagat gtggtgaaca ttgcatcttt gtagttgtcc cagtgaccgg
     9121 ttttttccca caggacacgg tccatcatga acggaccttt aacttcctga tactggtact
     9181 ctttcagttt agaacgaaca aacacttcca gttcacggaa gatggtccag ccgtcgttgt
     9241 gccagaatac catacccggc gcttcttcct gcatatggta caggtcgagc tgtttaccga
     9301 ttttacggtg gtcgcgtttc gcggcttctt ccaggcgctg caggtaagcg ttaagtgctt
     9361 ttttgtctgc ccacgccgta ccgtaaatac gttgcaacat tttgttgttg ctgtcgccac
     9421 gccagtaagc ccctgccgtt ttcattagtt tgaaatgatg gcagaaacgc atgttcggta
     9481 cgtgcggacc gcggcacata tcgacatatt cttcatggaa gtacagacct ggcttgtcat
     9541 catgggcgat gttttcgtca agaatggaga ctttgtagct ctccccacgg ttggcgaaag
     9601 tttcacgcgc ttcgtgccag ctgactttct tcttaatgac gtcgtagttt ttctcagcaa
     9661 gctcatgcat ccgcttctcg agtgcttcga catcttcctg ggttaacgtg cggtcaagat
     9721 caacgtcgta ataaaaaccg ttgtcaataa ccgggccgat tgccattttg gtatgcggcc
     9781 aaagttgttt aatcgcatgc cctaacaggt gcgcacagga gtgacgaatg atctccagac
     9841 cttcttcgtc tttggcggta atgatcgaca gttgtgcgtc gttttcaatc agatcgcaag
     9901 catcaaccag ttcgccatta acgcgccctg cgatacaggc tttcgccaga cctggaccaa
     9961 tgtccagcgc aacatccatg gggcttacag cgtggtcgta atggcgttgg ctgccatcag
    10021 gaagagttat aacaggcatt ttatatcctt aatttgcagt ggtgacccac acgaaagatc
    10081 acatacaaag aaaaatttgt ttattaacag ttcattgcga aaccatctag ccaacaaatg
    10141 ctaaattggt tcgcaatcag gtacacaaat gaaagacatt aacctctcac cttccacccg
    10201 gttgaacgat gttaacacta aaaaaaggga gattgtacct ttccgtttca cattgattcg
    10261 attcgcaatt cgtttgtagt gagtacacca gcaaaacgct taaagaacca ctgataacat
    10321 cacggtgcgt ctgaaaggtt tactgcgagc ggatttacag cagcataaac tgaatccagc
    10381 aaagttcgtt gcgtgatttg gcggtagtat tttaaacgct catgagatta atgacgaagt
    10441 ggtcatatca caatgataaa agtgacacaa ttcttatagc aatttttcgt gcacatttcg
    10501 ttctggcgat aataattaat catcatcctc aaaacctcca gacatatata aggcgaatag
    10561 attatgagtc aaaacgatat cattatcaga acccattata agtctcctca tagattgcac
    10621 atcgatagcg acataccaac gccttcatca gagcctatta atcaatttgc gcgccaactc
    10681 atcaccctac ttgatacctc tgacttaagt tcgatgctgt catactgtgt tactcaggaa
    10741 tttaccgcaa actgtcgaaa aatttcacaa aattgttatt ccactgccct ttttatcatt
    10801 aactttgcca cttcacccat ccatgcagaa aatatactca ttacattaca ctataaaaaa
    10861 gaaatcattc ccttatttct ggaaaccacg cctattaagg ctaaccattt gcgaagcata
    10921 ctggattata ttgaacagga acagttaact gccgaagatc gtaaccattg tatgaaactg
    10981 tctaaaaaaa tccatagaga aaaaactata caaccaacag taaatctcaa tggtagtgca
    11041 tttttttcgc aatctccttc tgacgctatt ttttgtcgcc atctgtcatt gcaatacgcc
    11101 cttgattcat tgagaaatgg aaaaggcaaa gtcaacctga ttaaacacta ctcctccgtt
    11161 gaatccatac agcagcatgt ccccttagtc cgggacgcgg agttcagagc attacttcgc
    11221 catcctcctg cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc tttagatatt
    11281 ttcttttgtc gaatgatggc aaacaatgtc agtcatatgt ccgcgatttt atatatagac
    11341 aatcatactt tgtcagtaag gctacgaata aagcagtcag tgtatggaca attaaattat
    11401 gttgtgtctg tttacgaccc gaacgatacc aacgttgccg tcagaggcac ccacagggca
    11461 gcacggggct ttctctcgct tgataagttc atcagttcag ctcccgatgc tcagacctgg
    11521 gctgataggt atgttcgcaa ctgtgcaatt gctattcttc ctctattacc tgagggagtt
    11581 ccagttgcta ttttagcggg tatcacgaca cgaatgccat ttgcccctat acatccatcg
    11641 gcaatgttgt taataatggc cacaggccag actcaatagc ttattacact attcaaacag
    11701 ttgcccatac tccctgaaaa agaaatcatt gaaataataa ctgcgcagaa tagcgttggt
    11761 acacctgctt tatttctggc tatgatgaac ggacaaactg acaacgtaaa aatatttatg
    11821 caagaaattc agtcactggt atacaatcat atcattcatg aagataatct ggttaaatta
    11881 ctgcaaacta aaagtgctaa cgaaacacct ggactctata tctccatgtt gtatggattc
    11941 gatgaaataa tcgatatctt tctgaatgca ttaaccactc caatagcaca agaactttta
    12001 aacaaaaaaa tggtgatgag tattttagcc atgaaaatac atgatggtga gccaggatta
    12061 tacgccgcaa tggaaaataa tcaccctttg tgtgtcacac ggttcctctc taaaattaat
    12121 ggcatcgcct ttaaatacaa gttgagcaaa gctaacatca tggatttatt aaaaggagct
    12181 acagcacagg gaacccctgc attatacatc gccatgagca agggtaatga agacgtcgtg
    12241 ttatcttata tatcgacgct gggtgctttt gcaaaaaaac attcttttag tcaacatcag
    12301 ttatttacac tattggccgc taaaaatcat gacaacatgt cagctgttca tatagccatt
    12361 catcataagc attataaaac tgtagaaaca tattatgctg ctataaatgt catcagccaa
    12421 agcctgagtt ttagtgctga tgaaataaag acgtatttat aaccagctat ttacgatata
    12481 tatcgataca taattttatt tttttcataa aacgcagaaa cagtgaccat gtgcacaacg
    12541 ccacccgacg gctgtaataa caaaaaatcc cttgatgcct atcccttttg ttacactccg
    12601 ttatcacgca caagagatat gcaggacact ggtatgccga ctaaacgctt tgataaaaaa
    12661 cactggaaga tggtggtggt gctactggca atctgtggcg ctatgttgtt gctacgttgg
    12721 gcagcaatga tttggggctg agcagtgaca aacgaagaca gccagaccgc ataacacggt
    12781 ccggcagata attttacatg ctgtagccca gcgataaggt tgtccgacga tcggtatgtt
    12841 ccggtgcaga ctctggaggt tcagagttcc atgtgacgtt gtaagccacc ttcagtccaa
    12901 aatgttcatt aatagcaaca tttaatgcgc tttcggagtt cagcgttgta tcttccgcgc
    12961 caaagaccga aacaccctgc gtaaatttag cgttgtcagt caactgccat gcataagccc
    13021 cggaggcata gcccagcggc tgggtttcac tggcattgtc ggtatatttg tcgtaacgca
    13081 cacctgggcc gaattcaaag cggaaactgt gtaccgggcc attcaaaaac tggcgaccat
    13141 aacccgcggt caacacatcg cgctctcgat agccgttata acggtctgtc agccagcttg
    13201 cctgaccaaa taaatagtca taatcagtta aattaaaacg gctacgcccg cccgccgcat
    13261 atttttctga agaacgctca tcattagaag aagtattact ggcgttcccc cacagcgacc
    13321 aggcagtggt ttgtccatac caggtcatgg tggtgtcagc cgtaagtgag gagcttttcg
    13381 tattgcctga ttgtgcaaga tatcctgcgt tcagattacc ttcgaaaggt tttttagcgc
    13441 tggcaggatc gtccatgaca gtaaaaacgg aatcatcggc agctgcattc agtgacgcaa
    13501 acatgccccc cgccaacata acgatggcag gaactgtctt caaaagcttc atttatcaag
    13561 agtccgtaca acaaaaaaag agaccatcgc ggtcccggaa actttcttaa ggatcaaaga
    13621 ttagcgtccc tggaaaggta acgaattata aaaaggcgcg aataacttag caatgtattc
    13681 ttatttcatt ttttgaataa gcatgtggcg aaaacagatt tttatttata tatatttatc
    13741 tgcaaaattt taaataaagc tccaataaat catattgtta atttcttcac tttccgctga
    13801 ttcggtgcca gactgaaatc agcctatagg aggaaatgat ggtacgtatc tatacgttga
    13861 cacttgcgcc ctctctcgat agcgcaacaa ttaccccgca aatttatccc gaaggaaaac
    13921 tgcgctgtac cgcaccggtg ttcgaacccg ggggcggcgg catcaacgtc gcccgcgcca
    13981 ttgcccatct tggaggcagt gccacagcga tcttcccggc gggtggcgcg accggcgaac
    14041 acctggtttc actgttggcg gatgaaaatg tccccgtcgc tactgtagaa gccaaagact
    14101 ggacccggca gaatttacat gtacatgtgg aagcaagcgg tgagcagtat cgttttgtta
    14161 tgccaggcgc ggcattaaat gaagatgagt ttcgccagct tgaagagcaa gttctggaaa
    14221 ttgaatccgg ggccatcctg gtcataagcg gaagcctgcc gccaggtgtg aagctggaaa
    14281 aattaaccca actgatttcc gcttcgcaaa aacaagggat ccgctgcatc gtcgacagtt
    14341 ctggcgaagc gttaagtgca gcactggcaa ttggtaacat cgagttggtt aagcctaacc
    14401 aaaaagaact cagtgcgctg gtgaatcgcg aactcaccca gccggacgat gtccgcaaag
    14461 ccgcgcagga aatcgttaat agcggcaagg ccaaacgggt tgtcgtttcc cttggtccac
    14521 aaggagcgct gggtgttgat agtgaaaact gcattcaggt ggtgccacca ccggtgaaaa
    14581 gccagagtac cgttggcgct ggtgacagca tggtcggcgc gatgacactg aaactggcag
    14641 aaaatgcctc tcttgaagag atggtccgtt ttggcgtagc tgcggggagt gcagccacac
    14701 tcaatcaggg aacacgtctg tgctcccatg acgatacgca aaaaatttac gcttaccttt
    14761 cccgctaaca aaaacattcc cccagcattg ggggaatcac caccaacctg tcggcaacgc
    14821 gtttctccga ctttgctcaa aagtcatgtg ataacaaagg ggtgaactat ggccagtggc
    14881 gatcttgtcc gttatgtcat aaccgtcatg ttgcatgagg atacattgac tgaaattaac
    14941 gagttgaata attacctgac tcgcgacggt tttttgctca ccatgacgga tgatgaggga
    15001 aatatccatg agctggggac taacactttt ggacttatca gtacccaaag tgaagaagaa
    15061 attagagaac tggtttcggg gcttacccaa agtgcaaccg gcaaagatcc tgaaatcacc
    15121 atcacgactt gggaggaatg gaatagcaac agaaaataaa tggtttttgg gcaataatca
    15181 gtctgtggtg tgcgttagct cgtgttttta caccgcattc ttgcgctaac cttatgatct
    15241 ggcagacaac atgggagaga catcatgtgg caggcaatca gtcgtctttt gagcgagcag
    15301 ttaggtgaag gcgaaatcga actgcgtaat gaactgcctg gcggagaagt ccatgccgca
    15361 tggcatttgc gctatgcagg acatgacttt ttcgtcaaat gtgatgaaag ggaactgctt
    15421 cccggtttta ccgccgaagc cgaccaactg gagttactgt cgcgtagtaa aaccgtcacc
    15481 gtgcctaagg tttgggcagt aggcgctgac cgtgactaca gttttctggt gatggattat
    15541 ctcccacctc gtccgctgga tgcgcatagc gcatttattc ttggtcagca aattgcgcgt
    15601 ttacatcaat ggagtgacca accacaattt ggcctcgatt ttgataactc gctctccaca
    15661 actccacagc ccaacacctg gcaacgtcgc tggtcaacgt tttttgctga acaacggatt
    15721 ggctggcagt tggaactggc agcagagaaa gggatcgctt tcggcaatat cgacgccatc
    15781 gtcgagcata ttcagcagcg tctggcctcg catcaactgc agccttctct gttgcacggc
    15841 gatttatggt ccggcaactg tgcgctgggt ccggatggcc cgtacatttt cgaccctgcc
    15901 tgctactggg gtgaccgaga gtgcgacctg gcgatgttac cgctgcatac tgaacagccg
    15961 ccacaaatct atgacggcta tcagtcagta tccccgctac ctgccgattt ccttgaacgt
    16021 caaccggttt accaactcta cacgctgtta aatcgtgcaa gattatttgg cggtcagcat
    16081 ttggttattg ctcagcagtc attggataga ttattagcag catgatatgg gttgaggata
    16141 atggccgctc cgtgcggcct tttgattaaa taaacccaag cagagaaaag aacacataac
    16201 ctgcagcaat acaaataacc ggcaggatat atagtgagaa aaactgcagg aaaatagtat
    16261 ggtgcggaac aacaatacgg gactcaattt gttcacgcgt tagcccctct tcccctttgg
    16321 ctttttccag aatgagttga tcttcaacac cttcccgcaa gaagcgcgcc tggcgactca
    16381 tccgggcacc ggaatcctgc aacgccagtc cgacaaaaat caggatgaaa atcacccaga
    16441 acataacgtt cacgccacca ttaaaattag gggtcgggga gttataccag aacagattca
    16501 aaaaaggcgt attcgcctgc atcatatcga tcatgacatg agtaaagtcg agcatgaccg
    16561 cattaatgcc ttcctggttt tcctgccggg tattcataaa tttcagcaaa gaaatcaatg
    16621 tggagatcaa tgcaggtata aaaatcaccc aacccaaaat ccttttcaaa acagcaatgc
    16681 gtccagcttg ttgatacgtc atgagttctc cttgattaag acgcgtcgtt tcacttagtt
    16741 tacctgtaga tatctgtttt cgcccattct ttaaaggcga tatgataggc gcttaatcat
    16801 aagcacggct taatacctta cacataatgc tctaaaggag aggttgtaat gtcaaccccg
    16861 cgtcagattc ttgctgcaat ttttgatatg gatggattac ttatcgactc agaaccttta
    16921 tgggatcgag ccgaactgga tgtgatggca agcctggggg tggatatctc ccgtcgtaac
    16981 gagctgccgg acaccttagg tttacgcatc gatatggtgg tcgatctttg gtacgcccgg
    17041 caaccgtgga atgggccaag ccgtcaggaa gtagtagaac gggttattgc ccgtgccatt
    17101 tcactggttg aagagacacg tccattatta ccaggcgtgc gcgaagctgt tgcgttatgc
    17161 aaagaacaag gtttattggt gggactggcc tccgcgtcac cactacatat gctggaaaaa
    17221 gtgttgacca tgtttgactt acgcgacagt ttcgatgccc tcgcctcggc tgaaaaactg
    17281 ccttacagca agccgcatcc gcaagtatat ctcgactgcg cagcaaaact gggcgttgac
    17341 ccactgacct gcgtggcgct ggaagactcg gtaaatggca tgatcgcttc taaagcagcc
    17401 cgcatgcgtt ccatcgtcgt tcctgcgcca gaagcgcaaa atgatccacg ttttgtatta
    17461 gcaaacgtca aactctcatc gctgacagaa ctcaccgcaa aagaccttct cggttaatga
    17521 ccaggggcag tgatcacgct gcccctggtt ctttatctga atttgcattc aactgacgga
    17581 ttaatcgtca atttaagaga aagagttaca ccgtcaccac ttccgtgcac tgtataaaaa
    17641 tcctatactg tacgtatcga cagtttagtg agttttatca tgacggcgga aggtcacctt
    17701 ctcttttcta ttgcttgtgc ggtatttgcc aaaaatgccg agctgacgcc cgtgctggca
    17761 cagggtgact ggtggcatat tgtcccttcc gcaatcctga cgtgtttgtt accggacatc
    17821 gatcacccaa agtcgtttct tgggcagcga ttaaaatgga tatcaaaacc gatcgcccgc
    17881 gcttttgggc atcgtggttt tacccacagt ctgctggcgg tatttgcgct gctggcaacc
    17941 ttttacctta aggttccgga aggctggttc attccggctg atgcgctaca aggaatggtg
    18001 ctgggttatt tgagccacat acttgccgat atgctgacac ccgccggtgt tcccctgctc
    18061 tggccatgcc gctggcgttt ccgcttgcct atcctggttc cccaaaaggg caaccaactg
    18121 gaacgtttta tctgcatggc attatttgtc tggtcggtat ggatgcccca ttcattaccc
    18181 gagaacagcg ctgttcgttg gtcatcgcaa atgatcaata ccttgcagat ccagtttcat
    18241 cggcttatta agcatcaggt tgaatactaa aaaggcaaaa atcacctttc tggaataagc
    18301 aattccattt gaatataaga gccagctcac agttctgtta atcttgcgcc aacactatga
    18361 ctgctacgca gtgatagaaa taataagatc aggagaacgg ggatgaactt tccattaatt
    18421 gcgaacatcg tggtgttcgt tgtactgctg tttgcgctgg ctcagacccg ccataaacag
    18481 tggagtctgg cgaaaaaagt gctggtgggt ctggtgatgg gtgtggtttt tggccttgcc
    18541 ctgcatacca tttatggttc tgacagccag gtacttaaag attctgtaca gtggtttaac
    18601 atcgttggta acggctatgt tcaactgctg caaatgatcg ttatgccgtt agtcttcgcc
    18661 tctattctga gcgcggttgc ccgtctgcat aacgcatctc agttaggcaa aatcagtttt
    18721 ctgaccatcg gtacgctttt gtttaccacg ctgattgcgg cgctggtcgg tgtgctggtc
    18781 accaacctgt ttggtttgac ggctgaaggt ctggttcagg gtggtgcaga aactgcacgt
    18841 ctgaacgcca ttgaaagtaa ctatgttggt aaagtctctg atctgagcgt tccgcagctg
    18901 gtcttgtcct ttatcccgaa aaacccgttt gccgatctta ccggagccaa tccgacgtca
    18961 attatcagcg tggtaatttt tgccgcattc ctcggcgtag ctgcattgaa actgctgaag
    19021 gatgatgcgc cgaaaggtga acgcgtctta gccgctatcg ataccctaca aagctgggtg
    19081 atgaaactgg ttcgccttgt catgcagttg accccttacg gcgttctggc tctaatgacc
    19141 aaagtggttg caggttctaa cttgcaagac atcatcaaac tgggaagttt cgttgtcgcg
    19201 tcctacctcg gtctgctgat tatgtttgca gtgcatggca ttctgctggg cattaatggc
    19261 gtgagtccgc tgaagtactt ccgtaaggta tggcctgtgc tgacgtttgc ctttaccagc
    19321 cgttccagtg ctgcgtctat cccactgaat gtggaagcac aaacgcgtcg tctgggcgtt
    19381 cctgaatcca tcgccagttt cgccgcctct ttcggtgcaa ccattggtca gaacggctgc
    19441 gccggtttgt atccggcaat gctggcggtg atggttgcgc ctacggttgg cattaacccg
    19501 ctggacccga tgtggattgc gacgctggtc ggtattgtta ccgttagttc cgcaggcgtt
    19561 gccggtgtcg gtggtggtgc aactttcgcc gcactgattg tgctgcctgc gatgggcctg
    19621 ccagtaaccc tggtggcgct gttaatctcc gttgaaccgc ttatcgacat gggccgtacg
    19681 gcgctaaacg ttagtggctc gatgacagct ggcacgctga ccagccagtg gctgaagcaa
    19741 accgataaag ccattctgga tagcgaagac gacgccgaac tggcacaccg ttaattctta
    19801 tgctggcaac ggtccgtttt gtataggggc cgttgcttta ctttttaaat gttccatcgc
    19861 ggcgatgcat actgactcat atttaaacag atcgtcagcg aaaaaaatgt ccaacttatc
    19921 aatacattcc tggctcaatg aatgtgcctg tagtgagaag cttaaaacct catacccgag
    19981 acgttttcta agtgaactat ctggaatact cataatatct tcaagcacac cacttcccat
    20041 tcccggaaga tttagtggat taaaaagaag attgatatta agcattgata ttatcgaagg
    20101 aaactttagg agactctcat tttttttgaa tcgcagcaaa aatgctcccg gcaggatgta
    20161 tataattttt ttcatagtat atagtcctta aataatgaat aaccaaccta ttttttatat
    20221 tgatcaaatt cgtcattttt tcgggtcgga attcacaggt aaaatccatc ccaacaaaat
    20281 tctcattaat atctcttatc cgaaactcac tgccataaag attattaatt atcactctat
    20341 atttaacatt gtcctgcaga tacgtatcaa aactaagtgc tggagctcct ggctttgagg
    20401 catcatagtt aatgttagac atataggcaa tcatacgatt gaagttcgac tctgctattt
    20461 catcgcagtc acattgaccc gttgatataa agaaagtgag tgccgccatg gtagactcaa
    20521 gcctttcttt aaaagagtcg aaagccaggc ttttaatatt taaatcacca taattactct
    20581 gtattaagtt tgtagaaaac atctcccgcc ccatattgtt aacaaaatta ttatctcatt
    20641 taaatctaag tcacttacaa tataagttta agagcgacgc cacaggatga actatcaaaa
    20701 atagctcatc atgattagca aaacttaacc attttaaaat aaataaacaa ttaaagaaaa
    20761 aagatcactt atttatagca atagctcgtc aaaggcagct ttttgttaca ggtggtttga
    20821 atgaatgtag caacgaaatg cagaatttca ggtcatgtaa ctcccggcaa aaccgggaag
    20881 tatgtaatcc ttactcagtc acttcccctt cctggcggat ctgatttgcc caacgttggg
    20941 cagattcagg cacagtaaac gccggtgagc gcagaaatga ctctcccatc agtacaaacg
    21001 caacatattt gccacgcagc atccagacat cacgaaacga atccatcttt atcgcatgtt
    21061 ctggcggcgc gggttccgtg cgtgggacat agctaataat ctggcggttt tgctggcgga
    21121 gcggtttctt cattactggc ttcactaaac gcatattaaa aattagaaaa actgtagttt
    21181 agccgattta gcccctgtac gtcccgcttt gcgtgtattt cataacaccg tttccagaat
    21241 agtctccgaa gcgggatctg gctggtggtc tatagttaga gagttttttg accaaaacag
    21301 cggccctttc agtaataaat taaggagacg agttcaatgt cgcaacataa cgaaaagaac
    21361 ctacatcagc accagtcacc actacacgat tccagcgaag cgaaaccggg gatggactca
    21421 ctggcacctg aggacggatc tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa
    21481 cctaccgccc ctgggagcct gaaagcccct gatacacgta acgaaaaact taattctctg
    21541 gaagacgtac gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc
    21601 gccgatgatc aaaactcact gcgtgccggt aaccgtggtc caacgctgct ggaagatttt
    21661 attctgcgcg agaaaatcac ccactttgac catgagcgca tcccggaacg tattgttcat
    21721 gcacgcggat cagcagctca cggttatttc cagccatata aaagcttaag cgatatcacc
    21781 aaagcggatt tcctctcaga tccgaacaaa atcaccccag tatttgttag gttctctacc
    21841 gttcagggtg gcgctggctc tgcagatacc gtgcgtgata tccgtggttt tgccaccaag
    21901 ttctatactg aagagggtat ttttgacctc gttggcaata acacgccaat cttctttatc
    21961 caggatgcgc ataaattccc cgattttgtt catgcggtaa aaccagaacc gcactgggca
    22021 attccacaag ggcaaagtgc ccacgatact ttctgggatt atgtttctct gcaacctgaa
    22081 actctgcaca acgtgatgtg ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc
    22141 atggaaggct tcggtattca caccttccgc ctgattaatg ccgaagggaa ggcaacgttt
    22201 gtacgtttcc actggaaacc actggcaggt aaagcctcac tcgtttggga tgaagcacaa
    22261 aaactcaccg gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc cattgaagca
    22321 ggcgattttc cggaatacga actgggcttc cagttgattc ctgaagaaga tgaattcaag
    22381 ttcgacttcg atcttctcga tccaaccaaa ctgatcccgg aagaactggt gcccgttcag
    22441 cgtgtcggca aaatggtgct caatcgcaat ccggataact tctttgccga aaacgaacaa
    22501 gcagcattcc atccaggtca tattgttcct ggtctggact tcaccaacga tccgctgttg
    22561 cagggacgtt tgttctccta taccgataca caaatcagtc gtcttggtgg gccgaatttc
    22621 catgagattc cgattaaccg tccgacctgc ccttaccaca acttccagcg tgatggcatg
    22681 catcgtatgg ggatcgacac taacccggcg aattacgaac cgaactcgat caacgataac
    22741 tggccgcgcg aaacaccgcc gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc
    22801 gtggaaggaa ataaagttcg tgagcgcagt ccatcgtttg gcgaatatta ttcccatccg
    22861 cgtctgttct ggctaagtca gacgccattc gagcagcgcc atattgtcga tggtttcagt
    22921 tttgagttaa gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga ccagctggcg
    22981 catattgatc tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga actgactgac
    23041 gaccagctga atatcacccc acctccggac gtcaacggtc tgaaaaagga tccatcctta
    23101 agtttgtacg ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat
    23161 gatgaagtga gatcggcaga ccttctggcc attctcaagg ccctgaaggc caaaggcgtt
    23221 catgccaaac tgctctactc ccgaatgggt gaagtgactg cggatgacgg tacggtgttg
    23281 cctatagccg ctacctttgc cggtgcacct tcgctgacgg tcgatgcggt cattgtccct
    23341 tgcggcaata tcgcggatat cgctgacaac ggcgatgcca actactacct gatggaagcc
    23401 tacaaacacc ttaaaccgat tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc
    23461 aaggtcgctg accagggtga agaagggatt gtggaagctg acagcgctga cggtagtttt
    23521 atggatgaac tgctaacgct gatggcagca caccgcgtgt ggtcacgcat tcctaagatt
    23581 gacaaaattc ctgcctgatg ggaggcggcg caattgcgcc gcctcaaatg atttacatag
    23641 tgcgctttgt ttatgccgga tgcggcgtga acgccttatc cggcctacaa aactgtgcaa
    23701 attcaatata ttgcaggaaa cacgtaggcc tgataagcga agcgcatcag gcagttttgc
    23761 gtttgtcagc agtctcaagc ggcgcagtta cgccgccttt gtaggaatta atcgccggat
    23821 gcaaggttca cgccgcatct ggcaaacatc ctcacttaca catcccgata actccccaac
    23881 cgataaccac gctgagcgat agcacctttc aacgacgctg atgtcaacac atccagctcc
    23941 gttaagcgtg ggaaacagta agcactctga cggatagtat tatcgataaa cgccggatga
    24001 cacatcacct ccagcgaacg atcaccccga tggcttgcgt catccagcac ttgcaggaac
    24061 agtgcctcgc taatctcttc accataaaac gcgctactga atccctgaga actgcgcaga
    24121 ttagccggta aatcaccaga gttagatagc ggctgacggt cgatgcgcaa cgcaatcccc
    24181 tcttcagccg caaacctggc aacaatcggg aaaatctgcg ggaacatatg cacatgatga
    24241 tggctatcaa gatgcgtagg tttgcgtcca aatagctcaa tgaaacgcaa atactgactg
    24301 gcaagctcct gagtaatttc ttccagcggt aaagcatctt cttctgccaa ctgccagatc
    24361 cattttccca gcacaccatc gcgggttaac cccggcatag ctgtcagtgg cttgcccata
    24421 gtaaggacaa agtacatccc tatggccaga ctcggttcat cacgactcaa ctgcaccgca
    24481 tggtcaatag cctgcccatt caccagcgcc gtcgtcgatg tgacaatccc attgcgacag
    24541 gcctcgataa tgccgtagtt ctgacctttg cttaagccaa aatcatcggc attaacaatc
    24601 agtaagcgtt ccataatcag cctcggttaa tgtgcttttt taagctctgc gatgcagtcg
    24661 gcaaaatttg gcagccattt ctcgtgcgcc agaatcatct cgcgtgccag cagctcagca
    24721 tcgcgatcgg aatgcaccaa cggactaagg tttagcgcca gtaacacatc gttaaattct
    24781 ccgctaagtg cggcgttgct ggcagcaatc tcgaagcctt taatggtgtg aatcagcccc
    24841 atcactttat catcgaaatg cgtaatgcgc ggatgtggcg tcgcgccatc gcgccccagc
    24901 ttacaggtca tttctaccgc ccagtctgcc ggaatattat caatatgccc atgatgcggg
    24961 atattaacgt aatgttcagc ttgcttgtcg ttgtagatag cgttgatcac ttcgcacgct
    25021 gcatcagagt aataagcccc accgcgctgt tccagttctt tcggcttaac tttcaactcc
    25081 gggtttttat acagctcaaa aagttgtttc tcgactttct gtactacctg cgctcgtgcg
    25141 ccgcctttgt agtattcgcc catttcaata gccagcatct ctttttgctt gaagtaataa
    25201 agcaaataag aacacggcaa cagattcaga gaacgaatta agccttcgct aaatggcaga
    25261 tcgaaaatat ttttaacgcc agatgctttt aactgcccgg atgccacacc atcaagcaat
    25321 tcggcaaagc gcgacttgcc atttaccagc acatccttaa tgaacaccat atggttgagg
    25381 ccgaacaaat cgatagataa atcatcgctg tctttcagca tcagaacatc gtgaataaac
    25441 atcttcatgc cgatcggaat attacacacg ccgataaagc gtttaaatcc ggtatgacga
    25501 taaacggctt cagtaaccat tcccgccggg ttagtgaagt taatcaccca tgcattcgga
    25561 caaagttctt cgacatcttt tacgatgtca aaaatcaccg gaatggtacg cagaccttta
    25621 aacagaccgc ccgcgccgtt ggtttcctgg ccaagataac catgacttaa tggaatacgt
    25681 tcatccagtt cgcgcgccgg taattggcct acgcgcagtt gggtagtaac gaaatcagca
    25741 tctttcaatg cttcgcggcg atccagcgtt ttataaagct tcatcgggac gccagcgtta
    25801 tcaatcatcc gttggcagag ttcaaaaatg atatccagtt tctctttacc atcttcgaca
    25861 tccaccagcc ataattcgct gaccggcaat tcgtgataac gcttaataaa tccttccagt
    25921 aactccgggg tatagctgct cccgccacca atagtgacga cttttaattt ctggctcata
    25981 atttctccct tcagtacaga atactgatat ctggcatatc tgcccccccg gacataaata
    26041 atccagcaac aggacagata tgtgaattgt caggtataac gacttactga ttaaattcag
    26101 tcaatttctt acgatagctc ttaggcgtaa aggacgttaa tttcttaaac gttttaataa
    26161 acaaactcgg gctactataa ccggcctcaa acgcaatatc cgtcacggaa tagttggtca
    26221 tttccagttg ttttttggca aaattaatac ggatctcatt aataatctgc attggtgttt
    26281 tgccataata tcgttgagtc gctcgcgtca aatattcctg tgatttggct gacaacgtca
    26341 ccatattctc cagcgccgat tcactaaact gctctttatc atgcatcttt tctaccgtac
    26401 ttttcagcca ctgcggtaca tcatcaatca cctgttcttc gcggtaatga cgtaaacggt
    26461 taatgacata aaaagtaacc atctcaacaa actcttccag ccctgtttcg cggaaattca
    26521 atgaagaaat cactgtttcc acataggtga gaaaagcgtt attggtccgg tatacctgcg
    26581 aagcgacaaa gcaataaggc aacaacggca ggtaatgctg ctcaaaaaag cgtttactga
    26641 tcccaacgtt caatatacgc gtggcaccaa actcataaaa actttgatgg tgcgaaccta
    26701 acggaataaa aacaaaatcg ccccgttcca gtaacacgcg cttaccgtta atctcctgga
    26761 aataacgccc ggttaatacc agagtaaatt cataatagtc gtgctgatgc agcccgctga
    26821 tactctcagt tttgttatag ataaacacat ggaagttttt gccattaaac aactgctgtt
    26881 ctcgggcagt ggcaatttcc ggcgcgttaa tcactggctg catcatcgac tccttatgcc
    26941 ttcagttttt catgaagctc aattaattca gtaatcagtt cacgcgcaag catggacgtc
    27001 attaaatgat cctgagcgtg gaccagcacc agactcactt tcatcttacc ttcgcccgca
    27061 tcgccttcaa tcagtttcgt ctgtaccaga tgcgcttcat tcaatgccat ccgtgactga
    27121 tccatcatgg cttttgctgc agcaaaatcg ccctgcttcg cctgtttcag tgccgcatac
    27181 gccaggctgc gcgcttgtcc ggagttgatg ataagcccca tcaccacttc ttccagctct
    27241 tcagcttccg tttgcgtatc gggaatgtta tcgagatcca tcatacatcg ttcctctcta
    27301 ttcttaccgg cacgattacc cgtaccggca tcgattaaaa tttcagggcg ttagcgatat
    27361 cttcttcgcg ctcttcttta tcaatcgcgt tttgtgcttt gttagccacc acaacaaagg
    27421 gcagataaat taacgttgcg ataccaaggt tgaagagtgc aaccagcaat gcggcgacgc
    27481 taccgttggt gttaaagaag gctcccagac cggttggcat ggtccacggt gcaatattgg
    27541 tcaccggagg aatgatgccc atgtagtacg ctgcgagggt gattgccgcc agaatcggtt
    27601 gtaccagtac aaacgggata aacatcaccg ggttcatgat aattggcaga ccaaacagaa
    27661 tcggttcgtt aatctggaag atgccggacg gcagcgccag ttttgccacc tgacgataat
    27721 cagcacggcg agaggcgata aagatagcca ggatcaggcc taaagtcgca ccactgcccc
    27781 caaggaaaat aaaggagtcc agcatcggct tggcccagat atggaaggtc ttaccggctg
    27841 ccagcgccgc ttcgacggaa ccatattgct gataggtcgc gatattttcc agcgcccacg
    27901 gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc gccatgaata ccgaagaacc
    27961 agagcagtgg aacaaagatc acataggccc agcccaccac gctacccaac gatgccagtg
    28021 gggttgagat ggtatccata atgatctgat ggaagttggt gccccaggta ttcaacgccc
    28081 aggcaataat ccccatcacg gaaagaataa taaagccggg aattaatgcc gagaaggaac
    28141 gcgatactga agcaggtacg ctatcgggca atttaatgac ccaattgcgg cggacaataa
    28201 aggtaaacat ttctgccacc accaggccaa taataatccc ggagatgata ttcgctccac
    28261 ctaaccagtt tgcgccaacc acataggcct caccgacact atatggggtg acggtcataa
    28321 atgctgcaac ggataacaac ccagccgcca gcgcatcgac tttacgctct tctgccagcg
    28381 ccatgccaat aaagaacggt gccattaaag acattatccc taatgttccg ttatatacgt
    28441 tgccgccaat acctttcagg ccattaagtg tttcaatggt tgaggcgtcg aggcgaatac
    28501 ctaaggaata aaaaaacgcc ccctccccaa agcttagaaa aacgttgtta attaatacaa
    28561 acatggcccc cgcaagggtt aacggcatta agcgaataaa gccatttttg attgcattaa
    28621 cgtgtggctg ctttcctatt ttaactgcaa aagggaggag taccttttca agcgatgcaa
    28681 taacattact catagaaaaa tacccttaaa aaccgcaatt taaatattgc ggtattgatt
    28741 tatgaaataa ctctttgacg ggaaaattta aaataaatta atttgctgcg gcttttttaa
    28801 tcgctgcaac cgcagcctta agcacgccta aaccatcgac tttgccataa agcagcgagt
    28861 caattacttc aaccggtttg ttgggtaaca aacgctggat ttcgggcaac atataagcaa
    28921 tctgcggccc taataacacg acatcggcat tctgaccttt ttcaccagcc agtgtttccg
    28981 gaaatgcttc aataatgacc ggaacttcat atttttctgc ctgtgcgcgc atttttgata
    29041 ccagtaaaga ggtagacatg cccgcagaac aaaacagata aatgtgtttc ttttccataa
    29101 aactgccctc atcgacgatt atctgtcagc cagacactcc gcaagcctta acctgcttcc
    29161 atgctctggg taacttgcga aaccaacatg actttttttg tggctgaaag gagtatacgg
    29221 tatagacctg agcaatagta tttctttgac cttataaatt gtttctcctt gacctgtggt
    29281 tatgaccctc ttcacatttc gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa
    29341 aaaaaaccgg cgcaatggcc ggtttccgtt gttactcaag ctttcagacg aattgattac
    29401 ttcgcagcct gtggatcagt gtcgtattca gcacaggtct gataaccgga gttgatgaca
    29461 tgtccggtat catctaacgc gacaaagtag gtttctgctt taccatcacg ttgacccagg
    29521 atgtaggtct ggcaagtacc acgagcatgg atcatgctca cttcagacga aggtttaccc
    29581 gcaatttgtg caacctgcgc ccggctcatg ccttttttga cgtctttcac cacaggctgt
    29641 acaaactggt ctttagtacg atcataagcc gtacaacccg ccagcatggt taataccgcc
    29701 gctgcactca gaattcctgc catattcttg ttcatattcc gtcctcttgt ttatcagcgt
    29761 gttagataag cctggaatac attgggcgct ttttcaagcc cgcgaacgaa acggctccgc
    29821 tttcagagga ttcctgtatg acgttttaac caccattcag cccgctgtcg cttgtcgttt
    29881 cagtggcaac gggttagctt taaggaagtt ttgtcttttc tgtctggagg ggttcaatga
    29941 cattgcaaca acaaataata aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg
    30001 aaattcgtcg tagtatcgat tttctgaaaa gctacctgca aacttatccg ttcattaaat
    30061 cactggtgct cgggatcagc ggcggtcagg actccacgct tgccggaaag ctgtgccaga
    30121 tggcgattaa tgagctgcgc ctggaaaccg gcaacgaatc actgcaattt attgccgtac
    30181 gcctgcccta tggtgttcag gccgacgaac aagattgcca ggatgccatt gcctttattc
    30241 aaccggatcg cgtattaacc gttaatatca agggcgcggt attggccagc gaacaggcat
    30301 tgcgggaagc agacattgaa ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc
    30361 ggatgaaagc acaatatagc attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc
    30421 atgcagcaga agccattacc ggattcttca ctaaatatgg tgacggcggt acagacatta
    30481 atccgctgta tcgtctcaac aaacgtcagg gtaaacagtt actggcggca ttaggttgcc
    30541 cggaacacct ttataagaaa gcgccaacgg ccgatctgga agatgatcgc ccttctctgc
    30601 cagatgaagt ggcactcggc gtgacctatg acaatatcga cgactatctg gaagggaaaa
    30661 acgtacctga acaggtcgcc agaacaatag agaactggta tctgaaaacc gaacataaac
    30721 gccgtccgcc aattaccgtt ttcgatgatt tctggaaaaa gtaataattt gcacataatg
    30781 gtttgtgcga aaaagagtaa tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc
    30841 tacaaaacta tgcaaattca atatattgca ggagtcgaat aggcctgaca ggcgtagcac
    30901 gtcagacggt gtaacctttg tcatcgaccc gcctcttttt taatcgctcc ccgcctgtta
    30961 cactggatag ataaccagca ttcggagtca acagtggtac ggcgtttaac ttctccgcgg
    31021 ctcgaatttg aagctgcggc aatttatgaa tatcccgaac atttacgttc attccttaat
    31081 gacttaccca cccgaccagg ggtgtatctg tttcatggcg aaagtgacac catgccgctc
    31141 tatatcggca aaagcgttaa tatccgcagt cgggtgcttt ctcatttacg caccccggat
    31201 gaagccgcca tgctacggca atcccggcga attagctgga tctgtaccgc aggcgaaatc
    31261 ggcgctctgc tccttgaagc gcgattaatc aaagaacaac agccgctgtt taataaacgg
    31321 ttgcgccgca atcgccagct ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg
    31381 tatgccaaag aggtggattt ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg
    31441 cgcgcagctt tgcaagcatt gcagaccatc gctgatgaac aaaaactttg ttatggcctg
    31501 ctgggactgg aaccgttaag tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc
    31561 gccggagcat gctgcggtaa agagagccat gaggaacatg cgctacgctt gcgccagtct
    31621 ctggagcgtt tgcgggtggt gtgttggcct tggcaaggtg cggtggcact gaaagaacag
    31681 cacccggaaa tgactcaata tcatattatt caaaactggc tgtggctggg ggcggttaat
    31741 tcgctggaag aagcgacaac gttaattcgg acacccgccg ggtttgatca cgacggttat
    31801 aaaattcttt gtaagccgct gctttccggt aactatgaaa ttactgaact tgatccggcg
    31861 aatgaccagc gagccagttg atttcactga acaacagttt accttgcggt tgtaaaagcc
    31921 gcagggtgtg cctgccatcg aaccagcacg cgccctggtc ggtagtgagc aatttatcgc
    31981 ccagttgcca cgccccgtta atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa
    32041 acgtacgttc ggcaattctg actttcgcct tacagacatc caggcgtgtc atgatgttga
    32101 aatccatcga catctgccct tccgtcagtt tcgcttttac cacctggtcc gctgcaaagg
    32161 caaaaggctg taacggtttt aaggtatggt taaagcggtc tgcgctttca aggagcatct
    32221 cgccgccttc cagcaacgtc actatccttt ccatgccggg aaacaaagaa aactccccat
    32281 tcgccgcgat ggatgcaatg ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag
    32341 tgcaaatttc gcgcgtttca ccggcagcat ttcgccacag attcaccgac attttacgca
    32401 tatcaaagta ttccatactc gctcccggcc cgttcttttc attgttatcg ttcaggtaac
    32461 cgtggatggt tatcggcaac tcgggtgatt tacgttagtg gtgatcaggc tttttttaca
    32521 actgtttgag aagagaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt
    32581 ggacaagacc ggcggtctta agttttttgg ctgaaagatt attcagcagt tgcaggcatt
    32641 ttaccttttg
//
LOCUS       DAQSWQ010000043        32622 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.043, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000043 DAQSWQ010000000
VERSION     DAQSWQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.043"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="R8G18_002057"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="R8G18_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEE9795754.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="R8G18_002058"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="R8G18_002058"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HEE9795755.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="R8G18_002059"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="R8G18_002059"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEE9795756.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="R8G18_002060"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="R8G18_002060"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEE9795757.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6646
                     /gene="hemX"
                     /locus_tag="R8G18_002061"
     CDS             5417..6646
                     /gene="hemX"
                     /locus_tag="R8G18_002061"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEE9795758.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATNDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            6649..7845
                     /gene="hemY"
                     /locus_tag="R8G18_002062"
     CDS             6649..7845
                     /gene="hemY"
                     /locus_tag="R8G18_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HEE9795759.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            8507..8668
                     /locus_tag="R8G18_002063"
     CDS             8507..8668
                     /locus_tag="R8G18_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795760.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(8752..9987)
                     /locus_tag="R8G18_002064"
     CDS             complement(8752..9987)
                     /locus_tag="R8G18_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HEE9795761.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10134..10210)
                     /locus_tag="R8G18_002065"
     tRNA            complement(10134..10210)
                     /locus_tag="R8G18_002065"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10174..10176),aa:Pro,seq:tgg)
     gene            complement(10253..10339)
                     /locus_tag="R8G18_002066"
     tRNA            complement(10253..10339)
                     /locus_tag="R8G18_002066"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10303..10305),aa:Leu,seq:cag)
     gene            complement(10360..10435)
                     /locus_tag="R8G18_002067"
     tRNA            complement(10360..10435)
                     /locus_tag="R8G18_002067"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10400..10402),aa:His,seq:gtg)
     gene            complement(10494..10570)
                     /locus_tag="R8G18_002068"
     tRNA            complement(10494..10570)
                     /locus_tag="R8G18_002068"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10534..10536),aa:Arg,seq:ccg)
     gene            complement(10673..12058)
                     /locus_tag="R8G18_002069"
     CDS             complement(10673..12058)
                     /locus_tag="R8G18_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEE9795762.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12249..12989)
                     /gene="rffM"
                     /locus_tag="R8G18_002070"
     CDS             complement(12249..12989)
                     /gene="rffM"
                     /locus_tag="R8G18_002070"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HEE9795763.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(12992..14344)
                     /gene="wzyE"
                     /locus_tag="R8G18_002071"
     CDS             complement(12992..14344)
                     /gene="wzyE"
                     /locus_tag="R8G18_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HEE9795764.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14341..15420)
                     /gene="rffT"
                     /locus_tag="R8G18_002072"
                     /gene_synonym="wecF"
     CDS             complement(14341..15420)
                     /gene="rffT"
                     /locus_tag="R8G18_002072"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HEE9795765.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15417..16667)
                     /gene="wzxE"
                     /locus_tag="R8G18_002073"
     CDS             complement(15417..16667)
                     /gene="wzxE"
                     /locus_tag="R8G18_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HEE9795766.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16669..17799)
                     /gene="rffA"
                     /locus_tag="R8G18_002074"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16669..17799)
                     /gene="rffA"
                     /locus_tag="R8G18_002074"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HEE9795767.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17804..18478)
                     /gene="rffC"
                     /locus_tag="R8G18_002075"
                     /gene_synonym="wecD"
     CDS             complement(17804..18478)
                     /gene="rffC"
                     /locus_tag="R8G18_002075"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HEE9795768.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18456..19337)
                     /gene="rfbA"
                     /locus_tag="R8G18_002076"
     CDS             complement(18456..19337)
                     /gene="rfbA"
                     /locus_tag="R8G18_002076"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEE9795769.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19356..20423)
                     /gene="rffG"
                     /locus_tag="R8G18_002077"
     CDS             complement(19356..20423)
                     /gene="rffG"
                     /locus_tag="R8G18_002077"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEE9795770.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20423..21685)
                     /gene="wecC"
                     /locus_tag="R8G18_002078"
     CDS             complement(20423..21685)
                     /gene="wecC"
                     /locus_tag="R8G18_002078"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HEE9795771.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21682..22812)
                     /gene="wecB"
                     /locus_tag="R8G18_002079"
     CDS             complement(21682..22812)
                     /gene="wecB"
                     /locus_tag="R8G18_002079"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEE9795772.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22868..23914)
                     /gene="wzzE"
                     /locus_tag="R8G18_002080"
     CDS             complement(22868..23914)
                     /gene="wzzE"
                     /locus_tag="R8G18_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HEE9795773.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23926..25029)
                     /gene="wecA"
                     /locus_tag="R8G18_002081"
     CDS             complement(23926..25029)
                     /gene="wecA"
                     /locus_tag="R8G18_002081"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HEE9795774.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25269..26528)
                     /gene="rho"
                     /locus_tag="R8G18_002082"
     CDS             complement(25269..26528)
                     /gene="rho"
                     /locus_tag="R8G18_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEE9795775.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26613..26714)
                     /gene="rhoL"
                     /locus_tag="R8G18_002083"
     CDS             complement(26613..26714)
                     /gene="rhoL"
                     /locus_tag="R8G18_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HEE9795776.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26855..27184)
                     /gene="trxA"
                     /locus_tag="R8G18_002084"
     CDS             complement(26855..27184)
                     /gene="trxA"
                     /locus_tag="R8G18_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEE9795777.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27315..28580
                     /gene="rhlB"
                     /locus_tag="R8G18_002085"
     CDS             27315..28580
                     /gene="rhlB"
                     /locus_tag="R8G18_002085"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HEE9795778.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28714..30198
                     /gene="gppA"
                     /locus_tag="R8G18_002086"
     CDS             28714..30198
                     /gene="gppA"
                     /locus_tag="R8G18_002086"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HEE9795779.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30245..32266)
                     /gene="rep"
                     /locus_tag="R8G18_002087"
     CDS             complement(30245..32266)
                     /gene="rep"
                     /locus_tag="R8G18_002087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEE9795780.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         8228 a         8817 c         8183 g         7394 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc aacgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca cctgcacctg cacctgcacc
     6601 tgcaccgcaa ggtgatactc cggcagccgc gccgcaagga gaataaccat gctaaaagtg
     6661 ttattactct ttgttttgct gattgcgggg atagtggttg gcccgatgat tgccggtcat
     6721 cagggttacg tgctgatcca gactgacaac tacaatatcg aaaccagcgt cacgggcctg
     6781 gcgattattt tgattctggc gatggtagtg ctgtttgcca ttgagtggct actgcggcgg
     6841 atcttccgca caggcgcaca cacccgtggg tggtttgtcg gacgtaagcg tcgccgtgcc
     6901 cgtaagcaga ccgaacaggc gctgctgaaa ctggcggaag gcgattatca gcaagttgaa
     6961 aagctgatgg cgaaaaatgc cgatcacgcg gaacaaccgg tggtgaacta tctactggct
     7021 gccgaagccg cgcaacaacg tggtgatgaa gcacgcgcca accaacatct ggaacgcgca
     7081 gcagagctgg ccggcaatga caccattccg gtagaaatca cccgtgtacg tctgcaactg
     7141 gcccgtaatg aaaaccatgc tgcacgccac ggcgtggata agctgctgga agttacgcca
     7201 cgccatccgg aagtattacg tctggcggaa caggcgtata tccgcacagg tgcatggagt
     7261 tctctgctgg atatcatccc atcaatggcg aaagcccatg ttggtgatga agaacatcgt
     7321 gcaatgctgg aacaacaggc atggattggc ctgatggatc aggcgcgtgc cgataacggt
     7381 agtgaaggtt tgcgtaactg gtggaaaaac caaagccgga aaacgcgtca tcaggtagcg
     7441 ttgcaggtgg caatggcgga acatcttatt gaatgtgacg atcatgatac tgcccagcaa
     7501 attatcatcg atggcctgaa acgccagtac gacgatcgcc tactgctgcc gattcctcga
     7561 ctgaaaacca acaatccgga acagctggaa aaagtgctgc gccagcagat caaaaacgtc
     7621 ggcgatcgcc cgctgttgtg gagcacactg ggccagtcac tgatgaagca cggagaatgg
     7681 caggaagcat cgctcgcctt ccgcgcagcg ctgaaacaac gtccggacgc ctacgattac
     7741 gcatggcttg ccgacgcgct ggacagactg cacaagccgg aagaagctgc agcgatgcgt
     7801 cgcgacggtt tgatgttaac gttgcagaac aacccaccac agtagttcct tctcacccgg
     7861 aggcaagcac ctccgggacc tttctgatgc ataaaaaaac gcctgctctt attacggagc
     7921 aggcgttaaa acaggtctgt atgacaacag tgggtgcttc actcaacgtt gtgtccatgg
     7981 tgtctgatga ggcataagcg acatctgtca gtggacgata agcaccgtaa acggctctgc
     8041 gtcattccgg agtttatgag gcactaaggc gaacataaga gatggaatga gcatctaccc
     8101 gtttattatg ccacagagaa tcgggaaata acatccctta acacttgtta tgagataatt
     8161 ctgtaatcct ctttgcttcc tgagtgaata tttaacctga gcttgatcct acacatactt
     8221 attatgaatg ataaaattca ttcaattaat aacacatata ttaattgccg ttaaaactaa
     8281 aaaagcatta ataatcaacg caatataata aacctgcctt acatatcaac tgcgccagag
     8341 gtaggattga aaacgctctc ctgattttcc aattcatttt ctggataaat aaataattta
     8401 tttttgtcac tattatttat gtaatcatcc tgtcagggag agggatctca attatcaatg
     8461 cttaattacg tcatcatttt gatgacatga aaccggagca ttttgcatgg aattttcgtt
     8521 ttcacccaaa cttcttgttg ttgctgtcgc cgccgctctt cctctcatgg ccagcgcagc
     8581 agataccccg tcaactgcca ccgcacgcaa aggctttgcc ggatacgatc acccaaacca
     8641 gtatccacag aacgctgcaa tttattgaat ttgcacgatc atgtaggccg gataaggcgt
     8701 ttacgccgca tccggcaatc aaccgcaggc ggccacaaaa tggccgcttt tttacttatt
     8761 caccaccagc aaatgtgcct gcataatgtc actggccggg cgaccgtgcg ccagcaaatc
     8821 agccattgct ttaagatatg gcggtagatg gcggaaataa cgctgatacc cggcacacaa
     8881 ataattcagc cccggtttgc cgctggcatc gagcatgaag cggtgtttcg ggcagcctcc
     8941 ccagcacgct tttaacacgt tacaactgcg acactgcgcc ggtaattgct taaatttatc
     9001 ttcaccaaac acctgctgtt gcggggaatc gatcatttct gcaattgttt gctgatgcat
     9061 attccccagc cgatattgcg gataaacata gtgatcgcag gcgtaaacgt cgccgttgtg
     9121 ctcaacaatc accgagcgcc cacaggttgg ctgatgatgg caaaccgcac ccggcgcacc
     9181 gacaaaattg gcaaacgccc attcgatatt catcacgaaa atcttgccga cgtcgcgttt
     9241 gatccagtgg tcgaatatcg ccaccagaaa ttcaccaaat tcatcggggc gcaccgacca
     9301 ttccgtcagt tccccctgaa tatcaccagg cgcatgcaat ttcagtccat cgcgggcagc
     9361 tgtttcatca gccagacgct cgaccaccgg aataaactgg atgaattcga ctcccgcatc
     9421 gcacaaaaaa tcataaacct gcaacggttg ctgcgcgctg gtgcggttga cgcagaccag
     9481 cacgttatag tcgacatgat gtttttgcag cagcgtcagg gcacgcatca ccagcttatg
     9541 cgttggtctg ccacctttgg tcacgcgata ttgattgtgg atctcagccg gaccgtccag
     9601 cgataaccca acaagaaaat gattttctgc cagaaatgca caccatttat catcgagcag
     9661 cacgccgtta gtctggaagc tgttacttat cttcctgcca gcaccgtatt tcgcctgtaa
     9721 ctttacggca cggcggtaaa aatccagccc gagtaacgtt ggttcgccgc cctgccaggt
     9781 aaaagccact tcgttttgcg gttcgctggc agcgatatag tgacggacat acgcttccag
     9841 cgtgtcatcg tccatatgcg tgactggctt ttcgcggtaa agggattgtt tttcgagata
     9901 aaaacagtag tcacagttca gattacaatc ggaaccactc ggtttcgcca tcacatgaaa
     9961 agcacgcgtt ggaacctgtt gcagcatggt cactccttgc ctgaagtcat ccttcaatac
    10021 gttaagggcg aaatcatacc gtggggcaat gagttattac gggcgtttat tgcgaaggga
    10081 gggtgacgaa atgcacagaa aacaaaaaac cccgccgaag cggggttcaa aattggtcgg
    10141 cgagagagga ttcgaacctc cgacccactg gtcccaaacc agttgcgcta ccaagctgcg
    10201 ctactcgccg aaatactgct ttttgaattt ttagttcaat tctttaaagt cgtggtgcga
    10261 ggggggggac ttgaaccccc acgtccgtaa ggacactaac acctgaagct agcgcgtcta
    10321 ccaattccgc caccttcgca ttgtcacaac ttctaataat ggggtggcta atgggattcg
    10381 aacccacgac aactggaatc acaatccagg gctctaccaa ctgagctata gccaccacta
    10441 caaatcttgt tacgcggtat tactaccacc gcagctcaag cgccgggact aaatggcgcg
    10501 cccgacagga ttcgaacctg agacctctgc ctccggaggg cagcgctcta tccagctgag
    10561 ctacgggcgc ttagcgccgt tgcgggggtg gataatacgg acttcccacc cctctgtcta
    10621 gtcccttttt aaataaattg cgcgtttggt tacggtttgt gcgttttgct gcttattcct
    10681 ccagtttatg cgctttcccg tggcgattaa ggccaaaaac tttataaatc gccgtcaccg
    10741 ccagcataaa gatgatgcca acaaacagcg acatacgcgt atcttcatta aagtacatgc
    10801 cgatcaaaac gcaaatcagg aatgccattg ttacgtagtt ggcccacggg aacaggattg
    10861 agcggaacgg atggctggca atcgccgctt tatgtgcacg ccgaaaacgc agctggctta
    10921 tcaatatcac aaaccatggc accatccccg gaagcacgct ggcactgtag acgtagacaa
    10981 acacacgctg cggattggga atgatgtagt tcaggcatga gccaattagc agaatagcaa
    11041 tagatactgc cacacccgca actggtacgc cgtgacggga aactttcgcc atcgccgccg
    11101 gtaactgacg gtttttcgcc agtgcgtaga gcatacgtcc gcaactgtac atgccgctgt
    11161 tacagccaga gagcgcagcc gtcagcacca caaagttgat aatgcccgcc gctgcggtaa
    11221 taccgatttt ggcaaaagtc agtacgaacg ggctgccgtt gctgccaatt tcattccacg
    11281 ggaagatggt gacgataacg aaaatcgcgc ctacgtagaa aatcaggatc cgccacagca
    11341 ctttgcctac agcactgcgc agcgtcacct gcggattctt cgcttcaccg gcagtaatgc
    11401 caatcagctc cacgccctgg taggacgcca ccacaataca cagcgcggtc aggaaccctt
    11461 tccagccacc cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca atcgactgcc
    11521 cgccattgcc aaagccaaag aaaatcacgc ccaggccaat gacaatcatc acgataatcg
    11581 tggtgacttt gatcatcgcg aaccagaact cgatttcgcc gtacaaccgc accgccgcca
    11641 gattcgccaa cgccaccagc gccaccgcga tcaatgcagg tatccactgc gccatctccg
    11701 ggaaccagaa ctggacataa acgccaatgg cggtgatttc tgagatcccc accgccatcc
    11761 acataaacca gtaagaccag gcggtgagat aaccaaaaaa cgggctcata taacgatgcg
    11821 cataaacggc gaacgaaccg gtaacaggtt caaggaacaa catttcgccc attgaacgca
    11881 tgatgaaaaa gacgaacagc ccggcgatga tataggccaa caatacggat ggcccggccc
    11941 atttcagggt actggcggcc cccataaaca ggccgacgcc aatagtgccc cccagggcga
    12001 tgagttcgat atgtcgagct tccagcccac gctgtagctc tggtttgtta tctgccataa
    12061 atcctcgtgt tgtgtttgca tgctttccgg tgttaccggt tatcgttatg ggtacatcga
    12121 gtgttgcgaa tgttttcgta attcaggaga aatggcaaat aaagcattaa aaatttgaat
    12181 gctttgtgta ataaaaaagc agacaggcga cggagtgacc actccgtcgc tttacaaaga
    12241 gaggaaaatc agaggttgcc ggtgtagtgc cagcgtaaat aacgcagcaa acgaagctga
    12301 cgcttaatgc ggctcggctg cgaaagcagg cggtagagcc actccagtcc cagcgtttgc
    12361 cagattttcg gtgcgcgttt tacgtgaccg gtgaaaacat cgtaagtccc gccaacgccc
    12421 atatacagcg catctggatg caccagacgg cagtcgcgca tgaagatctc ctgctttggc
    12481 gatcccatcg caacggtgac gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc
    12541 tgacgctgct cgggtttaaa ataaccatcc tgactgccaa cgatattcac attccattgg
    12601 ttgcgcagtt tagcttcagt ttgcgccagc acttcaggtt taccgcccac aagaaatacc
    12661 ggcgtccctt ctttgcctgc gcgcgccatc agctcttccc agagatcggc accggcaacg
    12721 cgggaaacct gcgcctgcgg gtacttttta cgtactgaac gtacaacgct gatgccatcc
    12781 gcatatttaa attcggcagc gttaattaac tccctgacct cggcgttatc ttcaatggtc
    12841 agcatttttt cagcattaat ggcaaccagc gttccctgct taagctgccc gtcagcaaac
    12901 agataatcga gggcgtgctg catatcacgc caaccaatca actgtaagcc acgcagcgta
    12961 taggtcggtg ccgtggtgtt gttattcatt gttatccttc aacctgcgtc cggagcgatg
    13021 attttgtacg tttatgaatg agtccggcgc tttcaaaaag ccagtacagc agttttgcga
    13081 tcatcagaca tgcgccgaag accacgataa aaaagactac gcgtgagaca aacgaatcca
    13141 gcccttcacg cgccagcacg atcatattga agatcgcccc aaagcagaaa ctgtgcaata
    13201 tcgcagcctt atagcgatta ggctcgcggt tgcccagctc atacagccag tcgaaccatt
    13261 tgatgatcag accaaccacg atcgccccga gcgggatgaa caacgcgccg cccatcacca
    13321 ccagtgagcc tataagcgta ggcgagatcg ccagtccgga gtggttattc agcacttccc
    13381 aggtaaagta gttggctgag ttcagcacca tactcgggcg acccggccac agccaggaag
    13441 ggataaagac atagaaatcg cggacaattg gagccaggcc ctggaagtcg atgttgtcgt
    13501 agttctgcaa cagcaacgcc agattctccc acggggagaa ggtgtcgcga gtgagataga
    13561 gaaacgtata gaacgcttca tcgccgctca cattcattcc atagcgtttt agcgccatcc
    13621 agaacatgcc aacaatcccc agcacgcccg ccgccgccag catccacaac gaaatccagc
    13681 cgcgaataat gccaataaac aggaagatag cgaatgcgat gatgatattg gcgcgagtgc
    13741 cgccgacaat catataagtc agcaagccaa aggcgaccgt gctgacgagg aaaaacagcc
    13801 acgctttgct gtcctggcgc agaaagtaga ccaccagcat cgccgggatg aaaaagtaaa
    13861 agaagcgttt taacgccacg ccggagactt cactggagaa gatctgactg taggagttaa
    13921 gccggaacag caaaaagccg ttgtgcatga agaagatgcc gacgcttacc agcgcgatac
    13981 ccatcaggat cacccacgta agattggtct ccacgcggtt catggtaaac agcggacggc
    14041 gcggtacatc agcaacgcgt ttgcgtaggc gggttttgta ggtgacatag taaaccgcgt
    14101 agaagcagcc cgcagaaagc aacgcctgca acaagatttc tggcggcgcg acaccaacat
    14161 caaagcgaaa taccagcacg ctggtcagcg ggaagccgaa gaaaaaggtg agcaaaaaca
    14221 gcaatgaaaa gaagacattg aagttaaagc gcacacggcg aaactcaaac caggtcagcg
    14281 tggcaataaa cagcgtgcag agcagccaga ccacaaacag gccactgaat tgcagcagac
    14341 tcatgcgacc tccccggcgg caatcgccaa cgcccgctgc cagccttgta gatagttagg
    14401 gctaaagaag gcaatggtgt ttttatccac cgacgccaac tggcgctgcg cttcacgcac
    14461 aatatcctcg ttgagatcgt cggtagtaaa cagcaccggc aaatgctgtt ccgtcatatc
    14521 ctgccagaac ggattttccc ggttaagcac acaaggaatg cccgcctgaa tcagtaagca
    14581 aagcgtacca atgccctgct ggcgggcaaa aataaagtaa ccaagatcgc actgacgaag
    14641 tagcgccaga taggcgtcaa attccagttt ttcgctcaaa atttgcagat tttcttcgct
    14701 gaataactcc agccccgcct gacgaacttc ctcaatgtac gcttcgttat taggcggata
    14761 tcccatcggc accaccactt ttaccgtatc gccaaattgc tgatgaacgg cgcgcaaggc
    14821 agcaatatgg tcattgctgc ggtcaccgga gttccccacc agaatggtca ttttcccttc
    14881 acgttgccga tcattcgcca tcgtattgag agaagggtcc atccgcgtcg ggaagtacag
    14941 cagttcgccc cgcacctttg gatgcgtttt ggcaaaaaag ctcaaatcac cgcgggtggc
    15001 aaatacacag ccgactcgct tttgcgccag gcgacgtagc gggtaaaaaa gcttatatct
    15061 caagccactg gaaagctcat acaggtctgc cccccagata tgccagaaaa actggctggg
    15121 cttaatgcca ccactcagca gagccagcca cagtgtggga ttgaactgac cgtggaaaaa
    15181 aaatcgctgc tgacggttag cttttgcttt cgcgatgacc gcttccgcca gcgatttttt
    15241 cccagggaaa aattgcacag aaagcgccgg acagctatca cttaagccgt cgtccttgcc
    15301 aacaaccata aactcgcgcg cgtgctcgct cgtcgcggcc agcgcgtcat tgaaaaaccg
    15361 caaaacggtt cggttatggt gagggatatc cgatcccagt acgtgaatca gtacagtcat
    15421 gcccgcctac gccagagtaa aaacacgcca caacaaagag aaaaatagac gatataagtt
    15481 gccatatatg cctgcgccgc gcccagtgca ccatgcgcag ggattagcca gtgggcaaat
    15541 accatcaata aagtgaactg gctgacttcc gccagaatat aaaaccgcag tgacgctttg
    15601 gcgatcacca gataaccaaa gacataagcg cccactttta acacatcacc cactaactgc
    15661 caggcaaaga gatcgcgcat agcggtaaat ttattcgaca acagcaacca gatagcaaaa
    15721 tcacgcagca accagacggt aaaactcgcc gccgccactg ccggtaagac gaatttcagc
    15781 gatttaacca cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc
    15841 aaatatacgc tgaacgatgc cgtaataaat tgcaggtagg catcggaaat actgctcacc
    15901 ccttgccaga tccccacttc atcccagcta tactgcgccg ccagcagttt acgcatcatg
    15961 atgtaagcaa caggcaaggt caccgacgta atcaacgcca tgagcgtaaa tttgctcaac
    16021 tgccctgcca gaccgttatc ccagctgggt ttcagatagc ttaacgggat gacaccacgt
    16081 ttgatcaaca tgatggcggc aggaatcacc accagcgcgg gaatcagcgc cagacccagc
    16141 aacgcccctt cataaccgcc caaacggtaa ctgacgtagt acgcgagaac gccaatcaag
    16201 ctgccgacaa tcagcgataa cgcattacct gcggcatcgc gaaagccttt catcagcgcc
    16261 agtaacaggt tgccccaggc gatccccatt tgcaccagcg ccactaaacg caccagcccc
    16321 tgatagtcgg tattaccaaa cagtccctgg ctgattggcg cagctgccag cacaaaaacc
    16381 agcgccatca gcgtagagaa accaagtacc atcgctgatg aagtgccgac cacgcggcgc
    16441 agctgttgcg gattatcatg gtactgggca acgtatttgg ttacaccgtt aaagatgcca
    16501 gctccggcaa gcacgccgag cacggtaatc aactggcgga aatttgccgc cagcccaagc
    16561 cccgccggac caaatgacac agccagcaac ttaccgacca gtaacccggc accaatcttg
    16621 accagtgtac tggccgccgt ccacaaggac gcttttgcca acgacatatc aggagaagta
    16681 gttcaacaaa gtcgcaatta ccgtacgctg attgacgggc gacaggttgt agaacagcgg
    16741 caggcgcagc aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt ggaactcacc
    16801 aaagcgttcc cccgcagggc aaccgtgcag cggaatgtaa tggaacaccg ccatgatttc
    16861 cgcttctttc agaaagttaa tcaacgcgct ccggtcatca atatcccgca gtttaatgta
    16921 gaacatatgc gcgttctgca cgcagccatc gggaatcgac ggcagctcga tacgcccggc
    16981 tttcgccaga ggcgctaacg catcgtagta gttttgccac agcgccagac gttgctggtt
    17041 gatacgatcc gctgcttcca gttgcgccca caggtacgca gcttgcagat cggacatcaa
    17101 atagctggag ccaatatcgc gccaggtata tttatcgacc tgaccacgga agaactggct
    17161 gcggttagtg cccttttcac ggatgatctc ggctcgttcg attaacgctt tatcgttaat
    17221 cagcgtcgcg ccgccttcac cacccgccgt atagtttttg gtttcatgga agctaaagca
    17281 gccaatatga ccaatggttc ccagtgcacg ccctttgtaa gtggacatca cgccctgagc
    17341 ggcatcttct accacaaaca gattatgctt tttcgccaac gccataatgg tgtccatttc
    17401 gcaggccaca cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg tgatcgccgc
    17461 ttcaatcagc gtttcgtcga tgttcatggt gtccgggcga acatccacaa aaacgatttt
    17521 tgcgccacgc agcacaaagg cattggcggt ggagacaaag gtgtagctcg gcatgatcac
    17581 ttcatcgcca ggctggatat cgagcagcag cgccgccatc tccagcgaag cggtgcagga
    17641 cggcgtcagt aacactttgg cgctgccaaa acgttgctcc agccactgct ggcagcgacg
    17701 ggtaaaaccg ccatcgccac acagtttgcc gctacccatt gccgactgca tatagtcgag
    17761 ttcggttccc accaccggcg gtgcgttaaa tggaatcatg tgatcacctg tataaccagt
    17821 acgcggtgct ttctacattc gcaccacttt gtatgtatcg tttaagcgcg gcggtgttgc
    17881 ccatttgggt cgccacccgc aaagttgttt taccgcgagc atacgcccag tttagcgccg
    17941 tttgcatcag ctcagcacct gcaccgcgtc cagccagcag gccaattcgc gcatctgtcg
    18001 cattgagttc ccgtaaagag acataaccgc gaataccgcc ggacgccgca cgtaaaatca
    18061 gacattgatg atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca taaaagcgtc
    18121 cgctggcgtc aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat gcggcgctgg
    18181 ctaactgacg taatgcggga atatcggtct cttgtgccac tacagcacca ctatcactga
    18241 cattgttcac gggtagtgcc aaatcaactt caccttctac cagggagaat cccagctgtt
    18301 gcagggcatc cagttcaccc gtatttgatg ccgcaatttt ggcctgcacc cgtgaccacg
    18361 gcgctaacac gtctggcgtc aggagcggtg cttcagacgt aatgcgcacg atggcgctgt
    18421 taacaccaaa gaaggcgttt tcccaggtta gtggctcaat actggcgcgg acgggcacga
    18481 agtaactcca gcagatattg gccgtagcca gttttcgcta atgaactggc agcacgcttc
    18541 acaccctcgt catcgagcca gccgttacgc caggcgatct cttccaggca ggcaatctta
    18601 aagccctggc gtttttctac tgtctgcaca aaggtactgg cttcaatcag gctgtcgtga
    18661 gtgccagtgt ccagccaggc aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc
    18721 tcgaggtaca tctggttgat ggaggtaatc tccagttcac cacgctccga cggcttcacc
    18781 tgctttgcgt actccacgac tttactgtcg tagaaataaa gcccggtcac cgcccagttt
    18841 gacttcggct gttttggctt ttcttccagc gagatagcgc ggaaattgtc gtcaaactcc
    18901 accacgccaa agcgttccgg gtccatcacc tgatagccaa ataccgtcgc cccttccgtg
    18961 cgcgccgcaa catgacgcag cttcggactg aagccctgac cgaagaagat gttatcgccc
    19021 agcaccagac aagaaggttc accgttgagg aaggtttcac cgatgataaa ggcctgcgcc
    19081 agaccgtccg ggctgggctg ttcggcgtat tccagctgga taccgaactc actaccatcg
    19141 cccagcaggc gctggaaata acctttatct tccggcgtag tgatgatgag aatttcgcgg
    19201 atatcggcca acatcagcac cgacagcgga tagtaaatca ttggcttatc gtaaatcggc
    19261 aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc gggtgccgga accgcccgcc
    19321 aggataatac ctttcatgtt tgcctccgct gaaaattagc ctttcagacc taaacgctcg
    19381 ccctgatagc tgccgtcctg cacctgcttc caccagcttt cattagccag ataccactgc
    19441 accgttttac gcattccact ttcaaaggtt tcctgtggca gccagccaag ttcacgggca
    19501 attttcgaag catcaatggc ataacgcaga tcatgccccg gacggtcagc gacaaaggtg
    19561 atcaagtcac gatagtgcac cacgccgtgc ggcttgttcg gagccagttc ttccagcagc
    19621 tcgcaaatgg tttccacaac atcgagattc ttacgctcgt tgtgaccacc aatattatag
    19681 gtttcaccga ctttcccggt ggtcgccacg caatacagcg cgcgggcgtg atcttccaca
    19741 tacagccagt cacggatttg ctgcccgttg ccatataccg gcagcggttt acccgccagc
    19801 gcgttgagga tcatcagcgg gatcagtttt tccggaaagt ggtaaggacc gtagttattc
    19861 gagcagttgg tgataagcgt cggcagaccg taggtccgca gccaggcgcg caccagatgg
    19921 tcgctgctgg cttttgacgc ggaataaggg ctgctcggcg catacggcgt ggtttcggtg
    19981 aagaaatcat ccgtcgagtg caggtcacca tatacttcgt cggtggagat atgatgaaaa
    20041 cggaacgctg attttttatc ttccgtcagc gtattccagt aagcccgcgc cgcttcaagc
    20101 aatgtataag tcccgacaat gttggtttca ataaacgctg ccgggccgtc aatagaacgg
    20161 tcaacatggc tttcggctgc cagatgcatg acacagtctg gctgatgctc agtgaatacg
    20221 cgtgccagtt ctgcccgatc gcagatatca actttctcaa aggcaaagcg ctcgctttgc
    20281 gcgaccggtg ccagcgacat caggtttccg gcgtaggtca gcttatcgac cactaccacc
    20341 gcgtcgctcg tttcgttgat gatataacgc accagcgccg agccaataaa cccggcacca
    20401 cctgttatca gaatttttct catcagcgcc agactccttt ggcatcgacg acatactgct
    20461 gatggacatt gtcgccattg ataactttaa actgactatg atcgaccagc atcaccagca
    20521 cgtctgccgt tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg gtcagttttt
    20581 tcggcaactg gtggatgtta ggctcaacaa ccagagtttc gccgctatgc cactgggcga
    20641 tcagttcagc gatttccatc gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg
    20701 ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt atcggtagcc gccaggcaat
    20761 cagccaccgc cgctttcacc tgatcgataa cccagaacgg tttgtgatcg ttcacttcgc
    20821 gcgcggtacg gataagccgc gcctgctggg ggttctgtgc cacgataaac cacggatcaa
    20881 cagcaatgca gtgaccgccc acgccagggc caggctgaag aatattaacg cgagggtgac
    20941 gattcgccag gcgaatcagt tcccagacgt taatcccctg atcggcacaa atcagcgaca
    21001 attcattagc aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt
    21061 ccgccgtccg cgagttagtg acgacacact caccttcgag gaaaattttg tacagttcgc
    21121 tggcgcgggt cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt ttaatcagct
    21181 cgaccataac ctggcccggt aacacgcgtt ccgggcagta agcaatgttg acgtccgcct
    21241 gctcgcccac ctgctgtggg aaagtgagat ccggacgcat ctctgctaac cattctgcca
    21301 tcttctcggt tgaccccacc ggcgaggtgg attcaaggat caccagcgcg ccttttttca
    21361 gcactggcgc aatggagcga gcagccgatt caacgtaggt catatctggc tcatgatcgc
    21421 ctttaaacgg cgtgggtaca gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg
    21481 ctcgtaaaaa accgccttct acggcagttt ttactacgct cgccaaatca ggctcgacga
    21541 tatggatttc gccacgattg atggtatcaa ccgcgtgttg gttgatatcg acaccaatca
    21601 cctgtttttg ccgtgaggca aacgctgctg ctgtaggcag cccgatgtaa cccagtccga
    21661 taacagaaat ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg cttccagaat
    21721 gcgagagcat gcctgaccat caccatacgg gttatgggcg cggctcatag tttgatattc
    21781 gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac
    21841 cagacgcacc gtacccgccg tcaccgcttc cggacgctca gtggtatcgc gcatcaccag
    21901 caccggtttc cccagcgaag gcgcttcttc ctgaatgccg cctgagtcgg tcaaaatcag
    21961 ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc tggggatcga tcagaatgac
    22021 atttttcaca tgccccagaa tgcgattgac cggctctctg acgttcgggt tgagatgcac
    22081 cggatagaca atctggatgt cctggtgcgt ggtggcaatg tctgccaacg cgtggcagat
    22141 ttcttcaaag ccacgaccga aactttcacg cctgtgaccg gtcaccagaa tcatcttttt
    22201 atcggggtcg ataaacgggt aatttgccgc cagttctgaa cgcagcgtat cgctgctcat
    22261 cacctggtca cgcacccata acagtgcatc aataactgta ttaccagtaa tgaagattcg
    22321 gctatccgca acgttttcac gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg
    22381 atacatcgcc agatgcccgg tcaatgtacg gttagcctct tccggccacg gcgaataaag
    22441 atcgcccgtg cgcagaccag cctcaacgtg accaacagga atacgctgat aaaacgccgc
    22501 caggctggtt gccagcgtcg tcgttgtgtc gccgtgaacc agcacgacgt ctggtttgaa
    22561 ctcggcaaga ataggtttta gcccttccag aatccgacag gttatctctg tcaggccctg
    22621 tcctggctgc attatgttga gatcgtagtc aggtacaatg gaaaagagtt tcagcacctg
    22681 atcgagcatc tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa aaggatcttt
    22741 tgccaacgca tgcaccaacg gcgccatctt gatggcttcc gggcgcgtac caaatacagt
    22801 cagtactttc acatcgattc tcttcgaata agcggcgagc gcctttgcgc tcaccgcagc
    22861 agtgttgcta tttcgagcaa cggcgggtta atgcgacacc agccccgatc agccccccga
    22921 caatgcccca cataatcatc aggaaggcac gacgtgggct atcgcgtttt accggttctt
    22981 ccggcgtacg caaatagcga taggtctgaa aacgcggatc cagggttgga ccaacattca
    23041 gggtgtttaa catggcccga ttctgatcat agtcgagatc aaaggccgga ccgacggcct
    23101 gtaaattttc cagtcgagcc tggagcattg gacgcccaag caggaacatt tctgaatcag
    23161 gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt
    23221 tcagcgcctg ttcaatactg ttcatccggc ggtcgtagat ggctttcgcc acctcttcct
    23281 gacgcttcac ctgtgctttc atctggatgg tacgcgccgc ccatgcgcct ttcagctcat
    23341 cattcagatg gctggctgca cgctggctgg caaaagcaac atactgacgt aacaggttat
    23401 tagcgtcagg cgcggtttca gcaataagct tcacgctgtc attgaccgcg cgggtaaagt
    23461 ctccggggat aaactggatg ttgttaatca tttcatccag caacgccgca tcggctttgc
    23521 tgttgcccac catccgctgt ttgtaatagt cggtttgcag ccagaactct ctgcgggtat
    23581 cccacgaggc cagttgcata acaaactctt tataggcttc gtccatgacc gatggttggt
    23641 cggcagaagc catgtttgaa cggacatcca ggttacgcaa aaattgctgc tgcgagtaat
    23701 atccccccag catattcacc gttggacgat cggtaatcgc cgtcgagctc cactcctgac
    23761 gagcaaaaaa agtatacgcc agcgcgatta acgcaaatgc cagccccatg ccaataatcc
    23821 atagcttccc agcccacaag gtacgaaaca acccacgaat atccagttca ttttcagcgt
    23881 cttcggccgg tttcccaggc attggttgtg tcatcacatc ctcatttatt tggttaaatt
    23941 ggggctgcca ccacgatttc tacgcagtct gcgttttacg cgcttaataa agcgagcaac
    24001 tttccaggca cgcttaatgc aatatccata gaggaagaat gctagcaaaa agagcaccag
    24061 catgacccac tccgggacaa aatgagaata ttctgccagc acgccaatgg aagcgagcag
    24121 tgccgcagca agggtaatca gcacaaacgc ctgacgggaa gtaaacccgg cacgcatgat
    24181 caaatggtga atatgctgac ggtcaggaga gaatgggctc atgcctttac gcaggcgacg
    24241 gtacataatc gccaccatat ccattagcgg aatggcgatt atccacaaag cggtaaccgg
    24301 gctgatggga tgggttttgc cctgggtcgt ttcgagcagg atccagataa cggtaaaacc
    24361 aatcagcgta ctgcccgcat cacccataaa gactttgtag cggcgaccca ggataccaag
    24421 gttaagcatg atgtatggca ggatggcggc gatcatcgca aagcaccaga ttgcgaggct
    24481 ggtttgcccg tcgaaccaca aaatcatacc gattgctgca aacgagacgc aggacaaccc
    24541 gcccagcaag ccatcaatgc catcaaccat gttgaacgca ttaatggccg cccagacggc
    24601 aaatagcgtc aggaagtaac caaacggtcc gagcaccatc tcccaggagc caaagatata
    24661 acccaggcta ctgagataaa gtttgccgaa caccatcata acaatgccaa cagcggcctg
    24721 tatggtggca cggattttta cgctgatatc aaaacggtca tccagcgcgc caatgaaaac
    24781 aagcacaccg gcacaagcga gatagagaga tgcatgcgga atatagtaat cgacaattcc
    24841 gaacgtgaag caaatccctg cgtaaaccga aatcccccca acgagaggta tcaatccctg
    24901 gtgacgtttg cggaagtttg gtttatccac taaaccgact ttttttgcca ccttacgggc
    24961 aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg agatcagtac tcactgtcag
    25021 taaattcaca atgcatgctc tcagagaaaa ttattagcag aagtataacc acgaagacct
    25081 ttattcagaa gggaaagtcc gttccgaacc caccagtcct gtgcaaaaat taaccatttg
    25141 atatgcaaat tgcataacac gccagcaaat tccaggaatt ttcctaacat cgccagcgcg
    25201 gcattaagat tacagatata aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata
    25261 agacaaattt atgagcgttt catcatttcg aagaaatcgt cattggtctt ggtcatggcc
    25321 agtttattaa tgaggaattc cattgcatcg atttcgccca tcgggtgaat gattttgcgc
    25381 aggatccaca ttttctgcag ttcttcctga gtagtgagca gttcttcttt acgggtaccg
    25441 gaacggttgt agtcgatagc cgggaagacg cgtttttcag cgatcttacg agagaggtgc
    25501 agttccatgt tgcctgtacc tttaaactct tcgtagataa cttcgtccat tttagaaccg
    25561 gtatcgataa gcgccgtcgc gataatagtc aggctgccgc cctcttccac gttacgcgcc
    25621 gcaccaaaga agcgtttcgg acgatgcagg gcgttggcat ccacaccacc ggtcaacact
    25681 ttacctgacg ccggaacaac ggtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc
    25741 agaatgataa cgtctttctt gtgctcaacc aggcgtttgg ccttctcgat caccatttcc
    25801 gcaacctgaa cgtggcgaga tgcgggttcg tcaaaggtag aagcaacaac ttcacctttt
    25861 accagacgct gcatctcggt tacttcttcc ggacgttcgt cgatcagcag aaccatcagc
    25921 acacaatccg ggtggttgta agcaatgctc tgggcaatgt tctgcagcag catggtttta
    25981 ccggctttcg gcggtgccac aatcagacca cgctgaccac gaccgatagg tgatgccaga
    26041 tccagtacgc gagcagttaa atcttcagta gaaccgttac cacgttccat acgcagacga
    26101 gaatttgcgt gcagcggggt taagttctca aagaggattt tgttgcgggc gttttcaggt
    26161 ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat agcgttcacc ttctttcggc
    26221 gggcgaatct taccagagat ggtatcacca gtgcggaggt tgaaacggcg gatttggcta
    26281 ggggaaacgt agatgtcatc aggaccggcg aggtaggagc tgtctgcgga acggaggaaa
    26341 ccaaatccat cctgcaatat ctccagtacg ccatcaccaa agatatcttc gccactcttt
    26401 gcgtgctgct tcaggatggc aaaaataatg tcctgcttac gcatgcgagc caggttttcc
    26461 agccccatat tttcgccgag agtgatcagc tcagaaaccg gcgtattctt taattcggta
    26521 agattcatag tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat
    26581 ggtatggcag gatcatccat gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg
    26641 gtcacaggag agtacgcaga actgaaacga caagacggat tgagtgacga gccagaaatc
    26701 tgttcacttc gcatttaaga taaaacggga agcgttggaa attacaagat tcaaacttaa
    26761 taaggtatgt ttaatacgaa gtcaacacta agttagcatg actcacgccg ggcgtccagt
    26821 ttttagcgac ggggcacccg aacatgaaat tcccttacgc caggttagcg tcgaggaact
    26881 ctttcaactg acctttagac agtgcgccca ctttggttgc cgccacttca ccgtttttga
    26941 acagcagcag agtcgggata ccacggatgc catatttcgg cgcagtgcca gggttttgat
    27001 cgatgttcag ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt tcatccagaa
    27061 tcggggcgat cattttgcac ggaccgcacc actctgccca gaaatcgacg aggatcgccc
    27121 cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc
    27181 tcatatataa ctccacagga ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta
    27241 accaactaaa ggttgacttt atttcaccgg atacgctttc gtaaagcaat agtaagctga
    27301 tattctacca cactatgagc aaaacacatt taacagaaca gaagttttcc gacttcgccc
    27361 tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt tcataactgt acgcccattc
    27421 aggcactggc ccttccgctg acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg
    27481 gtaccgggaa aacgatggcg tttcttacgt caacgtttca ttatcttctc tctcatcctg
    27541 cgattgccga tcgcaaggtg aatcaaccgc gtgccttaat tatggcaccg acgcgtgaac
    27601 ttgccgtgca gatccatgcc gacgcagaac cgctggcgga agctactggc ctgaagctgg
    27661 gtctggctta cggtggtgat ggctacgaca aacagctgaa agtgctggaa agcggcgttg
    27721 acattctgat tggcaccacg ggtcgtttaa ttgactacgc caagcagaac cacattaacc
    27781 tcggtgccat tcaggtagtg gttctggatg aagccgatcg catgtacgat ctgggcttta
    27841 ttaaagatat ccgctggctg ttccgccgta tgccgcctgc aaaccagcgc ctcaacatgc
    27901 tgttctccgc cacgctttcg taccgggtac gtgaactggc gttcgagcag atgaacaatg
    27961 ccgaatatat tgaagtggaa ccggaacaga aaacgggcca ccgtattaaa gaagagcttt
    28021 tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc
    28081 cagacagagc gattattttc gccaatacca aacaccgttg tgaagagatc tggggccacc
    28141 tggcggcaga tggtcatcgt gtcggtttgt tgacaggcga tgtcgcgcag aaaaaacgtc
    28201 tgcgtattct tgatgaattt acccgtggcg atctggatat tctggttgcc accgacgttg
    28261 ccgcgcgtgg tttgcatatt ccggcagtga cgcacgtctt taactacgat ttacccgatg
    28321 actgtgaaga ttacgttcac cgtattggtc gtacaggtcg cgcaggcgca agcggtcact
    28381 ctatcagcct ggcgtgtgaa gagtatgcat tgaatttgcc tgctattgaa acctatattg
    28441 gtcactcaat tccggtaagc aaatacaatc cggacgcatt gatgaccgat ctgccaaaac
    28501 cgctgcgcct cacgcgccca cgtacaggca atggtccgcg tcgtactggc gctccgcgta
    28561 atcgtcgtcg ttcaggttaa ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga
    28621 tggcaagaga atgaatcccg atgagcatac tgtagtaagt gattcaggtg aaagaatgca
    28681 gccaacacag agacagattg aaggatgaag agtatgggtt ccacctcgtc gctgtatgca
    28741 gccattgatc tcggttcgaa tagttttcat atgctggttg tgcgcgaggt ggctggaagc
    28801 atccagacgc tgacgcgaat aaaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa
    28861 aatgccctgt ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa
    28921 cgtctgcaag atatccctcc ctcgcaaatt cgcgttgtcg ctacggcgac attacgtctt
    28981 gccgtcaatg cgggtgattt tattgccaat gcgcaggaaa tcctcggttg tccggtacag
    29041 gtgatcagcg gtgaagagga agcacgtctg atttatcagg gcgttgctca cacaactggc
    29101 ggtgccgatc agcgcctggt ggtggatata ggcggtgcca gtactgaact ggtaaccggc
    29161 acgggtgcac aaaccacctc gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa
    29221 cgctattttg ccgatcgtaa tctggggcag gaaaattttg atgctgcaga aaaagcggca
    29281 cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt
    29341 ggtgcttccg gcaccgtgca ggcgttacag gaaatcatga tggcacaggg gatggatgaa
    29401 cgcattaccc tggaaaagtt gcagcaattg aaacagcgag ccattcattg cggtcggctg
    29461 gaggaactgg agattgacgg gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg
    29521 gcgatcctga tcgccatttt taccgaactg aatattcagt gtatgaccct ggcgggcggt
    29581 gcgctgcgtg aaggcctggt ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc
    29641 agccgtacgc tgcgtaatat tcagcgccgc tttatgatcg atattgatca ggcgcagcgc
    29701 gtagccaaag ttgcggctaa cttcttcgat caggtggaaa aggaatggca tcttgaagca
    29761 ataagccgcg atttgctcat cagcgcctgt cagcttcatg aaatcggcct gagcgttgac
    29821 tttaaacaag cgccgcaaca cgctgcttat ctggtgcgta atctggatct tcccggtttt
    29881 acccccgcac agaaaaaatt gctggcgacg ctactgctca accagactaa tccggtcgat
    29941 ctctcatcgc tgcatcagca aaatgccgta ccgccgcgcg tcgcagaaca actctgccgt
    30001 ttactccgcc tggccatcat ttttgccagc cgtcgccgtg acgatctcgt gccagagatg
    30061 acattacagg ctaaccatga actgttgacc ttgacgcttc cgcaaggttg gctaacccaa
    30121 catccgctgg gtaaagagat tattgctcag gaaagccagt ggcagagcta tgtccactgg
    30181 ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg ctgacgcatc ttttccggcc
    30241 ttgattattt ccctcgtttt gccgccatca tcgctttcag attcgccaga tggctttgcc
    30301 ctttctgcat ccgttcttcg gcgctgacca ctttgcgctc ctgttcccaa atcagatcat
    30361 cctgcggcag ctccagcaaa aagcggctcg gctccgggcg caccagctcg ccgtactgac
    30421 ggcgttcttt acacagcgta aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg
    30481 ccagccgccg ctcctcatcg atattatctt catcgatgct gctctggtgc ggcaaaaacc
    30541 cttcttccat accgaccatg tagacataag gaaactccag cccttttgac gcgtgcagag
    30601 tcatcagttg cacctgatcc agctcttctt cactctcacc acgctccatc atgtcgcgca
    30661 aagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc atccagttcg ctgccttcca
    30721 gcatctccgt catccagcta aacagttggt tgacgttctt catgcgcatt tcggcggctt
    30781 tcgggctggg cgatgtttcg tacagccagg attcataatc catgccgtgg atcagatcgc
    30841 gcaccgcggc aatcggctcc cgctccgcca gacgctggat ttctgccaac cagtgagtaa
    30901 agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg actcaggccc atatcaaagc
    30961 tggcggtaaa catgctttta ttgcgcgtca tcgcccactc acccagcttt ttcagcgtcg
    31021 ccgggccaat ctctctcttc ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg
    31081 ggttagtcag cacgcgcaga taagccagca agtccttgat ttcaggacga gagaaaaacg
    31141 acgtaccacc agaaattttg tacgggatgc ggttttgcat caggaatttt tcaaacactc
    31201 gcgactgatg gttaccgcga taaagaatag cgtaatcttt gtactgcgtt ttattgacga
    31261 agtgatgcgc aatcagttca ccagtgacgc gctcggcttc atgttcttcg ttattcgcgc
    31321 ttaaaacttt aagctctgtg ccgtaaccca gttcggagaa cagacgcttc tcaaagacgt
    31381 gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa gagcggtagt
    31441 tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg actcagcagc accaggtttt
    31501 gtggacgtgc gccgcgccag gagtagatcg actggtcgtc atcgcccacc acggtaaagc
    31561 gcgcgcggct gcccaccagc agtttcacca gttcatactg gctggtgttg gtgtcctgat
    31621 actcatccac cagcagatag cgaattttgt tctgccagcg ctcgcggact tcttcattgc
    31681 gttgcagcag caacgtcggc aataaaatca gatcatcgaa gtcgagaaca ttgcacgctt
    31741 tcaggtgtgc atcatacagc ccataacaat gggcaaaaat acggtcccgc tcgccaatcg
    31801 cactggctgc cgcctgggac ggtgttttga gatcgttctt ccagttagag atggtcgaaa
    31861 tcaactgttg caggagaact ttgtcatctt caatcagccc ttcggtcaac tctttgagca
    31921 acgccagctg atcggtatcg tcaaacagcg aaaagttcgc cttcatcccc agcgccgcat
    31981 actcacgctt gatgatatcc agccccagcg tgtggaaggt tgagatcatc agcccacgcg
    32041 cctctttgcg acccagcgtc tgtccgacac gctctttcat ctcgcgcgcc gccttattgg
    32101 taaaggtcac cgccgcgata tgtcgcgcct gataaccgca accgcggatc agatgggcga
    32161 ttttattggt gatcacacga gttttaccgg aacccgcgcc cgccagcacc aggcaggggc
    32221 cggtaacgaa ttcgacagct tgttgttggc cggggtttag acgcataggt gtattgctca
    32281 atcttcgaac ggggggagga ttgtagcaga atcgcatttg aatgtgaccg cgtgtaagcc
    32341 gctaaccgat tggctgcgga tgtttaaatc agagagggaa gaccgaaacc ggagttcatc
    32401 gtgaatccat tagacttaga aaatatcggg tcgtaacgtt gtgttattac tttttcagag
    32461 tttactaagg agaggtgtac tcgtggcgat taatttcagt cctaaggttg gtgaaatact
    32521 ggattgcaac tttggtaact atcctgttag ccagaatgga cagttcagca ccacgtatta
    32581 cgatggccgc attcctcctg aaatgataaa aaacaggtta gt
//
LOCUS       DAQSWQ010000044        30162 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.044, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000044 DAQSWQ010000000
VERSION     DAQSWQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.044"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="R8G18_002088"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="R8G18_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEE9795781.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="R8G18_002089"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="R8G18_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEE9795782.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="R8G18_002090"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="R8G18_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEE9795783.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="R8G18_002091"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="R8G18_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEE9795784.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="R8G18_002092"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="R8G18_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEE9795785.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="R8G18_002093"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="R8G18_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEE9795786.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="R8G18_002094"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="R8G18_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEE9795787.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="R8G18_002095"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="R8G18_002095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEE9795788.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="R8G18_002096"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="R8G18_002096"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEE9795789.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="R8G18_002097"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="R8G18_002097"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HEE9795790.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="R8G18_002098"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="R8G18_002098"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEE9795791.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="R8G18_002099"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="R8G18_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEE9795792.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="R8G18_002100"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="R8G18_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HEE9795793.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="R8G18_002101"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="R8G18_002101"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEE9795794.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="R8G18_002102"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="R8G18_002102"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEE9795795.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="R8G18_002103"
     CDS             complement(18516..18992)
                     /locus_tag="R8G18_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HEE9795796.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="R8G18_002104"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="R8G18_002104"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEE9795797.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="R8G18_002105"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="R8G18_002105"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEE9795798.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="R8G18_002106"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="R8G18_002106"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HEE9795799.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="R8G18_002107"
     CDS             21688..22278
                     /locus_tag="R8G18_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HEE9795800.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="R8G18_002108"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="R8G18_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HEE9795801.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="R8G18_002109"
     CDS             22750..23445
                     /locus_tag="R8G18_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HEE9795802.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="R8G18_002110"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="R8G18_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HEE9795803.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="R8G18_002111"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="R8G18_002111"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HEE9795804.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="R8G18_002112"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="R8G18_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HEE9795805.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="R8G18_002113"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="R8G18_002113"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEE9795806.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="R8G18_002114"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="R8G18_002114"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEE9795807.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7057 a         7872 c         8310 g         6923 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaagcgcgtc gc
//
LOCUS       DAQSWQ010000045        29569 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.045, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000045 DAQSWQ010000000
VERSION     DAQSWQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29569
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.045"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="R8G18_002115"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="R8G18_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEE9795808.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="R8G18_002116"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="R8G18_002116"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HEE9795809.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="R8G18_002117"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="R8G18_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HEE9795810.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="R8G18_002118"
     CDS             complement(3644..4030)
                     /locus_tag="R8G18_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HEE9795811.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="R8G18_002119"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="R8G18_002119"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HEE9795812.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="R8G18_002120"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="R8G18_002120"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEE9795813.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8607)
                     /gene="glnD"
                     /locus_tag="R8G18_002121"
     CDS             complement(5914..8607)
                     /gene="glnD"
                     /locus_tag="R8G18_002121"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HEE9795814.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNL
                     SVHDAQIFTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQ
                     PAKLRHFTVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITT
                     IGERVEDLFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8669..9463)
                     /gene="map"
                     /locus_tag="R8G18_002122"
     CDS             complement(8669..9463)
                     /gene="map"
                     /locus_tag="R8G18_002122"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEE9795815.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9831..10556
                     /gene="rpsB"
                     /locus_tag="R8G18_002123"
     CDS             9831..10556
                     /gene="rpsB"
                     /locus_tag="R8G18_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEE9795816.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10814..11665
                     /gene="tsf"
                     /locus_tag="R8G18_002124"
     CDS             10814..11665
                     /gene="tsf"
                     /locus_tag="R8G18_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEE9795817.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11812..12537
                     /gene="pyrH"
                     /locus_tag="R8G18_002125"
     CDS             11812..12537
                     /gene="pyrH"
                     /locus_tag="R8G18_002125"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEE9795818.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12829..13386
                     /gene="frr"
                     /locus_tag="R8G18_002126"
     CDS             12829..13386
                     /gene="frr"
                     /locus_tag="R8G18_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEE9795819.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13478..14674
                     /gene="ispC"
                     /locus_tag="R8G18_002127"
     CDS             13478..14674
                     /gene="ispC"
                     /locus_tag="R8G18_002127"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEE9795820.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14863..15621
                     /gene="ispU"
                     /locus_tag="R8G18_002128"
                     /gene_synonym="uppS"
     CDS             14863..15621
                     /gene="ispU"
                     /locus_tag="R8G18_002128"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HEE9795821.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15634..16491
                     /gene="cdsA"
                     /locus_tag="R8G18_002129"
     CDS             15634..16491
                     /gene="cdsA"
                     /locus_tag="R8G18_002129"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEE9795822.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16503..17855
                     /gene="rseP"
                     /locus_tag="R8G18_002130"
     CDS             16503..17855
                     /gene="rseP"
                     /locus_tag="R8G18_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HEE9795823.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17885..20317
                     /gene="bamA"
                     /locus_tag="R8G18_002131"
     CDS             17885..20317
                     /gene="bamA"
                     /locus_tag="R8G18_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEE9795824.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20439..20924
                     /gene="skp"
                     /locus_tag="R8G18_002132"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20439..20924
                     /gene="skp"
                     /locus_tag="R8G18_002132"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HEE9795825.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20928..21953
                     /gene="lpxD"
                     /locus_tag="R8G18_002133"
     CDS             20928..21953
                     /gene="lpxD"
                     /locus_tag="R8G18_002133"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEE9795826.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22058..22513
                     /gene="fabZ"
                     /locus_tag="R8G18_002134"
     CDS             22058..22513
                     /gene="fabZ"
                     /locus_tag="R8G18_002134"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEE9795827.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22517..23305
                     /gene="lpxA"
                     /locus_tag="R8G18_002135"
     CDS             22517..23305
                     /gene="lpxA"
                     /locus_tag="R8G18_002135"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEE9795828.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23305..24453
                     /gene="lpxB"
                     /locus_tag="R8G18_002136"
     CDS             23305..24453
                     /gene="lpxB"
                     /locus_tag="R8G18_002136"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEE9795829.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24450..25046
                     /gene="rnhB"
                     /locus_tag="R8G18_002137"
     CDS             24450..25046
                     /gene="rnhB"
                     /locus_tag="R8G18_002137"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEE9795830.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25083..28565
                     /gene="dnaE"
                     /locus_tag="R8G18_002138"
     CDS             25083..28565
                     /gene="dnaE"
                     /locus_tag="R8G18_002138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEE9795831.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28578..29537
                     /gene="accA"
                     /locus_tag="R8G18_002139"
     CDS             28578..29537
                     /gene="accA"
                     /locus_tag="R8G18_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEE9795832.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7164 a         7124 c         8164 g         7117 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtagtttgc ttaaatcatg
     6541 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
     6601 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
     6661 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
     6721 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
     6781 ccagctattc cacagcgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
     6841 atagtgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
     6901 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
     6961 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
     7021 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
     7081 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
     7141 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
     7201 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
     7261 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagcccgc gccgcaccgc
     7321 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tccggaatat tacacagcgg
     7381 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
     7441 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
     7501 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgat
     7561 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
     7621 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
     7681 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
     7741 gaataacagg cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
     7801 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
     7861 aaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
     7921 gatatcgcgc aagccgccag ggctactttt gatgtctggt tcaagattgt agctggtgcc
     7981 gtggtaacgc tgatggcgct ggttctgttc ttcaactttc gccgcgtaga acttgtcgga
     8041 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
     8101 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccag
     8161 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
     8221 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
     8281 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcgac
     8341 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
     8401 gtcgataaac tcggtgcgcg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
     8461 atcacccagc caacgctgga aagtatcgat atgggctttt atcccaccga cggttaattc
     8521 atcacggggc cagacgcatg gattttgcgg ttgaccgggc agggtgggga gagcggtgtt
     8581 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
     8641 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
     8701 tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc agtcaccaca atagtatgct
     8761 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
     8821 ggatctcttt tttacccgcg ttgaccattg gctcgatggt gaacgtcatc ccaggtttca
     8881 gtacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
     8941 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaact
     9001 tctgaatcgc cgcaccgatt tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca
     9061 ggtacaggct ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc ggcttaccga
     9121 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
     9181 taacgatatc gccatctttc agcagcttcg catcgtccgg gataccgtgg cacaccactt
     9241 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
     9301 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
     9361 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
     9421 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
     9481 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
     9541 gagaatcatt ctgaatttcg ccaaacgtgc cactgaacgt tttctataat agaaaattca
     9601 acgtctgatg ctgtacacag cgccaacaat tattggtgtc catgacgtat ttgtggtata
     9661 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
     9721 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
     9781 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
     9841 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
     9901 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
     9961 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gcttctcgca
    10021 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    10081 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    10141 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    10201 gtactttcga aaagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    10261 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    10321 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacctgggt attccggtat
    10381 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    10441 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    10501 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    10561 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    10621 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    10681 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    10741 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    10801 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    10861 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    10921 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    10981 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    11041 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    11101 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    11161 agaagaacgt gttgcgctgg tagcgaaaat tggtgagaac atcaacattc gccgcgttgc
    11221 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    11281 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    11341 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    11401 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    11461 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    11521 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    11581 catccgcttc gaagtggggg aaggcatcga gaaagttgag actgactttg cagcagaagt
    11641 tgctgcgatg tccaagcagt cttaattatc gaaaaggagc cgcctgaggg cggcttcttt
    11701 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    11761 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    11821 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    11881 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    11941 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    12001 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    12061 accgtaatga acggcctggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    12121 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    12181 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    12241 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    12301 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    12361 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    12421 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    12481 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    12541 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    12601 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    12661 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    12721 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    12781 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    12841 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    12901 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    12961 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    13021 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcg
    13081 tccgatcttg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    13141 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    13201 cgtgttgcag tacgtaacgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    13261 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    13321 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    13381 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    13441 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    13501 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    13561 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    13621 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    13681 gctacagcaa cagggtagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    13741 ggcagcgctt gaggatgttg atcaggtgat ggcagccatt gttggcgctg ctgggctgtt
    13801 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    13861 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    13921 ggtcgatagc gaacataacg ccatttttca gagtttaccg caacctatcc agcataatct
    13981 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    14041 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcctgccg
    14101 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    14161 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    14221 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    14281 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    14341 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    14401 cgcaccggat tatgatcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    14461 tcaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    14521 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tctgtactgg aaaaaatgga
    14581 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt
    14641 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    14701 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    14761 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    14821 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    14881 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    14941 aatggtcgct gggcaaaaaa gcaagggagg attcgtgcct ttgggcataa agccggggca
    15001 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    15061 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    15121 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    15181 cgtattattg gcgataccag tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct
    15241 gaagcgctaa cagctgggaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    15301 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    15361 caaccagatc agatagatga agagatgcta aaccagcatg tctgtatgca tgaactggcc
    15421 cctgtagatt tagtaattag gactgggggg gagcatcgca ttagtaactt tttgctttgg
    15481 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    15541 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    15601 cccggtgatg aaacagcctg atggggggcg cttttgctga agtatcgcct gatatctgct
    15661 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    15721 atcgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    15781 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    15841 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    15901 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    15961 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    16021 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    16081 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    16141 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    16201 tggcaaggct ttataggtgg actagcgacc gcagcggtaa tctcatgggg ttatggcatg
    16261 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    16321 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    16381 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    16441 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    16501 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    16561 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    16621 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    16681 ttatggccct gattccgctg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    16741 tcgttccgga actccgccac catgccttca ataataaatc tgttggccaa cgagcggcga
    16801 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    16861 ttattattgg tgtgcctggc gtacgtccgg tggttggtga aatagcagcc aattcgatag
    16921 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    16981 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    17041 ttacagtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    17101 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    17161 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    17221 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    17281 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    17341 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    17401 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    17461 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    17521 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    17581 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    17641 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    17701 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    17761 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    17821 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    17881 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    17941 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    18001 tggtgcggca ctcctcagta tgccggtgcg cacaggcgac acggttaatg atgaagatat
    18061 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    18121 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    18181 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    18241 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    18301 cttctactac agcgtcggta aatatagcgc cagcgtaaaa gctgtcgtga ccccgctgcc
    18361 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    18421 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    18481 gcgtgacgaa gtgccgtggt ggaacgtggt aggcgatcgt aaataccaga aacagaaact
    18541 ggcgggcgac cttgaaaccc tgcgcagcta ctatctggat cgcggttatg cccgtttcaa
    18601 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    18661 cattaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    18721 gcactccgct gaaattgagc agctgactaa gatcgagccg ggcgagctgt ataacggcac
    18781 caaagtgacc aagatggaag atgacatcaa aaagcttctc ggtcgctatg gttatgccta
    18841 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    18901 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    18961 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggctgggaag
    19021 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    19081 taccgatacc caacgtgttc cgggtagccc ggaccaggta gatgtagtct acaaggtgaa
    19141 agaacgtaac accggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    19201 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    19261 cgggaccaaa aacgattacc agacctatgc tgaattgtcg gtaaccaacc cgtacttcac
    19321 tgtagatggt gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    19381 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    19441 caacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    19501 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    19561 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    19621 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    19681 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    19741 gccgatcgat gacgatcaca aatgggttgt tctggggcgt acccgctggg gttatggtga
    19801 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    19861 cgtgcgtggc ttccagtcca atacaattgg tccgaaagca gtttacttcc cgcatcaggc
    19921 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    19981 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    20041 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt ccttcttctg
    20101 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    20161 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    20221 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    20281 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    20341 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    20401 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagcgg
    20461 caggtctcgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    20521 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    20581 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    20641 tgaaaaagct gcagtccatg aaagcgggca gcgatcgcac taagctggaa aaagacgtga
    20701 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    20761 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    20821 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    20881 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    20941 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    21001 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    21061 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    21121 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    21181 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    21241 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    21301 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    21361 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    21421 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    21481 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    21541 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    21601 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    21661 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    21721 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    21781 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    21841 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    21901 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    21961 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    22021 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    22081 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    22141 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    22201 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    22261 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    22321 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    22381 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    22441 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    22501 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    22561 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    22621 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    22681 aattggccgc gataatgaga tttatcagtt cgcctccatc ggcgaagtta accaggatct
    22741 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    22801 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    22861 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    22921 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    22981 catgaccgca gtccatcagt tctgcatcat tggtgcgcac gtgatggttg gcggctgctc
    23041 cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg caacgccgtt
    23101 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    23161 ccgcaatgcg tataagctga tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    23221 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    23281 ctcaacgcgc ggtctgattc gttaatgact gaacagcgtc cattaacgat tgccctggtc
    23341 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacat
    23401 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    23461 gcttggtacg aaatggaaga actggcggta atgggcattg ttgaagtgct cggtcgtctg
    23521 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    23581 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    23641 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    23701 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    23761 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    23821 ccgttagatc cagataaaaa tgccgcccgt gatgtgctgg ggatccctca cgatgcccac
    23881 tgcctggcgt tgctaccggg gagccgtggt gcagaagttg aaatgcttag tgccgatttc
    23941 ctgaaaacgg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccactg
    24001 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctt
    24061 tcagttcatt tgctggatgg gatgggccgt gaggcgatgg tcgccagcga tgcggcgcta
    24121 ctggcgtcgg gtacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    24181 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    24241 tcgctgccaa atctgctggc gggcagagag ttagttaaag aattattgca ggaagagtgt
    24301 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    24361 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    24421 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    24481 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    24541 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    24601 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    24661 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    24721 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    24781 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg
    24841 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    24901 atattgtttt cccgcaatat ggttttgccc aacacaaagg gtacccaacc gcttttcatc
    24961 tggaaaaact ggctgaacac ggcgcgaccg aacaccatcg gcgcagcttt gggcctgtca
    25021 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    25081 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    25141 gcctggccaa aaccgcaccg ttggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    25201 cgatcaccga tttcaccaac ctttgtggtc tggtgaagtt ctacggagcg ggacatggcg
    25261 cagggattaa gcctatcgtc ggggcagatt ttaacgtcca gtgcgacctg ctgggtgatg
    25321 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctgacgttgc
    25381 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    25441 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cggacgcatg ggcgacgtcg
    25501 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    25561 aacacttccc ggatcgctat tttcttgagc tgatccgcac cggcaggccg gacgaagaaa
    25621 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacca
    25681 acgacgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    25741 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    25801 tgcgtagcga agaggagatg tgcgagctgt ttgccgacat ccccgaagct cttgccaaca
    25861 ccgttgagat cgccaaacgt tgtaacgtaa ccgtgcgtct tggtgaatac ttcctgccgc
    25921 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagagggcc
    25981 tggaagagcg tctggccttt ttattccctg acgaggaaga acgtgttaag cgccgcccgg
    26041 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    26101 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    26161 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    26221 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    26281 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgag cacgtagcgg
    26341 acatgtacgg tcgtgatgcg gtatcgcaga tcatcacctt cggtacaatg gcggcgaaag
    26401 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gatcgtatct
    26461 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    26521 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    26581 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    26641 ccaaaattac cgattttgcg ccgctttact gcgatgaaga gggcaaacat ccggtcaccc
    26701 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    26761 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    26821 gcgagccgcc gctggatatc gctgcgatcc cgctggatga taagaaaagc ttcgacatgc
    26881 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    26941 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    27001 ccggcccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgcgaag
    27061 agatttccta tccggacgta cagtggcagc atgaaagcct gaaaccggta ctggagccaa
    27121 cctacggcat catcctgtat caggaacagg tcatgcagat tgcgcaggtg ctttctggtt
    27181 ataccctcgg tggcgcggat atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga
    27241 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    27301 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    27361 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    27421 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    27481 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    27541 tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc gccatcaaag
    27601 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    27661 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    27721 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    27781 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    27841 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    27901 gctgccaacc gtggccggag caggtggtgt tagatgggga acgtgaaacg ttaggtctgt
    27961 accttacggg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    28021 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    28081 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    28141 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    28201 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    28261 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    28321 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    28381 gactccgtca gtctctggaa gcccaccgct ctgggacaat tccagtacat ctctactatc
    28441 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    28501 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    28561 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    28621 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    28681 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgaca cgtaaaatct
    28741 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccacag cgtccttata
    28801 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgcgcgt
    28861 atgcagacga taaagctatc gtcggtggta tcgcccgtct tgacggtcgt ccggtgatga
    28921 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    28981 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    29041 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    29101 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctc ggcgtaccgg
    29161 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    29221 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    29281 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    29341 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    29401 ctcaccgtaa cccggaagcg atggcggtat cgttgaaagc gcaactgctg gcggatctgg
    29461 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    29521 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcgg
//
LOCUS       DAQSWQ010000046        28894 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.046, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000046 DAQSWQ010000000
VERSION     DAQSWQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.046"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1262)
                     /locus_tag="R8G18_002140"
     CDS             complement(<1..1262)
                     /locus_tag="R8G18_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HEE9795833.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
     gene            1753..2310
                     /gene="pinE"
                     /locus_tag="R8G18_002141"
                     /pseudo
     CDS             1753..2310
                     /gene="pinE"
                     /locus_tag="R8G18_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2649..3497)
                     /locus_tag="R8G18_002142"
     CDS             complement(2649..3497)
                     /locus_tag="R8G18_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEE9795834.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3706..4341
                     /gene="yfhb"
                     /locus_tag="R8G18_002143"
     CDS             3706..4341
                     /gene="yfhb"
                     /locus_tag="R8G18_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HEE9795835.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4366..4902
                     /gene="tadA"
                     /locus_tag="R8G18_002144"
     CDS             4366..4902
                     /gene="tadA"
                     /locus_tag="R8G18_002144"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEE9795836.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="R8G18_002145"
     CDS             complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="R8G18_002145"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HEE9795837.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6713..10600
                     /gene="purL"
                     /locus_tag="R8G18_002146"
                     /gene_synonym="purI"
     CDS             6713..10600
                     /gene="purL"
                     /locus_tag="R8G18_002146"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEE9795838.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11158..12585
                     /gene="qseE"
                     /locus_tag="R8G18_002147"
     CDS             11158..12585
                     /gene="qseE"
                     /locus_tag="R8G18_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HEE9795839.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12750..13463
                     /gene="qseG"
                     /locus_tag="R8G18_002148"
     CDS             12750..13463
                     /gene="qseG"
                     /locus_tag="R8G18_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HEE9795840.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13453..14787
                     /gene="glrR"
                     /locus_tag="R8G18_002149"
     CDS             13453..14787
                     /gene="glrR"
                     /locus_tag="R8G18_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HEE9795841.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14848..15186
                     /gene="glnB"
                     /locus_tag="R8G18_002150"
     CDS             14848..15186
                     /gene="glnB"
                     /locus_tag="R8G18_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HEE9795842.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="R8G18_002151"
     CDS             complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="R8G18_002151"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HEE9795843.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16749..18002
                     /gene="glyA"
                     /locus_tag="R8G18_002152"
     CDS             16749..18002
                     /gene="glyA"
                     /locus_tag="R8G18_002152"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEE9795844.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(18200..19393)
                     /locus_tag="R8G18_002153"
                     /pseudo
     CDS             complement(18200..19393)
                     /locus_tag="R8G18_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19511..22788
                     /locus_tag="R8G18_002154"
                     /pseudo
     CDS             19511..22788
                     /locus_tag="R8G18_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22885..23868
                     /locus_tag="R8G18_002155"
     CDS             22885..23868
                     /locus_tag="R8G18_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEE9795845.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23891..25402
                     /locus_tag="R8G18_002156"
     CDS             23891..25402
                     /locus_tag="R8G18_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEE9795846.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25427..26425
                     /locus_tag="R8G18_002157"
     CDS             25427..26425
                     /locus_tag="R8G18_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9795847.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26458..27552
                     /locus_tag="R8G18_002158"
     CDS             26458..27552
                     /locus_tag="R8G18_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEE9795848.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27564..28436
                     /locus_tag="R8G18_002159"
     CDS             27564..28436
                     /locus_tag="R8G18_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEE9795849.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28484..>28894)
                     /locus_tag="R8G18_002160"
     CDS             complement(28484..>28894)
                     /locus_tag="R8G18_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEE9795850.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6866 a         7144 c         7865 g         7019 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagctct cagtggcatc agcagcaaca gtgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggagaatg cccgggaatt ctcctcggtg
      241 gaaaaagaag tccattgtgt agaaacctcc cgacgatctg cttcaggaaa ccaggctgcc
      301 acagtttcgg gaagaggggg gatctgaaag ttctgccccc cttcagtgat gactctgata
      361 tggcgcgaat gtaaattttg cggcaacatc tggcgtaaaa acagtctgtt ttggtctgac
      421 agggaaatac gaaactgagg cccgttaaaa tttggaaccg aagtttgctg tattaagaat
      481 agcagagttt gatctgataa ggggttattt gcaatctcaa tagttatatg aggatctaaa
      541 cgaaaaagat ttgtcgggat tgtagttatt tggttgtttt caaaataaaa aaatcttctc
      601 atgataatat tttcttggat gtttggtaag ttgcttaatc tattgctata tgcaaaaaga
      661 agttgcagtc tcctaggtaa agggggcagt tcagtaagat ggttgtgcgc aaccttaagt
      721 atcttaagat tagaaggcag agatggaaga gtttgcagtt cgttgttcct aacctcaagt
      781 ttttttaaat tcaagggtaa tgaaggcagt gtggataacc tattatactc tgcgcttagt
      841 gatttgatgg tgtctggtaa taacggcaga ttctctaatt gattttcgga cacatccaaa
      901 gttattagat aatgtggtaa tgcagatata tgtgttaaga aattatctct tgcaataagt
      961 tctttaatat tttcaggtaa atcagtaagg atggttaatt tattacagct cacatcaagc
     1021 tttttaatgc aagcagggag tacaggtaac gaggaaagcg ccaaggctcg caggtccagt
     1081 ttatgcccct tagaggtaag acatgcacga agtcttctta ccgcctcagt acgctgttct
     1141 tccggtaacc cctgtttctc ccaatcatcc cattcggaca aataatggtt gggggatgta
     1201 attgtgctga tatgtggaaa taaacaggat gaaatatttc tgagcatatt aatttctcgc
     1261 atatggatat ggaattagat tgtgaataaa ttgtatttaa tgtatctctc ctaatgagga
     1321 gggtgattca gaatattcgc cataagggaa tgagtgttat atcatgcttt ctaccctggt
     1381 aagtatattt gcttttttcg tatatttggt tcatcttttt ttgatgtttt acatattcac
     1441 aatgcagagg atttatacca gaagacatta gtcttgagta agatactaca acatctccaa
     1501 ctattttagc cagcctgact acttgacttg gtaatatttg ttgctaataa tcatgtataa
     1561 aataagctta tatacatagt aacttcagca aaggatttag taatgatcca aatcatatct
     1621 caatgtactg tttttttctt taaaaaaaca gtacattcta ctttataatg actgtgtatt
     1681 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
     1741 gggggattta ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
     1801 ctacaacgta atgcgttgaa ctgtgcagga tgcgagctga tttttgaaga caagataagt
     1861 ggcacaaagt ccgaaaggcc ggggctgaaa aaactgctca ggacattatc ggcaggcgac
     1921 acactggttg tctggaagct ggatcggctg gggcgcagta tgcggcatct tgtcgtgctg
     1981 gtggaggagt tgcgcgaacg aggcatcaac tttcgtagtc tgactgattc aattgatacc
     2041 agcacaccaa tgggacgctt tttcttccat gtgatgggtg ccctggctga aatggaacgt
     2101 gaactgattg ttgaacgaac aaaagcagga ctggaagctg ctcgcgcaca gggacgaatt
     2161 ggcggtcgcc gtcccaaact ttcaccagaa taatgggcgc aggccgggcg actaattgca
     2221 tcaggagttt ctcgccagaa ggtggcgatc atctatgatg ttggcgaatc gacttttttg
     2281 tataagaggt ttccagccag ggttatataa aaccgcagac acggcgtata caagaacgtg
     2341 ctacggttga ctgaaaagtt aatgtggtat gtcacaggcc ttttgcgccg taaagccccg
     2401 tcctttaggg cgcggaggac gttaaagaaa gtggtgttct ttgtgttgct gaacttatgc
     2461 aaaatttttt tgcaccataa atccaacaac acattcaata acacattgct gtatcactat
     2521 gaaaataaat gtgtttttat ttgatttcac acatatcaca attgatgcat tttttagtga
     2581 ttaacaacgc cataaggaaa ctctcaaaac aacacaaacc gggcgggcat tgtacgcgca
     2641 ttctttactc agaccagttt tttcaccagc gcttcactat gacgaatacg ttctggtgcc
     2701 agttccagat cctgctgaat cagcgcaatg aacagcaaat ccgttaacat tccctgagcg
     2761 tgacaagcag agattgaagc actgtttgtc gcttgttctt cggcaatggt atacaggcaa
     2821 tgagaagcac gctgttgcag ggcattcgga gtaaagccgg taatcgccag cacttttccg
     2881 cccactcgca gcatctcatc tgccgccagg tttaactcgc gtcgtacacc ggtgtaggaa
     2941 atggctaata acagatcgtc aggggacgac gcctgtactg ttgcgagtag cgcatgcata
     3001 tcgcgcactg cggcagcatt gaagccaatc ttcatcagct tccaggcaaa gttttgcgcc
     3061 accagacccg aagcgccaat accggtcaga attatccgcc gcgcagagcg caacattgtt
     3121 acgcattcat gcagtttctc ttcactatta acgtttagcg ttgcgtacat agcggcagta
     3181 ttttctttaa tcagtttttc gccgaccagc cgtaacggat catcaccgcg gatttggtta
     3241 tgaatgggca cggagggcga ttccggctgg cttgccagcg cttcactcaa cgccaactta
     3301 agcgccggaa aacctttata gccgagtttt tgcgcgaact tcacgacgct ggactgactg
     3361 actccggctt cgttggccag ttgctgagag cttaaatggc gcgccgtatc aggttgtagc
     3421 agcagataat ccgccagttt tttatcactc tgggcaagcc cctggtaacg ctgacggata
     3481 cgaagtaagc cgttcatatc tttcctcgcg atgaaatagt ggcgacaaca ggcaatatga
     3541 gacacacgcg agtgaatttt atattccatt gccgctaatt gccatgaatt aaaaattcca
     3601 gttagccatg atgcggtcga ttgttgccgg aaaagtgatg atctatctgt taggctatgt
     3661 agtagcgaaa attaatcagg atgtttcagt ccagaggagt atggtttggc aactcacgag
     3721 cgtcgtgtgg tgttttttga cttagatgga acattgcatc agcaggatat gttcggcagt
     3781 tttctgcgct atttactgcg tcgccaaccg ctgaatgcgt tacttgtcct gccgttgtta
     3841 ccgattatag ccattgcgtt attgataaaa ggtcgtgcgg cacgctggcc gatgagtctg
     3901 cttctgtggg ggtgcacttt tggtcacagc gaagcacgtt tacagacgtt gcaggccgat
     3961 ttcgtgcgct ggtttcgcga caatgttacc gcctttccgc tggttcagga gcgattaacc
     4021 acctacctgt taagttccga tgctgatatc tggttgatta ccggctctcc gcagccgctg
     4081 gttgaagcgg tttatttcga tacgccctgg ctgccgcggg ttaatcttat cgccagccaa
     4141 attcagcgtg gctatggtgg ttgggtattg acgatgcgtt gtctgggaca tgaaaaggtc
     4201 gcacaactgg agcgcaaaat cggcactccg ctgcggctgt acagtggcta tagcgacagt
     4261 aatcaggaca atccgctgct ttatttctgt cagcatcgtt ggcgagtaac cccgcgcggt
     4321 gaactccagc aactggaata gagtaaagca tagcgtccgt gtataatgcg ccgcgctttt
     4381 ataaccggag ttttcttttt gtctgaagtc gaatttagcc acgaatactg gatgcgtcac
     4441 gcgctgacgc tggcgaaacg tgcctgggat gagcgggaag tgccggtcgg cgcggtatta
     4501 gtgcataaca atcgggtaat cggcgaaggc tggaaccgcc cgattggtcg ccatgatccc
     4561 accgcacatg cagaaatcat ggccctgcgg cagggtggtc tggtgatgca aaattatcgt
     4621 ctgatcgacg ccacgttgta tgtcacgctt gaaccatgtg taatgtgtgc cggagcgatg
     4681 atccacagtc gcattggtcg cgtggtcttt ggtgcgcgtg acgcgaaaac tggcgctgcg
     4741 ggatctttaa tggatgtgct gcatcatccg ggtatgaatc accgagtgga aattacggaa
     4801 ggaatactgg cggatgagtg cgcggcgttg ctcagtgact tctttcgcat gcgccgccag
     4861 gaaattaaag cgcagaaaaa agcgcaaccc tcgacggatt aattttgttt ctcttcactc
     4921 cctttcctgg aaaacagcag agagggagaa tgggtcaaat agtttgatga cgactgggaa
     4981 agaaacgaga gaaaaggaaa tttctctttg cccaactccg taggcgatac cgccggataa
     5041 tcctgcgcca gttgcatcgc cgcttctgta gcctgcttct ctttctcttg cagataaccc
     5101 accaggctaa tctgatactt acgaatattt tcgacataag cgtaggcttc atgtccacga
     5161 gcgtagccgt aagtcagctt gctgtaatag ggtttctggc taagtaaagg cagacgctgt
     5221 tttacgtcag cccaactgtc aggattccct ttggtttttg ccgtcagggc gcgggcatcc
     5281 agcatatgcg catagcccat attgtacgca gcgagggcaa accagatccg ctcgttctcc
     5341 ggcacacttt ccggcacttt actcatcata tcctgcaaat aacgcacgcc accgctgatg
     5401 ctctgttcgg catcggtacg atccgtaatg ccgaggcttt gcgcggtatt tttggttaac
     5461 atcatcatgc cgcgcacacc cgtcggtgaa gtggcctgtg catcccagtg cgattcctga
     5521 taagcaatag cggccagcaa acgccagtca atttcttcgg cgtatttctc aaacaggggc
     5581 tttaactgcg gcagtaccgc atcgacggcg cgtaaaaatg tgcgcgtatc gacgtaatca
     5641 aaatcatcgc catgccccag gtatttctct tcaatgcgtg ccagcgtaca gtcttcattc
     5701 atttcgttga agaagtcgag cagggcggcg gaaagggtat tatcgccatc taacgggcta
     5761 aaccaagtca ccggttgttc atcggtgata tcgagcgcta cggcgagctc cgggtgaacg
     5821 cgctgaaaca ggctgatggc gacagaatca gcaatggtgt aatcgagttt tccttcgatg
     5881 acatcttcca ttaattccgc agagcctttt ttgtcgtcta ccttccagct taattccggg
     5941 aattttgtgt ctttcagggt ttggagatcg ttaaccacca cgtgacccgg tgcaacggtg
     6001 agttgctccg ccgtcaggtt gcccagtgtg cgcgggcgat actgactcac tttataaacc
     6061 agttgttgtg acacggaata ataggtaggg ccaggctgat aattttttac ccgctcactg
     6121 ttatagacca gtcctgccgc cagcaggtcg gcgttaccat tatcgaggtc gtcaaacagc
     6181 tggctgatat tctgccgcac ggtcactttc agttttacac cgaggtaatc ggcaaactgt
     6241 ttcgccagtt cgtaatccag gccaaaaggt ttcccgttga tttcgttata agtcagggga
     6301 gtatgaatgg tgctcacacg caactctccc cgcgcttgaa tggcggcgat acggttgtcg
     6361 gctttaccaa accagggaat ggatggccag agagcgaccg cgagcagcag tgccagaatg
     6421 ccgatgaaca gataattaat ctttaatttt ttcaattagt taattctctg tgtcgtgcgc
     6481 gtcccagctt gaaaaaacgt aataatagtg aaaggtttac tcataaatga gcggcatttt
     6541 gcgtaaacct gcgccagatg gcaacttatt acagccattg gcggcacgcg ttgctaattc
     6601 acgatggtga ttttatttcc acgcaaacgg tttcgtcagc gcatcagatt ctttataatg
     6661 acgcccgttt cccccccttg ggtacaccga aagcttagaa gacgagagac ttatgatgga
     6721 aattctgcgt ggttcgcctg cactgtcggc attccgaatc aacaaactgc tggcacgttt
     6781 tcaggctgcc aggctcccgg ttcacaatat ttacgccgag tatgtccatt ttgctgacct
     6841 caatgcgccg ttaaacgatg atgagcacgc acaacttgaa cgcctgctga aatatggccc
     6901 ggcactcgcc agccacgccc cgcaaggcaa actcctgctg gtgaccccgc gtcctggcac
     6961 catctctccc tggtcttcga aagcgaccga tattgcccat aactgcgggc tacaacaggt
     7021 aaaccgcctt gagcgcggcg ttgcttacta tatagaagcc ggtacgctga ccaatgaaca
     7081 atggcagcag gttaccgctg aactgcacga ccgcatgatg gaaacggtct tttttgcttt
     7141 agatgatgca gagcagttgt ttgcccacca tcaaccgact ccggttacca gcgttgattt
     7201 gctggggcag ggccgtcagg cgctgatcga cgctaacctg cgtcttggcc tggctctggc
     7261 ggaagatgaa attgactatc tgcaggatgc tttcacaaag cttggtcgta acccgaacga
     7321 catcgagctg tatatgttcg ctcaggcgaa ctctgagcac tgccgccaca aaatttttaa
     7381 tgccgactgg attatcgacg gtgaacagca gccgaaatcg ctgttcaaga tgatcaaaaa
     7441 tactttcgaa accacgccag atcacgttct ctctgcttat aaagataacg ccgccgtaat
     7501 ggaaggttct gaagtgggcc gctactttgc tgaccacgaa acgggccgct acgatttcca
     7561 tcaggaaccg gcgcatattc tgatgaaagt cgaaactcac aaccacccga cggcgatttc
     7621 tccgtggccg ggcgcggcga cgggttccgg cggcgaaatt cgtgatgaag gcgccaccgg
     7681 acgcggtgcg aagccgaagg ctggtctggt tggtttctcc gtttccaacc tgcgaattcc
     7741 tggcttcgaa cagccgtggg aagaagattt cggtaagcct gagcgcattg tcaccgcgct
     7801 ggacatcatg accgaaggcc cgctgggcgg cgcggcgttt aacaacgaat ttggtcgtcc
     7861 ggcactgaat ggctacttcc gtacttatga agaaaaagtg aacagccaca acggcgaaga
     7921 gctgcgcggt tatcacaaac cgatcatgct ggcgggcggg atcggcaaca ttcgcgccga
     7981 tcacgtacaa aaaggcgaga tcaacgtcgg tgcgaagctg gtcgttctcg gcggcccggc
     8041 aatgaacatc ggtcttggcg gcggagcggc gtcttccatg gcgtcaggtc agtctgatgc
     8101 cgacctcgac tttgcttccg tacagcgcga caacccggag atggagcgtc gctgccagga
     8161 agtgatcgac cgttgctggc agcttggtga tgccaaccca atcctgttta tccacgacgt
     8221 cggcgctggc ggtctttcta acgcgatgcc ggaactggtg agcgacggcg ggcgcggcgg
     8281 taaatttgaa ctgcgcgata ttctaagcga cgaaccgggc atgagcccgc tggaaatctg
     8341 gtgtaacgaa tcccaggaac gctacgtgct ggcggttgct gccgatcaat taccgctgtt
     8401 tgacgaactg tgtaagcgtg agcgcgcacc ctacgcggtg attggtgaag cgaccgaaga
     8461 actgcatctt tctctgcacg atcgtcattt tgataatcag ccgatcgatc tgccgctgga
     8521 cgtcctgctt ggtaaaacgc cgaagatgac ccgcgatgta caaacgctga aagcgaaggg
     8581 cgatgcgctg gtccgtgaag ggatcaccat tgcagacgcg gtgaaacgcg tactgcatct
     8641 gccgaccgtg gcagagaaaa ccttcctggt gaccatcggc gaccgcagcg taaccggcat
     8701 ggtagcgcgc gatcagatgg tggggccgtg gcaggtgccg gtcgctaact gcgcggtcac
     8761 taccgccagc ctcgacagct actacggtga agcgatggcg attggcgagc gtgcgccggt
     8821 tgcactgctg gatttcgccg cctctgcccg tctggcggtc ggtgaagcgt taaccaatat
     8881 tgccgcaaca cagattggcg atatcaaacg cattaaactt tccgccaact ggatggcggc
     8941 ggcaggccac cctggtgaag atgcgggcct gtatgaagcc gttaaagccg tgggcgaaga
     9001 gctttgtccg gcgctgggcc tgacgatccc ggtgggtaaa gactctatgt cgatgaaaac
     9061 ccgctggcag gaaggtaacg aagagcgcga aatgacgtcg ccgctgtcgc tggtgatttc
     9121 tgcatttgcc cgcgtggaag atgtgcgtca caccatcacg ccacagcttt ctaccgaaga
     9181 taacacactg ctgctgattg atttgggcaa aggcaataac gcgctgggcg caacggcgct
     9241 ggcgcaggtt tatcgtcagc ttggcgacaa accggcagat gtacgcgacg tcgcgcaact
     9301 gaaaggcttc tatgacgcga ttcaggcgct ggttgcacag cgtaagctgc tcgcatacca
     9361 cgaccgctct gatggcggcc tgctggtaac gctggcggaa atggcctttg ctggtcattg
     9421 cggcattaac gcggatattg cctctctggg cgacgatcgc ctggcggcgt tgtttaacga
     9481 agaactgggt gcggtgattc aggttcgtgc cgctgaccgt gaagcggtcg agtccgtact
     9541 ggcacagcat gggcttgctg attgtgtcca ttatgtaggg caggcggttt ccggtgaccg
     9601 ttttgtgatt accgccaacg ggcagactgt attcagcgaa agccgcacca cgttgcgtgt
     9661 ctggtgggca gaaactacct ggcagatgca gcgcctgcgt gacaacccgg agtgtgctga
     9721 ccaggagcac caggcgaaat caaacgacgc cgatccgggc ctgaatgtga aactgtcgtt
     9781 cgatatcaac gaagatgtgg cagcaccgta tattgccact ggcgcacgtc cgaaagttgc
     9841 tgtgctgcgt gagcagggcg tgaactcgca tgttgaaatg gcggcagctt tccaccgtgc
     9901 aggctttgat gctatcgacg tgcatatgag tgacctgctg accggacgca cgggcctgga
     9961 agatttccac gccctggtcg cgtgcggtgg tttctcctac ggtgatgtgc tgggtgccgg
    10021 tgaaggttgg gcgaagtcaa tcctgttcaa tgaccgtgta cgcgatgagt ttgcaacctt
    10081 cttccaccgt ccgcaaacgc tggcgctggg ggtatgtaac ggttgccaga tgatgtctaa
    10141 cctgcgtgaa ctgatcccgg ggagcgagtt gtggccacgt tttgtgcgta acacctccga
    10201 tcgctttgaa gcgcgtttca gcctggttga agtaacccaa agcccgtctc tgctgttgca
    10261 ggggatggtg ggctcgcaaa tgccgattgc tgtctctcat ggtgaagggc gcgtggaagt
    10321 gcgtgatgcg gcgcatctgg cggcactgga aagcaaaggg ctggtggcac tgcgctatgt
    10381 cgataacttc ggcaaagtca ctgaaaccta cccggctaac ccgaacggtt caccgaacgg
    10441 tattacggcg gttacgactg aaagtggtcg agtcaccatt atgatgccgc acccggaacg
    10501 tgttttccgt actgtcagca actcctggca tccggaaaac tggggcgagg atggcccatg
    10561 gatgcgcatt ttccgcaatg cgcgtaagca gttggggtaa gtcatcagtt cattggtttt
    10621 gcagcccgga tgcggcatta agcaccaacc actatcataa gctcccaact ccgggagctt
    10681 ttttgtgtct gtaaatcacg acaatgggtg gtttaccgtg tcgctttctg gcgacactta
    10741 aatgattgat tttattgtaa tctaataagt ttatcttaag ttgtctcttt ttagcgacac
    10801 agtggctgat ttacattcaa atagcgaaga agccataaat ccctcaaagc aacgtataat
    10861 cagtacgtta ccaaactatt ttctttattg gcacagttac tgcataatag taagcagtgg
    10921 ctcattcacc gacttatgtc agccccttcg ggacgtgcta cataaaattc gaatgacgca
    10981 caacaaggtg cctgccgtcc aacttctgat atcagcgtag ctatatcaac catcgggcga
    11041 aacgtcgagt taggcaccgc cttattccat aacaaagccg ggtaattccc ggctttgttg
    11101 tatctgaact tcccctcggt tagcatcagg ctattcgcgt ctgacgagag taacaccttg
    11161 aaacgctggc ccgtttttcc ccgctcatta cgacaactgg taatgctggc atttttgcta
    11221 attctactac ccctgttggt gctggcatgg caagcctggc aaagcctgaa tgcgcttagc
    11281 gatcaggcgg cgctggttaa ccgcactacg cttattgatg cccggcgtag tgaagcgatg
    11341 accaacgcgg cgctggagat ggagcgtagc taccgtcagt attgcgtgct ggacgaccct
    11401 acgcttgcga aggtttatca aagccagcgc aagcgttaca gcgaaatgct cgatgcccac
    11461 gcaggcgtac tgccagacga taaactttac caggcattgc gtcaggattt gaacaatctg
    11521 gcccaacttc agtgtaacaa cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc
    11581 tttgccagtg ccaataccga aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg
    11641 cagcaacttc agcgtgaaat cgccgaacgt gggcaatatt ttggttggca atcgctggtg
    11701 ctatttctgg tgagtctggt gatggtgcta cttttcacgc ggatgattat cgggccggtg
    11761 aaaaatatcg agcgcatgat caaccgtctg ggggaagggc gttctctggg caatagcgtt
    11821 tcgttcagtg gaccgagcga gttacgctcg gttgggcaac gtattctttg gttaagtgag
    11881 cgcctgtcat ggctggaatc ccaacgccat caatttttaa gacatttatc tcatgaatta
    11941 aaaacgccac tggcgagtat gcgcgagggc actgaattac tggctgacca ggttgtcggg
    12001 ccgcttacgc cagagcaaaa agaggtggtg agcattcttg atagcagcag ccgcaatttg
    12061 caaaaactga tcgaacaact gcttgattac aaccgtaaac aggcggacag tgcggtggaa
    12121 ctggagaatg ttgaattagc accgctggtg gagacagtgg tttctgctca tagcctgccc
    12181 gcacgggcta aaatgatgca taccgacgtc gatctcaaag caacagcttg cctggcggag
    12241 ccaatgctgc tgatgagcgt actggataat ctttactcca atgcggtgca ctacggggct
    12301 gaatccggta acatttgcct tcgcagcagt tcacatggtg cgcgggttta tattgatgtc
    12361 atcaatacag gcacgcccat tccgcaagag gaacgcgcca tgatcttcga accctttttt
    12421 cagggaagcc accagcgaaa aggggcggtg aagggcagcg gtctgggatt aagcattgcc
    12481 agggattgta ttcgccgtat gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac
    12541 gtttgtttcc gcattgaatt accgtcgtcg aaaaacacga aataatgcta acagcctatc
    12601 ctatcaggct attttatttg tcagttcggc cccgggcagt gctcgaaatc ttcacgtacg
    12661 atatgtacgc tccggtttct ccgcgctgtc catgtccaga ctgtctgcaa caattacgcc
    12721 cactgggata ggctttaagt ctggtgaata tgcgacacat ttttcaacga ttattgcccc
    12781 gacggctgtg gctggcaggg ctgccatgtc tggcattgct gggttgcgtg cagaatcaca
    12841 ataagccagc cattgatacg ccagcagaag aaaaaattcc ggtttatcaa ctggctgact
    12901 atctttctac tgaatgtagc gacatctggg cgctgcaagg taaatcaacc gaaaccaatc
    12961 cgctttactg gctgcgggcg atggattgtg ctgatcgttt aatgcctgcg cagtcgcgtc
    13021 agcaagctcg ccaatatgac gacggcagct ggcaaaatac ctttaaacaa gggattttgc
    13081 tcgccgacgc caaaattacg ccgtatgagc gccgccagct ggtcgcgcgt attgaagcgt
    13141 taagtaccga gatcccggca caggttcgcc cactgtatca actctggcgc gacggtcagg
    13201 cgctgcaact gcaactggcg gaagagcgcc aacgttacag taaactccag cagtccagcg
    13261 acagcgagct ggatacattg cgccagcaac atcacgttct acagcagcaa ctggaactca
    13321 ccacccgcaa gctggaaaac ctgaccgata ttgaacgcca gctctcgacc cgcaaaccga
    13381 ctggaaattt ctcgccggat acgccgcacg agagtgaaaa gcccgcgcca tccacccatg
    13441 aggtcactcc tgatgagcca taaacctgcg catttattat tggtcgatga cgatccggga
    13501 ttgctgaaac tgcttggcct gcgcctgacc agcgaaggct acagtgtggt cacggcggaa
    13561 agtggcgctg aaggattacg ggtactgaat cgcgaaaaag tagatttagt tatcagcgac
    13621 ctgcggatgg atgaaatgga cggtatgcag ctatttgctg aaatccagaa agtgcagccg
    13681 ggaatgccag taattattct tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca
    13741 cagcagggcg tttttagttt cctcaccaag cctgtcgata aagatgcgct atatcaggca
    13801 attgacgatg cgctggagca atccgcgcca gccaccgatg aacgctggcg cgaggcaatt
    13861 gtcacccgca gcccgctgat gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca
    13921 gacgtcagcg ttttgattaa cggtcagagc ggcaccggga aagagatttt cgcccaggct
    13981 atccacaacg ccagcccgcg caacagcaaa ccatttattg ctattaactg tggcgcattg
    14041 cccgagcaat tgctggagtc ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct
    14101 gtcagcaatc gcgaaggttt attccaggcg gcggaaggcg gaacgctatt tctcgatgaa
    14161 attggcgata tgcctgcgcc gttacaggtc aaactgctgc gcgtgttgca ggagcgtaaa
    14221 gtgcgcccgc tgggcagtaa ccgcgatatt gatatcaatg tgcggattat ttctgccact
    14281 caccgtgatc tgccaaaagc gatggcgcgc ggggaattcc gcgaagatct ctattaccgc
    14341 ctcaacgttg tcagcctgaa aattccggca ctggcggagc gcacagaaga cattccgcta
    14401 ctggcaaatc acctgttgcg ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc
    14461 tctaccgatg cgatgaaacg cctgatgacc gcgagctggc cgggtaatgt gcgccagttg
    14521 gtcaacgtga ttgaacagtg cgtggcgctg acctcatctc cggtgattag tgatgcgctg
    14581 gtggagcagg cgctggaggg tgaaaatacg gcgctgccaa cctttgttga ggcacgtaat
    14641 cagtttgaac tcaactattt gcgtaagctg ctgcaaatca ccaaaggcaa cgtcacccac
    14701 gcggcgagaa tggcggggcg caaccggaca gaattttata aactgctttc ccgacacgag
    14761 ctggatgcaa acgatttcaa ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa
    14821 agacaggcga ccttttcaag gaatagcatg aaaaagattg atgcgattat aaaacccttc
    14881 aagctggacg atgtccgcga agcactggcc gaagtcggta ttaccggcat gacggtgacc
    14941 gaagtgaaag gctttggtcg ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat
    15001 atggtggatt ttctgccgaa agtgaaaatt gagattgtcg taccggacga cattgtcgat
    15061 acctgtgtcg ataccattat tcgcacggcg caaaccggca aaatcggtga cggtaaaatc
    15121 ttcgtctttg acgtggcacg ggtcattcgc atccgtaccg gtgaagagga cgacgcggca
    15181 atttaaaccg cgtcaagggt tgccggatgt ttccatccgg caacatcaaa ttacagcacc
    15241 ttatgcgggc caaagcattc gtaatgaatg ttttcctgct tcacgcccag atccactaac
    15301 tgtttcgcgg taaactgcat gaagccaacc gggccgcaga gatagaactg cattgtcgga
    15361 tcgctgaacg caccttccag tttgctcaaa tccatcagac cttcgctatc aaactgacct
    15421 ttagcgcgat cggcttcgct cggctgacga tgccaggtgt gcgcggtaaa gcgcggcagt
    15481 gactgcccca gttccttaac ttcatcggca aaggcgtgaa catcgccatt ttctgccgca
    15541 tggaaccagt tcacttgtgc tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc
    15601 attggcgttt gaccaacacc ggcagagatt aacgtcactg gtgtgtcatc tgcgacagcc
    15661 ataaagaaat cacctgccgg agcgaccagt ttcacgacat cgccaacatt ggcgtgattg
    15721 tgcaaccagt tggatacctg cccaccctct tcgcgtttca ccgcaatacg atagcctttg
    15781 ccatccggtt tgcgagtcaa agagtactga cgaatttcct gatgcgggaa accttccggc
    15841 ttcagccaga cgccgagata ttgccccgga cggtattctg ccactgcgcc accgtcgacc
    15901 ggctccagtt cgaagctggt gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa
    15961 tcgcgagtac cttcccaacc accggctttg ctggcgtttt cgttatagat ttccgcctcg
    16021 cgattgataa atacattagc cagtacacca taggctttac cccacgcgtc cagcacttcc
    16081 tgccccgggc tgaacatttc gtccagcgtt gccaacaggt gttcaccgac gatgttgtac
    16141 tgttccggtt tgatctggaa gctggtgtgc ttctgcgcga ttttttctac cgctggcagc
    16201 agcgcaggca ggttttcaat attactggcg taggcggcaa tagcgttaaa cagggcttca
    16261 cgttgatcgc cattacgctg gttactcatg ttaaaaattt ctttgagttc tgggttatga
    16321 gtaaacatac ggtcgtagaa atgggcggtt aactttggcc ccgtttccac cagtaaaggg
    16381 atggtggctt ttactgtagc gatggtttga gcgtcaagca tatggtcttc ctttttttgc
    16441 atcttaattg atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt
    16501 ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa
    16561 gaaatccgtt tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa
    16621 tcgtttgcgt aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt
    16681 cgccgttgtc caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg
    16741 agatgcggat gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg
    16801 ctatggagca ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact
    16861 acaccagccc gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag
    16921 gttatccggg caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg
    16981 cgatcgatcg tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg
    17041 gctcccaggc taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg
    17101 gtatgaacct ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta
    17161 aactgtacaa catcgttcct tacggtatcg atgctaccgg tcatatcgac tacgccgatc
    17221 tggaaaaaca agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt
    17281 ccggcgtggt tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttacctgt
    17341 tcgttgatat ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc
    17401 ctcatgctca cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc
    17461 tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc
    17521 ctggtggtca gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag
    17581 aagcgatgga gcctgagttc aaaacttacc agcagcaggt cgcgaaaaac gctaaagcga
    17641 tggtagaagt gttccttgag cgcggctaca aagtggtttc cggcggcact gataaccacc
    17701 tgttcctggt tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg
    17761 gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg
    17821 tgacctccgg tattcgcgtg ggtactccgg cgattacgcg tcgcggcttc aaagaagcag
    17881 aagcgaaaga actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg
    17941 ttatcgagcg catcaaaggt aaagttctcg acatctgcgc acgtttcccg gtttacgcat
    18001 aagcgaaacg gtgatttgct gtcaatgtgc tcgttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaac tttgcaaatt caatatattg caatatccgt gtaggcctga
    18121 taagcgtagc gcatcaggca atcttaggta tgtgatcatc aaggcttcct tcgggaagcc
    18181 tttctacgtt attgcgccat caaatctgtc gtaactgcgc ctcaacatac aaatagccaa
    18241 tccccaacac ctgttgtgcg cggcttaatt gcccaaagcc aatttgcgtt gctttcacgc
    18301 tcggcccttc gtcgcggtcg aacgggttaa atcccgtctg gcgaataatg gtattaagcc
    18361 agtgctcacc aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt
    18421 tgaggaagtt atacagactc aaaccaatgg cattggcact gcgatcaacc caactggtga
    18481 tccacggttc tccactctac caggcagcga ttaactgcgc tgtagtcagt ttttccggat
    18541 caagctgagt attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcac
    18601 ttaacgaggc gacggtttcc aggcagccgt aacgtccaca gtcgcagacg acgccatccg
    18661 gattaacgat ggtgtgacca atctgtccgc taccatacaa actgccgcga taaatttgct
    18721 cgttaatcac gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt
    18781 tctgcgaatt attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca
    18841 tcacccgaat gccgagcttc tcttccagca gatacttcac ctcaaccggc gttgtccacg
    18901 gcgcttgcgg catggtttgc gacacgccgg tcaccggatc aacctgaccg tggattgcca
    18961 gcgccagatt gatggtgcgg tccggccaca atttacggtg gcgatgccag catttttcga
    19021 tttcggacag cagcgcctgc ggcgtcggtg catcaatctg taaatactca aattcacctt
    19081 tcggacttaa acaagcatta gcaacctgac actcaatact ggtgggcgtc acgttcaggc
    19141 acagcgtcca gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct
    19201 ggctttcatc gtcgatattc accacccgtt tttcgctttc cagttcctgc aaaatattac
    19261 tgactgccgg aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt
    19321 gccggtacag cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattgttaa
    19381 tgcaggctct catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac
    19441 attgccagat tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat
    19501 tgagggcacc atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga
    19561 aaccgggccg caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc
    19621 cggcgcggtt tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc
    19681 ctggcaggcg gtgtggctgg aaaacgacta catcaaagtg atgatcctgc cggaactggg
    19741 cggtcgggtg catcgcgcgt gggataaagt gaaacagcgc gattttgttt atcacaatga
    19801 agtcattaaa cctgcgctgg tggggctgct ggggccgtgg atttccggtg ggattgagtt
    19861 taactggccg caacatcatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc
    19921 ccatgaagac ggtgcacaga cggtgtgggt aggcgaaacg gagccgatgc atggtttaca
    19981 ggtgatgaca ggtttcaccc tgcgccctgg gcggcgctgg aaatcgccag ccgcgtctat
    20041 aacggcaacg ccacgccgcg tcatttcttg tggtgggcca acccggcagt gaaagggggt
    20101 gaagggcatc agagcgtttt cccaccggat ttgactgcgg tgtttgatca cggcaaacgg
    20161 gccgtctccg ctttccccat cgctaccggc acttactaca aagtggacta ctccgctgga
    20221 gtggacattt ctcgctataa aaatgtgccc gttccaacct catatatggc cgaaaaatcg
    20281 cagtacgatt ttgtcggcgc gtggtgtcac gatgaagatg gcggtttgct gcacgttgcc
    20341 aaccaccata ttgcgccagg taaaaaacag tggagttggg gacacagtga atttggccag
    20401 gcgtgggata agagtctgac cgacaataac ggcccgtata tcgaactgat gaccggtatt
    20461 tttgccgata accagcccga ttttacctgg cttgatgctt acgaagagaa gcgttttgag
    20521 cagtatttcc tgccttatca ttctctgggc atggtgcaaa atgcctcccg cgatgcggtg
    20581 ataaaactcc agcgtagtga gcaggggatt gagtgggggc tgtatgccat ctctccgttg
    20641 aacggatacc gcctggcgat ccacgaaatc ggcaaatgca acgcgttact tgatgatgcc
    20701 gtggcactga tgcctgcgac cgccatccag ggcgtgttgc acggtatcaa tcctgatagg
    20761 ctgaccattg agctttccga tgccgacggc aatattgtac tgagttatca ggaacatcag
    20821 ccgcaagcgt tgccgttacc ggacgtcgcc aaagcgccac tggcagcaca agacattacc
    20881 agtacagatg aagcctggtt tatcggtcag catctggagc aatatcatca cgccagccgt
    20941 tcaccgttcg attactacct gcgcggcgtg gcgctggacc cgctggatta tcgctgtaac
    21001 ctggcgctgg cgatgctgga atataaccgc gcagatttcc cgcaagcggt ggcgtatgcc
    21061 agtcaggcgc tgaaacgcgc acatgcgctg aacaaaaacc cgcagtgtgg acaagcaagt
    21121 ttgattcgcg ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc
    21181 tggcgtgcgg tctggagcgg caacagcaaa gccggtggct attatggcct ggcacgactg
    21241 gcggcgcgta atggtaactt cgacgctggt ctggattttt gccaacaaag tcttcgcgcc
    21301 tgcccaacca atcaggaagt gctttgcctg cataatctgc tgctggtgtt aagtggtcgt
    21361 caggacaacg cgcgtttgca gcgcgcgaaa ctgctgcgtg attatccgct gaacgccatt
    21421 ctgtggtggc tgaactggtt cgatggtcgt agcgaatcag ccctcgcgca gtggcgcggt
    21481 ctgtgtcagg gacgcgacgt taacgccctg atgaccgccg ggcaactgat taactgggga
    21541 atgcccactc tggcgacaga gatgctgaac gcactggact gtcagcgcac gttatcgctt
    21601 tatctacaag ccagcttgct gccgaaagcc gaacgcggcg aactggtcgc aaaagccatt
    21661 gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag
    21721 agtattgaag agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgc
    21781 agctacaaca aagccattac tttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc
    21841 gatggctggc gcgggttggc gatccatgcg tggaataagc aacacgatta tgaactggcc
    21901 gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa
    21961 cgggatttgc tggataagtt aagtggagcc acaccggaga aacgactggc gcgtctggaa
    22021 aataatctgg aaattgcgct gaaacgcgac gacatgaccg cagaactgct caatttgtgg
    22081 catctcacgg gtcaggcaga caaagcggcg gacattctcg ccacgcgcaa attccacccg
    22141 tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc
    22201 tggcagcatc ttgatgccag acagccgcag caggcctgcg aactgcttca tgccgcgctg
    22261 cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg
    22321 ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgac gcgttgttta
    22381 cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg
    22441 gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acaaatcgca
    22501 cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag
    22561 gcggatttct ttgctgtttc acaacctgac ctgttgtcgc tgtatggcga tttacaacag
    22621 cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt
    22681 gcgcaatatg aatctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg
    22741 gcattattca ccaccgtgat gccttttatt tttaactacg ttcactaaac cctattaact
    22801 gtaatatttg agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt
    22861 aataaaaact ctacaggagg cactatgcct aaaaaaatga gaacaacccg taatctatta
    22921 ttaatggcta cgctattagg tagcgcacta tttgccaggg ctgcggataa agaaatgacc
    22981 attggtgcaa tataccttga taccctggga tattacgctg gagtgcgtca gggcgttcag
    23041 gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat
    23101 atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga tgccattatt
    23161 ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt
    23221 attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg
    23281 gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac tgggtaacgc tgccgccgat
    23341 tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt
    23401 gaagtttgtg tgcagcgacg taaaggattt gaagaagtat taaaatcccg cgttcccggc
    23461 gcgcaaattg tcgctaatca ggaagggact gttttaaata aagcgatttc cgttggtgaa
    23521 aaactgatta tctccacgcc ggatctcaac gccattatgg gggagtcggg cggtgcgaca
    23581 ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt
    23641 tcggatatga caaccgaaat tgctcaggag ctggaaaaca atcaggtgct gaaagcggta
    23701 gtggatattt ccggtaagaa aatgggcaat gctgttttcg cgcaaacatt gaaggttatc
    23761 aataaacaag ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg
    23821 gaagatggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc
    23881 agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa
    23941 taagcgttac cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga
    24001 agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac
    24061 cggcagcgaa cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga
    24121 cgaagctacg ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggaatt
    24181 aagtctggtg gaagggctga cggtggcaga aaacctctgc cttggtcaat ggcctcgccg
    24241 caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct
    24301 gggcgttgac gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact
    24361 ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacccac
    24421 cagctcgctt gccagtgcag aagttgaact ggtgatcagt gcggtgaaaa agatgtcggc
    24481 actgggcgtg gcggtgattt atgtcagcca ccggatggaa gaaatccgcc gcattgcctc
    24541 ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc
    24601 cacgcatcat attgtgtcgc taatgctagg gcgtgatcac gttgatattg cgccggttgc
    24661 ccctcaggaa attgtggatc aggccgtact ggaagtccgt gcgttacgcc ataagcccaa
    24721 gctggaggat atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct
    24781 gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggagacgt atgaacaggg
    24841 cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca tgctgaaacg
    24901 cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct ggttgggcgt
    24961 tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggag tgctgcaatg
    25021 gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggccgctag
    25081 tagcgaaacg cccatcggca cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg
    25141 ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat
    25201 cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt
    25261 ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgtgaccgca tcctgttatt
    25321 acagcacggc acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc
    25381 cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc
    25441 attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa
    25501 tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc
    25561 tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat
    25621 tgccgcctgg gcgatgacgc tgattattat ctccggtgaa attgatgtca gcgttgggcc
    25681 gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc
    25741 gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct
    25801 gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg
    25861 cggaatgggg ctgtttatga ccaacgcact gccagtgccg attgacgaaa acgaggtgct
    25921 ggactggctg ggcggacaat ttctcggtgt gccagtatcc gcgctgatca tgatggtgtt
    25981 gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc gggcgctcgg tttttgccgt
    26041 tggcggtaat gccacggcgg cgcagttgtg cggtattaac gttcgtcggg tgcgcattct
    26101 tatctttacc ctttcgggat tattagcggc ggtgaccggc attttgctgg cggcacgcct
    26161 cggttccggt aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt
    26221 ggtcggcggt acggcacttt ccggcggtcg cggttccctg ttcggtacat tgcttggtgt
    26281 gctggtgatt acgctaatcg gtaacggtct ggtgctgctc ggtattaact cctttttcca
    26341 gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca
    26401 gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc ggcagggatg
    26461 aaaattaatc gtattgttaa tgagggattt atgaaaacga tgctggcagc ttatttacca
    26521 ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga cgccggggat taaccaggta
    26581 ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg tccactatat ctatcatcaa
    26641 cactgtgcca cggcggcggc acccgataaa ccgttatacc agggatttat caacggtcat
    26701 gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc gccattttaa agagggcgac
    26761 cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt
    26821 cctatctctt gtactggcaa aggaaaagcg gcttacggct ggcagcgtga cggcggtcat
    26881 gccgaatatc tgctggcgga agaaaaagat ctgatcctcc tgccggatgc gctgagctac
    26941 gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc
    27001 gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg
    27061 atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa
    27121 cgtctggcga tggcaaaaca gttaggggtg atggatcacg gctatttagc aaccaccgaa
    27181 ggtctgccgc agattatcgc cgaactcacc cacggtggcg cggatgttgc gctcgattgt
    27241 tctggtaatg ccgcaggtcg cttgctggca ctgcaatcca ccgctgactg gggacgggtg
    27301 gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct gatgcaccat
    27361 caacggcgga ttatcggctc ctgggtgacc agtctgttcc atatggaaaa atgcgcccac
    27421 gatttgactg actggaagct gtggccgcgt aacgccatta cccatcgctt ctcgctggaa
    27481 caggcaggtg atgcctatgc gctgatggcg agcggcaaat gcgggaaagt tgtgattaac
    27541 ttcccggatt aaggagttag cggatgacca tctatacctt atcccacggg tcgctaaagc
    27601 tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct
    27661 tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat
    27721 ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg gcgtgagtat caactgcaac
    27781 cgaatgttga gtgggatgcg cactatctac acggcgatgg ctggctgggc gagtggcaat
    27841 gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc
    27901 actatcgggt aagtcaggcg tttcatttaa cggcggatac gctgacggtg acgctctctg
    27961 tcaccaatca aggggcagag acgctgccat ttggtaccgg ctggcatcct tattttccgt
    28021 tgtcaccaca aacgcggatt caggcgcagg cgagcggtta ctggctggaa cgggagcagt
    28081 ggctggcggg tgagttttgc gagcagctac cgcaggaact ggattttaac cagctcgcgc
    28141 cgttgccgcg ccagtgggtg aacaatggtt ttgccggatg gaatggtcag gcccgcatcg
    28201 agcagccgca ggaaggttat gccatcatca tggaaacgac gccacctgca ccgtgttatt
    28261 tcatctttgt ttccgaccct gcgtttgata aaggatatgc gtttgatttc ttctgtctgg
    28321 aaccgatgag ccatgcgccg gatgatcatc atcgcccgga agggggtgat ctcattgcgc
    28381 tagcaccagg ggaatcaaca acttcagaga tgtcgttgcg ggtagagtgg ctgtaattta
    28441 ttaaatgccg gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag
    28501 aaattgcccc cggcccggta attgccaata gcaagaacgc gccagcgata ctgacattct
    28561 tccagaaatt aatcatattt ggcccaaccg catcgccggt catatcccag taatggtgac
    28621 caatcaccgc cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa
    28681 agccaagcac gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca
    28741 gcatcggcat tggcgcgccc aacgaggcca tatattggac cgtaccgtca aagcccatca
    28801 ttttgggaaa accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca
    28861 aaacggggcg ggcagctcca aaatcaaaat aacg
//
LOCUS       DAQSWQ010000047        28374 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.047, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000047 DAQSWQ010000000
VERSION     DAQSWQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.047"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..133)
                     /locus_tag="R8G18_002161"
     CDS             complement(<1..133)
                     /locus_tag="R8G18_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEE9795851.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="R8G18_002162"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="R8G18_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(703..2026)
                     /locus_tag="R8G18_002163"
                     /pseudo
     CDS             complement(703..2026)
                     /locus_tag="R8G18_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(2026..3642)
                     /locus_tag="R8G18_002164"
     CDS             complement(2026..3642)
                     /locus_tag="R8G18_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEE9795852.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3938..4654
                     /locus_tag="R8G18_002165"
     CDS             3938..4654
                     /locus_tag="R8G18_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEE9795853.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4651..6312)
                     /locus_tag="R8G18_002166"
     CDS             complement(4651..6312)
                     /locus_tag="R8G18_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HEE9795854.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="R8G18_002167"
     CDS             complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="R8G18_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HEE9795855.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="R8G18_002168"
     CDS             complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="R8G18_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HEE9795856.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7455..7527)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7767..8099
                     /locus_tag="R8G18_002169"
     CDS             7767..8099
                     /locus_tag="R8G18_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HEE9795857.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8101..9315)
                     /locus_tag="R8G18_002170"
     CDS             complement(8101..9315)
                     /locus_tag="R8G18_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HEE9795858.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9416..10480
                     /gene="cbrA"
                     /locus_tag="R8G18_002171"
     CDS             9416..10480
                     /gene="cbrA"
                     /locus_tag="R8G18_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HEE9795859.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10477..11814)
                     /locus_tag="R8G18_002172"
     CDS             complement(10477..11814)
                     /locus_tag="R8G18_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9795860.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="R8G18_002173"
                     /pseudo
     CDS             complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="R8G18_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="R8G18_002174"
     CDS             complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="R8G18_002174"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEE9795861.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="R8G18_002175"
     CDS             complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="R8G18_002175"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEE9795862.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="R8G18_002176"
     CDS             complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="R8G18_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HEE9795863.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15477..16133
                     /locus_tag="R8G18_002177"
     CDS             15477..16133
                     /locus_tag="R8G18_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795864.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(16179..16991)
                     /gene="yidA"
                     /locus_tag="R8G18_002178"
     CDS             complement(16179..16991)
                     /gene="yidA"
                     /locus_tag="R8G18_002178"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEE9795865.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(17106..17504)
                     /locus_tag="R8G18_002179"
     CDS             complement(17106..17504)
                     /locus_tag="R8G18_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEE9795866.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17744..20158)
                     /gene="gyrB"
                     /locus_tag="R8G18_002180"
     CDS             complement(17744..20158)
                     /gene="gyrB"
                     /locus_tag="R8G18_002180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEE9795867.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(20187..21260)
                     /gene="recF"
                     /locus_tag="R8G18_002181"
     CDS             complement(20187..21260)
                     /gene="recF"
                     /locus_tag="R8G18_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEE9795868.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(21260..22360)
                     /gene="dnaN"
                     /locus_tag="R8G18_002182"
     CDS             complement(21260..22360)
                     /gene="dnaN"
                     /locus_tag="R8G18_002182"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEE9795869.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22365..23768)
                     /gene="dnaA"
                     /locus_tag="R8G18_002183"
     CDS             complement(22365..23768)
                     /gene="dnaA"
                     /locus_tag="R8G18_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEE9795870.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            24065..24145
                     /locus_tag="R8G18_002184"
     CDS             24065..24145
                     /locus_tag="R8G18_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795871.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24375..24515
                     /gene="rpmH"
                     /locus_tag="R8G18_002185"
     CDS             24375..24515
                     /gene="rpmH"
                     /locus_tag="R8G18_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEE9795872.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24532..24891
                     /gene="rnpA"
                     /locus_tag="R8G18_002186"
     CDS             24532..24891
                     /gene="rnpA"
                     /locus_tag="R8G18_002186"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEE9795873.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24855..25112
                     /gene="yidD"
                     /locus_tag="R8G18_002187"
     CDS             24855..25112
                     /gene="yidD"
                     /locus_tag="R8G18_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEE9795874.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            25115..26761
                     /gene="yidC"
                     /locus_tag="R8G18_002188"
     CDS             25115..26761
                     /gene="yidC"
                     /locus_tag="R8G18_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEE9795875.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26867..28231
                     /gene="mnmE"
                     /locus_tag="R8G18_002189"
     CDS             26867..28231
                     /gene="mnmE"
                     /locus_tag="R8G18_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEE9795876.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6845 a         7646 c         7229 g         6654 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gtttaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggttact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtcc tggctatttt gccaatagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccgcgcctta tatccccgcc
      601 ctgagggacg gggttttacg gcgcaccgga taaatttggt tatgcgccgt cagccgtgct
      661 aaagaatacg gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca
      721 gtacgctttg ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg
      781 taccgttttc gataacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc
      841 gtccacgacc agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg
      901 gatatcgccg accaccagtg gttctggtcc gttgtgcccg accagacacg ggatctcgac
      961 catcgcttca tcatcaaagt tatggattgc cccgttgtta ggcacaatca gcaacatccg
     1021 ctcctgagtg ttgaaggcaa ttgccgccgc cagatcgacg atgtatgacg catgttcgtc
     1081 aatctccagc ttggccgccg ctgaatttcc tgccgctgta atggcgcggc aagcatcgaa
     1141 aacctgtttc tcgcgatgtt ccattcacct cattcgcgcg ggtatgttca gggttggaat
     1201 gctgaacaac ataatccggg aagagataat atttcagata ggtgttcggc aatgtatccg
     1261 ggtccagtgc ctggacatcc cgcgcttttg cataggtgtc gttccagctc gcttcagtat
     1321 gtgtatcttg ctgcggtgga acataaccgt gcttcgagac atattgccgc agctggggca
     1381 tcaggtcgtt gccctgcaaa tcttcaattg atgtccacca gccaaagtgg ttcaggccgt
     1441 agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac aatttgcgcc atccggcttt
     1501 caataccgat tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg
     1561 tggcttctgc gacaatggct gccgggttgg agtagttgag catccaggca tttggtgaat
     1621 atttttccat ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta
     1681 ttccgccggg tccgcaagtt tcctgaccaa caacaccgtg gcgcagtggg attttttcat
     1741 ccagttcgcg catcgggtac ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc
     1801 tgaacgccac ttcaggatcg gtggtgtaac taaacgcaat gtccggcgct ttctctttaa
     1861 ggatgacttt acaggcttcg gcaatcactt cctgccgcgc gccatcgtta tcataaaatt
     1921 tcagtgcacg aagcgggaaa cgctcctgat tcgccaggag catcaacacg atgcctggcg
     1981 taaaggtgct tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt
     2041 aatggcaaca ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcag
     2101 ctgggataca tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg
     2161 cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat
     2221 gcgtagacgc gtcgcacaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg
     2281 caggataccg gcagcttgat ccagctcttt ttttggctct gccgcggtgg tttggcctcg
     2341 cgaggctttg tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt
     2401 gcacatgttg aactgcagaa tcagggtgcg gaaaaccacg aagtacagca gggtaaagca
     2461 caacccaatg gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca
     2521 gttttgcggt aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag
     2581 atacatcacg gtcgacattg aggccgcaag cacagcgtgt accgcaaaca gcaacggtga
     2641 aatgaacagg aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt
     2701 tgcgggaatc agcaagcccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat
     2761 cgctaaagaa atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaaccgcc
     2821 ttccgggaac aacgatttca gcggctcggc gctcaaactg aactcttgca gatgctgcgc
     2881 ccagtacatc tgaatgccgc cttcaacagc tgccggaccc aagataaacg gtccgtagat
     2941 gaagtggtgt aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccagacccc
     3001 aagcgcaccc gccgaacgca ggaacgcttg cagagattca atccccgttt gtacttttgg
     3061 ccagccgagc aatgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac
     3121 ataagacgtc ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt
     3181 atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt
     3241 tttaatcccg gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac
     3301 gccgaagtag cttccccagg tcattcccat cgcgttgatg aaatagttcc aggtcaggaa
     3361 actcaccatc accgccagac aagcacgtcc ctgcgcttgc ttagcaaggc caatgggtaa
     3421 accgacagca aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat
     3481 gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg
     3541 gttttgcagc aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg
     3601 cgtaaacatc gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct
     3661 caattctgta agtaggaagc ctgattggtt atgcggcagg cctggagcaa ggataggaag
     3721 atgcgatcgg cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt
     3781 gggcggtaga gatctacgtt ttcgcgaaaa agtagacatc tgtgagcaca ggtacacagc
     3841 atcgaacaca tctttaaaaa aaagatgttt tttcaatcga ttaagcagaa cttgtgtgcg
     3901 cattacccgg gcttgcaggc aaaaaagaga tctagagatg atctacaaaa gcattgcgga
     3961 gcggttaaga attcgactta actccgcaga tttcacgcta aacagccttc ttccgggtga
     4021 aaaaaagctg gcggaagagt ttgcggtatc gcggatgacc atccgtaaag ccattgacct
     4081 gctggtagcg tgggggctgg tggtccgccg ccacggtagt ggcacttacc tggtgcgcaa
     4141 agatgtactg catcaaaccg ccagtctgac cgggctggtg gaggtgttaa aacggcaggg
     4201 aaaaactgtc accagccagg tgctgatttt tgaaatcatg cctgcgcctc cggccattgc
     4261 cagccagtta cggattcaaa tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt
     4321 ggaagggaaa ccgctgatgc tggaagacag ctatatgccg gtaaaactgt tccgtaatct
     4381 ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt gaacaagagt gcgggatttt
     4441 gattggcggt aattatgaaa gcctgacgcc ggtgctcgcc gatagactgc tggcgcggca
     4501 aatgaaggta gcggaacaca cgccactgct gcggatcacc tcgttgtcat atagcgagag
     4561 cggggagttt ttgaattatt cagtgatgtt cagaaatgcc agcgaatacc aggtggagta
     4621 ccatttacgg cgactccacc cggaaaagag ttaaccgata ctccagaaga gcaccgccag
     4681 taattggggg gtgataatgc gcaggaacat cactaacgga tagacagtgg cgtaagagag
     4741 cgccgccgca ccgctagttg gatgaagatt attagcaaac gccagcgccg gaggatcggt
     4801 catggaacct gccagcatcc cgcacatggt caggtaattc attttggcta acatccgcgc
     4861 cagaatgcca acagtaatca gcggaacggc ggtgatcagg gcaccataac caatccagct
     4921 tagcccttcg ccattgacca gggtattcac aaaatcccca ccagatttca gaccaacgac
     4981 cgagaggaac agcacgatcc ccagctcccg cagcgcgagg ttggcacttg gcggcataaa
     5041 ccagtacagc ttgccgatac tgccgatacg cccgaggatc aacgccataa tcagcagacc
     5101 gcccgccagc cccagtttca acgcggccgg gaatcccggc acaaagacgg gaatagaacc
     5161 taacaatacg ccaagcccga tgccaataaa cactggcagc atctgaacct gttgcagttt
     5221 ttgctgcgca ttccccagca cattggcaac ggcatcaatt gcggacggac gccccaccag
     5281 gttgaggata tcgccgaact gcaggctgat atcgccactg gcgaccagtt cgaccccggc
     5341 acggttcagg cgcgagataa caacgtcata gcgttcttta aagtgcaggt cgcgaatacg
     5401 ttttccgagc acgttttcat tggtgaccac cacacgctcg acgcgcaaat cagtgtcttt
     5461 cgtggacagc gaagtatcga cctcctgacc aatcaccagt tgcgcattat gtaaatccgc
     5521 tggctgaccc accagatgca gcaaatcgcc cagttggata atggtatctg gcgaaggaac
     5581 ttttagggtt tcttcgcgtt tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg
     5641 tacatcttta atggctaaat catgcaggtt agggttctca acgcgaatat tgatagtctt
     5701 gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg tctcgacatt
     5761 gacgcggaaa ataacccgca acatccacat ggtgaacaaa atcccgcaaa tgccgaatgg
     5821 atacgccatc gcgtaactca tccccatctg atcgaccatt tccattggtg tacccaggtc
     5881 gcgcaaaatc tgctgccctg cccccagcgc tggcgtattg gtaaccgcac cggagaaaat
     5941 ccccagcact accggcagtg gaatatcaaa cagtttatgc aggatggcgg taaccagacc
     6001 accgatgatg acgatcagaa cagcaaacag gttgaggcgt aatccggaga cgcgcaatga
     6061 ggcaaagaag cccggcccta cctgaatccc gatggtataa acgaacagga tcaggccaaa
     6121 ttcctgaata acatgcagca tatcgctact taatgtcatc cccgcctgag aaacaaaatg
     6181 gccgacgatg atcccaccaa acagcacgcc gccaatacct aatcctatgc cgcgaaattt
     6241 gacgttgccg ataaacaaac cgacgactgc caccaaagcc agaatactga ccgttaatgc
     6301 tatatcactc atcgtccatt tctcttgaga taacatttta attgctacgg attatggctg
     6361 aatcggtgaa gcagatttgc cttatagcgc acattattac gggaaagaac gtgccgaaat
     6421 atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa agccccacca
     6481 gaatggcggg gcaaagagaa tagctagtta gctatttaac gcgggacgtt cgctgatagc
     6541 gatacgctgc gctgcgatgg gttcaggctc attacgaatt aaatcaatat gcagtaaacc
     6601 gtttacgaaa gttgcgccag agacttccat attttcagcc agcgtaaagc tcaggctaaa
     6661 tggctgattc ataagccctt gatgcagcca ttttttctct tcttttggct gctccggcgt
     6721 gccttttacg ctcaggcgcg taccttccag ttgaatctct aaatcttcct gacggaaacc
     6781 tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg
     6841 gaagctctga ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca
     6901 ttgacgcatc agtggggata aatcgaagtt acgcatagtc atttctcctt ctaagaagcg
     6961 agtaagtacc tgcaaatccg aagattcgca tatgctccct gacggcgagc atggagatgt
     7021 caggccgcgc caggcggcct taaggaatta gttgatttcg atacggcgcg gttttttcgc
     7081 ttccggaatc acgcgttcga gatcgatata cagcaaacca tttaccaggt tagcgccacg
     7141 aacatgaatg ttctcagcta actggaattt gcgttcaaag ttgcgttcag cgatgccctg
     7201 gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca ccagcagatt
     7261 atcctgggcg gtaatttcca gttcgctctc agcaaaacca gccacagcga tagcaatgcg
     7321 gtaatggttt tcgtctacca gttcaacgtt atacggaggg tagccgccat tactctggct
     7381 ctggttgttt tctaagtggt taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg
     7441 ggataaatca aagttacgca taatcaatag ctcctgaaat cagcgagaat gtaagacctt
     7501 ccacaatgga caggtcaggt agccagaaca cccatcaggc gcgtcctcat cgactacgat
     7561 gtaaaaatgg gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt
     7621 tatggtctat tgcatagact gagggggcag cacgaaacgt taaagtgcga tagccgccac
     7681 agagcgacgt aatgatgatg ttattttcag caagggatcg ctataactca tcaggccaac
     7741 acccacgatc aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat
     7801 gcagcggaat ggcattactc ggaggatgct ccagcgtgat gtcccatacc ggagggaaag
     7861 aaggaacgta tcccggcacc cgcgccagcg ccaccatgat tggcgatgat gaaaccaact
     7921 ggggcactaa gtcgctggcg attctcgata tgccgtttac cgctgtgatg gacacgcttt
     7981 tattaccgtg ggatgtgttc cgcaaagaca gctcggtgcg ctcacgcgtt gaaaaaagcg
     8041 aagccaacgc gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat
     8101 ttagcgatca gtttcggtga tccacagctg gccaccttcc atccacgcca gccattgacc
     8161 atccggcgag aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata
     8221 ctcaacctcg ccgctttgcg catgggcaca agcaattcga ttatccagca caaagcccaa
     8281 ccattctcct gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa
     8341 ctggcgcggc tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc
     8401 acgcattaaa aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac
     8461 gttgactaac cccggataag cccgatggtg ggtaaaggtt aaacgtcgct gcacgacgcc
     8521 acgcggtggc gcgggcaggg tggtttccgt tccacttaac ggcgcatcac ctgccgcttt
     8581 ccagccagct tcatcttgcg gcaactcaac gataaacagc tccggcactt tctcaccctt
     8641 tggcgaaagt gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata
     8701 agcacgattg atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac
     8761 acaccagtgg ctgccgctgt attcacgcgg atgctgcttt tgtacgttga ccggaccaaa
     8821 cggcgcagca acaccgacgt ttcgcaaatc cagcgccgga tcgagttcat gcattacatg
     8881 gtcgttatag gtaaagctca ccctttcacc gttcgggcta aagacatgca catggctgcc
     8941 gccgcgcagc gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac
     9001 cttgccgcct tcagcaatga ctccgcgccg atgatggaag tcgtaatgcc atgtttcatc
     9061 aggattttcc gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac
     9121 gccgacgtgt gcgccctgtg acgcacagta gataacctcg acctcaccgg tatggatatt
     9181 cacacgctca atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag
     9241 ccactggctg tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa
     9301 ggttatctgt ttcatggcgc ggtcctgata caaaacgggt ccagccatca tacttcacaa
     9361 ggacttcact ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga
     9421 acatttcgac gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt
     9481 agccggcaaa atgcaggtga tcgcgctgga taaaaagcac cagcatggta ctgaaggttt
     9541 cagcaaacct tgtggcggtc tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg
     9601 gctgacgctt cctgtcgatg tgatcgctaa tccgcagatt ttcagcgtca aaaccgtcga
     9661 cgtcgccgca tcgctcacgc gtaactacca gcgaagctat atcaatatta atcgccatgc
     9721 tttcgacttg tggatgaaat cgctgatccc cgccagcgtt gaggtttatc acgacagcct
     9781 gtgtcgaaaa atctggcgtg aggatgataa atggcatgtc atttttcgtg cagacggctg
     9841 ggagcagcat attaccgccc gctatctggt cggtgccgat ggtgccaact cgatggtgcg
     9901 gcgacatctc tacccggatc atcaaatccg taaatatgtc gcaatccagc agtggttcgc
     9961 agagaaacat ccggtgccgt tctactcctg catctttgat aatgcgataa ctgactgtta
    10021 ttcatggagt atcagcaaag acggttattt tatctttggc ggtgcctatc caatgaaaga
    10081 cggtcagacg cgtttcacga ctctgaaaga gaaaatgagc gcctttcagt tccagtttgg
    10141 caaagcggtg aaaagcgaaa aatgcacggt gctgtttccc tcacgctggc aggattttgt
    10201 ctgcggtaag gacaacgcct ttctgattgg tgaagcggcg ggatttatca gcgccagctc
    10261 gctggagggg attagctatg cgctggatag cgcagacatt ctgcgttcgg tgttactgaa
    10321 gcagccagag aagctcaata ccgcttactg gcgcgccacc cgcaaactgc gtttaaaact
    10381 cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa agtggattat
    10441 gcgcagtggt gtggcacata ttccacagtt gaaggattag ccaacgcgct tcacatcgcc
    10501 caccagcagg atataagaga gcgcgccaat caacgcgacg gcggagatat aaaccagtgc
    10561 aggtgcgaaa ccgtaaccct gcgccaggta ccccaccacc agcggaacgg tgatgccgcc
    10621 cagaccaccg gcgaagttaa acacgccgcc ggttaaacca atcaggcgca tcggtgccag
    10681 agaagaaacc agcgaccagg taatcgaagc aaaaccgtta ccgaagaatg ccagcgccat
    10741 caggcacata atcatcatcg gatcgttagt gtagttagcg cccataatgc aggtggagat
    10801 cagcaagccg cagataatcg gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac
    10861 cagcagatcc gctacccagc cagagagcag gacgccgaca aacgccgcga ggaatggcac
    10921 cgtggtcata aagcctgctt tcagcgccgt gattcctttt tcctgggtta aatagttcgg
    10981 gaaccaggtt aagaaaaacc acagtgtaga agccaccgca aattgcccaa gatagacgcc
    11041 gatcagttta cgatggaaca ccagtttcca gtctttggct gttaacggct gacgtgcctc
    11101 tttcttcact ggcgcatcac catccaccag accgccgcca tcacgaatgt aatccagttc
    11161 agctttgctg atgcctttgg tcaggcgcgg cggctgataa accttaaacc aaatcagcga
    11221 ccagataatg ccgataccac cagtcacaat gaacacccag tgccagctca acatctcctg
    11281 aatccagatc agcagcggag tcaaaaacgc cagaccgaca aactgaccag acgtataaaa
    11341 accaacggcg gaagcgcgtt catgttccgg gaaccagctg gtcaccatcc ggttattggt
    11401 cgggaaagcg ggcgcttcga aaataccggt tatcgcacgc agaccaatta atgacattaa
    11461 tcccgtggca aagccctgga ataaagtcgc cactgaccag ccaaatatcg caataaaata
    11521 agtcacgcga gaacctacgc gatctaaaaa ccaaccgccg gggatctggc atagcgtata
    11581 aagccaggcg aaggccgaaa atacatagcc catttccgct ttcgtaatgc cgaactcttc
    11641 ctgaatatgg gcggaagcca cggccaggtt agcgcggtca acataacaaa tgaccaccgt
    11701 aataaagatc atcaccagcg tcagataacg ccgacgcccc ggctttgctg cattaacggg
    11761 aatatccata gcgagctttc tccagatttt gggcatagcg aagccgctca ccatgccctg
    11821 taatttacag agggttatat ttttgtattg ctgttttagt gcccgatgag gggcttacgt
    11881 ggcaggaatt accactctgc tacgctgtta tcttcatgac gccagagtgg attacgccag
    11941 tccggggcat ttttactgaa ctcaataact ttagcttcgt cgatttccac gcctaagccc
    12001 ggtttcgtta gcggtttaaa gaagccgcca accatgctga agtcttcttt gtttttcaca
    12061 aagtcgagta actccgcgcc tttgttgtaa tgaattccca tactttgttc ctgaagtacc
    12121 gcgttatagg aaacaaagtc gatatgcaga caagccgcca gtgcaatcgg tccgagcgga
    12181 cagtgcggcg caagggtcac gtcataggct tctgccattc cggcaatttt gtagcattcg
    12241 gtaataccgc ctgcatggga gagatccggt tgtagaatcg aaataccgcc tgcctccagc
    12301 acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt
    12361 tgtgccgcca atttcgggta gtattcggct tgttccgcca gcaccggctc ttcaataaac
    12421 agcgggcgat acggctccag ttctttaatc agcactttcg ccattggcgc gctgacgcga
    12481 ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg
    12541 gtgttgactg ccgcatctac cgcgcgggag ttatcaatta gccccagttc ttcacaaccg
    12601 ttcagtttga aggtatcgaa gccgatttcg cgcagggttt taataccgtc gatcacatcc
    12661 gccggacgat cgccgccgac ccaactgtag gctttaattt tgtcgcgaac caggccgccc
    12721 atcagttgcc agaccgacgc attcagcact ttacctttga tatcccataa cgcctggtcg
    12781 atcccggcaa tggcgctcat caggattgga ccgccacgat aaaatccggc gcgatacatc
    12841 acttgccata agtcattgat gcgctatgga tcctgaccaa tcaaatagtc acccagctcg
    12901 tgaactgccg cttccaccgt gcgggcgcgg ccttcaatca ctggctcgcc ccagccgacg
    12961 acgccttcat cggtttcaat tttcaggaac atccagcggg gaggtaaacg atacgtggta
    13021 attttggtga ttttcattgc actgcctctc gatacgcctt aacaaatgct gctgcctgct
    13081 gcgcggtgcg ttctacggac tgcccggcgc gatagagatc gctgcctaag cccgccccag
    13141 cacaacctgc gtctatccac tgcgccaggt tttctggcgt cacgccgcca acggcaaaga
    13201 ctgcgatgtc cgatggcaat accgctttta acgctttgat gtattgcgga ccaaaagccg
    13261 atgacggaaa tattttcagc gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg
    13321 ccgtcgcgca gccggggcag acggtcatgc cgtagcccac cgcacggcgg atcacttcac
    13381 tatggatatt gggcgtaacg atgagctgac agcccatcct ggcgagcgca tcgacctgtt
    13441 caggtttcag taccgtacct gcgccaatca acgccttgtc gccatacgca tcaacgatgg
    13501 cgggaatgct ttgctcccat tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg
    13561 cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg cttaatgccg cgcaaaatgg
    13621 cgatcagcgg aagtttagtt tgccactgca tgagcgatgc tccttatacc agcctgaaat
    13681 gccgtgtcgc ccgccaccgc cgtcacgtcg caacccatcg cctgaaaggc ttgctggtag
    13741 cgcgcggtca acgatgttcc ggcgacaagg gtgatggcgt gttgatgggc cacatagtcg
    13801 cgcatactgg cgacctctgc gccaatcaac aaaccagaga gaaattcact gacctgttca
    13861 cggggaagtg ttcccagcac atgcgaggca cgaacttcaa aaagctgcgg caatatggcg
    13921 ggcgtattaa gaccacgctc aaggccagcc gtgaaggcat cggcagagtt ttcctgcggc
    13981 ggcaaacctg caccaatcaa tgagtgattt aacagtaaat gatgtaattc accggtcatc
    14041 acggtgcgaa aatcgttgat ttgctggcta tcggcctgca cccatttgca atgcgtaccg
    14101 ggcatgacat aaagagagga aggagccaga gctcgcgcgc cgatcaattg agtttcttcg
    14161 ccgcgcatca cattgtggtt atcatcatga gagacgcata atccgggaat aatccagata
    14221 ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa aacgggcagg aacagataaa
    14281 tacggagcaa ctttccagcc gacattgctg ccgaccattc ctgccattac cactggcgta
    14341 ttctcttcac gccagtcggt cgtgacttct gctaacaccg cagccggaga ttttccgttc
    14401 aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc agtggtcgcc ctgataaagc
    14461 caggcgcgca gattggtcga tccccagtca attgcgatgt agcgagctgt catgtgattt
    14521 cctttaacct tcgtgtcgag ctggcgatca tggtaagcgc cgcctgctct gccgcatcgc
    14581 cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt
    14641 tggcctcatc gcccatccag gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa
    14701 tgctaagttg ctgtaacacc gggttatgca ccgactgcag caccgcctcg tggtagcgaa
    14761 tatccgcttc gttaaacgct tcgcggtcct gattgttggc aatcatctcg ttcagcgccg
    14821 attcaatctg cgccagatcg ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg
    14881 gttccaccag atttcgcact tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt
    14941 ccagcaccca ttgcagtacg tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa
    15001 acgcgccgcg ataacgtttc atttcaatca gccgtttcgc catcaacgaa cggaacacct
    15061 cacggatgat gttgcgcgag gttgcaaact cctcacagag ttccgcctca gccggaagcg
    15121 gcgagcctgg tacgtatttg ccgtgaacga tctgtttacc cagcgtaatg acgatgcgat
    15181 cggttttatt gagagtcatg gagagtcctt gtgctctgta tgttcttctc tactttaccc
    15241 cgatcgatgc ataacacggc aactttgtag taccagcgtg atgacgttcg cgtttgctgt
    15301 gcgtgtaatg tagtacaaac ttatattgtt gtactacaat ttagatcaca aaaagaacaa
    15361 tgcgtaaaaa aatgacatgc gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca
    15421 ggagggaaag aagagtatgc tttcaacggc ttagctactc gtttaaagga ttaatcatga
    15481 agttgaatct taagggattt tttaagactg ccggtttatt cccactggcg ctgatgcttt
    15541 cgggctgtat ctcgtatgcc ctggtttccc ataccgcaaa gggtagttca ggaaagtatc
    15601 aatcgcagtc agacaccatc acggggctat cgcaggcaaa ggatagtaat ggaacaaaag
    15661 gctatgtttt tgtaggggaa tcgctggatt accttatcac agatggtgcc gatgacatcg
    15721 ttaagatgct caaggatcca gctcttaacc ggcacaatat tcaggttgct gatgacgcaa
    15781 gatttgtttt aaatgcgggg aaaaagaaat ttaccggcac aatatcactc tattacaact
    15841 ggagcacaga agaagaaaaa gcacgagtga cacaatatcg ttttgcttgt ggtgttcagc
    15901 actgtacccg gacagtagaa aacctggaag gtactatcca tgagaaaaat aaaaacatgg
    15961 attactcaaa cgtaatggct ttctaccatc catttaaagt gcgattttat gaataccatt
    16021 cacccagagg cattccggat ggtgtttccg cagcattact gccagtgact gttacgctgg
    16081 acatcattac tgcaccgctg caatttctgg ttgtatatgc agtaaaccaa taatcagtaa
    16141 gcgggcaaac gcgtttatgc tgtttgcccg cccacagatt aattcagcac atacttctca
    16201 atagcaaacg ccacgccatc ttcaaggtta gatttggtga caaagttcgc cacttctttc
    16261 accgaaggaa tagcgttatc catcgccaca ccgacgcctg catattcaat cattgcgatg
    16321 tcgttttcct gatcgccaat cgccatgatt tcttccggtt taatacctaa cacgtcggcc
    16381 agtgatttca cacccgtacc tttgttaacg cgtttatcga ggatttcgag gaagtacggc
    16441 gcacttttca gcacggtata tttctctttc acttcctgcg gaatacgcgc gatagcctgg
    16501 tcgaggatgg cgggttcatc aatcatcatc actttcagga actgggtatt ggggtccatt
    16561 ttctccgctt cgcagaacac cagcggaata gtggcaacga aagattcatg cactgtgtag
    16621 tagctgatat cgcggttggc ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa
    16681 ccgacttcgc gagagagttt ttccaggaaa cgatagtcgt catagctgag agcagtttgc
    16741 gccacggtgc taccatcagc ggccttctgt actagcgcgc cgttataagt aatgcagtag
    16801 tcgcccggct gttccatatg cagctctttc aggtagttgt gcacacctgc atacgggcga
    16861 cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg cggcaatcgc atttttaacg
    16921 gcgggtgaaa tggtgtgatc gggcagcaga agggtgccat ccatatcgat agcaatgagt
    16981 ttgatagcca tgagttcccc aggtagattg gttcctgacc catgctaacg cgattccgct
    17041 caaaaatcag tacaacaccc gagggaaaag ggggatgcaa cgcgggttgc atgctcccca
    17101 tttgcttagc ggaagagttt ccctttcagc agttccatac ctgcggaaag cagatcgtta
    17161 ttggcttgtg gtgacacttc accttgcggt gagagcgcat caataatctt cggcaattgc
    17221 tctgccagta aactggaagc tgtactggta tccacgccaa gtttttgccc gagatcggac
    17281 accgcatttg tgccgagcgc cgattccagt tgctcgccac taaccgattg attgccctgt
    17341 tgattactca gccaggttga gagaatggcc cctaagccgc cactttgcag tttttccagc
    17401 agcacctgaa tgccgccctg ctcctcaacc caacttaaaa tagcctgata tttccccgca
    17461 tcgcctttca gaaaggcacc gacaacttca tcaaaaagcc ccatgataat cacctgtaaa
    17521 gcgttacgtg ttgacccaaa aagtatagat ttgcgggatg ataattgcgg attgcagaaa
    17581 taaaaagggc ggagatgatc tccgcccttt tcttatagct tcttgccgga tgcggcgtga
    17641 acgccttatc cggcctacaa aatcatgaaa attcaataca ttgcaagatt ttcgtaggcc
    17701 tgataagcgt agcgcatcag gcacgctcgc atggttagcg ccattaaata tcgatattcg
    17761 ccgctttcag ggcgttctct tcaataaacg cacggcgcgg ttcaacggcg tcgcccatca
    17821 gcgtggtgaa caactggtcg gcagcaatcg catctttaac ggtaacgcgc agcatacgac
    17881 ggctttccgg gtccatggtg gtttcccaca gctgttccgg gttcatctcg cccagacctt
    17941 tataacgctg gatggagagg ccgcgacggg actctttcac cagccagtcc agcgcctgct
    18001 cgaagctggc taccggctga cgacgctcgc cacgttcgat aaacgcatct tcttccagca
    18061 agccacgcag tttctcaccc agcgtgcaga tacgacgata ttcgccaccg gtgataaact
    18121 cgtgatccag cggatagtca gtatccacac cgtgggtacg cacgcgaaca atcggttcaa
    18181 acaggttttg ttcggcattg gtgtggacat caaacttcca ctggctgccg tgctgttctt
    18241 tgtcgttcag ttcgctgacc agcgcgttca cccagcgggt aacggtctgc tcatcagaga
    18301 ggtcggcttc cgtcagcgtc ggctgataga taagctcttt cagcattgct ttcggataac
    18361 gacgctccat gcggttgatc attttctgcg tcgcgttgta ctcagacacc aatttctcta
    18421 acgcttcgcc agccaatgcc ggtgcgctgg cgttggtgtg cagcgttgcg ccatccagcg
    18481 cgatagagat ctggtactga tccatcgctt cgtcgtcttt aatgtactgt tcctgcttgc
    18541 ctttcttcac tttgtacagc ggcggctgag cgatgtagac gtgaccgcgt tcaacgattt
    18601 ccggcatctg acgatagaag aaggtcaaca gcagcgtacg aatgtgcgag ccgtcgacgt
    18661 ccgcatcggt catgatgatg atgctgtgat aacgcagttt gtccgggttg tactcgtcac
    18721 gaccgatacc acagccaagc gcggtgataa gcgtcgccac ttcctgagaa gagagcatct
    18781 tatcgaagcg cgctttctca acgttgagga ttttaccctt cagcggcaga atcgcctggt
    18841 tcttgcggtt acgcccctgc ttcgcagagc cgcccgcgga gtccccttcc accaggtaca
    18901 gttcggaaag cgccggatcg cgttcctggc agtctgccag tttgcccggc aggcccgcta
    18961 agtcgagcgc acctttacgg cgggtcattt cacgcgcgcg acgcgccgct tcacgggcac
    19021 gggcagcatc gataattttg ccaaccacga ttttcgcgtc ggttgggttt tccagcaggt
    19081 attctgccag cagttcgttc atctgctgtt caaccgctga tttcacctca gaagaaacca
    19141 gtttgtcttt ggtctgggag gagaatttcg ggtccggcac tttcacggaa acgaccgcaa
    19201 tcaggccttc acgcgcatcg tcaccggtgg cgctaacttt ggcttttttg ctgtagcctt
    19261 ctttgtccat gtaggcgttc agggtacggg tcatcgccgc acggaagcct gccaggtgag
    19321 taccgccgtc acgctgcgga atgttgttgg taaagcagta gatgttttcc tggaagccat
    19381 cgttccactg caacgccact tcgacgccaa taccgtcttt ttcagtggag aagtagaaga
    19441 tattcgggtg gatcggcgtt ttgttcttgt tcagatattc aacgaacgcc ttgatgccgc
    19501 cttcatagtg gaagtggtct tctttgccgt cgcgcttgtc gcgcagacga atggaaacgc
    19561 cggagttgag gaacgacaac tcacgcagac gtttcgccag aatttcatat tcgaactcgg
    19621 tcacattggt gaaggtttcg aggctgggcc agaaacgcac catggtgccg gttttttcag
    19681 tctcgccggt aaccgccaac ggtgcctgcg gtacaccgtg ttcgtagatc tgacggtgaa
    19741 ttttaccctc gcgctggata accagctcca gtttttgcga cagggcgttt actaccgaaa
    19801 caccaacgcc gtgcagaccg ccggacactt tataggagtt atcgtcaaat ttaccgcctg
    19861 cgtgcagaac ggtcatgatc acttccgccg ccgatacgcc ctcttccggg tgaataccgg
    19921 tcggaatgcc gcgcccgtca tcctgtacag agacagagtt atcggcgtga atggtgacga
    19981 taatttcttt acagtgaccc gcgagcgctt cgtcgatagc gttatctacc acctcgaata
    20041 ccatgtggtg cagaccggtg ccgtcatccg tgtcgccgat atacataccc gggcgcttac
    20101 gcaccgcatc cagccctttc aggactttga tactggagga gtcataagaa ttcgacatca
    20161 acgtttctcg ctcatttata cttgggttaa tccgttattt tacccttttc cacggtaaac
    20221 atcttcgaat tttcgtccga catgtctata acgtgttcag cactgatcgc gctgacaaag
    20281 acctgtgatt gcgtcgcttt taagcggctg gcaagcagcc cgcgacgctc atcatcaagc
    20341 tcagaggcaa aatcatctat caggtagaga caccgccgcc cgctttcacg ggtgaggaac
    20401 tctccttgcg ccagacgtaa ggcgcacatc aacagcttaa gctgcccacg cgataaggta
    20461 tcttccaccg gcgcaccgtc ggcgcgaatg cgtaagtccg ctttatgcgg gccatgcgcg
    20521 gtgtaggtta gctggcgatc gcgttcaaaa ttacgttcca gcacctcagc atattctgtc
    20581 tctttctccc agccgcgctg gaaagagaaa gtcagagaaa actcagggag aaattgctta
    20641 caagtatcgg ccatatcagc cgcgataccg gcgctatact ccgcgcgcca ggtgctgatt
    20701 tgctccgcca gcgggatcag ctctttatcc cacgggcgta gctgttcgta acgtgtcacc
    20761 tggcgcagcg ccgcattgcg ctgcttgagc aatcgcttga gattgctcca ggcggtgaaa
    20821 aatccgggtt cgttgtgaaa gcatccccag tcgaggaatg ctcttctgta tttggggccg
    20881 ccgttgagta aagtaaaccc ttctggcgtt atcaactgca ttggcatcag gtgcgccagt
    20941 tccgcgacct tatgcccgtc tgtaccgtcg atgcggactt tgctgtcgcc ctgtttgtct
    21001 ttggttaagc caatcgctgt ctcgcgctct tcgccctgta atcgcccgtg gagaacaaac
    21061 gcctcctgct catggcgaat gacgcgacca atctgcaaac tgcgaaacgc ccgaccatgg
    21121 ccgagcgtat agatggcttc cagcacgctg gttttgccac tgccgttggc acctaccaga
    21181 aagttaaagc cgggagataa agcgagatcc gcggtttcaa tgttgcggaa atcgcggatc
    21241 aacaagcggg tgagggacat tacagtctca ttggcatgac aacataggca gccgactgtg
    21301 atgcggcatc ttcaatctgc acgctggaaa ccgaatcggt cagcatcatg cggacgtttt
    21361 cacatttcag cgcgttcaga acatccagca cgtagctgac gttgaagccg atttccatct
    21421 ccgcaccgct ataggtaacg tcgaggatct cttccgcttc ttcctgttcc gggttgttgg
    21481 cggtgatttt cagctggttt tcgctgacat aaagacgtac gccgcggaat ttctcgttag
    21541 agagaatcgc cgcgcgagca aacgcctgct tgagcagatc gcagccagct tccagatgtt
    21601 tgtccgggtt cttcggcaga acgcggcgat aatccgggaa gcgaccatcc accagtttgg
    21661 aggtgaagat aaagtcgcca acgtgggcgc gaatgttgtt gctgccaatc tgtacgcgca
    21721 gcggattgtc gccgccgtcg agcatacgca tcagttcaat cacgccttta cgcggcacga
    21781 tcaccgaatg gcttggcaaa gattgaccaa ttggcattga acagactgcc agacggtggc
    21841 cgtcggtcgc cacggtgcgc agttcttcgc cttcggtttc aaacagcata ccgtttaagt
    21901 aatagcgaac gtcctgatgc gccatagaaa actgggtcgc ttcaatcaga cgcttcatcg
    21961 tcgcctgcgg cagggtaaat tcgacttcgc tctgccagtc atcgaggttc gggaaatccg
    22021 ccgctggcag ggtagacagc gaaaaacggc tacgcccgga gcgcaccagc atccgttcac
    22081 cttccagctg tacggcaatt tccgcgcctt caggcagacc acggcagata tcaaagaatt
    22141 tgcgcgccgg aacggtcgtc gctcctggct cgtgtggctg aaccagcgca acgcgtgcca
    22201 ccatttccat ctcgagatca gtaccggtca gcgacaacgt accgtcagca acctgtaaca
    22261 gcagattacc gagaatcggt agcgtaggac gaccacctaa cggcccgctc acctgttgta
    22321 gcggttttaa taaatgctca cgttctacgg taaatttcat aggtttacga tgacaatgtt
    22381 ctgattaaat ttgaaaaatc ttctttgata tcgtggctct cttcacgcaa ctgctcgatc
    22441 ttacggcagg catgaagcac cgtcgtgtgg tcacgaccac caaacgcatc gccaatctcc
    22501 ggcagactgt ggttagtcag ctctttcgcc agcgccatcg ccatctggcg cggacgcgcc
    22561 accgagcggg atcgacgctt ggaaaggaga tccgcgactt tgatcttgta gtactccgcc
    22621 accgtcttct gaatattgtc gatggtgacc agtttttcct gcaatgccag caagtcgcgc
    22681 agtgcctcac gcacgaagtc gatggtgatc gcccgtccgg taaagttggc gttagcaata
    22741 acgcggttca gcgccccttc cagctcacgt acgttagatc gtagacgctt ggcgataaag
    22801 aacgccactt cgcctggcaa acgaatgtca ttttcgtcgg cctttttcat caggatcgcc
    22861 acgcgggttt ccagctctgg cggttcgatc gccacagtca gtccccaacc gaagcgggat
    22921 ttcaaacgat cctcaacgcc gttgatctct ttcggatagc gatccgaggt gagaatgatc
    22981 tgttgattac cttccagcag ggcgttgaag gtgtggaaaa actcttcctg agatcgttct
    23041 ttattagcaa aaaactgaat atcgtcgatc agcagtgcat ctacggaacg gtagtagcgt
    23101 ttaaactctt cgatcgcgtt gttttgcagg gctttaacca tgtcctgaac aaagcgctcg
    23161 gagtgcatat aaaccacttt ggcattcggc ttgcgcgcca taatgccgtt acccaccgca
    23221 tgcagcaggt gagttttacc cagacccgtg ccgccataaa ggaacaatgg gttataggca
    23281 ccgccagggt tatccgccac ctggcgagcc gccgcgcgcg ccagttggtt agatttacct
    23341 tcaacgaagt tatcaaacgt gtgtttgacg tttacgttag aacgataggt cggttctgcc
    23401 ggggccggga cgttatccca acctgagcgc gtagaaggcg cagcacgttg cggctgcgtt
    23461 tgcgccacct gtgcaggggc cgcgacgttg ctcgtcactg ccgcttgtgg cgtttgcgtc
    23521 accggtttgg tgccgacttc aaaacgcagc tgtggggcat ccgctccgca gaaactggtt
    23581 agcagtccat tgatattatt aaggtacttg tcccgtaccc aatcgaggac aaaacggttt
    23641 ggcgcgtaca gggccagcgt gttatcgctc agttccgcct gcaatgggcg tatccacata
    23701 ctgaattctg tggctggtaa ctcatcctgc aatcgggcaa gacactgctg ccaaagcgaa
    23761 agtgacacgg cggactccac tcgaacaaaa gtcgataatg actaaggctg aaacattcat
    23821 gattgttgac gtacgtcgaa aagaccctgc ttgagggtga cgcacgaacc gctgtctgcg
    23881 gttatatgcc cgatcaagat cctgcaaaac gatcgggacc gcggatcata gcctaaactg
    23941 cgcaagagat cttctgtttc tcacagattt tcccgattta tccacaggac tttccagacc
    24001 tcgctaagtg taaacgatcc tgccgcgagg cgggcacgat ttacgccgca tattggaaaa
    24061 tttaatgacc atagacaaaa attggcttaa tcgatctaat aaagatctag gacgatcctt
    24121 gcgctttacc catcagcccg tataatcctc cacccggcgc gccatgctgg ttttcactgg
    24181 tgtgaggtcg tacattttcc ctgcgaaaag gtgcggaaaa gcgcggtaaa taaggaaaga
    24241 gaattgactc cggagtgtac aattattaca atccggcctc tttaatcacc catggcttcg
    24301 gtgtccatcg tttcattttt cggcggatat ccaataaagc cattgaattt attcaagttt
    24361 aggtagaaat cgccatgaaa cgcacttttc aaccgtctgt actgaagcgc aaccgttctc
    24421 acggcttccg tgctcgtatg gctactaaaa atggtcgtca ggttctggca cgtcgtcgtg
    24481 ctaaaggccg cgctcgtctg accgtttcta agtaataaag ctaacccctg agtggttaag
    24541 ctcgcatttc ccagggagtt acgcttgtta actcccagtc aattcacatt cgtcttccag
    24601 cagccacaac gggctggcac gccgcaaatt accattctcg gccgcctgaa ttcgctgggg
    24661 catccccgta tcggtcttac agtcgccaag aaaaacgttc gacgcgccca tgaacgcaat
    24721 cggattaaac gtctgacgcg tgaaagcttc cgtctgcgcc aacatgaact cccggctatg
    24781 gatttcgtgg tggtggcgaa aaaaggggtt gccgacctcg ataaccgtgc tctctcggaa
    24841 gcgttggaaa aattatggcg ccgccactgt cgcctggctc gcgggtcctg atagccctca
    24901 ttcgggtcta tcaacgcctg attagtccgc tactcgggcc gcattgtcgt ttcactccaa
    24961 cctgttcaag ctacggaatt gaggcattgc gcaggtttgg agtgataaaa ggcagttggt
    25021 tgacggtgaa acgcgtatta aaatgccacc ctttacaccc tggtggtgac gatcccgtcc
    25081 cgcccggacc atttgatacc agagaacact aacgatggat tcgcaacgca atcttttagt
    25141 catcgctttg ctgttcgtgt ctttcatgat ctggcaagcc tgggagcagg ataaaaaccc
    25201 gcaacctcag gcccaacaga ccacgcagac aacgaccacc gcagcgggta gcgccgccga
    25261 ccagggcgta ccggccagtg gccaggggaa actgatctcg gttaagaccg acgtgcttga
    25321 tctgaccatc aacacccgtg gtggtgatgt tgagcaagct ctgctgcctg cttacccgaa
    25381 agagctgaac tctacccagc cgttccagct gctggaaact tcaccgcagt ttatttatca
    25441 ggcacagagc ggtctgaccg gtcgtgatgg cccggataac ccggctaacg gcccgcgtcc
    25501 gctgtataac gttgaaaaag acgcttatgt gctggctgaa ggtcaaaacg aactgcaggt
    25561 gccgatgacg tataccgacg cggcaggcaa cacgtttacc aaaacgtttg tcctgaaacg
    25621 tggtgattac gctgtcaacg tcaactacaa cgtgcagaac gctggcgaga aaccgctgga
    25681 aatctccacc tttggtcagt tgaagcaatc catcactctg ccaccgcatc tcgataccgg
    25741 aagcagcaac ttcgcactgc acaccttccg cggcgcggcg tactccacgc ctgacgagaa
    25801 gtacgagaaa tacaagttcg ataccattgc cgataacgaa aacctgaaca tctcttcgaa
    25861 aggtggttgg gtggcaatgc tgcaacagta tttcgcgacg gcgtggatcc cgcataacga
    25921 cggtaccaac aacttctata ccgctaatct gggtaacggc atcgccgcta tcggctataa
    25981 atctcagccg gtactggttc agcctggtca gactggcgcg atgaacagca ccctgtgggt
    26041 tggcccggaa atccaggaca aaatggcagc tgttgctccg cacctggatc tgaccgttga
    26101 ttacggttgg ttgtggttca tctctcagcc gctgttcaaa ctgctgaaat ggatccatag
    26161 ctttgtgggt aactggggct tctccattat catcatcacc tttatcgttc gtggcatcat
    26221 gtacccgctg accaaagcgc agtacacctc catggcgaag atgcgtatgc tgcagccgaa
    26281 gattcaggca atgcgtgagc gtctgggcga tgacaaacag cgtatcagcc aggaaatgat
    26341 ggcgctgtac aaagctgaga aggttaaccc gctgggcggc tgcttcccgc tgctgatcca
    26401 gatgccaatc ttcctggcgt tgtactacat gctgatgggt tccgttgaac tgcgtcaggc
    26461 accgtttgca ctgtggatcc acgacctgtc ggcacaggac ccgtactaca tcctgccgat
    26521 cctgatgggc gtaacgatgt tcttcattca gaagatgtcg ccgaccacag tgaccgaccc
    26581 gatgcagcag aagatcatga cctttatgcc ggtcatcttc accgtgttct tcctgtggtt
    26641 cccgtcaggt ctggtgctgt actatatcgt cagcaacctg gtaaccatta ttcagcagca
    26701 gctgatttac cgtggtctgg aaaaacgtgg cctgcatagc cgcgagaaga aaaaatcctg
    26761 attcggtgag ttttcgctaa aataagggcg gtcagttgac cgcctttttt cttttcgtag
    26821 ggtggataag caccgcgcat ccgccacaca aagcaacagg aacatcatga gcgataatga
    26881 cactatcgta gcccaggcca cgcctccggg acgtggcggc gttggcatcc tgcgcatctc
    26941 cggcctaaaa gcccgtgaag ttgccgaaac cgtgctgggt aaactgccta agccgcgcta
    27001 cgccgattat cttccgttta aagacgccga cggcagcgtg ctcgatcagg ggattgcgct
    27061 atggttccct ggcccgaact cgttcaccgg cgaagatgtg ctggaactgc agggtcatgg
    27121 cggtccggtg atcctcgacc tgctgttaaa acgcattctg accattcccg gcctgcggat
    27181 tgctcgccct ggtgagtttt ccgaacgcgc gtttcttaac gataaactcg acttagccca
    27241 ggccgaggcg attgccgatc ttatcgacgc cagttcggaa caggcggccc gttcggcact
    27301 taactcgctg caaggcgcat tctccgcacg ggtcaaccat ctggtggaag ccctcaccca
    27361 cttgcgcatt tacgtcgaag cggcaattga tttccctgat gaagagatcg atttcctctc
    27421 cgacggcaaa attgaagccc agcttaatga cgtgattgcc gatctcgatg cagtgcgtgc
    27481 tgaagcacgt cagggtagtt tgttgcgcga agggatgaag gtggtgattg ccggacgtcc
    27541 taacgccggt aaatcgagcc tgttaaacgc gctggcgggc cgtgaagcag caatcgtaac
    27601 tgatatcgcc ggaaccacac gtgacgtgct gcgtgagcat atccacattg acggaatgcc
    27661 gctgcatatc atcgataccg ctgggctacg tgaagccagt gacgaagtgg aacgtattgg
    27721 tatcgagcgc gcgtggcagg aaattgaaca ggccgaccgc gtgctgttta tggtcgatgg
    27781 caccacaaca gacgccgtgg atccggcaga gatctggccg gaatttatcg cccgtctgcc
    27841 agcgaaacta ccgatcaccg tggtgcgcaa taaagccgat atcaccggcg aaacgctggg
    27901 aatgagtgaa gtgaacggtc acgcgttaat tcgtctctcg gcaaggacag gtgaaggcgt
    27961 ggaggtgctg cgtaaccatc tcaaacagag catgggcttt gacaccaaca tggaaggcgg
    28021 cttcctggcg cgtcgtcgcc acctacaggc gctggaacag gcagcggagc atttgcagca
    28081 aggtaaagcg caactgttag gtgcctgggc gggtgaactg ctggcggaag agttgcgcct
    28141 ggcacagcag aacttaagcg aaatcaccgg ggaatttacc tctgatgatt tgctggggcg
    28201 gattttctcc agcttctgta ttggtaagta accgcgctta cgaagccgta ttctgactgt
    28261 cagatgcggc ttcgcttcat tgttagcact cctgttattc ctcaaccctt tttttaaaca
    28321 ttaaaattct tacgtaattt ataatcttta aaaaaagcat ttaatattgc tccc
//
LOCUS       DAQSWQ010000048        27658 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.048, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000048 DAQSWQ010000000
VERSION     DAQSWQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.048"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..807
                     /locus_tag="R8G18_002190"
     CDS             <1..807
                     /locus_tag="R8G18_002190"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEE9795877.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="R8G18_002191"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="R8G18_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HEE9795878.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="R8G18_002192"
     CDS             2629..3948
                     /locus_tag="R8G18_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HEE9795879.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4320..5702
                     /gene="dosC"
                     /locus_tag="R8G18_002193"
     CDS             4320..5702
                     /gene="dosC"
                     /locus_tag="R8G18_002193"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HEE9795880.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5727..8126
                     /gene="dosP"
                     /locus_tag="R8G18_002194"
                     /gene_synonym="pdeO"
     CDS             5727..8126
                     /gene="dosP"
                     /locus_tag="R8G18_002194"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HEE9795881.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8385..8966
                     /gene="ddpX"
                     /locus_tag="R8G18_002195"
     CDS             8385..8966
                     /gene="ddpX"
                     /locus_tag="R8G18_002195"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HEE9795882.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8980..10530
                     /locus_tag="R8G18_002196"
     CDS             8980..10530
                     /locus_tag="R8G18_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEE9795883.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10532..11554
                     /locus_tag="R8G18_002197"
     CDS             10532..11554
                     /locus_tag="R8G18_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9795884.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11551..12447
                     /locus_tag="R8G18_002198"
     CDS             11551..12447
                     /locus_tag="R8G18_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HEE9795885.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12444..13430
                     /locus_tag="R8G18_002199"
     CDS             12444..13430
                     /locus_tag="R8G18_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEE9795886.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13459..14355
                     /locus_tag="R8G18_002200"
     CDS             13459..14355
                     /locus_tag="R8G18_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEE9795887.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="R8G18_002201"
     CDS             complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="R8G18_002201"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HEE9795888.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15187..15402
                     /gene="bdm"
                     /locus_tag="R8G18_002202"
     CDS             15187..15402
                     /gene="bdm"
                     /locus_tag="R8G18_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HEE9795889.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15504..15641
                     /gene="sra"
                     /locus_tag="R8G18_002203"
     CDS             15504..15641
                     /gene="sra"
                     /locus_tag="R8G18_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HEE9795890.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15798..17495
                     /gene="maeA"
                     /locus_tag="R8G18_002204"
     CDS             15798..17495
                     /gene="maeA"
                     /locus_tag="R8G18_002204"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEE9795891.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17629..18631
                     /gene="adhP"
                     /locus_tag="R8G18_002205"
                     /pseudo
     CDS             17629..18631
                     /gene="adhP"
                     /locus_tag="R8G18_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            18777..19061
                     /locus_tag="R8G18_002206"
     CDS             18777..19061
                     /locus_tag="R8G18_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HEE9795892.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="R8G18_002207"
     CDS             complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="R8G18_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HEE9795893.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="R8G18_002208"
     CDS             complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="R8G18_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HEE9795894.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="R8G18_002209"
     CDS             complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="R8G18_002209"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(23292..23294),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEE9795895.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24111..24992
                     /gene="yddG"
                     /locus_tag="R8G18_002210"
     CDS             24111..24992
                     /gene="yddG"
                     /locus_tag="R8G18_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HEE9795896.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            25251..26351
                     /gene="ompC"
                     /locus_tag="R8G18_002211"
     CDS             25251..26351
                     /gene="ompC"
                     /locus_tag="R8G18_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HEE9795897.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            26515..26997
                     /locus_tag="R8G18_002212"
                     /pseudo
     CDS             26515..26997
                     /locus_tag="R8G18_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27112..>27658
                     /locus_tag="R8G18_002213"
     CDS             27112..>27658
                     /locus_tag="R8G18_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEE9795898.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT         6971 a         6896 c         6822 g         6969 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagaggg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggcttttttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct ggggccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc acgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttatctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcaaagac cttcttccct gacttctcta
     1561 aagtgggcac cctggttgta tttgttgcct tcattttgag ttatatgggc gtagaagcat
     1621 ccgcaaccca cgtcaatgaa atgagcaacc cagggcgcga ctatccgttg gctatgttac
     1681 tgctgatggt agcggcaatc tgcttaagct ctgttggtgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcacagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggct attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 actctaccga tatgtatgtt gaattactgg tcgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgctcgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcatatat ggatatctgc
     2641 tcccgaaacg agaaattagc gattagaaga ccagcgatac tggttgcact ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtg
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaaccccac cagccgggcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tgcaacgtct cggtataaac
     2941 acggtctttt tccaggttaa gccggacggt accgccctgt ggccatcgaa aattttgcca
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattt
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc agaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga gtcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgc aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaaag tatcgcacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgctgacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaaccg
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac tcccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
     4081 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
     4141 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtcct
     4201 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
     4261 aatctcgcac gttgcgtttt gattccgtct gatttcccag ccttataagg gtgtgaatta
     4321 tggagatgta ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa caggcagatc
     4381 cactcattcg tgctaaagcc gcggaaattg ccgttgcgca tgctcattat ctgagtatcg
     4441 agttttatcg aattgtccgc atcgacccgc atgccgaaga attcttgagt aatgaacaag
     4501 ttgagcggca gttgaaaagt gcgatggaac gctggattat taacgtgctt tctgcccagg
     4561 ttgacgatgt cgaaaggcta atacaaatcc agcataccgt cgcggaagtg catgcccgca
     4621 taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa atcctctatc
     4681 cggtaatctt ctcttcggat tattccgccg cagaaaaact tcaggtctat catttctcga
     4741 ttaacagtat tgatatcgcg atggaagtga tgacccgcgc gtttaccttt agtgacagta
     4801 gtgcctcaaa ggaagatgaa aattatcgta tcttctcgtt actggaaaac gccgaagaag
     4861 aaaaagaacg gcaaatagcc tcaatacttt catgggaaat agatattatc tataaaatcc
     4921 tgctggattc tgatttaggc agtagtttgc cattaagcca ggctgatttt ggcctgtggt
     4981 ttaaccataa aggtcgacat tattttagtg gcattgccga agtaggccat atctcccgtc
     5041 tcattcagga tttcgacggt attttcaatc aaaccatgcg taacaccagg aatttgaata
     5101 acagaagtct gcgggtgaaa tttttattac agataagaaa taccgtatcg cagattatta
     5161 ccttgctgcg tgaattgttt gaagaagtat cgcgccacga agtcggtatg gatgtactaa
     5221 cgaaattact taaccgccgt ttcctgccga ctatcttcaa acgcgaaatt gcccatgcca
     5281 accggaccgg tacaccgctg tcagtgctga ttattgacgt tgataaattc aaagagatca
     5341 acgatacgtg gggccataac actggcgatg aaattctgcg taaagtctct caggcctttt
     5401 atgacaacgt ccacagtagt gattatgttt ttcgctacgg gggcgatgaa tttatcattg
     5461 ttttgactga agcttctgaa aacgaaacgt tacgtaccgc agaacgtatt cgcagtcggg
     5521 tggagaaaac caaactgaaa gccgcaaacg gcgaggatat tgccctctca ctttccatcg
     5581 gtgccgccat gtttaatggt catcctgact atgagcgcct cattcaaata gccgatgaag
     5641 ctctgtatat cgccaaaaga cgaggtagaa accgtgttga actctggaaa gccagtcttt
     5701 agatgcgcca ggatgcagag gtaatcatga agctaaccga tgcggaaaat gccgccgatg
     5761 gcattttttt ccccgccctt gaacaaaata tgatgggcgc ggtgttaatt aacgaaaatg
     5821 atgaagtgat gtttttcaac cccgccgcag agaagctctg ggggtacaaa cgtgaagaag
     5881 tcattggcaa taacattgat atgctgattc cgcgggattt gcgtcctgcg catcctgaat
     5941 acattcgtca taaccgtgaa ggtggtaaag cgcgcgtcga ggggatgagt cgggagctgc
     6001 agctggagaa aaaagacggc agtaaaatct ggacccgttt tgcgctatcg aaagtgagcg
     6061 ccgaggggaa agtttattac ctggcgctgg tacgggatgc cagcgtagaa atggcgcaaa
     6121 aagaacagac ccgacaattg attattgccg ttgaccatct cgaccgaccg gtgattgtcc
     6181 tcgatccgga acgccatatt gtgcagtgca atcgcgcatt taccgaaatg tttggttact
     6241 gcattagcga agccagcggt atgcagcccg atacactcct gaacattcct gaattccctg
     6301 ccgataaccg cattcgttta caacagttgc tatggaaaac cgcccgcgat caggacgaat
     6361 ttctgctgtt gacacgcacc ggtgaaaaaa tctggattaa agcctctatc agcccggttt
     6421 atgacgtgct cgcgcatctg cagaacctgg taatgacctt ctcggatatc accgaagaac
     6481 ggcagatccg ccagcttgaa ggcaatattc tcgccgccat gtgcagcagc ccgccatttc
     6541 atgaaatggg ggaaatcatt tgtcgtaaca tcgaatctgt tctcaacgaa tcgcatgttt
     6601 cgctgttcgc actgcgcaac gggatgccga tacactgggc gtcatcttcg cacggtgcag
     6661 aagttcaaaa tgcgcaaagc tggtcagcga ccattcgtca gcgtgatggt gcgcctgcgg
     6721 ggatcctgca aattaaaacc tcgtcaggag cagaaaccag cgcctttatc gaacgcgtgg
     6781 cagatatcag ccagcatatg gccgcgctgg cgctggaaca ggaaaaaagc cgtcagcata
     6841 ttgaacaact catccaattt gatccgatga ccggtctgcc aaatcgcaat aacctgcaca
     6901 attacctcga tgaccttgtc gacaaagccg tctctccggt ggtgtatctc atcggtgttg
     6961 accatattca ggatgtgatt gatagccttg gctatgcgtg ggccgatcag gcattgctgg
     7021 aagtggtcaa tcgctttcgt gaaaaactca aaccggatca gtatctctgt cgtatcgaag
     7081 gtacgcagtt tgtcctcgtg agcctggaaa acgacgtcag taacattacc caaatcgccg
     7141 atgagctacg gaatgtggtc agcaagccga taatgattga cgataaacca ttcccgctaa
     7201 ccttgagtat tggcatcagc tacgacgtgg gtaaaaaccg cgattacctg ctctccactg
     7261 ctcacaatgc aatggattat attcgcaaga atggcggtaa cggctggcag ttcttcagcc
     7321 cggcgatgaa cgaaatggta aaagagcgtt tggttttagg cgcagcgctg aaagaagcga
     7381 ttagcaataa ccaactgaaa ctggtttacc agccgcaaat cttcgcagaa acgggtgaac
     7441 tgtacggcat cgaagccctt gctcgctggc acgatcccca gcatggtcat gtaccccctt
     7501 cacggtttat tcctctcgca gaagagattg gtgaaatcga aaatattggg cgctgggtca
     7561 tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca gaatattcat atcccggcgt
     7621 tatccgtgaa cttgtcggcg ctgcactttc gcagtaatca gctgcctaat caggtgtctg
     7681 atgcaatgca agcctggggt attgacggcc accagctgac ggtagaaatc acggaaagca
     7741 tgatgatgga acacgatacc gaaatcttta agcgcattca gatcctgcgc gatatgggcg
     7801 tgggcttatc ggtagatgat tttggcacgg gcttttccgg attatcccgc ttagtcagtc
     7861 ttccggtaac ggaaatcaaa attgacaaaa gttttgtcga tcgttgtctg acggaaaaac
     7921 gcatccttgc cttacttgaa gccattacca gcattgggca aagcctcaat ttaaccgtcg
     7981 tggcggaagg cgtcgaaacc aaagagcaat ttgagatgct acgcaagatc cactgtcgcg
     8041 ttattcaggg atatttcttt tcccgccccc tacccgccga ggaaattcca ggctggatga
     8101 gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc ccgcactcgc gggttttcat
     8161 ttaacgtgac actgtcactt aaatactgtg atttcagcta cgattccggg agattccttc
     8221 cttaacttcc cctcttgcac caaaactgca ctacgctgca caaatgttga tcaaaagtta
     8281 aaaaatataa ctttaagcca gcattctggc gcttatcccg gcatggcatg agatctgcat
     8341 aagcggaaag cgcagcaatt tttgtcttaa tacaggaacc gtttatgtcg gataccaccg
     8401 aactggttga tttagccgtg atcttccctg atctggagat cgaattgaaa tacgcctgcg
     8461 ctgataacat cacaggtaaa gctatttacc agcaagcgcg ttgtctgtta cacaaggatg
     8521 cgattaccgc gctggcgaaa agtatcagta tcgcccagct gtcaggctta caactggtga
     8581 tttacgatgc gtatcgccca caacaagcac aggcgatgtt gtggcaagcc tgcccagacc
     8641 cgcaatatgt tgttgatgtg acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc
     8701 tgacgcttcg tgatgagcac gggaacatcc tggatatggg cgcagggttc gatgaaatgc
     8761 acgagcgctc ccatgcctat cacccttccg tcccgcccgc cgctcagcgc aatcgcctgt
     8821 tgctgaatgc gataatgact ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact
     8881 tcgaattacc ccaggcagcg agttaccctc tgctcgctga tcaattctct tgttttatat
     8941 cccctggcac acagcacgtc agttaatcag gaaactgtca tgaagagatc gatatcgttt
     9001 cgtcccacat tgctcgcgct cgtccttgcc acaactttcc cggttgcgca cgccgccgta
     9061 ccgaaagata tgctggtgat tggtaaggcc gccgatccac aaaccctcga cccggcggta
     9121 acaatagata ataacgactg gacagtgacc tacccgtctt atcagcggct ggttcagtac
     9181 aaaacggacg gtgataaagg ctcaaccgac gttgaaggcg atctggcaag tagctggaaa
     9241 gcgtctgacg atcaaaaaga gtggacgttc accctgaagg acaatgctaa atttgccgat
     9301 ggcacacctg tcactgccga agcagtaaaa ctctcttttg agcggttact aaaaatcggc
     9361 caggggccag cagaagcatt tcccaaagat ttaaagattg atactcccga cgaacatacg
     9421 gtgaagttta cccttagcca accattcgca ccgttcctct acacgctagc gaatgacggt
     9481 gcatccatta tcaatccggc ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc
     9541 ctcgcgcaaa ataccgccgg ttccggacca tttatgctga aaagctggca aaaaggtcag
     9601 caattagttc tggtgccaaa tccgcattac cccggcaata aaccgaactt taagcgagta
     9661 tcggtaaaaa ttatcggtga aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt
     9721 gacattgccg atgcgctgcc ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc
     9781 aatgtggcag agtatccgtc actgcgcgtt acctatctgt atctgaataa cagcaaagcg
     9841 cctcttaatc aggcggatct gcgtcgggcc atttcctggt ctaccgatta tcagggcatg
     9901 gttaacggca ttctgagtgg taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg
     9961 tggggctacg atgcgacggc aatgcaatac aaccatgacg aaacgaaagc caaagccgaa
    10021 tgggataaag tgacgagcaa acccaccagc ctgacgtttc tctactccga taacgatccg
    10081 aactgggagc ctattgctct ggcgacacaa tccagtctca acaagctggg catcaatgtg
    10141 aagctggaaa agctggcgaa cgccaccatg cgcgacagag tgggtaaagg tgattacgac
    10201 attgcgattg gcaactggag tccggatttt gccgacccgt atatgtttat gaattactgg
    10261 tttgagtcag acaaaaaagg tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc
    10321 gataagttac tgcgcaatgc gcttgcgacc accgaccaga cgcagcgtac ccgggactac
    10381 cagcaggcac agaaaatcgt cattgatgac gctgcttatg tgtacctgtt ccagaaaaac
    10441 taccaactgg cgatgaacaa agaggtgaaa ggctttgtgt tcaatcccat gctggaacag
    10501 gtcttcaata tcaataccat gagtaaataa cgtgaccttc tggagtattt tacgccaacg
    10561 ctgctggggg ctggtgctcg tggtggcggg cgtctgcgtg attacgttta ttatctcgca
    10621 tctgatccct ggcgatccgg cgcggttact ggcgggtgac cgcgccagcg atgctatcgt
    10681 ggaaaatatt cgccagcaac tgggactgga ccagccactg tatgtacagt tttaccgcta
    10741 cttcagcgac ctgtttcatg gtgacctggg aacatccatt cgtaccgggc gtccggtgct
    10801 ggaagagttg cgtatatttt tcccggcgac gctggaactg gctttttgtg ccctgctgct
    10861 ggcactcctg attggcatcc cgctgggcat actctctgca gtctggcgaa atcgctggct
    10921 ggatcatctg gtgcgaataa tggccattac cggaatctcc acacctgcgt tctggcttgg
    10981 cctgggcgtc attgtgctgt tttatggtca tctgcaaatt cttcccggcg gcggaaggct
    11041 tgatgactgg ctggatccac caacgcacgt taccggcttt tatctgctcg atgcgctgct
    11101 tgaaggcaac ggtgaagtct tcttcaatgc gttgcaacat ctcatcttac cggcattaac
    11161 gctggcgttc gttcacctgg gaattgtcgc tcgccagatc cgctcagcga tgctggaaca
    11221 attgagtgaa gactacattc gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt
    11281 tttatgttat gcgctaccca atgcgttgat cccatcgatt accgtattgg gtctggcgct
    11341 gggcgatttg ttgtatggcg cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg
    11401 tgcatgggta gtaacatcaa tacaggcgct cgacttcccg gcagtgatgg gctttgccgt
    11461 cgtggtttca tttgcttatg tgctggtcaa cctggtggtg gatttgctct atttgtggat
    11521 tgatccgcgt atcggacgtg gaggtggtga atgatgctaa gcgaggaaac gtccgccgta
    11581 cgcccacaaa aacaaacgcg atttaacggt gcaaaactgg tatggatgct gaaaggcagt
    11641 ccgctcaccg tgaccggcgc agtcatcatt gtattaatgc tattgatgat gattttttca
    11701 ccgtggcttg cgacgcatga tcccaacgcc attgatttaa ccgcccgcct tttgccgcct
    11761 tctgcggcgc actggtttgg caccgatgaa gtgggacgcg atctgttcag ccgcgtactg
    11821 gtcggcagtc agcaatcaat tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt
    11881 ggttcgctac tcggatgtct atccggtgtg cttggcggac gcgcagacgc catcatcatg
    11941 cgcatcatgg acattatgct gtcgattcct tcgttggtac tgacaatggc actggctgcc
    12001 gctctcgggc cgagtttatt taacgccatg ctggcgattg ctattgtgcg aattcccttt
    12061 tatgtgcgcc tggcgcgggg gcaagcatta gttgtacgcc agtataccta tgttcaggcg
    12121 gcgaaaacct ttggtgcgtc gcgttggcat ctgatcagct ggcatatttt acgtaactcc
    12181 ctgccgccgc tgatcgtgca ggcatcgctg gatatcggta gcgcgatttt aatggccgcc
    12241 acgttgggat ttattggcct gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta
    12301 gctaatggtc gcaactatgt actcgatcag tggtggtatt gcgcatttcc gggggcagcg
    12361 attttgctta ccgccgtcgg gtttaatctc tttggtgatg gtattcgcga tctgcttgac
    12421 ccgaaagcag gaggaaagca gtcatgaccc aacccgttct ggacattcaa cagctgcatt
    12481 tgagtttccc cggttttaac ggttatgttc acgtgctcaa caatgtgtcc ttgcagatta
    12541 accgcggtga aattgtcggt ctggtgggag aatccggctc aggtaaatca gtcaccgcaa
    12601 tgctgattat gcgtctgcta ccgacgggca gttattgcgt acatcggggg cagatttcac
    12661 tgctgggaga cgatgttctt aacgctcggg agaaacagct tcgtcagtgg cgcggcgcac
    12721 gagtggcgat gatctttcag gaaccgatga ccgccctcaa tccgacacgt cgaataggtc
    12781 ttcagatgat ggacgtgatc cgccatcatc aaccaataag tcgtcgggaa gccagagcta
    12841 aagcgattac cctgctggaa gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct
    12901 atccgtttga gctttcaggt ggtatgcgcc agcgggtaat gattgcgctg gcattctcct
    12961 gcgagccgca attgattatt gccgacgaac cgactacggc gctggacgtc acggtacagt
    13021 tgcaggtact gcgtttgctt aaacataaag ctcgcgccag tggaactgcg gtactgttca
    13081 tcagccatga tatggccgtg gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg
    13141 gaagcgtaat tgaaagcggc gtgacggcag acgttatcca tcatccccgg catccgtata
    13201 ccattggttt gctgcaatgc gcaccagaac atggagtacc acgccagcca ttacccgcca
    13261 ttccagggac ggtaccaaac ctcacccatt tgcctgacgg ctgcgctttt cgcgatcgtt
    13321 gctatgcggc aggtgcacag tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca
    13381 accagcgctg cgcctgttgg tatccgcagc aggaggtcat aagtatctga cacgttatta
    13441 acgttacgcg acgtccatat caatttcccg gcccgtaaaa gctggcttgg taaaactacg
    13501 gaacatgttc atgcgattaa tggtattgat ttacagatcc gccgtggtga aaccttaggg
    13561 atcgtcggtg agtcaggctg cggcaaaagc accctcgcac agcttttaat gggtatgctg
    13621 caaccgagcc acgggcaata catccgttca ggatcacaac gcattatgca gatggtgttt
    13681 caggacccgc tctcttcgct taatccgcgc ttatcggtgt ggcgcatcat cacagaaccg
    13741 ctctggatag ctaagcgtag tagtgaacaa cagcggcgag cgttggcaaa ggagttggct
    13801 gtgcaggtgg gtattcgtcc ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg
    13861 cagcggcaac gcatcgccat tgccattgcc agagcactct cttcgcagcc tgacgtgatt
    13921 gtgcttgatg agccaacctc ggcgctggat atctccgtgc aggcgcagat cctcaattta
    13981 ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg
    14041 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg
    14101 ggcgacgcgc agcaggtgct gacggcacct gcacatccat acacccgatt attgctggat
    14161 tccctccccg ccattgataa accgctggag gaagaatggg cattatgtaa aacggatctg
    14221 ccgggaaacc gcacgttgcc acaaggctgt tttttctacg aacgttgccc tctggcaacc
    14281 cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga gctccggtgc
    14341 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    14401 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    14461 aaaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    14521 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaatcg gcatccactt
    14581 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca
    14641 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    14701 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    14761 cgccactctg ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    14821 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    14881 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatacgg cgaaaatatt
    14941 acttctgcac ttaaattaaa ttcccacaat agataaccgg gaatatatat ctcaagagat
    15001 attgcatcaa tatcaatcaa ctcaccacaa taataataca tatcaatatc atggagttaa
    15061 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    15121 tgaatgtgct ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga
    15181 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    15241 gccattgagc aaggccttcg ggcagagcaa ggtgttgtca ctgaagatga cattctcatg
    15301 gagctgacca aatgggtgga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    15361 acgatcaatt atgtggtcag tggccagcac cctacgcttt aaggtgctat gcttgatcgg
    15421 caacctaatt taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact
    15481 agcaacagga ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac
    15541 tggaccataa aatttctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg
    15601 taaataaccc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    15661 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    15721 tggggccgac gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc
    15781 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    15841 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    15901 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
    15961 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    16021 tctacctgcg taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc
    16081 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    16141 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata
    16201 tggacgatat tctgcaaaac gtaccgaacc ataatatcaa agtgattgtg gtgactgacg
    16261 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    16321 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    16381 tggatgtcgg aacgaacaac caacaactgc ttaacgatcc gctgtatatg ggctggcgta
    16441 atccgcgtat cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga
    16501 aacaacgctg gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
    16561 cgttacttaa ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg
    16621 cggcggtaac agtcggcaca ctgatcgcag caagccgagc ggcaggtggt cagttaagcg
    16681 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    16741 tcgcccagac ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg
    16801 tcgatcgctt tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac
    16861 tggtgcagaa acgcgaaaac ctcagtgact gggataccga cagcgatgtg ctgtcactgc
    16921 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
    16981 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    17041 cgctgtctaa cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggaccg
    17101 aaggtaacgc gctggtcgcc acgggcagcc cgtttaatcc agtggtatgg aaagataaaa
    17161 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggggtta
    17221 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg
    17281 cacagtattc gccgctggtg ctgaacggcg aaggtctggt actaccggaa ctaaaagata
    17341 ttcagaaagt ctcccgtgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    17401 tggcggtgaa aacctctgcc gaagctttgc aacaagccat tgacgataat ttctggcaag
    17461 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    17521 cgggcaattt tcctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    17581 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    17641 gttgttacga aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat
    17701 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    17761 aatggcgatt ttggtgacaa aaccgacgta attctgggcc atgaaggcat cggtgtggtg
    17821 gcagaagtgg gtccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    17881 ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
    17941 tcagttaaaa atgccggata cagcgttgat ggcggaagag tgcatcgtgg tcgccgatta
    18001 cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg
    18061 ggtcaccacc tacaaagccg ttaagctgtc aaaaattcgt cccgggcagt ggattgctat
    18121 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcaaagaatg tctttaacgc
    18181 caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatgggtgc
    18241 agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg
    18301 tggcgctcac tctgcggtgg taacagcagt agctaaagct gcgtttaact cggcagttga
    18361 tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct
    18421 ggatatccca cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg
    18481 ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
    18541 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
    18601 tggccgtatg gtgattgatt tccgtcacta agaggtcttt gctgcgactc ccatgttcgg
    18661 gtcgcagcat cgcacactct ccaacatgaa atggctaaat ggattattgt tgcgtaggaa
    18721 ggaacaataa tgatagtgtt cccagagaag gaacgctact gagagtaaag acactaatga
    18781 aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac gaacttaatg
    18841 tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca agccgattgc
    18901 tgaccggaaa agcagctttg acgccagaaa tggcaattaa actttccgtg gtgatcggca
    18961 gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag gcagaaaaaa
    19021 cggtggatgt gtcgcgatta cgccgtctgg ttacgcaata aaaaatggca ccttataaag
    19081 cgccatttga aactgatgac aaacgcaaca ttgcctgatg cgctacgctt atcaggccta
    19141 cacgttcact tcaatctatt gtatttgaag ggttttcatg ccggataagg cattcacgac
    19201 gcatccggga tgaacaaagc ccacgttgtc tacaatatga aaatggcgcc ttttacagcg
    19261 ccattttcag cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt
    19321 ctgccttctc gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca
    19381 ccttcccttc gatcatccct ttaatcgacc ctttcaccca gaatgccata tacatatgga
    19441 tcaggatggc gtggatcagg atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa
    19501 caacctgcat cgggaagtac tgcgcaaagt acggacgcca gataatcacc ccggtcacca
    19561 gcagcacgaa aatcatgctc atgatcgacc agaacatcat cttttgcccg gcgttgtact
    19621 taccaacatc cgccactttg tgctcattgc cttttaatac ttcgacaatg ttcaacagcc
    19681 acggaatatc tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca
    19741 gtgcgacgaa aatcgcaatg ccgaagaacg ggtgcaaaat gcgtcccatc tgcggcgtac
    19801 cgaaggtttg cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca
    19861 ccaggaagaa gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca
    19921 caatcatttt cgacttactc atgatgatcc tcctcgtcat cgtccacttc cttattcggg
    19981 ccaataccga tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc
    20041 agcggtttca acgcgccttt ccacaggctt accgaggtgt cgatcttcgg atctttcggc
    20101 agaccgtaat acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca
    20161 ccgacccctt tcgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc
    20221 acgcgctctt ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc
    20281 ggacaggttt tcacacaagc cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg
    20341 catttatata cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca
    20401 atgcagtaac cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac
    20461 tgaatgattg caccagcaga cgggcacgcc ttcaggcagc cgggatcttc acagtgcata
    20521 cagccgtctt tacggatcag ccactccagc ttgccgttct gttcggtttc gctaaagcgc
    20581 atcaccgtcc aggacttggc gctcagatcg gcggggttat cgtaaacccc gacgcagtgc
    20641 cccacttcat cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg
    20701 atacaggtgg aaacgtcgat aagttttgcg acttctgctt tgtaatcacg cacctgagaa
    20761 ggcggcgtga tggagttagt tgcggacctt ttgataatat cctgcgtttc catagccatc
    20821 tgttcgcccc cttacgcctt ctcgatgttg actaagaacg ctttatattc cggcgtttgc
    20881 gagtttgcat caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca
    20941 ccctcaaagc cccagtggat tggaataccc accgtttcaa cctgctgacc gtttacattc
    21001 agcggtttca gacggcgcgt taccaccgcc accgcgcgga taaagccacg cttgcttgag
    21061 acagtgacac gatcgccatt attaatgcct ttcgccgccg ccagcgtttc gctgatttcc
    21121 acaaactgtt ccggctgagc aattgcgttg agcaatgcgt gcttggtcca ggtgtggaag
    21181 tgctcggtca gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc
    21241 agcgcgtctt gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcagcggg
    21301 ttagtgccca gcggcgtttc aatcggctcg tagtgttccg ggaacggacc ttccgccatt
    21361 ttgttgatgg caaacaggcg tcccatccct tccggctgca tgataaacgg cccggttggc
    21421 gtacccggtg cggcattgcc gaagtccgga atatcgttac ccgtccactt gctgccgttc
    21481 cactggatca gcatccgttt cggatcccac ggtttaccat tgatatccgc cgaagcacgg
    21541 ttgtagagca cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga
    21601 ccggatgggt cggagttatc gcggttagcc atctggttgc cctgctctgt ccagctaccg
    21661 gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc aaagctactc
    21721 agcaactgac ctttcttcgc aatcagcacg ccattagcgt catagagatc ttccagcgca
    21781 tagccgttat tctctttcgc cacttcgtca gattgcggtt cgtgcggctg cttgtagttc
    21841 cagctcatct tcatcagcgg ttctacgcct ttaccacctt cggcctggta cagctcgcgc
    21901 agatgatggt agatacccgc cagaatttcg ccgtcgttac gcgcttcgcc cggcgcgtcc
    21961 tgacctttcc agtgccactg caaccagcga ccggagttag cgatagaacc atcttcttcg
    22021 gcaaagcagg tcgaaggcag acggaatact tcagtctgaa tagacgccgg atcgacatcg
    22081 ttcgactcgc cgtggttctg ccagaaggta gaggtttcag tcaccagcgg atcgataacc
    22141 accatgtact tcagcttgct caggcagctc accactttgt ttttgtccgg gaaggacgca
    22201 accgggttaa agccctggca gaagtaaccg gtgactttgc cttcgtccat catgttgaaa
    22261 tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc ccagttgttc
    22321 tctttctgcg cggcatcgcc atagaaagat ttcatcaggc taacgaagaa cttcggatag
    22381 ttgctccagt agttcacctg atcagccagc gtcgctttcg gcgtgttcgc ttccagatac
    22441 gactgcaaat caacctgttt ttctgacgga agcgtcagat aacctggcag gctggtagag
    22501 agcagaccta agtcagtcag cccctgaatg ttggagtgac cacgcaatgc gttcacgccg
    22561 ccaccggcca tacccatgtt accgagcagc aactggatca tcgccatagt acggatgttc
    22621 tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc
    22681 ggtgcgctgg tggaggccag cacttcacac actttcagga agtcggcttt tggcgtaccg
    22741 cagatgtttt caacgacgtc cggcgtgtag cgggaaacgt gcgctttcag caggttccac
    22801 acacagcgcg gatgagtcag tgtttcatcg cgttttgcat agccgttttc atcgaactga
    22861 tagttccagg acgacttatc atactggcgt ttttcagcgt cgtagccgct gaacaggccg
    22921 tcttcgaaag caaaatcatc acgcaccagc aggctggcgt tggtgtaatg tttaacgtat
    22981 tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg
    23041 tccgtaccgg aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacga
    23101 ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa
    23161 ccgacgggat gcgcttcagc agcgttaccg cccattacca ttacgacgtt agcgtttttg
    23221 atatccaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag acttgctacc
    23281 gttggtccgt gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca
    23341 aatttctggg tcagcatccc ggtttcgttg ctggcaccgg aggcacacag cataccggta
    23401 gaaagccaac ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg
    23461 tcagctttca tcagcttcgc aatacgggag aatgcttctt cccagctaat gcgctgccat
    23521 ttgtcagaac ctggcgcacg atattccggg tagcgcagac ggttttcgct gttgacgtaa
    23581 tccagcaaac cggccccttt tgggcacagc gcaccacggc ttaccggatg atccgggtca
    23641 ccttcaatgt gataaatcgc ttctctggcg tttttcgcgc catcacccag gctatacatc
    23701 aatagcccgc aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat
    23761 aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc
    23821 gttgttccag ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt
    23881 gctttcctct tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc
    23941 tttttgcgcg aaatcaaaaa gaacacgtgt atgccacaaa ataatccgcc tgcgcaaatt
    24001 aacctacctc aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg
    24061 ctataaaatt cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgac
    24121 aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga
    24181 ttcgcggtgt cagcgagggg ctcggtccgg tcggcggcgc agctgctatc tattcattaa
    24241 gcgggctgct gttaatcttc acggttggat ttccgcgtat tcggcagatt ccgaaaggct
    24301 atttactcgc cgggagtctg ttattcgtca gctatgaaat ctgtctggcg ctttccttag
    24361 ggtatgcggc gacccgtcat caggcgattg aagtgggtat ggtgaactat ctgtggccca
    24421 gcctgacaat tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac
    24481 caggattatt attagccctg gtcggcgtct gctgggtgtt aggcggtgag aatgggttac
    24541 attatgatga aatcatcaat aatatcacca ccagcccatt gagttatttc ctggcgttta
    24601 ttggtgcgtt tatctgggca gcctattgca cagtaacgaa taaatacgca cgcggattta
    24661 acggaattac cgtttttgtc ctgctaacgg gagcatgtct gtggatttac tattttctca
    24721 cgccacaacc agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat
    24781 ttaccttagg atttgcttat gctgcatgga atgtcggtat attgcatggc aatgtcacca
    24841 taatggcggt aggctcgtat tttacgcctg tactttcctc agcgcttgca gccgtgctgc
    24901 tcagcgcccc gctgtcgttc tcgttctggc aaggcgcgct aatggtctgc ggcggttccc
    24961 tgctctgctg gctggcgaca cgtcgtggtt aaaataattg ccgggtctac ggcccggcag
    25021 ttaatcagat aaattccgac aatattaaaa aataaatttc aaataccagt aattcacctt
    25081 atatctcatt tcgacttcga tcacatatcg ataaataata ttatttcatc tatttttctc
    25141 attgttttta atcaccattc tattttaaaa acagtatgtc atttataaat gacgccgttt
    25201 aaaattcgtt tagaaaatac gtttaacgat aattaaaagg attctgttaa atgaaattaa
    25261 aaatagttgc ggtggttgta actggtttgt tagctgcgaa cgtagcacac gctgccgaag
    25321 tctataacaa ggatggtaat aaactcgacc tttatggcaa ggttaccgct ctacgttatt
    25381 ttactgatga taagcgtgac gatggtgata aaacttatgc ccgtctcggc tttaaaggag
    25441 aaacgcaaat caatgatcaa atgattggtt ttggtcactg ggaatatgat tttaaaggct
    25501 ataacgatga agccaacggc tcgcgcggca acaagacccg tctggcctat gctggtttaa
    25561 aaattagtga atttggctct ctggactatg gtcgtaacta cggtgtcggc tacgacattg
    25621 gttcatggac cgatatgttg ccagaatttg gtggcgatac ctggagtcag aaagatgtct
    25681 tcatgacata ccgtaccacc ggtctagcaa cctatcgcaa ctacgatttc tttggcttaa
    25741 ttgaagggct gaactttgcc gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc
    25801 atctttatgg tgctgactac acgcgtgcca acggtgacgg tttcggtatc tcctcaactt
    25861 atgtttatga tggctttggt atcggtgcgg tatataccaa atccgatcgg acaaatgcgc
    25921 aggaaagagc cgctgctaat cctctcaatg cctccggtaa gaatgcagaa ctatgggcca
    25981 caggtataaa gtatgatgcc aacaacatct actttgcagc taattacgct gaaacattaa
    26041 acatgaccac ctatggggat ggttatatct ctaacaaagc acaaagtttt gaagtggtgg
    26101 cgcaatatca attcgacttc ggcttgcgcc catcactcgc ttacctgaaa tcgaaaggca
    26161 gagatctggg ccgctacggc gaccaggaca tgattgagta tatcgacgtt ggtgcgacgt
    26221 atttcttcaa caaaaatatg tcgacctatg ttgattataa aatcaacctg attgatgaaa
    26281 gcgactttac ccgtgccgta gatattcgga ccgataacat cgtcgctacg ggcattacct
    26341 atcagttcta acgtcctctc ctgaccagcc cttctctggg ctggtttttg atttaagtgc
    26401 tgctgtatga ctccttaagt aaaaatgagc aatttttgat atttctggtc attcacctca
    26461 attaatatta ttccatatcc tccggggaca tcttactata atatttattg atgagtgatt
    26521 atgaccaata ttaatacagc ttgtgtaaaa aataatgcca gttatcaact taataacgca
    26581 ttacctaaca aagaaaccat ctccagcaat ttttgtgaac gactagaaca atggggtaat
    26641 aaatcggtta ataatggtga agaaagagca attgccgtgg agcgaattaa agaagcttac
    26701 aattcgaaca tggcatctct tgatttgtca tatcttgatt tgagtgaatt accgcctatc
    26761 ccctcgacag ttaatacgtt aaatctggaa aacaattgtc taacttgtct tgacttagct
    26821 gacaatgcca gtctcgttaa tatcaacctc agcgttaata aaattaacac gataaccttt
    26881 ccaaaagaat caaaactgga aaacatttat attgatcata acaatctgga aagtttagat
    26941 ttaaaaaatc agcattcatt ggttaatctg gaagcgcaaa ataacaaact gacaaaaatt
    27001 aatatttctg atagttataa actgaaattt cttaatcttg attataacaa gctagcatcg
    27061 ctggatgtct cccggcaaga atctctgatt gagttaagtg cccatcacaa tatgatcact
    27121 gaccttatat tacacaatca tcccaggatg aaaacaatca ctttaaacga caaccatatt
    27181 gcacatttaa acgccaaaaa cactacaaaa ctggaatatt taaacttaag caataacaat
    27241 ttactgccaa ccaatgacat tgatcaacta atatcatcaa aacatctttg gcatgtatta
    27301 gttaacggca tcaacaatga tccacttgca caaatgcagt actggactgc agtaagaaat
    27361 ataattgatg acactaatga agtgaccatt gatttatcag gacttaattt aaccactcaa
    27421 ccaccagggc tgcaaaactt cacatctatc aatcttgata ataaccaact cacacatttt
    27481 gatgcaacca actacgatag actcgtaaag ctaagtctga atagtaatgc tcttgagtca
    27541 ataaattttc ctcaaggcag aaatgtaagt attacacata tatctatgaa taataatgct
    27601 ctcagaaata ttgatataga taggctttca tcagttactt attttagtgc ggcacata
//
LOCUS       DAQSWQ010000049        27470 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.049, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000049 DAQSWQ010000000
VERSION     DAQSWQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.049"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..81)
                     /locus_tag="R8G18_002214"
     CDS             complement(<1..81)
                     /locus_tag="R8G18_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
                     /protein_id="HEE9795899.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="R8G18_002215"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="R8G18_002215"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HEE9795900.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="R8G18_002216"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="R8G18_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HEE9795901.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="R8G18_002217"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="R8G18_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HEE9795902.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="R8G18_002218"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="R8G18_002218"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HEE9795903.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="R8G18_002219"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="R8G18_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HEE9795904.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="R8G18_002220"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="R8G18_002220"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEE9795905.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="R8G18_002221"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="R8G18_002221"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEE9795906.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="R8G18_002222"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="R8G18_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="R8G18_002223"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="R8G18_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HEE9795907.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="R8G18_002224"
     CDS             complement(12387..12626)
                     /locus_tag="R8G18_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795908.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="R8G18_002225"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="R8G18_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HEE9795909.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="R8G18_002226"
     CDS             13003..13290
                     /locus_tag="R8G18_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HEE9795910.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13960..14103
                     /locus_tag="R8G18_002227"
     CDS             13960..14103
                     /locus_tag="R8G18_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HEE9795911.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14116..14325
                     /gene="cspE"
                     /locus_tag="R8G18_002228"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14116..14325
                     /gene="cspE"
                     /locus_tag="R8G18_002228"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HEE9795912.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14491..15300
                     /gene="rlmA"
                     /locus_tag="R8G18_002229"
     CDS             14491..15300
                     /gene="rlmA"
                     /locus_tag="R8G18_002229"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HEE9795913.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="R8G18_002230"
     CDS             complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="R8G18_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HEE9795914.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16292..16750)
                     /locus_tag="R8G18_002231"
     CDS             complement(16292..16750)
                     /locus_tag="R8G18_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HEE9795915.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="R8G18_002232"
     CDS             complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="R8G18_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HEE9795916.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17669..18469)
                     /gene="manY"
                     /locus_tag="R8G18_002233"
     CDS             complement(17669..18469)
                     /gene="manY"
                     /locus_tag="R8G18_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HEE9795917.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18532..19503)
                     /gene="manX"
                     /locus_tag="R8G18_002234"
     CDS             complement(18532..19503)
                     /gene="manX"
                     /locus_tag="R8G18_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEE9795918.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19966..21522
                     /gene="yoaE"
                     /locus_tag="R8G18_002235"
     CDS             19966..21522
                     /gene="yoaE"
                     /locus_tag="R8G18_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HEE9795919.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(21526..23124)
                     /locus_tag="R8G18_002236"
     CDS             complement(21526..23124)
                     /locus_tag="R8G18_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEE9795920.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="R8G18_002237"
     CDS             complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="R8G18_002237"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEE9795921.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24803..25381)
                     /locus_tag="R8G18_002238"
     CDS             complement(24803..25381)
                     /locus_tag="R8G18_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEE9795922.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="R8G18_002239"
     CDS             complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="R8G18_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HEE9795923.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26820..26999
                     /locus_tag="R8G18_002240"
     CDS             26820..26999
                     /locus_tag="R8G18_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HEE9795924.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27119..27442)
                     /locus_tag="R8G18_002241"
     CDS             complement(27119..27442)
                     /locus_tag="R8G18_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HEE9795925.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7067 a         6772 c         6941 g         6690 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    13441 aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt gattaagcgt
    13501 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca tcaatttttc
    13561 ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat tgatagccgt
    13621 taaccgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcaaaaaat
    13681 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
    13741 gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca
    13801 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
    13861 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
    13921 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
    13981 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
    14041 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
    14101 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
    14161 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct
    14221 atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
    14281 gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
    14341 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
    14401 atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
    14461 tgcggccttt agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag
    14521 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
    14581 aaagaagggt atgtcaatct gctgtccgtt cagcataaac ggtctcgtga tccgggcgac
    14641 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
    14701 cgtgatgcaa ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg
    14761 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc
    14821 accacgtttg gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg
    14881 caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac
    14941 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc
    15001 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
    15061 atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta
    15121 cagcagagtg cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
    15181 ttgcagatga cgccgtttac ctggcgtgcg aagccagaag tctggcaaac actggcagca
    15241 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
    15301 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga
    15361 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt
    15421 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
    15481 aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
    15541 agtagccaga aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa
    15601 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc
    15661 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg
    15721 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
    15781 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
    15841 agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca
    15901 tcgcccccag caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca
    15961 ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc
    16021 cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc
    16081 ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt
    16141 atttcattgc tttattgata acgattttca tttggattta tatatcaatg aaacgttaaa
    16201 ataaataaca aataagggaa gaggtaatat agcctaagct atgtctgatt attaaaaata
    16261 cactaaacat agctttggct aaattcataa cttattgagt tgaaacgaga agcttataaa
    16321 ttttttccag atcgtcgata tttcgaacgc ggattaacag ccgacgctgt tctaattgca
    16381 tcaccaacac gccatcttcc gacaagttca tcgctttgat tcggctatat tcaatccaga
    16441 cattggcgaa gaaaaaacct ttttgtttaa agatgatctt cggaacgcgg atccagaata
    16501 tataaaaacc catcagagcc agtgcgctta ataaccatgt cgttattaac gcaccatgat
    16561 tcgtgacgtt gttatagata agaatgacaa tcaatccgac gaagataacg ctatcgatgc
    16621 gaccacgccg gagcaaagga attgccagca gggtggggcc gttacggcgg ggcatgatga
    16681 actgatcgta gatcgcgaag gccaggagtg cggcgatgaa aagaatcagt accaggtccg
    16741 tgattgtcat taatcctcca gataaaaaaa cggggccaaa aggccccggt agtgtacaac
    16801 agtcttacag tcccagcagg ccgcaagcgt aaccagcgat accgatgacg aagaagccaa
    16861 cgatgatcca cagcgggtta acttttttgc gcagtagcca catacaagca aaggtcagca
    16921 gcagtggtac caggcctggc attaactggt ccagaatagt ctggacagta gtaacgtgtt
    16981 ctttgcccgt ctggtcagta atgcgagaga caaccagcgg gatgttgaca tgtgtccact
    17041 tgttaaccaa tgcccccatg acaaacaggc cgaggataga cgccccttcc gtcagttttt
    17101 gcaggaagcc accacccata tctttaacga tatcgatacc tttggagtaa ccatacgcta
    17161 cgccgtagta acgggttgcc agacgcacca ggttaaacag gatgaagaac agcagcggac
    17221 ctaacaggct gccgctcatc gcgataccgg cacccagtgc tgcaaatacc ggacgtacgg
    17281 ttccccagaa gatcgggtcg cctacaccag ccagcggccc catcaaaccg actttgatac
    17341 cgttgatagc accgtcgtcg atctctgcgc cattagcacg ctgttcttcc agcgccaggg
    17401 ttacgccgag aatcggcgca gccacgaacg gctgggtgtt aaagaactcc aggtgacggc
    17461 gaatagcttg tttacgagct tcgttgttct cagggtagag gcgacgaatt gccggtacca
    17521 tagagaagca gaaacccagt gcctgcatac gttcgaagtt ccatgaaccc tggaagaggt
    17581 tagaacgcag gaagacgcca cgaatatcac tttgagtgag ttttttctcg gtggtagttt
    17641 gagttgtatc aaccatttcg ctcacctgtt agtccagttc gttatcgaga tcgttgttac
    17701 cagctgcctg agcaggcgca ccggctacgc ggttgtattt cgggctaagt tggatgtaga
    17761 gcactgccat aacagtacca atcacaccca gagcaaccag gttaaagttg gtgaatgctg
    17821 cggttacgaa gccgaggtag aagaacggca tcaggtagcc agcacgcatc atgttgataa
    17881 ccatcgcata accaaccacc acgatcatgc caccggcgat attcagacca ttggtcacca
    17941 cttccggaat cgcattcagc atgttctgta cttcgctggt accaacagac agcgcaacga
    18001 tgacggccgg aatagccaca cgcattgctt gcaggaacag agaagaaacg tggatccagg
    18061 aaatcgctgt caggttgccg ttatcagcag ccttatccgc agcgtgctgg aaagcaacgg
    18121 taatagtacg aacgatgatg gtcagtacct ggcccgcagc ggccagaggg attgccagtg
    18181 cgatacctgc accaatgctc tgatgacctg cgataaccag aatggtagaa atgatagaag
    18241 ccagagcggc gtcaggtgca actgcagcac cgatgttcat ccagcccagc gcgatcattt
    18301 ccagcgtacc accgataata ataccggttt tcatatcccc aagaacgata cccaccaggg
    18361 tacacgcgat tagcggacgg tgaaactgaa attcatcgag gattgatccc atacctgcga
    18421 tacaggctac gataaatacc agcacaattt gaagagtggt aatctccatt gtacttctcc
    18481 tgtttacgta agacttaagt gtgaaaactg agtgataatc aacacaatac gttacttatc
    18541 gattttgctg atcagatcca tcattttcag tttcggatcg gtggaaacct tacggacttc
    18601 cagctcaata ccgcgcgcat tcagtttctt gaacgcctca atatcttttt catcaaccga
    18661 aaccgcgtta ttcacctggg ttttaccctg acggaatgcc ataccaccga cgttaacaga
    18721 ggtgattttc acgccgcctt caacgagacg ctctacatct gttgggttgg taaataacag
    18781 cattacgcgt tcgccagcat atttcgggtt gttgtagacg cgaatcattt tggcaacatc
    18841 aactacgtgt gctgttacgc ccggaggtgc aacctgggtg agcagtgtct tacgaacggt
    18901 atccgcagcc acttcatcac taacaacaat aatacgggag acattggttt ctttggtcca
    18961 gcgggtggcg acctgaccgt gaatcagacg gtcgtcgata cgcgcaaggc caataaccat
    19021 gtagtcgttt ggccccattg gttttgccgg agttggagcc gcttttggtg ctgcggcagc
    19081 gggtgctggc gcggcttttt caaccggttt ggctttcagt gctttcacgc cttcacggcc
    19141 tgtttctact gccagcgcaa ccagttcatc aaagcttggg tcatcatcac gagccattaa
    19201 cgtttccacg agcattggaa tgttaacgcc tgcaatgact tcataatgct ctttgtcgac
    19261 gacaatgcgg ctggcagcat tgaacgggct gcctccccat gtatcaacga gaaacagcac
    19321 gcctttagtg gtgtcgagtt ttgccaactg agcgttgtac ttttcaatca gcgtttcggc
    19381 attttcacct ggaacgaaat cgatccagcc gacgttttcc tgctcgccta acagcatttc
    19441 tgccgtttta agcaactgct ctgcagccca accatgtgtg cctataacaa tagcaatggt
    19501 cacttgctac ctcctttatt atcgttaaca cctcaacgtg ccagatgtat ttttgaatcg
    19561 tcgtccacaa tcgaatcgat tcagataagg gctacaacca aaaacccctg cgtttcgcga
    19621 tttattttag atatcgaaaa aattatttta tgtgatgaag atccgtaatt taactttcga
    19681 ttagcagaaa tttcgaaagg taaaatatcc ttgtcacatt cgtttgcaaa ggaaggtaaa
    19741 tctttgccaa atcagaggcg tctctgatat gtttaactcc cgtttaacaa ccatggagta
    19801 tagggcagta gcccgcagta tggatcgtca ccgacgtcat ttcagcatca ggccttttaa
    19861 cgcctgcctt tctggcactc tatgccgcac ctttcgtttg cattttgtcg ttacgcctgc
    19921 attatttctg gcgtcgaata gctattcctt aagcaggagc ttgtcatgga attcttaatg
    19981 gacccctcaa tttgggcggg gctactcacg cttgttgttc tcgaaattgt gctgggtatc
    20041 gataacctgg tcttcatcgc cattcttgct gacaaactgc cgccaaaaca acgcgataaa
    20101 gcgcgtttgc tggggttatc actggcgctg attatgcgtc tggggctgct gtcgctgatt
    20161 tcatggatgg tcacgctgac caaaccgcta tttaccgtca tggatttctc cttctccgga
    20221 cgcgacctga ttatgttgtt cggggggata ttcttgctgt tcaaagcaac aaccgaactg
    20281 catgaacggc tggaaaaccg cgatcatgat tccggccacg gtaaaggcta cgccagtttc
    20341 tgggtggtcg tcacacagat tgtcatcctt gacgccgtct tctcgttgga tgcggtaatt
    20401 actgcagtag ggatggttaa ccatctgccg gtgatgatgg cggcggtagt gattgcgatg
    20461 gcggttatgt tgctggcatc caaaccgctg acgcgattcg ttaaccagca ccccacggtg
    20521 gtggtgctct gtctgagctt cctgttaatg attggtctga gtctggtggc agaaggtttc
    20581 ggtttccaca ttccgaaagg ttacctgtat gccgcgattg gcttctcgat catcatcgaa
    20641 gtgtttaacc agattgcgcg tcgcaacttt attcgccacc agtcgacttt gccgctgcga
    20701 gcgcgtactg ccgatgccat cctgcgtttg atgggcggga aacgtcaggc caatgttcag
    20761 cacgatgccg ataacccgat gccgatgccg atcccggaag gtgcatttgc cgaagaagaa
    20821 cgttacatga ttaacggcgt actgacgctt gcgtcgcgtt ctctgcgcgg gatcatgacg
    20881 ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat ccgcgagcaa
    20941 ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga tgaaatcatc
    21001 ggtattgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga tgtggcggcg
    21061 attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat caacctgttg
    21121 ggcgtgctgc gtcgtgctcg cgggagcttt gttatcgtga ccaacgagtt tggtgtggta
    21181 caaggtctgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt cccggacgct
    21241 gacgaaacgc cggaaatcat tactgatggt gacggctggc tggtaaaagg cggtacagat
    21301 ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga tgatatcgcg
    21361 acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg cgatgtgatt
    21421 gatgtagggc cactgcatat caccatcatt gaagccaatg attatcgtgt tgatctggtt
    21481 cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcattaac gtaacggcat
    21541 aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg ccagccattt
    21601 gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa tatgcacgcc
    21661 atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca ccagttcaat
    21721 attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa ccgcgtcaga
    21781 acctatagct gcggtaaatg acttatcaat tttcagcatg tcaggacgta atgtttcaag
    21841 ccaggaaaat gaactgttgc cggtgccgaa gtcatcaatc gccagtttga cgtttttacg
    21901 atgtagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt cggtgatttc
    21961 gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat cttttatcag
    22021 cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact gactactcat
    22081 cgggaaaaca tggcgctgac gaatggtttc tgccatcaca tagcgggtca gtggcacaat
    22141 taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc cctgacgcgg
    22201 attgttccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg cattaagcaa
    22261 aggctgacag aacaattcga actcatgttg cgccaggccc agattgattt cgcgggaaaa
    22321 gctcatccgg taagcggtcg ccagccaggc gatgtagccc accagtaaac ttagcagcac
    22381 cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc ccggaccatt
    22441 aacgttaatg gtaaaaggaa agcgttgcga agaaacctga tagttttcat tgtcttcagg
    22501 ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt ccaccgtcag
    22561 gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca gtaagtcgat
    22621 gttaatcatc tccatgaccc cagcattgct actgcccgct gcaggcgtcc attgaatcaa
    22681 aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg gttgcggtgc
    22741 aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa tgctggagca
    22801 atagagtgtt ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt ttgccgcctg
    22861 tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa gcaagacttc
    22921 gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt gattggcgaa
    22981 ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc tcagggtcga
    23041 agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc gataggtttt
    23101 aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg acaaacgcct
    23161 gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg ggtataaatt
    23221 cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact ttgattgcca
    23281 gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccagtt tcgtacatcg
    23341 tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga gccatccgtg
    23401 cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc acctgaacct
    23461 gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt tccgccgcca
    23521 cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca gccattgaac
    23581 aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag gcgttcattt
    23641 tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg tccgggctga
    23701 ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg caggcaccgt
    23761 tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact gccagcgcaa
    23821 acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg ctggaaacta
    23881 acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc agtacacctt
    23941 cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc gcgaaatact
    24001 cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga ccagacagcg
    24061 aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac ggatacggca
    24121 cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata aaaccgccgc
    24181 cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg tggatttgca
    24241 taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg tcgcgcggga
    24301 aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctcttct acgtcgcgaa
    24361 taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca agacccataa
    24421 taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca taaacgtcca
    24481 cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg acgaactgtt
    24541 tacctgcctt cataggccct acggtatggg aagatgaggg accaatcccc accttaaaca
    24601 tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc cagtaacgaa
    24661 ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat tcacatgaat
    24721 taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa gtagtgtttt
    24781 gtagatgtaa agataagtat agtcagggtt tcacaccaat ttgcagcgcc agctcacgaa
    24841 ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc catacccgat
    24901 gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt aatgcctggg
    24961 cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac ggcagatcgg
    25021 gcgggataat gccgaccact ggggttacct gatagccagt tacgctatcg acgggcggca
    25081 gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca gcttcgcgca
    25141 gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg aatgccactt
    25201 gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc ggttgcggtc
    25261 gacggacgat ggggattaac acggcagcct gacgatgatt tagggtttcc cggttaattt
    25321 gcgggcgcaa aagttgaaag cgcgataaaa aatcatcaag catcaggcta cggtattcca
    25381 cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt tcctgatatt
    25441 ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat atttgtccgt
    25501 taatggcagt cagagtgcgg atagtaatgc tggtatccat gttgccgcaa aagctcaaat
    25561 agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg ataatttcca
    25621 tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg cgcagcagat
    25681 cgctggcgtg taactgttct ggtagttgtg ccgttatggt gctgaccaga tgatgcacgg
    25741 cagggaaggg ttccaccacg aacagctctg gtacttttac cgaacctgct acggcaacac
    25801 gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga tctttcgctg
    25861 agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg cgtggtagcg
    25921 tgcctttaat agggcgggtc tggatttcac tattatcaca aagaataaac cgctctggcg
    25981 aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc gcgcggttgg
    26041 cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca tgaaaacgct
    26101 gggcgagatt tacctgatag caatcaccgc tgtgcagata ttcctgtacc tggcgaaatt
    26161 tttcgccgta ctgctcgcgg gtcatattgg attgccagtc ggaagtaagc gtgaaatctt
    26221 cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca tcattatgac
    26281 tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg tagataccca
    26341 ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac tcaaaacggc
    26401 ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa tcttcgttat
    26461 gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc gggtcatcag
    26521 tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa gtggttaaag
    26581 tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg gcatagccgg
    26641 agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat tcagcggcgt
    26701 cctgacgcca gggtaaagta atcacagcgg gagataacgt cttcatcagt cctgactcta
    26761 ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag gagttaacaa
    26821 tgtttgcagg tttaccttca ctcacccatg aacagcagca aaaagctgtc gagcggatcc
    26881 aggaactgat ggcacaaggg atgagcagcg gccaggcaat cgcgttggtg gcagaagagc
    26941 tgcgtgccaa ccattccggc gagcgaatcg tggcgcgttt tgaggatgaa gacgagtaac
    27001 acactaatcc gtattgattt cgttatacaa gccgatgatt atttgattgt gagaaaaagg
    27061 acgcttttta tatgtgatta aaagcgtcct gatccattta tgctgatgta aattttactc
    27121 agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga tttttaatca
    27181 cagagcgttc acctgactcc attttttttg cccatccaac cattttttcc gtaaacccag
    27241 ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgca ggtactccca
    27301 gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa cttggtgttg
    27361 gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca gcaggcatat
    27421 tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
//
LOCUS       DAQSWQ010000050        27104 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.050, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000050 DAQSWQ010000000
VERSION     DAQSWQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.050"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..921
                     /locus_tag="R8G18_002242"
     CDS             <1..921
                     /locus_tag="R8G18_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9795926.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="R8G18_002243"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="R8G18_002243"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEE9795927.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="R8G18_002244"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="R8G18_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HEE9795928.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTTGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="R8G18_002245"
     CDS             3882..4277
                     /locus_tag="R8G18_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEE9795929.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="R8G18_002246"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="R8G18_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HEE9795930.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="yraP"
                     /locus_tag="R8G18_002247"
     CDS             4897..5472
                     /gene="yraP"
                     /locus_tag="R8G18_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HEE9795931.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="R8G18_002248"
     CDS             complement(5586..6626)
                     /locus_tag="R8G18_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEE9795932.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="R8G18_002249"
     CDS             complement(6699..7334)
                     /locus_tag="R8G18_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEE9795933.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="R8G18_002250"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="R8G18_002250"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HEE9795934.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="R8G18_002251"
     CDS             complement(7960..8403)
                     /locus_tag="R8G18_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HEE9795935.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="R8G18_002252"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="R8G18_002252"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HEE9795936.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="R8G18_002253"
     CDS             complement(8743..9246)
                     /locus_tag="R8G18_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEE9795937.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="R8G18_002254"
     CDS             complement(9240..9764)
                     /locus_tag="R8G18_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEE9795938.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="R8G18_002255"
     CDS             9973..10968
                     /locus_tag="R8G18_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEE9795939.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="R8G18_002256"
     CDS             10977..11855
                     /locus_tag="R8G18_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEE9795940.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="R8G18_002257"
     CDS             12061..13068
                     /locus_tag="R8G18_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HEE9795941.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="R8G18_002258"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="R8G18_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEE9795942.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="R8G18_002259"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="R8G18_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HEE9795943.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="R8G18_002260"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="R8G18_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HEE9795944.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="R8G18_002261"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="R8G18_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HEE9795945.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="R8G18_002262"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="R8G18_002262"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEE9795946.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="R8G18_002263"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="R8G18_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEE9795947.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="R8G18_002264"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="R8G18_002264"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEE9795948.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="R8G18_002265"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="R8G18_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEE9795949.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="R8G18_002266"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="R8G18_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEE9795950.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="R8G18_002267"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="R8G18_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEE9795951.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="R8G18_002268"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="R8G18_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEE9795952.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="R8G18_002269"
     tRNA            complement(26826..26902)
                     /locus_tag="R8G18_002269"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6421 a         7516 c         6810 g         6357 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccacagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcagtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatggcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca agctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc ctta
//
LOCUS       DAQSWQ010000051        25774 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.051, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000051 DAQSWQ010000000
VERSION     DAQSWQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.051"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="R8G18_002270"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="R8G18_002270"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HEE9795953.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="R8G18_002271"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="R8G18_002271"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEE9795954.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="R8G18_002272"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="R8G18_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HEE9795955.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="R8G18_002273"
     CDS             2440..3063
                     /locus_tag="R8G18_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HEE9795956.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="R8G18_002274"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="R8G18_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEE9795957.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /locus_tag="R8G18_002275"
     CDS             complement(3447..3512)
                     /locus_tag="R8G18_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795958.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="R8G18_002276"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="R8G18_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HEE9795959.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="R8G18_002277"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="R8G18_002277"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEE9795960.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="R8G18_002278"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="R8G18_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HEE9795961.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="R8G18_002279"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="R8G18_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HEE9795962.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="R8G18_002280"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="R8G18_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HEE9795963.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="R8G18_002281"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="R8G18_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HEE9795964.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="R8G18_002282"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="R8G18_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HEE9795965.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="R8G18_002283"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="R8G18_002283"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEE9795966.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="R8G18_002284"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="R8G18_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEE9795967.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="R8G18_002285"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="R8G18_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEE9795968.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="R8G18_002286"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="R8G18_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEE9795969.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19898..20824
                     /gene="gluQRS"
                     /locus_tag="R8G18_002287"
     CDS             19898..20824
                     /gene="gluQRS"
                     /locus_tag="R8G18_002287"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEE9795970.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20863..22281
                     /gene="pcnB"
                     /locus_tag="R8G18_002288"
     CDS             20863..22281
                     /gene="pcnB"
                     /locus_tag="R8G18_002288"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEE9795971.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="R8G18_002289"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="R8G18_002289"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEE9795972.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="R8G18_002290"
     CDS             23127..23711
                     /locus_tag="R8G18_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9795973.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="R8G18_002291"
     CDS             23816..24556
                     /locus_tag="R8G18_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HEE9795974.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25774
                     /gene="htrE"
                     /locus_tag="R8G18_002292"
                     /pseudo
     CDS             24591..25774
                     /gene="htrE"
                     /locus_tag="R8G18_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6330 a         6986 c         6416 g         6042 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc tgtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc gccacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgcg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctca agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgcgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ataactatct aacg
//
LOCUS       DAQSWQ010000052        25636 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.052, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000052 DAQSWQ010000000
VERSION     DAQSWQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.052"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(64..795)
                     /gene="rlmB"
                     /locus_tag="R8G18_002293"
     CDS             complement(64..795)
                     /gene="rlmB"
                     /locus_tag="R8G18_002293"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEE9795975.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(975..3416)
                     /gene="rnr"
                     /locus_tag="R8G18_002294"
     CDS             complement(975..3416)
                     /gene="rnr"
                     /locus_tag="R8G18_002294"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEE9795976.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3455..3880)
                     /gene="nsrR"
                     /locus_tag="R8G18_002295"
     CDS             complement(3455..3880)
                     /gene="nsrR"
                     /locus_tag="R8G18_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HEE9795977.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4085..5383)
                     /gene="purA"
                     /locus_tag="R8G18_002296"
     CDS             complement(4085..5383)
                     /gene="purA"
                     /locus_tag="R8G18_002296"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEE9795978.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5487..5684)
                     /locus_tag="R8G18_002297"
     CDS             complement(5487..5684)
                     /locus_tag="R8G18_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEE9795979.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5766..6770)
                     /gene="hflC"
                     /locus_tag="R8G18_002298"
     CDS             complement(5766..6770)
                     /gene="hflC"
                     /locus_tag="R8G18_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEE9795980.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6773..8032)
                     /gene="hflK"
                     /locus_tag="R8G18_002299"
     CDS             complement(6773..8032)
                     /gene="hflK"
                     /locus_tag="R8G18_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEE9795981.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8118..9398)
                     /gene="hflX"
                     /locus_tag="R8G18_002300"
     CDS             complement(8118..9398)
                     /gene="hflX"
                     /locus_tag="R8G18_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEE9795982.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9475..9783)
                     /gene="hfq"
                     /locus_tag="R8G18_002301"
     CDS             complement(9475..9783)
                     /gene="hfq"
                     /locus_tag="R8G18_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEE9795983.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9869..10819)
                     /gene="miaA"
                     /locus_tag="R8G18_002302"
     CDS             complement(9869..10819)
                     /gene="miaA"
                     /locus_tag="R8G18_002302"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEE9795984.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10812..12659)
                     /gene="mutL"
                     /locus_tag="R8G18_002303"
     CDS             complement(10812..12659)
                     /gene="mutL"
                     /locus_tag="R8G18_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEE9795985.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12669..14006)
                     /gene="amiB"
                     /locus_tag="R8G18_002304"
     CDS             complement(12669..14006)
                     /gene="amiB"
                     /locus_tag="R8G18_002304"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HEE9795986.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14025..14486)
                     /gene="tsaE"
                     /locus_tag="R8G18_002305"
     CDS             complement(14025..14486)
                     /gene="tsaE"
                     /locus_tag="R8G18_002305"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEE9795987.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14458..16005)
                     /gene="nnr"
                     /locus_tag="R8G18_002306"
     CDS             complement(14458..16005)
                     /gene="nnr"
                     /locus_tag="R8G18_002306"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HEE9795988.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16004..17143
                     /gene="queG"
                     /locus_tag="R8G18_002307"
     CDS             16004..17143
                     /gene="queG"
                     /locus_tag="R8G18_002307"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEE9795989.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17126..17179)
                     /locus_tag="R8G18_002308"
     CDS             complement(17126..17179)
                     /locus_tag="R8G18_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9795990.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17413..17488)
                     /locus_tag="R8G18_002309"
     tRNA            complement(17413..17488)
                     /locus_tag="R8G18_002309"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17453..17455),aa:Gly,seq:gcc)
     gene            complement(17524..17599)
                     /locus_tag="R8G18_002310"
     tRNA            complement(17524..17599)
                     /locus_tag="R8G18_002310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17564..17566),aa:Gly,seq:gcc)
     gene            complement(17757..17832)
                     /locus_tag="R8G18_002311"
     tRNA            complement(17757..17832)
                     /locus_tag="R8G18_002311"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17797..17799),aa:Gly,seq:gcc)
     gene            complement(18043..18588)
                     /gene="orn"
                     /locus_tag="R8G18_002312"
     CDS             complement(18043..18588)
                     /gene="orn"
                     /locus_tag="R8G18_002312"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEE9795991.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18683..19735
                     /gene="rsgA"
                     /locus_tag="R8G18_002313"
     CDS             18683..19735
                     /gene="rsgA"
                     /locus_tag="R8G18_002313"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HEE9795992.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19832..20800
                     /gene="psd"
                     /locus_tag="R8G18_002314"
     CDS             19832..20800
                     /gene="psd"
                     /locus_tag="R8G18_002314"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEE9795993.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20822..24145
                     /gene="mscM"
                     /locus_tag="R8G18_002315"
     CDS             20822..24145
                     /gene="mscM"
                     /locus_tag="R8G18_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HEE9795994.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24296..>25636)
                     /gene="yjeM"
                     /locus_tag="R8G18_002316"
     CDS             complement(24296..>25636)
                     /gene="yjeM"
                     /locus_tag="R8G18_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HEE9795995.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5928 a         7193 c         6501 g         6014 t
ORIGIN      
        1 atcttaccag ctatagacaa aaaaaccatc caaatctgga tggcttttca taattcagac
       61 gaactagctg cgctgacgaa ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt
      121 cagggaagaa acacttcctg ccattgggat gctgatcaac tcatcgcaat gttcacgggt
      181 caggcgacgc ataccttcac cttccgcgcc catcaccagc gccaagcgtc cggtcatttt
      241 gctctgatag agtgtatgat ccgcctcgcc tgccgtaccg acgatccaga tattctcttc
      301 ctgcaacata cgcatggtgc gcgcaaggtt agtcacccga atcagtggaa cgctttctgc
      361 cgcgccgcag gctacttttt tcgccgtagc gttgagctgt gcggagcgat ctttcggcac
      421 aatcaccgcg tgaacgccag cagcgtccgc gctacgcagg cacgcgccga ggttgtgcgg
      481 atcggttaca ccgtcgagga tcagcaggaa cggttgatcg agcgaagcga tcagatccgg
      541 cagatcgttt tcctgatact gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac
      601 ggcaccgtcg cttttctcgt cgagatattg gcggtttgcc aactggataa ccacgccctg
      661 agactcaagg gcgtgaatca gcggtaacag acgtttatct tcacggcctt ttaaaataaa
      721 gacttcctga aaacgttcag gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta
      781 aatcatttcg ctcattaatg tactcgttgt ttacgttttg cctgatgcac tacgtttatc
      841 cggcctacat gatctctgca atatattgaa tttgcgtgat tatgtaggcc ggataaggcg
      901 ttcacgccgc atccggcatg aacaaagcgc attttgtcag caatctaacc ctcttctttt
      961 aaagagggta ttgatcactc tgccactttt ttcttcgccg cacgcttcgc tttggtcgct
     1021 gcggctattt tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc tttcttcgcc
     1081 gctttcggct tcgttttttt ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt
     1141 ttaccgacct gacgacgctt gccgcctttt ttgcctgcat cgcctttttt cgctttctcg
     1201 cgcgccgttt taccgacgtt gcgcggtgcg cgttcactgg agatcagact aaagtcgatt
     1261 ttgcgctcgt ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa
     1321 gtctggccgc tggattcccc catcaggcgt tgccctacct ggtcaaagcg atagtagtca
     1381 ttgtccagcg aagagacatg gaccagacca tcaatgaaca agtcgtccag acggacgaag
     1441 aagccaaagc cagtgacgct ggaaattacg cctttaaaga cgttacctac ctggtcgagc
     1501 atgaagtcac acttcagcca gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc
     1561 gccatcgaac agtgctgacc cagttgcaac atctcttcca tcgaataatg gtagccgccg
     1621 gtttcagtgg tgttgccctg atgcccctgc tctttcgcca gcagatactt aatggcgcgg
     1681 tgcagcgtca ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat
     1741 gccaggccaa agtgaccacg gttttctgga tcgtaaatcg cctgtttcat cgagcgcagc
     1801 agcatggttt gcagcatttc tgcatcagga cggtcggcaa ccgactccag cagttccgcg
     1861 tagtcacgcg gttccggctt gttaccaccc ggcagctcca gccccagctc cgccagcact
     1921 gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt
     1981 tctttcgctt tctcaacgaa acgcgccgcc gagatattcg ccagaatcat gcactcttca
     2041 attaatttgt gcgcgtcgtt acgctgggtc tgttcgatac gttcaatacg gcgttcagcg
     2101 ttgaaaatga acttcgcttc ttcgctctca aatgagatcc caccgcgttc ttcacgggct
     2161 ttatccagca ctttatagag gttatgcaac tcttcgagat gcttaaccag cggggcgtac
     2221 tgctcgcgca gatcctgatc gccctgcaga atatgccaga ctttggtgta ggtcagacgc
     2281 gcgtgagagc tcatcaccgc ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa
     2341 accgtcatct cgcacaccat acacaggcgg tctacctgcg ggttgagcga acacaggccg
     2401 ttagagagca cttccggcag catcgggata acctgcgaag ggaagtacac cgacgtgcca
     2461 cggttacgcg cttctctgtc cagcggcgtt ggcggacgca catagtagct gacgtcggca
     2521 atcgcgaccc ataaacgcca gccgccgccg cgttttttct cgcagtaaac tgcatcgtca
     2581 aagtcacggg cgtcttcgcc atcaatggtg accagcggta aatcgcgcaa atcgacacgg
     2641 cccgcttttg cttcttccgg cacttcttct ttcagccccg caacctgttg ctcaacagcc
     2701 tgcggccaga tatacggaat ttcatgggta cgcagagcga tatcaaccgc catgccggtg
     2761 cccatattgt cgcccagcac ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga
     2821 cgctgagtca gttcgactac gaccacaaag cccatccgcg cgcccatgat ctgatcgggc
     2881 gggattaaga tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct
     2941 tcggtaaagt agcgaccaac aatctggctg gtttttggca ccagtacgcg gacaatacgc
     3001 gcttcacgac gacctttacg gtcagcaccc agcggctgcg ccagcacctg atcgccatga
     3061 atacaggttt tcatctgctc gctggagaga tacaaatcat ctttacgccc ttcaacccgc
     3121 agaaagccgt agccatcacg gtggccaata acggtacctt tcaccaggtc gaggcgttcc
     3181 ggcagcgcat agcactgacg acgagtgaag accagttgac catcgcgctc catcgcgcgc
     3241 aggcggcgac gcaggccttc aagctgctct tcgccttcaa tgtgcagttc taccgccagc
     3301 tcatcacggc tggccggttt ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta
     3361 gggatgggat tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg tgacatctcg
     3421 gttcctccgt tgtcatctct gatgaagatt ttcgtcactc caccagcaat aatttataaa
     3481 gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac
     3541 tttgcacggc cttagaaagc gcctgtttca accgacaggc aggtgtaatg tggcaaaact
     3601 cactgctgca attcaccagc gataagggct ccagctcgcg caccacatca ccaatacgta
     3661 tcgcactcgc cggtttaccc aggcgaatgc cgccattttt tccacgaaca gcagtcacgt
     3721 agccggcacg actaagttga ttgattattt tgaccatatg attacgggag acgccgtaga
     3781 cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg caatgacgcc atgtagatca
     3841 gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac atcaacctca aaagggaaat
     3901 cgggaaaaaa tacatttata ttgatgataa accagccaca agctgtgtcg ctaatttatt
     3961 tcagttcggg gaaggaaaaa agcgaggatt taacactgtg tcggatagcg taatggctta
     4021 accgacacag caatgtatca cactgttagc ccggcaggca aaatatctgc caggcgtacc
     4081 agaattacgc gtcgaacgga tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag
     4141 agatgatatc gatcggcaca ccggtcagct cttcaatacg cttgatgtag ttcagcgccg
     4201 cctgcggcag gccgctacga tctttcacgc cgaaagtgga ttcagaccag cccggcatgg
     4261 tttcgtaaat cggctctaca cctttccagt cgtcagctgc cagcggagtg gtagccactt
     4321 cgcgaccatc cggcatacgg taagccacgc agagtttaac ctctttcagg ccatccagaa
     4381 cgtccagctt ggtcaggcag aagccagaca gggagttcag ctgtaccgca cgacgaacgg
     4441 caacggtgtc cagccagccg gtacgacgac gacgacccgt agttgcgccg aattcgttac
     4501 cctgcttgca gaggaactcg ccagtttcat caaacagttc ggtcgggaac ggacctgcac
     4561 ctacacgagt tgagtaagct ttgaggatac ccagaacata atcaacataa cgcgggccca
     4621 ggccggaacc ggtcgccacg ccaccagcag tggtgttgga agaagttacg tacggataag
     4681 taccgtggtc gatatccagc agcgtaccct gcgcaccttc aaacatgacg aaatcgccac
     4741 gctgacgcgc ctggtcgagc aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg
     4801 caacagccat cgtatcatcc agaactttct ggtaatcaac cgcttcagct ttgtagtagt
     4861 taaccaactg gaagttgtga tattccatca cttctttcag tttttcagcg aaggtttctt
     4921 tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgctac tttatcttca taagcaggcc
     4981 cgataccacg accggtggtg ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat
     5041 ccagcgcaac gtgataatca aggatcagcg gacatgcttc agacagcagc agacgctcac
     5101 gaacggggat gccacggtct tccagttctt tcatctcttt catcagcgcg gccggagaca
     5161 gcacaacacc gttaccgatg atgctggtta cattctcgcg gagaatacct gatggaataa
     5221 gatggagaac ggttttttca ccgttgatta cgagagtatg gcctgcgttg tgaccgccct
     5281 ggtagcgtac aacatattta gcccgttcag tcagaagatc gacgatctta cctttacctt
     5341 cgtcacccca ttgggtgccc agtacgacga cgttgttacc catttttcaa aatcaccgtt
     5401 tgcttaaaaa tggattctac catcgctttt tcagagttac agcacttttt gcactcaaaa
     5461 atgacctcga tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat gtagtagacc
     5521 acaacgcccg caaccacaag tccaccgcca aaacgacgta aaatattatc gggcaaattg
     5581 gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc
     5641 agtaccaaaa ccagggcaag cgccagccag attgtcgaat tcattcatta tccttataga
     5701 aaaagaaaac caccgacatc cctgaggatg cggtggcttt attgacctgt accgcagtcg
     5761 ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg
     5821 ctcatgacca tcacgtcctg attgccagag aagctgttct cataagcacg caggctacgg
     5881 atgaatgcgt agaagtccgg atctttactg aatgcatcag caaacagttt ggctgcttcg
     5941 gcatcgcctt caccacgcat gatgcggccc tgacgctcag cttctgccag cgttctggtc
     6001 acttcatagt cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg tgaacggtga
     6061 cgacgcgcta ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc agacacttcg
     6121 gtcggcaggt tgatctgctt gatacgcaca tcgacaactt caatacccag cgccgccata
     6181 ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg ctctgccgct
     6241 tcggcaatgg cgttatctgc cgccggggta gtaacttcat cttctgtacc cgcagaaccg
     6301 gagttcagcg cgtcacgtac ttcgagggtc agacgaccac gggaatcggt gacgatgtct
     6361 ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag agaacttacg tttcaacagc
     6421 acttccgctt gcgaaatgtc gccaccaccc gttgccaggt agtaacggct gaaatcgctg
     6481 atgcgccatt tgatgtaaga atcgacgatc aggtctttct tctctttggt cacaaagcgg
     6541 tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac
     6601 ggtatcttga aatgcagacc cggctcataa accagaggtt tgttgtcatc gtcacgcagt
     6661 accttaccaa aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa gacagacatg
     6721 taaagcacta ccagcacgat gatgataatc gcgataactg acttacgcat cgttattccc
     6781 cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg ttggtccata atatcgccct
     6841 gactggtgga cgacgtgttg cttgctccac tggttgtgga ggaagaggct ggcggcagac
     6901 gcagcagatt gctggcaccg ttatcgctct tcgccgcagg ggcgttaccg cctttcagca
     6961 tctggtctaa cggcagaacc atcaggttgc cacctttatc gttaaccagc actttgcggg
     7021 tgttacccaa cactttttcc atcgtctcga tatacagacg ctcgcgagta atttccggcg
     7081 cggctttata ttccggcaga agtttagcaa agcgcgccac ttcaccctga gcttccagga
     7141 tggtctgggc cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac
     7201 gcggctgaac ttcgttggta tacgcttctg cttcacgaat gtattgctgt tcgttttcac
     7261 gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc cggcggacga gctgcctgga
     7321 agttgacgtc cagcagagtg atacccatgt catacggacg gatagtctct tccagttcgc
     7381 gctgagtatc gctacgaatc acggtacgac cttccgtcag aatgcggtcc atggtgtatt
     7441 taccgataac tccacgcagg gcgctgtcgg tagcctgacg caggctgtca tccgggctgg
     7501 tcacgctata cagatatttt tccggattgg tgacgcggta ctgcacgttc atctcaacgc
     7561 gcactacgtt ctcgtccgac gtcagcatca caccagaagc ggccagttca cgcacggctt
     7621 ccacgttcac cggtttgact tcgtcgataa acgtcggttt ccagttcaga cccggctcaa
     7681 ccagatggct gaatttacca aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat
     7741 agaaaccact ggccgcccag ataatgacaa tcgctgccgc tgcgatggta acgacacgac
     7801 cgccaagctg cgggcgcggg ccttgcgatg aactgccacc gccagatccg gtgcctttac
     7861 cgccgcccag accaccgagc tttttgctca gtttgcggaa gatatcatct aaatcaggtg
     7921 gcccttgatc gcgaccgcct ttgtttccat ttccctcaga gttgccgcca ggtttgctgc
     7981 ttccccacgg gtcgcggtct tgtccgttat taccgggctg attccacgcc atgtttgtgc
     8041 tccatatttg ttatgcggtg atccctgttg tctttcgacg ccagaggata tgactccacg
     8101 cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg tttacagagg
     8161 cgacgccagt caacgatcgg catacgaact tgcagactta cgctgccgtc ctcctccatc
     8221 cactcttttt ctattgcctg aagctgataa aaacgacttc tcagacgtcc ttcctgcggc
     8281 ggcaaacgca atgtatgctg cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa
     8341 agctgtggta tccccgctcc ggtctgtgcg gaaagccaga cacggatcgg tttgttctct
     8401 tcgtcccgat caatacgcgg ttcgaaatct tccagcatat cgatcttgtt catcaccagc
     8461 agggttggga tctcgtgagc gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt
     8521 tcttgtacac gtacatccgc cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc
     8581 tcttgtaacg tggctttaaa tgctgccacc agatcgtgcg gcaggtggcg aataaaccct
     8641 acggtatctg caagtacggt ttcaccgaca tccgcgacgt caatacgccg caacgtcggg
     8701 tcgagggtgg caaacaactg gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa
     8761 agggtagatt taccggcgtt ggtatatccc accagcgaaa cagtaggaac gtcggctttg
     8821 atacgcgatt gccgcccctg ctcacgctgc ttttcaactc tttctaggcg cgactgtatc
     8881 tgcacgatgc gattacgcaa caaacgacgg tcggtttcga gctgggtttc acccggacca
     8941 cgcaaaccta tcccgccttt ctgtctttca aggtgggtcc agccacgcac caggcgcgta
     9001 gccagatggc gcagctgcgc cagctcaacc tgcaacttac cctcatgggt acgcgcacgt
     9061 tgggcgaaaa tatctaaaat aaggccggtg cggtcgataa cacgacactc gcacaaacgc
     9121 tccaggttac gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc
     9181 gtcgctttga cagcttccgc aatttcaact gctttacctt cacctacaaa atactttggg
     9241 tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc
     9301 agagattcaa actcctggag gtcttccata tctttgtctt gcgtaaaata gatgtgtacc
     9361 agtaccgcct gctcaccagc atcataacgg tcaaacaagc gtataaccct ctaaatagat
     9421 cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa aacagcccga aaccttattc
     9481 ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat gatggtagtt
     9541 actgctggta ccgccaccgg cgttgttact gtgatgagaa accgggcgag acgggacaac
     9601 agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa
     9661 ctgatcaaaa gactcgattt gcccttgcag cttaatacca ttcaccaaat aaatagaaac
     9721 tggaacacgt tcccgacgca gtgcgttcag gaacggatct tgtaaagatt gccccttagc
     9781 cattctctct tttccttata tgcttatttg tactttgaac ctttcgattc tgaaaaaatt
     9841 gcgcacgata cgtctcaatt gtacacattc agcctgcgat agcaccaaca acctgtaata
     9901 cttcgtcacg cgcctgttct ggtttttcac tgtcaagcca gtgaacccct tcccaaccac
     9961 gcagccaggt tatctgccgc ttcgccaact gtctcgtggc gcaaacacct cgataaacca
    10021 tttcatcgta tgagatttcg ccttcaaggt aagaccacat ctggcgataa cccacgcaac
    10081 gaatggaagg caagtccgta tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt
    10141 caaaacctga agccaacatc tgatgaaaac gctgctcaat gcgttgatgg agcagttcac
    10201 ggctcgccgg ggcgatggcg aactgatgca cctgatacgg tagagcgtct cctgacgttt
    10261 gcgtcagttc cgttaaagtt ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc
    10321 tttgtggatc atttggatga atccttgctg ccgcaaccgg atctacctcc tgaagttgac
    10381 gatgcaatga ctcccaacct tgctctgccg cctgttgctc aattctggcc cgcacttccg
    10441 ggtctgccga cggtagcggc gacaaccctt ccagcaatgc cttgaaatac aacatcgtac
    10501 cgcccactaa cagtgggatc cgccccgccg cggtgatatc ggccatttcc gccagcgcat
    10561 cgcggcgaaa atcagcagcc gagtaagcct gcgacggatc gcgaatatcc agcaatcggt
    10621 gcggcgcggc gagtaactct tcagcgttcg gcttcgccgt cccgatatcc atccctttgt
    10681 aaataagggc agaatcaacg cttatcaact ctactggtaa aattttacgc agctcaatgg
    10741 ctaacgccgt tttaccggag gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg
    10801 ccttactgat atcactcatc tttcagggct tttatcgccg gatgtaaatc aacagattgt
    10861 aacagaccac ccggcggcgt tttcacaagt tgcggacata accgttccac gtccgccagc
    10921 agggttatgg cctgtgccat tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc
    10981 cactgcgcaa tattgccagg ttcgaatacg gactgcttcg ccaggtagcc tatcagttca
    11041 ggaatcaaga tttgtaaatt ttgttggcgt aagggtaaag gcacggccct gatggtcaca
    11101 tgctgtgcat ctgactggaa atcaataccc aattccgcca gggcagactg cgctttttct
    11161 aatgccgatt tttcttcgcc agaaactttt agccgcaacg gaataagcag cggctgagcg
    11221 caaactggcg cttcacccgg cgtcagttgt acctgacgca gccaacgttc tgccactggc
    11281 aaggctaaaa gtgaaatgtt gccgtcgcgc tccagcaacg cacagtcgga atggacgata
    11341 gtcagtaccc gaccaaaact ctgactgttc gccgcaagtg caggttcctg cggttccggc
    11401 gcttttaatt tttgcatcgg cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc
    11461 tgtttctggt agcctggctg cgcattcggc cagggggcag ccggacgact gcctgatgct
    11521 ggcgcaggag tgtagcgcgg agctaccggc tcacgagctg ccggttctgc aaagtgattg
    11581 cgccccgccg ccacgcggtt ttccggaatg gaacgcggtg caggttgggg ttcatcgtcc
    11641 agcggtagcg gcgtttccag ttgctgttgt agcacgctca gcacgccctg atagataaaa
    11701 tcatgcacca gacgcgactg atggaaacgc acttcgtgtt tggcggggtg cacgttgacg
    11761 tccacctgat gcggatcgat ctccagatac agcacaaatg ccggttgctg atcggccccc
    11821 agtttgtctt cgcaggcctg gcggatcgcg tgattgatca ggcgatcgcg catcatgcga
    11881 ccgttcacgt agcaatactg aatttctgcc agtgcgggcg tggtgtgatt tggatcggcc
    11941 acccagccgc gtagcgtgag atcgccgtgt tgccattcaa tcgccagcgc ttgttcaaga
    12001 aaagcggtgc cgcaaatcgc gcctaagcgc cgttcttttt gcccgccttc cggcactgcg
    12061 cggtactgac gcacaatttt accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc
    12121 agcgcaatgc ggcggatgat ctcatcaatg tggttaaatt cggttttctc ggtgcgcagg
    12181 aatttgcgcc gcgccggggt gttgtagaac agatccagca cctccagcgt cgtccccaca
    12241 ggatgcgccg ccggttttac cgtcacgttc atatcgcgcc cttcggcata ggcctgccag
    12301 gcttcctgct gttctgcggt gcgtgaagtg agcgtcaggc gggaaaccga actgatactc
    12361 gccagcgcct caccgcgaaa gcccaggctg ataatggctt cgagatcgtc cagagaggcg
    12421 attttactgg tggcatgacg agccagcgcc agcgccagct catctttttt gataccgcag
    12481 ccgttatcac gaatgcggat aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc
    12541 gtcgcacctg catcgaggct gttttccacc agttctttga ctaccgacgc aggtcgctcg
    12601 accacctcac ctgcggcaat ctggttcgcc agttgtggcg gtaagacctg aattggcatc
    12661 aatcgtcctt agtttggcag cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc
    12721 gttgccccct gcggcgcaga ttgcatcgga tgcgcaagga aataattgcg caggcctttg
    12781 taaatggctt ctgccagctg ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg
    12841 ttgttgctga taaaaccggt ttcgaccagt actgacggga tatccggcga acgcagaacg
    12901 ccgagactcg cgtgttctgg tcggcgttta tgtatttcgc caatgcgttg caactgactg
    12961 atcatactgg tcgctacatc ataccctacc cgctgggaat gaccgaactg taaatccagc
    13021 accgcctggc ttaaataggg gtcagactga ctgttcgcca gcacatcgcc cgccccaccc
    13081 agcagctccg actgtttctc gtgctgctcc agccagctgg ccatttcgct gttggcgcga
    13141 cggttagaga gcacccatac ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg
    13201 tgaatcgaca cgaggaaatt ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc
    13261 gagataaagt aatccccgtc acgggttaaa acgcctttaa acatcggatc gtcattgagc
    13321 aaagtgcgca atttacgcgc gatggcgatg gtgacatttt tctcccgcgt accaccgggg
    13381 ccgatagcgc cagggtcctg accgccgtgt ccggcatcaa tagcgatgat aattttatcg
    13441 ccagtgttag ccgtcgcgcg cgctgccgga cgcgttaccg tattactgct gataacaccc
    13501 gtagtgcggt tactttccgt tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg
    13561 acaaccgcag gcgtttcaac gcgtttcgca accacgggcg gtggaggagg cggtggcggc
    13621 acatcggcgt taatcgtaaa gacgacagtg taattgctgc cattctgccg cttcaccgct
    13681 tcggttttac cgttttcggt aagatcgacc accagccgta gcgtttgtgc atctttaggc
    13741 gttccagagc gaatcgcctt caccagatta ttgccgctga acaacaacgg cagtccctga
    13801 atcacgcccg tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca
    13861 taatcaggat cgccaataaa actcaacgtt atccgcgcct gttgattacc gttagaaacc
    13921 tgaatatcag agagcgtcgc ggcacccacc ggcgtgcaca gcagcagcag cgtcgctacc
    13981 aaccaatttc tgatgcgata catcatcccg ccacctttca aaggttaacc ggctaaacgc
    14041 gccagcaaca attcacccgc agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc
    14101 tgataatcaa tgtgtatttc gacatccggg tcaggaagaa cacctgtacc ttgttgtggc
    14161 cactccacca ggcagatggc atcgttggca aaataatcgc ggatccccat aaactccagc
    14221 tcctcgggat cggcaaggcg gtacaaatca aagtgataaa ccattaaatt gtcgagcgta
    14281 tagggttcga ccagcgtata agtggggctt ttgacattac cctgatgacc cagagcttgt
    14341 aaaaaacccc ggctaaaggt ggttttacct gcgcctaaat cgccatacag atagattacg
    14401 gttgcgccat cgcaggcttt cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca
    14461 gggagcggaa ttactcgatt catcatggtt tttatcagtc acttccgggt taacaatacg
    14521 ctgtagcgtg gaaaagagat cggttgccag catcccgcgc gttccaaaac gcgccgccag
    14581 tacgtcagct gccgcaccgt gcgcgacaca gcccgcacag gcggcatcat acggcgacag
    14641 tttttgccca agcaatgcgc caataatacc agagagcaca tcgcccatgc cgccgctcgc
    14701 catgcctgca tttccggcat caataatgcc taaagcgtca ggatgggcgg cgaccacggt
    14761 tccggcacct ttcagcaccg ctacgccgcc ataacgttgt accagacgtt tggcgcaatg
    14821 taagcggtca ctttcaattt cagcgacgga acagcctaac aaccgtgcgg cctcgccagg
    14881 atgcggcgtg atcacgcgat tgtgacgctt atcgggattg attgccagca ggttcaatgc
    14941 atcggcatcc cacaacatcg gtttgcgaaa attctcaact ttttgcagtg cttttttccc
    15001 ccactcttgc tggcccagac cgggaccaat caccaccaca tcggcccatt ccaggctttc
    15061 ggtaagagag tccatcgtca gttcatgcac catcaattcc ggtcgtgcag tcagcagcgg
    15121 cgcaatgttt tcactgcggg tcagtactcg gactaaacca gcaccagcac gcagcgccgc
    15181 ttcccccgtc atacgaatag cccccgccgt gccgtgatcg ccaccgataa tcaccagccg
    15241 cccgtgatcg cctttatgcg aagtcgggcg acgcggtttc agccagtgag aaagttgttc
    15301 tgccgaaaac cgctgaattt tcgtctcctg acctgccagc cagctatcca gccccagtga
    15361 gtcaaaatgc agttgtcctg taacatcccg cgcttttcca gtgagcaagc ctggtttcag
    15421 cgcaataaaa gtgatggtgt gatcggcgtt gatcactgcg cctggcgtag cgccggtttc
    15481 agccagcagg ccggaaggga tatcaaccgc cacaatcggc gcaggatggg aattagcgtg
    15541 gtcgattaac tggctaatgg attcgcgggg cgcttgctgc aagccggtac cgagcagcgc
    15601 atcaacaatc agatctaccg attcgggcca gacaatattc gaagcatgga tctcgccacc
    15661 cgcgtttaac catgcttcgc gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc
    15721 ctgggccaac aacgtgacct caatgccgac cgctttggcc agtcgcgcga ccacgtagcc
    15781 atcgccgccg ttattaccat gaccgcacag caccagccag tggcgggcgt caggatacgc
    15841 cgaacgacac acctggaatg cggcctcgcc agcgcgaagc atcagctcat agagtgtgag
    15901 ccccagcaca tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt accagacggt
    15961 gtgtggtata cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga
    16021 tctcaatcag ttagcgcaaa aaattaaaca gtgggggctg gaactgggct ttcagcaggt
    16081 aggtattacc gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa
    16141 acaataccac ggcgaaatgg actggatggc acgtcacggt atgctgcgcg ctcgccccca
    16201 tgagttatta ccaggtacgc tgcgtgtaat cagcgtgcgg atgaattacc ttcctgctaa
    16261 cgccgcattt gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct
    16321 gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa aagctgggcg agatgattca
    16381 gcaacattgt gtttcgctga attttagacc gtttgtcgat tctgcgccta ttctcgagcg
    16441 cccgttagct gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg
    16501 cgaggccggt tctttcttct ttttaggcga attgctggtc gatattccgc tgcccgtgga
    16561 tcaaccagtc gaggaaggat gcggtaaatg catagcctgt atgacgattt gcccgaccgg
    16621 tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga
    16681 acttgaaggg gcgatcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg
    16741 cgatgactgc cagcttatct gcccgtggaa tcgctattcg caactcacta cagaagacga
    16801 tttcagcccg cgtaagccgc tacacgcaca ggaactcatt gagttatttg cctggagcga
    16861 agagaagttt ttaaaagtca cggaaggatc ggcgattcgc cgtataggtc acctgcgctg
    16921 gctgcgtaat atcgccgtgg cattaggcaa tgcgccctgg gatgaaacga ttctggcggc
    16981 gctggagagt cgcaaaggtg agcacccact tcttgatgag cacatagcgt gggcgattgc
    17041 gcagcaaatc gagagacgaa atgcgtgcat agtcgaagtg caattgccga aaaaacagcg
    17101 tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg
    17161 aataaaaata aaaacgcatt gcaattcaag acgcataaaa tcgtcaagtg atcgactcga
    17221 caaaacagaa tcttgatttt taacttattt atcaataggt taaaaaaata ccattcaccg
    17281 tgcgaagttt ctttgaaatt aagattacag cctgaggatg tggataactc tgtttacaag
    17341 agtttgtcag agacaacaaa aaccagcaat aacgcatcgc gtcgctgtgg atattttatt
    17401 gagagaagaa tttggagcgg gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt
    17461 cgtgctctac caactgagct attcccgctt gggtggtctg tgccttacag cactttcaaa
    17521 ttttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta
    17581 ccaactgagc tattcccgct taatcttcgt ctttcgaacc gctactgcct tggctgcctt
    17641 cactcacctt agtcacatac ttttgtaagt tcccaaggat tcgttcagtt gccgccttgt
    17701 cgcaattcga aatactgcga ttgggtggtc tgtgctttgc agcacttttc aaattttgga
    17761 gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg
    17821 agctattccc gcgtactact taattttgct ttcgtaattt tcgcatttct gcgtcgttac
    17881 gggaggcgca ttatacgaga aatctttccc tctgcaaccc cccaaaaaag ttttttttgc
    17941 gtttttatct caagtgctga aattatcagc aagacgttca atttagcgtc aaaatcacgc
    18001 cgccgcaagt ggattccggc gcttacgtga ccaggaaaaa ttttacagct tgataaaatg
    18061 ctcgcggtag taagccagct ccgccaccga ttcacggata tcatccattg cctgatgcgt
    18121 cccctgcttg gtaaaaccat ccagaatttc cggcttccag cggcgcgcca gctctttcag
    18181 ggtgctgaca tcgagataac ggtagtggaa gtaggcttcc agctccggca tgtatttaaa
    18241 caggaaacga cggtcctgtc cgatgctgtt accgcaaatc ggcgattttc ccgcaggcac
    18301 ccactgtttt aagaattcga gcgttgccag ttcagcttcc cgatcgccca tcgtgctcgc
    18361 tttcacgcgc tctaccagcc cgctggcggt atgggtgcgc acgttccagt catccatcag
    18421 cgccagctgt tcatcggact gatgtactgc aatggtcggc ccttctgcca gaatattcag
    18481 attggcatcg gtcaccagcg tggcaatctc aataatgcga tcgcgctcgg gatccagacc
    18541 ggtcatctca agatcgatcc aaatcaggtt gttttcattg gcactcatgc tattttccac
    18601 ccattcacag tgactatatt gatccacaat tgcgtgtatc atagatgttt tgcccatcag
    18661 gggcgaccag gagtcagtac gattgagtaa aaataaactc tccaaaggcc agcagcgccg
    18721 cgtgaacgcc aatcaccagc gtcgtcttaa aacgtctaag gagaagcccg actacgacga
    18781 caatctgttt ggcgagcctg atgaaggtat cgtcatcagc cgctttggta tgcacgctga
    18841 tgtggaatcc gccgatggcg acgttcaccg ctgcaatatt cgccgtacca tccgttcgct
    18901 ggtaaccggc gaccgcgtag tctggcgtcc gggtaaaccg gcggcggaag gcgtaaatgt
    18961 caaagggatc gtggaagcgg tgcatgagcg tacctcggtg ttgacgcgtc cggatttcta
    19021 cgacggcgta aaacctattg ccgccaacat cgaccagatt gtcattgtct ccgccatttt
    19081 gccggagctg tcgctcaata ttatcgaccg ttacctggtg gcctgcgaaa ccttgcagat
    19141 tgagccgatt attgtgctca acaagataga cctgctggac gacgaaggca tggcgttcgt
    19201 caacgagcag atggatatct accgcaatat cggttatcgc gtattgatgg tttccagcca
    19261 tactcaggat gggctaaaac cgctggaaga ggcgttgacc gggcgcatca gcatttttgc
    19321 cgggcagtct ggcgtcggca aatccagcct gctgaacgcg ttgctggggc tgcaaaaaga
    19381 gatcctgacc aacgatgtct ctgacaactc ggggcttggt cagcacacca cgaccgccgc
    19441 tcggctgtat cacttcccgc acggcggtga tgtgattgat tcccctggtg tgcgtgagtt
    19501 cggcctctgg cacctggagc cggaacaaat cactcagggc tttgtcgaat tccatgacta
    19561 tttaggtctg tgtaaatatc gcgattgcaa acacgatacc gatccgggct gcgctatccg
    19621 ggaagcggtt gaggaaggga aaatcgcgga aacccgtttc gaaaactatc accgtattct
    19681 ggaaagcatg gcgcaggtaa aaacgcgtaa aaacttttct gatacggatg actgacaatt
    19741 aagctaaccg tcgttagaat cgtccccttt ttttcaggat ccggcatgta ggccgaatca
    19801 ggaacgacaa aacaatggcc tggaggctac cttgttaaat tcatttaaac tttcgctaca
    19861 gtacattctg ccgaaactat ggcttactcg cctggcgggt tggggcgcaa gcaagcgggc
    19921 aggatggttg acaaaactgg ttatcgatct gttcgttaaa tactacaagg tcgacatgaa
    19981 agaggcgcaa aagccggaca ccgccagcta ccgcaccttt aacgaattct ttgtccgtcc
    20041 gctgcgtgac gaagtacgcc caatcgatac cgatccgaat gtactggtca tgcctgccga
    20101 tggcgttatc agccagttgg gtaaaatcga agaagataaa atcctgcaag ccaaaggcca
    20161 caactacagc ctcgaagccc tgctggcagg caactatctg atggcggacc tgttccgcaa
    20221 cggtacgttt gtgaccactt acctctcccc gcgtgactac caccgcgtac acatgccgtg
    20281 caacggtatt ctgcgtgaga tgatctacgt gccgggcgat ctcttctccg ttaaccatct
    20341 cacggctcag aacgtgccga atctgtttgc ccgtaacgaa cgcgtgattt gccttttcga
    20401 taccgaattt ggcccaatgg cgcagattct ggtcggagcg acgattgttg gcagcattga
    20461 gacggtctgg gcgggcacca ttacgccgcc gcgcgaaggt atcatcaagc gctggacctg
    20521 gcctgccggg gaaaacgacg gttctgtggc actgctgaaa ggccaggaaa tgggtcgctt
    20581 taaactcggt tccaccgtta tcaacctgtt tgcaccgggt aaagtgaatc tggttgagca
    20641 actggaaagc ctgtctgtca cgaaaatttg ccagccgctg gcagtatcta ccgaaacctt
    20701 tgttacgcca gacgctgaac ctgccccgct tcctgctgaa gagatcgaag cagaacacga
    20761 cgccagccca ttggttgacg acaaaaaaga ccaggtctaa tccatcaaag gaaacgctga
    20821 cgtgcgcctg attatcactt ttctgatggc ctggtgcctc agttgggggg cgtacgccgc
    20881 gacggccccc gatagcaaac aaatcactca ggaactggag caggcaaaag cggcgaaacc
    20941 cgcacagccg gaagtcgtag aggcgcttca gtctgcctta aatgcgcttg aggaacgaaa
    21001 aggttccctt gagcgcatca aacaatatca gcaagttatc gataattatc cgaaactctc
    21061 cgctactctg cgcgcacaat taaacaacat gcgtgacaag ccgcgcagcg tgtcgccggg
    21121 aatgtctacc gacgcgctga atcaggaaat tctccaggtc agcagccagt tgctggataa
    21181 aagccgtcag gcccagcaag agcaggagcg cgcccgcgag attgccgatt cgctgaatca
    21241 actgccgcaa cagcaaaccg acgcccgccg tcagttaaat gagatcgagc gccgcctggg
    21301 aacgcttacc ggcaatactc cgctcaatca ggcacaaaat ttcgcgttgc agtctgactc
    21361 tgcacgtctt aaggcgctcg ttgatgaact ggagctggcg cagctgtctg ccaataaccg
    21421 ccaagaatta gcgcgcttac gctcagagct tgcggaaaaa gagagccagc aactggatgc
    21481 gtatttgcag gccttacgta atcagttgaa tagccaacgt cagcttgagg cggagcgggc
    21541 gctggaaagt accgaattac tggcagaaaa cagtgccgat ttgccgaaag atatcgtcgc
    21601 gcaattcaaa attaaccgcg aactatcggc agcgctgaat caacaggcgc agcggatgga
    21661 tctcgttgcc tcgcaacagc gtcaggctgc cagccagacg ttacaggtcc ggcaggcgtt
    21721 gaatacgctg cgtgaacagt cgcaatggct gggatcgtcc aatctgctcg gtgaagcgtt
    21781 gcgagcgcag gtggcacggc taccagaaat gccaaaacca caacagcttg ataccgaaat
    21841 ggcgcagttg cgtgtgcaac ggttacgtta tgaggatctg cttaataaac agccgctgct
    21901 acggcaaatc catcaggccg acggtcagcc gctgaccgcc gagcaaaacc gtattctgga
    21961 agcacaactg cgcactcagc gtgagttgct gaactcattg ttgcagggtg gcgacacgct
    22021 actattggaa ctgaccaagc tgaaagtctc caacgggcaa ctggaggatg cgctgaaaga
    22081 ggtgaacgaa gcaacgcacc gctatctgtt ctggacctct gacgtgcgcc cgatgaccat
    22141 cgcctggccg ctggaaatcg cccaggatct gcgtcgtctc atttcgctgg acaccttcag
    22201 tcagttgggc aaagccagtg tgatgatgct gaccagcaaa gagacaattt tgccgctgtt
    22261 tggcgcgttg attctggtcg gttgcagtat ttactcgcgc cgctatttca cccgttttct
    22321 tgaacgttcg gcggcgaaag ttggcaaagt gactcaggat cacttctggc tgacgttgcg
    22381 cactcttttc tggtcgattc tcgtcgcgtc accgttaccg gtgctgtgga tgacgctggg
    22441 ttacggcttg cgcgaggcgt ggccttatcc gctggcggtc gcgattggcg atggtgtaac
    22501 ggccaccgtg ccgctgctgt gggtagtgat gatttgcgcc acctttgccc gcccgaacgg
    22561 cttgtttatc gctcattttg gctggccgcg cgaacgcgta tctcgtggga tgcgctacta
    22621 cctgatgagc atcgggctta ttgtgccgct gattatggcg ctgatgatgt tcgataacct
    22681 cgacgaccgt gaattctccg gttcgctggg aaggctttgc tttatcctca tttgcggtgc
    22741 gctggcggtg gtcaccctca gcctgaaaaa ggccgggatt ccgctgtatc tcaacaaaga
    22801 aggcagcggc gacaacatta ccaaccatat gctgtggaac atgatgattg gcgcgccgtt
    22861 ggttgccatt ctggcgtcgg ctgtgggtta tctggcaacg gcacaggcgc tgttagcgag
    22921 gcttgaaacc tcggttgcca tctggttcct gctactggtg gtttatcacg ttatccgccg
    22981 ctggatgctg atccagcgcc gcaggctggc gtttgaccgg gcgaagcatc gccgggcaga
    23041 gatgttagcg caacgtgcgc gtggcgaaga ggaagcgcat catcacagta gcccggaagg
    23101 agcaattgaa gtcgatgaaa gcgaagtcga tctcgatgcc atcagtgcgc aatccttgcg
    23161 gctggtgcgc tcaattttga tgttgatcgc cctgctttct gtcattgtgc tgtggtcaga
    23221 aatccattcc gctttcggct tcctcgaaaa tatttcgctg tgggatgtca cctccacggt
    23281 acagggcgta gaaagtctgg agccaattac cctcggtgcg gtgctgattg ccattctggt
    23341 gtttatcatc accacgcagc tggtgcgcaa cctgcccgcg ctgctggaac tggcgatttt
    23401 gcagcacctg gatttaacgc cgggtacggg ctacgccatc accaccatca ccaaatatct
    23461 gctgatgctg attggcgggc tggtcggctt ctcgatgatt ggtattgagt ggtcgaaatt
    23521 gcagtggctg gtcgccgcgc tcggtgttgg tctcggtttt ggtttgcagg aaattttcgc
    23581 caactttatc tctggtctga ttatcctgtt cgaaaaaccg attcgcattg gcgatacggt
    23641 gacaattcgc gatctcaccg gtagcgtgac gaaaattaac acccgcgcca ccaccatcag
    23701 cgactgggac cgtaaagaga taatcgtgcc gaacaaggcg tttattaccg agcagtttat
    23761 caactggtcg ctctctgact cggtcacgcg cgtagtgttg acgattccgg cccctgctga
    23821 tgccaacagt gaagaagtga cggaaatcct gctcaccgca gcgcgtcgct gctcgctggt
    23881 gatcgacaac ccggcaccgg aagtcttcct ggtggatctg caacaaggga ttcagatttt
    23941 cgagctgcgt atttatgccg ctgagatggg tcaccgtatg ccgctacgcc atgagatcca
    24001 ccagctgatt ctggctggct tccatgccca cggtatcgat atgccattcc cgcccttcca
    24061 gatgcgtctg gaaagcctca acggtaaaca aacggggaga acgctgacgt ctgcgggcaa
    24121 aggtcgtcag gcgggaagtt tgtaatgcat gaagctgcca aatgatgtca acgtggctca
    24181 tttagcagct taacatcatc ttcagttatc accggccctg gagagtatca tccatacgca
    24241 ttacttcctg acaactcatc gccggagaga cgatgagttg caggatttca tatgatcagt
    24301 cgagagctaa ctcaggttta ttcgccatgc gactgcaata gttctggtaa atcgccatcg
    24361 ctaacagcga gaagaagaca gggccgccaa tcatccacaa tgtgctgtcc cagtctccgg
    24421 cttcaaccac aggctgaata atggtgaaga cgttcgcaaa tgtcaccacc agtacgacaa
    24481 ccaccatcgc aatcattgcc gacatacgcg ttttgaaaat cacaaatggt ctgtcgagat
    24541 cctgacgtgc tttaaagaat gggaaagcca gcgcgaggaa caggtaagga agcgtcatag
    24601 acacgttcgc catcagcgtc agcttgttaa agaacgcgga tgcggtaccg ccaccaaacg
    24661 aaaccagcag gatgaagaca gtaaccaacc cgcactgcat ccacatggcg atagacggca
    24721 tccccatcgc attcaggcgc gtcatcggtt ccggccacaa cgctttcggc gtcccctgga
    24781 tgatggcttt cagcggtgaa tagcaaagcg taaagaacgc accggtataa gcgaggaaca
    24841 tcgaaagtcc ggtaatacgc gcaaaccata cgcccagcga caatgacgct tcaggtgaca
    24901 aatgcagtgc gttacccagc gtcaccccga ggctcttcat cagcacatag gtaatattgc
    24961 caaggttaac ggaaccatta cttaatacct gctgccagtt tgtgctgacg ccccataaaa
    25021 atattgccag cgaataaccg attgaaataa caatagcggc aaaaacaata cctttggcaa
    25081 agttcttttc tggattttcc gttttatcga ccagaccacc aaccgcttca attccgccat
    25141 aggcaaaaat agcaaatacc acaaacgata gcattgccag accggactga taacccgggt
    25201 tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc accatttaat aacaaaatag
    25261 taatgcttac taacagcaat actaaattca gacacattac tgcaataccg cccaccgcag
    25321 taatgcgggc aattttatta atcccctttg aagcaacgac ggtgaccaga atcatccatg
    25381 ctactgccag cagaccaacc acctgcgtag gctccagtcc ggcaatacgc cagtgctggg
    25441 tcatgtcgct accatagagg aatgttgaga acggtaccca aactttcgcg gaggtactca
    25501 ccatccagat gatataagag gaaaaccaca taaacgtacc aatgaaggca aaacgtggtc
    25561 cgacactatt attcatccag gaatagatac caccttcttc tttgcgataa gcagctccca
    25621 tttcagccat cattaa
//
LOCUS       DAQSWQ010000053        25449 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.053, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000053 DAQSWQ010000000
VERSION     DAQSWQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.053"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="R8G18_002317"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="R8G18_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HEE9795996.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="R8G18_002318"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="R8G18_002318"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HEE9795997.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="R8G18_002319"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="R8G18_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="R8G18_002320"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="R8G18_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HEE9795998.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="R8G18_002321"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="R8G18_002321"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEE9795999.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="R8G18_002322"
     CDS             complement(6490..6846)
                     /locus_tag="R8G18_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEE9796000.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="R8G18_002323"
     CDS             complement(6850..7266)
                     /locus_tag="R8G18_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEE9796001.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="R8G18_002324"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="R8G18_002324"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEE9796002.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8732
                     /locus_tag="R8G18_002325"
                     /pseudo
     CDS             8492..8732
                     /locus_tag="R8G18_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9236..10429)
                     /gene="tyrB"
                     /locus_tag="R8G18_002326"
     CDS             complement(9236..10429)
                     /gene="tyrB"
                     /locus_tag="R8G18_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HEE9796003.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10682..11761)
                     /gene="alr"
                     /locus_tag="R8G18_002327"
     CDS             complement(10682..11761)
                     /gene="alr"
                     /locus_tag="R8G18_002327"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEE9796004.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11814..13229)
                     /gene="dnaB"
                     /locus_tag="R8G18_002328"
     CDS             complement(11814..13229)
                     /gene="dnaB"
                     /locus_tag="R8G18_002328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEE9796005.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13312..14295
                     /locus_tag="R8G18_002329"
     CDS             13312..14295
                     /locus_tag="R8G18_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEE9796006.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14461..14703)
                     /gene="pspG"
                     /locus_tag="R8G18_002330"
     CDS             complement(14461..14703)
                     /gene="pspG"
                     /locus_tag="R8G18_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HEE9796007.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14837..15874)
                     /gene="dusA"
                     /locus_tag="R8G18_002331"
     CDS             complement(14837..15874)
                     /gene="dusA"
                     /locus_tag="R8G18_002331"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEE9796008.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16237..17222)
                     /locus_tag="R8G18_002332"
                     /pseudo
     CDS             complement(16237..17222)
                     /locus_tag="R8G18_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17540..18055
                     /gene="zur"
                     /locus_tag="R8G18_002333"
     CDS             17540..18055
                     /gene="zur"
                     /locus_tag="R8G18_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HEE9796009.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18097..18306)
                     /locus_tag="R8G18_002334"
     CDS             complement(18097..18306)
                     /locus_tag="R8G18_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEE9796010.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18422..19747)
                     /gene="dinF"
                     /locus_tag="R8G18_002335"
     CDS             complement(18422..19747)
                     /gene="dinF"
                     /locus_tag="R8G18_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HEE9796011.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19820..20428)
                     /gene="lexA"
                     /locus_tag="R8G18_002336"
     CDS             complement(19820..20428)
                     /gene="lexA"
                     /locus_tag="R8G18_002336"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HEE9796012.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20538..20906)
                     /gene="dgkA"
                     /locus_tag="R8G18_002337"
     CDS             complement(20538..20906)
                     /gene="dgkA"
                     /locus_tag="R8G18_002337"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEE9796013.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21077..23500
                     /gene="plsB"
                     /locus_tag="R8G18_002338"
     CDS             21077..23500
                     /gene="plsB"
                     /locus_tag="R8G18_002338"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HEE9796014.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23655..24527)
                     /gene="ubiA"
                     /locus_tag="R8G18_002339"
     CDS             complement(23655..24527)
                     /gene="ubiA"
                     /locus_tag="R8G18_002339"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEE9796015.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24540..25037)
                     /gene="ubiC"
                     /locus_tag="R8G18_002340"
     CDS             complement(24540..25037)
                     /gene="ubiC"
                     /locus_tag="R8G18_002340"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEE9796016.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25260..>25449)
                     /locus_tag="R8G18_002341"
     CDS             complement(25260..>25449)
                     /locus_tag="R8G18_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796017.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
BASE COUNT         6587 a         6545 c         6090 g         6227 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgccaaag atttttctta tgctaaagat ttctttttat acatagaaac
     8641 acagttaaaa attgcaaaag atttttgtag acctggagaa gaagtatcaa gttctattgc
     8701 aagtaaagtt tttcacgcat ttatcgattt agttaacaaa atcaggggta agaaagactt
     8761 tatgtatatt tgcacgcttt gctgttttgc cgaggaggtt aaaggtgatt attctcatta
     8821 caggaccttt ttatttgata ttggtaatca atataaggtt aaacttacac aaagcggaaa
     8881 aaaagagttc tctttaacat tagaatttaa cgatactata attgaatctc agaaagtcac
     8941 aggcaataag gcaaagtata ttcttgagga tatagaaata ttctatcgta acaaacccga
     9001 tacttattat taaatttaat aatggttaga tgtgcagaaa ttattactcc acacgaaaaa
     9061 atagggaaag gaattccaga taattgggat aaacttaaca ataaaagaac tgacaacgtt
     9121 ttattatctc tcacgtagaa caacggcatc aaaaaaatca tctacaaaga aatagtgaaa
     9181 gcgacgacag tttaatttca ctgcaggctg ggtaactcca gcctgctttc ctgcattaca
     9241 tcaccgcagc aaacgccttt gccacacgtt gtacatttgc cgtatttaac ccggcgacac
     9301 acatgcgacc gctggcgatg agatagacac caaattcttc acgtagtcgg tcaacctgag
     9361 cggcacttaa accggtataa ctgaacatgc cgcgctgatt aagcagataa tcgaaattgc
     9421 gttctggcat ctctgtgctt aataccttca ccaattcctg acgcattgcc agaatgcgag
     9481 tacgcatctc ttctacttcc gccagccagc tggctttcaa tgcctcgtca ttcagcactg
     9541 cagccaccac ctgcgcacca aaattcggcg ggctggagta gttgcggcga actgttgctt
     9601 tcaattgccc cagtacgcgg ccagcggctt cggcatcttc acacagaaca gaaagtccgc
     9661 cgacgcgctc gccgtaaagg gagaaaattt tcgagaacga attgctcacc agagcgggta
     9721 atccagcgct ggcaatggcg cgaatagcgt aggcatcctc ttccataccg gcactaaatc
     9781 cttgataggc aatatcgagg aatgggataa gctcgcgggc tttgagaatt tcaatcaccg
     9841 catcccactg atcattagtg agatcggcac ccgttgggtt gtggcaacat ggatgcagca
     9901 acacaatact gcgggcaggt aatgttttca gcgtcgccaa caggtcatta aagcgcacgc
     9961 tgttagtcgc ttcgtcatac caggggtaag tactcacttc gaatccagcc ccggcgaata
    10021 ttgctacgtg gttttcccag gtaggatcgc tgacccagac gcctgattcc gggaagtagc
    10081 gtttcaggaa atccgcgccc actttcaatg cccctgagcc gccaagggtt tgaatggttg
    10141 ctacgcgctg ttgttgcagt accggatggt ccgcaccaaa cagcagcggc gcaatggcat
    10201 ggcgatagct gttaagccct tccatcggta aataaagcga agcgccatgg ggctgcgcat
    10261 tcaggcgcgc ttccgcctcc gccacggctt tcagttgtgg aataattccg tcttcgttgt
    10321 agtacagacc gatacttaaa ttcactttgt cgctgcgagg gtcttcttta aaacgctcca
    10381 taagcgtaag aatcgggtcg ccagcgtagg cgtcaacttt ttgaaacacg cgatggttct
    10441 ccaggtttac gggcaggtgg ttaaaacaca ataaaccgga agaaggcgaa gatcgagtgg
    10501 atgttcagga gcgaacggca attagcaaca gagtgagact catgacaaac gaacatccgc
    10561 cagaggcacg acctttataa gagcatgaaa gaacatcaac ttattgaatt tttaggattt
    10621 tattggccgg ataaggcatt cacgccgcat ccggcacaga caatcaaata ttacagaacg
    10681 attaatccac gtatttcatc gcgacccttg aagtcaggcg cgtaataagt tcgtaagcgc
    10741 tcacttttgt catttcagcg atacgttcta cgggcaaacc ttcgccccat aaaatgaccg
    10801 gatccccggc tttgtcctgc gcctgtggac ctaagtctac gcagatcata tccatcgcca
    10861 cgcgtccgac aatcggtact tcgcgaccgt tcaccagcac tggcgtaccg gatggagcgg
    10921 cgcgcggata accatcgcca tagcccatcg cgactacgcc aagacgggta tcacgttcgc
    10981 ttacccaggt tccaccataa ccaacaggct ctccggcttt atgctcacgc acggcaatca
    11041 ggctggaggt tagtgacatc actggctgac agccaaaatc ggcaccgatg gagcgatctt
    11101 ccagcggcga gacgccataa agaatgatgc ccgggcgcac ccagtcaaaa tgcgattgtg
    11161 gccacagcag aatgccaccc gacgcggcaa tggaacgttg accaggtttg ccttcgcaaa
    11221 aggtattaaa gatagcgagt tgtttctcgg ttgcgccaca ttttggttca tccgcgcgcg
    11281 caaaatggct gacgatattc accggctgac gaacgttttt gcactgtgtc aggcgatgat
    11341 aaaacgcctc agcctgttcc ggccttacgc ccagacggtg cataccggta tcgagtttca
    11401 tccagacggt aaccggctcg tccaggctag cctcttccag cgcagccagc tgttcttcgt
    11461 tatgcacggc ggtatgaaaa tgttgcgcag aaatcgtcgg cagatctctg gcatcaaaaa
    11521 agccttcgag taacagtaca ggtttggtga ttccccccgc acgcagtcgc agagcttctt
    11581 cgagacgggc tacgccaaag gcgtcagcat cggggagcgt tcgcgcggtc tcaagaagac
    11641 cgtgaccata agcgttcgct ttcaccaccg caaccatttt actggcaggg gccagttcac
    11701 gaagacgttg caggttgtgt cgcagagcgc ggcggttaat cacaacagtt gccgcttgca
    11761 tttgtgttcc ttgataagtg tttgctttaa ttacctaatt cataaaataa ttattattcg
    11821 tcgtcgtact gcggccccgc atagttgtcg aagcgcgacc attgaccgtt aaaggtcagg
    11881 cgtaccgtcc cgattgggcc gttacgttgt ttaccgataa taatttccgc gatgcctttt
    11941 aaatcactgt tttcgtgata cacctcatca cgatagataa acataatcaa gtccgcatcc
    12001 tgctcgatag agccagattc acgcaggtcg gagttgaccg ggcgtttgtc ggcacgttgt
    12061 tccagagaac ggttcaactg ggacagcgcc accaccggca cgttcagttc tttcgccagt
    12121 gctttcagcg agcgagagat ttctgcaatt tccagcgtac ggttatcgga aagcgccggt
    12181 acgcgcatca gttgcaggta gtcgatcatg ataagcccga tgccgccgtg ttcacgggca
    12241 atacggcgtg cgcgggaacg cacttccgtt ggcgtcaggc cggaggagtc atcgatatag
    12301 atattgcgtt tttcgagcaa aatacccatg gtgccggaaa tgcgcgccca gtcttcatca
    12361 tcgagctgcc cggtacggat tttagtctgg tcaacgcgcg acagcgacgc cagagaacgc
    12421 atcataatct gttctgaagg catctccagc gagaagataa gtactggttt atcctgcaac
    12481 atcgccgcgt tttcgacgag gttcatcgca aatgttgttt tacccatcga cggacgcgcg
    12541 gcgacgatga tcaaatccga cggctgcaag ccagcggttt ttttgttgag atcgtcataa
    12601 ccggtgttta ctccggtaac gccatcgtgt ggctgctgaa acaactgctc aatacgcgcc
    12661 acggttgcgt cgagcacatc ggcgatgttc ttcggccctt cgtctttgtt tgcacgactt
    12721 tcggcaattt taaagacgcg ggattcagca aggtccagca gatcttcgct ggtacgcccc
    12781 tgcggatcaa aaccggcttc agcaatctca ttcgcaaccg agatcatctc acggacaacg
    12841 gcacgttcac gcacgatgtc agcataagca ctgatgttcg ccgcacttgg cgtatttttt
    12901 gacagctctg ccagataagc aaaaccaccg acgctatcga gttgcccctg gcgttccagc
    12961 gattccgcaa gggtaatcag atcgatagga ctaccgcttt cctgcaaacg cgccatttca
    13021 gtaaagatat ggcggtgtgg gcgggtgtaa aaatcgtctg ccaccacacg ctcggctaca
    13081 tcgtcccagc gttcgttatc cagcattaaa ccgcccaaca ccgactgctc cgcttcgatc
    13141 gagtgcggag gcactttcag cccggcaact tgtggatcgc gttcgcgggg ttcagcctgc
    13201 tgtttgttga agggtttatt tcctgccata gtgaatggag ttaccgagat aaagaatggg
    13261 tcgaaacttt accatatgaa gcagaccctg acgatacgtt ctggaggaca catggcaaca
    13321 cgaattgaat ttcacaagca cggtggcccg gaagtacttc aagccgtaga gttcactcct
    13381 gccgatccgg cagagaatga aatccaggtc gaaaataaag ccatcggcat caattttatc
    13441 gacacgtata tccgcagcgg cctttacccg ccgccatcac tacccagcgg attaggtacc
    13501 gaagcggcag gcatcgtgag taaagtcggc agtggtgtaa agcatattaa ggcaggcgat
    13561 cgtgtagtct atgcgcagtc ggcgttaggc gcttacagct ctgtgcataa cattaatgcg
    13621 gataaagcgg cgattctgcc tgcggcaatt tcttttgagc aagctgcggc atccttcctg
    13681 aaaggcttaa cggtttatta tctgctgcgc aaaacctatg aaattaaacc cgatgaacag
    13741 ttcctgttcc acgcagcggc tggcggcgtt ggcttaattg cctgccagtg ggcaaaagcc
    13801 ctgggcgcga aacttatcgg caccgtagga accgcgcaaa aagcgcagag cgcgctaaaa
    13861 gcgggcgcgt ggcaggttat taactatcgt gaagaggatc tggtcgagcg gttaaaagag
    13921 atcaccggcg gtaagaaagt gcgcgtggtg tacgattccg tgggcagaga cacctgggaa
    13981 cggtcgctgg attgcctgca acgccgcggc ttaatggtca gttttggcaa ctcatcaggt
    14041 gcggttaccg gtgtgaactt aggcattctc aatcaaaaag gctcgttgta tgtgacacgc
    14101 ccttccctgc aaggctatat caccacgcgg gaggaattaa ccgaggccag taatgaactg
    14161 ttctctttga ttgccagcgg tgtgattaag gtcgatgtcg ccgagcagca gaaatatccg
    14221 ctgaaggatg cgcagcgtgc gcatgagatt ctggaaagcc gggcgacgca aggttccagc
    14281 ctgttgattc cataaaagaa atagggcttc cacctgggaa gccctttctt tttatagttc
    14341 ggctgtatgt agggtacagc acgatgaatc tgttagaggc gcaatagtga cagatttgat
    14401 tatcaattcc tattttgttc taaggataaa accttaggtt gtgatcatcc gcacaatccc
    14461 ttagtaacgc cagcggtcat aacgctgata tttcggcact tttggtgctt taatcgcctt
    14521 aataacccac accaccgcaa tcgccagtag taaccacggc agcaacttaa tcatcaatgc
    14581 cagcataccg ccgaggaaca taatggccgt cgccacaacc agcgcggcga taatgcccag
    14641 caacgaaacg ccggtgacca tcagcatgac aaaaaagcca atcacaaaaa gtagttccag
    14701 catgatgctc tcccaaatat gaaatctctt gctggcatta caagaatcat gccaaaaata
    14761 atctattgat ttaacagcaa aacgccccgc gacggtgcgc agggcgtggt gaatttgact
    14821 actttttggt gaaaagttaa cgcttatccg ccaccagttt gagcgcgtgt tccagcacat
    14881 taatgtctgc acccgcttta tgggcatttt cacttaaata acgccgccac tgccgcgcgc
    14941 caggaatacc ctggaacaag cccaacatat gccgggtaat atggccgaga tacgtcccct
    15001 ggctgagttc acgctcaatg tacggataca tggcgcgcac taccgccacc ggatcggcat
    15061 cggtatccga ggaaccaaag atctcccggt ctaccgccgc cagaataccc ggattctgat
    15121 acgcctcgcg cccgaccatc acgccatcca tatgttgcag gtgtgctttg gcctcttcca
    15181 gcgacttgat accaccgtta atcgacattg tcagatgcgg aaagtcacgc ttcagttgat
    15241 acacacgcgg ataatcgagc ggcgggattt cacggttttc tttcgggctt aaccccgaaa
    15301 gccaggcttt acgtgcgtgg atgataaaca tctcacactc gcctttgccg gaaacggtgt
    15361 tgatgaaatc gcagagaaat tcatagctgt cctggtcatc gatgccaata cgcgttttca
    15421 ccgtcactgg aatcgacacc acatcgcgca tcgctttcac gcagtcggca accagctgcg
    15481 cattacccat cagacacgca ccaaacatgc cgttctgcac ccggtcagac gggcagccga
    15541 cattcaggtt gatctcatca tatccacgcg cttctgccag cttcgcacac tgtgccagcg
    15601 ccgccggatc gctaccgccg agttgcaacg ctaccggatg ttcttcttca ctgtacgcca
    15661 ggtaatcacc tttaccgtga ataatcgccc ctgtggtcac catttcggta tacagcaacg
    15721 tattgcggga aagcagacgc aagaaatagc ggcaatgtct gtccgtccag tcgagcattg
    15781 gtgcaacgct aaaccgacca ctccagtgaa cgtcagtttt ttcaggcatt gcgctggttt
    15841 ggttgatttt ttgcatttca gaattaccgt gcattttcaa atgtagagat tattttatcg
    15901 atatatcatg gggttatgtt attcagcatc actattcagg aggctcaata gcggggtact
    15961 ataccataac aacaggaagc gcctgtctca ttgcaaaaga aaattgagat cctctcaagg
    16021 catgaagctc tcacgaagtg atggaaataa tcttattagc cgttaacttt gttaaggcca
    16081 atgataaaca atccaggttc gacgataaat aaaaaatcac acattaaact ctggtgatat
    16141 atctccctgc taatagcatt gatagagaaa aaagaaccca ataaatattg ggtcctttta
    16201 tataatgcct tccatactat cgaagaactt cacatattat ttctccgatt taaccccgaa
    16261 caaatcataa attaatttag aagtgtctgt aagtatttta atctcttcct ttgaggttgg
    16321 gtcaaacgac ttcgcgaagc taattaattg tggtgcagca tctcgcattt tgcttaaaat
    16381 atcaggatcg agcgttcctt cattcaccag atgtgacatc ttatccagat agccatcaaa
    16441 attcatttct cccccatcag ccagacgctt catctctccc aggtacttct tcatatcacg
    16501 ttgagataat ttttcaaact gagctttcag gtagctctca ttattttcat taatatacac
    16561 tgtttctgat aaatctgcgt aggcactaga atatgaaagt gaaattaaca gcgaagcaaa
    16621 taattttaag ctgttgtcat catgttcctg gcaggcatta acaaacgtta aaaaaccaga
    16681 acctattgct tgaaaatgta tattcgctaa aggttcatta tccttagatt cctcataaaa
    16741 tagctgaacc gtggagggaa ggccattctt agtttctaca tcaaaagtac attgttcaat
    16801 gggagtcgca tctttattat ccccaggatt acatatacta ttaatcgcga aatgaaaaat
    16861 agcccggtca acaactgatg caataaagtc acgatcgttt aaatcctcat cagtgcaatc
    16921 gtcaatatac ttattgaccc acattttgag atcatttatt tgctcaatta aaaacacatt
    16981 tttttacgtg catgccataa attatgcgtg attcgttgaa taaacaaatc tatttttgca
    17041 ataattatat cccgatgacg cagcgctgca taatcaatag atttaccata tatattattt
    17101 aatttacctt tgattgaacc tctattatct aatttatctt tgattgcatc ttcattatcg
    17161 aattgatttg aattgagtgt tttattaaag tatccagttg caccgggtat tatcttcaac
    17221 atgattcatc tacctttttt gtcgtaccgt gtcctcctta taattctttt gccatctggt
    17281 cactatcaaa aatcgctcat aatttctgaa ctacaggtat gcgtttaaac ccagttaaat
    17341 aaatagcgag ttaacttgaa gcaatttatt taaaaatgaa ttattaatta ttatcgtgtt
    17401 gataatggta cgacaaggca acataacacc ttacctacaa aacagaaaat caacattacg
    17461 gcaacaataa gggttctcgg tgtttttcct ggaacatggt aaagtaagga cattcttaac
    17521 ccccactttg aggtgcccga tggaaaagac cacaacgcag gagttattag cgcaggctga
    17581 aaaaatctgc gcgcagcgta atgtgcgcct gaccccacag cgcctggaag tgttgcgcct
    17641 gatgagtctg caggatggcg ctatcagcgc ttatgatctg cttgatttac tgcgcgaagc
    17701 tgaaccgcaa gccaagccgc caacggttta tcgcgcgctg gattttctgc ttgagcaagg
    17761 ttttgtgcat aaggtggaat ccaccaacag ttatgtgctc tgtcatctgt tcgaccagcc
    17821 cacgcatacg tcagccatgt ttatatgcga tcgctgcggc gcggtgaaag aagagtgtgc
    17881 agaaggcgtg gaagacatta tgcatacgct ggcggcaaaa atggggtttg ccctgcggca
    17941 taatgtgatt gaagcacatg ggctctgtgc ggcatgcgtg gaagtggaag cgtgtcgtca
    18001 tcctgaacag tgccagcatg atcactctgt gcaggtgaaa aagaaaccgc gttaagaggg
    18061 cgtacatcct tgtacacgtc gggcaggagg gattaattac cagcgatatt cattgcgggt
    18121 ttcccaatcc acgacctctt tttctgcctg atctttctga taaccataac gttcctggat
    18181 tttaccgacc agttgatcac gtttaccttc aatgatcgtc atatcatcat cggtcagttt
    18241 gccccattgc tctttcactt tacctttaaa ctgtttccag ttaccgccgg cttcatcttt
    18301 attcataatc aagacctcat cgttaggttg tgaatgagag tacgttcact tttcttctga
    18361 acgtgagatt aagtgtagtc agcaatttgg cttaggataa tttattcaga atttttaacc
    18421 gtcacgttgc ggcaaaccag gtaccgttac gccagtgacg ccgccagata gccgccagag
    18481 aaagcccgcg caacgccaga aagacggtta atgccagcca caaaccatga ttacccagcc
    18541 acggcagcgt aaggagcgtc agcgcaaaac ctgcggcggc caccgccata ctgttacgca
    18601 tttcggcggc acgcgttgcg cctataaaca tgccgtccag cagataacac cagacgccaa
    18661 ccaacggcaa aatcacctgc cagataagat agcggtcagc cagctgctga atctgggtta
    18721 acgacgtcag caacgcaatg atgtgttccc ctgccagcaa ataaaccacc gaaaatagta
    18781 acgctacgat ccccgactgg cggcacgctg cccgccagac atccagcaac tggctaccgt
    18841 cgcgcgcacc gtatgcctga ccggaatgtg cttcaaccgc gtaggcaaaa ccatccagcg
    18901 cataggcggt aaaggtgagt agcgtcatca gaaccgcgtt tacagcgata atgtcactcc
    18961 ccagtcgcgc gccaagtacg gtgatcgcgc cgaaacagag ttgcaacaac agcgaacgca
    19021 gcatgatatc gcggttaagc gccagcaagc gacggaagtt tcctcgccag gcagttttcg
    19081 acatttcgcc ggaaattccg cgtagtttga ggattttacg caccattagc agaccaatca
    19141 gcaatgttgc atattccgca ataaccgtcg ccagagccgc gccctgcacg ttcatatgca
    19201 gccccatcac tagccagaca tccagcacaa tgttgaggat attgccgacc actaacaaaa
    19261 ttactggcgc acgggcatat tgcacgccga gtaaccaacc gagtaatacc agattcgcca
    19321 gcgacgccgg tgcgcttaac cagcggattt caagaaagcg ccgcgcctgt tccaggactg
    19381 cttcactacc gccaacaata tgcagagcca gatcgataat cggtgtacgc agcagcgcaa
    19441 ttaacgcccc agcccccaac gccaacagca acggttgcac cagcgcacgg gctaatgcct
    19501 gaggattttt ggcaccataa gcctgcgcag tcagcccggt ggtgctcatg cgtaaaaaca
    19561 gcaacagcat aaagagaaag ctggtcgccg ttgcaccaac cgccacgccg cccaaataaa
    19621 ccgggctatc aagatgacca attaccgccg tatcgaccag tcccagcaac ggaacggtga
    19681 tattggagaa aatcatgggt aaggcgagat gccagagtgc tttatcagat gaagtgagga
    19741 atgccatgca gacaagcctg atgaagagag atgaaaaaca aaccgcgacg cctggcggca
    19801 tcgcggtctc agagatatgt tacagccagt cgccgttgcg aataacccca accgccagcc
    19861 cttcaatggt gaagctctgc tgacgaaggt caacgacaat tggtttaaac tcgctatttt
    19921 ctggcaacag ttcgacttta ttaccctgtt ttttcaggcg cttaacggta acttcgtcat
    19981 caatacgtgc gacaacgacc tgaccgttac gtacatcctg agttttatgc actgccagca
    20041 agtcaccatc cataatgccg atatctttca tcgacatccc gctgacgcgc agcaggaaat
    20101 cagcattcgg cttgaacaag gaaggatcga cctgataatg accttcaata tgctgctgcg
    20161 ccagaagcgg ttcaccggca gccacacgac ctaccagcgg caacccttct tcctcttcct
    20221 gcaacagacg aatcccgcgt gatgcgccgg aaacaatttc aataacgcct ttgcgtgcca
    20281 gcgccttcag atgttcttca gccgcgtttg gggaacggaa ccccaaacgc tgcgcgattt
    20341 ccgcacgcgt cggcggcata cctgtctggc tgatgtgatc acggatgaga tcaaacacct
    20401 cttgttgcct ggccgttaac gctttcattc cgccccctgg gtgtatatac agttatgctg
    20461 tgagtatata cagcaaaagg cgattttgga accataaact gcacaataaa ccagagattt
    20521 atcgaaatct ggaagggtta tccaaaatgc gaccataaca ggatgcacca ggttatcacg
    20581 gcgacgataa tcgcaatcag caccgcggcg gatcccatat ctttagcgcg tccggaaagc
    20641 tcatggtatt cagagccaat tcggtcaacc actgcttcga tggcgctatt gagaatttcc
    20701 acaatcatca ccagcatcac ggagctgata agcagcacgc gggtaattgc gtccacatcc
    20761 agccagcagg cgatgaccac cgccaacaat accgctacgc cttcctgacg gaatgccgct
    20821 tcgttgatcc atgcagcgcg taaacctttc caggaatagc cagcagcttt gataattcgg
    20881 gtgaatccag tggtattatt ggccattgaa agaacctttt tacattatga gcgtcaatat
    20941 cagtgtaccg ttaaatccga tgattcgggt ttgcgtaacg ccgcgcgaaa catgagcgga
    21001 taccacagaa tttcccatga ctttctgcta tccttgccgc gcatttgcat tattaaccag
    21061 aggctttaca tcgtttatgt ccggctggcc acgaatttac tacaaattac tgaatttacc
    21121 attaagcatc ctggtaaaaa gcaagtctat tccggcagat cctgccccgg aactggggct
    21181 ggatacctct cgtccaatta tgtacgtttt accgtacaac tcgaaagcag atttgctgac
    21241 gttacgcgcc cagtgtctgg ctcatgactt gcctgacccg ttagagccgc tagaaatcga
    21301 cggcacgcta ctgccgcgct atgtgttcat tcacggcggg ccgcgtgtat tcacctatta
    21361 cacgccgaaa gaagagtcta ttaagctgtt ccacgactat ctcgatttgc accgtagcaa
    21421 cccaaatctg gatgtgcaga tggtgccagt atcggtgatg tttggtcgcg cgccggggcg
    21481 tgaaaaaggc gaagtaaacc cgccgctgcg tatgcttaac ggcgtacaga aatttttcgc
    21541 cgtactgtgg ctcggtcgcg acagttttgt gcgtttctcg ccgtcagttt cgctgcgccg
    21601 tatggcggat gaacacggca cggataaaac tatcgcacag aaactggcgc gcgtggcgcg
    21661 tatgcacttt gcccgtcaac gtctggctgc cgtaggccca cgtctgcctg ctcgtcagga
    21721 tctgtttaat aagctgctcg cctcccgcgc cattgccaaa gcggtagaag atgaagcgcg
    21781 cagcaaaaaa atctcccatg aaaaagcgca gcagaacgcg attgcgctga tggaagagat
    21841 tgcggcgaat ttctcttacg agatgatccg cctgactgac cgcattctgg gcttcacctg
    21901 gaaccgactt tatcagggca tcaacgtcca taacgccgag cgcgttcgcc agctggccca
    21961 cgacggtcat gagctggtgt atgtgccttg ccaccgcagt cacatggact acctgctact
    22021 ttcttacgtg ctgtaccacc aggggctggt gccgccgcat atcgccgccg ggatcaacct
    22081 gaacttctgg ccggccgggc cgattttccg ccgtctgggg gcgttcttta ttcgccgtac
    22141 ttttaaaggc aataaacttt attccaccgt tttccgtgag tatctcggcg aactgttcag
    22201 ccgtggttat tccgtcgagt acttcgtgga aggcggtcgt tcccgcacgg ggcgtttgct
    22261 ggatccgaaa accggtacgc tgtcgatgac cattcaggcg atgctgcgtg gcggcacgcg
    22321 tccgattacg ctgattccga tctatatcgg ttatgagcac gtcatggaag tgggtactta
    22381 cgccaaagaa ctgcgcggcg cgacgaaaga gaaagagagc ctgccgcaga tgctgcgcgg
    22441 tttaagcaag ctgcgtaatc tcggtcaggg ttacgtcaac ttcggtgaac caatgccgtt
    22501 gatgacctac cttaaccagc acgtaccaga ctggcgtgaa tctatcgatc ccatcgaagc
    22561 ggtgcgtccg gcctggttaa cgccgacggt caataatatt gctgccgatc tgatggtacg
    22621 cattaacaac gcaggcgcgg caaacgccat gaacctgtgt tgtactgcgc ttctggcatc
    22681 acgtcagcgc tcactcaccc gcgagcagtt aaccgagcaa ctcaactgct acctggatct
    22741 gatgcgcaac gtaccttact ccacggactc taccgttcct tcagccagcg ccagcgagct
    22801 tatcgatcac gcgctgcaaa tgaacaagtt tgaagtcgag aaagacacta tcggcgacat
    22861 catcattctg ccgcgcgagc aagcggtgct gatgacctac tatcgcaaca acattgcgca
    22921 tatgttggtg ctgccttcgc tgatggcggc aattgtcacc cagcatcgcc acatctcccg
    22981 cgacgtattg atggagcacg tcaatgtgct ttacccaatg ctgaaagcgg agctgttcct
    23041 gcgctgggat cgcgacgagt tgccggacgt tattgatgcg ctggcaaatg agatgcaacg
    23101 tcaggggctg attaccctgc aagatgatga gttgcatatc aacccggcgc attctcgccc
    23161 cctacagctg ctggccgcag gcgcgcgcga aacgctgcaa cgttatgcca tcaccttctg
    23221 gttgttgagt gccaacccgt cgatcaaccg cggtacgctg gagaaagaga gccgcaccgt
    23281 tgcgcaacgt ctctccgtgc tgcacggcat caacgcgccg gagttcttcg acaaggcggt
    23341 gttcagttct ctggtgctga cgctgcgtga tgaagggtat atcagcgata gcggcgacgc
    23401 cgaaccggca gaaacgatga aggtttatca gttgctggcg gagttgatta catcagacgt
    23461 gcgtttgacg attgagagtg cgacgcaggg cgaagggtaa tcagagagaa ttgccggatg
    23521 cggcgaaaac gccttatccg gccttccata atctgcaaat tcaataaatt gcgatgtgtt
    23581 gtgtaggcct gagaagcgca gcgcatcagg caacccagaa gaaagccgga tgatcatccg
    23641 gcttttttac atcatcagaa atgccagtaa ctcattgcca gtcctaaaaa cagtaccaga
    23701 ccaacatagt tattattcat aaatgcttta aagcaggctt cacgctcgcg gttggcaatc
    23761 agtttttgtt gataaacaaa cagcgcgcca gccaccagaa ttgaccagta atatccccag
    23821 cctaagccat ttaactcacc gatgatcgcc atcagtgcca gtacgccaat ctgcaaaata
    23881 ccaataatca atttatcgta ttggccgaac aggattgcgg tggatttaat accaatcttc
    23941 acatcatcgt cacggtcaac cattgcatac tgcgtgtcgt aagccaccgc ccagagaata
    24001 ttggcgagga acattaacca gcaactcaat ggcaccgact cactcacagc ggcaaaagcc
    24061 attggaatcg accagccaaa cgccgcgccc agcaccactt gcgggagatg ggtataccgc
    24121 ttcataaacg ggtacaccca cgccaacgcc agagcggcaa tcgacaacag aatggtcatc
    24181 gtattcagcg tcagcaccag taaaaatgaa atcaatacca gcactacaaa cagcgcgcgc
    24241 gcctctttct ccgttaccgc gccgctggga agcggtcggt tcgccgtgcg cttaacatga
    24301 ccatcaaact tgcggtcagc ataatcattc accacacatc cggcagcgcg catcagccag
    24361 acacccgcga caaacaccgc caggatccag agctggggaa cgcccggtgt cgccacccac
    24421 aacgcccata atgttggcca gagcagcagt aacgcgccaa ttggcttatc cgtacgcatt
    24481 aagcgatgaa acgccagcag cttattctgc gtcagactcc actccatatt tttttcctct
    24541 tagtacaacg gtgacgccgg taaaaacagt tctgttagca acagcggttt accgcttaat
    24601 cgcaggcggg aacgtcgccc ccacagtccg gcatcacggc ctatctcaat aaagtcccgg
    24661 gttaatgtcg atgatgtgaa cagatagcgt cctagcggcg ttttacccaa tttttgtaac
    24721 gccagctccg gcccgcttaa cgttgacaca ggaacgacgg tacgaccggc aagccacggt
    24781 tcaccatcgg cacataacaa aatttcacgt aaccagtaac gagactcttt cggcagcagc
    24841 ggcagttctt cggggatttc attctgctcg acaaaccctt cgcggatcat cgtcacgctc
    24901 accgtttttc cctgctgttc aaaacgtttt gtcatggaat cttccagcaa cagccagtcg
    24961 agcagttgcg aatccagggc agggatctct ttaaaatagc gcagcgcacg cagttgcgtt
    25021 aacgcggggt gtgacatgcc gaactctccg ttacataacg taaaggcatt gtatcgcaga
    25081 aaagtgttta cgggagatgt aaagcaaatt aagtgatcta atgcgcaaca aaaacgcaac
    25141 aatcgcatga agtggtaaaa cgctgttgaa ccttacgctt tgccgatgcc cgtgctggcg
    25201 agggcaaccg taggggcatc accgcccatg atgccaggaa cggtgatgtt ttcctttact
    25261 taaataccta tattctttat acgagtagct cgccaactcg cagtaaaaat tgggaaaagc
    25321 gcatatttac catgagattt ggagctaaaa agtgtttcct gagtaagatt aataggcaac
    25381 atcttatgtc tggatttata gatacacgtg gaattccaat tggtatcttt tgtaggattc
    25441 aacatatca
//
LOCUS       DAQSWQ010000054        25284 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.054, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000054 DAQSWQ010000000
VERSION     DAQSWQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.054"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            17..268
                     /locus_tag="R8G18_002342"
                     /pseudo
     CDS             17..268
                     /locus_tag="R8G18_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="R8G18_002343"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="R8G18_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEE9796018.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="R8G18_002344"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="R8G18_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEE9796019.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="R8G18_002345"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="R8G18_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEE9796020.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="R8G18_002346"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="R8G18_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEE9796021.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="R8G18_002347"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="R8G18_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEE9796022.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="R8G18_002348"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="R8G18_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEE9796023.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="R8G18_002349"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="R8G18_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEE9796024.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="R8G18_002350"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="R8G18_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEE9796025.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="R8G18_002351"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="R8G18_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEE9796026.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="R8G18_002352"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="R8G18_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEE9796027.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="R8G18_002353"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="R8G18_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEE9796028.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="R8G18_002354"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="R8G18_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEE9796029.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="R8G18_002355"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="R8G18_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEE9796030.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="R8G18_002356"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="R8G18_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEE9796031.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="R8G18_002357"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="R8G18_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEE9796032.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="R8G18_002358"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="R8G18_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEE9796033.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="R8G18_002359"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="R8G18_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEE9796034.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="R8G18_002360"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="R8G18_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEE9796035.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="R8G18_002361"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="R8G18_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEE9796036.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="R8G18_002362"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="R8G18_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEE9796037.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="R8G18_002363"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="R8G18_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEE9796038.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="R8G18_002364"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="R8G18_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEE9796039.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="R8G18_002365"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="R8G18_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEE9796040.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="R8G18_002366"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="R8G18_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEE9796041.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="R8G18_002367"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="R8G18_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEE9796042.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="R8G18_002368"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="R8G18_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEE9796043.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="R8G18_002369"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="R8G18_002369"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEE9796044.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="R8G18_002370"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="R8G18_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEE9796045.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="R8G18_002371"
     CDS             14267..14635
                     /locus_tag="R8G18_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEE9796046.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="R8G18_002372"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="R8G18_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HEE9796047.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="R8G18_002373"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="R8G18_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HEE9796048.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="R8G18_002374"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="R8G18_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEE9796049.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="R8G18_002375"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="R8G18_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEE9796050.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="R8G18_002376"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="R8G18_002376"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEE9796051.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="R8G18_002377"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="R8G18_002377"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEE9796052.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="R8G18_002378"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="R8G18_002378"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEE9796053.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="R8G18_002379"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="R8G18_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEE9796054.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /locus_tag="R8G18_002380"
     CDS             21312..21785
                     /locus_tag="R8G18_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEE9796055.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="R8G18_002381"
     CDS             21814..22356
                     /locus_tag="R8G18_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEE9796056.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="R8G18_002382"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="R8G18_002382"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HEE9796057.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="R8G18_002383"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="R8G18_002383"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEE9796058.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="R8G18_002384"
     CDS             23753..24010
                     /locus_tag="R8G18_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HEE9796059.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="R8G18_002385"
     CDS             complement(23986..24540)
                     /locus_tag="R8G18_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEE9796060.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25013..>25284
                     /locus_tag="R8G18_002386"
     rRNA            25013..>25284
                     /locus_tag="R8G18_002386"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6442 a         5993 c         6617 g         6232 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    24901 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
    24961 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    25021 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    25081 gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg acgggtgagt aatgtctggg
    25141 aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc ataatgtcgc
    25201 aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga tgggattagc
    25261 ttgttggtgg ggtaacggct cacc
//
LOCUS       DAQSWQ010000055        25106 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.055, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000055 DAQSWQ010000000
VERSION     DAQSWQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.055"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            196..384
                     /locus_tag="R8G18_002387"
     CDS             196..384
                     /locus_tag="R8G18_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HEE9796061.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="R8G18_002388"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="R8G18_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HEE9796062.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="R8G18_002389"
     CDS             1145..1309
                     /locus_tag="R8G18_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HEE9796063.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1502..3763
                     /gene="bcsA"
                     /locus_tag="R8G18_002390"
     CDS             1502..3763
                     /gene="bcsA"
                     /locus_tag="R8G18_002390"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HEE9796064.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="R8G18_002391"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="R8G18_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HEE9796065.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="R8G18_002392"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="R8G18_002392"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HEE9796066.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="R8G18_002393"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="R8G18_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="R8G18_002394"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="R8G18_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HEE9796067.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="R8G18_002395"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="R8G18_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HEE9796068.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="R8G18_002396"
     CDS             14459..15955
                     /locus_tag="R8G18_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEE9796069.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="R8G18_002397"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="R8G18_002397"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HEE9796070.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="R8G18_002398"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="R8G18_002398"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HEE9796071.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="R8G18_002399"
     CDS             18049..20109
                     /locus_tag="R8G18_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEE9796072.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="R8G18_002400"
     CDS             complement(20291..21613)
                     /locus_tag="R8G18_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HEE9796073.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="R8G18_002401"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="R8G18_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HEE9796074.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="R8G18_002402"
     CDS             complement(23086..23985)
                     /locus_tag="R8G18_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9796075.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>25106
                     /locus_tag="R8G18_002403"
     CDS             24505..>25106
                     /locus_tag="R8G18_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEE9796076.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5895 a         6365 c         6992 g         5854 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctcag acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctggacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggca acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacag ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    25021 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    25081 gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAQSWQ010000056        25064 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.056, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000056 DAQSWQ010000000
VERSION     DAQSWQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.056"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(166..576)
                     /locus_tag="R8G18_002404"
     CDS             complement(166..576)
                     /locus_tag="R8G18_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HEE9796077.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNIRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="R8G18_002405"
     CDS             complement(634..867)
                     /locus_tag="R8G18_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HEE9796078.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="R8G18_002406"
     CDS             1256..1801
                     /locus_tag="R8G18_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HEE9796079.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="R8G18_002407"
     CDS             1776..3701
                     /locus_tag="R8G18_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEE9796080.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="R8G18_002408"
     CDS             3698..3904
                     /locus_tag="R8G18_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HEE9796081.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="R8G18_002409"
     CDS             3901..5502
                     /locus_tag="R8G18_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEE9796082.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="R8G18_002410"
     CDS             5483..6802
                     /locus_tag="R8G18_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEE9796083.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="R8G18_002411"
     CDS             6812..7144
                     /locus_tag="R8G18_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEE9796084.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="R8G18_002412"
     CDS             7200..8225
                     /locus_tag="R8G18_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEE9796085.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="R8G18_002413"
     CDS             8267..8665
                     /locus_tag="R8G18_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HEE9796086.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="R8G18_002414"
     CDS             8677..9030
                     /locus_tag="R8G18_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HEE9796087.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="R8G18_002415"
     CDS             9042..9620
                     /locus_tag="R8G18_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9796088.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="R8G18_002416"
     CDS             9617..10012
                     /locus_tag="R8G18_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9796089.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="R8G18_002417"
     CDS             10020..10760
                     /locus_tag="R8G18_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9796090.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="R8G18_002418"
     CDS             10776..11198
                     /locus_tag="R8G18_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HEE9796091.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="R8G18_002419"
     CDS             11180..11614
                     /locus_tag="R8G18_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HEE9796092.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="R8G18_002420"
     CDS             11607..14168
                     /locus_tag="R8G18_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEE9796093.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="R8G18_002421"
     CDS             14165..14494
                     /locus_tag="R8G18_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9796094.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="R8G18_002422"
     CDS             14494..15192
                     /locus_tag="R8G18_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HEE9796095.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="R8G18_002423"
     CDS             15198..15941
                     /locus_tag="R8G18_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9796096.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15878..16510
                     /locus_tag="R8G18_002424"
     CDS             15878..16510
                     /locus_tag="R8G18_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HEE9796097.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     IYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20068
                     /locus_tag="R8G18_002425"
     CDS             16571..20068
                     /locus_tag="R8G18_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HEE9796098.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20139..20655
                     /locus_tag="R8G18_002426"
                     /pseudo
     CDS             20139..20655
                     /locus_tag="R8G18_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20720..22124
                     /locus_tag="R8G18_002427"
                     /pseudo
     CDS             20720..22124
                     /locus_tag="R8G18_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            22073..22225
                     /locus_tag="R8G18_002428"
                     /pseudo
     CDS             22073..22225
                     /locus_tag="R8G18_002428"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            22202..22381
                     /locus_tag="R8G18_002429"
                     /pseudo
     CDS             22202..22381
                     /locus_tag="R8G18_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23096..23803
                     /locus_tag="R8G18_002430"
                     /pseudo
     CDS             23096..23803
                     /locus_tag="R8G18_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23803..24319
                     /locus_tag="R8G18_002431"
                     /pseudo
     CDS             23803..24319
                     /locus_tag="R8G18_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         6024 a         6185 c         7539 g         5316 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttatatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagctggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgcggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgcct
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctctgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gcgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcgcagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacatc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa
    18961 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt
    19021 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
    19081 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
    19141 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
    19201 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
    19261 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
    19321 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
    19381 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaacgc ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct
    19501 gactccggac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
    19561 cgccgggacg ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga
    19621 ggcgactcag gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc
    19681 tggtacgtgg ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga
    19741 tcataacttt gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga
    19801 cgatccgggg agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt
    19861 tcgcaaaaac ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta
    19921 cagtgcagtt attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt
    19981 ccagaaaggc aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa
    20041 agcggcttcc ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga
    20101 atgacgcctt ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca
    20161 ttttgtccgc agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc
    20221 agtccacgct gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc
    20281 tgaacgggat taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt
    20341 cattcagcta tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc
    20401 atgaagattc cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga
    20461 atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt
    20521 ccggggatta tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa
    20581 gtgatgacgg tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc
    20641 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
    20701 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
    20761 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt
    20821 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
    20881 gtacggtcag tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac
    20941 catcaccgtg tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac
    21001 ggaggatgat gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
    21061 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc
    21121 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc
    21181 agccagcacg tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg
    21241 aacggcatca acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa
    21301 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc
    21361 acaacaatca gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac
    21421 ctcagcccgg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc
    21481 atcaagcgcc agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc
    21541 caaaacgtcc gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc
    21601 tgcggcaggc tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg
    21661 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc
    21721 ttcaacagcc acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc
    21781 agcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc
    21841 ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc
    21901 tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc
    21961 atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac
    22021 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
    22081 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc
    22141 gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga
    22201 cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca
    22261 gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa
    22321 agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc
    22381 tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag
    22441 cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc
    22501 atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat
    22561 tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat
    22621 aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac
    22681 caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga
    22741 ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac
    22801 agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac
    22861 gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta
    22921 tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg
    22981 ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac
    23041 cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt
    23101 ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
    23161 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc
    23221 aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc
    23281 cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag
    23341 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
    23401 caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca
    23461 tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg
    23521 gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac
    23581 ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac
    23641 agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt
    23701 tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
    23761 cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt
    23821 gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt
    23881 gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacatcgt ggcatctcgt
    23941 tgaagatcat cggggtaaaa cggtttatga cgtggcttcc ggcgacgcgt tatttatttc
    24001 tgaactcggc tcattaccgg aaaatgtcac ctggttatcc ccggaagggg agtttcagaa
    24061 gtggaacggc acagcctggg tgaaggatac ggaagcagaa aaactgttcc ggatccggga
    24121 ggcggaagaa acaaaaaaca gcctgatgca ggtagccagt gagcatatag cgccgcttca
    24181 ggatgctgtg gatctggaga tcgcaacgga gaaagaaacc ttgttgctgg aagcctggaa
    24241 aaagtatcgg gtgttgctga accgtgttga tacatcaact gcaccggata ttgaatggcc
    24301 agtagcacct atagggtaaa ttcgtaatga ttacctaaat acgttattct tttgttaaaa
    24361 aagtgatttt gctataggta gggagtatgt tcttggatta agaataaaaa atcgccatga
    24421 aagagcttca tggcgagtac ataaacgtac aaaatgtgat gaaagtagag tggcgtaagc
    24481 cactatatta agatatgaca atagcgaaga aaagtaaata acattttatg gacgtttaat
    24541 tgttaattat aaaaactaga gtgcccgata ttgtttatct tgctttaaaa taatgtactc
    24601 aaaccaaatt tttgagctaa aagctcaaat aatataaatg caataataat gtttaataag
    24661 acaattagct tgctttgtat tttcatttcg gaatactgtt aagtaacttt gatgacccta
    24721 acaatagatc ggaattttct tagagtcaat ggtcactgtt tttgcttgca aagcaaatgt
    24781 tttggtggtt ataagttgtc ttaatatagt cacgcatgca acttgttcca tgcggagatt
    24841 aaagattgga atataacgcg aagatggaat taaattacat ttgcaataat caatttatta
    24901 ttactcaata attaccgata ttattattgt aaattaatca ttagagtgca aaaagaaatg
    24961 taacggcaaa ataagaggac gatagctgag aaaaaagcgt gctttcccaa tctggggcaa
    25021 atcacgtctg aatgacacct gaaaacaggt ggtagcctca agaa
//
LOCUS       DAQSWQ010000057        24934 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.057, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000057 DAQSWQ010000000
VERSION     DAQSWQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.057"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="R8G18_002432"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="R8G18_002432"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HEE9796099.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="R8G18_002433"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="R8G18_002433"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HEE9796100.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="R8G18_002434"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="R8G18_002434"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HEE9796101.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="R8G18_002435"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="R8G18_002435"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HEE9796102.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="R8G18_002436"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="R8G18_002436"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HEE9796103.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="R8G18_002437"
                     /pseudo
     CDS             5058..6191
                     /locus_tag="R8G18_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="R8G18_002438"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="R8G18_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HEE9796104.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="R8G18_002439"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="R8G18_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEE9796105.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="R8G18_002440"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="R8G18_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HEE9796106.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="R8G18_002441"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="R8G18_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HEE9796107.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="R8G18_002442"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="R8G18_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEE9796108.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="R8G18_002443"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="R8G18_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HEE9796109.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..10631
                     /locus_tag="R8G18_002444"
     CDS             9762..10631
                     /locus_tag="R8G18_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HEE9796110.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10834..11187)
                     /locus_tag="R8G18_002445"
     CDS             complement(10834..11187)
                     /locus_tag="R8G18_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HEE9796111.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11325..12971)
                     /gene="groL"
                     /locus_tag="R8G18_002446"
     CDS             complement(11325..12971)
                     /gene="groL"
                     /locus_tag="R8G18_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEE9796112.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13015..13308)
                     /locus_tag="R8G18_002447"
     CDS             complement(13015..13308)
                     /locus_tag="R8G18_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEE9796113.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13584..14840
                     /gene="yjeH"
                     /locus_tag="R8G18_002448"
     CDS             13584..14840
                     /gene="yjeH"
                     /locus_tag="R8G18_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HEE9796114.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14862..15332)
                     /gene="fxsA"
                     /locus_tag="R8G18_002449"
     CDS             complement(14862..15332)
                     /gene="fxsA"
                     /locus_tag="R8G18_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEE9796115.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15669..17105
                     /gene="aspA"
                     /locus_tag="R8G18_002450"
     CDS             15669..17105
                     /gene="aspA"
                     /locus_tag="R8G18_002450"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEE9796116.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17223..18524
                     /gene="dcuA"
                     /locus_tag="R8G18_002451"
     CDS             17223..18524
                     /gene="dcuA"
                     /locus_tag="R8G18_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HEE9796117.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18640..18978
                     /gene="cutA"
                     /locus_tag="R8G18_002452"
     CDS             18640..18978
                     /gene="cutA"
                     /locus_tag="R8G18_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HEE9796118.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18954..20651
                     /gene="dsbD"
                     /locus_tag="R8G18_002453"
     CDS             18954..20651
                     /gene="dsbD"
                     /locus_tag="R8G18_002453"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEE9796119.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20688..21263
                     /locus_tag="R8G18_002454"
     CDS             20688..21263
                     /locus_tag="R8G18_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEE9796120.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21370..21445
                     /locus_tag="R8G18_002455"
     tRNA            21370..21445
                     /locus_tag="R8G18_002455"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21403..21405,aa:Phe,seq:gaa)
     gene            22062..23599
                     /gene="cadC"
                     /locus_tag="R8G18_002456"
                     /pseudo
     CDS             22062..23599
                     /gene="cadC"
                     /locus_tag="R8G18_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            23964..>24934
                     /gene="cadB"
                     /locus_tag="R8G18_002457"
     CDS             23964..>24934
                     /gene="cadB"
                     /locus_tag="R8G18_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HEE9796121.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYNANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         6223 a         6265 c         6122 g         6324 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tctagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc
     9901 tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc
     9961 agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc
    10021 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
    10081 ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt
    10141 tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata
    10201 atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat
    10261 acgacgttga ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat
    10321 ggaaacataa cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
    10381 gcaaagaaca atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa
    10441 tagaggcgat cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg
    10501 tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg
    10561 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
    10621 aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg
    10681 ataacgtgat cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc
    10741 tcaagcattc aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca
    10801 ataatacgtt tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc
    10861 gataatctgg ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat
    10921 gccataaatc acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc
    10981 gcgcatagaa ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt
    11041 cgcagtacca atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg
    11101 accggcagca ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac
    11161 aatccctgct aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac
    11221 tcgcgtcgtc cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac
    11281 atttctgccc gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc
    11341 atgccaccca tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc
    11401 aggtcggtaa ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc
    11461 agagcagaac gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat
    11521 tcttcggttg ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca
    11581 acagacggtt cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc
    11641 agtgcaactt tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta
    11701 gacgctacgc ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca
    11761 cgggtcgcgt gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta
    11821 gcagcaccta ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt
    11881 ttttcacggt cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg
    11941 ccctggattg cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc
    12001 acaacacgtt tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc
    12061 tcttcagaga tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga
    12121 cgatcgccga agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta
    12181 acaaccagag ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg
    12241 cctgctttgg caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg
    12301 tcagccagca ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg
    12361 aagtaaggag acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc
    12421 tgcagaccgg taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct
    12481 tcagcgatca gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca
    12541 atcgctttag agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta
    12601 acggctttgt cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc
    12661 agaccctcag tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca
    12721 gcgtcgttcg ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg
    12781 tcttccagtt cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg
    12841 aaagatttat ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct
    12901 gccagtacgt ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct
    12961 ttagctgcca ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc
    13021 ttcaacaatt gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt
    13081 ctcagatttc acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag
    13141 cggcttcact tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg
    13201 ggtggattta gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac
    13261 ttctttacgc ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc
    13321 tttgagaaag tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt
    13381 gaccagagaa atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg
    13441 atcagcacaa aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat
    13501 cgccgtcata agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt
    13561 tgggcgcaat cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg
    13621 gcattggcct gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag
    13681 ctgcacaggt agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt
    13741 tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg
    13801 cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt
    13861 tatcggtcat tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg
    13921 caatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg
    13981 tgtggtatat cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg
    14041 gacttatcgt cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata
    14101 tccccttccc ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga
    14161 tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc
    14221 cagagcgtga ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact
    14281 ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag
    14341 catcgcttcc aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg
    14401 taattggcta tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc
    14461 tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc
    14521 atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga
    14581 ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta
    14641 tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac
    14701 tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc
    14761 tctatgcgct gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg
    14821 aaaatggcat aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga
    14881 tcaaggcggt cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca
    14941 ccggtcccgg cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac
    15001 ttcactgtca gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg
    15061 aaaaagcccg gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc
    15121 tccgccgcag ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag
    15181 ccctggttac gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc
    15241 agcaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa
    15301 aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta
    15361 aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa
    15421 atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac
    15481 aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc
    15541 ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa
    15601 acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga
    15661 aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc
    15721 cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca
    15781 acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg
    15841 cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg
    15901 ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct
    15961 accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg
    16021 gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg
    16081 ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact
    16141 cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag
    16201 ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga
    16261 tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca
    16321 tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg
    16381 gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg
    16441 gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg
    16501 ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc
    16561 tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc
    16621 aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc
    16681 aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc
    16741 agctgcagtt gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca
    16801 ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca
    16861 aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt
    16921 tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg
    16981 tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct
    17041 ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac
    17101 agtaatcgta cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt
    17161 gtgagcagta acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta
    17221 atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg
    17281 ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg
    17341 ttaaacccgg taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta
    17401 tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc
    17461 tgcgccgtaa cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa
    17521 tctttgctgg tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga
    17581 aggaacaagg cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg
    17641 cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc
    17701 atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc
    17761 tggtgatgtc cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt
    17821 atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca
    17881 aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct
    17941 atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca
    18001 acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg
    18061 ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta
    18121 tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag
    18181 ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg
    18241 cttctgctct gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg
    18301 cactgaacgt ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca
    18361 ttctgccgac ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc
    18421 gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc
    18481 tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgacca atcgcggggc
    18541 gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga
    18601 cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg
    18661 aataccgcgt ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta
    18721 gccgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc
    18781 tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa
    18841 actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa
    18901 accccggaac ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc
    18961 aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg
    19021 acgcgccggg acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc
    19081 aaaaccaaca tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta
    19141 aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg
    19201 tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc
    19261 ccgtaaccat caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg
    19321 ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg
    19381 ccaacaacgc agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat
    19441 tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg
    19501 tgctgccaat gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca
    19561 ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg
    19621 cgctgggtct ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat
    19681 acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt
    19741 ttactctaca actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac
    19801 agggcggctc acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac
    19861 catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt
    19921 ggctgggcgg cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa
    19981 ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa
    20041 ccgcgtttgg ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg
    20101 atatatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta
    20161 tcaccagcct acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag
    20221 cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc
    20281 aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc
    20341 tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct
    20401 gtaaaaagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca
    20461 cggtcttact tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc
    20521 atcttaatgt ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc
    20581 cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc
    20641 gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag
    20701 atgtactggg agaagccctg aaattattag aattacaagg gattgccaac accacgctgg
    20761 agatggttgc tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca
    20821 aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc
    20881 gtcagctgat gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg
    20941 cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta
    21001 cgttttatcc cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg
    21061 cctacgattt cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag
    21121 caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc
    21181 aggcggatgt cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc
    21241 gccagggtgg tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta
    21301 agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg
    21361 cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt
    21421 ggttcgattc cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt
    21481 ttcgtttcag gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa
    21541 ccagattctc cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt
    21601 cctgagcgat agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt
    21661 caaatgacgt catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga
    21721 ctgatgagat tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa
    21781 gactaacaat ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac
    21841 acatttttct ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc
    21901 agtaaaatcc tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc
    21961 ttgcattcac caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc
    22021 aattatcatt gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc
    22081 gcgttggcga atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac
    22141 ttacccttga gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag
    22201 tacttagcag ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg
    22261 ttgtgacgca gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc
    22321 ctgtctatat cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt
    22381 acagcgaaga agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg
    22441 ttcctgacac aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg
    22501 aacaatcccc agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt
    22561 gtcgttaggt atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat
    22621 gagcaaatcg cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag
    22681 ttgtaacagc tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt
    22741 ggcgacatca cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat
    22801 tgatgaaccg agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata
    22861 ccgtgctcaa caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat
    22921 gctggataaa cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact
    22981 ggagagttta tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca
    23041 gcaaattttg cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt
    23101 actgcatggc gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca
    23161 atcatcccca gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca
    23221 ttctcaacac cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat
    23281 tgttacactg ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc
    23341 tctggtaaaa ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga
    23401 aatgtcctgg ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg
    23461 ggaagcagct gatgcatatc tcactgcctt taatttacgc ccaggggcaa acacccttta
    23521 ctggattgaa aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa
    23581 atttctcgct tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg
    23641 agtttttgta tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt
    23701 tattacataa atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc
    23761 ttttcatgat atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta
    23821 cagaaacttt tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc
    23881 ttatcgccag tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc
    23941 aaaaatgaaa ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta
    24001 ccggtgttgt tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag
    24061 caagtatcgg tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc
    24121 tggcgtatgt atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt
    24181 atgccggaga aatttctcct gcatttggtt ttcagacagg tgttctttat tacaatgcta
    24241 actggattgg taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc
    24301 cagtattaaa tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta
    24361 cctttgtaaa tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc
    24421 tggttcttat tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa
    24481 cttatgcagc taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta
    24541 ttctgctcta cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg
    24601 ttaaaaaccc gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta
    24661 ttgtttacat cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg
    24721 cttccggtgc tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc
    24781 tggtttctgc attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg
    24841 taggccaggc aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag
    24901 tcgacagcaa cggtattccg aaaaaaggtc tgct
//
LOCUS       DAQSWQ010000058        23951 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.058, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000058 DAQSWQ010000000
VERSION     DAQSWQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.058"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(38..400)
                     /locus_tag="R8G18_002458"
     CDS             complement(38..400)
                     /locus_tag="R8G18_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HEE9796122.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="R8G18_002459"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="R8G18_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEE9796123.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="R8G18_002460"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="R8G18_002460"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEE9796124.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="R8G18_002461"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="R8G18_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HEE9796125.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="R8G18_002462"
     CDS             complement(4827..5036)
                     /locus_tag="R8G18_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796126.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="R8G18_002463"
     CDS             5321..5683
                     /locus_tag="R8G18_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HEE9796127.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6226..6904
                     /locus_tag="R8G18_002464"
                     /pseudo
     CDS             6226..6904
                     /locus_tag="R8G18_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6948..11798
                     /locus_tag="R8G18_002465"
     CDS             6948..11798
                     /locus_tag="R8G18_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEE9796128.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKTLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12166..13821
                     /gene="lldP"
                     /locus_tag="R8G18_002466"
     CDS             12166..13821
                     /gene="lldP"
                     /locus_tag="R8G18_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEE9796129.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13821..14597
                     /gene="lldR"
                     /locus_tag="R8G18_002467"
     CDS             13821..14597
                     /gene="lldR"
                     /locus_tag="R8G18_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HEE9796130.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14594..15784
                     /gene="lldD"
                     /locus_tag="R8G18_002468"
     CDS             14594..15784
                     /gene="lldD"
                     /locus_tag="R8G18_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HEE9796131.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15980..16453
                     /gene="trmL"
                     /locus_tag="R8G18_002469"
     CDS             15980..16453
                     /gene="trmL"
                     /locus_tag="R8G18_002469"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEE9796132.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="R8G18_002470"
     CDS             complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="R8G18_002470"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEE9796133.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="R8G18_002471"
     CDS             complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="R8G18_002471"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEE9796134.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18426..18893)
                     /gene="secB"
                     /locus_tag="R8G18_002472"
     CDS             complement(18426..18893)
                     /gene="secB"
                     /locus_tag="R8G18_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEE9796135.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="R8G18_002473"
     CDS             complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="R8G18_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEE9796136.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19349..19780)
                     /locus_tag="R8G18_002474"
     CDS             complement(19349..19780)
                     /locus_tag="R8G18_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEE9796137.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20025..21569
                     /gene="gpmM"
                     /locus_tag="R8G18_002475"
                     /gene_synonym="pgmI"
     CDS             20025..21569
                     /gene="gpmM"
                     /locus_tag="R8G18_002475"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEE9796138.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21579..22862
                     /gene="envC"
                     /locus_tag="R8G18_002476"
     CDS             21579..22862
                     /gene="envC"
                     /locus_tag="R8G18_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HEE9796139.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22866..23825
                     /locus_tag="R8G18_002477"
     CDS             22866..23825
                     /locus_tag="R8G18_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEE9796140.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(23812..>23951)
                     /locus_tag="R8G18_002478"
     CDS             complement(23812..>23951)
                     /locus_tag="R8G18_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEE9796141.1"
                     /translation="YKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFNL
                     R"
BASE COUNT         6044 a         6109 c         6211 g         5587 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gatttaattg tgaaatttta
     5941 atcatcatat tcatacaata aattatctgt aaattttttc ccagacatgg tttctttcat
     6001 atttgacatt tattaataat catcattaga atccacgaaa ataatatatt catacaaaca
     6061 cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat
     6121 cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata caatggacaa
     6181 ctctaccggc attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag
     6241 atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt
     6301 gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacatta aaattgatct
     6361 acttgaaaaa caacaagcac agctaaagag tcaactggaa actattcaaa aacaacaaac
     6421 tggcataata agcagtacaa agactttaac tcacgtaatt aaatccgtta aagatcaaca
     6481 aaacactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa
     6541 tggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga atggcagtgt
     6601 tattcatatt tcactggtca acttattaag tacacctatc tcaaatattg gttttaatgc
     6661 gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact
     6721 tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa
     6781 tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat
     6841 ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa
     6901 ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat
     6961 ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa
     7021 gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa
     7081 tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggccag ggtgttgatt
     7141 acggtagtgg atcagctggc gacagctggg ttgctatagg caaaggggcg aaagcaaata
     7201 cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc
     7261 aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg
     7321 gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat
     7381 tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg
     7441 ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctggga aatgccacta
     7501 aagctactga gattatgagt attgctcttg gcgacaccgc caatgcgtca aaagcgtatt
     7561 caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta
     7621 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
     7681 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
     7741 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
     7801 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
     7861 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
     7921 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
     7981 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
     8041 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
     8101 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
     8161 tttatgccat tagcgactcg gtagcaaaaa cgcttggagg aggggctgca gtagatgttg
     8221 atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag
     8281 gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat
     8341 acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg
     8401 gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg
     8461 acgatgtcga agccaacacc gccaatatcg ctactaatac cagcaacatt gccacgaata
     8521 cagcaagtat tgccaccaat accaccaata tcaccaacct gacggattcc gttggtgacc
     8581 ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc
     8641 aggataccac cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg
     8701 atgctgttaa cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca
     8761 atatcgccaa taacacttcc aatattgcca ctaacaccac caacatctct aatctgactg
     8821 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca
     8881 cggcagctca tggcaccgag accaccagca aaatcaccaa cgttaaagat ggcgacctga
     8941 cgactggcag caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg
     9001 cgacgaatac caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg
     9061 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag
     9121 ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa
     9181 ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct
     9241 acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa
     9301 tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta
     9361 gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac
     9421 acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact
     9481 ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg
     9541 cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg acgtacacca
     9601 ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat accactcttg
     9661 acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg
     9721 gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca
     9781 gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc gctgatgcgc
     9841 tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact tacaccattg
     9901 cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat aacgccctgc
     9961 tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat gacggtaaag
    10021 ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc gatgcagtga
    10081 acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa attatcaatc
    10141 agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt atcaactacg
    10201 ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt gtcggcgcta
    10261 cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt ggtcagaaca
    10321 gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc agccgtgtga
    10381 ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt ggttatgaca
    10441 ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa taccgccaaa
    10501 tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc cagttgcaga
    10561 acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac tcaacggcag
    10621 aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact gtcgttaatg
    10681 gtaatgcagg tattggtatc ggtttgaaca ctctggttct agctgatgcg atcaacggca
    10741 ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg ggtaacggtt
    10801 ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac gcaccgcaga
    10861 actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc acaaacgtcg
    10921 cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc actgatgagc
    10981 gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact aatctggata
    11041 ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt agcaccaagt
    11101 acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat agcgttgcta
    11161 ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc ggttccgttg
    11221 cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt atcaccaacg
    11281 tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag
    11341 caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc aatatcaccc
    11401 tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc gtcaacaaca
    11461 acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag caatacaccg
    11521 atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg
    11581 gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg ggtgccagca
    11641 tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta ggtgtatcga
    11701 tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat agccagggtg
    11761 aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag ttagtgtgac
    11821 taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata aggcgccccg
    11881 ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac cagacatctc
    11941 cccccacaag aattggccct accaattctt cgcttatctg acctctggtt cacaattccc
    12001 caattaaaac tcacattaat attgccaata cataacattt agttaaccat tcattgtcat
    12061 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
    12121 caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa
    12181 caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc
    12241 ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
    12301 acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
    12361 ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg gatcattatt
    12421 gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg
    12481 tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc
    12541 ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg
    12601 ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat tgttaacacc
    12661 gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca agtaacaggc
    12721 atcgacagct ttgagattgg ccagatggtg gggcggcagc taccgtttat gaccattatc
    12781 gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaagga gacgtggcct
    12841 gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt
    12901 gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
    12961 ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc gtcaaagatt
    13021 gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc ctggacaccg
    13081 ttcctgttcc tgacagctac cgtaacactg tggagcatcc cgccgtttaa agccctgttc
    13141 gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
    13201 ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag
    13261 tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc
    13321 tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
    13381 gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc
    13441 ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc
    13501 tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc
    13561 ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg
    13621 gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct
    13681 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
    13741 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
    13801 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
    13861 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
    13921 agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag gcgctggcaa
    13981 aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc
    14041 gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg
    14101 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat
    14161 ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag
    14221 caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga
    14281 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
    14341 tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac
    14401 aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg
    14461 cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg
    14521 aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga
    14581 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
    14641 tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg
    14701 ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc
    14761 cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc
    14821 accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc
    14881 ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
    14941 cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt
    15001 tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt
    15061 ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa
    15121 tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
    15181 cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg
    15241 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
    15301 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
    15361 ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg
    15421 tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc
    15481 ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt
    15541 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
    15601 ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa
    15661 agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag attcgctggt
    15721 gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc
    15781 atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga ccgttcggcg
    15841 ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc tcgcccctcc
    15901 ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg ctatcctgcc
    15961 cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa ttccgccaaa
    16021 tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctgcata tcatcgaacc
    16081 gatgggattt gcctgggacg ataagcgcct gcgccgcgcg gggctggact atcacgagtt
    16141 taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa atccccagcg
    16201 cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct atcaggatgg
    16261 cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc ttgatgccct
    16321 gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca tgaatctgtc
    16381 caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt
    16441 gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg cacgatcaca
    16501 ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa atgctggtcc
    16561 atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg aacgccagcg
    16621 gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat cttcgcgccg
    16681 cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac accttcacga
    16741 attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac agattgcaga
    16801 atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg
    16861 agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa
    16921 acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa ccagtgaccg
    16981 atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt tgagtattta
    17041 tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt
    17101 tccgggtcag cggcgtaggc ttcttccacc acttcacgga tagcaatagc aggcataatt
    17161 ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac taccaaggtt ttcgtgcttg
    17221 agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag cgttctggct
    17281 tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc ttactccaca
    17341 cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa caaaggttcc
    17401 ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataacg tcaatgctgc
    17461 ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta ttggcatttc
    17521 tacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc cttccaccac
    17581 ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca tcatgccaaa
    17641 acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca gccccgccat
    17701 gcccataaag gtggcaggat cggcacccag cgccgcgcca agacgcgaca tttcagccag
    17761 cccacgggtg atcagtgccg tacgcgcatt cgcaccaaaa ccgataccgt cggacatccc
    17821 cgcgccaatg gcaatgacgt ttttcaccgc accgccaagc tgcacaccga tgaaatccgg
    17881 gttgctgtaa acgcggaaac ttttgccaca gtgcagcaat tgttggagat catcggcaaa
    17941 ggtctggtcg gtagaggcca acgaaatagc cgtcggtaaa cctgccgcca gttctttcgc
    18001 aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct cacgcgccac
    18061 gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca ccagacgcgc
    18121 atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat ggctgggtac
    18181 gacgacgaga atattacggc tggctgccag cgcagtggcg agatcgcttt caagatggag
    18241 cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt
    18301 tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc ttgccagggt
    18361 aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag cattacgttg
    18421 gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg caaatagttc
    18481 atgaacagcg catcgaagtt aatcggcgca aggttcagtt gcgggaatgt accgcgggat
    18541 accacgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca gtatgcgccc
    18601 aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc gccctgctga
    18661 acttcacaca ggaacgcggt ttcttcgccc agggaggccg ttacggtaac acgcagtacc
    18721 acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag tttaacttct
    18781 ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg
    18841 gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga catgtgtaga
    18901 aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc acacgttatt
    18961 tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccgccaa
    19021 tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt ttgatcatct
    19081 cttcacgctt ggcggcattg ccatcgatcg gcaactcctg aaaactcacg cccttgctgc
    19141 tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat
    19201 tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg gcggctgcaa
    19261 aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt gcagctaacg
    19321 gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc aagttttcgc
    19381 cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca gctttcgtca
    19441 gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata accggtttgt
    19501 ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacagattga
    19561 tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag
    19621 cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg
    19681 tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca
    19741 gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac
    19801 gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc
    19861 gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg
    19921 gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta
    19981 gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa
    20041 aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag caggataacg
    20101 ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc
    20161 tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag
    20221 taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg
    20281 ttgaaatcaa agatcgcgct ttctttgcta atccagtgct gactggcgcg gtagataaag
    20341 cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc ggcgtacaca
    20401 gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa
    20461 tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc
    20521 tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg
    20581 gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc
    20641 tgctgactct ggcgcagggt gagtttcagg ccgatactgc cgttgctggt ttgcaggcag
    20701 cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc
    20761 agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc
    20821 gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc gcgcgtaaga
    20881 aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg
    20941 ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca
    21001 aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg
    21061 gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg
    21121 ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg
    21181 ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag
    21241 gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat cactgcgtgg
    21301 aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg
    21361 gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca cacaccaacc
    21421 tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca ggcggcaaac
    21481 tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga
    21541 ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata
    21601 ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg
    21661 ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac
    21721 tcaaatctat tcaggccgat atcgccacaa aagagcgcgc ggtacgccaa aagcaacaac
    21781 aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc
    21841 gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt gatgagatga
    21901 acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac
    21961 aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt ctcagcggtg
    22021 aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac caggcgcgac
    22081 aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag cgtgctgaac
    22141 tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc caacaggcga
    22201 aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa tcttccatcc
    22261 agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg cgtaacagta
    22321 ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt gaggctcagg
    22381 cagttcgcga caggcagaaa gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa
    22441 gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt caggcattct
    22501 ggccagttcg tggccctacg ctgcatcgct atggcgagca gctacagggt gaattacgct
    22561 ggaaaggaat ggttatcggt gcctctgaag gtactgaagt taaagcaatt gccgacggtc
    22621 gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt gagcacggta
    22681 aaggcgatat gagtctttac ggctataatc aaagcgcgct ggtgagcgtt ggttcgcagg
    22741 ttcgtgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggc agaccttcac
    22801 tctatttcga aattcgccgc cagggtcagg ccgtcaatcc acagccgtgg ttgggaagat
    22861 aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct
    22921 cccctgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgaaca
    22981 acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac
    23041 cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg ttgattcatc
    23101 tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctgcgc ccggagatga
    23161 gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg
    23221 ggatcaataa ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga
    23281 tgcaggcgct ggagcgttat aatctttact tcctcgacag cgtaaccatt ggtaataccc
    23341 aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag gtgttcctcg
    23401 acgattcaca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt gacctagcgc
    23461 gtcgcaacgg atcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgttgc
    23521 aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga
    23581 atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac acaccgcgta
    23641 atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta
    23701 atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc
    23761 agcatcagtg gcaaggctgg ggcaaacagc ctgaagcggc gaagcttaac gctagcgtaa
    23821 attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa
    23881 gcggaaagac cacagtaacg cctgataacc cacctttacg ctgcgcacat tggtaatcag
    23941 gcgcttatac a
//
LOCUS       DAQSWQ010000059        23891 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.059, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000059 DAQSWQ010000000
VERSION     DAQSWQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.059"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="R8G18_002479"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="R8G18_002479"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEE9796142.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="R8G18_002480"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="R8G18_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HEE9796143.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="R8G18_002481"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="R8G18_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEE9796144.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="R8G18_002482"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="R8G18_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HEE9796145.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="R8G18_002483"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="R8G18_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HEE9796146.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="R8G18_002484"
     CDS             5280..5738
                     /locus_tag="R8G18_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEE9796147.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="R8G18_002485"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="R8G18_002485"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEE9796148.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="R8G18_002486"
     tRNA            6790..6874
                     /locus_tag="R8G18_002486"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="R8G18_002487"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="R8G18_002487"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="R8G18_002488"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="R8G18_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEE9796149.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="R8G18_002489"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="R8G18_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEE9796150.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="R8G18_002490"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="R8G18_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEE9796151.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="R8G18_002491"
     CDS             complement(10156..13899)
                     /locus_tag="R8G18_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEE9796152.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="R8G18_002492"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="R8G18_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HEE9796153.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="R8G18_002493"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="R8G18_002493"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HEE9796154.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="R8G18_002494"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="R8G18_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HEE9796155.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="R8G18_002495"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="R8G18_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HEE9796156.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="R8G18_002496"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="R8G18_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HEE9796157.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="R8G18_002497"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="R8G18_002497"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HEE9796158.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="R8G18_002498"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="R8G18_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HEE9796159.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="R8G18_002499"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="R8G18_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HEE9796160.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="R8G18_002500"
     CDS             23294..23401
                     /locus_tag="R8G18_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HEE9796161.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23830..>23891
                     /locus_tag="R8G18_002501"
     CDS             23830..>23891
                     /locus_tag="R8G18_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HEE9796162.1"
                     /translation="MTLAKFAMIFWHDLAAPILAG"
BASE COUNT         6002 a         6238 c         5767 g         5884 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctcttccaca agtaaatatt ggcaacccga
    23521 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
    23581 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgagt cgtagtatcc
    23641 acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggcttaatc cctagtggca
    23701 ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac aacagaacgg
    23761 gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc attacaacaa
    23821 ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct ggcagcaccg
    23881 atcctggcgg g
//
LOCUS       DAQSWQ010000060        23757 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.060, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000060 DAQSWQ010000000
VERSION     DAQSWQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.060"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(29..829)
                     /locus_tag="R8G18_002502"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="R8G18_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1396..1761
                     /locus_tag="R8G18_002503"
     CDS             1396..1761
                     /locus_tag="R8G18_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEE9796163.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1801..2457
                     /locus_tag="R8G18_002504"
     CDS             1801..2457
                     /locus_tag="R8G18_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEE9796164.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2524..3942
                     /gene="dcm"
                     /locus_tag="R8G18_002505"
     CDS             2524..3942
                     /gene="dcm"
                     /locus_tag="R8G18_002505"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HEE9796165.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3923..4393
                     /gene="vsr"
                     /locus_tag="R8G18_002506"
     CDS             3923..4393
                     /gene="vsr"
                     /locus_tag="R8G18_002506"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HEE9796166.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="R8G18_002507"
                     /pseudo
     CDS             complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="R8G18_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5476..6393
                     /locus_tag="R8G18_002508"
     CDS             5476..6393
                     /locus_tag="R8G18_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEE9796167.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6472..6654
                     /locus_tag="R8G18_002509"
     CDS             6472..6654
                     /locus_tag="R8G18_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HEE9796168.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6825..8519
                     /gene="yedQ"
                     /locus_tag="R8G18_002510"
     CDS             6825..8519
                     /gene="yedQ"
                     /locus_tag="R8G18_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HEE9796169.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8516..9331)
                     /locus_tag="R8G18_002511"
     CDS             complement(8516..9331)
                     /locus_tag="R8G18_002511"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HEE9796170.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="R8G18_002512"
     CDS             complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="R8G18_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HEE9796171.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            10019..10207
                     /gene="dsrB"
                     /locus_tag="R8G18_002513"
     CDS             10019..10207
                     /gene="dsrB"
                     /locus_tag="R8G18_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HEE9796172.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="R8G18_002514"
     CDS             complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="R8G18_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HEE9796173.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="R8G18_002515"
     CDS             complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="R8G18_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HEE9796174.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="R8G18_002516"
     CDS             complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="R8G18_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEE9796175.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="R8G18_002517"
     CDS             complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="R8G18_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEE9796176.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="R8G18_002518"
     CDS             complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="R8G18_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HEE9796177.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="R8G18_002519"
     CDS             complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="R8G18_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEE9796178.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="R8G18_002520"
     CDS             complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="R8G18_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEE9796179.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="R8G18_002521"
     CDS             complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="R8G18_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HEE9796180.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="R8G18_002522"
     CDS             complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="R8G18_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HEE9796181.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="R8G18_002523"
     CDS             complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="R8G18_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HEE9796182.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="R8G18_002524"
     CDS             complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="R8G18_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HEE9796183.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="R8G18_002525"
     CDS             complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="R8G18_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEE9796184.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="R8G18_002526"
     CDS             complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="R8G18_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEE9796185.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="R8G18_002527"
     CDS             complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="R8G18_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HEE9796186.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21825..22139
                     /gene="fliE"
                     /locus_tag="R8G18_002528"
     CDS             21825..22139
                     /gene="fliE"
                     /locus_tag="R8G18_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEE9796187.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22473..22805
                     /gene="emrE"
                     /locus_tag="R8G18_002529"
     CDS             22473..22805
                     /gene="emrE"
                     /locus_tag="R8G18_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HEE9796188.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22974..23525
                     /gene="ybcL"
                     /locus_tag="R8G18_002530"
     CDS             22974..23525
                     /gene="ybcL"
                     /locus_tag="R8G18_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HEE9796189.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23535..>23757
                     /locus_tag="R8G18_002531"
     CDS             23535..>23757
                     /locus_tag="R8G18_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEE9796190.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
BASE COUNT         5769 a         5954 c         6144 g         5890 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aatgtattta
      961 aaagatttca aaatgtttct aatctattta atataattca gaattatgaa ttctaaatat
     1021 aaacatccat gaaaattata aatgtatatc aatatgttat gagctgaatg gaaagatagg
     1081 gagtatcaat gattgttgcg ccaaacggca ggcgtgatca cgcgcacatt aatgaaggta
     1141 taaaaaaagc cggcatcatg ccggcccaaa aaatataaac aagacgataa aaggctaata
     1201 acggggttat catgacacag aaatcaaaaa aggtataatt gtattcagtg ctaatatatt
     1261 gtttgggtga attaataaaa ctattaccaa ggatataaat aaatctttat tgctgttttt
     1321 ccagtaatta ttgccgttgc gcaaaattta tctatttgtt caaaaaatga ttgagtcttg
     1381 actggctcat ccaatgtgga aaaatgtgac ttttatcaca taatagtact aagtctgaat
     1441 tttccgggtt atctcaaaat ggaatacggt tcgacaaaga tggaagagag actctctcgc
     1501 agccctggcg gcaaattggc tttgtgggct ttctatacat ggtgtggcta tttcgtctgg
     1561 gcaatggcgc gttacatctg ggtgatgagc cggatccctg atgccccggt gtcgggattt
     1621 gaaagcgatt taggctctac cgcgggaaag tggttagggg cactggtcgg atttttattt
     1681 atggctctgg tcggggcatt gttaggcagc attgcctggt acacccgacc tcgtcccgca
     1741 cgtagcagac gctatgaata aaaactgcta aggtggcgac tctttggcaa cggagcgcgt
     1801 atggacttac aacactggca ggcacagttc gaaaactggt tgaaaaacca tcatcagcac
     1861 caggacgcgg cacagaagct ggcggcagat gacgatgtcg atatgctggt gattttaacc
     1921 gcctgttatt ttcacgatat tgtgagcctg gcgaaaaatc atccgcaaag gcagcgttct
     1981 tcaatcctgg cggcagaaga gacgcgtcgc ctgctccgtg aagagttcga gcaatttccg
     2041 gcggagaaaa tcgaggccgt ttgtcatgcc attgccgctc acagtttcag cgcgcaaatc
     2101 gcccccttaa caacggaagc taaaatcgtc caggatgcgg atcggctgga agccctgggg
     2161 gcgattggcc tggcgcgcgt gtttgccgtt tcaggggcat tgggcgtggc gctgtttgat
     2221 ggtgaagatc cgttcgcaca gcatcgcccg cttgatgata aacgctacgc gctggatcat
     2281 ttccagacta agttgctgaa gctaccgcaa accatgcaaa ccgcaagagg caagcagctg
     2341 gcgcagcaca acgcgcattt tttagtcgag tttatggcga agctcagtgc cgaactggcg
     2401 ggggagaatg aaggtgtcga tcacaaggtg atagatgcgt tttcacccgc gggataagcg
     2461 cgtggcccta aatggctgta attatgttaa cctgtcggcc atctcagatg gccggttaaa
     2521 tctatgcagg aaaatatatc agtaaccgat tcatacagca ccgggaatgc cgcacaggca
     2581 atgctggaga aactgctgca aatttatgat gttaaaacgc tggtggcgca gcttaatggt
     2641 gtgggtgaga atcactggag cgcggcaatt ttaaaacgtg cgctggcgaa tgactcggca
     2701 tggcaccgtt taagtgagaa agagttcgcc catctgcaaa cgttgttacc caaaccatcg
     2761 gcacatcatc cgcattatgc gtttcgcttt atcgatctat ttgccggaat tggcggcatc
     2821 cgtcgcggtt ttgaatcgat tggcggacaa tgcgtgttta ccagcgaatg gaacaaacat
     2881 gcggtacgca cttataaagc caaccattat tgcgatccgg cgacgcatca ttttaatgaa
     2941 gatatccgcg acatcaccct cagccataaa gaaggcgtga gtgatgaggc ggcggcggaa
     3001 catattcgtc aacacattcc tgaacacgat gttttactgg ccggtttccc ttgtcagcca
     3061 ttttcgctgg ctggcgtatc gaaaaagaac tcgctcgggc gggcgcacgg ttttgcctgc
     3121 gatacccagg gcacgctgtt ttttgatgtg gtacgcatta tcgacgcgcg tcgtccggcg
     3181 atgtttgtgc tcgaaaacgt caaaaacctg aaaagtcacg accagggtaa aacgttccgc
     3241 atcatcatgc agacgctgga cgaactgggc tatgacgtgg ctgatgcaga agataacggg
     3301 ccggacgatc cgaaaatcat cgatggtaaa cattttctgc cgcagcaccg tgaacgcatc
     3361 gtgctggtgg gttttcgtcg cgatcttaat ctgaaagccg attttactct gcgtgatatc
     3421 agcgaatgtt tccctgcaca gcgagtgacg ctggcgcagc tgttggaccc gatggtcgag
     3481 gcgaaatata tcctgacgcc ggtgctgtgg aagtacctct atcgatatgc gaaaaaacat
     3541 caggcgcgcg gtaacggctt cggttatgga atggtttatc cgaacaatcc gcaaagcgtc
     3601 acgcgtacgc tgtctgcgcg ttattacaaa gatggcgcgg aaattttaat cgatcgcggc
     3661 tgggatatgg ccacgggtga gaaagatttt gacgatccgc tgaatcagca acatcgtcca
     3721 cgtcggttaa cgcctcggga atgcgcgcgc ttaatgggtt ttgaagcgcc gggagaagcg
     3781 aaattccgca ttccggtttc ggacactcag gcctatcgcc agttcggtaa ctcggtggtc
     3841 gtgccggtct ttgccgcggt ggcaaaactg cttgagccaa aaatcaaaca ggcggtggcg
     3901 ttgcgtcagc aagaggcaca acatggccga cgttcacgat aaggccactc gcagcaaaaa
     3961 tatgcgcgcg attgccacgc gtgatacggc gattgagaag cgcctcgcca gtctgttaac
     4021 cgggcagggc ctggcgtttc gcgttcagga cgccagtctg cccggaagcc cggattttgt
     4081 cgttgatgaa tatcgctgcg tgatatttac ccatggctgc ttctggcatc atcatcactg
     4141 ctatctgttt aaagtgcctg cgactcgaac cgagttctgg ctggagaaga taggtaaaaa
     4201 tgttgaacgc gatcgccgcg atatcagtcg tttgcaggaa ctcggctggc gcgtactgat
     4261 tgtctgggag tgtgcattac gtgggcgcga gaagctgacg gatgaagcgc ttaccgagcg
     4321 tctggaagag tggatctgcg gcgaaggtgc cagcgcgcag atcgacacgc aggggattca
     4381 tttactcgct tgatgcgtcc tgaataactg gcgcaactac gggttttgcc gggaagagat
     4441 attttcccaa cgtgaccagt accaccgcga agacaattac gccgagcgcc agccattcaa
     4501 tcttcgacag cgtctctccg cccagtcccg tacccagcaa gaccgcgacc accgggttaa
     4561 cgtaggcgta gctggtggcg agagccggac tgacattacg gattaaatac atataagcgt
     4621 tgatggcgat aatcgaacca aacagcgcca gatagccgac cgcaaggaag cctgaaaggg
     4681 aagggagcgc cgtcagtttt tcacctgcaa tcatcgacgc gatcattaac accacgcctg
     4741 ccgccagcat ctcaatcgca cccgccatca tccctacagg taaggtaatg cgcgagccat
     4801 aaaccgagcc aaacgcccag ctaatcgagc cgattaaaat cagaatcgcg ccccacggat
     4861 tgccgcttaa atttccaccg ctattgagca tgatgattcc ggcaagccca atggcgatac
     4921 ccacccattc cagtttgcgc gttttaatgc caaacaggcg gctgaagcac cagggtaaag
     4981 aggggcacgg ttgcaaccac aacggcggcg atgccggaag gaacattttg atgctcggca
     5041 accgtcacca tgccattacc gacagccagc aataacaggc caatcagcgc ggcattgagc
     5101 agcggacgta gcggggggag cttgtgtccg cgcagtagca aaaatgccat caataaaata
     5161 ccggctgcca ggaatcgaac gcctgccatc attaacggag gccagctttc cacgccaatc
     5221 cgaatgacaa aataggttga gccccaaatg atatacaacg caaacagcgc gccaaaaagc
     5281 ggtaacaact ggcggaaacg cataatccct cacggtggaa ataaaaaggt ggttcatagt
     5341 aaacgtgaaa atcattctgc tggcgagaga tataattgca cttgattgta aaaaaaatgt
     5401 tgacctgtga agcaagtatc agagctgccg tttttgcttc atacttacac ccttcaacaa
     5461 taaaaatgag agggaatgct tttggccggg agtagtttac tgacgttgct cgatgatatc
     5521 gccacactac tggacgatat ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt
     5581 gtattagggg atgacttatc gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg
     5641 gaacttcccg tggtctgggg cgtggcgaaa ggatcgctga ttaataaagt gattctggtg
     5701 ccgctggcgc tgatcatcag tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt
     5761 ggtggcgcgt ttctctgctt tgaaggagta gagaaagtgc tgcatatgct ggaggcgcgt
     5821 aaacataaag aagatccggc gcagagccag cagcgtctgg agaagctggc ggcgcaggat
     5881 ccgctgaagt ttgaaaagga caaaataaaa ggggcgattc gtaccgattt tatattgtct
     5941 gccgaaatcg tcgccatcac gctggggatt gtggccgaag cgccgttgct taatcaggtg
     6001 ctggtgcttt caggcatcgc gctggtagtg accgtgggcg tctacggtct ggtaggggtt
     6061 atcgttaaga ttgatgacct ggggtattgg ctggcggaaa aatccagcgc gctgatgcag
     6121 gcattaggta aagggttatt gatcattgcg ccctggttga tgaaggcgtt atcgattgtc
     6181 ggcacgctgg cgatgttcct cgtcggcggc ggtattgtgg tacatggtat tgcgccgctg
     6241 catcacgcca ttgaacattt cgccgggcag caaagtgcag tggtggcgat gatattaccg
     6301 actgttttaa atctgattct tggatttatc atcggcggca tcgtggtgct gggagtgaaa
     6361 gccgtagcga aaatgcgcgg tcaggcacat taatactcgt acaggctatt atgcaaaatt
     6421 cgccatcttc aactaaggtg aacaagtttc actcgaaaaa aggaggcgag tatgagcttt
     6481 atggttagtg aggaagttac ggttaaagag ggcggcccgc ggatgattgt cactggatac
     6541 tccagcggta tggttgagtg tcgttggtat gacggttacg gggtcaagcg ggaagctttt
     6601 catgaaaccg agcttgttcc gggggagggg agtcgttctg cggaagaagt ttgaaagggt
     6661 atcattaaca ccaggccgga tcacttccgg cctggtgtgc caatgcctgc aatgaatgat
     6721 agtcgcgtct tatcatgcca ccattataaa attatcgctg ttttgcggta cgctatcgcc
     6781 aggttatgcc agaatcataa aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa
     6841 aaatggaaaa ccagagctgg ttgaaaaaac tcgcacgccg cctggggcct ggtcatgtcg
     6901 ttaatctctg ctttatcgtg gtattgcttt tttccacctt gctgacctgg cgtgaagtgg
     6961 tagtgctgga agatgcctat atctccagcc agcgtaatca tctggaaaac gtagccaacg
     7021 cgctcgataa gcatttgcag tataacgtcg acaaactgat ctttttgcgt aatggcatgc
     7081 gcgaagctct cgtagcgcca ctggatttca cttcactgcg taatgctgta accgagttcg
     7141 aacagcatcg cgacgagcac gcctggcaaa ttgaactcaa ccgacgacgc accctgtcag
     7201 tcaatggcgt atcggatgca ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa
     7261 gcctcgacaa tgaaattacc gctgcactgg aagttggtta cttgctgcga ctggcgcaca
     7321 acacctcgtc gatggttgaa caggcgatgt atgtctcgcg tgccggattt tacgtttcga
     7381 cgcagccgac cttgtttacg cgcaatgtac caacgcgtta ttacggctat gtcacccaac
     7441 cctggtttat cggccattcg caacgagaaa atcgtcaccg cgcggtacgc tggtttactt
     7501 cgcaaccgga acacgccagc aatactgaac cgcaggttac cgtcagtgtt ccggtagaca
     7561 gtaataacta ctggtatggc gtgctgggga tgagtattcc cgtgcgtacc atgcagcaat
     7621 ttttaagaaa cgccatcgat aaaaacctcg atggtgagta tcagctctat gacagtaagc
     7681 tgagattttt gacctcttcc aatcctgacc atccaacagg gaatattttt gatcctcgtg
     7741 aactggcctt gctggcgcag gcgatggaac atgacacgcg gggcggcatt cgtatggaca
     7801 gtcgctatgt tagctgggaa cgtctggacc atttcgacgg tgtgctggtg cgcgtccata
     7861 cgctaagcga aggcgtgcgc ggcgatttcg gcagtatcag cattgcatta accctgctgt
     7921 gggcgctctt taccaccatg ttactcatct cctggtatgt gattcgccgg atggttagca
     7981 acatgtatgt tctgcaaagc tcgttgcagt ggcaggcgtg gcacgacacc ttaacccgtt
     8041 tatataatcg tggcgcactg tttgaaaaag cccgtccgct cgcgaaattg tgtcagacgc
     8101 accaacatcc tttttctgtc attcaggtcg accttgacca ttttaaagcg attaatgacc
     8161 gctttggtca tcaggcgggc gaccgtgttc tttctcatgc tgccggatta attagcagtt
     8221 ccttgcgtgc gcaggacgtt gccgggcggg tcggtggtga ggagttttgt gtgattctgc
     8281 caggcgcgag tctgacggag gctgcggaag tcgcagaacg tattcgcctg aagttaaatg
     8341 aaaaagagat gttgattgct aagagtacga cgatacgcat cagtgcctcg ttgggggtaa
     8401 gtagcagcga ggaaaccggt gattatgatt ttgaacaact ccagtcactg gctgaccgtc
     8461 ggctttatct cgctaaacag gccgggcgta atcgggtatg cgcgagcgat aacgcttaac
     8521 gagcggagaa aaaatggtcc agcccttcac gccagccttc cggtccttca cgctgcgttc
     8581 gccagacgcg ggtcggatcc tcatcatgca gatgcacccc ttcacggttt agccctttca
     8641 caatcaccgc gtaatccatt acctccagta agggcgcatc gtttggccca tcgcccaggc
     8701 caagtgtggt tgggcgtttg cctgataatt gttgataggt cgcgataatc cagttggcgg
     8761 cctgatcttt tccggcagag gcatccagga cgtgccagaa gcgcgcaccc tgcataaact
     8821 gtaagcccag ttcgttcaga cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc
     8881 agattagcgt taccgacgcc tcatgcagct gcgtcagcgc cgcctggcta cggcttaatc
     8941 ccgtccattc ggcgatggtt gcatcgtcga catcatcgaa agtcgtgaat ttaaaatgtt
     9001 ctttctcgcg tagcgtattt aaaacctggc tgatttcgcc atggctaata cctgagatga
     9061 tgcgtggaaa accgtctata tcctgccatt gttcagcaag ctggatcact gcgccatttt
     9121 cggcaatcag tggtaaccct tgtagcccca acgttttttg caagtacagc atttccgctg
     9181 aagttttact gctacagaga atgacgggaa cattcgcttc gcgtaaacgg ctgagccaga
     9241 gggcagtcgg ttgccagtca taactatgac tgtccagcag ggtgccatca agatcgctaa
     9301 aaaccagtag tggttgttga attgaaaaca tggtcgtgca gttctcccct taccaggatg
     9361 attctcaaaa cagcacaaat aatcaaagat gattttttcg ggagcgatcc cgttgacgcc
     9421 agagccacca aggggtgacg cacgtcacat ttctattcat aagtagcgtt aattattcat
     9481 atcgcgaata ttttcttgtc agcgaaaaaa attgcggata aggtgatgaa cacatcagat
     9541 ttcctggtgt aacgaatttt ttaagtgctt cttgcttaag caagtttcat cccgaccccc
     9601 tcagggtcgg gattttttta ttgtgcattc aacgattcac ttcagagacg ctgaagtcat
     9661 gaatatccag ttcgaaggca tccgccaatt cacgccatgt atgatattca cgtccatcga
     9721 cgctaacgtg ttcagaatcg ttctggctgc gatgaacttc gctttcgcta atttcattga
     9781 tcgccgccag cagggcatcg acatcgacgc tgacctcacg ttttgcggta tcgctgtact
     9841 cttttgcggt tttcatcatc tttcctcgca accgttactc tgttgttgcc gccagtcgtt
     9901 aagtccgcct ggcatctctt aagtatagac cgtcgagaaa atcagcactc gcgcgcgcct
     9961 ggagctgcca gaaaaacgaa attgttctac actggcacaa agccacagga ggaaaacgat
    10021 gaaggtgaat gatcgggtaa cagtcaaaac ggatggcggt ccgcgtcgtc ctggcgtggt
    10081 actggcagtt gaggagttta gtgaaggcac aatgtacctg gtttcgctgg aagactaccc
    10141 gctcggcatc tggttcttta atgaagcagg gcatcaggac ggtatctttg tggagaaagc
    10201 agagtaatct tattcagcct gactggtggg aaaccaccag tcagaatgtg ttagcgcatg
    10261 ttgacaaaaa taccattagt cacattatcc gtcagtcgga cgacatggta gataacctgt
    10321 ttattatgtg ttttgatctt acgtttaata ttacctttat gcgatgaaac ggtcttggct
    10381 ttgatattca tttggtcaga gatttgaatg gttccctgac ctgccatcca cattcgcaac
    10441 atactcgatt cggttcggct caatgataac gtcggcatat ttaaaaaaga ggttatcgtt
    10501 gtctcttttt tcagaatatc gccaaggata tcgtcgagag attccggttt aatcgattta
    10561 gaacttatca ataaattttt tctgaccaat agatattcat caaaatgaac attggcaatt
    10621 gccataaaaa cgataaataa cgtattggga tgttgattaa tgatgtgctt gatgtgctga
    10681 ctgttagaag catcatggat gaaacagtcc tcattaataa acaccactga agggcgctgt
    10741 gaatcacaag ctatggcaag gtcatcaacg gtttcaatgt cgttgatttc tcttttttta
    10801 acccctctac tcaacagata cccggttaaa cctagtcggg tgtaactaca taaatccata
    10861 ataatcgttg acatggcata ccctcactca atgcgtaacg ataattcccc ttacctgaat
    10921 atttcatcat gactaaacgg aacaacatgg gtcacctaat gcgccactct cgcgattttt
    10981 caggcggact tactatcccg taaagtgttg tataatttgc ctggaattgt cttaaagtaa
    11041 atgttgcgat atgtgagtga gcttaaaaca aatatttcgc cgcaggagta tcctggaaga
    11101 tgttcgtagg tattattttt cggataatcc ttaggataac atgataaacg ttacggaatt
    11161 atattaatgg caattcacta ataatatcag ccagcaaatt aaacatctca ctgaataaat
    11221 gttcgcaaaa aggtgcaatt aacggcatta atgccgccat taaagagata ccgacagtca
    11281 gagttaatgg aaatccaata acaaaaatgg ataattgcgg ggccatacga ttcagtaaac
    11341 ctaatgccag attcagggtc agcagcagag taatgagcgg taacgccagc atcagcccgt
    11401 taaggaaaat caaactcccc gctttggtga gtgccagaaa cgcattgctg ttcaacggtt
    11461 cgccaccaat tggcagggtg tgaaaggtat cgaccagcag tgaaatcaac cataaatgac
    11521 cgttaaatgt caggaacagc agtaacgcca gcatatccat gatacgcgct aaaacgggca
    11581 tattgagatg gctggccgga tcgacaaacg tcgcaaatga cagccccatt tgcagaccga
    11641 taatttcgcc agcggttcgc acagcggcaa aggcaaattg catggtaaaa ccaagcgcaa
    11701 tgccgatcag gatctgctgc acggccagcc acagagcaaa gaacgaaaaa acaggaacat
    11761 cgttggcagg taatgatggg gcaatgacga acgtgatcat cattgccagc cccagtttta
    11821 cccgtttcgg cacgctgcgt tcgctcagaa ttggtgcggt agaaatgagc gccagcacgc
    11881 gcagtaacgg ccagaagtac aggcttaacc aggaaagcca ttgatcgctt gtcacctgca
    11941 tcattgtacg gcctacccga tgatatacgg caggttagtg aacaaggtgc ggacgtaatc
    12001 cagcaacaga ttgagcatcc acggtccggc aataataatg gcaataaata cggcgatgat
    12061 ttttggaata aacgacagcg tcatttcgtt gatctgcgtg gcggcctgca aaatactgat
    12121 gataaggccc gtgaccagcg ccaccagcaa cagcggggca gccagtgcca gcgcgacttt
    12181 catcgcttca gtccccatca tcatgaccga ttcaggtgtc attttgcctc tctagctgta
    12241 aaagctctgc gccagcgaac cgaccagcaa ttgccagcca tccaccagta caaacagcat
    12301 cagtttaaag ggcagagcaa tggtggcggg ggggaccatc atcatcccca atgccatcaa
    12361 cacgctggca atcaccaggt cgataatcaa aaaaggaatg aaaatcgtga agcctatctg
    12421 aaatgcggtt ttcaattcgc tggtcacgta ggccgggagc aaaatgcgca tcggcacggc
    12481 ttcaggtccc tgcaacgggc cggtattcgc tagtctggca aacaacccta aatctgcctc
    12541 acgggtctga cgcagcataa actcacgtag cggctgcgcc cctttttcca gcgcctcctg
    12601 cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt tgtcgatcac
    12661 cgatgacata ataaaaaagg tcaaaaatag tgccagcccc agcaatacct ggttaggtgg
    12721 cgcggagggc gttcccagcg cgttacgcaa taaaccaaaa acaatgatga tgcgggtgaa
    12781 actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga acaccagcgt
    12841 ctgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg tgatacccgg
    12901 cagttgcgcg aaggcgaggg gcgtaaccag ccagagaagg acaggtgcga cagacaacaa
    12961 acgacgcatc aggatctccc actacgctta agcaaatttt tcatgaccga ctgaaaatcg
    13021 gtctgcggta tctcttctgt tggtgctgaa gggggaagtt tatgcagcag attgatttgc
    13081 cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac aacccgttca
    13141 cgcgcgccca gcgaagcact ggcgctaatt ttcagaccgt taacgccagt gcgtttcggg
    13201 gcaaatccca accgttttac cagccaggca gcagcgagga tcagggcaat aatggcgatg
    13261 agtgcgccgc tgacctgcag cagtggcgca gcagaaaccg gcgtggaaga ttgcacagta
    13321 gcgtggttat tcatcactaa cggctcaggc gacgcattcg ctcagacgga gtaatgatat
    13381 cggtgatccg cacgccatat ttatcggcaa cgaccaccac ttcgccctgg gcgattaaat
    13441 aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc acgacggacc
    13501 cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc tcgacggtca
    13561 gcttgaccgg aatatccatt atcaggtcga tatcctgcaa cgttccgctg acatcaccac
    13621 cgccaaattg ctggaacacc gcgtcggcag cgcttttgct gctggttgat ttttgttcgc
    13681 tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc ggattattca
    13741 tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa tcaaatgttc
    13801 tatccgtaac gcatactgac cgttgagagt gccgtactga ctggtcagca ccgggacgcc
    13861 gtcaacatgg gcgatgatgc catcgggttt ttctatcggc aggacgtcgc cgggtttcag
    13921 ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca gctccagctg
    13981 tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat tacgcgagtt
    14041 ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga atggcagaca
    14101 gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt taaccacaat
    14161 gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac gcacgtactc
    14221 aacttccagc ggattaatcg ccttccaggc gtcgctatag ccttcaagcg ccagctttaa
    14281 catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca ctttggtcgg
    14341 gaagcgtcca ttgccgccaa acaggttatc cacggcgata aacaccagac tcggtgaaaa
    14401 caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt tggtcggcac
    14461 cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga cggttatatc
    14521 cgggctacga cgcagcaggt tgaacagccc catacgaaaa tggcgggcaa agcgctcatt
    14581 aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat tcggatcgta
    14641 cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga cttcgctgtc
    14701 accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca tgtcgttatc
    14761 gcagaataaa agcggtatac atcacgtcgg tgacatcctg tttcggttgc ccggcaacaa
    14821 gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct tcttctgtcg
    14881 ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa acggctacgg acttccggca
    14941 aatactcact cagccgcgag cgggtagctt catctttgag tcgcagggtt ataccgatat
    15001 aaagcacgcg atccgcatcg cccaaattga ccgtaaaggt atccagcgca tagaagaccg
    15061 gtgagggcac gacgcgatgc tgcgctttgt cttcagcggc aacctgatgc gaatgccagt
    15121 agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc gggagccaaa
    15181 gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt cttcctgtgt
    15241 cgctactgct tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg ggaaaagacg
    15301 cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt tgcctgttac
    15361 gcgcccttgt aaagagacgg gaaccggaag cgtatcgtct tcttcccccg ccagaggttc
    15421 atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct gaccactaaa
    15481 gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctctcggcca gctgcgtacg
    15541 cagtaccggc agcgctgctt ccagggcggc gcgtacgtgc tgatgcggcg aaaccatctg
    15601 gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta aatcctgcgg
    15661 gtgcagacgc aactctgcac tttgttgtcc ctggcgggtg aacagcgaaa tatgctggct
    15721 taatgattgt tgccattcgt gagaacccag cggtgcactc aagacaggcg cggcgacggt
    15781 gagcagtggc tgtgtctggt gtggagtgat tagcgggctg gcggcagcgg tcaccggtga
    15841 aggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg ttaatggctg
    15901 agctggcgtg ccgggggcat catcaggctg tgctgttgtg agttgcgcag aagtcagttt
    15961 tgtgaagagc gttggtttct ctgccggtaa cacggttgac ggcgcatcag tcactttggg
    16021 cgtattgtca aaacccggca acatcgcaaa aagggcgctc aggcttgcgg tgaggtcttc
    16081 attcagatca tccgcttttt cgtcttttgt cgtatttttg tcagccaccg cagccaacgt
    16141 catcgtatga gcggtggtta acggtgtcga tgtggattgt tcgacgttga tgacaggcag
    16201 tgtttcatcc acagggatca gtaaatccgc ttgttgcgcg tcggaaacaa tatcgctgat
    16261 cagcagctcg ccttttgtcg tgggcttatc tgttgccacc agcacctggg gggccgcttt
    16321 gtcggtagtt gtctcgcttg ctaatgcttc gctcaacaac gcgagaaaat cttgtgcagc
    16381 atcgctggct ttgccgccag gcaatgtggt ggtgtcaacg tcggcggtaa tcaagggcgc
    16441 taagcgaatc attcaggttt cctcatggcg gcgcgctggg cgaactcatc catctttttc
    16501 tgatcgaggc ggttttctgc aagcagtgcc gccgtggatt gccgttcctg cagtgtctgc
    16561 caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc aactttctgc
    16621 gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt ctggataaac
    16681 tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct gttgaggtta
    16741 ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc ctgctgacac
    16801 ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc tgccagatct
    16861 ttcagggtcg ccagcgcacc atgttctgcc atctaccgtt atctcctggg ttatgacact
    16921 gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcacg ttcaaaaatg
    16981 ccttgttgta aatagccctc cagctgcggc cacagggcga tggctttatc gagcatcgga
    17041 tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg aaaactcgac
    17101 aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa cgccgtcatt
    17161 gcgcggctga tcgacgcttc aatatcgata gccggatagt gcccggcttc cgccagtcgg
    17221 cgagacagca caatgtgacc gtcgaggatc gcccgtgcgg agtctgcaat ggggtcctgc
    17281 tggtcatcgc cttcagtaag cacggtataa aacgcggtaa tcgagccgcc gccgctaatg
    17341 ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg tggataacct
    17401 ttggtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat cgcgtagcgg
    17461 gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc ttcggcaatg
    17521 cgcgtggcat aggcggcacc ttgcattcgc agaagcggag agacatccgc cggagcggca
    17581 atcaccacgg agcgtgcacg cccttcagca ccgaggatat tctcaataaa atcttttact
    17641 tcgcgcccac gttcaccaat caaacccacg acaatgacat cggcgcgggt gtaacgtgcc
    17701 atcatcccca gcagcacact tttaccaacg ccggacccgg caaacagccc catacgctgc
    17761 ccacgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag cacatgttca
    17821 atcggtgtac gttgcaacgg gttaaatggc ggggtaatca gcgcaccggt ttccgtcgta
    17881 tcgggggagg gcaggccatc gagcggctta ccgctgccgt ccagaacgcg acctaataac
    17941 gccggaccga gcggcaactg cttgccgctt tgcagtcctt cagccgaaat gtttttggca
    18001 taaacccgcg cgccgggcag gacaccttcc acttcctcca gcggcattaa aaacagccgt
    18061 tgaccgttaa agccaacgac ttcgctttct acttcgtgcg tttcgctgcc gttctggcgt
    18121 tcaatgacac aggttgcgcc gagcggcaat tgtaatccgg tggcttccag caccagcccg
    18181 gtagcgcggg ttaatcgccc atagcgacgt accgcaggca actgcgccat tttggcttca
    18241 aagttatcca gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc actcctggtg
    18301 ctgccagacg gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat
    18361 cggcggagac tttacagccg ccaggatgga gtgtgggatc gccccgcaag cgccagccat
    18421 gcaaacttaa ggttgcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc
    18481 gcagctgtgg tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga
    18541 tcagtgccga gttatccact gttggcgtct gaccgatcac ctgacgtgcc gcctccagcg
    18601 ccatctgcat caggcgcgaa gcaatcacac tatcaagcgc atcaagggta gtttgaaatt
    18661 cgctgaccag ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca
    18721 gaccttgctc cagcccctgg gccagtcctt cctgatagcc ctgctcatga ccttgctggc
    18781 gaccttcggc aatccccgcc tgataacctt gctcatgggc ctgcatttgc agttgcgcca
    18841 gttgctgctc aaggctaggt tcggcctctt caatgatggt ttcttccggc tcgaccatgg
    18901 gcacaaactc tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cacggcagat
    18961 tatcagacat aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg
    19021 cgcacaatca gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca
    19081 cggttggcga gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat
    19141 ttctcgcgca gtggctgctc ggcccctttc agcgcgatca acagcgattc ggaatccact
    19201 tcctgcaaca gacgctgaat gctgcgatcg tcgacatcca ccagattttc gaacaggaac
    19261 atctcgtcga taattttttg cgccagctcg ccgtcgaatt cgcgcacggc ggtaataacg
    19321 gcttcttcct gctgagtttt catcaggttg ataatttcgg ctgccgttct cacgccgccc
    19381 attttgctgc gcttgagatt ctgaccgtcg agcaagccat tcagtacttc ggtcagttcc
    19441 gccagcgcgg ctggctgcac gccgccaaag gtggcgatac gcaacatcac gtcgtggcgc
    19501 agacgttcat cgaacaacgc cagaatatcg gcggcctgcg cgcgcttcag atgcaccaga
    19561 atggtggcga taatttgcgg atgctcatcg cgaatcagat cggcggcact ctgcggctcc
    19621 ataaagttga gcgtttcaat accgctggcg gtatcgcgag tttcgagaat atcttccagc
    19681 aggctggcgg cacgttcctc acccagagct ttgaccaata ccgaacgcag ataatcgttg
    19741 gcgttgatat tcagtgcggc aaactgttca gcttcttgct caaactccgc cagcacatag
    19801 gttagctgct tgttggagat ctgcgtgacg ttcgccattg cagcgctcag ggtttgcact
    19861 tcgcgctggg agaggtgctt gaacacctct gccgcccggt cttcgccaat ggtcatcagc
    19921 aggatgacgc ttttatcggt gcctgtcagg ttactcatga tcgttattta tccactggcg
    19981 aatgaccagc gccaccacgc gcggatcgtt atcagacatt tcacggatac gctggctcat
    20041 gacttctgcc cccagacgtt ggttagcgcg tcgttgttgg agttgttcgt ctttgctcag
    20101 gcggacttcc accgcatctt ccacttcctc acgggcctgc gcctgttgct gtacagcttt
    20161 catcgcctcg gcgcgacgtg ttaactgcgg gcgtaccgct ttccgccaca gcagccacgc
    20221 caccagcagt accagcaacc agcgaccggc agccagcaac tgatcgataa acgcttgctg
    20281 ttgccagaac ggcagttctc cgccgctttc gtcactgcta ttgaacggcg agttaacgac
    20341 attgagcgag tcaccgcgtt tttcagaaaa gcccatcgcc tcgcgggtca gatcttcaat
    20401 ttgcttcatc tgttcgttgc tgagaggcaa tggtttaccg tctggcaagg ttttgtaatt
    20461 caccactacc gcgactgaca gacgttgcac atcgcccacg ttcattttgg tatgacgaat
    20521 ggtgcgatcg acttcgtagt tactggtttc attccgctgt gtgctacgcg gcccgctatt
    20581 gctggtggtg ctcgcctgct gctggcggtt attttgattt gccggaggcg tgctgattgg
    20641 cgcgttattc gcaggtgccg gttgattcga caacgccccc ggtacgccgc ccggataacc
    20701 ggaaccgctt tgctcgctct cattaagctg gcgtgaacga agcgccgcat gagattcatc
    20761 accgttaggg cgatactgtt cttccgtttg ttctttactg gcgaagtcca gttgcgccgt
    20821 gacctgggcg tgaatattac cgttaccaac aataggcgac aggattgctt caatacgccg
    20881 ctgaatacgg ccttcgacat cgctggcata tttcaactga gcgtcattaa gatcgcgccc
    20941 gctggtattg gactgggtta acagatgtcc gccctgatcc accagcgtga cgtttcccgg
    21001 cggcagacca gcaacggcgc tggaaaccag gtgcacaatg gcgctaattt gcccttcatc
    21061 gagtgcgcgg ccgggtaaca gatttaccgt caccgatgca gaaggggatt tttgttcacg
    21121 gacgaataaa gacggtttcg gcattgccag atgtacgcgc gcccctttta ccgggccgat
    21181 agtttcgatg gtacgagaaa gctcgccttc cagcgcccgc tgataattca cctgttcgct
    21241 gaactggctg ataccaaact tttcctgatc aagcagttcg aaaccgaccg cgccgccttt
    21301 gggcaaacct tgttgtgcca ggcgcagacg cagttcgtga actttatctg ccggaacttc
    21361 aatagcgccg ctggcttcgc tgaagcagta aggaatattc atttgcgtca gttggctgac
    21421 aattgcgcca ccatcctgat cggaaagatt gctgaataat gtgcggtagt cgggggcttt
    21481 cgcccacagg atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa caatcaatgg
    21541 aattttcgga ttcgcacgca ggcgattaag ccactcaaga gattttgtct gggctgcagt
    21601 cgcattcatc gcgcacctcg tggctgatta tggacggcgt tattggcaaa gtggaacaag
    21661 actcactccc gggtcagcaa actcgaaaaa ttgacgctgc cattatttgt gctttcgttt
    21721 ttttctatcc gccaataaac ccgttttttt tgttgctatt tagcgccttt gtcttattga
    21781 cttactggta agctttgcta ccagaaatta tccgggagat gaaaatgtca gcgatacagg
    21841 ggattgaagg ggttatcagc cagttacagg ctacggcgat gagtgcgcgt gcgcaggaat
    21901 cactgccaca accgaccatt agttttgccg ggcagctgca cgccgcgctc gatcgcataa
    21961 gcgatacaca aacagccgcc cgcacgcagg cagaaaaatt cactctcggt gaacccggcg
    22021 tggcgttaaa cgatgtgatg accgatatgc aaaaagcctc agtttctatg caaatgggga
    22081 ttcaggtgcg taataagctg gtggcggctt atcaggaagt gatgtccatg caggtgtagg
    22141 ttttgtaacc tgttgttaat tacatccgat ctcaccgcgt ggggcatgga tggggcaaac
    22201 tcactcaatt tctggtttaa aatggatacc tgatcctgaa agttttctgc catccacgat
    22261 ccgtaaacgc gataacatgt tttgtcttcg ctttgctcat aggaagagtg aactcttttg
    22321 ttagcctggg gttccttctg atcattattg ttcctgcctt tatctcttgc cacgccagag
    22381 ccccctggat acacatacac caggatgaaa acggtgagtt gtgttcaaac tgtagtacaa
    22441 ttctctccag tatgaacagg aaagaatatg ctatgaaccc ttatatttat cttggtggcg
    22501 caatacttgc agaggtcatt ggtacaacct taatgaagtt ttcagagggg tttacacggt
    22561 tatggccctc tgttggcaca attatttgct attgtgcatc attttggtta ttagctcaga
    22621 cactggctta tatacctaca gggattgctt atgctatctg gtcaggagtc ggtatcgttc
    22681 tgattagttt gctttcctgg ggattttttg gccaacgact ggacctgcca gccatcatag
    22741 gcatgatgtt gatttgtgcc ggtgtgttag tcattaactt gttgtcacga agcgcaccgc
    22801 attaaaaaat aatcatcctt gagataacag gaatctgatt tgtgcatatt atggctaatg
    22861 ttcggggcaa ctttataatt atgattgcat ttgcgcgatc attgcgcctt tttgcataat
    22921 gagatcaatg cacatcaaat aaaataacga taggtcacaa caaagaggtt tttatgaaaa
    22981 aacttatcgt ttcaagtgta ttggcattca taacattttc tgcgcaggcc gcagcatttc
    23041 aggtcactag taatgaaata aaaacaggag agcaacttac aacgtctcat gtattttctg
    23101 gagttgggtg tgaggggggt aatacatcgc cctcattaac ctggtctggt gctccagaag
    23161 gcaccaaaag ctttgccgta actgtgtatg atccagatgc gcctacaggc agcggatggt
    23221 ggcactggac tgttgctaat attccagcaa cggtaacata tttgcccgct gatgcaggaa
    23281 gacgtgatgg tacaaaactt ccgactggtg ctgtccaagg ccgaaatgat tttggctatg
    23341 cgggatttgg tggagcatgt cctcccaaag gagataaacc ccatcattac cagtttaaaa
    23401 tatgggctct aaaaactgat aagattcctg tggattctaa ttccagcgga gcgttagttg
    23461 gttatatgct taatgccaat aaaattgcaa ccgctgagat aatacccgtt tatgaggtaa
    23521 agtaggataa gagtatgctg gcaaaagata ggactaacct aaagattgaa gaaatacgga
    23581 tgcataaaca tcatgagatt cacagggtta agcctcttat gccagcttca tgtcgtatcc
    23641 gtcagggaaa aaaagttatc aattgggaga cgcatagtct aactgttgat aataatcaga
    23701 taatattatt tccctgtgga tatgaatctt atattgcgaa ttacccagaa gcaggtc
//
LOCUS       DAQSWQ010000061        23540 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.061, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000061 DAQSWQ010000000
VERSION     DAQSWQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.061"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..2064
                     /locus_tag="R8G18_002532"
     CDS             <1..2064
                     /locus_tag="R8G18_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HEE9796191.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="R8G18_002533"
     CDS             2080..3099
                     /locus_tag="R8G18_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HEE9796192.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="R8G18_002534"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="R8G18_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HEE9796193.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="R8G18_002535"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="R8G18_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HEE9796194.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="R8G18_002536"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="R8G18_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HEE9796195.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="R8G18_002537"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="R8G18_002537"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEE9796196.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="R8G18_002538"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="R8G18_002538"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEE9796197.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7646
                     /locus_tag="R8G18_002539"
     CDS             6828..7646
                     /locus_tag="R8G18_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEE9796198.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7698..8444
                     /locus_tag="R8G18_002540"
     CDS             7698..8444
                     /locus_tag="R8G18_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEE9796199.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8418..9383)
                     /locus_tag="R8G18_002541"
     CDS             complement(8418..9383)
                     /locus_tag="R8G18_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEE9796200.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9380..10384)
                     /locus_tag="R8G18_002542"
     CDS             complement(9380..10384)
                     /locus_tag="R8G18_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEE9796201.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10381..11658)
                     /locus_tag="R8G18_002543"
     CDS             complement(10381..11658)
                     /locus_tag="R8G18_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9796202.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11915..12967
                     /gene="fbaB"
                     /locus_tag="R8G18_002544"
     CDS             11915..12967
                     /gene="fbaB"
                     /locus_tag="R8G18_002544"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEE9796203.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13287..14141
                     /gene="gatY"
                     /locus_tag="R8G18_002545"
     CDS             13287..14141
                     /gene="gatY"
                     /locus_tag="R8G18_002545"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HEE9796204.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14170..15427
                     /gene="gatZ"
                     /locus_tag="R8G18_002546"
                     /pseudo
     CDS             14170..15427
                     /gene="gatZ"
                     /locus_tag="R8G18_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15437..15889
                     /gene="gatA"
                     /locus_tag="R8G18_002547"
     CDS             15437..15889
                     /gene="gatA"
                     /locus_tag="R8G18_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HEE9796205.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15920..16204
                     /gene="gatB"
                     /locus_tag="R8G18_002548"
     CDS             15920..16204
                     /gene="gatB"
                     /locus_tag="R8G18_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HEE9796206.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            16208..17563
                     /locus_tag="R8G18_002549"
     CDS             16208..17563
                     /locus_tag="R8G18_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEE9796207.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            17611..18651
                     /gene="gatD"
                     /locus_tag="R8G18_002550"
     CDS             17611..18651
                     /gene="gatD"
                     /locus_tag="R8G18_002550"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEE9796208.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18757..19530
                     /locus_tag="R8G18_002551"
     CDS             18757..19530
                     /locus_tag="R8G18_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEE9796209.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19612..20511)
                     /gene="yegS"
                     /locus_tag="R8G18_002552"
     CDS             complement(19612..20511)
                     /gene="yegS"
                     /locus_tag="R8G18_002552"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HEE9796210.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            20917..21234
                     /locus_tag="R8G18_002553"
     CDS             20917..21234
                     /locus_tag="R8G18_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796211.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21564..22925)
                     /gene="yegQ"
                     /locus_tag="R8G18_002554"
     CDS             complement(21564..22925)
                     /gene="yegQ"
                     /locus_tag="R8G18_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEE9796212.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(23072..23404)
                     /locus_tag="R8G18_002555"
     CDS             complement(23072..23404)
                     /locus_tag="R8G18_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEE9796213.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         6068 a         5514 c         6029 g         5929 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 acaccattca cggaatagat gtttcgcgct ggcaggagcg gatcgactgg cagcgtgtgg
     7081 caaaaatgcg cgataacggt attcgcttac aatttgcctt tattaaggcg acggaaggcg
     7141 aaaagctggt ggacccctat ttttcgcgta actggcaact aagccgcgaa aatggcttgc
     7201 tgcgcggggc atatcattat ttctccccgt cggtagcagc accggttcag gtgcgattat
     7261 ttctgcaaac ggtagatttc tcacaaggcg atttccctgc ggtactggac gtagaagagc
     7321 gaggaaaatt atcggcaaaa gaattacgca agagggtaag tcagtggcta aaaatggtcg
     7381 aaaaaagtac ggggaaaaag ccgattattt actcaggagc cgttttttat cacacgaatc
     7441 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctactatcaa cgtcgtccgg
     7501 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
     7561 tcaatggtcc ggtggatttt aatgtgttta atggcacggt ggaggagttg cagggattcg
     7621 ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca ccagactccc
     7681 tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa ctgaacgaac
     7741 gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg
     7801 ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt gatttaagtc
     7861 agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg gaagaagagg
     7921 gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc gaatactcgt
     7981 tgaaagaagc gcgaggtttt tctcagcagg tggtgttgcg cgggaaaaag cccgatacgc
     8041 tatgggttaa caagcgtgtc gtgaaatgtc ccgaagaagt cgcgcagcag ctggcggtcg
     8101 aagcaggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat
     8161 cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc atcgggattt
     8221 cactttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt tcccgcgtta
     8281 gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc aaaataccgg
     8341 tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag tacagcatta
     8401 gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc
     8461 gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc cagtaatacg
     8521 gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc atgactgtcg
     8581 cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg
     8641 ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc ggcaaatttc
     8701 tcctgccatt gctcgccaag tgttgttatc tctgcggata aagcaaaacg ttcggcggca
     8761 atctcagcct cttgacgatt gagcgacact aaaggtcgac aggccatgat ccgcgccagt
     8821 aatgcatccg ggatatcgcc aatacgtggg ccaaaatcga taaacggcgt cacgtcttgc
     8881 agctcttcca gccattccac taacaattcg ccgcagggcg aggccagttg ataaccggaa
     8941 aaatagagca ggctgccagg cgcaacggtt aatcgcgcca gccactggcg attccactga
     9001 ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac cagcgccaga
     9061 caccagccgt tatcaccttc ggcgttatcg atcaggctga ttaagccctc ttttgccatc
     9121 cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg
     9181 atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac gttaacgctc
     9241 tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc ggcgatcacg
     9301 tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc aggcagcagc
     9361 gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc gccagcgctg
     9421 tggcatagcg gttgaaatca agctgattta ccgcatccag ttccgccttt aacgcaggat
     9481 cgatagcgtt aacgccatgc aacgcgccgc aaattgccgt cgccatagca ccgatggtgt
     9541 ctgtgtcgcc gccaaggtta gcgcacagga cggcgcagcg attcgggtcg gtttgtgcta
     9601 gttcaaccag cgcaatggcg cacggaacgg actcaatagt gctggtacct gcgccaacga
     9661 cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attgcgcaca attttcagcg
     9721 caatctccag ccgcgccgcc agtgaggcgc tgaacgtagt gatgcgtttt tgctgtgcat
     9781 gtcgcgcaat tgaaggcagg gaatcaacaa tcgctgacca gctttctccg tcaatggcac
     9841 gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg
     9901 ggctggacgc cagcgctaca tcatcaataa aggaatcaac atcacgcgcc ggaagcaaac
     9961 aacctaatgg cgagacgcgc atcgctgcgc cgttggtcac gccgttgttt tccagttcag
    10021 caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt
    10081 ttttgttaaa ggcgtcgaaa cgcagcgccc agtcgagaat attacgccca atcagatccg
    10141 gatcgatctt gccttcacgt tccagtaacg catccgccag acacagcgcc atcgaggtat
    10201 cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggaa
    10261 gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg
    10321 gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt
    10381 tcatttaact tccccttgtg tcaacgcaat atcgcgatca tcgaccttga tagccgtaat
    10441 ttcgttgtcg gattcgcgga aaaaaatcat gaacagcacg gcgataatgg caatcatcac
    10501 cgcgccgaaa gtccacatcc cggaccagtt gaaagtcagt ccgtttaccg gttcctgata
    10561 agcgaacatc ttttccatca tcacaccgcc aagacgatag ccgagcaaac tgccgaagcc
    10621 ctggcagcag agcgtgatca gcccctgcgc agcggtacgc atatgcacgg gggctttttt
    10681 atcgacatag atgtaagcgg taacgtagta aaaatcgtaa cttacgccgt gaagcaaaat
    10741 accgaggaac agtaacgcgt aggtgaaata ttcatccgta ctaccgtaaa taaagaagcc
    10801 atagcggatc gcagcggtga ccagaccaag caataatacc tttttgatac caaagcgttt
    10861 agtgaaaaac ggcaatgcca gcataaagaa gatttcagag aactggccga gcgtcatcca
    10921 gccggtggcg tttttcatgc caacttcggt cagataaccg ttggcaaaga tgtaatagaa
    10981 cgccagtggc atcgcaaaca ggaatgaaca gaagaaaaag acgaggaagt ttttatcgcg
    11041 cagcaggatc agcgcatcca ggccgagcat gactttaata tccattttgc cggtgctttt
    11101 tggtggcgtg tcgggcagga aaaacgcaaa cacaccgagc agagcagaac ttccggcggt
    11161 aatcagcagc gggatgttag tcggtgagat atcggcatac cccagtattt gcggcaagaa
    11221 accacatgcc agaccggagg caatccagcc gatagtgccc atcacacgaa tgcgcgggaa
    11281 atcacgctcc acatccggca cgttggcaaa agcgatgctg ttagtcagcg caatggtcgg
    11341 catataggtt agagagtaag ccagcagtaa cggaaagaac ccggcaaaag tggtctgttg
    11401 cgcagcgaaa tacatcagca gcgcgcctgc gaacatcaat accgccagca ctttttgcgc
    11461 cgagaaaaag cggtcagtga tggagccaac cagaattggc gacaggatcg ccgcaatggc
    11521 ggtacaggca tacgaccagc caatttctcc ggcactaaaa ccgcttttac ttaaccacaa
    11581 ccacaatggc acaaaccacg cgccccagat aaaccattca acaaacatca tgaacgacag
    11641 ctttgctgtt gttttcatct tttaattcct tcatgacagg taatgtaagg tacgcgatga
    11701 caataccact taacaatacc ttttaaatac ctttgcggtg aaaacttgac cgcgctaaca
    11761 ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa acatccaaaa gatggaaaaa
    11821 ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg cgggccagga caatccatag
    11881 ccgatatcca atcggaattt acgggagcat agtaatgaca gatattgcgc agttgcttgg
    11941 caaagacgcc gacaaccttt tacagcaccg ttgtatgaca attccttctg accagcttta
    12001 tctccccgga catgactacg tagaccgcgt aatgattgac aataatcgcc cgccagcggt
    12061 gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag gatatctttc
    12121 tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg ctgctaaccc
    12181 gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct gtaactgtgt
    12241 ggcgtcaact tacggcgtgt tggcgtcggt atcgcggcgc tatgcgcatc gcattccatt
    12301 tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg atcaaacgct
    12361 gtatgccagc gtggagcagg ccttcaacat gggcgcggtg gcggttggta cgactatcta
    12421 ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc
    12481 gcacgagctg ggcatggtga ccgtgctgtg ggcctatttg cgaaactccg cctttaagaa
    12541 agatggcgtt gattaccatg tttccgccga cctgaccggt caggcaaacc atctggcggc
    12601 aaccatcggt gcagatatcg tcaaacaaaa aatggcggaa aataacggcg gctataaagc
    12661 aattaattac ggttacaccg acgatcgtgt ttatagcaaa ttgaccagcg aaaacccgat
    12721 tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt tgataaactc
    12781 cggcggtgct gcgggcggtg aaactgacct cagtgatgca gtgcgtactg cggttatcaa
    12841 caaacgcgct ggcggaatgg ggctgattct tggacgtaaa gcgttcaaga aatcgatggc
    12901 tgacggcgtg aaactgatta acgccgtgca ggacgtttat ctcgatagca aaattactat
    12961 tgcctgatac gcctcatacg aacagcacca ccccgtagga cggataaggc gtttacactg
    13021 catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta catagcactg
    13081 ccacgtatgt cggaaaaggc gtttacaccg catccggcat aaaaacacgc gcactttgct
    13141 acggcttccc tatcgggagg ccgttttttt gcctttcact cctcgaacag ttttcatatt
    13201 gtcgttttgt gatcgttatc tcgacattta aaaacaaatc atttcattat ttttttgaaa
    13261 tcgaaaacaa gcgacaggat atgaaaatgt acgtggtatc gacaaagcag atgctgaaca
    13321 acgcacagcg cggcggttat gcggttccgg cattcaatat tcacaatctc gaaacgatgc
    13381 aagtggtggt ggaaaccgct gccaacctgc atgcgccggt catcatcgcc ggaacgcctg
    13441 gcacattcac ttatgctggt acagaaaatc tgttggcgct ggtcagcgcg atggcgaagc
    13501 aatatcacca tccactggca attcatctcg accatcacac gaaatttgac gatatcgctc
    13561 agaaggttcg ttctggcgtg cgctcagtca tgattgacgc ctcgcatttg ccttttgcgc
    13621 aaaatatttc acgggtcaaa gaggtggtgg atttttgcca tcgctttgat gtcagcgtcg
    13681 aagcggagct ggggcaactt ggcggccagg aagatgatgt gcaagtcaat gaagccgatg
    13741 ttttttatac caaccccgct caggcgcgtg aatttgccga ggcaaccgga attgattccc
    13801 tggcggtcgc catcggcacg gctcatggga tgtatgccag cgcaccggcg cttgattttt
    13861 ctcgactgga gaacattcgc cagtgggtga acttaccgct ggtgctgcat ggcgcgtcag
    13921 ggttatcgac taaggatatt cagcaaacca tcaaactggg gatatgcaaa atcaacgttg
    13981 caacggagct gaaaaatgcc ttctcgcagg cgttaaaaaa ttacctgacc gagcaccctg
    14041 aagcgaccga tccccgggat tatttgcagt cggctaaatc cgcaatgcgc gatgtggtga
    14101 gcaaagtgat tgccgattgt ggctgcgagg gcagggcata acgcacctgc catttaacaa
    14161 ggaaaaaaca tgaaaacgtt aattgcccgg cataaagctg gtgaacatat cggcatatgt
    14221 tcagtctgtt ctgcccatcc gttggttatc gaagcggcgc tggcatttga tcgcaacagc
    14281 acgcgcaaag tgctgattga agcaacgtca aaccaggtca atcaatttgg cggttatacc
    14341 ggaatgacac cggcagactt tcgcgaattt gtttttacga ttgccgataa agtcggattt
    14401 gcacgcgaac gcattattct cggcggcgat catctggggc caaactgctg gcagcaagaa
    14461 aatgcggatg cggcgatgga aaaatccgtc gagctggtaa aagcatatgt tcgtgctggc
    14521 ttcagtaaaa ttcatcttga tgcgtcaatg tcctgcgcgg gggatcccat accgttagca
    14581 ccagaaacgg ttgcggaacg agctgctttg ctttgctgcg gaaagtgtgg cgacagattg
    14641 ccagcgtgag caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc
    14701 cagcgccatt cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac
    14761 gcatcaaaag gcctttattg cccgtgggct gacagaggcg ttaacacgcg tgattgccat
    14821 cgtggtgcag ccgggcgtgg aatttgatca cagcaatatt atccattatc agccgcagga
    14881 agcgcagccg ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac
    14941 cgattaccag acccggacgg cttattggga attagtccgc gatcactttg caatattgaa
    15001 agtcggtccc gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga
    15061 gcaggaactt atcgcccctg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat
    15121 gctcgacgaa ccgcaatact ggaaaaaata ttatcgtacg ggttttaacg attcattact
    15181 ggatattcgt tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa
    15241 aaatagcgtc gaaacgatga tggtgaatct tgaaggcgtg gacatcccac tgggcatgat
    15301 tagtcagtat cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatacc
    15361 gcatcagctg attatggata aaatttatga tgttttgcgc gcctatcgct acggctgtgc
    15421 ggaataagga cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg
    15481 tagcgaagtt ttaacccata tcggtaatga gatgctcgcc aaaggtgtgg ttcatgatac
    15541 ctggccacag gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca
    15601 gcacgccatt gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct
    15661 gttaaggcca acaaataaag ttcattttca gcaagcggat gatgataacg acgtggaggt
    15721 atcgttggtt attgcgttga ttgtggagaa tccgcagcag caattgaaac ttttacgctg
    15781 tttatttggc aagttacaac agcccgatat cgtcgagaca ctaatcactc ttcctgaaac
    15841 ccagttaaag gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta
    15901 acaataaaaa ggactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc
    15961 gacctctacg atggcggcgg aagaaattaa agagttgtgt cagaatcata gtattcctgt
    16021 tgaattaatc cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat
    16081 atgcaccact gccaaagtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc
    16141 ttttatttct ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg
    16201 gtgacctatg ttttcagaag tcatgcgtta tattctcgac ctcggcccta cggtgatgct
    16261 tccgattgtc atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa
    16321 agcgggtctg catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct
    16381 ggattccatt ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt
    16441 ggtcgatgtc ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt
    16501 ggcgattccg attgcgattc tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt
    16561 ggtaaatgtt gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcatct
    16621 ggcaaccggt tcatggatga tagggatggc aggtgtggta attcacgcgg cgtttgttta
    16681 taagctcggc gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc
    16741 tattccgcac ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat
    16801 cgagaaaatc ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt
    16861 tggtccattt ggcgagcctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct
    16921 cgcgggttac gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct
    16981 gctgatgcca cgggtgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc
    17041 ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc
    17101 attactgctg gggcatacgg cggtggtatc ggcaagcctg atttttatcc cgctcaccat
    17161 tttaattgct gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccaccat
    17221 cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat
    17281 ctcgggtgtc atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca
    17341 cacccaactg gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga
    17401 tcagggcggt tctcccatta cctggttact gattcaggtt ttctccacgc aaaatattcc
    17461 cggtttcatt attatcggcg caatttatct taccggtatt ttcatgacct ggcgtagagc
    17521 gcgtggtttt attaaacaag agaaaaccgt tctcgcagaa taatttttac ctgaggggga
    17581 ggttatcccc cttaacaatc aggagttttt atgaaatcaa tggtgaatga tactgatggt
    17641 atcgtgcgcg ttgcagaaag cgtcattcct gaaattaaac atcaggatga ggtgcgggta
    17701 aaaattgcca gctcgggctt atgtggttcc gatttaccca ggatatttaa aaatggtgca
    17761 cattattatc caataacgtt aggccatgaa tttagcggct atattgatgc ggtgggatcc
    17821 ggtgttgatg atttacaccc tggcgatgcg gttgcctgtg tgccgttatt accctgtttt
    17881 acttatccag agtgtttgaa agggttttat tcccagtgcg caaaatatga ttttattggc
    17941 tcgcggcgtg atggtggatt tgctgaatat attgtcgtta agcgaaaaaa tgtctttgct
    18001 ctacccacgg atatgcctat tgaggatggg gcttttattg agccgattac cgttggcctg
    18061 catgcttttc atttagcgca aggttgtgag aataaaaacg ttattattat tggtgccgga
    18121 accattggcc tgctggctat tcagtgcgct gtcgcgctgg gagcaaagag tgtgacggcg
    18181 atcgacatta gctcagaaaa actggcattg gcaaaatctt tcggtgcgat gcaaacattt
    18241 aacagtagcg aaatgagcgc gccgcaaatg cagagcgttt tacgcgaact gcgctttaat
    18301 cagcttatcc tcgagacggc tggcgtaccg caaactgtcg aactggcggt agagattgcc
    18361 ggtcctcatg cccaactggc gctggtgggc acgttgcatc aggatctgca tttaacatcg
    18421 gcaacgtttg gcaaaatatt gcgtaaagag ctgacggtta tcggcagttg gatgaactat
    18481 tccagccctt ggccggggca ggagtgggaa acggcgagcc ggttgctgac agaacgtaag
    18541 ttaagcctgg agccattaat cgctcaccgt ggaagctttg aaagcttcac ccaggcggtg
    18601 cgtgacatcg ctcgtaatgc catgccgggc aaagtgttgc tcattccatg aaaccgcggg
    18661 ccagcgtgat gctggcccgg tattgtgcaa aacagatcat tcaccaatgg tcccccttcg
    18721 tttacactag ccacaattga atatgggtaa atgacgatga attcattcga gcgaaggaat
    18781 aagatcatcc aattagtgaa tgaacaggga accgtgcttg ttcaggatct ggcgggagta
    18841 tttgctgcct cggaagcgac aatccgtgcc gatttgcgct ttctcgaaca aaaaggcgtg
    18901 gttacgcgct ttcatggcgg tgcggcgaaa ataatgtccg gtaatagtga aaccgagacc
    18961 caggaagtcg ggtttaaaga gcgatttcag ctcgccagcg cgccaaaaaa cagaatagcg
    19021 caggcggcag tcaaaatgat ccatgaaggg atgaccgtta ttctcgacag cggaagtacg
    19081 acaatgctta tcgccgaagg attaatgacc gctaaaaata tcacggtgat taccaacagt
    19141 ctcccggcag cgtttgccct ttccgaaaat aaagacatta ctctggtcgt ctgtggtggc
    19201 acggtccgcc ataaaacgcg ctcgatgcat ggttccattg ccgagcgttc attgcaggat
    19261 attaatgccg atttaatgtt tgtcggtgct gatggtattg atgcggttaa tggtattacg
    19321 acctttaatg aaggttattc gattagcggt gcgatggtca ccgccgctaa taaagtgatt
    19381 gccgttctcg attcatcgaa atttaaccgt cgcggtttta atcaggtact gccaatcgaa
    19441 aaaattgaca ttattattac cgatgatgct gtatcagagg tggataaact ggcattgcag
    19501 aagacacggg taaaattaat tacggtgtag tattgttctg tatgccggat aaggtattta
    19561 caccgcatcc ggcatttcgg attctccctg gttcaagaga gaatagtttg attaacgcaa
    19621 taatagacaa tctggtggta atcgacaacg caacgccgcc ggaagtattt caatatgaaa
    19681 gttttgtccg ctcaacggtt cgccatcaag attaaaagtg atttcgtgtg gggcttgtat
    19741 atcaaaccac gacgaagcgc cttcgataat attcgggtta tcttcgtcag attttaaggt
    19801 tgatacgaga gccggaagaa tttcatcgcc ggtaaaaatg cgcagttgca gcaagccatc
    19861 gttaattaac gcgttcggac acaattgctg accgccaccg gcctgacgcc cgttaccaat
    19921 accaatgacc agggcgtcac cttgccagtg aaagttttca ccgcggattt cacaacggtc
    19981 cggttgcaga gtatccatgc gcattaagcc atgaatgatg taagagacgc cacccagcgc
    20041 ggcttttaat ttttccggcg tttctgtggt aatacgcgtc ccaaatccgc ctgtcgccat
    20101 attaataaaa caggtttgtt tgttgacctg cgccatatct atcgcaatgg cgttaccggc
    20161 aattgccagt ttcagcgcct tatccagtgc ctcaggaatc cctacactgg tggcaaaatc
    20221 attggcggtt cctaatggca aaattcccag cgcgggtatg ccatccccct cacactgaat
    20281 caacgccgta gaaacttcat taatggtgcc atcgccacca ccggcaatca ccgttgcgac
    20341 gcccaacttc cgggcctcct ctacatatcg tgcggcatcg cctttctccc aggtgacccg
    20401 cacatggatc gtcattcctt cctcacgcaa cagcataatt gcttcgcgca agggtagatt
    20461 gtcagtactt ttgccattaa gaatcagtaa gctggcggga aattctgcca tgatcgtatg
    20521 tgcctttatg attggtctgt agatagtgta gagcagaaaa caaaaggtgg agtcagaaca
    20581 ggatgaaagt cggaggataa aaagtcagtt caagtatgga ggattcagtg gttaccacca
    20641 ggagcttgca atggggacag cgaatactaa cggctgccac cgataaattt caaaaaagag
    20701 catataccta atattcaact aaacagtggc atcttcaata taatatatta aagcccccat
    20761 ggagttaccc tgaagggcct caatgtccgt aattcctact tatgtaggaa atgttgtaca
    20821 gaacatttat tataatccta ttcaattata ataatcatgc cattattata tttaaacact
    20881 agagagtgtc gttggtattt aatgggggaa ggtgagatga aaaagatagc tgctatatca
    20941 ttaattagta tttttcttat atctggttgt gctgtgcata atgatgaaac aagtatcggt
    21001 aaatttggtc ttgcatataa aagtaatatt cagcgtaaac tcgataacca atactacacc
    21061 gaagccgaag cttctttagc taggggcaga atatctggtg cagaaaatat agtaaaaaat
    21121 gatgcagccc atttctgtgt tactcagggc aaaaaaatgc agatagttga cctgaagaca
    21181 gaaggtgcag gattacatgg cgtcgctcgt ctgacattca aatgtggaga gtgagaatat
    21241 tttttggtaa gcatcaaaca tgcgcgttct ggttgtgctt agccggaacc tgtgcgagca
    21301 cgatgccgtt acgtgaaagg catcgtgcta tgaagggaga ttctatcgat gtggtcaatg
    21361 gaagacggtg accagggata gggcttatgc ataaaaaata agcccgtgta agggagatta
    21421 cacaggctaa ggaggtggtt cctggtacag ctagcatttt atgggttatg tttttcagcg
    21481 aaacggatga taaccttaat aaatgcagct gtatgtgatc ggtttctaag aattttccat
    21541 ccgggaaaaa taatcgaaat taatcactta ccgtgtgggt tacgcgtggt ttccccggag
    21601 aaattacgca tcagcagcgc gtaatttagc tcaagatcct gcgggaccgg gagccacaca
    21661 gtataaccat cgcctggtgc gaccggcatc gcttcacctt tggcgttttc catgtgctca
    21721 agggtaaagt taatgttgcc ttgcggcgtc atcagctcaa ggctgtcgcc aacggagaat
    21781 ttatttttca ccgctaccgc tgcgaggtcc cccttgcgct caccggtaaa ctcaccaaca
    21841 aactgctggc ggtcagaaac tgaataaccg tattcgtagt tctgataatc gtcgtgagta
    21901 tgacgacgca ggaaaccttc ggtatagcca cgatgcgcca gaccttccag agtttccagc
    21961 aggctggtat cgaacggttt gcccgcagcg gcatcatcga tagctttacg ataaacctgt
    22021 gcggtgcgtg cgcaatagta gaaagattta gtacggcctt cgattttcag cgaatgcacg
    22081 cccattttgg tcaggcgttc tacatgggcg atggcgcgca gatctttcga gttcatgatg
    22141 taagtgccgt gctcatcttc aaacgcggtc atatactcgc ccggacgctg ggcttcttcg
    22201 atcataaaca ctttgtcggt tggcgcgccg atacctagcg tcggctcaac attttgcacc
    22261 ggaatcggct cgtacttgtg tacgatgttg ccaacgtcat cttctttccc ttcctggacg
    22321 ttgtactccc agcggcaggc gttggtgcag gtaccctggt tcggatcgcg cttgttgata
    22381 tagccagaga gcaggcagcg accggagtag gccatgcaca gcgcaccgtg aacgaagatc
    22441 tcaatctcca tatccggcac ctgattgcgg atctcttcaa tctcttccag cgacagctcg
    22501 cgagagagga tcacgcgggt caggcccatt tgctgccaga atttcaccgt cgcccagttt
    22561 acggcgttag cctgcaccga gaggtggatc ggcatttcag ggaagtgctc acgcaccagc
    22621 ataatcagcc ctggatcgga cataatcagc gcatccggcc ccatttccac caccggtttc
    22681 aggtcacgga taaaggtttt cagcttggcg ttgtgcggtg caatgttgac cacgacataa
    22741 aactttttcc ccagcgcgtg ggcttcattg atgccgagct gaagattttc gtggttgaat
    22801 tcgttgttgc gcacacgcag ggagtaacgc ggctggcccg cataaacagc atctgcgcca
    22861 taagcgaaag cgtaacgcat atttttcagc gttcccgccg gggaaaggag ttccggttta
    22921 aacataattc tctcgttctg atgacaggtc agctccgctt cacctgatga agcggttagg
    22981 ggagtgcccc cactttaagg gcgggcattg tagcgctgtg gggggatctg gtaaagcgcc
    23041 ggacgccgac aacccacgcc cggcatcgta attacgtatt gtctttcacc gtctggcttg
    23101 tgccattggc tttaacggag gcaattccgg tttcacgaga ttgcgcggtg gcgtacatct
    23161 ggctggagcc gataatttga tgattggcgg ctttcagatt gaaatagaat ttgccgttac
    23221 ttgccgtttt tttctcatag cgttcttctt gcgggctgtt gctacgcacc gacgcgatgc
    23281 ccttttccgc agaggctttt gatgtataca gctcgctggt gaggatagtc tcaccattgc
    23341 ccgcttttag cacaaaccgg aactgattat cactgctctt actgagttca aaccaaccag
    23401 ccataatgac tccttgattg aaattgcgta tcgaaaacag catctaaagg tgctgtttac
    23461 cgattaaagt atgagcgatt ttctgatgcc tgccagggcg gagagataat gtcagttggt
    23521 aatgactcca acttattgat
//
LOCUS       DAQSWQ010000062        23237 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.062, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000062 DAQSWQ010000000
VERSION     DAQSWQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.062"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="R8G18_002556"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="R8G18_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HEE9796214.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="R8G18_002557"
     CDS             509..1138
                     /locus_tag="R8G18_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HEE9796215.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="R8G18_002558"
     CDS             1165..2826
                     /locus_tag="R8G18_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEE9796216.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="R8G18_002559"
     CDS             3065..3124
                     /locus_tag="R8G18_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HEE9796217.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="R8G18_002560"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="R8G18_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HEE9796218.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="R8G18_002561"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="R8G18_002561"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HEE9796219.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="R8G18_002562"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="R8G18_002562"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEE9796220.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="R8G18_002563"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="R8G18_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HEE9796221.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="R8G18_002564"
     CDS             9507..10127
                     /locus_tag="R8G18_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEE9796222.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGHTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="R8G18_002565"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="R8G18_002565"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HEE9796223.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="R8G18_002566"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="R8G18_002566"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEE9796224.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="R8G18_002567"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="R8G18_002567"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HEE9796225.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="R8G18_002568"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="R8G18_002568"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEE9796226.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="R8G18_002569"
                     /gene_synonym="dan"
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="R8G18_002569"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HEE9796227.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="R8G18_002570"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="R8G18_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HEE9796228.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="R8G18_002571"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="R8G18_002571"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HEE9796229.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="R8G18_002572"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="R8G18_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HEE9796230.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="R8G18_002573"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="R8G18_002573"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEE9796231.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="R8G18_002574"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="R8G18_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEE9796232.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="R8G18_002575"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="R8G18_002575"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEE9796233.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="R8G18_002576"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="R8G18_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEE9796234.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5655 a         6331 c         5906 g         5345 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcacccagtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgccagt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccataccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc cttgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAQSWQ010000063        22935 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.063, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000063 DAQSWQ010000000
VERSION     DAQSWQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.063"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="R8G18_002577"
     CDS             complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="R8G18_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HEE9796235.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
     gene            complement(138..884)
                     /gene="cas5e"
                     /locus_tag="R8G18_002578"
     CDS             complement(138..884)
                     /gene="cas5e"
                     /locus_tag="R8G18_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HEE9796236.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="R8G18_002579"
     CDS             complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="R8G18_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HEE9796237.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1962..2498)
                     /gene="casB"
                     /locus_tag="R8G18_002580"
     CDS             complement(1962..2498)
                     /gene="casB"
                     /locus_tag="R8G18_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HEE9796238.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2495..4057)
                     /gene="casA"
                     /locus_tag="R8G18_002581"
     CDS             complement(2495..4057)
                     /gene="casA"
                     /locus_tag="R8G18_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HEE9796239.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4155..6854)
                     /locus_tag="R8G18_002582"
     CDS             complement(4155..6854)
                     /locus_tag="R8G18_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEE9796240.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(7049..7201)
                     /locus_tag="R8G18_002583"
     CDS             complement(7049..7201)
                     /locus_tag="R8G18_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HEE9796241.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(7466..8200)
                     /gene="cysH"
                     /locus_tag="R8G18_002584"
     CDS             complement(7466..8200)
                     /gene="cysH"
                     /locus_tag="R8G18_002584"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEE9796242.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(8275..9987)
                     /gene="cysI"
                     /locus_tag="R8G18_002585"
     CDS             complement(8275..9987)
                     /gene="cysI"
                     /locus_tag="R8G18_002585"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEE9796243.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(9987..11786)
                     /gene="cysJ"
                     /locus_tag="R8G18_002586"
     CDS             complement(9987..11786)
                     /gene="cysJ"
                     /locus_tag="R8G18_002586"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HEE9796244.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            12105..12467
                     /gene="queD"
                     /locus_tag="R8G18_002587"
     CDS             12105..12467
                     /gene="queD"
                     /locus_tag="R8G18_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEE9796245.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            12545..13816
                     /locus_tag="R8G18_002588"
     CDS             12545..13816
                     /locus_tag="R8G18_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEE9796246.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            13807..14067
                     /locus_tag="R8G18_002589"
     CDS             13807..14067
                     /locus_tag="R8G18_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEE9796247.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            14084..14659
                     /locus_tag="R8G18_002590"
     CDS             14084..14659
                     /locus_tag="R8G18_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HEE9796248.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(14807..15667)
                     /locus_tag="R8G18_002591"
     CDS             complement(14807..15667)
                     /locus_tag="R8G18_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEE9796249.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(15664..16443)
                     /locus_tag="R8G18_002592"
     CDS             complement(15664..16443)
                     /locus_tag="R8G18_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEE9796250.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(16421..17371)
                     /locus_tag="R8G18_002593"
                     /pseudo
     CDS             complement(16421..17371)
                     /locus_tag="R8G18_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(17358..18791)
                     /locus_tag="R8G18_002594"
     CDS             complement(17358..18791)
                     /locus_tag="R8G18_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEE9796251.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(18861..19646)
                     /locus_tag="R8G18_002595"
     CDS             complement(18861..19646)
                     /locus_tag="R8G18_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEE9796252.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVNGGYLVR"
     gene            19964..21241
                     /locus_tag="R8G18_002596"
     CDS             19964..21241
                     /locus_tag="R8G18_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9796253.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            21268..22758
                     /locus_tag="R8G18_002597"
     CDS             21268..22758
                     /locus_tag="R8G18_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEE9796254.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTGVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNWGMRIRSRAGRMRE"
BASE COUNT         5471 a         6087 c         5662 g         5715 t
ORIGIN      
        1 gagttcttcc cgacgataaa gaaattgcct ttctttgccg ccagggaaaa gatcccacag
       61 ccactgatgc atcacatatt caccgcgatc caccagatgc agcaactgcg caggcgaaag
      121 ctggccggta tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc
      181 ccctgattga cggtgcgttc cccaaacagc cactgctgac gatttaaagg aacatcccgg
      241 cggcgtaata ttttgctcgc gaccaggccg tcgtgtttcc cttcccacca gcattcatcc
      301 tgaagtttcg ggagtgagac tttcagttta tggaaatgat cctgatactg ttggtatgca
      361 ttacataagg catcacacgc gttgccttcg agcagtaacg gcgcaagagg taatgccagc
      421 ggatgacttt ttcgccccag ataaagcgga aaaactggat ggcgtaaacc gtcctgcaac
      481 tgttcgaggc tgtaaggcgc atcggctgtt gttgccaccg ccaccatcca ccaggcatcg
      541 gtgtagtagt cgcgccggga gataatcgcg ctcaaaagct cagggtcgct caactcttca
      601 cgacggctga aataacgtgc tttacgcacc tcttttggca tctggaccgt gtgataatcc
      661 cgtgcccagc gcgggttacg gctggcgcaa accaccagtg aatagtggcg attaaacgcg
      721 tttaagcgtt cggtatcatc acgccgaatc cctaccccgg cagccagtag ccccagcaat
      781 gctgagcgcg aaggaagttc atgggtatga cgaacttcac ctggagcatc gacaccccag
      841 gacgccatcg gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc
      901 gcagataaag tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc
      961 gcattcggtc ttctgttcgt agaccgtatt catgttttcg cgaagcgttg taatacgctg
     1021 caccgccaca tctaactgac gtgtgccatt aatgggttca tagaaagccg ccgccagaga
     1081 gcgtggttga tcggtgcctt tttctgccag cgcccaggaa gcataggcac ggctggcaaa
     1141 gctattttgt ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt
     1201 ctgattagct aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca
     1261 gatataggtg tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc
     1321 atcttcagag gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt
     1381 ttcactgaca ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt
     1441 attcgccagc atacgaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa
     1501 cgcgagatct tcctcttttg gcgcgcgctt ttcttctgcc agttgatgag caagcgcttt
     1561 tacggcgtca aattctgccg ggctgatatg aactaattgt tcagtttcgg cgttagtgag
     1621 cggatctttt ggttttttgt catttttagc tttcccaaga taatcagcaa tttttgccgc
     1681 ccattcgatg gcttttttat cttcaatccc tttctcaatc aggatagttg ccgcctcacg
     1741 cgcaatacgc ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga
     1801 agtgcgccac gcacgtttca gactttgcga ggaaacgcgc agacgcgttg ctccacccag
     1861 caccacagtt tttggcgcac cggtgtcatc acggttaagg ttggcagcag ggtaagcggt
     1921 taacacatga agctgaataa atgttgtcat gagatagtcc tttattgatt ttgttcgtta
     1981 tcggcgtcgc cagcctggta atattccagc gcccagcgga tacggataaa ttcggttggc
     2041 cgctgctggc ggcggtgatg attgagcagg tcgtcgctct cctggcacca gcggaaaatg
     2101 ccatctgcca gggagtcaag attgacggaa ccgtttaata atctgacggc gcggcgtaac
     2161 tggcgcagta attcatccgg tgtttttact gctgacaggc gagtaaaacg cccctttgac
     2221 attaccgccg ccagttgcgc ggcaaaaggc tgccgttcat caatggcttt aacattggcg
     2281 ctaactgcgg ctatcagcgc cagtgcggtg atacgccact caggttcatc ctgccacttt
     2341 atttgtctgt tcttcagaaa caggcgaaat ccatccgtca gacaaacatc attaaccgtc
     2401 gtactacgcc gcagactggc acgttcgccg cgtttctcct gcaattcctc gtgccatttg
     2461 cgcagcgtgg ctttgtgctc ctcttttaca atactcattc agcagcctcc tgctttttct
     2521 ccctggcggc tttagcactt tgcttctccg ccgatgttgt aaaatatttc ttgcgcgcgg
     2581 tcatgatgcg ttccaaatca acgggctcat aaggattggt gaatacacgc tcgtcaaaat
     2641 cctgacgcgc gaataaccaa atttccttat tccatttgct gagtaattca tccgcatcct
     2701 gaccttcttc aatttggcgc actaacctca ggaagcgatg ctgagttttg ttccagaagt
     2761 cgatatccac aaaactaaaa tcaccccttg caccttttgg atcggagaac catgcttctt
     2821 tcaatgcact ccgtaacaga cttagaatcc gtgaagccgt ttgcgcagcc agccgcagtt
     2881 tcggtatctg gccttctttt ttattgagca gcagcgggaa atgatgttcg taccaacagc
     2941 gcgctttcat gttgtcgaaa tcatagccaa atccccacag gcccactttt gcctgcttga
     3001 gactgctggc attaaagagt ttcaccacca gcgcgggaag ttccgtattg ttttctgact
     3061 tacccgtttc gataaggcct aaccagtcgc gccagattaa accgcccggt tgtggtttga
     3121 ccgagtaaaa ctcgccgccc tctttaagta gtacacggta aggcgttaag ggatgctgcc
     3181 acatggcata gttagcgccg taatttttgg tcgtcatcag gctcagaagc gcgtcgctct
     3241 gctcaccgca aatatcgcag ttgccgactg tggtggtatt aaaatcaata cgaatacggc
     3301 gaggcattcc ccagtacgcc tggagtttat tgacctgctc atcggttacc accgcaccgg
     3361 ccagttcgct gatgcgcgtc gggccaagcc aggggaaaac cagatcgtca aattttttgg
     3421 gtagcggtaa atcggcttca tcctgcggca tgacattgat ccacagtttg cgccacaagg
     3481 gagtttgttg attaccctga tattcctgta attcaatcag agtggtcatc ggcccaccgc
     3541 cgcgtaaacc ggtgcggtag cctttgccac ctgacggcgc attcaactgt agggagaaca
     3601 gagctaacgc agagcaatgt gggcacaggt attccgttac accacgcttg ataaagtggt
     3661 ctttattaaa cttcgttgtt tgagcgccgg gaatctcagg cagtagcgaa gcgaccggaa
     3721 ctttatctcc ggtgagcgcc tcgaaatcct gtataaatga aggggaatct ggcccaaact
     3781 ggaaagcatg ttctaatgac agcaatgctt cccgtagctt ttcagcttcc agtccgtctt
     3841 cccagatatc atcccaacga cgatgatctt ttggcgcgaa gctggtttgt agtagcccca
     3901 gcaaaaactg ccataccgca ccctggagat ctgcccgcgg cgcagaaata tcgacaacat
     3961 tttcatccgc cagatcgact ggcgccagtt tgcctgtcgt tccgtcttta aaacgaacgg
     4021 gcaaccacgg ggttgtcaga agtgaaaacg agttcatacg catcctctcg taaaaaagac
     4081 catcccaggt cggttgtcca tctttccatc cttttcggtg tatcaactat tcagagagtc
     4141 ctgtcgcaga catctcaacc aaccttgcca atcaatcctt ccgtagccga atacccgcaa
     4201 ctttcgtcat cgttgactaa aataacgttt gccatctccg gatcttgtcg ctgttcaatg
     4261 caccacagcc tgaacgcctc tccctccagt aaagaaaact catcccgatg ttttttccac
     4321 cagcttcggc gcactctgac aacgctcatt tcccatgcgt gagcaccggt ggcataaggc
     4381 ttcaccacac cgtcaataca ggtagccagc cacagggaaa cagactcctc agccagacgt
     4441 gtcgacagtt tttctgggag gtaatcgttg atattggcgg catagccggg cctgaagttc
     4501 agcacaaact ttttagccat tgcgcgatca cagtaatatt tgcccacttg ctcctgctcg
     4561 ctgcgggcaa atccttccgg cattaccacg tcctcaccgt agacggattc aataagaagc
     4621 cgggctgcgt gtggcatctg aatagcacct tgctcacgca gcacacgctg cgtcagccag
     4681 attcgcccat gatcgggata gacaaatgca ctgttacgca tggcactgcc aaaccattcg
     4741 tcgccaggag agtcgtccca gacgggagcc agaatcagca attcaggggg ggaacgctcg
     4801 tcctttccgt cacgctttaa ctgaccatta atatcgcgga tatgccgctg taatcgtcct
     4861 gctcgctgaa tcagcaaatc aacaggggcc aggtcggaga tcatttcgtc caggtcacaa
     4921 tcgacactct gctctaatac ctgagtacaa ataaggactt ttccggcacg ctgtgagcta
     4981 tcttctttac caaagcgtgc cagtgtctcc gtttcaattc gctggcgatc gctaaaagca
     5041 aagcggctat gaaaaagtga aaggctggaa gcaggaatga cgccgcgggc aagcagctga
     5101 cgatgaacct taatagcgtc atcgacagaa ttccggatcc aggcgatgca tttcccctga
     5161 cttaccgccg attcgatacg cgcaatacac tcttgttcac tatgaagcca acccacgctg
     5221 acgctacgct caacgtcttt gcgcgtcgct acccggtgtg agttcacatc ggatttcgtg
     5281 acatgcgtca gccaggggta atcatccttt tccaggaacg gagcttcttg caggccctct
     5341 gtaccccgcg caaaggcagc gacgagtttg tcgcgctgct gttgagataa cgtggcagaa
     5401 agcaaaataa cgctgtttcc gccacgcgcc tgccgctcga tcaacccttc aagaatgcac
     5461 gacatgtaag cgtcacaggc gtggatctca tcagccagca ggattttgtt actcagcccc
     5521 agaagccgca gattattatg tttgaacggc atcaccgcca tcatcgcctg atccagcgtg
     5581 ccgacgccaa tttcagccag caacgccttc ttgttactgt cggcaaacca ggccgcacat
     5641 ccctgactga acgtttgttc atccggttct tctgacccga ctaaatcacc agaccagagt
     5701 gattcattaa agcggtccat taatgtgcgg gcactatgtg ccagcaccag gctggggcgg
     5761 gattctggcg aatagaaagc aagccaggtt ttgaccagcc gatcgtacat ggcattggcc
     5821 gttgccattg ttggcagacc aaaaaacagc ccctgagcct tccccgcagc tatcaacctg
     5881 tgcgccagga taagcgccgc ttctgtttta cctgcgccag tcacatcttc cagaataaat
     5941 aactgcggtc cttgctggct gatatccaga tccagcacct tttgctgtaa cggtgtcgga
     6001 ttctcaataa aaggaaacag cgtattaatt ccgttgaaag gcgcgacttt tgcttttaga
     6061 ggaaagacgg ttaaggcgtt ttgagcctga actaaagttt tctgccagta acctttaata
     6121 tccattgggt gtgcgacgcg tggaaaaaat cgcgttgacg aacccgtcca gtctgcgagc
     6181 acgacggttg cagagatata ccaggaaagg tgttttataa gttcaatgcc ctcatcatca
     6241 tcccagaatg cggggatctc tatgagcgga aacagtggtt tgatttcaag gagaaaatct
     6301 cgtgctgcag ctttgtcttc aggcagaaaa ttatccaatt catccatacg atcagggggc
     6361 cgaccatgat gccctgtcgt cacgggcatc cacatttcta tcactcgcct aagtttgcgc
     6421 ggagagagcg aagatgaagg aagtaactcc tgacattcac ttaaataatg attccacaac
     6481 cagtagccca gcgttgaatg agagattttt tcgtaatttt ttctggcacc ttcctgaatc
     6541 ttgagttcag ggggcaggta aagttgttga aaagagcggg caaattttcc aatatcgtgc
     6601 cagcacagca accaggcgaa aaattgagcc gcctgttcct tgtcagaaat ccctaattga
     6661 cgaaaataat cagccagccc gaagcaattt cttttaacca ttaaatagcc catcgcggct
     6721 acatccagcg aatgccagca aagaaggtga tagtcgtcgc ccccgtcttt ctcgccgcgt
     6781 cgggtttttc cccagaaatc aaaaaaagtc acaatatttt tatccttcag taaacttaaa
     6841 ggatatttac gcatatatta aaaattatct gtgatatata tcattcaata aaatatttac
     6901 attatgaata atcaaataat tttcatccgt aatatatatg aataataaac ataggcataa
     6961 tattcacata aataatataa attaaaaaag ggtgcaggag accaaaccat cacaattcag
     7021 tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct
     7081 taaactccat accacgttct ctgatactca actcacacaa cgagtcgcct accatcagcg
     7141 tgaatcccag tactgtaaaa cacagcacta tgattgccac aagggcatat tttgtcagca
     7201 tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgctggc
     7261 ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta
     7321 acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt
     7381 tcacgtttaa aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag
     7441 aggcgtttaa tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc
     7501 caaagaaacg tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat
     7561 caccgaccga taaatatcct tcatcccata atgggtgata tttcaggcca tgtttttgca
     7621 ggtactgata aatagttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac
     7681 gctgaattgc cagcaccggt aaattggcgc gactgccgga ttgttcacgg cgcaggccag
     7741 caaaccaggt ttgcgcgtta agttctttca gagcccggtt catcggttcg actttgttga
     7801 tgtcattgta cttttcaatg ccttcaacgc cctgctccca cagttttccg tagcgtgctt
     7861 cctgccaggc tgcgctttcg gtagcacggt acactttcag gttgagcttg agtttgtccg
     7921 ttaactcgtc aataaagcgg taggtttccg ggaacaagta acccgtatcg gtgaggatca
     7981 ccggaatatc cgggcgaatt tgattcacca gatgcaggct caccgccgcc tgaatgccaa
     8041 agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggct acgcggcctt
     8101 cagcgtccag tttttccagt tcggcgttag tttccgccag cgccagaata cgatctacct
     8161 tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct
     8221 tgcctgatgc gacgctcacg cgttcttatc aggcctacaa gaccgggctg atggttaatc
     8281 ccacaaatcg cgcgccggat cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc
     8341 gccgaagcct tcacccgctt cgcgctcttt cgcccagcgc cctatcagtt catcaagcga
     8401 cgccaggatt tccggctcgg tgatgttttc tttatacatc cgtgggatac gtgtcccaat
     8461 gcggttgccg ccaagatgca ggttgtagcg acccggcgct ttacccacca ggcccacttc
     8521 cgccagcatc gcgcgaccac aaccgttcgg gcagcctgtt acacgcatca cgatatgctc
     8581 atcgctgaca ccatgtttcg ccattaaatt atcgatgttg tcgataaaag acggcaggaa
     8641 acgctctgct tccgccatcg ccagcgggca agtcgggaat gacacgcagg ccatcgagtt
     8701 ttcacgctgc ggcgtgacgg cattcattaa cccgctctct ttggcgatct tctcgatctt
     8761 cgctttctcg ctttccggta caccggtgat gatcagattc tggttcgccg taatgcggaa
     8821 atcgcctttg tggatcttcg cgatctccag caggccggtt ttcagcggac gccccggata
     8881 atcaaggatg cgaccatttt cgataaacag cgtcaggtgc cagttatcat caatgccctt
     8941 aacccagcca atacgatcgc ctcgtccggt gaactcatat ggacggatcg gttcaaattt
     9001 gatccccgcg cgacgctcca cttccgcttt aaacgtctca acccccacgc gctccagcgt
     9061 gtatttggtt ttggcatttt tacgatcggt tcggttaccc cagtcacgct gagttgtcac
     9121 gacggcttcg gccaccgcca gcgtatgctc cagcggcaga tagccaaact cgctcgccgt
     9181 gcgggcgtag gttttcttgt tgccgtgttc gatagaaagc ccaccgccca ccagcaggtt
     9241 gaagcctacc agcttgccgt tttcggcgat tgccacgaag ttcatgtcgt tggcatgcag
     9301 atcgatatcg ttctgcggcg ggatcactac cgtggttttg aacttacgcg gcaggtaggt
     9361 ctgaccaagg atcggttctt catcagtagt ggcgactttt tcctggtcga gccagatctc
     9421 cgcatacgcg cgggtacgag gcaacagatg ctcagaaatc ttcttcgccc actcgtacgc
     9481 ttccgcgtgc agctgcgact cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc
     9541 gttagctgtc gccagcgcat caagaccgac cgagtgcagc atctggtgca ccggtttgac
     9601 gttctttttc agaatgccgt ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc
     9661 atagatggtg ttttcaccgg caaatttgtc gatcgcctgc cactgtttag tggtaatcac
     9721 cccacccggc agacgacagc gaagcagcat cgcgtggcgc ggctccagct tctgttcagc
     9781 acgttcggca cggatgtcgc ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag
     9841 gaagttgtcg cccttaaagc cgccggtcag accgtcgttt aaatcttccg caatggtgcc
     9901 gcgcaggtag ttgctttcaa gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac
     9961 taaaggccct ggatgttttt cgctcattag tagacatctc gctgataacg gcgctctacg
    10021 cgcagctcac ttaaaaattc atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc
    10081 acttccagaa gtgcctgctc aacgtctttc gccatgcgat tagcgtcgcc gcagacataa
    10141 atgtgggcac cgtcattgat ccagcgccac agctccgcgc cctgttcgcg cagtttgtct
    10201 tgtacataaa ctttttcttt ttggtcgcgc gaccaggcaa gatcgatacg cgtcagcacg
    10261 ccctctttga cgtagcgctg ccactccacc tggtagagga aatcttccgt aaagtgcggg
    10321 ttgccaaaga acagccagtt tttacccggc gcttcgtcgg cggcgcgttg ctgcataaag
    10381 gcacggaacg gcgcgatgcc ggtaccgggg ccaatcataa tcactggcgt ttgcgggtta
    10441 gcgggcaggc ggaaattgtc gttatgttcg ataaatacgc ggacttcgcc ctcttcttcc
    10501 acgcgatccg cgaggaagct ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa
    10561 cgcaccacac caacggtgac gtgtacttcg ttctcgactt ccgcctgcga ggaggcgatg
    10621 gaatacaggc gcggggtcag cgggcgcagc aggttaatca gcgcttcggc atccagttgc
    10681 gccggagaga aacgcaccat gtcgacaatc ggcgtggtcg cggcgtaatg ctgtaacttc
    10741 gctttatcgc ccaccagcgg cagcagcgtt tcgctgcggg taagcgtggc atagttctca
    10801 acaatgttgg cggtgttgac ggtcagttcg aagtgccact gtagcgcttc gttcagaggc
    10861 aacgttttgc cctcgacggt gacaggttca tcgcctttca gccacagcag ttcgacaagt
    10921 tctttcacca gtgccgggtc gttctgatac cagacaccca gcgcgtcacc cggctggtaa
    10981 cgcaggcctg aatcgcctaa gtcaatttcg atatgacgaa cgtctttttc agagttacgc
    11041 ccggtaattt tctggttaac cgaaaggctc gccaccagcg gcgcgtcttt gctgtacggg
    11101 ctggtgtgga tttcatttac cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc
    11161 gcacgcgatt taagcgcatc aaccacgcgg gcgcgccact cgctggcagc agcctggtat
    11221 tcaacatcgg catcgacacg gtcgagcagg cgttcaccac ccagttccgc cagcttgctg
    11281 tcgaaatctt tcccggactg gcagaaaaat tcataagagc tatcgccgag gctaaacacg
    11341 gcaaacgcgg tgttttccag ctttggcgct tttttggaga acaggaactt atgcagcgcg
    11401 acggcttctt ccggcggttc cccttcccct tgcgttgacg tcactacgat gagcagtttt
    11461 tcgctggcga tttgtttgaa tttatagtcg cccgcgttca ccagcttaac gttcagtttt
    11521 gctgctaata aatcatcgcg taatgcttca gcaacccggc gcgcattgcc ggtttgcgag
    11581 gcggagataa tagttatacc cggcatttct gcggctggcg ctggcgtcgc tgcaagcgca
    11641 gcaggctgct gattgagtac gccccagaaa tagccagaaa cccaggcaag ctgggtaggc
    11701 gttaaatcgg tcgtggccgc ctgaaggcgt gccagttgct ccgggttcaa cggaagcaac
    11761 gcggaaggtg ggacctgtgt cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct
    11821 gcgccctgtc agcgcccata aaacagaaga gaagataagg ttaacggggt gaacggtgtg
    11881 gattaaagac gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt
    11941 ctttaacaac aaaatagatt aaccaaccca atgaaaaaca aatgaattta gccaatcatt
    12001 aagataaatc agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt
    12061 tccggtactc tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat
    12121 ttaaagattt caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat
    12181 gtggtcgcct gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc
    12241 cgcatacggg ctggattatc gatttcgctg aactgaaagc ggcgtttaag ccaacttatg
    12301 agcgcctcga tcaccattat ctcaatgata ttccaggtct ggaaaaccca accagcgaag
    12361 ttttagcaaa atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga
    12421 tggtaaaaga aacctgcacc gcagggtgta tctatcgcgg cgaatgataa gagtgtgtcg
    12481 gcggtcaatt tcccttaagt aacgctatgt tagggtgttg tgttctggat atctggggca
    12541 tgacatggaa gacgactgcg acattattat tattggtgcc ggtattgcag gcaccgcttg
    12601 cgcgttacgc tgcgcgcgag cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc
    12661 cggcagcaaa aatctttccg gcgggcggtt atatacccat gcactcgcgg aactcctccc
    12721 gcaatttcat ctgaccgcgc ctcttgaacg acgcatcact cacgaaagcc tttccctgtt
    12781 aacgcccgat ggcgtaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt
    12841 attacgtgca cggttcgatc cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg
    12901 catccccgga gcgacggtgg atgcactgta tgaagaaaac ggcagagtct gtggcgttat
    12961 ttgtggtgac gatattctcc gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt
    13021 cctggctgaa cgtcacgggt tagtgactcg tcctgctggc gaagcgatgg cgttggggat
    13081 caaagaagtg ctgtcgctgg aaacatccgc tattgaagaa cgttttcatc tggagaataa
    13141 cgaaggcgca gcgttgctgt tcagcggcgg aatctgtgat gacttacccg gcggcgcatt
    13201 tctttatact aatcaacaaa cgctctcgtt agggattgtt tgcccgctct cttcccttac
    13261 gcaaagtcgt gttccggcaa gcgagctgct gactcgcttt aaagcgcatc cggcagtgcg
    13321 cccgcttatc aaaaacacgg aatcactgga gtatggtgcg catctggagc cagaaggtgg
    13381 cttgcacagt atgccggtgc aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt
    13441 gcgcagttgc gtcaataccg gaatttccgt gcgcggcatg gatatggcgc tgactggcgc
    13501 acaggcggcg gcacaaacgc taataagcgc ctgccagcac cgcgagccgc aaaatctgtt
    13561 tgcgctttat catcacaacg tagagcgcag cctgctatgg gatgttctac aacgttatca
    13621 gcatgttccg gcgcttttgc aacgccctgg ctggtatcgg gcgtggcctg cgttaatgca
    13681 ggatatttcc cgcgatttat gggatcaggg tgataaacct gttccaccgc tgcgccagtt
    13741 attctggcgt catttacgtc gtcatggcct gtggcatctg gcgggcgatg ttatcaggag
    13801 tctgcgatgt ctgtagccca taatctctgg cgcgttgctg atgcgccgca cattgttccg
    13861 gctgactccg ttgagcgtca gatggtacaa cggttgatta acgcctgtcc ggcaggtctt
    13921 ttttcgctca caccggaagg tgacttacgc gttgattatc acggctgcct ggagtgtggc
    13981 acctgccgtt tgctgtgcga cgaatccaca ctacaacagt ggcgctatcc gccttccgga
    14041 ttcggcatca cctaccgctt tggataaata ataaggataa tttatgcccc tcttacacct
    14101 actccgccag aatccggtga ttgctgccgt taaagataat gccagcctgc aactggctat
    14161 cgattctgaa tgccagttta tttccgtgtt gtacggcaat atctgcacca tcagtaatat
    14221 cgttaaaaag attaagaacg ccgggaaata tgcttttatt catgttgatt tgctggaagg
    14281 cgcgtcaaat aaagaagtcg ttattcagtt tttgaaactg gtgaccgagg cggacggcat
    14341 tatcagtacc aaagcctcaa tgttgaaagc ggcaagagcg gaaggttttt tctgtattca
    14401 tcgcctgttt attgttgatt caatttcgtt tcacaacatt gataagcaag ttgcgcaatc
    14461 gaatccggat tgtattgaga tcctgccagg ctgtatgccc aaagtgctgg gctgggtgac
    14521 agagaaaatc cgccaaccgc tgattgccgg tgggctggtg tgcgatgaag aagatgcgcg
    14581 taatgcgatt aacgcgggtg tcgtggcgct ttccaccacg aataccgggg tctggacgtt
    14641 agcgaaaaaa ttactttgac gggataatcg taaccaattg aatttggttt gatttgtagg
    14701 ccgcacgcca catccgacat tcagcgcctg atgcgacgct tgacgcgtct tatcaggcct
    14761 acaagtcccc aggccagaac cgtaggcagg atcatccggc aacacgttat tggcaatctg
    14821 cgtgaatgtt tgtcaccagt gcctccagca ccactttcca gtcatcaacc acgccaacat
    14881 ctgcctgtga aaataccgca gcgctggcgt catgattaat cgccaccaca aacttgctat
    14941 tgcgtacgcc cgccattaac gctgctgcgc cagaagcgcc aacaacaata cacacctcag
    15001 gtgccagcag atgtccggaa ataccaatca ctttttcagc atcaacgcca ccgttcatca
    15061 ctcgcgcccg actgtagccc acttcggctc ccagcttttc tgccagcata gcgatttcct
    15121 ggttatctgc ctctccgccc tgccccacca ccagcacccg tctggcctca gctaatgggt
    15181 cgcgagtaac atttttcaga tcctctgtgc taaccagcca gtccggaagc gcacccggga
    15241 caatgttcag ttgttgcatt ccagagggca acgtggcgtt tttcgctgct cccgcctgac
    15301 gcgccaggga aaggcatagc gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc
    15361 cccagtggga tttgcgcacg cttacggttg gaatatctaa cgaagtcacc tggcagatgc
    15421 ttgcaccatg taaacgccag gccagccggg tcgacagttc atcgccaaac gttcccggcg
    15481 gaaagagaac aacgtccgca ggcgttcgct gccactgttc caccaacgca tccagcacct
    15541 gttccgccac tacaggctga ggttcaatct gccagtgcgc cagggtacaa ccgctgaaat
    15601 cctgcgccgc cagccagctt gctattgcgg cattctcctg atttattgtc acaattgcga
    15661 tattcatggc tgcatccttt ggcgtaaata gtcctgccat agcttttgag ctttttctgc
    15721 taccgtttgc ccatcaatca gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag
    15781 acactgcatc gctggcattt cggcagcaac agttttacgg ataatttctg ctttccccgc
    15841 agccattcgc tggcgcattc ccggtactgg caaggccact tcaccgcact gacgcacggc
    15901 aatcaccgca ggcaagcgaa cccggcaaca acgcaaccca tgttcagtac gctgttcgag
    15961 ggtgataaac agcgcgtcga gagtgaaacg ctccacctgg gtgaagcagg gccagcccag
    16021 catttccgcc agcaaaaatg gcgtttgccc attctgccct tcgctgcttt ggcagccggt
    16081 aattatcaga tccagcggat tctgatgctg ccattcggca atatggcgag cgacaaattc
    16141 cggcgcaaag cgcagatctg ccgccgtctc cagcaataca gcttcctcaa acccaagagc
    16201 catgagatag cgcagccagt gcaatgcccg ttcatccccc atgctcaacg cggttaaaga
    16261 catcggcgta ccgttttttc tctgcgccaa cagcagcgcg gcagcggcct gttcatcagc
    16321 accgagtaaa cttcgcagta gcgaaatatc cggtccgctt ttaccctgag ccgccgcctg
    16381 ccactctttt tccgccagca ttccggcatc cggttcggct ttaaacgcta acagaatgtt
    16441 cattcgcacc tcctacattt cccgccgcca ccagcgggag ggctttagtt tctggtgccc
    16501 atagccaggt cacaacaaaa tcaaccaaca acactgtcgc caggagcaat aaggtgactt
    16561 gcattcccca ctgcgccagc acccacggca gcaggccagt acttaccgcc gcgccaagtc
    16621 gactcatggc ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga aaactttccg
    16681 caggcaaaat gcccaccaga ttactgactg ccgaaatggt ggtgctgaag agaacaaaaa
    16741 gcagcagcgt taatgaactg ccggaaggca aacaggccat cactaccagc gttgccgcca
    16801 gcagcaaaaa acttcccagc aaaaatttgc gatgtgccag caggtgcgtc agaactaatc
    16861 ccagcagcgc gcccacaatt aacaacgcat taagcatcag gctggcagtc agcgcatctt
    16921 ccagaccaat agtctgggcg atagttggca gccaggtata aatcacaaac catgggatta
    16981 cgaggcagac aaagaagacg ctgttaaacg ccgtgcgccg ccagtaacgc gaagagaaca
    17041 aggttttgat gtgtttatgg gtcgccgtta ccacttcatc gcccagtaaa acatggggac
    17101 caaaatagcg atgcacgata gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc
    17161 gtggtgattc tggcgttccc cagcgtaata acgtaatcaa caacgcgggc agagctgccg
    17221 atgccagtag ccagcgccag gcctccgggt tttcggaaat aaagtgatgt ccggcaatac
    17281 ttgccagcac atagcctacg gtccacacca cgctgaatgc gcccagcaaa ataccgcgat
    17341 ggcggcgcgg ggaaaattca gccagcaagg ttaatgccat taggatcgaa ctgctttttc
    17401 agcccttcca gcaacgccca cgcgctgccg tgttccagtt tgctccagtg aacgcgatgt
    17461 ttaccgatac cgtggtggtg caccatcgaa ccgccgaggc gaatggtttc ttcacagatg
    17521 atcttgttga gcggattgtg gtacttgtcg atttcctctt ccggcttaca gtcaacgacg
    17581 ttgtaatcgt agacgaagta catgttggtg ccgttctgat agctatgaga ggaatgaccg
    17641 cccagcatgg tgatgtcgtc ggcgtgcggg aactcagtac gaatacggtt aataacgctt
    17701 tcgtagattt cgtggatgca gctccagcag ccggacactt cagtggtaaa gcccatgttg
    17761 ccggttttga ggatctgcac acgttcggca gccactttat ccggtcccca gttcaggttg
    17821 ttaaaccagg tttcgatcag cttgctgtcc acgcgctggc attgcgggta gcgggcaacg
    17881 atttccgcaa tcccttcgcc cgtcgccttc gcaatgcgag ggttaccttc agccataaag
    17941 atcagcacgc attttccgtc tgcaaaatgg gtgaagtgtt gggtgccatc ttcagcgtca
    18001 tacaaacgag cgatcgacgg acgataccct tccaccatga cttcacgcag gatgttgaag
    18061 tcggttttca tgtcttccag gatatagccg tagaagaggt tgttttccgg ggtgaattta
    18121 aagattttca ctgttacttc agtgatatag caaagtgcac cttcgttacc gatgatgatg
    18181 tggcgaatgt ccgggccagc cgcgcggcgt ggcacgtttt taatgcgtgt gacggtgcca
    18241 tcagccaata ctgcttccag accaacgacc atatcttcga ttgcgccgta gagtgtggag
    18301 aactgcccga tactgcgggt tgctaccaag ccgcccatct gcgccagcgg ctttgactgc
    18361 ggagaatgcc ccgtggtgta acctttttca cgcaacgcat tttccagcac ctccagcgga
    18421 acgccacatt gcgccgtcgc ctgcatattc tcaatatcaa tattaatgat ttgattcatg
    18481 gcggagccgt caagcaccac cgagttttcc acaacggttt ccagcccacc ttcagtggcg
    18541 gaagcgccgg tacgcggcac accgttaatt ttgtgcgcat tcataaaatt cagcacacgg
    18601 gatacttgct ctgtggaacc gagttttacg accgctgccg gaatcggcaa agtataaatg
    18661 ccatgaatat ccggaaattt acgaaaacgg tcaatactgt tcttctttaa tactgtttca
    18721 tcggtaatta cgcgatctgc accaacaatt tcctttagtt ggtcgacaat cgctgcgcga
    18781 gataaagaca tagtaattcc ttcctgataa taaaaatgga ataatcatta ataagagaaa
    18841 acagaaacca ccgttatgga ttagcgcact aaataaccac cattgaccac taataaatgc
    18901 ccattgacat aatttgaagc cggacttgcg aggaatacag ctgcgcccat taaatcctga
    18961 gtatcgcccc aacggtttgc cggaatatga tcaagaacgc gctgattggt ttctggatta
    19021 ctgcgtgtcg ccagcgtaat atctgttgca taatagccag gagcaatacc atttacctga
    19081 atattatatt gacctagttc atcacaataa gctttggtga atccggcaag agcatgttta
    19141 gtggcagaat atgcaggtga ccattgtcca cctaagtaag agaacaatga acagatatta
    19201 ataattttgc cgcttttctg cgggatcata atttttgcag cttcatagct taactcgaat
    19261 gcggcggtca ggttcacatc aatcatcgga tcccagtcgg cacgaccgaa gtccagcacc
    19321 ttattcagct tacaaatacc ggcattgttt accagaatat caactgtacc gaaacgctca
    19381 cagcaagcag cgataatctt ctgcggcgcg ccttctgcgg tgatatccac ctgcatgaag
    19441 tccacctcaa caccctgttt ttcaatcatt tcctttgttt cgccgttatc tttgacgaaa
    19501 ctaggaataa agatatttgc gccagctttg gccaacgcca tggcaaatgc ctggcctaaa
    19561 ccgctattcc caccggtaac aattgcggtt ttacctttca gtgagaaaaa atccattgag
    19621 aacgcattga gagattcgat tgacatagtt agctccaatt tccgtaaggc aaaaaaaaga
    19681 aaggtaactt cccccgcgag agggaagtta cctttctcat tatctctggt aactggagat
    19741 aatttttagc ataacggcat aactgctaat gtgatcgtaa tcacagtgtg atattcgtat
    19801 tgtcaaatag ttttaaaaag cagaagcgtg attactctca cacaacacct ctgctttcca
    19861 tgttatttct tattgcaatt actagagacc tgagaaaagt aatccacctg aaagggcggc
    19921 ttacttttct cttttttttg tctattacaa atgggaatac gatatgcaac acaactcata
    19981 tcgccgttgg ataaccctcg cgataattag ttttagcggc ggcgttagtt ttgacctggc
    20041 ttatttacgt tatatttatc aaattcccat ggcgaaattt atgggattca gcaataccga
    20101 gataggttta ataatgagta cctttggtat tgcggctatt attctttatg cccccagcgg
    20161 cgttattgcc gataaatttt cacaccgcaa aatgattact tccgcgatga tcattaccgg
    20221 attactgggt ctgttaatgg caacgtatcc accgctgtgg gtaatgctct gtattcaggt
    20281 cgcctttgcg ataacgacga ttttaatgct gtggtcggtg tcgattaaag ccgcctcgtt
    20341 gctgggcgat catagtgagc aagggaaaat tatgggctgg atggaagggc tgcgcggcgt
    20401 cggtgtaatg tcgctggcgg tgtttaccat gtgggtcttt tctcgctttg caccggatga
    20461 cagcgccagc ctgaaaacag tcattatcat ctacagtgtg gtttacatct tgttggggat
    20521 tctgtgctgg ttttttgtta gcgataacaa caacctgcgc agtgccaata acgaagaaaa
    20581 acagtcattc cagcttagcg acatccttgc cgttttgcgt atcagcacca cctggtattg
    20641 cagcatggtg atttttggtg tcttcacaat ctacgccatt ctgagttact ccaccaacta
    20701 tctgaccgaa atgtacggca tgtcgctggt ggcagcgagc tacatgggga ttgtgatcaa
    20761 caaaatcttc cgcgcgctgt gcggcccact tggaggcatt atcaccacct acagtaaagt
    20821 gaaatcccct acccgcgtga ttcaaatcct ttccatcatc ggtttgctgg cgttgacagc
    20881 cctgctcgtc acgaactcta acccccaatc ggtcgcgatg gggattggcc tgattttact
    20941 gctgggattc acctgttacg cctcacgcgg gctgtactgg gcctgccccg gcgaagcgag
    21001 aacaccgtct tacattatgg gcaccacggt aggtatttgt tcggtgattg gattcctgcc
    21061 ggatgtcttc gtttacccga ttatcggcca ctggcaagac accctgcccg ctgcagaagc
    21121 ctaccgcaat atgtggctga tgggcatggc ggcgctcggc atggtgattg tctttacctt
    21181 tttgctgttc caaaaaattc gtactgctga tagcgccccc gcaatggcta gcagcaagta
    21241 agccaggaaa attacaggag aaaaatcatg tcgaaaaaat acatcatagg gattgatggc
    21301 ggaagtcaga gcacaaaagt ggtgatgtac gatctggaag gtaatgtggt ttgcgaaggt
    21361 aaaggcttat tacagccgat gcacacgcct gatgccgata ctgcagaaca tcctgacgac
    21421 gatttatggg catcattatg ttttgcaggt cacgatttga tgagccagtt tgccgggaat
    21481 aaagaagata ttgttggcat cggcctgggc tccatccgtt gctgccgtgc gttattgaaa
    21541 gccgacggca cgccagctgc gccgttgatt agctggcagg atgcacgcgt tacacgccct
    21601 tacgaacata cgaatcctga cgtggcgtat gtaacctctt tttcgggtta tctgacgcat
    21661 cgcttaaccg gcgaatttaa agataatatc gccaactatt ttggtcagtg gccggtggat
    21721 tataagacct gggcatggag cgaagatgcc gcagtcatgg agaagtttaa tatcccccgt
    21781 catatgctgt ttgatgtgca aatgccaggc actgttcttg ggcatatcac acctcaagcc
    21841 gcgctggcga cgcatttccc ggcaggactg ccggttgttt gtaccaccag tgataaaccg
    21901 gtagaagctc tgggagccgg attactggat gatgaaactg cggtaatttc tcttggcact
    21961 tacatcgcat tgatgatgaa cggcaaagcc ttgccgaaag atccggtggc gtactggccg
    22021 attatgtctt ctattccgca aacattgctg tatgaaggtt acggtattcg caaaggcatg
    22081 tggacggtga gctggctgcg cgacatgtta ggcgagtcgt taattcagga tgccagggcg
    22141 caggatcttt caccggaaga tttactcaac aagaaagcct ctagtgtgcc acctggctgt
    22201 aatgggctga tgacggtgct ggactggctg accaatccgt gggaaccgta caaacgcggg
    22261 attatgatcg gctttgattc cagcatggat tacgcatgga tatatcgttc gatattggaa
    22321 agcgtggcgc tgacgctgaa gaacaattac gacaatatgt gtaatgaaat gaatcacttt
    22381 gcgaaacatg taatcattac tggcggcggt tcgaacagcg atctgtttat gcagattttt
    22441 gccgacgtgt tcaaccttcc ggcacgccgt aacgccatta acggttgtgc aagcctgggg
    22501 gcagcgatta atacaggggt aggtctgggg ctatacccgg attacgcaac ggctgtcgat
    22561 aaaatggttc gcgtgaaaga tatctttata ccgattgaga gcaatgccaa acgctacgac
    22621 gcgatgaata aaggcatttt caaagaccta accaaacata ctgatgtgat cctgaaaaaa
    22681 tcgtatgaag tgatgcatgg ggaattgggg aattggggaa tgcggattcg atccagagct
    22741 ggtcgaatgc gtgaataagg taggccgggc ggaaaactgc ccggcctgaa catacctgaa
    22801 tggttatccc cgctggcgcg gggaacataa gttcgacaac ttaaaaaatg ataacaagtc
    22861 cggtttatcc ccgctgatgc agggaacata ataaaaactt taactagttc ggtttatccc
    22921 tgtttttaca aggaa
//
LOCUS       DAQSWQ010000064        22592 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.064, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000064 DAQSWQ010000000
VERSION     DAQSWQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.064"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="R8G18_002598"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="R8G18_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HEE9796255.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="R8G18_002599"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="R8G18_002599"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HEE9796256.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="R8G18_002600"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="R8G18_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEE9796257.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="R8G18_002601"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="R8G18_002601"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEE9796258.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="R8G18_002602"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="R8G18_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HEE9796259.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="R8G18_002603"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="R8G18_002603"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEE9796260.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8676)
                     /gene="eco"
                     /locus_tag="R8G18_002604"
     CDS             complement(8188..8676)
                     /gene="eco"
                     /locus_tag="R8G18_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HEE9796261.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(8811..8975)
                     /locus_tag="R8G18_002605"
     CDS             complement(8811..8975)
                     /locus_tag="R8G18_002605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796262.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="R8G18_002606"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="R8G18_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HEE9796263.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="R8G18_002607"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="R8G18_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HEE9796264.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="R8G18_002608"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="R8G18_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HEE9796265.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="R8G18_002609"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="R8G18_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HEE9796266.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="R8G18_002610"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="R8G18_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HEE9796267.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="R8G18_002611"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="R8G18_002611"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HEE9796268.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="R8G18_002612"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="R8G18_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HEE9796269.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14943..15560
                     /gene="ccmA"
                     /locus_tag="R8G18_002613"
     CDS             14943..15560
                     /gene="ccmA"
                     /locus_tag="R8G18_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEE9796270.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="R8G18_002614"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="R8G18_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEE9796271.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="R8G18_002615"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="R8G18_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HEE9796272.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="R8G18_002616"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="R8G18_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEE9796273.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="R8G18_002617"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="R8G18_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEE9796274.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="R8G18_002618"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="R8G18_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HEE9796275.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="R8G18_002619"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="R8G18_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HEE9796276.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="R8G18_002620"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="R8G18_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HEE9796277.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="R8G18_002621"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="R8G18_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HEE9796278.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..>22592
                     /locus_tag="R8G18_002622"
     CDS             22225..>22592
                     /locus_tag="R8G18_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEE9796279.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5055 a         5669 c         6428 g         5440 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ttggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtctg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat gg
//
LOCUS       DAQSWQ010000065        22237 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.065, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000065 DAQSWQ010000000
VERSION     DAQSWQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.065"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            73..1461
                     /locus_tag="R8G18_002623"
     CDS             73..1461
                     /locus_tag="R8G18_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEE9796280.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1504..2007
                     /gene="yeaK"
                     /locus_tag="R8G18_002624"
     CDS             1504..2007
                     /gene="yeaK"
                     /locus_tag="R8G18_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HEE9796281.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2008..2112)
                     /locus_tag="R8G18_002625"
     CDS             complement(2008..2112)
                     /locus_tag="R8G18_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796282.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2282..2728
                     /locus_tag="R8G18_002626"
     CDS             2282..2728
                     /locus_tag="R8G18_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEE9796283.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2685..3506)
                     /gene="nimR"
                     /locus_tag="R8G18_002627"
     CDS             complement(2685..3506)
                     /gene="nimR"
                     /locus_tag="R8G18_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HEE9796284.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3603..4784
                     /gene="nimT"
                     /locus_tag="R8G18_002628"
     CDS             3603..4784
                     /gene="nimT"
                     /locus_tag="R8G18_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HEE9796285.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4839..5186
                     /locus_tag="R8G18_002629"
     CDS             4839..5186
                     /locus_tag="R8G18_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEE9796286.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5208..5462)
                     /gene="yoaF"
                     /locus_tag="R8G18_002630"
     CDS             complement(5208..5462)
                     /gene="yoaF"
                     /locus_tag="R8G18_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HEE9796287.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5645..6670
                     /gene="dgcP"
                     /locus_tag="R8G18_002631"
     CDS             5645..6670
                     /gene="dgcP"
                     /locus_tag="R8G18_002631"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HEE9796288.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6703..6801
                     /locus_tag="R8G18_002632"
     CDS             6703..6801
                     /locus_tag="R8G18_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HEE9796289.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6804..6878
                     /gene="yoaJ"
                     /locus_tag="R8G18_002633"
     CDS             6804..6878
                     /gene="yoaJ"
                     /locus_tag="R8G18_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HEE9796290.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6937..7185)
                     /locus_tag="R8G18_002634"
     CDS             complement(6937..7185)
                     /locus_tag="R8G18_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEE9796291.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7333..7515)
                     /locus_tag="R8G18_002635"
     CDS             complement(7333..7515)
                     /locus_tag="R8G18_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HEE9796292.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7519..7878)
                     /gene="yeaR"
                     /locus_tag="R8G18_002636"
     CDS             complement(7519..7878)
                     /gene="yeaR"
                     /locus_tag="R8G18_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HEE9796293.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8051..8689)
                     /gene="leuE"
                     /locus_tag="R8G18_002637"
     CDS             complement(8051..8689)
                     /gene="leuE"
                     /locus_tag="R8G18_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HEE9796294.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8816..9739)
                     /gene="dmlR"
                     /locus_tag="R8G18_002638"
     CDS             complement(8816..9739)
                     /gene="dmlR"
                     /locus_tag="R8G18_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HEE9796295.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9842..10927
                     /gene="dmlA"
                     /locus_tag="R8G18_002639"
     CDS             9842..10927
                     /gene="dmlA"
                     /locus_tag="R8G18_002639"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HEE9796296.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11178..12788
                     /gene="yeaV"
                     /locus_tag="R8G18_002640"
     CDS             11178..12788
                     /gene="yeaV"
                     /locus_tag="R8G18_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HEE9796297.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12820..13944
                     /gene="yeaW"
                     /locus_tag="R8G18_002641"
     CDS             12820..13944
                     /gene="yeaW"
                     /locus_tag="R8G18_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HEE9796298.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14000..14965
                     /gene="yeaX"
                     /locus_tag="R8G18_002642"
     CDS             14000..14965
                     /gene="yeaX"
                     /locus_tag="R8G18_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HEE9796299.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15019..16134)
                     /gene="rnd"
                     /locus_tag="R8G18_002643"
     CDS             complement(15019..16134)
                     /gene="rnd"
                     /locus_tag="R8G18_002643"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEE9796300.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16216..17901)
                     /gene="fadD"
                     /locus_tag="R8G18_002644"
     CDS             complement(16216..17901)
                     /gene="fadD"
                     /locus_tag="R8G18_002644"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HEE9796301.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18106..18687)
                     /gene="yeaY"
                     /locus_tag="R8G18_002645"
     CDS             complement(18106..18687)
                     /gene="yeaY"
                     /locus_tag="R8G18_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HEE9796302.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18727..19422)
                     /gene="tsaB"
                     /locus_tag="R8G18_002646"
     CDS             complement(18727..19422)
                     /gene="tsaB"
                     /locus_tag="R8G18_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEE9796303.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19480..21390)
                     /locus_tag="R8G18_002647"
     CDS             complement(19480..21390)
                     /locus_tag="R8G18_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEE9796304.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21522..21866
                     /locus_tag="R8G18_002648"
     CDS             21522..21866
                     /locus_tag="R8G18_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEE9796305.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5448 a         5698 c         5401 g         5690 t
ORIGIN      
        1 tttatcgccg caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc
       61 agcgacagag caatgagtgc ctatctgcgc tatatcgtgc agaaagcaga ctcctccttt
      121 ctttatgata agtatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct
      181 gagcagtcgg aagtgtcgcc agaacagcgg cgcgccatct gcgaggcttt tgagtctgcc
      241 aataataccc atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacacta
      301 caaaccgcat ccactgactg cgacacaatt gtggaagctg cagcactatt acccgctttt
      361 gatcaggcag tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc
      421 gaagagaaat ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg
      481 gttaatgttg aatactttgc gatgaataac tggtccttcc tgcagtcagg aagtattggc
      541 atcgatcgca aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac
      601 caggatcagc gtactaaaca gaacgtgatg agtttgctga cgccggtata tgtcgcaggg
      661 cagctaaaag ggattgtgct gctggatatt aacaaaaaca atttgcggaa tatcttttac
      721 actcatgacc gccctctcct ctggcgtttt ctcaatgtca cgctaaccga taccgattcg
      781 gggcgcgaca ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc
      841 catgacttac cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg
      901 tcttcgtgga aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtc
      961 cgaatgcact tccgtttata ccaaaatgtg tcgcgagaaa atattagtga tgcgatgact
     1021 ggactgtata atcgcaaaat tttaacccct gaactggagc agcggttgca gaaactggtg
     1081 caatccggtt cttcggtgat gtttattgca attgacatgg acaagttaaa gcaaataaat
     1141 gacaccctcg gtcatcagga gggggattta gcgattacgt tattagctca ggcgattaaa
     1201 caatcgattc gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt
     1261 cttgtcgatt cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg
     1321 caacatatcg cgccgcagaa agaaatcggc ttctcttccg gtatttacgc gatgaaagaa
     1381 aacgatacgt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
     1441 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt
     1501 gaaatgactg aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca
     1561 caagaaggtg ctaacttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
     1621 tcagaaattc gtggcaccgc tttaggccag ggagctaagg cactggtatg taaagtcaaa
     1681 ggcaatggcg taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg
     1741 agccaacttg ccagccatat aggcggatta cgcgcttcgt tagccagccc tgctgaagtc
     1801 gatgaactca caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc
     1861 aaactggttg ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggg
     1921 atgctggata aatccgttat tttgaaaacc acggattatt tgcgcattgc gcaaccagaa
     1981 ctgctcaatt tccgccgcac tgcgtaacta gcctgtccgt tcaataagca gaacggacaa
     2041 aaccagcaca acagcaatgg caaaaaacga tgacgtgata atcagtgtct cgacgaacat
     2101 ttgatcgttc atagcatgcc cccgggagat agctgttttc acgttattgt tagcgtgcac
     2161 aaatggcagt ttgatgacaa ttcgcctgat tttttatgca aaaaacgtaa agattttcta
     2221 cttccttcct gcagcaaacg taaagtaagc aggcttatta ttttttggca aggaaaccac
     2281 gatgtttgat gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca
     2341 taacaccact gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag
     2401 cacctttttc ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat
     2461 tggcgttatg gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt
     2521 tctgaactgg aaatcgctgg tggcaattgc agtaggcgtg attgtctcct ggctgggtgg
     2581 gcgcggcgtg accttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac
     2641 tgttttaggc gtggcgctgt ttcgcggcgt accggtcggc ccgcttattg ccgcgggtct
     2701 ggtttcgctg attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc
     2761 cccaggcctt tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga
     2821 gcaactttct gtacagtatc gcctttcact aatccctgaa gtgccataat cagctgtaac
     2881 tgctgacgcc actggcgaaa actcaacccc gtctctttta ctatcagtcg cgcaagattg
     2941 cgttcactca tcgcaaaaaa gccagcccat tgccctaatg acccccactc gacgggccct
     3001 ttcgccatca tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc
     3061 tgttgttgtg gctgctgagg gagttcatca aacaggacct gaattaatct gcgggtcatc
     3121 ggctcagcac gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag
     3181 ggcgatattt tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcgataaac
     3241 agaaaacaga gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc
     3301 caaaccgcat attgcggcgg caccatccac aaagcatttt ccaccgtaca ggtaattgca
     3361 ccatgtaatg ctagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca
     3421 tccgttccgg cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag
     3481 ccattgagat tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag
     3541 cttgattcaa cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc
     3601 atatgacctg ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc
     3661 tgatgattgc cacaacatta cgcgtcacct ttatcggcgc agcaccgtta ctggatacga
     3721 ttcgttccgc ttactcgctg acgacagcgc aaaccggctt attgaccacc ctgccattat
     3781 tggcctttgc gctaatctca cctttggcca ccccggtagc gcgacgtttt ggtatggaac
     3841 gtagcctgtt tgccgcgtta cttttgatct gtgctggtat cgcaattcgc tctctccctt
     3901 cgccttactt attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg
     3961 tcttactgcc aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg
     4021 catattccct gacaatggga gctgcagcgg cactgggatc ggctatgata gtgccgctgg
     4081 ctttgaacgg ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg
     4141 ctcttttttt atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc
     4201 gcgccttaca tactcggggt atctggcgtt cgccgcttgc ctggcaggta acattgtttc
     4261 ttgggatcaa ctcactgatc tattacgtga ttattggctg gcttccggcg atcctcatca
     4321 gtcacggcta tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag
     4381 cagctcccgg tttgctgatc cctcttttct tacatcatgt gaaagatcag cgtggtattg
     4441 cagcgttcgt tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc
     4501 acgcgatcac ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt
     4561 tgacgttcat tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg
     4621 cacaatccgt cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg
     4681 atgctaacgg tgactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg
     4741 cgattttcgg actttgcgcc gggagagaca aagaaattcg ctaatatccg gtgctatagt
     4801 gacgtgacaa atcatgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg
     4861 tttatgatcc ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc
     4921 gcggtatcaa aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa
     4981 cggaactgcg caaagccttt cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat
     5041 atcttgcaga actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa
     5101 aacagccgct gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc
     5161 tggccgactg gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca
     5221 gccggatggt cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg
     5281 caattgttgc tgaccccttg cgctgtcgac actggaatga gcgtcccgcc cttctgctgg
     5341 caatagaccg acgccggatt tgccatacca atctgcggcg gtttaggtac ttctggctga
     5401 gaagaggttg aacaaccagc caggaccagc aagcaaggca aagcaaaact gataattttc
     5461 atttattgat ctcactcatt tatctacgct tagagtatcg cggtatcgtt ttgttttgca
     5521 gcactatttt tattacattc actcaaaaca tattacgtct tgtttcatct ttgttggtga
     5581 tgttttatca tgcctgcaaa gattaaataa tcagcattta cccgccgtat cctggagttg
     5641 tttcgtgtca gatcagatta tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact
     5701 ggaaagtctg gtccgacagc ttctggagat gctggaaatg gtcactgata tggaatcaac
     5761 ctacctgacc aaagtggatg tcgaagcgcg cctgcagcat attatgtttg cccgtaacag
     5821 ccagaaaatg cacatcccgg agaattttac cgtctcgtgg gattactcgt tatgcaaacg
     5881 cgccattgat gaaaactgct ttttcagcga tgaagtccct gaccgttggg gcgactgtat
     5941 tgcggcacgc aatcttggca tctccacatt tctgagcaca ccaattcact taccggatgg
     6001 atcattctat ggcacgcttt gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc
     6061 ggaacaggtt ttacagttat tcgccggact gattgcacaa tatattcaaa aagaggcact
     6121 ggttgaacag ttgcgcgaag ccaatgctgc gctgattgcg caatcgtata ccgactcgtt
     6181 aaccgggttg ccgaatccgc gggcgatttt tgaaaatctg acgacgctgt tttctctcgc
     6241 ccggcatctt aaccataaga taatgatcgc gtttatcgat ctggataact tcaaattaat
     6301 caatgatcgt tttggtcata atagtggcga tctgtttctc attcaggttg gcgagcgcct
     6361 taatacgctc cagcaaaatg gcgaagttat tggtcgtctc ggcggtgatg agtttttggt
     6421 tgtttcacta aacaacgaga atgcggatat ttcgtcgctg cgagaacgca ttcagcagca
     6481 aatacgtgga gaatatcact taggtgatgt tgatttgtat tatcccggtg ccagtcttgg
     6541 catagtagaa gtcgatcctg aaacaaccga tgcagacagt gccctgcatg ctgccgatat
     6601 tgcgatgtat caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc attcagcgcc
     6661 acattgctga ggcgtattca catccttttg attggtgata acatgcgaat cggtattatt
     6721 tttccggttg taatctttat tacagcggtc gtatttttag catggttttt tattggcggc
     6781 tatgctgccc cgggggcata aagatgaaaa aaacaacgat tattatgatg ggtgtggcga
     6841 ttattgtcgt actcggcact gagctgggat ggtggtaacg tcacctctaa aaaatagcaa
     6901 aggctgcctg tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct tcctgtagat
     6961 aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat tgaagccatc
     7021 gactttacca aagccaaaca gcgtgctgat ccatccgccg actacggcac cgactatccc
     7081 cagcaggata gtcataaaga atccaccacc atctttacct ggcatgatcc acttcgccag
     7141 aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt cctcacttat
     7201 ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc gggagggcaa
     7261 gatttgtgac gagcatcacg gaggattttt ttgcgatggc gcagaaattg cgccatcaac
     7321 gatcagtgat aattaccaac cacaaacatc atgttcgttt tccgtgtcat aagaacgcac
     7381 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
     7441 cgtctctgtt tcataatcga cagaaacgcc attactgtta ttggtgacgg tcacggtata
     7501 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
     7561 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
     7621 ttcaatgttg tgccactttt ctggagggaa cacctcaaac tgccccgctt cgataaggat
     7681 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
     7741 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
     7801 tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
     7861 ctgtgggatt tgcagcatcc ttttccctcc ttcggtgaat gcgctgaaaa cggtttattc
     7921 cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt ggttactttt
     7981 tagcaccata tcagagaaaa atcagggagg attacaagtg ggaagcccgc gacaacgcgg
     8041 gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg aacatcaaac
     8101 caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
     8161 aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
     8221 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
     8281 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
     8341 aaatggcccc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
     8401 cgtagagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
     8461 tgaataatat cggggtggtc ttaattaatg tcgccactcc agcccatgcc agaaacatca
     8521 atactgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
     8581 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
     8641 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
     8701 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
     8761 agaaaggaga aaatcaggtt ttaacccgat atcaacccga taattgaata attaatcagg
     8821 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctatcg
     8881 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
     8941 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
     9001 cccaccagga gcgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
     9061 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
     9121 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcggaaa
     9181 gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa ttccggcgac gcgcagagaa
     9241 tgcggtaatt agtcgccagt ttgcgggcga ttaaattagg ggcgatgtca tcgccaatgc
     9301 gaatatcgag atcaacacct tcattgacca aatcgaccaa ccggtcttcc acatcaaaac
     9361 gtaattccag ttgcggatac gccttagcca gcgctgataa cgccggagcc accacctgtc
     9421 gtccaaaacc aaagctgctg ataatacgca gcatcccctg cggcacctga cgcacgtcag
     9481 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
     9541 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
     9601 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
     9661 ccgccacggc ggcaaaacca gcccggcgag ccaccagcat aaagacgcgc aaatcgttca
     9721 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
     9781 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
     9841 aatgatgaaa acgatgcgta ttgctgcgat cccgggagat gggattggca aagaagtcct
     9901 tcctgaaggg attcgcgtat tacaggctgc cgctgagcgc tggggcttcg acttgagttt
     9961 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
    10021 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
    10081 ggataccgtt ccggaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
    10141 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
    10201 gggaaaacag cctggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
    10261 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
    10321 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
    10381 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
    10441 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagatcc gctgggacaa
    10501 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
    10561 ggtggcgtcc aatttgtttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
    10621 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccgt cgctcttcga
    10681 gcctgtccac ggttccgcgc cggatatcta cgggaaaaat attgctaacc ctatcgccac
    10741 aatttgggcc ggtgcgatga tgctcgattt tctcggcaat ggcgatgagc gtttccagca
    10801 agcgcataac ggtattctgg cagcgattga agaagtgatt gctcacgggc cgaaaacacc
    10861 ggatatgaaa ggcagtgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
    10921 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttt ttattcgttc
    10981 tttgcagtta ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
    11041 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattt gttaaacaca
    11101 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
    11161 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
    11221 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
    11281 ccgacgcggc caacacaata tacacttttg ttacgcgtac gttaggttcc gccgtacagg
    11341 tattggtttt gctggcaatg ggactggtga tttatttagc caccagtaaa tacggcaata
    11401 ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc ctggctgttt atgtttattt
    11461 gtgccggttt aggttcttct acgctttatt ggggggttgc tgaatgggcc tattattatc
    11521 aaacgcctgg attaaatatc gcaccacgtt cacaacaggc actcgaattt agcgtccctt
    11581 actccttctt ccactggggc atcagcacct gggcaactta tacgctggcc tcattaatca
    11641 tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag tctttccggc attattgccg
    11701 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgtttctga
    11761 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
    11821 gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
    11881 tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt ttgcaacgtc
    11941 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggtc
    12001 caaccgaatt tattaccaac agcatcatca atgccatcgg cctgaccacg caaaacttcc
    12061 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
    12121 ttttctactg gctgtggtgg atctcataca cccctggcgt agcaatgttt gtcacccgcg
    12181 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
    12241 gttgctggtt cttcttcggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
    12301 taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgta cagcaagttc
    12361 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggcgtg atgattattt
    12421 tccttgcctc gcatatggat gcagtggcct acaccatggc ggcgaccagt acgcgtaatc
    12481 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
    12541 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
    12601 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
    12661 ggctgaaaca ggattatgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
    12721 caccggttga agccctggaa aaaacaccag tgctccctgc gggaaccgta ttcaaaggcg
    12781 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
    12841 tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt
    12901 ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg
    12961 atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac gcgtgagatc
    13021 attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
    13081 gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt
    13141 acctgcccat atcacgcatg ggcattcaaa ctcgatggca atctggccca tgcacgtaac
    13201 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggaa
    13261 gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcag
    13321 ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
    13381 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
    13441 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgc
    13501 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
    13561 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
    13621 tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
    13681 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
    13741 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
    13801 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
    13861 atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt ccataatctg
    13921 ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc gctgggctga
    13981 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagtcaggt
    14041 ggaaccgctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg gcaaaccgct
    14101 gcctgcgttc accggaggaa gtcacatcat tgtgcagatg agcgatggtg ataaccagta
    14161 cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc agattgccgt
    14221 tcggctggag gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
    14281 cgatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
    14341 gcatctgttt atcgcgggcg gtattagtat cacccctttc ctgtcgcaca tggcagagtt
    14401 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
    14461 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gtccatttgc atcattcatc
    14521 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
    14581 ttatacctgt ggccccgagg cgctaaatga agcggtaaga agtgaagctg cgcgtctgga
    14641 catcgccgcc gatacgctgc actttgagca atttgctatc gaagacaaaa ccggcgatgc
    14701 ctttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
    14761 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
    14821 atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg atcagtattt
    14881 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
    14941 taaacgcctg gtgttggatt tgtagtttgc caaagccgga tgtggcgctg agcgcgccca
    15001 gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
    15061 catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt
    15121 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
    15181 cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaacg ctttacgata
    15241 acccggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
    15301 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
    15361 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgcgaccg accacaaatg
    15421 ctcttcacgc acgacaaagt tcaccgccag atcacgctca cgagccttgc gcagtcgcca
    15481 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aattgccagg cattggtgat
    15541 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcggca
    15601 ttcatccagc gccgcaggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
    15661 gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt cggtcagcgg
    15721 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
    15781 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
    15841 gtcaatcagg ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
    15901 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
    15961 gtcggtgatc ccgagaggat caattagcgc cagatgctca ccatcgaaaa gttgaatcaa
    16021 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatcctggg ctatcgccgg
    16081 aaagacacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
    16141 attcaaattg tattctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
    16201 cgactgactt aacgctcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
    16261 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
    16321 cggtactttg tagcccgtga gctgacggca gcaaaaggtc accagtgact cttcggtaag
    16381 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
    16441 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
    16501 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
    16561 gaacccttct tcatccatca ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat
    16621 ctcatcggta gcatcgggac gctgccagta acccagcatc acctgcggtc ctttgacaca
    16681 aagctcaccc ggttgacccg gtggtacttc attatcgtcg tcatccacca gtttggcttc
    16741 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
    16801 gctgaccagc ggcgcacact cggtaagacc atagccttcc agcagatact gtcctgtcag
    16861 tttcacccaa cgctctgcca ccacttgctg cactggcatc ccaccgcctg cggaaagatg
    16921 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
    16981 gttaacgcca gtgatagcgg taaacggata tttcgctaac tcttttacca accctggaat
    17041 atcgcgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
    17101 aatggtcagg gcaaaaatgt gatacagcgg cagtgccgtt actaccagtt ctttacctgg
    17161 atgcagcaac ggcccatagg tggcgttgac ctgttccagg ttcgccagca tattgcggtg
    17221 agtcagcatc gcgcctttcg ccacacctgt ggtgccgccg gtgtattgca ggaaggctaa
    17281 atcttccggc accagttcgg gtttgacgta ctgcatccgg tagccgttat gcagtgcgct
    17341 acgaaatgaa atggcatctg gcagatggta tttcggcacc aaacgcttga tgtatttaac
    17401 aacgaaattg actaccgtgc cttttgccgt agatagctga tcgcccatac gggtcagaat
    17461 tacgtgctga acggcggttt tatcaaccac tttttccagt gtgtgagcaa agttagacac
    17521 gataacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac gcggggtata
    17581 caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg
    17641 atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca gccccaaccc
    17701 ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcgga aggtcattac
    17761 ctcccccata ttcacaaacg caggctgatc ggcgtagcgg acgaccgact gctcaaacat
    17821 atctatcaga gattgataac ggtcagggtt gatctccgtc ggaacatccg cgggataacg
    17881 gttaagccaa accttcttca attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac
    17941 cccaagtgat aaacatgttg ttaacataac attaactcat cttaccagct tgataattag
    18001 acaacgaaaa ggttgcgaag cgcgtcacta tttattttta tagttgccgt atgaacgcag
    18061 aaacagcgga ccagccgctg tttctttttc tttaaaaaca acttgttact cggttacaat
    18121 tgtttcaact cttgcagggc caggattata ccaaccccat ccgccgtaac catagggcca
    18181 gccgcgtcca ccataaaacc atggatcaat tggctgcggc ggcataatca cctgttgagt
    18241 gatatgccag cgtttgtaac ctgttacctg cataaccata aatttatatg gtgcattatt
    18301 tactttgcca tccactgtgc cagtgattgg cccgacaacc gtcaccagtt gtccacgaaa
    18361 atcgaccgga tcaagaaaac cgttcacatc ggcataaatg cgcccgcgtg aaggttctcc
    18421 caaggttggt cttgctccgc tgtccagcgg tacggtagca atttccaggc gggttttccc
    18481 ttgctggttt tgtaccacaa ccactttgcc gccaaagcgc gcctcctgac caacatataa
    18541 atctggcgca ttcatcacgc gtaccagatc ttgctgtggt gttggactgc tgcctttaat
    18601 ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg ccagtatgcc
    18661 tttgataaca ttcttttgaa ccgccatgat gcgactcctt tttttctcag ggcatactct
    18721 taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta aataaaccgg
    18781 ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacacg caatcggcag
    18841 catatcttcc gccgctggca gtaaaacttc tccgtcgcgc aaaaccagcc cgctctcttt
    18901 acggagatcc ggccaggctt gccagcccgt ccctaccgtt acccattcgc cggaaagctg
    18961 ttgcattcgt tcatgaacga gttcgggttt gagtacggct tcggtttctt caccgcgcca
    19021 gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc gcgcgtcaat
    19081 ggctgccagc acgcgggttg cgccgttttt gcgccacgcc ccctgcgcca tcgtcattag
    19141 cgtagagaca ccaatcatcg gtaactccgc gccaagcgcc agcccttgcg caataccaat
    19201 accaatgcgc acgccagtaa agctaccggg gccgcgcccg taagccagag cgtttatgtc
    19261 agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga ttcgttgagt
    19321 atgttcacga gggcaaagct caaaatgagc gttgacatta ccgtcgttcc acagagcgac
    19381 agagcagacc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc gtgcttagat
    19441 caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg atggtatcgc
    19501 aaggaaacga accgcacggg caatgtcacg ggtgcgtggc gcgggcggca gactggcgag
    19561 aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaattacca gcacgccgcg
    19621 atcgtcggcg tcgcgaatca gtcgccctac tccctgcttg agagtaatga ccgcatccgg
    19681 cagctgcact tcatcgaacg ggtcgccacc gcgcaaacga caatcttcca tgcgcgcttt
    19741 taacagtaga tcatccggcg aggtaaacgg caatttgtcg ataattacca atgacaatgt
    19801 atcgccacgc acgtccaccc cttcccagaa actactggtt gccacaagaa gcgcgttacc
    19861 ggcgctgaca aattgctgca acagttgccc tttgctggtt tccccctgca acaaaacggg
    19921 aagcgtcatg gtagcgcgga actgctcggc cagatcgcgc atcatggcat gcgaggtaca
    19981 aagcataaaa caacgaccgt tgttagcttc gatgatcggt cgcagcattg ccgccagttg
    20041 ccgtgcacaa cccggttgat tggtttgcgg caggttgcgc ggcacacaga gtaacgcctg
    20101 gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgttcta tcccaagccg
    20161 cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt gctgaggtga agatccagct
    20221 accgggcttt tgcgccatta actctttgaa tttatccgcc acgctgagcg gcgtgagagc
    20281 cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct gattgatctc
    20341 ttttagccgc ttcagccgtg tgcgatacaa cgtggcgcgc tcaaatgccg catccagcaa
    20401 ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg tgtcatcgag
    20461 cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt taccgcgata
    20521 acctggctca ccgagttgca gacgaaaatc ctgtgcactc tgggcaagac gatcagcgca
    20581 cttttgtaac tgctgggtgt cttttaattc ggtgcggtag gcgatggtga tgtcttttgc
    20641 caggtcgagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg caatgtccgg
    20701 tagctggtgg gcttcgtcga agatcatgac gtccgcttca gggatcaact cgccaaatcc
    20761 actctcttta accaccatat ccgccagaaa gagatgatgg tttaccacca ccacatcggc
    20821 gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg ggcagtcgct
    20881 gccaagacag ttgtcgttgg tgctggtgac cagcggccac gcctgtgaat cttccgccac
    20941 gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc gcagcaggat
    21001 cacatcgctt aagatttgta ccggcagatc gccccccgcc agcgcctgct gttcgagacg
    21061 ttcgaggcag aggtagtttg agcgcccttt cagcagcgcc acgttgcccg tatatttcaa
    21121 tgcctttgag actgttggca aatcgcggct gtagagctga tcctgcaacg cttttgagcc
    21181 ggtcgagata atgactttct ttttcgcccg cagcgcagga gccaggtaag cgtaggtttt
    21241 gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg cctgggtgac
    21301 ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg ctttcgccag
    21361 ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga acagggatta
    21421 tgtcaggatg agggcgcttt cgccagttga agtggtgacg gcaacctcac attgtggcag
    21481 tctttgcagc acgaaaatgg aagtataatg aggacaaaat gatgactatc gttcgtatcg
    21541 atgctgaagc ccgctggtct gatgtagtaa tccacaacaa cacgctctac tacactggtg
    21601 taccggaaaa cctcgacgcc gatgcctttg agcaaaccgc caacacgctg gcacagattg
    21661 acgccgtgct ggaaaaacag ggcagcaata aatcgagcat tctggatgcc accattttcc
    21721 tggccgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg gttgtcgcgg
    21781 gtcatgcgcc agtgcgctgc acggtacaag cgggtttgat gaacccgaag tataaagttg
    21841 aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca gacgataatt
    21901 tatcgataat actggtcggt tttacataaa tcgaccagag aataagatat tactgttgat
    21961 gcttctgcta attcattatt tatatttata atttcaattt tatctataaa actaactata
    22021 taaataaaca taaccatgcc gttgtgttcg ccctttgatt agcaactctg gttttctttc
    22081 ttagctgaac aatctggtta tagcgtgcgg actgaccgtt aattttctcc ttcttactgg
    22141 ataacacacc atgagctgct acctttaaaa cacctgaatg tcaacaggtt aactcgtgcc
    22201 atatcgttat aatccctttg atattagatg caaatta
//
LOCUS       DAQSWQ010000066        21995 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.066, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000066 DAQSWQ010000000
VERSION     DAQSWQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.066"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="R8G18_002649"
     CDS             complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="R8G18_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HEE9796306.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="R8G18_002650"
     CDS             complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="R8G18_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HEE9796307.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="R8G18_002651"
     CDS             complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="R8G18_002651"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEE9796308.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4040..4798
                     /gene="loiP"
                     /locus_tag="R8G18_002652"
                     /gene_synonym="yggG"
     CDS             4040..4798
                     /gene="loiP"
                     /locus_tag="R8G18_002652"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HEE9796309.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4941..5861)
                     /gene="speB"
                     /locus_tag="R8G18_002653"
     CDS             complement(4941..5861)
                     /gene="speB"
                     /locus_tag="R8G18_002653"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEE9796310.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5999..7975)
                     /gene="speA"
                     /locus_tag="R8G18_002654"
     CDS             complement(5999..7975)
                     /gene="speA"
                     /locus_tag="R8G18_002654"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEE9796311.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="R8G18_002655"
     CDS             complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="R8G18_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HEE9796312.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8251..8466
                     /locus_tag="R8G18_002656"
     CDS             8251..8466
                     /locus_tag="R8G18_002656"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796313.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8770..9924
                     /gene="metK"
                     /locus_tag="R8G18_002657"
     CDS             8770..9924
                     /gene="metK"
                     /locus_tag="R8G18_002657"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEE9796314.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10360..11754
                     /gene="galP"
                     /locus_tag="R8G18_002658"
     CDS             10360..11754
                     /gene="galP"
                     /locus_tag="R8G18_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HEE9796315.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11831..12328
                     /locus_tag="R8G18_002659"
     CDS             11831..12328
                     /locus_tag="R8G18_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEE9796316.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12423..13130
                     /gene="endA"
                     /locus_tag="R8G18_002660"
     CDS             12423..13130
                     /gene="endA"
                     /locus_tag="R8G18_002660"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HEE9796317.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13210..13941
                     /gene="rsmE"
                     /locus_tag="R8G18_002661"
     CDS             13210..13941
                     /gene="rsmE"
                     /locus_tag="R8G18_002661"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEE9796318.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13954..14904
                     /gene="gshB"
                     /locus_tag="R8G18_002662"
     CDS             13954..14904
                     /gene="gshB"
                     /locus_tag="R8G18_002662"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEE9796319.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15013..15576
                     /locus_tag="R8G18_002663"
     CDS             15013..15576
                     /locus_tag="R8G18_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEE9796320.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15576..15992
                     /gene="ruvX"
                     /locus_tag="R8G18_002664"
     CDS             15576..15992
                     /gene="ruvX"
                     /locus_tag="R8G18_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEE9796321.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16167..17147)
                     /locus_tag="R8G18_002665"
     CDS             complement(16167..17147)
                     /locus_tag="R8G18_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEE9796322.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17165..17869
                     /gene="yggS"
                     /locus_tag="R8G18_002666"
     CDS             17165..17869
                     /gene="yggS"
                     /locus_tag="R8G18_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HEE9796323.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17887..18453
                     /gene="yggT"
                     /locus_tag="R8G18_002667"
     CDS             17887..18453
                     /gene="yggT"
                     /locus_tag="R8G18_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HEE9796324.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18450..18740
                     /locus_tag="R8G18_002668"
     CDS             18450..18740
                     /locus_tag="R8G18_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HEE9796325.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18748..19341
                     /locus_tag="R8G18_002669"
     CDS             18748..19341
                     /locus_tag="R8G18_002669"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEE9796326.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19334..20470
                     /gene="hemW"
                     /locus_tag="R8G18_002670"
     CDS             19334..20470
                     /gene="hemW"
                     /locus_tag="R8G18_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEE9796327.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20781..21767
                     /locus_tag="R8G18_002671"
     CDS             20781..21767
                     /locus_tag="R8G18_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEE9796328.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            21812..>21995
                     /locus_tag="R8G18_002672"
     CDS             21812..>21995
                     /locus_tag="R8G18_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEE9796329.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
BASE COUNT         5441 a         5718 c         5597 g         5239 t
ORIGIN      
        1 gcaaaggaca ccagggtagc taccgtaaca ccggcaattg tcgccaggaa cgagcctttc
       61 ggcgtcagtg ccaggtaagc aaagatagac cccggacttg ggccagccac cagaccaccg
      121 tccagtaagt taaacaccca ggtgccagac ataccgcccg caatcatggc aataatggtc
      181 agcggcttca tcagcacata cgggaaatac agttcgtgga tcccaccgag gaagtgaata
      241 atcatcgccc cgggcgcaga acgtttactc atccctttac caaacaaggt aaacgccagc
      301 agcagcccta agccaggccc aggattggac gccaccataa agaagatgga tttaccgtta
      361 accgaagcct gttgcattcc cagcggataa tagacgccct gatcgatcgc attattgagg
      421 aaaagcactt tcgccggttc attgatgact gacagcagag gcagataacc cgcatgtacc
      481 agcgcctcaa tacactcttt gacgagagta ttggcaatta acaccgccgg gccgataact
      541 tcaaaaccca gcagacaaag gagcatcccc gcgataccta atgagaagtt attgataacc
      601 atctcaaaac cggcaggtat gcgtttttcc agtgccttat caacatattt tatgaccagc
      661 ccaccgagcg gccccataat cattgagcca aggaacatcg ggatctctgc gccgacgatc
      721 acaccaatag tacctattcc gcccatgacc gcgccgcgtt taccgccgac cagatgacca
      781 ccggtagaac caatcatcac gggcaataaa taggtaatca tcgggccgac aattttggcg
      841 aaatgttcgt taggcagcca accggtggga ataaataacg cagtaataaa accccaggca
      901 ataaaagcac caatattggg gatgaccatt gccgtcaaaa agcccccaaa agcctggacc
      961 tttgcacgag cagacttgtt ttccataata ttatcctgta gaggagagag taattatccg
     1021 cggctgatta tgtccgctaa ttgtttttct gatgctgctg ttaagagttg ttcgagtatt
     1081 tcttcttcgc acagtaattc acttaacgcc tgaatagcgc caatgtgcga atcggcatcg
     1141 gcagcagaaa ggccgatgag taattttatt ggttcgtcat tccccggaaa ataaacacct
     1201 tgttcaagta atgtcaaaga catcccggtt ttaagcgccc cacattccgg tcgcgcatga
     1261 ggcattgcca cgcctggtgc gagaatataa taagggccat tgttaatggt ggaatcttta
     1321 attgcctgaa tgtaattctc gctgatatag tttttatcca gcaatgatac catcgagaaa
     1381 tcgatagctt cctgccaatc ttttgctgaa tgtataactg agattgatga ttctggaaaa
     1441 tagtcactaa gccgcataaa ttatccttat tttattttta gggtaccgtc cggcggtaaa
     1501 aataaaatac tcgtgcggtt taatgaaagc aattgagcag ggaaaccggt gttttgaaaa
     1561 cagtgaatta ttaatttctt atataacatt gagttataga tataacaaca aatgtcaata
     1621 cgcatatcgt gatgcatatt acataattga tatttattgc atataaatat ttgtgatcta
     1681 caacacgctt tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg
     1741 ccgaagcgac ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacgac
     1801 gttgtcgacg gtgaagccga actcttcgaa cagcaactct gccggagctg actcaccgaa
     1861 ggtggtcata ccgatgatag cgccattcag gccaacatac ttgtaccagt agtcagcaat
     1921 acccgcttct accgcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc
     1981 cgcatcctgc ttgtcgaatg catcggtaga cggcatggaa accacgcgcg ccttcacgcc
     2041 ttcggcagtc agtttttcgt aggcggcaac agccagttca acttccgaac cggtcgcgat
     2101 gaagatcagt tccggctgac cagcacagtc tttcagcaca taaccaccgc gcgcgatgtt
     2161 tgccagttgc tcttcagtac gttcctgctg cgccagattc tgacgggaga ggataagcgc
     2221 ggtcggaccg tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc
     2281 acacggacgc catgtagaca tgttcggggt cacgcgcaga gaagcgacct gctcaaccgg
     2341 ctggtgagtc gggccgtctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg
     2401 acgctgtttc atcagcgcag ccatacgtac agcgttacgt gcgtattcga caaacatcag
     2461 gaaggtggag gtgtacggca ggaagccacc gtgcagggag ataccgttag caatcgcggt
     2521 cataccgaac tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc
     2581 tttagaacca gaccacaggg tcaggttaga cggtgccagg tcagcggagc cgccgaggaa
     2641 ttccggcaac agcgggccga acgcttcaat agcattctga gacgctttac ggctggcgat
     2701 tttcgccgga ttagcctgca gtttagcgat aaactctttc gctttggcgt cgaagtcaga
     2761 cggcatttcg cctttcatac ggcgggtaaa ttcagccgct tcctgcggat aagctttcgc
     2821 gtaagccgca aacttctcat tccatgcaga ttctttcgcc tggcctgctt ctttcgcatc
     2881 ccactgcgca tagatttcag acgggatttc gaacggtgcg tatttccagc ccagctgttc
     2941 gcgggtcaga gcgatttctg catcacccag cggcgcaccg tgggagtcgt gagtaccggc
     3001 tttgttcggg gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt
     3061 cactgcgcgc gcttcttcta ctgcgcgttt gatggatgct gcgtcatgac cgtcgatgtc
     3121 gcgaataacg tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc
     3181 ttcaacgtga ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag
     3241 cttcagcgta cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc
     3301 gcccatgaag gcgtaggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc
     3361 cgccagcgtt ttttctgcaa tcgccatacc gactgcgttg gcaataccct gacccagcgg
     3421 accggtggtg gtttccacac cagcggtata acctacttcc gggtggcccg gagttttaga
     3481 gtgcagctga cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag
     3541 caggctgtag atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc
     3601 ccaggacgga ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc
     3661 agccataccc ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct
     3721 cagcgcacga atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat
     3781 gatgaagggc acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt
     3841 tgccaacaat ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag
     3901 aaatctctgg atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc
     3961 ttatactgag actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac
     4021 cttgtgcgga agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac
     4081 ggtactgact ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc
     4141 ttttcaggct tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca
     4201 ggagatggac agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac
     4261 aactattgcc aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta
     4321 tatggcgaag gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg
     4381 gctgatggat atgatgacag ataacgaagt cgaagcggtg atcggtcacg agatggggca
     4441 cgtggcgtta ggccatgtga agaaagggat gcaggtggcg cttggtacaa atgccgtgcg
     4501 agtagctgcg gcctctgcgg gcgggattgt cggcagttta tctcaatcac aacttggtga
     4561 tctgggcgag aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga
     4621 ttattcttac gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt
     4681 tgaaaaactg gcgaaactgg aagaaggccg ccaaagctca atgtttgacg accatccggc
     4741 atccgccgaa cgcgcccagc atattcgcga tcgcatgagc gcggatggga ttaagtaaag
     4801 cctggtggtg tcgaacgtag gtcagataag gcgttcacgc cgcatctgac atcaatggca
     4861 catttgttac cctgtgcgca ttgccggatg cgatgctgac tgcatcttat ccggcctacg
     4921 ggtggcatcg catctggtgc ttactcgccc tttttcgccg cttggatata cagcatctcc
     4981 agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact
     5041 tccactacgt ccatcccaac aatgttgaga tctttcaggc cgcgtaccag tttaatagcg
     5101 cgatcggagg tcaggccgcc aatcactggc gtaccggtgc ctggtgcaaa agcaggatcc
     5161 aggcagtcga tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacttgg
     5221 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
     5281 ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
     5341 ccttctttcg gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag
     5401 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
     5461 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg tgagcatacg cttaccggca
     5521 gccagcagct tctcggcgtg cgcctgcagc ttttcgctca tctcacgggc atcgccaaag
     5581 gcatatacca gatcgccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac
     5641 gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg
     5701 tggcgaccac ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag
     5761 tctgcatcgc tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg
     5821 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
     5881 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgtc
     5941 acaataaatt gtggcggatt atcaccgcta aattaagcgg attcaagtaa cacaggactt
     6001 actcatcttc aagataagta taaccgtaca aacctgcctc gaactcttca aggaactgtt
     6061 gttgcagttc agcatcaaga tcggttttct tcacttgatc gcggaactgg gttaacagcg
     6121 ttttcggatc gagctgtaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca
     6181 gttctacttc tacgctaccg tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga
     6241 acaggttgtg catattgcca aggatctcct gatatgcgcc gaccataaag aaaccgagca
     6301 tcggcggatt ctctggatcg tactccggca ttggcattgt cgtggcaata ccatcaccat
     6361 caatatagtg gtcgatagca ccgtcagagt cacaggtaat atccagcagc acagcgcgac
     6421 gttccggcac ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc
     6481 atgcgtccgg cattgactgg aacagcgaga aattgacgta cattttgtcc gccatacgtt
     6541 cctgcagttc gtcgattatc ggacgatgcg cacggttttg cggatccagt tgcttctgca
     6601 cttcatggca catgctcaga tagagttgtt ctgcccatgc acgttcttgc aggctaaagg
     6661 tgccggaaga gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt
     6721 cacgcaaaga acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt
     6781 gcagcgcgcg cggcgcatct tctgcgggcg cggtcggcac cgtatattcg ttacgttcca
     6841 cgccgatgat attcgacacc agcacggtat ggtgcgcagt caccgcacgg cccgattcgg
     6901 tgattaccgt cggatgcggc agaccatttt cttcacacgc atcgccaatc gcccagatga
     6961 tgttgttggc gtattcattg aggccatagt ttacggagca gtcagactgc gaacgggtgc
     7021 cttcatagtc cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct
     7081 tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc cgtggcgata tcgcgaatat
     7141 tcgccatctg cgaaccgagg tggaagtgca gtagttgcag gctgtcgaga cgcccggctt
     7201 cacgcagggt ttcaaccagt tgcagtacct gagtcgcagc caggccgaac ttcgattttt
     7261 ccccgccgga ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca
     7321 gacgaggaac gacattcaga cgttctgctt catccagcac aatggcgatt tctgacatct
     7381 tctcaatgac cagatagacc ttgtgcccca tcttctcgcc aattaatgcc aggcggatat
     7441 attcgcggtc tttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca
     7501 gtactgccat caactcggct ttggaaccgg cttccagacc cagcggttcg cccgaatgaa
     7561 tcagggactc aatcacgcgg cggtgctggt taactttgat cggataaaca aggaagtaat
     7621 cgccgttata gccgtaggat tccctcgcac gtttgaacgc ggcgttaatg gaacgcaaac
     7681 ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac
     7741 gagttttcac taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca
     7801 cgctgatgtg gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt
     7861 aagtacgcag catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta
     7921 gtacaccgtg ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga
     7981 acctcaaatt attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga
     8041 taacaaccca taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac
     8101 cggtttcttt ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg
     8161 aaaccacgag aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtaactgta
     8221 accagctctt gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc
     8281 agagatgcag agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag
     8341 acaacggttc attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc
     8401 aaaactctga tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt
     8461 atttaagccg cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata
     8521 cgcagaatgc cggcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac
     8581 gattcaaaaa aaagtggaaa tcgggtgaag aattgaccta aaatagccat ccagatgtta
     8641 atccatccat accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta
     8701 ggatccgcta ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg
     8761 atattaaata tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac
     8821 aaaattgctg accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa
     8881 gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc
     8941 accaccagcg cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc
     9001 tatgtgcatt ccgacatggg ctttgacgct aactcctgtg cggttctgag cgctatcggc
     9061 aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg
     9121 ggtgaccagg gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca
     9181 cctatcacct atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact
     9241 ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa
     9301 atcgttggta tcgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa
     9361 tcgctgcaag aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg
     9421 acttctgcca ccaaattctt catcaacccg accggtcgtt ttgttatcgg tggcccgatg
     9481 ggtgactgcg gtctgactgg tcgtaaaatt atcgttgata cctacggcgg catggcacgt
     9541 cacggtggcg gagcattctc tggtaaagat ccatcaaaag tggaccgttc cgcagcctac
     9601 gcagcacgtt atgtcgcgaa aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt
     9661 caggtttcct acgcaatcgg cgtggctgaa ccgacctcca ttatggtaga aactttcggt
     9721 actgagaaag tgccttctga acaactgact ctgctggtac gtgagttctt cgacctgcgc
     9781 ccatacggtc tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca
     9841 tacggtcact ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg
     9901 cgcgatgctg ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc
     9961 tggccttttt cttttacctc accgcggtgg ataggcattc acgccgcatc cggcaaaaag
    10021 ctgcccgcac aataacatca ttcttcctca acacgtttta tcgcaaatta tcatcactac
    10081 tgaaaccgat tacaccaata acaccagcca aatatttgta atattttcat attattattc
    10141 agttttcaca gttgttacat ttcttttcag taaagtctta attgcagata acagcgttta
    10201 atctatgatg atataactca attattttca tgcacttaaa ccataactaa gataaatgtt
    10261 agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt actcacctat
    10321 cttaattcac aataaaaaat aaccatattg gagggcatca tgcctgacgc taaaaaacag
    10381 gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta
    10441 ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc
    10501 cagattactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc
    10561 ggtgcggtgg gcagcggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc
    10621 ggcgcaattt tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa cgttgaagta
    10681 ctgattcttt cccgcgttct actggggctg gcggtgggtg tggcctctta taccgcaccg
    10741 ctgtacctct ctgaaattgc gccggaaaaa attcgtggca gtatgatctc gatgtatcag
    10801 ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc
    10861 ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt
    10921 gtcttcttcc tgccagacag cccacgttgg tttgccgcca aacgccgttt tgttgatgcc
    10981 gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa
    11041 atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaacagcaac
    11101 ttccgccgcg cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg
    11161 aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacaccaca
    11221 gagcaaatgt gggggacagt gattgtcggc ctgaccaacg tacttgccac ctttatcgca
    11281 atcggccttg ttgaccgctg gggacgaaaa ccaacgctaa cgctgggctt cctggtgatg
    11341 gctgctggca tgggcgtact cggtacaatg atgcatatcg gtatccactc tccgtcggcg
    11401 cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt
    11461 ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc
    11521 acctgctcca ctgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc
    11581 atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg
    11641 tttatcctgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt
    11701 gaacgtaacc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct
    11761 caggcttcct cccgtcgcgg aggaagccgc ctcttgcagt catcttttct tcgctctatc
    11821 ctctgccgct atgaaaacat cccgtctccc tatcgccatc caacaggccg ttatgcgcgg
    11881 cctgcgggaa aaactcgccc aggccaacct gaagctaggg cgtaactacc cggagccaaa
    11941 actctcttac acccagcgcg gaacctccgc cggaacagcc tggctggaaa gctatgaaat
    12001 ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct tttattgaag aagtggtacc
    12061 gcacgaactg gcacatttgc tggtatggaa acatttcggc cgcgtagcgc cacatggcaa
    12121 agagtggaag tggatgatgg aaagcgtgct gggtgttccc gcccgtcgta cgcatcagtt
    12181 cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc aggagcatca
    12241 gcttaccgta cgccgccata atcgcgtagt tcgtggcgag gccgtctatc gctgtgttca
    12301 ctgcggtgaa cagctggttg cgaaataacc atctgaacta tcaggaactt tcctgatctg
    12361 gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac acggagtaag
    12421 tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt tccggcccgg
    12481 cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta aaagtccacg
    12541 ctgacgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg
    12601 ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccga gccagccgcg
    12661 tagagtggga acacgtcgtt cccgcctggc agttcggtca ccagcgccag tgctggcagg
    12721 acggtggacg taaaaactgc gctaaagatc cggtctatcg caagatggaa agcgatatgc
    12781 ataacctgca gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt atgtacagcc
    12841 agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa
    12901 aagctgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc
    12961 gcgaccaata caacctgaca ctctctcgcc agcaaacgca gctgttcaac gcatggaaca
    13021 agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg aaggtgcaag
    13081 gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa cctacactag
    13141 cgggattctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag acgacgcgga
    13201 tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag ccattctcac
    13261 atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat ggggccgggg
    13321 caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat taccagcgcc
    13381 agcaaaaaaa gcgtggaagt gaaggtgctg gaaggcaaga tcgacgatcg cgaatctccg
    13441 ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag
    13501 aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg ctgcggcgtt
    13561 aaactggata gtgaacgtct gaacaagaag ctccagcagt ggcagaagat tgcaattgct
    13621 gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat ggatctggaa
    13681 gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg cgccagtaac
    13741 agcatcaata cgttgccgtt accggttgaa cgcgtccgcc tgctgattgg cccggaaggc
    13801 ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga tatcctgttg
    13861 ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc gctacaagta
    13921 cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac
    13981 cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag
    14041 cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat caatggcgaa
    14101 gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg gttttcgttc
    14161 gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg
    14221 ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg
    14281 acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact gtttaccgcc
    14341 tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca gctaaaagcg
    14401 ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg
    14461 attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac cctgactgag
    14521 catggcactc gctactgcat ggcacaaaat tacctgccag ccattaaaga tggcgacaaa
    14581 cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat tccgcagggg
    14641 ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa
    14701 agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg gctgattttt
    14761 gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt
    14821 attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc
    14881 gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga
    14941 gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca
    15001 gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat
    15061 ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg
    15121 atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag
    15181 atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggcggt
    15241 ccgctggctg aagatcgcgg gtttattttg catactccgc cctccaattt tgcttccagc
    15301 attcgcattt cagacaacac agtgatgacc acttcccgcg atgtgctgga aacgctcggc
    15361 accgataaac aaccgtctga cgtattggtg gctctaggtt atgcctcctg ggaaaaaggt
    15421 caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcgga tctgaatatt
    15481 ctgttcaaaa cgccgattgc cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat
    15541 attctcacca tgcctggtgt ggcagggcac gcctgatgag tggaacctta ctcgccttcg
    15601 acttcggcac caaaagcatt ggcgtagcgg tcggccaacg cattaccggc actgctcgcc
    15661 ctttgcctgc aattaaagca caggacggta cgccggactg gaacctcatc gaacgtttac
    15721 tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg
    15781 agcaaccatt gactgccaga gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg
    15841 ttgaagtaaa gctccatgac gaacgtctta gcaccgtgga agcccgttcc ggtctgtttg
    15901 aacagggcgg ctatcgggcg ctcaacaaag gcaaagtcga ctctgcctct gcggttatta
    15961 ttctcgaaag ctatttcgag cagggatatt aaggcgattt aaaagcctgg cggatagtcg
    16021 ttaccgccat cttgccggat gcggcgtgaa cgcctatccg gcctacatgt ggcagcgatt
    16081 gtaggtctga taagacgcgc aaacgtctca tcagacgttt attgcctttg taggcccgat
    16141 aagcttgcac atcgggcatg gcaacgtcac aaacgccctt cccccactcg ctgctgaaaa
    16201 ctctgctgaa acgttatcat ccccacctgc tgcccggttt gaataacatg cggtaactgg
    16261 tgggttttcc cttcgcgaat caaattcccc accgcgggag tgttaatcaa taattcaaac
    16321 agcgccacgc gtccttcctg tttatccact tccagctttt gtgacaacac ggcccgtaaa
    16381 ctacctgcca gttgattacg tacgggatct ttttcctgcg ccggaaatga atccaccagt
    16441 cgctcaactg cctgcgcggc accacgcgta tgtaatgttg ccagcaccaa atgcccggtt
    16501 tctgccgccg tcagtgccag acggattgtc tcgctgtcac gcagctctcc gagcaaaatc
    16561 acatcaggat cttcccgcaa tgcggcccgc aatcccgatg cgaacgtcat acagtgcaaa
    16621 ccaatttccc gcttctggat caaacatcgc tggctggcat agatatattc aacaggatct
    16681 tccagcgtca gaatatgcgc atcggcatgt tgattgagat agccaaccat cgccgccagc
    16741 gtggtagatt tgctgctccc cgtcgccccc gtcaccagaa tcaggccatt ctcactcttg
    16801 agtaattccg gcaataccgt tggtgcgcca agctgttcga gctgcgggca gtgcgaaggt
    16861 aacagccgta acgccagcga aatgccatgc cgttgcgcaa atgcgctgcc acgcaatcgc
    16921 tggttttccg ccagcgacac ggcaaaatcc agctggccat tttccagcaa tattgcccgc
    16981 tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt ccggcgcctc aaacggcgca
    17041 gcttccatta gcccgcgaat gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc
    17101 gagacgttat gctttacact aagggccaca atttcttcca tattcatact aagatcctcg
    17161 gaaaatgaac gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc
    17221 aacgcgttgc ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc
    17281 tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt
    17341 tcaggaaggg gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg
    17401 gcattttatt ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg
    17461 gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc aacgatcagc gcccggcaga
    17521 acttccccct cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg
    17581 gattcaactg gcagaactgg acgagctggc agcggcggtc gctgaactac cgcgtttacg
    17641 tctgcgcggg ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt
    17701 tgcacgccaa atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac
    17761 gctctctctg ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt
    17821 tcgtatcggc actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg
    17881 aacgccatga atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg
    17941 ctgttattac gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag
    18001 tttgtagtga aggtaacgca gccaattatc gggccactgc gccgcgttat tccggcaatg
    18061 ggccccattg acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatc
    18121 gtgctgttta aagtggtgac cttcctgcca atcatctgga ttgctggttt actgattctg
    18181 ctgaaaacca tcggcctgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg
    18241 gtaagccagg gtcgtagtcc gattgaatac gtgctgattc agctggccga tccgctgctg
    18301 cgcccgattc gccgcctgct accggcaatg ggtgggattg atttctcgcc gatgatcctc
    18361 gttctgctgc tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac
    18421 atgctgctgc cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc
    18481 tggttttacg gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg
    18541 gcgacgaagt taaagtcgcc attaccgcgc cgccggttga cggccagggc aacagtcatc
    18601 tggtgaagtt tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag
    18661 gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa
    18721 tcgcggcgtt aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc
    18781 ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa
    18841 acagacctcg gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt
    18901 ctgaaagcgc gctatgcggc aaaagtgacc ggtttaccgg caattgccga cgactctggt
    18961 ctggcggtag atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa
    19021 gacgcgaccg atcaaaagaa tctgcaaaaa ctgttggaaa cactgaaaga cgtaccggac
    19081 gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg
    19141 actccgctgg tatgccacgg cagctggccg ggcgtgatta ctcgcgaacc agcgggcact
    19201 ggtggctttg gttatgatcc aatcttcttc gtaccttccg aagggaaaac cgccgccgaa
    19261 ctgacccgcg aagaaaagag cgccatttcc caccgtggtc aggcgttgaa actgctgctg
    19321 gacgctttac gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg
    19381 cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca
    19441 cgacgattat gttcagcatc tgctttgcga tctggacaat gatgtggctt acgctcaggg
    19501 ccgtgaagta aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc
    19561 gatgcaaacg ctgctggacg gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat
    19621 tactatggaa gcgaaccctg gaacggtaga agccgatcgc tttgtcgatt atcagcgtgc
    19681 tggtgtgaac cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact
    19741 tgggcgtatt catggcccgc aagaagcgaa acgcgcggca aagctggcga gcgggctggg
    19801 gctgcgtagc tttaacctcg atttgatgca tggactaccg gatcaatcac tggaagaggc
    19861 gcttggcgat ctacgccagg ccattgaact gaatccgccg catctttcct ggtatcaact
    19921 gaccatcgaa cccaatacgc tgtttggttc gcgaccaccg gtgctgccgg acgatgacgc
    19981 gctgtgggat attttcgaac aggggcatca gttattaacc gcagcgggtt atcagcaata
    20041 tgaaacttcc gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg
    20101 atttggtgac tacatcggta ttggctgcgg cgcgcacggc aaagtcacct tcccggatgg
    20161 gcgcattctg cgtaccacta aaacgcgtca tcctcgtggt tttatgcaag gaaggtatct
    20221 ggaaagccag cgtgatgtcg aagccgcaga taagccgttt gagttcttta tgaatcgctt
    20281 ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga
    20341 tgtgattcgc ccacagttag acgaggcgat tgcccagggt tatctcaccg aatgtgcgga
    20401 ttactggcag ataaccgagc acggtaaatt attcttaaac tcacttcttg aattattcct
    20461 cgctgagtaa tcattaatgc gcctgcgggc gcattaatca cttcttttcc ccccgttaat
    20521 taacttcggc aaaatatatt tcttactgaa taaacatcga tgatatttat tagatatatc
    20581 tagtgaatat ttactcatca tttattgatt ataaacatgt acaacctggc gcacaaattt
    20641 aatatttaat ttctgacttt atttcaagtt gtgataaaca tagacaagtt gtcagacagg
    20701 ttaaccaatt tgtcgcacaa agcactgata agcacaatta gatatcaacc aatttccatc
    20761 cttcaacagg gagttttttt atgaaaatga ggtcatttac ccgctcatta gtctgcgcat
    20821 ccttgctggc tctggttagc actggcgtaa acgccgcaga aaaagtgacg ttaaagttag
    20881 cacacaacct ggaaagaagt catgttgtac atcagagttt tgaagagctg gcgaaagagg
    20941 taaaacagct gtcagaaggt aatatggtga tccgtattta tcccagcagc cagatgggta
    21001 atgcgcgaga aactatggaa ttgttgcaaa atggcgcact ggatatgacc aaaggttccg
    21061 ccagtgatct ggagtctttt gataatattt atgctatcta taatttgccg ttcttattta
    21121 aagatcaggc tcacttcaac aaagttgttt ttggtgaagt tggtaaagaa attatggact
    21181 caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtaca cgcagtttct
    21241 atgcgaaaaa accgattact aagccagaag atcttaaagg gctgaaaatt cgtgttcagc
    21301 caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact ccaatttcat
    21361 ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa aataacgtgc
    21421 cttcctgggt gcagactcga catattgaaa ttgccaaagt tttttctgaa gatgaacacg
    21481 cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta accccagaac
    21541 agaaacaaat cctcgaaaca gcagcgaaga aatccgaagc ttatcaacaa aaattgtggg
    21601 aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa attgtaaaag
    21661 tggataaagc accattccgt gccgcagttc agcctctgtt tgacgacttc aaaaaagatc
    21721 caaaacaagc tgctttgtta gagaagttcg acaacgctgc tcaataatat tccgggccag
    21781 tctctggccc tgattttcca agatgcttac aatgatattt aaccgcctga aacttgcggt
    21841 agaccgcgtc attgccgcgt tttctgtcgc cgtcatgctt gctctggttg tatgcgtagt
    21901 ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg atgagctggc
    21961 acgatttttg atgatctggg tagggttatt aggtg
//
LOCUS       DAQSWQ010000067        21983 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.067, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000067 DAQSWQ010000000
VERSION     DAQSWQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.067"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(121..330)
                     /locus_tag="R8G18_002673"
                     /pseudo
     CDS             complement(121..330)
                     /locus_tag="R8G18_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="R8G18_002674"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="R8G18_002674"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEE9796330.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="R8G18_002675"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="R8G18_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEE9796331.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="R8G18_002676"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="R8G18_002676"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HEE9796332.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="R8G18_002677"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="R8G18_002677"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEE9796333.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="R8G18_002678"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="R8G18_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HEE9796334.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="R8G18_002679"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="R8G18_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HEE9796335.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="R8G18_002680"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="R8G18_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEE9796336.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="R8G18_002681"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="R8G18_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEE9796337.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10406)
                     /gene="motB"
                     /locus_tag="R8G18_002682"
     CDS             complement(9468..10406)
                     /gene="motB"
                     /locus_tag="R8G18_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEE9796338.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELS
                     ADRANASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQA
                     ILHENAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10403..11290)
                     /gene="motA"
                     /locus_tag="R8G18_002683"
     CDS             complement(10403..11290)
                     /gene="motA"
                     /locus_tag="R8G18_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEE9796339.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11416..11994)
                     /gene="flhC"
                     /locus_tag="R8G18_002684"
     CDS             complement(11416..11994)
                     /gene="flhC"
                     /locus_tag="R8G18_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HEE9796340.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11997..12347)
                     /gene="flhD"
                     /locus_tag="R8G18_002685"
     CDS             complement(11997..12347)
                     /gene="flhD"
                     /locus_tag="R8G18_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEE9796341.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13127..13555
                     /gene="uspC"
                     /locus_tag="R8G18_002686"
     CDS             13127..13555
                     /gene="uspC"
                     /locus_tag="R8G18_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HEE9796342.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13562..14986)
                     /gene="otsA"
                     /locus_tag="R8G18_002687"
     CDS             complement(13562..14986)
                     /gene="otsA"
                     /locus_tag="R8G18_002687"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HEE9796343.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(14961..15761)
                     /gene="otsB"
                     /locus_tag="R8G18_002688"
     CDS             complement(14961..15761)
                     /gene="otsB"
                     /locus_tag="R8G18_002688"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEE9796344.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(15928..16914)
                     /gene="araH"
                     /locus_tag="R8G18_002689"
     CDS             complement(15928..16914)
                     /gene="araH"
                     /locus_tag="R8G18_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HEE9796345.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(16929..18443)
                     /gene="araG"
                     /locus_tag="R8G18_002690"
     CDS             complement(16929..18443)
                     /gene="araG"
                     /locus_tag="R8G18_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HEE9796346.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(18513..19502)
                     /gene="araF"
                     /locus_tag="R8G18_002691"
     CDS             complement(18513..19502)
                     /gene="araF"
                     /locus_tag="R8G18_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HEE9796347.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            20299..20802
                     /locus_tag="R8G18_002692"
     CDS             20299..20802
                     /locus_tag="R8G18_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HEE9796348.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(20881..21132)
                     /locus_tag="R8G18_002693"
     CDS             complement(20881..21132)
                     /locus_tag="R8G18_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HEE9796349.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLNVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(21247..21333)
                     /gene="azuC"
                     /locus_tag="R8G18_002694"
     CDS             complement(21247..21333)
                     /gene="azuC"
                     /locus_tag="R8G18_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HEE9796350.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            21596..21919
                     /locus_tag="R8G18_002695"
     CDS             21596..21919
                     /locus_tag="R8G18_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HEE9796351.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         5373 a         5842 c         5180 g         5588 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca gaatgtcgcg catatagggt
     9901 tcgacatcgg cactgccggt tctaaacatc gggcgattct ggctatcgat gatctgaata
     9961 cgtagacctt cctggaccag atcgattttg agatggggac gtaacgcccg cagtttcgga
    10021 tcggactcta tcaactggtc gagatcaccc cgcaatttcc gcaatcgact ttgctccatg
    10081 cgttttttca gatcttcgat gttcggctgc ttattcactt ccccctggct ttgggtgtaa
    10141 tcatcaccac cgccgggaat tgggctttca ctattagaaa tgcgatcgcc gcccgtaacc
    10201 gcagtcgcca gtggagtccg gaagtactcc gcaatctgaa tcagctcttt tgggctggag
    10261 atggagatca gccacatcac cagaaaaaag gccatcatcg cagtcataaa gtcggcataa
    10321 gcaatcttcc acgatccatg tgctgccccg tggcttttgg ctttgcgtcg tttgacgaca
    10381 ataatcggat gcgcttgatt cttcatgctt cctcggttgt cgtctgttgt tgcggatttt
    10441 tcaccgcacg cacatgctct tccagttcaa taaacgacgg acgttcgctg gaatagagcg
    10501 ttttgcgacc aaactcaacg gcgataggcg gtgcgtaacc gttcagatta gaaagcagag
    10561 tgactttgac gcactgcatc attttgctgg tttcggcgct tttctgacgt aaaacactcg
    10621 ctaatgggga aataaatccg taagccaata aaatgccgag gaaagtcccc accatcgcat
    10681 gtgcgataag cgcacccagc tcggcggcag gacgatcggc tgaacctaac gcgtgaacgc
    10741 cccccattac agccgcgaca ataccaaacg ccggaagtga gtccccgacc agcgccagac
    10801 tgttcgccgg gacttctgcc tcgctttcgt gcgtctcaat ctcttcatcc atcagggctt
    10861 cgatttcgaa ggtgttcata tgaccgctga taatcagacg cagataatcg acgataaaat
    10921 caagcatgac gctatccgcg aggatgcgtg ggtagctggc gaagatctcg ctctcacggg
    10981 gattttcaat atcacgttcc agcgaaaaca tccccatctg ccgcgatttc gccatcaacc
    11041 gataaagcag agccagcaga tccatataca ttgctttggt gtatttggag cgacgaaaca
    11101 gcaacggcag cgccttcagc gtgcctttaa tcgctttgcc attattgccg acgataaacg
    11161 acccaatccc tgcaccggca ataatcacca gttcagcggg ttgatagagt gctccaaggc
    11221 ttccaccggt catcaaataa ccgccgaaaa ctgtaccgag aacaaccagg taacctaata
    11281 agataagcac gacatcatcc ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg
    11341 ttaacgcgcg attggggcaa aaaaagcagc ggtacgtcgt taccgctgct ggaatgttgc
    11401 gcctcaccgt atcagttaaa cagcctgtac tctctgttca tccagcagtt gtgggataat
    11461 atcggcagga ttctgggaaa gtttacgtct ttttactgcc cgggatggcg gttgacataa
    11521 gctgcaggca aagctgccaa caggctggtg agcgtgggta ataaaattgc cgccgcagca
    11581 gttgcagctg gaaagttgca gtaatccact ttcaacaaac cgcaccaatg tccaggcacg
    11641 ggttaatgcc agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt ataaacggta
    11701 ggctttgatc accgcatcga cgccattaca caaaccggtt ttcagtaaaa actgccatgc
    11761 attacagaac atcgaagcat gaacgttttg ttcccaggtc ataaaccagt cggttgagaa
    11821 tggcagcatg cctttcggcg gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg
    11881 tccgcgactt aactgtgttt cgctttccag catctgcaaa cgagcgccca gggtaatcaa
    11941 ttccattgcc agctgaatat cccgcgcttc ctgaacaatg cttttttcac tcatgatcag
    12001 gcccttttct tgcgcagcgc ttcttcaggc tgattaacat cattcagcaa gcgtgttgag
    12061 agcatgatgc cggtatgaat ttgctggaga tcgtcaacgc gggaatcttg cgtcaactga
    12121 gtaatcgtct ggtggctgtc aaaacggaag tgacaaacca gttgattggt ttctgccagc
    12181 ttaaccattt gcggaagagt cagtgccgct aacgttgtcg ccatttcttc atttatgccg
    12241 agacgaaaca tagcggacgc tttgtcctga acaatcaaac gctgtgcaag tagtaaatat
    12301 gacaagttga tgtcataaat gtgtttcagc aactcggagg tatgcattat tcccacccag
    12361 aataaccaac tttattttta tgcggtttca ccgcaccccg tgatgtcgcc gggaagcccc
    12421 ggtaaaaaat aattagcatt agaatagttg cgataagctg caataagcag aaccaccttt
    12481 ttggtttaat atgtccttac aaatagaaat gggtctttac acttatctaa gatttttcct
    12541 aaatcgacgc aactgtactc gtcactacac gcacatacaa cggagggggg ctgcgatttt
    12601 caataatgtg tgatgcagat cacacaaaac actcaattac ttaacataaa tgtataagtc
    12661 atacttttgt tttgggtgta tttcaatatg ttaaaaagtt tttcgctacg ctagcaagca
    12721 aaaatgaaac aggaataatc gaaatgggat gttgcgcaca gttaaaataa atcaccgtaa
    12781 ataatcatct gctataaata atcactttca tgcaatacca gataagctat ttttaaacag
    12841 acacttaccg cacaacaaac taattaaata caaagaaagg caacaatcaa cttaatgtta
    12901 atgaaatgtt ttacatatat taaccacttg tttaatgcgg agaatcagta cactattttc
    12961 ttataattac atttgaaatt atatgatcac cgggagtgat tgattaattg tctttactaa
    13021 taatcagact aatatttacc tgtttgaccg agttgggatt gcgtcgtttc tccattagga
    13081 gtaaagcttt aatgtcacct gaagttcaca gaataaggaa caggttatga gctatagcaa
    13141 tattcttgtc gctgttgcgg ttacaccgga aagtcagcaa ctgctggcaa aagcggtatc
    13201 tatcgccagg ccagtaaagg gacacatcag tttaattact ctcgcttccg acccggaaat
    13261 gtacaatcaa ttagccgcgc cgatgcttga agatttacgc agcgtgatgc aagaagaaac
    13321 gcaaagcttt cttgataagt taattcagga tgcggggtat cccgttgaca agacgtttat
    13381 tgcctacggt gagttaagcg aacatattct ggaagtatgt cacaaacatc atttcgattt
    13441 ggtgatttgc ggtaatcaca atcacagttt cttttcgcga gcatcctgct cggcgaaaag
    13501 agtgattgcc tcaagtgagg tcgatgtgct gttagttcca cttacgggag attaaccgct
    13561 cctacgcaag ctttggaaag gtagcaactt tatcgcgctg ctggctttcc gcgcttcgcg
    13621 gaactatctg ctttaggtcg ctaatgaagc actcctgcca gtggttaata tcgtttttca
    13681 cgataacgtc cagcatttct gcatgacggg aaatacgttc cgccagcgac atagtcaatg
    13741 cacgatccag cgcagctgca acttcgtcac gatcgtaggg gttaacaatt aacgccgacg
    13801 ttaactcgtt tgccgctccc gcaaattgcg aaagaacaag aacgcccggg ttggctgggt
    13861 cctgagcagc aacatactct tttgctacca ggttcatccc gtcacgcagt ggcgtcacta
    13921 agcccacgtc agagtagcgg aatattttca tcagtaattt acggtcaaaa tgctgattca
    13981 aataataaag cggcgtccag cctaattgcc cgtatttacc attaattcgt ccagcttcat
    14041 tttcgagctg atgacgaata tcctgatagg cttgcacatc accacgcgac gttggtgcaa
    14101 tctgggtata acgaatttta ccatgatgct gcggatattt ttccagcaac gcttcatagg
    14161 cgagaaaacg ctctggcaaa cctttggaat aatccagccg ttcgacagaa aagatatttt
    14221 gtacgttttt cagttccgct ttaagttgcg ccagttttgg cggcagtggc ccggcagcct
    14281 gtttggctat ttctttcggt tcaatgccga tcgggtagac ttctgttcga aatgctttgc
    14341 cccaggctgt atggcttttt gcgctacgtg tcgtgacgcg ggtcaggtta gaaagacaat
    14401 ccaggaacgc cagacgatcg ttttctgtct ggaaacccag caaatcataa tcacaaagct
    14461 gttcaagcaa ggtgtcatat gtcggcagcg cgttgaagat ttccggtgtc gggaaaggaa
    14521 tatgcagaaa gaaaccaatg cgattattca ctccccgttt gcgtaattca tgcgcaaatg
    14581 gcaacaggtg ataatcgtgg atccagataa tgtcatcgtc ttgcaacagc ggcagtaatt
    14641 tatctgccag caacgcattt acgcgtagat agccgtccca ggcaggacgc tgaaattgca
    14701 ccagatcgag ccgataatga aaagcgggcc agagaacggc attggagaat tggttgtagt
    14761 attcgtcaag gtcctgttcg ctgaggttaa aagaggccca cgtaatgtta ccttttttca
    14821 ccttttttag cggctgatcc tcattccctg tttcaccact ccagccaaac cacagtccgc
    14881 ctgcggcttt cagtgccccc agtatgccaa cggcaaggcc accggcactg gcggcgtgct
    14941 cgtctggtgg tgcaatccgg ttagatacta cgactaaacg actcatagtc atcactcctg
    15001 ttattttctc ttttttgttg taatgcggtg gttatcattt caagccagct ccagacatcc
    15061 ggcacacccg ccagtcgcca tgatgcctga gttgcacctg tgccaatttt tactgacatt
    15121 ccgcccagtc ggttaacgac tgcgaagcca gattcatcgg ttaaatcatc gcccagaaat
    15181 acgggcgttc gcccgataaa gggagcttcc tgcataaaag ctgcaattgc ctcaccttta
    15241 ctggtacctc tcggtttgat ctcgacaaca cactttccct gctgtaacgc catttgtggc
    15301 cagatctgag taatacgttg cgctaatgtc attaatgcgt cttcatgctg cggagcctga
    15361 cgataatgca gcgcaaaagc catccctttc gcctccagct ccgcgccggg atactgagcg
    15421 atgactgtat gcagttgcac gctaatatca cgcgcaatcg catccggcag atgaacgata
    15481 tgtgttttac cattgatgtc acggcgctcc gccccatgca cgcccgctaa cgggaagcga
    15541 taaggttttg ccagtgcgtc aagctccacc attgagcgcc ctgatatcaa tgccaatgca
    15601 ccatcacttg cggttgccag tagctgtagt ccttgcagaa tattgtcagg cacgacgacc
    15661 tgatcgggat gcggtttgat ttccgccagc gttccatcaa gatcaaaaaa ccaggcatat
    15721 ttcgcggata gttcaggggt ttcggttaac ggttctgtca cccggctctc ctccttctct
    15781 tttcttattg ttttgcgcag aacgcgcttt acagacatgt aagtatagac agtgtgacgg
    15841 gggtcgccat ttgcaatgaa gaacagccaa ccgttgtggc ggctggctgt ggtgggaaaa
    15901 aacgttaaat tgttgtggaa aaaagcatca gacagtgcgt ttcgcttttt gcttgtaacg
    15961 gtcgaagatc accgctgcca gcaggattaa gccgcgaacc acgtactgcg cgaaaggaga
    16021 aatattaagc aggttcatgg cgttttccac ggtgcctaaa attaagatac ccgccaccac
    16081 atatgagatt tttccgatgc cacctttcag agaaacgcca cctaaaacgc aggcggagat
    16141 aacaataagc tcataaccaa tcgacgtcat tggctgccca ctagtcatac gtgaagccag
    16201 aataattccg gctatcgcag ataccaggcc agagagaaca aagataataa ttttggtgcg
    16261 aacaaccggt acacccgcca gacgcgcagc ctcttcgttc ccgccaatcg ccagggtgtt
    16321 acgaccaaag gtggttttat tcagcaacaa accaaagata atcagacacg cgacggtgag
    16381 ccagattggc gcaggcagac cgaaccagtt agcgtaacca agggcaaaga agctttcatc
    16441 ttcgataccg accgctttac cgtctgaaat gatgtacgcc agacctcgaa caatctgcat
    16501 cgttgccagt gttgtgatca gagcatttat tttcagtttg gcgataacaa agccattgac
    16561 caggccacag agaatgccaa gcagcaaccc cgctgccacg ccaatccaca ggctttcagt
    16621 caggttgata accaccgccg tggtgacacc cgcacaggca attacggagg cgacagaaag
    16681 gtcaaagtca ccggaagcaa ggcagaacaa catgccacac gccaccatcc ccgacatgga
    16741 aattgccagg cccaaccctt tcatattaat gaaggtggca aaatttggga caaaaatggc
    16801 acaggcgata aagagcaccg caaacaccac cagcatgccg tactgatccc aaatacgccc
    16861 gaagctgaat gacgacttag gtgcgccaga acccgatgta gaaacagaag acatcatact
    16921 ctccttactc aggcaactgc ctggctgact ttaggcatcg caaggctcag tgcctgacgc
    16981 tcatctgcct gctcgtgtaa caattcaccg gcgatttcac cttcccgcat caccacaatc
    17041 cggtcggcaa cgccgaggac ttcaggtaag tcgctggagg caaacagcac cgccacaccc
    17101 tgcgccgcca gcgcataaat tacgttatat atttcgtgct tagcaccaac atcaatgctg
    17161 cgcgtaggtt catccagcaa aatgaccttc atctcttccg ataaccagcg gcccagaatg
    17221 gctttttgct gatttccgcc tgagagattc atgatcagtt gctcagcgcc cggcgttttg
    17281 atgttgagcg aacgaatgtg ctgatcggca ttgttttctt cccaaccgtt gttgattaca
    17341 caaccgccga gcacatgttt gcgtctggca ctgatgttga tattgtcgcg aacggagtgc
    17401 acgggaataa tgccttccgc tttgcgatct tccgggcaga gcatcatgcc tgcggcaatg
    17461 gcgtggctcg gtttacgaat atcgatcggc tgttggtcga tataaacctg accggcggtg
    17521 atttgcgtcc cgccaaacaa gcctttcatt aattcgctac gccccgcccc taccagacca
    17581 aacagcccaa caatttcacc actgcgaacc gccagactta ttggcgtacg cacgcctggt
    17641 gctttcacag catcaagacg caggcgctcc tcgccataac tacgaggttg ccagccgtag
    17701 atatcgccaa tgtcgcgccc gaccatcgcc tgcaccagcg cgtcgtggtc aacctgctgc
    17761 atatcggtaa aggttttgac ataacgtcca tctttaaaga cagtgatggc atcgctgagg
    17821 gcaaatattt cttccatacg gtgagaaacg tataagatca cccgcccctc ttttcgcagt
    17881 tcacgaataa cgcggaaaag attgtcgatt tcgcgggcag aaagggagct ggttggctca
    17941 tcaaaggcga taattttagc gttacgcgcc agcgccttgg cgatttcaac catctgccac
    18001 tgaccaatgg agagatattt cagcggcgtg tccgggtcaa tatccatacc aagatgttta
    18061 agttgtaaac ccgcctcata attcagcaat gagcgattca caatgccgcc tttatgcggc
    18121 agctggccga gatagatgtt ttccgcgacg gtcatttccg gcacgagatg cagttcctgg
    18181 taaataatcg ccacacccgc gttaagtgct gcggtcgtgt cggaaaagga catttcctgc
    18241 ccattaatca ctacagaacc cgtggttggc gcatagttgc cgctgaggat ttttaagaga
    18301 gttgattttc ctgcgccatt ttcacccatc aacgcatgaa cctgaccggc atagcagtca
    18361 aaactaatat ccgtcagcgc cttaacgccg ggaaacgttt taccgatgcc gcgaaatgag
    18421 agatacgggg tagactgttg cataacgtct ccgtgaatca ctggtcgttc acaccccctc
    18481 tgcatcatgc agaggggaat ttttccggca aattacttac cgcctaaacc ttttttctcc
    18541 agttcttctt taaagttgtc acgcgtgatc agcaccacgt cggtaacttc ggtaaatttt
    18601 ggcggttcaa cgtcttttgc tacccagttg taaagcattt cgctggattt atagccatgt
    18661 acgtccgggc ttggcagcag ggaaccgtag aagccggttg cctgtgcttt agacagttcg
    18721 ctcaacgcat ccacaccgtt aatgccaatg ccgatgatat cggcagcttt aaagccctga
    18781 ccttccgtcg cgcgtacgcc gcccagcacg gtgctgtcgt tcataccgac gatcagccaa
    18841 tgtttaactt ccggatgttg aaccagcatt gagttggcag cgtcaaatgc ccccgggatg
    18901 tcgttagatt tggtaggtac ctgataaatt tgtttttccg ggaatccggc cgctttcagc
    18961 gcatccatag aacccgttgt acggcgacgt gccgtatcca gttcgttggc ggtaatcgcc
    19021 atcaccgcgc tttctttgac atcccagcca cgtttctgca tctctttata cagttcctga
    19081 ccctgacgtt cgccaatttt agtcgccgcc atcatcacca gcggaacggt atccattggc
    19141 ttacctttgg cgttaacaaa ctggtcatcc acggcaatga ctttcatatc gtagccacgc
    19201 gctttcgcga cgatggcaga gccgagtttg gggtccggag tacaaataac gaaacctttt
    19261 gcgccactgg cagccaggct gtcgatcgcg ttcaatgttt tttcgccatc cggcacggca
    19321 atcttaataa cctcaaaccc taaatccttc ccggctttat cggcaaactt ccattcggtc
    19381 tggaaccacg gctcttccgg ttgcttcacc agaaaaccga gcttcaggtt ctccgccata
    19441 gcggattgtg acataacggc tgccagacca atggctgcca gggctttagt aaatttgtgc
    19501 atggttctct ccagctttag tgtcgttttg tgtagggcaa aaacgaatga ctttcgttaa
    19561 attaatcgga aaacaaagca ttacctttta tctaaaagat aagtgaccgt gttgacatag
    19621 ttttagcgag aaattaattc tccataggag agcaatatca catcgcagaa ttacagtgag
    19681 aacgtgcata aatttagcgg gaaaagacat aagggaaagc caatttgtca gacaaattgt
    19741 cgaatgcaca gcagattaat ccataagatt agcctggaaa tccttgttgt ctttggtacc
    19801 catgcgggat gtcttctttt taaccagtca ataggccgca ttacctggcg ttgagttttt
    19861 gaaatggtgt aataaccgca actcaaagat gtggaaaatg cacgtcattc atttcgccat
    19921 taattatcac tgtgctcatt aattaacaga acacgtataa tgagagccat ctcgcaaaaa
    19981 tgaaaaaacg ttttataaaa tcatcacttc atcatgaatt caaattcatt gattaatatc
    20041 aacaagatac aaaaagcact atcattaaaa ttcattgcag ttacattgat ttcatcaatg
    20101 aaatgtaaaa atatataaac ttgatgattt aagcattttc ttatacccgt tcagacgtta
    20161 ttcttatttc agatcatcgt cagaattgac tccacgatca catttcggac cggcagaaag
    20221 gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc tccacttctg
    20281 gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg
    20341 aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg ttctgaacag
    20401 agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt gacccaaatg
    20461 atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa agccattgat
    20521 gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat ggaagaatac
    20581 gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact gaatgatgat
    20641 tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg tctgttgctg
    20701 caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc tgtgcagacc
    20761 gaccaacatg ttctgaatgt cgtgtcacac cagctgcatt gatcatcatc ggcgctaatg
    20821 cattgcgccg atgaaggttt tgagaaaccg ctgcctcatc tgtttgaagc agcggttttt
    20881 ttaatgggat tcaccctgtg gggtaaactt gagttcaata agcgcgatgg ctttttggat
    20941 tgcccgcatg gtgaccgggt ctgcggcggc gggatggtta gtaaagtcga tattcttcag
    21001 ctgactggac attttttcac gaacttcaac gggcgcgatt acattgagaa catccagaat
    21061 ttgtttgata accaactggc aagcaaccac atcagaaacc agttcctgat cggcattcag
    21121 cggctgggac atcgtaaact cctgatagca ttttgaaagc cgttatagta gcggcttcac
    21181 atcttcagcg atagtcacat ccaccgtcat caggacacaa aaaaacctgc cggagcaggt
    21241 tttttgttat cggaacatat tgcctggcgg tacgtctttg aacgtcttgc aatagttatt
    21301 gaacatactt ttcaggattt tgcgcagttt catcgcggca ctccgaccat ttgttataca
    21361 ggtgttattg tctttgcgct tataatatga caaccatcac aaaaatcaat ctttatgtga
    21421 tacaaatcac ataaataccc ctttaatgtt ataaaaatga taatcaaaaa acagcccccc
    21481 tatttctgac acctacagat ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt
    21541 gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac tcttctggct ggaccatgag
    21601 acttctgatt ctgactcttt cattaataac gctggcgggg tgtacggtta ctcgccaagc
    21661 ccatgtgagc gaagttgacg cggcaacagg tattgtacgg ttggtttatg accaggcttt
    21721 tttgcagcat gcccataccg atcgttatgt cagtcgcggg atcgccgatc gcgcatgcca
    21781 gcaggaaggc tatacccacg cggtcccctt tggtcagcca gtaggcaact gtagcctttt
    21841 tgcgggttcc ttatgtttga atacggaatt cactttgtca tatcagtgcc atcattccgc
    21901 cttccctgtc tttctctaaa tattttaatc agcgagggga tcttcgctga ttaaagaaat
    21961 agcggtaatg cgttttattc cca
//
LOCUS       DAQSWQ010000068        21684 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.068, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000068 DAQSWQ010000000
VERSION     DAQSWQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.068"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..182)
                     /locus_tag="R8G18_002696"
     rRNA            complement(<1..182)
                     /locus_tag="R8G18_002696"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(560..1105)
                     /gene="hemG"
                     /locus_tag="R8G18_002697"
     CDS             complement(560..1105)
                     /gene="hemG"
                     /locus_tag="R8G18_002697"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HEE9796352.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(1117..2568)
                     /gene="trkH"
                     /locus_tag="R8G18_002698"
     CDS             complement(1117..2568)
                     /gene="trkH"
                     /locus_tag="R8G18_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HEE9796353.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2607..3221)
                     /locus_tag="R8G18_002699"
     CDS             complement(2607..3221)
                     /locus_tag="R8G18_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HEE9796354.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(3221..4552)
                     /gene="pepQ"
                     /locus_tag="R8G18_002700"
     CDS             complement(3221..4552)
                     /gene="pepQ"
                     /locus_tag="R8G18_002700"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HEE9796355.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4742..6931
                     /gene="fadB"
                     /locus_tag="R8G18_002701"
     CDS             4742..6931
                     /gene="fadB"
                     /locus_tag="R8G18_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HEE9796356.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6941..8104
                     /gene="fadA"
                     /locus_tag="R8G18_002702"
     CDS             6941..8104
                     /gene="fadA"
                     /locus_tag="R8G18_002702"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HEE9796357.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8294..8995)
                     /gene="fre"
                     /locus_tag="R8G18_002703"
     CDS             complement(8294..8995)
                     /gene="fre"
                     /locus_tag="R8G18_002703"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HEE9796358.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(9042..10535)
                     /gene="ubiD"
                     /locus_tag="R8G18_002704"
     CDS             complement(9042..10535)
                     /gene="ubiD"
                     /locus_tag="R8G18_002704"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEE9796359.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10702..11190
                     /gene="rfaH"
                     /locus_tag="R8G18_002705"
     CDS             10702..11190
                     /gene="rfaH"
                     /locus_tag="R8G18_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HEE9796360.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(11187..11969)
                     /gene="tatD"
                     /locus_tag="R8G18_002706"
     CDS             complement(11187..11969)
                     /gene="tatD"
                     /locus_tag="R8G18_002706"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HEE9796361.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(12011..12787)
                     /gene="tatC"
                     /locus_tag="R8G18_002707"
     CDS             complement(12011..12787)
                     /gene="tatC"
                     /locus_tag="R8G18_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HEE9796362.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12790..13305)
                     /gene="tatB"
                     /locus_tag="R8G18_002708"
     CDS             complement(12790..13305)
                     /gene="tatB"
                     /locus_tag="R8G18_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HEE9796363.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13309..13578)
                     /gene="tatA"
                     /locus_tag="R8G18_002709"
     CDS             complement(13309..13578)
                     /gene="tatA"
                     /locus_tag="R8G18_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEE9796364.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13657..15297)
                     /gene="ubiB"
                     /locus_tag="R8G18_002710"
     CDS             complement(13657..15297)
                     /gene="ubiB"
                     /locus_tag="R8G18_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEE9796365.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15294..15899)
                     /gene="ubiJ"
                     /locus_tag="R8G18_002711"
     CDS             complement(15294..15899)
                     /gene="ubiJ"
                     /locus_tag="R8G18_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HEE9796366.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15913..16668)
                     /gene="ubiE"
                     /locus_tag="R8G18_002712"
     CDS             complement(15913..16668)
                     /gene="ubiE"
                     /locus_tag="R8G18_002712"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEE9796367.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16763..18190)
                     /gene="rmuC"
                     /locus_tag="R8G18_002713"
     CDS             complement(16763..18190)
                     /gene="rmuC"
                     /locus_tag="R8G18_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEE9796368.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18331..19092)
                     /gene="udp"
                     /locus_tag="R8G18_002714"
     CDS             complement(18331..19092)
                     /gene="udp"
                     /locus_tag="R8G18_002714"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEE9796369.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19354..20166
                     /locus_tag="R8G18_002715"
     CDS             19354..20166
                     /locus_tag="R8G18_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEE9796370.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20560..21621
                     /gene="hcp"
                     /locus_tag="R8G18_002716"
                     /pseudo
     CDS             20560..21621
                     /gene="hcp"
                     /locus_tag="R8G18_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5319 a         5805 c         5418 g         5142 t
ORIGIN      
        1 ttatgcggta ttagctaccg tttccagtag ttatccccct ccatcaggca gtttcccaga
       61 cattactcac ccgtccgcca ctcgtcagcg aagcagcaag ctgcttcctg ttaccgttcg
      121 acttgcatgt gttaggcctg ccgccagcgt tcaatctgag ccatgatcaa actcttcaat
      181 ttaaaagttt gatgctcaaa gaattaaact tcgtaatgaa ttacgtgtcc actcttgaga
      241 cttggtattc atttttcgtc ttgcgacgtt aagaatccgt atcttcgagt gcccacacag
      301 attgtctgat aaattgttaa agagcagtgc cgcttcactt tttctcagcg gcgcggggtg
      361 tgcataatac gctttcccgc tacagagtca agcatttatt tttgctttct ctgtcggaat
      421 tctcaggaga accccgctga cccggcggcg tgtttgccgt tgttccgtgt cagtggtggc
      481 gcattatagg gagttattcc ggcctgacaa gaggaaattt aaaataattt tctgaccgcg
      541 cattttttat tctttatgct tatttcagcg tcggtttgtt ggttaactgg gcgatttctc
      601 gggcgaaatt cgccacctgc tcccaatcgg tatagacaac ttctttgcgc gtatccgttt
      661 caccgcctga catcttcata attagcttga tcataaaacg atcgtaccag cgataacgtg
      721 ggtaacgcag cgccccggca atgaccgcgc agcgatcggg acgccattgc gagttcatca
      781 gaaacttccg cgcgtagctg ttggtctgtg gagtacgctt ctccggtttg cgcgccacca
      841 ggtttacgga ataaaaggcg ctcggcatcg aattcagtcg cgtcgcatgt tttttgacaa
      901 attcctggaa cgcagagtgg tagtgaccat agcgaataga agcaccaatg accacacggt
      961 cataattttc ccattgtggt tcttcaatgc ggtgcacatt ggcgacatcc gtctggatcc
     1021 ccagttcttt cagttccgaa gccaggtagg aggcaatctc gcgcgtttgt ccgtcccttg
     1081 ttgagaaaag aattaatgtt ttcacgtatt actccatcat tcacgccaga aagtcggggt
     1141 aaagagcacc agcaatgtaa agacctcgag acgaccaaac agcatgttgg caatcaggat
     1201 ccatttagcc accgggttca tactggtaaa gttatcagca accacgccaa gccctggccc
     1261 caggttattc aatgtcgcaa caactgaagc aaaggcagaa aagtcatcca cgcccgtggc
     1321 gataatcgcc agcatactga caataaacac caatgcatag gcggagaaaa atccccaaac
     1381 ggcttcgagg atacgttccg gcagtgcgcg attccccagc ttaatgctat agacggcgtt
     1441 cggatgcacc agtcgtttca gctcacggtt cccctgctta aacagcagca ggatgcggat
     1501 cactttcagg ccaccgcccg ttgacccggc gcaaccgccg ataaaagctg aacataaaag
     1561 cagtaccggc aaaaagagcg gccagcgggc aatgctgtca gtcgtaaacc cggcggttgt
     1621 cgccatcgat accacctgga aaaacgcctg gttaattgtc atcagcgccg aactgtagac
     1681 attatgaaac cacagtacga gtgtacaaat aaccaccagc gtaaactgta cgccgataaa
     1741 catgcgaaat tccggatcgc gccaataaac cttcagacta cgcccactta acagtgaaaa
     1801 gtgcagaccg tagttacagc cggagatcag caggaagata gcaatgatag tgttaatagt
     1861 cgggctgtcg aaataaccga tactggcatc atgtgtcgag aagccgccaa tagcgatagt
     1921 cgcaaagcta tggccgatgg catcaaaggc atccattccg gcaaaccaca acgccagcgc
     1981 acaggcgacg gtcagcaaga cataaatcaa ccacagggtt ttcgccgttt ccgcaattcg
     2041 cgggcgcatt ttgttatctt tcagcgggcc gggcatttct gcgcgataga gctgcatccc
     2101 acccacgccg aggataggca gtatcgcaac cgctaacacg atgatcccca tcccgccaaa
     2161 ccattgcagc atctggcgat aaaagaggat ggcatgaggg agcgaatcca gccccaccag
     2221 cgtagtggcc ccggtggtgg tcaggccaga gaaagattca aaaaacgcat cggtaatcgt
     2281 gaggttcggg ctttccgaga agataaaagg gagcgcaccg acactaccca gcacggtcca
     2341 gaacagcacc actatcagaa acccctcacg ggatttcagt tcgcccttct ctttacggtt
     2401 cggccaccac agcatagaac caatggcgag ggcgacaaaa aaggtctggg taaaagcgcg
     2461 gcccgctcca tcccggtaga tgagtgctac cagcccaggg ataatcatgg tccctgaaaa
     2521 taagatgacc agtagtccaa cgattcgggt aatggcgcga aaatgcatct ctgccgcttc
     2581 cttagttctg caaaacgaag tggggattat tcttcaatcg ctaacaattg caatgaacca
     2641 cggctaaaat ccgccagctt tgcggaaaat tcagccactt tcgccgccgg aagcgccacc
     2701 cgcagcagaa cgaatgcctg ataatcactg ttgataattt tgccgtcaca ctgccccagc
     2761 aacgcttcaa tgccggttaa ctgatgatat tcacattgca aagtatattc ggttaatggc
     2821 gtcttgcgtt gggtcgttag ctggcgcagc gcctgattca cgccgccgcc atacgctttc
     2881 actaacccac cggtgccaag caatatgccg ccgtagtagc gcactaccac agcggtaatt
     2941 tccccgacgc cgctgcccat tagctgggcg agcatcggtt tacctgccgt tcctgccggc
     3001 tccccgtcgt cagagaaacc cagctgttga gaatcatccg gcgcacccgc cacccacgcc
     3061 acgcaatggt ggcgggcatc ggggtgttct gcccgcaccg attcaacaaa cgctttcgcc
     3121 gcctcaacgc catcggtatg cgccaacatc gttatgaagc ggcttttttt gatctcttca
     3181 acgaccgtga ccggtgccgc aggaattaac cagctttcca tcacgccagt ttcagatccc
     3241 gggtcatgtt ttccacgttg ttttcgtgga tcaccacgtt gtcttcgata cgaatgccgc
     3301 cgaacggttt cagtgcttca attttctgcc agttgaagtg cttgctgaac tgcccttcac
     3361 gccacggtgc cagtagcgat tcaatgaagt agatacccgg ttcgatggtt aacaccatac
     3421 ccggctggag aatacgggtg cagcgcaggt acggatattt tgccggtgcc gcgaggtgtg
     3481 taccgctatc atcctgcata aaaccggcta cgtcatgcac ctgcaggccc agcggatgac
     3541 cgataccatg cggcataaac ggtccggtga gatcgttttc gaccatcgct tcttcactca
     3601 tatcggtgat gatttgatgt ttacgcagca gtttggcgat gcgctgatgg aactggatgt
     3661 ggtaatccac atagctaacg cctgctttca tggtggcgat cagcgccagt tgttcatcat
     3721 ttacgtcttt caccagctgt gcgtagtcgt tgtcgctttt tgccgaccag gtacgagtca
     3781 ggtcagccgc atagccgtta tattcggccc cggcatccag caggaagctg cgcatctctt
     3841 ccggtgcctg atgatccagt ttggtgtaat gcagcaccga agcgtgttcg ttaagcgcca
     3901 caatgttgct gtaaggtacg tcggtatcac gatgaccggt cgcggtcaga taggcgatgt
     3961 tgatatcgaa ctcgctcatg ccagaacgga acgcttcttc tgccgcacga tgaccgttga
     4021 ccgccatttt ctgcgcttca cgcatacagg ccagttcgta ctcggttttg aaggagcggt
     4081 agtaatgcag gtagtcgatc acccctttcg ggttgatgtt gctggcctca atacccagtt
     4141 gcagcgcacg ttccggcacc ggaccgatat aaccgatatt gccgcgcgca gcgggcagca
     4201 ggctaccaat gccatcggct ttcggcagtg cgatcacttc cacatcttca gtccagaagg
     4261 aggtcggcag cggttcgacg ttgtgccagt aatcaaccgg cagatagaac cacagtttcg
     4321 gcttgttcac gccatccacc agcaaccagc agtttggcac ctgagttacc ggcacccacg
     4381 ctttgaattg cgggttcact ttaaacggat agggatgatc gtcgagaaaa acgttgaaca
     4441 gctcgccgga gtgaataagt aatgcatcca gcttgaagcg cgccagcgca tcgcgagtcc
     4501 gttcttgtaa ggtagctata tgatttttat agagcgaggc cagtgattcc attttttacc
     4561 cttctgtttt tttgacctta agtctccgca tcttagcaca tcgttcatcc agagcgtgat
     4621 ttctgccgag cgtgatcaga tcggcatttc tttaatcttt tatttgcata tttttaacac
     4681 aaaatacaca cttcgatgca tctggtacga ccagatcacc ttgcggattc aggagactga
     4741 catgctttac aaaggcgaca ccctgtacct tgactggctg gaagatggca ttgccgaact
     4801 ggtatttgat gccccaggtt cagttaataa actcgacact gcgaccgtcg ccagcctcgg
     4861 cgaggccatc ggcgtgctgg aacagcaatc agatctaaaa gggctgctgc tgcgttcgaa
     4921 caaagcagcc tttatcgtcg gtgctgatat caccgaattt ttgtccctgt tcctcgttcc
     4981 tgaagaacag ttaagtcagt ggctgcactt tgccaatagc gtgtttaatc gcctggaaga
     5041 tctgccggtg ccgaccattg ctgccgtcaa cggctatgcg ctgggtggtg gctgcgaatg
     5101 cgtgctggcg accgattatc gtctggcgac gccggatctg cgcatcggtc tgccggaaac
     5161 caaactgggc atcatgccgg gctttggcgg ttctgtacgt atgccacgta tgctgggcgc
     5221 tgacagtgcg ctggaaatca ttgccgccgg taaagatgtc ggcgcggatc aggcgctgaa
     5281 aatcggtctg gtggatgggg tagtcaaagc agaaaaactg gttgaaggcg caaaggcggt
     5341 tttacgccag gccattaacg gcgacctcga ctggaaagcg aaacgccagc cgaagctgga
     5401 accgctaaaa ctgagcaaga ttgaagccac catgagcttc accatcgcta aaggaatggt
     5461 cgcgcaaacg gcggggaaac attatccggc ccccatcacc gcagtaaaaa ccattgaagc
     5521 tgcggcccgt tttggtcgtg aagaagcctt aaatctggaa aacaaaagtt ttgtcccgct
     5581 ggcgcatacc aacgaagctc gcgcactggt cggcattttc cttaacgatc aatatgtaaa
     5641 aggcaaagcg aagaaactca ccaaagacgt tgaaaccccg aaacaggctg ctgtgctggg
     5701 cgcaggcatc atgggtggcg gtatcgctta ccagtccgcg tggaaaggcg tgccggttgt
     5761 catgaaagat atcaacgaca agtcgttaac cctcagcatg accgaagccg cgaaactgct
     5821 gaacaagcag cttgagcgcg gcaagatcga tggtctgaaa ctggctggcg tgatctccac
     5881 gatccaccca acgctcgact acgccggatt tgaccgcgtg gatattgtgg tagaagcggt
     5941 tgttgaaaac ccgaaagtga aaaaagccgt actggcagaa accgaacaaa aagtacgcca
     6001 ggataccgtg ctggcgtcta acacttcaac cattcctatc agcgaactgg ccaacgcgct
     6061 ggaacgcccg gaaaacttct gcgggatgca cttctttaac ccggtccacc gaatgccgtt
     6121 ggtagaaatt attcgcggcg agaaaagctc cgacgaaacc atcgcgaaag ttgtcgcctg
     6181 ggcgagcaag atgggcaaga cgccgattgt ggttaacgac tgccccggct tctttgttaa
     6241 ccgcgtgctg ttcccgtatt tcgccggttt cagccagctg ctgcgcgacg gcgcggattt
     6301 ccgcaagatc gacaaagtga tggaaaaaca gtttggctgg ccgatgggcc cggcatatct
     6361 gctggacgtt gtgggcattg ataccgcgca tcacgctcag gctgtcatgg cagcaggctt
     6421 cccgcagcgg atgcaaaaag attaccgcga tgccatcgac gcgctgtttg atgccaaccg
     6481 ctttggtcag aagaacggcc tcggtttctg gcgttataaa gaagacagca aaggtaagcc
     6541 gaagaaagaa gaagacgccg ccgttgaaga cctgctggca gaagtgagcc agccgaagcg
     6601 cgatttcagc gaagaagaga ttatcgcccg catgatgatc ccgatggtca acgaagtggt
     6661 gcgctgtctg gaagagggca ttatcgccac tccggcggaa gcggatatgg cgctggtcta
     6721 cggcctgggc ttccctccgt tccacggcgg cgcgttccgc tggctggaca ccctcggtag
     6781 cgcaaaatac ctcgatatgg cacagcaata tcagcacctc ggcccgctgt atgaagtgcc
     6841 ggaaggtctg cgtaataaag cgcgtcataa cgaaccgtac tatcctccgg ttgagccagc
     6901 ccgtccggtt ggcgacctga aaacggctta aggagtcaca atggaacagg ttgtcattgt
     6961 cgatgcaatt cgcaccccga tgggccgttc gaagggcggt gcttttcgta acgtgcgtgc
     7021 agaagatctc tccgctcatt taatgcgtag cctgctggcg cgtaacccgg cgctggaagc
     7081 ggcggccctc gacgatattt actggggttg tgtgcagcag acgctggagc agggttttaa
     7141 tatcgcccgt aacgcggcgc tgctggcaga agtgccgcac tctgtcccgg cggttaccgt
     7201 caatcgcttg tgtggttcat ccatgcaggc actgcatgac gcagcacgaa tgatcatgac
     7261 tggcgatgcg caggcatgtc tggttggcgg cgtggagcat atgggccatg tgccgatgag
     7321 tcacggcgtc gattttcacc ccggcctgag ccgcaatgtc gccaaagcgg cgggcatgat
     7381 gggcttaacg gcagaaatgc tggcgcgtat gcacggtatc agccgtgaaa tgcaggatgc
     7441 ctttgccgcg cggtcacacg cccgcgcctg ggccgccacg cagtcgggcg catttaaaaa
     7501 tgaaatcatc ccgaccggtg gtcacgatgc cgacggcgtc ctgaagcagt ttaattacga
     7561 cgaagtgatt cgcccggaaa ccaccgtgga agccctcgcc acgctgcgtc cggcgtttga
     7621 tccagtaagc ggtacggtaa cggcgggtac atcttctgca ctttctgatg gtgccgcggc
     7681 catgctggtg atgagtgaaa gccgcgcccg tgaattaggt cttaagccgc gcgcccgtgt
     7741 gcgttcgatg gcggtcgttg gttgtgatcc gtcgattatg ggttacggcc cggttcctgc
     7801 ctcgaagctg gcgctgaaaa aagcggggct ttctgccagc gatatcggcg tgtttgagat
     7861 gaacgaagcc tttgccgcgc agatcctgcc gtgcattaaa gatctcgggc taatggagca
     7921 gattgacgag aagatcaacc tcaatggtgg tgcgatcgcg ctgggtcatc cgctgggttg
     7981 ttccggtgcg cgtatcagca ccacgttgct gaatctgatg gaacacaaag acgttcagtt
     8041 tggtctggcg acgatgtgta tcggtctggg tcagggtatt gcgacggtgt ttgagcgggt
     8101 ttaaccactt gccggatgcg gcgtgaacgc cttatccggt ctaccgatcc tgcaccaatg
     8161 taggcctgat aagacgcgcc agcgtcgcat caggcatcgt gcaccgattg ccggatgcga
     8221 catagatcta aatgccactg aataaagttt tagttgccgt tcttcccgcc tgtcaggggc
     8281 gggtttttat atctcagata aatgcaaacg catcgccaaa cagacgatct tcccgcgcat
     8341 tacgctcact gcaaaacagg tcacgagcaa ttttcgccat ctcaaaacgt ccggcaatat
     8401 agatatcatg ctctgccagc gtaccatgat cctgcaagac cgccgttaac acggtgccag
     8461 tacgcccacg ccagcccgct tccggttgtt caaccaccgg caccacttgc agaccaggat
     8521 gcttcagcga aagcgcctca agctcgcaga gatcatacag atgttgctct tcacgcccac
     8581 cccagtaaat ggtgatatcg cgattgggat tacgcgccaa cgccgtcaat aaaatcgagc
     8641 gcgcatagga gaacccggtg ccgcccgcaa tcaaaatcat cggacgctct tcatcatcgc
     8701 gcagccacgc ttctccgtgg ggaatatcga ccacgatttg atgatctttg aggatgcggt
     8761 ccatgactgc tttcgcgtaa aggttgattt cagaagcgcc aatatgcagc tcgataaacc
     8821 ctttttcatc cggcgtcgaa gccattgaaa acggacgttt gtcgcgctca tccatcacta
     8881 ccatcaaata ctgaccagca cgaaaagaaa aggccgcgtc tggtacgatt cggacacggt
     8941 atacagtatc cgtgatagct tctaccgagg tcactttaca gcttaaggtt gtcatgcgct
     9001 ttctctgtcg gatcaaataa atagggcaaa actaacgcgc atcaggcgct tttaccgttg
     9061 ttaaaaatag ccagttcatc ccagatggcg tcaatatgcg cgacgacatc aggatctttt
     9121 ttgatgggac gtccccattc acgctgggtt tcccccggcc atttattcgt ggcatccagc
     9181 cccatttttg aacccagccc ggagacaggc gaggcaaaat ccagataatc aataggcgta
     9241 ttttctacca gaacagtatc ccgcgccggg tccatacggg tggtaatcgc ccaaatcaca
     9301 tcgttccagt cgcgtgcgtt gacgtcatca tcgcaaacga tcacaaattt agtgtacata
     9361 aactggcgta agaacgacca gacgcccatc atgacgcgct tcgcgtgtcc ggcgtactgt
     9421 tttttgattg tcactaccgc cagacgataa gaacagcctt ccggtggcag gtaaaaatcg
     9481 acaatttccg ggaactgttt ttgcagaatc ggcacaaaca cttcgttcag tgcgacaccc
     9541 agcaccgcgg gttcatccgg cggacgacca gtataggtgg agtggtaaat cgcatcttca
     9601 cgctgggtaa tatgcgtcac ggtaaatacc gggaaactat cgacttcgtt atagtaaccg
     9661 gtgtggtcgc catacggccc ttccggcgca gtttcgcctt gttcgatata cccttccagc
     9721 acaatctccg cactggcggg cacttcaagg tcattggaga tacacttcac cacttcggtc
     9781 ttggtaccgc gcagcaatcc ggcaaacgca tactctgaaa gtgtatccgg aacgggggtg
     9841 actgcaccga gaatcgtggc gggatcggca cccagcgcca cagaaaccgg gaaacgttcg
     9901 cccggatgcg ccgcacacca ctcctgataa tccagcgcgc cgccgcgatg cgacagccag
     9961 cgcataatca gtttgttttt accaatcagc tgctggcgat aaatgcccag attctgccgc
    10021 tctttatgcg ggccgcgcgt tacggtcagc ccccaggtaa tcagcggcgc ggcatcttcc
    10081 ggccagcagg tcataatggg aatgcgattg agatcgacgt catcgccaga gacgattttt
    10141 tgttggcagg gcgcaccacg cagccgcttt gtcggcatgt tcaatacttg cttaaactgc
    10201 ggcagtttat caaacaggtc gcggaaacct tttggcggct cgggctcttt cagaaacgcc
    10261 aataatttac caacttcacg cagcgccgaa acatcttcct gccccatgcc catcgccacg
    10321 cgctttggcg taccgaacag gttgcacagc accggcattg agtagccttt agggttttcg
    10381 aacaacagcg caggcccacc ggcacgcaaa gtgcggtcag caatttcagt gatttccaga
    10441 tgcggatcca ccgggagcgt gatacgtttt agctcaccct gctgttcaag cagcgtcaag
    10501 aagtcgcgta aatcgttata tttcatggcg tccattgtag cctcttaatc tacgcccatt
    10561 atacggcgtt catctttgcg atgctgtaaa tttgttaaat tagcgtgaac tctgacggta
    10621 taacgcaaac cggggaatat aattaactta gcgtaaagct tttgctatcc ttgcgccccg
    10681 attaaacgga taagagtcat tatgcaatct tggtatttac tgtactgcaa gcgcgggcaa
    10741 cttcaacgtg cccaggaaca ccttgagaga caggctgtga attgcctggc accgatgatc
    10801 accctggaaa aaatcgtgcg tggaaaacgt actgcagtca gtgagccatt gtttcccaac
    10861 tacctgtttg tcgaatttga tccagaagtg attcatacca cgactatcaa cgcgacccgc
    10921 ggtgtcagcc acttcgtgcg ctttggcgcg tcgctagcga tagtcccatc ggcggtgatt
    10981 catcagctat cggtatataa accgaaagac attgtcgatc cggcaacccc ttatccggga
    11041 gataaggtga ttattaccga aggcgcgttc gaaggctttc aggccatttt caccgaaccc
    11101 gatggtgagg ctcgctccat gctattgctt aatcttatta ataaagagat taagcacagt
    11161 gtgaagaaca ccgagttccg caaactctaa aacgcaatcc caaaccgtgt tttgacatta
    11221 gcatccgtgg tggcagccag ccatgcggca tcttctccac gccagtgcgc aatacgttgc
    11281 aaaatatggg gcagatgggc tggctcgttg cgccgggatg atggctttgg cgtgagatcg
    11341 cgagggagca gatacggcgc atcagtttcg atcagtaatt tttccgccgg aatcaacggc
    11401 aaaagttccc gcagctccag tccgcgtcgt tcatcgtaaa cccaaccggt aatgccgata
    11461 taaattccat gcgccacgca cgcctgcatc tcttcgcgtg tgccggtaaa gcaatgaaga
    11521 accgcaccag gcagtttatc cagccacggc tccagcaatg tcataaaccg ctcgtgggca
    11581 tcgcgacagt gcataaatac cggcatgttt aaatctgcgg caatgcgtag ctgggcaaca
    11641 aaagcgcgtt cctgctcttc cggcgtcgaa aagttgcggt taaagtcgag accacattca
    11701 ccaatcgcca ccacttctgg ctgcgcggcc agctcaataa tcgcttcttc agtcgcagct
    11761 tgccactggc tgctgtcgtg aggatgtacg cccgccgttg accaacagga cgaatactga
    11821 cgcgccagct tttgcgcctg ctggctttca cgcaggttag tgccggtgat gagtagccca
    11881 ttaactcccg cgtcaaaagc gcgcgctaca acatcatcac ggtctttcgc aaattgcgaa
    11941 ctggtcaaat taacgccgat atcaaacatc ctgtactcca tatgacaacc gccctgacgg
    12001 gcggttgaat ttattcttca gttttttcgc tttctgcttc agcgtcgttt tcctcttccc
    12061 gatttcgccc tttaccaacg taaaagcgtg agaagaagac accgatttca aacagacagt
    12121 acatcgggat cgccaatagc gtttgcgaga agacatccgg cggcgtcagc aacatcccga
    12181 caacgaatgc accaaccagc acatacgggc gttttttgcg taagtcttct ggcgaggtaa
    12241 tccccatcca gcacagcagc acaattgcca ccggcacttc aaaggagaca ccaaacgcca
    12301 taaacagcgc cataacgaag cttaaatagc tggcgatgtc ggtggatacc tgcacccctt
    12361 ccggcgcggt attggcaagg aagccaaatg ccagcggaaa gaccacaaag taggcgaatg
    12421 ccatgccgat ataaaacagc agagagctgg aaaccagcag cggcaccacc aggcgacgtt
    12481 catgcttata cagcgctggg gcgataaatg cccacacctg atagagaatc accggcgctg
    12541 acagaatcag cgacaccata aaggtcagct tgatcggcgt aaagaacggc gaggccacgt
    12601 cggtggcgat catcgttgaa ccttgcggca actgcttgat caatggcgcg gataccaggt
    12661 gatagatgtc attggcgaaa tagaccagac acaggaatat cacgatcacc gcgataatgc
    12721 agttcagcag acgcttacgc agctcaatta gatgcgtgat aagcggttga gtatcttcta
    12781 cagacatgtt tacggtttat cactcgacga aggggaaggt gcagcggttt tcggttcagc
    12841 gtccgcagca ggttttacca ccggctctgg cgtggtttct ggcttctgtt ccggcgaact
    12901 ggcctgcgtt tgtgcagcgg caggcgttac gccctcatgc gcagcttcat tatctttcac
    12961 caccgggtta tggatggtgt gcgcttcatc gctcgccttt tcaggatcgt ttgcaacgta
    13021 ggaacgcttc atcgactccg cggcctggcg tagttcatcc atcgacgctt tcagttcggg
    13081 cgtcaggtta gtgaggctcg ccttttcaac ctttttcaga ctgtcctgaa actcctggag
    13141 ttttaactcc tgggtcagtt cgttctgcac cgttgtcgcc agtgaacgca acgcgcgaat
    13201 ccagcccgct accgttttta ccgccacagg cagtcgttgc ggccccagaa cgacgaggcc
    13261 gatgatgaac accaatagca gttcgctaaa accgatatca aacacggatt acacctgctc
    13321 tttatcgtgg cgcttcgcgt cttctgtttt agcctgttcc tgattcgtat ccgcctgctt
    13381 atcggcgata gttttcgcag taaaatcagc atcctgactg gttttatcct gctttggttc
    13441 atcatcgctc attgcttttt taaagccttt gatcgacgca ccaagatcgg aaccgatgga
    13501 gccgagcttt ttggtgccaa aaagcagtac aacgatgacg gcaataatca ataactgcca
    13561 aatactgata ccacccatac atgttcctct gtggtagatg atgattaaac aaggccgcat
    13621 tatacgttac acgacccgcc ttgagcgatg aaaaaatcag cgtgttttgc gccaaccgac
    13681 aaaccaggcg atcagaccac ctgccattaa ccagccgggc atcagccccc attcaggtcg
    13741 gctgaccaac aagaatgtgc cacttaatac taacgtagcg ccaattccga gaaaataacg
    13801 cgattgtccc tgacgtacat gatttgactg aagctcgcgg gcaatcttat caacactgtg
    13861 ctgtaaatac ttgccctggc gcaaactgtc gtaaaccaat tcaggcagtt ctggcatttt
    13921 ttcgacccag aacggcgctt tttctttaaa tgctctcacc agggcaggaa taccgacctg
    13981 atctttaatc cacgactcca ggaaaggctt cgctgttttc cataaatcga gctgcggata
    14041 aagctggcgt cctacccctt cgacatagag cagggttttc tggagtaaca ccagttgcgg
    14101 ctgcacttcc atattgaagc gacgcgccgt attaaacaga tttaacagta catgtccaaa
    14161 cgaaatttcg gccagcggtt tctcaaagat aggttcacag accgtacgaa tggcaaattc
    14221 gaactcttca acgttggtat ctggtggcac ccagccagaa tcgacgtgta gctctgccac
    14281 tttgcgatag tcgcgattaa agaaggcgat aaagttttct gccagatagc gtttatcttc
    14341 tttgtttagc gagccaacaa tcccgcaatc aatgccgata tatttcgggt tttccgggtg
    14401 ttcatagctt acgaaaatgt tgccagggtg catatcggca tggaaaaagc tgtcgcgaaa
    14461 gacctgagtg aagaacacct gcacgccacg ttccgccagc aatttcatgt tggtgccatt
    14521 tttctccagc gtcgcaacat cagacaccgg aatgccgtaa atgcgctcca tcaccatcat
    14581 cccttcacta caatagtcag ggtaaacttc cgggatgtag agcatcgggc tgtcttcaaa
    14641 atttcgccga agctgaatgg cgttggcaga ttcccgcagc aaattcagtt catcaatcaa
    14701 ggtcttttcg tactcgcgca ccacttcggt tgggcgcaga cggcgaccat ccggcagcaa
    14761 acgcggcacc cagcgagcca gacggtagat aagtttcaga tccgctttaa taaccggcaa
    14821 aatatccggg cggatgactt taatcaccac ctctttacca ttcgatttca atcgcgcggt
    14881 atgaacctgg gcgatagaag cagaagccag tggcttgatt tcaaaatcgt caaaccacgc
    14941 ttctaccggc aagccgccca ttgcagcttc aatctgctgc ttcgccagct tgccatcaaa
    15001 cggagcaact ttgtcctgca ataacgccag ctgatcggca atatgcggtg gaaaaagatc
    15061 gcggcgggtt gataacattt gcccgaactt gatccaaacc ggccccagtt cttgcagggc
    15121 cagtcgtagt cgctcaccta aaagtttgtc tttatgccga tttggcatcc agaataatga
    15181 gtatcgccat agccgtagcg gcagggtgat acgcattttg gggatcagtt catcaagtcc
    15241 gtagcttaaa aaagtgcgaa tgatgaaata taggcgccgt acttcacctg gcgtcattta
    15301 gcctccagtt tttccagccg tttggtcagg gcatcaacag cacgctcgac ggcagccgtc
    15361 tcttccgcaa accaggccac ttcaagcgga ccgggtgcca tacgccactc ttcagtaatg
    15421 gcttccgcca cataacgttg ctggcgctta atgccgtgat gcaggaactt tgcgcctccg
    15481 cgcatggctt tgctgattcc ttcagcggcg atatcaccgg tataaggggc cagcagttcc
    15541 gcagggtcga actctgccag atctgccagc gcaacgaagt tttgcaccac ctgaatatcg
    15601 ccctgcactt ccagctcacc actgcgaatc agtgcggtaa gctgctggcg atcgcgaagt
    15661 ttcggcaaca cactggcgta ggcgataacg gtgcagtcag catcgcctgc ccattcgccc
    15721 agtacatcaa cctggcgttc gctgaacacc agaatcaatg acgtcgaaaa gccttttacc
    15781 tccacgcgca atactttacc cagcagacgc gagcgggctg ttttcagcgc gggtgagcga
    15841 tacaggaagg tgttgagcag actttcaatt cctgccgtca ctaaaggttt aaaaggcatt
    15901 tccggtctcc tgtcagaact tataaccacg atgcagcgcc acaacccctg ccgtcagatt
    15961 gtagtagtcg acactttcga atccggcatc ctgcatcatg gctttcaggg tatcctgatc
    16021 gggatgcata cggatggatt ctgccagata acggtagctg tcggcgtcgt tcgcgaccag
    16081 tgagccaata cgcggcagca catggaagga gtatgcatca taggctttgc tcagcggctc
    16141 gataattggc ttcgagaact caagcaccag caggcggccg ccgggtttca gcacgcgata
    16201 cattgaacgc agtgctttat ctttgtcggt gacgttacgc agaccaaacg aaatggtgat
    16261 gcaatcaaag gtgttatccg ggaacggcag cgcctcagcg ttcgcctgaa catactcaac
    16321 gttgccaatc acaccgatat tacgcagctt ctcgcgtccc attttgagca tggattcatt
    16381 gatatcagca aggaccactt tgccagtttc tccgaccagg cgggagaatt tcgctgtcag
    16441 gtcgccggtg ccaccagcca gatccagcac ggtctgccca cggcgtacgc cgctgcaatc
    16501 aatcgtgaat cgcttccaca aacgatgaat accaaatgac atcaaatcat tcatgacatc
    16561 gtattttgat gccacggaat ggaaaacgtg ggcgaccata tccgcttttt gttccttcgc
    16621 gacggtctga aaaccaaagt gcgtcgtttc ttgtgactta tccaccatct caatgcctgc
    16681 tcatcaaaaa attgttccag aagtgtaaca gattgggcgt cgatgcccta gatttctacc
    16741 cggcttaact acccctaatg ggctagcgcg actgctgatt atattcatca tcgcgttgat
    16801 aagcttcatc attcggctgc tccggaaccg accgaagtcg atactcttca ttctggctca
    16861 ccgcctgttc agccaaatcc ggattaatct cgcgtttaat ttctactcct aaaccgcgaa
    16921 acgcttctgc ctgcgccagc acatttccgc gacctgaaga gagttttttc attgcctggc
    16981 ggtagttatc ctgcgctttg tcgagacttt gaccaatcgc ggacatgtca tcgatgaaca
    17041 aacgcatctt gtcgtacagc ttgctggcac gatcggcgat ttgctgggcg ttgcggcttt
    17101 gatgctcata acgccacagg ttggcaatag tacgcaacgc caccagcagc gtagtcgggc
    17161 taaccagcat gatattgttt ttcaacgctt cggtgatcag ctccggctgg cggtcaagcg
    17221 ccagtaaaaa agcgggttca acgggaataa acatcagcac gtaatccaga gttcgcagcc
    17281 ccggcagctg ttgataatct ttgcgtccca gcaaacggat atggttacgc accgacgcga
    17341 tatgttcctg tagcgcgctt tcgcgggtgt agtcgtcttc ggcgttaaaa tagcgttcat
    17401 aggcgaccag cgtcattttg gcgtcgatca ccacatcttt tccctgcggc agccgcacga
    17461 tgacatccgg ctgcatccgc gagcgggcgt cattttcgat gctgacctgg gtttcatatt
    17521 catacccttc acgcagaccg gaagcctcca gcacccgcgt caatactacc tcgccccagt
    17581 tgccctgggt tttattgtcg cctttcagcg cgcgcgtcag gttgatcgct tcctgggcca
    17641 tttgcgcgtt gagttgctgg agattgcgaa tttcgtgggt cagggtatgg cgttcttgtg
    17701 cttctttacc gaagctgtcc tgaacctgac ggcggaaacc gtccagttgt tcacgtagcg
    17761 gcgacaacag gctgttcaga ctctgacggt tttgctcatc aacccggcga ttgctgtgct
    17821 caaaaatacg gttggcgagg ttttcaaact gctcactgag gcgctgctcg ctgttaatca
    17881 tctggcgaat tttatcgtca gcatgttgct gtgcggcttc catccgcgtg gttacttcac
    17941 gcagatcggc ctccagagag gtgttaatac tttgcaggct gcgcacttcg ttattgagta
    18001 actcgcactc tgcacgccag tgctcgcttt gggtaatttg ttgttttgcc gcgcttaact
    18061 ccgcgaccat ctcttcacgt tcagctaatt gctcggcttt ttgctgcgca tgttgataac
    18121 tggcaaacag ccagccaatt gccacaccca ccaacgcaat aactgcgtaa accatgattg
    18181 agaaatccac aacgcctcct gacgcaacaa cttacccgca caaaatagaa ttgtgctgta
    18241 caaacgtcca gttggaaagg catttcctgc gaggcgctac gcaaaaatac aaaaggccga
    18301 acgcgtcggc cttcagacag gagaagagaa ttacagcaga cgacgcgccg cttccaccac
    18361 gattttcacc gcttggcttt cagtttgttt catcgtctca gcattcggga tctcttgctg
    18421 ggtgcggtta acgataacac ccgctaccat accggcacgc aggccctgac ttgcacacat
    18481 ggtcagcagg gttgcagatt ccatttcata gttcattatg cccatcgcct gccactcttc
    18541 catagaacct ttaaagtgac gaactacgcg accagagtaa gtatcgtaac gttcctgacc
    18601 tgggtagaag gtatcagaag aagctgtcac gccaacgtga gttgtcgcgc caatggattt
    18661 cgcagcttca accagcgcag tcgtacattc gaaatcagcg acagccggga attccagcgg
    18721 tgcgaagtgc aggctcgcgc catccagacg gacagacgcc gtggtaacca ggacatcacc
    18781 cacattaata tgcggctgaa tagcgcccgt tgtaccgata cgcaggaagg tgcgaatgcc
    18841 cagctgtgcc agctcttcaa cagcaataga ggtagacggg ccgccgatac cggtagagca
    18901 gacgataaca ggtttaccat ccagctctgc acgccaggta gtgaattcgc ggtgagatgc
    18961 cagcttaacc ggcttatcca tcagcgcggc gatcttttcc acacgatccg ggtcgccagg
    19021 gacgatggca agcgtagccc cttgtaaatc gtttttagtg aggccgagat gaaaaacatc
    19081 agacttggac atatacaact cctctgtgaa tcggtttagt cagaagaagc aaaaagacac
    19141 tttaccgaag ggtttaacat tttttcgtga tactcatcac catgacgcaa atgcgttgca
    19201 taaatttacc caccaaaagt gattcaaatc acataaatca ctcttcattc tgcaggactg
    19261 cataaaagat gagccgattc aggcaatgtg atttgttgca tgctgactat agttaacatt
    19321 gcgcaacatt tttataaggg tacggagaat accatggcaa caacacaaca atctggattt
    19381 gcacctgctg catcgcctct cgcttcgacc atcgttcaga ccctggacga cgcgattgtg
    19441 gcgggcttca cctctatccc ttcacaaggg gataacatgc ctgcttacca tgccagacca
    19501 aagcaaagcg atggcccact gccagtggtc attgtagtgc aggaaatttt tggcgtgcat
    19561 gaacatatcc gcgacatttg tcgccgtctg gcgctggagg ggtatctggc tatcgcacct
    19621 gaactttact tccgcgaagg cgatccgaat gattttgccg atatccccac gttgcttagc
    19681 ggtctggtag caaaagtgcc tgactcgcag gtgctggccg atctcgatca tgccgccagt
    19741 tgggcttccc gcaacggcgg cgatgttcat cgtttaatga tcaccggatt ctgctggggt
    19801 ggacgtatca cctggctgta tgccgcgcat aatccacagc taaaagctgc agtggcgtgg
    19861 tacggcaaac tgacgggcga caagtcgctg aattcaccga aacaacctgt tgatatcgca
    19921 accgatctta acgcgccgat tctcggctta tatggcggtc aggataacag cattccgcag
    19981 gagagcgttg aaaccatgcg ccaggcgctg cgggctgcta atgcgaaagc agagattatc
    20041 gtgtacccgg atgccgggca tgcattcaac gctgattatc gcccgagcta tcatgccgaa
    20101 tctgcgaaag atggctggca gcgtatgctg gaatggttta agcagtatgg agagaagcgg
    20161 agttagtttc ctcttagtct atatttgaga tggtgagtaa acgagtgcgt tttgctgcca
    20221 tctctgtccc ccaaaaaata atcagtatga attgattgga gattacgact ccgctatttg
    20281 accttaagaa agtcggcaag tcatagtcat aaatgagtct cttcaatgga agacacctta
    20341 gagatttacc atcaactccc attcacaaac aaacattaat tattatcaac acattcacat
    20401 taattataaa tcatctcaga ccttcctgat aaaaagatat taaagtgaaa cattttgttt
    20461 ttcacatttt agttcatcac ttttaggcgt aaataaaggt aaaaaaaaca acaatactat
    20521 ttctctttca caaaagaatt aacaaaaggt ttttcaagga tgagtgatat tatttacctg
    20581 accataaccg gagaacaaca aggatgtatt tcctctcgtt gtggaacgag cgcatcaata
    20641 ggtaatcgct ggcagattgg ccatgatgat gaaatcttcg cgttctcgct ctctaatagt
    20701 attacgaata caggcaaagg gtcataattg catggcctta gtttctgtaa attgattgac
    20761 aaaagctccc cgttacttat taacgctatt aacaataacg agcagttgtt catggagttt
    20821 gacttctatc ggatcaatag gtttggtcga tgggagaaat attacaacat tcaactccgc
    20881 ggcgcgctct tatcagcaat caatcacctg tttactgaga acaatctgga tacagaagcc
    20941 atcactgtca gttattaata tatactctcc aggcatctga ttgctaatac tgaatttagt
    21001 tattgggcat ttcccgataa ctacaaccgt ctgtttatac cacggccaaa aactaaagct
    21061 ggtaatggtt tcaaaacact taatagtaaa gcggttggca gactgcttgc tgctgtcggc
    21121 atatacaatg ggaatatcga aggattcagg gaaacagcag acaagttggg gggagatgca
    21181 cctgctggtt atgatcaatt tatggataac aaagggttac tcatcgctgg tgcttcaata
    21241 gttgctggag cagttctggg gagaatgaga ctccctgatt tggaatcact tgagcatttc
    21301 ggagccagag gagcagtatc aggaagacca tttaatcccg agcttgccgg tggtccaata
    21361 gaaaacctga cgacagatgg cgtaacaatc aacagggaag gtatcgctat tgttgaaaaa
    21421 catatagcca gatttggtca tgatcctgta aatgaagtga tgatcaatag actgaaagac
    21481 attgagaaag gaaaaatccc cccagaacaa gtagatctta acttctatac tcatgagtgt
    21541 cgggaatatc agcgttactg taatcttggt tgggaaacag gacaacctga tggtgacgca
    21601 ggctatgcac tctggaatca tactggattt gcccctatat ttccagacat ctgttatcac
    21661 ttaacccatt acaagcccgc tgcc
//
LOCUS       DAQSWQ010000069        21643 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.069, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000069 DAQSWQ010000000
VERSION     DAQSWQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.069"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="R8G18_002717"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="R8G18_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HEE9796371.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="R8G18_002718"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="R8G18_002718"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEE9796372.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="R8G18_002719"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="R8G18_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HEE9796373.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="R8G18_002720"
     CDS             complement(3717..4511)
                     /locus_tag="R8G18_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEE9796374.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="R8G18_002721"
     CDS             4649..4990
                     /locus_tag="R8G18_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HEE9796375.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="R8G18_002722"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="R8G18_002722"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HEE9796376.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="R8G18_002723"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="R8G18_002723"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEE9796377.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="R8G18_002724"
     CDS             8906..10198
                     /locus_tag="R8G18_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEE9796378.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="R8G18_002725"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="R8G18_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEE9796379.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="R8G18_002726"
     CDS             complement(10731..11189)
                     /locus_tag="R8G18_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEE9796380.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="R8G18_002727"
     CDS             complement(11186..12103)
                     /locus_tag="R8G18_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HEE9796381.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="R8G18_002728"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="R8G18_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HEE9796382.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="R8G18_002729"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="R8G18_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEE9796383.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="R8G18_002730"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="R8G18_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HEE9796384.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="R8G18_002731"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="R8G18_002731"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HEE9796385.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="R8G18_002732"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="R8G18_002732"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HEE9796386.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="R8G18_002733"
     CDS             16063..16749
                     /locus_tag="R8G18_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEE9796387.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="R8G18_002734"
     CDS             16746..19160
                     /locus_tag="R8G18_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9796388.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="R8G18_002735"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="R8G18_002735"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEE9796389.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="R8G18_002736"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="R8G18_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5013 a         5686 c         5800 g         5144 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAQSWQ010000070        21003 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.070, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000070 DAQSWQ010000000
VERSION     DAQSWQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.070"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..170)
                     /locus_tag="R8G18_002737"
     CDS             complement(<1..170)
                     /locus_tag="R8G18_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796390.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="R8G18_002738"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="R8G18_002738"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEE9796391.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="R8G18_002739"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="R8G18_002739"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEE9796392.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="R8G18_002740"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="R8G18_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEE9796393.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="R8G18_002741"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="R8G18_002741"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEE9796394.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="R8G18_002742"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="R8G18_002742"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEE9796395.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="R8G18_002743"
     CDS             4288..5151
                     /locus_tag="R8G18_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEE9796396.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="R8G18_002744"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="R8G18_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEE9796397.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="R8G18_002745"
     CDS             6489..7106
                     /locus_tag="R8G18_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEE9796398.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="R8G18_002746"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="R8G18_002746"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HEE9796399.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="R8G18_002747"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="R8G18_002747"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEE9796400.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="R8G18_002748"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="R8G18_002748"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEE9796401.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="R8G18_002749"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="R8G18_002749"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HEE9796402.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="R8G18_002750"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="R8G18_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HEE9796403.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="R8G18_002751"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="R8G18_002751"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEE9796404.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="R8G18_002752"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="R8G18_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEE9796405.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="R8G18_002753"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="R8G18_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HEE9796406.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="R8G18_002754"
     CDS             18072..19781
                     /locus_tag="R8G18_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEE9796407.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="R8G18_002755"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="R8G18_002755"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEE9796408.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5718 c         5385 g         4817 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggct
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaactt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggca tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcacc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAQSWQ010000071        20241 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.071, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000071 DAQSWQ010000000
VERSION     DAQSWQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.071"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            690..1460
                     /locus_tag="R8G18_002756"
     CDS             690..1460
                     /locus_tag="R8G18_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HEE9796409.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1451..1924
                     /locus_tag="R8G18_002757"
     CDS             1451..1924
                     /locus_tag="R8G18_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HEE9796410.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2032..2571
                     /gene="dnaT"
                     /locus_tag="R8G18_002758"
     CDS             2032..2571
                     /gene="dnaT"
                     /locus_tag="R8G18_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HEE9796411.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2574..3311
                     /gene="dnaC"
                     /locus_tag="R8G18_002759"
     CDS             2574..3311
                     /gene="dnaC"
                     /locus_tag="R8G18_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HEE9796412.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3360..3854
                     /locus_tag="R8G18_002760"
     CDS             3360..3854
                     /locus_tag="R8G18_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HEE9796413.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4108..6399
                     /gene="opgB"
                     /locus_tag="R8G18_002761"
     CDS             4108..6399
                     /gene="opgB"
                     /locus_tag="R8G18_002761"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HEE9796414.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="R8G18_002762"
     CDS             complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="R8G18_002762"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HEE9796415.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7699..8613
                     /locus_tag="R8G18_002763"
     CDS             7699..8613
                     /locus_tag="R8G18_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEE9796416.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8828..10189
                     /locus_tag="R8G18_002764"
     CDS             8828..10189
                     /locus_tag="R8G18_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9796417.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10238..11893)
                     /gene="tsr"
                     /locus_tag="R8G18_002765"
                     /pseudo
     CDS             complement(10238..11893)
                     /gene="tsr"
                     /locus_tag="R8G18_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12012..12458)
                     /gene="hpaR"
                     /locus_tag="R8G18_002766"
     CDS             complement(12012..12458)
                     /gene="hpaR"
                     /locus_tag="R8G18_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HEE9796418.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12730..14019
                     /locus_tag="R8G18_002767"
     CDS             12730..14019
                     /locus_tag="R8G18_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HEE9796419.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14016..15482
                     /gene="hpaE"
                     /locus_tag="R8G18_002768"
     CDS             14016..15482
                     /gene="hpaE"
                     /locus_tag="R8G18_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HEE9796420.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15484..16335
                     /gene="hpaD"
                     /locus_tag="R8G18_002769"
     CDS             15484..16335
                     /gene="hpaD"
                     /locus_tag="R8G18_002769"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HEE9796421.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16345..16725
                     /locus_tag="R8G18_002770"
     CDS             16345..16725
                     /locus_tag="R8G18_002770"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HEE9796422.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16836..17639
                     /gene="hpaH"
                     /locus_tag="R8G18_002771"
     CDS             16836..17639
                     /gene="hpaH"
                     /locus_tag="R8G18_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HEE9796423.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17650..18438
                     /gene="hpaI"
                     /locus_tag="R8G18_002772"
     CDS             17650..18438
                     /gene="hpaI"
                     /locus_tag="R8G18_002772"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HEE9796424.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18613..19989
                     /gene="hpaX"
                     /locus_tag="R8G18_002773"
     CDS             18613..19989
                     /gene="hpaX"
                     /locus_tag="R8G18_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HEE9796425.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19999..>20241
                     /locus_tag="R8G18_002774"
     CDS             19999..>20241
                     /locus_tag="R8G18_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HEE9796426.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4871 a         5432 c         5171 g         4767 t
ORIGIN      
        1 tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat
       61 cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt attcaggata
      121 ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat
      181 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
      241 agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt
      301 gttaggaata cttaatgaaa aacagaaatc acattcattt aatttataat taaatgatat
      361 cccttcccgc agaatgaaaa atcacacaac caatatttat tcaatgaaat ctgatggttt
      421 taacttgtta acattaaaaa acgtgcccgg ttatctatta tcctgattcg taaaggccaa
      481 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
      541 tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt
      601 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      661 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      721 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      781 atgagctttc ctcacgactg ggtcgggcgt tggggatgga cagcgtcgaa agttctatct
      841 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      901 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      961 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
     1021 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
     1081 gcaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
     1141 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccgcagatc aacttttgcc
     1201 tcaccgcttt cgccgccacc accatttccg gattgctttt gcaacttccc actttcagca
     1261 atacacccac tatcgcgatg gccgccagcg ttctgctgct ggtgccggga tttccgttga
     1321 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
     1381 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
     1441 ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata tgatcctcgc
     1501 cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cattacgctg
     1561 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1621 gaatattgag tggtcaacct ttatggcctc catgctggtc ggcaccattg gtattcaatg
     1681 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1741 cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agttaggtta
     1801 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1861 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1921 ataaaattgc ctcgcttaaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1981 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     2041 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     2101 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     2161 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     2221 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     2281 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     2341 ctggcggcgc tatggggtgt ggcactaaga gagccggtga ccaccgaaga actggcctca
     2401 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2461 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2521 aatacggtca gcgaacctga cagtcaaatt ccaccaggat tcagagggta acgatgaaaa
     2581 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2641 tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc cgctccgccg
     2701 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2761 ggccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2821 atgcgttaag caaagcgcgc cagtacgtcg aagagtttga cggcaacatc gccagcttca
     2881 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2941 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     3001 aagatacctt caggaatagc ggtaccagtg aagaacaact gcttaacgat ctgagcaacg
     3061 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     3121 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     3181 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     3241 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta
     3301 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     3361 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3421 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3481 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3541 gcagcgggaa ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat
     3601 actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3661 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3721 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3781 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3841 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3901 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3961 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     4021 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     4081 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     4141 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     4201 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     4261 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     4321 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     4381 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     4441 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4501 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4561 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4621 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4681 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4741 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4801 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4861 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4921 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4981 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     5041 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     5101 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     5161 gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc
     5221 gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     5281 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     5341 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     5401 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     5461 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5521 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5581 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5641 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5701 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5761 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg
     5821 tgtgtctcgc aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca
     5881 tggcagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat
     5941 gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc
     6001 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagtg ggatttcccg tccagagtcg
     6061 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     6121 ctgccaaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     6181 cctattccgg tacgcgtagg caatgaagaa caaacccttg tgctgggcaa tgaagtgacc
     6241 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     6301 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     6361 ggcatggtgg aaattaaagt ggtggaacgt gaaggataat tcctgactga tgtgtaggtc
     6421 gcataaggtg cgacctacac accttctgcg gcctacaaaa catcattgag aaacagaaaa
     6481 aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca
     6541 gaaagtaatt acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc
     6601 tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcagcagtga tttttccttc
     6661 cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tacccatcat
     6721 cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc
     6781 gacaaatacc accgtgccgc cgtgacgaat caaattcacg gtgttattca tcgcatgttg
     6841 attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg
     6901 cgcgtcaaaa tcttcggctg acgggtccag taaaggtaat tccagacgcg ttgccacatg
     6961 ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat
     7021 tgccgccgcg cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc
     7081 aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgctgccgc
     7141 ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact
     7201 aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc
     7261 gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc
     7321 atttttaaga tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc
     7381 taaaacacgt ggataactaa aaaatggttg atttccaccc caggcatgaa tatcggtacc
     7441 gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc
     7501 gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat
     7561 gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga
     7621 acggaaaatc tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg
     7681 actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag
     7741 gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg
     7801 gcggaaatgt acaacatcag ccgcaccaca gtgcgtcata ttctcagcca cttacgcgaa
     7861 tgcggcgtct tgacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccatgat
     7921 gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct
     7981 tttttcacga tgataaacca gcgccagtta cgcccagggg aaacgttttc tgaactgcaa
     8041 ctggctcggg ccgcgggtgt cagcccggtt gtagtaagag aatatctttt aaaattcggc
     8101 cgttataatc ttattcacag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa
     8161 tcctacgccg agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac
     8221 tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt
     8281 catcggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga
     8341 gattttcact ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag
     8401 atcatctcag tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc
     8461 aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg ccgcagtgac
     8521 cttgatgcca cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc aatgattcga
     8581 tccattaatg agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta
     8641 gcgggtgcac ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac
     8701 aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc
     8761 gttaatgtca accttgcgta attcacaatt acgtaaacaa ttaaaattat aaatacagga
     8821 gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc
     8881 gtcggcagat attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg
     8941 cattcaaacc accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg
     9001 cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga
     9061 aatcggcgct ttgctctccg tgttttcact cgcttacggg attgcgcaac ttccttgcgg
     9121 cccactattg gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc
     9181 actgttccag gcaatgtctg gcatggtgca caactttacg cagttcgtgt tggtgcgtat
     9241 cggtatgggg attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg
     9301 gttcaacatc aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg
     9361 cgttgccgta agcccaccga ttctggcggc gatgatgctg gtgatgggct ggcgcgggat
     9421 gtttattacc attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg
     9481 caaccgcgag cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt
     9541 caatgcccgc cgagatccgc tcagttttgc cgaatggcgc agcctgttcc gtaaccgtac
     9601 aatgtgggga atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc
     9661 ctggcttcct ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat
     9721 ggcggctatc cctttcctgt ttggggctgc cgggatgctg gtcaacggtt acgttactga
     9781 ctggctggtc aaagggggaa tggctccgat taaaagccgt aagatctgca ttattgccgg
     9841 gatgttctgt tctgccgcct ttacgctggt cgtaccgcaa gcgacaacat ccatgacagc
     9901 ggttctgctg attggcatgg cactgttctg tattcacttt gccggaacat cctgctgggg
     9961 cttgatccac gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt
    10021 tgccagcttc atctgcgcct cttttgcgcc gatcattact ggttttattg ttgataccac
    10081 ccactcattc cgtctggcac taatcatctg cggttgcgtc accgcagcgg gggcactggc
    10141 gtacatcttc ctggttcgtc agccgatcaa cgacccacgt aaagattaat ctccttatgc
    10201 ccgataacat tttgcttatc gggcattttc atggcgatta aaatgtttcc cagttctcct
    10261 cgctatctgc cacggccatt ttacgcggcg cagctggcgt cacggttttt accacagccg
    10321 atgtttcacg ctgctgttgc tgaatccgga acactgccac tgcttcggtc aggcgactgg
    10381 cctgctcttc cagcgcggcg gcggcagcgg cagactcttc caccagcgcg gcgttctgtt
    10441 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10501 catcagaagc agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg gcgctgacaa
    10561 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10621 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctgcgc tgggccagat
    10681 tacgcacttc tcccgcgacc accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct
    10741 caaccgccgc gttcaaagcc agaatattgg tctggaaggc aatgccgtca attacgctga
    10801 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10861 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taacgccaga tggctggcct
    10921 ggcgcgcatt ttcggcgttc tgcttcaccg ttgcggtcag ttactccatg ctggctgccg
    10981 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg
    11041 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    11101 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11161 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11221 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11281 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11341 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11401 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11461 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11521 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11581 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11641 aaccgctacc aatattgttc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11701 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11761 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaagaac agaccgcctg
    11821 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11881 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11941 aaaaacttta tgaaaaggtg taacccacca tccatggaaa aaccaggcgc tggttatcag
    12001 atactaaaaa gttattcatt atcgcccggg atggcttctt gtcgggaatt gcccagagca
    12061 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12121 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12181 ttggttagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12241 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12301 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12361 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12421 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12481 aaatcattaa tatagaaaca gttaatcgtc acgttaccgg gactgcgctg cccctgcaat
    12541 tcctgatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12601 tctttaaatg taaatagttt gttaattaga tcacatttac atcacttact tttgccaaac
    12661 tacgcaatcg ggaataaaag caatcattaa catattaatg attaagatga tcgaatccga
    12721 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12781 gcatggcagg aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg
    12841 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12901 gaaaaggtac tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc gacgaaagta
    12961 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    13021 ccggaagaga gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    13081 attggcgaaa ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac
    13141 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc gcagttgctg
    13201 agcgccctga gcgaatttgc cacgctgaat ccaggcgacg ccattctgct cggcacgcca
    13261 caggcgcgcg tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga aggtttcccg
    13321 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcacaagag cttcccaacg
    13381 cagccacacc cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13441 gaactggaat ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc aaataccctc
    13501 actggcgata accagacctc cgtgcgtcca aacaatattg aatacatgca ctatgaagcg
    13561 gagctggtgg tagttattgg caaacaggcg cgtaacgtca gcgaagccga tgccatggat
    13621 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gtgactatct ggaaaactac
    13681 taccgcccta acctgcgggt aaaaagccgc gacggactga cgccgatgct ttcaaccatc
    13741 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13801 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13861 tacttaagtg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacacccaaa
    13921 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13981 ctggtgaacc gaattgtgag tgaggataca gcgaaatgaa aaaagtaaat cattggatca
    14041 acggcaaaaa tgttgcaggt aacgactact tccagaccac caatccggca acgggtgaag
    14101 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14161 aagaggcgtt cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14221 gtctgggcga tctgatcgac cagaacgtgc cagagatcgc cgcgatggaa accgcggaca
    14281 ccggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttcc cacaactttg
    14341 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14401 tgctcaacta cacgctggtg cagccggtgg gcgtttgtgc gctggtatcg ccgtggaacg
    14461 taccgtttat gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14521 tactgaaaat gtcggaactc tccccgctga ccgctgacag cctgggtgag ctggcgctgg
    14581 aagccgatat tccggcaggc gtgctgaacg tggtacaggg ctacggcgca accgcagggg
    14641 atgcgctggt tcgtcatcat gacgtacgtg ccgtgtcgtt caccggcggt acggccaccg
    14701 ggcgcaacat catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat
    14761 cgccggtgct gatttttgaa gatgccgata ttgaacgcgc gctggacgcc gccctgttca
    14821 ccatcttctc gatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaaa
    14881 gcatctaccc ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14941 atccgaacga tccgaatacc caggttggcg cgcttattag ccagcagcac tgggaaaaag
    15001 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg
    15061 ataaaccgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaaccgtgc
    15121 tggcagacgt tgataaccgt atgcgagtcg cccaggaaga gattttcggg ccggtcgcct
    15181 gcctgctgcc gtttaaagac gaagccgaag gcttacgtct ggcaaacgac gtggagtacg
    15241 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15301 ttgaagctgg catggtgttc gtcaacaccc agaacgtgcg tgacctgcgc cagccatttg
    15361 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15421 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15481 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatcactct
    15541 gaactgccag ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc
    15601 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg
    15661 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15721 gagctgccgc attttatccg cgatatgacc tacaactacg agggcaaccc ggagttgggg
    15781 cagcttattg ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca caacattccc
    15841 agcctgaaac tggaatacgg cacgctggtg ccgatgcgct acatgaatga agacaagcac
    15901 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15961 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    16021 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    16081 tacacccgcg agttcgaccg ccagatggac gagcgcgtgg tgaagttgtg gcgcgaaggc
    16141 cagttcaaag agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16201 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16261 gtggagttta tcaccgagct attcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16321 ccgcttcccg cgtaaggacg ttttatgccg cactttatcg ttaaatgcag tgacaacatc
    16381 cgcgaagaag ccgacctgcc ggggttgttc gccaaagtga atccgacgct ggcagccacg
    16441 ggtatttttc cgctggcggg tattcgcagc cgcgtgcatt gggtcgatac ctggcagatg
    16501 gccgacgggc agcgtgatta tgccttcgtg catatgacgt tgaaaatcgg cgcaggtcgc
    16561 agcctggaaa gccgccagca ggcgggtgaa atgctgtttg aactgattaa aacgcacttc
    16621 gccgccctga tggagagccg cctgctggcg ttgtcgtttg agattgaaga gctgcatccg
    16681 acgctgaatt ttaaacaaaa caacgtgcac gcattgttta agtagcgcgc agattgcccg
    16741 gtggcgctgc gcttaccggg cctacaaaac cccaaaccgt acaccgtagg ccggataagg
    16801 cgcagccgca tccggcaatg ccacaggata tcgctatgtt cgataaacac acccacaccc
    16861 tgatcgccca gcgtctggat caggcagaaa aacagcgcga acagatccgc gcgatctcgc
    16921 tggattaccc ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa tgggttcgcc
    16981 tgaaaatcgc cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc tcgaaagcga
    17041 tgcaggcaag ctcgcagatc agcgaaccgg attacggcgc gctgctggat gacatgttct
    17101 tccacgatgg cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc
    17161 tggctttcgt gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca
    17221 acgccacgga ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc cacaacatcg
    17281 atccggaaac ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac gccgccaacg
    17341 ccggggtgat cctcggcggt cgtccgatca aaccagacga actggatctg cgttggatct
    17401 ccgccctgat gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct ggcgtgctga
    17461 atcatccggc aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat gacgtacaac
    17521 tggaagccgg gcaaatcatt ctcggtggtt cgttcacccg cccggttccg gcgcgtaagg
    17581 gcgacacctt ccacgtcgat tacggcaaca tgggttccat tagctgccgc tttgtttaag
    17641 gagagaacga tggaaaacag ctttaaagcg gcgctgaaag caggccgtcc gcagatcgga
    17701 ttatggctgg ggctgagtag cagctacagc gcagagttac tggccggagc aggattcgac
    17761 tggttgttga tcgacggtga gcacacgccg aataacgtgc aaaccgtgct cacccagcta
    17821 caggcgattg cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg
    17881 caaatcaaac aactgctgga cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac
    17941 gctgacgaag cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg tattcgcggt
    18001 gtgggcagtg cgctggcccg cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa
    18061 gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat gaagaactta
    18121 ccgcagattc tggacgtgga aggcgtcgac ggcgtgttta tcggcccggc ggatctgagc
    18181 gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc cattgagcag
    18241 gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa
    18301 ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc
    18361 ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtgcgcaggc caccgccgtg
    18421 aagcccggcg tgtattaatg ccggatgcgc tgcgcttatc cggcctacac tcgcaccaaa
    18481 ccgtaggccg gataaggcgt ttacgccgca tccggcaaaa agccgtacca aatcgcggac
    18541 cggataaggc gttacgccgc atccgccata aaccttgtcg taccctacaa aaatcccatt
    18601 agaggaagaa aaatgagcga cacctcacct gccataccgg agagtatcga tccggcgaat
    18661 cagcataaag cgctgactgc cggacaacag gcggttatta agaagctatt tcgccgcctg
    18721 agcgtctttc tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa catcggcttt
    18781 gccggactca cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg cctcgctacc
    18841 accctgttct atgccgctta tgtcatcttc ggcattccca gcaacattat gctgagtatt
    18901 gtcggtgcgc ggcgctggat cgccaccatc atggtgctct ggggcatcgc ctctactgcc
    18961 accatgtttg ccactggccc caccagcttg tacgtactgc gtatactggt tggcattacc
    19021 gaagccggct ttctgcctgg tattctgctg tatttaacct tctggtttcc ggcctatttc
    19081 cgcgcccgtg ccaacgcctt gtttatggta gcaatgccgg taacgacagc gttgggctcg
    19141 atcgtttccg gctacatttt gtcgctggat ggcgtcatgg cattaaaagg ctggcagtgg
    19201 ctgtttttgc tggaaggctt cccgtcggta ttactcggcg tcatggtgtg gttctggctt
    19261 gatgactcac cggacaaagc taagtggctg acgaaagaag acaaaaaatg cctgcaagag
    19321 atgatggata acgatcgtct gacgctggtt cagccagagg gagccatcag ccaccacgcc
    19381 atgcaacaac gcagtatgtg gcgggagatc ttcactccgg tggtgatgat gtataccctg
    19441 gcgtatttct gcctgaccaa cacacttagt gcgatcagca tctggacacc gcagatcctg
    19501 caaagtttta atcagggcag cagtaatatc accatcggca tgctggccgc cgtaccgcag
    19561 atttgtacca ttctcgggat ggtctactgg agccgtcact cagatcgccg ccaggaacga
    19621 aggcatcaca ccgcccttcc ttatttgttc gctgccgcag gttggttact ggcttcggca
    19681 actgatcaca acatgatcca gatgctgggg atcattatgg cttcgaccgg atcattcagc
    19741 gcaatggcga ttttctggac aacaccggat cagtccatca gcctgcgggc acgagcgatc
    19801 ggtattgcgg tgatcaacgc cactggcaac attggttcag cgttaagtcc gtttatgatc
    19861 ggctggttga aagatttgac cggcagcttt aacagtggat tgtggtttgt tgccgcgctg
    19921 ctggtgattg gtgcggggat tatctgggcg attccaatgc agtcctcccg tccgcgagcg
    19981 accctgtaag gaacgacgat gtgtgaccgt cagattgcca atattgatat cagcaaagag
    20041 tacgatgaaa gcctgggcac ggacgatgtg cattatcagt cgttcgcccg catggcggca
    20101 ttttttggcc gccatatgct gccacatcgc cacgaacagt actttcagat gcatttcctc
    20161 aatagcggac agattgagct acagcttgac gatcatcgct actcggtgga agcgcccctg
    20221 tttgtcctga cgccgccgtc a
//
LOCUS       DAQSWQ010000072        20061 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.072, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000072 DAQSWQ010000000
VERSION     DAQSWQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.072"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="R8G18_002775"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="R8G18_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEE9796427.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="R8G18_002776"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="R8G18_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEE9796428.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="R8G18_002777"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="R8G18_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEE9796429.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="R8G18_002778"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="R8G18_002778"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEE9796430.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="R8G18_002779"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="R8G18_002779"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEE9796431.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="R8G18_002780"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="R8G18_002780"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEE9796432.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="R8G18_002781"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="R8G18_002781"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HEE9796433.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="R8G18_002782"
     CDS             5315..6439
                     /locus_tag="R8G18_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEE9796434.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="R8G18_002783"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="R8G18_002783"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HEE9796435.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..8261
                     /gene="waaO"
                     /locus_tag="R8G18_002784"
                     /gene_synonym="rfaI"
     CDS             7245..8261
                     /gene="waaO"
                     /locus_tag="R8G18_002784"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HEE9796436.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8278..9273
                     /locus_tag="R8G18_002785"
     CDS             8278..9273
                     /locus_tag="R8G18_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HEE9796437.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9283..9975
                     /gene="rfaY"
                     /locus_tag="R8G18_002786"
     CDS             9283..9975
                     /gene="rfaY"
                     /locus_tag="R8G18_002786"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HEE9796438.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10001..11026
                     /locus_tag="R8G18_002787"
     CDS             10001..11026
                     /locus_tag="R8G18_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HEE9796439.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11111..12094
                     /locus_tag="R8G18_002788"
     CDS             11111..12094
                     /locus_tag="R8G18_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEE9796440.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12140..13393
                     /locus_tag="R8G18_002789"
     CDS             12140..13393
                     /locus_tag="R8G18_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEE9796441.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13463..14434)
                     /gene="rfaC"
                     /locus_tag="R8G18_002790"
     CDS             complement(13463..14434)
                     /gene="rfaC"
                     /locus_tag="R8G18_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HEE9796442.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(14438..15484)
                     /gene="rfaF"
                     /locus_tag="R8G18_002791"
     CDS             complement(14438..15484)
                     /gene="rfaF"
                     /locus_tag="R8G18_002791"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HEE9796443.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15494..16426)
                     /gene="rfaD"
                     /locus_tag="R8G18_002792"
     CDS             complement(15494..16426)
                     /gene="rfaD"
                     /locus_tag="R8G18_002792"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEE9796444.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16640..17836
                     /gene="kbl"
                     /locus_tag="R8G18_002793"
     CDS             16640..17836
                     /gene="kbl"
                     /locus_tag="R8G18_002793"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEE9796445.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17846..18871
                     /gene="tdh"
                     /locus_tag="R8G18_002794"
     CDS             17846..18871
                     /gene="tdh"
                     /locus_tag="R8G18_002794"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEE9796446.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19110..20012
                     /locus_tag="R8G18_002795"
     CDS             19110..20012
                     /locus_tag="R8G18_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEE9796447.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMW"
BASE COUNT         5409 a         4372 c         4741 g         5539 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atgctaaata caatactcaa tttagtttaa
     7981 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
     8041 atgtcggccc gacaaaaccc tggcattact gggccggtta tccaagtgcg caacctttta
     8101 tcaacgccaa agaagcatcg ccctggaaaa atgaaccgtt tatgcggcca attaattcaa
     8161 actatgctcg ttattgcgcc aagcataatt ttaaacaaaa tgaaccaatt aacgggataa
     8221 tgaattatat ttattatttt tatttaaaga taataaaatg atattgagaa aagttaaatg
     8281 aatgaattta taaaagaacg gttttcgtat ttagcagata ataaaaaaga aaacgcccca
     8341 gaactaaatg tttcctacgg tatcgataag aattttttgt atggtgctgg cgtttcaatt
     8401 tcttccgttt tgattaataa ttcagatatt aattttgtct ttcatgtttt cactgattat
     8461 gtggatgatg attatttaaa gtcatttaat gaaacagcaa aacaatttaa tacctcaatt
     8521 attgtatatt taattgaccc aaaatacttt gctgatctgc cgacgtcaca gttttggtcg
     8581 tacgcgacat acttcagggt attgtccttt gaatatctga gtgaaagtat ttccacactg
     8641 ctgtatctgg atgccgatgt cgtttgtaaa ggaagtctga agcctctcac agaaattata
     8701 tttaaagatg agtttgctgc ggtcattcct gacaatgtta gtactcaggc ggcatgtgca
     8761 aaacgcctca acattcccga gatgaatgga cgttatttca atgcaggcgt tatctatgtc
     8821 aatcttaaaa aatggcatga agcaaatttg acaccgtatt tactcacgct tttacgaggg
     8881 gaaactaaat atggctctct taaatattta gatcaggatg cgttgaatat cgcatttaat
     8941 atgaataata tctacctcgc gaaggatttt gatactattt ataccctgaa aaacgaactt
     9001 catgatcgta gtcatcgaaa gtatcagcaa accattaccg ataaaacagt attgattcac
     9061 tatacaggga taactaaacc atggcatagc tgggctggtt atccgtctgc atcatacttt
     9121 aatatcgcgc gtgaacaatc tccctggaag aaatatcctc ttaaagaggc gcggactgtt
     9181 gcagaaatgc agaaacaata taagcatctg tttgcccatg gtgagtatat taaaggcata
     9241 acttcattaa ttaagtacaa gcttaagaaa taaattactc ttatgattac aagtatacgc
     9301 tatcgcggct tctcatttta ttacaaagat aatgataata aatataaaga aatctttgat
     9361 gaaatactgg cctataattt taaaacagta aaagtattac gtaatattaa tgataccaaa
     9421 gtatcattga ttgatacgaa atatggtcgc tatgttttta aagtctttgc cccgaaaacg
     9481 aaaaggaatg aacgtttttt aaaatctttt gtaaaaggtg actactatca gaatttgatc
     9541 gttgaaaccg atcgtgtcag aagtgcaggt cttacttttc ctaatgattt ttattttctg
     9601 gctgagcgta aaattttcaa ctatgccagt gtttttatca tgcttatcga gtatgtcgag
     9661 ggcgtagagc tcaatgatat gccgatcatt ccagaagatg ttaaagcaga aattaaggct
     9721 tcaatggaaa aattgcatgc cttaaatatg ctatcgggcg acccacaccg gggtaacttt
     9781 atcgttagta aagatggtgt caggataatt gacctatccg gtaaaagctg tacggcagag
     9841 cgtaaagctc gtgatcgtct ggccatggaa cgtcacctgg gtattgccaa cgaaataaaa
     9901 gattatggct actattcagt aatctacaga accaaactac gcaaatttat taaaaaatta
     9961 aaaggcaaag cgtaaaccac acagtcaaaa cggaaccaac atggatttat tagctgagag
    10021 tattactgaa gtcgctgtct ctggggaaat tgctaacacc gatcgtgtgt taaatatcgc
    10081 ttacggtatt gaccgtaact ttttatttgg tgcggcagta tctatgcaat cagttgttat
    10141 gcataatccg gaccttgcgg ttaagtttca tctctttacc gactacattg atgaagatta
    10201 tctacaacgt gttaatgctt ttaccagcaa aaatgctaac gttgaagtaa gaatttataa
    10261 agtctccagt gcctttattg atatcttccc cagcctgaaa cagtggtctt atgcaacatt
    10321 cttccgttta gttgcgttcc agtatctgag tgaaactatt gaaaatctgt tatatatcga
    10381 tgctgatgtc atctgtaaag gctcattagc tggattgctt gatattaatt ttgatgggga
    10441 taagtttgca gctgttatta aagatgtgcc ttttatgcag gaaaaaccag cgaagcgtct
    10501 ggctatagag ggacttccag ggaattattt caacgccggt gtagtatatc tgcagcttga
    10561 agcatgggcg aaaaatgatt ttatgaataa agccattgct atgctggcaa gtgacccgca
    10621 gcacacgaag tataaatgcc ttgatcagga tattttaaat attctgttct ttggtcattg
    10681 tatttttatt agcggcgatt atgattgctt ttatggcatc gattatgagt taaaaaataa
    10741 aagcgatgaa gattataaaa agaccattac cgatgatact aagctgattc attatgttgg
    10801 cgtaacgaag ccctggaacg actggacgaa ttatccctgc cagaagtatt ttaatgaggc
    10861 ttatcaggct tcttgctgga atgatgtggc gtttattcca gccacgaatg aaaagcagta
    10921 tcaagtgaaa tatcaacatg caaagaaaaa tggtgatacg tttaacgctt ttatttactt
    10981 tattaaattt aaattaaata agtataaaag aaaactattt ggataataat aaagatcatg
    11041 gatgtctgag ttacaataat aattaaggat gagtactatc atattttatt agttcaggca
    11101 agagaacacg atgagcaatg attacccttt agtatccata ataataccga cgtataattc
    11161 atctgattac attactgaaa ctctaacgaa attagaaaaa caaacttacc caaattttga
    11221 aattgttatt gttaatgatg gttctaaaga taacacatca aacgttttga gagagtatgg
    11281 gttaacccac tctcgattaa ttattatcaa taaagaaaat ggcggtgttt cgtctgccag
    11341 gaatacaggt atccgcaagg cgcaaggaca gtttatatgt tttatggatg atgatgatga
    11401 gatagatcct aactatctgc tgaagatgta taccagacaa catgagacgg gaggggatgc
    11461 gatttattgt gggctttatg gccattatat aaaagatggt gttacttact cacctataaa
    11521 tacagagttt aatgaaggat ctttactttt cgactttttt tataaaaagg ttagattcca
    11581 tatagggtgc ttgtttataa gaaaacaact tctggaagag aataatcttt tttttgatga
    11641 agatttacga ctaggagaag atctggattt tatctatcga ctgctaatta catgcgatat
    11701 gtatgcggtt ccatattata tgtataagca taactataga gaaaattcct taatgaactc
    11761 atgtagaacc atcactcatt atcgacatga gtcatttgcg cacgaaagaa tctactcttc
    11821 tgtgatgcag ttatacaaag gtaacaggaa agaagaaatt catacattat tgagtcaaaa
    11881 tagagcttat cataaaactc gttatttatg gaatgttcta cttaatggtg attttgaact
    11941 attaaatcaa ttagttgaaa gcaatgaaaa agaattgaat gattgtaatc tttctggcaa
    12001 gagagataaa agacgagcaa aaatattagc atcaaaaaat tatattctct ggaggatggt
    12061 aagactggta aataggaaaa agaataaacg ttagatcttg cattaaataa tccgtcctcg
    12121 tagcataggt tgaaactata tgtcattttg ttggaatgaa attaattctg gtataaagtc
    12181 tttaattctc atattatgta ttttttcttt gatgactttg tctttatggg atgatgttgc
    12241 aacaaagttt cttcatgcag ctggaattat atctgcattg tattttctaa taacaccaaa
    12301 aaaaacaata actaacaatc ctactttgtt aattttcatc tcattatgtc ttttgggaat
    12361 cataaatatc atctggtatt cacattataa agtttcaggc tctgtttata ccaatgcata
    12421 tcgtggccca atggaaactg ggaaaattgc cttgtgtagt gcttttattt tcttggttct
    12481 ttttgctaaa aatgagttga gaacaaaaat aaaatttggg aaactaattc tgttcgcatc
    12541 cctggcaacg cagttacttt tttttgcgca tgccatgtgg caacatttct atttaaacgt
    12601 tgaccgtgtt gcattatcag cttcccacgc tacaacagca ggctacatca tccttttccc
    12661 ttctttactg gcatcaattc tcattttaaa atccgacttt agacataaaa caacattata
    12721 tacaattaac ttcatgctta gcttatgtgc tgtcatagta actgaaacgc gtgcagccat
    12781 attagtgttt ccattctttg cgttactatt aatcgtaatg gatagttata ttaataagcg
    12841 aattaattat aagttatatt gttttattgc gattgcatta ttagcaggtg tattttcttt
    12901 taaagataca ttgcttatga gaatgaatga cttaaataac gatttagtta attattcgca
    12961 tgataacacc agaacttcag tcggtgcccg tctggcaatg tatgaagttg gcttaaaaac
    13021 atattctcca ataggacaat cactggaaaa acgagcggaa aaaatacatg aactagaaga
    13081 aaaagagcct agattgagtg gcgctttacc ctatatagat tctcatttgc ataacgatct
    13141 catagatacg ttatcaacgc gtggtattcc tggagttgta ttaacaattt tagccttttc
    13201 agcaatactc atatatgcct taagaactgc taaagaacct tatattttaa tcttgctttt
    13261 ttcactactg gtagtaggcc taagtgatgt aatactcttt tctaaaccgg ttccgactgc
    13321 tgtgtttgtc accataatat tgctttgtgc ttattttaaa gcacaatcag accaatgttt
    13381 attagataaa taatgaatat gtgaaataaa atcagcaagg aggctgattt tatttcaaaa
    13441 ttatctatta aggggttgta atttaaatca tagcagcgtt ttcttcaatg aatcgcttaa
    13501 cagcatttgc tgtcaattga gacaactcat tccccggagc cctacaaacc atctgattct
    13561 tcccatatcc accaattaat cccggatcgg ttggtccata aaccgtgata ttgggtctat
    13621 ccagtgccgc cgttaaatgg cttaaccccg tatccaccga cactacaaat ttagccccgg
    13681 ccagcacgcg ggcaacgcct tccagactca tcttcggcaa tacttcaaca taagcaaatc
    13741 cttccgccag tcgtttcgcc cgctcttcct catgcggcgc gccccacgga agtttaatcc
    13801 gtattcctga atcagccagt aaaccaatca attctcgcca gtgttcttcc ggccagtgtt
    13861 tatcatcacg ggtcgtcgca tgaagaaata cggcatattc gccagcatct gtaggcaggt
    13921 tcgtcaggaa atgctgtgca atagcataat cgccctgggt ttgcggtttg ctatagccca
    13981 gacttttggc aaacagttcg cgggtgcgtt ctacggcgtg ctgctgtttt gcaatatgat
    14041 gcttacgatt gtaaaacaga ctggctaaag gttcgcgagc ggtttgccag tccatgccat
    14101 gctttacgcc atgcgccaga cgcgtcacca gcgccgcgct ttttaccagc ccctgagcgt
    14161 cgataactgc gtcatagttc tctgcttgta gcgcttcacg aaacgctttg cgttccgctt
    14221 ttatgggggc cgagaaccag gctttacgcc agcgacgtat tgccacagga ataactcgct
    14281 caacggcggc gtgccaggaa ggaatctgtg cgaacccttc ttccaccacc cagtcaaact
    14341 taatccctgg gattgcctgc tgggcatcag tgagtgcggg caacgtatgg agaacatcgc
    14401 ccatcgacga tgttttaacg atcaaaaccc gcatccgtca ggcttcctct tgtaacaata
    14461 gcgcgttgag ttcttccagt acgcgctggg gagtaatgtc gattaagctc tggtgataac
    14521 cctccgcagc gtcaccttta cgcactttgt gatagccggt aatcaggcgg atcacgcgcg
    14581 ctttatggga tagcggcggt gtgaagtccg ggctactcgg accatacagg gcaaccagcg
    14641 gacgattgag cgccgccgca acgtgcatca ggccagaatc gttagtgaca atggctttac
    14701 aggctgcaat caggataacc gcttgatcaa gctgtgtttc ccccgccagg ttccgacacc
    14761 atgcctgctg ctcggtattc aaagcggcaa gaatctcatt gcccgcttca tgatctttcg
    14821 ccgagccaaa cagaaccacc tgataacctt catcaatcag ctgctttgcc agttccgcat
    14881 agtggtagtg tggccagcgt tttgccggac caaactccgc acccgggcaa aaaccaatca
    14941 tcggacgttc tgatgaaagc gaaaattgat tacaggtata tgatttttca ccttcgctca
    15001 cctgcaattg cggccataac aacggttttg gtagatcttg tgcggtacgc atcacgcctt
    15061 tgtcataggc cagcgcgaca tagcgttcca ccattagcgg ccaggcttct ttatcgagca
    15121 cgcgtaaatc gttgagtaaa ccgtagcgca tctcgccgcg ccagccggtg cgatgaggaa
    15181 tacccgcgaa gaaaggcact aatgcagatt tgaaggagtt gggtaagacg taggcgcggt
    15241 cgtagcgctt ttcacgcagg ctatgaccca gtttgcggcg ttcgccgatt tccagcgctc
    15301 cgtgaccgag aggcatagga atagcttcgt taacttccgg catccgcgat aataatggac
    15361 ggcaccatgc cggtgccatc acatcgatta tcgcctgggg atagcgcgcc tggagcgtgc
    15421 gatagagact ttgcgacatc atcatgtcgc caacccaaga cgggccgatc accagtattt
    15481 tcatgcagag ctcttatgcg tcgcgattca gccaggccat gtattccgtt acaccttcag
    15541 caacggtttt gaacggtttg tcgtaacccg ccgcgcgcag atttgtcaga tctgcctgag
    15601 tgaacgcctg gtagcggcct ttcagcttat ccgggaacgg aatgtattcg atctggcctt
    15661 tcttgtgata agccagcgta gcatcagcta cagcctggaa ggattccgca cgaccagtac
    15721 cgaggttgaa gatgccggaa acgccatttt ccaggaacca cagattcaca tcagccacgt
    15781 cgcccacata gacgaagtcg cgtttgaagt tctcgctacc ttcaaacagc ttcggtgatt
    15841 caccgttgtt aagctgggtg ttgagatgga aagcgacgct cgccatgctg cctttatggc
    15901 cttcacgcgg tccataaacg ttgaaatagc ggaagccaac aatctgcgag ttagcttcag
    15961 gcaggatttg gcgaacatat tcatcaaaca ggaattttga gtaaccgtag acgttcaacg
    16021 gtttttcgta ctcgcgggat tcaataaagt cggaggtgcg tccgccgtag gtggctgcgg
    16081 aagaggcata caggaacggg atttcgcgct ccagacagta gtgcagcagc tctttggagt
    16141 attgatagtt gttatccatc atatacttgc catcccactc ggtggtggaa gagcacgcgc
    16201 cttcgtggaa aatcgcttcg acatcgccga actcttcgcc agccataatc tggatcagga
    16261 agtcttcctt atccatatag tccgcgatat ccagatccac caggttcaca aacttggtgc
    16321 cgtctttcag gttgtccacc accagaatat cggtgatgcc tttatcattc agggccttaa
    16381 cgatgttgct gccgataaag cccgcgccgc cggtaacgat gatcataact gtaaccttcg
    16441 aattatggag tcagagacaa tctcagacac gaatacttct atcatatcac tacacggcgt
    16501 tggcttcagc cattcgccaa aagtgagatt gatggacgtt atttatcctg aattttgcag
    16561 aagtgttaac gcgtcatctc gtcgcgacct ataagtttgg gtaatatgtg ctggaatttg
    16621 ccctgtctgg agaatcgcaa tgcgtggaga attttatcag cagttaacca acgatctgga
    16681 aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt attacgtctg cgcagcaagc
    16741 agatatcact gtggctgatg gaagccacgt cattaacttt tgtgccaata actatctcgg
    16801 gctggcgaat tatccggatc tgattgcggc ggcaaaggcg ggaatggatt ctcacggttt
    16861 cggcatggct tcggtgcgtt ttatttgcgg cactcaggac agccataaag agcttgaaca
    16921 aaaactggcg gcattcctgg ggatggaaga tgcgattctc tactcttcct gctttgatgc
    16981 taacggtggc ctgtttgaaa cgctgctggg cgcggaagac gccattatct ccgacgcact
    17041 gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa gctaaacgct atcgctatgc
    17101 caacaacgat atgcaggagc tggaagcacg tctgaaagaa gcgcgtgaag ccggtgcgcg
    17161 tcatgtgctg attgccaccg atggtgtgtt ctcgatggac ggcgtgattg ccaacctgaa
    17221 gggcgtttgc gatctcgcag ataaatatga tgccctggtg atggtagacg actcccacgc
    17281 ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa tactgcgatg tgatgggccg
    17341 ggtcgatatt atcaccggca cgctcggtaa agcgctgggc ggggcttctg gtggttatac
    17401 cgcggcgcgc aaagaagtgg ttgagtggct gcgccagcgt tctcgtccgt acctgttttc
    17461 caactcgctg gcaccggcca ttgttgccgc atccatcaaa gtactggaga tggtcgaagc
    17521 gggtagcgag ctgcgtgacc gtctgtgggc gaacgcgcgt cagttccgtg agcaaatgtc
    17581 ggcggcgggc tttaccctgg cgggagccga tcacgccatt attccggtca tgcttggtga
    17641 tgcggtagtg gcgcagaaat ttgcccgtga gctgcaaaaa gagggcattt acgtcaccgg
    17701 tttcttctat ccggtcgttc cgaaaggtca ggcgcgtatt cgtacccaga tgtctgcggc
    17761 gcatacccct gagcaaatta cgcgtgcagt agaagcattc acgcgtattg gtaaacaact
    17821 gggcgttatt gcctgaggat gtgagatgaa agcgttatcc aaactgaaag cggaagaggg
    17881 catctggatg accgacgttc ctgtaccgga actcgggcat aacgatctgc tgattaaaat
    17941 ccgtaaaaca gccatctgcg ggactgacgt tcacatctat aactgggatg agtggtcgca
    18001 aaaaaccatc ccggtgccga tggtcgtggg ccatgaatat gtcggtgaag tggtaggtat
    18061 tggtcaggaa gtgaagggct tcaagattgg cgatcgcgtt tctggcgaag gccatatcac
    18121 ctgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat ctgtgccgca acacgatcgg
    18181 cgtcggcgtt aaccgcccgg gctgcttcgc cgaatatctg gtgattccgg cgttcaacgc
    18241 cttcaaaatt cccgacaata tctccgacga cctggcttcc atttttgatc ccttcggtaa
    18301 cgccgtgcat accgcgctgt cgttcgatct ggttggcgag gatgtgctgg tctctggtgc
    18361 aggcccgatt ggtattatgg cagcggcggt ggcgaaacac gttggtgcac gcaatgtggt
    18421 gatcactgat gttaacgaat atcgtctcga actggcgcgc aaaatgggta tcacccgtgc
    18481 ggtcaacgtc gctaaagaaa accttaatga tgtgatggca gaactgggca tgaccgaagg
    18541 ctttgatgtc ggtctggaaa tgtccggtgc gccgccagcg tttcgtacca tgctcgacac
    18601 catgaaccac ggcggtcgca ttgcgatgct gggtattcca ccgtccgata tgtctatcga
    18661 ctggaccaaa gtaatcttta aaggcttgtt cattaaaggt atttacggtc gtgagatgtt
    18721 tgaaacctgg tacaagatgg cggcgctgat tcagtcaggt ctggatctct cgccgatcat
    18781 tacccatcgt ttctctatcg atgatttcca gaagggcttt gacgctatgc gttcgggcca
    18841 gtccgggaaa gtaattctga gctgggatta acacgaacaa gggctggtat tccagccctt
    18901 ttgcctgagg ataatccgtt aaatatgtaa aatcctgtca gtgtaataaa gatatcgtaa
    18961 ttgcgctgat ctcttatata gctgctctca ttatctctct accctgaagt gactctctca
    19021 cctgtaaaaa taatatctca caggcttaat agtttcttaa tacaaagcct gtaaaacgtc
    19081 aggataactt cagaggtcgt cggtaattta tgatgaacag caccaataaa cttagtgtta
    19141 ttattccgtt atataatgcg ggcgatgatt tccgcacttg tatggaatct ttaattacgc
    19201 aaacctggac tgctctggaa atcattatta ttaacgatgg ttcaacggat aattctgttg
    19261 aaatagcaaa gcattacgca gaaaactatc cgcacgttcg tttgttgcat caggcgaatg
    19321 ctggcgcatc ggtggcgcgt aatcgtggga ttgaagtggc aacgggcaaa tatgtcgctt
    19381 ttgtcgatgc tgacgatgaa gtctatccca ccatgtacga aacgctgatg accatggcgt
    19441 tagaggacga cctcgacgtg gcgcagtgca acgctgactg gtgttttcgt gaaacgggag
    19501 aaacctggaa atccatcccc agcgatcgcc ttcgctcaac cggcgtatta accggcccgg
    19561 actggctgcg gatggggctt tcttcgcgcc gttggactca cgttgtctgg atgggggttt
    19621 atcgccgcga tgttattgtt aaaaataaca ttaaatttat tgccggatta catcatcagg
    19681 atattgtctg gacaacagaa tttatgttta atgcgctgcg tgcgcgatat accgagcaat
    19741 cgttatataa atattatctg cataatacgt cagtgagtcg gttgcataga caagggaaca
    19801 aaaaccttaa ttatcaacgt cactatatta agattactcg tctgctagag aaattaaatc
    19861 gaaattatgc cgacaaaatt acgatttatc cggaatttca tcagcaaata acttacgaag
    19921 cattgcgtgt ttgccatgcg gtgcgcaaag agccggatat tattacccgc caacggatga
    19981 ttgccgagat atttacttcc ggtatgtggt aatgactcca acttattgat agtgttttat
    20041 gttcagataa tgcccgatga c
//
LOCUS       DAQSWQ010000073        20041 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.073, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000073 DAQSWQ010000000
VERSION     DAQSWQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.073"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            192..296
                     /locus_tag="R8G18_002796"
     CDS             192..296
                     /locus_tag="R8G18_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HEE9796448.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="R8G18_002797"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="R8G18_002797"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HEE9796449.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="R8G18_002798"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="R8G18_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HEE9796450.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="R8G18_002799"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="R8G18_002799"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HEE9796451.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="R8G18_002800"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="R8G18_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HEE9796452.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="R8G18_002801"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="R8G18_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEE9796453.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="R8G18_002802"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="R8G18_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HEE9796454.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="R8G18_002803"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="R8G18_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HEE9796455.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="R8G18_002804"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="R8G18_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HEE9796456.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="R8G18_002805"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="R8G18_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEE9796457.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /locus_tag="R8G18_002806"
     CDS             complement(9260..9586)
                     /locus_tag="R8G18_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796458.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="R8G18_002807"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="R8G18_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(11620..13284)
                     /gene="glnS"
                     /locus_tag="R8G18_002808"
     CDS             complement(11620..13284)
                     /gene="glnS"
                     /locus_tag="R8G18_002808"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEE9796459.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13543..15489)
                     /gene="nagE"
                     /locus_tag="R8G18_002809"
     CDS             complement(13543..15489)
                     /gene="nagE"
                     /locus_tag="R8G18_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HEE9796460.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            15822..16622
                     /gene="nagB"
                     /locus_tag="R8G18_002810"
     CDS             15822..16622
                     /gene="nagB"
                     /locus_tag="R8G18_002810"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEE9796461.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16682..17830
                     /gene="nagA"
                     /locus_tag="R8G18_002811"
     CDS             16682..17830
                     /gene="nagA"
                     /locus_tag="R8G18_002811"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEE9796462.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            17839..19059
                     /gene="nagC"
                     /locus_tag="R8G18_002812"
                     /gene_synonym="nagR"
     CDS             17839..19059
                     /gene="nagC"
                     /locus_tag="R8G18_002812"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HEE9796463.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            19107..19859
                     /gene="nagD"
                     /locus_tag="R8G18_002813"
     CDS             19107..19859
                     /gene="nagD"
                     /locus_tag="R8G18_002813"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HEE9796464.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         4910 a         5065 c         5033 g         5033 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtgaaat tccagcaaga tgaaagcggt tacattttat ttaattatgt tttgttattt
    11341 taaatttatt ttatggataa attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa
    11401 acgccgctga acgcggcgtt ttggattgtt tgcatatttc ttgccggatg cggcgtgaac
    11461 gccttaaccg gcctacaaaa gcacgcaaac tcaatatatt gcagagatca tgtaggcctg
    11521 ataagcgtag tgcatcaggc aatttagcgt ttgaaactgt ttgtctgttc cttgccggat
    11581 gcgacgtaaa cgccccatcc ggcatagcga aacttaaaat tactcgccta ctttcgccca
    11641 ggtatcacgc agcccaacgg tgcggttaaa taccggtttt tccgccgtag aatggcggct
    11701 atcgaggcag aagtaacctt cacgctcaaa ctggaatgct ttacccgcaa ccgcatcttt
    11761 cagcgacggt tcagcaaagc cctgtttgat caccagcgat tccgggttaa tcaccgacag
    11821 gaaatcatcc gcagcaccag ggttcggcac gctgaacaga cggtcgtaca gacggatttc
    11881 aaccggcagc gcatgtgccg cgctcaccca gtgaataaca cctttgactt tacgaccatc
    11941 tgccggatct ttgcttaagg tgtcggcgtc ataagtacag aagatggtgg tgatattgcc
    12001 ttcggcatct ttctccacgc gttctgcctt aatgacgtaa gcattacgca gacgcacttc
    12061 tttacccagc accagacgtt tgtactgctt gttagcttct tcgcggaaat cggcgcgatc
    12121 gatccaaatc tcaccgctaa acggcacctg gcggctaccc atttccggtt tgttcggatg
    12181 gttcggcatg gtaaccattt cgccttcgcc ctgatagttt tcgataacca gtttcaccgg
    12241 atcgataacc gccattgcgc gcggggcgtt ttcgttgaga tcttcacgga tgcaggattc
    12301 cagcgacgcc atctcaatgg tgttgtcctg cttggtcacg ccgatgcgtt tgcagaactc
    12361 acgaatagaa gcagcagtgt aaccacgacg acgcagaccg gaaatggtcg gcatacgcgg
    12421 gtcatcccag ccttcaacgt gcttgtcggt caccagcagg ttcagcttac gcttggacat
    12481 cacagtgtat tccagattca ggcgcgagaa ttcgtactgg cgcgggtgaa caggaatggt
    12541 gatgttgtcc agcacccagt cgtacaggcg acggttgtcc tggaactcaa gcgtacacag
    12601 agagtgcgta ataccttcca gggcatcgct gatgcagtgg gtgaagtcgt acatcgggta
    12661 gatgcaccac ttgttgccag tctggtggtg ttcagcaaat ttaatgcggt acagcaccgg
    12721 atcgcgcatc acgataaacg gtgaagccat gtcgattttc gcacgcaggc aggctttacc
    12781 ttcttcaaaa ccaccggcac gcattttttc gaacagcgcc aggttctctt caacgctgcg
    12841 gtcgcggtac gggctgtttt tacccggttg cgtcagggtg ccgcggtatt cgcggatctg
    12901 ttccggcgtc agttcatcaa cgtacgccag gcctttattg atcagttcga tcgcataggc
    12961 gtggagctga tcaaaataat cggaggagta acggacgtta ccagaccagt gaaaacctaa
    13021 ccactctacg tcgtttttga tcgactcaac atactcgata tcttctttta ccgggttagt
    13081 gtcgtcgaaa cgcaggttgc actggccttt atagtcctgg gcgatcccga agttcaggca
    13141 gatagatttc gcatggccaa tatgcagata gccattcggc tccggcggga aacgggtgtg
    13201 tactgtggtg tgcttaccac tggccagatc ttcatcgatg atctgacgga taaagttagt
    13261 cgggcgggct tctgcctcac tcatcgtgga ttcctcaaag cgtaaacaac gtataacggc
    13321 gtatgatctt ataagcggga caggctgaca actgttagtt agcgaaaatc gaaaatctca
    13381 ctgagattat ggggatgatt gatgaaaaaa aaagcggcag agaagatctc caccgctttt
    13441 gccagtcccg cgtcattgtt ggatgcgcgt cttatccaac caggactgag cttttgtagg
    13501 cctgataaga cgcgtcaagc gtcgcatcag gcataaagca gattactttt tgatttcata
    13561 cagcggtgtt tgacccgcca caacattgcc ctgagcttta atgatcaagc cactgaaatc
    13621 gtcgatattg ctgcaaacca ccgggctaat catcgaacgg gcgttagcgt tcaggtaatc
    13681 cagatccatt tccagaatcg gttgccctgc gctcacctgc gcgccctctt ccaccagacg
    13741 tttaaagcct ttaccttcca gcgctacggt gtcgataccc atgtggacga caatctccgc
    13801 gcctttttcg gtttctaggc agaacgcgtg gttggtgttg aagattttca cgattgtccc
    13861 tgcggctggt gatacgacga ttttatctgt cggtttcacc gccacaccgt cacccaccgc
    13921 tttgctggcg aatgcttcgt cagaaacctg atccagtgcc acgacatcac cggtaatcgg
    13981 cgataccagc tccgcgatag ataccgcgtt tggtacagcc tgcggttttg ctacaggagc
    14041 ggcagttgcc ggagttgctt cagctgacgc agctgctacc gggccacggg caacgacttt
    14101 cttcatcgca tcgccaatgg attctgcttt cgcgccaaca atcacctgaa tggtctgttt
    14161 gttcagtttt actacaccag aagcacccag acgtttacac atcgcatcgt tgacgcgagc
    14221 tgagtcagcc acggtaaggc gcagacgggt aatacaggcg tcaatcgctt tcaggttgtc
    14281 agtaccgcca accgcagcaa tatagttggt tgccagttga gtcagacctt cttcagtatt
    14341 gctgttagcc tcttcagtta cgatctcgtc ttctttatct tcacgacccg gcgttttcag
    14401 gttgaacatg cggataacca aactgaacac cacgaagtag atagcgaaga agacaacacc
    14461 catcaccagc agcatccaga cgttctggct ggcggccggc aggttataca tcaacgcgta
    14521 gtcgatagcc cccgcagaga aagagaagcc agcatggata cccagcagcg ttgccacaaa
    14581 caggctgata ccggtcagca gtgcgtgcag gaggtacagc agcggagcaa ggaacatgaa
    14641 caggaattcc agcggctcag tcacaccggt caggaacgca gtaacagcaa cagaaagcag
    14701 catcccgcca accatcggac gacgctcttt cggtgctgcg aagtacatcg ccagcgccgc
    14761 acccggcaga ccgaacatca tgatcgggaa gaagccggac atgaacatcc ccgcggtgcc
    14821 gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc gtacccgccg cgttggtgaa
    14881 ttcaccaatc tggaaccagg cgatggtgtt cagcacctga tgcagaccgg ttgggatcag
    14941 cagacggttg atgaaaccaa agataccgga acccagcgcg cctgcagaaa cgatccactc
    15001 gccgcctgca tggatagcgt gctgtactgg cggccagacg taaccaaaaa tggccgccag
    15061 caccaggcag aagaagccgg tggcaatcgg cacaaagcgt ttgccgccga agaagctcag
    15121 gaagtccggc agtttaatat cggaccaacg gttataggct gcgccaccaa ccagaccggt
    15181 aatgataccc gccagtacac ccatgttaat ttctgggttg atggtcacca tcgctttggt
    15241 taacacaaag taacctaccg cacccgccag cgccgccgca cctgcgctgt ctttcgacca
    15301 gctggatgcc acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg caccgcccgc
    15361 ctgggcaata aacgcaacgt taagtaaatc tggctgaccg aatcgcagca acagtgccgc
    15421 caccggcagc accgcgatag ggagctgtaa cgccctaccg agtcgctgga aaaaacctaa
    15481 aatattcatc ttattccccc tacgagaacc ctatttggct cgtttaaagc cgtattttta
    15541 ttttgctgca aattgtactg ccgatgttct gtaatcagat tgttagatca tctgctacag
    15601 agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgcgt gccttttgtg agttttgtca
    15661 ccaaatatcg ttatcatcac tcccttttac tggctaaagc agaaaactta ttttatcatt
    15721 caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc aggttacgct taaagatgcc
    15781 taatccgcca acggcttaca ttttacttat tgaggtgaat aatgagactg atccccctaa
    15841 ctaccgctga acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt atcaatgcgt
    15901 tcaaaccgac tgccgatcgt ccgtttgttc tgggcctgcc gactggcggc acgccgatga
    15961 ccacctataa agcgttagtc gaaatgcata aagcaggcca ggtcagcttt aagcacgttg
    16021 tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga gcatccggaa agctactaca
    16081 gctttatgca ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac atcaaccttc
    16141 tcaacggcaa cgccccggat atcgacgccg aatgccgcca gtatgaagaa aaaatccgtt
    16201 cttacggaaa aattcatctg tttatgggcg gtgtaggtaa cgacggtcat attgcattta
    16261 acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa aaccctgact catgacactc
    16321 gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa tatgccctga
    16381 ctgtcggtgt tggtacattg ctggatgccg aagaagtgat gattctggtg ctgggtagcc
    16441 agaaagcact ggcactgcag gctgcagttg aaggttgcgt gaaccacatg tggaccatca
    16501 gctgtctgca actgcatcca aaagcgatca tggtgtgcga tgaaccttcc accatggagc
    16561 tgaaagttaa gactttaaga tatttcaatg aattagaagc agaaaatatc aaaggtctgt
    16621 aattgttatc cctgccctgg ctccttgctc agggcaatat tttttaaaat cggggggcag
    16681 aatgtatgca ttaacccagg gccggatctt taccggccac gaatttcttg atgaccacgc
    16741 ggttgttatc gctgatggcc tgattaaaag cgtctgtccg gtagcggaac tgccgccaga
    16801 gatcgaacaa cgttcactga acggggccat tctctccccc ggttttatcg atgtgcagtt
    16861 aaacggctgc ggcggcgtac agtttaacga caccgctgaa gcggtcagcg tggaaacgct
    16921 ggaaatcatg cagaaagcca atgagaaatc aggctgtact aactatctgc cgacgcttat
    16981 caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt acctggcaag
    17041 acatccgaat caggcgttag gtctgcatct ggaaggtccg tggctgaatc tggtaaaaaa
    17101 aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg atttcctgtg
    17161 tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg gaaatggttc ctgcggaagt
    17221 catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca acgcgacgtt
    17281 gaaagaagcg aaagccggtt tccgcgcggg gattaccttt gccacccatc tgtacaacgc
    17341 gatgccgtat attaccggtc gtgaaccggg cctggcgggc gcgatcctcg acgaagctga
    17401 catttattgc ggtattatcg ctgatggcct gcatgttgat tacgccaaca ttcgtaacgc
    17461 taaacgcctt aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc cagcaggtgc
    17521 caacattgaa cagttcattt ttgcgggtaa aacaatatac taccgtaacg gactttgtgt
    17581 ggatgagaac ggtacgttaa gcggttcatc cttaaccatg attgaaggcg tgcgtaatct
    17641 ggtcgaacat tgcggtatcg cactggatga agtgctgcgt atggcgacgc tctatccggc
    17701 gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag tagccaacct
    17761 gacctcattc acacctgatt ttaaaatcac caagaccatc gttaacggta acgaggtcgt
    17821 aactcaataa gagaaagtat gacaccaggc ggacaagctc agataggtaa tgttgatctc
    17881 gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg accagtacgg gccaatctcg
    17941 cggattcaga ttgccgagca aagccagctt gcccccgcca gcgtaaccaa aattacgcgt
    18001 cagcttatcg aacgcgggct gatcaaagaa gttgatcagc aggcctccac cgggggccgc
    18061 cgcgctatct ccatcgtcac cgaaacccgc aatttccacg caatcggcgt acggcttggt
    18121 cgtcacgacg ccaccatcac cctgtttgat ctcagcagca aagtgctggc agaagaacat
    18181 tacccgctgc cggaacgtac ccagcaaacg ctggaacatg ccctgttgaa tgccattgct
    18241 cagtttattg atagctacca gcgcaaactg cgcgagctga tcgcgatttc ggtgatcctg
    18301 ccagggcttg ttgacccgga cagcggcaaa attcattaca tgccgcatat tcaggtagaa
    18361 aactgggggc tggtagaagc tctggaagaa cgttttaaag tgacctgttt cgttggtcac
    18421 gatatccgta gtctggcgct ggcggagcac tacttcggtg caagtcagga ttgcgaagac
    18481 tccattctgg tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa cgggcgcatt
    18541 tttattggcc gcaacggtaa cgtcggtgaa attggccata ttcaggtcga accgctgggt
    18601 gaacgctgcc actgcggcaa ctttggctgc ctggaaacta tcgccgccaa cgctgccatt
    18661 gaacaacggg tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc gctggacgac
    18721 tgcaccatca aaactatctg caaagccgcg aacaaaggcg atagtctggc gtcggaagta
    18781 attgagtatg tcggtcgtca tctgggtaaa accatcgcca ttgctatcaa cttatttaat
    18841 ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg ataaagtgct gctccctgct
    18901 attgaaagct gcattaatac ccaggcgctg aaggcgtttc gtactaatct gccggtggta
    18961 cgttctgagc tggatcaccg ctcggcaatc ggcgcttttg cgctggtaaa acgcgccatg
    19021 ctcaacggta ttttgctcca gcatttgctg gaaaattaat gtgcttttat agtggcgctt
    19081 attgttgtca atattctggg tagtccatga ccattaaaaa tgtaatttgc gatatcgacg
    19141 gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc ggaatttttg cacgggatta
    19201 tggataaagg cctgccgctg gtgttgctga ccaactatcc ttcgcagacc gggcaagatc
    19261 tggcgaaccg ctttgccact gccggtgtcg atgtgccgga tagcgtgttt tatacctctg
    19321 cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa gaaagcatat gtggtgggcg
    19381 aaggcgcact gattcatgaa ctgtacaaag ccggtttcac tattaccgat gtgaaccctg
    19441 atttcgtgat tgttggcgaa acgcgttcct acaactggga catgatgcat aaagcggcct
    19501 atttcgtcgc taacggtgca cgctttatcg ccaccaaccc ggacacccac gggcgcggtt
    19561 tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa aatctccggg cgcaaaccgt
    19621 tctatgttgg taagcccagc ccgtggatca tccgcgcagc attaaacaaa atgcaggcgc
    19681 attcggaaga aacggtgatt gtcggcgata acttgcgcac cgacattctg gcgggcttcc
    19741 aggcaggtct ggaaacgatt ctggtgcttt ctggtgtttc atcgctcgac gatatcgaca
    19801 gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc tgaaatcgac gttatctgaa
    19861 taccacccac gtctgcggac gtggttggct ctgatgacaa acgccaaact gcctgatgcg
    19921 ctacgcttat caggcctacg cagctcctgc aatatattga attttcatgc ttttgtaggc
    19981 cggataaggc gttcacgccg catccggcat gaacaaatcg cactttatca gcaatttggc
    20041 c
//
LOCUS       DAQSWQ010000074        19779 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.074, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000074 DAQSWQ010000000
VERSION     DAQSWQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.074"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..193
                     /locus_tag="R8G18_002814"
     CDS             <1..193
                     /locus_tag="R8G18_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEE9796465.1"
                     /translation="RGAFFISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGT
                     NTLLACCCLLRPEEVNSEY"
     gene            183..356
                     /locus_tag="R8G18_002815"
     CDS             183..356
                     /locus_tag="R8G18_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HEE9796466.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(405..1478)
                     /locus_tag="R8G18_002816"
                     /pseudo
     CDS             complement(405..1478)
                     /locus_tag="R8G18_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1550..4281)
                     /gene="torS"
                     /locus_tag="R8G18_002817"
                     /pseudo
     CDS             complement(1550..4281)
                     /gene="torS"
                     /locus_tag="R8G18_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4364..5392
                     /gene="torT"
                     /locus_tag="R8G18_002818"
     CDS             4364..5392
                     /gene="torT"
                     /locus_tag="R8G18_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HEE9796467.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5365..6057)
                     /gene="torR"
                     /locus_tag="R8G18_002819"
     CDS             complement(5365..6057)
                     /gene="torR"
                     /locus_tag="R8G18_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HEE9796468.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6177..7349
                     /gene="torC"
                     /locus_tag="R8G18_002820"
     CDS             6177..7349
                     /gene="torC"
                     /locus_tag="R8G18_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HEE9796469.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7349..9760
                     /gene="torA"
                     /locus_tag="R8G18_002821"
                     /pseudo
     CDS             7349..9760
                     /gene="torA"
                     /locus_tag="R8G18_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(10399..10704)
                     /gene="cbpM"
                     /locus_tag="R8G18_002822"
     CDS             complement(10399..10704)
                     /gene="cbpM"
                     /locus_tag="R8G18_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEE9796470.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10704..11624)
                     /gene="cbpA"
                     /locus_tag="R8G18_002823"
     CDS             complement(10704..11624)
                     /gene="cbpA"
                     /locus_tag="R8G18_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HEE9796471.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11885..13031
                     /locus_tag="R8G18_002824"
                     /pseudo
     CDS             11885..13031
                     /locus_tag="R8G18_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13325..14566
                     /gene="agp"
                     /locus_tag="R8G18_002825"
     CDS             13325..14566
                     /gene="agp"
                     /locus_tag="R8G18_002825"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HEE9796472.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(14604..14831)
                     /locus_tag="R8G18_002826"
     CDS             complement(14604..14831)
                     /locus_tag="R8G18_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HEE9796473.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(14852..15448)
                     /gene="wrbA"
                     /locus_tag="R8G18_002827"
     CDS             complement(14852..15448)
                     /gene="wrbA"
                     /locus_tag="R8G18_002827"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEE9796474.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15821..15994
                     /locus_tag="R8G18_002828"
     CDS             15821..15994
                     /locus_tag="R8G18_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEE9796475.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(16252..17580)
                     /gene="rutG"
                     /locus_tag="R8G18_002829"
     CDS             complement(16252..17580)
                     /gene="rutG"
                     /locus_tag="R8G18_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HEE9796476.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(17601..18095)
                     /gene="rutF"
                     /locus_tag="R8G18_002830"
     CDS             complement(17601..18095)
                     /gene="rutF"
                     /locus_tag="R8G18_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HEE9796477.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(18106..18696)
                     /locus_tag="R8G18_002831"
     CDS             complement(18106..18696)
                     /locus_tag="R8G18_002831"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEE9796478.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(18706..19506)
                     /gene="rutD"
                     /locus_tag="R8G18_002832"
     CDS             complement(18706..19506)
                     /gene="rutD"
                     /locus_tag="R8G18_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HEE9796479.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(19514..>19779)
                     /locus_tag="R8G18_002833"
     CDS             complement(19514..>19779)
                     /locus_tag="R8G18_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HEE9796480.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         4922 a         5054 c         5108 g         4695 t
ORIGIN      
        1 tcgtggagcc ttttttatat ctgccttatt tttcttcaac gctgtatgta tagtaagcga
       61 taacctgttg attattgaat ctttcgggga gatggcttat aacatttctt acctgaccag
      121 ggtaccggga accaacacct tactggcgtg ttgctgtctt ttaagaccag aagaggttaa
      181 cagtgaatat tgaagagtta aaaaaacaag ccgaaacgga aatcgccgac tttatcgcgc
      241 aaaaaatcgc cgagctgaac aagaatacag ggaaagaagt ctctgaaatt cgcttcaccg
      301 cacgagaaaa aatgaccggg cttgaaagtt atgatgtcaa aatcaaaata atgtgatttt
      361 gtgaacatca ccccgtgcga ggtgatgttc cgcttgttgc taatttagtg accaatcatt
      421 ggcgcttgtg gaattaagcg tcggtacaat tcctccggca ccgggctttg ccatactccc
      481 gcatacattg cgtaaccaat caccgcgaac ataatcctca gaaccagtag cgtcattaac
      541 cagccagaca acgcaaaagc ttttttattt gccgcaggtt tttgcagtga aaaggtcaat
      601 gttgaggcta ccggacatga ctctacgcaa gtcatacagc cggtacattc cactgttcgt
      661 acctgaatta atttatctac cgggatccgt gatgggcaat tttttgcgca tttgccacag
      721 ccgatacaac tttcggcatt gcgacgaatc ttaaacggcg ataatagcga aaccacgccc
      781 atcagcgcgc catatgggca aagataacga cactaggcat ggcgaataaa caggctggca
      841 atcagcaaaa cggtcacgct gattaatgtc gcggtcccca tatgacgaaa gaaatcgagc
      901 atttttacgt ccatcaccac gctgtagggc gacaacataa aatagtgaat cgcctgagcg
      961 ggcatcaata acgcgatata gagaaaaaaa ctcaacagca aatacttcac gccgcgcaga
     1021 ggaatatcca gccagcgggg aaggacacat tgccgaccaa acagtttgtt accgagatcg
     1081 ccgattaatt cagaaagcgt accaaccggg cataaccatg agcaaaaggc ctttttgagt
     1141 aatagactga tgacgataaa agcgaccaat aacagcatcg cggcggcgtg gacggacggt
     1201 aactgacctg ttgtaaggct atatttcaga ttcatcagac cggcaatcgg tagccagcct
     1261 tcgatacctc ccggtctggc gacaaatgtc gtgctacttg ccgtttcgta atagcgcacc
     1321 caataccaga acgtgatggc aatataaata ttcattgcca acagtaataa ttgcgtcgct
     1381 ttacgccagg tcgtggcatt gcgccagtca ttccacggta atttgccgcc cgtcgtgcct
     1441 ggccgccgct gccagcgggt tctttttttc tctgccatga ttttgccagt ccgttaagtt
     1501 gcataccaaa tgccactatt ctagttgttc ttaactggct gatattgatt caaatcgcgt
     1561 tcaggtcttt cttatgcaac catgcttcca gagcggcaac actgcgtgta atttcttcgt
     1621 gtggaagggg ggcagataat ggctgctgct ccagttgtgc gcatagctgg ctggcgatac
     1681 gcattcccaa actcgagcaa ctgcttttta gctgatgcgc ggcacgcttt attttctcgc
     1741 tatcctgact ggcgcgggca atgtcgattt catcgagaag cggcagggca tgttgtgtaa
     1801 ataataccag ccattcgtgg atcttctccg tccccattaa ctgagcatct tcattgagtt
     1861 gcgatacatc cagcgattga tcgttattga cttgcagttg gagatagtgc cgagcacttc
     1921 acgcggcacc ggtttaggga taatcccgcg gaataatgaa ctggtacgct ggcgcagcgt
     1981 ttcgtcaatg acatgggcgc taaagccaat caaaaccagc gacggatatt gctgtgccag
     2041 ttgtcgggca agcgtaatgc cgtcgacatc cggcagatca aaatccacca gtgcggcagc
     2101 aaacggttcg ctattttgca gtgtctctaa agcctgcgcg gcattgccaa cagcaacaac
     2161 ctgcgcacca ctggtgttca gcatctcaac ggtaattcgc tgggttagcg ggttatcttc
     2221 aattaacagc aaacgtaaac cgtcaagacg caccgcctga ttgactgttt ttggcacggg
     2281 tgccgtggca acacgtaacg gcaagcgtaa acaaaaacag cttccaacct ccggcgtgct
     2341 ggtggcgctc agttcgccgc ccatcgcctg ggccagacgg ctactgatag tcagccccag
     2401 cccggtgccg ccgcgtttgc cgcttacctg cacaaatggc tggaagattt ctgccagttt
     2461 cgcgggatca ataccgcagc cgctgtcttc cacttcgacc agccattgct cgccatcagt
     2521 gcgactacgc aggataatgt acccttcgtc agtaaaacgc agggcgttgc tcaacaggtt
     2581 ggttataacc tgacgaatac gtcgtggatc gcccattaac gcgcacggca tatcatcggc
     2641 aattgccgtt gccaggcgaa tcgggcgacc tttcacccgt ccgctcatta attgcagggt
     2701 actttccagc agcgggcgcg gttcaaaggg ctcatcgctg accgaaacat tcttgccacc
     2761 tgcttcgata gcggaataat cgagaatatc gttgaggatg gtcagcaacg attcaccaga
     2821 gtcagtaatt gcccgcaaat catcacgctg ggcgttaagt gcggggttat ctgccagtag
     2881 ctgtgcggtg ccgagaatac cgtacagcgg tgtgcggatc tcatggctca tcgccgccag
     2941 aaacgccgat tttgcctggc tggctttttc tgcttccgcc cgtgcctgtc ggtgttctat
     3001 cactagttcc tgcaattcag ctgtacgcgc tttgacctgc gccgccagct gttcacggtg
     3061 gcgattcagt gcatgaacac tgctgcgaaa cgcacccatc agctgcccga tggtgtccag
     3121 ctcccgtacg ccagcggttt ccgggaaagg ggagtcaata tcaccgtcca gcagccgttg
     3181 cagcgcctgc gtttgttcgg caagtggacg cgtgactgag cgataaacca cgcgccagag
     3241 gatcagaatc agtgcgcaaa gtgaaaccat ccccagcaat aacaggctgt attgcccgcg
     3301 tgcactggct ttttcctgat gcgccagtcc gtgctgatta cgcagctcaa tggtgtcgac
     3361 cagctgactg acttcgctac taaactgcgc gaactgggcg atattatttt gggcgagagt
     3421 ttgcaggtga ttgctgattt cactgtcctg ctggaacagc gccaacagat cgctatattg
     3481 gctaacggta gttaacgttg ttgcgacctg cgcacgaact cccggatctt caatgcgtat
     3541 ttgccgacgt tgcagaattt tcaccgcatt attgagctgc ttttccagcg ttggtgcatt
     3601 tttctggatc tgctccagcc ccagattcat caccatttgc tgcacccgca gagcgctaag
     3661 gcgcagttca ttcatctggt taacatactc aagatcgata tcaatcagcc gatcgagtgc
     3721 actttcagca gcctgacgct gatcttgttc gatcaaatcg taaatcccgg cctgggtcgc
     3781 tccagcggaa gttgtcgcat tattcgcctg accttgcgcc aggcgtgcga tctcatcggc
     3841 ggcagcgact atctgctgac tgagttgctg ttgttgctgg cgtagttgca gacgccgccc
     3901 caccagttcc ccttgctgac gtaacgaacg ggagatctcc tgctcctgtt gttcaatagc
     3961 ggtggtatca aaaccttgtt cccgtaacgc ttgcagcaac gcattaatct tcaggctttg
     4021 tgcggtgagc attcgcccct gcgcctgcca catcttttcg ttatcggcac tggtcaggtt
     4081 ctgcgtggcg aaaagttccc aggcgctggc ttcgctcaac tggcgcgcca tattcatggt
     4141 aggaatcaat gcctgagtat tgtctttttc cacctggctg ataaagcgca ggttgtacca
     4201 tcccaccagg gtactggtca gggttaacag cgccatcagg gcaaagccca tccagagtct
     4261 tcgggtcagg gttaaattca cggtcggtgc actttaggtg aaaaatgttg agtcgcaaag
     4321 cggaatgcat ctagcataaa gccttattat tgatgaggct atcatgcgcg tactgctatt
     4381 tttacttctt tcccttttca tgttgccggc attttcggct gataacctgt tgcgctggca
     4441 tgatgcgcag catttcacgg tgcaagcctc tatgccgctt aaagccaaac gcgcatggaa
     4501 actgtgcgcg ctttatccca gcctgaaaga ttcatactgg ttatcgttga actatggtat
     4561 gcaggaggca gctcgccgtt atggtgtgga tttaaaagtg ctggaggcag gcggctacag
     4621 ccagttggct acccagcaag cacaaatcga ccagtgtaaa cagtggggcg cagaggccat
     4681 tttactcggt agtagcacga cctcatttcc cgatctgcaa aagcaggtag caagtctgcc
     4741 ggtgatcgaa ctggtaaatg ctattgatgc tccccaggtg aaaagccgcg ttggtgtacc
     4801 ctggtttcag atgggctatc aacctgggcg atatttggtg caatgggcgc acggtaaacc
     4861 actgaacgtg ctgttgatgc ccggacccga taatgccggg ggcagtaaag agatggtcga
     4921 gggttttcgc gcagccattg ccggaagccc ggtgcgtatt gttgatattg cgcttggtga
     4981 taacgatatt gaaatccagc gtaacctgtt gcaggagatg ctggaacgcc acccagaaat
     5041 cgacgtcgtt gccggaacgg ccattgcggc agaggcggca atgggtgaag ggcgtaacct
     5101 gaaaacgccg cttaccgtgg tgtcgtttta tctttcacat caggtgtatc gcgggctgaa
     5161 gcggggaaga gtgattatgg ctgccagcga tcaaatggtc tggcaggggg aactggcggt
     5221 tgagcaggcc atcaggcaat tacaggggca atcggtgtct gataatgtca gcccaccgat
     5281 tttagttctg acgccgaaaa atgccgaccg tgaacatatt cgccgctcgc tgtcaccagg
     5341 gggatttcgt ccggtctatt tttatcagca cacatcagcg gctaagaaat aaccttcacc
     5401 atgttgcgtc accagtaaat ccgcgctgag tttatgacgt aaacgacgaa ttaacacatc
     5461 gacggtgcgc aggtcagggt tttccacccg acgcgcagaa agcatacgta gcagacgttc
     5521 acggctgaga atttcgcccg gattcgtcac aaatgccacc aacatttcat actctgcgcg
     5581 ggtcagttta atcggctcgc catcccgctc cagcgtatgg cgcgacacat tcaggcaata
     5641 accggcaaag cgatagcagt tgtcctgagt gtgcggttga gcttgtcgcg cgaggtcgat
     5701 tcgccagagc agatttttca cccgtactac cagttcgcgc agttccagcg gtttggtgac
     5761 gtaatcgtct gcgcccattt ccagcccaac aatacggtca atccgatcgc tgcgtccggt
     5821 aaccagaata atccccaccg ttgagcgttc tcgcagggcg cgggttaaca tcaggccatt
     5881 ttcatcgggt aagttgatat ccagcagaat taaatctacc ggctgattct gcataatttc
     5941 ccgcagcccg gcaccgctcg ctgtaacgga aacggtatac ccctcctgag tgaagtaaga
     6001 ttgtaatcgc gcctgggtaa ccggctcatc ttcaacaata acaatgtgat gtggcatcag
     6061 agggttttac tcattctgtt catattctgc cgtaagccgt tcatcctgac cagtgccgct
     6121 gttcatattt gctcattaag atcgcttcac taaaccataa ttttacaggg gctattatgc
     6181 ggaaactctg gaacgcgcta cgccgaccca gtgctcgttg gtcggtactg gcgctggtcg
     6241 ctattgggat tgtgattggc attgcgctga ttgtattgcc acacgttggg atcaaagtca
     6301 ccagcacaac cgaattttgt gtcagttgcc acagtatgca accggtgtat gaagaatata
     6361 aacagtcggt gcattttcag aacgcctctg gcgtgcgagc tgaatgccat gactgtcata
     6421 tcccgccgga tattccaggc atggtgaagc gcaaactgga agcgagcaac gacatttacc
     6481 agacctttat tgcccactcc attgatacac ctgaaaaatt cgaagccaaa cgcgcggaac
     6541 ttgccgagcg tgaatgggcg cgaatgaaag aaaacaactc ggcaacctgt cgctcctgcc
     6601 ataactacga tgcgatggat catgccaggc agcatcctga agcggcacgt cagatgaagg
     6661 tggcagcgaa agataatcaa tcctgcatcg actgtcataa aggtattgcc caccagttac
     6721 cggatatgag tagcggcttc cgtaagcagt tcgatgagct gcgcgccagt gctaatgaca
     6781 gtggtgacac gctgtactct attgatatta agccaattta tgcggcgaaa ggcgataaag
     6841 aagcctctgg ttctctgctg cctgcttcgg aagtgaaagt ccttaaacgt gacggcgact
     6901 ggctgcaaat tgaaattacc ggctggacgg aaagcgccgg acgtcagcgt gtactcaccc
     6961 aattcccagg taaacgcatc tttgttgcct cgattcgtgg tgatgtgcag cagcaggtga
     7021 aaacgctgga gaaaaccacc gttgccgaca ccaataccga gtggagcaag ttgcaggcca
     7081 ccgcgtggat gaagaaaggc gacatggtga acgatatcaa accgatctgg gcctatgcgg
     7141 attcgttgta caacggcacc tgtaaccagt gccacggcgc accggaaatc gcccactttg
     7201 acgctaacgg ttggatcggc acgctcaacg gcatgattgg ctttaccagc ctcgataaac
     7261 gtgaagaacg caccttgttg aaatatctgc aaatgaatga gtctgacacc gcaggtaagg
     7321 ctcacggcga taagaaggac gaaaaataat gaacaataac gatctctttc aggcatcacg
     7381 tcggcgtttt ctggcacaac tcggcggctt aaccgtcgcc gggatgctgg ggccgtcatt
     7441 gttaacgccg cgccgtgcga ctgcggcgca agcggcgact gaggctgtca tctcgaaaga
     7501 gggcattctt accgggtcgc actggggggc tatccgcgcg acggtgaagg atggtcgctt
     7561 tgtggcggca aaaccgttcg aactggataa atatccgtcg aaaatgattg ccggattgcc
     7621 ggatcacgta cacaacgcgg cgcgtattcg ttatccgatg gtacgcgtgg actggctgcg
     7681 taagcgccat ctcagcgata cctcccagcg cggtgataac cgttttgtgc gcgtgagctg
     7741 ggatgaagcc ctcgacatgt tctatgaaga actggaacgc gtacagaaaa ctcacgggcc
     7801 gagtgccttg ctgaccgcca gtggttggca atcgacgggg atgttccata acgcttcggg
     7861 gatgctggcg aaagctattg ccttgcatgg taatagcgtt ggtacgggcg gagattactc
     7921 taccggtgct gcgcaggtga tcctgccgcg cgtggttggc tcaatggaag tgtatgaaca
     7981 gcaaacctcc tggccgctgg tattgcagaa cagcaaaacc attgtgctgt ggggctctga
     8041 tttgctgaaa aaccagcaag cgaactggtg gtgcccggat cacgatgttt atgaatatta
     8101 cgcgcagcta aaagcgaaag tcgccgccgg tgaaattgag gtcatcagca tcgatccggt
     8161 tgtcacatcc acccatgagt atctggggcg cgagcatgtg aagcacattg cggttaaccc
     8221 gcaaactgat gtgccgctgc aactggcgct ggcgtatacg ctgtacagtg aaaacctgta
     8281 cgacaaaaac ttcctcgcta actactgtgt gggttttgag cagttcctgc cgtacctgct
     8341 gggtgagaaa gacggtcagc cgaaagatgc cgcataggct gaaaaactga ccggcattga
     8401 tgccgaaacc attcgtgggc tggcgcggca gatggcggcg aacagaacgc aaattattgc
     8461 tggctggtgc gtgcagcgta tgcagcacgg tgaacagtgg gcgtggatga ttgtggttct
     8521 ggcggcgatg ctggggcaaa ttggcctgcc aggtggtggc tttggttttg gctggcacta
     8581 taacggcgca ggcacgccgg ggcgtaaagg cgttattctg agtggtttct ccggctctac
     8641 gtcgattccg cctgttcacg acaacagtga ttacaaaggc tacagcagca ccattccgat
     8701 tgcccgtttt atcgatgcga tcctcgaacc ggggaaagtg atcaactgga acggtaaatc
     8761 ggtaaaactg ccgccgctga aaatgtgtat ttttgctgga actaacccat tccatcgcca
     8821 tcagcagatc aaccgcatta ttgaaggctg gcgcaagctg gaaacggtta tcgccataga
     8881 taaccagtgg acctcaacct gccgctttgc cgatatcgtg ctgcctgcga ccacgcagtt
     8941 tgagcgtaac gatctcgacc agtacggcaa ccactccaac cgtggcatta tcgccatgaa
     9001 acaggtggtg ccgccgcagt tcgaggcgcg caacgacttt gatattttcc gcgagctgtg
     9061 ccgtcgcttt aatcgcgaag aagcctttac cgaagggctg gacgaaatgg gctggctgaa
     9121 acgcatctgg caggaaggtg tacaacaagg caaaggacgc ggcgttcatc tgccagcgtt
     9181 tgatgacttc tggaataaca aagagtacgt cgagtttgac catccgcaga tgtttgttcg
     9241 ccaccaggca ttccgcgaag atccggatct cgaaccgctg ggcacgccga gtggcctgat
     9301 tgagatctac tcgaaaacca tcgccgatat gaactacgac gattgtcagg ggcatccgat
     9361 gtggtttgag aaaatcgaac gctcccacgg tgggcccggc tcgcagaagt atccgttgca
     9421 tctgcaatct gtgcatccgg atttccgact tcactcgcag ttatgtgagt cggaaactct
     9481 gcgtcagcaa tatacggtag cgggtaaaga gccagtattc attaacccgc aggatgccag
     9541 cgcgcgcggt attcgtaacg gtgatgtggt acgcgtcttt aacgctcgcg gtcaggtgtt
     9601 ggcaggggca gtggtttctg accgctatgc acccggcgtg gcacgaattc acgaaggggc
     9661 atggtacgat ccagataaag gcggcgagcc tggtgcgctg tgcaaatacg gtaaccccaa
     9721 cgtgttgacc atcgacatcg gtacatcgca gctggcgcag ttgttctccc gtgaactgga
     9781 cgatgaacaa ctgacgcaaa tctccagtgc gcagatggct gaatggtttt cgttgctgaa
     9841 aagcgaaccg ccgctcactg cggcggtgaa tgcgctggaa aaccgtattg ccgctctgac
     9901 agtacgtgac gatgcccgtc tggaactggc cgctgacttt tgcggcctgt ttctgatgac
     9961 cgacaaacaa gcggcgctgc cgtatgcatc ggcctacaaa caggacgagc aagagattaa
    10021 acgcttgtta gttgaggcag ggatggaaac cagtggcaat ttcaacgaat cggcagatca
    10081 tctggcgatc tatctcgagt tgttgagtca tctgcatttt tcgctgggag aggggaccgt
    10141 tcctgcgcga agaatcgaca gtttgcggca aaaaacactg acggcgctgc ggcaatggtt
    10201 accagagttt gctgcgcgtt gtcgacagta tgacagcttt ggtttttacg cggcactaag
    10261 ccagttattg ctggtgttag tggagtgcga ccaccaaaac agataacgtc gtttatgcgc
    10321 ctgaaaagac gcgtttagcg tcgcatcagg cattatggcg cagttgccgg atgcgtcatc
    10381 acatccggca aacacacatc acggatgagc tacaaaccgg gaaagccgct ggcgtagcag
    10441 gcggttttcc tgcttcaggt gcgcaatatc atccattaac gtcagcgcca ccgcgatccc
    10501 cggccagtcc agagccagtt catgacgcag gcgtaccgcg cgttgcacca caatggcggc
    10561 atggtcgtca aatacccagg ttgtttcctg gatctcacgc ggttcaacca cccccaaacc
    10621 gacaatttca ttcaactcct cttcagagat gccggtatgc aggcaaaatt cggtaatagt
    10681 aaaagtcacc gtaacattag ccatcatgct ttcccccaat ctttacgtgg atcaaaagac
    10741 gactgggcgt ctgccagttg ctgccacagc gcggcagtgt tttcatccgg tttcggcggc
    10801 atcacgattt tcagtaccgc atacagatcg ccggtctgtt ttttgctcac cagacctttg
    10861 cctttaacgc gcaatcgttg cccggcctgg ctgcctggcg ggatagtcag caaaatgctt
    10921 tctttcagtg ttggaacggt gactttagca cccagcgccg cttcccacgg gctaaccggc
    10981 accacaattt ccagatcctg gccgacaata tcgaacagcg gatgtggcgc aatatgaatc
    11041 accagccaca aatcgccatt tggaccaccg ttttcgcccg gcgtcccctg gcctttcaga
    11101 cggatgcgtt gaccattgcc gacgcccgcc gggatcttca cattcagcgt tttcgggatt
    11161 tcctgttcga tcatgccaaa ggcgttataa accggcaggt tatagctgat ggtacgctta
    11221 tgctcagtca gcgtttcttc gaggaatacc gccacttcga tttcaatatc gtggccgcgt
    11281 gcggcggggc gttgacggct ctggcgggca tgctgaccga aaattgacga gaagatatcg
    11341 tcaaaatctt cggcattaaa actctgaccg tcgccatgat ggaactgatg gttaaattgc
    11401 ggatcgttgc gatgttgcca catctgatca tactcagcgc gacgttgttc atcacttaac
    11461 acttcccagg cttcagcgac ctctttgaag cgggcttcgg catccggttc tttgctgaca
    11521 tcaggatggt atttacgggc cagtcgacga taggcggtct tgattgtctt gagatcgtcc
    11581 gtcggtttca cgcccatgat ggcgtaataa tcctttaatt ccatagcgtt atctcgcgta
    11641 aatcaacaca aattgaagga acccctgtaa ggtaactcct ataagtgtag ggtaatcctc
    11701 aaaatttcat atgccaacac agaatatgtt attgaaatca tcgcggagag gaggtcgcca
    11761 tcaagatggg ttgctgaaca tattttaaac aggtgaaaaa gggtgagcga tttttgatag
    11821 ttgaaccagg cactttaagt ttaactaggg cgtcattatt tattaaattt tatagacgct
    11881 atatatgggt agtaatatac atggaattag ttgcactgca aataattatt tgaaacaggc
    11941 ctggaacgat ataaaaaatg agtacgaaaa aaatcaaaca tattcaatca cgctttttga
    12001 aaacacactg gtgtgtttta tgcggttata caatgaactc agacgtaaag taaatgaaga
    12061 ggatactcca tgtctggaat gtgaatcact agaaaaagaa tttgaggaaa tgcagaatga
    12121 taatgatcta tcattattta tgagaacatt gcgtactaat gatacacaaa tttattcagg
    12181 ggtttcagga ggtattacat atactataca atatgttcaa aatgttgata ttgttagagt
    12241 gtccttgccg ggcagaggtt cagagtctat cacagatttt aaaggttatt attggtatgg
    12301 ctttatggaa tatattgaaa acattactgc gtgtgatgat attttttctg agtattgttt
    12361 agatgatgaa aatatgagta tccagccaga acagataaat acgccgggaa tatctgattt
    12421 agatactggt tttaaaagaa catgagtatt catgggcgct gggcggccat aagacgcaaa
    12481 agcaagtata cgaagagttt attaaaattg ttgataaaca aacgatcgag gctcttgttg
    12541 atacggcatt ctataatctc agggaagatg taaagacgtt atttggcgtt aatctacaat
    12601 ctgacaacaa aattcagggg cagggtggtt ttttatcatg gagttttttg tcttttaaaa
    12661 aagaatttat tgatagttgc ttgaatgaca aaaaatgcat actgcattta cccgaattta
    12721 tatttaatga taacaaggca cgccttgttt tagacaccga cccagagcag aaggttaatg
    12781 aggtgaaaaa tttcctcact gctctttcag atagcatttg ctcattattt attgtttaca
    12841 gcaatgtggc atcaatctcc ttagggaatg aatccttttc aacagatgac gaccttgagt
    12901 atgggtattt aataaacact ggcaatcatt atgacgttta cctccctcct gaactttttg
    12961 ctcaggctta cgagttaaac aataaggaaa ggaatgcgca aattgatttt ttaactcgtt
    13021 atgcaattta atgacaaagg catgtgctaa aaaccattgt tattagcctc acttctctta
    13081 ttattggtac tattgcctct gtattagtaa cgccgctgat attctgttta gccacaggtg
    13141 caattatcag cggcgtacgc gaggcagggg ctaatcaggc atagtttgcg tcaaaccttg
    13201 cctgtttttg aagatatata tagaaaaaca ggcgttcaac aagccatttt gcgaacctgt
    13261 tcccggaaaa aagtcatatt tctgtcacac tctttaatga ttgataacaa aagaggtgcc
    13321 aggaatgaac aaaacgctaa tcgccgcaac tgtggcaggg atagttttac tcgcttcaaa
    13381 tgctcaggca caaaccgtac cggaaggcta tcagctacag caagtgctca tgatgagccg
    13441 ccataactta cgtgcgccgc tggcgaacaa tggcagtgtg ctggagcagt cgacgccgaa
    13501 taaatggcca gaatgggacg tccccggtgg gcaactcacc accaaaggtg gcgtgctcga
    13561 agtgtatatg ggccattaca tgcgtgaatg gctggcagag caggggatgg tgaaatcggg
    13621 ggaatgcccg ccgccggaca ccgtttatgc ctatgccaat agtctgcaac gtaccgttgc
    13681 gaccgcacag ttctttatta ccggcgcatt cccggggtgt gatattcctg tgcatcacca
    13741 ggaaaaaatg ggcactatgg acccaacctt taacccggtg atcaccgatg attccgccgc
    13801 attcagtgaa caggcggtgg cggcaatgga gaaagagctc agcaaactcc agcttaccga
    13861 cagctaccag ctactggaaa aaatcgttaa ctataaagat tcccctgcct gtaaagagaa
    13921 acaacagtgt tcgctggtgg atggcaaaaa tacctttagc gccaagtatc aacaagaacc
    13981 aggtgtttcc gggccgctga aagtcggcaa ctcgctggta gatgcgttta ctttgcaata
    14041 ttacgaaggt tttccgatgg atcaggtggc ctggggagaa atcaaatctg accagcagtg
    14101 gaaggtgttg tcgaagctta aaaacggcta ccaggacagc ctgtttacct caccggaagt
    14161 ggcgcgcaat gttgcgaaac cgctggtcag ttatatcgac aaagctctgg tcaccgatcg
    14221 caccagcgca ccgaaaatta cagtgttggt tgggcacgac tccaacattg cctctctgtt
    14281 aacggcgctg gatttcaaac cgtatcagtt gcatgaccag aacgaacgca cgccgattgg
    14341 cggcaaaatc gttttccagc gttggcatga cagcaaagcc aatcgcgatt tgatgaaaat
    14401 tgaatatgtg tatcagagtg cggaacagtt acgtaatgcc gatgcgttaa ccctgcaggc
    14461 acctgctcag cgtgtgacgc tggaattaag cggttgcccg atagatgcca acggtttctg
    14521 cccgatggat aaatttgata gcgtgttgaa tgaagcggtg aaataacaga aaactccccc
    14581 gcgagaagcg ggggagtcgc tggttaaacg tttttacgtt cgatggtctg ttcgccccaa
    14641 aaaagcgaat ctttatcggc cttagcaaag gctttgacta acacttcatc gctaccttct
    14701 tcccaaatct tttctgccat tttttcgtcg tacccggcga cttcgaaaat ggcctcggct
    14761 atttccggcg acgtattgcg cagagatgcc cattcaccga cgtgatgagc tttcgcttct
    14821 tgagttggca tgcgtatcct cctgttgaag attagccgtt aagtttaact gccagacctg
    14881 cgacatattc cccttgataa cgagcaatag acagttcttc ctggcttggc tggcgtgagc
    14941 cgtcaccgcc tgcgatggtg gttgcgccgt acggcgtacc gccgcgaacc tgtgaaacgt
    15001 caaataattc ctgcgctgcg tagccaatgg ggacaatcac catgccgtga tgcgcaaggg
    15061 tcgtccaggt tgaagtaata gtttgttcct gaccgccgcc agtaccggtg gaactaaaga
    15121 cgctcgccag ttttccgtat agtgcgccgg aagcccacag gccgcccgtc tggtcgagga
    15181 aggtacgcat ttgaccggac atgttgccaa agcgggtagg tgtaccaaaa ataatggcgt
    15241 cgtaatcggc cagttcttgc ggggttgcaa ccggtgcagt ttgcgtttta ccgcctgctt
    15301 tttcaaataa ttgcggcggc atggtttccg gtacacgctt aacgacaact tctgcgccat
    15361 cgactttgct tgcaccctca gcgactgcgc gtgccatcgt ttcaatatgt ccgtacatgg
    15421 aataataaag caccagaact ttagccattt ctaaccactc ctcgtgttat ctctattccg
    15481 tagcgattcg ctaccactta tttaaagata agacgtcctt ttcagagtgc aaatttcaca
    15541 accacttatt tgatttataa caactttcac aagcacgtaa ttttgtcgca aaatgacaca
    15601 tttttatctc atcgcgtttt tttaatcata agagcggctt atggataatt attggagatg
    15661 atatctattc tcgctaagaa gctgttgcag gatattacca aacgcgggtc tacccgcgtc
    15721 agttcactaa gcttagtccc acgtagcgaa aatatggcag ccgccatacg ccgcgttaat
    15781 tctatgcaat atgatgtcta tacccagacg gaggtcagta atggcaaacc atcgaggcgg
    15841 ttccggcaat tttgcagaag accgcgaaag agcatcagaa gcaggtaaaa aaggtggaca
    15901 gcacagcggg ggtaatttca agaatgaccc gcagcgcgca tctgaagcag gtaaaaaagg
    15961 tggcaagagc agtcacggca aaagcgacaa ctagccgggc taatcaatga cgaatgcatt
    16021 tttgtctgta gctcgtcaaa aagccatcac cgccggttac ccggtggttg atactgatga
    16081 caaatgtaag cttgcctgat gcgcgatgct tatcaggcct accagaagat tgcaatatat
    16141 tgaatttgca ctgttttgta ggccggataa ggcgtttacg ccgcatccgg catgaacaat
    16201 gcgtacgttg tcaacaatct gcaccgccgg gtaaccccgg cggttttctg tttatggctc
    16261 ctgatgaaca acttctggcg gtggaacgtc aaccaacctt ctacttaaca acgcattgag
    16321 taaaatcgcg ccaaaggtgg ctgtaccgat ccctcccaac gtaaaaccgc ccagcgtgag
    16381 agcaaaatca cccgcgccca gcactaaggt tactgcgacc ataatcaaat taccgttctg
    16441 gcttaaatcg acacggtttt gtacccagat ccttgcgcct gcgacggcaa tcagcccgaa
    16501 cacaacaatt gatgcaccac caatcaccgc ggccggaatg gtatggatca gcgcgccaaa
    16561 tttcggcgaa aagcccaaca gcatggcgat gacggcagca gcaacaaaca ccagcgtcga
    16621 gtagactttt gtcacggcca tcacaccgat attttcagca taggtggtga cgccgctacc
    16681 gccgacagag ccggaaagca tcgttgccag accatcaccg acgaatgctc gccccatata
    16741 cgggtccata ttgcgtccgg tcatcccggc gaccgccttg agatgaccta agttttccgc
    16801 caccagaatc accgccacgg gcgcaatcag catcattgcc tgactattaa aagcaggagt
    16861 ggaaaaatgt ggcaaaccga accaggcagc atggctgacg agagtaaaat cgacggcttt
    16921 tcccagccct aaaacgttgg tcatcacgcc atacagcaga caggcgacaa ttaaacctac
    16981 gagaatcaat aaccgctgga tcatgccacg ggtaaacacc gccaccagcc caatacacag
    17041 caccgtcatt accgccatcc agctatcaaa ggccgaagcc gatacacttt tcactgcgat
    17101 aggcgctaag ttcaggccaa tcgccatcac caccgcaccc gtcaccaccg gcggcattag
    17161 tcgttcaatc cagcgtgtac caattttcat caccaccagg ccaatgacgg tataaaccag
    17221 cccacaggcg ataatcccgc ccagtgcgat gctgatattc gggttaatgc cctgaccgtt
    17281 aaagcccgtc gcggcaatca ccacgccgac aaaagccgcg ctggagccga gataactggg
    17341 gacgcgcccg ccggtaataa agaaaaacag taacgtgccg atccccgaca ttaaaatgga
    17401 aagattggga tccagcccca tcagaatcgg cattaacacc gtcgcgccaa acatcgccac
    17461 cgcgtgttga acgcccatta ctgccgtctg agcaaacggc aatcgttcat ccggcgcgac
    17521 cacgccgctc tctgtagagg tcgattttaa ctgccagtga ggaaaaccga acattgccat
    17581 cagctgtctc cttaaggagg ttaacaagca gggcgcatca gcgcgtgata actgcgatcg
    17641 aaccacacca gcccgtaggg tgtggtgtga cgatgaatcg cttcgatggc gcaaaacaga
    17701 atgtcgtggg tgccgacgct caccacctgg ctgatgcggc agtcaaacga aaccagagcc
    17761 tcttccagtt gcgggcatcc ggtcacaccc gtctgccagc gggcggcggc aaagcggtgt
    17821 tccatgggcg ttttgccgcc aaaaagattt gaaagcggct cctgcccggc gctcagtgta
    17881 tttacacaca gcgttcgatt ttcattgaat accggccaga cggacgcccc acgattcagg
    17941 cacaccagta atgtgggcgg cgtgtcggtc acgctgcaga cggcgctggc ggtgaacccg
    18001 gcgcgcccgg atggaccgtc cgtggtgatg atattgaccg ccgcgcccat acaggacatc
    18061 gcatcgcgaa aggtttgttg atcaactatg ttcatagttt gctccttaca acagcccgca
    18121 ggcttcttca aaggacagac gtggcaggcg cgcaaaaagt ttgctgctat cgccatagcc
    18181 gatattaatc agcagattgc tcttcagcgt gctgcccgca aaaaaggcgt cgtcaacgtg
    18241 ttgacggtca aaacccgaca tcgggccggt atccagtccc agcgcacggc aagcgacgat
    18301 cagataggcc gcctgcatgg agctgttgcg aaacgctgtt tcttcggcaa gttgtgggct
    18361 ggaggtaaac caactgcggg catcaccgtg gggaaacagt agtggtaacc gttcataaaa
    18421 ttcactgtcc caggcaacga tagcggtgac gggcgcggtc agggtttttt gtagattgcc
    18481 gctggaaagc gccgggtgca gacgttcttt tccttctaca gtgcggataa acacgatccg
    18541 tgccggagaa cagttagctg atgtcggccc ccattttatc ggggcataaa gctcccgtaa
    18601 cgtctcatcg ctgacgggtg tctcccgcca gccgttgtga gtgcgggcat cggtgaacag
    18661 ggtgctaagc gcacctgggc taacggcttc gttcatagca attccttaca gggcggcttc
    18721 acggtgatgt aacaggctgg caagcccgtt gagtaacaga gcattaaacg tttcggggtc
    18781 ggtcacgttg caggcgtgtc cgccatagcg catcaccatt ttctggctat cgggcagggc
    18841 ggcatgaagc tcactggaac atgctgatgg caccagcaga tcatcactgg cgcagatgat
    18901 ttgcaccggg cagcggatgc gatccgcatg gtgactaaag tcagcgcgtt tgagggcgtt
    18961 aagtcgacgc agtaaattat ttttgccctg aaaatgcgcc agtgccagcg cgtcttctgc
    19021 ctcaaggcaa ggtgcgcggg ccgccatcca gtcggcggga tagaggaaca acggctgcgc
    19081 ttccacccat gcctgcgcgc cgccgctata cagtaatcgt tcgcgaacct gaaaacagcg
    19141 gcgcgtatgg gcgtttattc gtagccagcc gttaacgctg atcagcacag ttaccgacgc
    19201 gggataatcc agcgccagct gcattcccac cagcgcaccg agcgcatggc cgaccaccgc
    19261 gtaatgctca atccctgcgg ctaccagcgc ctgatgcagt tccgctgcca tctgggcgat
    19321 actgtaatct tctgccagcg tgtcgggatt attgccggtg ccgcgctggt cgtaacagac
    19381 tacctgatac tcctgctcca gcaccgccag ttgcggtaac cagtaactgc cgctaccgcc
    19441 aagacccgaa atcaacacca ctacgggcgc atcagcataa gggggaggtg agagtgaaag
    19501 tttcatcgcg gcctcacttg gcgatatgcg caattgtggc gatttccacc agcgcgtcag
    19561 gttttaccag tccgcactga atgcagaatc gcgccggttt atcacccgga aaaaactcgg
    19621 cgtagatttc gttaatcgcg gcgtaatttt tccagtcggt aataaagatg ctgttgaagg
    19681 tcacatccgc catcgtgcca cccgccgtct cgatcacctt gcggatagtt tccagaacgt
    19741 ggcgggtttg cgcctttggg tcatcggcaa acagcacgt
//
LOCUS       DAQSWQ010000075        19762 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.075, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000075 DAQSWQ010000000
VERSION     DAQSWQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.075"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..369
                     /locus_tag="R8G18_002834"
     CDS             <1..369
                     /locus_tag="R8G18_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEE9796481.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
     gene            complement(509..704)
                     /locus_tag="R8G18_002835"
                     /pseudo
     CDS             complement(509..704)
                     /locus_tag="R8G18_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            960..1811
                     /gene="hchA"
                     /locus_tag="R8G18_002836"
     CDS             960..1811
                     /gene="hchA"
                     /locus_tag="R8G18_002836"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HEE9796482.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="R8G18_002837"
     CDS             complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="R8G18_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HEE9796483.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="R8G18_002838"
     CDS             complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="R8G18_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HEE9796484.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4081..4494
                     /gene="hiuH"
                     /locus_tag="R8G18_002839"
     CDS             4081..4494
                     /gene="hiuH"
                     /locus_tag="R8G18_002839"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEE9796485.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4603..5607
                     /gene="msrP"
                     /locus_tag="R8G18_002840"
     CDS             4603..5607
                     /gene="msrP"
                     /locus_tag="R8G18_002840"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEE9796486.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            5608..6243
                     /gene="msrQ"
                     /locus_tag="R8G18_002841"
     CDS             5608..6243
                     /gene="msrQ"
                     /locus_tag="R8G18_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEE9796487.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            6500..7150
                     /gene="zinT"
                     /locus_tag="R8G18_002842"
     CDS             6500..7150
                     /gene="zinT"
                     /locus_tag="R8G18_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HEE9796488.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            7493..8023
                     /locus_tag="R8G18_002843"
                     /pseudo
     CDS             7493..8023
                     /locus_tag="R8G18_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(8593..8682)
                     /locus_tag="R8G18_002844"
     tRNA            complement(8593..8682)
                     /locus_tag="R8G18_002844"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8646..8648),aa:Ser,seq:cga)
     gene            8776..9573
                     /gene="mtfA"
                     /locus_tag="R8G18_002845"
     CDS             8776..9573
                     /gene="mtfA"
                     /locus_tag="R8G18_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HEE9796489.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9674..9749
                     /locus_tag="R8G18_002846"
     tRNA            9674..9749
                     /locus_tag="R8G18_002846"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9707..9709,aa:Asn,seq:gtt)
     gene            10063..17127
                     /gene="yeeJ"
                     /locus_tag="R8G18_002847"
                     /pseudo
     CDS             10063..17127
                     /gene="yeeJ"
                     /locus_tag="R8G18_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(17389..18441)
                     /locus_tag="R8G18_002848"
     CDS             complement(17389..18441)
                     /locus_tag="R8G18_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEE9796490.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            18756..>19762
                     /gene="shiA"
                     /locus_tag="R8G18_002849"
     CDS             18756..>19762
                     /gene="shiA"
                     /locus_tag="R8G18_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HEE9796491.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         5513 a         4656 c         4409 g         5184 t
ORIGIN      
        1 gcaatgatgt atgctgaaac ccgcaatatg acaccatatg gtaatgtggg tatcgcaaat
       61 aaaacccaga actttgaagc tgtagcacag tatcagttcg attttggtct gcgcccatct
      121 cttgcatatg tttattcaaa aggcaaagac ctcggcggta acgactataa caacaatggt
      181 catcaagagt atgttgatca ggatctggtc aactatgttg aaattggagc gacctactat
      241 ttcaataaga acttctcaac atatgttgac tataaaataa acttgttgga taaagatgac
      301 gatttctatg acaacaatgg catcgcaaca gatgacgttg ttggtgtggg cttagtctac
      361 cagttctaat cagccaacgt gctgcggcgt gttactgtaa aactaaacac tagcctgagt
      421 tcgtccctca tcgaattcag gcttttttat tggcatttca tctccatctg cggcacacgc
      481 aattgccaat gcatatcgat acagcacctt accctgcccc tcctggcaca ataacatcaa
      541 ccagcgactc ttcaaaccaa cacccaaatg catttcttta tgaattaatc cgtaacaaac
      601 acgcttccca tatagaaata tttgcaaaaa tagtgcatga aataatcttt tctctaccaa
      661 tccgcgatat aaatattcat cagtaccaca atatttcaca gcactaaatc tctccccgcc
      721 gccccgtacc tttgataatg gtctaaaatc attgaggcca cttgcgacga cgttgctcaa
      781 taaggaaaat ttggcgtgaa taatattcaa caccagtgca gcatcatcat tacatactta
      841 cacccgccga ctaccgcttt cgatatattg actcaatttt ccagactggt taaaaatagc
      901 gaccagctca gtcgcaaata tagtgactac cctaactaag caacaataag gaatacacta
      961 tgactgttca aacaagtaaa aatccgcagg tcgatattgc tgaagataat gcattcttcc
     1021 cttcagaata ttcgcttagc caatatacca gtcccgtctc tgatcttgat ggcgtggact
     1081 atccaaaacc gtatcgcggt aagcataaaa ttctggtgat cgccgcggac gaacgttatt
     1141 tgccaaccga taacggaaaa ctgttctcga ccggtaacca tccgattgaa acgttgctgc
     1201 cgttgtatca tctccatgct gctggtttcg aattcgaagt ggcgaccatt tccggtctga
     1261 tgaccaagtt tgaatactgg gctatgccgc acaaagatga aaaagtaatg ccattctttg
     1321 agcagcataa atcgttgttc cgcaatccga agaaactcgc ggatgttgtc gccagcctca
     1381 acgctgatag cgaatatgca gcaatctttg ttcctggtgg tcatggcgca cttattggtt
     1441 taccagaaag ccaggacgta gctgctgctt tgcagtgggc aatcaaaaat gaccgttttg
     1501 ttatctccct ttgccacggc ccggcggctt ttctggcgct tcgccacggc gataacccac
     1561 tgaatggtta ttccatttgc gcattcccag acgccgcaga caaacaaacg ccagagatag
     1621 gctatatgcc gggtcatctc acctggtact tcggcgaaga gctgaagaaa atgggcatga
     1681 acattattaa tgacgacatc accgggcgag tacataagga ccgtaaagtt ctcaccggcg
     1741 acagtccttt cgcagcgaat gcgttgggta aactggcggc gcaggaaatg ctggcagctt
     1801 atgcggatta atccctgtta cgtttgaatg taaacgcata atcaatcgca gccacgttaa
     1861 tactcacgac gtggctgcgt tttttcatgg ttgatttatg gcgtactgaa gccctatgtt
     1921 aatttctttg cggtaacata atccggaaca cattatgctt attgagatag tgatacgtag
     1981 cacttccccc atgtaattcg gcaatcgctt tgactaaaga aagaccaagt ccctgaccta
     2041 cggaatgacg cgaattatct ccccgccaaa atctacggaa gagtttttca ggctcatgaa
     2101 ttttcgttcc aggactggcg acatcaatat taagatagcc gttggtatca agaaaactgg
     2161 ttatatgaat acgcgatttt tctggtgaat atctaatggc attaacaata agattcgata
     2221 acattcgttg cagtaaaatt ttatccgcaa agatttgctg attgcactcg accttaaagc
     2281 aaatctcttt ctcgtctgaa aggtattcaa gataatccaa caaattttcg acttccttat
     2341 tgagagaaag cgagtccagt ttcaccaaaa cattgttttt atctgcccgg gcaagaaaca
     2401 gtatgttctc tgttaaccgc gaaatatttt ccagctcttc aatgttaccg gcaattgttt
     2461 tttgatattc agcgatactt ctggtttgac tgagcgtaac ctgattctga cccagtaatg
     2521 cattaatggg cgttctaagt tcatgagcga gatcgtcagc aaattgactt agacgctcaa
     2581 aatctttgac taaagcatga tgcattttat tcaacgcctg cccaagaggt tttagttctc
     2641 tcggtaatgc gctaacctca acaggctccc ggctatcgtt ataattcagc gcttccgtta
     2701 caccactcaa ctttttgatc tctcgtaatc ccgttctgat taacagcgga cttaatactg
     2761 agcaaagtac aatggcgaca atgcaaatta taatgctatt aattttatac tgttcaagca
     2821 tgttatgtct ggctgaagcc aatttagcca cagtaaccgt taatgatggc gaaacttcat
     2881 caatattaat tcgtaaagca tcaatctcag tatcattaat aatgcttctg taaatgccag
     2941 ctgcgctgat tgtctcacta gcaggtatgt tatttaacat gtcatcactg acatttgtcc
     3001 ggttaacaat tttattgatg ccatcaccat gaatgatcaa gatatcctga ctaacatcca
     3061 tcatccggtt aaagtataca ggtaacgtat ctggatttac cccatcaatt aacaactgct
     3121 tgatctgtgc tgtccggtta atgagtgttg tatcatcgcg ccatttcaac tcacttgcca
     3181 ggccattata gagagtccag acaattccag cgccagcaac agacagcagc aatataaaaa
     3241 gcaaggttaa acggacggtt atagataatc ttttcattat ttttttaccg ctacgaatga
     3301 atagcccatc ccccggattg tggcgattag cttttcagga aaaggatcat caacttttgc
     3361 gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt tgattcccca
     3421 aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg ccagtagcca
     3481 aagtaactga aactccttgc gcgtcagtgt aatactgata ttgttcctgc tcacactttg
     3541 acttacagag tccattctta agccactgat ttctaatgtt gaattcaaag cgtgatgttg
     3601 ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt ttaccagata
     3661 atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc ttgcagtaag
     3721 gcaaataaca ggggtttgct ttgctgttct taacgtttgt aagatctgcc agccatccat
     3781 acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg caagataaag
     3841 cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa gcccctgcgt
     3901 tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga aaataatatg
     3961 ccataaatca tatgttatag aaacagccta gttcattaca aaattgtaat gctgctgtaa
     4021 ggttaccctg gccgcttttt cgctatcctc aaaactcatt cacatgacaa ggatataaac
     4081 atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt accttctttg
     4141 gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca aaccggaaaa
     4201 cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg gttacaactt
     4261 aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga gcaaactgca
     4321 actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa acaaaatctt
     4381 gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa cgagcattat
     4441 catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag ttaatgcata
     4501 aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca gattaacagt
     4561 aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt
     4621 ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta
     4681 aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca tgccgatctg
     4741 cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc gctggagttc
     4801 agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa agtctccggt
     4861 tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa tgctggtagc
     4921 ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc attgaccctc
     4981 gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc
     5041 gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa attgctggcg
     5101 cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa
     5161 caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa
     5221 ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg tgtttatggc
     5281 aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc
     5341 tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc
     5401 tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat
     5461 cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat cctcgatgtt
     5521 cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt
     5581 ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt gacatggctg
     5641 aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat
     5701 cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca
     5761 ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc aaaacagccg
     5821 ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat
     5881 ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt aggcaaggag
     5941 ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta
     6001 gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca acagttgcat
     6061 aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt
     6121 atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc cttacggtat
     6181 aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa attatctgta
     6241 taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc gagcgccctg
     6301 tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc aaattatcaa
     6361 cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta tgctctcatc
     6421 tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat catatacaat
     6481 gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt
     6541 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
     6601 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
     6661 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
     6721 tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg ctgaaattaa
     6781 agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg aggacggcat
     6841 tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
     6901 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
     6961 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
     7021 atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa atgaaatgga
     7081 aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg aggaaatgat
     7141 gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc atcataactg
     7201 ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg ctcatcacta
     7261 tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac ggattccata
     7321 cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata ttggctggtt
     7381 acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct gtataaggaa
     7441 tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa aaatgaaccg
     7501 attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg caataaccta
     7561 tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc tgctgatgcg
     7621 agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac gcctgattat
     7681 aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc aaatgaaagc
     7741 ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc tgctggggat
     7801 tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg tgtctccctt
     7861 tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa cctaggcaaa
     7921 tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc actacattca
     7981 gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc ccctgacaag
     8041 gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca caatgcatcc
     8101 tttctactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt aagacttatc
     8161 aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc
     8221 tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt
     8281 taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct acgcaacttc
     8341 agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg
     8401 agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc
     8461 atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct
     8521 tcaagaatat tctacaattg ttctgtttag gaaaagcaag gcgggaagtc aggagataag
     8581 tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc
     8641 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgccatg
     8701 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     8761 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     8821 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgggtctgac ggaacaggaa
     8881 caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct tgttccttta
     8941 cagggctttg aactggattc attaagaagc tgccggatag cacttctatt ttgcctaccc
     9001 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     9061 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     9121 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     9181 gattcttttg atgcatctgg ttttaacctg attattcatg aagtcgctca taagctggac
     9241 acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga ggttgctggc
     9301 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attggttggc
     9361 gagaatgcgg cgagcattga tgcttatgca gccagcgatc ctgctgaatg ttttgccgta
     9421 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     9481 caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca cgctaatgat
     9541 acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat taattaacca
     9601 attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgtgcc
     9661 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     9721 cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt tagcgcaatg
     9781 tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac accttaagcg
     9841 ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt ttcccctcga
     9901 ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacaccca aaactgccta
     9961 acgaaaagtt attaattttc aatcgtattg ctatcagtat ttacattttt tcgctgtgct
    10021 agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga
    10081 agtggagaag aaataaatga ccgacaaata ttatgcggga tgggaattaa actacgccgc
    10141 ttaactgcgg gtatctgtct gataactcaa cttgcgttcc ctatggctgc ggcagcacaa
    10201 ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat tgcaaatgcc
    10261 aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc
    10321 ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
    10381 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta
    10441 accccgccgc cgggtaatag cagtgacaac ctcgagcaac agatagccag tacttcacag
    10501 caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa tatggcgcgt
    10561 ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg cttcggtacc
    10621 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca gttcgatttt
    10681 ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac tctccatcgt
    10741 actgacgagc gtacgcagat taacaacggc ttaggttggc gtcatttcac tcccacatgg
    10801 atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc ccgcgccggc
    10861 attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta tttgcgactg
    10921 accaactggc gcagcgcacc tgaactggac aacgattata aagcacgccc ggccaatggc
    10981 tgggatgtac gcgcagaaag ctggctaccc gcctggccgc accttggcgg taaactggtc
    11041 tatgaacagt attatggcga tgaagtggcc ctgttcgata aagacgatcg gcaaagtaat
    11101 cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac cttcagcgcg
    11161 gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga ttttacctgg
    11221 caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc acggcgtagc
    11281 cttgcaggca gccgttatga tctggtggat cgcaacaaca atatcgttct ggaatatcgc
    11341 aaaaaagaac tggttcgcct aaccctgaca gaccccgtga cagggaagtc aggagaagtg
    11401 aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa cgtcgaagcc
    11461 accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat tctggttacc
    11521 ctgccggctt accggttcac cagtacgcca gaaaccgata acacctggcc gattgaagtc
    11581 accgccgaag atgtcaaagg caatttgtcg aatcgtgaac agagcatggt ggtcgttcag
    11641 gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac attgaacgcg
    11701 gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg taatcctgtt
    11761 gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct ttctgactgg
    11821 aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat gtctggcacg
    11881 ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc cgtggtgaat
    11941 atcatttctg tttcgtcatc ccgaactcac tcgtcaatta agattgataa ggaccgttat
    12001 ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga caaacctgtt
    12061 aaggaacaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa accaggagtc
    12121 actacagact ggaaagaaac cgcagatggc gtctataagg cgacctatac cgcctatacc
    12181 aaaggcagtg gacttactgc gaagctattg gaatgaagat ttgcataccg ctggatttat
    12241 catcgacgcc aacccgcagt cagcgaaaat tgcgacatta tctgccagca ataatggtgt
    12301 actcgccaat gagaatgcag caaacaccgt ctcggtcaat gtcgctgatg aaggaagcaa
    12361 cccaatcaat gatcataccg tcacgtttgc ggtattaagc ggatcggcaa cttccttcaa
    12421 caatcaaaac accgcaaaaa cggatgttaa tggtctggcg acttttgatc tgaaaagtag
    12481 taagcaggaa gacaacacgg ttgaagtcac ccttgaaaat ggcgtgaaac aaacgttaat
    12541 cgtcagtttt gtcggcgact cgagtaccgc gcaggttgat ctgcagaagt cgaaaaatga
    12601 agtggttgct gacggcaatg acagcgccac aatgaccgcg accgtccggg atgcaaaagg
    12661 caacctgctc aatgacgtca tggtcacttt caatgttaat tcagcagagg cgaaactgag
    12721 ccaaaccgaa gtgaatagcc acgacgggat cgccacagct acgctgacca gtttgaaaaa
    12781 tggtgattat agggttacgg cctctgtgag ctctggttcc caggctaatc aacaggtgaa
    12841 ttttatcggt gatcaaagta ctgctgccct gaccctcagt gtgccttcag gtgatatcac
    12901 cgtcaccaac acagctccgc aacatatgac tgcaaccttg caggataaaa atggcaaccc
    12961 actaaaagat aaagaaatca ccttctctgt gccaaacgac gtcgcaagta agttctcgat
    13021 tagcaacgga ggaaaaggca tgacggatag taacggggtt gcaatcgcct ccctgaccgg
    13081 cacgttagcg ggcacgcata tgatcatggc tcgtctggct aacagcaatg tcagcgatgc
    13141 acagccaatg acgtttgtgg cggataaaga cagagcggtt gtcgttttgc aaacatcgaa
    13201 agcggaaatc attgggaatg gcgtggatga gacaactctg acagcaacag tgaaagatcc
    13261 gtcgaatcat ccggtggcgg ggataacggt aaacttcacc atgccacagg acgttgcggc
    13321 aaactttacc cttgaaaata acggtattgc catcactcag gccaatgggg aagcgcatgt
    13381 cacgctgaaa ggtaaaaaag cgggcacgca tacggttacc gcaacgctgg gtaataacaa
    13441 taccagtgat tcgcagccgg taacatttgt ggcggacaaa gcctcggctc aggttgtcct
    13501 gcagatatca aaagatgaga tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac
    13561 ggttaaagat cagttcgaca atgaggtgaa taatcttccg gtaacattca gctcagcctc
    13621 ttcaggactc accctgaccc cgggagtaag taataccaat gagtctggca tcgcgcaggc
    13681 cactctcgca ggcgttgcct ttggtgagca gacggtcact gcatcactgg ctaataatgg
    13741 tgccagcgac aacaaaactg tgcattttat tggcgacaca gcggcggcaa aaattatcga
    13801 gttgacgcct gtcccagaca gcataatcgc cggtaccccg cagaacagct ccggcagcgc
    13861 catcaccgcc acagtcgttg ataataatgg ctttccggtg aaaggtgtga ctgtgaactt
    13921 caccagcaga acaaactctg ccgaaatgac gaatggcggc caagccgtaa cgaacgaaca
    13981 gggtaaggct accgtcactt ataccaatac ccgctcctcg atagaatcag gagcgagacc
    14041 ggataccgtt gaggccagtc tggaaaatgg tagctccacg cttagcacat caattaatgt
    14101 caacgctgat gcgtctacgg cacatctcac ctggctacag gcactttttg atacagtctc
    14161 cgcaggcgag acaaccagtc tgtatattga ggtgaaggat aattacggca acggtgtccc
    14221 cagcatcagg tgaccctcag cgtttcacca agtgaaggcg tgacccccag taataacgct
    14281 atatatacta ccaaccacga cggcaatttt tacgcaagct ttaccgctac aaaagccggg
    14341 gtttatcaag tgacggcaac cctcgaaaat ggcgattcga tgcaacaaac agtgacctat
    14401 gtgccgaacg tcacgaatgc cgaaatcacg ctggcaacct cgaaggatcc ggtgattgcc
    14461 gacaataacg atctcacgac actaacagca acagtcgctg atacagaggg caatgcgata
    14521 gccaacactg aagtaacatt tactctgccg gaagatgtta aggcgaactt cacgctgagc
    14581 gatggcggta aagcgattac tgatgctgaa ggcaaagcga aagtcacgct gaaaggtaca
    14641 aaagcaggcg ctcatactgt tacagcatcg ataactggcg gtaagagtga gcagttggtg
    14701 gtgaacttta ctgcggatac gctcactgcg caggttaatc ttaacgttac cgaggacaat
    14761 tttatcgcta ataacgtcgg gatgaccagg ctgcaggcaa cagtgactga tggaaacggc
    14821 aacccgttag ccaatgaggc ggtgacattc acgctaccgg cagatgtgag cgcaagcttt
    14881 actctcggac aaggcggttc cgccattact gacatcaacg gcaaggctga agttacactg
    14941 agcggtacaa aatccggcac ctaccccgtg acagttagcg tgaacaatta tggtgtcagt
    15001 gatacgaaac aggtgacttt gattgccgat gctggtaccg caacactagc ctccttaacc
    15061 tctgtatact cattcgtcgt cagcacgacc gagggcgcga ccatgactgc aagcgtcact
    15121 gacgctaacg gcaacccggt agaaggcata aaagttaatt tccgcggaac ctccgtcacg
    15181 ctaagcagca ccagcgttga aacggatgat cggggtttca ctgaaattct tgtgacaagc
    15241 accgaagtcg gactgaaaac agtttcagcc tctctggcag ataaacctac tgaagtcata
    15301 tcgcgattac tgaatgcaaa agcagatatt aattctgcaa cgattaccag tctggagata
    15361 cctgaaggtc aggtcatggt cgcacaagac gtagcagtta aagctcacgt caacgaccag
    15421 tttggcaacc cgattcttaa tgaatctgta acattcagtg cagagccacc agagcacatg
    15481 accatcagcc aaaatattgt ctctactgat acgcatggta tagccgaggt ctccatgacg
    15541 cccgaaagaa acggttcgta tatggtgaaa gcatccctgg cgaatggagc ctcacttgag
    15601 aaacaactgg aggctattga tgaaaaactg acactctcgg cgtccagccc gcttatcggt
    15661 gtcaattccc caacaggtgc aactctgacg gcaacgctaa cttctgcaaa tggcactcca
    15721 gtggagggtc aggtcatcaa ctttagcgta acgccagaag gtgcgacgtt aagtggcgga
    15781 aaagtgagaa ctaactcttc tggtcaggct ccagtcgttc tgaccagcaa taaagtcggt
    15841 acatatacgg tgactgcatc gttccataac ggcgtaacaa tacagacaca gacaaccgtg
    15901 aaagtcactg gcaactcaag caccgcccat gttactagct ttatcgctga tccatcgact
    15961 atcgccgcca ccaacagtga tttaagtacc ttaaaggcaa cggttgagga tggcagtggt
    16021 aacctgatcg aaggtctcac tgtgtacttt gccttaaaaa gcggctctgc cacattaacg
    16081 tcattaacag cggtgaccga tcaaaacgga atcgcgacaa caagcgtgaa aggagcgatg
    16141 acaggtagcg tcacggtaag cgcagtcacg accgctggtg gaatgcaaac agtagatata
    16201 acgctggtgg caggcccagc agacgcctca aagtcagtcc ttaagaacaa tcggtcatca
    16261 ttgaaaggag actttaccga tagtgctgag ctacatcttg ttctgcacga tatatcaggc
    16321 aatccgatca aagtttctga agggctggaa tttgtacagt caggtaccaa cgtgccctat
    16381 gtgcaagtta gtgcaattga ctacagtaaa aatttctcag gcgagtacaa agccactgtt
    16441 acaggaggcg gagagggtat cacaacgctg atccctgtat tgaatggtgt tcatcaagct
    16501 ggtctgagta ccacaataca attcactcgc gcagaagaca aaataatgag cggtacagta
    16561 tcagtcaatg gtactgacct accgacaact acattccctt cgcaggggtt caccggggcg
    16621 tattatcagt tgaataatga caactttgcc ccaggaaaaa cggcggctga ttatgagttt
    16681 tcaagctctg cctcctgggt cgatgttgat gctaccggta aagtgacatt taaaaatgtc
    16741 ggcagcaatt cggaaaggat tacggcgaca ccaaaatcag gaggccctag ctatgtatac
    16801 gaaatccgtg tgaagagttg gtgggtgaac gccggcgagg ctttcatgat atacagcctt
    16861 gctgaaaatt tttgcagcag caatggctac acgctcccca gagcaaacta tttaaaccac
    16921 agtagttccc gaggcatcgg gtcactgtac agtgaatggg gagatatggg gcattacacg
    16981 actgaagctg gttttcaatc aaatatgtat tggtcatcta gtcccgcaaa ctcaagcgaa
    17041 caatacgtag tttccctggc aacaggtgat caaagcgtat ttgaaaagct tgggtttgct
    17101 tatgcgacat gttataaaaa cctctgattt tccgttataa tttcttaaag agtgctccat
    17161 tcggagctct cttttttaca ttccctctat agagcttgcc cttctctcac agaaaatgaa
    17221 taaataaaaa tgcggcaccg ccagaattgc gttcgatgtg actatgatcg tatccggtga
    17281 gtactgcttt gattcaaagg ctggttttct gtgatgattg accagcctca gagtattgat
    17341 gacaccaccg ttcataacag atcggcatta ctatgcataa gtactgtctc atattctcgc
    17401 caaaacactt tttaatgcct ttgcgaattc atccagactt atattgtcaa ttttttgccc
    17461 aacgacgact gactccgaga catcggccat tgcgggccat cgcgttttgt aatcagcata
    17521 aattgcaaca acaggggtat tataggcact tgccatatgt aatattgagg tgtcaggagt
    17581 tattgcgata taagcatctt ttatgattgc tgctgaacgt ttaatcgagg gtggtaagga
    17641 taaacggtag acattattat aacaatccac taatgccctg gctttatctt cacctttggg
    17701 tccatgaacg atcactattg gtatatctgt ttcactttga atttttgcta ttagattttc
    17761 tgcaatcgat aatgaaaatg tacgttcctg cgagctacct tctaaattga acgcaatgta
    17821 agatcctaac gatcgcattt cctcgcgcac ctcatcgagt acatcctcac ttagtggcaa
    17881 ttcatatatt ggcctgactg ctggaaaacc cgcctcacgc atcataaatg cccatgtcat
    17941 aggtacggga gcccggagtt tctgatccat acgggacgcg ttcttgcaca aaggggagta
    18001 gcaattcatg gttataccaa caacctgaaa attcgttttt gctttcaact gactgataaa
    18061 taacatcgtt ttcaggttct ttttacgcat cccctcaata caaagatccg gcgtaccgta
    18121 ttgctgtgtt atgttctttg ctaaatcttt tatttctttt aatgttgcgt gatcctgcat
    18181 agtcattgtg actaatgtta atttagtctg ttcaagttta agcgcattaa agacttctaa
    18241 attaattgtc gacgttacaa ttaaaagatg cttaatttta tgcaattcaa gcgcccgaat
    18301 aacaggaaag atggccatag catcgccaat ctgatcggga atatggatga caacaaagtc
    18361 tgttttttca atattgaaat tataagcttt ataatcgtag taactaaatg caatacgtct
    18421 caacaatgat gctaaaaaca tacctaacct cgcctcccta ctggttataa tgcaatgcag
    18481 tctatcagac tcatcagggt gccattttgt gcatatgcgg acttttatgt ttcatatctc
    18541 taacctgtgg gtcctctgct taatccttaa acaacaccag caactcctgc gctttcatct
    18601 tccatcgaat ttttcatgtt gccgctaatc agccataaaa tcatttgcag atgcgctctg
    18661 tcgaggtagt ctcataaggt tcgtttatag atcgacggca atgtgagtta ccttttccat
    18721 actaattata aaaagacagt acaaacagga tcattatgga ctccacgctc atctccactc
    18781 gtcccgatga agggacgctt tcgttaagtc gcgcccgacg agctgcgtta ggcagcttcg
    18841 ctggtgccgt cgtcgactgg tatgattttt tactctatgg catcaccgcc gcactggtgt
    18901 ttaatcgcga gtttttcccg caagtaagcc cggcgatggg aacgctcgcc gcatttgcta
    18961 cctttggcgt cggattcctt ttccgtccgc tcggcggtgt cattttcggt cactttggcg
    19021 accgactggg acgtaagcgc atgttaatgc tgaccgtctg gatgatgggc atcgcgacag
    19081 ccttgattgg tattcttcct tcattctcga ccattgggtg gtgggcacct attttgctgg
    19141 tgacactgcg tgtcattcag ggatttgccg tcggcggcga atggggaggc gcggcgttgc
    19201 tttccgttga aagtgcaccg aaaaataaaa aagcctttta cagtagcggt gtacaagttg
    19261 gctacggtgt aggtttactg ctttcaaccg gactggtttc attgatcagt atgatgacga
    19321 ctgacgaaca gtttttaagc tggggctggc gcattccttt cctgtttagc atcgtactgg
    19381 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
    19441 aacattatca agctgccgcg aaaaaacgca tcccggttat cgaagcgctg ttacgacatc
    19501 ccggtgcttt cctgaagatt attgcgctac gactgtgcga attgctgacg atgtacatcg
    19561 ttactgcctt tgcacttaat tattcaaccc agaatatggg gctaccgcgc gaacttttcc
    19621 ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt tttgcctggc
    19681 ttgccgatcg ttttggtcgc cgtagggttt atatcacagg tgcgttaatc ggaacgttga
    19741 gcgcatttcc tttctttatg gc
//
LOCUS       DAQSWQ010000076        19739 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.076, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000076 DAQSWQ010000000
VERSION     DAQSWQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.076"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="R8G18_002850"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="R8G18_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HEE9796492.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="R8G18_002851"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="R8G18_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HEE9796493.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="R8G18_002852"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="R8G18_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HEE9796494.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="R8G18_002853"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="R8G18_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HEE9796495.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="R8G18_002854"
     CDS             complement(2270..2761)
                     /locus_tag="R8G18_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEE9796496.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="R8G18_002855"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="R8G18_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HEE9796497.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="R8G18_002856"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="R8G18_002856"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HEE9796498.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="R8G18_002857"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="R8G18_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HEE9796499.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="R8G18_002858"
     tRNA            5405..5492
                     /locus_tag="R8G18_002858"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="R8G18_002859"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="R8G18_002859"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="R8G18_002860"
     CDS             complement(5764..6450)
                     /locus_tag="R8G18_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HEE9796500.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="R8G18_002861"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="R8G18_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HEE9796501.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="R8G18_002862"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="R8G18_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEE9796502.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="R8G18_002863"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="R8G18_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEE9796503.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /locus_tag="R8G18_002864"
     CDS             complement(10902..11732)
                     /locus_tag="R8G18_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEE9796504.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12398..13906)
                     /gene="putP"
                     /locus_tag="R8G18_002865"
     CDS             complement(12398..13906)
                     /gene="putP"
                     /locus_tag="R8G18_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEE9796505.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            14328..18290
                     /gene="putA"
                     /locus_tag="R8G18_002866"
     CDS             14328..18290
                     /gene="putA"
                     /locus_tag="R8G18_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HEE9796506.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="R8G18_002867"
     CDS             complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="R8G18_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HEE9796507.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            19256..>19739
                     /locus_tag="R8G18_002868"
     CDS             19256..>19739
                     /locus_tag="R8G18_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEE9796508.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         5010 a         5256 c         4807 g         4666 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgttact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaagggga taagctgaag gtaccgcgca
    12241 gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgact ggccgccccc
    12301 tgcgcgctcc ctgcaccatt caatttatat ggcgaggcac attaagggag cgcaacccgc
    12361 tgctgaagcc aaacaaaacc gccgcaggct aagtccctta gttttcctgc aaccgtgacg
    12421 gcggagccga atgatagtgc gcatcggcct cggcaaagcg tttttgcatc gccgctgacg
    12481 gcgctttacc cagcaaacta aacactacaa tcccgatact gccgaagatg aagcccggaa
    12541 taatttcgta cagtcccagc cagccgaact gcttccagac gataactgtc agcgcaccga
    12601 tgatcatccc cgccagcgca ccgttgcgcg tcatgcgtga ccacatcacc gagaacagca
    12661 ccaccggacc aaacgccgcg ccaaagcctg cccacgcgta gctcactaag cccagcacgc
    12721 ggttttccgg gtttgccgcc agcgcaatcg ccaccagcgc caccaccagc accatcacac
    12781 gtcctaccca caccagctct ttctggctgg cctgtttacg cagaaacgct ttgtataaat
    12841 cttcggtaat cgcactggag cacaccagca gctggcaact taaggttgac attaccgccg
    12901 ccagaatcgc cgacagcaga atcccggcaa tccacgggtt aaacagaatt tgcgccagtt
    12961 cgataaacac gcgttcagcg ttctggttta ccgcacctgc taccgccgga tgctcgttaa
    13021 agtaagcgat accaaagaag ccaacagcca ccgcccctgc caggcaaagg atcatccagg
    13081 tcatactgat acgacgcgca tggacaatgc tgtggtgaga atctgccgcc ataaaacgcg
    13141 ccaggatgtg cggctgcccg aagtaaccca gcccccaacc catcaatgag ataatggcaa
    13201 caaagttcag ccctttgagc atatccacgt tttcgatgct cttttgtttg atcacttcca
    13261 gcgagtcacc aaagccaccg acactgataa tgacgataac cggcgtcagg atcagggcaa
    13321 aaatcatcag gctggcctgt acagtgtcag tccagctcac cgcgaggaaa ccgccaataa
    13381 aggtgtaaag gatcgtcgac gcagctcccg ccaacagagc cgtttcgtaa ctcatgccaa
    13441 aggtactttc aaacagacgc gcgcctgcca caatgcccga agcgcaataa atggtgaaga
    13501 acagcaaaat aaccagcgcg gagataatgc gcaaaatgcg gcttttatct tcaaaacgcc
    13561 cggtgaaata atccggcaat gttaaggcgt tattattgta ttcggtatgc acacgcaacc
    13621 gcccggccac cagcttccag ttgatccacg cgcctaatgt caggccaatg gcgatccagc
    13681 tttcggaaat cccggaaaga aaaaccgcgc caggcaaccc cattaacagc cagccgctca
    13741 tatccgacgc acccgccgat aatgccgtca cgaatggccc aagactacga ccgcccagaa
    13801 tatagtcgtc aaagtttttc gttgatcgcc aggcgataaa cccaatcaat atcatgccaa
    13861 agatatagac acaaaatgtc accaacatcg gtgtgctaat agccatctaa agtctccaaa
    13921 aaattattat cggcaatgtc gaaacttgcc gttatatctg ccaccggaac ggggtaacag
    13981 agtttatgtt ttaccagggc gaccgtatcc tgccggaagc gctggttatt cacaatcgat
    14041 ttaacacacc atttacatta aattttagtg ctcagcgaca ctatttttca tcaggttgca
    14101 ctctctcaca ttttttgcgg ttgcaccttt caaaaaggtt aactgccgca gagaaaaagt
    14161 ctgagttatt ttttaatcac tgtcatatcg atttctttta ttaacatttc attcattttt
    14221 aagcttgcta cgcaagtcac atttaacatg gttgcacaaa gttgcaacat catggatatt
    14281 tcacgataac gttaagttgc acctttcaga acaacaggag taatggcatg ggaaccacca
    14341 ccatgggggt taagctggac gacgcgacgc gtgagcgtat taagtctgcc gcgacacgta
    14401 tcgatcgcac accacactgg ttaattaagc aggcgatttt ttcttatctc gaacaactgg
    14461 aaaacagcga tactctgccg gagctacctg cgctgctttc tggcgcggcc aatgagagcg
    14521 atgaagcacc gactccggca gaggaaccac accagccatt cctcgacttt gccgagcaaa
    14581 tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc ctatcgccgc ccggaaaccg
    14641 aagcggtttc tatgctgctg gaacaagccc gcctgccgca gccagttgct gaacaggcgc
    14701 acaaactggc gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc agtggtcgtg
    14761 caggaatggt ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa ggcgtggcgc
    14821 tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa agccacccgc gacgcgttaa
    14881 ttcgcgacaa aatcagcaac ggtaactggc agtcacacat tggtcgtagc ccgtcactgt
    14941 ttgttaatgc cgctacctgg gggctgctgt ttactggcaa actggtttcc acccataacg
    15001 aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa aagcggtgaa ccgctgatcc
    15061 gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca gttcgtcacg ggtgaaacca
    15121 tcgcggaagc gttagccaat gcccgcaagc tggaagagaa aggtttccgt tactcttacg
    15181 atatgctggg cgaagctgca ctgactgccg cagacgcaca ggcgtatatg gtttcctatc
    15241 agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg tggcatctat gaaggtccgg
    15301 gcatttcaat caaactgtcg gcgctgcatc cgcgctacag ccgcgcccag tatgaccggg
    15361 taatggaaga gctttacccg cgtctgaaat cactcaccct gctggcgcgt cagtatgata
    15421 ttggtattaa catcgatgcc gaagaggccg atcgtctgga gatctccctc gatctgctgg
    15481 aaaatctctg tttcgagccg gaactggcag gctggaacgg catcggtttt gttattcagg
    15541 cttatcaaaa acgctgcccg ttggtgatcg attacctggt tgatctcgcc acccgcagcc
    15601 gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa atcaagcgtg
    15661 cgcagatgga cggccttgaa ggctatccgg tttatacccg caaggtgtat accgacgttt
    15721 cttatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac ccgcagttcg
    15781 cgacgcacaa cgcccatacg ctggcagcga tttatcaact ggcaggtcag aactactatc
    15841 cgggtcagta cgagttccag tgcctgcatg gtatgggcga gccactgtac gagcaggtca
    15901 ccgggaaagt tgccgacggt aaacttaacc gaccgtgtcg tatttatgcc ccggttggca
    15961 cacatgaaac gctgctggcg tatctggtgc gtcgcctgct ggaaaacggt gctaacacct
    16021 cgtttgttaa ccgtattgcc gacacctctt tgccactgga tgaactggtc gccgatccgg
    16081 tcactgctgt agaaaaactg gcgcaacagg aagggcaaac tggattaccg catccgaaaa
    16141 ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg ctggatctcg
    16201 ctaacgaaca ccgtctggcc tcgctctcct ctgccctgct caatagtgca ctgcaaaaat
    16261 ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg cccgttatta
    16321 accctgcgga accgaaagat attgtgggct ttgtgcgtga agccacgcca cgtgaagtag
    16381 aacaggcgct ggaaagtgcg gttaataacg cgccaatctg gtttgccacg cctccggctg
    16441 aacgcgcggc gattttgcat cgcgctgccg tgctaatgga aagccagatg cagcaactga
    16501 ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc gaagtgcgcg
    16561 aagcggtcga ttttctccac tactacgccg gacaggtgcg ggatgatttc gctaacgaaa
    16621 cccaccgtcc attagggcct gtggtgtgta tcagtccgtg gaacttcccg ctggctattt
    16681 tcaccgggca gatcgctgcc gcactggcgg caggtaacag cgtgctggca aaaccggcag
    16741 aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt gctggaagcg ggtgtacccc
    16801 ctggcgtggt gcaattgctg ccaggtcagg gggaaaccgt gggcgcgcaa ctgacgggtg
    16861 atgatcgcgt gcgcggggtg atgtttaccg gttcaaccga agtcgctacg ttactgcagc
    16921 gcaatatcgc cagccgcctg gacgctcagg gtcgccctat tccgctcatc gctgaaaccg
    16981 gcggcatgaa cgcgatgatt gtcgattctt cagcactgac cgaacaagtc gtcgtggatg
    17041 tgctggcctc ggcgttcgac agtgcgggtc agcgttgttc ggcactgcgc gtgctgtgcc
    17101 tgcaagatga gattgccgac cacacgttga aaatgctgcg cggtgcaatg gccgaatgcc
    17161 ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat agcgaagcga
    17221 aagccaatat tgagcgccat attcagacca tgcgtagcaa aggccgtccg gtgttccagg
    17281 cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt gtcgccccga
    17341 cgctgatcga actggatgac tttgccgaat tgcacaaaga ggtctttggt ccggtgctgc
    17401 atgtggtgcg ttacaaccgt aaccagctac cagagctgat cgagcagatt aacgcttccg
    17461 gctatggtct gacgcttggt gtccatacgc gtattgatga aaccatcgcc caggtcactg
    17521 gctcggccca tgttggtaac ctgtatgtta accgtaatat ggtgggcgca gtggttggtg
    17581 tgcagccgtt cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc ggtccgctct
    17641 atctctaccg tctgctggcg aatcgcccgg aaagtgcgct ggcagtgacg ctcgcgcgtc
    17701 aggatgcaga gtatccggtc gatgcacagt tgaaagccgc attgactcag ccgctaaatg
    17761 cactgcggga atgggcagca aatcgtccag aattgcaggc gttatgtacg caatatggcg
    17821 agctggcgca ggcaggaaca caacgattgc tgccggggcc gacgggtgaa cgcaacacct
    17881 ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga tgatgagcag gatgcgctga
    17941 ctcagctcgc cgccgtgctg gcggtgggca gccaggtact gtggccggat gacgcgctgc
    18001 atcgtcagct agtaaaggca ttgccatcgg cagtcagcga acgtattcaa ctggcgaaag
    18061 cggaaaatat aaccgctcaa ccctttgatg cggtgatctt ccacggtgat tcggatcagc
    18121 ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc aattgtttcg gtgcagggtt
    18181 ttgcccgtgg cgaaagcaat atccttctgg aacggctgta tatcgaacgt tcgctgagtg
    18241 tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa acaggtagcc
    18301 ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc aataataatc
    18361 cgctgcacgt tttcaaccgt ttgattgaaa aatatctcat cgcgcaacgt cgcgcctgtc
    18421 accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca aatcatgaaa
    18481 atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc ggcaatcagc
    18541 gcagatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat taacagcggc
    18601 gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc gcgggagact
    18661 tccagcttca gacggatgta ctcttttatc gccgccagcg gagcgaaatc ttcacgaaac
    18721 gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat atacagcgcc
    18781 tcttttgacg gaaagtaata cagcagattg gtttttgaaa cacccgccaa ctctgcgatc
    18841 tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc tgcgctaaga
    18901 atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt cactgcgcct
    18961 tgcgtcatgc gctctcccct cttgtagatt ggcaacagga tagcaaaacc cccgcccggc
    19021 ctcgaccttc tgcactctca tcgcgcacag atgcatgttt tatgtgcaac tgttttgacc
    19081 gtttagtcca ctttttatca gatattttag ataacgtaat attaacatat taataacatt
    19141 aactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg aaaaggatat
    19201 gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt tgatgatgaa
    19261 aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc acgcgccgca
    19321 gtacatgccg acctttgaac tgaataaagc cattgtgcag aaagcggagc attaccattt
    19381 cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg agttctggga
    19441 tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct cgcgcattca
    19501 gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc gtatggccgc
    19561 aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg gctggcaaaa
    19621 gcccgtgtat gagcagatgg gtatctggcc tggcgatgac tatttctccc gtcgttacga
    19681 ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga aaagcgatt
//
LOCUS       DAQSWQ010000077        19528 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.077, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000077 DAQSWQ010000000
VERSION     DAQSWQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19528
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.077"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..245
                     /locus_tag="R8G18_002869"
     CDS             <1..245
                     /locus_tag="R8G18_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEE9796509.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="R8G18_002870"
     CDS             complement(296..1063)
                     /locus_tag="R8G18_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEE9796510.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="R8G18_002871"
     CDS             1259..2167
                     /locus_tag="R8G18_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEE9796511.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="R8G18_002872"
     CDS             2164..3426
                     /locus_tag="R8G18_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEE9796512.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="R8G18_002873"
     CDS             3423..4061
                     /locus_tag="R8G18_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEE9796513.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="R8G18_002874"
     CDS             4066..4842
                     /locus_tag="R8G18_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEE9796514.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="R8G18_002875"
     CDS             4931..6295
                     /locus_tag="R8G18_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HEE9796515.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6393..7379)
                     /gene="rpoS"
                     /locus_tag="R8G18_002876"
                     /pseudo
     CDS             complement(6393..7379)
                     /gene="rpoS"
                     /locus_tag="R8G18_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
     gene            complement(7442..8581)
                     /gene="nlpD"
                     /locus_tag="R8G18_002877"
     CDS             complement(7442..8581)
                     /gene="nlpD"
                     /locus_tag="R8G18_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HEE9796516.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8721..9347)
                     /gene="pcm"
                     /locus_tag="R8G18_002878"
     CDS             complement(8721..9347)
                     /gene="pcm"
                     /locus_tag="R8G18_002878"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEE9796517.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9341..10102)
                     /gene="surE"
                     /locus_tag="R8G18_002879"
     CDS             complement(9341..10102)
                     /gene="surE"
                     /locus_tag="R8G18_002879"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEE9796518.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10083..11132)
                     /gene="truD"
                     /locus_tag="R8G18_002880"
     CDS             complement(10083..11132)
                     /gene="truD"
                     /locus_tag="R8G18_002880"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEE9796519.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11129..11608)
                     /gene="ispF"
                     /locus_tag="R8G18_002881"
     CDS             complement(11129..11608)
                     /gene="ispF"
                     /locus_tag="R8G18_002881"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEE9796520.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11608..12318)
                     /gene="ispD"
                     /locus_tag="R8G18_002882"
     CDS             complement(11608..12318)
                     /gene="ispD"
                     /locus_tag="R8G18_002882"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEE9796521.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12337..12648)
                     /gene="ftsB"
                     /locus_tag="R8G18_002883"
     CDS             complement(12337..12648)
                     /gene="ftsB"
                     /locus_tag="R8G18_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEE9796522.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12842..13165)
                     /locus_tag="R8G18_002884"
     CDS             complement(12842..13165)
                     /locus_tag="R8G18_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HEE9796523.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13215..13820)
                     /gene="cysC"
                     /locus_tag="R8G18_002885"
     CDS             complement(13215..13820)
                     /gene="cysC"
                     /locus_tag="R8G18_002885"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEE9796524.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13820..15247)
                     /gene="cysN"
                     /locus_tag="R8G18_002886"
     CDS             complement(13820..15247)
                     /gene="cysN"
                     /locus_tag="R8G18_002886"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEE9796525.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15249..16157)
                     /gene="cysD"
                     /locus_tag="R8G18_002887"
     CDS             complement(15249..16157)
                     /gene="cysD"
                     /locus_tag="R8G18_002887"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEE9796526.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16409..17446
                     /gene="iap"
                     /locus_tag="R8G18_002888"
     CDS             16409..17446
                     /gene="iap"
                     /locus_tag="R8G18_002888"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HEE9796527.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17467..17739
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17528..17554
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaac"
     gene            complement(17836..18129)
                     /gene="cas2"
                     /locus_tag="R8G18_002889"
     CDS             complement(17836..18129)
                     /gene="cas2"
                     /locus_tag="R8G18_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HEE9796528.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18126..19049)
                     /gene="cas1e"
                     /locus_tag="R8G18_002890"
                     /pseudo
     CDS             complement(18126..19049)
                     /gene="cas1e"
                     /locus_tag="R8G18_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(19046..>19528)
                     /gene="cas6e"
                     /locus_tag="R8G18_002891"
     CDS             complement(19046..>19528)
                     /gene="cas6e"
                     /locus_tag="R8G18_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HEE9796529.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4605 a         5267 c         4896 g         4760 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt acgcggaaca gcgcttcgat attcagcccc
     6421 tgcgtttgca ggatttcacg caaacggcgc aggccttcaa cctgaatctg gcgaacacgt
     6481 tcacgggtga ggccaatttc acgacctaca tcttccagtg ttgccgcttc gtaccccagc
     6541 aaaccgaatc gacgtgccag tacttcacgc tgtttggcgt tcagctcgaa cagccatttg
     6601 acgatgctct gcttcatatc gtcatcttgc gtggtatctt ccggaccgtt ctctttttca
     6661 tcggccagga tgtccagcaa cgctttttcg gaatcaccac ccagcggggt gtctaccgag
     6721 gtaatgcgct cgttaagacg aagcatacgg ctgacgtcat caactggctt atccagttgc
     6781 tctgcgatct cttccgcact tggttcatgg tccagcttat gggacaactc acgtgcggtt
     6841 cgcaggtaaa cgttcagctc ctttacgatg tgaatcggca aacgaatagt acgggtttgg
     6901 ttcataatcg cccgttcaat cgtctggcga atccaccagg ttgcgtatgt tgagaagcgg
     6961 aaaccacgtt ccgggtcaaa cttctctacc gcgcggatca gccccaggtt gccctcttcg
     7021 ataaggtcca gcaacgccag accacgattg ccataacggc gggcaatttt taccaccaga
     7081 cgcaagttac tctcgatcat ccggcggcga gaggcgacat ctccacgcag tgcgcgacgc
     7141 gcaaaataaa cttcttcttc ggccgttaac agtggtgaat aaccaatctc accaaggtaa
     7201 agctgagtcg cgtccaacac acgctgtgtg gctccctgcg ataacagttc ctcttcggcc
     7261 aaatcgttat cactgggttc ctcttctact aaggcctttt cgtcaaaaac ctcaactccg
     7321 ttctcatcaa attccgcatc ttcatttaaa tcatgaactt tcagcgtatt ctgactcata
     7381 aggtggctcc tacccgtgat cccttgacgg aacattcaag caaaagcctg gttccgccga
     7441 tttatcgctg cggcaaataa cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa
     7501 aatgcaagcg tgttgaactg gttccggtgc tacccatggt tgctattttt tgccccgcct
     7561 tcacttcttg ttgttcccgg accagcattg tgtcgttatg ggcgtaggca ctcaggtaat
     7621 catcattatg tttgatgata atcagattac cgtagccgcg cagcgcgtta ccggcataaa
     7681 caacgcggcc atctgcggtc gcgataattg cctgtccttt gctgcctgcg atatcaatcc
     7741 ccttgttgcc cccctcagaa gcgccaaagg tttcgatcac tttgccctca gtcggccagc
     7801 gccaggtgga gataggcgta ctggttgatg tactgctgac agtcggctcg gttgtgcttg
     7861 ctgttggtac cgttacaggc gctgtgactg tggtcgcagt tggcttgttg ttcggcaaca
     7921 ttttgttagc actctgttca cccgaagact cagaatacgt aattgtcggt tgcgacgcaa
     7981 cagcaacggt ggaattttgt gcaggcttga tcacaactcc ttgctctgct gcgtcggcct
     8041 gggtaatggc atttccgcca gtgattggcg taccggaagc attacccacc tgcaaggtct
     8101 gaccaacgtt cagtgcgtat ggtgcctgaa tattgttgcg ctgagcaagg tcacggaaat
     8161 cgttgccagt aatccaggcg atatagaaaa gtgtgtcgcc ttttttcacg gtataggtac
     8221 tgccgctata actgcctttc ggaatgttcc catactgacg gttatagacg atgcgtccgt
     8281 tttccatctg taccggctgc tgagctactg gctgcactgg ctggatttgc ggttgttgag
     8341 tagcctgaat ttgtggctgc tgtaccggct gaatttgcgg ttgctgggct gtagacgtcg
     8401 tccccatttt cggcggcggc gtaatcaaca taccagaatt agtatttgca ggcgcattgc
     8461 cattaacgga gctgaccggt gccggtggat ttgaagtgtc agaacagcct gccagccata
     8521 gcgaaaccag tgacaaagca gcaatgcggc gaacggtgaa ttttgggctt cccgcgctca
     8581 tttatccccc aggaaaaatt ggttaataac cagtgacata attaccgtgc aaggcaccct
     8641 actgaacact ggaaaagatg ttcacgatac gctgacctgc ggcaaaataa ccaggaaaaa
     8701 tccaggtatt tcctcacgtt ttaagccagc tcacccttca ctaaagggac aaagcgcacg
     8761 gcctccacgg tatcgataat aaattcgcct ccccgacgac gcacccgttt caaatactgg
     8821 tgctcctccc ctacgggtaa gacgagaatc ccgccttcgt ccagctgcgt cattagcgca
     8881 gttggaattt ccggcggtgc cgccgtaaca atgatagcgt caaacggcgc acgtgcctgc
     8941 caaccttgcc atccatcgcc atgacgggtt gaaacattat gtaaatcaag atttttcagg
     9001 cggcgacgtg cctgccactg caagccttta atccgttcaa ccgagcaaac atgctggaca
     9061 agatgcgcta ggattgccgt ttgatatccc gaaccagtgc caatttccag cacccgcgac
     9121 tgcggcgtca gctcgagtaa ttcggtcatt cgcgccacca tatacggctg cgaaattgtc
     9181 tgcccctgac ctatcggcaa ggcgatattg tcccaggctt tttgttcaaa cgcttcatca
     9241 acgaattttt cacgcggcac ggcggcaagt gcattcagca cctgctcatc ctgaatacct
     9301 tgcgcacgta attgatccag aagtgcttgt acgcgtctgc ttaccattgc gtgccaactc
     9361 ccacgctgtt taaccagtct gaaaccacat cttgcgcgct atgcgcagtt aaatccacat
     9421 gcagcggcgt gatggagaca tagccctcat ctaccgcagc aaaatcggtc cccggaccgg
     9481 catcacattt accgcccggc gggccaatcc agtacagcgt attgccgcgc ggatcttgct
     9541 gcgggatcac ctgatctgcc ggatgtcgtg taccgcagcg cgtcacgcga atacctttga
     9601 tttgatccaa gggtaaatcc ggaacgttaa tattaagaat acgtccggtg cgcagcggct
     9661 ctttacacag tgcgcgcaaa attgaacagg ttaccgccgc ggcagtgtcg taatgtttat
     9721 gcccgtcaag cgagacggcc agcgccggaa aacctaaatg acggccttcc atcgcggcgg
     9781 ctaccgtacc ggaataaata acgtcatccc ccagattcgg cccggcgtta atgccggaca
     9841 caacaatgtc cgggcgcgga cgcatcagag cattcacgcc aagatagacg caatcggtcg
     9901 gggttcccat ttgcacagca atatcaccat tttcaaaggt aaacgtgcgc agggaggatt
     9961 ccagtgtcag agaatttgaa gcgccgctgc ggttacgatc gggggcgacc acctgaacgt
    10021 cagcaaactc acgcaaggct ttcgccagcg tttgtatacc gggtgcatgt accccgtcat
    10081 cattactcag caatatgcgc ataatcacct gttgtgttga taagttccct gacaacgctg
    10141 gttgcaaaac tacccgccgg aagccagaaa cggatctcta cggtgacgtc atcccaccaa
    10201 ttccagctta attgttgcgg atacagcaac atcgctctgc gcgcggcttc aactttttcg
    10261 cgcaccagta aagcttgtaa ttcagtttct gcggcgacag ctgccttttc gaatgccagc
    10321 gcttcacgct gagttcccca ttcgccacta cccggcaatg cggcggttat catcagctct
    10381 ttatcgttga cgcgacgctg taattccgcc agttcttcgg tggttgcgac aaaccagcta
    10441 ccacgtccgg ctaattgtag cgcatcgccg tcaacaactt gattaacgtc tgcttttttg
    10501 aggcgctcag caacaatctg attaaacaac gcactgcggg ctgccgacaa ccaaaaactc
    10561 cgtttattgc gatcgcgcac cggagtattg gtttgcgccc agcgctgcgc cccctgcaag
    10621 ttgctaccgc caatcccaaa acgttgggca ccgaagtagt tcggtacacc ttttacgcaa
    10681 atatcgatca gacgttgttc aacgtcatcg cgattgctca cttcgcgcag aaccagggta
    10741 aaggcgttac ctttcagcgc gcctaaacgc agcttgcgct tgtgccgcgc atactccagc
    10801 acctggcagc cttccagttg aaaggcgctc agatcgggca tttccttgcc tggcacgcga
    10861 gcgcataacc actgttccgt aacagcatgt ttgtcttttt gcccagcgaa gctgacttca
    10921 cgggcatgaa ttttcaggaa tttcgccagt gcatccgcca caaaacgggt attgcagccg
    10981 tttttgagga ttctaaccag aatatgctca ccttcaccat caggctcaaa gcccaaatct
    11041 tccaccacca caaagtcttc cggattggct ttcagcagcc cggtgccttg cggtttaccg
    11101 tggaggtaag tgagattatc aaactcaatc attttgttgc cttaatgagt agcgccaccg
    11161 cttcacaggc aatcccttcc ccacgtccgg taaatccaag tttttccgta gtagtggctt
    11221 tcacgttaac atcatccata tggcagccga gatcttcggc aataaacacg cgcatttgtg
    11281 gaatgtgcgg caacatcttc ggtgcctgag cgataatagt gacatcgacg ttgcccaggg
    11341 tataaccctt cgcctgaata cgacgccagg cttcgcgtag cagctcgcgg ctgtcggcac
    11401 ctttaaatgc cggatcggta tccgggaaca gcttgccgat atcccccagc gccgccgcgc
    11461 caagcaatgc atcggtcaac gcatggagcg ccacgtcgcc atcagaatgc gccagcaatc
    11521 ctttttcgta aggaatgcgt acgccaccaa tgataattgg gccttcaccg ccaaaggcat
    11581 gtacgtcaaa accgtgtcca attcgcatta tgtattctcc tgatggatgg ttcgggtgag
    11641 gtaaaactcg gccagtgcca aatcttccgg gcgcgtgact ttaatgttat ccgcacggcc
    11701 ttcgaccaac tgaggatgga atccgcaata ttccagcgcc gaggcttcgt cggtaatagt
    11761 cgcgccttca tttagagcgc gcgtcagaca gtcatgtaac agctcacgag ggaaaaattg
    11821 cggcgtcagc gcgtgccata agccgttgcg atcaacggta tgagcaatgg catttttgcc
    11881 cggttcggca cgtttcatcg tatcgcgcac tggtgcggct aggatccctc ccgtgcggct
    11941 ggtttcgctc aacgccaaca atcgcgcgag gtcatcctga tgcagacaag gacgagcggc
    12001 gtcatgcacc aatacccact gcgcgtcgcc agcggctttc aaacctgcca gcacggaatc
    12061 ggcacgctca tcaccgccat ctacaacggt gatttgcgga tgattcgcca gaggaagttg
    12121 tgcaaaacgg ctatcgccag gacttatggc aatgacgaca cgtttcaccc ggggatgcgc
    12181 cagcagcgca tgcaccgagt gttcaagaat ggtttgatta ccgattgaga gatattgctt
    12241 aggacattcc gtttgcattc gacggccaaa tccggccgcc ggaaccacgg cgcaaacatc
    12301 caaatgagtg gttgccatgt taattcccgg gctgatttat cgattgtttt gccccgcaga
    12361 ctgtgcgcgc ttcgacgcgt caggcaccag acgataaaaa gtttcgcccg gcctggtcat
    12421 gctgagttca ttacgcgcac gctcttcgag cgcctcctgg ccgccattga gatcgtcaat
    12481 ttcggcaaaa agttgatcgt ttcgcgcttt aagtttcgcg tttgtagctt gctgtgccgc
    12541 cacatcatca ttgacgcggg tatagtcatg tataccgttc ttaccgaacc acagcgaata
    12601 ctgtagccag accagaatag ccagcaacag cagcgttagt ttacccatcc tgccccctga
    12661 aaaacggcat catcatccca tgcatccgaa gacgactcta catcctctgt tggggatacc
    12721 gcgacaacgc gggcaaatgt accacatttg tccattgtta cgtataccca gggcgtgcag
    12781 aacataatct cattattagt tacggtttga attatgaaca gaggagacaa gaaagtacaa
    12841 attagcccag tagccacata aacagtgcgc caaacataat gcctactgtc accagggtga
    12901 aaacaatact gtagcgtagc tttccgtcca tcaatgaatg cagcgcaatc cccaccacta
    12961 ccgcgacggg catcagcgcc agaaagaaag gccaggtgta gataaagaag aacagcgtgt
    13021 tagagccata aatcaacatc ggcatcgcca gcgcaaataa ccaggagata aaaccgacca
    13081 cggcaccagg cagtgaccat gtggtttctt catcctcagt aaggctgtcg ttatttgtta
    13141 gtgtaatgtt atggctatta cgcatatttg atcctgttac tttgacgaac cgggcatgga
    13201 aacccggtgg tgtctcagga tctgataata tcgttctgtc tcaacagatc taataattgc
    13261 tgtaccaaat ttgttactaa ttgttcacca ttgagatgaa tttctgccga ttcaggcgct
    13321 tcgtaaacgg aatctattcc cgtaaagttg cgcagttcac cggcacgcgc tttcttatat
    13381 aagccttttg gatcgcgggc ttcgcaaatc gccagcggcg tatcgacaaa cacttcgata
    13441 aagcgccctt ctcctacgcg ttcacgaacc atctggcgtt cggcgcggtg tggcgagata
    13501 aatgcggtca gcaccaccag tccggcttca accatcaaat tcgccacttc accgacgcga
    13561 cggatattct ctttacgatc ggcatcgcta aaaccgagat cgctgcataa tccgtggcga
    13621 acattatcgc catccagcag atacgtactg acgccgagtt tatgtaacgc ctcctccagc
    13681 gccccggcga ccgttgattt accggacccg gagaggccgg taaaccacag cactacacca
    13741 cgatgaccgt ggtgtagctc gcgttgttgc acagtgaccg gatggctatg ccagacgacg
    13801 ttttcgtcat gcagcgccat tatttatccc ccagcaaatc gcgcgcgccc cagtgcggaa
    13861 agtggcgacg aaccagagca ttcaattcca gttcgaatgc actgaattca gatggcgcag
    13921 cagttgcctg gctaactggc tcgtgcacca taccggcacc cacggtcaca ttgctcaggc
    13981 gatcgataaa aatcagccca cccgtcaccg gattttgttg ataacgatct aacaccagcg
    14041 gctcgtcaaa agtgagatcc acgaggccga tcccattcag tggcaggttt tcaacttcac
    14101 gctgggtaag gttattaata tcaacctgat agcgaatgcc atcaacacgc gcgcgcgtct
    14161 tcttaccggc aattttgatg tcgtaactct gccctggaga aagcggctgt tccgccatcc
    14221 ataccacatc caccgacgcg ctctgcaccg ccggtaacgc ttcgtctgcc gccagcagca
    14281 gatcgccacg gctgatgtcg atctcatccg tcagcaccag ggtgatcgct tctccggcaa
    14341 aggcttcttc gcgatcacca tcaaaagtca cgatccgcgc gacgtttgat tccacaccag
    14401 agggcagcac ttttacacgt tgcccgactt ccacgcgacc ggatgccagc gttccggcgt
    14461 agccacgaaa atcgagattc gggcgattaa cgtactgcac cgggaagcgc attggctgag
    14521 catccaccac tcgctggatc tccacggttt ccagcacttc gagcagtgtc ggaccgctgt
    14581 accacggcat actttcactt tgcgatgcca cgttgtcgcc ttccagtgca gagagcggca
    14641 caaagcggat atccagatta cccggcagct gcccggcaaa ggtcagataa tcttcacgaa
    14701 tacgggtgaa cgtctcttca ctgtaatcca ccagatccat tttgttgatc gccacgacca
    14761 gatgtttgat ccccaacagt gtggagataa aactgtgacg acgggtttga tcgagcacgc
    14821 ctttacgggc atcgatcagt aagatcgcca gttcacatgt cgatgcgcca gtcgccatat
    14881 tgcgagtgta ctgctcgtgc cctggggtgt cggcgataat aaatttacgc ttctcggtag
    14941 agaaatagcg gtaggccaca tcaatagtga tgccctgttc gcgctcagct tgcaggccgt
    15001 ccaccagcag agccagatcc agcttttcgc cctgggtacc gtgacgctta ctgtcgttat
    15061 gcagcgatga gagctgatct tcatagattt ggcgagtatc gtgcagcagg cgaccaatca
    15121 gagtgctttt gccgtcatcg acgctgccac aggtcagaaa acgcagcagg cttttatgtt
    15181 gttgcgcaat catccaggct tcgacgccgc cttcattggc gatttgttgt gcaagtgcgg
    15241 tgttcatctt aaaaataccc ctgacgtttt ttcagctcca tagatcccgc ctggtcgcgg
    15301 tcaatcacgc ggccctgacg ttcactggtg gtggaaacca gcatctcttc gatgatttcc
    15361 ggcagtgttt gtgcatttga ctccaccgca ccggtcagcg gccagcagcc cagcgtacgg
    15421 aaacgcacca tccgtttttt aatcacttcg cccggttgca ggtcgatacg gttgtcatca
    15481 atcatcatca acataccgtc gcgttccaga accggacgtt ccgcagcgag atacagcggc
    15541 acaatgtcga tattttccag ccagatgtat tgccagatat cctgctcggt ccagttagag
    15601 agtgggaaga cgcggatgct ttcgcctttg ttaatttgcc cgttgtagtt gtgccacagc
    15661 tccgggcgct gatttttcgg atcccagcga tggaagcggt cacggaaaga gtagattcgc
    15721 tctttagcgc gggatttctc ttcgtcacgg cgcgcaccac cgaaggcggc atcaaaaccg
    15781 tatttgttca gcgcctgttt caggccttca gttttcataa tatcggtatg tttcgcgctg
    15841 ccgtgcacga atggattaat ccccatcgcc acgccttccg ggtttttatg caccagcagc
    15901 tcgcagccgt aggctttcgc cgtacgatcg cggaactcat acatctcgcg gaatttccag
    15961 ccggtatcga catgcagcaa cgggaaaggc agcgtacctg gataaaacgc cttgcgcgcc
    16021 aaatgcagca tgacgctgga atctttaccg atagagtaga gcatcaccgg atttgagaat
    16081 tctgccgcca cctcgcgaat aatgtggatg ctttccgcct ccagttgccg caggtgagta
    16141 agtcgtattt gatccataac cgttcctttg caataccgct attttcttgc catcagatgt
    16201 ttcgactata gggagcgtaa gagaacgaat gaaattacca attagaatga gtagttcctt
    16261 aacggaataa cgatttggca aagctaatat caaaaagtgc ttaaggcacc ggatttctgg
    16321 cgtttaggaa gatttgaaat tgttttagcg cagcggcagt ttcatactat ggcggtaaaa
    16381 aaatttgcat ggtatttaag gactcactat gttttccgca ttgcgccacc gtaccgctgc
    16441 cctggcgctc ggcgtatgct ttattctccc cgtacacgcc tcgtcaccta aacctggcga
    16501 ttttgctaat actcaggcac gacatattgc tactttcttt ccgggacgca tgaccggaac
    16561 tcctgcagaa atgttatctg ccgattatat tcgccaacag tttcagcaaa tgggttatcg
    16621 cagtgatatt cggacattta atagtcggta tatttatact gcccgcgata atcgtaagag
    16681 ctggcataac gtgacgggaa gtacggtgat tgccgctcat gaaggcaaag cgccgcagca
    16741 gatcatcatt atggcgcatc tggatactta cgccccgctg agcgatgctg acgccgatgc
    16801 caatctcggc gggctgacgt tacaaggaat ggatgataac gccgcaggtt taggtgtcat
    16861 gctggaactg gcagaacgcc tgaaaaatac gcctaccgag tatggtattc gatttgtggc
    16921 gaccagcggc gaagaggaag gaaaattagg cgctgagaat ttactcaagc ggatgagtga
    16981 caccgaaaag aaaaatacgc tgctggtgat taatctcgat aacttaattg ttggcgataa
    17041 attgtatttc aacagcggtg taaaaacccc tgaagcagta aggaaattaa cgcgcgacag
    17101 ggcgctggca attgcgcgca gtcacggaat agccgcaacg accaatccgg gtttgaataa
    17161 aaattatccg aaaggcactg ggtgttgtaa tgacgcagaa atattcgaca aagcgggcat
    17221 tgctgtactt tcggtggaag cgactaactg gaatcttggg aataaggatg gttatcagca
    17281 acgcgcaaaa acagccgcat tccctgcggg aaatagctgg catgacgtaa gactggataa
    17341 tcaacagcat attgataagg cacttcctgg aagaatagaa cgtcgctgcc gtgacgttat
    17401 gcggataatg ctacctctgg tgaaggagtt ggcgaaggcg tcttgatggg tttgaaaatg
    17461 ggagctgggt gttctaccgc aggggcgggg aattctaagt gatatccatc atcgcatcca
    17521 gtgcgcccgg tttatccccg ctggcgcggg gaactcttac tgcttggtat gcggaatcac
    17581 accctgaacg gtttatcccc gctggcgcgg ggaacaccct gacgcgccgc agtatttatc
    17641 tgctctggcc ggtttatccc cgctggcgcg gggaacacgg gtgcgtgtgg ctgccagtgc
    17701 cggagaacgg cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17761 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17821 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17881 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17941 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    18001 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    18061 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18121 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18181 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18241 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18301 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18361 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18421 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18481 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18541 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18601 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18661 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18721 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18781 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18841 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18901 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18961 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    19021 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    19081 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19141 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19201 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19261 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19321 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19381 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19441 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19501 ggcgttcctg cgacaggaca aaaaagcg
//
LOCUS       DAQSWQ010000078        19376 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.078, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000078 DAQSWQ010000000
VERSION     DAQSWQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.078"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            376..825
                     /gene="alaE"
                     /locus_tag="R8G18_002892"
     CDS             376..825
                     /gene="alaE"
                     /locus_tag="R8G18_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HEE9796530.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(862..1206)
                     /locus_tag="R8G18_002893"
     CDS             complement(862..1206)
                     /locus_tag="R8G18_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HEE9796531.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1358..1687
                     /locus_tag="R8G18_002894"
     CDS             1358..1687
                     /locus_tag="R8G18_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEE9796532.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1935..2180
                     /gene="nrdH"
                     /locus_tag="R8G18_002895"
     CDS             1935..2180
                     /gene="nrdH"
                     /locus_tag="R8G18_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEE9796533.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2177..2587
                     /gene="nrdI"
                     /locus_tag="R8G18_002896"
                     /pseudo
     CDS             2177..2587
                     /gene="nrdI"
                     /locus_tag="R8G18_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2560..4704
                     /gene="nrdE"
                     /locus_tag="R8G18_002897"
     CDS             2560..4704
                     /gene="nrdE"
                     /locus_tag="R8G18_002897"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEE9796534.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4714..5673
                     /gene="nrdF"
                     /locus_tag="R8G18_002898"
     CDS             4714..5673
                     /gene="nrdF"
                     /locus_tag="R8G18_002898"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEE9796535.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6029..7231
                     /gene="proV"
                     /locus_tag="R8G18_002899"
     CDS             6029..7231
                     /gene="proV"
                     /locus_tag="R8G18_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HEE9796536.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7224..8288
                     /gene="proW"
                     /locus_tag="R8G18_002900"
     CDS             7224..8288
                     /gene="proW"
                     /locus_tag="R8G18_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HEE9796537.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8346..9338
                     /gene="proX"
                     /locus_tag="R8G18_002901"
     CDS             8346..9338
                     /gene="proX"
                     /locus_tag="R8G18_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HEE9796538.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9629..10813
                     /locus_tag="R8G18_002902"
     CDS             9629..10813
                     /locus_tag="R8G18_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9796539.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10937..11674
                     /gene="ygaZ"
                     /locus_tag="R8G18_002903"
     CDS             10937..11674
                     /gene="ygaZ"
                     /locus_tag="R8G18_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HEE9796540.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11664..11999
                     /gene="ygaH"
                     /locus_tag="R8G18_002904"
     CDS             11664..11999
                     /gene="ygaH"
                     /locus_tag="R8G18_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HEE9796541.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12090..12620
                     /gene="emrR"
                     /locus_tag="R8G18_002905"
     CDS             12090..12620
                     /gene="emrR"
                     /locus_tag="R8G18_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HEE9796542.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12747..13919
                     /gene="emrA"
                     /locus_tag="R8G18_002906"
     CDS             12747..13919
                     /gene="emrA"
                     /locus_tag="R8G18_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HEE9796543.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13936..15474
                     /gene="emrB"
                     /locus_tag="R8G18_002907"
     CDS             13936..15474
                     /gene="emrB"
                     /locus_tag="R8G18_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HEE9796544.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSITLALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15538..16053)
                     /gene="luxS"
                     /locus_tag="R8G18_002908"
     CDS             complement(15538..16053)
                     /gene="luxS"
                     /locus_tag="R8G18_002908"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEE9796545.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16203..17759)
                     /gene="gshA"
                     /locus_tag="R8G18_002909"
     CDS             complement(16203..17759)
                     /gene="gshA"
                     /locus_tag="R8G18_002909"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEE9796546.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17832..18260)
                     /locus_tag="R8G18_002910"
     CDS             complement(17832..18260)
                     /locus_tag="R8G18_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEE9796547.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18257..18823)
                     /gene="yqaB"
                     /locus_tag="R8G18_002911"
     CDS             complement(18257..18823)
                     /gene="yqaB"
                     /locus_tag="R8G18_002911"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HEE9796548.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19104..19180)
                     /locus_tag="R8G18_002912"
     tRNA            complement(19104..19180)
                     /locus_tag="R8G18_002912"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19144..19146),aa:Arg,seq:acg)
BASE COUNT         4616 a         5009 c         4886 g         4865 t
ORIGIN      
        1 actggtgctt tctgctgtta atgtacattt tctggcatca aaaatgctgt aaatgacacg
       61 gtcacgacat acaccgaatt ctctacgctt atagcgttac ctcaccccca aactttcatt
      121 agtgaaatta aaggcaaggt catatctaat tgatttaatt gaaaataaat catatcggca
      181 tgtaaaaatc cacaatgtac aaaaaacgag ccgttacgga atattttatc tacaaaaact
      241 gactaaataa aaatttttca ctaattgaat agtcatagcc agcgatatac gctatgcgaa
      301 aatgcagatg gcaatgagat ccactgcttt catctccatt aacatcccat tacgctttta
      361 ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc gtcatgcagt tgcagatacg
      421 ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta ttgaagtttt cctctccgga
      481 atgagcttcg aacagtcttt ttattccaga ttggtagcga ttccggtgaa catcttaatt
      541 gcatggccat acggtatgta ccgcgatcta tttatgcgcg cggcacgcaa agttagcccg
      601 tcgggctgga taaaaaatct ggctgatatc ctggcttatg tgacgttcca gtcaccggtg
      661 tatgtggcga tcttgttagt ggtgggcgca gactggcatc agattatggc ggcggtcagt
      721 tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg gctacttcct cgattattgc
      781 cgccgactgt ttaaagtcag ccattaccag caggtaaaag cctgactctt cttttcgcga
      841 ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat aaaccattca aataacgttc
      901 aagcgcaaca cgcgaactga agccatgcgg gataccgtaa tgctcgtgtc gctcgcctgc
      961 taaatagagc ggaaattgtt gataatgatc gcaagtttta atatcggaag cgggttcagc
     1021 cagtgtcgaa ctacgttctt ttaatgttgg atgaattacc agtgcggtac gtcccattcg
     1081 cgcttcgcga ttaacataga cataattttc cccacggcgg taaccataag ctttctgggt
     1141 taccacatcg acagtaaaac cgactttttc aagtacgcgc gccacctcgt ctggtcgtaa
     1201 atacatattt gatcctcgtt attatcaacc gcgggcctac cttacctgat tgcgcattca
     1261 caacgctttc agaaaagtcc ataaagccgc gatgaacctc agttaagaaa attatggtct
     1321 acactgaaaa ttacatcgaa ttctaatggg ggatcatatg tttaaccgac cgaaccgcaa
     1381 cgacgttgat gatggcgtgc aggatatcca gaatgatgtc aatcaattag ctgacagcct
     1441 cgaatctgta ttgaagtcct ggggcagcga cgccaaaggg gaagctgaag ccgcacgcag
     1501 taaagctcag gcattgctga aagaaaccag ggcgcgaatg catggtcgta ctcgcgtcca
     1561 gcaagccgca cgcgatgccg ttggctgcgc ggattctttt gttcgtgaaa gaccctggtg
     1621 tagcgtgggt acagcagctg cggtaggtat ttttatcggc gcactgttaa gcatgcgcaa
     1681 atcgtagtgc aaaaatgata ataaatacgc gtctttaacc ccgaagcctg tcttcggggt
     1741 ttctttttgc ctggtgaatc acaaaaatcc ccctcccccg tcacgctcat atctagggta
     1801 atttcgatca ctatttgcta tatattgtgt ggttgaatct tttttcaact acatctagta
     1861 tctctgtatc aacagagaga caacccgacg cgtatcatcg cgccgtatct tcattttaaa
     1921 cggaaatacg aatcatgcgc ataactattt acactcgtaa cgattgcgtt cagtgccacg
     1981 ccaccaaacg ggcgatggaa aaccggggct ttgattttga aatgattaat gtcgatcgcg
     2041 ttcctgaagc agcggaagcg ttgcgtgctc agggctttcg tcagttgccg gtagtgattg
     2101 ctggcgatct tagctggtct ggtttccgtc cggacatgat taaccgtctg catccagcgc
     2161 cacacgcggc cagtgcatga gccagctcgt ctacttctcc agcagctccg aaaacacgca
     2221 gcgttttatc gaacgtttag gtctgcccgc ggtgcgcatc ccgctcaatg agcgggaacg
     2281 gattcaggta gacgagcctt acatcctgat cgtgccctct tacggcggcg gcggtacggc
     2341 tggcgcggtg ccacgatagg taattcgctt tttaaacgac gagcacaacc gggcgttgct
     2401 tcgcggcgtt attgcttctg gtaatcgcaa ctttggtgag gcgtatggcc gcgccggaga
     2461 tgtgattgcc cggaaatgcg gcgtgccgtg gctgtaccgt tttgaactca tgggtacgca
     2521 aagcgatatc gaaaacgttc gtaaaggagt aaccgaattt tggcaacgac aaccgcagaa
     2581 tgcctgacgc aggaaacgat ggattaccac gcgctgaatg cgatgcttaa cctctacgat
     2641 agcgcaggtc gcattcagtt cgataaagac cgccaggccg ttgacgcctt tatggcgacg
     2701 catgtgcgtc cgaacagtgt gaccttcagt agccagcagc agcgcctgaa ctggctggtc
     2761 aacgaaggtt actatgatga aagcgttctt aatcgctact ctcgcgactt tgtcattacg
     2821 ctgtttgccc acgcacacac cagcggtttt cgtttccaga cattcctcgg ggcatggaag
     2881 ttttacacca gctatacgtt gaagacattc gacggtaaac gttatctgga agattttgcc
     2941 gatcgagtca cgatggtggc gctgacgctg gcacaaggcg atgcgacgct ggcgttgcaa
     3001 ctgaccgatg aaatgctgtc aggacgcttt cagccagcca cgccgacatt cctcaactgc
     3061 ggtaagcagc agcgcggcga actggtttcc tgttttctgc tgcgtattga agacaatatg
     3121 gagtcgattg gccgggcggt aaattccgca ctgcaactgt cgaaacgcgg cggcggcgtg
     3181 gcatttttgc tgtcgaatct gcgagaagcg ggcgcgccaa ttaaacgtat tgaaaatcaa
     3241 tcttctggcg taattccggt gatgaaaatg ctggaagacg cattttccta tgccaaccaa
     3301 ctcggcgcgc gtcagggggc aggtgcagtc tatttacatg ctcatcatcc cgatattctg
     3361 cgttttctcg atacaaaacg ggaaaatgcc gacgaaaaaa tccgcattaa aacactgtcg
     3421 ctgggcgtgg tgatcccgga tatcactttc catctggcaa aagagaatgc gcagatggcg
     3481 ctgttttcgc cctatgacgt tgagcgagtt tatggcaagc cgtttgccga tatcgccatc
     3541 agcgaacact atgacgaact ggttgccgat gaacgcatcc gcaaaaaata cctcaacgcc
     3601 cgtgatttct tccagcggct ggcagaaatc cagtttgagt ccggctatcc ctacatcatg
     3661 tatgaagaca cggtaaaccg tgctaaccct gtcgccgggc gcataaatat gagtaatctc
     3721 tgctcagaga ttttgcaggt caactgtgcc tcagagtatg acgagaatct cgactatgcc
     3781 cgcacaggcc atgatatctc ctgcaattta ggttcgttga atattgcgca caccatggat
     3841 tcccccgatt ttgcccgcac ggtagagact gccgtgcgcg gtttaacggc ggtgtcagat
     3901 atgagtcata tccgcagcgt gccgtccatc gaagccggaa atgccgcctc acacgccatc
     3961 ggactggggc agatgaattt acacggctat ctggcgcgag aaggcatcgc ttatggttcg
     4021 ccggaagcac tggatttcac caatctctat ttctatgcca tcacctggca cgcactgcgt
     4081 acctcgatgt tgctggcgcg cgaacgcggt gaaaccttcg ccgggttcaa acagtcacgt
     4141 tatgccagtg gtgaatattt tagccaatat ctgcaaggta actggcagcc gaaaacggcg
     4201 aaagttggcg aactgtttgc ccgcagcggt attacgttac ctacccgtga gatgtgggcg
     4261 caactgcgcg acgacgtgat gcgctacggc atttacaacc agaatcttca ggcggtgccg
     4321 ccaaccggtt ctatctctta tatcaaccat gctacgtcga gtattcatcc gattgtggcg
     4381 aaagtagaga tacgcaaaga gggcaaaaca ggacgcgttt actaccctgc cccgtttatg
     4441 actaacgaaa atctggcgct gtatcaggat gcttacgaaa tcggcgcaga aaagatcatc
     4501 gacacctacg cggaagcgac tcgccatgtc gatcagggtt tgtcgctgac gctgtttttc
     4561 cccgataccg ccaccactcg cgatatcaac aaagcgcaga tttacgcctg gcgcaaaggt
     4621 atcaaaacgc tctattacat tcgcctgcgt cagatggcgc tggaaggcac tgaaattgaa
     4681 ggctgcgtct cctgtgcact ttaaggaata tctatgaaac tctcacgtat cagcgccatc
     4741 aactggaaca agatatctga cgataaagat ctggaggtgt ggaatcgcct gaccagcaat
     4801 ttctggctac cagaaaaggt gccgctgtcg aacgatattc ctgcctggca gacattaact
     4861 gtcgtagaac aacaactgac gatgcgcgtt tttactggcc tgacgctgct cgacacgctg
     4921 caaaatgtta tcggcgcgcc ttcgctgatg cccgatgcac tcacgcctca tgaagaagcg
     4981 gtattatcga atatcagctt tatggaagcg gttcatgccc gctcttacag ttcgattttc
     5041 tcgacgctgt gccagaccaa agatgtcgat gccgcctacg cctggagtga agaaaacgca
     5101 ccgttgcagc gaaaagctca gattattcag caacattatc gcggtgatga tccgctgaaa
     5161 aagaaaatcg ccagtgtgtt tcttgaatct tttttgttct attccggttt ctggctgccg
     5221 atgtattttt ccagccgcgg aaagctgacc aataccgcag acctgatccg tctgattatc
     5281 cgtgatgaag cagtccacgg ttactacata ggctataaat atcagaaaaa catggaaaag
     5341 atatctctgg gacaacgtga agagttgaag agtttcgcct tcgatttgtt gctggaactc
     5401 tacgacaacg agctgcaata caccgatgag ctgtacgccg aaaccccgtg ggctgacgat
     5461 gtgaaagcgt ttctctgtta caacgccaat aaggctttga tgaatctggg ctacgaacct
     5521 ttatttcccg cagaaatggc ggaagtgaat ccggcaatcc tcgccgcgct ttcgccgaat
     5581 gccgatgaaa atcacgattt cttttccggt tcaggctcct cttatgtgat ggggaaagcg
     5641 gttgaaacag aagatgaaga ctggaatttc tgagggtgtt attttcaaaa atatcactac
     5701 ccgcgacagg gaaataattc ccgccaaata gctttttatc acgcaaatat tttgtggtga
     5761 tctacactga tactctgttg cattattcgc ctgaaaccac aatattcagg cgttttttcg
     5821 ctatctttga caaaaaatat caacttttct cgatttgctc tcagccctta tatcacggga
     5881 aattccggcg atttgctcgc atcaatattc atgccacatt tgccatcagg ggttgcctca
     5941 gattctcagt atgttagggt agaaaaaagt aactatttcc attgggtaat atatcgacat
     6001 aaacaaataa caggaatctt tctattgcat ggcaattaaa ttagaaatta aaaaccttta
     6061 taaaatattt ggtgagcatc cacagcgagc attcaaatat atcgaacaag gactttcaaa
     6121 agaacaaatt ctggaaaaaa ctgggctatc gcttggcgta aaagacgcca gtctggccat
     6181 tgaagaaggc gagatatttg tcatcatggg attatccggc tcgggtaaat ccacaatggt
     6241 acgccttctc aatcgcctga ttgaacccac ccgcgggcaa gtgctgattg atggtgtgga
     6301 tattgccaaa atatccgatg ccaaactccg tgaggtgcgc agaaaaaaga ttgcgatggt
     6361 cttccagtcc tttgccttaa tgccgcatat gaccgtgctg gacaatactg cgttcggtat
     6421 ggaattggcc ggaattaatg ccgaagaacg ccgggaaaaa gcccttgatg cactgcgtca
     6481 ggtcgggctg gaaaattatg cccacagcta cccggatgaa ctctctggcg ggatgcgtca
     6541 acgtgtggga ttagcccgcg cgttagcgat taatccggat attttattaa tggacgaagc
     6601 cttctcggcg ctcgatccat taattcgcac cgagatgcag gatgagctgg taaaattaca
     6661 ggcgaaacat cagcgcacca ttgtctttat ttcccacgat ctcgatgaag ccatgcgtat
     6721 tggcgaccga attgccatta tgcaaaatgg tgaagtggta caggtcggca caccggatga
     6781 aattctcaat aatccggcga atgattatgt ccgtaccttc ttccgtggcg ttgatattag
     6841 tcaggtattc agtgcgaaag atattgcccg ccggacaccg aatggcttaa ttcgtaaaac
     6901 ccctggcttc ggcccacgtt cggcactgaa attattgcag gatgaagatc gcgaatatgg
     6961 ctacgttatc gaacgcggta ataagtttgt cggcgcagtc tccatcgatt cgcttaaaac
     7021 cgcgttaacg cagcagcaag gtcttgatgc ggcgctgatt gatgcgccgt tagcagtcga
     7081 tgcacaaacg cctcttagcg agttgctctc tcatgtcgga caggcaccct gtgcggtgcc
     7141 cgtggtcgac gaggaccaac agtatgtcgg catcatttcg aaaggaatgc tgctgcgcgc
     7201 tttagatcgt gagggggtaa ataatggctg atcaaaataa tccgtgggat accacgccag
     7261 cggcggacag tgccgcgcaa tccgcagacg cctggggtac accgacgact gcaccgactg
     7321 acggcggtgg tgctgactgg ctgaccagta cgcctgcgcc aaacgtcgag cattttaata
     7381 ttctcgatcc gttccataaa acgctgatcc cgctcgacag ttgggtcact gaagggatcg
     7441 actgggttgt tacccatttc cgtcccgtct tccagggcgt gcgcgttccg gttgattata
     7501 tcctcaacag tttccagcaa ttgctgctgg gtatgccagc gccagtggcg attatcgttt
     7561 tcgctctcat cgcctggcag atttccgggg tcggaatggg cgtggcgacg ctggtttcgc
     7621 tgattgccat cggcgcaatc ggtgcctggt cgcaggcaat ggtgactctg gcgctggtgt
     7681 taaccgccct gctgttctgt atcgtcatcg gtttgccgtt ggggatatgg ctggcgagaa
     7741 gtccgcgagc ggcgaaaatt attcgtccac tgcttgatgc catgcagacc acgccagcgt
     7801 ttgtttatct ggtaccaatc gtcatgctgt ttggtatcgg taacgtgccg ggcgtagtgg
     7861 tgacgatcat ctttgctctg ccgccgatta tccgtctgac gattctgggg attaaccagg
     7921 ttccggcgga tctgattgaa gcctcgcgct cattcggtgc cagcccgcgc cagatgctgt
     7981 tcaaagttca gttaccgctg gcgatgccga ccattatggc gggcgttaac cagacgctga
     8041 tgctggctct ttctatggtg gtcatcgcct cgatgattgc cgtcggcggg ctgggtcaga
     8101 tggtacttcg cggtatcggt cgtctggata tgggccttgc caccgttggc ggcgtcggga
     8161 ttgtgatcct cgccattatc ctcgaccgcc tgacgcaggc cgttgggcgc gactcacgca
     8221 gtcgcggcaa ccgtcgctgg tacaccactg gccctgtcgg tctgctgacc cgcccattca
     8281 ttaagtaact ctgcacttgc ccggtaacgc cgggcattat caccctgcca aaaaaaggaa
     8341 taacaatgcg acatagcgta ctttttgcga cagcgtttgc cacgcttatc tctacacaaa
     8401 cttttgcagc cgatctgccg ggcaaaggca ttactgttaa tccagttcag agcaccatca
     8461 ctgaagaaac cttccagacg ctgctggtca gtcgtgcact ggagaaatta ggttataccg
     8521 tcaataaacc cagcgaagtg gattacaacg ttggctacac ctcacttgct tccggcgatg
     8581 caaccttcac cgccgtgaac tggacgccgc tgcatgacaa catgtacgaa gctgccggtg
     8641 gcgataagaa attttattgt gaaggggtat tcgttaacgg cgcggcacag ggttacctga
     8701 tcgataagaa aaccgccgac cagtacaaaa tcaccaacat cgcacaactg aaagatccga
     8761 agatcgccaa actgttcgat accaacggcg acggtaaagc ggatttaacc ggctgtaacc
     8821 caggctgggg ctgcgaaggt gcgatcaacc accagcttgc cgcgtatgaa ctgaccaaca
     8881 ccgtgacgca taatcagggg aactacgcag cgatgatggc cgacaccatc agtcgctaca
     8941 aagagggcaa accggtgttt tattacacct ggacgccgta ctgggtgagt aatgagctga
     9001 agccagggaa agatgtggtc tggttgcagg tgccgttctc cgcactgccg ggcgataaaa
     9061 acgccgatac caaactgccg aatggtgcga attatggctt cccggtcagc accatgcata
     9121 tcgttgccaa caaagcctgg gccgagaaaa acccggcagc agcgaaactt tttgccatta
     9181 tgcagttgcc agtggcagat attaacgccc agaacgccat tatgcatgac ggcaaagcct
     9241 cagaaggcga tattcagggc catgttgatg gttggatcaa agcccaccag cagcagttcg
     9301 atggctgggt gaatgaggcg ctggcagcgc agaagtaatt tttattcggg cggataagtc
     9361 gtttttgcct catccgccat tctgtgcaca atgcctgatg cgacgctggc gcgtcttatc
     9421 atgcctacaa aatacactca ttccgtatgg cggataaggc gttttcgccg catccgccgt
     9481 tctgtgcaca atgcctgatg cgacgctggc gcgtcttatc atgcctacaa accacatcgc
     9541 acatttcaac aagctatctt tcgcccccat tcatcaacat ccgctattat tgatttccag
     9601 cttaatcatc acctgatgaa caaaaataat gactaaacct aatcatgagc ttagcccggc
     9661 gctgatcgtg ctgatgtcta tcgccaccgg tcaggcggta gccagtaact attacgccca
     9721 gccattgctc gacaccatcg cgcgtaactt ttccctttcc gccagttcgg caggctttat
     9781 tgttaccgcc gcgcagttgg gctatgcagc aggtctgctg tttcttgttc ccctcggtga
     9841 tatgtttgaa cgccgccgcc tgattgtctc gatgacctta ctggcggcag gcggcatgtt
     9901 aatcactgcc agcagtcagt cgctggcgat gatgatcctc ggtacggcat taaccggttt
     9961 attctcagtc gtggcacaaa ttctggttcc gctggcagcg acgctggctt caccggataa
    10021 acgcggcaaa gtggttggca ccattatgag cggtctgctg ttggggatct tgctggcacg
    10081 aaccgttgcc gggttactgg caaacctcgg cggctggcgc accgtctttt gggttgcctc
    10141 ggtgttaatg gcactgatgg cgctagcatt atggcgtggt ctgccacaaa tgaaatcaga
    10201 aacccacctc aactacccac agttgttagg ttccgttttc agcatgttta tcagcgataa
    10261 gatcctccgc acccgcgcat tgctgggctg cctgaccttt gccaacttca gcattctctg
    10321 gacctcaatg gcctttttgc ttgccgctcc accttttaac tacagcgatg gtgtcattgg
    10381 tctgtttgga cttgcgggag ctgccggagc gttgggcgct cgtccggcgg gcggttttgc
    10441 cgataagggt aaatcgcacc acaccacaac tttcggtctg ctgctgctat tactttcatg
    10501 gctggcgatc tggtttggtc acacttccgt actggcgctg attatcggca tcctggtgct
    10561 ggatctcacc gtacagggcg tgcatatcac taaccagacg gtaatttatc gaatacaccc
    10621 tgatgcgcgt aatcgcctga ccgcaggtta catgaccagc tactttattg gcggtgccgc
    10681 cggttcgcta atttcagcct cagcctggca acatggcggt tgggctggcg tttgtctggc
    10741 tggcgcgacg attgccctag ttaacttact ggtctggtgg cgaggttttc atcgtcagga
    10801 agccgcaaat taagcaatca gcaaccgttt tcgtggcttt acacttataa gggtgttaag
    10861 aagcccatca gtctgataag gttaagatat tcattcagtc tatttataat attaacaatc
    10921 gttaagcgta cactctatgg aaagccctac tccacagcct gctcctggtt cggcgacctt
    10981 catggaagga tgcaaagaca gtttaccgat tgttattagt tatattccgg tggcctttgc
    11041 gttcggtctg aatgcgaccc gtctgggatt ctctcctctc gaaagcgttt ttttctcctg
    11101 catcatttat gcaggcgcga gccagttcgt cattaccgcg atgctggcag ccgggagtag
    11161 tttgtgggtt gctgcactga ccgtcatggc aatggatgtt cgccatgtgt tgtatggccc
    11221 gtcactgcgt agccgtatta ttcagcgtct gcaaaaatcg aaaaccgccc tgtgggcgtt
    11281 tggcctgacg gatgaggttt ttgccgccgc aacagcaaaa ctggtacgca ataatcgccg
    11341 ctggagcgag aactggatga tcggcattgc cttcagttca tggtcatcgt gggtatttgg
    11401 tacggtaata ggggcattct ccggcagcgg cttgctgcaa ggttatcccg ccgttgaagc
    11461 agcattaggt tttatgcttc cggcactctt tatgagtttc ctgctcgcct ctttccagcg
    11521 caaacaatct ctttgcgtta ccgcagcgtt agttggtgcc cttgcaggcg taacgctatt
    11581 ttctattccc gtcgccattc tggcaggcat tgtctgtggc tgcctcactg cgttaatcca
    11641 ggcattctgg caaggagcgc ccgatgagct atgaggttct gctgcttggg ttactggttg
    11701 gcgcggcgaa ttattgcttc cgctatttgc cgctgcgcct gcgtgtgggt aatgcccgcc
    11761 caaccaaacg tggcgcggta ggtattttgc tcgacaccat tggcatcgcc tcgatatgcg
    11821 ctctgctggt tgtctctacc gcaccagaag tgatgcacga tacacgccgt ttcgtgccca
    11881 cgctggtcgg cttcgcggta ctgggtgcca gtttctataa aacacgcagc attatcatcc
    11941 caacactgct tagtgcgctg gcctatgggc tcgcctggaa agtgatggcg attatataac
    12001 ccacaagaat catttttcta aaacaataca tttactttat ttgtcactgt cgttactata
    12061 tcggctgaaa ttaatgaggt catacccaaa tggatagttc gtttacgccc attgaacaaa
    12121 tgctaaaatt tcgcgccagc cgccacgaag attttcctta tcaggagatc cttctgactc
    12181 gtctttgcat gcacatgcaa agcaagctgc tggagaaccg caataaaatg ctgaaggctc
    12241 aggggattaa cgagacgttg tttatggcgt tgattacgct ggagtctcag gaaaaccaca
    12301 gtattcagcc ttctgaatta agttgtgctc ttggatcatc ccgtaccaac gcgacgcgta
    12361 ttgccgatga actggaaaaa cgcggttgga tcgaacgtcg tgaaagcgat aacgatcgcc
    12421 gctgcctgca tctgcaatta acggaaaaag gtcacgagtt tttgcgcgag gttttaccac
    12481 cgcagcataa ctgcctgcat caactctggt ccgcgctcag cacaacagaa aaagatcagc
    12541 tcgagcaaat cacccgcaaa ttgctctccc gtctcgacca gatggaacaa gacggtgtgg
    12601 ttctcgaagc gatgagctaa cgcgtcatct cgctcaaaaa tccagattta taaaagaaaa
    12661 aatgaccggc cagcatcgca acatgctggc ctttttggca agcaggtcgg ctcagccgat
    12721 gagttaagaa gatcgtggag aacaatatga gcgcaaatgc ggagactcaa accccgcagc
    12781 aaccggtaaa gaagagcggc aaacgtaagc gtctgctcct ccttctcacc ttgctcttta
    12841 taattattgc cgtagcgata gggatttatt gttttttggt actgcgtcat ttcgaagaaa
    12901 ccgatgacgc atacgtggca gggaatcaga ttcaaattat gtctcaggtg tctggcagcg
    12961 tgacgaaagt ctgggccgat aacaccgatt ttgtaaaaga aggcgacgtg ctggtcactc
    13021 tcgacccgac agatgctcgc caggcgtttg aaaaagccaa aactgcactg gcttccagcg
    13081 ttcgccaaac ccaccagctg atgattaaca gcaaacagtt gcaggcgaat attgaggtgc
    13141 agaaaatcgc cctcgcaaaa gcacaaagcg actacaaccg ccgtgtgccg ctgggcaatg
    13201 ccaacctgat tggtcgcgaa gagctgcaac acgcccgcga cgccgtcacc agtgcccagg
    13261 cgcaactgga cgtcgctatt caacaataca atgccaatca ggcgatgatt ctggggacta
    13321 aactggaaga tcagcctgcc gtgcaacagg ctgccaccga agtacgtaac gcctggctgg
    13381 cgctggagcg tacccgtatt gtcagtccga tgaccggtta tgtctcccgc cgcgcggtac
    13441 agcctggggc gcaaattagc ccaacgacgc cgctgatggc ggtcgttcca gccaccaata
    13501 tgtgggtgga tgccaacttt aaagagacgc agattgccaa tatgcgtatc ggtcagccgg
    13561 tcactatcac cacggatatt tacggcgatg atgtgaaata caccggtaaa gtggttggtc
    13621 tggatatggg cacaggtagc gcattctcac tgcttccggc gcaaaatgcg accggtaact
    13681 ggatcaaagt cgttcagcgt ctacctgtgc gtatcgaact ggaccagaaa cagctggagc
    13741 aatacccgct gcgcattggt ttgtccacac tggtgagcgt caataccact aaccgtgacg
    13801 gtcaggtact ggcaaataaa gtacgttcaa caccggtcgc ggtaagtacc gcgcgtgaaa
    13861 tcagcctggc acctgtcaat aaactgatcg acgatatcgt aaaagctaac gctggctaat
    13921 ccagaggtgc gtgtgatgca acagcaaaaa ccgctggaag gcgcgcaact ggtcattatg
    13981 acgattgcgc tgtcactggc gacattcatg caggtgctgg actccaccat tgctaacgtg
    14041 gcgatcccca ctatcgccgg gaatctgggc tcatcgctca gccagggaac gtgggtaatc
    14101 acttctttcg gggtggcgaa tgccatctcg atcccgctta ccggctggct ggcaaagcgc
    14161 gtcggggaag tgaaactgtt cctttggtcc accatcgcct ttgctattgc gtcgtgggcg
    14221 tgtggtgtct ccagcagcct gaatatgctg atcttcttcc gcgtgattca ggggattgtc
    14281 gccgggccgt tgatcccgct ttcgcaaagt ctattgctga ataactaccc gccagccaaa
    14341 cgctcgatca cgctggcgtt gtggtcgatg acggtgattg tcgcgccaat ttgcggcccg
    14401 atcctcggcg gttatatcag cgataattac cactggggct ggatattctt catcaacgtg
    14461 ccgattggcg tggcggtggt gttgatgaca ctgcaaactc tgcgcggacg tgaaacccgc
    14521 accgaacggc ggcggattga tgccgtgggg ctggcactgc tggttattgg tatcggcagc
    14581 ctgcagatta tgctcgaccg cggtaaagag ctggactggt tttcatcaca ggaaattatc
    14641 atccttaccg tggtggcggt ggtggctatc tgcttcctga ttgtctggga gctgaccgac
    14701 gataacccga tagtcgatct gtcgttgttt aagtcgcgca acttcaccat cggctgcttg
    14761 tgtatcagcc tcgcgtatat gctctacttc ggcgctattg ttctgctgcc gcagttgttg
    14821 caggaggtct acggttacac ggcgacctgg gcaggtttgg cctctgcgcc ggtagggatt
    14881 attccggtga tcctgtcgcc gattatcggc cgcttcgcgc ataaactgga tatgcggcgg
    14941 ctggtaacct tcagctttat tatgtatgcc gtctgcttct actggcgtgc ctataccttt
    15001 gaaccaggta tggattttgg cgcgtcggcc tggccgcagt ttatccaggg gtttgcggtg
    15061 gcctgcttct ttatgccgct gaccaccatt acgctgtctg gtttgccacc ggaacgactg
    15121 gcggcggcat cgagcctctc taactttacg cgaacgctgg cggggtctat cggcacgtcg
    15181 ataaccacga ccatgtggac caaccgcgag tcgatgcacc atgcgcagtt gactgagtcg
    15241 gtaaacccgt tcaacccgaa tgcccaggcg atgtacagtc aactggaagg gcttgggatg
    15301 acgcaacagc aggcatcagg ctggattgcc cagcagatca ccaatcaggg gctgattatt
    15361 tccgccaatg agatcttctg gatgtcagcc gggatattcc tcgtactgct ggggctggtg
    15421 tggtttgcta aaccgccatt tggcgcaggt ggcggtggcg gcggtgcgca ctaagtacaa
    15481 ctaagccagt tcatttgaac tggctttttt caattaattg tgaagatagt ttactgacta
    15541 gatgtgcagt tcctgcaact tctctttcgg cagtgccagt tcttcgttgc tgttgatgcg
    15601 tacgtcacgt tccagaatgc tacgcgcaat atcctgcgct tcctgcaacg agtgcatctg
    15661 gtaagtgcca cactggtaga cgttcagttc cgggatctga ttctgatcct gcactttcag
    15721 cacgtcttcc attgccgctt tccaggcatc agcaacacgc tgctcatctg gcgtaccaat
    15781 cagactcata taaaaaccgg tgcggcagcc cattggcgag atatcgataa tctctacacc
    15841 attaccgtta agatggttac gcataaaacc agcaaacagg tgctccaggg tatggatccc
    15901 tctttctggc atcacttctt tgttcggcac gcagaagcgc agatcgaaca cggtgattgc
    15961 gtcgccatgc ggggtgttca ttgttttcgc cacccgaact gcaggcgctt ccatccgggt
    16021 atgatcgact gtgaagctat ctaacaacgg catttagcca cctccggtaa tttttttaaa
    16081 aattttctga actctttctt cccaggcgag tctgagtata tgaaagacgc gcatttgtta
    16141 tcatcatccc tgaattcaga gatgaaattt tggccactca cgagtggcct ttttcttttc
    16201 tgtcaggtgt gtttttccag ccacaccgca aacggttcgg tatcagcggt ttccatttcc
    16261 tgctgacggc gttcagatgc ctcgcgctcg gctacaaaat cctcttcgcg cagaatttcc
    16321 agcggctctt cacgcagcag attacggtag gcttctgcaa acgctttgcc tgttccgcca
    16381 ataccagtat caatcataga ccttaagata cgggcagaga aagtcagatc gggattatcg
    16441 aagcaggcaa ccagttcatc acacactttc tgatacgcgt cgccgccgtt aatgctatcc
    16501 agcgtttgcg cgacgcgttt cagatcgcgg aacagatctt tacccacctg cagtaacggg
    16561 aactgcgcgg tttcgcagcc gatacccagc gtcaggcccg gtttgcgacc ttcaaggatc
    16621 acccggttcc agttaacgcg cgtacaggca agttcgctac tgctcatttc cggtgcatca
    16681 gccagcgcac accagaccat aaacaggtcg aggaatcgca cctgctgttc atctacacca
    16741 atcggcgaga acgggttgat gtccagcgaa cgcacttcaa tatattcaat gccgccacgt
    16801 aacagcgcat cagaaggtga ctcgccgctg cgggtaacgc gttttggacg aatcggcgca
    16861 tacagttcgt tttcaatctg cagcacgttg ctattgattt gcagcctctt accgtctttc
    16921 tcaataccaa tcttcgcgta ctcttccgat ggcgttttga ttgcctgttt aaggcccgct
    16981 acgtactcgt aaagatcgtt gaaggtaata ccaagattgc tttgcgattt attggtatag
    17041 ccgagatcgc tcaaacgaag agaggtcgca tacggcaggt aatacatacc gcactcggtt
    17101 ttctcaaacg gcagcgacgt tggttttcct tgcaggaaag aagaacaaat cgccggagat
    17161 gcaccaaaca gataaggaat gacccaaccg aaacgatagt aattgcggat aacgcggaaa
    17221 tagcccgcag aaattttctc tttggcatca gcgcccgaga tatcaccgca cttcgcttgc
    17281 cagaatgcca ttggcaaaga gaaattgtag tgcacgccgg aaatggtttg catcagcgcg
    17341 ccgtagcgat ttttcagccc ttcacgatac agcgttttaa agcgtccggt gttagaagtg
    17401 ccgtactgtg ccagttcgat gtcctgacct tctgcgatgt agcatggcat acttaacggc
    17461 cacatccgct catcgcccat attgcgcgcc gtataacgat gcagatcgcg cataaaggtc
    17521 agcatatgtt caatatcacc atccactggt gtaatgaatt ccagcaatgc ttccgcaaaa
    17581 tcggtagtaa tccatttgtg cgtcagtgcg gaacctaatg cttcaggatg acctgttgtc
    17641 gccagtgtgc catcagcatt aacacgcaaa gtttcgcgct ccagcccacg ctgtatcccc
    17701 tttaacgcct gaggatgttt ttccagccag gccagcgcct gtgatacgtc cgggatcaaa
    17761 ttgacctccc gcctgtcaaa atcgttttaa ttagcataac tgtaatggtg accatatgtg
    17821 caggcctaca attagtgcca ccacatcatg ccctgaacgg tcgctgctgc aactgcaaca
    17881 tagcgtaacg ctttaccaag gcacaaaaaa aagattaccg gtccccacga gatgcgcatc
    17941 catcccgcta acagacacag taaatcgcca accacgggca tccagcttaa taatagcgtg
    18001 actgcaccat agcgtttcag ccagccgata gctttctctt gccagcgcga tgttttacgc
    18061 aatggaaaga aacgcccaag gataacgtta gttaaccctc caaggctatt acccattgtt
    18121 gctgttaaaa ctaaaaccca gggatgactg atcccggaaa gcaacattgc caccagcacg
    18181 acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac tactggcaaa caacgagaaa
    18241 agcgataacg cttcactcac agcaagcgaa cgtccacagc gtccatacct gcggcacggg
    18301 ccgcctgaat accgaaatcg gcatcttcaa agaccacaca ctgcgtcggt tgcacgccca
    18361 tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt atggtgtttg acgtgatcgg
    18421 cagcgacgac ggcgtcaaaa taatggcgta atcccaggtg cgccagcaat gcctcagcga
    18481 tggcgctttc actccccgtt cctacagcca ttgggcgacg accatgccaa cttttcacca
    18541 catcaacaag aggaagcggt tcgacgctat ccagcagcat acttcttact gcttctgttt
    18601 tttcacgcgc taacgcatgc gggtcgagat cggcctgatt cagctcaata attgcctgag
    18661 caatacgcca ggtgggcgat ccattaagcg caatcatcgc ctgaatatcg tactgaagac
    18721 cgtagtgccc taatacttcg cgccacgctt tacggtgcgt aggctccgta tccaggattg
    18781 tgccatccat atcaaaaatt aaacctgcat aacgctcgta catggtcttc tcgcaagtcg
    18841 aaaaatatga cgctacttta gcgtaattgc ttgattttgt cgctgatggc gaaggagaag
    18901 aaaattcagg aaatggataa aggagttatt tagaaatgag atatttttga aggaaatttt
    18961 ttggagaaga tggtgcatcc gggaggatga ctcactgacg ttcgcccttc gggccgttgc
    19021 tggcgcaacg ttatccttcc tggtgcctgc ggttaatacc gctatctctt cgataccttc
    19081 attgctgaag gttacggaga atatggtgca tccgggagga ttcgaacctc cgaccgctcg
    19141 gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaaa cttattctac
    19201 tgctgatatt gataccgcta ctaaagccat atcaattaag agatggtgca tccgggagga
    19261 ttactcgcct tgcggctcgc ccttcgggcc gttgctaaag caacgttatc atccctggtg
    19321 cctgcggtta attccgctac ctcttcgata ccttcattgc tgaagatttc ggagaa
//
LOCUS       DAQSWQ010000079        19372 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.079, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000079 DAQSWQ010000000
VERSION     DAQSWQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.079"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="R8G18_002913"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="R8G18_002913"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEE9796549.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="R8G18_002914"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="R8G18_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEE9796550.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="R8G18_002915"
     CDS             complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="R8G18_002915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEE9796551.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="R8G18_002916"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="R8G18_002916"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEE9796552.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="R8G18_002917"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="R8G18_002917"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEE9796553.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="R8G18_002918"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="R8G18_002918"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEE9796554.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="R8G18_002919"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="R8G18_002919"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEE9796555.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="R8G18_002920"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="R8G18_002920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEE9796556.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="R8G18_002921"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="R8G18_002921"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEE9796557.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="R8G18_002922"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="R8G18_002922"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEE9796558.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="R8G18_002923"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="R8G18_002923"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEE9796559.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="R8G18_002924"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="R8G18_002924"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEE9796560.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="R8G18_002925"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="R8G18_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEE9796561.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="R8G18_002926"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="R8G18_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HEE9796562.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="R8G18_002927"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="R8G18_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HEE9796563.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="R8G18_002928"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="R8G18_002928"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEE9796564.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="R8G18_002929"
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="R8G18_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HEE9796565.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="R8G18_002930"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="R8G18_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HEE9796566.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         4632 a         5123 c         4849 g         4768 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat acaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cttggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagagctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggctca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat agccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gcgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccgaccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgtggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atggagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcgt tagaggtcag cccttccggt tcaaggaaga tctgatgctg atttctgtcg
    12961 gcgaagcgca tgactttgtc ttcgatcgat gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcgt acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    13201 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacgaccacc gctgtaatta
    13321 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactaccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgattatgg cttgatggta cgcatgatcg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    14881 agtggcctgt tgtgtaatag gcttcagtga cttcagcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttatt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtttgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    15961 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gtgtatggcc agctgctgtt cgcgagaacg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttggg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttcagcgag
    16741 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca gaagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgca gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcttgaact tcactcaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggtgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt caccaggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgaac gacaagatgt tgggcgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttaccg cgaatttcac cacctttgct cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gtagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt
    18361 tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gg
//
LOCUS       DAQSWQ010000080        18843 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.080, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000080 DAQSWQ010000000
VERSION     DAQSWQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.080"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="R8G18_002931"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="R8G18_002931"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEE9796567.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2587)
                     /gene="hupB"
                     /locus_tag="R8G18_002932"
     CDS             complement(2315..2587)
                     /gene="hupB"
                     /locus_tag="R8G18_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HEE9796568.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="R8G18_002933"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="R8G18_002933"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEE9796569.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="R8G18_002934"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="R8G18_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HEE9796570.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="R8G18_002935"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="R8G18_002935"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEE9796571.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="R8G18_002936"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="R8G18_002936"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEE9796572.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="R8G18_002937"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="R8G18_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HEE9796573.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="R8G18_002938"
     CDS             9871..10449
                     /locus_tag="R8G18_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEE9796574.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="R8G18_002939"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="R8G18_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HEE9796575.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12430..13377
                     /gene="cyoA"
                     /locus_tag="R8G18_002940"
     CDS             12430..13377
                     /gene="cyoA"
                     /locus_tag="R8G18_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HEE9796576.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13399..15390
                     /gene="cyoB"
                     /locus_tag="R8G18_002941"
     CDS             13399..15390
                     /gene="cyoB"
                     /locus_tag="R8G18_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEE9796577.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15380..15994
                     /locus_tag="R8G18_002942"
     CDS             15380..15994
                     /locus_tag="R8G18_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEE9796578.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15994..16323
                     /locus_tag="R8G18_002943"
     CDS             15994..16323
                     /locus_tag="R8G18_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEE9796579.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16335..17225
                     /gene="cyoE"
                     /locus_tag="R8G18_002944"
     CDS             16335..17225
                     /gene="cyoE"
                     /locus_tag="R8G18_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEE9796580.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17410..17721
                     /locus_tag="R8G18_002945"
     CDS             17410..17721
                     /locus_tag="R8G18_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEE9796581.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17705..18247
                     /locus_tag="R8G18_002946"
     CDS             17705..18247
                     /locus_tag="R8G18_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEE9796582.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18303..>18843)
                     /locus_tag="R8G18_002947"
     CDS             complement(18303..>18843)
                     /locus_tag="R8G18_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEE9796583.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4214 a         4956 c         4581 g         5092 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 ctcgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctaaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttccccccca cccaattgtc accataatga taattatttg
    12181 ttaaacaatt gttttattcc agattggtta taccaattgc ccgtccaaac tccttctgca
    12241 ctcccctttc gttataacgc ccttttgcaa cagcttctta aaatcaactt gatatgtttt
    12301 gcaacatatg taacctgaca gccaaatcca agtaacagga atttaatcat gtttacagta
    12361 atgtaacctc cccgtaaaat gcccacacac tttaaacgcc accagatccc gtggaattga
    12421 ggtcgttaaa tgagactcag gaaatacaat aaaagtttgg gatggttgtc attatttgca
    12481 ggcactgtat tgctcagtgg ctgtaattct gcgctgttag atcccaaagg acagattggt
    12541 ctggagcaac gttcactgat actgacggca tttggcctga tgttgattgt cgttattccc
    12601 gcaatcttga tggctgttgg tttcgcctgg aagtatcgtg cgagcaataa agatgctaag
    12661 tacagcccga actggtcaca ctccaataaa gtggaagctg tggtctggac ggtacctatc
    12721 ttaatcatca tcttccttgc ggtactgacc tggaaaacca ctcacgctct tgagcctagc
    12781 aagccgctgg cacacgacga gaagcccatt accatcgaag tggtttccat ggactggaaa
    12841 tggttcttca tctacccgga acagggcatt gcaaccgtga atgaaatcgc tttcccggcg
    12901 aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt cttcattccg
    12961 cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca tctgatcgcc
    13021 aacgaacccg gtacttatga cggtatctcc gccagctaca gcgggccggg cttctcaggc
    13081 atgaagttca aagctattgc aacaccggat cgcgccgaat tcgaccaatg ggtcgcaaaa
    13141 gcgaaacagt cgccgaacac catgtctgac atggctgcgt tcgaaaaact ggccgcgcct
    13201 agcgaataca accaggtgga atatttctcc aacgtgaaac cagacttgtt tgccgatgtg
    13261 attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga aggtgagcac
    13321 agtgcacacg aaggtatgga aggcatggac atgagccacg cggaatccgc ccattaaagg
    13381 ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc gttccatgaa
    13441 cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct cgttggcctg
    13501 atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc cgtcgaccat
    13561 aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg tggttttgct
    13621 gacgccatta tgatgcgtag ccagcaggct cttgcctcgg cgggcgaagc gggcttcctg
    13681 ccacctcacc actacgatca gatcttcacc gcgcacggcg tgattatgat cttcttcgtg
    13741 gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc cgctgcagat cggcgcgcgt
    13801 gacgttgcgt tcccgttcct caacaactta agcttctggt ttactgttgt tggtgtgatt
    13861 ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct ggcctatcca
    13921 ccgctatcgg gaatagagta cagtccggga gtcggtgtcg attactggat atggagtctc
    13981 cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac cattctgaag
    14041 atgcgcgcac cgggcatgac catgttcaag atgccagtat ttacctgggc atcactgtgc
    14101 gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc gttgttgacc
    14161 ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa catgatgatg
    14221 tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat cctgcctgtt
    14281 ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt tggttatacc
    14341 tcgctggtat gggcaaccgt ctgtatcacc gtgctgtcgt tcatcgtttg gctgcaccac
    14401 ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac cacaatgatt
    14461 atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta tcagggccgc
    14521 atcgtgttcc attctgcgat gctgtggacc atcggtttta tcgtcacttt ctcggtgggc
    14581 gggatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca taacagcctg
    14641 ttcctgattg cgcacttcca taacgttatc atcggcggcg tggtcttcgg ctgcttcgca
    14701 gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaatgaaac ctggggtaaa
    14761 cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccgct gtatgcgctg
    14821 ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt ccacaccatg
    14881 ctgatgattg cagctagcgg tgcagtactg attgcgctgg gtattctctg cctcgttatt
    14941 cagatgtacg tttctattcg cgaccgcgac cagaaccgtg acctgactgg cgacccgtgg
    15001 ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa ctttgccgtt
    15061 gtgccgcacg ttcacgagcg tgatgcattc tgggaaatga aagagaaagg cgaagcgtat
    15121 aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc aggtatcgtc
    15181 attgcagctt tctccaccat cttcggtttc gccatgatct ggcatatctg gtggctggcg
    15241 attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga cgaggacgtg
    15301 gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt cgatgagatt
    15361 actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg cccacgcgca
    15421 cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga tctacctgat
    15481 gagcgactgc attctgttct caatcttgtt tgctacctat gccgttctgg tgaacggcac
    15541 cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg ttgaaacttt
    15601 cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt acaaaaacaa
    15661 caaaagccag gttatctcct ggctggcgtt gacctggttg tttggtgccg gatttatcgg
    15721 gatggaaatc tacgaattcc atcacctgat tgttaacggc atgggtccgg atcgcagcgg
    15781 cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca cttccggtct
    15841 tatctggatg gcggtgctga tggtacaaat cgctcgtcgc ggcctgacca gcactaaccg
    15901 tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt ggatctgtgt
    15961 gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga tcacagcggc
    16021 gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat cattctgacg
    16081 gtgattccgt tctggatggt gatgacagga gctgcctctc cggccgtaat tctgggaaca
    16141 atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct gcacatgaat
    16201 accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct aatcatcgct
    16261 atcctggttg taggctccat ctggattatg tggaacctca actacaacat gatgatgcac
    16321 taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca ggcatcatct
    16381 ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc agcattgatt
    16441 atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg ggttgtgtgt
    16501 ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag aatcgggtgc
    16561 tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg ctaggctttg
    16621 ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg ggggtgatgg
    16681 gctttgtggt ttatgtcggc gtttatagcc tgtacatgaa acgccactcg gtctacggca
    16741 cattgattgg ttcgctctcc ggcgctgcgc cgccggtgat tggctactgt gcagtaaccg
    16801 gcgagttcga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg cagatgcctc
    16861 actcctatgc catcgccatt ttccgcttta aggattatca ggcggcaaac attccggtat
    16921 tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat atcatcgcct
    16981 ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tgggtataaa tatctggtgg
    17041 tcgccgcggc ggtgagcgtc tggtggttag gtatggccct gcgcggttat aaagttgctg
    17101 atgacaggat ctgggcgcgc aagctgttcg gcttctctat catcgccatc acagccctct
    17161 cggtgatgat gtccgttgat tttatggtgc cggactcgca tacgctgctg gctgctgttt
    17221 ggtaacacaa cctctctatt aaaaaggtgc tacggcacct tttttattgt ctgagatcag
    17281 agatacggct aataaccgta tgtcttgaca gagtgagcag atgaacataa attaatcata
    17341 cggttgatag ccgtattatg tatgagatcg actccctacg atttcattca gttaatcaaa
    17401 gaggtcataa tgcctggaaa aacagccact ctacccgatg tcgataaaac gctgaaaaat
    17461 gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca aacttcgtga agctgcgcaa
    17521 gccgttgggg tagatttaag cgcttttatt ttgagcgctg ctatggaacg cgccgaaagt
    17581 gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa gctgggaact gatgaaccaa
    17641 ctcatcgctg aacctgctca accgacgctc gccctcaagg cgttaatgaa aaggaaaaac
    17701 agcgatggtc gacaagcatg aagagattac tctgcccata gtcctctcct gtaattatca
    17761 gtctgatatt acttatcctg ggcaaaaaca gtttgattgc ggtaactctg ttatcgataa
    17821 atttgtacgc gcatcgctaa agaaaagtgt gcgtaatagc gactgtgcgg ctaaagcact
    17881 tattgacaga caaagtggtg aactgatcgg catctgtact tttacggcat attcgctgga
    17941 aaaacaacgc gtttctggcg tccttcaggg ttcacaacct tcagaaattg gtgttgtcag
    18001 attagtcatg ttgggggtag cacggaagta tcaaaagcgg ggctttggtc aggacctact
    18061 atgtgatttt tttgaacatg taaaaataat tcaccaggca ttaccaatta aaggggttta
    18121 tcttgatgct gaccctgccg ccattaattt ttatgctcgt ctcggctttg ttcagctttc
    18181 agcgacacca aatgcttttg gtgctgtacc tatgtttttg gcgattcagc atattctcgc
    18241 ggcttagatt attatcgcca cccgccaggg caagttttgc cttagccggg ggctgagcag
    18301 cgttaccact tttgttcaat tgcccccgct ttcagcaact ccacatcctt gccgttaatg
    18361 gttgccgttg cggcaacgat tgtggtcctt acagttgcac aagattttcg ctcaaattcc
    18421 ggtttataaa gaataagaga cgaattactg gtaaatgaac caaataggta atcagtaaag
    18481 ctaatattca gccggtttaa accgaaaggc gcgaagccaa ccgtttggtt aatctcctta
    18541 ccggatgggc gacgaatgct gaatgtcacg cgaagattgg ccacttcgcc taatttattg
    18601 gtggtggcga agaggctatc catgcaggaa aaacctttat ctaaaaaatt atctcgcatc
    18661 ccagaacggg taaccgtaat cacgggtaac gtatcgtcaa ccgtttttct gtcaatcatg
    18721 tcttttcgac aagtagccag aaagtcttcc gggccaccat aaatgtggac acctttatca
    18781 agatattcct tcgctttctt acaatctacc tcaacatact gaccattggc gtacataatg
    18841 ccc
//
LOCUS       DAQSWQ010000081        18824 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.081, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000081 DAQSWQ010000000
VERSION     DAQSWQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18824
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.081"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            39..1499
                     /locus_tag="R8G18_002948"
     CDS             39..1499
                     /locus_tag="R8G18_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEE9796584.1"
                     /translation="MVNGTFIYWLNLNTPGNLRGITTRCVLLDEVSSCEITDEGNPIK
                     LAEARTSTFGSDSLVVVSSTPLYKDDLINAEYNLSDKRRYFVTHTCGHEYTFEWEQVA
                     FEFKQLENGRSIPDSTTTRLLCPHCNEGIDEHTRHQMVDNGRWIATNPDGEPGVVGYQ
                     ISRMYSPLNTITEMVSKFADALYNFNLQTFYNNELGLPFEDEYQKELDILQLESLRED
                     EFNLYKIPESTLALCISVDQQGDRLESLLLGFDEKNIYVLGHEFFYSHDCTKIEAPAW
                     KDLDQFCRQDFSTVSGRIVPTLAVFIDAGNGNATDTVKKFTTRWSKYHPIKGSSSTTG
                     ELFKTSTQAGYKLQILNVHEQKNTIRRLLNLMLSSEADNAPVKLRFSSTLPSDFFEQL
                     SAEELKPAGGKLVWRLKKGQRRNEALDLICYGMIAIAYAQSKLGTQPFRKLRDYKAQE
                     TTKYEINKVEEPKPETVQKPKRNRRTGMGSNWFGKR"
     gene            1511..2041
                     /locus_tag="R8G18_002949"
     CDS             1511..2041
                     /locus_tag="R8G18_002949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796585.1"
                     /translation="MTLLPEKIYMVSNPMDLTVLVPAATILVISFTSSGKSTSLDNLN
                     GSTSKEFTVVLDMSTAQELLFCTQISNGQTSTFTSEVINPLLYTSEYSQLKQMISEID
                     QVIANKIAGGANYSITINNKTLVSESLSSLESMRERYVKRANALFVKMNGGTFSNGGK
                     PIKSITVFRPKPGSTR"
     gene            2041..3549
                     /locus_tag="R8G18_002950"
     CDS             2041..3549
                     /locus_tag="R8G18_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEE9796586.1"
                     /translation="MFWNKKNKNSETKPKKIKQERSLKQSTLKRDLQAVRNTSVMNFG
                     FNSNSGSNINFLLMKALPTFRAFSRDAVLKNPIGRKYMNLSVDGVVGSDGLYVKPSVE
                     IDGTEEEINGINLTLEKLFDRWAYDADRFSVDGSLSFDLFQQNAEKVRVQDGECFIRI
                     HNINNTIKLEILDTVRLLQLNNQRLSNGNYISNGIEFDKWHKPVNYYFCKFDPVTYTY
                     ITGDYEVIPAAEICHYFIADQQGQERGLPDMVATSKLMEDLKNFTEAALTAKRVSASS
                     MAFITNNNDTTSTDLLGADEREEVTPVYTEYFEAGFIGELAEGQDIKTVTPTNGVDGI
                     DQFTNELMNQISMGLNITKQALLSDTSNASFSASRLTEKLQQTTFRTRTNVLISKVLK
                     PIYIAWLKNEMLNNNKLNLSFSSFDDLICARYISQKPISLDPVKDIQAELLQLEAGIK
                     SKTQVIAELGGDPVKVLAEVQAEKDKENKNKEVIQDGNEKPEEGTNETPTGD"
     gene            3503..5341
                     /locus_tag="R8G18_002951"
     CDS             3503..5341
                     /locus_tag="R8G18_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEE9796587.1"
                     /translation="MKNQKRELTKPLQAINTESRTIEVAFCSETPVEREINGQIYNEI
                     LLCGSSNADLTRLNNNGAVLFNHNRDDLIGAVVDARVDADRVGRATLRISSTANDEWE
                     MIQEGVLTHISIGYNINDYQIDGNNIYVTNYEIYEISLVTVPADPTVGVGRSMDSQLA
                     LKSLNKEGETIQEGQMDMDNPENEKESIVDSTEEVNETIEEALVEPSVEYAEEIKQEL
                     SDEELLEIISTRPDLLNKVHGEEPESINSTEDTERVRELEALGKVLNIDVSEAIETGI
                     SISDFKRQLNDIKQNPLHDKEIKEMDNKNLLKDMVRAIKTGDKSALEAYERGINGFVR
                     AAVPSTNTTTAAGVVADDLQDQYIPELLKISALGELNTTVYSGLAGRGTLSIPKAAGV
                     SPVFKFYGEAEAQDDSIASFTKVTLSPKAFGGAIPLSKQAILTAPNIESFVQSELLRY
                     AAQGLEQNVMDKVVAAAPVFNVETAGSITLEDVQGAVAKLAQANVDMRGVKAVMNAKT
                     LSTLRQIAVLDNTAAKAMVEGYRSTEMWLADEVRVVISEFVADGTILMGDFSNVILAN
                     WEGQEVDYDDTSYRSSNTIVYRVWDYSDIALAHSEAFVNIVIGKAE"
     gene            5355..5678
                     /locus_tag="R8G18_002952"
     CDS             5355..5678
                     /locus_tag="R8G18_002952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796588.1"
                     /translation="MRVFKDTQCDAFLNAFGQSIQTCTGSTFTGIVEVLPVSIEAAGG
                     FIESVETYLTIRKDDLITANVAIGTVLIIDGVNQTIYNIEDDLSGMVNCYFRTSAGAS
                     FAEDY"
     gene            5680..6072
                     /locus_tag="R8G18_002953"
     CDS             5680..6072
                     /locus_tag="R8G18_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796589.1"
                     /translation="MMLVQKVRNTMKQLINATKNLTVSRDVDVFEQIAFDYSLTSLSF
                     SNERRAGNFAIQYLISPKPESGNTAPSITYDQIISTFDKEKTSAFSDAGLIMLSCSYE
                     QSDIVTDPITGSVSLSFTINIQVTEKTR"
     gene            6090..8741
                     /locus_tag="R8G18_002954"
     CDS             6090..8741
                     /locus_tag="R8G18_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEE9796590.1"
                     /translation="MSDIFSGRELSVQYNADIGNRTPQGAGNVVIKQINTFPTLTINS
                     EANNFETYDSDYRTVLLSDKSVAPFDIVVNYLPDDPTHQFLDNAAKSQQLFQIIIQYQ
                     MNKDENTITYAMVNGYITSYKLDGDKGSVVTKAYSFAPHDVIARAMTINALSPIYQGD
                     YGLGSNTTDVPQYSPSVPTGNSFIKVPSSQAGNPAGADLMGVGLVDGTTVSSLAMTKT
                     GTLSIYAKNATTAWTRIYTATQMDGRYVPLTRTINGHALTANLVLNSEDTGSVPDTRT
                     VNGKPLSEDVVLTSTDTGSVPDTRTVNGHVLSEDIVLSSEDTGSVPDTRTVNGHVLSE
                     NVVLSSEDTGSVPDTRMVNGHVLSEDVVLTSTDVGLGKVTDDAQLTIAGNLSDLGDVE
                     EARINLAVYSMDEVDAEVTALADSITSLDSASMKKASNLSDLSSLSTARTNLQVNRLT
                     QGATYTDLMSNDLTNRIRIQDNGAWGASTGTGWIALGIGQGGTGATDALSARLNFQVD
                     RFNQGSNLTTIKSADSSAALVVYDGGQWGSATYIAGAFTGWKALAIPQGGTGVKTQAE
                     LWPAIRPTGATSLAGDPVSDSDATTKRWVENLLSSRTSVSPQWDVIASSPSSTAIGSY
                     VTGGRIRSICNIGTSTEISGGFRVGKTMGQTGQADLTLFISENGAEDKLWGLKGPTGT
                     ILTDVQSGWMTNTAVSGPTDANTITFNGMYAGPGDSGINYSEKYSPLLHMQRYPGANR
                     SQLQIDGGGTLRVRGGNGTTWFGWLVMQAQGTSGREFKRDITPANPVEALDRIMSQDL
                     VTFIYKDDEQERVRFGVIAEDAELTAPQYIKHDEETVEIEGEIVTRDHPRVDLNPIVM
                     DLMGAIQAQQEEINQLKDLVQQLLNNK"
     gene            8800..9273
                     /locus_tag="R8G18_002955"
     CDS             8800..9273
                     /locus_tag="R8G18_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796591.1"
                     /translation="MAMDIFSGANLTVEVGSSAGKTVATDFVEVPEVNTFSTSGFEST
                     VISVKTFNNAYDRKLLGTKSIPDISLAVNYLPDNAVHQNLEQLADDQKRCQVKLSYFT
                     DATKKEGFFVIYTCFVSSTTIGGDKDEVVTKTFTLAVDGEAIDSGLITPKPESGE"
     gene            9325..9624
                     /locus_tag="R8G18_002956"
     CDS             9325..9624
                     /locus_tag="R8G18_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796592.1"
                     /translation="MNLNELKKKLQPELKPHEVMGEIIFIHRPNGQDFQKCTDVAKTL
                     ILCVKDENGDPIFAEEDIDGRINVNSIDFVIQNEIYSAIIALINTAKPVDEVEKK"
     gene            9726..10019
                     /locus_tag="R8G18_002957"
     CDS             9726..10019
                     /locus_tag="R8G18_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796593.1"
                     /translation="MIYDTFIEPSGTQIEMMKHAYQCYYTTISNGNLTPEARKSIKVQ
                     DFDFLDVLSDSTKSTSEKAEERKLKTKEAQSNDIKSIGELIKSQVLGKKKNGK"
     gene            10009..12222
                     /locus_tag="R8G18_002958"
     CDS             10009..12222
                     /locus_tag="R8G18_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796594.1"
                     /translation="MANNNRIRVDITGDSSGLQRALRSGTESLEEFGSTAGGIVEEFT
                     GRITGMAGGFGTAMTGIAGAAAIGIGGLAALVESSREYVREMNQISKSTGLSVVQLQQ
                     LSAAFSGLGLEIDKFGDFNKDTLDKLGDAFRAGGGVSDDLKEYGLNLQDYNKYLKQAD
                     GGMQAVIHTFYAMRDAGKSQSEIVNVMETLASDSSHMISTLQQFNNEAQATAYIQSQN
                     AEVTNDAAEKYAEFDKNLGKLTTSIKGTIADGLSPLVNAMNSVYDAANQKPHEAGLFE
                     DLNERIKTSKGSLQDMLDIWEKLRTAGALNYQGAALNTGIMDDKGNKGNEFAQAKARL
                     EELANNLKSDVAVATAPTGGWVDQAKEAEDAAKKLEQQRKQAEAAQKAADAKRLQAQR
                     NLEAALAQVGEDGFQVRLQQFDRQQKALLKTITDSAQVLGINPDEMLKNATAYGAKQR
                     TDLLNSMVGYQDPNQGLKDTNALIGSGLLNDNQKGYLAQQQNQRINGGNPLAYNDTDQ
                     KLKDNADAMNAELQQNELLLKGHEDYEKRKAEITAKYNAQAIQISNQNAQDQLSIFSS
                     TAQSLSQGMVDAFGESSGAAQAAFTLSKSISIAQTVLSIQSALAQALATPWPASLANY
                     AQVLSLGMSIISTAKGAAAGQFHGGVDELPASYDNKSFVLKQGERVVQPEANKKLTAF
                     LDKQEGGSTSGDITVNAPLIIQGDVADDDKKFNEMLKKHANSVSQAVRSSQKRNT"
     gene            complement(12233..12805)
                     /locus_tag="R8G18_002959"
     CDS             complement(12233..12805)
                     /locus_tag="R8G18_002959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796595.1"
                     /translation="MLPRISSFFIYLKTSIRIFFNRLDWQAASVIVAMLLFAGQIGYN
                     AYDKWKESSDDVFKMRVILLSEINNNLLALATGGDESQVLGTYEICIPLPTEDTEAIK
                     RVATAVSYLHDDVYYSQLEKLASLPKKEVDAIVKSYYSLSRLRTLSTQLAYNIKYSPP
                     KVKLLREEYYNLYTLSSSLSQYLSYHTENP"
     gene            12807..13364
                     /locus_tag="R8G18_002960"
     CDS             12807..13364
                     /locus_tag="R8G18_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796596.1"
                     /translation="MASFSNQIKVTNFQIKSTEPIYSNQTWTGQRIMRSTGIQYYQIQ
                     FTLNFNPASLNEVNSFLAQYAQGKPFTMSLGLAGTYYGKQTGAVTATALTKPGNMVVP
                     ISTNTLAVGEWVQFSNHNKLYRIVERTSTSITIFPALQNTVQASEALKYNNLMIEAVL
                     DPDNDYSLPIGNIMNITLKATENII"
     gene            13364..13987
                     /locus_tag="R8G18_002961"
     CDS             13364..13987
                     /locus_tag="R8G18_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEE9796597.1"
                     /translation="MDSGIYTNANLLKYWKLVRGTTKTRLTLMEIMSLGVNVTCFDVL
                     PKGTNGFHWTDSLIDIDLDGYKYISFPDIISGSLPSYSEQKGMSNDAINFKISNVNAS
                     VRALALGGFLKDAQMNIKLVILNPYDSTVIDSMLMFTGFIDYVQAVTDPNQKQNEMTI
                     YVNSVYKKLDRQPALIAANSVYQSYYRNDEYFSLLGQVNQNQNWKYK"
     gene            13972..14391
                     /locus_tag="R8G18_002962"
     CDS             13972..14391
                     /locus_tag="R8G18_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEE9796598.1"
                     /translation="MEIQVMKNLHNEVMSIIQYSIDNPYKYGDNDCNIIVLRLIDLIN
                     GTTQLTNRQYSSIKEGIAGLNKEGWNHTGEIVEAYCKPVQATIDGDIWLDPDNPLIMA
                     VVASGRVLGVNADHDGFELQPKPTEGTYYRVRKQRDG"
     gene            14384..17686
                     /locus_tag="R8G18_002963"
     CDS             14384..17686
                     /locus_tag="R8G18_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796599.1"
                     /translation="MGKSLGGFFGAIITAVVVAAAVYFSGGTALAAIGWGAAAGSLSL
                     VASSMLGQIGVSGYGDVADSLSRSTSPTTGLPVIYGGELPHKNGVSGGSFILTGSIVA
                     WYNVPNSDSQYLFSEQAVAYAGVEKHIEQIYIDNEPVLAVPITAEGVVPKESIAAKYQ
                     NYLQLEVRFGGDYTSTKTLAAKYAGSKWTNKFLGKGIVSISTVILKTQDSLENNILVN
                     DQFALTVEMKGQVIYDFVDGTYKASSNPPSIIYDYLTNSIYGMGIDPSLINTDTFAET
                     AAYCDAFEYYANGAISYQKTYKDNIENICQSFGGIMYVHAGQICITTDRKSVSVASFD
                     ERNMVGAVQVSTSGGTDYFNVVDCKFTNPESMYTTDVVRIPSDITTDEAVQHDGQVIA
                     LSRDYSWSYDQEVIAKMANVDVLKAKYALRTISFTTSEGWDLEVWDAINVSNEELAIS
                     GKFKVLNKDISTDQENVGYVTITAVEAPDAMYDGIDPGIWSPGGVINFPELQVLPPTN
                     LQAVRKGNTTSGSIVDLTWDASPDPYLRGYYVYYRLNGSSTWTYAGQTGVQKLDYELF
                     GLSDTASYDFSVEAYNNLGLVSKKLSLTGIVPTFNFALPAVTRVVLTNKTESAYVTDS
                     PDFNIAWDSQKDIRVNNRSFSEYFKYYVIKIYDGTKLVDTFYTQSNAFNFTLEMNRLK
                     VRKPTIGIIAQGFNDGTYSEEVKITVENKQCGLVEDVSFTGGFGNLFASWTKSTERDY
                     AGAIISIVNGTNTRIFTSYAPEFDSIPNITDGEYKVKMGFFDVFGTDDIQYCPEQTIS
                     INSKYQFTEDDAEAINGILDLEDRLTGTINDAVKIANKNTSTVISASEARTNDKITAS
                     EKTLSTQITGVNSSLSQKLSTVESTANGNKASIQTLSQTVTDNNKAQTTAVSQLRSDV
                     NGQIATVNQSMSTKADKSTVDAQYSLSVQANGTVAGMRLVASSGTSNNSAIYFAANKF
                     IVSGTDTATVGGTAPFAIVNGTTYLKTAMIQAASIGTGYIADAAITNLKLASGSVNTL
                     NVVDGCITTAKISNTIQSTNYVANKTGWQINKAGTILINGTGGTGRMVISNNIIQIYD
                     NNNTLRVRMGLW"
     gene            17844..18140
                     /locus_tag="R8G18_002964"
     CDS             17844..18140
                     /locus_tag="R8G18_002964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796600.1"
                     /translation="MAQGLQCWNAAGVLVVDLTDYNIRFMGTYTVKATSAQTYTVSVP
                     NMKTTGWFVHYAPASDTFNEWSAFCNNGSFTAVYLPTYAPLAGNYTFNVYKWTA"
     gene            18142..18798
                     /locus_tag="R8G18_002965"
     CDS             18142..18798
                     /locus_tag="R8G18_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796601.1"
                     /translation="MAGFEVYNSAGALTIDSTNKSIMTGAVKAMGNLTDTGYYTGFTC
                     AFGNGGSLGFVMPSVVANRNTTQYWFQIQKDGAWCFPGAYMFQPGMGRFMTSSHTATP
                     TSGFLDVFSEEGTLIWSAASAATMPRIRGFLTAPAATDLSTAITVTSPVADPWFCWSQ
                     CPGNISDDGTVIGYSGLVIRRNSSTSFSLQYVSKNQKTYRQAMGNNGIQIALATFTGY
                     "
BASE COUNT         6057 a         3465 c         4063 g         5239 t
ORIGIN      
        1 atgataagaa cgccgccaat aactctaaac aaacacaaat ggtgaacggt acttttatct
       61 attggttgaa cctcaacacg ccggggaacc ttcgcggtat cacaacacgc tgtgttctgt
      121 tagatgaggt cagtagctgc gaaatcactg atgaagggaa cccaatcaaa ctggcagaag
      181 cacgtaccag taccttcggt agtgactcac tggtggttgt atcttccacg ccattgtata
      241 aagacgattt aatcaatgct gagtacaacc tcagtgataa acgtaggtac tttgttactc
      301 acacatgcgg tcatgaatat acttttgaat gggaacaagt agcgtttgaa tttaagcaat
      361 tagaaaatgg tagatccatt cctgatagta ctactactcg tttgttatgc cctcattgca
      421 atgagggaat agatgaacat acacggcatc agatggttga taatggtcga tggattgcaa
      481 ctaatccaga tggggaaccg ggtgtagtag ggtatcaaat cagtagaatg tattcacctc
      541 tgaatactat tactgaaatg gtttcaaagt ttgctgatgc tctatataac ttcaatcttc
      601 aaacctttta caataacgaa ttgggcttgc cattcgaaga tgagtatcaa aaagaattag
      661 atattctcca attagaatcc ctacgagaag atgaatttaa cctttataaa attcctgaaa
      721 gtaccttagc gttgtgtatt tcagttgatc agcagggtga taggctggaa agtctgcttt
      781 taggttttga tgaaaagaac atttatgtac ttgggcatga gtttttctac tcccacgatt
      841 gtacgaagat tgaagcccca gcttggaagg atttagatca gttttgccgt caggatttca
      901 gtactgtatc aggtcgcata gtacctacac tggctgtgtt cattgatgca gggaacggta
      961 acgctacgga tactgtgaaa aaattcacta cacgttggag taagtatcat ccgatcaagg
     1021 gtagcagctc cactactggt gaactattca agaccagtac tcaggcgggg tacaaactac
     1081 agatcctaaa cgtacatgaa cagaagaaca cgatacgccg cctcctgaac ctgatgctat
     1141 ccagtgaggc agataacgcc ccggttaagc tccgcttcag tagtacctta ccatcagatt
     1201 tctttgagca actttcagca gaggagttaa aacccgctgg tggtaagttg gtatggcgat
     1261 taaagaaagg tcaaagaaga aatgaggctt tagacctaat ctgttatgga atgatcgcca
     1321 tagcatatgc ccaatcaaaa ttaggtactc aaccattcag gaagttacgc gattataaag
     1381 cacaggaaac cactaaatac gagataaata aagtagaaga accaaaacca gaaacagtac
     1441 aaaaaccaaa acggaataga cgtactggaa tgggatcaaa ctggttcggt aaaagataag
     1501 gatatcaaaa atgacacttc taccagaaaa gatctacatg gtatcgaatc ccatggatct
     1561 aacggtatta gtacccgctg ctactattct tgttattagt tttacatcat ctggaaaaag
     1621 tacctcatta gataatttaa atggtagtac ttcaaaagaa tttacggttg tattagatat
     1681 gagtacagca caggaattac tattctgtac tcaaatatct aatggtcaaa ccagtacttt
     1741 tacttctgaa gtcattaatc cattacttta tacttctgag tactcccaat taaaacagat
     1801 gatcagtgaa attgatcagg ttatagctaa taaaattgca ggcggtgcta attactcaat
     1861 aacaattaat aataagactc ttgtaagtga atcattatca tcgcttgaat caatgcggga
     1921 acgctatgta aaacgtgcta atgctctatt tgtgaaaatg aatggcggta ctttctctaa
     1981 tggcggtaaa ccaatcaaga gtattacggt tttcaggcct aagccaggga gtacacgcta
     2041 atgttctgga ataagaaaaa taaaaattca gaaactaaac ccaagaagat taaacaggaa
     2101 cgttccttaa aacaaagtac cttaaaacga gatctacagg cagtacgcaa cacatcagta
     2161 atgaacttcg gattcaattc aaactcaggc agcaatatca acttccttct gatgaaagca
     2221 ctacctacct tcagggcttt ctcacgtgat gcagtactta agaatcctat tggtcgtaag
     2281 tacatgaacc tatcggtaga tggcgttgta ggctctgatg gcctgtacgt taaaccttca
     2341 gtagagattg acggtactga agaagaaatc aacggaatca atctaacctt ggaaaagctc
     2401 tttgaccgtt gggcatatga tgctgatcgt tttagtgttg atggctcatt aagttttgac
     2461 ctgtttcagc agaacgcaga gaaggtaagg gtccaggatg gtgaatgctt tattcgtata
     2521 cacaatatta ataataccat caagttagaa attctcgata cggttcgttt gcttcagtta
     2581 aataatcaac gtttgagtaa tggtaattat attagtaatg gtattgaatt tgataaatgg
     2641 cataagccag ttaattacta tttctgtaaa ttcgatcctg ttacctatac ttatatcact
     2701 ggcgattatg aagtaattcc cgctgcggaa atctgtcact actttattgc tgatcaacaa
     2761 ggccaggaac gcggattgcc ggacatggtt gcaacttcca aattaatgga agatctcaag
     2821 aacttcactg aggctgcgtt aactgcgaaa cgtgtatctg catcgtcaat ggcttttatt
     2881 accaataata acgacactac cagtactgat ctgttagggg ctgatgaacg agaagaagtt
     2941 acaccagtat atacagagta ttttgaagct ggctttattg gtgagttagc cgaagggcag
     3001 gatattaaaa ctgttacacc tactaatggt gttgatggga ttgatcaatt taccaatgaa
     3061 ttgatgaatc agatctcgat gggtctgaac ataacaaagc aagccttatt atctgataca
     3121 tccaatgctt catttagtgc aagccgactt actgagaaat tacaacaaac tacattccgt
     3181 acccgtacta atgtactaat cagtaaagta cttaaaccaa tttatatcgc ctggttaaag
     3241 aacgaaatgc taaataacaa taagttaaat ctaagtttct cgagctttga tgatcttatt
     3301 tgtgctcggt atatttctca aaagccaatt agtttagatc ctgtaaagga tatacaggct
     3361 gaactactcc agttagaagc aggtattaaa tctaaaacac aggttattgc tgagttaggc
     3421 ggtgatccag tgaaggtatt agcagaggta caggcagaaa aggataaaga aaataaaaac
     3481 aaggaagtaa tccaggatgg aaatgaaaaa ccagaagagg gaactaacga aacccctaca
     3541 ggcgattaat acagaatcgc gtaccatcga agtagcgttt tgttcagaaa cccctgtaga
     3601 acgtgaaatc aacggtcaaa tctataacga aattcttcta tgtggttcca gtaacgcaga
     3661 cctaacccgc cttaataaca atggggcagt actctttaac cataaccgtg atgatcttat
     3721 tggtgctgta gttgatgcac gagtggatgc tgatcgcgtt ggtagagcta cattacgaat
     3781 tagcagtacc gccaacgatg aatgggaaat gattcaggaa ggcgtactaa cccatattag
     3841 cattggctat aacattaatg attatcaaat tgatggtaat aatatctatg ttaccaacta
     3901 cgaaatttat gaaatctcat tagtaacagt acctgccgat cctaccgttg gggtaggtcg
     3961 ttcaatggac tcacaacttg cattgaaatc actaaataaa gaaggtgaaa caattcaaga
     4021 gggtcaaatg gatatggata atccagaaaa cgaaaaggaa tcaattgttg atagtactga
     4081 agaagtaaat gaaacaattg aagaggcatt agtagagcca tcggtcgaat atgcagaaga
     4141 aatcaaacaa gaattaagtg atgaagaact tttagaaatt atctctactc gacctgattt
     4201 gctcaataaa gtacatggtg aagaaccaga aagtataaat agtacagaag atactgaacg
     4261 tgttcgtgaa ttagaagcac tcggtaaagt acttaatatt gatgtatctg aagcaattga
     4321 aacaggaatt tcaatttcag atttcaaacg tcaattgaat gacattaaac aaaatcctct
     4381 tcatgataag gaaatcaaag aaatggataa taaaaatcta ttaaaagata tggtacgtgc
     4441 cattaaaact ggcgataaat ctgctcttga agcatatgaa cgtggtatta atggttttgt
     4501 acgtgcggct gtaccaagta ctaatactac taccgctgct ggcgttgtag ctgatgacct
     4561 acaagatcaa tacattccag aactactaaa aatttctgca cttggtgaac taaacaccac
     4621 tgtttattct ggccttgcag gtcgcggtac tctaagtatt ccaaaagcgg ctggtgtatc
     4681 tccggtattt aaattctatg gtgaagcaga agcacaggat gattctattg caagctttac
     4741 taaagtaacc ctaagcccta aagcttttgg tggtgctatt ccactatcca aacaagcaat
     4801 tctaacggct ccaaatattg aatcatttgt acaaagtgaa ctactacggt atgccgctca
     4861 gggtcttgag caaaatgtaa tggataaagt agttgccgct gctccggtgt tcaatgttga
     4921 aactgctggt tctattactc ttgaagatgt tcagggtgca gtagcgaagc tggcacaggc
     4981 taacgttgat atgcgtggcg ttaaagctgt tatgaacgct aaaaccctaa gtaccctacg
     5041 tcagattgca gtacttgata acactgctgc taaagcaatg gttgaaggtt atcgcagtac
     5101 tgaaatgtgg cttgctgatg aagtacgtgt agtgatttct gaattcgtag cagatggtac
     5161 tattctaatg ggcgatttct ccaatgtaat tctcgctaat tgggaagggc aggaagtaga
     5221 ttatgacgat acatcctatc gttcaagtaa cactatcgta tatcgcgtat gggattactc
     5281 agatatcgca cttgcacact ctgaagcttt cgtaaatatc gttattggta aagcagaata
     5341 ataaggatta gataatgaga gtatttaaag atacgcagtg tgatgcgttt ctaaatgctt
     5401 tcggtcagtc tatccaaact tgtacgggaa gtacttttac aggaatagta gaagtacttc
     5461 ccgtttctat tgaggcggca ggtggattta tcgaaagcgt ggaaacatac ctaacaatca
     5521 gaaaggacga tctaataacc gcaaacgtgg caattggtac agtactaatc attgatggtg
     5581 taaaccagac aatctataac attgaagatg atctatcagg tatggttaat tgctatttcc
     5641 gtacttctgc tggtgcttca tttgcagagg actactaata tgatgttagt acaaaaagta
     5701 cggaatacga tgaagcagct tatcaatgct actaagaatc tgacagtatc acgtgatgtt
     5761 gatgtatttg aacaaattgc ttttgattac tcattaacga gtttaagttt cagcaatgaa
     5821 cgccgggcag gtaactttgc aattcagtat ctgatttccc ctaaaccaga atcaggtaat
     5881 acagccccat ccatcactta tgatcagatt attagtactt ttgataaaga gaaaacttca
     5941 gcattcagtg atgcaggttt aataatgctt tcgtgttctt atgaacaatc cgatattgtc
     6001 acagatccta taactggctc ggtttcccta tcctttacga taaatattca agttacggaa
     6061 aagacaagat aactaaaagg atataaagga tgagtgatat tttctcagga agagaattat
     6121 cagtacagta taatgctgat attggtaaca gaacaccaca gggagcaggc aatgtagtaa
     6181 ttaaacagat taatactttt ccaaccttaa caataaattc agaagcaaat aattttgaaa
     6241 cgtatgatag tgattacagg acagtactac tatctgataa atcagtagca ccatttgata
     6301 ttgtagttaa ctacctacct gatgatccta cacatcaatt cttggataat gccgctaagt
     6361 ctcagcagct tttccagatc atcattcagt accagatgaa taaggatgaa aataccatta
     6421 cttatgcgat ggtgaatggt tatatcacct cttataagtt ggacggtgat aaaggttcag
     6481 tagtaaccaa ggcatacagt tttgccccac atgatgtgat cgcacgtgca atgaccatta
     6541 acgcactatc gccaatctat cagggggact acggtttagg ttcgaacact acggatgtac
     6601 ctcagtactc accatcagta cctacgggta acagcttcat caaagtacca tcatctcaag
     6661 caggcaatcc tgccggggcg gatctgatgg gagtagggct tgtagatggc actactgtaa
     6721 gttctcttgc gatgacaaag acgggtactc taagtatcta tgcaaagaac gctacaaccg
     6781 cctggacacg catatatacc gctacacaga tggacgggcg atacgtaccc cttacacgta
     6841 ccatcaacgg ccatgcactc acggccaatt tagtactgaa cagtgaagat acaggttcag
     6901 tacctgatac acgtactgtg aatggtaagc ctctaagtga ggatgtagta cttaccagta
     6961 ctgataccgg ctctgtacca gatacacgga cagttaatgg tcatgtactc agtgaagata
     7021 tagtactgag tagtgaagat acaggttcag tacctgacac gcgtacagtg aacggtcatg
     7081 tacttagtga aaatgtagta ctgagtagtg aagatacagg ttcagtacct gatacacgta
     7141 tggtcaacgg tcatgtactc agtgaggatg tagtacttac cagtactgat gttggcttag
     7201 ggaaggttac agatgatgcc cagcttacga tagcaggtaa tctaagtgat ctgggggacg
     7261 tggaagaagc acgtattaac cttgctgttt actcaatgga tgaagtagat gcagaggtaa
     7321 cggctttagc tgacagtatt acttcccttg attccgcttc catgaagaaa gcgagtaacc
     7381 tttctgatct atccagtctc tctacagcac gtactaacct acaggtgaac cgtttgactc
     7441 agggtgctac gtacaccgat ctgatgagta atgacctgac taaccgtatc cgaattcagg
     7501 ataacggggc atggggtgca agtactggta ctggatggat tgctttaggt ataggtcagg
     7561 gggggacagg ggctacagat gctttgagtg ctcgcctaaa ctttcaggtg gatcgcttta
     7621 atcaaggtag taatttaaca actattaaat ccgctgattc ttccgctgca ttagtggttt
     7681 atgatggagg gcaatggggt tcagctacat atattgcggg tgctttcact ggctggaaag
     7741 cattagcaat tccgcagggc ggtactggtg ttaagactca ggctgagtta tggcctgcga
     7801 ttcgtcctac gggtgcaact tctttagcag gagatcctgt ctctgattct gacgcgacta
     7861 ccaaacgctg ggttgaaaac ttgctgagta gtcgtacttc agttagtcct caatgggatg
     7921 ttattgcttc ttccccttcc tctacggcaa ttggtagtta tgtaactgga gggcgtattc
     7981 gttcgatatg caacattggt acatcaactg aaatatctgg tggttttcgg gttggtaaaa
     8041 cgatgggaca gactggacag gctgatctca ccctttttat ttctgaaaat ggggcagagg
     8101 ataaactatg ggggctaaaa gggccgactg gaacgatcct aactgatgtt caatcaggat
     8161 ggatgactaa taccgctgtt tcgggaccga cagatgccaa taccatcacc tttaacggta
     8221 tgtatgcagg gccaggtgac tcaggtatta actatagtga aaaatattcc cctttattgc
     8281 acatgcaaag atacccaggg gcaaatcgct ctcagcttca gatagacggt ggtggtactt
     8341 taagagtacg aggcgggaac ggtacaacat ggtttggatg gttagtaatg caagctcaag
     8401 gtacatcagg tcgtgagttt aaaagagaca taactccagc aaatccagtt gaagcattag
     8461 atcggataat gtcacaagat ttagtaacgt ttatctacaa ggatgatgaa caagaaaggg
     8521 ttaggtttgg tgttatcgcc gaagatgctg agttgactgc tccgcagtac attaagcatg
     8581 atgaagaaac agttgaaata gaaggggaga ttgtcactcg tgatcatcca cgtgttgatt
     8641 taaatccaat agtgatggac ttgatgggag ctattcaagc ccaacaagaa gaaattaatc
     8701 agctaaaaga tttagtacaa cagctattaa ataataaata aatataaacg gggtgaacgg
     8761 attcactcca ataaaatttt cttataagga tataaaacta tggctatgga tattttttcc
     8821 ggtgctaacc ttactgtaga agtaggttca agtgctggta aaacagttgc tactgacttt
     8881 gtagaagtac cagaggtaaa caccttcagt acttcaggct ttgaaagtac tgtaattagc
     8941 gtaaaaacat tcaataatgc ttatgatcgt aagctattgg gtactaagtc aatcccggac
     9001 atttctcttg cagttaacta cttaccagat aatgcagtac atcagaattt agaacaactt
     9061 gcagacgatc agaaacgttg tcaggtaaaa ctaagctatt ttaccgatgc aactaaaaaa
     9121 gaaggtttct ttgtaattta tacctgcttt gtttcaagta ccactatcgg tggtgataaa
     9181 gatgaagtag taactaagac tttcaccctt gcagttgatg gtgaagcaat tgattcaggt
     9241 ctgattactc caaaaccaga atcgggcgaa taataataaa taaagggaag gtgctcaact
     9301 ttcccttttt tattggagat gaaaatgaat ttaaacgaat tgaagaaaaa actacaacca
     9361 gaattaaaac cacatgaagt aatgggtgaa atcatcttta ttcatcgtcc aaatggtcag
     9421 gatttccaga aatgcactga tgtggctaaa actctaatcc tatgtgtaaa agatgaaaat
     9481 ggtgatccta tcttcgcaga agaagatatt gatggacgaa ttaatgttaa ctctattgat
     9541 tttgtaatcc agaatgaaat ttactcagca attattgctt taatcaatac tgcgaaacct
     9601 gtagatgaag tagaaaaaaa gtaagaggtg atatcgaatt aaagtacttt tgtaagatgg
     9661 ttaacaaaag aggcttatca cctgaagaat actttagttt agatcctgat gtactcaata
     9721 tgcttatgat ctacgatact tttatagaac catccggtac gcagattgaa atgatgaaac
     9781 atgcgtacca gtgttactac accactatca gtaatggcaa cctaacacca gaagcaagga
     9841 aaagtatcaa ggtacaggat tttgattttc ttgatgtgct tagtgatagt actaagagta
     9901 cttcagagaa agctgaagaa agaaaattaa aaactaaaga ggctcaatcc aatgatatta
     9961 aatctatagg ggagttaatt aaatctcagg tattaggaaa gaaaaagaat ggcaaataat
    10021 aatagaatac gtgtagatat tacgggtgat tcttccggtt tacagcgtgc tttaaggtct
    10081 ggtactgaat cattagagga atttggtagt actgctggtg gcattgttga agagttcaca
    10141 ggccgaataa ctggtatggc tggcgggttc ggtactgcta tgaccgggat cgcgggtgct
    10201 gcggctattg gtattggtgg cctggcagca ttagtagaat catcacgtga atatgtgcgt
    10261 gaaatgaacc agatcagtaa gagtaccgga ttgtccgtgg ttcagttaca acagctatca
    10321 gcggcgttta gtggcctcgg tcttgagatt gataagtttg gtgatttcaa caaggatacc
    10381 ttggataagc tcggtgatgc ttttcgtgct ggcggtggtg tatccgatga tcttaaagaa
    10441 tatggactca acctacagga ctataacaag tacttaaaac aggctgatgg tggtatgcag
    10501 gctgtgatcc acacctttta cgccatgcgt gatgcaggta aatcacagag tgaaatagtt
    10561 aacgtaatgg aaacgttagc cagtgactca agccacatga ttagtactct tcagcagttc
    10621 aataatgagg ctcaagctac agcctatatc cagtcacaaa atgctgaagt aactaatgat
    10681 gctgctgaaa aatatgctga attcgataaa aaccttggga aacttactac cagcattaaa
    10741 ggtactattg ctgatggcct atcaccttta gttaatgcca tgaacagtgt ctatgatgct
    10801 gcaaatcaaa aacctcacga ggcaggtttg tttgaagatc tgaatgaacg tattaaaact
    10861 tccaagggtt ctctacagga tatgttggat atctgggaaa aattacgtac agcaggggca
    10921 ttaaactacc agggtgcagc actcaatact ggcatcatgg atgataaggg caacaagggt
    10981 aatgagttcg ctcaggctaa ggcacgttta gaagaactgg caaacaacct aaagtccgat
    11041 gttgcggttg ctaccgctcc tactggcgga tgggttgatc aggcaaaaga agctgaagat
    11101 gcagcgaaga agttagaaca acaacgtaaa caggcagaag cggcacaaaa ggcggcagat
    11161 gcaaaacgcc tacaggctca acgtaatctg gaagctgctc ttgctcaagt tggggaagat
    11221 ggttttcagg tacgtctaca gcagttcgac agacaacaaa aggcacttct taaaaccatc
    11281 accgatagtg ctcaagtact cggtatcaat cctgatgaaa tgctaaagaa cgctactgca
    11341 tatggtgcta aacaacgtac tgatttactg aattcaatgg ttggttatca agatcctaat
    11401 cagggtctga aagataccaa tgctcttatt ggttccggtc tactcaatga caatcagaag
    11461 gggtatctgg cacagcaaca gaatcagcgt atcaatggtg gtaatccatt agcctataac
    11521 gataccgatc agaagttgaa agataacgct gatgctatga atgctgaact tcagcagaat
    11581 gagttgttgc tgaaaggcca tgaggactat gagaaacgta aagctgagat cacggcaaag
    11641 tacaatgcac aggccatcca aatcagtaac cagaatgcac aggatcagtt aagtatcttc
    11701 tcaagtactg cacagtcact atcacagggc atggttgatg cgtttgggga aagtagtggg
    11761 gctgcacaag ctgcatttac cctaagtaag agcataagca ttgcacagac agtactttca
    11821 attcagtcag cattggccca agcactcgct accccttggc cagcttcatt ggcaaactat
    11881 gcacaagtac tttcacttgg tatgtcgatt attagtactg caaagggtgc tgctgctggt
    11941 cagttccatg gcggggtaga tgaactacca gcaagttatg acaacaaatc atttgtactt
    12001 aagcagggcg agagagtggt gcagcctgaa gcgaataaga aattaacagc attcttggat
    12061 aaacaagaag gtggaagtac ttcaggtgat attaccgtta acgcaccatt gatcatccag
    12121 ggtgatgtag ctgatgatga caagaaattt aatgagatgc ttaagaaaca cgccaatagt
    12181 gtttctcaag ctgtgagaag tagtcaaaaa cgtaatacat aagaaagccc agttagggat
    12241 tttccgtgtg ataagacaaa tattgtgaaa gagaagatga aagggtatag agattgtaat
    12301 actcttcccg caataattta actttaggtg gtgagtattt aatattgtat gcaagctgtg
    12361 ttgaaagagt acgtaatctc gataatgaat aataactctt tacgatggca tctacttctt
    12421 tttttgggag tgatgctaat ttttctaatt gggaataata tacatcatca tgcaaataag
    12481 aaacggcagt tgctactctt ttaatagctt ctgtatcttc agttggaaga ggaatacata
    12541 tttcatatgt gcctaacacc tgagactcat cgccaccagt agcaagagct aaaaggttat
    12601 tgttgatttc gctcaataag attacacgca tcttaaaaac atcatctgaa ctttctttcc
    12661 atttatcata tgcgttatag cctatttggc ctgcaaataa caacatagca acgatgacgg
    12721 atgcagcctg ccaatcaagc ctgttaaaaa aaatacgaat cgaggttttt aaataaataa
    12781 agaaagaaga tattcgaggt aacaaaatgg catccttttc aaatcaaata aaagtaacga
    12841 actttcaaat taaaagtact gaaccgatct attctaatca aacatggaca ggtcaacgta
    12901 tcatgagaag tactggaatt caatactatc aaattcagtt cacccttaat tttaatcctg
    12961 catcactaaa tgaagtgaat agctttttag ctcaatatgc acagggcaaa cctttcacta
    13021 tgtcattggg gcttgcaggt acatattacg gtaaacagac tggtgcagta accgctactg
    13081 ctctcactaa accgggtaat atggttgtac ctatcagtac taacacatta gctgtgggtg
    13141 agtgggtaca gttttccaat cacaataagc tatatcgaat cgtagaacgt accagtactt
    13201 caattactat tttccctgct ctacaaaata cagtacaggc aagtgaagca ctcaaatata
    13261 acaatctcat gatcgaggct gtattagatc cagacaatga ttattcattg cctattggga
    13321 acatcatgaa tattacattg aaagcaacgg agaatatcat ctaatggact caggtatata
    13381 tactaatgct aatcttctta agtactggaa gttagtcaga ggtacaacca agacaagatt
    13441 aacactaatg gaaattatga gcttaggcgt taatgttact tgttttgatg tactaccaaa
    13501 aggtactaat ggttttcact ggacggattc actaattgat atagatctgg atggttataa
    13561 gtacataagt ttccccgaca ttatcagtgg ttcattacct tcatattctg aacaaaaggg
    13621 catgtctaat gatgctatca attttaaaat tagcaatgta aacgcctcgg ttcgtgctct
    13681 tgcccttggt ggtttcttga aagatgcaca gatgaatatc aagttagtaa ttctcaatcc
    13741 atatgatagt actgtaattg attcaatgct catgtttacg gggtttattg actatgtaca
    13801 ggcagtaact gatccaaatc agaagcaaaa cgaaatgact atatatgtga attctgtcta
    13861 taagaaatta gacagacaac ctgcattaat tgccgccaac tcagtatatc aatcttacta
    13921 tagaaatgat gagtacttta gtctgttagg tcaagttaat caaaatcaga actggaaata
    13981 caagtaatga aaaatctaca taatgaagta atgagtatta ttcagtactc aattgataac
    14041 ccatataagt atggtgataa cgattgcaac attatagtac tacgccttat tgatttaatt
    14101 aatggtacta cacagttaac taatcgccag tattcgagca ttaaagaagg tatagcaggt
    14161 ttaaacaagg aaggatggaa ccatacaggg gaaattgttg aagcgtactg taagccagta
    14221 caagccacta tagacggtga tatctggtta gatcctgaca acccattaat tatggcagta
    14281 gtggcatctg gtcgggtact tggagtaaat gctgatcatg atggtttcga acttcaacca
    14341 aaaccaacag agggaacata ttacagagta aggaaacaga gagatgggta agagtttagg
    14401 gggctttttt ggagccataa ttacggcagt tgtcgtcgcg gctgctgtgt acttttcagg
    14461 aggtacagca ttagcagcaa taggttgggg tgcagcagcc ggatctttgt cattagttgc
    14521 aagttctatg ttaggtcaaa tcggggtttc tggatacggt gatgtagcag actcactaag
    14581 tagaagtact tcaccaacta ctggattacc cgttatatac ggtggagaac taccacataa
    14641 aaatggtgta tcaggcggtt ccttcatcct aaccggaagc atagtagcct ggtacaacgt
    14701 accaaattct gattctcagt acttattctc agaacaggct gtagcatatg ctggtgtaga
    14761 aaagcatatc gagcaaatct atattgataa tgagccagta cttgcagtac cgattacagc
    14821 agagggtgta gtacctaaag aaagtattgc agcgaagtac cagaactatt tgcaattaga
    14881 agtacgattt ggtggtgact atacgagtac taaaactctt gctgcaaaat atgctggttc
    14941 gaaatggaca aataaatttc ttggtaaggg tattgttagt atcagtaccg ttatcttaaa
    15001 aactcaagat tcattagaaa ataatattct tgttaatgat caattcgctt taactgtaga
    15061 aatgaaaggg caggtgattt atgattttgt tgatggtact tataaagcaa gtagtaaccc
    15121 tccttcaatt atctatgatt acttaacaaa ttcaatctat ggtatgggta ttgatccatc
    15181 attgattaat accgatacgt ttgcagaaac cgcagcatat tgtgatgctt ttgaatatta
    15241 tgctaatggt gctatcagtt atcaaaaaac ctataaagat aatattgaaa atatctgtca
    15301 gtcatttggc ggcatcatgt atgtacatgc cggacaaatc tgtattacca cagaccgcaa
    15361 atcggtatcc gtagcctcat ttgatgagcg taacatggta ggggcggtac aggtttctac
    15421 ttctggcggt actgactact tcaacgtagt cgattgcaag tttactaacc ctgaatcgat
    15481 gtacaccact gacgtagtac gtatcccttc cgatatcact acggatgaag cagtacagca
    15541 tgatggacag gtcatagcat tatcacgcga ttactcatgg tcatatgatc aagaggtgat
    15601 tgcaaaaatg gctaacgttg atgtactcaa ggctaagtat gccctgcgta ctatcagctt
    15661 caccacaagt gaaggttggg atctcgaagt atgggatgcg atcaatgtca gcaatgaaga
    15721 actcgctatc tcaggcaaat ttaaagtact caataaagat atcagcaccg atcaagagaa
    15781 cgttggctat gtaaccatca cggcagtaga agcacctgat gcaatgtatg atggtattga
    15841 tccgggtatc tggtcgcctg gtggggtaat taacttccct gaactacagg tactaccgcc
    15901 aacgaaccta caggcagtac gtaaggggaa tactacttct ggttcaatcg tggatcttac
    15961 ctgggatgct tcaccagatc cgtatctgcg tgggtactat gtttactacc gtcttaacgg
    16021 ttccagtacc tggacgtatg cggggcagac tggcgtacag aagcttgatt atgaattatt
    16081 tggattgtct gatactgcaa gctacgactt ttcggtagag gcttacaaca acttagggct
    16141 ggtatccaag aagctttccc taactggcat tgttccaaca ttcaactttg cactacctgc
    16201 ggtaacgcgt gtagtactga caaataaaac agaatcagcg tatgtaactg actcaccaga
    16261 tttcaatatt gcctgggatt cacaaaaaga tatcagagtg aataaccgat catttagtga
    16321 gtacttcaag tattacgtta ttaaaatcta tgacggtact aaattagtag atacattcta
    16381 tacgcaaagt aatgccttta atttcactct tgaaatgaac cgtctgaaag tacgtaagcc
    16441 taccattggt attatcgcac agggatttaa tgatggtaca tattcagagg aagtgaaaat
    16501 aaccgtagag aataaacagt gtggtttagt agaggatgtt agctttacgg gtggttttgg
    16561 taatttgttt gcttcatgga ctaaatctac tgaacgtgat tatgcaggtg cgattatcag
    16621 tattgttaat ggtactaata cacgtatatt caccagttat gcacctgaat ttgattcaat
    16681 accaaacatc actgatggtg agtataaggt taagatggga ttctttgatg tgtttggtac
    16741 tgatgatatt caatattgtc cagaacaaac aatcagtatc aactccaagt accagtttac
    16801 cgaagatgat gcagaggcaa ttaacgggat tcttgattta gaagatcgcc tgactggtac
    16861 cattaatgat gcggtcaaaa ttgccaataa gaataccagt actgttatca gtgcatcaga
    16921 ggcacgtacc aatgataaaa tcactgccag tgagaaaacg ttaagtactc agatcactgg
    16981 ggtaaatagt tccttatcac agaaacttag caccgttgaa agtactgcca atggtaacaa
    17041 ggccagtatc cagacactat cccagacggt aacggataac aacaaagctc agactacagc
    17101 agtctcacag ctacgtagtg atgtgaatgg acagattgcc actgttaacc agtcaatgag
    17161 tactaaagcc gataaatcta ctgtagatgc tcagtactca ctatcagtac aggctaatgg
    17221 cacggtagct ggtatgcgtc ttgttgcgag tagtggaacg agtaacaact cagcaatcta
    17281 tttcgctgcg aacaagttca tagtctccgg tactgacact gctactgtag gtggtactgc
    17341 accatttgcc atagtcaacg gtactacata cttgaagacg gcaatgatac aggcggcaag
    17401 tatcggtact ggttacatcg ctgatgcggc aattaccaat ctaaaactgg cctccggctc
    17461 agtgaataca ttaaacgtgg ttgatggttg tataactaca gccaaaatct caaatactat
    17521 tcaaagtact aattacgtag caaataaaac aggctggcaa atcaataaag caggtacgat
    17581 cctaatcaat ggaacgggtg gtactggtag aatggtaatc agtaataata ttattcaaat
    17641 ctatgacaat aataatacgt tacgtgtacg tatgggttta tggtaataaa taattcaggg
    17701 attattaact gtaacctcat ttcaaaagga tacataaatg aaatctaatg aacgtacttt
    17761 caatagtacc agtactaacc cattcagtat tagcggaaaa gaaacaattg gaatgaagaa
    17821 aaggatttac aggagtaaga gctatggcac agggattaca atgttggaat gcagcgggtg
    17881 tattagtagt agatctgact gattataaca tacgttttat gggtacttat actgtcaagg
    17941 ctacttctgc acagacctat actgtatcag taccaaatat gaaaactaca ggctggtttg
    18001 tacactatgc ccccgccagt gataccttta atgagtggtc agccttttgt aataatgggt
    18061 catttactgc ggtttatttg ccaacatatg caccgttagc tggtaactac acttttaacg
    18121 tatataagtg gacggcgtaa gatggcaggt ttcgaggtat ataattcagc gggtgcactg
    18181 actatagata gtactaacaa gtcgataatg acgggtgcag taaaggccat gggaaatcta
    18241 actgatacgg gatactacac aggctttacc tgtgccttcg gtaatggtgg atcgctcggt
    18301 ttcgttatgc catcagtagt agctaacagg aacacaacac agtactggtt ccagatacag
    18361 aaagacgggg catggtgttt ccctggtgcc tatatgttcc agcctggcat gggcaggttc
    18421 atgacaagta gtcacacggc tacccccaca tctggttttc ttgatgtgtt cagtgaagaa
    18481 ggtactttaa tctggtctgc tgcatcagcg gcaacaatgc cacgtatcag gggatttttg
    18541 acagcacctg cggctaccga tctcagtact gccattacgg taacgtctcc agtagctgat
    18601 ccctggttct gctggtcaca atgtccgggg aacatatcag atgatggtac agtaattggg
    18661 tactctggtc tggttatcag gcgtaattcc agtacttcat tcagtctaca gtacgtttca
    18721 aagaaccaga aaacctaccg ccaggcaatg gggaataatg gtatccagat agcattggcg
    18781 acatttacag ggtattaata caataacaat aacaataaca ataa
//
LOCUS       DAQSWQ010000082        18514 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.082, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000082 DAQSWQ010000000
VERSION     DAQSWQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.082"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="R8G18_002966"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="R8G18_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HEE9796602.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="R8G18_002967"
     CDS             735..1040
                     /locus_tag="R8G18_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796603.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="R8G18_002968"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="R8G18_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HEE9796604.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="R8G18_002969"
     CDS             1788..2534
                     /locus_tag="R8G18_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HEE9796605.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="R8G18_002970"
     CDS             2534..4630
                     /locus_tag="R8G18_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HEE9796606.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="R8G18_002971"
     CDS             4676..5815
                     /locus_tag="R8G18_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HEE9796607.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="R8G18_002972"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="R8G18_002972"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HEE9796608.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="R8G18_002973"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="R8G18_002973"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEE9796609.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="R8G18_002974"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="R8G18_002974"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HEE9796610.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="R8G18_002975"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="R8G18_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HEE9796611.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="R8G18_002976"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="R8G18_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEE9796612.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="R8G18_002977"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="R8G18_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HEE9796613.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="R8G18_002978"
     CDS             complement(12873..13730)
                     /locus_tag="R8G18_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HEE9796614.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="R8G18_002979"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="R8G18_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HEE9796615.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /locus_tag="R8G18_002980"
     CDS             complement(14122..14709)
                     /locus_tag="R8G18_002980"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HEE9796616.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="R8G18_002981"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="R8G18_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="R8G18_002982"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="R8G18_002982"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HEE9796617.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="R8G18_002983"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="R8G18_002983"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HEE9796618.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4546 a         4915 c         4782 g         4271 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataaca
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc agttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctaccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gcggccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggcg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaac
//
LOCUS       DAQSWQ010000083        18417 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.083, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000083 DAQSWQ010000000
VERSION     DAQSWQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.083"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(595..885)
                     /locus_tag="R8G18_002984"
     CDS             complement(595..885)
                     /locus_tag="R8G18_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796619.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            complement(885..1771)
                     /locus_tag="R8G18_002985"
                     /pseudo
     CDS             complement(885..1771)
                     /locus_tag="R8G18_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2135..2797)
                     /locus_tag="R8G18_002986"
     CDS             complement(2135..2797)
                     /locus_tag="R8G18_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HEE9796620.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            complement(3166..3717)
                     /locus_tag="R8G18_002987"
     CDS             complement(3166..3717)
                     /locus_tag="R8G18_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEE9796621.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            4304..4651
                     /locus_tag="R8G18_002988"
     CDS             4304..4651
                     /locus_tag="R8G18_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEE9796622.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            4704..5099
                     /locus_tag="R8G18_002989"
     CDS             4704..5099
                     /locus_tag="R8G18_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEE9796623.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            5140..5883
                     /gene="cmoA"
                     /locus_tag="R8G18_002990"
     CDS             5140..5883
                     /gene="cmoA"
                     /locus_tag="R8G18_002990"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HEE9796624.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            5880..6851
                     /gene="cmoB"
                     /locus_tag="R8G18_002991"
     CDS             5880..6851
                     /gene="cmoB"
                     /locus_tag="R8G18_002991"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HEE9796625.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(7016..9445)
                     /gene="torZ"
                     /locus_tag="R8G18_002992"
     CDS             complement(7016..9445)
                     /gene="torZ"
                     /locus_tag="R8G18_002992"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HEE9796626.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(9470..10570)
                     /locus_tag="R8G18_002993"
     CDS             complement(9470..10570)
                     /locus_tag="R8G18_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEE9796627.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(10958..11704)
                     /gene="cutC"
                     /locus_tag="R8G18_002994"
     CDS             complement(10958..11704)
                     /gene="cutC"
                     /locus_tag="R8G18_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEE9796628.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(11718..12284)
                     /locus_tag="R8G18_002995"
     CDS             complement(11718..12284)
                     /locus_tag="R8G18_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEE9796629.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            12500..14236
                     /gene="argS"
                     /locus_tag="R8G18_002996"
     CDS             12500..14236
                     /gene="argS"
                     /locus_tag="R8G18_002996"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEE9796630.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            14413..14901
                     /locus_tag="R8G18_002997"
     CDS             14413..14901
                     /locus_tag="R8G18_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEE9796631.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(15021..15413)
                     /gene="flhe"
                     /locus_tag="R8G18_002998"
     CDS             complement(15021..15413)
                     /gene="flhe"
                     /locus_tag="R8G18_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HEE9796632.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(15413..17491)
                     /gene="flhA"
                     /locus_tag="R8G18_002999"
     CDS             complement(15413..17491)
                     /gene="flhA"
                     /locus_tag="R8G18_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEE9796633.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(17484..>18417)
                     /gene="flhB"
                     /locus_tag="R8G18_003000"
     CDS             complement(17484..>18417)
                     /gene="flhB"
                     /locus_tag="R8G18_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HEE9796634.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         4639 a         4650 c         4184 g         4944 t
ORIGIN      
        1 ccgccagttg atgaaccttg gcactcaaca cctgaatcat tgagcaacct gccactgcgg
       61 actgtgcttt gagtagttcc agttggcccg ttgtgattct gatggcctta gaaccctctg
      121 caacgccctt gatagcgtca aacggtgtaa gggctgtgtg ttccttcaga atctcaccaa
      181 caagtgctgt gtggctcatg gctgggtatt tgtctttgat tgctgccagt ctggaatatg
      241 cctctggcaa aaggcgtgta actgtgtgtc tcattaaatt ttccttctta aaatctttga
      301 cttataacta actttaacat atttataaca tattgtcaac aactcattta catgttaata
      361 tggttgtagg gggctgatac aggtcattcc ctttgataaa taaggggaat taatgggctt
      421 tcttgaaaca attgctgaat ctcacagatt cgcaagctta ggttgctttg tgtttctggt
      481 gcttcttgtg tatcagcttt taaaaatgat tatcgccaga agtacacgat tcaatttcag
      541 cattaagctt ttcaaatatg aaatcacaat tacgattgat gatggtaaaa caaactaacg
      601 ttttgtgata atattggtgg cctttaagtt gctggggtca ccgtctttta atcgtagctg
      661 gtggattgaa ttccagatat caaaatgata cagcactgca aacctcaaag cgtcaatctt
      721 taaatctgtc acaccatcgt aaaagaaata acccctccct tctttctttg gtctaatccg
      781 gtaaacttcc ccgtgagtat ccttcataag atgaccttca cgagaaatgc tgtaacgctt
      841 taacaggtaa tctttgtatc tcttttcacc gtgatattct ttcattactt aattcctcca
      901 ccggcaaaat acatcttcgg tttaattttt tgcttcttgt catcctgagt ttgtttcttt
      961 ttcggtgaaa gataataatc atcttcactg tcatcgaatg ggtctaaacc cgatactttg
     1021 ttcttttgtc ttgcaagctc gaatctcttt ttcttctctg ccctcttaat ggctttttca
     1081 cgttcatcct cttcatcatc gaaggggtcc aactcatcat cagtggcggg gcactgtgtg
     1141 tttttatggt cactatcaac actcacatca gtgatttctc tgtcttgttt agaagtcttt
     1201 ttagagctta cttcaaggtc agattcattc atagagagaa aatcatttaa ttcatccaaa
     1261 gaaagaaagt catcttcttt aatatcatca ttaagtccag tctctttatt cttatctgta
     1321 atattattat ctaatgtttt atctaataac ttatctctct aacacgggtg acattttgtc
     1381 actaccccca tgacattttg tcactggtag tggtgtcatt ttgtcttact gaggctgcaa
     1441 cttcctgtga tgtaggtctt acaagtaatt ccggcttatc atccagtgtg aatacacgat
     1501 aagtgctttg acgcccctga ttcttttgca cctgaattaa accgtgtttc tcaagtgtct
     1561 caatggctct gattgctgtt ctctttgata caccagccat ataagcgaga tggtcaatac
     1621 ttggtgaata tgatttgtat ttctcttcat agaagtcaca gctatggacc atgaccatat
     1681 aaatcaatct tgctgctgcg ttaatttctt ctgaaagttg agagattcgc acatcaaatt
     1741 tataaaactg atttgggtta tatttagaca ttcataaatt acctcatacg tgaaaaggcc
     1801 aaccggataa aaccgattag cctgtgtgat taaaatatct gggggattga tggactgata
     1861 aagaagtcca tgcctgaaac agtacgatga ttaaaacgct gaggtatacc ctgaatgaga
     1921 ggttaatata cgccaaccat ctgacacctc cccaagtgtt accacttcaa gacatgaact
     1981 tttttatcag cccatataga aactataaca gatgtgatta taaattgcaa caattatttc
     2041 acagaatgat tactgattgg tcatttattg ttcacaggga tacttcttaa catctgaatc
     2101 acctgtcttg taaatgacca tatttatgag aattttagct caacctttga gcaatcctgt
     2161 atgacgtgct cagaggcatt ctgagagcat ctgcaatgta cttaatgggt tttccttgcc
     2221 tgtatagttt tatgaagctg tcgagcgtct caggtgggta aatggtgggt cttcctctta
     2281 gtccaccacg gttaatgatg gttaacggta gcaatgtatc agcacagcaa accatctgtg
     2341 taatttcatg cttacgtggt tcttttttaa aaacaacgac tgcacttgga aacggtgacc
     2401 taatagtaag cctgttatgc cctgctggtg gaaggtattt aactgcacga tgaagaatac
     2461 ggatttcatg acagtggtta ttgctgtaaa tgtgcctaag ccagtagctg gtgttggctc
     2521 tatgcggtat caggaaagct actaaatccg cttcagacgc ttttaagagg aacgacggaa
     2581 tatcgctgta cggcgggtta cagaagacct tttcactaac ccatgattga cggcttatat
     2641 catcggtgaa tcgtggtaac agcgtgtcat gctcagttgc agcaccatct agactgaaaa
     2701 taaattcacg gtgtaagttc ttaaataaca aaaatggagt tctccagtaa tcagaacccc
     2761 catgatgctt cccgattaaa atatgctcaa ttgacataag aaataaaaat ttttattgca
     2821 atcactcttg acaaggttta ctctctttta agagatactt aacaggtttt ctaaacggat
     2881 atcctttctg ggatatctat cttttatctt aaaagttttt acagatactt taaagatatc
     2941 tgtatatatt ctgtacagta acttttcaga gtctgaaaat ttttataaaa aatgactagc
     3001 cctctttgaa tctcatcttc cccgaatctt cgaaaatatc tgtctagttg attcggtaga
     3061 atctacctca tgatgtatac gtgtttagta cccgtcacac cagctatgtg acccccttgg
     3121 caaaaaagcc aaatatcagc tacatccatg taactattca gtaacttatt tagcaagtct
     3181 gtaataagtt gctctagaga ttcctagctc ttcctgaatc tgcaacctcg ttttaccttg
     3241 tgaaataagc atctgtacag cctctgaaga agcttttaca ggtcttccca tatgtacccc
     3301 ttcagcttta gcacgttcaa taccagcctt tctacgctct gctatcaagc ttctttcaag
     3361 ctgtgccaac gttgacataa tgcctaacat tgcttcaccc atagcgcttg ataagtcaaa
     3421 tccttctcta aggctgataa ctgtcacacc tttagcctgt agcgctttaa ccgttgataa
     3481 cacatcaatt gtgtcacgtc caagacggtc tacagctacc acaactacgg tgtcaccttc
     3541 tcgtgcataa gctaacaaac tgctgaaacc gtttcttaaa gaagctttga cagaaccact
     3601 gacagcttca tcagcgaacc atttagcaat gttgtaccct gcctcttcaa tttgcatttg
     3661 ctggttagtg atggattggt catctgtact caccctgcaa tatccaagta tcgccataat
     3721 gttcccctta gaagtctcaa aagttagtct cataagttta gctgtctcat aatccaaaaa
     3781 caaccttttg agactacttg agggtgcatt ctaccgttac gttgaaagtg tctcaaaagg
     3841 acttccttat gagactggtt aatagagcgc tatgaagatt caggagggta actataacca
     3901 tcactttcta actttcacgt aggaaatcgt taagtttctt gatagttaca ccatgtgacc
     3961 aactatctat atatcgttat atagtcctgt atatagcact atatgtagtg tctggagagg
     4021 gacgactaac acgatacagt ttaccctgaa aagagacttc acagattctt tatatatttc
     4081 aataagttat gctaaaaaga gactataccg atactgcata gaatttgtgt aggattgcat
     4141 agatacttgg aaaaacaccg ttaaattgca tagtcattca cctgatattg tcctgttttt
     4201 acaggtagtt gattcggatg ttctaggaat catcctcata gttgattcgg ttagttgggc
     4261 ttgtaccaac ccctaatcaa cctcatagtg tatttagacc ttgatgattt tagacagccg
     4321 ccctgttcat gcagcacgtc cacacagtga agctattcgc gacgctcaac gaaaaaaacc
     4381 taaagttccg gtacatgctg tactgacggc gacaaatccg ctgattcgtt ttatcggtag
     4441 tgatgatatg acgcaaaacc gggaattatt tcaggtctgg ttacagaaat tagcgcagtg
     4501 gcatcagacc actacgcctt atcttttttt acatacgcca gacattgccc aggccccgga
     4561 actggtacat accctgtggg aagacttacg taaaacgctt ccagagatcg gagcagttcc
     4621 ggctattcca cagcaatctt ctcttttctg aatttgccac ctatcataga caggtgccat
     4681 cggccatttt aaagggagtt tgtatggtaa gcgcgctata tgccgtttta agtgcgttgt
     4741 tattaatgaa gttctctttt gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg
     4801 gtgacggtgg ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat
     4861 atattcccat cgcgattgtg ctgatgctgt ttatggaaat gaatggcgca gaaacctgga
     4921 tggtgcatat ttgcggcatc gttttgcttg ctggtcgtct gatgcattat tacggttttc
     4981 atcaccgtct gtttcgctgg cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc
     5041 tgatggtgct ggcgaatctt tggtatatgc cctgggagtt ggttttctcc ctgcgttagc
     5101 gcacaatacg ccactttctt tttcccggat ttttacgtta tgtctcaccg cgacacgcta
     5161 ttttctgccc ctatcgccag actgggcgac tggacctttg atgaacgggt agctgaagtc
     5221 ttcccggata tgatccagcg ttccgttccc ggctattcca atattatttc catgattggt
     5281 atgttagccg agcgcttcgt tcaacctggt acgcaggttt acgatctggg ttgttctctg
     5341 ggcgcggcga cgctctcggt gcgtcgcaac attcatcatg ataattgcaa aattattgcc
     5401 atcgacaact ccccggcgat gattgaacgc tgcagtcgtc atattgacgc ctataaagcc
     5461 cctacgccag tagacgtcat tgaaggtgat attcgcgata tcgccattga aaacgcatcg
     5521 atggtggtgc tgaattttac cctgcaattc ctggaacctt ccgagcgcca ggcattactg
     5581 gataaaattt atcaaggact gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt
     5641 ttcgaagatg ccaaagttgg tgaactgttg ttcaacatgc accacgactt taaacgtgcc
     5701 aacggttaca gcgaactgga gatcagccag aagcgcagca tgctggaaaa cgtgatgctg
     5761 accgattccg tggaaaccca taaagcacgc ctgcataaag ccggttttga gcatagcgag
     5821 ctgtggttcc agtgctttaa ctttggttca ctggtggcat taaaagcaga ggacgctgca
     5881 tgatcgactt tggtaacttt tattctctga ttgccaaaaa tcatctttca cactggctcg
     5941 aaacgctgcc cgcgcagatt gctaactggc agcgcgagca gcagcacggg ctgtttaagc
     6001 agtggtccaa cgcggtggaa tttctgcctg aaattaaacc gtatcgtctg gatttattgc
     6061 atagcgtaac cgccgaaagc gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa
     6121 cgcttatgcg gaacctgatg ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca
     6181 tcgataccga atggcgttcc gactggaaat gggatcgtgt tctgccccat ctttctgatt
     6241 taaccggacg caccattctg gatgtcggct gcggcagcgg ttatcacatg tggcgcatga
     6301 ttggcgcagg cgcgcacctc gcggtgggga tcgaccctac gcaactgttt ctgtgccagt
     6361 ttgaagcggt gcgtaaacta ctgggtaacg atcagcgcgc acatttgtta ccgttaggta
     6421 ttgaacagct tccggcactg aaagcctttg ataccgtctt ttcgatgggc gtgctttacc
     6481 atcgccgttc gccgctggag catctctggc agttgaaaga tcaactggtg aatgagggtg
     6541 aactggtgct ggaaacgctg gttattgatg gcgatgagaa cacggtgctg gtaccaggcg
     6601 atcgttacgc gcaaatgcgt aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact
     6661 ggctgaagaa gtgtggtttt gtagatatcc gcattgtgga tgtgtgcgtt accactactg
     6721 aagagcagcg acgcactgaa tggatggtta ccgaatctct gtccgatttc ctcgacccac
     6781 atgatccgag taagacagtt gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc
     6841 gcaagccgta aaggtctggt attactgccg gatgcggcgt gaatgcctta tccggcctac
     6901 aaacccttgc taattcaatc tattgcaggg actatgtagg cctgataagc atagcgcatc
     6961 aggcagtttt acgtttgcat aacctcagcg cccgtttccg ggcgctattc acttcttacg
     7021 cctgaacagc aggcttatca aatgccgtta acttcggtgc gttacccgta tatttttcga
     7081 tatacaccag cgcagagtta ccggcacagg catttgccag ctgcgagctg gggatatccg
     7141 ccgttaacac gttcgcactg ccgtttttac acaagccgtt ttccagatct ggccatgcac
     7201 cttcatgcac gcataccacg ccttttttga tcccgtcagt caccaccgcg cctgtcaaaa
     7261 tctgaccacg tttgttccag acacgcacca gatcgccatt cgcaatacca aatcgagcag
     7321 catcttcggt gtgaatagtg atcggttcac gatcggcaac cgcatatttt ttacgcagtt
     7381 ccgcatagtt aagctgactg tgtaaacggt gtgccggatg tgcggtcaga agctgcaact
     7441 gcttctcgtc ggcggtaccc ttccactcat caggcgcaag ccaggttggg tgtgccgggc
     7501 aatccttata gccaaatttt tccagcgttt tggagtaaat ctcaattttg ccacttggcg
     7561 tacccagcgc atttttcacc ggatcggcgc ggaaatcacc ataacgaacg tactgttcgt
     7621 tcttctcgct gcggcgcatt tcgatcagtt tattttgctg ccagaaggca ttaaacattg
     7681 gcatagtgac gcgttgcgca cgggcccctt tctgagcggc atcatagaaa aatttcagcc
     7741 acgccatttc atctttacct tcggtataga tctcttttcc gccaggtttg agtaattccg
     7801 caagatcggc aaacacatca aaatcgtttc gcgcttcaaa ttgcggagca acagcctgct
     7861 tcatcggcac aatatgctgg ttgctgtaat caccggtcat cgtcaagtca ttgcgctcaa
     7921 acgatgtggt gatcggtaat acgatatctg cgtgtttagc agctgcggtc cagtagcatt
     7981 cagaaacgac gatcatctcc ggtttctgcc atgctttaat cagacgatta gtatcctggt
     8041 gatgggtaaa gttaccgcca cccgcccacc agatcatttt gatattcgga tacgtttgct
     8101 ctttaccgtt atgctgatat ttgccgccag gattttccag cgcatcgacg atacgggcaa
     8161 caggaatcgc cctcattccg ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg
     8221 cagacatttc cggcagcacg ccgccaacac gtgtcgggtt gccaccattg gagtaatgat
     8281 aagagaaacc aaaaccgccg cccggtgtac caatctgccc taacatcgcc gccagcgtca
     8341 ccagcatcca gtgtttctgt tcaccatatt gctggcgctg aattccccag cctgccatca
     8401 gcattgtgcg attcgccgcc atcagttcag cgagtttgac tatctgcgct tccggtacgc
     8461 cagtaatttc cgccgcccag gccgcgctct tcggcgtatt atcgctctta cctctcagat
     8521 actcttcaaa ctgcggatac ccggtagtgt atttttcaag gaacacttta tcgtgtttgc
     8581 cttgtgtcat cagggtatgc gcaatcccta acatcagtgc cacgtcggtg cccatattcg
     8641 gcgcgatcca ggtggcatta tcgccaaaga agtcgatggt ttcggagcgg ataggatcaa
     8701 tggcaatcac tggtttgcca gattttttca gctgatggaa gtattccagc ccttgctcat
     8761 cggtactgct ccaggcaatt tttaaggtat tcagcgggtt catcccccac agcaccacaa
     8821 cctggctgtt ttccagaatc agcggccagg aggtctgctg ttcatacacc tctacagaac
     8881 cgaccacatg cggcatgatc acctgtgccg ccccggttga ataatcgccg ctatgcccgg
     8941 aataaccgcc cgccaggttc atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc
     9001 cagaagagcg ccagccgtat gatccggcaa aaatagccga tggtccgtta gctttgcgaa
     9061 tgcgatcgtg ttgctcatga atcagtttta atgcttgttc ccagctcacc tgtacatagg
     9121 tatcttcgcc acgacctttc gtcggttgca gtggattatc gagatagctt tttctcacca
     9181 tcggatgctg aatacgcgtc gtggtgtgta cctgatccgc cgccgtagac tgtaaggaat
     9241 tcggtatggt tttcgccagc gcgcctgttg aagaaacaat cttgccgtcc ttcacttcta
     9301 cgttcatcgc tccccaacgt cccgcggtga ggattttacc gtccttctct tctgcccatg
     9361 cggggagcgg tgctgccgat gtcaccacca gcgctccagc ggcaataccg ctgtgtttaa
     9421 taaattcacg tcttgttaat gtcataactt cctccctgat caacgaggat cactgtttct
     9481 cggtaatatc tttggcgttg tactggaaat accgcgttaa aatgtccagt tcgttttcgc
     9541 tcatgctggt tcgtgccccc attcctttgg caatggacgg ccacgcattg acggtgtaat
     9601 ggtcggcggc aataggggca tgacaaccag cgcaataggt atcgtcaagt ttttcagcgt
     9661 attgccatag cggtttacgg tccgctaatc cgggatcggt aagcgcaccc tgtaaagacg
     9721 cctgacgcca ttgattgccg tattcgtcag cctgccattc cccgtttacc gtgagcgcct
     9781 tgataccttc ttcacttaat gtggctagcg ccagccgttg acctgccgcc aggtagagcg
     9841 tgttttcact gccctgcatt tgataaccct gcaacagaac gatcggctgt ttgccactgg
     9901 catcaacgac ggtgagatcg gtaccaggat tcacggtagc cagctcgcct atgcgagaag
     9961 ttttgaaagg ataaatatgt gcgccattag tcactgaagt agcggcctga ctttccagct
    10021 catgcgccgc gttgtcatcc atttttattt ctggcggaaa atgggcaatg cctttatgac
    10081 aatcgataca ggtttcgccg cccttttgtg ctttgttatg cattttctgc gcagattcac
    10141 tttgcgaggc aatatccatg gcatcaaaag aatggcaact acggcacgtt gcagagtcag
    10201 ttgcttttaa ttctttccat actgtttcgg ccatttcctg gcgatgagtt tcgaacttat
    10261 cgtcactgtc tattttgccg ctaacaaatt catgataaat atctttagat gcttttaatt
    10321 tagcaaataa ataatccatc cctgactttg gaatatggca atctgcacat tccgcacgta
    10381 tccctttctg gttcgaaaag tggacagttc cctgatattc ctcaaaaggt ttactcatcg
    10441 agtggcagga aaggcaaaat gctgtatccg acgttttatg taaggctttt tgcgccagca
    10501 ataacccgcc gccaccaacc acaaccgcta tcagcaaaaa caataaccca atgcgttttt
    10561 tccctcgcat agattcctct aaataaattc cagaacaccg aaatcattgt ataattaaat
    10621 acacaacgaa ttaaccgctt gcataactcg gcacaacact gcctgaaata atcgataaag
    10681 aatatgattt attacaatgt aatcattaat tgctaaggaa taacccagtt gctatttaga
    10741 atatatattt atcagtttta gcaatttaaa tcccataatc aatgtgaata taaaaaatat
    10801 tattagcaca acgctatgtt cactgtcacc cgctcaacag atcacggtta tcgttcgttt
    10861 tttatactgc tcagggataa aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc
    10921 aagcatcata ttgggcgaca tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa
    10981 tgattccttt catttcagca accgccgccc cgtctacgat ataacgcgaa tactcgtccg
    11041 cgtgttcatc tgatgacatg gacaatcctt gattacgata acgcatcggt gaggcttgcc
    11101 acgctcccgc ggagctatgg acttccagca ctccggcatc gaggaagtgg tgcaggtttt
    11161 ctgcacggac tcctgctccg gccataatga ttggagcatc acgatgggca ataagttcca
    11221 taatttttga taaaccttgc agtgcgtctg atttttgccc tgatgtcagt acccgggcaa
    11281 tgcccaattc cgcaagatta ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc
    11341 gatgaaaagt cactgccagc ggaccggcag cagccattat tttttccatt cgtggcatat
    11401 cgacattccc gtcaacatcg agaacgcccg tcaccagtcc aggaaaacct aattcgcgga
    11461 ccgtgcgcac atcctcaaga atggcggcaa actcaccgtc gctgtagcaa aaatcaccac
    11521 cgcgtgggcg aattatcgga tgcacaggga tcgtcacccg ctggcgcacg gatttcagta
    11581 cccccaacga cggcgttaag cccccctctt ttggggctgc gcataattca actctgtctg
    11641 cgccgttttg ctgcgccgtt agtgcacatt ccatgctgta acagcaaatt tccagtaatg
    11701 ccatttttgc tccttaatta cgctgactgc tcgctggcaa cgatctcttc aatggaccac
    11761 ggatgaaact taatagttgt tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc
    11821 tcatgctcac cttttggtga actggtttta actgagatcc ccggtaggct gagtccttca
    11881 tcagaaagca atgccagcgc acgggcattc agcgtttctg gatcacccag cagaacgatt
    11941 tcaatatgtt cccaaccttc gtgtgggtaa cgtttttccc cgggccacgg tagctccaca
    12001 atagaaaact gccagtgcgc aacctgtacc ggttcatgca atttaaacag acaaatcggt
    12061 ctgccattga tcatattttc tgacaaaagc tcgccacact gttcaaaccc gcgacgccag
    12121 cgttcagcag tggcgttttg atggcaacgc aaagaaatgt gatcggcagt cagcggagtg
    12181 atattcaacc ccagacggag ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc
    12241 gatgcaatat cctgcagctc gtcgatagat tgccagttcg ccataatcac tcttcgtctt
    12301 tcagtaaaag cgttaattta ccctgttgcc ctgcgccaac caaccgctga tttcacgccg
    12361 cttctgatgc aatagtgaaa acggcaatac gccacgcgca cgttgctgac gaaaacagcc
    12421 atttgcagta tactcccgcc ctaatttctt taactggtgc gggcaatttt tgctcgcttc
    12481 atcaatgtaa ggtattccgg tgaatattca ggctcttctc tcagaaaaag tccgtcaggc
    12541 catgattgcg gcaggcgcgc ctgcggattg cgaaccgcag gttcgtcatt cagcaaaagt
    12601 tcagttcggc gactatcagg ctaacggcat gatggcagtt gctaaaaaac tgggtatgac
    12661 accgcgacaa ttagcagagc aggtgctgac tcatctggat cttaacggta tcgccagcaa
    12721 agttgagatc gccggtccag gctttatcaa cattttcctt gatccggcat tcctggctga
    12781 acatgttcag caggcgctgg cgtccgatcg tctcggtgtt tctacgccag aaaaacagac
    12841 cattgtggtt gactactctg cgccaaacgt ggcgaaagag atgcatgtcg gtcacctgcg
    12901 ctctaccatt attggtgacg cagcagtgcg tactctggag ttcctcggtc acaaagtgat
    12961 tcgcgcaaac cacgtcggcg actggggcac tcagttcggt atgctgattg catggctgga
    13021 aaagcagcag caggaaaacg ccggtgatga aatggagctg gctgaccttg aaggtttcta
    13081 ccgcgatgcg aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta
    13141 cgtggtaaaa ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga
    13201 catcaccatg acgcagaacc agatcaccta cgatcgtctc aacgtgacgc tgacccgtga
    13261 tgacgtgatg ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa
    13321 agccaaaggt ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa
    13381 aaacaaggaa ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta
    13441 caccaccact gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt
    13501 gctgtattac atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg
    13561 taaagcaggc tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct
    13621 gggtaaagac ggcaaaccgt tcaaaacccg cgcgggtggt acagtgaaac tggccgatct
    13681 gctggatgaa gccctggaac gtgcacgccg tctggtggca gaaaagaacc cggatatgcc
    13741 agctgacgag ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga
    13801 tctctccaaa aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga
    13861 gggtaatacc gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa
    13921 ggcagaaatt aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga
    13981 agcgcaactg gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga
    14041 aggcacgccg catgtgatgt gtgcttacct gtacgatctg gctggtctgt tctctggctt
    14101 ctacgagcac tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa
    14161 actggcacaa ctgacagcga agacgctaaa gctgggtctg gatacgctgg gtattgagac
    14221 agtagagcgt atgtaatcga tttttcgtga gagtaaagcc tgatcagggt tacctgatca
    14281 ggctttttta ttgccatcta aatgtattct gaaaatggac atgccattgt tttctgactg
    14341 ttggataaga ggccagaagc gtaatatccg gccccaggga aacgataacg gttgaattta
    14401 aggaataccg cagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct
    14461 tacgcagaag atacggttat agaaaacgac catgacattt cagccatcaa agattgttgg
    14521 caaaaaaatt cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa
    14581 tacaatctcg tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa
    14641 caagtgccac gcacaggtgt aaaaaaacct gataccgaac aacttaactt gcttaaaaaa
    14701 tcacagcgag cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag
    14761 gacgttcagg atttaagtga cccttattct gaatcagaat ggctctcatg tatgatcata
    14821 cagaccaata cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca
    14881 agccctttga caagaggata attcacatct ttttggcatg ttttgttgca agctattcct
    14941 gataaataat tgcaacaaga catcgagcct ttttcactga gttattaaac atactcgcga
    15001 gcgcgtaatt tttttgtcct tcagcgataa ttcacaatca cttcattacg ttgtaccttt
    15061 agcggtggaa ttaaccgccc accgcctggc acttcccaga taaatcgcaa cggttctgct
    15121 gccgctatac cggaaaaggc cacggtggtt ccgctctgcc cctctaattc gacacaacga
    15181 gattgcgaac acaagcgaac ccgcagtcct gaaggtgtcg ggccgataag ctgataacgc
    15241 cacgctacca gcgtcatcaa tcctgaagca ggttgtcgcg tagaaagagg cgcagacgac
    15301 atcgactcac cgcgatgatt tagcgtaatt cctacactac ttgcctgcca catcccctcc
    15361 ccggcggctt gcaccagcag cggaaacaat aatattgcta ataaggttct cattatttgc
    15421 cgccaattgt cgccgtcatg cggatatgtc ggttatcaga cagttccaga ttcgacagga
    15481 ccactaactg cggcaagctg cggcgcagga agcgagacaa taatggtcgc agcgcgtggt
    15541 tcaccaacaa tactggcggc gcgcccagca tctcctgacg ggatagcgct tcctgagttt
    15601 gcgccagtaa acgatccgcc agccctggct ccagtcctcc cccgccctgc agcgcctgta
    15661 gtaacaaacg ttccagcggt gtatcgaggc caataacatg gacttcatct ttgccaggaa
    15721 accactgctg ggtaatcgcc cgtcccaacg ccacgcgcac gacggcggtt aattcatgtg
    15781 gatcgctttg gatgggcgca tgttccgcca gcgtttcgag aatggtgcgc atatcgcgaa
    15841 tcggtacttt ttcatcgagg agattttgca gcactttatg cagtgtggtg agcgtgacga
    15901 cgccaggaac gagatcttcc gtcagctttg gcatctcctg ggcgacgcga tccaacagct
    15961 gttgcgcctc ctgacgacca aacagctctg cggcatgctg gctaatgagg tggttaagat
    16021 gcgttgctac caccgtgctg gcctcaacca ctgtgtaccc ctgaatctgc gcctgttctt
    16081 ttagcgcact ttcaatccag atagcattca ggccaaatgc cggatcgacg gtcgcctcac
    16141 caggtaacgt cccggcagcg gttccagggt taatcgccag ccagcgcccc ggataagcat
    16201 caccactgcc aatctccacg cctttcatca aaatgcgata gcgggcaggt tgcagatcca
    16261 tattgtcgcg aatgtgcacc actggcggca gaaatcccat ctcctgggca aatttcttgc
    16321 ggatactgcg tatacggccc aacaactcac catcctgctg gaaatcgacc atcgggatca
    16381 gtcgataacc cacttccatt cccagagaat cttccagttg tacatcgttc cacgtcgctt
    16441 cgacaacggt attattctct gccattttta ccggtttggg ttcggcaggc gctttttgtt
    16501 cgcgtccgcg tatccaccag gccagcccga gcaatccggc agtgaacagc aaaaatacca
    16561 ggttcggcat tccaggcacc aggccgagta aaccgagcac ggcggcgctt aacaacataa
    16621 cgcttgggtt actgaaaagc tgattcacca tctgctcgcc aacatcctga tcggtgctga
    16681 cacgcgtaac gatgaccccc gcggcggtag aaatcaccag cgccggaatt tgtgccacca
    16741 gaccgtcgcc aatggtcaat agcgtataac tttccgccgc gtgtcccatg ctcatgccat
    16801 gttgcagcac gccgaccagc aacccgccga caacgttaat gaccatgatg aggatcccgg
    16861 cgatggcatc gccgcgaaca aacttacttg ccccgtccat tgagccgtaa aaatcggctt
    16921 cctgagtcac ttcggagcgg cgttttttcg cctcatcttc accaatcaat ccagcgttaa
    16981 ggtcggcgtc aatcgccatc tgcttacccg gcataccatc gagaacaaag cgcgcaccca
    17041 cttctgcgat acgcccggca cctttggtaa tgaccataaa gttgatgatc acgagaatga
    17101 caaacaccac gataccgata gcgaaattgc caccaacgag gaagtgaccg aacgcttcga
    17161 ccaccttccc tgccgccgcc gcgccggtat gcccttccat caaaatgata cgggttgaag
    17221 ccacgttaag tgccagacgc aacaacgtag taaacaacag aatggtcgga aacgcagcaa
    17281 actcaagcgt gcgctgggta aacatcgcca ccagcaacac catgatcgac aaggcaatat
    17341 tgaaggtaaa caacaggtcg agtatgaatg cgggcagtgg cagcaccatc atcgacaaga
    17401 tcaacaggat caaaatcggt ccggcaagga tctgccattg tgtcgatttc aggtttgcgg
    17461 gcaggcgcag catcgcggcc agattactca tgggtcggtt tctcgttaat aaaatccagg
    17521 gcttccggca ccggaagatg agtaggttgt acagggcgct gtccaccagc cagacgccag
    17581 cgtttcagtt gccagaccca ggccagcact tccgccaccg cggcgtacag ttgacccggg
    17641 atttgttgac caatctccgc atgtcgatac agcgctcgcg ccagcggcgg cgcttcaagc
    17701 gtcgggacgt tattttcagc gccaatttca cgaatgcgca gcgcgaccag ccctgcacct
    17761 ttagcgacca ctttcggtgc gctcattttg ttttcgtcat actgcaacgc taccgaatag
    17821 tgggtcgggt tattgacaat gacatcagct ttcggcacat cggccatcat ccgacgccgt
    17881 gcagcagctc gctgcatctg acggatccgc cctttaacat gagggtcacc ttcgctttgt
    17941 ttgaactcat cacgaatatc ctgccgcgac atacgcagct ttttcaggtg gctgaagatt
    18001 tggaaaaaga cgtcaaatcc caccattgga atgacaccaa gcaccaccag cagtgcgcat
    18061 agcccaacca gatccatcgc attacccatg gcggtagtcg gagactcggc cattaagcgc
    18121 atcatctgcg gccagtgatg ccagagataa aaccccgtca cgctgccaac caggatggtt
    18181 ttcaaaattg ccttaagcaa ctccgcgcca gtctgtgccg agaacatccg tttaatgccc
    18241 ggtagcgggt tgagtttgga aaacttcggc tgcaaggatt tgccgctaaa taccagcccc
    18301 cccagcatga ccggagaaat gagcgccacc agcaccacgc cgctaatcag cggcagcagc
    18361 gccagcatgg cttctctgat cagcagaata atctgcccga ggatcagatt cgggtca
//
LOCUS       DAQSWQ010000084        18211 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.084, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000084 DAQSWQ010000000
VERSION     DAQSWQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.084"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            15..1091
                     /locus_tag="R8G18_003001"
     CDS             15..1091
                     /locus_tag="R8G18_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HEE9796635.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="R8G18_003002"
     CDS             complement(1484..1825)
                     /locus_tag="R8G18_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HEE9796636.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="R8G18_003003"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="R8G18_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HEE9796637.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="R8G18_003004"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="R8G18_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HEE9796638.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="R8G18_003005"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="R8G18_003005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HEE9796639.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /locus_tag="R8G18_003006"
     CDS             3559..4215
                     /locus_tag="R8G18_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEE9796640.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="R8G18_003007"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="R8G18_003007"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HEE9796641.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="R8G18_003008"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="R8G18_003008"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HEE9796642.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="R8G18_003009"
     CDS             complement(7167..7826)
                     /locus_tag="R8G18_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEE9796643.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /locus_tag="R8G18_003010"
     CDS             complement(8153..8509)
                     /locus_tag="R8G18_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HEE9796644.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="R8G18_003011"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="R8G18_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HEE9796645.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="R8G18_003012"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="R8G18_003012"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEE9796646.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="R8G18_003013"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="R8G18_003013"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEE9796647.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="R8G18_003014"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="R8G18_003014"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEE9796648.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="R8G18_003015"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="R8G18_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEE9796649.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="R8G18_003016"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="R8G18_003016"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEE9796650.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="R8G18_003017"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="R8G18_003017"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEE9796651.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="R8G18_003018"
     CDS             complement(17270..18112)
                     /locus_tag="R8G18_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEE9796652.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4668 a         4574 c         4393 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat a
//
LOCUS       DAQSWQ010000085        17956 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.085, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000085 DAQSWQ010000000
VERSION     DAQSWQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.085"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(227..1201)
                     /gene="cysB"
                     /locus_tag="R8G18_003019"
     CDS             complement(227..1201)
                     /gene="cysB"
                     /locus_tag="R8G18_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HEE9796653.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1411..4008)
                     /gene="topA"
                     /locus_tag="R8G18_003020"
     CDS             complement(1411..4008)
                     /gene="topA"
                     /locus_tag="R8G18_003020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEE9796654.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4388..4639
                     /locus_tag="R8G18_003021"
     CDS             4388..4639
                     /locus_tag="R8G18_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HEE9796655.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="R8G18_003022"
     CDS             complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="R8G18_003022"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEE9796656.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5944..6702
                     /locus_tag="R8G18_003023"
     CDS             5944..6702
                     /locus_tag="R8G18_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HEE9796657.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6699..7289
                     /gene="cobO"
                     /locus_tag="R8G18_003024"
     CDS             6699..7289
                     /gene="cobO"
                     /locus_tag="R8G18_003024"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEE9796658.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="R8G18_003025"
     CDS             complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="R8G18_003025"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HEE9796659.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8414..9528)
                     /locus_tag="R8G18_003026"
                     /pseudo
     CDS             complement(8414..9528)
                     /locus_tag="R8G18_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9556..10176)
                     /locus_tag="R8G18_003027"
     CDS             complement(9556..10176)
                     /locus_tag="R8G18_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEE9796660.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10173..11054)
                     /gene="yciV"
                     /locus_tag="R8G18_003028"
     CDS             complement(10173..11054)
                     /gene="yciV"
                     /locus_tag="R8G18_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HEE9796661.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11192..11236
                     /gene="trpL"
                     /locus_tag="R8G18_003029"
     CDS             11192..11236
                     /gene="trpL"
                     /locus_tag="R8G18_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HEE9796662.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11213..11306
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11328..12890
                     /gene="trpE"
                     /locus_tag="R8G18_003030"
     CDS             11328..12890
                     /gene="trpE"
                     /locus_tag="R8G18_003030"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEE9796663.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12890..14485
                     /gene="trpD"
                     /locus_tag="R8G18_003031"
     CDS             12890..14485
                     /gene="trpD"
                     /locus_tag="R8G18_003031"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HEE9796664.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14486..15847
                     /gene="trpCF"
                     /locus_tag="R8G18_003032"
     CDS             14486..15847
                     /gene="trpCF"
                     /locus_tag="R8G18_003032"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HEE9796665.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15859..17052
                     /gene="trpB"
                     /locus_tag="R8G18_003033"
     CDS             15859..17052
                     /gene="trpB"
                     /locus_tag="R8G18_003033"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEE9796666.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17052..17858
                     /gene="trpA"
                     /locus_tag="R8G18_003034"
     CDS             17052..17858
                     /gene="trpA"
                     /locus_tag="R8G18_003034"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEE9796667.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4317 a         4749 c         4488 g         4402 t
ORIGIN      
        1 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
       61 tgaaaactgg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
      121 aaatatgagg gataaaaata ttgtgcacgg tatagaaata agtaacaaaa gagaatatat
      181 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
      241 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      301 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      361 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      421 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      481 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      541 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      601 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      661 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      721 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa tcagcattgc
      781 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      841 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      901 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      961 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
     1021 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
     1081 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
     1141 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1201 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1261 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1321 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1381 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1441 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1501 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1561 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1621 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1681 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1741 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1801 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1861 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1921 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1981 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     2041 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     2101 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     2161 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2221 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2281 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2341 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2401 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2461 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2521 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2581 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2641 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2701 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2761 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2821 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2881 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2941 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     3001 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     3061 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     3121 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3181 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3241 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3301 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3361 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3421 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3481 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3541 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3601 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3661 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3721 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3781 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3841 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3901 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3961 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     4021 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     4081 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     4141 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4201 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4261 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4321 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4381 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4441 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4501 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4561 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4621 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4681 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4741 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4801 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4861 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4921 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4981 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     5041 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     5101 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     5161 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5221 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5281 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5341 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5401 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5461 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5521 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5581 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5641 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5701 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5761 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5821 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5881 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5941 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     6001 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     6061 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     6121 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6181 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6241 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6301 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6361 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg tcagggacga
     6421 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6481 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6541 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6601 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6661 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6721 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6781 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6841 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6901 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6961 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     7021 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     7081 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     7141 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7201 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7261 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7321 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7381 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7441 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7501 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7561 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7621 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7681 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7741 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7801 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7861 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7921 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7981 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     8041 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     8101 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     8161 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8221 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8281 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8341 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8401 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8461 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8521 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8581 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8641 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8701 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8761 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8821 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8881 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8941 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     9001 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     9061 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     9121 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9181 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9241 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccatcag gctgcatcca
     9301 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9361 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9421 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9481 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9541 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9601 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9661 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9721 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9781 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9841 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9901 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9961 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
    10021 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
    10081 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
    10141 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10201 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10261 agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10321 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10381 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10441 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10501 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10561 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10621 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10681 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10741 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10801 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10861 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10921 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10981 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    11041 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    11101 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    11161 tacgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11221 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11281 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11341 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11401 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11461 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11521 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11581 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11641 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11701 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11761 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11821 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11881 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11941 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    12001 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    12061 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    12121 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12181 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12241 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12301 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12361 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12421 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12481 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12541 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12601 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12661 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12721 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12781 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12841 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12901 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12961 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    13021 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    13081 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    13141 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13201 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13261 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13321 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13381 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13441 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13501 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13561 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13621 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13681 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13741 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gcggcctgtg ggctgaaagt
    13801 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13861 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13921 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13981 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    14041 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    14101 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    14161 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14221 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14281 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14341 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14401 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14461 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14521 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14581 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14641 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14701 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14761 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14821 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14881 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14941 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    15001 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    15061 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    15121 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15181 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15241 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15301 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15361 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15421 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15481 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15541 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15601 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15661 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15721 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15781 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15841 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15901 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15961 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    16021 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    16081 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    16141 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16201 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16261 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16321 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16381 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16441 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16501 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16561 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16621 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16681 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16741 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16801 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16861 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16921 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16981 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    17041 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    17101 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    17161 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17221 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17281 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17341 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17401 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17461 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17521 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17581 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17641 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17701 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17761 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17821 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc ccccagccgc cagttccgct
    17881 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17941 ctttttaccg tttcgc
//
LOCUS       DAQSWQ010000086        17448 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.086, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000086 DAQSWQ010000000
VERSION     DAQSWQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.086"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..502)
                     /locus_tag="R8G18_003035"
     CDS             complement(<1..502)
                     /locus_tag="R8G18_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEE9796668.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="R8G18_003036"
     tRNA            complement(688..772)
                     /locus_tag="R8G18_003036"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="R8G18_003037"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="R8G18_003037"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HEE9796669.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="R8G18_003038"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="R8G18_003038"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEE9796670.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="R8G18_003039"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="R8G18_003039"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HEE9796671.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="R8G18_003040"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="R8G18_003040"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEE9796672.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="R8G18_003041"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="R8G18_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HEE9796673.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFKPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="R8G18_003042"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="R8G18_003042"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HEE9796674.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="R8G18_003043"
     CDS             7117..8619
                     /locus_tag="R8G18_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEE9796675.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="R8G18_003044"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="R8G18_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEE9796676.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="R8G18_003045"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="R8G18_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEE9796677.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="R8G18_003046"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="R8G18_003046"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEE9796678.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="R8G18_003047"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="R8G18_003047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEE9796679.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="R8G18_003048"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="R8G18_003048"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEE9796680.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="R8G18_003049"
     CDS             complement(16186..17382)
                     /locus_tag="R8G18_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HEE9796681.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4336 c         4455 g         4174 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccacttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taagcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac acgctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAQSWQ010000087        17338 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.087, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000087 DAQSWQ010000000
VERSION     DAQSWQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.087"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            92..1132
                     /locus_tag="R8G18_003050"
     CDS             92..1132
                     /locus_tag="R8G18_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HEE9796682.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="R8G18_003051"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="R8G18_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEE9796683.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="R8G18_003052"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="R8G18_003052"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HEE9796684.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="R8G18_003053"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="R8G18_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HEE9796685.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="R8G18_003054"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="R8G18_003054"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEE9796686.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="R8G18_003055"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="R8G18_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HEE9796687.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="R8G18_003056"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="R8G18_003056"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEE9796688.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="R8G18_003057"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="R8G18_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HEE9796689.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="R8G18_003058"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="R8G18_003058"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEE9796690.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="R8G18_003059"
     CDS             complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="R8G18_003059"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEE9796691.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12944..14125)
                     /locus_tag="R8G18_003060"
     CDS             complement(12944..14125)
                     /locus_tag="R8G18_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEE9796692.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14380..15609
                     /locus_tag="R8G18_003061"
     CDS             14380..15609
                     /locus_tag="R8G18_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HEE9796693.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16069..16701
                     /locus_tag="R8G18_003062"
     CDS             16069..16701
                     /locus_tag="R8G18_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9796694.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLHLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            17035..>17338
                     /locus_tag="R8G18_003063"
     CDS             17035..>17338
                     /locus_tag="R8G18_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEE9796695.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4596 a         4522 c         3988 g         4232 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccact atgccgacaa
    11821 ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt tttcaccaac
    11881 catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctggg
    11941 ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat
    12001 catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg
    12061 ctcgtgagtg tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc
    12121 catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg
    12181 gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt
    12241 ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg gagtggcggt
    12301 atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg
    12361 accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa tattgataac
    12421 acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc
    12481 aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct
    12541 gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag
    12601 cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta cgtccacatc
    12661 aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt
    12721 tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt
    12781 ttcattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa
    12841 tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag
    12901 attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa
    12961 tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat
    13021 tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg
    13081 ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca
    13141 tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat
    13201 cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc
    13261 ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta
    13321 aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta
    13381 tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg
    13441 ctaaaccttc tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct
    13501 gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg
    13561 ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc
    13621 catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc
    13681 acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt gcaccggtgc
    13741 ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa
    13801 tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta agtccggaac
    13861 cgcaaacgtc attaatcgtg attgcagaaa cgctattagg aagaccacct ttaatagccg
    13921 attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat
    13981 ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa
    14041 gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt gtccgtaacg
    14101 ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa
    14161 tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa
    14221 tatgcctgag cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac
    14281 caatagatgg gtagataagg cgttaatctc ccatacttaa cctggtttat ggtaaattgc
    14341 cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa
    14401 gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg cacgctcttc
    14461 cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc
    14521 tggcctttaa catattggcc acataaagcc ttatgccagt tcatcctctc atcaaaaaca
    14581 tcagcatgtg aagggataac gggcgcggta acacactact atggcaagaa gattggtaat
    14641 ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca
    14701 attaccaact cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt
    14761 atgttggtga gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat
    14821 gccactattc gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt
    14881 tccatttacc ttgtcggtag ttggcaacct gatctattaa cgacccaggt agagttcaat
    14941 cagaataccc ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat
    15001 acgcccatta tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg
    15061 gataaatgca aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc
    15121 tttgtcttta gctgcctgct ttctattcca ccttcgtata ttgaagcggc taaagaagaa
    15181 ggggtcacca ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt
    15241 tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc
    15301 gttattgaag gtgccacgga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa
    15361 agtcgtgcat ttaaccgcgc actatcaatt atgttggtat cgctgattac cttcattgtt
    15421 tgttgcatta acccgaacgc gatttcgatg atttatgcga tcagcggccc gctcattgcc
    15481 atgatacttt tcatcatgcc tacgttgtca acgtatctga tcccggcgct taaaccctgg
    15541 cgctccatcg gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc
    15601 tttagctaat atccgttgtg gatggttcaa aattgctaca attttgaacc attccatttt
    15661 tcataatatt catttgttat tattccgccc tatcacttct ctcgtcatat cctagaatta
    15721 tcagttagtt aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat
    15781 aacttagatt cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct
    15841 cataccatga gatagttatc tggaccacgt tcgaccatgc aagattaaat cagtgaaaca
    15901 taatcatatt taataacgag aaatgcattt ttaaatgtct taatcagaat tatagcaaat
    15961 acagattaat cgcaattttt cccagcaaaa ttacaaaatc aagatgtatt gacatcatca
    16021 atcaccaatg aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt
    16081 agtcgttata aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa
    16141 gagtcaccgc gtttgcagta ttgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt
    16201 tcatatttta atcgtggtgt ggtggaaggt atcttaacaa aaaaccacaa tgcgagatta
    16261 agcggatata tttttgtgga tttttcagta agttttttac atctattcct tgaaaaagac
    16321 tggattgact atcttgcaag tactgacatg gggatagttt tagtcagcga ccgcaatatg
    16381 caatcgttag ctaactactg gcgaaaacac aacaccgcaa tatctgctgt catatataat
    16441 gatgatggtc ttgatgttgc caatgaaaag atcagacaac tatttattgg tcgttattta
    16501 tcatttacca gaggtaacac attaactcag atggaattca ccataatggg gtatatggtt
    16561 tctggttata atccatatca gattgctgaa gtgctggata tggatatccg tagcatctac
    16621 gcgtacaagc aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt atttattcgt
    16681 tcacattcgg tccaacattg atactcaacg accagccaga atcatactct gttcatacgg
    16741 gcatctattg aaatgcccgt attttataaa taagcagtaa ccccaagagt aaacaacata
    16801 gtatttaaaa ccatcacacg aaaaattcag aagcattacg aaatatgaat ttacaaacaa
    16861 tagtggcata aatgttaacc atgttaattt acgtaaagtt ttacgttgca acattaaagc
    16921 ctcatttcaa tcatcatgat aaatataaaa ttaatatata tttatgccgt aaataccaat
    16981 atacttagca aactatgtga tctccatttc gattgattta gtgtttattg acgtatgtac
    17041 tggattatta acgataatat cgagttctgg cctgagcacc gaaaattaat atcggtacat
    17101 aacgccgatc ttaacgtcgt tctgacaacg ccagccagtc gatgtttatc acttctactt
    17161 gaagcttttc ctgatgtggt tacacaacaa gattttttca ccagagtctg ggaagaagaa
    17221 ggtatgcgtg tgcctactaa cacgttatat cagaacatat ccattatcag acgcggattt
    17281 cgcgctgttg gtgatactac ccactcgcta attgcaaccg tgccgagaga ggattcaa
//
LOCUS       DAQSWQ010000088        17139 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.088, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000088 DAQSWQ010000000
VERSION     DAQSWQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.088"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="R8G18_003064"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="R8G18_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEE9796696.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="R8G18_003065"
     CDS             2218..2766
                     /locus_tag="R8G18_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HEE9796697.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="R8G18_003066"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="R8G18_003066"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HEE9796698.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="R8G18_003067"
     CDS             complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="R8G18_003067"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HEE9796699.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="R8G18_003068"
     CDS             complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="R8G18_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HEE9796700.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="R8G18_003069"
     CDS             complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="R8G18_003069"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEE9796701.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="R8G18_003070"
     CDS             complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="R8G18_003070"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEE9796702.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9764..12376
                     /gene="pepN"
                     /locus_tag="R8G18_003071"
     CDS             9764..12376
                     /gene="pepN"
                     /locus_tag="R8G18_003071"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEE9796703.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12583..13350)
                     /gene="ssuB"
                     /locus_tag="R8G18_003072"
     CDS             complement(12583..13350)
                     /gene="ssuB"
                     /locus_tag="R8G18_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HEE9796704.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13347..14138)
                     /gene="ssuC"
                     /locus_tag="R8G18_003073"
     CDS             complement(13347..14138)
                     /gene="ssuC"
                     /locus_tag="R8G18_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HEE9796705.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14150..15297)
                     /gene="ssuD"
                     /locus_tag="R8G18_003074"
                     /pseudo
     CDS             complement(14150..15297)
                     /gene="ssuD"
                     /locus_tag="R8G18_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15294..16253)
                     /gene="ssuA"
                     /locus_tag="R8G18_003075"
     CDS             complement(15294..16253)
                     /gene="ssuA"
                     /locus_tag="R8G18_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HEE9796706.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16246..16821)
                     /gene="ssuE"
                     /locus_tag="R8G18_003076"
     CDS             complement(16246..16821)
                     /gene="ssuE"
                     /locus_tag="R8G18_003076"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEE9796707.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4295 a         4421 c         4349 g         4074 t
ORIGIN      
        1 tacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgccgtt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgcgacg tagtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccggtcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcacttgc aacgccgccg ctctcggtga ttaaccaatg gcagctggcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg cgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggggcagat
     1201 ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccggccta ttcgctggtc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcccttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta cctccaactc tggcacgcaa agatattctg
     1501 ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg caggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt tccgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tggtcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcag ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttctattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attatccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat tgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttaatctggt gtgaacaaac ccgtctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatcc acacttggtt atgaacgcct gcataatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct tttaatgcgg gcctggattt
     3481 ctggcaaagt gcgctttgtt tatgccggat gcggcacgag cgccttatcc ggcctacaaa
     3541 atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct gataagcgta gcgcatcagg
     3601 caatgttgcg tttgtcatca gtctcagccc gcttttcagc gggcttcatt gtttttaatg
     3661 cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt gtcatcccgg
     3721 ccacatttac gcgaccagaa gcaaccgcat atacgccaaa ctcttcgcgc agacgcagca
     3781 cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg ataaagctga
     3841 agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc atacgctgaa
     3901 tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca tcgttgctca
     3961 ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta gcgcgaatcg
     4021 ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca accagagtac
     4081 aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg gcaacaatca
     4141 gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc agaccacggg
     4201 caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaacg gagagttgtg
     4261 ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttatgg cagcagccat
     4321 ggaacagcac tacgtcgcca gcctgagctt cattcaggct gttaatcagt gcatcgaagt
     4381 caagagtgtg attttccgca tcataataag cgtattcacg aacttccaga cctgcagagt
     4441 taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc ttaacgctgg
     4501 tattttttgc caggaaatcg gcagctacgc gtagtgcgcc agtgcccccc ggagtctgtg
     4561 ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc agttcctgag
     4621 tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg gtttcatttt
     4681 cgagcagata ctgttcagcc tttttcacgc tggtcagtac cggggttttg cccgtctcat
     4741 ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca cgaaacagat
     4801 cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac atgacgaggt
     4861 tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt acaaaattcc
     4921 aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct ctggaaggta
     4981 taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt gtgctattag
     5041 aactggtaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag tttgttgtca
     5101 gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt gaagtagtag
     5161 gttgcgccca cttcaaagta gttcaccagc tcaacatcac cgataccttc tacgtctttc
     5221 gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg gtattgcgca
     5281 actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa tttattagtg
     5341 atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt gttcgcgtcg
     5401 tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg ttgagcttct
     5461 tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc ttcgtattcg
     5521 tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc acgctcgttt
     5581 ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa gttggagtta
     5641 cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta tgcagtatca
     5701 ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac accgtagtta
     5761 cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc cagacgcgtt
     5821 ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt atattcccac
     5881 tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc aagacgggca
     5941 taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga aaaataatgc
     6001 agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata gatttctgca
     6061 gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat attgcgcttc
     6121 atcattattt attaccctca tggttttttt tatgacacct gccactgcgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc
     6361 atatttccat agaaacgtga tgatatttac agcaattttt atctatttat atgatttact
     6421 tatatttaaa ttaactaaac ggaaattttg tttctgatgg aaactttatc gacctggcac
     6481 aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt gctatcagct
     6541 aagaaaaaac taatccgcaa taagaatgcg ggttattttt tgtgcgtaaa tgttgcaatg
     6601 taatattccg taacaggatg atcgttatgg cggggttgca aatagattgc ttgacttaac
     6661 aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct ctttgaggaa
     6721 gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc acgcacgttt
     6781 tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc tgaatgcgga
     6841 acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt caggcccatt
     6901 tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc accaatgatc
     6961 tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc ttcgttgagg
     7021 cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg tgctttaaag
     7081 tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca gtaaaccggg
     7141 ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta atccacctgc
     7201 gcgaaatcgg cctcaatgaa gcgttccaga cgtgaaacgg catctttatc tacgcgttca
     7261 gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac atatttcagc
     7321 atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc cggctccagc
     7381 atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa agtcgggccg
     7441 aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg gccagatacg
     7501 gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc ctgatcgtta
     7561 cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc accttcggta
     7621 tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt aaagaagcga
     7681 tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag gtttgtgcgc
     7741 ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc cgccatcggg
     7801 taagtgtctg gatcttcaac ccaaccagca acttcaacct tgctggcctg aatttcaaat
     7861 tgttgcccct ggcccggcga tgccacgact ttacccgtca caatgaccga gcagccggtg
     7921 gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac agcctgtaca
     7981 ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt tgaatctcgg
     8041 cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg gccctggagt
     8101 acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg aaaaaataaa
     8161 cacttgtcca cccgtaaatg ggggggatac ctatgttacc tggcatctgc aatcagacaa
     8221 gcagaattcg caaatgcagc gaaagatttc ggaattaaag gtaaaaaaag ggagccgatt
     8281 agctccctga tgatattaac tggctttttt aatatgcgga aggtcgaacg ctttgcgcag
     8341 cgcccgaaca aacgctttat catggcagat agttttgcca gggctgtcag aaagtttcgc
     8401 caccggttta ccgttacact ctaccaactt aatcacaata ttcaggggtt ttacctgggg
     8461 gatatcgcag gtcaggcgag tcccaatacc aaaacttaat tgcacgcggg aagagaagtg
     8521 gcggtatagc tcaaccgctt tgcgtaaatc cagattgtca gagaaaacca gcgttttact
     8581 ctgtggatca attcccagct tttcataatg tgcaatggct ttttcacccc attcaaccgg
     8641 gtcgccagag tcatgacgca ggccctgata ccgactagcg aactcgacac cgaaatcacg
     8701 caggaaagca tccatagtga tgcagtcggt taatgcaatg ccaagttggt cgggatactc
     8761 ttccagccag gcagcaagtg cagctcgctg gctgttggct agatccgggc tgatttgctg
     8821 atgtgcctgg aaccattcgt gtgcctgtgt tcccatcggc gtgagggaaa gccgacgcgc
     8881 cagatcgtag ttgctggtgc ccacgaacca ggattcctgt tgcagacgct taacgatggt
     8941 ttcttgtact tcgcgagaaa aacggcgacg ggtgccaaaa tccatcagat ggaagcgcga
     9001 catatcaaga ccggcggtta acgccgagaa gtcgactaat ttgctttcca gcgtgtcgag
     9061 ggcttgcgca acgtcggcct gcggtgagcg atagcgatgt accatttcac tgataaccgc
     9121 cagcaaagga acttcccaga ggatgacttc acgccacgga ccgcttaagc gaatatccag
     9181 cttgccatta tcgttggaca cggtgacttg ttccgggtta aagcggaact cgcgtaacca
     9241 gttaagatag tcggccttaa agaaaggcag ggcagagagc cactgatatt catcatcctg
     9301 caggcgcagg tgctgcatcg cctgaatctg ttcacgaata gcatcggcat aaatacccag
     9361 cagatcgtca cctcggcaac gaaactccgc cgcgacatgc acatcgtaat agtgatgaaa
     9421 cacggcttgc tgcatatgca acttataagc atctgtatcc agcaacgagt gcagaacagg
     9481 agaagcgaat tgtgtcatag gtgcgcagta gcgtcctcgt acaggagcgt ttagtacaat
     9541 aaacatcttc aggaactgct ggagtatacc ttgtttcgca atttattgaa ccccgatcac
     9601 accatatgcc acctttctgg tcgatggcat tacgcggtgt atgttataaa aatgtagcaa
     9661 taaaggcgtt tgtacctgaa aagatgaaga ttctgcatag cgcgatttac gcaacaggaa
     9721 tagactgaac accagactct ataaaagatg ctaaaggtta tttatgactc aacagccaca
     9781 agccaaatac cgtcacgatt atcgtgcgcc ggattaccag attactgata ttgacttgac
     9841 ctttgacctc gacgcgcaaa agacggtcgt taccgcggtc agccaggctg tccgtcatgg
     9901 tgcatcagat gctccacttc gtctcaacgg cgaagacctc aaactggttt ctgttcatat
     9961 taatgatgag ccgtggaccg cctggaaaga agaagagggc gcactggtca tcagtaattt
    10021 gccggagcat tttacgctta agatcgttaa tgaaataagc ccggcggcga ataccgcgct
    10081 ggaagggctt tatcagtcag gcgatgcgct ttgcacccag tgtgaagccg aaggtttccg
    10141 ccatattacg tattatctcg accgcccgga cgtgctggcg cgttttacca ccaaaattat
    10201 tgccgataaa accaaatatc ccttcctgct ttccaacggt aaccgcgttg cgcaaggtga
    10261 actggaaaac ggacgccatt gggtacagtg gcaggacccg ttcccgaaac cgtgctacct
    10321 gtttgcgctg gtggcaggcg actttgatgt actgcgcgac acctttacca cgcgttctgg
    10381 tcgcgaagtg gcgctggagc tgtacgtcga tcgcggcaac cttgatcgcg cgccgtgggc
    10441 gatgacctcg ctgaaaaact caatgaaatg ggatgaagaa cgcttcggcc tggagtatga
    10501 cctcgacatc tatatgatcg tcgcggtgga tttcttcaat atgggcgcaa tggagaataa
    10561 gggtctgaat atctttaact ccaaatatgt gctggcccgc accgacaccg ccaccgacaa
    10621 agattacctc gatattgaac gcgttatcgg ccatgaatat ttccataact ggaccggtaa
    10681 ccgagtgacc tgccgcgact ggttccagct cagcctgaaa gaaggtttaa ccgtcttccg
    10741 cgatcaggaa ttcagctctg accttggttc ccgcgcagtt aaccgcatca ataatgtgcg
    10801 caccatgcgc ggattgcagt ttgcggaaga cgccagcccg atggcgcacc caatccgccc
    10861 ggacatggtc attgagatga acaacttcta caccctgacc gtttacgaga agggcgcgga
    10921 agtgatccgt atgatccaca ccctgctggg cgaagaaaac ttccagaaag ggatgcaact
    10981 ctatttcgag cgtcatgatg gtagcgcagc gacctgtgac gactttgtgc aggcgatgga
    11041 agatgcgtcg aatgttgatc tctcccattt ccgccgttgg tacagccagt ccggtacgcc
    11101 gattgtgacc gtcaaagacg actacaatcc ggaaactgag cagtacaccc tgaccatcag
    11161 ccagcgcacg ccagccacgc cggatcaggc agaaaaacag ccgctgcata ttccgtttgc
    11221 catcgaattg tatgacaacg aaggcaaagt gatcccgttg cagaaaggcg gtcatccggt
    11281 gaattccgtg ctgaacgtca cccaggcgga acagaccttt gtttttgata atgtctactt
    11341 ccagccggtg cctgcgctgc tgtgcgaatt ctctgcgcca gtgaaactgg aatataagtg
    11401 gagtgatcag caactgacct tcctgatgcg tcatgcgcgt aatgatttct cccgttggga
    11461 tgcggcgcaa agtttgctgg caacctacat caagctgaac gtcgcccgtc atcagcaagg
    11521 gcagccgctg tctctgccgg tacatgtggc tgacgctttc cgcgcggtgc tgctcgatga
    11581 gaagattgat ccggcgctgg cggcagaaat cctgacgctg ccttctgtca atgaaatggc
    11641 tgaactgttc gatatcatcg acccgattgc tattgccgaa gtacgcgaag cactcactcg
    11701 tactctggcg actgaactgg cggatgagct gctggctatt tacaacgcga attaccagag
    11761 cgagtaccgt gttgagcatg aagatattgc aaaacgcact ctgcgaaatg cctgcctgcg
    11821 cttccttgct tttggtgaaa cgcatctggc tgacgtgctg gtgagcaagc aataccatga
    11881 agcaaacaat atgaccgatg cgctggcagc gctttctgcg gcggttgctg cacagctgcc
    11941 ttgccgtgac gcgctgatgc aggagtacga cgataagtgg catcaggacg gtctggtgat
    12001 ggataaatgg tttatcctgc aagccaccag cccggcggcg aatgtgctgg agacggtgcg
    12061 tggtctgttg cagcatcgct catttaccat gagcaacccg aaccgtattc gttcgttgat
    12121 tggtgcgttt gcgggcagca acccggcagc gttccatgcc gaagatggca gcggttacca
    12181 gttcctggtg gaaatgctta ccgacctcaa cagccgtaac ccgcaggtag cttcacgtct
    12241 gattgaaccg ctgattcgcc tgaaacgtta tgatgccaaa cgtcaggaga aaatgcgcgc
    12301 ggcgctggaa cagttgaaag ggctggaaaa tctctctggc gatctgtacg agaagatcac
    12361 caaagcactg gcttgatact tttgagccaa aatgcctgat gcgacgcttg acgcgtctta
    12421 tcaggcctac aactccgttg aaattgctgg ttttgtaggc tggataaggc gttcacgccg
    12481 catccggcag ccgtgcccaa ctgcctgatg cgatgcttga cgtgtcttat caggcctaca
    12541 agtccgttga aaatgctggt tttgtgcgcc acatccggca tattaaccat gacgccgaat
    12601 cagttgttca gaatgcccac gccgcattac ccgttgcaat acttccgctt ccagctccgc
    12661 taatcgcacc gaccctaatc gacgcggacg ggggatatcc actgtcaaat ccagaccaat
    12721 ttttccctct tcaattaaca acacccggtc agccattgct accgcttcgc tcacatcatg
    12781 cgtcaccaac agtacggtaa agccgtgctg ctgccaaagt gacacaatca aatcctgcat
    12841 ctcgagtcgc gttaaggcgt ccagtgcccc gagcggttca tcaagcaaca ataaacccgg
    12901 tcgatgaatc aacgctcttg ccagcgccac acgctgtttc tgcccgccag aaagtgcggc
    12961 aggccattcc ccggcgcgat tctccagtcc tactgcagcc aacgcccgac gcgcggcatc
    13021 gcgccactgg cctttaaggc ctaacccaac gttatcaatc accgatttcc acggcagcag
    13081 acgcgcatcc tgaaacatca ttcgcgtgtc ttcctgaatc tcagccagcg gtgtggtgcc
    13141 cgctaacaca tcgcctgcgg ttggcgtttc cagacctgcc agcaggcgca gcagggtact
    13201 tttgccacca ccgctgcggc ccaccaccgc cacaaactga cctgccggaa tatgtaaatc
    13261 cagttggttc aggacgatat tttccgcgta atgtttgctt actgcattga gcaacaatgg
    13321 tgtgccctgg ttcagacgag cagtattcat accgtggcct ccttcaaatg ataagccggg
    13381 ttccagcgca accagaggcg ctctaacagc tgcgcgctga cgtcagcaag tttgccgagc
    13441 agggcgtaaa gaataatagc gactaccacc acgtccgttt gcaaaaactc ccgcgcattc
    13501 atcgccagat aaccaatgcc tgaattggca gaaatggttt cggcaacaat cagcgtcagc
    13561 cacatcaggc ccaacgcaaa acgcacgccg accataattg agggcagggc accaggcagg
    13621 atcacatgga taaacagcgg tatgccggat aatccatagc tacgcgccat ttccaccaga
    13681 ccgcgatcga tattacggat gccatgccag gtgttgatat aaatggggaa tagcgtgccg
    13741 agcgccacca gaaagatttt tgcggattca tcaatgccaa accacaaaat caccagcggg
    13801 atcagcgcca gatgcggtac gttgcgcaac atctgaattg aggtatccag cagccgttct
    13861 ccccagcgtg acagcccgct aatcagtccc agaatcaagc ccagcgatcc gccaattgaa
    13921 aagccaatca gcgcccgcca ggaactgatc gccagatgct gccacaattc gccgctggcg
    13981 gagagcgtcc agaacgccgt caccactcct tccggtgaag gcaaaatacg cgtcgacagc
    14041 cagccaaccg aggaggccag ttgccacacc gccacgatgc ccaccggtaa aaaccagggg
    14101 gcaacgcgca ataaccactt cttcactggc gttgccatca tcgtgttcct tagctttgcg
    14161 cgactttacg ggggataaaa tcattcgcca ccgcttcgcc ttgcggattc agcggctgcg
    14221 gctggggaat ttccggaatg gcgacatcca gatgtgggaa cagcaactcg ccaacccgat
    14281 acgcttcttc cagatgcggg tagcccgaaa gcacaaagct gtcgatgcca agcgcggcgt
    14341 attcgttgat tcgcgcggcg accgtaggac catcgcccac cagcgccgtt ccggcaccgc
    14401 cgcgcactaa gccaacgccc gcccataaat tggggctgat ctccagattg tcgcgcttgc
    14461 cgttatgtag cgccgccatt cgctgttgtc ctacggaatc cgtccgggcg aatgcggcct
    14521 gtgctttggc gatagtttca tcatcaagat gcgagattaa ccgctcggcg gcctgccacg
    14581 cttcgtcgtt agtttcacga acaatcacat gcagacgaat accgaaacga attttgcgtc
    14641 catgcgcggc agctttcgcc cgcacttgtt cgatcttctc ttttaccagc tctggcggtt
    14701 cgccccaggt gaggtagaga tcaacttgtt ctgccgccag ctcctgggcg acatctgacg
    14761 atccgccaaa gtaaagcgga ggatacggct gttgaatcgg cgggaagagc agttttgctc
    14821 cccgcacatg aatatgttta ccgttgaaat cgacgctttc ccccagcaat aagcgccccg
    14881 ccagacctgg gtaaattccg ccgaggcttc gtagcgctcg ctatgatcaa ggaacacgcc
    14941 gtcgcctgcc aactcttgtg gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt
    15001 tgagagacgg tcaagcgtgg cggcctggcg agcggcaacg gtaggtgagg ttacgctggg
    15061 acgcagggcg acaagaaact tcagccgctg cgtcaccggg atcatcgacg ccgccaccag
    15121 ccacgcatct tcacacgatc gccccgttgg gatcagcaca ccggtatagc ccagacgatc
    15181 cgccgcttgc gcaatttgtt gcagatagcc gtgatcaacc gggcgtgaac cttcttccgt
    15241 tccaagataa tgcccgtcac catgggtcgg taaaaaccag aacatattca gactcataat
    15301 tgttttcctt ccaggtgagt gggctgccag atgcgctggc gaatatcaac ttttttcgac
    15361 accagacggt tttcataaaa cagatctgcc gtttgctgct gtaaggcggc aacttcagcg
    15421 ctcaccggtt tgatggtggt gggaggacga tgatccagat acgaggcaat caccggtgcc
    15481 ggtaagccca tcgtttttgc cagtaaagcg atgctttgct cgcgctggct gcgggttaac
    15541 gcatcggcct cactaaaggt tgccagtacg ccctgaataa aagcgccgtt tttttctgca
    15601 taggggcgag ctgccagata aaacgatcca gtttgattga gatcggtgcc gtctttcagc
    15661 acccgcacgc cgccctgtaa taacgcagcg gagtagtagg gatcccagat agcccaggcg
    15721 tcaacgttac cttgctggaa cgcggcgcgg gcatcagctg gcgtcagata agtgggctgg
    15781 atgtcagtaa acttaagtcc ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg
    15841 gaacctttct gaaaggcaac tttgtgacct ttaagatcgg ctacggtttt gatcgggctg
    15901 ttttctgcca ccagaatcac ttcggctttg ggcttcggtg gctcgacgcc cacatatatc
    15961 aaatcagccc cggcagcctg ggcaaagatt ggcggaatat ccccggtact gccgagatca
    16021 atactgccaa cgtttaacgc ttccaacatt tgcggacccg cggggaactc cacccaggag
    16081 atttttgatt gcggatagcg tttttccagt aactggtggc tttttgccaa taccatacca
    16141 atactgcctt tctgatagcc tatacgtaac gcttcaggcg aggattctgc agcaaccgca
    16201 aaagtagaga cgctaagcaa tcccgccagc gccagtttaa tgatgttacg catggacatt
    16261 acctcgcaga gacagaaggt caggaacctg aacatcgcgg cggtgcaatg cctgccagaa
    16321 agtttctagc gcggtatcaa gacgggtttg cagatttggc gtgaactggg gtttgtgatg
    16381 gtaatcaata acttgtgagt catcggcaaa cacgccgtgc aggagctcct gggctttcat
    16441 cgcacttaaa actggcttaa gggcataatc gaccgccagc agatgagcca ccgtaccgcc
    16501 cgtcgccagc ggtagcacca ctttgccttg caaagcgcgc tctggcagca ggtcgagcag
    16561 ggttttcagc gcgccggaat aggcggcttt atacacaggt gtagcgacta tcagtccatc
    16621 agcctgttgc agctgttcgg tgaaggtctt gagtgccgga ctatcgaaac gagcgtaaag
    16681 cagatcttcc ggggcgaagt tttgcagatt ccagtgataa acctctacat ccaggccatt
    16741 tagtttttcc cgcgcatatt ccagcaagga gctggagcga gaaggaaagc gaggactacc
    16801 cgccagggtg atgacacgca tactctctcc ttataaccaa ttgttctttt tttgttaaca
    16861 ttgataacaa ttcggtcagt ctgtcggaga gacaagaaaa tgccaaatat aaattttgtg
    16921 tatctttttc tgaaaataaa ctaaagaaag ggctttatat atcacgcata tttatttgtt
    16981 ggatagtcat tagatgttga atcaatgaat tactcgttca gttaatattt aaatattcat
    17041 gaaatctata aattaaagat ttgtcactta ttggatttag tatgcttgat ttaaatggcg
    17101 gtcaattatt gttattatta agtctaatgt catttaaag
//
LOCUS       DAQSWQ010000089        16913 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.089, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000089 DAQSWQ010000000
VERSION     DAQSWQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16913
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.089"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..177)
                     /locus_tag="R8G18_003077"
     CDS             complement(<1..177)
                     /locus_tag="R8G18_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEE9796708.1"
                     /translation="MSDLKTRLKEYEGTKEYQANLKYFRNGKFYPYADSLGCSTIGYG
                     HLIQAGEDFSTGITE"
     gene            259..648
                     /locus_tag="R8G18_003078"
     CDS             259..648
                     /locus_tag="R8G18_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796709.1"
                     /translation="MLSGEYAGFVYIFQFEDGSNYVGAKQIYQRVKDARKLKPTSKEN
                     GWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAILILHQMLKPECLNLAM
                     MSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            658..1236
                     /locus_tag="R8G18_003079"
     CDS             658..1236
                     /locus_tag="R8G18_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796710.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIYDFYKEANDGVRVIMSGIQGTFRISRQ"
     gene            1233..1544
                     /locus_tag="R8G18_003080"
     CDS             1233..1544
                     /locus_tag="R8G18_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796711.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            1541..1834
                     /locus_tag="R8G18_003081"
     CDS             1541..1834
                     /locus_tag="R8G18_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796712.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            1895..2689
                     /locus_tag="R8G18_003082"
     CDS             1895..2689
                     /locus_tag="R8G18_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEE9796713.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPNSFHINLLIFGDQFDSILRDF
                     PASYPLNDKLLHGHFQSFIDSYKNIDFIISGHLPISDNRNIQVFGGANHNLLCHFNYD
                     ELPSDKALIIEQDLISHKISLLNAYEGLINYLRAYYPEVEIYSPIL"
     gene            complement(2715..3170)
                     /locus_tag="R8G18_003083"
     CDS             complement(2715..3170)
                     /locus_tag="R8G18_003083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796714.1"
                     /translation="MDKKGIFLIIALLIFILLMLLLFAKLNFNVNTALAILSLILTVL
                     SGTDLVKKYTTVKSTNKSPIDMWMVFGIAISAVSMYGLYIDPQLSPLEGLTICIVSLI
                     LFVSVLVSALLNYKKIKKPDVREIIKQSHSVFRENEETITITTTIKKAP"
     gene            3205..3930
                     /locus_tag="R8G18_003084"
     CDS             3205..3930
                     /locus_tag="R8G18_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEE9796715.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIY
                     RDEWNGTKVICESVLVDDDGTNL"
     gene            4423..5169
                     /gene="mipA"
                     /locus_tag="R8G18_003085"
     CDS             4423..5169
                     /gene="mipA"
                     /locus_tag="R8G18_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HEE9796716.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            5259..6113
                     /gene="yeaE"
                     /locus_tag="R8G18_003086"
     CDS             5259..6113
                     /gene="yeaE"
                     /locus_tag="R8G18_003086"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HEE9796717.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(6164..7048)
                     /locus_tag="R8G18_003087"
     CDS             complement(6164..7048)
                     /locus_tag="R8G18_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HEE9796718.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(7132..8127)
                     /gene="gapA"
                     /locus_tag="R8G18_003088"
     CDS             complement(7132..8127)
                     /gene="gapA"
                     /locus_tag="R8G18_003088"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEE9796719.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8469..8882
                     /gene="msrB"
                     /locus_tag="R8G18_003089"
     CDS             8469..8882
                     /gene="msrB"
                     /locus_tag="R8G18_003089"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEE9796720.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8924..9196
                     /locus_tag="R8G18_003090"
     CDS             8924..9196
                     /locus_tag="R8G18_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HEE9796721.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(9357..9998)
                     /gene="pncA"
                     /locus_tag="R8G18_003091"
     CDS             complement(9357..9998)
                     /gene="pncA"
                     /locus_tag="R8G18_003091"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEE9796722.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(10009..11025)
                     /gene="ansA"
                     /locus_tag="R8G18_003092"
     CDS             complement(10009..11025)
                     /gene="ansA"
                     /locus_tag="R8G18_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEE9796723.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(11192..13048)
                     /gene="sppA"
                     /locus_tag="R8G18_003093"
     CDS             complement(11192..13048)
                     /gene="sppA"
                     /locus_tag="R8G18_003093"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEE9796724.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            13209..13760
                     /locus_tag="R8G18_003094"
     CDS             13209..13760
                     /locus_tag="R8G18_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HEE9796725.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            13877..14920
                     /gene="selD"
                     /locus_tag="R8G18_003095"
     CDS             13877..14920
                     /gene="selD"
                     /locus_tag="R8G18_003095"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEE9796726.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            14925..16886
                     /gene="topB"
                     /locus_tag="R8G18_003096"
     CDS             14925..16886
                     /gene="topB"
                     /locus_tag="R8G18_003096"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEE9796727.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         4583 a         3805 c         4211 g         4314 t
ORIGIN      
        1 ttcagtaatg ccagtactaa aatcttctcc cgcctgaatt aagtgaccat atccaatagt
       61 actacatcct aaactatcag catatggata gaatttccca tttctgaagt acttcaaatt
      121 tgcctgatac tctttagtac cttcatattc ttttaatcgt gtttttaaat cgctcattta
      181 ctaaatacct ataatgaatt tataggaata tttatgatga gtgaatggaa atactcggat
      241 gattggagcg aggaggaaat gttatccggt gaatatgctg gtttcgttta tatctttcag
      301 tttgaggatg gttctaatta tgtcggtgct aagcaaatct atcagcgggt taaggatgcg
      361 agaaagctaa agcccacttc aaaggaaaat ggctggcgtg agtacctcag tagttccaat
      421 gtcgttaaaa acaaaattga aaatggtgaa cagtactcaa aaacgattct atggtgcttc
      481 gcaactatgc gagaggtaat gattgtagag gcaatactga tccttcatca gatgcttaaa
      541 ccggaatgtt tgaatctggc aatgatgagt aagattagat caccaaatgc aaaggataaa
      601 aaaagactac tcggaatagt acaggaacta ttggagtact tgaattgagg taaatatatg
      661 gcaagtggaa taaatgggat tggtagtata aggaactata tacaaacgca gggagttaga
      721 ttaaataatc aattccagaa tgaaataatt aaacgttcca agctactatc ccaaagaatg
      781 cagggtgatt tgaataatag cgttgaccgt ggggcagtgg gctttactca acgttccatc
      841 cttttcttct atagaaaggt tgggagaaat tcagttacat cgaccatcat ggttaaagat
      901 attcaggcta agtaccttta tcaagtactg gtacagccaa aggcaattga taagtttata
      961 cctacttcat ctacaagatt aactaagcag ggcaacattt caggactgaa aaagaatctt
     1021 ttaagtgaac gatataaggt agtgaagggt aagaatggta aagaacgatt aattgatacc
     1081 gcaaagaaag ataccaagaa gaaaaccaaa cgtgtaattg gcttacgtga gaagaaacaa
     1141 cgtcgcctta tctatgactt ctataaggaa gccaatgatg gtgtaagggt aattatgagc
     1201 ggtattcagg gaacattcag gataagtaga caatgaattt tgagcaacat tatggtgatt
     1261 taataagcaa gattaagcta aatggcatga caccagatgg taatagtatg ccaattaaca
     1321 aatgttttct tgataagaaa tttcaaaagg tcgttaagaa aagtaattat cagattgata
     1381 gctatatgaa cgttatttat gagaagaaga aagatttcaa agttggtgaa gtacttgaat
     1441 gggaattaca gggtgaaaag atgcctgtat ttctaattga gagtaaaaag ctctttgtga
     1501 agggtaaaca tttttgggta tatgccgtgg ggattattga atgattagta tattgattga
     1561 gtttctgaag tcaggattgg attttttcat taagaagaaa actattgaac aggaagtaaa
     1621 gaaaacgaat gtagaagggc agattgaaac gaataaagaa gagattgaaa aggtaacatt
     1681 ccactggcgt aatgccctcg gttttgtact aacccttatc attctctata actggatcat
     1741 agtaccagta cttgatgctt ttggaattat tgtgatccaa gttccattag gacaactttt
     1801 gcaagtgcta ttgattatgg ttggtggaag ctgaaagatt tttcctttaa tatgtgtttt
     1861 gagttaataa aatagcaatc taaggatttg atttatgagt gatgcaatta ctacagcagc
     1921 aataaaaaaa ttgtatggac tatccgctgg gcaatgtaat atttgccgaa cttctctttt
     1981 taaagagcag gttcacatcg gacaaatggc tcatgtaata gccaaaagcc caaaaggacc
     2041 aaggggcgat gacctgttag caaatgacaa ttcttatgaa aatcttatcc tgctttgtgc
     2101 aaatgatcat attcttgttg atagagatcc attcacttat acagtagaaa aattgcatca
     2161 aatcaaaagt gaacatgagg cttatataca gggtgcaacc gataatacat tattttcaga
     2221 taaaaaaaga cagtcagatg tggacttttt aaacgcttac ttccacttta caccgtttgc
     2281 gagggtacgt tcattagttg aaactttacc taattctttt cacatcaatc ttcttatttt
     2341 tggagatcag tttgatagta ttttaaggga ttttccagcg agctatcctt taaatgataa
     2401 attactccac ggtcatttcc aaagttttat tgattcgtat aaaaatattg attttatcat
     2461 atctggacat ttgccgattt ccgataatag aaacattcag gtgtttggtg gtgcgaatca
     2521 taatcttctt tgtcatttca attatgatga gctaccatcc gataaagcac tgataataga
     2581 acaagatttg atttcacata aaatatctct tcttaatgct tatgaaggtc ttataaatta
     2641 ccttagagct tattaccctg aggtggaaat ttactctcct attctatgag tgttatgaaa
     2701 tatataaaag ccccttatgg ggcttttttt atggttgttg taatagtaat ggtttcttcg
     2761 ttttctctaa aaaccgaatg gctttgtttt attatttccc taacatctgg tttctttatt
     2821 tttttgtaat ttaataaagc cgaaactaaa acagaaacaa aaagtataag tgatactata
     2881 caaatagtta aaccttctaa aggtgatagt tgggggtcta tatataatcc atacatagaa
     2941 acagcggaga tagcaatacc gaaaaccatc cacatatcta tagggctttt atttgtcgat
     3001 ttgaccgtag tgtacttttt gactaagtcc gtacctgata gcactgttaa aattaatgat
     3061 aatatagcga gggcagtatt tacattaaaa tttagcttag cgaataaaag caacattagt
     3121 aaaataaaaa taagtaacgc tatgattaaa aatattcctt tcttgtccat aaataccccg
     3181 tcgtattttt aaaaaaggta tcttatggca aggataagta aaaaggaatc tgctaatcac
     3241 aataaagtga tggaattaat acattcagat aagctcctaa cacaagaaga tagggaattc
     3301 atattcacca attatagggg ggatgggata ggggctacag gtgccttctt cacacctgaa
     3361 attttagcat gggattttgt acttgatgct gactgtactg gtgactgtat tgaactatgt
     3421 gctggtatcg gtcgcctcag ctactaccag tacttaagga ataaaccaac gcatattaca
     3481 tgcgtggaac tcaatcctga gtacgtaatg atcgggaaga gggtactacc agaggcagaa
     3541 tggatcactg gtaacgcact acagtactca ccagaccgtt tctatgatgt tgcctatggt
     3601 aatccaccct ttgggaagat cagtacctca aaagcgtata caggccgtta taagggtagt
     3661 gaatttgaat acaaggtgat tgaacatgca agtacctttg cgtcctatgg tgcatggata
     3721 gtaccgcaag gttccgcagg ctttgtctac agtggggtac attgttatga gcgtagagaa
     3781 tcatgtaagt accagaagtt tgtgaaggat actggctatg agctacatcc aggtgtaggc
     3841 atcgatacaa gtatctacag ggatgaatgg aatggtacta aggtgatatg cgaatcggtg
     3901 ttagtagatg atgacggtac aaatctgtaa ctaaattagt taagaaatca taaagatagt
     3961 agtacttgca aatgttccca ttgatgaagt tatgcgtcct ttcgtttttt agcacaggtt
     4021 aagagccgtt catatgaata gtagaggctc ccggaagagg taaactgaac gacgtctctt
     4081 gctaaaacac agcaactctc atgccatttt atcgacgatg agaacaacgt gcccggtgat
     4141 acaccttcaa aattaagcgt agatggcaaa tggaaaactt gcctgcacgg ctttcttaat
     4201 ttcaatggca tatcaacaac tcatccataa attttgcata attaatgtaa agacaaggct
     4261 cgccagtaac gctaaattca tttggctgta agcgcggtgt catccgcgtc aggaaaatta
     4321 aacagttact ttaaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa
     4381 actttgtcgc ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa actcaaactt
     4441 ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga aggtaaattt
     4501 tccctgggcg caggcgtcgg tgtcgttgaa cacccatata aagattacga taccgatgtt
     4561 tacccagtac cggtaatcaa ctatgaaggc gataacttct ggttccgtgg cttaggtggt
     4621 ggttactacc tgtggaatga cgcaacggac aaactttcaa ttaccgctta ttggtcgccg
     4681 ctttacttca aagcgaaaga cagtggcgat caccaaatgc gtcacctgga tgaccgtaag
     4741 agcaccatga tggctggtct gtcttatgct cactttaccc agtacggtta cctgcgtacc
     4801 accctggctg gcgataccct ggataacagc aacggcattg tctgggatat ggcctggttg
     4861 tatcgttaca ccaacggcgg cctgacagtg accccgggta tcggtgtgca gtggaacagc
     4921 gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg
     4981 cgtggctata acccgaacga cagctggagc ccttacctgg agctgagcgc cagctacaac
     5041 ttcctcggcg actggagtgt ttacggtacc gcgcgctata cccgtctgtc tgatgaagtt
     5101 actgacagcc cgatggtgga taaatcctgg actggcctga tttctaccgg gatcacctac
     5161 aaattctgat aatgcatctt tccagggcat tttgtgcata aaaacagggc gtcacgccct
     5221 gttttgcatt acggtggtgc aatacctggg aggaaacaat gcaacaaaaa atgattcaat
     5281 ttagtggcga tgtctcactg ccagccatag ggcagggaac atggtatatg ggcgaagatg
     5341 ccagtcagcg caaaacagaa gttgctgcac tacgcgcggg cattgagctc ggtttaaccc
     5401 tcattgatac cgccgaaatg tatgccgatg gcggtgccga aaaggtggtt ggggaagcat
     5461 taaccggtct gcgtgataac gtctttctcg tctctaaagt ctatccgtgg aatgctggcg
     5521 ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg tctcaatact gattatctcg
     5581 atctctactt attacacagg tctggcagtt tcgcttttga agagactgtt gcagcgatgg
     5641 aaaaattgat cgcccaggga aaaatccgcc gctggggcgt ttctaacctt gattatgctg
     5701 atatgcagga actctggcag ctgccggggg gaaatcagtg tgccactaat caggtgcttt
     5761 accatctcgg ttcacgaggg attgagtacg atctactccc ctggtgccag caacagcaga
     5821 tgccggtgat ggcttacagt ccattagccc aggccgggcg gttgcgcaat ggactgttaa
     5881 aaaacgcggt agtcaacgaa attgcacatg ctcacaatat cagctcggca caagtattgt
     5941 tggcgtgggt aatcagccat cagggtgtga tggcgattcc aaaagcggcc acgattgccc
     6001 atgttcaaca gaatgcggct gtgcttgagg tcgaactttc gtcagcggaa ttaaccatgc
     6061 tggataaagc gtatccggca ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat
     6121 acgtgccgga tgcatcgcac ccggcacaca acattaacgc aaattaacgt ttcgcaacgc
     6181 gaatggattg cgccagatgt gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt
     6241 aagccgtttc tacacaaaca aatgttttgt agccatcatc cggcatatcg cccatgctaa
     6301 ttgaaagcgc cggtcccggg ttccagccta caacgttcag atgatgctgg tggcctacgg
     6361 cgataatacg attcagcgct tcatcattaa tcacgctgca atcttgcgga ttcagataca
     6421 cgcgatcggt acggtcaggg aaggtctgaa taccgtcggt cagtacatct tctttcgcgt
     6481 cattcacttt atcaatgaag cgatcgccca gcccactgac gcttacctta gcgatatcac
     6541 ccacgttaaa gtaggtatgc agggcagagg tggtttcaaa ttcgccatgt gattcaagat
     6601 cgatttcaca ggttttaccc acgcggaaat gcgctaacag cgtaaagtcg tgcggacaga
     6661 attttttcgt ctcttcgctt tgcgtcaatt caaaagtcag cgctacgcca tcagcatctt
     6721 cattatgtga tttcagcgtc cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt
     6781 gttgtgccgc cggaccaaac cacggccagc aaaccggtac gccaccgcga atagcgacgc
     6841 catttttgaa cggtgtgttg ttgctcaacc acagaacttc ttcttcaccc gcaggtttcc
     6901 acgagagaag gtgtgcgccc tgtaatgcaa aagaggcttt tacttgggga tgatcgacca
     6961 caatgaggtc cagttcatcc agtttacgac gggagaggac aggggagatt tgttcgatga
     7021 ccggaagggc aaaaattttc ttaatcatga cgcagtcctt taacttcatt ttatcaggta
     7081 aaaaaaagag cgaccgaagt cgctcttttt agatcacagt ttcatctcaa cttatttgga
     7141 gatgtgagcg atcaggtcca gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga
     7201 taccagtttc acgaagttgt cgttcagagc gataccagct ttagcatcga acacggaagt
     7261 gcaaacttcg ccgttgaaat cggtagatac tacgtcatct tcggtgtagc ccagaacgcc
     7321 tttcatttcg ccttcagcag cggctttaac ggcagctttg atctgctcgt aagttgcagc
     7381 tttttccaga cgaacggtca ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc
     7441 cataccagtc agtttgccat tcagttctgg cagtacttta cctacagctt tagcagcacc
     7501 ggtagaggac gggatgatgt tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga
     7561 cgggccatca acggttttct gagtagcggt agtagcgtga acggtggtca tcagaccttc
     7621 gatgatgccg aagttatcgt tgataacttt agccagcgga gccaggcagt tggtggtgca
     7681 ggaagcgttg gaaacgatgt cctggccagc atatttgtcg aagttagcgc ctttaacgaa
     7741 catcggagtg ttgtctttag acggaccagt cataaccact ttcttcgcac cagcggtgat
     7801 gtgtttacga gcagtttcgt cagtcaggaa cagaccagtt gcttcagcga caacgtcaac
     7861 accaacttcg tcccatttca ggttagccgg atcacgttca gcggtaacac ggattttttt
     7921 accgttaacg atcagatgac cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt
     7981 ggagtcatat ttcagcatgt atgccatgta atcagcgtct aacaggtcgt tgattgcaac
     8041 gatctcgatg tcagaacgtt tctgagcagc acggaaaaca atgcgaccga tacggccaaa
     8101 accgttgata cctactttga tagtcatata ttccaccagc tatttgttag tgaataaaag
     8161 gttgcctgta aaattacaaa aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc
     8221 gacaaatcaa tcctgtgcct aagcattacg cgactgactc gcctcactct tcctttgggc
     8281 ttgaaaccac atggggtcgg cgccccgaat tttaaagggc aattacgata aaaatgtgat
     8341 tttcatcacg atttaggtgc agccatttcg tcgagatcaa gtttaggaca aaattttgcc
     8401 agttggtgtt aatgttttgt tagaatcagt cacgtaatgt gagcacgatt aaagtgagat
     8461 gtgagcaaat ggctaataaa ccttcggcag aagaactgaa aaaaaatttg tccgagatgc
     8521 agttttacgt gacgcagaat catgggacag agccgccatt tacgggtcgt ttactgcata
     8581 acaagcgtga cggcgtatat cactgtttga tctgcgatgc cccgctgttt cattcccaaa
     8641 ccaagtatga ttccggctgt ggttggccca gtttctacga accggtgagt gaagaatcca
     8701 ttcgttatat caaagacttg tcacatggaa tgcagcgcat agaaattcgt tgcggtaact
     8761 gtgatgccca tctggggcat gtcttccctg acgggccgca gccaacgggc gaacgttatt
     8821 gtgttaactc tgcttcttta cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt
     8881 gaaaaaacga ttcagcaact tattccacag gagcggaaaa attatgaatc ttgatgacat
     8941 tatcaacagc atgacgcctg aggtatacca gcgtttgtcg accgccgttg aactggggaa
     9001 atggcccgat ggcgttgcgt tgaccgagga acaaaaagcg aactgcctgc aactggtgat
     9061 gctgtggcaa gctcgccata ataccgaagc acagcatatg acaattgaca caaatggtca
     9121 gatggtcatg aagagcaaac agcagttaaa agaagatttt ggtatctcag caaagcctat
     9181 tgctatgttt aagtgaatga gttatctagt atttaagcct gttattaagg gagggcaaat
     9241 atacttgtta ggacgtcgtg ttttagctgc aagtaggtta aatgcaacac tgacaagtat
     9301 tatctggcct acaatgtcat tgaggaaatg taggccagag atgaattcaa ctaatattac
     9361 ccctgtgtct cttcccagtc tgccagcgta tatagcgttg ccccagctgc tgacatctcc
     9421 ataaacgcgt gcgcactgtc ctggggctgg atattcacgc cacgacaacc atcggtaatc
     9481 acatttacct tataacctaa ctgtaacgcg tccagcacgg taaacttcac gcaatagtca
     9541 gtagccaggc ccataacgat caattcatcg atttcatgat cgcgtaacca gtcatcgagc
     9601 gaggttttct gccgacggcc gttatcaaaa aaggcactgt aactgtcaac taaaggattt
     9661 tcgcctttat ggaacaccgc tgcgatcgct ttttggttca gtaacggatg taattgtgcg
     9721 ccttcactgt tctgcacaca gtgatctggc cagaaggttt gtggcaaacc gtcgagttgg
     9781 cctggcgtat aaggctctac accgtgctga ctggcaaaac tgccgtgatt cgccgggtgc
     9841 cagtcctgac tggcgataac cgcttcaccg cgcgactggc accagtcaat caggcggtta
     9901 gcgacatcca ccgtactgtc accttccggc acggcgagcg cgccaccagc acagaaatca
     9961 ttttgtaaat cgaccagtaa cagggcgcga gggggcatta cagtctcctt aatcatccgg
    10021 cgtcagttcg ccgcgcaggt tttggctcat ggccttgcga atggtttcag tatccagttc
    10081 ctggctcagc aggtaatgca gtttggttag cgtggcttct acagtcatat ctgcaccgcc
    10141 aataacgccg gcatgggcga gggcgttacc ggtggcataa ccacccatat tcactttacc
    10201 ggacatacat tgtgtcaggt tgaccaccac aataccgcga tcgctggctt cttgtaattc
    10261 ctgcaggaag gctttgtttt gtggcgcatt acccacgcca taggagcgca gaatcaatgc
    10321 tttcaccggt tggcgcagaa aattgcgcac gacgtcagca gaaatccctg gataaatcgt
    10381 cactacgcca attggttgtg gcgtgattgg atgaacgatt aattcccctt caccgtgcgg
    10441 ggcgggtggc gtattcaaac gacgaatatg gataccagct tccagtaacg gaggaaggtt
    10501 tggagaggca aacgcatcaa aaccatcggc atgggctttg gtagtgcggt tgccgcgata
    10561 caatcggtta ttgaaaaaga gcgttacttc gttgatcgga taattcgccg caacgtacaa
    10621 cgcattcagc agattaattt gtccgtcaga gcgtaactca gccagcggga tttgtgaccc
    10681 tgtcacaatg accggtttac cgagattctc gagcatgaac gacagcgcag aggcagtata
    10741 cgccatcgtg tcggtgccgt gcaggatgac aaaaccatca tagtcgtcat agtgcgcttt
    10801 aatatcttca gcaatatgct gccagtcttc tggcgtcata tcagaagaat ccatcagcgg
    10861 cgtatattca tgaatggtga aatctggcat ctccgggcga tggaattccg gcatcagcgc
    10921 cagttggcgt tgtagatgac ctgacaccgg aatataaccc tgctcggaac gctgcatccc
    10981 gatggtcccg cccgtgtagg cgacgtaaat tgatttcttt tgcatgatat tgatatgaat
    11041 gatatcgaaa gagcaaaagt atatacgtga ggcaggagga gcgcacctga tactatgcgg
    11101 ataaggaaat atggggcgag acagcgggaa gaggattcgt cgacgtaaaa aataatgtgg
    11161 ccgacgatct gtcggccact cagtacaaga cttaacgcac gttggcgcag gtcaggcaaa
    11221 acgcataacg gttttgtggg tcgttaaacg cggccagctt gtcgctttca cttttaacgg
    11281 tagaggctac cgaggcaagc ggtgcaggta acatggcctg gaacgcatct ggcaacattg
    11341 cccggacaga accagacatg ttgtccatca ctttgtcgaa gaaggtcggt tcatcaacgt
    11401 agtattccag atgccactgt ttcacttttg ccagctctgc tgctttggcg accgcatcat
    11461 cgaaatcccc gagactatcg accagcccgt tagcttttgc atcctgaccg gtccagacgt
    11521 ggccctgggc gattttatca atctgctccg gcgtcgaatg acgcgcatca gcaaccagcg
    11581 tgataaagcg tttatagcca ttctcaatgc ttaattgcat catctgctgc gcttccggcg
    11641 gcagtgccct ggtgatagaa acatccgcca gcggtgaagt tgagacacca tcagtatgaa
    11701 caccaatcga atccagacta ttttctacgg tggtgatcac gccgaagata ccgatagaac
    11761 cggtcagggt gctggggtta gccacaatgt aattagctgg cgtggaaatc cagtaaccac
    11821 cagatgccgc catgccgccc atcgatacaa ccacaggctt acccgctgcc cgggctgctg
    11881 ccagttcagc gcgaatcact tcagacgcgg taacgctgcc gcctgggcta ttaacacgca
    11941 ggacaatcgc tttcactttc gggtcaaggc gagcgtcgcg gatttgtgcc gcagtggtat
    12001 caccgccaac attcccctga gtttcctcgc catccataat tgcgccatta gcgaagacga
    12061 caccgatgct gtcaccggta tctgccggcg ttttcaatgc gtaatcgtaa taactgatgg
    12121 cgcgataatt tttatcagtc ttactccagc cgaactcttt ggttagagct ttttcgattt
    12181 cggcactcga tgccagtgca tcgaccagct tgttttccag tgcatatttc gcggtatcgc
    12241 caccggtttt ggttaaaccc tcaagcaacc cttgcgcgcc agggaatacc tgctgagcag
    12301 ggatctgccg gttagcggca acagtattca gatagttttg ccacagctca ccaatccagc
    12361 ggctgtcagc ttcgcgggct gccggtgaca tgtcatcacg aataaacggt tcaacggcag
    12421 atttatacgt acccacgcgg aacacatggg tggaaacttt cagcttatcc agcaacgatt
    12481 tgtagtacag accgttggta gcaaaaccgt gcaaatccac cacgccttgc ggtgacagcc
    12541 aaattttatt ggcgaaactg gcgagataat attgcccctg gctgtagttc tcgccaacgg
    12601 cataaaccgg tttcccgctg tcacgaaact ctttcagggc tttgccgatg tactgcatag
    12661 acggctggtc gccgcctgcg aagtttttca gatccatcac gataccggtg atattgcggt
    12721 cgtccttcgc ctggcgaatg gtgttgacga tatcaaacag tgagttttcc tgcagacgat
    12781 cggaactggc accaagcagc tggcggctta atttactaaa ccgctgagaa ctgtcgggtt
    12841 tatcgacgat cacaccagaa atgtccagca gcagtgcgcc acgactggcc gtttctttcg
    12901 aatcaccacc actgacctgc atccagatcc ccacaccaac cagtacgagg aaaataaaga
    12961 acaggttaag taccatttca cggacgaaat tcagcagacg ccacgtccat ttaaaaaatc
    13021 cggcaataaa tcgccaaagg gttcgcatgt attctcccaa cttaaggtca cacctgtcac
    13081 aggcgcaata gatgtggtta tcgtaatgac ccaaccggca cttgtcagca ggaattgcgg
    13141 actgtgctgt aacaaaatcc actctcgtgt taattttgtg aataaatatc acgacaggag
    13201 ttaatcaaat ggatgcactc gaactattga tcaatcgccg tagcgcctcc cgcctggctg
    13261 aacccgcgcc aacgggtgaa caactgcaaa acatcctgcg tgcgggtatg cgtgcgccgg
    13321 accataagtc catgcaaccg tggcattttt ttgtgattga aggggaaggg cgcgagcgtt
    13381 tcagcgccgt actggaacag ggggcgattg ctgccggtag tgatgacaaa gctatcgaca
    13441 aagcccgtaa tgcgccgttc cgcgcaccgc tcatcatcac ggtggtggcg aaatgcgaag
    13501 aaaatcataa agtcccgcga tgggaacagg aaatgtctgc cggatgcgcg gtcatggcga
    13561 tgcaaatggc agccgttgcc cagggatttg gcggcatctg gcgcagtggc gcattaactg
    13621 aaagtccggt agtgcgtgaa gcattcggtt gccgtgagca ggataaaatt gtcggttttc
    13681 tctacctcgg cacgccgcag ttgaaagcat ctacgtcgat taacgtcccg gacccgacgc
    13741 cgtttgtaac ttatttctga taattgtcgc gaaactgtct ggattgtgag caaaggcgca
    13801 ggaattcaga cactctcact tatcacttca cggaatgagg gttaccatac ccggattgca
    13861 ttgacaggag atgtccatga gcgagaactc gattcgtttg acccaataca gccacggagc
    13921 tggttgcggc tgtaaaattt ccccaaaagt gttggaaacc atcctgcaca gtgagcaggc
    13981 gaagtttgtt gatccgaatt tgcttgtggg taatgagacc cgcgacgatg cggcggtgta
    14041 cgatctgggc aatggcacca gcgttatcag taccaccgac ttctttatgc cgatcgttga
    14101 taatcctttc gattttggcc gcattgcggc gactaacgcc atcagcgata tcttcgcgat
    14161 ggggggcaaa ccgattatgg cgattgcgat cctcggctgg ccgattaaca aactttcccc
    14221 ggaaattgcc cgcgaagtaa ccgaaggtgg acgctatgca tgccgtcagg cgggtattgc
    14281 gctggctggc ggtcactcca tcgatgcgcc ggagccgatt tttggtctgg cggtaacggg
    14341 gatcgtaccg accgagcggg tgaagaaaaa cagtaccgca caagccggat gcaaactgtt
    14401 cctgacgaaa ccgctgggga tcggcgttct taccacggct gagaaaaaat cactgctgaa
    14461 accagaacat cagggactag cgacggaagt gatgtgccgg atgaacatcg caggcgcgtc
    14521 ctttgccaac atcgaaggcg taaaagcgat gaccgacgtt acgggttttg gtctgctggg
    14581 ccatttgagc gaaatgtgtc agggcgcagg tgtgcaggcg cgcgttgact atgacgcgat
    14641 cccaaaattg ccgggggttg aagagtacat taagttgggc gcagtgcctg gcggcaccga
    14701 acgtaacttt gccagctacg gtcatctgat gggtgaaatg ccgcgtgaag tgcgcgattt
    14761 gctgtgcgat ccgcaaactt ctggcggttt gctgctggcg gtcatgccgg aagcagaaaa
    14821 tgaggtcaaa gctacagccg ccgagtttgg cattgaactg acggcaattg gcgaactggt
    14881 gccagcgcgc ggcggtcgtg ccatggttga gattcgttaa ttcaatgcgg ttgtttattg
    14941 ccgaaaaacc gagtctggcg cgcgccattg ctgatgtcct gcccaaaccg caccggaaag
    15001 gcgatggctt tatcgagtgc ggtaatggtc aggtggtgac ctggtgtatc ggtcacctgc
    15061 ttgagcaggc gcagccagac gcctacgaca gccgctatgc gcgctggaat cttgcggatt
    15121 tgccgattgt cccggaaaag tggcaattac agccccgacc ctccgtgacc aaacaactta
    15181 acgtcatcaa acgcttcctg catgaagcca gcgaaatcgt tcacgccggg gacccggatc
    15241 gtgaagggca actgctggtg gatgaagtgc tggactatct gcaactggca ccggaaaagc
    15301 gccagcaggt gcagcgttgc ttgataaacg acctgaaccc gcaggcggtt gagcgggcgc
    15361 tcgaccgtct tcggtccaac agtgagtttg tgccattgtg cgtttctgcg ctggcgcgag
    15421 cgcgtgccga ctggctgtac ggcatcaata tgacccgtgc gtataccatt ctcggtcgca
    15481 atgccggtta tcagggcgta ctttccgtgg gacgcgtgca gacacccgtg ctcgggctgg
    15541 tggtgcgccg cgatgaagag attgaaaact tcgtggcgaa agacttcttt gaagtcaaag
    15601 cacatatcgt gacacctgcc gatgagcggt ttaccgctat ctggcaaccg agcgaagcgt
    15661 gtgaaccgta ccaggatgaa gaagggcgct tgttacatcg tccactggcg gagcatgtgg
    15721 ttaaccgcat tagtggtcaa ccagcaattg tcaccagcta taacgataaa cgggaatcag
    15781 aatccgcgcc gctgcctttt tcgctttcgg cgttgcagat tgaagcggca aaacgctttg
    15841 gtctgagcgc gcagaacgtg cttgatatct gccagaagct gtacgaaacg cacaagctaa
    15901 tcacttatcc gcgttccgat tgtcgctatt tgccagaaga acattttgcc ggacgccacg
    15961 cggtgatgaa tgccatcagc gttcatgcac cagatctgtt gctgcagcca gtggtagatc
    16021 cagatatacg caaccgctgt tgggatgaca aaaaggtcga tgcacaccac gccatcatcc
    16081 cgaccgcacg gagttcagcc atcaacctga cggagaacga agcgaaggtc tataacctga
    16141 ttgcccgtca gtacttgatg cagttctgcc cggatgtggt gttccgcaag tgtgttatcg
    16201 aactggacat tgccaaaggc aaatttgtcg ctaaagcgcg ttttcttgct gaagcaggct
    16261 ggcgcacgct gttaggcagc aaagagcgcg atgaagaaaa cgacggtacg ccattgcctg
    16321 tggtggcgaa aggcgatgag ttgctgtgtg aaaaaggtga agtggtagag cggcaaaccc
    16381 agccgccgcg ccattttacc gatgcaacac tgctttcggc gatgaccggg atcgcgcgct
    16441 ttgtgcagga caaagatctg aaaaagatcc ttcgtgcgac cgatggtctg gggacagaag
    16501 caacccgtgc cgggattatt gaactgctgt tcaagcgtgg ttttctgacc aaaaaagggc
    16561 gctatatcca ctctaccgac gccggaaaag cgttattcca ttcgctgcca gaaatggcga
    16621 cgcgaccgga catgaccgcg cactgggaat cggtgctgac gcaaatcagc gaaaagcagt
    16681 gtcgctatca ggactttatg cagccgctgg tggggacgct atatcagctt attgatcaag
    16741 ccaaacgtac gccggtgcgg cagtttcgcg gcattgtggc tccgggcagt ggtggcagtg
    16801 ctgataagaa aaaggctgca ccgcgtaaac gtagtgcgaa aaaaagtccg ccagcagatg
    16861 aagtcggaag cggggcgata gcgtaagcga gtgaatcttt cgtgctattc gag
//
LOCUS       DAQSWQ010000090        16400 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.090, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000090 DAQSWQ010000000
VERSION     DAQSWQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.090"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="R8G18_003097"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="R8G18_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HEE9796728.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="R8G18_003098"
     CDS             complement(2380..2820)
                     /locus_tag="R8G18_003098"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEE9796729.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="R8G18_003099"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="R8G18_003099"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEE9796730.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="R8G18_003100"
     CDS             3538..4236
                     /locus_tag="R8G18_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEE9796731.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="R8G18_003101"
     CDS             4465..5673
                     /locus_tag="R8G18_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9796732.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="R8G18_003102"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="R8G18_003102"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HEE9796733.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="R8G18_003103"
     tRNA            7780..7856
                     /locus_tag="R8G18_003103"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="R8G18_003104"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="R8G18_003104"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="R8G18_003105"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="R8G18_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HEE9796734.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="R8G18_003106"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="R8G18_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HEE9796735.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="R8G18_003107"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="R8G18_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HEE9796736.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="R8G18_003108"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="R8G18_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEE9796737.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14610
                     /gene="dppF"
                     /locus_tag="R8G18_003109"
     CDS             13606..14610
                     /gene="dppF"
                     /locus_tag="R8G18_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HEE9796738.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14640..15911)
                     /locus_tag="R8G18_003110"
     CDS             complement(14640..15911)
                     /locus_tag="R8G18_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEE9796739.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3900 a         4094 c         4409 g         3997 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccggacga tcgccgcgag cgcatcaagc tcagcggtga actaccaagc
    14461 ccactgaatc caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    14521 tgcacccagt tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct
    14581 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    14641 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc
    14701 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    14761 atgaggcacg acacaatacc atataacggg cttcccaact gaaagaacac cagcaccgat
    14821 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    14881 agcacgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    14941 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    15001 atattaagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagc
    15061 gccagtgctg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    15121 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    15181 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    15241 ttcattgggt taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc
    15301 aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    15361 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    15421 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    15481 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    15541 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    15601 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    15661 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    15721 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    15781 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    15841 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    15901 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    15961 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    16021 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    16081 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    16141 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg
    16201 caagccgaaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    16261 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    16321 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    16381 ggctatatga cgctcgcaga
//
LOCUS       DAQSWQ010000091        16268 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.092, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000091 DAQSWQ010000000
VERSION     DAQSWQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.092"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(161..976)
                     /locus_tag="R8G18_003111"
     CDS             complement(161..976)
                     /locus_tag="R8G18_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796740.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(1219..2268)
                     /gene="yahK"
                     /locus_tag="R8G18_003112"
     CDS             complement(1219..2268)
                     /gene="yahK"
                     /locus_tag="R8G18_003112"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HEE9796741.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(2645..4027)
                     /locus_tag="R8G18_003113"
     CDS             complement(2645..4027)
                     /locus_tag="R8G18_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEE9796742.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(4037..4987)
                     /locus_tag="R8G18_003114"
     CDS             complement(4037..4987)
                     /locus_tag="R8G18_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HEE9796743.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(5130..6548)
                     /locus_tag="R8G18_003115"
     CDS             complement(5130..6548)
                     /locus_tag="R8G18_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEE9796744.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(6548..8095)
                     /gene="fdrA"
                     /locus_tag="R8G18_003116"
     CDS             complement(6548..8095)
                     /gene="fdrA"
                     /locus_tag="R8G18_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEE9796745.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(8085..8948)
                     /locus_tag="R8G18_003117"
     CDS             complement(8085..8948)
                     /locus_tag="R8G18_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEE9796746.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8988..9593)
                     /locus_tag="R8G18_003118"
     CDS             complement(8988..9593)
                     /locus_tag="R8G18_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEE9796747.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9851..10348
                     /locus_tag="R8G18_003119"
     CDS             9851..10348
                     /locus_tag="R8G18_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEE9796748.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10440..11372
                     /locus_tag="R8G18_003120"
     CDS             10440..11372
                     /locus_tag="R8G18_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9796749.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(11414..12502)
                     /locus_tag="R8G18_003121"
     CDS             complement(11414..12502)
                     /locus_tag="R8G18_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEE9796750.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(12644..>16268)
                     /gene="ehaA"
                     /locus_tag="R8G18_003122"
     CDS             complement(12644..>16268)
                     /gene="ehaA"
                     /locus_tag="R8G18_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HEE9796751.1"
                     /translation="GFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKGT
                     SRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQN
                     SDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEKK
                     ITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGITQ
                     NIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVASG
                     AVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFAY
                     TDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVDF
                     GALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNSA
                     IKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVNN
                     DGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVSL
                     SNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSADS
                     LLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLVS
                     HAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVLV
                     VKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIEL
                     IHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPNP
                     EPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3772 a         4391 c         3855 g         4250 t
ORIGIN      
        1 gctggtgctt ttttaagcga agtgtcgccg atcacaacaa aatatcggcg aaaaggcggt
       61 ggaatgccat ttggcatacg gatatacaga tgaaatgatt acctgctttg cgattcactc
      121 tctggtggaa taacaacttg caggtggaac cctttctgaa ctaaagagtg tagcggattt
      181 taacgtgctt atttacagga taatatctgt cacctaattc ctgcactcta ttagatatcc
      241 attcatttaa tcgtgcatca tctctgtatg tttttacgtc cagaacagag agcaataaca
      301 tcaccagtga ttcaggaaaa acagggtaga tattttttaa gtgtaataat aatccatgcg
      361 caagcgcctg cttaatgggc gcgtctgtct ccggaataga cgacatttcg cgaagccatt
      421 tttccacatg catacactct ttccctttag ggaaaagtct cagcaaaggc atatggtgtt
      481 caagaagaat acatttgcaa tctgttcgca ctgaattgat aaactgcact aatttcttaa
      541 gcccggtaaa gcgtccagcc tgcacatttg ctttaatatc aagaaacgca tcatcctggt
      601 catataccca aactgtccct ttttgatttt caagtatgcg aaaaacaaac ttaaccgggt
      661 gatcagggta atccatccag tcataaaatc ctgggaaatc attaaactgc acatttatgg
      721 tgagtgcgtt atcgtctgac cactcacagt ctcgtacggc agccatgaga tgaatgagtt
      781 tgtcgtcatc gactaaatca tcacgacaat cataaggata atcataatat tgtttaatca
      841 aaaccttgaa tatatcgaca caggaaacca gggagctttt gatatcctct ggtaactgat
      901 tgtttttaac cgtattaagt atgctttgtt gcgtatatgg catcaaatta ttgtgcggag
      961 cttttaatga tatcatcgcg atctctctta aataaaaata aacgtcgcag ataataaagc
     1021 gctgattaaa ccaggcacta tcagaaatcg ctcataacta acgtgagaag tattgtgtac
     1081 tggagggagt taatttaaca atttgaattt acgttatagg ttttactgct actttgatat
     1141 tatttcgctc tccttaacta aaaagttaag tggagagcga agttaatatg ttaaaccaca
     1201 gcgtatttat taattttttc aatctgttag tgtgcgatta tcgataacaa aacgatattt
     1261 cacatcacca cgcagcattc gctcataggc ttcattaatt tgatcggccc gaatcatctc
     1321 tatatcagcc acgatgccat gttcggcgca aaaatcgagc atctcctgag tttctggaat
     1381 gccgccaatc atagaatcgg ctatcgcacg gcgtttcatg atcaggttga aaacttccgg
     1441 cgatttatgc ggtgtcgcag gcgcaccaac cagcgtcatg gtgccatcac gcttcagcaa
     1501 ggtggtaaaa tcgtcgagat tatgtggcgc agctactgta ttcaaaatga aatcgaaact
     1561 cttcagatga gccgccatct catcggcatt gcgtgagtta acaacttcat cggcccccag
     1621 ggctttttcc gcttcgcgtt ttgcctcaga agtggtaaat gccaccacat gtgcccccat
     1681 cgcgtgggcc agcttaatcc ccatatgtcc cagaccgccg atgccgacca cgcccacttt
     1741 tttacccggc ccggcctgcc agtgacgtag cggcgagtac gtggtgatcc ctgcacacaa
     1801 caaaggagcc accgccgcca gctgctcttg cgggtgacga atacgcagaa catatcgctc
     1861 atgaacgacg atctgttgtg agtagccgcc cagagtatgg cccggttcgt ccggcgtcgg
     1921 cgagttatag gtgccgatca tgtgatcaca gtagttttcc agcccgtcct cacactcttc
     1981 gcaatgttta caactgtcga caatgcagcc gacaccgacc agatcgcccg gcgcatgttt
     2041 ttctacctga tcaccaacgg ctaccacacg ccccacaatt tcatgacccg gtacgcaggg
     2101 gtaaaccgtc cccgcccact cggaacggac ctggtggaga tcggaatggc agacgccaca
     2161 gtaagcgatt tcgattttga catcattcgg tcccggttca cgccgtgtga tatccatcgg
     2221 ttcaagtggt tgtttagcgg aatatgcacc aacagctttg atcttcattg tgtttactcc
     2281 tgattagcta tgtgtatttg gcgacgcctg atatgtcatc gcggcaaaac gcgtccattg
     2341 aatatagcca atatttaaac cacccgatcc agccccaaca tgccgcgaac ctgaatgaaa
     2401 cagagcagca acgttcccca taaggcgagc gcctataagt gtgatatttg ctatccgtaa
     2461 aagactaaaa tcggcaaaac tgatgatctt ttcacgtaga caaatagtta tctacacaac
     2521 gtcaataagg gcatattcct caaagataat gtccatttct gccgacacta tcatattgag
     2581 gtcgccccac tccctggggc ttaaatctct tacaattcac cgttgcaggc gtttcaaatg
     2641 cgcatcaacc tgccacactc ccccacacca gttgcccttt atggaacgtt gcggttctcg
     2701 gcgagatacg cgccaccgcc tcggcggaac aggaggcgtc caccagcaca aagctggcat
     2761 catcctgcgc ttttggccat acacgctcgc ctttttcatt cagcggcaat acatcgccag
     2821 tggctagaaa cagcgaacgg gagaggttct gttcgttagg acgaatatag agctgcgcgt
     2881 agagattggc tttttccagc atgtcgccca gaccataagg cgaccagtgg tcgataacgc
     2941 tgtcagtgcc agtcatcact tttacgcctt tgtcgtgcaa ctgtttgagc ggcatatgca
     3001 gcgtgccaat cggcaccgtc gaggcgatag aaatttgctg cgccgccatc cgattcgcca
     3061 gcgcatcgac ctgttgttcg ttgagcgtag ccagcgcaaa ggcgtggctg atggtcagct
     3121 tacctttcag ttgcggcgtt ttctctaccg tttcaaccat ataattgatg gctgccacgc
     3181 ccgacggagt ggtttcgtgc aggtgaatat cgacaccttt gtcgtagtcc agcgcaatct
     3241 gaaacatggt gtcgagggat ttttccatcg cgccatcgac actggtcggg tccagcccgc
     3301 cgacgtaatg cgcacccgcc tgcatcgctt cgcgcattaa gggttccgat ttcgacagca
     3361 gcaaaccgtg ctgcgggaag gcgacgattt cacactcgaa gcccgcctga cgccgcgcca
     3421 gcaccgcctg caaattttgc agatttttta ggccggaaac cggttcgata ttgcagtggc
     3481 tgcgggcaat ggtggtgcct ttcgactgca atagatcgat cagtttttcc gcccgctcct
     3541 gcgtatacgg ttgtaactcc ggcagcattt tctgcttgag tttgatcatg tcctggatag
     3601 tggtgcctgc cggacgattg agcgagcgcc acggcccgcc gtagaaggtt ttgtcgagat
     3661 gaatatgcat gtcgcgagtg gtgggtagca tcaacttacc gccagcgtca tagtgcggca
     3721 gcgtggcgtc cggatgctgc ttgttctcgc gcagggcgac aattttgccg tcctgaatct
     3781 ccacggtctg gcgcgccgtg cgggtctgca ccgccacacc gttttcgtag tcaaaaccgg
     3841 tttccagcag cacgttatcg agatagtaat gcgggtcagt gattttcatg gtgttgttcg
     3901 cttcgcagtt tagggtgcca gctaccgccg catgggcgag cggcacatag gtaccaaaca
     3961 gcgcggcggc ggtgaccatc ttaccgctct ggctcagaaa ttcacggcgg ctattgcttt
     4021 ctttcatcct acgtccttac gttttaataa tatgggtgcc cgtttcaccg cgcagcgccg
     4081 caggcaggca ttccggcgtg gtgataatca cttctttgcc gccctgctcc aggaacgtca
     4141 ggctggcgat gatttttggc aacatgctgc cgggagaaaa atgcccttcc tgcatatagc
     4201 gagtcatggt ggcaatatcc acccgatcca gcgcctgctg ctgcggttta ccaaagtgga
     4261 tacagacttt ttcgacgtcg gtggtgatca ccagaatgtc ggcgtgaatt tcacgggcca
     4321 gcagcgcggt agagagatct ttgtcaataa ccgcgtccac gctttggtaa tcccccgctt
     4381 cagtacgcac taccggaatt ccaccgccgc ccgcgccaat tacgacaaaa ccttgttgga
     4441 tcagcgcctt aatggcgggt gcttcgacaa tacgtttcgg ttccggcgag gcgaccacgc
     4501 gacgatagcc ccgtccggca tcttcaacaa aacaccagtc agggtttgcc ttttgtagtt
     4561 tgtcacgctg gctttcactg aagaatgcac caatgggctt ggtgggatgg gcaaaacctg
     4621 gatcgttttt atccacttcc acctgagtta ccacggtgac ggcttttttc tcgccgtgac
     4681 gcgccagtcg gttattcagc gcctgttgga tcagatagcc gatgccgccc tgcgtatctg
     4741 ccacacagtt cgccagcggc gttaagggca gcccttcgcg ctcgtgggca atctccgcac
     4801 ggcgtaaatc cagcccgacc tgcggcccgt taccgtgggt cagcacaatg tcgtaatcgg
     4861 aagccagcat ttccagcacc gtatcggcca cggctttcac cgcctccgcc tgatgctcaa
     4921 tcgactggct ggcgttatct ttgataatgc tgttgccacc aatggcaacg accacaagct
     4981 ctttcatgtt gttatgtccg ctacagaggg tgatgaggtt atgaccaacg ccagactgcc
     5041 tgatgcgcta cgcttatcag gcctacaatt ctcgggccat ccgtaggccg gataaggcgt
     5101 tcacgccgca tccggcataa acatgtaagt cagacgccgt aacgttcgca ccagccgaga
     5161 atggctttct cgaagcaagc gaatggtgga tgcacaatgc ccgcgccaat catgccgacg
     5221 cccgcatctt tatgggcgat ggcggtgttg atcaccggca gaatgccgct gctacccacg
     5281 cgggtgatgt cgattgccga cggcacgccc ataaaaccga gcagcggaat ggtgacgttg
     5341 gggttttcac cgagagtgat ttcacgcatc tgacgggaga aatcgatagc ctcttccacc
     5401 gtgccgccca ccagcgcgac gattgcaggc gcggtcgcca tagcaaatcc accaataccg
     5461 taggtttcgg tgatggcgct gtcgccgata tccagccccg aatcttccgg cttataaccg
     5521 gcaaacatcg ggccgatcac ctgctgcgcc gggccggtaa accattgccc cggcaggccg
     5581 ctgacccgca ggccgaactc gacgccgtta cgcgccatgg tggtgaccac ggtgctgtat
     5641 tcgatgccgt gcgccgcatc catcgccgct ttacacatcg ccatccacgt cgggccggag
     5701 aagtagtcgc tgctggcgac aaactcgaac acttcgcgct gttgctcgac ggaatagcct
     5761 gcctgaatga tccccggcgt cagtgcctga atcagcagcg tcgtcccggc gttattgcgg
     5821 ttatggcact catcgcccat atgcagcgcc tgcgccagca ttaagcgcag atcgatctca
     5881 ccgataattt tcatcgcgtc gcgcagcatt ggcccctgca catcgcgcat ccagttgagg
     5941 cggtcgatca cgctctgatc gttggcaccc atacgcaaaa tcttcgccat ctgctcgctc
     6001 atattggtgt aagcgatgtt gccgtaggtt ttgtttttca cgatgtgcat aaacatcgag
     6061 gccgaggtaa cgcccgccat cgatcccaca cagtcgtgtt cgtggcacgg cgagaaggtg
     6121 atctccccgg aggccgccag ttcagccgcc tcgtcgagat ctttcgccag tccttcgaac
     6181 accagcgctc cggtgaccgc gcctttcatc gcgccgcaca ttttttccca ggtgatcggc
     6241 ggcccggcgt gaagaatggt tttcggcgtc atgcccggca ccacgttaat cgcctgatca
     6301 aaaccaatca gcaccggatg cgactggata atgcgctcca gcgcctgctg gttagcggcg
     6361 gcgattttgt ctgccagcgg cgaatcggca atgttatcca gcgcctgcac cacctgcata
     6421 ttgccctgcc ccggcggcgt ccagtcgagt tgagtcactt ctacatgctg ctttttcagg
     6481 tcatcgctaa acatggcgat gccgacgtta ataacgttca atggttggct aaacagtgac
     6541 tggctcatca ggcttcctcc cctttgcaga taaattcacg cgccagcaat ccggtattgg
     6601 tgctgctgct cgccagaatc actccggcat caagcagcat ctggctttgt tgttctaacg
     6661 atggcgtatc aagatcggta cccagcacat aggcgagaat gatcaactcg cgtccttcgg
     6721 cagcggcgat cgctttcgct tctttgatcg cctcgatggt ggagccgacc ggatcttcat
     6781 gcgatccaaa tccgagcaca aaatccatca cgatcaccgc cacttctgga tcgcgcgcct
     6841 cttcgatcaa gcgactgatg cggttggtgg ggtcaatcat tggatgcggc ttgccgttcg
     6901 tgaagtcgtc atcgccaaag tcgaggaagg tgtgtttgat gctgcggttg atatcttgca
     6961 ggcggaattc cggatcgggc tgaatgttgc tgtagacatc gccatgtttt tccatcaccg
     7021 cgaacatggt ttcgtcgcac agcgtgccgc cgcagaacag gccacgaatg tatttctgct
     7081 gcggttgcag acgcgcacgc acatcagcaa tcaacggctg gttaagcgta tgcaggtcga
     7141 gattttcctg tttcacgccg gagagcatca ccgcttttag cgccgcctct ttgctgccgc
     7201 gggcaaactg tagcccctgc tcatccaccg gcgtttcaac gcgaccgagg aagcagacca
     7261 ccaccggctt gcggcaggca cgtgcgcgtt ccagcacttt gcgggccacc gcaggcgcgg
     7321 gaggttttga aatcagtgcg ataatttcag tttgcggatc gttttccagc atcccgatgg
     7381 cgtcgagcat catcaggccg ccgattttct cgctcaggtc gcgcccgccg gtgccaatca
     7441 gttgtgaaac accgccacca aattcatgaa tgcggacgct cagctcctgg ctgccggtgc
     7501 cggatgcgcc aacaataccg atattgccgc gacgcacggc gttaccaaaa cagagcgccg
     7561 cgccgttaat aatcgccgtg ccgcagtccg gccccatcat cagcagccct ttttcgtggg
     7621 ccaactgctt gagcgccagt tcatcttcaa ctgagacgtt atcggaaaac agcatcacgt
     7681 tgagatcgtt ttgcagcgcc tgacgcgctt cgcgagcggc aaacagaccg ttgaccgaaa
     7741 tcaccgccag gttactttcc gggatatgct ttttggcgct ggcaatagtg gcgtaacgcg
     7801 cctcgtgcga gccgctttgc gctttggtgt tgaacagttc ttcaatctcc accagcaact
     7861 gctcgttgtc cgcacccgat ttaccattga tgacaatcat caggtcgccg tttttcgcct
     7921 gttccagcgt cggcgtcagc agtcctaaat ttttcagcac acctttgttc atttcggtcg
     7981 ccatcgccac aaatgcctgc tcgacgccgt cgagtttatt tgcacgcgtg gagatagaca
     8041 tcagcgagac agaatcaaaa taggtattcg gtttaatgac tattttaact gacataattc
     8101 cctccgtagg tttggctcag cgcacaacca tcggccatgc catacagcat gtcgcagccg
     8161 gaactggagc caatatgttt aattttttct attgcctgag ttgaattccc agggacgcca
     8221 taaataatgt tgtgaataaa gcgatgaata ttttcccggc agcgttgttg taatgcggct
     8281 tccagcgtta tggcacttaa taacgtggtg ttatttctgc cgcgtagcag accgcgataa
     8341 aattcctctt tgtatttttc tgccggatgc ccgggaataa ataaaataag cgccagacct
     8401 gttaaataat catcggctga tggcgtaagg ccaatcccta acccaatcat actggcgacc
     8461 gctgcggcga tattttgctt atcgtttact gtctgaataa ctgcccgtcg tttaatatgt
     8521 aattctttat ttaacgcctg ataaaacgga ttatcgccgc gataattaaa cagggtttca
     8581 ttttccccca ggtgctgatg aataatgtcg tgccactgta gccaggcgat aaggtgaaaa
     8641 ttcacagcgg tcatttgcaa ggttggggac agccaacgcc gacaacgact catctctata
     8701 tgaagatgtt gaccaaccgt aataccttga tcgtcaaact gaaccttatc accatgccgg
     8761 aacagatcat caaagtgagt gagtgccaac cgacaactgt ttggcgcatt gtcgtactct
     8821 tcacaaagca gcgtcagcaa ctgctggcga gccgggagtg cgatatttac cgctctggca
     8881 aagacctgtt caacctgtcc ttgcccccgc tgcgccagaa aatccgcatc cgcagttagc
     8941 gcccacacac accgtttttc tggtgtgttg atagccagcc tcccggttta tgcacctgcg
     9001 gcaatcagta actgcgcaat ctcttcaaag ccccgttccc gcgccagttc cagtggcgtt
     9061 ttgccatatt tatcggtcag atgcgggctg gcaccgtgtt ccagcaataa ctgcacaatc
     9121 gcctgctgtt taataccacc atcattaagc acaatcgctt ccagtagcgg cgtccagcct
     9181 acatggttgg tctggttaac gttaatctcc gtatgcgcca aaagctcttt tacaatactt
     9241 aaatggcctt tttcacaggc aggcgtcagg ccgacaccgc caaaacgggt tacgcaatta
     9301 agatcgggtt tagccggtaa aataattcgt agtagcgtta aatcatcgtt cagacagcta
     9361 attaaaaaag gatttaaaca ggtatgatct tgcttattaa tatccgctcc ggcatcaatt
     9421 aatgcctgaa cgcaagcata ttgctgatat aaacttgcca gcgtaattgc cgttttcccc
     9481 tgacgatcgc aggtattaat atcgacaccc agcgcaaggc aggtttttac tttatcgata
     9541 tcaccctgtt gtgcagccaa taaatagtcg gcaggtagat tttttatact cattcacatt
     9601 cccctggttt ttgtggctgt tttcgatgaa ttgagaatag caacgataat ctgataaaag
     9661 aacccgctta aatttaattc gccgtatctc gcctttttgt tgaataatat tcaacaatct
     9721 tttcgcagtg tgcggtagtg caatatttgt ttctttgacc cgataccctt tcactcaatc
     9781 aggttttatt tctctatagt ctgcacatgt aagagtagct ttcgtccatg tctaaccaga
     9841 ggggaacaag atgaacggac tcacggcaac gggtgtcacc gtcggtattt gtgcagggct
     9901 ttggcagtta gtgtcttcac atgtcgggct ctctcaagga tgggaattat taggaaccat
     9961 tggcttcgtc gccttttgca gtttttatgc cgcaggcggc ggtaaatcgg ggtttatcag
    10021 aagtctggcg gttaattatt ccgggatggt gtgggcgttt ttcgccgcgc tgactgctgg
    10081 ttggctggcc tctgtaagtg gactgtccgc gttctgggcg agcgtaatta ctaccgtacc
    10141 cttctcggcg gtagtggtct ggcaagggcg tttctggtta ctgtccttta ttcccggtgg
    10201 ctttctcggc atgacgctgt tttttgccag cgggatgaac tggacggtga cgttactggg
    10261 ttttctggcg ggtaactgcg tggggataat ttcagaatat ggtggacaaa aattaagcga
    10321 agcgacgact aaacgcgacg ggtattaaaa gttattcaaa aatgattatc tatctttaac
    10381 cgatacctct acagtcatag ctggagaaat tcatagtaga taatggcgag aatggtttaa
    10441 tgaactcaat ttttaccgaa gagaatctgt tggcatttac taccgctgcg cgttttggca
    10501 gcttcagtaa agccgcagaa gagttgggtt taaccacttc cgccattagc tacaccatca
    10561 agcgtatgga gacggggctg gatgtggtgc tgttcactcg cagtacccgc agcattgagt
    10621 taacggagtc cgggcgttat tttttccgca aagctaccga cctgctgaat gatttttatg
    10681 ccatcaaacg cagtatcgat actatttcgc agggcataga agcgcgtgtg cgcatttgta
    10741 ttaatcagct gctttatacg cccaaacaca ccgcacgctt gttacaggtg ctgaaaaagc
    10801 agtttcctac ctgccagatt accgtcacca ccgaagtgta taacggcgtc tgggatgcga
    10861 ttattaataa tcaggccaat atcgccatcg gcgcacccga tacactactg gacggcggcg
    10921 gtattgatta cactgaaatt ggcgcgattc gctgggcttt tgctatcgcc ccggatcacc
    10981 cgttagcctt tgtgccggaa cccatcgccg aaagccaact gcgtctctac cctaatatta
    11041 tggtggagga caccgcgcat acgattaata aaaaagtggg ctggttgctg cacgggcagg
    11101 agtcaattct ggtgccagat ttcaacacca aatgtcagtg ccagatttta ggtgaaggaa
    11161 ttggtttttt gccggattac atggtccgtg aggcgatgac gcaatccctg ctggttaccc
    11221 gacaaatcca taatccgcgc caggattcgc gcatgttact ggcgactcag cattctgcta
    11281 cgggtcaggt gacgcagtgg attaaaaaac aatttgcgcc aaatggcata ttaacgggga
    11341 tctatcagga tttactgcat cgggagaatt aatcgctgcc aacctcccga tgcgctgtga
    11401 atagcggttt acatcaacca cctgctttca ttacccattc agagataaat ttattacccg
    11461 gtactggcgg agagtacaaa tagccctgca aaaagtgaac gcctttaccg atcattaaat
    11521 ccgcctgctc ctgggtttct accccttccg ccacgatact cagaccaggc ttacgcgcta
    11581 gttcgacaat attgtccaca atatgaccgg agatttcgtc gacactcgcc atttgcacaa
    11641 atgacttatc gatcttaata aaatcgaccg ggaacgcctg caagtaacga taggtcgcat
    11701 aacccgtacc aaagtcatcc agcgcaaagg taatgttgtg ctgatgaagg ctgtcaaata
    11761 tcgctctggc ttctggcgtt accggaatag ggttacgttc cgttagctcg agaaccagct
    11821 tgattttatc gttacctaat ttcttaacca ggttcagaca ctctttttca aatcccgctg
    11881 ccaaaaaaca acccgccgag acgttgatgc caatatggaa attgtccggc agcaaatgtt
    11941 ttaccggcat cagaatatcc gcagtctgtt tcatcagttg gcgggtcatt atgacaataa
    12001 gaccggatga ctccgccaga ggaataaact gatccggtgg gataattccc gtttgtggat
    12061 gttcccagcg gacaagtacc tcacagcccg tcagtacccc agtctgcgcg cagaaaaccg
    12121 gttggatcca cggtttgaat tcgtggtttt ccaacgccag actgatggct tcaggcgtga
    12181 cgatatggtg acaacggtta tcagggatat aaaaattatt tttatttccc gtgccactga
    12241 tgacttgcgg atgatattga aaaaagatat cccgccagaa ggtaatatca ctctggatgc
    12301 ctagtttttc atacagctgc ttcttttgat gtgaaattgt ctttgcactg cgatttctgt
    12361 actgtgagat ttctgtaacc gacattccct ccattagcag cttgagtaaa agccgttctt
    12421 tttcggacac gataccaggc aacgtcaaat tcaccgttgc cccgaactct tccaaaaaaa
    12481 cactcaaatc atgcgaagcc attgaacaca ccttgattaa atcttgtgcg ccagctaaat
    12541 ctgcgagtct tcactctgac tgataaagct ggctgcaaga aaatgacaca gagagccgaa
    12601 ttatctcccg ttcggctctc tctcctttta ctcattattc agatcagaat tgccacttaa
    12661 tgccaaccat tgcagaggtg tcattatatc ccctatcggc aacctgaacg cccacattcc
    12721 cccagacatt caggttggca tttagctgtc cttccacccc ggtttttatc tcagcaatat
    12781 ttcgggctcc atcctgagtg acggacacgc catccatact ggtgctgaaa tccttactgt
    12841 tatgcagcca gttcacttct acaaacggct ggaactcgcg ggatttaccg tcatccattt
    12901 tatggtggct cttcagccag gtttttacgc caagtcgggt ttgaacattg ccatcaccgt
    12961 tgctatgaac gagggttccg ttgctttcgc ggtgcttatc ggctttgact cccatccagg
    13021 taacctgtgc ttgcggctga acataccatt cattacgcgt tccctggctg ccattaaatt
    13081 cagctaattt gtgtttgtat ccagcttcca gtgaagcggt aaatcctttt gatttatagg
    13141 attcactttg taagtcatcc cctttcactg tgttatcaaa ccagctgtac tgcgcccaac
    13201 tgtcgagata cgcgccatta cgcgactcgt catcggcata ccaggtggca tagaggccgg
    13261 tgctatatcc gttcacactc gcttttgcac gataaccggt tcgcgaggaa atggttttgc
    13321 tgtcgctgtt gccatatccc gccatgaccc caacatgcca gctgtcgctg ccgttttggc
    13381 tccactgcgc gacatcgcct cccagttgca gaacatagcg attgctttgg gttttcagct
    13441 ggccgctgcc atcacgccat ttattatgac caccttcatg gcgcatccac atagtggttt
    13501 gtttctgctc acccgtcacc atgtcggtat agtacgtatt acccagacgc tcatgcagac
    13561 gcgtggtgaa cattgtattc gctgctgcaa ggttcgcaat gtagctaccc gcctccggtc
    13621 gcaggtcatt atccacattc agatccggac ccggcgtagg tgtcgggtta gggttcggct
    13681 ctggattcgg gtttggctcc ggattcggca tcgggtctgg ctccggctgc agttcaggag
    13741 aatcactgcc gctggtcaga taccagttac cactatttgc cccttgtcca cgcgcgagag
    13801 tgtagtcata cgcccccgca acgatacgac cagcctgaac aaattcgccc tcagacttac
    13861 cgtctacatg gataagttcg ataccgttaa gggttttcgc acctgtaccg ccagcgttat
    13921 tcaccgtaac ggcagtcgtg ccgctagtat cgccctcgac aaccaattta tcggttactg
    13981 agtcatcgcc attcagtacc gtattcatta ctagttgacc gttattaccg tggtagttcc
    14041 ccttcaccac taacacattg ccagcttgct gtcctgacat tccggtcatc agcgtaccgt
    14101 tgttggtcag attgccgccc agggtgtaat tcacagacgc agccgctctt ttttcagcgc
    14161 tattattcaa cgccagggta ccgttgttct caacgtttgc gctacccaat ccctgcgcat
    14221 gacttaccag gacattagcc gaattagcaa tgttcacatt cgctgtcagg ttgctgttat
    14281 cgcccgcgat gtccagcgtg ccgccctgaa tattcagctc tccgctcccc gttaacgaac
    14341 cggttgaagt gcccccgttg gtaaccgtca gactgccgcc atttaagctc agcagtgaat
    14401 cggcgctgcc attgagcgtg cccacggtct ggctgtgacc attcatatcc agttcagtct
    14461 ctgccgccag acgcagttca tgggtattac ccaacacgtt atcgttattc aacaaaagcg
    14521 tcccactgcg cagatcggta acaccggtat agtcgttgtc tttgttagaa agcgatacgg
    14581 tctgaccctt ctggctgctg aatgccaggt caccactgcc ggtaatcttt gcgctgagat
    14641 cggcggcatt ctcgctttta ccgttagagc tcagcaccag agcatcgctg tcggtagcgt
    14701 gtaaatcaag ctgggtcagg ccgtaaccga tatacagacc gtcattgttt accccactcg
    14761 tcagacgata gtcgtaagtg ccgacggccg ctttttgccc attctgctga atatcacgtt
    14821 gggcactgct ggagaggatt tgcccatttt tatcctgcaa ttgcagattg cccgcatcgc
    14881 ccagaacttc cgcaccttgc gcatcaacca gcttaatggc gctgttacca tcatcgactt
    14941 cagtgagcga taacgcagag tcaatatcac gagatactga gcggacaacg tcactgtcag
    15001 aaacctgaat cacaccctcg ccgcgcaaat ccagcgtttt actaacgttg accgtccctt
    15061 ctgtcatctg tgcacctgct gtcagggcac caaaatcaac ggttccgccg gagaatccca
    15121 aagtgctaat ggagtgagcg cccttaccca ctgttaccag gcttccctga cccgcagtga
    15181 tcccgcctct ctgcagaaac tcagcattct tttccaggtt aaatgtcgtg ttctggaacg
    15241 ccagtgaacc ctggaaccag tctgtataag caaactcatc gaaggagaat tcgtgattcc
    15301 ccatgtttat ttcaacaata cctctacctt gaagatcgtt atcaaatacc caatctttat
    15361 tggcattgat ttgcagtttg ccatcaacat ccaggtccgc acggccgatg ttcttgtctt
    15421 cagaaataac cgcgacagca ccagaagcga catcaatata tccgctgaat gcatagttat
    15481 tgccggtcag cgccactgtc ccactgtcga tacttattgt accgttgccg gaaatcttat
    15541 tggcaaaggt catgctatcg tcagccttca ggtgtaattt cccgcggttg ttgatttctg
    15601 cggtacctat attttgggtg atccctaaag cgccattttg ctcaattttg actttaccgc
    15661 taaaggcgga gttatccccg gttaagtaag tcgtaccatt gagcgcattg atatagccca
    15721 ggccctcgct gtcactgaca atatccgcgg aaatagtcgc gtttttatcc gtgtggttta
    15781 aggtgatttt cttctcggat gtctttgccc acacaaactc aattttttcg gcatcaataa
    15841 tccctgcagc cttcgctgcg cttccttcct gtgcacctaa cgctaactca gagttggtgc
    15901 ttaaactaat agtcggcgca gagattttac caccttccga aacagcaagt gttgtatcgg
    15961 agttttgatc ataataaccc aggctaattt tctcactggc attaacttca ccgccgttat
    16021 taactgacag gtactgatta ccaccgtaac tgccgtaaac gcccataaag aaggttttag
    16081 aatttacctg gctgccttca ccactgacat ttccctgcaa ataggtcaga cggctggtgc
    16141 ccttaacatg gattttgccg ccgttggaga cattaaaata gccgcgatcg tgtctcatgc
    16201 ctgagtttga ctcatacaaa tcatcagcat ataactcgct gccagcattg gagacattca
    16261 ggaacccc
//
LOCUS       DAQSWQ010000092        16250 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.093, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000092 DAQSWQ010000000
VERSION     DAQSWQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.093"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..578
                     /locus_tag="R8G18_003123"
     CDS             <1..578
                     /locus_tag="R8G18_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HEE9796752.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="R8G18_003124"
     CDS             581..1744
                     /locus_tag="R8G18_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEE9796753.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="R8G18_003125"
     CDS             1828..3453
                     /locus_tag="R8G18_003125"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEE9796754.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="R8G18_003126"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="R8G18_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HEE9796755.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="R8G18_003127"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="R8G18_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HEE9796756.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="R8G18_003128"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="R8G18_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEE9796757.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARWLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="R8G18_003129"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="R8G18_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HEE9796758.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="R8G18_003130"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="R8G18_003130"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEE9796759.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="R8G18_003131"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="R8G18_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="R8G18_003132"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="R8G18_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HEE9796760.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9723
                     /gene="ulaC"
                     /locus_tag="R8G18_003133"
     CDS             9262..9723
                     /gene="ulaC"
                     /locus_tag="R8G18_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HEE9796761.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9737..10387
                     /gene="ulaD"
                     /locus_tag="R8G18_003134"
     CDS             9737..10387
                     /gene="ulaD"
                     /locus_tag="R8G18_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEE9796762.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10397..11251
                     /gene="ulaE"
                     /locus_tag="R8G18_003135"
     CDS             10397..11251
                     /gene="ulaE"
                     /locus_tag="R8G18_003135"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HEE9796763.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11251..11937
                     /gene="ulaF"
                     /locus_tag="R8G18_003136"
     CDS             11251..11937
                     /gene="ulaF"
                     /locus_tag="R8G18_003136"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HEE9796764.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12034..12585
                     /locus_tag="R8G18_003137"
     CDS             12034..12585
                     /locus_tag="R8G18_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HEE9796765.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12660..12935)
                     /locus_tag="R8G18_003138"
     CDS             complement(12660..12935)
                     /locus_tag="R8G18_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEE9796766.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13262..13657
                     /gene="rpsF"
                     /locus_tag="R8G18_003139"
     CDS             13262..13657
                     /gene="rpsF"
                     /locus_tag="R8G18_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEE9796767.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13664..13978
                     /gene="priB"
                     /locus_tag="R8G18_003140"
     CDS             13664..13978
                     /gene="priB"
                     /locus_tag="R8G18_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEE9796768.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13983..14210
                     /gene="rpsR"
                     /locus_tag="R8G18_003141"
     CDS             13983..14210
                     /gene="rpsR"
                     /locus_tag="R8G18_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEE9796769.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14252..14701
                     /gene="rplI"
                     /locus_tag="R8G18_003142"
     CDS             14252..14701
                     /gene="rplI"
                     /locus_tag="R8G18_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEE9796770.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14813..15784)
                     /locus_tag="R8G18_003143"
     CDS             complement(14813..15784)
                     /locus_tag="R8G18_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEE9796771.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15837..>16250)
                     /locus_tag="R8G18_003144"
     CDS             complement(15837..>16250)
                     /locus_tag="R8G18_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEE9796772.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3818 a         3967 c         4321 g         4144 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tgctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggt ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtca cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt tgaacagcac
     9421 ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc ggaagagggc
     9481 gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt caaccacgaa
     9541 gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa cacgcaccag
     9601 gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt cgaccgttta
     9661 cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa cgcggcagct
     9721 taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca accagactat
     9781 ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta tcgaagtggg
     9841 taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag cgctctaccc
     9901 gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc tttctcgtat
     9961 gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata tcaacaccgc
    10021 caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg aactgaccgg
    10081 ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc aggtggttta
    10141 tcaccgcagc cgtgacgcgc aggccgctgg cgtggcgtgg ggtgaagccg atatcactgc
    10201 gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggcc tggcgctgga
    10261 agatctgccg ctgttcaaag gcattccaat tcatgtgttt atcgccggtc gcagcatccg
    10321 tgatgccgct tctccggttg aagccgcgcg tcagttcaaa cgttccatcg ccgaactgtg
    10381 gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg aaaaagcgct
    10441 ccccgccggg gagtgctggc tggaacgcct gcgactggca aaaacgttgg gcttcgattt
    10501 tgtcgagatg tcggtagatg aaactgacga gcgcctgtcg cgcctcgact ggagccgcga
    10561 gcagcgtctg gcgctggtga atgcgattgt tgaaaccggc gtgcgtgtgc cgtccatgtg
    10621 cctttctgcc catcgtcgtt tcccgttggg cagcgaagat gacgcggtgc gggcgcaggg
    10681 gctggagatt atgcgtaaag ccatccagtt cgcccaggac gttggcattc gcgtgatcca
    10741 gctggcgggc tatgacgttt actatcagga agccaataac gaaacgcgtc gtcgtttccg
    10801 tgacggcctg aaagagagcg ttgagatggc gagccgcgcg caggtgacgc tggcgatgga
    10861 gatcatggat tatccgttga tgaactccat cagcaaggcg ctgggttacg cgcactatct
    10921 caacaacccg tggttccagc tctacccgga tatcggcaac ctgtcggcgt gggacaacga
    10981 tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc gcggtgcatg tgaaagacac
    11041 caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt gtggtggatt tcgaacgttg
    11101 cttcgaaacg ctcaaacaga gtggctattg cgggccgtac ctgattgaga tgtggagcga
    11161 aacggcggaa gacccggcgg cagaagtggt gaaagcgcgt gattgggtga aagcgcgcat
    11221 ggccaaagca ggcacggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa
    11281 gccaacatgg atctgccgcg ctatgggctg gtgaccttta cctggggcaa cgtcagcgct
    11341 atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg
    11401 aaagcagacg atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc
    11461 ccgtcttccg acaccgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc
    11521 attgtccata cccactccac tcatgctact gcatgggcgc aggcggggct ggcgatcccg
    11581 gcgttaggca ctacgcacgc cgactacttc tttggcgaca tcccgtgtac gcgcggatta
    11641 agcgaagaag aggtgcaggg cgagtatgaa ctgaacaccg gcaaagtgat tatcgaaacg
    11701 ctgggtgacg ccgagccgct gcatacgccg gggattgtgg tgtatcagca cggtccgttc
    11761 gcctggggga aagatgccca cgacgcggtg cataacgcgg tggtgatgga agaagtggcg
    11821 aaaatggcgt ggattgcgcg cagtattaac ccacaactga atcacatcga cagcttcctc
    11881 atgaataaac acttcatgcg taagcacggt cctaacgcct attacgggca gaagtaagca
    11941 gtgcgccccg gaattattac ctcctgcggg atgtaattgg tctgattccg gggccgcttc
    12001 agttaaaaaa tcgtataaac aacaggtgtt tttatggcta tcaatggatt gtttgtcaga
    12061 acggatccgc aacgtcgccg ctatggcgtt gcgttacttg tgggtgtact ttcaggaatt
    12121 atttcagcat ttgttaaatg gggagcggaa gtccccttgc caccgcgcac attttccggt
    12181 ggtcgcgatg aatttaaccc gccgtatatc tttctacggg attatttagg tatcgaccca
    12241 acccaaactg tgtatacatt ctcggaacat attatcaacc cggtgatggt gacgcatatc
    12301 atcttctcac tggtttttgc gatagggtat tgtattgtcg cggaaatttt cccgaaagtg
    12361 aaattatggc aggggatatt agcagggctt attgtgacag tggcagtcca cggtatattt
    12421 tgcccggcgc tgaatttaac gccgccgctc actcaattgc cttttgacga atatgcctct
    12481 gaaatcctgg ggcatatttt ctggttctgg ataatagaaa ttatgcgccg cgacttacgt
    12541 aatcgcatta ctcatgagcc agatccggaa gtcgcaatcc ggtgaataac cgcataaaaa
    12601 agccccctgc gaagggggca aagcaaacta tggcaatgtt tcgttggtta tacctggtgc
    12661 tagcgataaa tatccgcgct ggcgtgcata ttgccgttac tccccggttc ccgcatcaga
    12721 attacgtggt agtacgttgc gccctgcgca tctgtctctt catttaatgc ctgacgcgct
    12781 tcgctttcag tggcgaaatt atgattgata taaatcacgc ctaagctttg cacatcatcc
    12841 atgtttctgg cctggtggtt attaatttca atggctgccc atgtatttgc acttagcaaa
    12901 agcacagcca gaagggctaa aactcgactg aacatagata cctcctcgac ggctgacttt
    12961 gtgtgctctc ctctgtgatg atcttctgat ttaattttaa tcaatgataa agaagttgat
    13021 ggtgaccatt tctgatgcag ttgttcaaaa aaacaccatg atgaagtgtg atgaactcca
    13081 aatcagcggt ttagaggtta attgcgaaag gggagattta tttcggctct gcccttgagt
    13141 ttagcggggc atacaagtac tataacgcgt catttttcag ccgaccttta acacgttcct
    13201 tgcctccccg ggattcggct gacccagaca ggaggctgaa taatccgtaa ggagcaattc
    13261 gatgcgtcat tacgaaatcg tttttatggt ccatcctgac cagagcgaac aggttccggg
    13321 catgatcgag cgctacactg ctgccatcac tggtgcagaa ggcaagatcc accgtctgga
    13381 agactggggc cgccgtcagc tggcttaccc gatcaacaaa ctgcacaaag cacactacgt
    13441 tctgatgaac gttgaagctc cgcaggaagt gatcgatgag ctggaaacta ccttccgctt
    13501 caacgatgcc gttatccgca gcatggttat gcgtaccaag cacgctgtta ccgaagcatc
    13561 tccgatggtt aaagcgaaag acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac
    13621 cgctgatgat gctgaagctg gggattctga agagtaattt ctgatgacca accgtctggt
    13681 gttgtccggc accgtgtgca ggactcccct tcgaaaggtc agtccatcag gaattcctca
    13741 ctgccagttc gtgcttgagc atcgttctgt gcaggaggaa gccggttttc accggcaggc
    13801 gtggtgccaa atgcccgtta ttgttagcgg acacgaaaac caggccatta ctcacagtat
    13861 aacggtcggc agtcgcataa ccgttcaggg gttcatttca tgccacaagg caaagaacgg
    13921 actgagcaaa atggttttgc atgccgagca gattgaattg atagattctg gagactagcc
    13981 atatggcacg ttatttccgt cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag
    14041 agatcgacta taaagatatc gctacgctga aaaactacat caccgaaagc ggtaagattg
    14101 tcccaagccg tatcaccggt acccgtgcaa aataccagcg tcagctggct cgcgctatca
    14161 aacgcgctcg ctacctgtcc ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg
    14221 tccattaata cgactttgag aggataaggt aatgcaagtt attctgcttg ataaagtagc
    14281 aaacctgggt agcctgggtg atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct
    14341 ggtaccgcag ggtaaagctg ttccagctac caagaaaaac attgaattct tcgaagcacg
    14401 tcgcgctgaa ctggaagcta aactggctga agttctggca gctgctaatg ctcgcgctga
    14461 gaaaatcaat gcactggaaa ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact
    14521 gttcggttcc atcggtactc gcgacatcgc tgacgctgta actgcagctg gcgttgaagt
    14581 ggctaagagc gaagttcgtc tgccgaacgg cgttctgcgt accactggcg agcacgaagt
    14641 gagcttccag gttcacagcg aagtattcgc gaaagtgatc gtaaacgtag tagctgaata
    14701 attcgttatt caacgagacg taaaaacgcc gaccagtggt cggcgttttg cttttgtagc
    14761 gagcacttgc agatatcgat aagggccaca ataagcagcc ctacgaagat aattacttag
    14821 ctgcctgaat atcagccagc attggggcaa gggctgggtt gtccttagcg aagtttgcgt
    14881 acattggctg tactttctcg ataaacggag ctttatctac ctggtagact ttcacgccca
    14941 ttttatccag ttttgcaaat tcttgtttct cttcctcgct ccacaagtct ttcatgtagc
    15001 tcattgattc tttagctgct tttaatataa ctgcctgatt ttccgggcta agtttatcaa
    15061 acgcagcatt gctcatcaga accacatcgg gaaccatagt gtgttcgtca aggctgaagg
    15121 attttgccac ttcaccgtga cgcatggtgg tcagcgccat cacgctgttt tctgccatat
    15181 cgaccacacc ctgctgtaag gctgtataca actcgccgta atccagtggc gtcgggttac
    15241 cgcccatgac tttgatcatt tcaactgcgc cagggcttgg ctggacacgg actttcatgc
    15301 ctttcagatc gtctggtttc agtactggtt tattgccata gaaactgcgg gcgcctccat
    15361 cataaaaagt cagcccgaaa taacctttgc ttttcgttga gtcgaggatt tttctcccga
    15421 tatcgccctg catgacctga taataatgat cacgatcgcg gaacaaataa ggcaggctaa
    15481 ataatttata ggaattttca aatgcttcca tttctgccgc attggttttt accagtggaa
    15541 tagcgccaga acgaatcagc tccattgttt ctcgttgagt acccaatgtt ccatttggat
    15601 aaatacgaat agtaatatca ccgttagagt actctttgtt ctttttcgca aagaactcca
    15661 tcgctttatg aacaggatga gacttatcct gattatgact cagttttaat gtcactgccc
    15721 aggactggct tgcggataac ataagaacag caccgataat gccagcggat acttttattt
    15781 tcatagatgt tccttttcta ataaatatgg cggcaggtgc cgccagtgaa tgcgcatcag
    15841 tagcccagtg cttttggcaa tgccatactg atttgcggaa tataggtcac cattgccagc
    15901 accaatacca tcacggcata gaatggcagc ataggtttga taattttatt aatcggtaat
    15961 ttagatacac tacaaccgac aaataacaca ctgcctacag gcggagtaca aataccgata
    16021 cacatgttca gtaccatgac aataccaaag tgaatcgggt ccatgcccag ttcggtgaca
    16081 acaggtaaaa atatcggcgt aaaaatcagt accgccggtg tcatatccat aaaagtgccg
    16141 ataatcaaca ggataatatt gataataagc aggattatta ttgggttgtc agaaatagtg
    16201 ttaagcaaat cgagaatcag gaagggaacg tcagcgtttg acattgccca
//
LOCUS       DAQSWQ010000093        16167 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.094, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000093 DAQSWQ010000000
VERSION     DAQSWQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16167
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.094"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..173
                     /locus_tag="R8G18_003145"
     CDS             <1..173
                     /locus_tag="R8G18_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEE9796773.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="R8G18_003146"
     CDS             complement(345..674)
                     /locus_tag="R8G18_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEE9796774.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="R8G18_003147"
     CDS             complement(679..864)
                     /locus_tag="R8G18_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEE9796775.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="R8G18_003148"
     CDS             complement(861..3500)
                     /locus_tag="R8G18_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HEE9796776.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="R8G18_003149"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="R8G18_003149"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEE9796777.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="R8G18_003150"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="R8G18_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HEE9796778.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="R8G18_003151"
     CDS             complement(5227..5493)
                     /locus_tag="R8G18_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEE9796779.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="R8G18_003152"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="R8G18_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEE9796780.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9657..10790
                     /gene="ompN"
                     /locus_tag="R8G18_003153"
     CDS             9657..10790
                     /gene="ompN"
                     /locus_tag="R8G18_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HEE9796781.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10931..11365
                     /gene="uspF"
                     /locus_tag="R8G18_003154"
     CDS             10931..11365
                     /gene="uspF"
                     /locus_tag="R8G18_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HEE9796782.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11951..12865
                     /gene="sitA"
                     /locus_tag="R8G18_003155"
     CDS             11951..12865
                     /gene="sitA"
                     /locus_tag="R8G18_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HEE9796783.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12865..13692
                     /gene="sitB"
                     /locus_tag="R8G18_003156"
     CDS             12865..13692
                     /gene="sitB"
                     /locus_tag="R8G18_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HEE9796784.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13689..14546
                     /gene="sitC"
                     /locus_tag="R8G18_003157"
     CDS             13689..14546
                     /gene="sitC"
                     /locus_tag="R8G18_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HEE9796785.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14543..15400
                     /gene="sitD"
                     /locus_tag="R8G18_003158"
     CDS             14543..15400
                     /gene="sitD"
                     /locus_tag="R8G18_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HEE9796786.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15601..>16167)
                     /locus_tag="R8G18_003159"
     CDS             complement(15601..>16167)
                     /locus_tag="R8G18_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HEE9796787.1"
                     /translation="TVEIKKIINPRYTESGAVDCDVFFDDRDQAVPYTATADDVAPTG
                     QRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQGIEAWRTEQEAKPFTFEWNGRIWN
                     AGPNSLGRLYPVVMAAKSDIVRDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERND
                     EIYRRQRELKEELNSLKDLNSVRNFIVE"
BASE COUNT         4028 a         4090 c         3888 g         4161 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9481 tattatcttc gatacattca ccagacttat tatcatttca gaagaattat ttgattaagg
     9541 ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt
     9601 tttcactccc cggaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga
     9661 aaagcaaagt actggcactt ttaattcctg ccctgctcgc cgcaggtgct gcacatgcag
     9721 ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc
     9781 attatttttc tgataattca gcgaaagatg gcgatcagag ctatgcgcgt ctgggtttta
     9841 aaggcgaaac ccaaattaac gatcaactca ctggctacgg tcaatgggaa tacaatattc
     9901 aggcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccgggc
     9961 tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca
    10021 tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga ctcttatacc aatgcagaca
    10081 actttatgac tggtcgtgcc aacggcgtcg cgacttatcg taatactgat ttcttcggtc
    10141 tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaaggt gccagtaatg
    10201 gtcaggaagg caccaacaac ggacgtgatg ttcgccatga aaatggtgac ggctggggtc
    10261 tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcatctg
    10321 accgcaccaa tgaccaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga
    10381 ctgctgggct aaaatacgat gctaacaata tttacctggc aaccatgtat tcagaaacgc
    10441 gtaatatgac cccgtttggc gacagcgatt atgctgttgc aaacaaaacc cagaactttg
    10501 aagtcactgc acagtaccag tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt
    10561 ctaaaggccg tgacctgcac gctgccgatg gtgcagacaa cccggcaggt gttgatgata
    10621 aagatcttgt taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca
    10681 cttatgttga ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaacg
    10741 gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc
    10801 ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt
    10861 gaactccgtc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac
    10921 aggaggtttt atgaacagaa cgattcttgt ccctatcgat atttccgatt cagaattaac
    10981 tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gacgatgcag aggttcattt
    11041 cctgacggta ataccttcac tgccttacta tgcctctctg ggtttagcgt attccgcaga
    11101 attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa
    11161 aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga
    11221 ccgcattctg gaattggcga agaagatccc cgctcatatg atcatcattg cttcccatcg
    11281 accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga
    11341 gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat
    11401 tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg ccacagtttt
    11461 atatcagatt tcttatgctg ggcgttccgg catgacgcat actcttctga tgccatataa
    11521 cgaattgagt cgcttttaaa tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa
    11581 cctgaacaag taagggcaaa aatcacaact atctgaccca taagtatttt atttattttt
    11641 caatatgtta aacattatca gcgacaacaa taagctacga taacccactc tttttttgcc
    11701 ccttttttgc cccttctaca gcgttttgcc ccatttttgc catcaacaat tttcctcata
    11761 aagtcatttc aaagcctaat agctgctcta cttttttcct caatatcatg tcgccagcag
    11821 gaaacaacct aatccctgtt ttaggcctca aagctgttaa tgataatcat tttcattttg
    11881 tatgggttat gctataaaac atagccgttg ctatgtttgt tctaattttg ctcactatag
    11941 gtactaaatt atgcactcaa taaaaaaagt aaccatgctc ttggggtgcc tcgccctcac
    12001 ctgctcgatc gcatttcagg caagtgcaac tgaaaaattc aaggtcatta caacatttac
    12061 catcatcgcg gatatggcca aaaacgtggc tggagatgct gcagaagtct catccataac
    12121 caagcctggt gcagaaattc atgagtatca gcctacccct ggcgatatta aacgtgcgca
    12181 gggggcacaa ctgattctcg ccaatggtat gaatctggaa ttgtggttcc aacgctttta
    12241 ccagcatctc aatggggttc cagaagtaat tgtctcttcg ggtgtgacgc cagtagggat
    12301 caccgaagga ccctatgagg gcaaacctaa tccccatgca tggatgtcgc cagataatgc
    12361 tctgatttac gtcgataata ttcgtgatgc gttgataaaa tacgatccgg caaatgcaca
    12421 aacctaccaa cgtaatgccg atacttataa agccaagatt acccaaaccc ttgcccccct
    12481 gcgtaagcag attacggaac tccctgagaa tcagcgatgg atggtcacca gtgaaggggc
    12541 tttttcttat ctcgcacgcg atttggggct aaaagagctt tatctgtggc cgattaatgc
    12601 cgatcaacaa ggaacaccgc agcaggtacg taaggttgtt gatatagtta agaaaaatca
    12661 tatcccggca gtctttagcg agagtacgat ttccgataaa ccagcgcgtc aggttgcgcg
    12721 tgaaaccggc gcacactacg gcggtgtgct ctatgtcgat tccctgagca cagaaaacgg
    12781 cccggtacct acgtatatcg accttcttaa ggttactacc agtacgctgg tacagggaat
    12841 taaagccggg aaaagggaga aataatgatg caatctgcag gcattgtcgt taatgatgtt
    12901 actgtcacct ggcgtaacgg gcacacagca ctgcgcgatg catccttcac agtaccaagt
    12961 ggatctattg ccgctctggt tggggtaaac ggttccggaa aatcgacgtt gtttaaagcg
    13021 attatgggat ttgtgcgtct gaccagcgga aagatatctg ttctgggtat tcccacgcga
    13081 caggcgctac agaaaaacct ggttgcctac gtccctcagt cagaagaggt tgactggtca
    13141 tttcctgtac tggtggaaga tgtggtgatg atgggccgct atgggcatat gggtatgttg
    13201 cgtatagcca aaaagaggga tcgtcagatt gtcaccgatg cgctggaacg tgtcgatatg
    13261 gtggattttc gccatcgtca aatcggcgag ctttccggtg gacaaaagaa acgcgtcttt
    13321 ctggcgagag cgattgcaca gcagggtgat gtgatcctgc tggatgaacc ttttaccggc
    13381 gttgatgtga aaaccgaagc gaaaatcatc agcctgttga gggagttacg cgcggaaggc
    13441 aaaacgatgc ttgtctcaac gcacaatctc ggctcagtca cgacattttg cgattatacg
    13501 gtcatggtca aaggcaccgt cctggcaagc gggccgacag acaccacttt tacagccgag
    13561 aacctggagc tggcttttag cggcgtactg cgccatgtca cacttaacga ctcggaagaa
    13621 agcattatta ctgacgatga acgccctttt gtggcacatc gaccgtcagc ggtgcagagg
    13681 gaagcaagat gaacgtgcta ctggaaccct tcagctatga atatatgctc aatgcgatgt
    13741 gggtctcggc aatggtcggc ggtctctgcg ctttcctgtc atgctatctg atgctaaaag
    13801 gctggtcgct gattggcgat gcgctgtcgc actctattgt cccgggtgtt gcgggggcat
    13861 atatgctggg gcttccgttt tctctgggag ccttcttttc cggcggactg gcagcgggca
    13921 gcatgctgtt tcttaaccaa cgcacccgct taaaagaaga tgccatcatc ggcctgatct
    13981 tctcgtcctt ttttggcctg gggctgttta tggtgtcatt gaacccgaca tccgtgaaca
    14041 ttcagaccat cgtcctgggc aatattctgg caatcgatcc ggcggatatc ctgcaactca
    14101 ctatcattgg cattctctcg atcatcgtgc tttttttcaa atggaaagat cttatggtga
    14161 cctttttcga cgagaatcac gcccgtgcta ttggattaca tccgggcaga ttaaaaatcc
    14221 tcttcttcac cctgctgtcg gtttcgaccg ttgcggcgct gcaaacggtt ggtgcctttc
    14281 tggtgatctg tctggtggtg actcccggtg caacagcatg gttgttaacc gatcgttttc
    14341 cccgcctgct cataattgct gtaactattg gcagtgtaac cagtttcctc ggcgcgtggg
    14401 tcagttactt tctggacggc gcgacaggtg gaatcatcgt ggtggcgcag acactgctgt
    14461 ttcttttagc atttgtattt gcgcccaccc atggactttt ggcaaatcgt cggcgtgcgc
    14521 ataaagcgct ggaggatcgc tcatgatggc attattactg gagccactgc agttcacctt
    14581 tatgagtcac gcactgctga tttcactggt agtttccata ccctgtgcgc tgctgtcggt
    14641 ctttctggtc ctgaaaggtt gggcactgat gggtgacgcg atgagtcatg cggtttttcc
    14701 tggcatcgtg cttacctgga ttctggggtt acctctggct acaggggcat tcgtcgccgg
    14761 agtattctgc gcggtggcga cgggatacct gaaagacaac agccgaatca agcaagatac
    14821 agttatgggg attgtttttt ctggcatgtt tgccgcaggt ctgatcttgt atattgcaat
    14881 caaaccagac gtacatcttg accatattct tttcggcgac atgctcggga taaccatcgg
    14941 cgatataatc cagacggtga ttattgccgg gctggttaca cttgttatta gcgcaaaatg
    15001 gcgggatttt ttgctgttca gctttgatta tcaacaggcg caggtaagcg gtttgcatac
    15061 gagatggctg cattatgggc tgctgtgcat ggtttccctg accattgtgg cgacgctgaa
    15121 agcagtaggt atcattctct ctatttctct gctcattgcg cctggcgcca ttgcagtact
    15181 cctgacacaa cgctttcata ttgcgctcct actggcgact ggcatatcgg taatagtgtc
    15241 aatgactggt gtctggctgt ccttttttat cgacagcgca ccagctccga cgattgtcgt
    15301 cttattcgcg gtcgtgttca tcatgacgtt taccgttacc agcatcaacg cacgcacaaa
    15361 gggaaacgcc catacacagg atctgttatc acccaattag cacaacatcc gaggacagca
    15421 tcgtccgcca gcctacagaa gtccggaaag ccatggaagg tcgttgggag gaggtacgaa
    15481 ttctatcatg caaaaaatac gtaaaatcga taacgcctag aaatcattca atactcgcac
    15541 tatcgaaagt ttaccagcca accgcggcac gttcctgcat acgacgtgcc gcagcttctg
    15601 ttattccacg ataaaattcc taaccgaatt caaatccttc agactattca gctcttcctt
    15661 cagctcccgc tgacggcgat aaatctcgtc attgcgctca acctgtgcct gagccattgc
    15721 tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat cggcatcacc
    15781 ccacgtcatc acgtcccgta caatgtcgga tttcgcagcc attaccaccg gataaaggcg
    15841 gcccagtgag ttggggccag cattccagat acgaccgttc cattcaaacg tgaacggctt
    15901 cgcctcctgt tctgtgcgcc atgcttcaat tccctgacgt ctggcctctc tggccgcttc
    15961 cagcatttct ggtgtcacag tgaatggggc tatctcaccc catttgccac tttgcagttc
    16021 ctgccagatt cgctgacccg tcggtgcgac atcatcagcg gtggctgtgt aggggactgc
    16081 ctggtccctg tcgtcaaaaa aaacgtcaca gtctactgcg ccactttcgg tataacgggg
    16141 attaatgatt tttttaattt ccacggt
//
LOCUS       DAQSWQ010000094        16070 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.095, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000094 DAQSWQ010000000
VERSION     DAQSWQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.095"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(1..563)
                     /locus_tag="R8G18_003160"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="R8G18_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(797..2217)
                     /gene="espX1"
                     /locus_tag="R8G18_003161"
                     /pseudo
     CDS             complement(797..2217)
                     /gene="espX1"
                     /locus_tag="R8G18_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="R8G18_003162"
     CDS             complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="R8G18_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEE9796788.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3068..3286
                     /locus_tag="R8G18_003163"
     CDS             3068..3286
                     /locus_tag="R8G18_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HEE9796789.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3294..4235
                     /gene="ribF"
                     /locus_tag="R8G18_003164"
     CDS             3294..4235
                     /gene="ribF"
                     /locus_tag="R8G18_003164"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEE9796790.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4278..7094
                     /gene="ileS"
                     /locus_tag="R8G18_003165"
     CDS             4278..7094
                     /gene="ileS"
                     /locus_tag="R8G18_003165"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEE9796791.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7094..7588
                     /gene="lspA"
                     /locus_tag="R8G18_003166"
     CDS             7094..7588
                     /gene="lspA"
                     /locus_tag="R8G18_003166"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEE9796792.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7713..8162
                     /gene="fkpB"
                     /locus_tag="R8G18_003167"
     CDS             7713..8162
                     /gene="fkpB"
                     /locus_tag="R8G18_003167"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEE9796793.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8164..9114
                     /gene="ispH"
                     /locus_tag="R8G18_003168"
                     /gene_synonym="lytB"
     CDS             8164..9114
                     /gene="ispH"
                     /locus_tag="R8G18_003168"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEE9796794.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9180..10094
                     /gene="rihC"
                     /locus_tag="R8G18_003169"
     CDS             9180..10094
                     /gene="rihC"
                     /locus_tag="R8G18_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HEE9796795.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10261..11082
                     /gene="dapB"
                     /locus_tag="R8G18_003170"
     CDS             10261..11082
                     /gene="dapB"
                     /locus_tag="R8G18_003170"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEE9796796.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11538..12686
                     /gene="carA"
                     /locus_tag="R8G18_003171"
     CDS             11538..12686
                     /gene="carA"
                     /locus_tag="R8G18_003171"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEE9796797.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12704..15925
                     /gene="carB"
                     /locus_tag="R8G18_003172"
     CDS             12704..15925
                     /gene="carB"
                     /locus_tag="R8G18_003172"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEE9796798.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3918 a         3782 c         4258 g         4112 t
ORIGIN      
        1 tccccccagt agaaaggaat ggagataaac agattaaaac gctcctcttc tttattattt
       61 tcatcataag attggctggc agagaaagta taactaatgt gttgccagtt attggagtaa
      121 ctgaattggt aatctttagc atttccactt cgcccccagt aattccgcca aagcgcactt
      181 aatgatacat ttcccagatt attggataaa ggttgcataa tattggcaga aaggctattt
      241 tttctgccaa tatcataaaa atcatcataa tcactctgat gatagtgttt atcattttcg
      301 taaagatggt cgctgaatgt cctgtaatcc tgcgaagcat aacgccaggc ggccacgcta
      361 aatcgagtgg ctgtttgtac tagatattta ttataggcga cctgatagct cgtaccttcg
      421 tgtctggtat cattatttaa cttactactc gatcgggcag cgtaaaaaga gatggcaccc
      481 agcggtgtat tccaaccatt tcccagagtt atagcattat agttatcgga taaaatcgta
      541 cccgattatg tgatcaggtt gttattattt agattaatgt ttaaattttt taatttcttg
      601 atgtgaagtt gatgaaagtt tgaaaattta atattcttaa ttgttccctt ctttataaga
      661 agagcaaggt gccatcatta gagtgacaga gtaggatgtt tcagagcatg cttcagattg
      721 ttttttattt ctctgcagtg tattaataat catcacagat aatttcattc taaaattacc
      781 tctttctttg agtgttttaa tcctgatgaa ctatattttt tataaggagc atgcactcgt
      841 tgatgctctg aaggtatttt ctcatttttg atgatgtttt gtcgtaatcg tgcataaact
      901 tattaagttc tttatacaca tcattattta gctttacatt atcgcgaact aaagtttgta
      961 ccaatggtat caaaatttcg aaacgggacg gtttgtcatc aatacttgct agggttttga
     1021 atactcctgt gtatctgtaa cctaagtgct tattgacaag ttgagtggtc catcggccag
     1081 gcataatatc ttcacagcgc aaatttttta tcatggtatt agtgaacgat gttccaaata
     1141 tgttgctttt tttaatgaag aggttgttta tttctccatt tgaaaagatg gtgtttgata
     1201 agtcgctttc aaaacatgat atattggtta atacacaaca aaaataatac ccatatgaaa
     1261 ggttgctctt ttctattgtt atatttgata gcaacatttt atgtatatta atattaaggg
     1321 cagtcaaata ctggatcttt tgatcgttaa gttcatacat ctccagtgat ttattatagg
     1381 gtaataaaag tataaattcc tttatattat ctccgcagta agaaaagacg acattaaatt
     1441 tattagtggc catgttttca gttgagcagt aagctgattt ttctattaac cgactgggca
     1501 cgcttttaaa taaagataat tttccacata gttttgaata tgccgaattg ctagttttaa
     1561 agttctgcca cattattccc tggttatcat ctatcgatga ggaggggatt ttattgcatt
     1621 gctcattgta tagctcaggg aaaatatttt ttttaataag ctttttgatt tcctggttaa
     1681 gatgactttt tttcttgtct attgcactgt attttgcgtt ttgtgacgtt tcctgatgat
     1741 cataaaatga ctggagattt ctgcaggcct cataaaatat ttcaggagta tatagctgtg
     1801 ggtatatttc ttggttgaag tttgccttta aaaaattaac gaatttttca tggcattctt
     1861 ggtatatctt aatctcttca gaaagacatt cgcttgttct gtcatcaaaa acatccaatc
     1921 taagtaatgt gatacctttg tgtttaaaaa ataaaattga actgtatgtt ctatgagttt
     1981 acatctataa aaatatgcat ctctcagatg agtatattga aaaaaatgat gaaggactgg
     2041 agatggttta ggcaagttat ggcttctaaa aaagtcatca aacaaaatta agaactctgt
     2101 tttatactcg caaaatactt ttactaactc attttgtctt gttttattta ttattacatc
     2161 atctactata tttgatttat aaagacaatg tggcgaagtt gagataccat ttgtcattat
     2221 cagtccttga agttgatgaa tttttattaa tttaatttat gagtgaatgt gactactatc
     2281 aaaaatagct cattaatttt atgaagtaga atataaatag gtatttaacc tgtgttgatt
     2341 gttgttaatt atttacaaga tctaataagg agatatttat gaatggttaa agtgtatagg
     2401 aatttaattc aaaaatagag tggtcttaag tttaatacct caatgtgtat cacaatatcc
     2461 acattctttg taggggagtc tggagattga gtagatattc ttgttcagaa tgtatcagcc
     2521 gatggttcta cgattcttaa gccacgaaga gttcagaaag tgtaacggca tgtctgcaaa
     2581 agccagcatc ttttgactat ctggcaaccg gtagtgtgtt ctctcacgca tcataaaagc
     2641 agcaggcata aaaaaaaccc gcttgcgcgg gctttttcac aaagcttcag caaattggcg
     2701 attaagccag tttgttgatc tgtgcagtca ggttagcctt atgacgtgca gctttgtttt
     2761 tgtggatcag acctttagca gcctgacggt ccacgatcgg ttgcatttcg ttaaatgctt
     2821 tctgtgcagc agctttgtcg ccagcttcga tagctgcgta tactttcttg atgaaagtac
     2881 gcatcataga gcgacggctt gcgttgtgct tacgagcctt ttcagactga atggcgcgct
     2941 tcttagctga tttgatatta gccaaggtcc aactcccaaa tgtgttctat atggacaatt
     3001 caaaggccga ggaatatgcc cttttagcct tcttttgtca atggatttgt gcaaataagc
     3061 gccgttaatg tgccggcact cgttacgtag tgatggcgca ggattctacc agcttgcggg
     3121 gtgtgaatac agcttttccg cgataaaaat tgcagcaggc ggtcactttc ttcccgtgat
     3181 ttgcgccatg gcaatgaaaa gccacttctt tctgatttcg gtactcaatc gccggttaac
     3241 cttgaccgct gtacaaggta tactcggacg attttcactg ttttgagcca gacatgaagc
     3301 tgatacgcgg catacataat ctcagccagg ccccgcaaga agggtgtgtg ctgactattg
     3361 gtaatttcga cggcgtgcat cgcggtcatc gcgcgctgtt acagggcttg caggaagaag
     3421 ggcgcaagcg caacttaccg gtgatggtga tgctttttga acctcaacca ctggaactgt
     3481 ttgctaccga taaagccccg gcaagactga cccggctgcg ggaaaaactg cgttaccttg
     3541 cagagtgtgg cgttgattac gtgctgtgcg tgcgtttcga caggcgtttc gcggcgttaa
     3601 ccgcgcaaaa tttcatcagc gatcttctgg tgaagcattt gcgcgtaaaa tttcttgccg
     3661 taggggatga tttccgcttt ggcgctggtc gtgaaggcga tttcttgtta ttacagaaag
     3721 ctggcatgga atacggcttc gatatcacta gtacgcaaac tttttgcgaa ggtggcgtgc
     3781 gtattagcag caccgctgtg cgtcaggcgc tggcggatga caatctggct ctggcagaaa
     3841 gtttactggg gcacccgttt gccatctccg ggcgtgtagt ccacggtgat gaattagggc
     3901 gcactatagg tttcccgacg gcgaatgtac cgctacgccg tcaggtttcc ccggtgaaag
     3961 gggtttatgc ggtagaagtg ctgggcctcg gtgaaaagcc gttacccggc gtggcaaaca
     4021 tcggaacacg cccaacggtt gccggtattc gccagcagct ggaagtgcat ttgttagatg
     4081 ttgcaatgga cctttacggt cgccatatac aagtagtgct gcgtaaaaaa atacgcaatg
     4141 agcagcgatt tgcgtcgctg gacgaactga aagcgcagat tgcgcgtgat gaattaaccg
     4201 cccgcgaatt ttttgggcta acaaaaccgg cttaagcctg ttatgtaatc aaaccgaaac
     4261 acggaaccga gaatctgatg agtgactata aatcaaccct gaatttgccg gaaacagggt
     4321 tcccgatgcg tggcgatctc gccaagcgcg aaccgggaat gctggcgcgt tggactgatg
     4381 atgatctgta cggcatcatc cgtgcggcta aaaaaggcaa aaaaaccttc attctgcatg
     4441 atggccctcc ttatgcgaat ggcagcattc atattggtca ctcggttaac aagattctga
     4501 aagacattat cgtgaagtcc aaagggcttt ccggttatga ctcgccgtat gtgcctggct
     4561 gggactgcca cggtctgccg atcgagctga aagtagagca agaatacggt aagccgggtg
     4621 agaaattcac cgccgccgag ttccgcgcca agtgccgcga atacgcggcg acccaggttg
     4681 acggtcaacg caaagacttt atccgtctgg gcgtgctggg cgactggtcg cacccgtacc
     4741 tgaccatgga cttcaaaact gaagccaaca tcatccgcgc gctgggcaaa atcatcggca
     4801 acggtcacct gcacaaaggc gcgaagccag ttcactggtg cgttgactgc cgttctgcgc
     4861 tggcggaagc ggaagttgag tattacgaca aaacttctcc gtccatcgac gttgcttttc
     4921 aggcggtcga tcaggatgca ctgaaagcaa aatttgccgt aagcaacgtt aacggcccaa
     4981 tctcgctggt gatctggacc actacgccgt ggactctgcc tgcgaaccgc gcaatctcta
     5041 ttgcacctga tttcgactat gcgctggtgc agatcgacgg tcaggccgtg attctggcga
     5101 aagatctggt tgaaagcgta atgcagcgta tcggcgtgac cgattacacc attctcggca
     5161 cggtaaaagg tgcggagctt gagttgctgc gctttaccca tccgtttatg ggcttcgacg
     5221 ttccggcaat cctcggcgat cacgttaccc tagatgccgg taccggtgcc gttcacaccg
     5281 cgcctggcca cggcccggac gactatgtga tcggtcagaa atacggcctg gaaaccgcta
     5341 acccggttgg cccggacggc acttatctgc cgggcactta tccgacgctg gatggcgtga
     5401 acgtcttcaa agcgaacgac atcgtcgttg cgctgctgca ggaaaaaggc gcgctgctgc
     5461 acgttgagaa aatgcagcac agctatccgt gctgctggcg tcacaaaacg ccgatcatct
     5521 tccgcgcgac gccgcagtgg ttcgtcagca tggatcagaa aggtctgcgt gcgcagtcac
     5581 tgaaagagat caaaggcgtg cagtggatcc cggactgggg ccaggcgcgt atcgagtcga
     5641 tggttgctaa ccgtcctgac tggtgtatct cccgtcagcg cacctggggc gtaccgatgt
     5701 cactgttcgt gcacaaagac acggaagagc tgcatccgcg tactctcgaa ctaatggaag
     5761 aagtggcaaa acgcgttgaa gttgacggca tccaggcgtg gtgggatctt gatgcgaaag
     5821 agatcctcgg cgatgaagct gatcagtacg tgaaagtgcc ggacacattg gatgtatggt
     5881 ttgactccgg atctacccac tcttctgttg ttgacgtgcg tccggaattt gccggtcacg
     5941 cagcggacat gtatctggaa ggttctgacc agcaccgtgg ttggttcatg tcttccctaa
     6001 tgatctccac cgcgatgaaa ggcaaagcgc cgtatcgtca ggtactgacc cacggcttta
     6061 ccgtggatgg tcagggtcgc aagatgtcta aatccatcgg caataccgtt tcgccgcagg
     6121 atgtgatgaa caaactgggc gcggatattc tgcgtctgtg ggtggcatca accgactaca
     6181 ccggtgaaat ggccgtttct gacgagatcc tgaaacgtgc tgccgatagc tatcgtcgta
     6241 tccgtaacac cgcgcgcttc ctgctggcaa acctgaacgg ttttgatcca gcaaaagata
     6301 tggtgaaacc ggaagagatg gtggtactgg atcgctgggc cgtaggttgt gcgaaagcgg
     6361 cacaggaaga catcctcaag gcgtacgaag catacgattt ccacgaagtg gtacagcgtc
     6421 tgatgcgctt ctgctccgtt gagatgggtt ccttctacct cgacatcatc aaagaccgtc
     6481 agtacaccgc caaagcggac agtgtggcgc gtcgtagctg ccagactgcg ctgtatcaca
     6541 tcgcagaagc gctggtgcgc tggatggcac caatcctctc cttcaccgct gatgaagtgt
     6601 ggggctacct gccgggcgaa cgtgaaaaat acgtcttcac cggtgagtgg tacgaaggcc
     6661 tgtttggcct ggcagacagt gaagcgatga acgatgcgtt ctgggacgag ctgttgaaag
     6721 tgcgtggcga agtgaacaaa gtcattgagc aagcgcgtgc cgacaagaaa gtgggtggct
     6781 cgctggaagc ggcagtaacc ttgtatgcag aaccggaact gtcggcgaaa ctgaccgcgc
     6841 tgggcgatga attacgattt gtcctgttga cctccggcgc taccgttgca gactataacg
     6901 acgcacctgc tgatgctcag cagagcgaag tactcaaagg gctgaaagtc gcgttgagta
     6961 aagccgaagg tgagaagtgc ccacgctgct ggcactacac ccaggatgtc ggcaaggtgg
     7021 cggaacacgc agaaatctgc ggccgctgtg tcagcaacgt cgccggtgac ggtgaaaaac
     7081 gtaagtttgc ctgatgagtc aatcgatctg ttcaacaggg ctacgctggc tgtggctggt
     7141 ggtagtcgtg ctgattatcg atctgggcag caaatacctg atcctccaga actttgctct
     7201 gggggatacg gtcccgctgt tcccgtcgct taatctgcat tatgcgcgta actatggcgc
     7261 ggcgtttagt ttccttgccg atagcggcgg ctggcagcgt tggttctttg ccggtattgc
     7321 gattggtatt agcgtgatcc tggcagtgat gatgtatcgc tcgaaggcca cgcagaagct
     7381 aaacaatatc gcttacgcgc tgattattgg cggcgcgctg ggcaacctgt tcgaccgcct
     7441 gtggcacggc ttcgttgtcg atatgatcga cttctacgtc ggcgactggc acttcgccac
     7501 cttcaacctt gccgatactg ccatctgtgt cggtgcggca ctgattgtgc tggaaggttt
     7561 tttgccttct agagcgaaaa aacaataata aaccctgccg gatgcgatgc tgacgcatct
     7621 tatccggcct acagattgct gcgaaatcgt aggccggata aggcgtttac gccgcatccg
     7681 gcaaaaatcc tcaaatataa gagcaaacct gcatgtctga atctgtacag agcaatagcg
     7741 ccgtcctggt gcacttcacg ctaaaactcg acgatggcac caccgccgag tctacccgca
     7801 acaacggtaa accggcgctg ttccgcctgg gtgatgcttc tctttctgaa gggctggagc
     7861 aacacctgtt ggggctgaaa gtgggcgata aaaccacctt ctcgttggag ccagatgcgg
     7921 cgtttggcgt gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg
     7981 caggcgagcc agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc
     8041 ctggcgtgat ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg
     8101 ccgggcagac cgttcatttt gatattgaag tgctggaaat cgatccggca ttggaggcgt
     8161 aacatgcaga tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc
     8221 agcattgttg aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg
     8281 gtacataacc gctatgtggt cgatagcttg cgtgagcgtg gggctatctt tattgagcag
     8341 attagcgaag taccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg
     8401 gtacgtaacg aagcaaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg
     8461 accaaagtgc atatggaagt cgcccgcgcc agccgtcgtg gcgaagagtc gattctcatc
     8521 ggccacgccg ggcatccgga agtggaaggt acaatgggcc agtacagtaa cccggaaggg
     8581 ggaatgtatc tggtcgaatc gccggacgat gtgtggaaac tgacggtcaa aaacgaagag
     8641 aagctctcct ttatgaccca gaccacgctg tcggtggatg acacgtctga tgtgatcgac
     8701 gcgctgcgta aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc
     8761 acgactaacc gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg
     8821 gtcggttcga aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc
     8881 aaacgcgcgt ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt
     8941 aaatgcgtcg gcgtgactgc gggcgcatcg gctccggata ttctggtgca aaatgtggtg
     9001 gcacgtttgc agcagctggg cggtggtgaa gccattccgc tggaaggccg tgaagaaaac
     9061 attgttttcg aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt
     9121 agcagcctaa gttatgcgaa aatgccggtc ttgttaccgg cattttttat ggagaaaaca
     9181 tgcgtttacc tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg
     9241 ccgcgatttt tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct
     9301 cggttgagaa aaccacccgc aacgccctgc aactgctgca tttctggaat gcggagattc
     9361 cgctcgccca gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc
     9421 acggcgaatc gggaatggct ggctacgact ttgttgagca caaccgaaag ccaatcggga
     9481 taccagcgtt tctggcgatt cgtgatgccc tgatgcgtgc accagagcct gttaccctgg
     9541 tggccattgg cccgttaacc aatattgcgc tgttactttc acaatgcccg gaatgtaaac
     9601 cgtatattcg tcgtctggtg atcatgggcg gttcttctgg tcgcggcaac tgtacaccaa
     9661 atgccgagtt taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggaa
     9721 tcgaaatcgt catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc
     9781 tcgctacttt gccggagttg aatcgcaccg gaaaaatgct tcatgcactg tttagccact
     9841 accgtagcgg cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc
     9901 tggtgcgccc ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg
     9961 aatttacctc aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg
    10021 taaaggtggc attggatctg gatgtgaaag gcttccagca gtgggtggct gaggtgctgg
    10081 ctctggcgtc gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt
    10141 tcactgatat ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg
    10201 cgtagcgatg cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct
    10261 atgcatgatg caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag
    10321 ttgattcagg cggcgctggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa
    10381 ggatcttctt tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt
    10441 accgtgcaaa gcagcctcga tgcggtaaaa gatgattttg atgtgtttat cgattttacc
    10501 cgtccggaag gtacgctgaa ccatctcgct ttttgtcgcc agcatggcaa agggatggtg
    10561 atcggcacta cggggtttga cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat
    10621 attgcgattg tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg
    10681 gagaaagcag ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat
    10741 agacataaag ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac
    10801 gcccttgata aagatctgaa agattgcgcg gtctacagtc gtgaaggcca caccggtgaa
    10861 cgtgtgcctg gcaccattgg ttttgccacc gtgcgtgcag gtgacatcgt tggtgaacat
    10921 accgcgatgt ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgt
    10981 atgacatttg ctaacggcgc ggtaagatcg gctttgtggt tgagtggtaa ggaaaatggt
    11041 ctttttgata tgcgagatgt acttaatctc aataatttgt aaccacaaaa tatttgttat
    11101 ggtgcaaaaa taacacattt aatttattga ttataaaggg ctttaatttt tggccctttt
    11161 atttttagtg ttatgttttt aaatattcta taagtgccaa aaattacatg ttttgtcttc
    11221 tgtttttgtt gttttaatgt aaattttgac catttggtcc actttttcct gctcgttttt
    11281 atttcatgca atcttcttgc tgcgcaagcg ttttccagaa caggttatat gatctttttg
    11341 tcgcttaatg cctgtaaaac atgcatgagc cacaaaataa tataaaaaat accgccatta
    11401 agttgacttt tagcgcccat atctccagaa tgccgccgtt tgccagaaat tcgtcggtaa
    11461 gcagatttgc attgatttac gtcatcattg tgaattaata tgcaaataaa gtgagtgaat
    11521 attctctgga gggtgttttg attaagtcag cgctattggt tctggaagac ggaacccagt
    11581 ttcacggtcg ggccataggg gcaacaggtt cggcggttgg ggaagtcgtt ttcaatactt
    11641 caatgaccgg ttatcaagaa atcctcactg atccttccta ttctcgtcaa atcgttactc
    11701 ttacttatcc ccatattggc aatgtcggca ctaatgacgc cgatgaagaa tcttctcagg
    11761 tacatgcaca aggtctggtg attcgcgatc tgccgctgat tgccagcaac ttccgtaata
    11821 ccgaagacct ctcttcttac ctgaagcgcc ataacatcgt ggcgattgcc gatatcgata
    11881 cccgtaagct gacgcgttta ctgcgcgaga aaggcgcaca gaatggctgc attatcgcag
    11941 gcgataaccc ggatgcggcg ttggcgttag aaaaagcccg cgcgttccca ggtctgaatg
    12001 gcatggatct ggcaaaagaa gtgaccaccg cagaagccta tagctggaca caagggagct
    12061 ggacgttgac cggcggcctg ccagaagcga aaaaagaaga cgagctgccg ttccacgtcg
    12121 tggcttatga ttttggtgcc aagcgcaaca tcctgcggat gctggtggat agaggctgtc
    12181 gcctgaccat cgttccggcg caaacttctg cggaagatgt gctgaaaatg aatccagacg
    12241 gcatcttcct ctccaacggt cctggcgacc cggccccgtg cgattacgcc attaccgcca
    12301 tccagaaatt cctcgaaacc gatattccgg tattcggcat ctgcctcggt catcagctgc
    12361 tggcgctggc gagcggtgcg aagactgtca aaatgaaatt tggtcaccac ggcggcaacc
    12421 atccggttaa agatgtggag aaaaacgtgg tgatgatcac cgcccagaac cacggttttg
    12481 cggtggatga agcaacatta cctgcaaacc tgcgtgtcac gcataaatcc ctgttcgacg
    12541 gtacgttaca gggcattcat cgcactgata aaccggcgtt cagcttccag ggtcacccgg
    12601 aagccagccc tgggccacac gacgcagcgc cgttgttcga ccactttatc gagttaattg
    12661 agcagtaccg taaaaccgct aagtaatcag gagtaaaaga gccatgccaa aacgtacaga
    12721 tataaaaagt atcctgattc tgggtgcggg cccgattgtt atcggtcagg cgtgtgagtt
    12781 tgactactct ggcgcgcaag catgtaaagc cctgcgcgaa gagggttacc gcgtcattct
    12841 ggtgaactcc aacccggcga ccatcatgac cgacccggaa atggccgatg cgacctacat
    12901 cgagccgatt cactgggaag tagtacgcaa gattattgaa aaagagcgcc cggacgcggt
    12961 gctgccaacg atgggcggtc agacggcgct gaactgcgcg ctggagctgg agcgtcaggg
    13021 cgtgttggaa gagttcggcg tgactatgat tggtgcgacc gccgatgcga ttgataaagc
    13081 agaagaccgc cgtcgtttcg acgtagcgat gaagaaaatt ggtctggaaa ccgcgcgttc
    13141 cggtatcgca cacacgatgg aagaagcgct ggcggttgcc gctgacgtgg gcttcccgtg
    13201 cattattcgc ccatccttta ccatgggcgg tagcggcggc ggtatcgctt ataaccgcga
    13261 agagtttgaa gaaatttgcg cccgcggtct ggatctctcc ccaaccaaag agctgctgat
    13321 tgatgagtcg ctgatcggct ggaaagagta cgagatggaa gtggtgcgtg ataaaaacga
    13381 caactgcatc atcgtctgct ctatcgaaaa ctttgatgcg atgggcatcc ataccggtga
    13441 ctccatcact gtcgcgccag cccaaacgct gaccgacaaa gaatatcaaa tcatgcgtaa
    13501 cgcctcgatg gcggtgctgc gtgaaatcgg cgttgaaacc ggtggttcca atgtccagtt
    13561 tgcggtgaac ccgaaaaacg gtcgcctgat tgttatcgaa atgaacccac gcgtgtcccg
    13621 ttcttcggcg ctggcgtcga aagcgaccgg tttcccgatt gctaaagtgg cggcgaaact
    13681 ggcggtgggt tacaccctcg acgaactgat gaacgacatc actggcggac gtactccggc
    13741 ctccttcgag ccgtccatcg actatgtggt tactaaaatt cctcgcttca acttcgaaaa
    13801 attcgctggt gctaacgacc gtctgaccac tcagatgaaa tcggttggcg aagtgatggc
    13861 gattggtcgc acgcagcagg aatccctgca aaaagcgctg cgcggcctgg aagtcggtgc
    13921 gactggattc gacccgaaag tgagcctcga tgacccggaa gcgttaacca aaatccgtcg
    13981 cgaactgaaa gatgctggcg cagagcgtat ctggtacatc gccgatgcct tccgtgcggg
    14041 cctgtctgtg gacggcgtgt tcaacctgac caatattgac cgctggttcc tggtacagat
    14101 tgaagaactg gtgcgtctgg aagagaaagt agcggaagtg ggcatcactg gcctgaacgc
    14161 tgaattcctg cgccagctga aacgcaaagg ctttgccgat gcgcgtctgg caaaactggc
    14221 gggcgtgcgc gaagcggaaa tccgcaagct gcgcgaccag tatgacctgc acccggttta
    14281 taagcgcgtg gatacctgtg cggcagagtt cgccaccgac accgcttaca tgtactccac
    14341 ttatgaagaa gagtgcgaag cgaatccgtc taccgaccgt gaaaaaatca tggttctcgg
    14401 cggcggcccg aaccgtatcg gtcagggtat cgaattcgac tactgctgtg tacacgcctc
    14461 gctggcgctg cgcgaagacg gttacgaaac cattatggtt aactgtaacc cggaaaccgt
    14521 ctccaccgac tacgacactt ccgatcgcct ctacttcgag ccggtaactc tggaagatgt
    14581 gctggaaatc gtgcgtattg agaagccgaa aggcgttatc gttcagtacg gcggtcagac
    14641 cccgctgaaa ctggcgcgcg cgctggaagc tgctggcgta ccggttatcg gcaccagccc
    14701 ggatgctatc gaccgtgcag aagaccgtga acgcttccag catgcggttg agcgtctgaa
    14761 actgaaacaa ccggcgaacg ccaccgttac cgctattgaa atggcggtag agaaggcgaa
    14821 agagattggc tacccactgg tggtacgtcc gtcttacgtt ctcggcggtc gggcgatgga
    14881 aatcgtctat gacgaagctg acctgcgtcg ctacttccag acggcggtca gcgtgtctaa
    14941 cgatgcgcca gtgttgctgg atcacttcct tgatgacgcg gtagaagttg acgtggatgc
    15001 catctgcgac ggcgaaatgg tgctgattgg cggcatcatg gagcacatcg agcaggcggg
    15061 cgtgcactcc ggtgactccg catgttctct gccagcctac accttaagtc aggaaattca
    15121 ggatgtgatg cgccagcagg tgcagaaact ggccttcgaa ttgcaggtgc gcggtctgat
    15181 gaacgtgcag tttgcggtga aaaacaacga agtctacctg attgaagtta acccgcgtgc
    15241 ggcgcgtacc gttccgttcg tctccaaagc caccggcgta ccgctggcaa aagtggcggc
    15301 gcgtgtgatg gctggcaaat cgctggctga gcagggcgta accaaagaag ttatcccgcc
    15361 gtactactcg gtgaaagaag tggtgctgcc gttcaataaa ttcccgggtg ttgacccgct
    15421 gttagggcca gaaatgcgct ccaccgggga agtgatgggc gtgggccgca ccttcgctga
    15481 agcgtttgcc aaagcgcagc tgggcagcaa ctccaccatg aagaaacacg gtcgtgcgtt
    15541 gctttccgtg cgcgaaggcg ataaagaacg cgtggtggac ctggcggcaa aactgctgaa
    15601 acagggcttc gagctggatg cgacccacgg cacggcgatt gtgctgggcg aagcgggcat
    15661 taatccgcgt ctggtgaaca aggtgcatga aggccgtccg cacattcagg accgcatcaa
    15721 gaatggcgaa tatacctaca tcatcaacac cacttcaggc cgtcgcgcga ttgaagactc
    15781 ccgcgtgatc cgtcgcagtg cgctgcaata taaagtgcat tatgacacca ccctgaacgg
    15841 tggtttcgct accgcgatgg cgctgaatgc cgacgcgacc gaaaaagtaa tttcggtgca
    15901 ggaaatgcac gcgcagatca aataatagcg tgtcatggcg gatatttttc atccgctaat
    15961 ttgatcgaat aagtaatacg gttctctgat gaggaccgtt ttttttgccc attaagtaaa
    16021 tcttttgggg aatcgatatt tttgatgaca taagcaggat ttagctcaca
//
LOCUS       DAQSWQ010000095        16044 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.096, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000095 DAQSWQ010000000
VERSION     DAQSWQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.096"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(36..1859)
                     /gene="typA"
                     /locus_tag="R8G18_003173"
     CDS             complement(36..1859)
                     /gene="typA"
                     /locus_tag="R8G18_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HEE9796799.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2232..3641
                     /gene="glnA"
                     /locus_tag="R8G18_003174"
     CDS             2232..3641
                     /gene="glnA"
                     /locus_tag="R8G18_003174"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HEE9796800.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3815..4864
                     /gene="glnL"
                     /locus_tag="R8G18_003175"
     CDS             3815..4864
                     /gene="glnL"
                     /locus_tag="R8G18_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEE9796801.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4876..6285
                     /gene="glnG"
                     /locus_tag="R8G18_003176"
     CDS             4876..6285
                     /gene="glnG"
                     /locus_tag="R8G18_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEE9796802.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6397..6507
                     /locus_tag="R8G18_003177"
     CDS             6397..6507
                     /locus_tag="R8G18_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HEE9796803.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="R8G18_003178"
     CDS             complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="R8G18_003178"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEE9796804.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="R8G18_003179"
     CDS             complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="R8G18_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HEE9796805.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            9350..9982
                     /locus_tag="R8G18_003180"
     CDS             9350..9982
                     /locus_tag="R8G18_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEE9796806.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10364..13150)
                     /gene="polA"
                     /locus_tag="R8G18_003181"
     CDS             complement(10364..13150)
                     /gene="polA"
                     /locus_tag="R8G18_003181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEE9796807.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13514..14441
                     /locus_tag="R8G18_003182"
                     /pseudo
     CDS             13514..14441
                     /locus_tag="R8G18_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(14482..15912)
                     /locus_tag="R8G18_003183"
     CDS             complement(14482..15912)
                     /locus_tag="R8G18_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEE9796808.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3803 a         4094 c         3940 g         4207 t
ORIGIN      
        1 agcgatggca ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
       61 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
      121 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
      181 ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
      241 ccggtcaggc agtttacagt caggtcgtta gagcggctgt gaataccgat aatctgacct
      301 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc ggtcctgcag accgaacagt
      361 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
      421 acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg
      481 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
      541 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
      601 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
      661 tacggctctt gtttacgacc gtcgatttca cggaagatta cttttggacg ggataccgcc
      721 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
      781 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
      841 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
      901 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
      961 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
     1021 ccaaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
     1081 agaccgaggt ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg
     1141 atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
     1201 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
     1261 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
     1321 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
     1381 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
     1441 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
     1501 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
     1561 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
     1621 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
     1681 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
     1741 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
     1801 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
     1861 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
     1921 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
     1981 ggagcataaa aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt
     2041 gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc
     2101 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta
     2161 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg
     2221 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
     2281 ttgttgattt gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc
     2341 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg
     2401 gctggaaagg cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga
     2461 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
     2521 ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc
     2581 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
     2641 atgacatccg tttcggatca tctatctctg gttcccacgt tgctatcgac gatatcgaag
     2701 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
     2761 aaggcggtta cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt
     2821 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
     2881 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
     2941 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
     3001 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta
     3061 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
     3121 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
     3181 ccaactctta taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg
     3241 cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
     3301 tcgaagtacg tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga
     3361 tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
     3421 tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
     3481 aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg
     3541 acgaagcaat tgatgcgtac atcgctctac gtcgcgaaga agatgaccgc gtgcgtatga
     3601 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
     3661 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
     3721 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
     3781 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
     3841 tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat
     3901 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
     3961 tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag
     4021 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc
     4081 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
     4141 tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc agcaggttgc
     4201 tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gaggtttacg
     4261 tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg aatataccaa
     4321 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
     4381 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtgacgct
     4441 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc ccagcctgcc
     4501 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
     4561 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtactcgca ccgcgtttca
     4621 actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg
     4681 gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
     4741 aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa
     4801 aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa
     4861 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
     4921 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac
     4981 ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
     5041 cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg
     5101 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
     5161 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt
     5221 gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc
     5281 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
     5341 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
     5401 ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg
     5461 aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc
     5521 gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggtaca
     5581 ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
     5641 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgt
     5701 attatcgctg ccactcacca gaatctcgaa caacgagtgc aggaaggtaa gttccgtgag
     5761 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg
     5821 gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta
     5881 gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc
     5941 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa
     6001 gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg
     6061 caaatgcaac cggacagctg ggcaacgctt cttgcgcagt gggcagacag agcgctgcgt
     6121 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
     6181 accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc
     6241 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac agcttgtgtg
     6301 taaagattga ttattgagcg caaattgctg gtattttacg ctttactgtt ccgataagtt
     6361 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
     6421 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt
     6481 gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
     6541 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
     6601 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
     6661 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg agaactgctg
     6721 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
     6781 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
     6841 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
     6901 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
     6961 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
     7021 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
     7081 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
     7141 gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
     7201 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
     7261 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
     7321 agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg ggttcagctc
     7381 cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat
     7441 caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca gtgcaaagat
     7501 gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
     7561 gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat ccagttcgat
     7621 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
     7681 cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc
     7741 ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
     7801 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
     7861 accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
     7921 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
     7981 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
     8041 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
     8101 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
     8161 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
     8221 gttatcagca gaacgggaag gcccacggtg gcaatccgtt agttaccccg cagcagacgc
     8281 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
     8341 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
     8401 gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
     8461 tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
     8521 tgtttggtga ctttttcagc cacgcccaat ggaatagggg ttttactgcc aatacgtgga
     8581 tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg
     8641 cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc ctgatccagc
     8701 tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt tgaagatgat
     8761 ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta
     8821 acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca
     8881 taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt tttcctttag
     8941 ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc
     9001 tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct
     9061 gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc
     9121 gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg atttatatag
     9181 cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg
     9241 tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta
     9301 tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt tgactaattt
     9361 gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga
     9421 taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa
     9481 cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc gcacccagct
     9541 tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta
     9601 tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga
     9661 aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc cgctgaaaga
     9721 tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc tggtgctgct
     9781 gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga
     9841 agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca
     9901 aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc agcctgtaga
     9961 agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat gtggcgtatc
    10021 cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat agcgcatcag
    10081 gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact gactggggcg
    10141 gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct
    10201 tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt
    10261 tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc
    10321 ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc
    10381 agttttcgcc actccccact tccaccagca acggcacatc cagacgggta cagttttcca
    10441 tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact tcaaatacca
    10501 gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat
    10561 caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa
    10621 tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat
    10681 acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc
    10741 gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg
    10801 cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca
    10861 gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca
    10921 aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca
    10981 gcaagccttt gtcacgcgaa agatgcgcca taatgcgcag ttcaatctgc gagtagtccg
    11041 ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt
    11101 cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag
    11161 ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca
    11221 gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg
    11281 gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac
    11341 ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct
    11401 tggtggaaga aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag
    11461 ccagacgaag ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac
    11521 cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc tcgaagacgt
    11581 tcaacggccc tttgtgtttt tgcagatctg gccacatttt cagatgcaac tgcaaggtga
    11641 catccgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca
    11701 gttgattttt gcctttaccc gcaatctctt caaaagtgat agttttgtgc ttcaaccaac
    11761 gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca
    11821 gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat
    11881 cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca
    11941 actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg
    12001 gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagcagaga
    12061 tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg
    12121 caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca
    12181 ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc ttcgcggctg
    12241 gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga
    12301 actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc acttccagtt
    12361 gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat
    12421 aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca
    12481 acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca
    12541 atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatcg gaggagtcac
    12601 ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct
    12661 cttccggtcc gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca
    12721 gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt
    12781 cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca
    12841 gtcccatcgc tttaaccatc gcgtgcaggg gttcgatttg tgctcgcaga tcgtccggca
    12901 ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg
    12961 cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt
    13021 tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg
    13081 cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga
    13141 tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg
    13201 atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc
    13261 tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt
    13321 atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaagcca
    13381 tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgga ttatttttct
    13441 ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt
    13501 tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc
    13561 gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc
    13621 gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt
    13681 ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca
    13741 tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg
    13801 gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt
    13861 tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt
    13921 tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta
    13981 tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga
    14041 aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca
    14101 agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa aggcggcagg
    14161 cattgcgatg gcgctaaatg tataaacaat tcgataaact gcttaatgtt acgctgtgtt
    14221 atccggacaa taaccgtcag ccgttcttcg atatgttaag cggtaaactg acgcggattg
    14281 tcgtacatgt ggatttacag cctattgccg atgagttaca tggcgactac atcaatgaca
    14341 aaagctttaa gcgccatttt cagcaatggc tgaactcatt gtggcaggaa aaagaccggc
    14401 ttctgacgtc tctcatgtca tcacagcgtc agaataagta agttttactc gcctctgaat
    14461 gagcagaggc gagtgagtat tttaatgaac aaaacgtccg gcacgagaca taaattcttc
    14521 ttcgctcatc tcctgtccgt taaacacaac tttacccggt gtataacgca gctgcagcga
    14581 ggcggtattg ttgtcaatcg tgattaactg aaacatctga cccaatgtca tcatcccgct
    14641 gatttgttta tcagcctgct tttgcgcttt ttccgcatcc attccttcag ataaattaag
    14701 ctgttttgcg gtttctgtga cgacattaag cggtaacttc acatcaaaat tgagcaattt
    14761 gatatctttg tttgtggatg atgaagattt ggctgggtcg gcaatactga tatccagatt
    14821 ggcatttagt tcgccgagtg cgttcttcca tcttaccggt tgtttaatgg tcggctcact
    14881 tttttgtaat aacggtaaat actctttgaa caatgcagca ttcacttctt gcagagcaac
    14941 ttcatcgttt gctaactcag ggtgtacagc aagctgcttc tgcatagcaa tgttatactg
    15001 gatgataaat tggcgcactg ctgagggatc aatagattca gcaatcaatg accactcacc
    15061 actaccgagt tgctgattcc cttttgttaa cttatcaagt tcgtaggtca gattgacatt
    15121 gatataatct ttgtctttaa cgcgatccag catggttttt gcaacgaaag catcgatttg
    15181 ggcaacgtct tccccgtgat tgataatgtt aatctgatta aatttcgact cgctttcacc
    15241 aaccggaaat ttcttttctt ctaatcgcgc cagagaatca atggtaaaac tttttgccgt
    15301 tactttgttc attggtgata attgcagaac aatatcttcg actttgcctt caatatccag
    15361 gttagacaat gaactaccat caccattaaa agtaaactgg ccctcaccaa aagaaaactt
    15421 tccatactct gcaggggcaa catctaatgt cgtagagaat gagccaccaa acgcgaatcg
    15481 caatgtcgct tccacgggcg atttattttt cgcggcgata aatagcggtt gtgtcagttc
    15541 attattcact aagttcactt ttgccgctgc cagtgctggg atgacattac catgcattaa
    15601 cattgtgatg ggcaacggac cgtgctcaac atccacatca aatacaactt tctggcctgg
    15661 cttgatattg agatcgggtg caccgttatc gaatgtcatt tgcatttgaa aacgagaatt
    15721 aaaaaaacct ttgtcaaaat ttttataact taacgtcata tcgtaggcat gttcaccttt
    15781 ctttttagca tcgttaaaat ctttaataaa cttttcgaca ccaggctgaa tttgcgtacc
    15841 tgtgtaccat gtgccaccac cccagattac ggctaacgca acaataacac ctgtagctga
    15901 cttacgtatc atataaatat tccctgataa aacagcaaaa ctttaaatat ccgcttatgc
    15961 tgaaataatg tccagcggca ggacgcatta tcagaatgta tttatattta ttaaataata
    16021 aaaaaaaccg tgaatattca cggg
//
LOCUS       DAQSWQ010000096        15961 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.097, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000096 DAQSWQ010000000
VERSION     DAQSWQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.097"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="R8G18_003184"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="R8G18_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HEE9796809.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="R8G18_003185"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="R8G18_003185"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HEE9796810.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="R8G18_003186"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="R8G18_003186"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEE9796811.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="R8G18_003187"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="R8G18_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="R8G18_003188"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="R8G18_003188"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEE9796812.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="R8G18_003189"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="R8G18_003189"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HEE9796813.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="R8G18_003190"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="R8G18_003190"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEE9796814.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="R8G18_003191"
     CDS             8229..8939
                     /locus_tag="R8G18_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEE9796815.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="R8G18_003192"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="R8G18_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HEE9796816.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="R8G18_003193"
     CDS             9636..10343
                     /locus_tag="R8G18_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEE9796817.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="R8G18_003194"
     CDS             10343..10891
                     /locus_tag="R8G18_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEE9796818.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="R8G18_003195"
     CDS             10901..12067
                     /locus_tag="R8G18_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEE9796819.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="R8G18_003196"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="R8G18_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="R8G18_003197"
     CDS             13576..14229
                     /locus_tag="R8G18_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEE9796820.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="R8G18_003198"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="R8G18_003198"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HEE9796821.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="R8G18_003199"
     CDS             complement(15612..>15961)
                     /locus_tag="R8G18_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEE9796822.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3699 a         4344 c         4164 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatggc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctagatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag atcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAQSWQ010000097        15881 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.098, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000097 DAQSWQ010000000
VERSION     DAQSWQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.098"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..1453
                     /locus_tag="R8G18_003200"
                     /pseudo
     CDS             1..1453
                     /locus_tag="R8G18_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="R8G18_003201"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="R8G18_003201"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HEE9796823.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="R8G18_003202"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="R8G18_003202"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HEE9796824.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="R8G18_003203"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="R8G18_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HEE9796825.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="R8G18_003204"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="R8G18_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HEE9796826.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="R8G18_003205"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="R8G18_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HEE9796827.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="R8G18_003206"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="R8G18_003206"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEE9796828.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="R8G18_003207"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="R8G18_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEE9796829.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="R8G18_003208"
     CDS             complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="R8G18_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HEE9796830.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8572..9174
                     /locus_tag="R8G18_003209"
     CDS             8572..9174
                     /locus_tag="R8G18_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HEE9796831.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="R8G18_003210"
     CDS             complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="R8G18_003210"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEE9796832.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9732..10472)
                     /locus_tag="R8G18_003211"
     CDS             complement(9732..10472)
                     /locus_tag="R8G18_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEE9796833.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="R8G18_003212"
     CDS             complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="R8G18_003212"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEE9796834.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="R8G18_003213"
     CDS             complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="R8G18_003213"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEE9796835.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13153..13719
                     /locus_tag="R8G18_003214"
     CDS             13153..13719
                     /locus_tag="R8G18_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEE9796836.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14074..14322
                     /locus_tag="R8G18_003215"
     CDS             14074..14322
                     /locus_tag="R8G18_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HEE9796837.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14923..>15881
                     /locus_tag="R8G18_003216"
     CDS             14923..>15881
                     /locus_tag="R8G18_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HEE9796838.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT         4036 a         4030 c         3643 g         4172 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtgtag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata
     8521 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
     8581 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta
     8641 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
     8701 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
     8761 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
     8821 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
     8881 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
     8941 gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg
     9001 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
     9061 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg
     9121 attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac
     9181 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga
     9241 cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg
     9301 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
     9361 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
     9421 ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt
     9481 tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg
     9541 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     9601 atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca
     9661 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     9721 agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact
     9781 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     9841 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
     9901 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg
     9961 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
    10021 ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag
    10081 gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca
    10141 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    10201 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    10261 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    10321 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    10381 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    10441 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    10501 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    10561 cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg
    10621 gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    10681 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    10741 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
    10801 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    10861 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    10921 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    10981 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    11041 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    11101 aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    11161 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    11221 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    11281 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    11341 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    11401 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    11461 ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    11521 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    11581 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
    11641 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    11701 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    11761 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    11821 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    11881 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    11941 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    12001 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    12061 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    12121 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    12181 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    12241 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    12301 acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    12361 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    12421 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    12481 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    12541 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    12601 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    12661 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    12721 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    12781 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    12841 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    12901 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    12961 tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc
    13021 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    13081 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    13141 tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta
    13201 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
    13261 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt
    13321 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct
    13381 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
    13441 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
    13501 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
    13561 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
    13621 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
    13681 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
    13741 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
    13801 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
    13861 ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg
    13921 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
    13981 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
    14041 tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa
    14101 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
    14161 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
    14221 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
    14281 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
    14341 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
    14401 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
    14461 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
    14521 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
    14581 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
    14641 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct
    14701 cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag
    14761 aagttcttca atatataaca tatggagtta atatgcttac acaaactata tatccgcctc
    14821 ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa
    14881 gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac
    14941 aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat
    15001 gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga
    15061 gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg
    15121 tctgaattag gcctgacaac attacctgaa atccccccgg gaattaaatc aattaatata
    15181 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc
    15241 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
    15301 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
    15361 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
    15421 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
    15481 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
    15541 atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt
    15601 gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca
    15661 atagatatta gtgataaccc attgtcatcc catgctctgc aatccctgca aagattaacc
    15721 tcttcgccgg actaccacgg cccgcagatt tacttctcca tgagtgacgg acaacagaat
    15781 acactccatc gccccctggc tgatgccgtg acagcatggt tcccggaaaa caaacaatct
    15841 gatgtatcac agatatggca tgcttttgaa catgaagagc a
//
LOCUS       DAQSWQ010000098        15511 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.099, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000098 DAQSWQ010000000
VERSION     DAQSWQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.099"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            202..474
                     /locus_tag="R8G18_003217"
     CDS             202..474
                     /locus_tag="R8G18_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HEE9796839.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="R8G18_003218"
     CDS             567..650
                     /locus_tag="R8G18_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796840.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="R8G18_003219"
     CDS             750..1571
                     /locus_tag="R8G18_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEE9796841.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="R8G18_003220"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="R8G18_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HEE9796842.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="R8G18_003221"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="R8G18_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HEE9796843.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="R8G18_003222"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="R8G18_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HEE9796844.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="R8G18_003223"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="R8G18_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEE9796845.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="R8G18_003224"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="R8G18_003224"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEE9796846.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /locus_tag="R8G18_003225"
     CDS             7217..8161
                     /locus_tag="R8G18_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEE9796847.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="R8G18_003226"
     CDS             8347..9729
                     /locus_tag="R8G18_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEE9796848.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="R8G18_003227"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="R8G18_003227"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HEE9796849.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="R8G18_003228"
     CDS             complement(10485..10820)
                     /locus_tag="R8G18_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HEE9796850.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="R8G18_003229"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="R8G18_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HEE9796851.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="R8G18_003230"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="R8G18_003230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HEE9796852.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="R8G18_003231"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="R8G18_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HEE9796853.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="R8G18_003232"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="R8G18_003232"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEE9796854.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3812 a         4021 c         3793 g         3885 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAQSWQ010000099        15407 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.100, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000099 DAQSWQ010000000
VERSION     DAQSWQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.100"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            11..454
                     /locus_tag="R8G18_003233"
     CDS             11..454
                     /locus_tag="R8G18_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEE9796855.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="R8G18_003234"
                     /pseudo
     CDS             670..759
                     /locus_tag="R8G18_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="R8G18_003235"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="R8G18_003235"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HEE9796856.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="R8G18_003236"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="R8G18_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEE9796857.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="R8G18_003237"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="R8G18_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEE9796858.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="R8G18_003238"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="R8G18_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HEE9796859.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="R8G18_003239"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="R8G18_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HEE9796860.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="R8G18_003240"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="R8G18_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HEE9796861.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="R8G18_003241"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="R8G18_003241"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEE9796862.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="R8G18_003242"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="R8G18_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEE9796863.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="R8G18_003243"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="R8G18_003243"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEE9796864.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="R8G18_003244"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="R8G18_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HEE9796865.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="R8G18_003245"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="R8G18_003245"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEE9796866.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="R8G18_003246"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="R8G18_003246"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEE9796867.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="R8G18_003247"
     CDS             13056..13436
                     /locus_tag="R8G18_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HEE9796868.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="R8G18_003248"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="R8G18_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HEE9796869.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="R8G18_003249"
     CDS             complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="R8G18_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEE9796870.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15407)
                     /gene="nadA"
                     /locus_tag="R8G18_003250"
     CDS             complement(15245..>15407)
                     /gene="nadA"
                     /locus_tag="R8G18_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEE9796871.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4044 c         3851 g         3596 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat ataacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcag
//
LOCUS       DAQSWQ010000100        15332 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.101, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000100 DAQSWQ010000000
VERSION     DAQSWQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.101"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..357
                     /gene="mcbR"
                     /locus_tag="R8G18_003251"
     CDS             <1..357
                     /gene="mcbR"
                     /locus_tag="R8G18_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HEE9796872.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
     gene            complement(393..2495)
                     /locus_tag="R8G18_003252"
     CDS             complement(393..2495)
                     /locus_tag="R8G18_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEE9796873.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2737..3798
                     /locus_tag="R8G18_003253"
     CDS             2737..3798
                     /locus_tag="R8G18_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEE9796874.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="R8G18_003254"
     CDS             complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="R8G18_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HEE9796875.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5679..6296
                     /locus_tag="R8G18_003255"
     CDS             5679..6296
                     /locus_tag="R8G18_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEE9796876.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            6372..6584
                     /locus_tag="R8G18_003256"
     CDS             6372..6584
                     /locus_tag="R8G18_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HEE9796877.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            7405..9513
                     /locus_tag="R8G18_003257"
     CDS             7405..9513
                     /locus_tag="R8G18_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEE9796878.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRI
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            9581..13123
                     /locus_tag="R8G18_003258"
                     /pseudo
     CDS             9581..13123
                     /locus_tag="R8G18_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            14104..15072
                     /locus_tag="R8G18_003259"
                     /pseudo
     CDS             14104..15072
                     /locus_tag="R8G18_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         4049 a         3674 c         4112 g         3497 t
ORIGIN      
        1 ctcaccccgc aagacctgac ggaactccag gaattgctcg agaaattaca gcaggcgcaa
       61 gaaaagggcg acatggaaca aatcattaat gtaaacaggc tatttcgctt agcgatttat
      121 catcgctcaa atatgcccat cctgtgtgag atgattgagc aactgtgggt caggatgggg
      181 cctggtttac attatctcta tgaagcgatt aatccagcgg aattacggga gcatatagaa
      241 aactatcatc tgttactcgc tgcgttaaaa gcaaaagaca aagagggatg cagacattgt
      301 cttgctgaaa ttatgcaaca aaatattgct attttatacc agcaatacaa tcgttaaagt
      361 aaatgcccgg caaataccgg gcatcaaggt aattactcaa atctccacgc aatattcatg
      421 ccgacaccat aatttcgtcc gggcgaaggt tcgtaatatc gcccgtttga ctcattgaca
      481 atgacagaac caacgtattc tttatcgaat aaattatcga cacgaccaaa taaatccaca
      541 gttaaattgt ggtaattgta tttatacccg gtgaataagc cgacgagagt ataagacggc
      601 gcttttgccg tattttcatc atctgccata atatcgccca tataacgcgc ttccgtgcct
      661 gcataccaac catcttccgg tacataacct atcgacgcaa agcccatatt acgggcgatc
      721 cctggcatcc gattaccgtt acaatcctgt tcattgcaaa cattgctgcg ataggtcgca
      781 tcaagccagg tccaggacgc gtttacgcga aaatctcctg cgaaacgttg atcccatgcc
      841 agttcagcgc cttgacgacg ggtctttccg gcatttttgt aagtcgtacg cccaccgcta
      901 ctgctatcga caacaatttc atcatcagtg tcggtctgaa acaatgcgag actaagcagc
      961 ccatcaccaa tacgcgtttt actgccgatc tcaattgtat cgttggtgga tggttttaaa
     1021 ccaaagttca taccgctttg cccatcagca cgataagaca gctcattaat cgtcggcgtt
     1081 tcaaaacctc gcccggctgc cagatagata ttccaggcat cggtcattgc atattttaac
     1141 gaaccggcag gtagccattt atgataactg gcatcaccgc tgtcatcgcc gttacccgga
     1201 gtaacgtaat ggtcgttgga atcaaaccac acggagctgt agcgcacgcc agcatccagc
     1261 gacagttttt cgctcagctg ccactgcgtc tgtaaatagg gatcgatgtt ccacatcaga
     1321 ttgcgttcgt cgcgacgcaa ctcacctttt tgcccgtact ccggcatgcc gctattcagg
     1381 cggaagttat tgtagccctt gcggttttca ctcatgtttt cgtagttcag gccggtagtg
     1441 aacgtgaccg gaacgcccag ttcaccacgg tgtgtccagc ggctgtctat cccctggtaa
     1501 tggcgttgca gggtaatcac gccgcccgca tgtgacgggt taagttgtgg tgccatgggt
     1561 attgactggt actgggtcgt ttctcgctct ccggcataca tcatcacact catatcatct
     1621 tgcgcgctca ggctacgctc atagcgcaac ccagcctgag tttgcttgat ggtttttcgc
     1681 gtgtcgtact gttctgcacg aggcgcttgt tgtggattag ccttccattc tgctttggtt
     1741 agcccacccg ggtcatctgc tttgatatcc acactattga aaatcagact taatttgctg
     1801 gcttcatcaa tgcgtacgcc cagtttggca ttggctaaat ttttctgtgc gccactatgg
     1861 tcacgatagc cgtgggtcgt aaaacgcgtg gttgagacgg tgtaatcgac atcgccaggc
     1921 tgtgtgccgt ctcccgttgc gcccgttgct ttcagcccat agcgccagct gccaaaactg
     1981 ccgtagtaac tactggcttc aatggttggt ggctgttgtc cggtctgggt ggtgacattc
     2041 attaccccac cagacgcgtt gccatacagg gcagagaagg ggccacgcag cacttccaca
     2101 ttttgcacac tgcttaaatc gatgttggat gtttgccctt gcccgtcggg catggtggcg
     2161 ggaattccgt ccacatacag gcgaataccg cgaataccgt aagtggagcg ggagccaaat
     2221 ccgcgaatcg acagctgtaa atcttgcgca tagttctgcc ggttttgtac ctgcaaacca
     2281 ggcacgtcgg tcagtgattc ggacaagtta atgcgcggtg ttgccaggcg catctcctcg
     2341 ccatccacca cgcttactgc tgctgggata tccagttctg aaaccacctg cggtgcggca
     2401 ctgacaatca tggtctgttc atcagcggca aaaacaacgg gggaaagggc aagtagtgcg
     2461 ggcaaaacgg tctgtcggac ggaaaaaatc ttcatgaaaa aagccaggtt aagaatggga
     2521 aaacgccgtt atggtaataa aattgtaaat tcatggaaaa cgaaacggca cgatacgtta
     2581 agtaattgag aaaattgtag tcgtaacggc aagaaatgct ccacatttga gaaaataatg
     2641 attaccattc ccatttataa caagagcgta acgatgatta cgcttagcga agcattgtga
     2701 agcagcaaaa atatcggttc atcaaaggga gtcgtcatgc atttacgtca tctgttttca
     2761 tcgcgcctgc gtggttcatt actgttaggt tcattgcttg ttgcttcatc attcagtacg
     2821 caggccgcag aagaaatgct gcgtaaagcg gtaggtaaag gtgcctacga aatggcttat
     2881 agccagcaag aaaacgcgct gtggctcgcc acttcgcaaa gccgcaaact ggataaaggc
     2941 ggcgtggttt atcgtcttga tccggtcact ctggaagtga cgcaggcgat ccataacgat
     3001 ctcaagccgt ttggtgtcac catcaataac acgactcaga cactgtggtt tggtaacact
     3061 gtaaacagcg cggtcacggc gatagatgcc aaaacgggcg aggtgaaagg ccgtctggtg
     3121 ctggatgatc gtaagcgtac ggaagaggtg cgcccgctgc aaccgcgtga gctggtagct
     3181 gacgatgcca cgaacaccgt ttacatcagt ggtattggta aagagagcgt gatttgggtc
     3241 gttgatggcg agaatatcaa actgaaaacc gccatccaga acaccggtaa aatgagtacc
     3301 ggtctggcgc ttgatagcaa aggcaaacgt ctttacacca ctaacgctga cggcgaattg
     3361 attaccatcg acaccgccga caataaaatc ctcagccgta aaaagctgct ggatgacggc
     3421 aaagagcact tctttatcaa cattagcctt gataccgcca ggcagcgtgc atttatcacc
     3481 gattctaaag ccgcagaagt gttagtggtc gatacccgta atggcaatat tctggcgaag
     3541 gttgcagcac cggaatcact ggctgtgctg tttaacccag cgcgtaatga agcctacgta
     3601 acgcatcgtc aggcaggtaa agtcagtgtg attgacgcga aaagctataa agtagtgaaa
     3661 acgttcgata cgccgactca tccaaacagc ctggcgctgt ctgccgatgg caaaacgctg
     3721 tatgtcagtg tgaaacaaaa atccactaaa cagcaggaag ctacccagcc agacgatgtg
     3781 attcgtattg cgctgtaata gatatggcga gggtgaaaac ggagagactt gcctgacttt
     3841 tatcacgccg catgcggcac accttcgtca gtaatctaaa tttgcccggc acgtagccgg
     3901 gcaattttgc aattagctcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
     3961 ttcctcgacg actggcgcag tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca
     4021 gcctataacc agcagaatac cgataatcgg cagcgccgcg atagtataag tcccgttcgg
     4081 gtaatcgaac gccatcagca caaggacact cagtaaaaac agtaatgtca gccaggaagt
     4141 gaagggcgcg ccaggcagtt taaaactgac atccgctgct ttgccttctt taatcgcttt
     4201 acgcaggcgc atctggcaca cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
     4261 gaagttcaac acaatctcaa atacgcgcga cggcaccaga tagttgagga atacgccgac
     4321 gacatacaca accagtgtcg ccagaatccc ggcatacggc acatgctgac gactcatttt
     4381 cgccataaaa ctcggtgcgg aaccgcccat cgccatcgag cgcagaatac gcccggtgca
     4441 gtacagacct gagttcaggc tggagagggc agcggtcagc accacaatgt tcataatgct
     4501 gccgatatat ggcataccca gtttagagaa aaacgtcacg aacggacttt gccccgcctg
     4561 atacgcgctc cacggcaata acataaccag caacaccacg gagccgacgt aaaacaggcc
     4621 aatacgccaa atcacactgt taatggcttt aggcaccatg gtctgcggat ctttacattc
     4681 tccggcagct gtacccacca tttcaatgga ggcaaaagca aacactacgc cctgaatcaa
     4741 caccagcgca ggcagcaaac cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa
     4801 gccagtggtg ttgccatcca gcggctgacc actaccgagg aacactgtac ccacgaccaa
     4861 aaaggtcaca atggcgagca ctttaataag cgcaaaccag aactccatct ccgcaaacca
     4921 tttcacaccg atcatattca tggtgccaac gatggtaagt gcagcgagcg caaagaccca
     4981 ctgcggcacg ccgccaaacg caccccagta atgcatatac agagcgacgg cggtaatatc
     5041 aacaatcccc gtcatcgccc agttgatgaa gtacatccag ccagcaacat aagcggcttt
     5101 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtagcac
     5161 cagctcaccc aatgcacgca gaataaaaaa cgaaaacaag ccacaaatta aataaaccag
     5221 tgccagtgct ggacccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
     5281 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
     5341 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
     5401 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
     5461 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
     5521 atcggcgcag catcctaccc gttatcgata tacgatgcaa aacatcccct tacaatcctg
     5581 aaggggattc atacaactga cgaaaaaatg acaaatactt ttgctggtta acctgtgtac
     5641 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
     5701 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
     5761 tcgttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
     5821 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
     5881 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
     5941 ccgaacgtca gcagtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
     6001 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
     6061 atgttattga atatcgtaaa tcgctttata tctggctgaa ttcacaattg accgctgaac
     6121 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
     6181 ggggggctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctgatg
     6241 ctgtatgtca gttaccgaaa ttacaagaag tattaaaaag gaatgaaatt atttgagtgg
     6301 gataccagag gattatgtta tttattattc agtaacttta tccacaatga tgggcgtaat
     6361 taattaaatt catgttatgt tttttgattt atatcactct ccccttcagt cagcttgtct
     6421 atttcatttc ctctgaaaag aaactaacta ttcacattgt gcagatgttt cacttgttat
     6481 cacaggtatt ctataatctc aaaaagtttt taatgatgga tatgctcgga gttggagatg
     6541 caattaatat taatgcaaat aaaaatatcc ggcaggtatg ctaaaaggat aaatggcgtt
     6601 ctctgtattt attcgcaatt atctatagga caaatatgga gagcttgatg agtcagagct
     6661 aaaacagcaa ttgataaata aatatgttga tttattcaag aaaatggatg tattttaaat
     6721 attgattctt aatataacct aatcaataga ttatcacgtg agagctgcac attattggcg
     6781 gggccgggga aaacatcatt ttgggaacaa tgaatcaggt actcttacca ctgaagcaag
     6841 atcatccggg caccaggtgg tggtgcagtg ctttggaaat ggtcctctcg gggggagttg
     6901 ccattatttt ctatgctatt tgcttattat attcatcagg tgaatttaat aaattcctaa
     6961 tttgaaatat ccactattaa gccagtgttt aacgacgtta agatggcaat gtgcagatca
     7021 tgttatcagg gtgggcggta gcgtatattc cttatacaaa ccatcattca tttcaatgat
     7081 tgttattgaa ttatttattg ttgtgaataa aaagacatta tgaatattgg tttgactttg
     7141 catatcaggt tttttattac tcgctgtaat gtacgagcct gtcgtgaata acgtcgagaa
     7201 cccatccgtt gcgtttgtga ttattttgtt gactaaacag acacccgttt ctctgaagta
     7261 aaatcccaga ctaaatcatc acataaccat gacatttttc tgatattccc cggtaacgcc
     7321 agatgtcgac tcgcttatct catccgcccg acatgttgta catgcaatat gtgcggatta
     7381 ttattcattt tcacggaggt tgctatgtca accggattac gtttcacact ggaagtggac
     7441 ggcctgccac cggatgcttt tgcggtggtt tcctttcatt tgaaccagtc actctcttcg
     7501 ctcttttccc tcgatctctc tctggtcagc cagcagtttc tctcccttga atttgcgcag
     7561 gtgctggaca aaatggccta cctgacggta tggcagggcg atgacgtaca gcgccgggtg
     7621 aaaggtgtgg tgacctggtt tgaactgggg gagaacgaca aaaaccagat gctgtacagc
     7681 atgaaggtat gcccaccgct gtggcgcata gggctgcgcc agaacttccg tatcttccag
     7741 aatgaggaca tcgaaagcat cctcgcgacg atcctgaagg aaaacggtgt gaccgagtgg
     7801 agtccgctgt tcagcgagcc gcatccttcc cgtgagtttt gtgtccagta cggcgaaact
     7861 gattacgatt tcctgtgccg gatggcggcg gaggaaggca tcttctttta tgaggagcac
     7921 gcgcaaaaaa gtaccgacca gagcctggtc ctgtgcgaca ccgtgcgtta tctgccggag
     7981 tcctttgaga tcccctggaa cccgaacacc cgtaccgagg taagtaccct ctgcatcagc
     8041 cagtttcgct acagcgcaca aatccgtcct tcttccgtag tgaccaaaga ctacaccttt
     8101 aaacgtcccg gctggccagg gcgttttgat caggaaggcc agtaccagga ttaccagcgc
     8161 acgcagtatg aagtgtatga ctaccccgga cgtttcaagg gtgcccacgg gcagaacttt
     8221 gcccgctggc agatggacgg ctggcgaaac aacgcagaag tggcgcgcgg aacaagccgt
     8281 tcgccggaga tatggccggg acggcgaatt gtgctgacgg ggcatccgca ggcgaacctg
     8341 aaccgggaat ggcaggtggt ggcgagcgat cttcacggcg aacagccgca ggcggtaccg
     8401 ggacgcaggg gttcaggcac cacgctggag aaccattttg cggtaatccc ggcagacaga
     8461 acatggcggc cacagccgtt gatgaaaccg ctggtggatg gcccgcagag cgccgtcgtg
     8521 acgggaccgg caggcgagga aatcttctgt gatgaacatg gccgcgtgcg ggtgaaattt
     8581 aactgggacc gctataaccc gtcaaaccag gacagttcat gctggatccg tgtggcacag
     8641 gcgtgggcag gcaccggctt tggcaacctg gcgataccgc gtgtgggtca ggaggtgatt
     8701 gtggacttcc tcaacggcga cccggaccag ccgattatta tggggcgtac ctaccaccag
     8761 gaaaaccgca cacccggcag cctgccgggg acgaagacgc agatgaccat tcgttcgaaa
     8821 acctataagg gcagcgggtt taatgaactg aagtttgacg atgcgacagg gaaagaacag
     8881 gtctacatcc acgcgcagaa gaacatgaac accgaggtgc tgaataaccg caccacggat
     8941 gtgataaaca accatgctga aaccattggc aacaatcaga tgattgcggt taccaacaat
     9001 cagatagaga cggtgggcgt taaccagata gagacggtgg gcagtaacca gataattaaa
     9061 gtgggttctg ttcaggtgga aacgattgga cttgttcgtg cgctgacggt gggcgtggcg
     9121 tatcagacga cggtaggtgg cattatgaac acctcggtgg cactgatgca gtcctcgcag
     9181 atcggtttgc ataaatcgtt gagggtcggg ctgggttatg acgtcaaagt cggaaataac
     9241 gttaccttca ccgttggtaa aacgaaaaag gatgataccg ggcagaccgc gatttactcc
     9301 gccggtgagc atctggagct ctgctgtggt aaggcaaggc tggtgctgac gaaggacgga
     9361 caaatttttc tcaacggcac aaaaattcat ttgcagggta aggagcaggt taatggtgac
     9421 tcactgttga ttaactggaa ctgtgcagcc tcgaaatccc caccgaagac ccctgatgaa
     9481 aagcaggata cgccggatat gagagagtac tgatcccgta cctgttcggt attcagtaac
     9541 aaaccagcat taaaaataaa cagaagaagg aatttgaatc atgagcggaa aaccagcggc
     9601 gcgtcaggga gatatgactc agtatggcgg tcccattgtc cagggttcgg caggtgtaag
     9661 aattggcgcg cccaccggcg tggcgtgctc ggtgtgtccg ggcgggatga cttcgggcaa
     9721 cccggtaaat ccgctgctgg gggcgaaggt gctgcccggc gagacggacc ttgcgctgcc
     9781 cggcccgctg ccgttcattc tctcccgcac ctacagcagc taccggacga agacgcctgc
     9841 accggtgggc gttttcggcc ccggctggaa agcgccttct gatatccgct tacagctacg
     9901 tgatgacgga ctgatactca acgacaacgg cgggcggagc attcactttg agccgctgct
     9961 gccgggggag gcggtgtaca gccgcagtga gtcaatgtgg ctggtgcgcg gtggtaaggc
    10021 agcacagccg gacggccata cgctggcgcg gctgtggggg gcgctgcgcc ggatatccgg
    10081 ttaagcccgc atctttacct ggcgaccaac agcgcacagg ggccgtggtg gatactgggg
    10141 ggggctgagc gggtgccggg tgctgaggac gtactgccag cgccgctgcc gccgtaccgg
    10201 gtgcttaccg ggatggcgga cgctgacgta ccggcgtgag gccgccggtg acctggccgg
    10261 ggaaatcacc gacgtgacgg acggtgccgg gcgggagttc cgtctggtgc tgaccacgca
    10321 ggcgcagcgt gcggaagagg cccgcacctc ttcgctatct tcttctgaca gttcccgccc
    10381 tctctcagcc tcagcgttcc ccgacacact gcctggtacc gaatacggcc ccgacagggg
    10441 tatccgcctt tcggcggtgt ggttgatgca cgacccggca tacccggaga gcctgcccgc
    10501 tgcgccactg gtgcggtaca cgtatacgga agccggtgaa ctgctggcgg tatatgaccg
    10561 cagcaatacg caggtgcgcg ctttcacgta tgacgcgcag cacccgggcc ggatggtggc
    10621 gcaccgttac gcgggaaggc cggagatgcg ctaccgctac gacgatacgg ggcgggtggt
    10681 ggagcaactg aacccggcag ggttaagcta ccgctatctt tatgagcagg accgcatcac
    10741 cgtcaccgac agcctgaacc ggcgtgaggt gctgcataca gaaggcgggg ccgggctgaa
    10801 acgggtggtg aaaaaagaac tggcggacgg cagcgtcacg cgcagcgggt atgacgcggc
    10861 aggaaggctc acggcgcaga cggacgcggc gggacggcgg acagagtacg gtctgaatgt
    10921 ggtgtccggc gatatcacgg acatcaccac accggacggg cgggagacga aattttacta
    10981 taacgacggg aaccagctga cggcggtggt gtacccggac gggctggaga gccgccggga
    11041 atatgatgaa ccgggcaggc tggtatcgga gacatcgcgc agcggggaga cagtacgcta
    11101 ccgctacgat gacgcgcaca gtgagttacc ggcgacgaca acggatgcga cgggcagcac
    11161 ccggcagatg acgtggagcc gctacgggca gttgctggcg ttcactgact gctcgggcta
    11221 ccagacccgc tatgaatacg accgcttcgg ccagatgacg gcggttcacc gcgaggaagg
    11281 tatcagcctt taccgccact atgacaaccg tggccggtta acctcggtga aagacgcaca
    11341 gggccgtgaa acgcagtatg agtacaacgc cgcaggcgac ctgactgccg ttatcacccc
    11401 ggacggcaac cggagcgaga cacagtacga tgcgtgggga aaggcggtca gcaccacgca
    11461 gggcgggctg acgcgcagta tggagtacga tgctgccgga cgtgtcatca gtctgactaa
    11521 cgagaacggc agtcacagcg acttcagcta cgatgcgctg gaccggctgg tacagcaggg
    11581 cggctttgac gggcggacgc aacgttatca ttatgacctg accggaaaac tcacgcagag
    11641 tgaggatgag gggcttgtca ccctctggca ctacgatgcg tcggaccgca tcacgcaccg
    11701 gacggtgaac ggcgacccgg cagagcagtg gcagtatgac ggccacggct ggctgagaga
    11761 aatcagccac ctgagcgaag gccaccgtgt cgctgtccac tacggctacg acgataaggg
    11821 ccgcctgacg ggcgaacggc agacggtgga gaacccggag acgggggaac tgctgtggca
    11881 gcatgagacg aaacacgcct acaacgaaca ggggctggca aaccgcgtca cgccggacag
    11941 cctgccgccg gtggagtggc tgacctacgg cagcggttac ctggcgggca tgaagctggg
    12001 cgggacgccg ctggtcgagt atacgcggga caggctgcac cgtgagacgg tgcgcaccct
    12061 cgcaccggac ctggacatcc gcatcccgta tgccaccgac ccggcgggaa accgcctgcc
    12121 ggacccggag ctacacccgg acagcacgct cacggcgtgg ccggataacc gcatcgcaga
    12181 ggatgcgcac tatgtttacc actatgatga atacggcaga ctgacggaga agacggaccg
    12241 catcccgacg ggtgtgatac ggacggacga cgagcggacc caccactacg acagccagca
    12301 ccgcctggtg ttccacacgc ggatacagca tggcgagcca ctggtcgaga gccgctacct
    12361 ctacgacccg ctgggacggc gaatggcgaa acgggtatgg cggcgggagc gtgacctgac
    12421 ggggtggatg tcgctgtcgc gtaaaccgga ggagacatgg tacggctggg acggcgacag
    12481 gctgacgacg gtacagaccg acaccacacg tatccagacg gtatacgagc cgggaagctt
    12541 cacgccgctc atccgggtcg agacagagaa cggcgagcgg gagaaagcgc agcgccgcag
    12601 cctggcggag aagctgcagc aggaagggag cgaggacggg cacggcgtgg tatttccggc
    12661 tgaactggtg cggctgctgg acagactgga ggaagaaatc cgggcagacc gcgtgagcag
    12721 tgaaagccgg gtgtggcttg cgcagtgcgg gctgacggtg gagcaactgg ccagacaggt
    12781 ggagccggaa tacacaccag tgcgaaacgt tcatctttac cactgcgacc atcggggcca
    12841 gccgctggcg ctcatcagcg aagacggcga tacggtgtgg agcggggcgt atgatgagtg
    12901 gggcaaccag ctaaatgagg agaacccgca tcatgtgtat cagccatacc gtctgccggg
    12961 gcagcagtat gatgaggagt cggggctgta ctacaatcgt caccggtact atgatccgtt
    13021 gcagagacgg tacatcaccc aggaccccat tgggctgaaa ggtgggtgga atctgtatca
    13081 gtatccgctg aatccggtga gcaggataga tccgttgggg ttattctcat gtgagagttg
    13141 tagtcatttt atagatgata taaataataa actggataac cttccgttta acccgacaaa
    13201 aatgtttaca gctagtttaa atactgctaa ttcagcaagg ctgtatgcaa gtggcatgac
    13261 aaaattcggt ataagtgttg ctgccgatgg aacgatcatt gctgccccag tcggtgcagc
    13321 ttctatggct ttaggggcgt ggaatataaa atctgcgaca gctgcatata acagagcaaa
    13381 tttacaatta tcagaatcac ttcaggaaaa tggtagagat aggtgttgga gaaatttatt
    13441 gggtatttta ccttatggtg agcagtttga tgaccctggt gaaccatcaa tattagacgt
    13501 ttatggtgaa aagattgaga atatcaaaga ttctccaatg aagttttttg aagagcttgg
    13561 gacgctgggg ttgtaatgtg tatttttaat gatattttta tgtttgtgtt tattgttagt
    13621 agtatacttg taaccgtgca ttttgtgtat tctttatttt ttaaaagtgg aaaggtaaaa
    13681 gataaggtaa acaaatggat taaagtaatg tgtgatttat tttgggggat aggatagtgg
    13741 tgtgatatgg ataaatataa attacagtgg ctggcatatt tcgacctgac gccggagtag
    13801 ataaaaaaac agacaggatt ggagtatagc aagcgtctga caatctctcg ggacagtagt
    13861 atgatgaaga ggcggggttg tactataacc gtaaccggta ctacgatccg ctaaagggca
    13921 agtatatgac tcagggaaag atcatcagaa gttcactttt tgtactaaat aattcgcatt
    13981 ttatgtttaa aaattgagat attcctcatt acctgaagct gtttttttat tgcttataca
    14041 taatcaaata ctccttacat aattaaggag aacaaaatgg aaattaaaaa attgatggaa
    14101 cattttttga agcaatatgg tgattttgaa aatggcattc ctgttcacga taccattgcc
    14161 agagttgtat cctgtatcag tcctgcaaaa tttcatgagt gctttattaa ctggatgcgt
    14221 gactgccatt cttcagatga taaagacgtc attgcaattg atggaaaaac gctccggcac
    14281 tcttatgata agagtcgtcg caggggagcg attcatgtca ttagtgcgtt ctcaacaatg
    14341 tacagtctgg tcctcgggca gatcaagacg gatgagaaat ccaatgagat tacagccatt
    14401 cctgaacttc ttaacatgct ggatattaaa ggaaaaatca tcacaactga tgcgatgggt
    14461 tgccagaaag atattgcaga gaagatacaa aaacagggag gtgattattt attcgctgta
    14521 aaaggaaacc aggggcggct aaataaagcc tttgaggaaa aatttccgct gaaagaatta
    14581 aataatccag agcatgacag ttacgcaatc agtgaaaaga gtcacggcag agaagaaatc
    14641 cgtcttcata ttgtttgcga tgtccctgat gaacttattg atttcacgtt tgaatggaaa
    14701 ggactgaaga aattatgcgt ggcagtctcc tttcggtcaa taatagcaga acaaaagaaa
    14761 gagccagaaa tgacggtcag atattatatc agttctgctg atttaactgc agaaaagttc
    14821 gccacagcaa tccgaaacca ctggcacgtg gagaataagc tgcactggcg tctggacgtg
    14881 gtaatgaatg aagacgactg caaaataaga agaggaaatg cagcagaatt attttcaggg
    14941 atacggcaca ttactattaa tattttgacg aatgataagg tattcaaggc agggttaaga
    15001 cgtaagatgc gaaaagcagc catggacaga aactatctgg cgtcagtcct tgcggggagc
    15061 gggctttcgt aatcttgccc tgctgaagag tttgaaaggg cacggatcct ttcaaaagaa
    15121 tacattgaga aatatataag ctaaagatga ttagctgtat ttcatgataa taatctaatg
    15181 agggattttt ctgaacatca aattgcatcg aagttagtta gagtgctgcg attgtcttgc
    15241 acgtaataaa ttcagagaga aaatatagtt gtgggtatga gaaaataacc aacctaatgt
    15301 aaatcaattc agatgaagag gaataaacca tg
//
LOCUS       DAQSWQ010000101        15180 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.102, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000101 DAQSWQ010000000
VERSION     DAQSWQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.102"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..775
                     /locus_tag="R8G18_003260"
                     /pseudo
     CDS             1..775
                     /locus_tag="R8G18_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            1098..2486
                     /gene="narU"
                     /locus_tag="R8G18_003261"
     CDS             1098..2486
                     /gene="narU"
                     /locus_tag="R8G18_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HEE9796879.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2568..6308
                     /gene="narZ"
                     /locus_tag="R8G18_003262"
     CDS             2568..6308
                     /gene="narZ"
                     /locus_tag="R8G18_003262"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HEE9796880.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6305..7849
                     /gene="narH"
                     /locus_tag="R8G18_003263"
     CDS             6305..7849
                     /gene="narH"
                     /locus_tag="R8G18_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEE9796881.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7849..8544
                     /gene="narW"
                     /locus_tag="R8G18_003264"
     CDS             7849..8544
                     /gene="narW"
                     /locus_tag="R8G18_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEE9796882.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8541..9221
                     /gene="narI"
                     /locus_tag="R8G18_003265"
     CDS             8541..9221
                     /gene="narI"
                     /locus_tag="R8G18_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEE9796883.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9300..10193
                     /locus_tag="R8G18_003266"
     CDS             9300..10193
                     /locus_tag="R8G18_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HEE9796884.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(10289..11134)
                     /gene="nhoA"
                     /locus_tag="R8G18_003267"
     CDS             complement(10289..11134)
                     /gene="nhoA"
                     /locus_tag="R8G18_003267"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HEE9796885.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11307..11876
                     /locus_tag="R8G18_003268"
     CDS             11307..11876
                     /locus_tag="R8G18_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEE9796886.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11880..12107)
                     /gene="pptA"
                     /locus_tag="R8G18_003269"
     CDS             complement(11880..12107)
                     /gene="pptA"
                     /locus_tag="R8G18_003269"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HEE9796887.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(12207..13342)
                     /locus_tag="R8G18_003270"
                     /pseudo
     CDS             complement(12207..13342)
                     /locus_tag="R8G18_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(13482..14618)
                     /locus_tag="R8G18_003271"
     CDS             complement(13482..14618)
                     /locus_tag="R8G18_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEE9796888.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSTDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNP
                     AHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            complement(14975..15151)
                     /locus_tag="R8G18_003272"
     CDS             complement(14975..15151)
                     /locus_tag="R8G18_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796889.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT         3780 a         3783 c         3618 g         3999 t
ORIGIN      
        1 tttgtgcaat tagaatcttg cgaatgtctg caatacctga atctcagcca taatcaatta
       61 tctgatattg ttgcaggtaa taaaaattaa ctcttactgc tggatctatc ccataataaa
      121 ctaacaagtt tacacaatgt cttatttccc aacttgaata cgttacttat taacaaaaac
      181 ctgctttctg aaattaaaat attctatagc aacttctgca atgttcagac attaaacgct
      241 gctaacaacc agttgaaaaa tataaatctt gatttcctga cttgtcttcc atctattaaa
      301 agtttaagac tggacaacaa taaaataacc catattgata ctaataatac atccgatatt
      361 ggaactttat ttcccataat aaaacagagc aaaaacttaa attttttaaa tgtttctggg
      421 aagaacaatt gccctactat gcagctcatg ttatttaatt tgttttcccc aacacttaag
      481 cttaatactg gcctggcaat tctttcgcct ggtgcatttg aagttcactc tgacggaata
      541 gatgtggata acgaattgtt tcactatcct attaaaaata gcatataccc catataatat
      601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
      661 cttagatgaa ataaacaata cttactgtaa taacgattat tacaatcagg caataagaga
      721 ggaaccgata gaccttctgg acagatcgtt ttcctccagt tcatggcctt tttagtgaat
      781 aaatcatcac taattctata agtgtctggt cttgttgata atatagttaa tatgtaaccc
      841 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta accccgtttg
      901 aaggttagct tgttagttac atttagtaac acatatctgg ctacttaatc cccattctgc
      961 ccccgcggtt acgctttctt atctattctt tcaatcagca cttcacccaa ttcatcctta
     1021 attctaataa ctggctattg cgccttacct cctgttttgt cttttttcat taccaatgtg
     1081 tgcatgtgag gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat cttttacgcg
     1141 actggaaacc agaaaatccg gccttctggg aaaataaagg aaaacatatt gctcgaagaa
     1201 acctctggat atcagtcagt tgtctacttc ttgccttctg tgtctggatg ctatttagcg
     1261 cagttaccgt taatctcaat aaaatcggtt ttaattttac taccgatcaa ctctttttat
     1321 taaccgcatt accctccgtt tctggcgcat tattgcgtgt tccctactcc tttatggtgc
     1381 ctatattcgg tggacgccga tggacggttt ttagtactgc aatcctgatt attccttgcg
     1441 tctggctcgg aattgccgtg caaaatccga atactccttt tgggatattt atcgttatcg
     1501 ctttgctatg cggttttgca ggtgcaaact ttgcttcgag catgggcaat atcagtttct
     1561 tctttccaaa agccaaacaa gggagcgctc ttgggattaa tggcggatta ggaaacttag
     1621 gtgtaagtgt aatgcagctg gttgcaccgc tggtcatttt tgtacctgta tttgcctttc
     1681 tcggcgtcaa tggcgtaccg caggccgacg gttcggtgat gtcgctggcg aatgccgcat
     1741 ggatttgggt accgctactg gcgattgcca cgatcgccgc ctggtcaggg atgaatgata
     1801 tcgccagttc acgcgcctca attgacgacc agctccctgt cttacaacgc ctgcatctct
     1861 ggctgctgag cctgctttac cttgccacct tcggttcgtt tatcggtttt tctgcgggtt
     1921 ttgccatgct ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg gcgttctttg
     1981 gcccatttat cggtgccatc gcgcggtcgg ttggtggtgc tatttccgat aaattcggcg
     2041 gcgtgcgggt gacgttgatc aactttattt ttatggcgat tttcagtgcc ctgctgttcc
     2101 ttaccttacc gggcacaggc tccggtaatt tcatcgcctt ttacgccgta tttatggggc
     2161 tgtttctgac tgcgggtctg ggaagtggtt ctactttcca gatgatcgcc gtcatctttc
     2221 gccagataac catttatcgg gtgaagatga aaggcggtag tgatgagcaa gctcagaaag
     2281 aagccatcac cgaaacggcg gcggctctgg gctttatctc agccattggc gcagtgggcg
     2341 gcttttttat tccgcaggcg tttggcatgt cgctcaatat gaccggctct ccggtcggcg
     2401 cgatgaaagt gtttttaatc ttctacatcg tttgtgtgct gctgacctgg ctggtttatg
     2461 gtcggcggaa gttcagccaa aaataagaga tcgatgcctg atgtacaacc gtgtcgcatc
     2521 cgacaaggca tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt
     2581 tggatcgctt tcgctacttc aaacaaaagg gcgaaacctt tgccgatggt cacggacagg
     2641 tgatgcatag caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa
     2701 tcgtgcgttc cacccacggt gttaactgta caggctcctg tagctggaaa atctacgtta
     2761 aaaatggtct ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc
     2821 agcccaatca tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctctaca
     2881 gcgctaaccg cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgtgaag
     2941 ctctcaagca acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa
     3001 agtgcttaag ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg
     3061 aacttaacca actgattgcc gccgctaacg tctggaccat caaaatctat ggtccggatc
     3121 gcgttgccgg tttctcgccg atcccggcga tgtcgatggt gtcatacgct gccggaacgc
     3181 gttatctgtc gctgttaggc ggcacctgtt taagcttcta cgactggtat tgcgacctgc
     3241 cgcccgcctc gccgatgacc tggggcgaac aaaccgacgt gccggaatct gccgactggt
     3301 ataactccag ttacatcatc gcctgggggt ctaacgtacc gcagacacgt acgccggacg
     3361 cccacttctt taccgaagta cgttacaaag gcaccaaaac tatcgccatt acccctgact
     3421 actctgaagt ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagcg
     3481 ccctggcgat ggcaatgggc catgtgattt taaaagagtt tcatctcgat aatcccagcg
     3541 actactttat caactactgc cgccgctata gcgacatgcc gatgctggta atgctggaac
     3601 ctcgcgacga tggtagctac gtacccgggc gcatgatccg cgcgtctgac ctggtagatg
     3661 gactaggcga aagcaataat ccgcagtgga aaaccgtggc ggttaatacc gcaggtgaat
     3721 tggtagtgcc gaatggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg
     3781 aatccattgc cgccggtaag gaaaccgaat tgtcgttaac cctgctcggt caacatgacg
     3841 ctgttgcagg cgtggccttc ccctactttg gcggcattga aaatccgcat tttcgcagcg
     3901 taaaacacaa tccggtgctg gtgcgccaat tgcccgttaa aaacctgacg ttagtcgatg
     3961 gcaacacctg tccggtggtc agcgtttatg atttggtgct ggcgaattac ggcctcgatc
     4021 gcgggctgga agatgaaaac agcgcgaaag attacgctga aatcaaaccg tacaccccag
     4081 cctggggtga gcaaattacc ggcgtaccgc gccagtatat tgaaaccatc gcccgcgaat
     4141 ttgccgatac cgcccataaa acgcatggac gctcaatgat tatcctcggc gctggcgtca
     4201 accactggta tcacatggat atgaactacc gtgggatgat caatatgctg atcttctgcg
     4261 gttgtgtcgg gcaaagcggc ggcggctggg cgcactatgt cggtcaggaa aaactgcgcc
     4321 cacaaaccgg ctggttgccg ctggcctttg cgctcgactg gaaccgccca ccgcgccaga
     4381 tgaacagtac ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg
     4441 ctcaggagtt actttcaccg cttgccgatg ccagtaagta cagcggtcat ctgattgatt
     4501 tcaacgttcg cgccgaacgc atgggctggc taccttctgc gccgcagctg gggcgtaacc
     4561 cgctcgggat taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc
     4621 aggcgctgaa atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc
     4681 atccgcgtaa tttgtttgtc tggcgctcta acctgcttgg ctcctccggc aaaggccacg
     4741 agtatatgca gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg
     4801 ccagcgacgg gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc
     4861 tcgacctgct ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg
     4921 ttctgcccac cgccacctgg tacgaaaaag acgatatgaa cacctcggat atgcatccgt
     4981 ttattcatcc gctttctgcg gcggtcgatc cggcgtggga atcacgcagc gactgggaaa
     5041 tctacaaagg tatcgccaaa gcattttcgc aagtgtgcgt gggtcatctt ggcaaagaaa
     5101 ccgacgtggt attacaaccc ctactgcatg actctccggc agagctctca cagccgtgtg
     5161 aagtgctcga ctggcgcaaa ggcgaatgcg atctgattcc aggcaaaacc gcgccgaata
     5221 ttgtggcggt ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat
     5281 tgatggacaa acttggcaat ggtggtaaag ggatttcgtg gaatacccag gatgaaatcg
     5341 atttcctcgg caaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga
     5401 ttgacaccgc cattgacgct tcagaagtga ttctggcact ggcaccggaa accaacggtc
     5461 atgttgcagt caaagcgtgg caggcgctgg gcgagatcac cgggcgcgaa catacccatc
     5521 tggcgctgca caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta
     5581 aaattatctc cagccccaca tggtccggtc tggaaagcga tcacgtctcc tataatgcgg
     5641 gatacaccaa cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct
     5701 atcaggatca tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta
     5761 tcgacacccg tagcgtcagt gagatgcgcc agatcccgcc aaacggcttc ccggaaaaag
     5821 cacttaactt cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc
     5881 tgctaatgct gacgctctct cgcggtggac cgattgtctg gatcagcgaa accgatgccc
     5941 gagaactgac cattgtcgat aacgactggg tggaagtctt taacgccaac ggcgcgctga
     6001 cggctcgtgc ggtggtcagc caacgtgtac cgccgggtat gaccatgatg tatcacgccc
     6061 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     6121 actcggtcac ccgtgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     6181 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     6241 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     6301 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgacaaat gtatcggttg
     6361 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     6421 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     6481 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatacgcc cgcgtctggg
     6541 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agattgatga
     6601 ttactacgaa cctttcacct tcgactacga acatttgcat agcgcaccgg aaggcaaaca
     6661 tatccctact gctcgcccgc gttcgctgat tgacggtaag cggatggaca aagtgatctg
     6721 ggggccaaac tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa
     6781 cttcgaggcc atgcaaaagg agatgtacgg gcagtttaaa aacaccttca tgatgtacct
     6841 gccgcgcctg tgcgaacact gcctcaatcc cagttgcgtg gcgacctgcc caagcggcgc
     6901 tatctacaaa cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg
     6961 gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa
     7021 gtcagaaaaa tgtatcttct gttatccgcg aattgagtca ggccaaccca ccgtgtgctc
     7081 agaaacctgc gtgggtcgca tccggtatct gggcgtgctg ctttacgacg ccgaccgcat
     7141 tgaggaagcg gcgagcaccg agcgcgaagt tgacctctat gaacgtcagt gcgaagtgtt
     7201 cctcgatcca cacgatccct cagtgatcga ggaagccctg aaacaaggta ttccacaaaa
     7261 cgtgattgaa gctgcccagc gttcgcctgt ctacaaaatg gcgatggact ggaaactggc
     7321 gctaccgctg caccctgaat atcgcaccct gccaatggtc tggtacgttc ctccgctgtc
     7381 gccgattcag tcctacgcag atgcgggcgg tttgccgaaa agcgaaggcg tgctgcccgc
     7441 catcgaaagc ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggcgatac
     7501 cggtccggta ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca
     7561 aaccgtggaa ggcgttactg atactcgtgc catcgacgaa gtaggcctga gcgtcgccca
     7621 ggtcgaagaa atgtatcgct atctcgccat tgccaactat gaagatcgtt ttgttatccc
     7681 gaccagccat cgggaaatgg cgggcgatgc cttcgcagaa cgcaacggct gcggttttac
     7741 ctttggcgac ggttgccacg gttcggacag taaattcaac ctgttcaaca gtagccgtat
     7801 cgatgccatc aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc
     7861 aaagtgatcg gcctgttgat ggagtatccg gatgagctgt tgtgggaatg caaggaggac
     7921 gcgctggcgt tgatccgccg cgacgcgccg atgcttacgg atttcaccca caacctgctt
     7981 aacgcgccgc tgctggataa acaggccgaa tggtgcgaag tgtttgaccg cgggcgcacc
     8041 acgtcgctgc tgctgttcga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg
     8101 gtggacctgc tggcggagta tgaaaaggtc ggcctgcagc tggattgtcg ggaactgccc
     8161 gattatctac cgctgtatct ggagtattta agcgtactgc ctgacgatca ggcgaaagaa
     8221 ggattgctca atgttgcgcc gatcctcgcc ctgcttggcg gtcgcttaaa acaacgcgag
     8281 gcaccgtggt acgcgttgtt tgatgctctg ctgcaactgg cgggaagtat actttcaagt
     8341 gacagtgtca caaaacaagt aaacagcgaa gagcgcgatg acacccgcca ggcgcttgat
     8401 gcagtgtggg aagaggaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc
     8461 tcgccgctta atcaatatca gcgacgcttt agccaggatg tcgcgccgca gtatgtcgac
     8521 atcagtgcgg gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc
     8581 cgtacatttg tgcgacggtg tttttcctcg gcagctggct gcgctacgac tacgggcagt
     8641 acacctggcg cgcctcctca agtcaaatgc tcgataaacg cgggatggtg atatggtcga
     8701 atctgttcca tatcggcatt ttggggattt tcttcgggca cttgttcggc atgttaacgc
     8761 cgcactggat gtacgcgtgg tttttgccag tggcagcgaa acaactcatg gcgatggtgc
     8821 tcggcggtat ttgcggtgtg ttgacgttga ttggcggcgc aggactgctg tggcgcagtc
     8881 tgacaaatca gcgcgtgcgt gccacttcca ctacgccgga tatcatcatc atgagcattt
     8941 tgctgatcca gtgtctgttg gggctaagta ccataccgtt ttcagcacag tatcctgatg
     9001 gtagcgaaat gatgaagctg gtgggttggg cgcaaagcat tgtcactttc cgtggtggat
     9061 cgtcagagat gctcaacggc gtagcgttcg tcttccgcct gcatctggtg ttgggaatga
     9121 ccatcttcct gctcttcccg ttcacccgac tggtgcatgt gtggagcgcg ccgtttgagt
     9181 actttactcg tcgatatcaa attgtgcgtt cgcggcgata atttcaccct ggccggatag
     9241 cccttcgctc tccggccact tgcaggctat gatagttctc cagtcacagg agaacgttaa
     9301 tgaaaccgca ggtgtaccac gtcgatgctt ttacctctca accatttcgc ggcaattctg
     9361 ccggtgttgt tttccccgcc gataatctta gcgaagcaca aatgcagctt atcgcccgcg
     9421 agttaggcca ttcggaaact gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc
     9481 gctactttac gccaacggtt gaagtgccga tttgcggtca cgcgacagta gctgcgcact
     9541 atgtacgtgc gaaggtgtta ggtttaggaa attgcacgat ctggcaaaca tcgctggcag
     9601 gaaaacatcg cgtgactatc gaaaagcaca acgatgatta tcgtatttcg ctggaacaag
     9661 gtacgccggg ctttgaactg ccgctggagg gtgaagtacg cgcggcgatt atcaacgcat
     9721 tgcatcttac tgaagacgat attctgccgg gtttgccgat ccaggtcgcg acgaccgggc
     9781 attctaaagt gatgatccca ttgaagccgg aagtggatat cgacgctctt tcgcctgacc
     9841 ttaatgcgct gaccgctatc agtaaacaga ttggctgcaa tggtttcttc ccgttccaga
     9901 tccgtccagg caaaaacgaa accgatggtc gtatgttctc gcctgcgatt ggtattgtgg
     9961 aagatccggt gactggcaat gccaatggcc cgatgggtgc atggttggta catcacaacg
    10021 tattgcccca cgatggcaac gtgttgtgtg ttaaaggcca tcaggggcgc gcattggggc
    10081 gtgacggcat gattgaagtg acggtgacaa ttcgtgataa ccaaccggaa aaagtcacca
    10141 tttcaggcac agcggtgatt ttgtttcatg ccgaatgggc aattgaaatc tgaaatcgtt
    10201 gcctgatgcg cttcgcctgt caggtctaca accgggctaa tgtatgccgg ataaggcgtt
    10261 tacgccgcat ccggcaacct gacataaatt attttcccgc ctccggatgc gtatcaaacg
    10321 ccgccatcac cagcgctaac tcatccacag taaagccatg tttcgcatca tccacgccca
    10381 gaccaaactg ttcctgcatc acggcatata acgacgccac atccggtaga tttcgctgct
    10441 ccaccgcgtg cccattttca taatgggtaa aatgaaaatt ggtcagtgtc agcttgccgc
    10501 cgtccggcaa atggcggcac atcagcaaat gatggcgaaa atgcgactgc ggccagtgcg
    10561 ccgaccagaa attgcccatc acataatcgc tttgttgctg ctcgcagaga tcgaagcggt
    10621 acatcggctg ccaatgctga tgatgattaa actgcaacac ccaatcatca ccctcctgca
    10681 acaaccgata ctctccgtgt ggcgtggtct gcacgagatc ggaaactaaa cgaatcggcg
    10741 cggttagcgt ctgcccaccg aaaccgacat cagcaatcca tttttcccct tccagttcca
    10801 ccaacagcaa acgatgggtg cgcggcggta atgctggcgg atttgataac actacgcgcc
    10861 ctaacaagct gcgaacgtta aaccccagct cgcgtaacac ccgctcaaac acgccattct
    10921 gctcaaaaca gtaaccgcca cgacgggcta tcaccagttt ctcttccagc gattgatcat
    10981 caagctgtat ttccctcggc agcaaaacgt caaggttttc aaacggaatg gtgcaattgt
    11041 gtttcaggtg caatgcacga agcgtatcaa tattgaccgc agcagctccc gaccagttaa
    11101 tacgggcaaa atagtgattc agaatgggcg tcatcaggcg tttcccttag tggttttctc
    11161 aacttataac ctggaataga cgccgcaccg gctattttgt gcgatcgcgg acatgcatca
    11221 cgtttgcaaa tcgccaatcc cgttaatttc gttacttatt gcgttacgga ctacgcttaa
    11281 aaaaaccttc ttcgcgaggc gataccatga gccgattcat ccccatagaa ttacaccatg
    11341 ccagccgtct gttaaatcat ggcccaactg tcttgatcac cagtttcgat gagcaatccc
    11401 agcggcgtaa cattatggct gcagcctggt caatgccggt ggagtttgaa cctccacgcg
    11461 tggcaattgt ggtagataaa tcgacatgga ccagggagtt gattgaacgt aacggtaagt
    11521 ttggcatcgt tatcccgggc gttgcagcaa ctaactggac gtgggcggtg ggaagtgtgt
    11581 cggtgcgtga agaagataaa tttaattgct atggcattcc ggtggtgaaa ggtccggtgc
    11641 ttggtttgcc acttgttgaa gagaaatgcc tggcgtggat ggagtgccgc ctgctacctg
    11701 cgacttctgc gcaacaaaaa tacgacacgc tgtttggcga ggtggtatca gcagcggcag
    11761 acgcacgggt atttgtcgaa ggccgctggc agtttgatga tgataagctc aatacgttgc
    11821 atcatttagg tgctggcacg tttgttacta gcggcaagcg tgttacggca ggttaagcat
    11881 tacatgctgt aaccgggttt ctttatcaaa gcctccattt ggggctcgat ttcggcatcc
    11941 cagatagctt gccaagattc tggttgaatc tgctgtagag caatgcttat cgagctatct
    12001 ttactgttca gatggcgaat aataacgtcg gtaatatctg cagcaagtgc tgctttttgt
    12061 tgttcgtcca gttcacgcgg aaagcattta atgtcgatgt gcggcatgat tacgctcctt
    12121 ttgaaagatg tcagtaaaat taacacacat tgtgaatgtc atgtgtccgg taaagcgttt
    12181 atacgacaat aatgcccagg gcaagattac gaaagcccgc tccccgtaag gactgacgcc
    12241 aggtagtttc tgcccatggc tgcttttcgc atcttacgtc ttaaccctgc cttgaatacc
    12301 ttatcattcg tcaaaatatt aatagcaatg tgccgtatcc ctgaaaataa ttctgctgca
    12361 tttcctcttc ttattttgca gtcgtcttca ttcattacca cgtccagacg ccaatgcagc
    12421 ttattctcca cgtgccagtg gtttcggatt gctgtggcga acttctctgc ggttaaatca
    12481 gcagaactga tataatatct gaccgtcatt tctggctctt tcttttgttc tgctattatg
    12541 gaccgaaagg agactgccac gcataatttc ttcagccctt tccattcaaa cgtgaaatca
    12601 ataagttcat cagggacatc gcaaacaata tgaagacgga tttcttctct gccgtgactc
    12661 ttttcactaa ttgcgtaact gtcattctct ggattattta attctttcag cggaaatttt
    12721 tcctcaaagg ctttatttag ccgcccctgg tttcctttta ccgcgaataa ataatcacct
    12781 ccctgttttt gtatcttctc tgcaatatct ttctggcaac ccatcgcatc agttgtgatg
    12841 atttttcctt taatatccag catgttaaga agttcaggaa tggctgtaat ctcattggat
    12901 ttctcatccg tcttaatctg cccgagaatc agactgtgca ttgttgagaa cgcactaatg
    12961 acatgaattg ctcccctgcg gcactcttgt cataagagtg ccggagcgtt tttccatcaa
    13021 ttgcaatgac gtctttatca tctgaagaat ggcagtcacg catccagtta ataaagcact
    13081 cgtgaaattt tgcaggactg atacaggata caactctggc gatggtatcg tgaacaggaa
    13141 taccattttc aaaatcacca tattgtttta aaaaatcgag atgtgtttcc ccaaaatcct
    13201 ctatatcttc ccagccttct gcaccagaaa taacggcaca aatagtcaac agtagaatat
    13261 ccgataattt atgttccact ttccaggctt gtttttaatc ggggataata gaaatatgtt
    13321 ccatcaattt tttaagttcc attttgttct ccttaattat gtaaggagta tttgatcatg
    13381 tataagcaat aaaaaacagc ttcaggtaat aaggaatatc tcaattttta aacataaaat
    13441 gcgaattatt tagtacaaaa agtgaactcc tcagggcaag attacgaaag tccgctcccc
    13501 gcaaggactg acgcgagata gtttctgtcc atggctgctt ttcgcatctt acgtcttaac
    13561 cctgccttga ataccttatc attcgtcaaa acattaatag caatgtgccg tatccctgaa
    13621 aataattctg ctgcatttcc tcttcttatt ttgcagtcgt cttcattcat taccacgtcc
    13681 agacgccagt gcagattatt ctccacgtgc cagtgatttc gaatcgctgt cgcaaacttc
    13741 tcagcggtta aatcagcaga actgatataa tatctgacca tcatttcggg ctctttcttt
    13801 tgctctgcta ttattgagcg aaaggagact gccacgcata attttttcag ccctttccat
    13861 tcaaacgtga aatcaataag ttcatcaggg acatcgcaaa caatatgaag acggatttct
    13921 tctctgccgt gactcttttc actcattgcg gaactgtcat gcgctggatt atttaattct
    13981 gtcagcggaa attttttctc aaaggcttta tttagccgcc cctggtttcc ttttaccgcg
    14041 aataaataat cacctccctg tttttgtatc ttctctgcaa tatctttctg gcaacccatc
    14101 gcatcagttg tgatgatttt tcctttaata tccagcatgt taagaagttc tgggatagct
    14161 gtaatctcat tagatttctc atccgtcttg atctgtccga tgaccagact gtgcattgtt
    14221 gagaacgcac taatgacatg aatcgctccc ctgcggcgac tcttgtcata agagtgccgg
    14281 agcgtttttc catcaattgc aatgacgtct ttatcatctg tagaatggca gtcacgcatc
    14341 cagttaataa agcactcgtg aaattttgca ggactgatac aggatacaac tctggcaatg
    14401 gtatcgtgaa caggaatgcc attttcaaaa tcaccatatt gcttcaaaaa atcgagatgt
    14461 gtttccccaa aatcctctat atcttcccaa ccctctgcac cagaaataac ggcacaaata
    14521 gtcaacagta gaatatccga taatttatgt tccactttcc aggcttgtct gtaatcgggg
    14581 ataatagaaa tatgttccat caatttttta agttccattt tgttctcctt aattatgtaa
    14641 gaagtatttg atcatgtata agcaataaaa aacagcttca ggtaataagg aatatctcaa
    14701 tttttaaaca taaaatgcga attatttagt acaaaaagtg aacttctgat gatctttccc
    14761 tggcctgcca ccatctcttc acaagcttat gcttattaca tgttacatgc aaaacgtccc
    14821 gttttgcatc tttgtgccta cggcacaccg tgatctttcc ctgatgaggg atatattaac
    14881 tattgaacat tagcgcgaat tatttagtgc aaaaaatcag catcacgtga tgcttttcct
    14941 gctattaata acctcctcta tttaactata tttttcattg atgcaattcg tagatttgaa
    15001 gaagttcaac tgccactttt ttatttttac tatcgcaatt tttataccat acccatgctt
    15061 ccgtattttt tactattttt aaatcatcca taataaaatc cccaccaaaa acagtaaaat
    15121 acaatgtttc ctcattaaaa ataaaactca ctgccccccg ttactttatt cgtacccctt
//
LOCUS       DAQSWQ010000102        15169 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.103, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000102 DAQSWQ010000000
VERSION     DAQSWQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.103"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="R8G18_003273"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="R8G18_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HEE9796890.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="R8G18_003274"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="R8G18_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HEE9796891.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="R8G18_003275"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="R8G18_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HEE9796892.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="R8G18_003276"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="R8G18_003276"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HEE9796893.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="R8G18_003277"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="R8G18_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HEE9796894.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="R8G18_003278"
     CDS             complement(8214..9107)
                     /locus_tag="R8G18_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HEE9796895.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="R8G18_003279"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="R8G18_003279"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEE9796896.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="R8G18_003280"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="R8G18_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HEE9796897.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="R8G18_003281"
     CDS             complement(10238..11470)
                     /locus_tag="R8G18_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HEE9796898.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="R8G18_003282"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="R8G18_003282"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEE9796899.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="R8G18_003283"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="R8G18_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HEE9796900.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15169)
                     /locus_tag="R8G18_003284"
     CDS             complement(14275..>15169)
                     /locus_tag="R8G18_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEE9796901.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3559 a         4283 c         3740 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaat
//
LOCUS       DAQSWQ010000103        15149 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.104, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000103 DAQSWQ010000000
VERSION     DAQSWQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.104"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..122
                     /locus_tag="R8G18_003285"
     CDS             <1..122
                     /locus_tag="R8G18_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9796902.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="R8G18_003286"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="R8G18_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HEE9796903.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..3180
                     /gene="safA"
                     /locus_tag="R8G18_003287"
     CDS             2983..3180
                     /gene="safA"
                     /locus_tag="R8G18_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HEE9796904.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3255..4016
                     /gene="ydeO"
                     /locus_tag="R8G18_003288"
     CDS             3255..4016
                     /gene="ydeO"
                     /locus_tag="R8G18_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HEE9796905.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            4417..6099
                     /locus_tag="R8G18_003289"
     CDS             4417..6099
                     /locus_tag="R8G18_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEE9796906.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6151..7308
                     /locus_tag="R8G18_003290"
     CDS             6151..7308
                     /locus_tag="R8G18_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HEE9796907.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7274..7396)
                     /locus_tag="R8G18_003291"
     CDS             complement(7274..7396)
                     /locus_tag="R8G18_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796908.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
     gene            7611..9296
                     /locus_tag="R8G18_003292"
     CDS             7611..9296
                     /locus_tag="R8G18_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEE9796909.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            9334..11706
                     /locus_tag="R8G18_003293"
     CDS             9334..11706
                     /locus_tag="R8G18_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEE9796910.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            11763..14546
                     /locus_tag="R8G18_003294"
     CDS             11763..14546
                     /locus_tag="R8G18_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEE9796911.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            14908..>15149
                     /locus_tag="R8G18_003295"
     CDS             14908..>15149
                     /locus_tag="R8G18_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEE9796912.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4449 a         3299 c         3289 g         4112 t
ORIGIN      
        1 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgca gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccatgatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcagggcgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg
     1021 gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccac agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgta
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatt
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgtatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct gggtatttca
     3121 gaaatgggtc gcattgcaag agatcttaca catttcatta tcaatagttt gcaagactga
     3181 aaataaacag caaattataa atatgaaatg ttaaaaaagt atcgataaaa actttattgt
     3241 tttaaggaga taaaatgtcg ctcgtttgtt ctgttatatt tattcatcat gccttcaacg
     3301 ctaacatttt agataaagat tacgccttct ctgacggcga gatcctgatg gtagataacg
     3361 ctgttcgtac gcattttgaa ccttatgagc ggcattttaa agagatcgga tttactgaaa
     3421 ataccattaa aaaatatcta caatgcacta acatccagac agtgacggtg cctgttcctg
     3481 cgaagttttt acgtgcttca aatgtaccga ctggattgct taatgaaatg attgcttatc
     3541 ttaactcgga agaacgcaat catcataatt tttcagaact tttgcttttt tcttgcctgt
     3601 ctatttttgc cgcatgcaaa ggtttcatta cactattaac taacggtgtg ctatccgttt
     3661 ccggaaaagt gagaaatatt gtcaacatga agctggcgca cccatggaag ctgaaagata
     3721 tttgtgactg cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag caagagcaaa
     3781 cgacattctc acagattcta ttagatgcca gaatgcagca cgcaaaaaat ttgatacgcg
     3841 tagaaggttc agtcaataaa attgccgaac aatgtggtta tgccagtaca tcttatttta
     3901 tttatgcgtt ccgcaaacat ttcggcaaca gtccgaagag agtttctaag gagtaccgtt
     3961 gtcaaagtca catgggtatg aatacgggca acacgatgag tgctttagct atttgattat
     4021 ttgctaacga gtagtcaacc acgcacgctg cgtaagaatt aaatggggca gccattccct
     4081 gccccgcgtt gtttttaggc gatatattta ttgaaataaa taagtgacat ccatcacata
     4141 tttatgcact tgcataacct gttgcagatt atttatgatc tcaattcggc attttgtcag
     4201 taaaatgcaa taatttatta aatatcaata aattagttgt ttatcggcga gaaattactt
     4261 aatagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt tttagctaaa
     4321 tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct tcgtaaaatt
     4381 acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt aaagaaaagt
     4441 gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt tgctgctcat
     4501 gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc agattttacg
     4561 ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat ggatgatctt
     4621 ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat ggaaaatcgt
     4681 gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc acaaaaatca
     4741 acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg ctatgtggca
     4801 cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt
     4861 ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac tttcttgcct
     4921 gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca cttgtcaaaa
     4981 atcagtaatg tgccggtacc ggaagataaa caaacgcgtg actatcatga caacttcacc
     5041 acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt tatgggattc
     5101 cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc
     5161 cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt tgttgatcgt
     5221 gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc gcacctacca
     5281 aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag tcaaacagca
     5341 gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat tctcgaacaa
     5401 ctgaagaaaa acggacagta tgacaataca attattctct ttacctccga taatggtgcg
     5461 gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag tcagacctat
     5521 cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca acccggtaat
     5581 tatgacaagc tgatttccgc aatggatttc tacccaacag ctcttgatgc agccgatatc
     5641 agcattccaa aagaccttaa gctggatggt gtttccttgc tgccctggtt gcaagataag
     5701 aaacaaggcg cgccacataa aaatctgacc tggataacct cttattctca ctggtttgat
     5761 gaggaaaata ttccattctg ggataattac cacaaatttg ttcgccatca gtcagacgat
     5821 tacccgcata accccaacac tgaggactta agccaattct cttatacggt gagaaataac
     5881 gattattcgc ttgtctatac agtagaaaac aatcagttag gtctctacaa actgacggat
     5941 ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga gatgcaaggc
     6001 gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa tcaggagaag
     6061 tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca tgcggcggat
     6121 ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc ctccagtttt
     6181 caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca gtttactcat
     6241 gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat cgaagcgtct
     6301 ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg cctggatttt
     6361 ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat ttttaatgca
     6421 ttacaaacga atggcatttt attgaataat gaatggtgtg cctttctcaa agaacatgaa
     6481 tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta cagacgcagt
     6541 aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct gaaatcatat
     6601 caagtagagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta ccctcttgag
     6661 gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga attgctagaa
     6721 accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg tatcattgat
     6781 ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat gcaatgggtt
     6841 aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag ccgttttttg
     6901 gggaatgggc atacaagttg tattttccag gagtcctgca aggataatct ggttgttgaa
     6961 agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa aattggaaac
     7021 attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca aaaaaaacgg
     7081 atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg ttgtcctaag
     7141 catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg ttataaaatc
     7201 cttttttcaa ccatggtacc ttatatgaac gccatggttg agttagctaa gaacagagta
     7261 ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata atgctctctt
     7321 ttaactccat tcttagcctc cttcttatga ccttaacaag gcacccgaca taaattcctg
     7381 tcgggtgttt cgtcatcatc atcttccctt gcgaaaatcc tggcttacgt ggtatgcgtc
     7441 agggcattag caataccctt gattatcaat agcataacca taaccataac cataaccact
     7501 gaaaattaaa aatttctata tatttcatat tgttaaatga tacttaaata aatttcatca
     7561 gtaaatcatt gccatagata atcattggat ggcacaatca attttcgcta atgataacca
     7621 ttcccattac gttttatatg ttgatagcaa agtatctctg tctgctgaag cccttttggc
     7681 tgcgtaagaa taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg
     7741 gcgtggttaa aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa
     7801 gccagaaaga aaccgataag ctttggcagc ttgttctttg gttccctgcc ctgctgggga
     7861 tctttgtatt aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc
     7921 gcgaatggtt aacagattac taccttaatc gctggtttgc agataaaaac tactacttca
     7981 cgcagatcta cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca
     8041 ttctcttact gatcagcaaa acgctcagtc tttcatttgg cttcatccag tcacttagca
     8101 tgctgatcac ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg
     8161 gtggaacaga atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg
     8221 gtggaacttt atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac
     8281 aacgtagcga agcgacattt cgaactaatc ttgtgcagca taataagcag acagaactga
     8341 ttgcgctaag caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca
     8401 ttaaagagaa ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata
     8461 tttattcgcg ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg
     8521 ggcagatcaa tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca
     8581 atttaagctg gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc
     8641 gcttgtatga gttccatcaa ctcactgaac agcgccctac gaataagcct aaaaattgcc
     8701 aacatgcggt acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag
     8761 agaacttgaa ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg
     8821 cgggaaaaac cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata
     8881 tttcttctcc tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac
     8941 tgaaagagat tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag
     9001 tactgcatca ggttggtctg gggaaattgg ctgcgcgtat tcatgaccac gatcgctggg
     9061 gagatattct ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac
     9121 gtccgaaatg gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac
     9181 gcttactgcg tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc
     9241 aacccggtgt ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac
     9301 aataaaagcc ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc
     9361 attttatgtg gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgcatttt
     9421 tttgaagaga tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac
     9481 accagtgaaa gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga
     9541 accaaccctg cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt
     9601 cgccctgaga aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac
     9661 ggtattagtg caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc
     9721 aatattagcg ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg
     9781 ctttatgaca gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca
     9841 aagatcaaac gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt
     9901 tcggactggt taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggctct
     9961 tcaggaagta cttattactc cccagatttt aaaaagaact tttatacctt gtcgtttaat
    10021 caggaactcg ctgataactt cggcgttacc gccggtttat cgcgccgcca gtctgatatc
    10081 acccgcgcgg attatgtttc gaatgacggc attgtcaccg gtcgggcaca gtataaaaac
    10141 gttatcgata ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat
    10201 ttaaccttaa aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct
    10261 gatcgcgaaa tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca
    10321 tgggccaaac tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat
    10381 catgacatct ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc
    10441 tgtggcggat taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg
    10501 gactggcaaa aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac
    10561 atctattccg atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct
    10621 gcaggaaaga aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac
    10681 aactacacgc tgtatatggc ggagcacatt agctggcgga atgtgtcgtt aatgcccggt
    10741 gtgcgttatg actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg
    10801 gaatgggata tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat
    10861 ggcgggaata ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta
    10921 tcaggtaata aaactctgac gcgttatcag gatttgaaaa cgccttataa cgatgaactg
    10981 gcaatgggat tgcagcagaa aatcggtaag aacgttattg cgcgtgcaaa ctatgtttac
    11041 cgtgaagcgc atgatcaaat cagcaaaagc agtcgtaccg acagcgcgac taaaaccacc
    11101 attactgaat ataacaacga tggcaaaacc aaaacgcatt cgttcagcct cagttttgaa
    11161 ctggccgaac ccctgcatat ccgccaagta gatattaacc cacaaattgt ctttagctat
    11221 atcaagagca agggcaactt gtcgttaaac aatggttatg aggagagcaa taccggtgat
    11281 aaccaggtgg tttataacgg taatctggtc tcttacgata gtgttccagt ggcagatttt
    11341 aataacccat taaagatctc cttaaacatg gatttcacgc atcaaccgag cgggttagtg
    11401 tgggcgaata cgctggcctg gcaagaagcg cgtaaagctc gcattatcct tggtaagaca
    11461 aatgcgcaat acatcagcga atattcagat tacaagcagt atgttgacga aaaactggat
    11521 agcagcctga cctgggacac ccgcttgtcc tggacgccac aatttctgaa acaacaaaac
    11581 ctgacgatca gtgccgatat tctcaatgta ctggatagca aaaccgctgt tgatacaacg
    11641 aacaccggtg tggcgaccta cgccagtggc cgtactttct ggcttgatgt cagcatgaaa
    11701 ttttaatagt tcggatttta cttaaccggg taaattcgcc cggttttcgc atggagataa
    11761 ttatgagaaa cctctgtttc ttactgacgt tagtggcaac tctgttgctc cccgggcggc
    11821 tgattgccgc cgccttaccg caggatgaaa agttaattac cgggcaactg gacaatggct
    11881 tgcgatatat gatttatccg catgctcaac caaaggatca ggtaaattta tggctgcaaa
    11941 ttcataccgg ttcattgcag gaagaagaca atgagcgcgg cgtggctcat tttgtagaac
    12001 atatgatgtt taacggcaca aaaacatggc cgggtaataa agtcatcgaa acatttgagt
    12061 caatgggcct gcgtttcggt cgcgatgtta atgcctatac cagctatgac gaaacgctgt
    12121 atcaggtgag tctgccgact acgcagaaac aaaatctgca acaagtgatg gcaatcttca
    12181 gtgaatggag taatgccgca acctttgaaa tactcgaagt agacgctgaa cgcggcgtaa
    12241 ttactgagga atggcgtgcc catcaggatg cgaaatggcg cacctctcag gcgcgccgcc
    12301 ctttcctgct ggcaaatacc cgtaatttag accgtgaacc tatcggcctg atggatactg
    12361 tcgccacggt cacaccggca caattgcgcc aattttatca acgctggtat caaccaaata
    12421 atatgacctt tatcgtggtc ggcgatatcg acagtaaaga agcgctggcg ctgataaagg
    12481 ataatttaag taagcttccg gctaacaaag cagctgaaaa tcgcgtctgg ccgacaaaag
    12541 ccgaaaacca cctgcgcttt aatatcatta atgataaaga aaaccgagtg aacggcatcg
    12601 cactctatta tcgcctgcca atggtacaag tgaacgatga gcaaagcttt gtcgaacaag
    12661 ctgaatggag catgttagtt cagctgttca atcaacgtct gcaggaacgc atacagtcgg
    12721 gcgagttgaa gactatttct ggcggcactg cgcgcagcgt taaaattgca cccgattatc
    12781 agtcgctgtt tttccgtgta aatgcacgag acgataatat gcaggatgct gcaaatgcat
    12841 taatggcaga gttggcaacc attgatcagc atggtttttc tgctgaagaa ctcgatgatg
    12901 tcaaatctac ccgcctcacc tggctgaaaa atgcggttga tcagcaagct gaacgtgatt
    12961 tacgtatgct gaccagtcgc ctggcatcca gctcattaaa taatacgccg ttcttgtcgc
    13021 cggaagagac atatcaactt tcgaaacgtc tgtggcagca aattaccgtg caaagtctgg
    13081 cggaaaaatg gcagcagtta agaaagaacc aggacgcatt ttgggagcaa atggtaaaca
    13141 atgagcttgc cgccaaaaaa gcattgtctc ttgcggctat cctggcgctg gaaaaagagt
    13201 acgccaacaa aaagctggcg gcttacatct tcccaggcag aaatttatcg ttaacagtag
    13261 acgctgaccc acaggcggaa attagcagca aagaaacgct ggctgagaat ctgacatcat
    13321 taacactttc caatggtgcc agggttattc tggcaaaatc cgcgggtgaa gagcaaaagc
    13381 tacaaattac tgccgtatct aataaaggcg atttaagttt ccctgcgcag caaaaatcac
    13441 ttatcgcgct ggcaaataaa gcagtaagcg gaagcggcgt tggtgagctc tcctcttcca
    13501 gcctgaaacg ctggagtgcg gaaaattcgg taaccatgag cagtaaagtc agtggcatga
    13561 atacgttgct ctccgttagc gcgcggacta ataaccctga acctggtttt cagttgatta
    13621 accagcgaat cacccacagc acgattaacg acaatatttg ggcatcgcta caaaatgctc
    13681 aaattcaggc gttgaaaacg ctcgaccagc gtccagcgga gaaattcgcc cagcagatgt
    13741 atgagacgcg ctatgctgat gaccgcacga aattactgca agaaaatcag attgtacagt
    13801 ttactgccgc agatgcgctg gctgccgatc gccagttgtt ttcatctcca gcggatatca
    13861 cgtttgtcat tgtcggtaat gtctcagaag acaaactcgt ggcgttaatt acgcgttact
    13921 taggatcaat caaacactct gattcgccat tagccgcagg taaaccatta actcgcgcga
    13981 cggacaacgc atcggttact gtaaaagaac aaaatgaacc tgtggcacag gtttcacagt
    14041 ggaagcgtta tgattcccgg acacctgtta atctggcgac gcgtatggcg ctcgatgctt
    14101 ttaacgtcgc actggcaaaa gatctacgcg ttaatattcg tgaacaggca tctggagcat
    14161 acagcgtttc ttctcgcctc tcggttgatc ctcaggccaa agatatcagt catttgctgg
    14221 cttttacttg tcaaccagaa cgacatgatg aactgttaac gttagcgaat gaagtgatgg
    14281 ttaagcgtct ggctaaaggg atcagtgagc aagaactgaa tgaataccag caaaacgttc
    14341 agcgcagcct cgatatccaa cagcgtagcg ttcaacaatt agcgaacact attgtaaata
    14401 gtcttattca atatgacgat cctgcagcat ggactgagca ggagcaattg ttgaaacaaa
    14461 tgacggtaga gaatgttaac actgccgtta aacaatatct ttctcatccg gtaaatactt
    14521 atactggagt attattgcca aaataataac agccccgtca acacatcgtt ggcggggatt
    14581 ttagcaatat tcgccatttt tatgtaataa ttttataaat gcgttcaaaa taataaccag
    14641 gtactaatag tgatatttta aggtctgatt tttacgtgaa aattcaggag gcacagaatg
    14701 cgcataaaaa taacagcata aaacacctta ccaccaccca agaatttcat attatattgt
    14761 ttttcaatga aaaaatatta ttctcgtaat atctcacgat aaataacatt aggattttgt
    14821 tatttaaaca cgagtccttt gcacttgctt actttatcga taaatcctac ttttttaatg
    14881 cgatccaatc attttaagga gtttaaaatg gataagaagc aagtaacgga tttaaggtcg
    14941 gaactactcg attcacgttt tggtgcgaag tctatttcca ctatcgcaga atcaaaacgt
    15001 tttccgctgc acgaaatgcg cgacgatgtc gcattccaga ttatcaatga cgaattatat
    15061 cttgatggca acgctcgtca gaacctggcc actttctgcc agacctggga cgacgaaaat
    15121 gtccacaaat tgatggattt atccattaa
//
LOCUS       DAQSWQ010000104        15079 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.105, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000104 DAQSWQ010000000
VERSION     DAQSWQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.105"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            13..128
                     /gene="rrf"
                     /locus_tag="R8G18_003296"
     rRNA            13..128
                     /gene="rrf"
                     /locus_tag="R8G18_003296"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            454..1752
                     /gene="kgtP"
                     /locus_tag="R8G18_003297"
     CDS             454..1752
                     /gene="kgtP"
                     /locus_tag="R8G18_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HEE9796913.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1749..2072)
                     /locus_tag="R8G18_003298"
     CDS             complement(1749..2072)
                     /locus_tag="R8G18_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HEE9796914.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2118..3473)
                     /gene="pssA"
                     /locus_tag="R8G18_003299"
     CDS             complement(2118..3473)
                     /gene="pssA"
                     /locus_tag="R8G18_003299"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEE9796915.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3587..6247)
                     /gene="pat"
                     /locus_tag="R8G18_003300"
     CDS             complement(3587..6247)
                     /gene="pat"
                     /locus_tag="R8G18_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HEE9796916.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6279..6977)
                     /gene="tapT"
                     /locus_tag="R8G18_003301"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6279..6977)
                     /gene="tapT"
                     /locus_tag="R8G18_003301"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEE9796917.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7046..7465)
                     /gene="trxC"
                     /locus_tag="R8G18_003302"
     CDS             complement(7046..7465)
                     /gene="trxC"
                     /locus_tag="R8G18_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEE9796918.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7672..8709
                     /locus_tag="R8G18_003303"
     CDS             7672..8709
                     /locus_tag="R8G18_003303"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HEE9796919.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8757..9446)
                     /gene="ung"
                     /locus_tag="R8G18_003304"
     CDS             complement(8757..9446)
                     /gene="ung"
                     /locus_tag="R8G18_003304"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEE9796920.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9751..10134
                     /gene="grcA"
                     /locus_tag="R8G18_003305"
     CDS             9751..10134
                     /gene="grcA"
                     /locus_tag="R8G18_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HEE9796921.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10190..10777)
                     /gene="eamB"
                     /locus_tag="R8G18_003306"
     CDS             complement(10190..10777)
                     /gene="eamB"
                     /locus_tag="R8G18_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HEE9796922.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10880..11761
                     /locus_tag="R8G18_003307"
     CDS             10880..11761
                     /locus_tag="R8G18_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9796923.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11970..13304)
                     /gene="srmB"
                     /locus_tag="R8G18_003308"
     CDS             complement(11970..13304)
                     /gene="srmB"
                     /locus_tag="R8G18_003308"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HEE9796924.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13436..14173
                     /gene="trmN"
                     /locus_tag="R8G18_003309"
     CDS             13436..14173
                     /gene="trmN"
                     /locus_tag="R8G18_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HEE9796925.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14158..>15079)
                     /locus_tag="R8G18_003310"
     CDS             complement(14158..>15079)
                     /locus_tag="R8G18_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEE9796926.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3638 a         3818 c         3956 g         3667 t
ORIGIN      
        1 aaacagaatt tgcctggcgg ccgtagcgcg gtggtcccac ctgaccccat gccgaactca
       61 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
      121 tgccaggcat caaattaagc gaaaggccat cctgacggat ggcctttttg cattggcgca
      181 gaaaaaaatg cctgatgcga cactgcgcgt cttatactcc cacatatgcc agattcagca
      241 acggatacgg tttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat
      301 ccttaagctc ctcaataacc attttcctgc taactaaatt catggttaag gttgcacaat
      361 gatatgcaac aaatgtataa catttccttt accaaaaaaa ataaacaaaa gcgaccgaca
      421 aaagcatcgg attacggcag gagacataat ggcatggctg aaagtactgt aacggcagac
      481 agcaaactga caagtagtga tactcgtcgc cgcatttggg caattgtggg ggcctcttca
      541 ggtaatctgg tcgagtggtt cgatttctat gtctactcgt tctgttcact ctacttcgcc
      601 cacatcttct tcccttccgg gaacacgacg actcaactac tacaaacagc aggtgttttt
      661 gctgcgggat tcctgatgcg cccaataggc ggttggctat ttggccgcat agccgataaa
      721 catggtcgca aaaaatcgat gctgctatcg gtgtgtatga tgtgtttcgg ctcactggta
      781 atcgcctgtt tgcctggtta tgaaacaatc ggtacgtggg ctccggcatt attgcttctc
      841 gcgcgtttat ttcagggatt atccgttggc ggagaatatg gcaccagcgc cacctatatg
      901 agtgaagttg ccgttgaagg gcgcaaaggt ttttacgcat catttcagta tgtgacgttg
      961 atcggcggac aactgctagc cctactggtt gtcgtggttt tacaacacac catggaagac
     1021 gctgcactca gagagtgggg atggcgtatt cctttcgcgt taggagctgt gttagctgtt
     1081 gtggcgttgt ggttacgtcg tcagttagat gaaacttcgc aacaagaaac gcgcgcttta
     1141 aaagaagctg gatctctgaa aggattatgg cgcaatcgcc gtgcattcat catggttctc
     1201 ggttttaccg ctgcgggctc cctttgtttc tataccttca ctacttatat gcagaagtat
     1261 ctggtaaata ctgcgggaat gcatgtcaac gtggcgagtg gcattatgac tgccgcattg
     1321 tttgtattca tgcttattca accactcatt ggcgcgctgt cggataagat tggtcgccgt
     1381 acctcaatgt tatgtttcgg ttcgctggca gccattttta ccgttcctat tctctcagca
     1441 ttgcaaaacg tttcctcgcc ttatgccgct tttggtctgg tgatgtgtgc cctgctgata
     1501 gtgagttttt atacatcaat cagtggaata ctgaaggctg agatgttccc ggcacaggtt
     1561 cgcgcattag gcgttggcct gtcatatgcg gtcgctaatg ctatatttgg tggttcggcg
     1621 gagtacgtag cgttgtcgct gaaatcaata ggaatggaaa cagccttctt ctggtatgtg
     1681 accttgatgg ccgtggtggc gtttctggtt tctttgatgc tacatcgcaa agggaagggg
     1741 atgcgtcttt agtgacgggt cagttgccag acggtatagc cggtgcttgc accggcgaca
     1801 tcccaggcca aatccttcca gctccagccg ctcccttcgg ggcggctatc ccaaagctct
     1861 tttgacgccc ccaaactgac agagaacatc aatccaaaca tggcactgcg atcccggctc
     1921 atcccctgat gctgtgaata ttcatttccg gcggcggaaa gcatcgccga ggcgataaag
     1981 tgttgagctt tatcctgccc gctccagcta tcgttagcca tatggctgca tccagaaagt
     2041 aacaatagcg aacagacaaa aagaatacgc aaaacgtggc ctccaaaaaa caaaccccgt
     2101 acaggacggg gttgtgatta caggatgcgg ctaattaatc ggtcgatgcg gatacggcgc
     2161 aaacggcgga tgagtttacg aaccttcacc ggataatcgg caatactttg cagatcgcga
     2221 tagtgcttaa cgatggtggt atgctcgcgg atcagctcca gttctttctc tcgctgcggc
     2281 gcaagcttca gttgtggatc gtggatcaaa atggcgtttt ccagatccag acgccaggcg
     2341 cgcgggttca ggttattacc ggtgatcaac atccacttat catcaaccca catccctttc
     2401 aggtgatagg tgttgtcgtc atctttccat aaccgaacca ctagctggtc agtattgacg
     2461 taatactgca aacggctcag gaaacgacgc agattgatct catagagata aggcaatgcg
     2521 ccaattatct tgaaaggttc atcttccgga atgtagaagt cgttcgcggt tttatcacca
     2581 acaataattt cgaccttttt cccttcgcgc agcaactgga taatattgcg cacaaggatt
     2641 gctggcaggt tgaagtatgg cgtacagatg gttagtttct gctcggcaca aggcataaga
     2701 tggaaaatgg tcttgttcaa cagactcgat ttccccagcc ccactagcgg cgttacggaa
     2761 agctgatcgt tgtcggcatc gccctggaaa tgataagcgg catcacgcag ctcctggcgg
     2821 aacagacgaa tatcgttctt gatttccggg ctttttggcc gattaacatc atccagacga
     2881 ttaacgccgc ggccattcat aatattctgt gtaacccatt caaacataat gtctgacatc
     2941 ttacggttac ggataagatg ataacggtcg tagcgatatt tatcgtgctg atgcaggtaa
     3001 acatcgttca ggctggcacc gctataaagt acgctatcgt cgatgataaa gcctttaaag
     3061 tgcagaacac caagggcttc acgggtattg attggaacgc cataaaccgg aacatctacg
     3121 cccgaatttt cctgcgccat gcggcagtac cagtcagcgt tagtgttaga tgccgcagcg
     3181 ccaatgcgtc cacgttgtgc acgatgccag tcgaccagca cccgcacatc cagttccgga
     3241 cgctgccttt tagcttcata caacgcgttc agaatgcctt tgccaccgtc atcctgttcg
     3301 agatacaggg cgacaatgca aatgcgctgc ttcgcgctgg ctattttttc cagcagcgtc
     3361 tcccggaagt cggcgggagc gtaaaagaaa tcgacatcat caactgattg agaaatcttg
     3421 ggtagttggg caaggtgttg ttgatgttta ttacgcttaa attttgacaa catcacagtg
     3481 catttcttct ctgttcattg aagggtcctc tgtgcaatgc agacgacata agcgggcaat
     3541 aataacacca gtcccgatta agtggtcaac atttccagta ccttactcat gattcctcgc
     3601 gttgggcaag atttagcgta agcccaacga tcccctcttc gagctggata tcaacgttaa
     3661 acccgagctt gcgggctagc gccaccatgc cacgattgtt tggcatcgta ataccattca
     3721 gacgttgtag tccgtgatct cgcgtatagg taatcaactt ttccattaag cgtcgaccta
     3781 agcctaaccc tttgagatcc gagcgaacca gcacagcaaa ttcggcatcg atgttatcag
     3841 gatcggaaat cgcacgcgtg acgccgagga tctcttccgt ttgatcaata cgtcgtaccg
     3901 ctacaaacgc catttcccga tcgtagtcga tctgtgtcat gttggctaaa tcttcatggg
     3961 taaattcgtt gatctcgcta aagtagcggt aataaagatc ttctttggtg actcgcgaaa
     4021 tgaattgctg aagttgtggc tcatcttctg gcaaaatcgg gcggaacaag cagcgttcac
     4081 cgtttttcaa ttctacccat tcttccagct gatgcggata agggcgcact gccagccgac
     4141 tctcgttatc gccttcaaac ggcgagatat ccagcgtgac atccagcgcg gtaaattcac
     4201 tgccagaagc cagcaaagga tgaatatcca gacgctgaat ttccgggcaa tcgacaatca
     4261 agttggaaac ctgcaccaga agctggctca agcctgcaac atccaatggg cgtagcgcac
     4321 tgcgcgcacg aatcttttta cttttgatcc cctgaataac cagatagcgg gccaggttca
     4381 tgttcagcgg cggcagtgcg acgacggctt gatcttcagg acgccactcc acaccgcctt
     4441 cacccagcat gatcaacggc ccgaaaaccg gatcgtgctc aaccacaacc cgcaactcct
     4501 gagcgccagc acggttagcc atactttgca ccaacaggcc gtggacccgc gcctgtggcc
     4561 aggccatttt tacgcgatcg aaaatagcgt tcgccgcttg ctggacttca ttggctgtac
     4621 gcaggtaaag catgacgccc tgaacttccg atttatgtgg aatatccggc gaacgcaatt
     4681 tcagcgccac cggataacca atctgttcgg caatatgcac cgcttcggtg ctatcgctgg
     4741 caatccaggt agggagcgtg ttcatgccat acgcttgcag gatgggctga acttcatggg
     4801 tatcgagcga cgtagcccct tcggcaatcg cctgttgcaa cagaagatgc gcttctgcgg
     4861 tattggaagt cagattgctg ggcaacgtcg gcgtttcgcg tagttgcttc tgattacgcc
     4921 ggtactccac catatgcata aaagcagtga tggttccttc cggggtacgg taggtcggca
     4981 gcccggcttc gctgaataaa cgtcgtgcct cttgcgagga gtgctcgccg caccagttcg
     5041 tcagcaaaga gacatatttg ctgcggggat gatgctttac cgcttcaatt aatacttgcg
     5101 cgctttctgt tgcgggagca gcggcgctgg gcgaatgaat aaccatcagc gcatcaaaat
     5161 cctggctgtg gagcagaatg tccagcgttt tgacataatg ctcgctgctg gcgtcatcgc
     5221 gcagatcgag agggttagag actgccacat gttctggcaa tgcatcgcgc aatttctggc
     5281 aggtttcttc gcttagcgtt gccagcttgc cattgcgtga ccataaggca tccagcgcca
     5341 gcgcggcagg cgcagcaccg ttgctgataa tcatcaaccg gtcgccacgc agcgggcgca
     5401 tatggctaag ggtttccacc gccgaaaaca gttcgtgggt atcctgtacc cgcaacaaac
     5461 cggcacgctg aatagccgca tcccatgccg ggtccattcc tgccgtcgtg ttgagcagtc
     5521 gctgtgccgc cgggctacgt ccgcttttaa tcaccagaat cggtttatta cgcgaggcac
     5581 tacgggctgc cgaaacaaag cgtcgcgcgt cgcttaactg ttcgagatag agcaggatgg
     5641 cgctggtttt actgtcgcgc gccagatagt cgagcaattc atcaacgtcg atatccaggc
     5701 tgtcgccgag cgcaataaag taggaaaagc ccatctcacg ctgttgcgcc cagtcgagga
     5761 tggtgttgga gacggctgcc gattgtgaaa taaacgccag cttgccgcgt ttaatcggca
     5821 caggcgaaaa gctggcattc agaccttgcc agggagccag taaacccaga ctgtttggtc
     5881 caagcaggcg catgttatgg cgcagggcgc aggcgcggag atcttcgtgt tgcgatgccg
     5941 gggcggaaag aataatgcag gttttacagc ctttctcgcc gagctcttcc agaagagcaa
     6001 gattacggct ggcattggta cataaaaccg caaggtcggg tgtaaagggc aagctggcaa
     6061 tatccggcca ggccaacaca cccaacaccg ctttccaggc tggcgtcacc gggagtaccg
     6121 gtccgttaaa gcttcccgcc agcaggttac gcatcatcag gtaacctgcg cgattgggtt
     6181 tcatcgacgc gccaattacc gctatcgatt ttggtcgcag tagtgcttcc agtcctcgct
     6241 gactcatacc ggtctcccgc agaagtgacc gaatgatttt aaacgctttc taactgttct
     6301 gctgtgatgc tacccagatg ttgcgttttt cctgccagat agcgtgtttt aaagcgggta
     6361 aaatgctcgc ctaaccctgc tgccgccccg gtatcgccgg ccatatctaa cagtgcgatg
     6421 gctacctcgg cagtacaata ttggcctgca gcctgggctt cacgcaggcg ataggcagaa
     6481 agccgggaaa gatcgacgga aatgacggga agattatcca gatacggact tttacgaaac
     6541 atcttgcgag cttccggcca ggtaccatcg agcatgataa acagcggtgg cttaccggca
     6601 ggtggtgtga agatcacttc ccgttgctca tcagcatacg aggcgggaaa gaccaccatt
     6661 ggctgataat ccgggttttg taccagatcc agcaaatcct gcgagggttc ggtacgcgac
     6721 cattgaaacg caacggtatc aggcaaaata tcagcaatca gacgcccggt attactgggc
     6781 ttcattggct cggtgtcgaa catcagcaaa cagaagcgac tttttgcttg tgctggggta
     6841 attgtcgaac agagacataa tttctctggc aaaagacagc gttggcagcg atgaacgcga
     6901 ttaccgcggg caagaaaagg acgtgttgcg cgcgcaatac gctcggcgcg taactggaga
     6961 acagcgtttt cggtcataag agagcgtcga aaaaacgcca ttgtcgcaga ggagaaaacg
     7021 gggcacaaga tgcaccccgg taagattaaa gagattcgtt cagccagcta tcgaacggcg
     7081 ctttcggtac tgcgccatta agcatgtcga caacctgacc gtttttgaaa atcatgatcg
     7141 tcggtatact acgaattcca aagcgactgc tcaattcacg ttcagcttcg gtattcactt
     7201 tcacaaagcg cactttaccg ctacgctctt gcgcgacatc ttcaaaaatt ggtgcgaaat
     7261 tacggcaggg gccgcaccac ggtgcccaga agtcgatcac cacaggtaga tcatccttca
     7321 gcaatttgtc gagtgtttca ccggtcgcat taatcacctc tccgtcaaac aagtcgtgac
     7381 cgcagcgtcc gcattttgcc gcatcttcga tccgatcgtc gggaatgcga ttgatggcct
     7441 gacaatgggt acaaacggta ttcataacta acctcgggat gagtaaggag acttatcggc
     7501 aacgacgccg gtatgtttct aatatgttac atattatcgg ggacattgtt ccagacaaca
     7561 atctgtttta ttcaataggt atgatagtcg caggctttgg tacgaaatca tggcgaaccg
     7621 cgcggacttc gggtaatctg cgcgcttcgc gcagcgctgg tggagaaaag catgaacgat
     7681 gaaatgaaag gtaaaagcgg caaggtcaaa gtgatgtatg tccgcagtga tgatgattct
     7741 gataaacgta cccacaaccc gcgtaccggg aaagggggcg ggcgtccagg aaaatctcgt
     7801 gctgacggtg gccgtcgccc cgcccgcgat gacaaacaga gtcagccccg tgaccgcaag
     7861 tgggaagatt cgccgtggcg cacggtttcc cgcgcgccgg gtgatgagac gccggaaaag
     7921 gccgatcacg gtggcatcag tggtaaaagt tttattgatc cggaagtgtt gcgtcgtcag
     7981 cgtgcggaag aaacccgcgt ctacggcgaa aatgcctgtc aggcactgtt ccagagccgt
     8041 ccggaagcaa ttgttcgcgc ctggtttatc cagagcgtaa cgccacgttt taaagaagcc
     8101 ttgcgctgga tggcggcaaa ccgcaaagcg taccatgtgg tggatgaagc tgaattgaca
     8161 aaagcgtcag gcacggaaca tcacggcggc gtttgcttcc tgatcaaaaa gcgtaacggt
     8221 acaaccgtgc agcagtgggt aagccaggca ggcgcgcagg attgcgttct ggcactggaa
     8281 aatgaatcta acccgcataa cctgggtggc atgatgcgca gctgcgcgca cttcggcgtg
     8341 aaaggtgtcg tggtgcagga tgcggcactg ctggagtcgg gggcggctat ccgtaccgca
     8401 gaaggcggcg cagagcacgt tcagccgatt actggcgaca acattgttaa cgtgctggat
     8461 gatttccgtc aggcaggtta caccgtagtg accacttcca gcgagcaggg taaaccgctg
     8521 ttcaaaacta gtctgccagc gaaaatggta ctggtgctgg gtcaggaata tgaagggtta
     8581 ccggatgccg cacgcgatcc gaacgatctg cgcgtgaaga ttgacggtac gggcaacgtt
     8641 gccgggctga atatctctgt cgcaaccggc gttttgcttg gtgaatggtg gcgtcagaat
     8701 aaagcctgat aaatcagccg ggtggcaact ctgccatccg gcatttcccc gcaaatttac
     8761 tcactctctg ccggtaatac tggcatccag tcaatcggcg tctcgccacg ttgttccagc
     8821 cactgatttg ccagcacaaa atggttgcag ccaaagaatc cacgatgcgc cgaaagcggc
     8881 gacggatgcg gtgctttcag tacatgatgg cgttgcttat ctataatcgc ccctttcttt
     8941 tgcgcatgtg atccccacaa caaaaacacc acgccttcgc ggtgctggtt aatcaggctg
     9001 atgactttat cagtaaacgt ctcccaaccg aggctggcgt gggaatgcgc ctgacctgcg
     9061 cgaaccgtca acacagtatt gagcagcaga acgccctgac gcgcccagct ttcgagatag
     9121 ccatgattag ggcgggtgaa gcccggaata gtattttcca gctctttata catattcaat
     9181 aatgacggag gaatggcaat gccgggacga acggaaaatg ccagaccatg cgcctgtccc
     9241 ggtccgtgat aaggatcctg gccgagaatc accactttaa cgtcacccaa ctcggtaaag
     9301 cggaacgcgt taaagacatc tttttgtggt ggatagatag tgacgccgga ctgccgctcg
     9361 ctggcgacgg tctgaagggt attaagaaaa taaggttgtt gcttctcttc agccagcacg
     9421 tcatgccagg ttaattcgtt agccatctcg ctctcctgcg aatcttcaat ccgcctagct
     9481 taactgcttc tttctacaga acaaaatcat gctcattttt tagacttaaa cgagtgcaaa
     9541 aaaatgtaaa ttggtaaaat ttcacacccg cataagttga tgtaaaacaa taaaatccat
     9601 ttacgaccac aatcgatgtg tggtttttat tgatttaaat caaagaatca agggtggttg
     9661 aggagtatat atacactcaa gcaacaatgg ttttaccaat tggccgcgac aggctgaaca
     9721 aatcaaataa ttttgccggg gaggcatcac atgattacag gtatccagat tactaaagcc
     9781 gctaacgacg atctgctgaa ctctttctgg ctgctggaca gcgaaaaagg cgaagcacgt
     9841 tgcatcgttg caaaagcagg ttatgcagaa gatgaagtgg ttgcagtaag caaactgggt
     9901 gacattgaat accgtgaagt tccagtagaa gtgaaaccag aagttcgcgt tgaaggtggt
     9961 caacacctga acgttaacgt tctgcgtcgt gaaactctgg aagatgcagt taagcatccg
    10021 gaaaaatatc cgcagctgac catccgtgta tccggttatg cagttcgctt taactctctg
    10081 actccggaac agcagcgcga cgttatcgct cgtaccttta ctgaaagcct gtaaggctta
    10141 agtagcaagt tactaagcgg aagagggcga cctcttccgc tttttttcgt taatagaaaa
    10201 tgcgtaccgc gcaatagacc agtaacaggg caagcacgat atttaactgg cgaccatact
    10261 ggcgaaacaa tcgctgaaac agatgccccg ccagcgccca gcacacattg ccaaacgtcc
    10321 caatcatcgc cagcaaaacg ctgacgccaa ctacccagct tagcgcctgt gtttgcggca
    10381 gaacaaacgt cgacagtgcc gtaacaccgt acaaaatgat tttgacgttc acaaactgca
    10441 aagcaaagct ggcccaaaag ctgattggtt ttgcctgaag tccgtcttcc tttgttgggc
    10501 tggtggcgat tttccacgcc agccagacaa tatatgccgc ccccgcccaa ctcaaaaggt
    10561 gtaccgctgc cgggtcaatc actgccagtg aaaatgaaat gcccgcacac agtaacatca
    10621 caatcaaaaa tcccagactc atccctgcca gcacgcgggt actttgacga aatccatgcg
    10681 acgtagcaga gctaagggcg agaatattgt tcggtcctgg cgtcatagcg gtaatcaggg
    10741 tgtaagtcca aaaagcactt aaaagggtcg gtgtcacttg ttatctcctc tgttttggta
    10801 gacagtaccc gacacggaac atttaaaaaa atgaatgttt ttatattcta gattcgatat
    10861 tttcgatagg tttggggtta tggatctgcg ccgttttatt acgcttaaaa ccgtggtgga
    10921 agagggttcc tttttgcgag cttcgcaaaa attgtgctgt acacaatcga cggtgacttt
    10981 tcatattcag cagcttgagc aggagttttc agtccagtta tttgagaaaa ttggccgacg
    11041 gatgtgcctt acccgcgaag gaaaaaagtt gctgccgcac atttatgaac ttacccgagt
    11101 gatggatacg cttcgtgagg ctgccaaaaa ggagtcagat ccggacgggg aactacgcgt
    11161 tgtttcgggc gaaacgttgc tctcttaccg aatgccgcag gtgttgcagc gttttcggca
    11221 acgcgcacca aaagtgcgtt tatcgttgca gtcgctgaac tgttacgtga tccgtgatgc
    11281 gctactgaat gatgaggccg atgtcggcgt cttttatcgc gtagggaatg atgatgccct
    11341 gaaccgacga gagttgggtg aacaatcgct ggtgctggtg gcttcaccac aaattgtaga
    11401 tgtcgatttt actgagccgg gaagacataa cgcctgtagc tttattatca atgaaccaca
    11461 atgtgtcttc cggcagatat ttgagagcac gctgcgtcag cggcggatca cggtggaaaa
    11521 caccattgag cttataagta tcgaaagcat caagcgttgt gtcgcggcga atatcggagt
    11581 cagctacctg ccgcgttttg cggtggcaaa agagttggag tgcggagaat taattgaatt
    11641 gccttttggc gaacagtcgc agaccattac ggccatgtgc gcacaccatg ctggaaaagc
    11701 ggttagcccg gcgatgcaca cttttattca gtgtgttgaa gagagttttg tggctggata
    11761 aaaataatgt cgggattaaa cccggcattt aacttgtcga caaagtgcgc tttattcatg
    11821 ccggatgcgg cgtgaacgcc ttatccggcc tacaagatcg tgcaaattca atatattgca
    11881 tgagccacgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg ccagcacttt
    11941 taataccagg cttaaacccg gcattgagat tactcttctg tcgtttgcgg tggcacgccc
    12001 gttccgcttg gtttacggcg cttaccaata tttttggtgt cgcgatggcg ttttttcacc
    12061 cgcggtttct ctttctcttt agcttttttc ttctcggcgc gttttgccag tactttcttc
    12121 gatggcttgc cggtctgctt ctcgcttggc gcacgcgttt tcgggcgtaa ctcatcaata
    12181 acgcgagctt taatcggctc ttcaatgtaa cggccaacct tgcccagcag caaatggtca
    12241 tgggcttcca ccagcgaaat tgcggtgcct ttacgaccgg cgcgcgcggt acgtccgata
    12301 cggtgcaaat aagtatcgcc actgcgcggc atatcgaagt taaagacgtg gctgacgtca
    12361 ggaatgtcga taccgcgcgc ggcaacatcg gttgcaacca gtacgtttac gcgaccttcg
    12421 gtcaaacgct tgatcgcttc gttacgcttg ccttgtacca tctcaccttc gagatagcag
    12481 ttgttgatgc ccgcttcgcg cagccagttt gccagctcat gcacacgttc acgcttacgc
    12541 acaaacacaa ttgagcgggt cgcttccggc tgttttaaca gatgcaccag caatgcggtt
    12601 ttatgttcca ggtcatcggc gcggtaatac cactgatgaa tttttttgcg ctcacgggtg
    12661 gacggattgg cagaaacctc caccggatct tccagcagac gctcggcaaa gtcctgaatc
    12721 gcatcgcctt ccagcgttgc cgaaaagagc agggtctgtt tacgccagcg cgtttcgcca
    12781 gcaatatgtt cgatatcctg agcgaagccc atatccagca tacggtctgc ttcgtcgagg
    12841 atcagcgttt caaccgcgcg gcaatcgaag ttctcttctt ttatgtattg cagcagacgt
    12901 ccggtcgtgg cgaccacgat gtcctgattt tcgctgaaca cttccgcgtg gttcatatag
    12961 gctacgccgc cggtgatggt ggcgatatcc agatgcgtat gtttcgccag ttcgcgggca
    13021 tgatcggcca cctgcatcgc cagctcgcga gttggggtga ggatcaaaat acgcggcgga
    13081 ccggatttct tacgtgggaa atcgagcagg tgctgcaacg ctggcagcag atacgccgcc
    13141 gttttaccgg tgcctgtcgg cgcagaaccg agtacatcac ggccatcgag cgcaggcgga
    13201 atggcggcag cctgaatggc ggtcgggcga gtgaaacctt tatcctggag ggcttccagc
    13261 aggctttcgt cgagttcaag ttcggaaaaa gtcgttacag tcatgttcta cctctgtgtg
    13321 gggcgctgat tatagacgtt acggctgcaa tcttcatctg tttgtatgga tatcgcttct
    13381 cgggtattgt tctcacccgg tgctatcgcc gtttttcctg caaggtttta ttttcatgtc
    13441 acagtctaca tccgtgcttc gtcgtaatgg atttactttt aaacagtttt ttgttgctca
    13501 cgatcgctgt gcgatgaaag tgggaacgga tggcattttg ctgggcgcat gggcaccggt
    13561 ggctggggtg aaacgttgcc ttgatatcgg cgcgggtagc gggttgctgg cactgatgct
    13621 ggcgcagcga accagtgaca gcgtgataat tgatgccgtt gagctggaaa gtgaagctgc
    13681 cgcgcaggcg caggaaaata tcaaccagtc cccgtgggca gagcggatta acgtccatac
    13741 ggcggatatt cagcagtggc tcacacagca gacagtacgc ttcgatttaa tcatcagtaa
    13801 cccaccttac tatcagcagg gagtagagtg cgcgacacct caacgggaac aggctcgcta
    13861 taccactacg cttgatcacc catcgctgct gacctgtgcg gcggagtgca ttaccgaaga
    13921 gggatttttc tgcgtggtgc tgccagagca ggttggtaat ggttttactg aactggcatt
    13981 aagcatgggc tggcatttac gtttgcggac ggatgtggcg gaaaacgaag cgcgactgcc
    14041 gcatcgggtg ctgctggcat tctccccgca ggcgggagaa tgctttagcg atcgcttagt
    14101 cattcgtggg ccagaccaga actattccga agcgtatacg gcgctgaccc aggcttttta
    14161 tctgtttatg taatgattgc cggggggaag gatcgatgga ccggaatgcg tgagcagttc
    14221 cgggtaatcc agcgtaaaat gcaggccacg actctccttg cgcatcatcg cacagcgaac
    14281 aatcaactcg gcaacctgta ccagattacg cagctccagc aaattattcg acacgcggaa
    14341 gtgggcgtaa tattcgtcta tttcctgttg caacatattg atacgccgca gggcgcgctc
    14401 aagacgtttt gttgtgcgca caatgccgac gtaatcccac ataaacagcc gtagctcgtg
    14461 ccagttatgc tgaattacta cccgttcgtc agggttctca acgcggcttt catcccacgg
    14521 tggtaacgta ctgacgccgt gggcatcagg catacgtctg gtgatatctt ctgccgccga
    14581 ccagccatag accagacact ccagcaatga attcgaggcc atgcggttag cgccgtgtaa
    14641 gccggtataa ctcacctcgc caatggcata caagccctcg acgtccgtac gcccatgatc
    14701 atcaaccatt acaccaccgc aggtatagtg tgcagcaggc acaatcggta ccggttcttg
    14761 tgtgagatca atccccagcc cgagcaattt ttcataaatc atcgggaaat gctggcgaat
    14821 aaaatcggcg ggcttatggc tgatgtcgag gaacatacaa tctgcgccga ggcgtttcat
    14881 ttcatggtca atggcgcggg cgacaatatc gcgcggggcc agttcgccgc gctcatcaaa
    14941 atcgggcata aaacgcgtgc catccgggcg cttgagataa gcgccttcgc cgcgcagtgc
    15001 ttccgttaac aggaaattgc gtgcctgtgg gtgatacagt gcggtagggg ggaactgatt
    15061 aaattcgaga ttggcaacc
//
LOCUS       DAQSWQ010000105        15029 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.106, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000105 DAQSWQ010000000
VERSION     DAQSWQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.106"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(54..2006)
                     /gene="betT"
                     /locus_tag="R8G18_003311"
                     /pseudo
     CDS             complement(54..2006)
                     /gene="betT"
                     /locus_tag="R8G18_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
     gene            2145..2732
                     /gene="betI"
                     /locus_tag="R8G18_003312"
     CDS             2145..2732
                     /gene="betI"
                     /locus_tag="R8G18_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HEE9796927.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2746..4218
                     /gene="betB"
                     /locus_tag="R8G18_003313"
     CDS             2746..4218
                     /gene="betB"
                     /locus_tag="R8G18_003313"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HEE9796928.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4232..5902
                     /gene="betA"
                     /locus_tag="R8G18_003314"
     CDS             4232..5902
                     /gene="betA"
                     /locus_tag="R8G18_003314"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEE9796929.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6115..6783
                     /locus_tag="R8G18_003315"
     CDS             6115..6783
                     /locus_tag="R8G18_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796930.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYVNK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(7026..7721)
                     /locus_tag="R8G18_003316"
     CDS             complement(7026..7721)
                     /locus_tag="R8G18_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEE9796931.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7714..9141)
                     /locus_tag="R8G18_003317"
     CDS             complement(7714..9141)
                     /locus_tag="R8G18_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEE9796932.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9152..9871)
                     /locus_tag="R8G18_003318"
     CDS             complement(9152..9871)
                     /locus_tag="R8G18_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEE9796933.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10399..11253)
                     /gene="rclR"
                     /locus_tag="R8G18_003319"
     CDS             complement(10399..11253)
                     /gene="rclR"
                     /locus_tag="R8G18_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HEE9796934.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11479..12804
                     /locus_tag="R8G18_003320"
     CDS             11479..12804
                     /locus_tag="R8G18_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEE9796935.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            12913..13149
                     /locus_tag="R8G18_003321"
     CDS             12913..13149
                     /locus_tag="R8G18_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HEE9796936.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13161..13754
                     /gene="rclC"
                     /locus_tag="R8G18_003322"
     CDS             13161..13754
                     /gene="rclC"
                     /locus_tag="R8G18_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HEE9796937.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(13914..14783)
                     /locus_tag="R8G18_003323"
     CDS             complement(13914..14783)
                     /locus_tag="R8G18_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEE9796938.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         3938 a         3828 c         3594 g         3669 t
ORIGIN      
        1 gtcaatgatt cccgcgccat atgcagacat ggcgcggttt tatgcaaaaa caatcacgcg
       61 tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatgaataaa gttaaggtgc
      121 cgctcgtact ggtcaagaat atcggtgatc acctgctctt tgctgtagtc catcaggtcg
      181 ttgccctggc tgccttctaa caggaaggtt tccagtcggt agtaggtcga tttaccgata
      241 cgtgcgcggt aggtaaagcc cggcaccgaa tattgctgcg gccaaatctg atagacaaag
      301 ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgttg tccctcttcc
      361 ggcggcaggc tttttagctc cacgtacgcg ccgcgcaacc gcagctcctg cgccacttct
      421 tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt gcccggataa
      481 ttcatcaggc gcgatagacg ttttttccag ctcaggcgat cctgaagccc cagcggtcgc
      541 ggcgcggtat cgcggttgac actttcacgg cggtaatctt ctaccttcag agatttatac
      601 aaccccgcca tcacgaagaa aatcacaaag ctgaacggca gccccatgat taccgtggtg
      661 ttttgcagcg cggatatccc gttggtcatc agcatgccga gcgtcagcag accaatcgcc
      721 accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt aagctgcgag
      781 gtgaaattcc ccagcaccag cgcaccggag tccgccgagg tcacataaaa cagcaggcca
      841 gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag caggctgtag
      901 aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc gtggatgatt
      961 tcatacagcg cgctattgcc gaataccgag agccataaca gcgtgaaggt aaacggaata
     1021 atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac gcgagatacg cgccaggaac
     1081 aagccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt ccagttattc
     1141 atccactcaa ccggacggtc gaaggcaaaa ctgttgagcg tcatgcccat aaagcgattc
     1201 acatagtcgc caacgttcag caccagcgca ttaagcagga acgaggtgtc gcccataaac
     1261 aatacgaaca ggatcaatcc cagcgccagc gcaacgttaa gttccgataa cacacgaatg
     1321 cccttatcga caccggaggt gacagagatc gtggcgatta tcaccgacaa ggcgatcagt
     1381 gccgcttttg ccgccatcga atcgggaata tcaaacagta cgctcaagcc atagttaagc
     1441 tgcaccacac cgataccgag cgtagtggca ataccgaaga tagtgccgat caccgctgca
     1501 atatccactg agtgacctat cggcccgtta atccgtttac cgaagatcgg gtacagcgcc
     1561 gagcggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc catgcccatc
     1621 agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac catcgcctga
     1681 cgcgcggcct caatcgtctg tcccgcgcct tccggcggct gcatatactg cgttaccggt
     1741 tcggctacgg agaagaacat caggtcgata ccgatcccgc cagcaaacag catcgccgcc
     1801 caactcagca ggctgaattc cggtttggat tgttctggcc cgagcttcac cgaaccaaaa
     1861 cgcgaacaag cgatacagac cacaaagaca atatagagcg ttgccgccag tagatagtac
     1921 caaccgaagg ttttagaaac ccagtccagc gtgcggccaa tccacagggc cgagaagtcg
     1981 cgaaacagga tcgttgtcag ggaaaaggtc tgtcatccag tatccccact gttattgtta
     2041 ccatttaaaa tcaaattcgt aacaattacg acacatttta tattgaacgt ccaatcaata
     2101 accgctttaa tagataaaca acgctgatga atggagtggc gaaaatgccc aaattgggga
     2161 tgcagtcgat ccggcgcaga caactgatcg acgccacact ggaagcaata aatgaagtgg
     2221 gcatgcacga tgcaacgatc gcgcagatcg cccgccgtgc aggcgtttct acggggatca
     2281 tcagccacta tttcagggac aaaaatggtc tgctggaagc aaccatgcgc gatatcacca
     2341 gtcagctgcg tgacgcggtt ttgaatcgat tacatgcact tccgcagggc agtgcagagc
     2401 agcgattaca ggcgattgtt gccggaaact tcgatgaaac tcaggtgagc agtgcggcga
     2461 tgaaagcctg gctggcgttc tgggccagca gtatgcatca gccgatgctc tatcgtttac
     2521 agcaggtcag cagccgccgc ttgctgtcga atctggtgag cgagtttcgc cgcgaattgc
     2581 cgcgcgaaca ggcacaggaa gcgggctacg gccttgccgc gctgattgac ggattatggc
     2641 tgcgcgcggc actaagcggc aaaccgctgg ataaacctct cgcacactcg ctgacccgtc
     2701 actttattac tcagcattta cccaccgatt aaccgaggag acgtgatgtc ccgaatggca
     2761 gaacagcagc tttatataca tggtggttat acctccgcca ccagcggtcg caccttcgag
     2821 accattaacc cggccaacgg taacgtgctg gcgaccgtgc aggccgccgg gcgtgaggat
     2881 gtcgatcgcg ccgtgaagag tgcccaacag gggcaaaaaa tctgggcggc gatgaccgct
     2941 atggagcgct cgcgtattct gcgtcgggcc gttgatattc tgcgtgaacg caatgacgaa
     3001 ctcgcaaaac tggaaaccct cgataccgga aaagcatatt cggaaacctc aaccgtcgat
     3061 atcgttaccg gtgcggacgt gctggagtac tacgccgggc tgatcccggc gctggaaggc
     3121 agccagatcc cgttgcgtga gacgtcattt gtttataccc gccgcgaacc gctgggtgtg
     3181 gtggcgggga ttggcgcatg gaactacccg attcagattg ccctgtggaa atccgccccg
     3241 gcgctggcgg caggcaacgc aatgattttc aaaccgagcg aagtcacccc gcttaccgcg
     3301 ttaaagctgg ctgaaattta cagcgaagcg ggcctgccgg acggcgtatt taacgtgttg
     3361 ccgggggtgg gcgcggagac cgggcaatat ctgaccgagc atccgggcat tgccaaagtg
     3421 tcatttaccg gcggtgtcgc cagcggcaaa aaagtgatgg ctaactcggc ggcctcttcc
     3481 ctgaaagaag tgaccatgga actgggcggt aaatcaccgc tgatcgtttt cgatgatgcg
     3541 gatctcgatc tcgccgccga tatcgccatg atggcaaact tcttcagctc cggtcaggtg
     3601 tgtaccaatg gcacccgcgt cttcgttccg gcgaaatgca aagccgcatt tgagcagaaa
     3661 attctggcgc gcgttgagcg cattcgcgcg ggcgacgttt tcgatccgca aactaacttc
     3721 ggcccgctgg tcagcttccc gcatcgcgat aacgtgctgc gctatatcgc caaaggcaaa
     3781 gaggaaggcg cgcgcgtact gtgcggcggc gatgtactga aaggcgatgg cttcgataac
     3841 ggcgcatggg ttgcaccgac cgtgttcacc gattgccgcg acgaaatgac catcgtgcgc
     3901 gaagagatct tcggtccggt gatgtcgctt ctcacttatg aatctgagga cgaagtgatt
     3961 cgccgcgcca atgataccga ctacggcctg gcggcgggca tcgtgacggc ggacctgaac
     4021 cgcgcgcatc gcgtcattca tcagctggaa gcgggtattt gctggatcaa cacctggggc
     4081 gaatccccgg cagagatgcc cgttggcggc tacaaacact ccggcattgg tcgcgagaac
     4141 ggcgtgatga cgctccagag ttacacccag gtgaagtcca tccaggttga gatggctaaa
     4201 ttccagtcca tattctaacc aggaggttta tttgcaattt gactacatca ttattggtgc
     4261 cggctcagcc ggcaacgttc tcgctacccg tctgactgaa gatccgaata cctccgtgct
     4321 gctgcttgaa gcgggcggcc cggactatcg ctttgacttc cgcacccaga tgcccgcagc
     4381 gctggcgttc ccactacagg gcaaacgcta caactgggcc tatgaaacag agcctgaacc
     4441 gtttatgaat aaccgccgca tggagtgcgg acgcggtaaa ggcctgggtg gatcgtcgct
     4501 gatcaacggc atgtgctaca tccgtggcaa tgcgctggat ctcgataact gggcgcaaga
     4561 acccggtctg gagaactgga gttatctcga ctgcctgccc tactaccgca aggccgagac
     4621 tcgcgatgtg ggcgagaacg actaccacgg tggcgatggc ccggtgagcg tcactacctc
     4681 caaacccggc gtcaatccgc tgtttgaagc gatgattgaa gcgggcgtgc aggcgggcta
     4741 cccgcgcacg gacgatctca acggttatca gcaggaaggt tttggtccga tggatcgcac
     4801 cgtcacgccg cagggccgtc gcgccagtac cgcgcgcggt tatctcgatc aggccaaatc
     4861 gcgccctaac ctgaccattc gtactcacgc tatgaccgat cacatcattt ttgacggcaa
     4921 acgcgcggtg ggcgtcgaat ggctggaagg cgacagcacc atcccaaccc gcgcaacggc
     4981 caacaaagaa gtgctgttat gtgcaggcgc gattgcctca ccgcagatcc tgcaacgctc
     5041 cggcgtcggc aacgctgaac tgctggcgga gtttgatatt ccgctggtgc atgaattacc
     5101 cggcgtcggc gaaaatcttc aggatcatct ggagatgtat ctgcaatatg agtgcaaaga
     5161 accggtttcc ctctaccctg ccctgcagtg gtggaaccag ccgaaaatcg gtgcggagtg
     5221 gctgtttggc ggcactggcg ttggtgccag caaccacttt gaagcaggcg gatttattcg
     5281 cagccgagag gaatttgcgt ggccgaatat tcagtaccat ttcctgccag tagcgattaa
     5341 ctataacggc tcgaatgcgg tgaaggagca cggtttccag tgccacgtcg gctcaatgcg
     5401 ctcgccaagc cgtgggcatg tgcggattaa atcccgcgac ccgcaccagc atccggcaat
     5461 tctgtttaac tacatgtcac acgagcagga ctggcaggag ttccgcgacg caattcgcat
     5521 cacccgggag atcatgcatc aaccgacgct ggatcagtat cgtggccgcg aaatcagccc
     5581 cggcacggaa tgtcagacgg atgagcagct cgatgagttc gtgcgtaatc acgccgaaac
     5641 cgccttccat ccgtgcggta cctgcaaaat gggttacgac gagatgtccg tggttgacgg
     5701 cgaaggccgc gtgcatgggc tggaaggcct gcgcgtggtg gatgcgtcaa ttatgccgca
     5761 gattatcacc gggaatttga acgccacgac aattatgatt ggcgagaaaa tagcggatat
     5821 gattcgtgga caggaagcgc tgccgaggag cacggcggga tattttgtgg caaatgggat
     5881 gccggtgaga gcgaaaaaat gagtcgtgat gtgaactaac gcaggaaccg atcagattga
     5941 gagttaccgt tccagagagg gggaccgaat ccttatataa acactgaggt aactctcatg
     6001 cttcatatcc agtattcatg atgctggttt tttgctgata tttaccccga acattcctga
     6061 tacagactat taatacaacc ttatctgcgg cagattttat tttctggata tattatgagt
     6121 gaacaaatca aacaggatat cgatctgatt gagattttat tttatctgaa gaaaaagatt
     6181 cgtgttatcc tttttattat agctatctgt atggcaatgg tgcttttatt tctgtatgtc
     6241 aataaagata atataaaagt gagttacagc ctaaaaataa accagacaac accaggcata
     6301 cttgttaact gtgatagcaa taataacttt gcctgtcaga ccacaatgac tgaagatgtt
     6361 atacagcgaa ttactacatt ttttcacacc agcccagatg tcaagaacag agaaataaag
     6421 ctggaatggt caggagataa gagagattta ccaactgctg aagcggaaat atctcgcgtg
     6481 caggcgtcta ttatcaaatg gtatgcgtca gaataccata atggcaggca agttctcgat
     6541 gagatacaaa taccttcagc aattaacagt gagctttata caaaaatgat atacctgacc
     6601 aggaactggt cattgtatcc gaacggtgat ggctgtgtaa ctataagctc accagaaata
     6661 aaaaataaat accctgctgc catttgcctg gctctgggat tttttctaag cattgtaatt
     6721 tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat accaacaaaa ctctgggcag
     6781 taataaacag cattctgata gagagtgcct gtataaagct gaagtgacaa tgaccgggaa
     6841 gggaacccgt tttcaggaat ggatgcctgt ctttttcact gtaaatatcc tggcattact
     6901 aatgccagga tatttacaga agagcagcat aaccatgacg gaacggggtt aagtcaggtt
     6961 gtccttaacg ctattttcaa cctcgtatgc cttcttcagg tttatgttca gatctcatat
     7021 ctctctcagc aatcctcaat aatcagatac accgctttca ccggaccatg aaccccgacg
     7081 actttgataa gctcaatatc cgccgttgag ctgggaccac tgatgatgtt aatacatgac
     7141 ggcattcgtt caccggcctg cgctttctga tgcaattttt ctgcgagttg cgctacacgc
     7201 ggcaggattg tgcttttacg caggataaaa agagaagatt ccgggagcag gctcaatgaa
     7261 cgcccgcgct cgacggcgga aaaaagaacc acgcctcccg attcggttaa accatattca
     7321 gcatacacga cacccacttt agcctgctct gcctgcgaga tattctcggc acctttcgcc
     7381 ggatcccaaa caacggcatt gcattcctgc tgcaaacgtt cactaatccc caattcctcc
     7441 agcctcgtgt caccgctaat cacgaccgac tgatctccca gctctttaca cagacgtatt
     7501 gcagcttctg ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat aacatcgctg
     7561 gcaaactgaa taaacgcgtc acagcgctgc tgttggttaa gttgggtaag ccgctcgtta
     7621 gcatagttgt taagcggcgc atcttctgct tgcggttcaa gtcgcagcgg gcgacccagt
     7681 gcctgagcaa cgttattcaa aaattcgctc cgattatcca ttctttttct cctgcgcctg
     7741 atgtttctta aaccaactac ggaaactctc tccgtcagct tcaggaagat cgcgtgcttc
     7801 catccagtcg ctaatcgcgc caaatttgag tggtgttttg ccgccattga taaaccagct
     7861 tgccgcatga gcgccggcca tcatcccgac tttccacaat cctggatgac tattggcata
     7921 agcgaacatt tttatcgccc gttgctctgc ttttgcggtg atcccttttt cagccatcac
     7981 ccgacgatga cgcaaaatca gttttgacag cggaatacgc accggacaca cgctgtcaca
     8041 agcagtgcat aaagagcagg cgtagggtaa atctttaaaa tctttatagc cgccaagtag
     8101 cggagaaatc accgcaccaa ttggccctgg ataaatagag ccatatccat gaccgccaat
     8161 atggcgatat gccggacaag tattcataca agccccgcag cgaatacagc gcagcacatc
     8221 ccgaaattca gaggccagca cctcagaacg cccgttatcg acaataacca gatgaaactc
     8281 ttcaggacca tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt
     8341 caaacgtgca ccaacggcac tgcgcgccag catggtgatc aatacatcta cctcggcaaa
     8401 cgtgggggca atacgctcca ttcccatcac tgcaatatgc gttttaggca gcgtggtaca
     8461 cattcgcgca ttaccttcat tggtcactag gcataccgaa cctgtctctg ccaccgcgaa
     8521 attacagccg gtaataccta tttcagcact gaggaaatct tcgcggattt tttgccggat
     8581 gaataaggtc atcgcttcag gcgtttccgg cccctcatag cccagacgtt cgtgtagcac
     8641 tcgacggatc tgatggcgat ctttatgaat tgccgggacc acaacatgag atggcggatc
     8701 ttgatccagc tggagaatat attcacccag atcggtttca atcacctgaa tgccagcatc
     8761 ctgcaacaca tgattgacac caatctcttc ggtcaccatc gatttagatt tcaccacctt
     8821 ccgggcattt ttgcgttggg caacctgtaa aatgtagcgg gtagcgtctt ctttggtttt
     8881 tgcaaaccag acgtgaccgc cgttttgcgt cactttttct gagagctggt acagataagc
     8941 gtcgagatta ctcagaacat gatcacgtat ctgggcggcc cgatcgcgcc actcctccca
     9001 gtgccccaat tcatcgacca ttttttgccg atttgcccca atacgctgct gcgcgtttgc
     9061 caccgctttg cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt
     9121 attactggtt ttgatcgaca tctttatatc ctcagcggct catcaacact tcagcaatat
     9181 gcatcacttt gactttctgc ccttcccgtt gtaatcgccc actgatgttc agcaggcaac
     9241 tcacgtcagc accaattaaa tactcagggc ggacttccat caggtgcaca accttttctt
     9301 tcaccatctc gccggatatt tcggccattt tgaccgagaa cgtgccgcca aatccgcagc
     9361 aggtatcctg ttcagcaaag gtcaacagct ccagtccacg cacatttttc agcagcgtaa
     9421 gtggctcgtc cttcactccc agcttacggg ccaggctaca agatgggtga tacacagctc
     9481 tcccttgcaa actggcacct acatcgacta cccctaattt attaacaata aaagaggtga
     9541 gatcctgcat acgcgcggca accttttcgg cacgtgatgc ccattcgggt tcatccgcca
     9601 gatacgtcgg gtaacttttt acggcatagg tgcaagagcc agccggtgaa ataatgggat
     9661 cgtcgttatc ctccagtgcg gcgatcagat ttttcatccc tggaatcgct tctttgatat
     9721 aaccgctatt gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa tttacgcgac
     9781 agccgagttt ttccagtagc agcacggagt ctcgtgccat tcttgatttc agggcgtcac
     9841 caatacaggt gacaaagaaa ttgacattca caactaatac tccattactt catgcccatt
     9901 tatgcccgat ggttcccctc cttccggggg atcaatcatc aataaatatg gcgttctttt
     9961 ttaattggct agcaacatac cacccctcat ttgtgtgaca aatgataatc tttcatccaa
    10021 acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt atggggaaat
    10081 cgacgttcat ttttggcata agaaatccat ttaaaaaggc tacatccctt atacctattc
    10141 agagctactt attcaggggt tattgagata tacactgatg gtgtacaaaa aaatcaccga
    10201 caataatcac gtatcaagta atagtcgggg ttttatttaa ttgaagcaat ttagtttgca
    10261 tttttattta aaaactgagc tagagtccat cagaaaaaaa tatataaagc ccaaaccacc
    10321 gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta tctttgccct
    10381 gatttctggc ggttttattc agggtgcaag ctgtctgacc ctttcccgat attctcccgg
    10441 agtacaacca aactcgcgga caaacgcctt gtgaaaagat gattcactgg catagcctac
    10501 tgactcagcg atcaccacaa cagggagcat ttcccgggaa aacatctggg ccgctatttg
    10561 tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac ggaaaagctg
    10621 ggcaaaactt gcccgagaca tgtgggcgat gctggccagc gattcgacgg tccaggcgtg
    10681 tcctggcatt tccagcattt gctgtattac cgcaccaaga cgtggatgca gaagcaaact
    10741 gagaatgttt ttctctgtat taacctgtgc aatccactca cgcaccgcga gggtaaagaa
    10801 tgtagcgcag atctggctac acagtgcatc cactcccggc atcgccgatc tggattcctg
    10861 ttgtaaaaac gggatcgcct ccctcagcca gttatattcc acgctgtggt taaccggtgc
    10921 taaaaacagc gtttccggca aagacgttaa aaaataacgc gccgaatgtt gcaaccgaag
    10981 agtgccgcag acaatacagg tcgattcatt atcgacatga ctcaggcgat gagctgaatt
    11041 ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctctcccg tcggcatttc
    11101 cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata accgataatt ccccggcacc
    11161 atgttgaagc tgccagtcac ttcctaacac gcaattctta tcgatcgttc cttgtggagc
    11221 gttaagcatc aacaaacggc taagggcatc catatcagac tcctgagtgg tttttgatta
    11281 caaacaccaa caatcttagt aaataactaa tctaatacca tcaaatggtt aatcatgatt
    11341 tttgttttgt gattctgatt aagaaaaaga aaataccaga cgataagaca aaatatcaag
    11401 cgttgtgaag aaatggtatt tgctcttttg cgtctatagt catgatgtca aatgaacgtg
    11461 tttcgacagg aaatcatcat gaataaatat caggcagtta ttattggttt tggcaaggct
    11521 ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gagtggctct catcgaacaa
    11581 tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gcatcccaac caaaacattg
    11641 gttcatgacg cacagcagca cacagatttt gtccgtgcca tacagcgtaa aaatgaagtg
    11701 gttaattttt tacgtaataa gaattttcat aatcttgcgg atatgcccaa tatcgacgtg
    11761 atcgacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg gcctgaggga
    11821 aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac cgtggttccg
    11881 ccaattcctg gaattaccac cacgcctggt gtatatgaca gcaccggatt acttaatcta
    11941 aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt tgagttcgcc
    12001 tctatgttcg ctaattttgg cagcaaggta accattttag aagcagcttc gctgtttttg
    12061 cctcgggaag atcgggatat tgctgataat atcgcgacga ttttacgcga tcagggcgtc
    12121 gatattatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca agtgcaagtg
    12181 catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg tcgtcaaccg
    12241 gctaccgctt cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg cggggcaatt
    12301 gtcgttgaca agcaattaca taccaccgca gacaatattt gggcgatggg agatgttacc
    12361 ggcgggctgc aatttactta tatttcactg gatgattacc gcattgtgcg tgatgagttg
    12421 ctgggagaag gcagacgtag tactgatgat cggaaaaatg tgccttattc cgtattcatg
    12481 acaccgccac tgtccagggt tggtatgaca gaagaacagg ccagagagag tggtgctgat
    12541 attcaggtgg tgacattgcc tgtagctgca attccgcgtg ccagagtgat gaatgatact
    12601 cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatattagg ggcatcactg
    12661 ctgtgtgttg actcccacga gatgatcaat atagtgaaaa tggtgatgga tgccgggctg
    12721 ccttatagca tattacgcga tcagatattt actcatccgt cgatgagcga atcactcaat
    12781 gatctatttt cattagtcaa ataaactcaa aatcagacgc cagaacaaat attctggcgt
    12841 ctcagagaaa agaatcttat taattctctg ttactctata acttcttaat cacttcattg
    12901 atggtatttt atatgtttaa aaaatctgtt ttatttgcaa cactattatc tggcgttatg
    12961 gaattttcca ccaatgcaga tgataaaata attctgaaac atatcagcgt ctcgtcagta
    13021 tcagcatcac cgacagttct ggaggatacc attgctgata tagccagaaa atataatgct
    13081 tcatcctgga aagtcacatc gatgcgaatt gataataatt caaccgcaac agcagtattg
    13141 tataaataag gatgttcaca atggaaaaat acctgcacct gttaagtcgg ggagataaaa
    13201 ttggcctgtc attgattcgt ctgagtattg cgattgtttt tatgtggatt gggttattaa
    13261 agtttgtccc ttacgaggca gacagcatta caccattcgt cgcaaacagt ccactaatgt
    13321 cgttctttta tgaacacccg gaagactata aacagtatct gactcacgaa ggcgaataca
    13381 aaccagaagc aagggcatgg caatcggcca ataataccta tggtttttcc aacggtcttg
    13441 gcgtcgtgga ggtgattatt gctctgctgg ttttggctaa tcctgtcaat cgctggttag
    13501 gtttattggg aggactgatg gcatatacca cgccgctggt aacactctca tttttaatca
    13561 ccaccccgga ggcatgggta cccgcattgg gtgacgctca tcatggtttc ccttatttat
    13621 ccggtgctgg tcgcttggta ttgaaagata ctctgatgct ggcaggtgct gtcatgataa
    13681 tggcagattc ggcgcgggaa attcttaaac aacgcagtaa tgaatccagt tcaacgttaa
    13741 aaactgaata ttgataatcc gaacactgtc tgttgttcac tttttcaggc gtcaggcccc
    13801 ctttctgatg atgaagcctg acgccatatt accggagata tgacagagtg aagatcaata
    13861 tcactcccct gttaaaagat tcttgtcgtc tcaggtaaaa tatatgatta atatcaatca
    13921 tgtattttta cagcaagaca accacgaaca aattcaggat taaaatgttt taccgactcc
    13981 ccaacataac ccaaatcaag cgaactaatt tgtgccattt ctttctctgt cagtgagaaa
    14041 tcccagatag caaaattttc ttcgatacgc tgttgatgtg tcgatttcgg aataacgacc
    14101 actccccgtt gaatattcca acgcagaatg acttgagaaa tcgatttttg atgggcatct
    14161 gcaatactct gaagcagatt attttcaaag ggtttatgtc gtccaccacc taacggagcc
    14221 catgcttcag gctgcacgtt ataatacttc atggttgcca gcgcctctgg ttgggcaaaa
    14281 tagggatgca actcgacctg gttgaccatc ggtttaactc ttaccgtttc acaaaagttc
    14341 gccagaacat gaggatagaa attggaaacc ccaattgctt ttaatttgcc ctcttcatag
    14401 gcatcttcga gtgcacgcca cacactgaaa taatcgcgca tagcctggtg caataaatag
    14461 aggtcgaagt actctagtcc cgattttttt aatgatgctt caatacctgc tgctgccgta
    14521 tcctgattca acatatcctg cccccacaat ttagacgtta taaataactc atctcgggta
    14581 cataaacctt cagatatagc ttcacgaacg gcttcaccga cggcatcttc attaccgtat
    14641 accgctgcag tgtcgatgag tcgataaccc gtgcggatag cgttcaacac tgactgtttg
    14701 cacacgtttt tatcggtaac ctgaaaaaca ccaaatccca tcatcggcat tttcagattg
    14761 ttacttaata cggaaaattc cactgtcagt ccctccatta ggcatgaaca atgagtctac
    14821 gttaaaacat aacctcaaat tagtatgtgg attttgatat cacttatgca aaaaattcat
    14881 taataatgta ggactgaaac ctctctattt tcggggacaa cgaagcagac gctaccagtg
    14941 cttttgcctt cgaccttgct gtttttgata cacttagggc cctgggcata acgaaaatgt
    15001 gcgatatcac gaagaattaa tggagaatg
//
LOCUS       DAQSWQ010000106        14619 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.107, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000106 DAQSWQ010000000
VERSION     DAQSWQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.107"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            71..1606
                     /gene="lsrA"
                     /locus_tag="R8G18_003324"
                     /pseudo
     CDS             71..1606
                     /gene="lsrA"
                     /locus_tag="R8G18_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1600..1908
                     /locus_tag="R8G18_003325"
     CDS             1600..1908
                     /locus_tag="R8G18_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9796939.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2080..3528)
                     /gene="uxaB"
                     /locus_tag="R8G18_003326"
     CDS             complement(2080..3528)
                     /gene="uxaB"
                     /locus_tag="R8G18_003326"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEE9796940.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3755..4705)
                     /locus_tag="R8G18_003327"
     CDS             complement(3755..4705)
                     /locus_tag="R8G18_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEE9796941.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4844..5203)
                     /locus_tag="R8G18_003328"
     CDS             complement(4844..5203)
                     /locus_tag="R8G18_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HEE9796942.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(5203..6129)
                     /gene="glsB"
                     /locus_tag="R8G18_003329"
     CDS             complement(5203..6129)
                     /gene="glsB"
                     /locus_tag="R8G18_003329"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HEE9796943.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(6193..7581)
                     /gene="sad"
                     /locus_tag="R8G18_003330"
     CDS             complement(6193..7581)
                     /gene="sad"
                     /locus_tag="R8G18_003330"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HEE9796944.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7682..8563
                     /locus_tag="R8G18_003331"
     CDS             7682..8563
                     /locus_tag="R8G18_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9796945.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            8640..9755
                     /gene="yneK"
                     /locus_tag="R8G18_003332"
     CDS             8640..9755
                     /gene="yneK"
                     /locus_tag="R8G18_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HEE9796946.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            9905..11095
                     /gene="ydeA"
                     /locus_tag="R8G18_003333"
     CDS             9905..11095
                     /gene="ydeA"
                     /locus_tag="R8G18_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HEE9796947.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(11120..11785)
                     /gene="marC"
                     /locus_tag="R8G18_003334"
     CDS             complement(11120..11785)
                     /gene="marC"
                     /locus_tag="R8G18_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HEE9796948.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            11997..12431
                     /gene="marR"
                     /locus_tag="R8G18_003335"
     CDS             11997..12431
                     /gene="marR"
                     /locus_tag="R8G18_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HEE9796949.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            12451..12834
                     /gene="marA"
                     /locus_tag="R8G18_003336"
     CDS             12451..12834
                     /gene="marA"
                     /locus_tag="R8G18_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HEE9796950.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            12866..13084
                     /gene="marB"
                     /locus_tag="R8G18_003337"
     CDS             12866..13084
                     /gene="marB"
                     /locus_tag="R8G18_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HEE9796951.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(13141..14580)
                     /locus_tag="R8G18_003338"
     CDS             complement(13141..14580)
                     /locus_tag="R8G18_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEE9796952.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         3785 a         3745 c         3392 g         3697 t
ORIGIN      
        1 aggttatgaa caaattaaaa gcagaaatac atttgttcaa aactcacctg caaaactgaa
       61 cgggggaaat atgcaaacga gtgatacccg cgcgttaccg ctactttgcg cccgctcggt
      121 ttataaacag tattcagggg tcaatgtcct gaaaggcatc gattttacgt tgcatcaagg
      181 ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa tcgacgttaa tgaagattat
      241 tgccggtatt acccctgctg atagcggtac gctggagatt gggggcaaca actacgtcag
      301 attaacgcca gttcatgctc atcagctggg tatttatctc gttccccagg aaccgctgct
      361 tttcccaagc ctgtcgataa aagaaaacat cctgtttggg ctggcaaaaa aacagctctc
      421 catgcagaaa atgaagaact tgctggcggc gctgggctgc tagtttgatc tgcatagtct
      481 ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa atcctccgcg gcctgatgcg
      541 cgactcgcgg attctgatcc tcgatgaacc taccgcctcg cttacccctg cggaaaccga
      601 acgcttgttt cgtcgcttgc aagagctgct tgctactggc gtgggtattg tttttatctc
      661 gcataagctg ccggaaattc gccagattgc cgatcgaatt agcgtgatgc gcgacggaac
      721 catcgcctta agcggcaaaa ccagcgaact gtctaccgat gacattattc aggccatcac
      781 gccagtggta cgggaaaaat cgctctctgc cagccaaaaa ttatggctgg agttacctgg
      841 caaccgccca caacatgccg ccggaacgtc ggtgctgaca ctagaaaatc tgaccggcga
      901 aggtttcagg aatgtcagcc tgacgctcaa tgccggagaa attctgggtc tggccgggct
      961 ggtgggggcc ggacgcacag aactggccga gacgctctat ggtctgcgta ctttgcgtgg
     1021 cggacgcatt atgctgaatg gtaaagagat caataaatta tccactggag aacgtttact
     1081 gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc ggactgaatc tcgatgcttc
     1141 gctggcctgg aacgtctgcg cccttactca taaccttcgt ggattctggg cgaaaaccgc
     1201 gaaagataat gccaccctgg aacgttatcg tcgggcgctg aatattaaat ttaaccaacc
     1261 ggaacaagct gcacggacat tatccggtgg caaccagcaa aaaatcctga ttgccaaatg
     1321 cctggaagct tcgccgcaag cattgattgt cgatgagccg acgcgcggcg tggatgtctc
     1381 ggcccgtaat gatatctacc agctgttgcg cagcatcgcc gcacagaatg tggcagtgct
     1441 gcttatctcc tctgatctgg aagagatcga actgatggca gatcgtgtgt atgtgatgca
     1501 tcagggcgaa attacccact ctgcactgac cgggcgcgat attaatgtcg agactattat
     1561 gcgcgttgcc ttcggcgata gtcagcgtca ggaggcgtca tgctgaagtt tattcagaac
     1621 aaccgtgaaa tcacggcact gctggcggtg gtgctgctgt ttgtattacc cggttttctc
     1681 gaccgccagt atttaagtgt gcaaacgctg accatggttt atagcagcgc gcaaatcctg
     1741 atcctgctgg caatgggcgc gacgctggta atgcttatgc gcaaagtagt aagcgcgatg
     1801 atatcttgga taatactgag ccgtgccaga caaaaatacg gcgcagccag tgttgttgtg
     1861 gacgaacaat catggttatc ttcaggcgtg aaaaagtcgc cctattaaac gtgattacga
     1921 tcacattctc aagacgctac ttacaaatta cctacaagct tacaacatta acaggcgctg
     1981 catgtggcat ctgttaatga taaaggcaca taggatgttg taactaatat agtgaaataa
     2041 gaaacccggt caaaaccggg ttcagaagta acggtgttat tagcacaacg gacgtacagc
     2101 ttcgcgcatc cctttttcaa gaatcgcatc caggtcatta gcaacctgct cgaccagacc
     2161 aggcacttgc gtcaggtcct gctcccagtg gtctttttct gccaacacaa tcgctaccag
     2221 ttcctgggta ccgataacat ggtcacgatg ctggctccag agttgctgat aacgttccag
     2281 ccagtgtgca tcgtcctgta ccggatatgt ttctccgtta cgctcaccac gatagaacgc
     2341 aatcaatgcc gctaatgcga aagtaaggcg cgccggaagt gtgccgtttg ccttctgccc
     2401 tgccagcagc tgcggcagga tgcgggtgcg gaacttggtc ataccgttga gcgcgataga
     2461 cagcagctga tgcttatacg ggttacggaa acgcccggtg actgcactgg cgaaagattc
     2521 cagttcatca cgaggcaaat ccagtaccgg aataatttct tcgtagatgg ctttttcaac
     2581 gaatgcgcaa atttcagcat cgttcatcgc ttcacctacg gtatccagcc ctgcctggaa
     2641 cgccaccggc accagcgcgg tgtgcgcacc gttgaggatt gccactttgc gttctttata
     2701 cggtttaata tcgtcaacaa tcagcacgtt gagcggatat ttgtccaggc gcagctcagt
     2761 cgctaaggat ttcggcccct gaatcacaaa caggtaaaag tgttcagcgg tgtcgaggaa
     2821 accatcgtga taacccagtt cttcttccag tttagccact tcatcgcgcg gataaccggt
     2881 aacgatacgg tccaccagcg tagaacagaa gctgttagcc tgatccagcc attgaataaa
     2941 tgcttctggc agtgcccact cttgcgcata gcgcagcacc agttcacgca acgcgtcgcc
     3001 attgtagtcg atcaactcac acgggatgat gatccaacct ttatccagcg caccgttgaa
     3061 atggctaaag cgttcgaaca gcagacgggt cagttttgcc ggatagctta tcgctggcgc
     3121 gtcatcgaat ttatcgcccg catggtagct gatccccgct tcggtggtgt tagagaaaac
     3181 aaagcgcatt tccgggttgt gcgccagttt caggaattca tcgtattcac tgtagacgct
     3241 gatttcacga ttaaccgagc gaatcagacg cgcgtcgctg accgcttccc ccttctcatt
     3301 caggccacgg ataatggtgg tatacagacc atcctgcgtg ctcagtgacg gcgggaatga
     3361 agtttcaatc ggacgaacaa cgaccacgcc agaattcaga tcggtgtgct cattcaggag
     3421 atcgatttgc cagtcaacaa aggcgcgcag gaagttacct tcaccaaact gaatgatacg
     3481 ttctggatac tgtgcaccgg gaaaatcgcg acgatttagt gttttcacaa tgggttccct
     3541 tctgattagt catacaacct gtttgaattg gtacgacagg ttagcaaact ttaatacgcc
     3601 gaacccctgt tttgatcaac tcctgatgat taatgagcag ttttatgaga aaagtgtggc
     3661 gcggatcatg gtttaatcga ggaaatacgc cttttcctgg atcataaagt ggtagaacac
     3721 attgcattca aatcgcgcgg aatgaataaa gatgtcagac aacttcctca ccgtaacgca
     3781 tagtgctggt acggttgcgc ccatctttct tcgagcgata cagacacgta tcggcttcga
     3841 ccatcaattt attaaaatca tcggtcagcg ttcggtacga tgcgcgacca ctaccgacgc
     3901 caatacttac cgtgagataa agcgtttttt gttgccaggt gaatggttgc agttcaacgc
     3961 ctttacggat tttttccgcc attagcagac catcgacagg attcaccgac ggcactgcaa
     4021 cagcaaattc ttcgccgccc atgcgcgcca ccagcccctt atcaccgaca atcttctgaa
     4081 tatgccgggc aaacacgctt aacactttat cgccacattc atgtccatag ttatcgttaa
     4141 tgcttttgaa atagtcgata tcaagcagca tgaccgtcag aggcacgatc aacacgcccg
     4201 ttgagaattg ctcactgaac cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac
     4261 tgaccgaacc aatcgccccg acaatagcgc ataataatgc ggcaatcaga cagtaattaa
     4321 atagccgtaa ggcacttact ggctcatact tatttttccc cagacgctta tcccgcacga
     4381 ccagtaaggc gacggtaaca ataaacatca tattggagaa attgataacc agtgaaacca
     4441 gtccccattc ggtggttatg gcatcataaa ccagcatcgc cacataactg atcgcatagt
     4501 aatgcagtcg attaagccag acatagcggg caaacccccc cgccattacg ccattcagcg
     4561 gccagaaaag agaaagttcg tgaaccagcc gtaactctgc accaataaaa tagaacagcg
     4621 tggttagcgc aaaaatagcg atgctattac gtagcagatg accttcctgg aacaaacgga
     4681 aagttgaaat ggggtgtaca tgcattaatt atctgtccat acaattgcgg gtggcaatta
     4741 tctcaacgta ataagtaaat tctcataaag gatagcatgt tcgctaacag cggcggcaaa
     4801 taaaggtacc ctgggaatgc tattttatcg ttttccgcaa ttctcatggc tgattcatct
     4861 gtacaaccag ccagtgatag attaccgcca cgatataacg ttgttgttct tcgcttaacg
     4921 aaacaccctg cggaatgtta tgccacttag ccagacaacc gcgacagcag gtcgcggtgg
     4981 cgtgctgagc gataaacacc ggatgcccgc gcatgggggt ttgcttaccg tcattagccg
     5041 gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc atgttgttcg atgactggcg
     5101 cgcctttctc caggcaatac tgacgctctt tcatgcccag acgaaagcga gagcgaaatt
     5161 ttgaacggga taaacgcgcg aagagtggat cgagagactg cattaataaa ccgaacgccc
     5221 taactgtttc gtcaattgtt caaggacggc gatacccgca agcgagttac ctgcatcatc
     5281 cagttccgga ctccagactg cgatggccat ttcatgcgga acaatcgcca caataccgcc
     5341 accaacgcca gatttcgccg gtagtcccac ccgccaggca aactcccccg cgttctggta
     5401 cataccactg gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcactacagg
     5461 ttcatcaata tgaatagctt tcccctgatt agccagaaag acaaacgtcc gagccagctc
     5521 tacacagctc attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc
     5581 atgatggaaa ttgccaaacg acttcatcag ccaggcgata gccgcatttc gcgcggaatg
     5641 ttcaaattcg gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc
     5701 gcgcacgact tccagcatac gttgccgtgg tgcgcttaat cgcccttgca acatatcgca
     5761 gaccaccagc gcaccagcat taataaacgg attacgcgga ataccctgtt ccatttccag
     5821 ttgcactaaa gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc
     5881 ctcttcggag taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat
     5941 ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc
     6001 aatgcccaat cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc
     6061 ctgaccaatg agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc
     6121 gactgccact ttctactcct ggaccgcagg tctgaaaaga cctgcgagta tatcagagct
     6181 gaatatgtcg cgtcagatcc ggtctttcca caccgtctgg atattacaga attcgtgtaa
     6241 gccgaaatgg gaaagctcac gaccaaagcc actctttttc acgccaccaa aggccactcg
     6301 cgcgtcgctg gcacaataac cattgataaa caccccaccg cattccagac gtgccgccat
     6361 ctgtctggcc tgtgtttcgt cggtggtaaa aatggtcgct gaaaggccga actcactatc
     6421 attagccagt tccagcgcat gttctgcatc tttcgcaacg gtgattgccg caacggggcc
     6481 aaacatttct tcccgaaacg cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg
     6541 atagtagtta cctgccccag ccatcttttc cccgcccagt aacaaacgcg caccctgcgc
     6601 cagggttttc tctacctgat gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc
     6661 gagagtgttc tcttcgtcac ggggatcgcc cattttcaag gctgccgcag ctgccacaaa
     6721 acgttcggta aatgcagaag caattccctc ttcgataata aagcgttttg ccgctgcaca
     6781 tacctgtccg gtattctgat aacgtccggc taccgccgct ttaaccgcca gttccagatc
     6841 ggcatcgtta agcacaataa acggatccga accgcccagt tccagtacgc attttttcag
     6901 tgccgctcca gcctgtgcgc caatagccgc tccagcacga acacttccgg tcaccgtgac
     6961 agcagcaatg cgcgagtctt taatcatctg actgacaccg tcgttgtcgg cattcagcca
     7021 gccatatacg ccttgcggga ttcccgcatc tttaaacacc tgggcaatga gctgagcaca
     7081 tcccatcaca ttcggcgcat gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac
     7141 agcgccacgc atcacctgcc ataacggaaa attccacggc ataatcgcca gaatcgtccc
     7201 caacggtcga tactcaataa ccgcctgctg attttccacc agtgtaggtt ccgccttcag
     7261 cattgccgga ccatgttctg cataccagtc acacaaattc gccgatttcg ccacttcagc
     7321 gcgcgcctgg ttgattggtt tgcccatttc gcgggtgatc atttgcgcca tttcttcgct
     7381 acgggcgcgc agagccttac cgatatcacg cattttttca gcacgataat ctatatttgt
     7441 ctcgcgccag tcgcgaaagc ctgctgccgc cagctcaagt gcgttttcga tatcgttagc
     7501 gccagcccac ggcagcacag aaagttgttc acccgtggca ggatttattg aaattgcatg
     7561 agttgccgga gtaatggtca tcggggtatc tcctttatga gtcatggtat gaagatacgc
     7621 agatttactc ttgctttaaa atgaataata ttaagccact tattcacgaa tcgagaatgt
     7681 tatggatctg acccaactgg agatgttcaa cgcggttgcc gaagctggca gcattaccca
     7741 ggctgcagca aaagtgcatc gcgtaccgtc gaatctcact acccgtctac gccagctgga
     7801 aacagaactg ggggtagatc tgtttattcg cgagaatcag cgtttacgtc tctctcctgc
     7861 cgggcataac tttttacgct atagccaaca aattctcgcg ttagtggatg aagcgcggag
     7921 cgttgtcgct ggcgatgagc cgcaaggttt attttctctt ggttcgctgg aaagcaccgc
     7981 agcagtgcgc attccggcca cgctggcgga attcaaccat cgttatccca aaattcagtt
     8041 ttcactttcc accggccctt ccggcactat gctggaagga gtgctggagg gaaaactgaa
     8101 tgcggcattt attgatgggc ccattaacca tactgccatt gacgggatac cggtataccg
     8161 cgaggagctg atgatcgtca cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt
     8221 taacggcagt aacatttatg ccttccgcac caattgttcg tatcgtcgcc acttcgagag
     8281 ctggtttcat gctgacggtg ccgctccggg aactattcac gagatggagt cgtatcacgg
     8341 gatgttggcc tgtgtggtcg caggagcagg cattgcgctt attccacgct ctatgctgga
     8401 aagtatgccg gggcatcacc aggttgaagc gtggccgtta gctgagcaat ggcgttggtt
     8461 aacaacctgg ctggtctggc gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca
     8521 actactggat gtgtctgact cggcaaaaca gagttaccaa tgaactattt tgatagttct
     8581 tgcggctaat atgctctata tagtgatgtt ccgatgactt atgactatat ggggcaaata
     8641 tggttacgcc agtaagcatc agcaattaca tatctcttcc tgatgatttt cccgttcgta
     8701 atattgcacc acaggtaaaa gaagttttaa aggactttat tgatgcgcta agtacaataa
     8761 tatgtgatga agagtggcgc acgtctttaa acatcaactc agccacaaaa aagatattta
     8821 ataaccttga taacttatca tatattcaga ggacttcttt ccggggtaac gacacgctat
     8881 acaatgaaaa ggttcagttt aaactcactt atcccgtcaa gaatggaaga cacaaagaaa
     8941 atattgaatt tcaggtagta ataaatttaa gccctattta tttagataat tttcgccatg
     9001 atggagaaat taatattttt tgcgctccca acccaaagcc tgtcactatg gggcgcgtat
     9061 ttcagaccgg cgtcgagcgt gtgctctttc tttttctgaa tgattttatt gaacaatttc
     9121 caatgttcaa ccctggcgtt ccgatcaaaa tagcgcatac accacatatt gaacccctgc
     9181 ctccggatca ccataccgct gcagattact tacgccagtt tgatttgctt gtcctgaatt
     9241 ttatctctcg cggtaatttt gtcatactcc cccgattatg gaataactct gaggttcaca
     9301 gatggttcgt caataaagat cctaacttga tcaccgccat tctcgacata acggacagcg
     9361 aattaaaaga ggatttattg caaagcctga tggattcatt aggttctaac aaacatgtac
     9421 tacccgaagt ctgcatctgc tttttattcc ttttagcaga acaagaatct cctcattttc
     9481 aggacttgtt tttatttttc gccaatatgt tattgcacta tcaccaattt atgaatccca
     9541 atgaaagtga tttgaatgac gtgttgatgc cagcatcatt aagtgatgat aaaattatca
     9601 agcatatggc gcgcaggacc ctcgaactgt ttgtaaaaaa tgaaacaccg ccaaaagtta
     9661 ctcacgaaga cctggtgaaa aacaggcctc gctcccctgt cagaccacct atacccgcaa
     9721 cctccaaaac gccagacctc cctgaacgtc attaaaccgt gatgttaccg actctctgac
     9781 gcggaacaga atcagcgtca gagaaacgga aaacgcgatc cagatcacaa atacattgta
     9841 ttcacatcat taaccgtttt aagatcattt catcactttt tcgcaactca cccgataatc
     9901 tgttatgaca acaaacactg tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc
     9961 agtcgccgcc ttcatcttca acaccaccga atttgtccct gttggcctgc tctctgacat
    10021 tgcgcaaagt tttcacatgc aaaccgctca ggtcggcatc atgttgacca tttacgcatg
    10081 ggtagtagcg ctaatgtcat tgccttttat gttaatgacc agtcaggttg aacggcgcaa
    10141 attactgatc tgcctgtttg tggtgtttat tgccagccac gtactgtcgt ttttatcgtg
    10201 gagctttacc gttctggtga tcagtcgcat tggtgtggct tttgcacatg cgattttctg
    10261 gtcgattacg gcgtctctgg cgatccgtat ggctccggcc gggaagcggg cacaggcctt
    10321 gagtttaatt gccaccggta cagcactggc gatggtctta ggtttacctc tcgggcgcat
    10381 tgtggggcag tatttcggtt ggcgaatgac cttcttcgcg attggtattg gggcgcttat
    10441 cacccttttg tgcctgatta agttacttcc cttactgccc agtgagcatt ccggttcatt
    10501 gaaaagcctc ccgctattgt tccgccgccc ggcattgatg agcatttatt tgttaactgt
    10561 ggtggttgtc accgcccatt acacggcata cagctatatc gagccttttg tgcaaaacat
    10621 tgcgggattc agcgccaact ttgccacggc attactgtta ttactcggtg gtgcgggcat
    10681 tattggcagc gtgattttcg gtaaactggg taatcagtat gcgtctgcgt tggtgagtac
    10741 ggcgattgcg ctgttgctgg tgtgtctggc actgctgcta cctgcggcga acagtgaaat
    10801 acacctcggg gtgctgagta ttttctgggg gatcgcgatg atgatcatcg ggcttggtat
    10861 gcaggttaaa gtgctggcgc tggcaccaga tgctaccgac gtcgcgatgg cgctattctc
    10921 cggcatattt aatattggaa tcggggcggg tgcgttggta ggtaatcagg tgagtttgca
    10981 ctggtcaatg tcgatgattg gttatgtggg cgcggtgcct gcttttgccg cgttaatatg
    11041 gtcaatcatt atatttcgcc gctggccagt gacactcgaa gaacagacgc aatagttgaa
    11101 aggcccattc gggccttttt taatggtacg ttttaatgat ttccaggatg ccgttaataa
    11161 taaactgcac acccatacat accagcagga atcccatcag acgggagatc gcttcaatgc
    11221 cacccttgcc caccagccgc ataattgcgc cggagctgcg taggcttccc cacaaaataa
    11281 ccgccaccag gaaaaagatc agcggcggcg caaccatcag tacccaatca gcgaaggttg
    11341 aactctgacg cactgtggac gccgagctaa taatcatcgc tatggttccc ggaccagcgg
    11401 tacttggcat tgctagcggc acaaaggcaa tattggcact gggttcatct tccagctctt
    11461 ccgacttgct tttcgcctcc ggtgaatcaa tcgctttctg ttgcggaaag agcatccgaa
    11521 aaccgataaa cgcgacgatt aagccgcctg caattcgcag accgggaatc gaaatgccaa
    11581 atgtatccat caccagttgc ccggcgtaat acgccaccat catgatggca aatacgtaca
    11641 ccgaggccat caacgactga cgattacgtt cggcactgtt catgttgcct gccaggccaa
    11701 gaaatagcgc gacagttgtt aatgggttag ctaacggcag caacaccacc agccccaggc
    11761 caattgcttt aaacaaatct aacattggtg gttgttatcc tgtgtatctg ggttatcagc
    11821 gaaaagtata aggggtaaac aaggataaag tgtcactctt tagctaacct tgcatcgcat
    11881 tgaccaaaac ttgaaccgat ttagcaaaac gtggcatcgg tcaattcatt catttgactt
    11941 atacttgcct gggcaatatt atcctctgca actaattact tgccagggca actaatgtga
    12001 aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc catatggtta
    12061 atcagaagaa agatcgtctg cttaacgagt atctgtctcc gctggatatt accgcggcac
    12121 agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt gaactgaaaa
    12181 aagtgttgtc ggtcgacctg ggagcactga cccgtatgct ggatcgcctg gtctgtaaag
    12241 gctgggttga aaggttgccg aacccgaatg ataagcgcgg cgtactggta aaacttacca
    12301 ccagcggcgc ggcaatatgc gaacaatgcc atcaattagt tggccaggac ctgcatcaag
    12361 aattaacaaa aaacctgacg gcggacgaag ttgcaacact tgagcatttg cttaagaaag
    12421 tcctgccgta aacaaaaaag aggtatgacg atgtccagac gcaatactga cgctattacc
    12481 attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc actggagaaa
    12541 gtgtcagagc gttcgggtta ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc
    12601 ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc gcaaaagctg
    12661 aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga gtcgcaacaa
    12721 actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata ccggatgacc
    12781 aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaatag ctagttgata
    12841 acgtgacaac gtcactgagg caatcatgaa accactttca tccgcaatag cagctgcgct
    12901 tattctcttt tccgcgcagg gcgttgcgga acaaaccacg cagccagttg ttacttcttg
    12961 tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt taaatcacat
    13021 gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc aacacgctat
    13081 gtagttattg tcaggctctg atcacagagc ctgacatctc cgtaatatct tcctcaagtc
    13141 tcaaatattt tcgccattgt tggcgatgag atcccgatac cagtaaaacg acgctttacg
    13201 tatccgttcc agcgttccct tcccttcgtt gtctttatcg acataaatca ttccgtagcg
    13261 ctttttcatt tcccctgttc cggcagaaac caggtcaatg cagccccacg gggtgtagcc
    13321 aattaagtca acaccatctt caactacggc ttttttcatt tcccgaatat gggaagcaaa
    13381 gtaatcaatg cgatagtgat cgttcaccgt gccgtcagct tgtctctggt caaccgcacc
    13441 aaatccattt tcgacaataa acagcggcaa ctggaaatga tcccagaacc agttgagtga
    13501 ataacgtagc cctgccggat caatttgcca tccccattcg gatgtatcga tataagggtt
    13561 gctgaccaaa tctcgtggtt caacataatc cagttgcggg ttatcgggag aaaattgtgt
    13621 cgtaaaagac atgtagtagc taaaaccgat aaagtcgaca catccactgg caagaatcgc
    13681 gttatcttct ggtgtgatat cgaggttgaa tcctttcctg gcaaagtaat tcagcatatg
    13741 ttgcggataa tatccacgag catgaacatc agtaaaccag taacgacgat gcatcgcttt
    13801 cgtggccatc atcatatcgt tgggtgcaca cgtcagagga tagatggggc acatggcgat
    13861 catacagcca atgttaaatt caggattgat cgattttcca gtctgtaccg ccagggcact
    13921 ggcaactaac tcgtaatgta ccgcctggta cattatttgt tcgcgctcat tgatatcttc
    13981 ctctggcgaa tacaagatac cggaattagt aaatggacac aggctttcgc tgaaattcac
    14041 ctgattattg atttcgttaa acgtcatcca gtactttact ttttctttat agcgcgtgaa
    14101 gaccgttgat gcgaagcgga taaaaaagtc gatcagctta cggtttcgcc agccaccata
    14161 ttttgtcacc agatgataag gcatctcaaa atgcgaaagc gtcaccacag gttccattcc
    14221 ttgcttcagg cattcatcga acagatcatc ataaaattgt aaaccctctt cattcggctc
    14281 ctgttcgtca ccttgcggaa agattcgtgt ccaggcaatg gaagttcgaa agcatttgaa
    14341 tcccatctcg gcaaataact gaatatctgt tttatagcga tgataaaaat caattgcttc
    14401 atgattggga taattaagcc cgtcgataac gccttctgtc acttcacgcg gcaccccgtg
    14461 agcgccagca gtcatcacat cagcgatact gatgcctttt cctccttcat tccagccacc
    14521 ttccaactga tgcgcggcta ccgcgccacc ccataaaaaa ccttttttaa atcctgacat
    14581 acacaactcc ttaactaaaa tagattacca ccagacttc
//
LOCUS       DAQSWQ010000107        14390 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.108, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000107 DAQSWQ010000000
VERSION     DAQSWQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.108"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            255..2021
                     /gene="adeD"
                     /locus_tag="R8G18_003339"
     CDS             255..2021
                     /gene="adeD"
                     /locus_tag="R8G18_003339"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEE9796953.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="R8G18_003340"
     CDS             complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="R8G18_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HEE9796954.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="R8G18_003341"
     CDS             complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="R8G18_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HEE9796955.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="R8G18_003342"
     CDS             complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="R8G18_003342"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HEE9796956.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="R8G18_003343"
     CDS             complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="R8G18_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HEE9796957.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="R8G18_003344"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="R8G18_003344"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEE9796958.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="R8G18_003345"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="R8G18_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEE9796959.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="R8G18_003346"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="R8G18_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HEE9796960.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="R8G18_003347"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="R8G18_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HEE9796961.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="R8G18_003348"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="R8G18_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HEE9796962.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="R8G18_003349"
     CDS             complement(11403..11900)
                     /locus_tag="R8G18_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEE9796963.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="R8G18_003350"
     CDS             complement(11897..12259)
                     /locus_tag="R8G18_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEE9796964.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="R8G18_003351"
     CDS             complement(12249..12596)
                     /locus_tag="R8G18_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HEE9796965.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /locus_tag="R8G18_003352"
     CDS             12705..13154
                     /locus_tag="R8G18_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796966.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13403..>14390)
                     /locus_tag="R8G18_003353"
     CDS             complement(13403..>14390)
                     /locus_tag="R8G18_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEE9796967.1"
                     /translation="PSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGV
                     KLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPS
                     ASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRK
                     IQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSLPGRNVRAK
                     SGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCVCCGHTAKENRLSQSK
                     FRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3395 a         3970 c         3731 g         3294 t
ORIGIN      
        1 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
       61 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
      121 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
      181 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
      241 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
      301 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
      361 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
      421 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
      481 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      541 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      601 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      661 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
      721 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      781 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
      841 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
      901 cgggtttggg tggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
      961 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
     1021 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
     1081 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
     1141 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
     1201 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
     1261 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1321 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1381 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
     1441 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1501 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
     1561 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
     1621 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
     1681 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1741 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
     1801 tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1861 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1921 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1981 tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
     2041 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     2101 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
     2161 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     2221 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2281 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2341 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2401 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2461 cgatgcaggc caccaggcca cggcgaccgt tcgctaggtc agagagccag ccccacagca
     2521 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2581 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2641 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2701 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2761 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2821 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2881 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct gctgcgcctg
     2941 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     3001 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     3061 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     3121 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
     3181 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     3241 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
     3301 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3361 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3421 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3481 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3541 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3601 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3661 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3721 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3781 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3841 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3901 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3961 tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac cgataaatcc
     4021 acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     4081 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     4141 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     4201 acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt gtcgccattc
     4261 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat
     4321 tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgtag
     4381 tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4441 taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4501 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg caaacgccca
     4561 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4621 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4681 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4741 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4801 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4861 atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4921 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4981 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     5041 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     5101 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     5161 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     5221 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     5281 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5341 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gttgcccgct
     5401 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5461 agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5521 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5581 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5641 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5701 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5761 cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5821 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5881 gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5941 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     6001 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     6061 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     6121 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     6181 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6241 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6301 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6361 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6421 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6481 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6541 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6601 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6661 ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6721 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6781 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6841 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6901 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6961 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     7021 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ctccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattac tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cggcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctggggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtgga cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc aggctgaccg tacccgctgc tgactttgac atgttgctga
    13801 ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt
    13861 gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct
    13921 gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca
    13981 gcttcttctg gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg
    14041 tggcgagttt agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag
    14101 ttgatacttc actttctgtc tgaatactga tgtaccactt accgcaggac tggctgacag
    14161 tgacattttt cacaacaccc gtgacctgac ggctgttccg gtagcgcatc cagcccagtt
    14221 tcggcagaaa aatacggctg ttttcctgat cgagcttaac gccctgcggg tagcggaata
    14281 catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgattctgg aagaagtttt
    14341 tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtgacggg
//
LOCUS       DAQSWQ010000108        14368 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.109, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000108 DAQSWQ010000000
VERSION     DAQSWQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.109"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..696)
                     /locus_tag="R8G18_003354"
     CDS             complement(<1..696)
                     /locus_tag="R8G18_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEE9796968.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="R8G18_003355"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="R8G18_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HEE9796969.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="R8G18_003356"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="R8G18_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEE9796970.1"
                     /translation="MSEQLTDLVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="R8G18_003357"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="R8G18_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HEE9796971.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="R8G18_003358"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="R8G18_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HEE9796972.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="R8G18_003359"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="R8G18_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HEE9796973.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="R8G18_003360"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="R8G18_003360"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEE9796974.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="R8G18_003361"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="R8G18_003361"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEE9796975.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="R8G18_003362"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="R8G18_003362"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEE9796976.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="R8G18_003363"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="R8G18_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEE9796977.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="R8G18_003364"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="R8G18_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEE9796978.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="R8G18_003365"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="R8G18_003365"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEE9796979.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="R8G18_003366"
     CDS             10119..10499
                     /locus_tag="R8G18_003366"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEE9796980.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="R8G18_003367"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="R8G18_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HEE9796981.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="R8G18_003368"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="R8G18_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HEE9796982.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="R8G18_003369"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="R8G18_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="R8G18_003370"
     CDS             complement(12845..14032)
                     /locus_tag="R8G18_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEE9796983.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14368)
                     /locus_tag="R8G18_003371"
     CDS             complement(14032..>14368)
                     /locus_tag="R8G18_003371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796984.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3620 a         3608 c         3707 g         3433 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggacctggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagcct ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 tctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggttta
//
LOCUS       DAQSWQ010000109        14297 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.110, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000109 DAQSWQ010000000
VERSION     DAQSWQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.110"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="R8G18_003372"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="R8G18_003372"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HEE9796985.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="R8G18_003373"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="R8G18_003373"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEE9796986.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="R8G18_003374"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="R8G18_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HEE9796987.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="R8G18_003375"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="R8G18_003375"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HEE9796988.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="R8G18_003376"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="R8G18_003376"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEE9796989.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="R8G18_003377"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="R8G18_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HEE9796990.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="R8G18_003378"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="R8G18_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HEE9796991.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="R8G18_003379"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="R8G18_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HEE9796992.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="R8G18_003380"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="R8G18_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEE9796993.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="R8G18_003381"
     CDS             complement(13978..>14297)
                     /locus_tag="R8G18_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEE9796994.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAQSWQ010000110        14159 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.111, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000110 DAQSWQ010000000
VERSION     DAQSWQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.111"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..228)
                     /locus_tag="R8G18_003382"
     CDS             complement(<1..228)
                     /locus_tag="R8G18_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HEE9796995.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="R8G18_003383"
     CDS             385..561
                     /locus_tag="R8G18_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HEE9796996.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="R8G18_003384"
     CDS             1187..1486
                     /locus_tag="R8G18_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9796997.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="R8G18_003385"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="R8G18_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEE9796998.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="R8G18_003386"
     CDS             complement(2472..2939)
                     /locus_tag="R8G18_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEE9796999.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="R8G18_003387"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="R8G18_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEE9797000.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="R8G18_003388"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="R8G18_003388"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEE9797001.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="R8G18_003389"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="R8G18_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HEE9797002.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="R8G18_003390"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="R8G18_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HEE9797003.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="R8G18_003391"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="R8G18_003391"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HEE9797004.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="R8G18_003392"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="R8G18_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HEE9797005.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="R8G18_003393"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="R8G18_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HEE9797006.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="R8G18_003394"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="R8G18_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HEE9797007.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="R8G18_003395"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="R8G18_003395"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEE9797008.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13837..14082)
                     /locus_tag="R8G18_003396"
     CDS             complement(13837..14082)
                     /locus_tag="R8G18_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEE9797009.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3446 a         3427 c         3689 g         3597 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgac
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cctggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgta
//
LOCUS       DAQSWQ010000111        14075 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.112, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000111 DAQSWQ010000000
VERSION     DAQSWQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.112"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(406..1851)
                     /locus_tag="R8G18_003397"
                     /pseudo
     CDS             complement(406..1851)
                     /locus_tag="R8G18_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(2003..2602)
                     /locus_tag="R8G18_003398"
     CDS             complement(2003..2602)
                     /locus_tag="R8G18_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HEE9797010.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(2670..4199)
                     /locus_tag="R8G18_003399"
     CDS             complement(2670..4199)
                     /locus_tag="R8G18_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HEE9797011.1"
                     /translation="MRFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRA
                     INSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKR
                     IADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKSAFVEAVGRASDD
                     AEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLKD
                     QSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENTPAGMQSQVLLA
                     ADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLT
                     AKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTVGKAFPRDSRAPER
                     WPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHERENNDIYSSCRLIVRKNGAEIYNRT
                     ALDNTLIYSGVIDMPAGRGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGISI
                     S"
     gene            complement(4205..5848)
                     /locus_tag="R8G18_003400"
     CDS             complement(4205..5848)
                     /locus_tag="R8G18_003400"
                     /inference="COORDINATES: protein motif:HMM:NF024939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HEE9797012.1"
                     /translation="MEVTKAKPVTRTITSANIDRLRVTFGVQSLVQTTSKGDRNPTSV
                     RLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVRETADSTTDQL
                     QNKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDP
                     EKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCD
                     QTVPDSFGETEPRMTFNAYLAQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDV
                     VWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILR
                     YGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICD
                     NDYAGTLTGGRVLSIDAATRTLTLDREVTLPETGAATVNLINGSGKPVSVDITEHPAP
                     DRIQVSTLPDGVETYGVWGLSLPSLRRLLFRCVSVRENTDGTFAITAVQHVLEKEAIV
                     DNGARFEPQSGSLNSVIPPAVQHLTASIWRRRNGTRRGW"
     gene            complement(5918..6121)
                     /locus_tag="R8G18_003401"
     CDS             complement(5918..6121)
                     /locus_tag="R8G18_003401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797013.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGLIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAV"
     gene            complement(6465..7145)
                     /locus_tag="R8G18_003402"
     CDS             complement(6465..7145)
                     /locus_tag="R8G18_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HEE9797014.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(7043..7786)
                     /locus_tag="R8G18_003403"
     CDS             complement(7043..7786)
                     /locus_tag="R8G18_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9797015.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(7797..8495)
                     /locus_tag="R8G18_003404"
     CDS             complement(7797..8495)
                     /locus_tag="R8G18_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HEE9797016.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(8495..8824)
                     /locus_tag="R8G18_003405"
     CDS             complement(8495..8824)
                     /locus_tag="R8G18_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9797017.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(8821..11394)
                     /locus_tag="R8G18_003406"
     CDS             complement(8821..11394)
                     /locus_tag="R8G18_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEE9797018.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(11375..11788)
                     /locus_tag="R8G18_003407"
     CDS             complement(11375..11788)
                     /locus_tag="R8G18_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HEE9797019.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(11815..12246)
                     /locus_tag="R8G18_003408"
     CDS             complement(11815..12246)
                     /locus_tag="R8G18_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HEE9797020.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(12260..13012)
                     /locus_tag="R8G18_003409"
     CDS             complement(12260..13012)
                     /locus_tag="R8G18_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9797021.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(13020..13415)
                     /locus_tag="R8G18_003410"
     CDS             complement(13020..13415)
                     /locus_tag="R8G18_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9797022.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(13412..13987)
                     /locus_tag="R8G18_003411"
     CDS             complement(13412..13987)
                     /locus_tag="R8G18_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9797023.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(14002..>14075)
                     /locus_tag="R8G18_003412"
     CDS             complement(14002..>14075)
                     /locus_tag="R8G18_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9797024.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         2822 a         4401 c         3505 g         3347 t
ORIGIN      
        1 ttcgataacg gcagcaacgg tcgatttcgc aagaatatcc cgcccgactt ttgtcagggt
       61 tgccaggctg gcgacatcat tccccgtaaa atacggaaac ttgcctgccg cagtagtaag
      121 tccggccagc gccgtcaggg tggcatcttt cggttgctta cccgcaagcg cgttagtcat
      181 ggtggtcgca aaattcgggt cgttgcccag cgccgcagcc agctcgttca gcgtgttcag
      241 tgcatcaggt gacgaatcta caagggcggc aatcgcagcc ataacgaaag ccgtgcttgc
      301 gatttgggta ttattcgttc cctgtcgcgc agttggtgtt gttggcgttc cggtcagtgc
      361 aggactattt aagggcgctt tcttgttcgt ttcacccatt accgccttaa ccgcttttgg
      421 cgttgccgcc agtgactcgg aagtgctgtt ggtcgcactg ctgagctgta ctatcccctt
      481 tttcgtcgtg ctcgcatcct ccagcgccac ggcggatgca atatcctctg cccgttttgc
      541 tgctgtctcg gcgcgcgttg ccgcggattc agcagcaact ttgctctgag atgcagccgt
      601 cgcactgcct gccgcctctg atgctttcgt tgttgctgtc gtggcactac ctttcgctgc
      661 tgacgcttgt ctggtcgcct catcttttga agcagacgca gatgatgccg atgacgccgc
      721 tgaactggct gacgatgcgg ctgctgcctt agaggaagca gcattgtctg ctgaagtctt
      781 tgcatttgtt tcagaggttt ttgctgcaga agcagacctc gctgctgcag tggcttgctc
      841 agtggctttg ccagccttcg ttgtggctgt tgaagcggat gatgcggcgc tttctgccga
      901 ttttccggcg gcggtggcac tggctgaggc ctgcccggca cttgttgacg cggcactggc
      961 agataatgca gccgctgttt ttgaacctgc cgcagctgag gcgctctgtc ccgctgctgt
     1021 ttcagaagac ttagcgttcg tctcggacgt ttttgccgcc ttcgcggaat ttcctgccgc
     1081 cgttgccgag gaagctgcac tactggcact tgatgatgca ttcgtttctg aagatttcgc
     1141 tgcctctttt gaggccgccg caccccgtgc cgaggtggca gcttctgacg ccttcgtggt
     1201 cgctgtggat gcagaagtgg ctgccgattt ttgtgatgct gcggcattcg tttctgacgt
     1261 tttcgcggca ctggcgctgg tagctgccgc gctttttgat gactctgcag cagcactttt
     1321 tgctgcttca cgggcctttg tcgatgccgt tcctgcgctg gaagacgctg actgagccga
     1381 cgacgcggcc tgtccggctg acgtgctggc ggcacgtgct gaggctgcag catcggttgc
     1441 atgagttgcc gcctcgctgg ctgatttttt cgcggctgcc gtattctgtg caaccgcgga
     1501 ggcgttacgt gacacctctt ccaccatctg ctcaaagcgg cgcagtgcct ccggtcggac
     1561 atcatcctcc gtcatggcac cgagaaaatc attcagcgta cctggtctgg aaccttcata
     1621 gacggtaatg gtcccggcat gtgaaggcgg aaaaccttca accagcaggg tgacgctgta
     1681 ctgaccatgc tcaacatcca tgctgtaacg tccggcttca tccggatttt cagaggccac
     1741 cgtgttcacc accatcgtgc tgctggttcg tctggccttc agcacaatgg tgcagttctg
     1801 tactggtttt cctgtgccat ctttaagcac gccagaaatt tttactgtca tacttttcca
     1861 ccaataaaaa aagcccgcag cagtgacgcc acgggcttca ggacagtgta actttacgtt
     1921 tcctcaaacg cagttcaccc cataaggtgg atgaacctgc gtatcataac aatatttaca
     1981 gaagataaat cggcgtctgt tgtcagatac ggtatccgat accaacaata aatgcatccg
     2041 ttcgccagtc accactaccg gaaccttcat aagcaaggtc aatggtcacg gattcggtcg
     2101 ggttaaactg cacgccagcc ccccacgcca gagacgtgtt gctgtggcga ccgtcatcac
     2161 ttccggtcag cacatcgtgc gttttcccct tgttgtcagt tacgcggaga taatccccgg
     2221 agaaagtcga cacacggctg taagccacac ccaccatcgc atacgcgctg aaccattcat
     2281 tcacgcgtac agacggcccc gccatcacgc tgaaccagcg gttacgcacg gaatcttcat
     2341 gccagcgggt atcgctgtag tgcgtttttt gctcatcctc agcattggca taactgaagg
     2401 acgtaatcag ccccagcgcg tccgtaaact cataacggta tttcacgtta atcccgttca
     2461 gattatcgct gccgggagcg ttcgtacggg catgaagata ccccgcgctc agtgtggact
     2521 gatgttcaga cgcccatgca ggcgcaccgg atacggacag acagatggct gcggacaaaa
     2581 tggctgcaca aactttacgc ataattacct ctcgcttttc tgcaataaaa aaggcgccat
     2641 ttctggcgcc cgtatatggg ttataaaatt cagctgatac tgatgcctgc ggtggctttc
     2701 ttcatcacca caaccagcaa atcgctgata cttgctgtgg gataccagtt attcaccagc
     2761 catgctgaca ccgaaaactc cagcgtcatg tggccgcgac cagctggcat atcaataaca
     2821 ccactgtaaa tcagcgtatt atccagcgcg gtacggttat aaatttcagc accgtttttc
     2881 cgtactatca ggcggcatga cgaataaata tcgttattct cccgctcatg tttagcgccg
     2941 ctgaatgcca ccgccggaat aacaatttgc ctgtcaaaag gctgatcgtc ataaatcctg
     3001 acggtaatgg tccctgatgg ccaccgctcc ggtgcccggg agtcccgcgg aaaagcctta
     3061 cccactgttt tgactatatc gccttcaatc tggttggctg acagtttccc cttaatctga
     3121 cagttctcat taattgtgac attgttgagc gtcccggcgt tcgcattcac actgccactg
     3181 atatctgcat ttttagcggt cagttttccg tccggtgtca gggaaaatgc cggaggatta
     3241 ccgccgctgg taatggtggg agccgtcagg cgtttcagga acacgtcgtt catgaatatc
     3301 tgatcgccct gaccaacaaa catcggcttt gtgttgccat tcgcaggatt aatcatcgca
     3361 atcctgtctg ccgccagcag cacctgactc tgcattcctg ctggcgtatt ctcaataccg
     3421 gcaccgatac ccgcaatata aaggcgtccg tccttcatct gttgcagctt cacagcccac
     3481 atgctgttca gattattatt tgtatcaacc tgaaccttct gtatctgctg gattgccgca
     3541 ctctggtctt tcagtttctt attgacagtc tgcgtgattt cattgctgac atccgtaatg
     3601 gacgtcctga tttcagccag gtcaggcgca agctgaccgt tatcaatctg cgtccacagc
     3661 tcctgagcca gatgtgtttt ccctatctct cctttgaaaa aatccaagta accttccgca
     3721 tcatcgctcg cccgaccgac agcctccacg aatgccgatt tgccaacggt gttcacactg
     3781 cgaacgtaaa aataataatc atggcccggt ttgatattga tactggcggc tatccagtac
     3841 agcgccgtgc caagatagcg ggcgctggtt tcaacctgcc tgatatccgc aatccgcttt
     3901 tccgagaacc agaactcaaa ctgtaccgtc gggtcataaa cggcaagatg cggcgttgcg
     3961 gttatctgaa aatagcccgg cgtcagctca atccgcgacg gtgctgccgg tgcggcaatc
     4021 cggaaggtgg tggtggcagg ttcaccctgc tggccatagc tgtttatcgc ccgcaccgtc
     4081 agggtgtatt ccccgagcgg caggccgctg aaacggtgct ccgtgtctgc ggtgatggcg
     4141 gtggtcacca gtctggcatc cgttccctta ccactggtca ggcgcagact gaagcgcaca
     4201 cccttcacca cccgcggcgt gtcccatttc gcctgcgcca gatactggcc gtcaggtgct
     4261 gcactgccgg tgggatgacg ctgttcaggg aacctgactg cggctcaaag cgggcaccgt
     4321 tatccacgat ggcttctttt tccagtacgt gctgcaccgc cgtgatggca aaggtgccgt
     4381 ccgtgttttc ccggacggag acacagcgga acaggagacg gcgcagtgac ggcagggaga
     4441 gtccccacac cccgtatgtc tccacaccat caggcagggt actgacctgt atccggtccg
     4501 gcgcggggtg ttcggtgatg tccacactca ccggcttacc gctgccgtta atcaggttca
     4561 ccgtggcggc accggtctcc ggaagtgtca cttcacggtc cagcgtcaga gtgcgggtgg
     4621 cagcatcaat ggacaggaca cgtccgccgg tcagggtccc ggcatagtcg ttatcacaga
     4681 tttcaatgat gtcaccgggt gtgtgccgca gcccctgaga cccgagcgtg aaatccaccg
     4741 tctgcgtttc cagcagttcg gtctttatca cccacagtcc ggcacggtgg gcctgaccgc
     4801 ggctggtaca gccgaacgcg tccatcttca gcaggttgcg tccgtagcgc agtatggctt
     4861 ccgggtcttc caccagttcc gtggaggtct gccagccgtt ctgcgggtcg gtataattca
     4921 cctccaccgc cgtgtgccgg tccttcaggg cactgaagct gtagcggaaa cccacgccgt
     4981 tatcatccac caccacatcg ctgttggtgt acggccacac cacatccgac gggcggtcct
     5041 gaacaaacgt cagcgtctgg ccgttccata ccggcataca gcgcatcgcc gagcagaaat
     5101 cactgagcac gtcccatgcc ttacgctgtt gtgccaggta cgcattgaag gtcatccgcg
     5161 gctcggtctc cccgaaacta tccgggaccg tctggtcgca gtactgcccg atggcataca
     5221 gcgcccactt gtccacatcc gccgccccca gacgttttcc catgccgtag cgcgggtgag
     5281 tcagcatgtc ccacagacac caggccggat tgttgctgta tgccggtttc aggctaccgt
     5341 cccagatacc actgtacgtg cgtttttccg ggtcatagtt tgacggcacc tggatgatgc
     5401 gaccgcggat atggtagttc accgtcatct gctggccgcc gaactgctcc gcatccacct
     5461 gcagccccac aatggccgtg ttcgggtagc actgtttcac atcgatgatt tcggtgtatg
     5521 acgaccacag cgtcttattc tgcagctggt ccgtggtgct gtccgctgtc tcccggacca
     5581 tccggatgtt aaaaggacgg ggaggcagat tatccagaat caccgacgcc agaaactgcg
     5641 aggtggtctt gccgttaatg gtgacatcct tttctgtcac ccagttaccg ttacgctgta
     5701 actgaatcag caggcggacg gatgtcgggt ttcggtcacc ctttgacgtg gtctgcacca
     5761 gtgactgcac cccgaaggta acccgcaggc ggtcaatgtt cgcggacgta atggtgcgcg
     5821 tcaccggttt tgccttcgtc acttccacgc ccagtccggt ttcagcaccg gaggactcaa
     5881 agccttccgg tggtgtctgc tcctgctccc cggcgcgtca gaccgccgtc acaccgtgta
     5941 tcacgggatt accgtccgtg tccgtcagcg gggttttgtt caccaggata ctctgcagtc
     6001 ccttcaccgg accttctatc agtccctcac caatcgcatc aatcacgctc atcatctgcg
     6061 tggatttgag attatccttc gcctcacgag gcgtgtgtgc cttaccgcca ccttttccca
     6121 tacagccttc ccctgaataa attaaccgcc acttgccatt ccgtacagaa gtcggatatc
     6181 cttcgcccga aaagcatgaa acacatttct gccatgctaa agagaaaccc cggtatcagc
     6241 agataccggg gttttctttc atgcccaccg ataatcctgt tggttaaaac cggtaatggc
     6301 ataaaaattc tgaatatctt cacattttca caaactgact gtggcgcgta taatttctct
     6361 gcgttaattt ttttgtcgtg atataagaat aattccttac acttaatctt cgtaactctc
     6421 ccgcagttcc tgtccgcgat cactgcggga tttttttatt ctttttaccc ctgccgcccg
     6481 ataaccacga cctttccgcc cccgccttca tcacgggtgc tgatgtcctg ggatatacgg
     6541 cgggagccaa ccagcatttc cccgtaaggc accggcatcg ggttcccctg ggcaatcatg
     6601 ttatccagtg aggaaaagta cgtgttctgt ctgccgttat ccgttgcgcg gtaatccggt
     6661 gtttttgcct tcggggccag catctgggcc acaccgccca gaatcatgct ggctccaagt
     6721 gaaaacagca tcgtggtggc agaaaaacca ccggctgcca gggctgaacc ccataacgcc
     6781 attgatgccc cggcagtgaa gaaagagccc acgatggctg ccgcccccag cacaatctgc
     6841 agtccaccct ttccggcccc ggccagtcgc ggcacaatgt ggatgaccgt tccctcaccc
     6901 agctgttcgt gaagacgggc gtacaccgcc tccggtgccg tgtcataacc ggcaatacgt
     6961 atctggtacc agccttcgtt catctgacgg cgaaagcccg gcatctgcat cgacagggcg
     7021 cgaatggctt ccgctgccgt gttcacatac aggctgaggc ggcggccaaa tcgttgtaaa
     7081 tccccgtgaa ggcagatgcg tgccagtggc ggtgacgcca gactgaatgc gttcgtcgtt
     7141 gccatttttc ggaatacctc tcccgtttac tcagttgttc aggcagatgg tgaagcagct
     7201 caccgttgcc gcagtaaatg gcggcatggt tcggtaccga agcaccaaag cagcacagca
     7261 gaatatcgcc cgcctgtgca gaggacaggg gcacccggta aaagccggtg accgccatat
     7321 tgtccaggta aaggttctga ccgttgcgcc accagtcatc ctcgcgatga aaatccggca
     7381 tttcagtccc cgccagatga taagcatccc ggaacagcgt gtaacagtcc gtcaccccgt
     7441 gctcaaagcg ccgtcctgtc agatgtggca cacagcggaa tttgtgaatg tcaccccggc
     7501 agaccaacca ccagggcagt gcgcttttta tctgcaaccg ccggtcagcc tcgctcagcc
     7561 agggcagccc accgggatga ctgtggacca gtgccacaat ctccccctgc atctctgccc
     7621 gcagccagtc ttccggtgcg atacgaaaat acgcctccgg ctctgcggaa atattcacac
     7681 aagggatata ccgctccccc tccggcgtgc ttatcacgaa gccgcacgac tccgcaggcg
     7741 cacaccgccg ggcatgtgcc agaatcgctg attcagtctg tgtcataaac cgggatttac
     7801 tgcgaaagtt tattaatgga aaggaaaccg ccaaaattgc cgacattcct gcgcagttca
     7861 cacccgcgca tgcacttgct gcatctgtcc ttacggatat ccgtggtggg gttgtcgaac
     7921 tcatccgcca ccgcaggacc gttatacccg cattcatctc cccggtaatc ccacatacag
     7981 gtgttcgcca gcatgatgcg accgggaaac agcgccccgt ccgtctcggt cggtgtagcc
     8041 agcacaaacg aggccgtcat ggctgtcagc tgcgacatct gctccaccac ccagcggtca
     8101 ctcagctcct gctccgggtc cgcctccgga ttgcccgcaa cgaaattcac cgcatccaga
     8161 aaacgggcat acacccggcg gcggaccacc gtggccccca ccagactctg caggtcttcc
     8221 gccatcccgg tgaccagacc gaacagattg gacaccgtca gcgacggtct ggcactgctg
     8281 ccccggccgt tcatctcaaa gccgctgccg tcaatcgggt atgcctcata cttacgcccc
     8341 tgccaggtga ccggctcccc tttttcattc agctcattac agaaaaaata ccgctcacca
     8401 ccctgtaccg tcaggtcgat ttcccagagt accacccgcg gtgactgctc tgacttaacc
     8461 gactcgttca gactttcttc gcgaatatcc tgcatcagtt caccacctgc ttaaactccg
     8521 cgctgaactc aacgcgcaac atcccgaccc gcgcagacca cccggcacag gtcaccttta
     8581 tctgccggta tgcatagggt ggcttccaca aaaatgcctt ccagccaccg tgctctgcca
     8641 ggaacgcttc cagatgccgg gcctcctccc gggtcacgga aagcgtcacc ctgtatgttt
     8701 tcaggtcagc attcagccct gccgccatac gctgtgagta cccgtcacca aaacgcactt
     8761 cacgcaccga tggctgcgag ttcacctcca tatccggctt cactttccag cgaaaggttt
     8821 tcatccaccg ctccctgata acataccgcc atcacgcaac tgcagccgga gttcatcctg
     8881 tgcccccttg cgggccatct catacaccgc tttcatcagc tgcggccctg cccgcccgtt
     8941 ggggccgtcg ttctgaatca ccacgtgatt gttctgatta aaattaatgc cttccgcccg
     9001 ccgcatctgc gccggacttc cggcaccgcc gacataacca ccttccgcat agccccgcat
     9061 caggcggtac agattgccga caccaatccg gctggtcgcc tccttcgtga agacaaactc
     9121 cccgcgatga acaatccccg caggttcata tttacccccc gtccccgtaa atcccccggt
     9181 cgcgaaatgg aagttcgccg ccgcagcctg aatggccgtc cccgtggagg cagacgcacc
     9241 accaccgaaa gcaccgccaa tggcgctgcc gatactcccg actatcccca ccatcgcctg
     9301 cttcagaaaa atctctgtca gcatggacag cacagaacgg gtgaaaccac gccagttctg
     9361 ttcgctgccg atcagcatcg ctgccatatt ctgtgcaata ccgtcaaagg tctgcgtggc
     9421 cgcgttttta acctgcgaaa aactgtccgt cgcactttcc gcccactcgc cccagccgga
     9481 cttcatcccg gccatccagc ttccacgaag ctgctcctcc gcagaccagg tgttcttcag
     9541 tgcagatgtg gccttcgcca gcgcagccgg attatcaccg tacacctcac gaaggcgctg
     9601 ctcttccgac tcccgctgcg cctgacggtc ggtgagtccg cgggcttttg cgctgattgc
     9661 cgcctgcttc gcgctctgct gctgttcaaa ccgcgccgcc tgctgtgcca gctcattcag
     9721 ccgtttctgg tgttcaatct tgtcgcccag ctcagccagc tggcgtttgt actccagcgt
     9781 ttctttctca tgggccagca gggatttttc ctgctcagat aactgccgtt tcgtggcggc
     9841 ctctttcagg accacatact gattttccgc tttccataaa tcacggcgct gctggctgat
     9901 tttttcattc gcaccgctgt gcttctccag cgtcctgagc tcagtttcaa gcgccagcag
     9961 ggctgcatgc gcctggtctt cctggcgctc accggcagac accttcacac cggacggctt
    10021 tttctgcgtc gactcataat ccttttttgc cgacgccatc agcgtgttgt aatccgcctg
    10081 caggattttc ccgtctttca gggccttatt cagctcttcc tgccgggcgg tatatttctc
    10141 cagtggcgtc agcaggcgct catacgcctt ctgcgcctct ccggtatact tcagctgtga
    10201 cgactcacgc tcagccctgt cccttgccgc cagttcaccg gctttttcca tatccgactg
    10261 cagcgttgcc gctgccagac ccagacgggc attttcccgg tcatcccatg cgccctgaag
    10321 gttggcccgg aaagaggagg ttttaccgcg gcgctggctc cgactctggt accactgcca
    10381 ttttttatcc gcctcatcaa atgccttctg cgcactggcg agcatatccg ctgaggattc
    10441 cggacgaccg atatccagaa tggcatccca catcgatttg aatgccttcc ctgttttatc
    10501 cgcccaggtc tccagtgttc ccatgttttc tttcaggcga cgggtctgct catcaaagcc
    10561 tttcgtggcg atatcgttcg ccgcctgcaa tgccccggcc tcgtctccgg aacgctgcag
    10621 ctgtgcaaca tacgcaatct gctctgccgt cacgttacgg aactggcgcg ccatcgccat
    10681 cagtcccgac gtcgggtcag tggtcagctt cccgaaggct tcagcgactt tatccacctc
    10741 cacaccggat gcagacgcaa aacgcgcgac actctggttg atggcatcaa actgttcacc
    10801 accacgcaca ccggcattca ccagggctgc cagtgactct ctcgcctggt taaacgtcag
    10861 ccctgctgcc tgcccggctc ttgagagagt cagcatacga tcggcagtca gtccggactg
    10921 attaccggaa agaaccaggg ttttattaaa cgctgaaagc gtggaatctc cctggtacca
    10981 ggcgtacacc agtgcacctg tcgccaccgc cagcgaggtg accccgacca tcggcagggt
    11041 gatcgcatcg gcaagccccc tgaacatggg gatcatcccg ccgaaggagt ccttcacctg
    11101 accgccctgt tgcagcagga tcagccaggg attctgacca ccggcaagct gcgtggcgat
    11161 atccgtaaac tgtgcgggca gggttcgcat ggccgcttta tactgcccga cggaaatccc
    11221 ggctttttgt gcagccagcg cctggcggct caggccctgt tcaacagcac tggcggtttt
    11281 tctggcgtcg gtatccagac ctgaaaaatg acgccttacc cggctcatct gctcatcgaa
    11341 acggacagca tccagactca ggtcaataac aagatcacca accggctggg acatatctca
    11401 cacctcccgg aatccccgct gaagccatca ttaatgcggc atcatccacc atgacatccg
    11461 ccacatccgc agacgataaa atatcgcgcc ctccgtcccc accgaaccgg acgcctccgg
    11521 caagtcctgc cgctttctgc atcagcattt tgtcctcatc cggcctctcc acctgctctt
    11581 cctcatgccg ggggacaagc agactgaaat cagagggatg catatccgga tcgcaaaaaa
    11641 acaggctgag tacagcgtac gtcagcccgg aaaaatgcat atccagctgg gtatcctgaa
    11701 aataatgcgt gcggtaaaaa cggcgccagt cggcatattc ggtggatgtc atcccggcaa
    11761 gcatggcgcg ccagtcgggt ctccccatct cacgcgccag tctgagggca aagttcagct
    11821 cgccgtcgaa gactttcccg cagaaaaatc atcatcagtc agcgtgttat ttttcgccac
    11881 ttcagtaata tcagtatccg gacgaacagc ttcgatcatc ccggacaggc acaacaccac
    11941 gtcttccgcc cgggcaatgg catcggcagg ccaggtggtg agcacttcct gctctatctt
    12001 catcacggcc tcattcattg acggtgactg cgttttctgt ggatggttat gccacaggga
    12061 catcgccacc agaaacgcgc cggttctgac gagatcttcc acgcttacct gcaggttgcc
    12121 gctggattct gcctgttctg cacgccgttt caggagggca agatgctcga tacgctgcag
    12181 cgcagacaat tcggaaagcg tgacagacac accgttatat tcaaattgtt ctgttttcag
    12241 aaacatgtat tacctccgtt taccctgcag cgcccgcttc agtaacggtg acttcagcca
    12301 ctgcggcgaa ctgaccattt ccgctcacca cagggatctg caccttacct gtcgccacgc
    12361 cgtttaccgt aattgtcata tctttcacac taatggtggc tttcgacgga tcggcggaaa
    12421 ccgctctgaa cgtcttgtcg gttgcacttt ccggctcaaa agaaaccgtc agggtggttg
    12481 ttttcccttt tgccaccgta ccggatgtcg gcgtcacctt aatcgcactg accggcgtaa
    12541 ttttgctgcg ttcttccgct acagaaggtt tacccacgtt agtgactttc accgtgcggg
    12601 tgatcacttc tttcgccgtc acggccttac cgatactgct gacccagcca cgaaacacat
    12661 ccaccgtgcc attcggaaaa cggattttat aggcccggac atcgccgctt tcaaaccagc
    12721 ctataagccc tttctggcct tcctctcccg gtttccaggc cagcgtaaaa ctggtatcac
    12781 ctgcagattt ctgcccctgc ccggtcgcgc tccagtccgc gtcttcatca tccaggtagt
    12841 tatcatcata ggattcagcc gtcatctcgc ccggcgtcag atccttcacc ttagccagtc
    12901 gctgccagtc atcgtctgac aacgggtttg cataagcatc agccttgccg ttgtaaaccc
    12961 acagagtggt accggcacct tttaccggct ccaggggatt tggtgttgcc atatcgtcct
    13021 cacatctcgt atgtaatgga ataagtcaga tccgcagagc tccataacgc catatcgtca
    13081 tcacgacgat actcatagcc ctgcgtaacc atcgtggtaa tcagtcctgc cagtgcaggg
    13141 atcgcagtca tcgccggata aatccggctt tccatccact gatcgagctc cgaatcaggt
    13201 acctgtgccg gtaaaaacac ctcaatatgc agtgtggccc gccaggtatc tgcatccagc
    13261 tcttcaccgg tatactctgc atccgtcaga taaaccgcga tcgcaggaaa atcctcttcg
    13321 tcaaaaacaa cggggcgacc atcaaacagc gtcgccccgt gttcatgctg ctcgagtgca
    13381 tccagcactg cggcacgaat gtcagtgtgt ttcatcgttt tatcgcaatc ctcagttgtt
    13441 gtttcagcgc gtatgccagt tctttaggca ggcgttcacg ccggatacgg tcaacattct
    13501 catcaaatgc ctgtttcagt ggggccgcca tcgggatttt caccacctga atgggaaggc
    13561 gattacgctt tttccttccc ttgtcgtcat tgccctcctc atatctggcc tggggaagac
    13621 gttgcataac atgccagcgc ccattattta atcgctggat aaatgcccgc tgataacgat
    13681 gctgaccggc tttgagtatg ctgttcggac gacgccccag cattctgatc cccagcttaa
    13741 tcacagggag atcaccgcgg ttaacgataa ttctggcatt cggatttctg accgtcgccc
    13801 gtttcagtct ggaccgttcc tttaccagtt tccgtctcac cctggtttcc cgggcaacct
    13861 gtgacgaaga ctgattaatc gccgttgtgg ccacgcggtt aatggtcatt gcagaagcag
    13921 ccggaatggc gtttttacga acccggttca gattgtcaat cgcctgatca agccctttta
    13981 tcgccataat tcaccctgcg tttatcgtcg ccggttaact gcgggtggtt gcccacggtt
    14041 gagccagaga taacagctgc ccccgtcatc cggag
//
LOCUS       DAQSWQ010000112        14001 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.113, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000112 DAQSWQ010000000
VERSION     DAQSWQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.113"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="R8G18_003413"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="R8G18_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HEE9797025.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="R8G18_003414"
     CDS             2273..2662
                     /locus_tag="R8G18_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEE9797026.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="R8G18_003415"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="R8G18_003415"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEE9797027.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="R8G18_003416"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="R8G18_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HEE9797028.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="R8G18_003417"
     CDS             complement(4321..4521)
                     /locus_tag="R8G18_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HEE9797029.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="R8G18_003418"
     CDS             4687..5232
                     /locus_tag="R8G18_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEE9797030.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="R8G18_003419"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="R8G18_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="R8G18_003420"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="R8G18_003420"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HEE9797031.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="R8G18_003421"
     CDS             complement(6529..7353)
                     /locus_tag="R8G18_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HEE9797032.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="R8G18_003422"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="R8G18_003422"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEE9797033.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="R8G18_003423"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="R8G18_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEE9797034.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="R8G18_003424"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="R8G18_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HEE9797035.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="R8G18_003425"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="R8G18_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HEE9797036.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="R8G18_003426"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="R8G18_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HEE9797037.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="R8G18_003427"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="R8G18_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HEE9797038.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="R8G18_003428"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="R8G18_003428"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEE9797039.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3401 a         3545 c         3717 g         3338 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa t
//
LOCUS       DAQSWQ010000113        13902 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.114, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000113 DAQSWQ010000000
VERSION     DAQSWQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.114"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            241..1494
                     /gene="kch"
                     /locus_tag="R8G18_003429"
     CDS             241..1494
                     /gene="kch"
                     /locus_tag="R8G18_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HEE9797040.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1549..1722)
                     /locus_tag="R8G18_003430"
     CDS             complement(1549..1722)
                     /locus_tag="R8G18_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HEE9797041.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1865..3325
                     /gene="cls"
                     /locus_tag="R8G18_003431"
     CDS             1865..3325
                     /gene="cls"
                     /locus_tag="R8G18_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEE9797042.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3360..3689
                     /locus_tag="R8G18_003432"
     CDS             3360..3689
                     /locus_tag="R8G18_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HEE9797043.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="R8G18_003433"
     CDS             complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="R8G18_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HEE9797044.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="R8G18_003434"
     CDS             complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="R8G18_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HEE9797045.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="R8G18_003435"
     CDS             complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="R8G18_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HEE9797046.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="R8G18_003436"
     CDS             complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="R8G18_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HEE9797047.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="R8G18_003437"
     CDS             complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="R8G18_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HEE9797048.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="R8G18_003438"
     CDS             complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="R8G18_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HEE9797049.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11191..13866
                     /gene="adhE"
                     /locus_tag="R8G18_003439"
                     /gene_synonym="adhC"
     CDS             11191..13866
                     /gene="adhE"
                     /locus_tag="R8G18_003439"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEE9797050.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3656 a         3436 c         3231 g         3579 t
ORIGIN      
        1 ttatagccgg tcagtgcatt cactaccaca agcagataat aactttatct gtatttcttt
       61 atatgatgtg agcatattca ggaaaaatga cctttcctga ggtacagttg ctcgctcaaa
      121 tcctttgatt actgcggata aagtcatcta ctattgttta acacattcac gcgctgcatg
      181 tatgttcaga aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca
      241 gtgagtcact gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac
      301 gacattctcg cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt
      361 atgtcggtca atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc
      421 ttgctggcaa acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc
      481 ttactgttcc gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta
      541 atttacaccc tgcattttta tccctggctg aaatttagta ttggattttg catttttacg
      601 ctggtgtttt tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca
      661 atttttgcat ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat
      721 ttaagcgaag gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata
      781 gaaaccatgt caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg
      841 ttcactattt cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt
      901 tttggtccgc ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg
      961 catcgtaaag atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg
     1021 caactgaatc aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc
     1081 aagcaacttg agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac
     1141 agttcagtat taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat
     1201 aacgatgcag ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa
     1261 acagttctcg ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat
     1321 atcattctct caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa
     1381 gagattaata atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc
     1441 gataacgatg aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta
     1501 aagacgaaag agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta
     1561 gatcgcaaac aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat
     1621 acgcatattt cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg
     1681 ctgcatgcgc cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa
     1741 taacagaatg gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt
     1801 atttgccaga tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta
     1861 atctatgaca accgtttata cgttggtgag ttggttggcc attctgggat actggttgct
     1921 cattgcaggc gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc
     1981 ctggctgttg attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg
     2041 cgagctccat ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc
     2101 aaaatggctt aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt
     2161 cgctgcgcca ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa
     2221 tcagctacaa ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca
     2281 gctcgcgcgc cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga
     2341 ccaggtggct gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct
     2401 cgactccgcc gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc
     2461 cggtattgaa gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat
     2521 ggacctgcgc caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag
     2581 catgaatatg gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga
     2641 tctgatggcg cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga
     2701 ctgggagatt gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc
     2761 gtttgaacag gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga
     2821 agatctcatt caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat
     2881 gacaacgccc tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca
     2941 gcgcggggtg gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg
     3001 ggccagtcgc gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga
     3061 aggcgggtta ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac
     3121 cgttaacctt gatatgcgta gtctgtggct gaatttcgag attaccctgg caatcgacga
     3181 taaaggtttt ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct
     3241 gctcgatgcc cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt
     3301 ttacttcttc agcccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta
     3361 tggatatgga tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata
     3421 tttttctcga actggctgcc gacaatctcg acccagctga tgttctgctg ttcaatcttc
     3481 agtttgaaga gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg
     3541 ttgatttcga tttgaacccg gacttttttg ccgaagtggt gattggtctg gcggacagtg
     3601 aggatggtga aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca
     3661 aactttgcca tatcatctgg cgggaataaa taatcgcttt cggcgtgaaa aagggctgac
     3721 aactgtcagc ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct
     3781 gaaactccct tccagcacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaaca
     3841 ggtacggaaa acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa
     3901 ctggatggtt ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc
     3961 cctggtgtaa ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc
     4021 atggccgaga tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg
     4081 agcgataaaa attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac
     4141 ctgcgcctga attgacacgt ccagcgccga aatcggctca tcgcagataa tcagcttcgg
     4201 ttcaagaata agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg
     4261 cggatagcgg ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg
     4321 ctcgcgaact tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat
     4381 ctcgccgatg gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat
     4441 atcactgcga acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca
     4501 ggcaacatga ccgtcggtcg ctttaaccag gccaatgatg gcgcgagcaa aggtggactt
     4561 accgcatccc gattccccta ccacacctaa tgtttcccct tcatacaggc gaagcgtgac
     4621 accatcgacg gctttgagag ttttcggcgg ttgccagaac cactgtttgc catctttgat
     4681 ttcaaagtgc actttcagat cggcgatttc gaggaggact tttcttcctt cggttacagc
     4741 attcataaca gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct
     4801 tccagcggcg gtgcgctact acaaatttcc atcgcatgcg gacaacgtgg ctggaacggg
     4861 caacctttcg gtaatcgcag caggtttggc ggattaccag ggatggtcaa cattgtttca
     4921 ccttccgcat cgagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt
     4981 tgataaaaga catcgcgcgc gttgccatat tccatcgtgc gcccggcgta cattaccagc
     5041 actttgtcgc agatccccgc caccacgcca agatcgtggg taatcataat gatggcggta
     5101 ttaaattccc gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc
     5161 agcgcagtag tgggttcatc cgcaatcagc agcttaggtc gacatagcaa tgccatcgca
     5221 atcataactc gctgacgcat gccaccagaa aattcgtgcg ggtacatttt catgcgttta
     5281 cgtgcttccg gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct
     5341 ttgctcatgt tcttatgcag catcagcact tccatcaact gctcaccgac gcgcatatag
     5401 ggattcaacg aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta
     5461 ttgagttcac gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca
     5521 atacgtccgt tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa
     5581 ccagactcac ctacaatgcc cagcgtttct ccggcacgta gagaaaaatt caaatcattg
     5641 accgccgtga catcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc
     5701 agtgcgtcag cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct
     5761 gcactcctta acgatctttc gggtcgagag catcacgcaa gccatcgccg ataaagttga
     5821 aacaaaacag cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt
     5881 ccatcgagtt cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac
     5941 ccaaccccag gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg
     6001 cgtagaccac caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg
     6061 tcgatacacc gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt
     6121 gtccacgcac aatacgagcc atatccagcc aggaaaccat gccaatcgcc acgaaaatca
     6181 gcaggatgtt ttgaccgaaa aaggtcacaa gcaaaatgac gaagaacatg aatgggaagg
     6241 agttgaggat ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg
     6301 aaagcgaacc gtaaagtgtc cccacgacca ctgccaccag tgccgcagca acaccgacca
     6361 tgagtgagat acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag
     6421 taccaaagta gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat
     6481 cgtcataggc aaactgcgaa agcatcggtg ccaggattac aaataacgcg atcagcacca
     6541 gcactatcag actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc
     6601 acaagctgcg cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt
     6661 tcttacttaa catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc
     6721 atatagcaca tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag
     6781 gcttaacact aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc
     6841 cggcaaacca taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg
     6901 gcccatataa gagagcacgg gtaacagagc aggttttaat gcgtggcgta aaatgatccg
     6961 ccgcataggt aaccctttcg cccgggcagt acgaataaag ttggagtgta atacttcaat
     7021 catagagcct cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat
     7081 cggcaatatc atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa
     7141 aatgatcgca aatatcatga ctaataatgg cgcaaccaca aaactgggga taacaacccc
     7201 ggtcattgcc agccccatca cggtatagtc ccatttggtg ttttgtttta atgcgacaat
     7261 aacgccagca ctaacaccca gtattaccgc aaggaaaaat gcggcggctc ccagtttggc
     7321 agaaacggga aaactggatg caaccaggtc attgaccgaa taatctttat atttaaacga
     7381 tggaccgaaa tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg
     7441 atcattaaga tgatatttcg cttcgatatt ggccatcact tccggcggta aagtacgttc
     7501 gccggtaaaa gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat
     7561 aaatagcgtc ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc
     7621 ttctggcctg tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct
     7681 gccccacgta ttgccattag tgcttcacaa tgtacatatt ccgggtgtag gtattatcca
     7741 gcggatcttt gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt
     7801 aataaacagg aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag
     7861 ctgtgcgctg cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg
     7921 ggctcttata atgcgcggta ttcatcgagc tgttcgacag catggtgttc aggaaggaag
     7981 ttggttcgtt gtagtctgca caccagcctg cacgggccac atcaaaagtg ccctggtgac
     8041 gggtgtcgag gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt
     8101 tcttccacaa tgaagaggca gcaatcgcca gctttttatg cagatcagag gtgttataca
     8161 gcaggttgat ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg
     8221 cttcttcgtt acgtttttcc tggctccagc caaaccattc tggctgagtc aattttgcgc
     8281 catcagtata cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat
     8341 taacaatgat atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga
     8401 atggcggttt ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa
     8461 cttcgtccgg gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttatacg
     8521 tcatgtcgat ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca
     8581 aataggttac ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa
     8641 gaacgattcg ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat
     8701 taccaggctg ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag
     8761 ttgatgggtg aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta
     8821 atgtgtgatc atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa
     8881 tttcatcaat accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt
     8941 tcggatcaac agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgctat
     9001 cagaccattt cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc
     9061 aggattcagc gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa
     9121 acaggtctcg gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact
     9181 gaacttctga accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg
     9241 gtacatcagc tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta
     9301 ctaaacttct cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg
     9361 tattaaccag catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct
     9421 gtcaggttac tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag
     9481 acgatcacct tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc
     9541 gacgctcttt aattgttaat tattctcatt accaataaag aatcgtctgg cggttgcccg
     9601 gaaagtacca aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct
     9661 cttttattga ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa
     9721 ttcaatcaaa tagagattgg ctaagcatgt ctatcttttc tgattacagt tccagtagtg
     9781 aaatgcacaa taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg
     9841 gaattactaa tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa
     9901 taacatgatg gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa
     9961 gaatattttt acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat
    10021 gacgaagtaa acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc
    10081 ggggaaaatc cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag
    10141 ccccataata cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg
    10201 tgccatgcgg aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc
    10261 aaacagatag ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc
    10321 tggccccgcc atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt
    10381 ttctgatttt tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat
    10441 cgctatcgtc accaccagga tgccccctgc gatacggaac gaatcaattg atataccaaa
    10501 aagttgcaga atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag
    10561 gttggctgta aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat
    10621 aaagacggga ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa
    10681 gtaaacggga aaatccaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata
    10741 atgttagcca taaattaagg ttgaaaagac gcgctgacaa tacgcctttt gacagcattt
    10801 ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt tgtgcaaaac
    10861 atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat aatggcgaaa
    10921 agcgatgctg aaaggtgtca gcttagcaaa aatttgattt ggatcacgta atcagtaccc
    10981 agaagtgagt aatcttgctt acgccaccta gaagtgacgc attagagata ataactctaa
    11041 tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt ttgattttca
    11101 taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag atgatttact
    11161 aaaaaagttt aacattatca ggagagcatt atggctgtta ctaatgtcgc tgaacttaac
    11221 gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa
    11281 gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat cccactcgcg
    11341 aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac
    11401 tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct
    11461 gaagacgaca cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc
    11521 gttccgacca ctaacccgac ttcaactgct atcttcaaat cgctgatcag tctgaagacc
    11581 cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag atgccaccaa caaagcggct
    11641 gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga aagatctgat cggctggatc
    11701 gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat caacctgatc
    11761 ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc
    11821 ggtgtaggcg cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca
    11881 gttgcatctg tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag
    11941 tctgttgttg ttgttgactc tgtttatgac gctgtacgtg aacgttttgc aacccacggc
    12001 ggctatctgt tgcagggtaa agagctgaaa gctgttcagg atgttatcct gaaaaacggt
    12061 gcgctgaacg cggctatcgt tggtcagcca gcctataaaa ttgctgaact ggcaggcttc
    12121 tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa
    12181 ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa
    12241 gacgcggtag aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc
    12301 ctgtacactg accaggataa ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa
    12361 acggctcgta tcctgattaa caccccagcg tctcagggtg gtatcggtga cctgtataac
    12421 ttcaaactcg caccttccct gactctgggt tgtggttcct gggggggtaa ctccatctct
    12481 gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac
    12541 atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct gccaatcgcg
    12601 ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg
    12661 ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact
    12721 gaagtcttct tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa
    12781 ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc cccgatggac
    12841 gccgcgaaga ttatgtgggt tatgtacgaa catccggaaa ctcacttcga agagctggcg
    12901 ctgcgcttta tggatatccg taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg
    12961 aaaatgatcg ctgtcaccac cacttctggt acaggttctg aagtcactcc gtttgcggtt
    13021 gtaactgacg acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat
    13081 atggcgattg tcgacgccaa cctagttatg gacatgccga agtccctgtg tgctttcggt
    13141 ggtctggacg cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc
    13201 tctgatggtc aggctctgca ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac
    13261 cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc aactatcgcg
    13321 ggtatcgcat ttgcgaacgc cttcctgggt gtatgtcact caatggcgca caaactgggt
    13381 tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa cgttattcgc
    13441 tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag
    13501 gctcgccgtc gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt
    13561 actgctgcta agatcgagaa actgctggca tggctggaaa cgctgaaagc tgaactgggt
    13621 attccgaaat ctatccgtga agctggcgtt caggaagcag acttcctggc gaacgtggat
    13681 aaactgtctg aagatgcatt cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg
    13741 atctccgagc tgaaacagat cctgctggat acctactacg gtcgtgatta tgtagaaggt
    13801 gaaactgcag cgaaaaaaga agccgctccg gctaaagctg agaaaaaagc gaaaaaatcc
    13861 gcttaatcag tagcgctgtc tggcaatata aacggcccct tc
//
LOCUS       DAQSWQ010000114        13852 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.115, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000114 DAQSWQ010000000
VERSION     DAQSWQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.115"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(1..736)
                     /gene="dsbG"
                     /locus_tag="R8G18_003440"
                     /pseudo
     CDS             complement(1..736)
                     /gene="dsbG"
                     /locus_tag="R8G18_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1108..1671
                     /gene="ahpC"
                     /locus_tag="R8G18_003441"
     CDS             1108..1671
                     /gene="ahpC"
                     /locus_tag="R8G18_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEE9797051.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1842..3407
                     /gene="ahpF"
                     /locus_tag="R8G18_003442"
     CDS             1842..3407
                     /gene="ahpF"
                     /locus_tag="R8G18_003442"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEE9797052.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="R8G18_003443"
     CDS             complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="R8G18_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HEE9797053.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4177..5415
                     /locus_tag="R8G18_003444"
     CDS             4177..5415
                     /locus_tag="R8G18_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HEE9797054.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="R8G18_003445"
     CDS             complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="R8G18_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HEE9797055.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6286..7092)
                     /gene="rna"
                     /locus_tag="R8G18_003446"
     CDS             complement(6286..7092)
                     /gene="rna"
                     /locus_tag="R8G18_003446"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HEE9797056.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7206..8669)
                     /gene="citT"
                     /locus_tag="R8G18_003447"
     CDS             complement(7206..8669)
                     /gene="citT"
                     /locus_tag="R8G18_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HEE9797057.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8720..9598)
                     /gene="citG"
                     /locus_tag="R8G18_003448"
     CDS             complement(8720..9598)
                     /gene="citG"
                     /locus_tag="R8G18_003448"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEE9797058.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9573..10124)
                     /gene="citX"
                     /locus_tag="R8G18_003449"
     CDS             complement(9573..10124)
                     /gene="citX"
                     /locus_tag="R8G18_003449"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEE9797059.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10128..11660)
                     /gene="citF"
                     /locus_tag="R8G18_003450"
     CDS             complement(10128..11660)
                     /gene="citF"
                     /locus_tag="R8G18_003450"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEE9797060.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11671..12579)
                     /gene="citE"
                     /locus_tag="R8G18_003451"
     CDS             complement(11671..12579)
                     /gene="citE"
                     /locus_tag="R8G18_003451"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEE9797061.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12576..12872)
                     /gene="citD"
                     /locus_tag="R8G18_003452"
     CDS             complement(12576..12872)
                     /gene="citD"
                     /locus_tag="R8G18_003452"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEE9797062.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12887..>13852)
                     /gene="citC"
                     /locus_tag="R8G18_003453"
     CDS             complement(12887..>13852)
                     /gene="citC"
                     /locus_tag="R8G18_003453"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEE9797063.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3400 a         3506 c         3687 g         3259 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac acttttattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtagg tagatggtga cgcccatatc ctgatacttt ccgagataac
      601 ctttcattcc tccgggggca tcgaacgttt tgatgattgt aatgccctgt ttttcaatcg
      661 cttttactgg ggaaggaagt tcctctgcga aggcgattgc aggaagcaga gccagtaaaa
      721 gtatcttttt taacattaat ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat
      781 aacctatcac tgtcataggt aagagcttag atcaggtgat tgccctttgt ttatgagggt
      841 gttgtaatcc atgtcgttgt tgcatttgta agggcaacac ctcagcctgc aggcaggcgc
      901 tgaagatacc aaagggtagt tcagattaca cggtcacctg gaaagggggc cattttactt
      961 tttatcgccg ctggcggtgc aaagttcaca aagttgtctt acgaaggttg taaggtaaaa
     1021 cttatcgatt tgataatgga aacgcattag ccgaatcggc aaaaattggt taccttacat
     1081 ctcatcgaaa acacggagga agtatagatg tccttgatta acaccaaaat taaacctttt
     1141 aaaaaccagg cattcaaaaa cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc
     1201 cgctggagcg tcttcttctt ctacccggct gactttactt tcgtatgccc gaccgaactg
     1261 ggtgacgttg ctgaccacta cgaagaactg cagaaactgg gcgtagacgt atacgcagta
     1321 tctaccgata ctcacttcac ccacaaagca tggcacagca gctctgaaac catcgctaaa
     1381 atcaaatatg cgatgatcgg cgacccgact ggcgccctga cccgtaactt cgacaacatg
     1441 cgtgaagatg aaggtctggc tgaccgtgcg accttcgttg ttgacccgca gggtatcatc
     1501 caggcaatcg aagttaccgc tgaaggcatt ggccgtgacg cgtctgatct gctgcgtaaa
     1561 atcaaagcag ctcagtacgt agcttctcac ccaggtgaag tttgcccggc taaatggaaa
     1621 gaaggtgaag caactctggc tccgtctctg gacctggttg gtaaaatcta aatttccttc
     1681 gtctttcgcg ctaccaactc cggcgcgaaa tacttcggaa attcacctaa ttcttcgggt
     1741 gctgcggcac ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgtag
     1801 aggccgcttg catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga
     1861 aaactcaact caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc
     1921 tggatgacag cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt
     1981 cagacaaagt cacctttaaa gaagataaca gcttgccggt gcgtaagccg tctttcctga
     2041 tcaccaaccc aggttccaac caggggccac gttttgcagg ctccccgctg ggccacgagt
     2101 tcacctcgct ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt
     2161 ctctgctgga gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc
     2221 tctcttgcca taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc
     2281 cgcgcatcaa gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca
     2341 acgtgatggg cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga
     2401 cgttgactga aatcgttgcc aaaattgata cgggcgcgga aaaacgtgcg gcagaagagc
     2461 tgaacaagcg tgatgcttat gacgtattaa tcgtcggttc cggcccggcg ggtgcagcgg
     2521 cagcaattta ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg
     2581 gtcagatcct cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc
     2641 agaagctggc aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca
     2701 gccagagcgc cagcaaactg atcccagcag cagttgaagg tggtctgcat cagattgaaa
     2761 cagcttctgg cgcggtactg aaagcacgca gcattatcgt ggcgaccggt gcaaaatggc
     2821 gcaacatgaa cgttccgggc gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc
     2881 actgcgacgg cccgctgttt aaaggtaaac gcgtagcggt tatcggcggc ggtaactccg
     2941 gcgtggaagc ggcaattgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg
     3001 cgccagaaat gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg
     3061 acattattct gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc
     3121 tggaatatcg agatcgtgtc agcggcgata ttcacaacat cgaactggcc ggtattttcg
     3181 tgcagattgg tctgctgccg aacaccaact ggctcgaagg cgcagtcgaa cgtaaccgca
     3241 tgggtgagat tatcattgat gcgaaatgcg aaaccaacgt caaaggcgta ttcgcagcgg
     3301 gtgactgtac gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag
     3361 cctctttgag tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat
     3421 tcacctgcaa ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa
     3481 gccctgccgg gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaatgacc
     3541 agtacaggca gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc
     3601 gaaatcgatg ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac
     3661 tcattgactt catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg
     3721 atggtgaagt ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct
     3781 tcaaaacgac gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt
     3841 acgtgaagta gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct
     3901 ttgtcgctca attccatttc aaatacatca actggcataa tgattgtctt atacataacc
     3961 ctttctccct gttaatcatg aacaaatcat tcgccatgat tataatattt atccctgata
     4021 tttgtctggt tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac
     4081 caggaattct cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa
     4141 agactgactc ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc
     4201 ccacatcacg ttcaggtaga aaatgttccc gatccgggca ttgaacaggc tgatgatatt
     4261 attctgcgta ttacggcaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa
     4321 ataccccagg ttaaacatgg cgatattttg ggtcatgaat ttatggggga agtcgttgaa
     4381 accggaaagg acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gtttgtcatt
     4441 gcttgtggcg actgtttttt ctgtcgaatg cagcaatatg ccgcctgcga aaataccaat
     4501 gcgggtaaag gcgctgcgct caataaaaaa cagataccag ctcccgcggc attgtttggt
     4561 tatagtcacc tgtatggtgg cgttcctggt ggacaggcgg aatatgtccg cgtccctaaa
     4621 gggaatgtgg ggccgtttaa agtaccgcct ttgctttcag atgataaagc ccttttcctt
     4681 tctgatattc tgccaacggc atggcaggca gcaaaaaatg cgcagatcca acaaggttca
     4741 agcgttgcag tctatggtgc cggtccagtg ggattgttga caatcgcctg tgcacggttg
     4801 ctcggtgcgg aacagatttt tgttgttgat catcatccct accgcttgcg tttcgccgct
     4861 gaccgctacg gcgcgatccc gattaatttc gatgaagata gcgatccggc acagtcaatt
     4921 attgaacaaa cggcaggtca ccggggcgtg gatgcagtaa tagacgccgt cggttttgaa
     4981 gcgaaaggca gcaccacgga aacggtgctg actaacctga aactggaggg cagcagcggt
     5041 aaagcgttgc gccagtgtat tgcggcggtc aggcgtggcg gcattgttag cgtaccgggc
     5101 gtctacgctg gatttattca cggtttcctg tttggcgacg cctttgataa agggttgacg
     5161 tttaaaatgg gacagaccca cgttcacgca tggctgggtg aattattacc gttaattgag
     5221 aaaggattac tgaaaccaga agaaattgtt acccactata tgccgtttga agaggccgcc
     5281 cggggatatg agattttcga aaaacgtgaa gaggagtgcc gtaaggtgat tctggtaccc
     5341 ggtgcacaaa gcgcagaggc ggcgcagaag gcggtttcag gtctggtgaa tgcgatgccg
     5401 gggggaacaa tatgatcgtc aggagtggtt ttcgaggtaa aggacagcca tgacgataat
     5461 cgccgccata atcagaaatc ctatcaggat gtaaaatgct tctgccatgg ttattcccac
     5521 aaacgaaacg gaataatttt gcagcaaaag tgaacagtga gaaccaggaa aaattgctga
     5581 ttttgcgtaa agaggatgcg agtgcatcct ctgggcaaag cgagttatcg cttgtgcaga
     5641 tgggattaaa gcaggtagtc gccagcagct tctggctggt attcgagttc cagcacttca
     5701 aggtgggttg caacgccgcc cggaagttcc cagtgaatgg aatcgccaac gcgcagcccc
     5761 agcagtgcgg cacctaccgg agccataacg gaaagctgag tattgctatc ggtcattttt
     5821 gccggataca ccagcgtgcg cacacgcact tcgccatcgc taagattgcg gaatttaacc
     5881 cggctgttca ttgtcaccac gtcgtgtggc atctcttctg gcgaacacat ttgggcgcga
     5941 tccaactctg cgtttaacgc gtcggcgatt ggcaaaccag cataggcggg ttgctccagc
     6001 agaatatcga tgcgttcggc atccaggtcg ttaatgatga tagttggtct ggacattttt
     6061 actccatgtc gtcggtactg cgagtgtcgc agataaacat acccaaaaga aaaccctcac
     6121 cgtcaggcgg cgagggttta actcacatga tgatactgac tgttgctcac tctttgaagt
     6181 gatttgcgtc acattcaggg aattcctcaa tgcagcgcat tatgtataat tcttaatcgt
     6241 cacggtttat ggaagacgaa ttagcgtgtt ttgtaaatca gatgattaat aacccacttt
     6301 atcaatcaca aaggttttgc cacagttgcc tgggtgtggt tgtggcaaga atgagtttgc
     6361 agaaagcgga gcgttgatgg cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc
     6421 agggttaccc tggcacgtta gcttaactgc tttcacgttc tcttttcccc agcttttggc
     6481 aaaggcggcg tcaaagtcac ggcggctcac tgttttaccg taattatccg caaggaattt
     6541 tccggcttcg ctctctttaa tttcttgatt caggcgtacc atcgtaccga agtatgcgtc
     6601 cggatcaaaa ccaaagcagg caccgtgttt ggcatattcg tagcgttcca ggcaggaacg
     6661 tccgccagct cctggcatga cttcacttag tttagcggcc gtttccagtg ataacccggt
     6721 ttccggcgat gaacacatcc ggctggcgtg cgcttctggt agattcggga ttgggcgagt
     6781 agcgcaaccg aagcgcatcc agcggcgttc atcaacacca cgggcagcaa ccgatttagg
     6841 caatcctggc cacagaccat gtacggtcag aaaatcagct ttgttggtcg tttcggtttg
     6901 taggcgacat tcatctcgtt cgttacgatt tcgatcgtgt tgactctggc aaaatccggt
     6961 ttgccaggag agggccagga catagcgatc aaaatcgcca tactgttttg cctgcaacgc
     7021 tacggcgttg gcagaagaga agggaagcag agaaaccgcg agcaacgcgg cgttacgcca
     7081 gaatgctttc ataatgggtg tggaactcat acatacactg aatactatct attaaatcat
     7141 aaaaaagccc gccaggtggg cttacctggc gggcgtgatg atttattcag cgtttggcga
     7201 acgtattagt tccacatggc gagaatcggc cagccaacca acagcaacat agagatgtaa
     7261 atcaccccga agattgcgcc aagacgccag taatcttttg atttcacata gccacagccg
     7321 taaataatca ccccaggacc ggttgcatac ggcgtcagac agcccatgat accgatagac
     7381 agcaccagca ggatacacag ttgttccatt ggtacgcccg gaataccttt accgacggcc
     7441 agaataaccg acagcatggt tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca
     7501 aagtagaaca ccagaaccag tacaatcacc gttgcgtttg gtgagaatcc ttccaggtgc
     7561 gtactcatgg taccggcgaa ccagtcaata aagccagaac gagtcaggcc gttagccatc
     7621 acaaccagag ttgccaggtt gaccagcgtg ttccatgcgc tgttatagcg ggtaatatct
     7681 ttccaaggca caacgtgcag ggccagcatt agcgaaactg ccagcagacc aactgcagta
     7741 gcattaatga cttcactgcc aaaaacccac aaacctaagc tgagcaatac aaggccaatc
     7801 agtgtccact ctctgcgtgt cagcgcaccc atggttttca gttcatcacc cgcccaggtt
     7861 gccacttctt cactgtgtgt gatttccggt ttgtacagca cgtaggaaag ccacggcgca
     7921 atgataagca agataacccc aaccggcagg aagcagagga accactgcaa ccagctaatc
     7981 tggataccgg caattttgct gacgaactcc agacccagca cgtttggtgc cgcaccggtg
     8041 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     8101 atacgacgcg cggacggatc gttggggaat gatttaaaca gcggcggcag gtttttaatg
     8161 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     8221 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgcccat gaatttcacc
     8281 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     8341 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccagacccca tttcagcgcc
     8401 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     8461 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     8521 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgtggtggc
     8581 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     8641 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     8701 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     8761 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     8821 cgagatcggc gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     8881 gccagcgcag gcccccctcg ccaccgcgcg atgcaacgtt ggtatcgccg ttgatcgcca
     8941 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     9001 tgaggtaatg cggcaaggcg tgattgatca ccagtggata acccgcttcg gcttcaccgc
     9061 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt tgtgaattat
     9121 tggtacgcag ttctcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     9181 ttgttggcgt taccggttgg ttgagttgaa gcaaacggcc aattgccgca catagtagcc
     9241 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     9301 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttctggt gctatttccg
     9361 cactacaggc accaaattca atgaaacggg gtagccagcc ctgaatcgcc agcgcgctgc
     9421 ggtggaaatc ttccagcgcc atatctttat gtgcaccgca gttaatccga tccacgaggc
     9481 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     9541 catcgatcaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     9601 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     9661 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     9721 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     9781 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     9841 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     9901 tgttttgcgg ctaaggcacg caaggctgtc acgccatgat taaaaattcg gcgtgtgacc
     9961 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
    10021 cgcttgagcc agacgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
    10081 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
    10141 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
    10201 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cgcgttcgcg cagccactca
    10261 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ccagttccgg acgtgccggg
    10321 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcctgg ggtgatgcag
    10381 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagcggcgc gacgatgatg
    10441 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcaccacg cagtacgccg
    10501 tcagagccgg tcagcacgtt aacgttgaac tgggtgtcaa tttccagcgc gctcagtacc
    10561 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
    10621 ctgatttcga tgtgattggg gttacgggcc agcgattgcg cagcatggct gtcaaagctc
    10681 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
    10741 ataccgccga gggcgaagtc ggcgcgaata tcgcggctac gcattttgtc ttccaggaaa
    10801 cgggttaccg ccagcgatgc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
    10861 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
    10921 gtggtcatac gggtcgcgcc agcgccgatt tttgcagcat cgccaacgcg gtcaactttg
    10981 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
    11041 tcttcggtaa gcatcacaac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
    11101 gagccgcagc aggctttacc ggtgtaaccg ttggcattac cgaattcatc acaggacggg
    11161 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgtccg
    11221 ccgtgagagt ggatttgcac cggttccgca agcagaccac gggaaatttc ttctgccagt
    11281 ggaccgcgca ggccggaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
    11341 agtggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
    11401 ttagcgatga cgtccatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
    11461 aaggaaaccg tcatgccgtc ctgtaaacca gagcgacgaa tcgcttcttc ctggttggcg
    11521 cacagtttgc gatcgcgcgc tttttcagcc tggtaggttt gctttggtga gttctggaaa
    11581 gcggcaagat cgcattcagc gcgacgattc caggccgcta cccgttcttg tcgttgagat
    11641 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
    11701 acgggagagc accagacggg cacgatcgat aaccggaccg tccaccatct tgccgttcag
    11761 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggcgggc
    11821 gtgatccact tctttctggg tcggtgcgta gaggttgtgc aacagatcaa tctgacgcgg
    11881 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
    11941 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
    12001 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
    12061 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
    12121 agcgtgagcg atttccactg cgcgggtaat ccccagcgga gattcaatcg ccgccagcag
    12181 gccggtgctg ccgggttcac gaccacaggc tttttcgata cgcaggatct ctttttcgat
    12241 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
    12301 aacgacggct tccaggtcgt tgacacccca ttcggaatcc agcgcgttga cacgcacaat
    12361 ggtttcaata tcgcgataca gcggatgttg cagcgcgtgg taaaccatgc ggcgggcggt
    12421 gtctttttca cgcaatgcta cggagtcttc gaggtcaaac atcagggcat cagccgggta
    12481 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
    12541 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
    12601 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcacgtaa aatgcagtcc
    12661 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
    12721 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
    12781 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
    12841 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
    12901 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
    12961 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
    13021 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
    13081 tgggcggtgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
    13141 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacaccc agcgcgggag
    13201 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
    13261 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattcg gagccgcgat
    13321 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccaac cggtcttcat
    13381 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtgccg
    13441 cagcttgttg aatcagataa cggtgaccat tcgtgaaggg attggcgttc atcacaatgc
    13501 agccaatctt gttccctgga tgacgaaatt ttttcagcga ttcggcatag cgtttcagcc
    13561 gcgtggcgct gttttccatc agcaccatca cgccgggtac gctggtcagc gtggaaaaac
    13621 cgcactggcg gaacagcgcc tcgtattcgg ttttggtata aataaacaga tgcgtgctgt
    13681 gccgctcata ggcgaggttt atcaattcag tggctaatgt cagcgccagt ccttcaccgc
    13741 gaacggattc actgatagca acgcatttaa taatatttcc ggcaattcca ccgcacgtga
    13801 taagcttttc atcgcgggtt acggtaataa atacttcgac tgtggtgtca ac
//
LOCUS       DAQSWQ010000115        13773 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.116, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000115 DAQSWQ010000000
VERSION     DAQSWQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.116"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(894..1985)
                     /gene="ychF"
                     /locus_tag="R8G18_003454"
     CDS             complement(894..1985)
                     /gene="ychF"
                     /locus_tag="R8G18_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEE9797064.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2102..2686)
                     /gene="pth"
                     /locus_tag="R8G18_003455"
     CDS             complement(2102..2686)
                     /gene="pth"
                     /locus_tag="R8G18_003455"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEE9797065.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2963..3241
                     /gene="ychH"
                     /locus_tag="R8G18_003456"
     CDS             2963..3241
                     /gene="ychH"
                     /locus_tag="R8G18_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HEE9797066.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="R8G18_003457"
     CDS             complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="R8G18_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HEE9797067.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5099..6046)
                     /gene="prs"
                     /locus_tag="R8G18_003458"
     CDS             complement(5099..6046)
                     /gene="prs"
                     /locus_tag="R8G18_003458"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEE9797068.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="R8G18_003459"
     CDS             complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="R8G18_003459"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEE9797069.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="R8G18_003460"
     CDS             complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="R8G18_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HEE9797070.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7885..9141
                     /gene="hemA"
                     /locus_tag="R8G18_003461"
     CDS             7885..9141
                     /gene="hemA"
                     /locus_tag="R8G18_003461"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEE9797071.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9183..10265
                     /gene="prfA"
                     /locus_tag="R8G18_003462"
     CDS             9183..10265
                     /gene="prfA"
                     /locus_tag="R8G18_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEE9797072.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10265..11098
                     /gene="prmC"
                     /locus_tag="R8G18_003463"
     CDS             10265..11098
                     /gene="prmC"
                     /locus_tag="R8G18_003463"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEE9797073.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11095..11487
                     /locus_tag="R8G18_003464"
     CDS             11095..11487
                     /locus_tag="R8G18_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HEE9797074.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11491..12300
                     /locus_tag="R8G18_003465"
     CDS             11491..12300
                     /locus_tag="R8G18_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEE9797075.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12336..13190
                     /gene="kdsA"
                     /locus_tag="R8G18_003466"
     CDS             12336..13190
                     /gene="kdsA"
                     /locus_tag="R8G18_003466"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEE9797076.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13339..13446)
                     /locus_tag="R8G18_003467"
     CDS             complement(13339..13446)
                     /locus_tag="R8G18_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HEE9797077.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3452 a         3428 c         3515 g         3378 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      361 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat
      421 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      481 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      541 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      601 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      661 tgtgtagttg aacaaaaact tacgttatat cactactaat gagatatccg tcatctgaca
      721 ctgatgtctt taattattct cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg
      781 ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata
      841 tacttttgcg ctcttcatga gatttagcga aacctcatga gacaataaat taattagacg
      901 ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc
      961 attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag
     1021 atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc
     1081 ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag
     1141 taagtttgca ggttcagcag cttataaccg acacggatca cacggttcag gcccggttct
     1201 tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct
     1261 gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc gatttcacgc
     1321 acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt
     1381 gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa
     1441 tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc taggaccgcc
     1501 agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca
     1561 cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc aatatcgtca
     1621 gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg
     1681 tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct
     1741 ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta
     1801 cgttgcggtt ttacgatttc agccagttga tccaggcgag ggtcaggcat tggtacgacg
     1861 cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc
     1921 agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat
     1981 cccatgattt aaatcacctt aatatcttaa taatcaatct gttattgcta acagattgca
     2041 gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga
     2101 cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc
     2161 acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa
     2221 caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgccagga tgaccgattc
     2281 cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc
     2341 cattgtgacc accatggcca ccgcccaatt taaatttggc gacgccagga ggcagatcca
     2401 gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg
     2461 caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg acatcttcgc
     2521 ctccaagagt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc
     2581 gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt
     2641 attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt tttgtcctga
     2701 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2761 atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag
     2821 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2881 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2941 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     3001 catggggttg gggctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     3061 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     3121 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     3181 ctggtgggtt cgtcactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     3241 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     3301 aatccgccat cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa
     3361 tgcctgcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3421 atccttcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3481 ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3541 ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa
     3601 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3661 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3721 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca
     3781 agtcgaccac tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca
     3841 tagcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3901 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3961 aacgcgcgat ggcagctgta gcggtaatac caccaaagaa cggagcgata atattcccca
     4021 gtccctgtcc aaccagttcg ctgttcgcct tgtgtttcgt cccggtcata ccatccagta
     4081 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     4141 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     4201 gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     4261 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     4321 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     4381 aaaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4441 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4501 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4561 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4621 tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact
     4681 gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4741 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4801 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4861 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4921 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4981 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     5041 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc gggttcgatt
     5101 agtgttcgaa catggcagag atcgattctt cgttgctgat acgacgaatc gcttcggcca
     5161 gcatacctga cagggtcaga gtacgcacgt tcggcagtga tttgatttca tcgctcagcg
     5221 gaatggtatc gcagacaacg acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt
     5281 tgccagagaa gatcgggtga gtcgcgtacg caaatacacg tttagcacca cgttctttca
     5341 gagcttcagc agctttacac agcgtaccgc cagtgtcgat catatcatcg accagtacgc
     5401 agtcacgacc tgcaacgtca ccgatgatat gcatcacctg ggaaacgttc gcacgcggac
     5461 gacgtttgtc gatgattgcc atatcggtat cgttcagcag cttagcgata gcgcgggcac
     5521 gcacaacgcc gccgatgtcc ggagaaacca caattgggtt atccagattc agctgcagca
     5581 tgtcttccag caggatcggg ctaccaaata cgttatcaac cggaacgtcg aagaaaccct
     5641 gaatctgttc agcgtgcaga tccactgtca gcacacggtc aacaccgacg ctggagagga
     5701 agtctgcaac cactttcgca gtgattggta cacgagcgga acggacgcga cggtcctggc
     5761 gcgcatagcc aaagtagggg ataacagcgg tgatacgacc tgcggaagca cgacgcaggg
     5821 catcaaccat aacgactaat tccatcaggt tgtcgttagt aggggcacaa gtggactgga
     5881 tgatgaaaat atcaccaccg cgtacatttt cgttgatttg tacactgact tcgccgtcgc
     5941 taaagcgacc tacagcggcg tcgccgagtg aagtgtacag gcggttggca atacgttgtg
     6001 ctagttccgg ggtggcgtta ccagcaaaaa gcttcatatc aggcacgaga agaacctcag
     6061 gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg cgggccaggc atgatccttt
     6121 ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa
     6181 actcagcttg cccggcttaa agcatggctc tgtgcaatgg ggaaagatta acacctttcg
     6241 ccacaaagcc attgagccat tccggggctt gctctagcac ctggcgggct tcagactctg
     6301 tatcaaattc agcaaagaca caggcccctg tcccagtcag gcgcgacggg gcgtattcta
     6361 acagccagga aagcaccgca tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat
     6421 cattgctgaa ttcacatttt agcaacgttt ctattgacct ttttggcgta ttgcgcggga
     6481 gttcaggatc tttaaaaatc actggtgtcg gaatacttac accagggtgc gccaccagat
     6541 accacttctc tggcggatcc accggcgtta gtatttcacc aacgccttcg gcaaacgcgg
     6601 catgcccccg aacaaagaca ggaacatctg cgcccagcgt cagccccatt tccgccagct
     6661 catccatgct tagcccgcat tgccagagat gatttaatgc caccaggacc gtcgcggcat
     6721 tggatgaacc accgccgaga ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg
     6781 caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt tttcatcaac aaccgcgctg
     6841 cgcgaacgat caggttatct tcatgttcca cgccttcaac gggcgttaac agacgaatat
     6901 ccccatcgtc acgaagctca atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg
     6961 tttgcagcgt gtggtaacca tccgcacgct gaccggtaat gtataaaaac agattaagtt
     7021 ttgccggaga gggccactgt gtccgcatta tttcactatc cagttatcca tttttaactt
     7081 gatgcgttga ccaccgtcgg tgagttccat attggctggc atcgcaggtt gcgttttggt
     7141 gtcataacca ccataaacaa ccttccagtt tttgccattc tggctgtagg taatttcgct
     7201 caggcggtac tggtcgtcca gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca
     7261 ttggcgcaag ctgttgagcg gaattggcat tccggtcaat ttgccaatca tctcttcggc
     7321 gtcatcggcg gtataacgct gacctttatt gtcgactaac tgcacgttac ccggttgagc
     7381 attcagctcc agttccgtgc tgcccaatgg gttagtgagc agcagacggt agcgatcctg
     7441 gccggtttgc tgccagaaaa agcgggcgta cactttttgt tggtcagaaa tataagcgaa
     7501 cgcgccgcga gtctgatact gattaagatt gcgcacgtct tgctgatgct gacgccattg
     7561 tggcgaatcc gggcttttgc caggaccttt gggcgtggta acggaacagg cagtgagcac
     7621 aagagcagcc agcggtagca ggcggataag acgaaaatcg ggcaggggca tagtgatgac
     7681 aagtccttga gatacgttgc agttataacc cttaatgcta gcgttaccgt ccgctatcgt
     7741 ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt tcggcaatta ctccaaaagg
     7801 gggcgctctc ttttattgat cttacgcatc ctgtatgatg caagcagact aaccatatca
     7861 acgttggtat tatttcccgc agacatgacc cttttagcac tcggtatcaa ccataaaacg
     7921 gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt
     7981 gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc
     8041 acggaacttt atcttagcgt tgaagagcag gataacctgc aagaggcgtt aatccgctgg
     8101 ctttgcgatt atcacaatct taatgaagaa gatctgcgta aaagcctcta ctggcatcag
     8161 gataacgacg cggttagcca tttaatgcgt gttgccagcg gcctggattc actggttctt
     8221 ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat
     8281 atgaaggcca gcgaactgga acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt
     8341 cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg
     8401 gcgcggcaga tctttgaatc gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa
     8461 accatcgagc tggtagcgcg tcatctgcgc gaacataaag tacagaagat gattatcgcc
     8521 aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag ttggcgcgga agtgattgcc
     8581 ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca tcatcagttc caccgccagc
     8641 ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa
     8701 ccaatgctgt tggtggatat tgccgttccg cgcgatgttg agccggaagt tggcaaactg
     8761 gcgaatgctt atctttatag tgtggacgat ctgcaaagca tcatttcgca caacctggcg
     8821 cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg ctcaggaaac cagcgaattt
     8881 atggcgtggc tgcgagcaca aagcgccagc gaaaccattc gcgagtatcg cagccaggca
     8941 gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg cccttgagca gggcggcgac
     9001 gcgcaagcca ttatgcagga tctggcatgg aaactgacta accgcttgat ccatgcgcca
     9061 acgaaatcac ttcaacaggc cgcccgtgac ggggataacg aacgcctgaa tattctgcgc
     9121 gacagcctcg ggctggagta gcagtacatc attttctttt tttacagggt gcatttacgc
     9181 ctatgaagcc ttctatcgtt gccaaactgg aagccctgca tgaacgccat gaagaagttc
     9241 aggcgttgct gggtgacgcg caaactatcg ccgaccagga acgttttcgc gcattatcac
     9301 gcgaatatgc gcagttaagt gatgtttcgc gctgttttac cgactggcaa caggttcagg
     9361 aagatatcga aaccgcacag atgatgctcg atgatcctga aatgcgtgag atggcgcagg
     9421 atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga acagcaatta caggttctgt
     9481 tactgccaaa agatcctgat gacgaacgta acgccttcct cgaagtccga gccggaaccg
     9541 gcggcgacga agcggcgctg ttcgcgggcg atctgttccg tatgtacagc cgttatgccg
     9601 aagcccgccg ctggcgggta gaaatcatga gcgccagcga gggtgaacat ggtggttata
     9661 aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatccg
     9721 gcggtcatcg cgtgcagcgt gttcctgcta cggaatcgca gggtcgtatt catacttctg
     9781 cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga actgccggac atcaacccag
     9841 cagatttacg cattgatact ttccgctcgt caggggcggg tggtcagcac gttaacacca
     9901 ccgattcggc aattcgtatt actcacttgc cgaccgggat tgttgttgaa tgtcaggacg
     9961 aacgttcaca acataaaaac aaagctaaag cactttctgt acttggtgct cgtatccacg
    10021 ctgcagaaat ggcaaaacgg caacaggccg aagcgtctac ccgtcgtaac ctgctgggga
    10081 gtggcgatcg cagcgaccgt aaccgtactt acaacttccc gcaggggcgc gttaccgatc
    10141 accgcatcaa cctgacgctc taccgtctgg atgaagtgat ggaaggtaag ctggatatgc
    10201 tgattgaacc gattatccag gaacatcagg ccgaccaact ggcggcgttg tccgagcagg
    10261 aataatggaa tatcaatact ggttacgtga agcaataagc caacttcagg cgagcgaaag
    10321 cccacgccgt gacgctgaaa tcttattgga gtatgtcact ggcagggggc ggacttttat
    10381 tctggccttt ggtgaaacgc agctgactga cgaacaatgt cagcaacttg atgcgctact
    10441 gacacgtcgt cgcgatggtg aacccattgc tcatttaacc ggggtgcgag aattctggtc
    10501 gttgccgtta tttgtttcgc cagcgacctt aattccgcgc ccggatacgg agtgtctggt
    10561 ggagcaggca ctggcgcggt tgcctgaaca accttgccgt attctcgatc tcgggacggg
    10621 taccggggcg attgcgcttg cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt
    10681 agatcgtatg cctgatgctg tctccctggc acaacgtaat gcccagcatc tggcgatcaa
    10741 aaatatccac attctgcaaa gcgactggtt tagcgcgcta gccgggcagc agtttgcgat
    10801 gattgtcagc aatccgccgt atattgacga gcaggaccca catcttcaac aaggcgatgt
    10861 ccgctttgag ccgctcactg cgctggttgc ggcagacagt ggaatggcag acatcgtgca
    10921 tatcatcgaa cagtcgcgta acgcgctggt atccggcggc tttctgcttc tggaacatgg
    10981 ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc gcggggtatc atgacgtcga
    11041 aacctgccgt gactatggtg ataacgagcg cgtaacgctc ggccgctatt atcaatgaca
    11101 agtttttcta cactgcttag tgttcatctt attagtatcg cgctttctgt tgggctatta
    11161 accttacgtt tctggctacg ttatcagaag catccacagg catttgctcg ctggacgcgc
    11221 attgtgccgc cggttgtcga tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa
    11281 gcgcacatcc tgccattttc cgggcaggca cagtggctga ctgaaaagct gtttggagtt
    11341 atcatttata tcgttttggg ttttattgca ctcgattatc gtcgtatgca cagtcagcag
    11401 gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt acatcatcat taaactcgcc
    11461 accacaaaag taccgttact ggggtaagtc atgagatcgt tagctgattt cgaatttaat
    11521 aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag caatccgccg cgattttccc
    11581 tcgcaagatg tttacgacga actggagcgt ctcgttagtc tggcgaagga agaaatcagc
    11641 cagcttctgc ctttagaaga gcagctggaa aaactgatcg cgctgtttta cggcgactgg
    11701 ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg cattatggct ggaccaggtg
    11761 ttaaagaatc gacagggcag tgcggtatca ttaggtgcgg ttttattatg ggtcgcgaat
    11821 cgtctcgatt tgccgctgct gccggtgatt ttccctacgc agctgatatt gcgcattgaa
    11881 tgtccggatg gcgaaatttg gctgattaat ccttttaacg gtgaatcgtt aagcgaacat
    11941 atgctggacg tatggttaaa gggaaatatc agcccgtcgg cggaactgtt ttatgaagac
    12001 cttgatgaag ctgataacat tgaggtaatc cgcaaattgt tggatacact caaagcctcg
    12061 ttgatggaag aaaatcagat ggagctggcg ttacgcacca gcgaagcttt attacaattc
    12121 aacccggaag atccctatga aattcgcgat cgcgggttga tttatgcgca actggattgc
    12181 gaacacgttg cgttgaacga tttaagttat ttcgttgaac agtgtccgga agacccgatc
    12241 agcgaaatga tccgtgcgca aataaataac atcgcgcata aacatattgt gctgcattaa
    12301 ttaatcgaca ttttactcaa gattaaggcg atcctatgaa acaaaaagtg gttagcattg
    12361 gcgacatcaa cgtagcaaat gacctgtcgt tcgtactgtt tggtggtatg aacgtgctgg
    12421 aatctcgcga tctggcgatg cgcatttgcg agcactacgt aaccgtgacc cagaaactgg
    12481 gtatccctta cgtgttcaaa gcctcttttg acaaagccaa ccgctcctcc atccactctt
    12541 atcgtggacc gggcctggaa gaagggatga aaatcttcca ggagttgaag cagacttttg
    12601 gcgtgaaaat tatcaccgac gttcacgaac caagtcaggc acagcccgtt gctgatgtcg
    12661 tggatgtgat tcagttgccg gcgtttcttg ctcgccagac tgacctggtt gaagccatgg
    12721 cgaaaaccgg tgcggtaatt aacgtcaaga aaccacagtt tgtcagcccg ggacagatgg
    12781 gtaatatcgt tgataaattc aaagaaggcg gcaacgaaaa agtgattctt tgcgatcgcg
    12841 gtgctaactt cggctatgac aacctggttg tcgatatgct gggcttcagc attatgaaga
    12901 aagtgtctgg taactcgccg gtgattttcg acgtgaccca cgcactgcaa tgccgcgatc
    12961 cgtttggcgc agcttccggt ggtcgtcgtg ctcaggtggc tgagctggca cgagccggta
    13021 tggcggtagg tctggcgggg ctgtttattg aagcgcatcc ggatccggaa catgcgaaat
    13081 gtgatggtcc atccgcgctg ccgctggcta aactggaacc gttcctcaag cagatgaaag
    13141 cgattgatga tctggtgaaa ggtttcgaag aactggatac cagcaagtaa tcttttttgc
    13201 ttgaaaaata aagtcttagc gttctgcgtt aagacttttt tcatgggtgc cagatacaaa
    13261 aaaggccgca ggctgttacc cctgcggccg gtttcgggcg catattgcca tcacggcagc
    13321 ctgacgcccg ttttcacctt acttccggtt acgccaccag ccgacaatcg ctgcggtaat
    13381 aattcccgcc aggatcggtg ctgccaggtc gtgccagaaa atcatggcaa actgcgcgag
    13441 cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg ctattcttga attgttctgg
    13501 ttcaagatta gcccccgttc tgttgtcagg ttaaacctct caacgtgcgg gggttttctc
    13561 tttccagcaa ccaatgccac cagggataaa gcccccgcaa cattgcgcct caccggataa
    13621 cgccggcttg atgtggatac tacgtctcaa ttcatcttca cttcatccct gaaatgtttg
    13681 caatgaagag tgcattccgg tttttcaaca gctgttacag tcgtttcatg agtgctctgg
    13741 atgatgcttc cagctcgggt tgccaatatt tac
//
LOCUS       DAQSWQ010000116        13549 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.117, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000116 DAQSWQ010000000
VERSION     DAQSWQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.117"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..312)
                     /locus_tag="R8G18_003468"
     CDS             complement(<1..312)
                     /locus_tag="R8G18_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEE9797078.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="R8G18_003469"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="R8G18_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HEE9797079.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="R8G18_003470"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="R8G18_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HEE9797080.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="R8G18_003471"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="R8G18_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEE9797081.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="R8G18_003472"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="R8G18_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEE9797082.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="R8G18_003473"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="R8G18_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEE9797083.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="R8G18_003474"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="R8G18_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HEE9797084.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="R8G18_003475"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="R8G18_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEE9797085.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /locus_tag="R8G18_003476"
     CDS             complement(8292..9365)
                     /locus_tag="R8G18_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9797086.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..11898)
                     /locus_tag="R8G18_003477"
     CDS             complement(9376..11898)
                     /locus_tag="R8G18_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEE9797087.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(11923..12654)
                     /locus_tag="R8G18_003478"
     CDS             complement(11923..12654)
                     /locus_tag="R8G18_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HEE9797088.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12702..13274)
                     /locus_tag="R8G18_003479"
     CDS             complement(12702..13274)
                     /locus_tag="R8G18_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9797089.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3600 a         3419 c         3034 g         3496 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctagagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaagta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 cgtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg tccaaatcgc cacctgtact ggcggtgttg agatcggtga tctcaaaagc
    10981 accttgtgga acggcagact gataaatcac gtagccattc tgccgaatga tgacggtggc
    11041 gttggacttt gcaatcccac gaaccacagg agcaaagcca ttctggctgg cgggtagcat
    11101 atcgttatca gtatacaaac gcgcgccgcg aatttgcgta ctgtcgaaaa tatcgctcgc
    11161 cgtccacgta tcaccaatca taatctggct tcttaacgcg gcaatatcgc gctgtatcca
    11221 ggtactaatg gagttgtatt ccgcacggcc atcgctggtg tttaaagtac tgtaattacg
    11281 tagccgccac ggtccaatat tcagtccatt gcgcagattg agataactgg tatctgtctc
    11341 ttgtgaacca taatctgaac gtagtgtatg tgaaccagaa aaatcgtagt tgatggtgag
    11401 cgcgttaatc ccctcatccc aacgcgtggg gctgatgtaa tcataagcca tcgcgtccat
    11461 gtagatttgg ggaatggata tattcagctg caatttatca ctgtcaaact gccaggatga
    11521 gccttcaata cgtggagcag gatcgaggca aaccgtctca tccacatcat tgaacgattt
    11581 gagcgcatca acttttatgc caaaggagga cagtgttttt ttaatggcaa taaagcaagc
    11641 gtgcagatca gcatctggat tatcctgaac aaatgtcaca tcctgacgtt caaacatttt
    11701 accgttgata aaaatatcaa cgctataaac acctgggggc atcaggtttg cccgattaaa
    11761 ctggcgaata tcgactccgg attgatggtc gatttccaga agcgccggat tgaatacact
    11821 gacctcggcc atcaggtttt gcgagaaaat cagacacgtc cctgccgtga ggccaaccac
    11881 tattctggtc gtcatcataa tacgcctatc cttatccctt gcttacagcg tcatttttat
    11941 cgggtcactg actgcgccgt aatcattaat aaatcgccag gtcatttggc ctcccgcaga
    12001 attagcaggt aaaggtaacg tgagcgtttc aaaaggacgg atcatattga ggggcacatc
    12061 atggccatca acgatgacat ccatcacact gatatgaatt gggttgggat tctctgctat
    12121 cagatttcgc ccttcccggc gccatttcac tttcggcgca gcatcttcgg gaataaggcg
    12181 gatatttgct ggtcgccaga acagcttgat acgcgttttt actgctattt ccagcgagtt
    12241 cgtcgctttg ttgctcgctg atggcggaat atttttaata ttcagccaga agactgactc
    12301 gcgatcatca ggcaatgaca cacctttggt gtgaataatg cgtaatattt tttcttgcga
    12361 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
    12421 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
    12481 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
    12541 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
    12601 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
    12661 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
    12721 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
    12781 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
    12841 ttataaagtt taaccagcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
    12901 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
    12961 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
    13021 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
    13081 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
    13141 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
    13201 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
    13261 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
    13321 atattaataa gtgtattttt gattcaaagg aatagtacac caaagagacg gctttatcta
    13381 aaaataatat taattacgca aaaataaaat ataaatttat tgtttaaata ttatttaaac
    13441 agtaaaaaat caagtttgtt atcagacatt aatgataatt cagaatatat ttatatagaa
    13501 agatagatga atttattaat gtcagttata aaaaagggcc accggtcgg
//
LOCUS       DAQSWQ010000117        13260 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.118, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000117 DAQSWQ010000000
VERSION     DAQSWQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.118"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="R8G18_003480"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="R8G18_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HEE9797090.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="R8G18_003481"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="R8G18_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HEE9797091.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="R8G18_003482"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="R8G18_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HEE9797092.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="R8G18_003483"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="R8G18_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HEE9797093.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="R8G18_003484"
     CDS             complement(4036..5568)
                     /locus_tag="R8G18_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEE9797094.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="R8G18_003485"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="R8G18_003485"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEE9797095.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="R8G18_003486"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="R8G18_003486"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HEE9797096.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="R8G18_003487"
     CDS             complement(8663..10399)
                     /locus_tag="R8G18_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEE9797097.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="R8G18_003488"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="R8G18_003488"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HEE9797098.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="R8G18_003489"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="R8G18_003489"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HEE9797099.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="R8G18_003490"
     CDS             complement(12121..12855)
                     /locus_tag="R8G18_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HEE9797100.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="R8G18_003491"
     CDS             13056..>13260
                     /locus_tag="R8G18_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEE9797101.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3367 a         3372 c         3487 g         3034 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAQSWQ010000118        13210 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.119, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000118 DAQSWQ010000000
VERSION     DAQSWQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.119"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..807
                     /locus_tag="R8G18_003492"
     CDS             <1..807
                     /locus_tag="R8G18_003492"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEE9797102.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="R8G18_003493"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="R8G18_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HEE9797103.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="R8G18_003494"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="R8G18_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HEE9797104.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /locus_tag="R8G18_003495"
     CDS             complement(3460..6573)
                     /locus_tag="R8G18_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEE9797105.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="R8G18_003496"
     CDS             complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="R8G18_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HEE9797106.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="R8G18_003497"
     CDS             7815..8093
                     /locus_tag="R8G18_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797107.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="R8G18_003498"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="R8G18_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HEE9797108.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="R8G18_003499"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="R8G18_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HEE9797109.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10247..10579
                     /gene="hdeA"
                     /locus_tag="R8G18_003500"
     CDS             10247..10579
                     /gene="hdeA"
                     /locus_tag="R8G18_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HEE9797110.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10695..11021
                     /gene="hdeB"
                     /locus_tag="R8G18_003501"
     CDS             10695..11021
                     /gene="hdeB"
                     /locus_tag="R8G18_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HEE9797111.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11085..11732
                     /locus_tag="R8G18_003502"
     CDS             11085..11732
                     /locus_tag="R8G18_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEE9797112.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11774..12304)
                     /locus_tag="R8G18_003503"
     CDS             complement(11774..12304)
                     /locus_tag="R8G18_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9797113.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12460..13026)
                     /gene="slp"
                     /locus_tag="R8G18_003504"
     CDS             complement(12460..13026)
                     /gene="slp"
                     /locus_tag="R8G18_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HEE9797114.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3599 a         2983 c         3030 g         3598 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttc tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga
    10141 ttttgatatt ttatatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
    10201 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
    10261 aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca
    10321 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
    10381 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa
    10441 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
    10501 ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa
    10561 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac
    10621 aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt
    10681 gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt atgggcgctg
    10741 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
    10801 atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt
    10861 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
    10921 atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt
    10981 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
    11041 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
    11101 ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc
    11161 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
    11221 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactcgacc
    11281 ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc
    11341 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
    11401 ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
    11461 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
    11521 atttacagct gacattagtg aacggtaata tggtatcaat gctcgactgg tttaagcagc
    11581 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
    11641 ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta ttaaaaggta
    11701 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
    11761 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    11821 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    11881 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
    11941 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    12001 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
    12061 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
    12121 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    12181 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    12241 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    12301 acataaccga ctcctggtgc ggacattaac tttataatca atttgccaaa ggtaattaac
    12361 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
    12421 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    12481 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    12541 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    12601 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    12661 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactgggtc
    12721 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggttt
    12781 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    12841 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    12901 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
    12961 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
    13021 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
    13081 ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
    13141 ccttttgttt agttcaattc cattgatttt ggggcgtggc atgagtatcc atcgccccat
    13201 aatataatcc
//
LOCUS       DAQSWQ010000119        12996 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.120, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000119 DAQSWQ010000000
VERSION     DAQSWQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.120"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            96..1391
                     /locus_tag="R8G18_003505"
     CDS             96..1391
                     /locus_tag="R8G18_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEE9797115.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLVLAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="R8G18_003506"
     CDS             complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="R8G18_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HEE9797116.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2222..3325
                     /gene="asd"
                     /locus_tag="R8G18_003507"
     CDS             2222..3325
                     /gene="asd"
                     /locus_tag="R8G18_003507"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEE9797117.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3598..5784
                     /gene="glgB"
                     /locus_tag="R8G18_003508"
     CDS             3598..5784
                     /gene="glgB"
                     /locus_tag="R8G18_003508"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEE9797118.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5781..7754
                     /gene="glgX"
                     /locus_tag="R8G18_003509"
     CDS             5781..7754
                     /gene="glgX"
                     /locus_tag="R8G18_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEE9797119.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7772..9067
                     /gene="glgC"
                     /locus_tag="R8G18_003510"
     CDS             7772..9067
                     /gene="glgC"
                     /locus_tag="R8G18_003510"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEE9797120.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9067..10500
                     /gene="glgA"
                     /locus_tag="R8G18_003511"
     CDS             9067..10500
                     /gene="glgA"
                     /locus_tag="R8G18_003511"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEE9797121.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10519..12966
                     /gene="glgP"
                     /locus_tag="R8G18_003512"
     CDS             10519..12966
                     /gene="glgP"
                     /locus_tag="R8G18_003512"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HEE9797122.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3226 c         3575 g         3156 t
ORIGIN      
        1 ttccaccagt gtcaaaatct cgacggggcg tttgttatta cccctccttt gatgcctggt
       61 ccggcgttgc tggttgacga tatcgtggat tctgcatgga cgctgacagt tctgacagca
      121 ctgttacgcc aggcaggttg cccgacggtt tatcctcttg cccttgcttc tacctcggta
      181 aaaaattgat gaatctttca gccaatgcac aagcaactct cctgctaacc agcgattttt
      241 ctcgcgcggc ggcgagtgag tataaacctc ttagtaatag tgaatggggg aagtttgcat
      301 tatggctgaa gcaccaacgt atcagtcccg ccgagcttct ggtgccgcaa ccgcaagaga
      361 aacttacagg ctggagcgat ccgcgtattt ctcaggagcg tattcttggc ttgctggcgc
      421 gtggtcatag tctggtgttg gcggtagata agtggcaacg cgccggttta tggatcttaa
      481 cccgcggaga tgctgattat cccgttcgct tgaaaaaccg attgcgaacg gatgcacctc
      541 ccgttttatt tggctgcggg aataaagcat tactgcaagc ggaaggtatg gcgattgttg
      601 gctcgcgaga tgctccgact gacgatttgc gctataccca acaactggcc gcgaaactgg
      661 cccaacaggg gatttgcgtt atctctggtg gtgcgcgagg tattgatgaa tgtgcaatgg
      721 cgtcggcact ggaggccggg ggaactgccg ttggcgtatt agctgatagc ttgttaaaaa
      781 cgagtacgtt agtgaaatgg cgtgaagggc ttatagcagg caacctggtg ttgatttcgc
      841 cgttttaccc agaggtacgt ttcaccgtcg gcaatgcgat ggcgcgaaat aaatatattt
      901 attgccttgc tgaaagcgca atggttgtac gtgcgggaat gaccggtgga acgataaccg
      961 gggcgatgga ggcattaaaa catcagtggc tgcctgtgca ggttaaacca aatcaggata
     1021 tgcaatcagc caattcacga ttagtagaaa atggggcgtc atggagtgct gaacaggctg
     1081 agaatgtgac gatcagactg ccagacgttc ctgggctgat gtatgacaga gcactccgta
     1141 acgctcaacc agaactgttt tcgctgcatg aagatgacgc aaattacgca gtaatgcccg
     1201 cgtatacgcc tgtcgatttt tatcaactct ttgtggcgga actggcgatc cttgcaaagg
     1261 aatcgataag tattgaaagg ctggcgtctt gtactggttt aaccatcgaa caaattagtg
     1321 tgtggctgaa ccgcgcagaa gaagagggaa gggttatccg attgggcgaa ggtcattatc
     1381 agttcaggta ataaaacagg tgccgcgtct ggttgtgtgc agcacctgtt tgaaccttac
     1441 cccttcatcc acattcgaat gccgtcgagg aacatctggg ttgccatcat caccagaatc
     1501 aatcccatca ggcgttcaag tgcgttcacc cctttctcgc ccagcagacg taaaaatagc
     1561 gaagactgta gcaggatgac aaaggtgccg ccccaggcca gcagcagagc aatcaccaga
     1621 tgtcccatct gattcgggta ctgatgagac aacaacatca gcgtggcgag aatagtcggc
     1681 ccggcgacta acggaattgc caacggcacg ataaatggct cttcacctgc cggaagcccg
     1741 ctgctatttc ctgaagcgct ggggaaaatc atcttaatgg cgattagaaa cagaatgatg
     1801 ccgccagaaa tggagacggt ttctgctcgc aggctgagaa atgccagaat tttctcaccc
     1861 gcaaacagga acaccagcat taccaggaga gcaataagca actctcgcac catgattgcc
     1921 cgccgtcttt tcggttcagt atgtttcagt acggacatga aaataggcag gtttccgagc
     1981 ggatccataa tcaggatcaa taaaactgct gcagaaatga tttcattcat aactcaaatt
     2041 ccctgataat tgccgcggac tttctgcgtg ctaacaaagc aggataagtc gcattactga
     2101 tggcttcgct atcattgatt aatttcactt gcgactttgg ctgctttttg tatggtgaaa
     2161 gatgtgccaa gaggagaccg gcacatttat acagcacaca tctttgcagg aaaaaacgct
     2221 tatgaaaaat gttggtttta tcggctggcg cggtatggtc ggctccgttc tcatgcaacg
     2281 catggttgaa gagcgcgact tcgacgccat tcgccctgtc ttcttttcta cttctcagct
     2341 tggccaggct gcgccgtctt ttggcggaac cactggcaca cttcaggatg cctttgatct
     2401 ggaggcgcta aaggccctcg atatcattgt gacctgtcag ggcggcgatt ataccaacga
     2461 aatctatcca aagcttcgtg aaagcggatg gcaaggttac tggattgacg cagcatcgtc
     2521 tctgcgcatg aaagatgacg ccatcatcat tcttgacccc gtcaatcagg acgtcattac
     2581 cgacggatta aataatggca tcaggacttt tgttggcggt aactgtaccg taagcctgat
     2641 gttgatgtcg ttgggtggtt tattcgccaa tgatcttgtt gattgggtgt ccgttgcaac
     2701 ctaccaggcc gcttccggcg gtggtgcgcg acatatgcgt gagttattaa cccagatggg
     2761 ccatctgtat ggccatgtgg cagatgaact cgcgaccccg tcctctgcta ttctcgatat
     2821 cgaacgcaaa gtcacaacct taacccgtag cggtgagctg ccggtggata actttggcgt
     2881 gccgctggcg ggtagcctga ttccgtggat cgacaaacag ctcgataacg gtcagagccg
     2941 cgaagagtgg aaagggcagg cggaaaccaa caagatcctc aacacatctt ccgtaattcc
     3001 ggtagatggt ttatgtgtgc gtgtcggggc attgcgctgc cacagccagg cattcactat
     3061 taaattgaaa aaagatgtgt ctattccgac cgtcgaagaa ctgctggctg cgcacaatcc
     3121 gtgggcgaaa gtcgttccga acgatcggga aatcactatg cgtgagctaa ccccagctgc
     3181 cgttaccggc acgctgacca cgccggtagg ccgcctgcgt aagctgaata tgggaccaga
     3241 gttcctgtca gcctttaccg tgggcgacca gctgctgtgg ggggccgcgg agccgctgcg
     3301 tcggatgctt cgtcaactgg cgtaatcttt attcattaaa tctggggcgc gatgccgccc
     3361 ctgttagtgc gtaatacagg agtaagcgca gatgtttcat gatttaccgg gagttaaata
     3421 gagcattggc tattctttaa gggtggctga atacatgagt attcacagcc ttacctgaag
     3481 tgaggacgac gcagagagga tgcacagagt gctgcgccgt tcaggtcaaa aaaatgtcac
     3541 aaccagaagt caaaaatcca attggatggg gtgacacaat aaaacaggaa gacaagcatg
     3601 tccgatcgta tcgatagaga cgtgattaac gcgctaattg caggccattt tgcggatcct
     3661 ttttccgtac tgggaatgca taaaaccacc gcgggactgg aagtccgtgc ccttttaccc
     3721 gacgctaccg atgtgtgggt gattgaaccg aaaaccgggc gcaaactcgc aaaactggag
     3781 tgtctcgact cacggggatt ctttagcggc gtcattccgc gacgtaagaa ttttttccgc
     3841 tatcagttgg ctgttgtctg gcatggtcag caaaacctga ttgatgatcc ttaccgtttt
     3901 ggtccgctaa tccaggaaat ggatgcctgg ctattatctg aaggtactca cctgcgcccg
     3961 tatgaaacct taggcgcgca tgcagatact atggatggcg tcacaggtac gcgtttttct
     4021 gtctgggctc caaacgcccg tcgggtctcg gtggttgggc aattcaacta ctgggacggt
     4081 cgccgtcacc cgatgcgcct gcgtaaagag agcggcattt gggaactgtt tatccctggg
     4141 gcgcataacg gtcagctcta taaatacgag atgattgatg ccaatggcaa cttgcgtctg
     4201 aagtccgacc cttatgcctt cgaagcgcaa atgcgcccgg aaaccgcgtc tcttatttgc
     4261 gggctgccgg aaaaggttgt acagactgaa gagcgcaaaa aagcgaatca gtttgatgcg
     4321 ccaatctcta tttatgaagt tcacctgggc tcctggcgtc gccacaccga taacaatttc
     4381 tggttgagct accgcgagct ggccgatcaa ctggtgcctt atgctaaatg gatgggcttt
     4441 acccacctcg aactactgcc cattaacgag catcccttcg atggcagttg gggttatcag
     4501 ccaaccggcc tgtatgcacc aacccgccgt tttggtactc gcgacgactt ccgttatttc
     4561 attgatgccg cacacgcagc gggtctgaac gtgattctcg actgggtgcc aggccacttc
     4621 ccgaccgatg actttgcgct tgccgaattt gatggcacga acttgtatga acacagcgat
     4681 ccgcgcgaag gctatcatca ggactggaat acgctgatct acaactatgg tcgccgtgaa
     4741 gtcagtaact tcctcgtcgg taacgcgctt tactggattg aacgttttgg tattgatgcg
     4801 ctgcgcgtcg atgcggtggc gtcaatgatt tatcgcgact acagccgtaa agagggggag
     4861 tggatcccga acgaatttgg cgggcgcgag aatcttgaag cgattgaatt cttgcgtaat
     4921 accaaccgta ttcttggtga gcaggtttcc ggtgcggtga caatggctga ggagtctacc
     4981 gatttccctg gcgtttctcg tccgcaggat atgggcggtc tgggcttctg gtacaagtgg
     5041 aacctcggct ggatgcatga caccctggac tacatgaagc tcgacccggt ttatcgtcag
     5101 tatcatcacg ataaactcac cttcgggatt ctctacaact acactgaaaa cttcgtcctg
     5161 ccgttgtcgc atgatgaagt ggtccacggt aaaaaatcga ttctcgaccg catgccgggc
     5221 gacgcatggc agaaattcgc taacctgcgc gcctactatg gctggatgtg ggcattcccg
     5281 ggcaagaaac tgctgttcat ggggaacgaa tttgcccaag gccgtgagtg gaaccatgac
     5341 gccagcctcg actggcatct gttggaaggc ggcgataact ggcaccacgg tgtccagcgt
     5401 ctggtgcgcg atctgaacct cacctaccgc caccataaag caatgcatga actggatttt
     5461 gacccgtacg gctttgaatg gctggtggtg gatgacaaag aacgctcggt gctgatcttt
     5521 gtgcgtcgcg ataaagaggg taacgaaatt atcgttgcca gtaactttac gccggtgccg
     5581 cgtcatgatt atcgcttcgg cattaatcag ccaggcaaat ggcgtgaaat cctcaatacc
     5641 gattccatgc actatcacgg cagtaatgca ggcaatggcg gcacggtaca cagcgatgag
     5701 attgccagcc acggtcgtca gcattcacta agcctgacac taccaccgct ggccactatc
     5761 tggctggttc gggaggcaga atgacacgac tcgccattgg caaacccgct cccctcggcg
     5821 cgcattacga cggtcagggc gtcaacttca cacttttctc cgctcatgcc gagcgggtag
     5881 agctgtgtgt ctttgacgcc aatggccagg aacatcgcta tgacttgcca gggcacagtg
     5941 gcgacatttg gcacggttat ctgccggatg cgcgcccggg tttgcgttat ggttatcgcg
     6001 ttcatggccc ctggcaaccc gccgaggggc atcgctttaa cccggcgaag ttgttgattg
     6061 atccttgcgc gcggcaaatt gaaggggaat tcaaagataa cccgctgctg cacgccggtc
     6121 ataatgaacc tgactatcgc gacaacgccg ccattgcgcc gaaatgcgta gtggtggttg
     6181 atcactatga ctgggaagat gatgccccgc cgcgcacgcc gtggggcagc accatcattt
     6241 atgaagccca tgttaaagga ttaacgtacc tgcacccgga gatcccggtc gagatccgtg
     6301 gcacttataa agccctcggg catccggtga tgatcaacta tttgaaacag ttgggcatta
     6361 ccgcgctgga actgctgccg gtggcgcagt ttgccagtga accacgtctg caacgcatgg
     6421 ggctaagtaa ctactggggt tacaacccgg tggcgatgtt tgcgctgcat ccggcgtatg
     6481 cctgctcgcc agaaacggcg ctgcatgagt ttcgcgatgc aatcaaagca ctgcataaag
     6541 cgggtatcga agtcattctt gatatcgtgc tcaaccatag tgcggaactg gacctcgacg
     6601 gcccgttatt ctcgctgcgt gggatcgaca accgtagcta ttattggata agagaagacg
     6661 gcgattatca caactggacc ggttgtggca acacgctcaa tttgagtcat ccggcggtgg
     6721 tggattatgc ctgcgcctgc ctgcgttatt gggtagaaac ctgtcacgtc gatggtttcc
     6781 gctttgatct ggcggcagtc atgggccgta cgccagagtt ccgtcaggat gcgccgttgt
     6841 ttaccgctat ccagaactgc ccggtgctct cgcaggtgaa gttaattgct gaaccgtggg
     6901 atatcgccct tggtggttat caggtgggaa atttcccgcc gctgtttgcc gagtggaacg
     6961 atcatttccg cgatgctgcc cgtcgtttct ggctacatta tgatttgcct ctgggggcgt
     7021 ttgccgggcg ttttgctgcc tccagcgatg tttttaaacg taatggtcgt ctgccgagtg
     7081 ccgcgattaa tctcgtcact gcgcatgacg gttttacgct tcgcgactgc gtttgcttca
     7141 accataaaca caatgaagca aacggagaag aaaatcgcga cgggaccaac aacaattaca
     7201 gtaacaatca tggtaaagaa gggttaggcg gttctcttga cctggttgaa cggcggcgcg
     7261 acagtattca cgccctgtta acaacgttgt tgctctccca gggtacgccg atgttactgg
     7321 ccggtgacga acacggtcac agccagcatg gcaataacaa tgcctactgt caggataacc
     7381 aattaacctg gttggactgg tcgcaggcaa gcagtggttt aaccgcattt accgccgcgt
     7441 taatccatct gcgcaagcgc attcccgctt tggtggagaa tcgctggtgg gaagaaggcg
     7501 acggcaatgt ccgttggcta aatcgatatg ctcaaccttt aagcacggat gagtggcaaa
     7561 acgggccgaa acagctgcaa attctgctct cggatcgctt tttgatcgca attaacgcca
     7621 cgcttgaggt aacagagatt gttttacctg ctggggagtg gcacgccatt cccccattcg
     7681 ctggagagga taacccagtg attacggctg tctggcaggg acctgcacac ggattgtgtg
     7741 tgttccagag atgataaaaa aggagttagt catggttagt ttagagaaga acgatcactt
     7801 aatgttggcg cgccagctgc cattgaaatc tgttgccctg atactggcgg gaggacgtgg
     7861 tacccgcctg aaggatttaa ccaataagcg agcaaaaccg gccgtacact tcggcggtaa
     7921 gttccgcatt attgactttg cgctgtctaa ctgcatcaac tctgggatcc gtcgtatggg
     7981 cgtgatcacc cagtaccagt cccacactct ggtgcagcac attcagcgcg gctggtcatt
     8041 cttcaatgaa gaaatgaacg agtttgtcga tctgctgcca gcacagcaga gaatgaaagg
     8101 ggaaaactgg tatcgcggca ccgcagatgc ggtcacccaa aacctcgaca ttatccgccg
     8161 ttataaagcg gaatacgtgg tgatcctggc gggcgaccat atctacaagc aagactactc
     8221 gcgtatgctt atcgatcacg tcgaaaaagg cgcacgttgc accgttgctt gtatgccagt
     8281 accgattgaa gaagcctccg catttggcgt tatggcggtt gatgagaacg ataaaattat
     8341 cgaattcgtt gaaaaacctg ctaacccacc gtcaatgccg aacgatccga gcaaatctct
     8401 ggcgagtatg ggtatctacg tctttgacgc cgactatctg tatgaactgc tggaagaaga
     8461 cgatcgtgat gagaactcca gccacgactt tggcaaagat ttgattccca agatcaccga
     8521 agccggtctg gcctatgcgc acccgttccc gctctcttgc gtacaatccg acccggatgc
     8581 cgagccgtac tggcgcgatg tgggtacgct ggaagcttac tggaaagcga acctcgatct
     8641 ggcctctgtg gtgccggaac tggatatgta cgatcgcaat tggccaattc gcacctacaa
     8701 tgaatcatta ccgccagcga aattcgtgca ggatcgctcc ggtagccacg ggatgaccct
     8761 taactcactg gtatccggcg gctgtgtgat ctccggttcg gtggtggtgc agtccgttct
     8821 gttctcgcgc gttcgcgtga attcattctg caacattgat tccgccgtat tgttaccgga
     8881 agtatgggta ggtcgctcgt gccgtctgcg ccgctgcgtc atcgatcgtg cttgtgttat
     8941 tccggaaggc atggtgattg gtgaaaacgc agaggaagat gcacgtcgtt tctatcgttc
     9001 agaagaaggc atcgtgctgg taacgcgcga aatgctacgg aagttagggc ataaacagga
     9061 gcgataatgc aggttttaca tgtatgttca gagatgttcc cgctgcttaa aaccggcggt
     9121 ctggctgatg ttattggggc attacccgca gcacaaatcg cagacggcgt tgacgctcgc
     9181 gtactgttgc ctgcatttcc cgatattcgc cgtggcgtga ccgatgcgca ggtagtatcc
     9241 cgtcgtgata ccttcgccgg acatatcacg ctgttgttcg gtcattacaa cggggttggc
     9301 atatacctga ttgacgcgcc gcatctctat gatcgtccgg gaagcccgta tcacgatacc
     9361 aacttatttg cctataccga caacgtattg cgttttgcgc ttctggggtg ggttggggca
     9421 gaaatggcca gcgggcttga cccattctgg cgtcctgatg tggtgcatgc gcacgactgg
     9481 catgcaggcc ttgcgcctgc gtatctggcg gcgcgcgggc gtcctgcgaa gtcggtgttt
     9541 actgtgcaca acctggccta tcaaggcatg ttttatgcac atcacatgaa tgacatccaa
     9601 ttgccatggt cattctttaa tattcatggg ctggaattca acggacaaat ctctttcctg
     9661 aaggccggtc tgtactatgc cgatcacatt acggcggtca gtccaaccta cgctcgcgag
     9721 atcaccgaac cgcagtttgc ctacggtatg gaaggtctgt tgcaacagcg tcatcgtgaa
     9781 gggcgtcttt ccggcgtact gaacggcgtg gacgagaaaa tctggagtcc agagacggac
     9841 ttactgttgg cctcgcgcta cacccgcgat acgttggaag ataaagcgga aaataagcgc
     9901 cagttacaaa tcgcaatggg gcttaaggtt gacgataaag tgccgctttt tgcagtggtg
     9961 agccgtctga ccagccagaa aggtctcgac ctggtgctgg aagccttacc gggtcttctg
    10021 gagcagggcg ggcagctggc gctactcggc gcgggcgatc cggtgctgca ggaaggtttc
    10081 cttgcggcgg cagcggaata ccccggtcag gtgggcgttc agattggcta tcacgaagca
    10141 ttttcgcatc gcattatggg cggcgcggac gtcattctgg tgcccagccg ttttgaaccg
    10201 tgcggcttaa cgcaacttta tggattgaag tacggtacgc tgccgttagt gcggcgcacc
    10261 ggtgggcttg ctgatacggt ttctgactgt tctcttgaga accttgcaga tggcgtcgcc
    10321 agtgggtttg tctttgaaga taataatgcc tggtcgctgt tacgggctat tcggcgtgct
    10381 tttgtactgt ggtcccgtcc ttcactgtgg cggtttgtgc aacgtcaggc tatggcaatg
    10441 gattttagct ggcaggtcgc ggcgaagtcg taccgtgagc tttactatcg cttgaaatag
    10501 ttttcaggaa acgcctatat gaatgctccg tttacatatt catcgcccac gcttagcgta
    10561 gaagctctta agcactctat cgcttacaag ctgatgttta cgattggaaa ggacccggtc
    10621 gtcgccaata aacatgaatg gctgaacgca acgttatttg ctgtgcgcga tcgtctcgtg
    10681 gagcgctggt tacgttcaaa ccgtgcccag ttgtcgcaag aaacacgtca ggtttactac
    10741 ctgtcgatgg agtttttgat tggccgtacg ctctccaacg ccatgttgtc gctaggaatt
    10801 tacgaagatg tacagggcgc actggaagcg atggggttaa atctcgaaga gctgattgat
    10861 gaagaaaatg acccaggcct cggtaacggt ggcctgggac gtctggcggc ttgcttcctt
    10921 gattctctgg cgacgttagg gttgccgggg cgcggttacg gcatccgcta tgactacggt
    10981 atgttcaagc agaacatcgt taacggtagc cagaaagagt cgccagacta ctggctggaa
    11041 tacggtaacc cgtgggaatt caaacgccac aacacgcgct ataaagtccg ttttggcggt
    11101 cgcattcagc aggaaggtaa aaaaacgcgc tggattgaaa ccgaagagat tctgggagtc
    11161 gcttacgatc agataatccc tggttacgac accgacgcga ccaacacgct gcgtttgtgg
    11221 agtgcgcaag ccagtagcga aattaacctc ggtaaattca accagggtga ctacttcgcg
    11281 gcagtggaag ataaaaacca ctccgagaac gtatctcgcg tactgtatcc ggatgactcc
    11341 acctactccg ggcgtgagct gcgcctgcgt caggaatact tcctggtttc ctcgaccatt
    11401 caggacattt taagccgcca ttatcagttg cataaaacct acgataacct ggcggataaa
    11461 atcgcgattc atctcaatga tacccatccg gtactgtcga ttcctgagat gatgcgtctg
    11521 ctgatcgatg agcaccaatt tagctgggac gacgcgtttg aggtgtgttg tcaggtcttc
    11581 tcctacacta accacacgct gatgagcgag gcgctggaaa cctggccggt tgatatgctg
    11641 ggtaaaattc tgccgcgtca cctgcagatc atctttgaaa tcaacgacta tttcctgaaa
    11701 accttgcagg aacagtatcc gaacgatacc gatctgctgg gacgggcgtc gatcattgat
    11761 gaatccaacg gtcgtcgtgt gcgtatggcc tggctggcgg ttgttgtgag ccacaaagtt
    11821 aacggtgtat cggaactgca ctctaatctg atggtgcaat cgttgtttgc cgactttgcg
    11881 aaaatcttcc cgggtcgttt caccaacgtc accaacggtg tgacgccgcg tcgctggctg
    11941 gcggtagcga acccatcgct ttcagccgtg ctggacgaac acctgggccg taactggcgc
    12001 accgacctta gcctgcttaa tgagctgcaa caacactgtg atttcccaat ggttaatcac
    12061 gctgtgcatc aggcgaagct ggagaacaaa aagcgtctgg cagagtatat cgcccagcag
    12121 ctgaatgtgg tggtgaatcc aaaggcgttg ttcgatgtac aaatcaaacg tattcacgaa
    12181 tacaaacgtc aattgatgaa tgtgttgcat gtgattaccc gctataaccg catcaaggcc
    12241 gacccggatg cgaagtgggt accgcgcgtg aatatttttg gcggtaaggc ggcttcggcc
    12301 tattacatgg cgaagcacat tattcatttg atcaatgacg tagcgaaagt gatcaacaac
    12361 gatccgcaga ttggcgataa gctgaaagtc gtgttcatcc cgaactacag cgttagcctg
    12421 gcgcagttga tcattccggc ggcagatctg tctgaacaga tttcgctggc agggacggaa
    12481 gcttccggca ccagtaacat gaagtttgcg cttaacggtg cgctgactat cggtacgttg
    12541 gacggtgcga atgtcgagat gctggatcat gtcggtgctg acaatatctt tatttttggt
    12601 aacacagcgg aagaagtgga agaactgcgt cgtcagggct acaaaccgcg tgaatactac
    12661 gagaaagatg aggagctgca tcaggtgctg acgcaaatcg gcagcggtgt attcagtccg
    12721 gaagatccgg gtcgctatcg cgatctggtt gattcgctga tcaacttcgg cgatcactat
    12781 caggttctgg cggattatcg cagctatgtc gattgtcagg ataaagtcga cgaactctac
    12841 gagcttcagg aagagtggac cgcaaaagcg atgctgaaca ttgccaatat gggctacttc
    12901 tcttctgacc gtactatcaa agagtacgcc gatcatatct ggcatatcga tccggtgaga
    12961 ttgtaagttc accaataaat agaacggggc caaagg
//
LOCUS       DAQSWQ010000120        12563 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.121, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000120 DAQSWQ010000000
VERSION     DAQSWQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.121"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="R8G18_003513"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="R8G18_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEE9797123.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="R8G18_003514"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="R8G18_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HEE9797124.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="R8G18_003515"
     CDS             1797..1970
                     /locus_tag="R8G18_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797125.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="R8G18_003516"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="R8G18_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HEE9797126.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="R8G18_003517"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="R8G18_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HEE9797127.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="R8G18_003518"
     CDS             complement(4268..5191)
                     /locus_tag="R8G18_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9797128.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="R8G18_003519"
     CDS             5408..6709
                     /locus_tag="R8G18_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEE9797129.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="R8G18_003520"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="R8G18_003520"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HEE9797130.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="R8G18_003521"
     CDS             8771..8995
                     /locus_tag="R8G18_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEE9797131.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="R8G18_003522"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="R8G18_003522"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HEE9797132.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /locus_tag="R8G18_003523"
     CDS             complement(9589..10569)
                     /locus_tag="R8G18_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HEE9797133.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="R8G18_003524"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="R8G18_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEE9797134.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="R8G18_003525"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="R8G18_003525"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HEE9797135.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2927 c         3146 g         3282 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAQSWQ010000121        12071 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.122, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000121 DAQSWQ010000000
VERSION     DAQSWQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.122"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..953)
                     /gene="ydcO"
                     /locus_tag="R8G18_003526"
     CDS             complement(<1..953)
                     /gene="ydcO"
                     /locus_tag="R8G18_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HEE9797136.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="R8G18_003527"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="R8G18_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HEE9797137.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="R8G18_003528"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="R8G18_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HEE9797138.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="R8G18_003529"
     CDS             complement(3668..3898)
                     /locus_tag="R8G18_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HEE9797139.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="R8G18_003530"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="R8G18_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="R8G18_003531"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="R8G18_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HEE9797140.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6212
                     /locus_tag="R8G18_003532"
                     /pseudo
     CDS             4815..6212
                     /locus_tag="R8G18_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6457..7602
                     /locus_tag="R8G18_003533"
     CDS             6457..7602
                     /locus_tag="R8G18_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEE9797141.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7620..8633
                     /locus_tag="R8G18_003534"
     CDS             7620..8633
                     /locus_tag="R8G18_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEE9797142.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8634..9534
                     /locus_tag="R8G18_003535"
                     /pseudo
     CDS             8634..9534
                     /locus_tag="R8G18_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9524..10318
                     /locus_tag="R8G18_003536"
     CDS             9524..10318
                     /locus_tag="R8G18_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEE9797143.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10340..11764
                     /gene="patD"
                     /locus_tag="R8G18_003537"
     CDS             10340..11764
                     /gene="patD"
                     /locus_tag="R8G18_003537"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HEE9797144.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            11974..>12071
                     /locus_tag="R8G18_003538"
     CDS             11974..>12071
                     /locus_tag="R8G18_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HEE9797145.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         2946 a         2910 c         3220 g         2995 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gccagcttgc ggaacgtaaa caacgtgcct
     5941 ggcaggcact gctgcgttat ctgcctgcgg aagttaaaat tcatcatagt gacagcggtt
     6001 attttctctg gctggagctc cccgagccgt tagatgccgg cgaattaagc ctggcggcac
     6061 tgacgcatca tatcagtatt gcgccgggta aaatgttttc taccggtgaa aactggtcac
     6121 gtttttttcg ttttaatacc gcgtggcagt ggggagaacg tgaagaacag gcggtaaaac
     6181 aattaggcaa acttattcaa gaacggctgt aatagcgttt aatttaattc ctcttagatt
     6241 gggtaatatg aatttcgaat agcagtcata tttcctaacc ccttgtctat actccagaag
     6301 ataaccttac agacggcata atgcgcggta gctcacaacc tgattaaatt ttctcaggga
     6361 cgaaggtgtg cctgcaagcc gccgtctatg gttaaacaag gagatatttt tacggcacgg
     6421 cggctgaaca attaattacg acaggagtaa gaccttatga gcaagacatt tgcccgcagc
     6481 agcctgtgtg cgctcaccat gacaataatg accgctcacg ccgccgaacc acctaccaat
     6541 ttagataaac cggaagggcg actggatatt atcgcctggc cgggatacat cgaacgcgga
     6601 caaactgata aacaatacga ctgggtaacg caatttgaaa aagagacagg ctgcgcggtg
     6661 aatgtgaaaa ccgccgcgac ttccgatgaa atggtcagtc tgatgaccaa agggggttac
     6721 gatctggtta cggcatccgg cgatgcctcg ctgcgtttga ttatgggcaa acgcgtgcag
     6781 ccgattaata ccgccttgat tcccaactgg aaaacgctcg atccgcgcgt ggttaaaggc
     6841 gactggttta atgttggcgg caaagtttac ggcacacctt accaatgggg gccgaacctg
     6901 ctgatgtaca acactaaaac cttcccgacg ccgccggata gctggcaagt ggtttttgtt
     6961 gagcaaaatc tgccggacgg caagagcaat aaaggccgcg ttcaggctta tgatggccct
     7021 atctacattg cggacgctgc gttgttcgtt aaagccactc agccgcagtt gggcatcagc
     7081 gatccgtatc aactaaccga agaacagtac caggcggtgc tgaaagtgct gcgcgctcaa
     7141 cacagtttga tccatcgcta ctggcatgac actaccgtgc aaatgagcga tttcaaaaac
     7201 gagggtgtgg ttgcttccag tgcctggccc tatcaggcca acgccctgaa agccgaaggc
     7261 cagcctgttg ctaccgtttt cccgaaggag ggtgttaccg gttgggctga taccaccatg
     7321 ctgcatagcg aagcgaaaca tccggtttgc gcctacaaat ggatgaactg gtcattaacg
     7381 ccaaaagtgc agggcgatgt ggcggcctgg tttggctcgt taccggtagt gccggaaggg
     7441 tgtaaagcca gtccgttatt aggcgaaaaa ggttgtgaaa ccaacggttt taactatttc
     7501 gacaaaatcg ccttctggaa aacgcctata gcagaagggg gcaagtttgt tccctacagt
     7561 cgctggacgc aggattacat tgccattatg ggcggtcgct aacttcgctg gggtgcttta
     7621 tgacgtacgc agtggagttt gacaacgtct cgcggttgta cggtgacgtg cgcgcagtag
     7681 atggcgtcag tattgcgata aaagatggtg agttcttctc tatgctgggg ccgtccggct
     7741 ccggcaaaac cacctgcctg cgcctgattg ctggcttcga acagctttcc ggcggggcta
     7801 tctctatctt tggtaaaccc gccagcaatc tgccaccgtg ggagcgggac gtgaatactg
     7861 tctttcagga ctacgcgcta tttccgcata tgtcgattct tgacaatgtc gcctatgggc
     7921 tgatggtcaa aggcgtgaat aaaaagcagc ggcacgcaat ggcgcaagag gcgctggaga
     7981 aagtggcgtt ggggtttgta cagcaacgta aaccgtcaca actttctggt ggtcagcgcc
     8041 agcgggttgc tatcgccaga gcattggtga atgaaccgcg cgtattgctg ttggatgaac
     8101 cgctcggcgc actggatctc aaattgcgtg agcagatgca gctggaactg aaaaaactgc
     8161 aacagtctct cggtatcact tttatcttcg ttacccacga tcagggcgaa gcgttatcga
     8221 tgtccgatcg tgtggcggtt ttcaataatg gacgcattga gcaggtcgat tccccgcgcg
     8281 atctctatat gcgcccgcgc acgccgtttg ttgccgggtt cgttggtaca tcgaatgttt
     8341 ttgatggact gatggcagag aaactttgtg gcatgacggg aagcttcgcc ctgcgaccgg
     8401 aacatatccg cctcaacacc cctggtgaac tgcaggccaa tggcacgatc caggcggtgc
     8461 aatatcaggg cgcggcaact cgttttgaac tgaaattgaa cggcggtgaa aaactgcttg
     8521 tgagtcaggc caatatgaca ggcgaagaac tgcctgccac gctcacgccc ggacaacagg
     8581 tgatggtttc ctggtcgcgt gatgtgatgg tgccgctggt tgaggagagg tgaatggcga
     8641 tgaatgtatt gcaatcacct tcacgtccag gtctggggct gtttttactg ctgcttggcc
     8701 cgctaatgtg gtttggcatt gtctatttcg gctcgctgct gacactgtta tggcagggat
     8761 tttatacttt tgacgatttc accatgtcgg taacgccgga actgacgctg gcgaatatcc
     8821 gtgcgctgtt taatccggcg aattacgaca tcattctccg cacgctgact atggctgtgg
     8881 cagtcactat cgccagcgcc attctggctt ttccaatggc gtggtatatg gcgcgctata
     8941 ccagcgggaa aatgaaagcg tttttatatt gcggtaatgt tgccgatgtg ggcgagctac
     9001 attgttaaag cctatgcctg gacgttattg ctggcaaaag atggcgtggc tcagtggttt
     9061 ttacaacatc ttgggctgga accactgctg actgcgttcc ttacattacc tgcggtgggc
     9121 ggaaatacgc tgtcaacttc cgggctgggg cgctttctgg tgtttctcta tatctggttg
     9181 ccgttcatga tcctgcccgt tcaggcggcg cttgagcgtt tgccgccgtc attgttgcag
     9241 gcgtcggctg atctcggcgc acgtccacga caaacctttc gctatgtggt gctgccgctg
     9301 gcaatcccgg gtattgccgc tggctctatc tttaccttct cactcacact gggcgatttt
     9361 atcgtcccgc agctggttgg tcctccagga tattttatcg gcaatatggt ttattcccag
     9421 cagggggcga ttggcaatat gccgatggcg gcggcattca ccctggtgcc gattattctc
     9481 atcgcactgt acctggcgtt cgtgaaacgt ctgggagcgt tcgatgcact ctgaacgcgc
     9541 accgtttttc ctcaaactgg cggcctgggg cggcgttgtt ttcctacatt ttcccatcct
     9601 gataatcgcc gcctatgcgt ttaacactga agatgcggcg tttagttttc caccgcaggg
     9661 cctgacgctg cgctggttta gcgtggcagc acagcgtagt gatattcttg atgccgtgac
     9721 actgtcactt aaagtggcgg cgctggcgac attaattgcg ctggtgttag ggacgctggc
     9781 agctgccgcg ctgtggcgac gagacttttt cggcaaaaac gccatttcgc tgttactgct
     9841 gctgcccatt gcgctgccgg gcagcgtcac tggtctggcg ttattaaccg cctttaaaac
     9901 catcaatctg gagccgggat ttttcaccat cgtggtcggt catgcgactt tttgtgtagt
     9961 tgtggtgttt aacaatgtca tcgcccgttt tcgccgcacc tcctggagtc tggttgaggc
    10021 gtcaatggat cttggggcca atggctggca aaccttccgc tacgtagtgt tgccgaatct
    10081 cagttcggcg ttactggcag gaggaatgct ggcgtttgcc ttgtcgttcg atgaaatcat
    10141 cgttacgacc tttacggcag gtcatgaacg aacgttactg ttgtggttgc tcaatcagct
    10201 tgggcgaccg cgtgatgtac cggtaactaa cgtggtggca ctgctggtta tgttggtaac
    10261 aaccttgccg atcctggggg cctggtggct aacccgcgaa ggcgacaatg gtcaataacc
    10321 actgatacag gaatatgcta tgcaacataa gttactgatt aacggagaac tggttagcgg
    10381 cgaaggggaa aaacagcctg tctataatcc ggcaacgggg gacgttttac tggaaattgc
    10441 cgaggcatcc gcagagcagg tcgatgctgc tgtgcgcgcg gcagatgcag catttgccga
    10501 atgggggcaa accacgccga aagtgcgtgc ggaatgtctg ctgaaactgg ctgatgttat
    10561 cgaagaaaat ggtcaggttt ttgccgaact ggagtcccgt aattgtggca aaccgctgca
    10621 tagtgcgttc aatgatgaaa tcccggcgat tgtcgatgtt tttcgctttt tcgcgggtgc
    10681 ggcgcgctgt ctgaatggtc tggcggcagg tgaatatctt gaaggtcata cttcgatgat
    10741 ccgtcgcgat ccgttggggg tcgtggcttc tatcgcaccg tggaattatc cgctgatgat
    10801 ggccgcgtgg aaacttgctc cggcgctggc ggcagggaac tgcgtagtgc ttaaaccatc
    10861 agaaattacc ccgctgaccg cgttgaagtt ggcagagctg gcgaaagata tcttcccggc
    10921 aggcgtgatt aacatactgt ttggcagagg caaaacggtg ggtgatccgc tgaccggtca
    10981 tcccaaagtg cggatggtgt cgctgacggg ctctatcgcc accggcgagc acatcatcag
    11041 ccataccgcg tcgtccatta agcgtactca tatggaactt ggtggcaaag cgccagtgat
    11101 tgtttttgat gatgcggata ttgaagcagt ggtcgaaggt gtacgtacat ttggctatta
    11161 caatgctgga caggattgta ctgcggcttg tcggatctac gcgcaaaaag gcatttacga
    11221 tacgctggtg gaaaaactgg gtgctgcggt ggcaacgtta aaatctggtg cgccggatga
    11281 cgagtctacg gagcttggac ctttaagctc gctggcgcat ctcgaacgcg tcagcaaggc
    11341 agtagaagag gcgaaagcga cagggcacat caaagtgatc actggcggtg aaaagcgcaa
    11401 gggtaatggc tattactatg cgccgacgct gctggctggc gcattacagg acgatgccat
    11461 cgtgcaaaaa gaggtatttg gtccagtagt gagtgttacg cccttcgaca acgaagaaca
    11521 ggtggtgaac tgggcgaatg acagccagta cggacttgca tcttcggtat ggacgaaaga
    11581 tgtgggcagg gcgcatcgcg tcagcgcacg gctgcaatat ggttgtacct gggtcaatac
    11641 ccatttcatg ctggtaagtg aaatgccgca cggtgggcag aaactttctg gttacggcaa
    11701 ggatatgtca ctttatgggc tggaggatta caccgtcgtc cgccacgtca tggttaaaca
    11761 ttaagttaga tgttagtggc tgttatagga cggccactat cgttttaggt tctgagttta
    11821 atggcggtta agtattagaa tgattacatt aataactagc gtaaaaatta ggctaaatgt
    11881 ttgatggcga tttttcatcc tgtcacagtt gattagcata aggctttaga gtatcgttat
    11941 taaactaata atttataagt agaagtatta acaatgaaag ttcaattgta caaattcacg
    12001 gaagataaaa ataaaaccct taccttcaga tggacgaaaa aacactttga gttttgtatg
    12061 gataataaaa t
//
LOCUS       DAQSWQ010000122        12056 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.123, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000122 DAQSWQ010000000
VERSION     DAQSWQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.123"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            43..303
                     /locus_tag="R8G18_003539"
     CDS             43..303
                     /locus_tag="R8G18_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797146.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            300..890
                     /locus_tag="R8G18_003540"
     CDS             300..890
                     /locus_tag="R8G18_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797147.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(910..1257)
                     /locus_tag="R8G18_003541"
     CDS             complement(910..1257)
                     /locus_tag="R8G18_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797148.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            3239..3925
                     /locus_tag="R8G18_003542"
                     /pseudo
     CDS             3239..3925
                     /locus_tag="R8G18_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            4258..5133
                     /locus_tag="R8G18_003543"
     CDS             4258..5133
                     /locus_tag="R8G18_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEE9797149.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            5141..5917
                     /locus_tag="R8G18_003544"
     CDS             5141..5917
                     /locus_tag="R8G18_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEE9797150.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            6086..8347
                     /locus_tag="R8G18_003545"
     CDS             6086..8347
                     /locus_tag="R8G18_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEE9797151.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            8416..9591
                     /gene="senB"
                     /locus_tag="R8G18_003546"
     CDS             8416..9591
                     /gene="senB"
                     /locus_tag="R8G18_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HEE9797152.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(10117..10578)
                     /locus_tag="R8G18_003547"
                     /pseudo
     CDS             complement(10117..10578)
                     /locus_tag="R8G18_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(10616..>12056)
                     /locus_tag="R8G18_003548"
     CDS             complement(10616..>12056)
                     /locus_tag="R8G18_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEE9797153.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYA
                     DDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESY
                     LRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT         3215 a         2846 c         2905 g         3090 t
ORIGIN      
        1 tgatttttac ctgtcagaac attatcagat ggaacgtgca ggatggatca ggaaatgacg
       61 ttttcactca gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat
      121 ctggacagcc acggcgtaac ttatctctgc gagtcagcca aagccggagc cgtgctgatc
      181 ttctggcatg aactggccat aaatggctat acctcaatga atgccataaa gagacaggag
      241 atcattgacg ccgaccatca gcgcctcaga aagctcatct ggccggagga tgactggaaa
      301 tgactcagag ccgccgaccg tcgccactgc agcggagagt gctgattgtg ctggctgccc
      361 ttgatgaaaa acggccagga cccgtgctga cgcgggacat agaacgggtg ctggagcaaa
      421 gcggggaagc cccggtctac gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg
      481 ccggctggct gcgtaccctg cgcgctccga acctgcagct ggcagtggag ctgacggacg
      541 ccggccgggc cgtggcacag cctctgctgc tggcagaaca ggaccgcatc cgggcagaac
      601 agcgtgcggc agaagtcgtc gtactgccgc tggtcccggc agcaggtctg cccgcaggcg
      661 gcacttctgc cactgatctg gcggtggagc tgaacggcat aacgtatcag gcctgccgtg
      721 gggattttgt ggtgcgtctc gacggcagca cctgtctgca gttatggaat aaagaaggct
      781 gggtggtacg ccgggagggc gatccgctgg aagtggcgca gtggctgcag gcctgtcacg
      841 atgcaggaat ggaggttcgc gtacagataa atgaaagtgc cgctccgtaa gaacacgcct
      901 gtgcacggtt tacacatcat cccatggtgg cagtcggtta actttcctgc gccaccagtg
      961 gaaagaggcc atgagcagtc cggacaaaaa accccccgtg atacttataa taatggcctc
     1021 acctgccacc attcctgacg gcccccagta gataaaccac attgcacatc cccaggaaat
     1081 gccccataag ccccccgtca gcaccgtgac ctgccagaac ggcataaagg gtaatggcgg
     1141 aagccggata cccagccgcc agagaatacg cagaagagga ggggcataat tgctccgcca
     1201 catctttttg ctgtccatca gggcaatggc ccgggctttt ttctgctcaa aagtcacagg
     1261 agcacctccg ttccatgatg taaacgtgac acagcttatt gttattgatt attattatca
     1321 tgctgtggtt atgttgtcat gcatcagtac actgacaaaa gtgcaaaaga aaggaaaact
     1381 ggcagatgaa cagaaaatat tattttaata atatgtggtg gggatgggtg acgggaggat
     1441 atatgctgta tatgtcatgg gattatgatt ttaaatacag attactgttc tggtgtattt
     1501 ctctctgcgg aatggttttg tatccggttg caaaatggta tattgaagat acagctctga
     1561 aatttacccg gcctgatttc tagaacagcg gtttttttgc tgatacacct ggaaaaatgg
     1621 gattgcttgc tgtttatacg ggtactgttt tcatattatc tcttccgtta agtatgatat
     1681 atattctttc tgttattata aaaaggctgt ctgtaagata gacagcctgt cttatatttt
     1741 atttaaatac cccagaactt atgccacaag aattatctaa aagttgccgt tctactccgt
     1801 tagtttgtag ttctatcata gtcttattaa ctcgatcacg aggtctgtat gacggggaag
     1861 cttgcgctgt aagattatat gataatgcac atattgtaat gatagttgtt tttatctttg
     1921 tgtttaatgt gaaaatttta taaagcatat aatgttccct atttttattt tgtttataat
     1981 ttattggttg tatgttaaat ctatcataag tctaatgatt aaaatggtta gtggtgtcag
     2041 gattagtgaa aataaaggat gtaatatgct attctttctc tgtaagtact tgagaatact
     2101 aaaccatagt acaagaacga ttatgtacat gtagtgagaa ttggaaaaca tagtttcttc
     2161 ccgtgttaat gagagtatgt gtttttatgt ggggatatgt acaccccata tagatgtatt
     2221 atgttatatt tataatatac ctcggcaaaa taaaaggatt gttatagaaa tagggaagct
     2281 ataaaaaagc ttatattaag gtctgttttc ttcttagaaa aataattctt tgttctaatt
     2341 attatagatg taatccccca aacaataaaa agaatggagc tgcttgtaat tatttgatat
     2401 atcaaaattg ttttcctcta tttttgtgta gatagatgtt aatggcactg ggtgcaatca
     2461 attaagaaaa ttgacgccag actaattatt aataatccag ttatcttttt attcatatca
     2521 tctcctttcc attggttaat ttatcggaaa ttcatcccca ctaactaaaa cgatccacac
     2581 aataaaacta aatctaattg caggcaatca attccagtcg atatatcatc tgtatggaaa
     2641 tcatcgctca tcactcatcg cagcctgaaa gcccagagca acggggcagg tctgcatata
     2701 agtggttaca cggcagaatt ccagagggtt ctgtcactaa attcagtcac tgacaaattt
     2761 gcatgatgtt gaatagacga gattttgtgt cgaaatagtt gtttcagtaa ctgtcactca
     2821 atcggtggtt tatgtgacta tattcgcaaa aactggtcgc agaaaagtcc tgttttcagc
     2881 ttggaaaccc ggtaatcaga ataatggata ggggaacaaa aatctctgta tagaacgctc
     2941 cattgaggga gagaaaacgt gtcattttga cccgttcggt attcagaaca cacaaaaccg
     3001 atcgcgggtc gctttcgtgc atagttatgc aacgcgctaa aaacgattct gacgcatttt
     3061 ttatgtttac cctgtttttt cttgtctttt tgagtttttt gcgtcagaga gcgtctgaga
     3121 gggcacgtac aacgggagtt atggtaaatg gatcgggttt tcgggaaggt tcgacaggat
     3181 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatc atgtccagat
     3241 ccggcagaac cagcctcatg cgcagttctg gctggttttt gacgttgatc gcgagggggc
     3301 agcgattgac tggagcgacc ggaacgctcc cgcgccgaac atcaccgtaa aaaatcctgt
     3361 gaacggtcat gctcacctgc tctacgcgct caatatcgcc gtgagaaccg cgccggacag
     3421 ctcggttaag gcgctgaaat acgccgctgc gattgaacgt tcgctgtgtg agaaactttg
     3481 tgctgatgtg aactacagcg gactgatttg taaaaatccg ttccacctgg aatggcaggt
     3541 gatggagtgg cgagaggaag cctataccct cgatgaactg gctgattatc tcgatttgag
     3601 cgcctcagcg cgccgtagca tcgataaaca ttacgggatg gggcgaaact gccacctgtt
     3661 cgaaatgacg cgcaaatggg cttacagggc gattcgtcag ggctggcccg aattctcaca
     3721 gtggcttgat gccgtgattc agcgtgtcga aatgtacaac gcatcgcttc ccgttccgct
     3781 ttcaccgcct gaatgtcggg ctattggcaa gagcattgcg aaatacacgc acaggaactt
     3841 cacgccggaa actttcgcac agtatgtggc tgatacgcac acattaacgt acgttttcgt
     3901 tccattggcc ctcacccccc gataacgacc caggtagcgc gccgtattcc ccccgccggc
     3961 cgttttcttc gacatgtaca tcaggcgcgg aactcatcgg ggcgcgggaa tgaaatccca
     4021 ttcagactcc ggatagattc aagcaagccc acttatcgtc aaaatcggtg ttgcaaaaac
     4081 gggagtgacc atagatccgt tttccaagcg gaccccataa tatcggctgg taaacatcac
     4141 tcgctagtgc attctttttg ttggtaatga taatcattta cattcattct ttgatgtttt
     4201 atcataatta atgttttgtc aataaatata tggaaacata ttgtttcaga tgggaatatg
     4261 agaaagttca tccttatttc tatgataacg ttagctagtg ttagtctggg agcctgcaga
     4321 cagaatgtca ccataaagca ggatgcgcca gggcagaaag ctttcctgac tgacgggcag
     4381 atttacgatt tgcacagtgg caaaattatt tctagcagcg agcttttggc ggatcttgct
     4441 accgctcgac atcttattat tggtgaaaaa cacgataatg ccgagcatca tcagattgag
     4501 ctttggctta ttcaaaattt gctcatccag cgaccacagg ggagtgtgtt gcttgagatg
     4561 ttgacgtcag agcaacagcc ccgagttaac caagttaaat gttggctgaa agataatcct
     4621 gtggttcgag atagccgtgt tcaggaacta cttaactggc aaaaagggtg gtcctgggag
     4681 atgtatggcg acatcgtcat gcagctatta cgtgggcctt acccgctgct caatgccaac
     4741 attggccggg agcagatgtt ggcactgtac aaaaaaaacg aatttcctaa aggaaaaaaa
     4801 tccactgcac ctgtggtaca agaagctctg cgtgaaacca tcatatctat gcatgaaggc
     4861 aaccttgaaa gccagcaact gacgtcaatg ctctctattc agcagcagcg ggatcgctac
     4921 atggcccgcc agcttctgag tgccccggta ccgtcgttgt tgattgcagg tggttatcac
     4981 gcgtcaaaaa gcatgggcgt accgttacac atggaagatc ttgccaccgg cacccatcca
     5041 gtggtgttga tgcttgcaga aaaaggaatg aacatcacag tagatcatgc tgactacgtg
     5101 tggtttgtag cgccagatac aacaaaaagg taaaccatgg atgatgaata ttctccactt
     5161 ctcacagtcg gtaaaatgga gcagctggtt tatctgttct ctactgcttc atggactgat
     5221 tttccttgcg tttatctggc gtttttccga agttcagccc gctatgtctc ctgcaccggc
     5281 gatcatgctg caatgggcag aaattataga ggctccttct tctccacttt cgcttccggt
     5341 gggaatcgct caacaggagt ccgcagtgac agaagaaaag caacaaactg aggacagaca
     5401 acaacgtcct gtcactgaag acagtgatgc aaccattgaa ataactagga agaaaaagtc
     5461 atccgatgga gaaaagaaaa agacacgtcc acctcgtaag ataaaggctc aaaccagcga
     5521 cagcaacccc acagccgttt ccagtaatgc tgcacctcag gctctggtcg aatcgtcccg
     5581 tattgctgcg ccttttaaca gtgactcaac aaagcgagat aattctgagg catcttggga
     5641 aagcagagta aaaggccacc taaaccgtta taaacgttac cctggcgatg ccagaaaacg
     5701 tgcacgaacc ggcacagccg tcgtgacttt taccgtaaac acggaaggca ctattgtcag
     5761 tagtttcttg gaaatctctt caggaaccct ttctcttgac cgcgaagcta ttgctgtttt
     5821 agaacgcgct caaccacttc ccaaaccacc gcctgaaata ttggagggag gattatttaa
     5881 agtaaaaatg ccgattacct ttaagttgaa ggagtaaagg gataattggc cgttcaagtt
     5941 catttaactg aaatttaatt ttcattttta tgctgaacat tttatcaaac ttttccactg
     6001 tcgaaaaaat caaaaagaca gtagaacata attaataaac aaatagttat acttatcttt
     6061 attaatatta tacccaagga aatttatgaa cgttataaaa ctggctatcg gctcaggcat
     6121 attattgctc agctgcggtg cttactcaca atccatcagt gaaaaaacta attccgacaa
     6181 aaaaggagcg gcagaattca gtccgctcag cgtttctgtc gggaagacga ccagtgagca
     6241 ggaagctctc gagaaaacag gcgcgaccag ttcccggaca acggacaaaa acctgcaatc
     6301 acttgacgca acagtgcgta gtatgcctgg tacttatact caaatagatc ctggtcaggg
     6361 agcaatcagt gtgaatattc gaggcatgag cggatttggt cgtgtaaaca ctatggtcga
     6421 tggtattacc cagagttttt acggaacctc tacctccgga acaacgacgc atggttcaac
     6481 taacaatatg gctggcgtac ttatagatcc taacttactg gtagcagttg atgttacacg
     6541 cggtgacagc agtggctctg aagggatcaa cgcccttgcc ggtagtgcaa atatgcgtac
     6601 tattggcgtt gacgatgtaa tatttaacgg taatacatat ggccttcgtt cacgtttctc
     6661 tgtcggtagt aatgggctgg gacgcagcgg aatgatcgcc cttggtggaa aaagcgacgc
     6721 ttttacggat acgggaagca ttggcgttat ggctgctgtg agcggcagtt ctgtgtactc
     6781 taatttctca aatggttctg gaattaacag caaagagttt ggttatgata aatatatgaa
     6841 gcagaacccc aaatcccaac tgtataaaat ggatatcaga ccagacgaat ttaacagctt
     6901 cgaactttcc gctcgaacct atgaaaataa atttacacgt cgtgatataa ccagtgacga
     6961 ctattacatt aaatatcatt acaccccttt ttctgaatta attgacttta acgtaacggc
     7021 cagtaccagt cgcggtaatc aaaagtatcg tgatggctcg ctgtatactt tctacaaaac
     7081 ctcagcgcaa aatcgttctg acgcgctgga tatcaacaat accagccggt tcactgtcgc
     7141 ggacaatgac ctggagttta tgctgggcag caaactgatg cgtacccgct atgaccggac
     7201 cattcactca gcggcgggcg acccgaaagc gaatcaggaa tcgatcgaga acaatccgtt
     7261 cgcaccctcc ggccagcagg atatttcagc gctgtatacc gggctgaagg ttacgcgcgg
     7321 catctgggag gcagatttca atctcaacta cacacgtaac aggatcacag ggtacaagcc
     7381 cgcctgcgat tcacgcgtta tctgcgtgcc acagggtagc tacgatattg acgataaaga
     7441 gggtggcttc aacccttcag ttcagctttc tgctcaggta acaccatggc ttcagccgtt
     7501 cattggctac agcaaatcca tgcgcgcccc gaacatccag gagatgttct tctctaattc
     7561 aggaggcgca tccatgaacc cattcctgaa gcctgaacgt gcagaaacct ggcaggcggg
     7621 ttttaacatt gataccagag atttactggt cgaacaggat gccctgcgct ttaaggctct
     7681 ggcgtaccgc agcaggatcc agaactacat ctacagcgag tcttatctgg tttgttctgg
     7741 aggtcgtaaa tgcagtatgc ctgaggtgat tggcaatgac tgggagggca ttagcgatga
     7801 atacagcgac aatatgtaca tctacgttaa ctcggcaagc gacgttatcg caaagggctt
     7861 cgaactggag atggattatg atgcaggttt tgcttttggc cgactctctt tcagccagca
     7921 gcaaacagac cagccaacct ccatcgccag cacctacttt ggcgcagggg atatgaccga
     7981 actgcccaga aaatacatga cgctggatac tggtgttcgc ttcttcgata acgcgttgac
     8041 cctgggcact atcataaaat acacaggcaa ggctcgtcgc ctgtcgcctg attttgagca
     8101 ggacgaacat accggcgcaa taatcaaaca ggatttgccg cagatcccaa cgattatcga
     8161 tctctatggt acttacgagt acaaccgcaa cctaacactg aaactttcgg tacaaaacct
     8221 gatgaacaga gattattcgg aggcgctgaa taagctcaac atgatgccag gtcttggtga
     8281 cgagacccac ccagccaatt ccgcgcgtgg cagaacatgg atatttggcg gggacattcg
     8341 tttctgatga caatttccgc taggttcaga caatacgttt tctctcttat gtcaatttta
     8401 ttgcaggaac gaaaaatgaa tattttcact ttatccaaag caccgctata cttgttaatt
     8461 tcactatttt tacccacgat ggccatggct atcgatccac ctgaacgcga actttcgcga
     8521 tttgccctga aaacgaatta ccttcagtcc cctgatgaag gcgtctatga actggcgttt
     8581 gataatgcca gtaaaaaggt gtttgcagca gtcaccgatc gtgtaaatcg tgaagccaat
     8641 aaaggctatc tgtattcgtt taattcagat tcgctgaaag tcgaaaataa atacacgatg
     8701 ccataccggg cattttcgct ggcgataaat caggataaac atcagctcta tatcggacac
     8761 acccagtcag cgtccctgcg tatcagtatg tttgacaccc caaccggcaa actggtaaga
     8821 accagcgaca ggttaagttt taaagcggca aacgctgcag attcgcgttt tgagcatttt
     8881 cgccatatgg tttacagcca ggattccgat accctgtttg tgagttatag caatatgctg
     8941 aaaacggccg agggcatgaa gcctctgcat aagctgttaa tgctcgacgg gacgacgctt
     9001 gccttaaaag gcgaggttaa ggatgcttac aaaggtacag cgtatggtct gacgatggat
     9061 gaaaaaacac agaaaatcta cgttggcgga agagattaca tcaacgaaat tgatgcgaaa
     9121 aatcagacgc tgctgcgtac catcccgttg aaagatccga gaccacaaat cacaagtgtg
     9181 cagaatctgg cggtggactc cgcttctgac cgtgcctttg tggtggtatt cgaccatgac
     9241 gatcgttccg gtacaaaaga tggactctat atttttgact tacgcgacgg taaacagctt
     9301 ggctatgtgc acacaggagc cggagctaac gcggtgaaat acaatccgaa atataacgaa
     9361 ctgtatgtca ccaacttcac tagcggcacc atcagcgtag tggatgccac caaatacagc
     9421 atcacccgtg aatttaacat gccggtctac ccaaaccaga tggtgttgtc ggacgatatg
     9481 gatacccttt acattggcat caaagaaggc tttaaccgcg attgggatcc tgatgtgttt
     9541 gtggaaggag ctaaagaacg tattctgagc attgatttga aaaagtcgtg atcgggggga
     9601 aaaaacatgg gataaaaact ccgttattcc actaaactgg ccggactagg aaaagcaaac
     9661 atctctgtcc tgccggtacg cctgttacgc cgctccatga ggctgaggaa tctccagaaa
     9721 acgagacagg cacagcattg tacgatcaac tgatttgtgc caataattaa atgaaatcac
     9781 agctatgcct gttcatgggg ttctaggccg gaaccccaga tttttccgat tgtttgtttt
     9841 ctaactgatt attcagtagt tttagagggc tgacgctcag tggaacgaaa actcacgtta
     9901 agaggttttg actatgataa aagtcaaagt aggggtgagt ttggatatgg aaattattgt
     9961 actataagga gagcatgaaa acccctgggg cagtcactga atgagggacc agcagcggta
    10021 aaataaccag cataatttct gcatggtcga tttttatacc gtgacaggct gttttttata
    10081 cttggggtca gtttggatat cgaaaattat tgtacgttaa ggttgttttt tgaccttgtc
    10141 gatcttaact gatctcaaac gacgaaactt acctgaagtc ggtatccggc tgtttttcca
    10201 gatataatcg cctgtcagat tgatatgttc ccaacctaat ggtgataaat gcgataatag
    10261 ttgagcattt attttcatgc ccttacgctt cagagcatca accgctcttt ccatgtaaac
    10321 tgtgttccat aaggaaatag ccgccgtcaa taaagtcaat ccactggcgc gataactttg
    10381 attttccggt ttccgatctc taatctcccc caatcgatgc atgaatacag cacgggccag
    10441 agcattacgg gcttctcctt tgttcagccc ggcctggaca cgtcgccgca atgccggatc
    10501 ccgaaaccag tccagcataa acagtgttcg ttctatacgg ccaatctctc tcagagcttt
    10561 agccaaccca ttttgtttgt aagcgtgcag caagaaccgt attgacggga atgtgttatt
    10621 cagtcggcag tgctacgcgc caggggagca gttcgccgac ccggtttatc ggccagtcgg
    10681 ctatgacgtc aaggacatag cggaggtagc tttctggctc cactccgttc agtttgcacg
    10741 tcccgatcag gctgtacagc agcgctcccc gctctcctcc atgatccgaa ccgaagaaca
    10801 ggtagttttt gcggcccaga ctgaccatcc gcaacgcatt ttcagcgatg ttattgtccg
    10861 cctcagccca gccatcatct gcatagtacg tcagcgccgg ccactggttc agggcgtatg
    10921 cgaacgcttt cgccagttct gagtgtcgcg acagggtttt catcttttca cgcagccagc
    10981 tttccaggga tttcagcagc ggtttcgttt tcaactgacg ttcggcaagg cgctgttccg
    11041 ccgtcattcc ccttatctct gcctcgatgg cgtacagttc gccgatccgt ttcagcgctt
    11101 cctccgtcag ggctgacggg gtgcgaacgt gcacatcgtg gattttacgg cgggcgtgag
    11161 cccaacaggc ggcttccgtt atccggccat cccggtacag ctcgttgaac ccggcgtatg
    11221 catccgcctg cagtacacca ctgaaccccg caagatgggt ctgcggatgg atgccttttc
    11281 tgtccgggct gtaagcgaac cacaccgccg gcgccagcgt tgacccggcg ttacggtcgt
    11341 cacgaacata cgcccacaac cgcccggtct tcgttttctt attgcctggc aacagcaccg
    11401 ggacaggcgt gtcatcaaca tggagcttac cgtcagtcag cacatagcca tgaagcgcct
    11461 cttccatcgg agacagcagc cggcagcatg catccaccca gcccgacagc agtgaacggc
    11521 tcagctccac accttgccgg ccgtatattt ctgactggcg atacagcggg gtgtgctctg
    11581 catacttcga ggtcagcacg cgggccagca gccccggtcc ggcgataccc cgctcgatgg
    11641 gccgcgaagg tgcaggtgcc tgcacgatgg catcgcactg agtacaggca tgtttttccc
    11701 gtaccgtccg gataacccgg aaggcgctac gcatcaactc cagctgttcg gcggtatcct
    11761 cgcccagata gctcagtgaa ccgccgcagt tcgggcagca cggcgccgca ggcaacagtc
    11821 gcttttcgtc acggggtagt gattcaggga acggcttacg ggtgcgggtc tgacgcaacg
    11881 gacgctgtac tgccgggtca tacaccctac cagtcagcgt atcgctctct ttctgaagcc
    11941 ggttcagatc ggcttccatt tgtgcgatac ggcgggagac tttttcggaa cgactgccga
    12001 agttcatccg gcggagttta tccagctgcg cctgcagatg gtctatttcg cgctcc
//
LOCUS       DAQSWQ010000123        11808 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.124, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000123 DAQSWQ010000000
VERSION     DAQSWQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.124"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..488
                     /locus_tag="R8G18_003549"
     CDS             <1..488
                     /locus_tag="R8G18_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEE9797154.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="R8G18_003550"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="R8G18_003550"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEE9797155.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="R8G18_003551"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="R8G18_003551"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEE9797156.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="R8G18_003552"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="R8G18_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HEE9797157.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="R8G18_003553"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="R8G18_003553"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HEE9797158.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="R8G18_003554"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="R8G18_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HEE9797159.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="R8G18_003555"
     CDS             complement(6839..7747)
                     /locus_tag="R8G18_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9797160.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="R8G18_003556"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="R8G18_003556"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HEE9797161.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="R8G18_003557"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="R8G18_003557"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HEE9797162.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11808
                     /locus_tag="R8G18_003558"
     CDS             10715..>11808
                     /locus_tag="R8G18_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEE9797163.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3007 a         3001 c         2925 g         2875 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccga aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatagcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagctaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgcc
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacat
//
LOCUS       DAQSWQ010000124        11780 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.125, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000124 DAQSWQ010000000
VERSION     DAQSWQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.125"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..612)
                     /locus_tag="R8G18_003559"
     CDS             complement(<1..612)
                     /locus_tag="R8G18_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEE9797164.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRF"
     gene            complement(735..2381)
                     /gene="fumB"
                     /locus_tag="R8G18_003560"
     CDS             complement(735..2381)
                     /gene="fumB"
                     /locus_tag="R8G18_003560"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HEE9797165.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2459..3799)
                     /gene="dcuB"
                     /locus_tag="R8G18_003561"
     CDS             complement(2459..3799)
                     /gene="dcuB"
                     /locus_tag="R8G18_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HEE9797166.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4370..5089)
                     /gene="dcuR"
                     /locus_tag="R8G18_003562"
     CDS             complement(4370..5089)
                     /gene="dcuR"
                     /locus_tag="R8G18_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HEE9797167.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5086..6717)
                     /gene="dcuS"
                     /locus_tag="R8G18_003563"
     CDS             complement(5086..6717)
                     /gene="dcuS"
                     /locus_tag="R8G18_003563"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HEE9797168.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6898..7128
                     /locus_tag="R8G18_003564"
     CDS             6898..7128
                     /locus_tag="R8G18_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEE9797169.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7140..7412
                     /locus_tag="R8G18_003565"
     CDS             7140..7412
                     /locus_tag="R8G18_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEE9797170.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7639..7935
                     /gene="ghoS"
                     /locus_tag="R8G18_003566"
     CDS             7639..7935
                     /gene="ghoS"
                     /locus_tag="R8G18_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HEE9797171.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7963..8136
                     /gene="ghoT"
                     /locus_tag="R8G18_003567"
     CDS             7963..8136
                     /gene="ghoT"
                     /locus_tag="R8G18_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HEE9797172.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8255..9772)
                     /gene="lysS"
                     /locus_tag="R8G18_003568"
     CDS             complement(8255..9772)
                     /gene="lysS"
                     /locus_tag="R8G18_003568"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEE9797173.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10009..11466)
                     /gene="dtpC"
                     /locus_tag="R8G18_003569"
     CDS             complement(10009..11466)
                     /gene="dtpC"
                     /locus_tag="R8G18_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HEE9797174.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11525..>11780)
                     /locus_tag="R8G18_003570"
     CDS             complement(11525..>11780)
                     /locus_tag="R8G18_003570"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HEE9797175.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2858 c         2893 g         2985 t
ORIGIN      
        1 aaagcgccgt agctggcggc aatgaaagcg agcgaggaat atcaccgtcg ttaatgcgcc
       61 aagcagcgcg cagaacatat cggattgcgt gtcccactgg tcgccctggg tgccgagaaa
      121 atcatccgct ccctggccca ttgccagcgc cgcccaccac tcgattaatt catacatcgc
      181 gcttatcgcc agcgccacgc agcagactag gaacgccacc attttacgtc cgcgcacgta
      241 catcccgcgc acgagaattt ctcgtgccac cagtgcaggc accagcccct ggaaaaaatg
      301 ccccagcttg tcatacggat tacggctcaa ccccagccat tcctgcacct caaaaccgat
      361 ggggactttc gcgtaggtgt attgcccgcc gaccatcagg atgatggcgt gaaagaaaat
      421 gagcgtatag agcagcggcg ttaacggata acgtttagcg gtggcaagca gtagcggcac
      481 gacaataatc accggtgtca cttccatcag ccaggtgagt ttgtcatggg ccgaaatgcc
      541 ggtataaatc aggattaacg ttagcgccag tgcgccggtg ttaagaatta acggtttgag
      601 tgtgcgggtc atggtgagtt cgcaagtcag ggaaaatact gactattcac cagcaagcat
      661 gaaattacaa tcgcaaaaga ggtggcccag ggggatcacc tggcagcatg ctgccaggcg
      721 ctgggccgaa gaggttactt agtgcagttc gcgcactgtt tgttgacgat ttgctggaag
      781 aagtcgttac ctttgtcatc gaccaggata aacgccggga aatcttctac ttcgattttc
      841 cagatagctt ccatacccag ctccggataa gcgacgcatt ccagatgctt gatgctctgc
      901 tgcgccagta ccgccgccgg accgccgatg ctaccgaggt agaagccgcc gtgtttatga
      961 cacgcgtcgg taacctgctg gctgcggttg cctttcgcca gcatgatcat gctgccgccg
     1021 tgggattgca gcagatccac ataggagtcc atacgacctg cggtggttgg gccaagtgaa
     1081 cctgatggat aaccggcagg ggttttcgcc ggacccgcgt agtagatcgg gtgatctttg
     1141 atgtactgcg gaagttcttt accggcgtca atcagctctt tcagcttggc gtgtgcaata
     1201 tctcgaccca caataatggt gccagtgagc gacaaacgag tggaaaccgg atattgcgaa
     1261 agctgggcga ggatctcttt catcgggcgg ttaaggtcga ccttcaccgc ttcgccttca
     1321 ccggcctgac gcagttcttg tggaatgtac tgacctggat tgtgttccag tttttcgatc
     1381 cagatacctt cgcggttgat tttcgcttta atgttacggt cagcggagca ggagacgccc
     1441 atgcctaccg ggcaggatgc gccgtgacgt ggcagacgga taacgcgaat gtcgtgcgcg
     1501 aagtatttac cgccaaactg cgcgccaaga ccgagtttct gggcctcttc cagcagttcc
     1561 tgttccagct ggacatcgcg gaacgcctga ccatgttcgt tcccttccgt cggcagttca
     1621 tcgtaatagt gagcgcttgc taatttgacg gttttcaggt tggtttccgc agacgtaccg
     1681 ccaatcacaa acgcgatatg gtacggcggg caggctgcag taccgagggt acgcattttc
     1741 tcgacgagga agtttttcag tttgccggga gtcagcaggg ctttggtttc ctggtagaga
     1801 tacgttttgt tggcagagcc gccgcctttc gcaacgcaaa ggaatttgta ctcatcgcca
     1861 tctaccgcgt acaggtcgat ttgcgcaggc aggttagtgc cggtgttgac ctctttgtac
     1921 atgtccagcg ccgcgttttg cgagtagcgc aggttatctt cgatataggt gttatagacg
     1981 cctttcgaca gcgcttcttc atcaccgcca ccggtccaca cgcgctggcc ttttttaccg
     2041 acgatgatcg cggtgccggt atcctggcag gtcggcagca cgcctttggc ggcgatttcg
     2101 gagtttctta agaattgcag tgccacgtac ttgtcgtttt cgctggcttc tggatcgtga
     2161 agaatagccg caacctgttt ctggtgtgcc ggacggagca tgaaagaagc gtcgtgaaag
     2221 gcttgctgcg ccagcagggt cagggcttct ggttccactt tcaggatggt ttcgccgtcg
     2281 aagtcggcaa cgctaacgta atcggaagtg agtagatagt attcggtatt gtctttcccc
     2341 atcgggaaag gtgcctggta gataaagggt ttgtttgaca tagcttccag cctgtaactc
     2401 tgtatttgtt attcgaaaat ggcgtgccgc gtagtcacgg gcggcacgca aagtgcattt
     2461 ataagaaccc gtacatcgcg gcgaagatcc agccgaagac gcacgatacg ctcacaccaa
     2521 tcaaccccgg cagaataaag ctgtggttga tgacgaagcg accgatgtgg gtggtgccgg
     2581 aacggtcaaa ctgaatcgct gccagatcgc tcggataagt cggcaggatg taataaccgt
     2641 agcaagccgg tgctgaagcc acgatgtatg ccggatcaac gccgatcgcc agcgcgaccg
     2701 gaacaatcgc cgccagcgcc gcagcctgag agtttacaaa cttggaaacc agcagcagaa
     2761 caatggcata ggcccacgga tactctttca ccatttcacc cagtacgccc tgaatttcag
     2821 acatatgcgc accgaacatg gtttctgcca tccatgcgat accgtacacc gccacgatgg
     2881 cgatcatacc ggaacggaag acttcgtttt ttgagataga cgcgggattg gttttggtca
     2941 ggataataat cagcgccccg gtcagcagca taaacatctg aataaccagt accatcgaca
     3001 gcggtttgcc gccgaaggat ggacgcaggt ccgaatcagc accaagaagg gcgactacag
     3061 cgattgcccc gaggaaaatc cacattgcca gccagttgct tttcggcagt tttttgtcca
     3121 gcagcgtcgc ggtatcaccg taaacatact cacggttttc cggtacggag atgaatttct
     3181 ggaactcttc gtctttatcc agatctttac cacggaacca gctgaagata ccgatcgcca
     3241 ggataccgat taacgtcgat ggaatggtga ttgccagcag gtcgaggaac tcaagatggc
     3301 gaccatcaaa ggtgacatta cccagcattg caaccagaga cacgaccgca accgacaccg
     3361 gactggcgat gatccccatc tgtgcaccga tagaacttgc cgccatcgga cgttccggac
     3421 ggatgttgtt cttaatggcg acgtcgtaga tgatcggcag aatggtgtaa accacatgac
     3481 ccgtaccgca aagaatggtc agcgtacagg tcacaaacgg cgcgacaatt gagacatatt
     3541 tcgggttgcg gcgcagcagc ttctcggcaa tttgcagcat gacatcaaga ccgcccgaag
     3601 cttgcaaggt cgccgatgcc gccaccaccg caatgataac cagcatgaca tcaaccggtg
     3661 gtttacctgg ctgaaggtgg aagacgaaga ccagaatgac cagaccgata ccgcctaata
     3721 aacccagcgc gataccaccc tttctggcac cataaaacag acatatcagt attatgataa
     3781 gttggatagt aaataacatg tgtgaaccct cgcgataatc ctatttaaat ttttgctgaa
     3841 tagatcacag tcacgttctg ttttgtatga actgttttca gagcctgact aaatatccgt
     3901 ttggtcgtta ccggctttag caaatacctc acagtgaata ttggctgatt aaagcagcgg
     3961 caaatttatg cactgtgata agcggctttt tcagagagaa agactctctg gcgatgggtt
     4021 tatagtttgt actgcgcaga tgttaatgtt ttgtttgctg ttatcttatt gatatttatc
     4081 ggtctatttt ttgttctcag ggcagatgtc attaggttta tagattaatc tgatctaccc
     4141 atttgtgggt aaaaatacac ataatgcggg tgacataata gttaattaac ttttgttagc
     4201 gttttgaaat taaaaacacc gttcacctga agagatatta atttttagcg atgatgcagg
     4261 gataattata tttgatctgg cacaagtttt actgatgaag gatgtaactt gtgccagggg
     4321 tattttgcat tacggtaata attattactt acagataacc agcgatccgt tattggcaat
     4381 attgtttcag tagtgagtag tgttctgcct gaatacggta acggtaaact ggacgccccg
     4441 tgacgccata atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt
     4501 tacggcagga aacacgcgaa atgttaacct cgttggctag ctcgtcggtt gaaaattcat
     4561 agtcctgatg cgcgtcaatc cactggcaca gtgtgcgtaa cgtctgcggc gttaagcctt
     4621 ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct
     4681 cggcctggtc ataatactga tgtttttcca gcgccatttt cttttgccgc cagtcggtga
     4741 gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat
     4801 gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt
     4861 tgcaacgcgc gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga
     4921 tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct
     4981 ccagcgtcga ggctgttcca cagcattgaa agcctgggat ttgtgctacg tatcggcgat
     5041 tcagctccgc gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct
     5101 ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac
     5161 ggcgatgctg ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg
     5221 ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat
     5281 ccccggtcca tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac
     5341 gctaatttcg ccaccgggtt ccggccctaa tgcttccagc gcgttttcta tcagatttcc
     5401 caatgtggta atcagcgtcg cgacctggtc ctcactgccg ctgtctggca gctggctttc
     5461 actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcga ttaatcttgc tgattaaaaa
     5521 accagcgata accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt
     5581 attggctgtt tttagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc
     5641 gagaatcacg tgcaatttat tcataaattc gtgggatcgt tcacgaagtg cgtcggcata
     5701 gttgaccaga ccgtcgagtc gctgcatcag tttacgtact tcggttttgt ccctgaaggt
     5761 tgaaatggca ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag
     5821 ccggtcttta atcgtaatct cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga
     5881 gacatctacc acctgtgacc atgagtggct gagcgtcgac agtttctcat cgtcctgcga
     5941 cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc
     6001 gtccacggca acgacgcctt ctttgataga ctgcaacatg gcctggcgtt gctcaaacag
     6061 cgtggagatt tcgtagggtt ccaggccgaa aaggattttt ttcagtacct taaccagaat
     6121 gcaggtgcca atcagtccga ccagcatgcc aaataatacc gaccagataa tgctccagcg
     6181 actgtcattg atttgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat
     6241 ttgtttatga ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc
     6301 gcgattgata gcgacatttt ctttgccatt cagcgcttta aggatgtcat cacctttaaa
     6361 tggctgacca atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac
     6421 gacaataaac agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggatgccgct
     6481 ctcctgcggt tttttctgca agccctgacg gatttccggc gagttggcga gggtacgcgc
     6541 cactgccagt gccttgttgg ctagcccgtc tcgcgtcata tcactgattt gcgagaagta
     6601 aatcagatgc accaccaata gcaccgagaa cagtaccgca ctgaccatta agatcactgt
     6661 ggtactcaat ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag
     6721 cttccttgtg tgacaaattt cttaaacatt atctctgatg aggcgggtaa ttcaaaggga
     6781 gtaagaatga ttggctatat aggggaagag attctggcaa cggaaactgc cagtgctgta
     6841 tgaagattcc agggctatgc ttatagcgat aatcatactg atgagagagg gaaagtcatg
     6901 gatcaggcgc tactggacga gggttatcgc tgttataccg gcgaaaagat cgatgtctat
     6961 ttcaacactg cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt
     7021 aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg acgtcgccac tgtggttaaa
     7081 gtgattgata cgtgcccgag cggcgcgctg aaataccgtc ataaataagc gagggtaaaa
     7141 tggaaatacg cgaaggccac aataaatttt acattaatga cgaacaaggc aagcaaatcg
     7201 ctgaaattgt ctttgtgccg actggagaga atttagcgat tatcgaacat accgatgtcg
     7261 atgaaagcct gaaagggcag gggattggta aacagctggt tgcaaaagtc gtggaaaaaa
     7321 tgcgtcggga aaaacgaaaa attatcccac tatgcccatt tgcgaaacac gaatttgata
     7381 aaacgcggga gtatgatgat attcgcagtt gatgggagag tacagagtca cgatattttt
     7441 catgctctcc gcggagtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat
     7501 gggcagagag cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata
     7561 tctcctatat gagaatcatc aatcggggtt atcaagtttt gcgtccccag agcattcaat
     7621 attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt tgtttctttt
     7681 gattatccat catcttattc ctcagtgttc ttaaggttaa gatcattgat gtatgatatg
     7741 aatttctctt ctatcgtggc tgatgaatat gggataccac gacaattgaa tgaaaattcc
     7801 ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag gcttaaatgc
     7861 ttagacttac cggatattga ttttgacctc aacattatga cagttgatga ctatttccgt
     7921 cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact attctctaaa
     7981 atattaattt tttatgtgat tggtgtgaac atatcctttg tcattatctg gtttatctca
     8041 catgagaaaa cacatattcg tttacttagt gcattcctgg tcggaataac ctggccaatg
     8101 agtctgcctg tggcattact tttttctctc ttttaggagc tgtagttgag tggacccgct
     8161 ggttatcgtg aaacgaagcg tctctcctca aaactggatt tgcggaacga cgaacgaagc
     8221 agtcgcctga ctgcttcgtt cattaaagtg aaatttattt ctgtgggcgc atcgccggga
     8281 agagaataac gtcgcgaata gtatgactgt ttgtaaacag cataatcatt cggtcgatac
     8341 caatacccag accagcggtt ggcggcagac catattccag cgcagttacg taatcttcgt
     8401 catagaacat ggcttcgtcg tcacctgcag ctttagcgtt aacctgttcc tggaaacgtt
     8461 cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt accgatttca cgaccaccga
     8521 tgaagaattc aaaacggtcg gtgatttccg ggttaacatc attacggcgc gccagcggtg
     8581 acacttctgc cggatattcc gtaataaagg ttggctgaat cagatgtgct tctgccactt
     8641 catcaaagat ctccgtgata atacgtccca gcccccagct tttctctacc gtaattccga
     8701 tagattcagc taatgcttta gcagcatcaa aattatccag gtcggccata tcggtttctg
     8761 gacgatattt tttgattgct tcgcgcatgg tgagtttttc aaacggtttg ccgaaatcaa
     8821 acacatgctc gccataagtg actttagtgg tacccagaac ctcttgtgcc agggtgcgga
     8881 acagtgactc tgtcagttca atcaaatcgt ggtaatccgc atacgccatg tagagttcca
     8941 tcattgtgaa ctcaggatta tggcgaacag aaataccttc attacggaag ttacggttga
     9001 tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt cagatacagc tccggcgcga
     9061 tacgcagata catatctaaa tccagagcat tatgatgggt aataaacggg cgagcagatg
     9121 ccccacctgg aattacctgc atcatcgggg tttctacttc cataaagccg cgcgcgacca
     9181 tgaactgacg gatagcggcc agaatttttg aacggacaac aaacgtttga cgggatttat
     9241 cgttagcgat gaggtccaga taacgttgac gataacggac ttcctgatcc tgcagaccat
     9301 ggaatttatc tggtaaagga cgtagtgctt tagtcagcag gcgcaactca gtacagtgaa
     9361 tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg ggcaccgata atgtcaccca
     9421 gatcccattt tttaaactga tcgttataaa caccttctgg caggctatct cttgcaacgt
     9481 acagttgaat acggccaccg acatcctgca acgttacaaa ggaggctttc cccatgatac
     9541 gacgggtcat cattcggcca gcaaccgaga cttcaatgtt taaggattcc agttcctggt
     9601 tattcttcgc atcaaactct tcgtgcaact ggtcagaggt atggtcgcgg cgaaaatcat
     9661 tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt ttcgcggcga tttctcagtt
     9721 catcgttaaa atcaatagcc tcattggctc cccgtgtttc ttgttcagac attttggttc
     9781 ctctaaatcc agctttcaat ttagcgtaga taaagagaca gatcggtaca ctgtattatc
     9841 tgcctcgact attaataact cagtttatct tatcgaaact aacgcttacc tcaagttgat
     9901 atcactaata aataaccatc aaaatggttt aaatgactta ttaatctcga tttactagtg
     9961 tgattaactg aaagttaatt tattcataac caattgtttt tacgacagtt aatcgttgct
    10021 ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg gtagcaaagg ccaatacgac
    10081 gatgatcgcg acacaagcca acgtccactc tcccatctga gaaaagaatc gctggtaagc
    10141 tgcaattacc ataccgctaa tttgcgactc tgtcgtctgc tgtgccacga cgcctgccag
    10201 ccagttggcg accgcgcctg ttgccagcat ataaataccg gttaatacgc cagacatttt
    10261 cagacgcgtg atttgcgcaa tcgccaccgg gtcaataaag agttctgcaa agcccattag
    10321 cgccaacccg gatatcatca cgcccattga cgcttgaccg tcagccgctg catgtcgggc
    10381 atcaaatgcc aacaacataa agccacaagc catcagcagt aagccaaagg caaacttcag
    10441 ccagacgcgc aatgttgagt tgccgcggct ttctggcgac gccagccagg ccagtacaac
    10501 cccagcgagc atcactgcaa ttgcattcac cgactggaat agtgctgtag gtacttcaat
    10561 attgaatgcc tgacgattca caaagcgatc gataaacaag ctgatggtac tgccgccctg
    10621 ttgtgccagt acccagaaca acgtcccgac aaacatcaac aatacaattt gccaaagagc
    10681 acggcggtgt tcggggaatt tgatcatcat gcgggcaatg atttgtgcgg caatgaggca
    10741 aacgatcgcc agcaaatatc cggaccagtc gttctccagc agcagggtaa aaaatactgg
    10801 ggctaaacag agcatcacca ctaaccagct ccatactggt aaggcaaatt tgacgctagt
    10861 gagcgctttt ttatccatac tacgcgtgga ctggaaatga cgatgaccgc ttaagaaaat
    10921 caacaaaccg ataaacatgc cgccacccgc aagggcaaag ccaacatgcc atccatacca
    10981 ctgagcagcc aggccgcagg cgattggggc ggcgatagaa ccgatattgc ccgcagcata
    11041 cagcagcgaa aaaccgccat cacgtcgatg atcgttctcg tcgtagagct cgccaagcaa
    11101 acagctgatg tttgatttga ataaaccgta gccacaaata atgattgcca gcgccagata
    11161 caggctaaag gttgaatttg tatcaatgcc cagcaccaca tggccaaggg tcattaacag
    11221 cgcgccggca atcactgcag tgcggttgcc gagcaggcgg tcggcaagcc agccgccgag
    11281 aataggggta acgtaaacca gagaagcata tgcgctgaac aggctgatgg catggttatc
    11341 atcaaaacca agctgatggg tgagatagag aatgagtaag gcacgcatgc cgtaaaaact
    11401 gaagtactcc cagatttgga tcgccacgat atagtatatc gcgcgcggct gtgagggtgt
    11461 tttcatgtgt tctccttatg agcaaaaaag ggaagtggca agccacttcc cttgtacgag
    11521 ctaattattt tttgctttct tctttcaata ccttaacggt atagcggcca tcagcctgac
    11581 ggtatgcacc gtgaatatcg gtttcaaatc ccggatagtg agcgccgatt tcacacagca
    11641 tctgcaggaa ctccagaacc ggacggcttt cttcggtgat catttcaccc ggcattacca
    11701 gaggaactcc cggcggatac ggaaggatca tattggcgtt aatacgacct accatttcgt
    11761 cgaggtaaac ttcttcggtc
//
LOCUS       DAQSWQ010000125        11698 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.126, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000125 DAQSWQ010000000
VERSION     DAQSWQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.126"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(350..1603)
                     /locus_tag="R8G18_003571"
     CDS             complement(350..1603)
                     /locus_tag="R8G18_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9797176.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="R8G18_003572"
     CDS             1710..2603
                     /locus_tag="R8G18_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9797177.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="R8G18_003573"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="R8G18_003573"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HEE9797178.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="R8G18_003574"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="R8G18_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEE9797179.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="R8G18_003575"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="R8G18_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HEE9797180.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="R8G18_003576"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="R8G18_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HEE9797181.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="R8G18_003577"
     CDS             complement(6839..7693)
                     /locus_tag="R8G18_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HEE9797182.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="R8G18_003578"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="R8G18_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HEE9797183.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..10746)
                     /locus_tag="R8G18_003579"
     CDS             complement(8323..10746)
                     /locus_tag="R8G18_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HEE9797184.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(10807..>11698)
                     /locus_tag="R8G18_003580"
     CDS             complement(10807..>11698)
                     /locus_tag="R8G18_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEE9797185.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2960 a         2965 c         3020 g         2753 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagggccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccacaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt ccgtaccatg
     9421 gtcgatggca tcagtccatg taaagacgga aatctgcgtt ttaacaggat tctcgagcat
     9481 cgggaaccat tctaccccca gatcccagct accttcgcgt acgcctgagt tgccgccgtt
     9541 tatgccgacg ttaccggtga gaacggaaag catggcaata gcgcgggatg tttgttcgcc
     9601 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
     9661 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
     9721 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
     9781 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
     9841 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
     9901 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
     9961 acgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    10021 agtgacacca ccaccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag
    10081 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    10141 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac caccgcaaga
    10201 gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta cacctgttcc
    10261 gtacaggaca tgtacagctt cattgccgta atcttttagg atccgccgaa gattatcact
    10321 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgcttgcc
    10381 gacgcgcttc atgggatatt tcaacctatc aggatgattc atccgtcggc ggatagagcg
    10441 cccgcgtaaa cacgcgcgaa cctgatgatt accgtagacg tcgtcacctg tcgtatcata
    10501 ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc ggctcccgca
    10561 gttaacggtg caggaactcc agaccgcttt ctcttctacc ggagcctctg ccgcccgaac
    10621 catttgggaa aatggcagag tgaaagcact gcttgccagc gcaaggctgc caagtgcgga
    10681 ggttttcatc agacttctac ggctgatttc agccttcatg agcgcctctg tggtatggat
    10741 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    10801 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    10861 tggtgaaggg cgcagagtgg ttagcgtatt cacacagccg ccatggtcga ttttatcgcc
    10921 agacatattg gcctcgtgcc aggctccctg gcccatagcg ctaaccccag ggagaatacg
    10981 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    11041 gccggtggca atcccacgtt tctgtgcatc tatagggttg atccacactt cctgacggca
    11101 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    11161 accaaacagt tgcagtggga aggtactacg ttcaggggag tcccagcctt caaaggttga
    11221 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    11281 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    11341 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatga ccattgggat ctttacgctt
    11401 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    11461 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttcagtga acttttgata
    11521 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttgcgttc
    11581 gaatttttcg ctggtgacag gctggaggaa aatgagatat cccatgttac cggcgtagtc
    11641 gttaggaata atatcttcct gctcaacggt catcaggtct ggcagcagaa tgtcggca
//
LOCUS       DAQSWQ010000126        11542 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.128, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000126 DAQSWQ010000000
VERSION     DAQSWQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.128"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            460..1386
                     /locus_tag="R8G18_003581"
     CDS             460..1386
                     /locus_tag="R8G18_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEE9797186.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="R8G18_003582"
     CDS             complement(1336..2529)
                     /locus_tag="R8G18_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9797187.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="R8G18_003583"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="R8G18_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HEE9797188.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="R8G18_003584"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="R8G18_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HEE9797189.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="R8G18_003585"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="R8G18_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HEE9797190.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="R8G18_003586"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="R8G18_003586"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HEE9797191.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="R8G18_003587"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="R8G18_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HEE9797192.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="R8G18_003588"
     CDS             complement(11165..11308)
                     /locus_tag="R8G18_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797193.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2926 a         2896 c         2950 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAQSWQ010000127        11469 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.129, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000127 DAQSWQ010000000
VERSION     DAQSWQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.129"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..611
                     /locus_tag="R8G18_003589"
                     /pseudo
     CDS             1..611
                     /locus_tag="R8G18_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="R8G18_003590"
     CDS             608..1222
                     /locus_tag="R8G18_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HEE9797194.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="R8G18_003591"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="R8G18_003591"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HEE9797195.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="R8G18_003592"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="R8G18_003592"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEE9797196.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="R8G18_003593"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="R8G18_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HEE9797197.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="R8G18_003594"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="R8G18_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HEE9797198.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="R8G18_003595"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="R8G18_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HEE9797199.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="R8G18_003596"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="R8G18_003596"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEE9797200.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="R8G18_003597"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="R8G18_003597"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEE9797201.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDCG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="R8G18_003598"
     tRNA            9115..9190
                     /locus_tag="R8G18_003598"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="R8G18_003599"
     CDS             9796..9954
                     /locus_tag="R8G18_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797202.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2970 a         2668 c         2893 g         2938 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gatctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatggcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggactgcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcacaa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga ttaaaatata aacagataat tatgtatctt attgatttat
    10201 aaggttattt ttaagtaaag gtaaataaaa aataaatagc cataaatagc tataaataca
    10261 tcttaatacc tatatccctt cccttatggc tagattgcga gaggtgctta acgcgatttt
    10321 cagcgccccc cgatctggaa aatccagaat ccccaaatat cagcaaggaa ttttcttgcg
    10381 taatcaacat cgtttttacc tattaaatca ttacacagaa agcctttctg taatgtctct
    10441 cactaaactt tcactaaaca cgagaatgat cggttttatg tcggatcaga tgctagaaac
    10501 agcaccacgt ctcacaaggg ctgtaagcga tgaaaccagt gtttatgcgg gtgcaggtca
    10561 aaatataggc caaaatccac ttaacataat aaatgttata tgtgctaagg aacacatata
    10621 acgccttgaa ttgatgtatc aggggaaaag tgatggattt tttacgtagg agcacgaaag
    10681 ggcttctatt gacttttgta tgctatgaaa tataattaaa gttttttgtt tttattgtgt
    10741 gatgtggtgg gttacacagg ggaatagatt aaatggtgtt ctgtttatac aggtttattt
    10801 gttagatcaa caatttggca tttctaaaca gatgtttgaa cgttgccaaa tgtcttgaca
    10861 acacaagaaa agggcttgct atgccaggca ttacacgaaa taaggctata aaaacaaaat
    10921 aaattttaaa atttgcacaa acgagaaact cgttcagaaa caagcagaac aatttttagc
    10981 caacaatttc tgatggtgct ctgtaagccg cattacacaa ggctttgcga aagtgatgta
    11041 aaagtgaaga aagtaagaat tgaactttta agctactttt cattggattt tttagtaggg
    11101 aattacacag ggaatagaga gcattattct atgatttaca cagggaatat agggggatta
    11161 tggcacacac atagaaagac attacacaga gtgctatgtg acaaatggat gtaacgagag
    11221 gttacattca tttatctgaa atgctctctg taagaaagtt ggtagtaacg atatgttaca
    11281 tagactaatt attgaatgta acgatattac acatcggtag taacgatacg ttacatcgga
    11341 agatgattgg atgtaacggt agctcacagt gaactagggg taggtcatag tgaactaccc
    11401 ccacagaatt ctgttacccc tatcaagtct gataccccta tcaaaactga tacccgataa
    11461 gtataaata
//
LOCUS       DAQSWQ010000128        11428 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.130, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000128 DAQSWQ010000000
VERSION     DAQSWQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.130"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="R8G18_003600"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="R8G18_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEE9797203.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="R8G18_003601"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="R8G18_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEE9797204.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="R8G18_003602"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="R8G18_003602"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEE9797205.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="R8G18_003603"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="R8G18_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEE9797206.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="R8G18_003604"
     CDS             complement(3928..5214)
                     /locus_tag="R8G18_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEE9797207.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="R8G18_003605"
     CDS             complement(5235..6026)
                     /locus_tag="R8G18_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEE9797208.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="R8G18_003606"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="R8G18_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEE9797209.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="R8G18_003607"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="R8G18_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEE9797210.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="R8G18_003608"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="R8G18_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEE9797211.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="R8G18_003609"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="R8G18_003609"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEE9797212.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="R8G18_003610"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="R8G18_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEE9797213.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="R8G18_003611"
     CDS             complement(9882..10364)
                     /locus_tag="R8G18_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEE9797214.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="R8G18_003612"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="R8G18_003612"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HEE9797215.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAQSWQ010000129        11246 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.131, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000129 DAQSWQ010000000
VERSION     DAQSWQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.131"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..1760
                     /locus_tag="R8G18_003613"
     CDS             <1..1760
                     /locus_tag="R8G18_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEE9797216.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="R8G18_003614"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="R8G18_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HEE9797217.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="R8G18_003615"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="R8G18_003615"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEE9797218.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="R8G18_003616"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="R8G18_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEE9797219.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="R8G18_003617"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="R8G18_003617"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEE9797220.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="R8G18_003618"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="R8G18_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HEE9797221.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="R8G18_003619"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="R8G18_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HEE9797222.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="R8G18_003620"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="R8G18_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HEE9797223.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="R8G18_003621"
     CDS             complement(10054..10212)
                     /locus_tag="R8G18_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEE9797224.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="R8G18_003622"
     CDS             10395..10694
                     /locus_tag="R8G18_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9797225.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="R8G18_003623"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="R8G18_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HEE9797226.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
BASE COUNT         2786 a         2785 c         2939 g         2736 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtgag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcg
//
LOCUS       DAQSWQ010000130        11242 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.132, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000130 DAQSWQ010000000
VERSION     DAQSWQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.132"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..309
                     /locus_tag="R8G18_003624"
     CDS             <1..309
                     /locus_tag="R8G18_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEE9797227.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="R8G18_003625"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="R8G18_003625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HEE9797228.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="R8G18_003626"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="R8G18_003626"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEE9797229.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="R8G18_003627"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="R8G18_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HEE9797230.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="R8G18_003628"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="R8G18_003628"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEE9797231.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="R8G18_003629"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="R8G18_003629"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEE9797232.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="R8G18_003630"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="R8G18_003630"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEE9797233.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="R8G18_003631"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="R8G18_003631"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HEE9797234.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /locus_tag="R8G18_003632"
     CDS             complement(8117..9763)
                     /locus_tag="R8G18_003632"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HEE9797235.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /locus_tag="R8G18_003633"
     CDS             complement(9766..9816)
                     /locus_tag="R8G18_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797236.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="R8G18_003634"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="R8G18_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HEE9797237.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..>11242
                     /locus_tag="R8G18_003635"
     CDS             10354..>11242
                     /locus_tag="R8G18_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEE9797238.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2553 a         3091 c         2951 g         2647 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcaccc
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAQSWQ010000131        11079 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.133, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000131 DAQSWQ010000000
VERSION     DAQSWQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.133"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="R8G18_003636"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="R8G18_003636"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEE9797239.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="R8G18_003637"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="R8G18_003637"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEE9797240.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="R8G18_003638"
     CDS             complement(2698..3390)
                     /locus_tag="R8G18_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEE9797241.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /locus_tag="R8G18_003639"
     CDS             3520..5280
                     /locus_tag="R8G18_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEE9797242.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5686..6543
                     /gene="focA"
                     /locus_tag="R8G18_003640"
     CDS             5686..6543
                     /gene="focA"
                     /locus_tag="R8G18_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HEE9797243.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6598..8880
                     /gene="pflB"
                     /locus_tag="R8G18_003641"
     CDS             6598..8880
                     /gene="pflB"
                     /locus_tag="R8G18_003641"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEE9797244.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9072..9812
                     /gene="pflA"
                     /locus_tag="R8G18_003642"
     CDS             9072..9812
                     /gene="pflA"
                     /locus_tag="R8G18_003642"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HEE9797245.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9894..10484)
                     /locus_tag="R8G18_003643"
     CDS             complement(9894..10484)
                     /locus_tag="R8G18_003643"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HEE9797246.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            10584..>11079
                     /locus_tag="R8G18_003644"
     CDS             10584..>11079
                     /locus_tag="R8G18_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9797247.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKL"
BASE COUNT         2861 a         2782 c         2593 g         2843 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcctgatgc
     5461 tcccttttaa ttaactgttt tagcggagga tgcggaaaaa attcaactca tttgttaatt
     5521 tttaaaattt atttttattt ggataatcaa atatttactc cgtatctgca taaaaaccat
     5581 gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat
     5641 gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac
     5701 ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat
     5761 aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc
     5821 tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc
     5881 atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc
     5941 ggagccgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag tgggcgcatc
     6001 acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca
     6061 ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg
     6121 ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc
     6181 tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc
     6241 cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc
     6301 ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac
     6361 ttcgcatccc cggaattttg gaccgcagtc ggttctgcac cggaaaattt ttctcacctg
     6421 accgtgatga atttcatcac tgataacctg attccggtta cgatcggcaa cattatcggt
     6481 ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgaccaccat
     6541 taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg
     6601 tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag
     6661 aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag
     6721 tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc
     6781 gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc
     6841 atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt tggtctgcag
     6901 actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt
     6961 tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt cactgaatac
     7021 cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt
     7081 aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat catcggtgac
     7141 taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact ggcacagttc
     7201 acttctctgc aggctgatct ggaaaacggc gtaaacttgg aacagactat ccgtctgcgc
     7261 gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac
     7321 ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc
     7381 ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc
     7441 accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa
     7501 gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact
     7561 ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt
     7621 atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg
     7681 tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg
     7741 ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag
     7801 aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctattgc ttgctgcgta
     7861 agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa
     7921 accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg
     7981 aagtctgaac cgatcaaagg cgatgtcctg aactatgatg aagtgatgga gcgcatggat
     8041 cacttcatgg actggctggc taaacagtac atcactgcac taaacatcat ccactacatg
     8101 cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc
     8161 accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa
     8221 tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa
     8281 ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc tgttgacctg
     8341 gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact
     8401 cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaacggg taacacccca
     8461 gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac
     8521 cagaaaggtg cagtagcctc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa
     8581 gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt
     8641 cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa
     8701 ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa
     8761 aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac
     8821 tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa
     8881 ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg
     8941 ccttttttag cacgagagcc ttttttgtca gctatctata ctctaaggtg actgccaaaa
     9001 cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga
     9061 gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag
     9121 acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt
     9181 gtcataaccg cgacacctgg gatacgcatg gcggtaaaga agttaccgtt gaagatttaa
     9241 tgaaagaggt ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat
     9301 ccggcggtga ggcaatccta caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa
     9361 aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga
     9421 ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg
     9481 agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc gccaaatatc
     9541 tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg
     9601 atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac gttgagaaaa
     9661 tcgagcttct cccctaccac gagctgggca aacacaaatg ggtggcaatg ggtgaagagt
     9721 acaaactcga cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg aaaggcattc
     9781 ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca actttattga
     9841 gttgccgctt ttttattccg cacttcattc tgctacagcg tttttaacac atcttatagt
     9901 gcatctacca tatcccgtac ctgagataat aacgactgat aatagctcgc gtgaagttct
     9961 tccccatcga ataccactgt attacacaag aaagtgacat cggcatcttc aataccaaga
    10021 taactgcaca tattttttaa ataatcgctt atattttttt cccagcccat ctggacaaat
    10081 gattctttgt ctcctccaac cagcgccacc caacgaactt tattgaatgg taatttctgc
    10141 ccatctccat aagccagccc attattccat actctgtcaa tatatccttt tagcattgcc
    10201 gggaagctgt accaccagac aggaaaaacc accactaacg tgtcatgttc aagcagctct
    10261 gaataaagct gatgaacctc tggagaataa cgtttatcca tattcttcca gtctggttca
    10321 tcttccggcg tcatcaccgg gttgaaatta cgccgatata aatcgagttc cgtcacctgt
    10381 attttccgct ccattgcccg ctgatggatc gcatcagcaa tatgtgcggt caatgaatca
    10441 tgacgaggat gggcccatac caaataaata cgttcagact gcataatttt acctccacgt
    10501 taagttctgg agcgcacttt acgcaggcgg cataatcatg taaacgtgat gattcaggat
    10561 gagttaatga aggattttca ctaatgcgga tgaatatgtc tgactttgcc actttctttg
    10621 ctgtggcccg taatcaaagc tttcgtgcag cgggcgatga gttaggctta tcctcgtccg
    10681 ccattagcca tagtattaaa acactggaac aacgtcttaa aattcgctta ttcaatcgga
    10741 caacccgaag cgtatcgtta acggaagcgg gttctaatct ctatgaacga ttgcgcccag
    10801 ctttcgacga aattcagatc atgttagatg agatgaatga ttttcgtctg acaccaacag
    10861 gaacgctcaa aatcaatgcc gcccgggtag ctgcacgtat atttttaatg cctttactgg
    10921 ttggtttcac acgtgaatat cctgatatta aagtcgagct cacaactgac gactctcttg
    10981 tcgatatcgt ccaacagggc tttgacgctg gcgttcgact tagtggcatc gtcgaaaaag
    11041 atatgatcag cgtggcgatc ggcccaccag tgaaattat
//
LOCUS       DAQSWQ010000132        10939 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.134, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000132 DAQSWQ010000000
VERSION     DAQSWQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.134"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            108..605
                     /gene="ftnA"
                     /locus_tag="R8G18_003645"
     CDS             108..605
                     /gene="ftnA"
                     /locus_tag="R8G18_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HEE9797248.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(643..882)
                     /locus_tag="R8G18_003646"
     CDS             complement(643..882)
                     /locus_tag="R8G18_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HEE9797249.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1073..2284
                     /gene="tyrP"
                     /locus_tag="R8G18_003647"
     CDS             1073..2284
                     /gene="tyrP"
                     /locus_tag="R8G18_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HEE9797250.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="R8G18_003648"
     CDS             complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="R8G18_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HEE9797251.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3207..3293)
                     /locus_tag="R8G18_003649"
     tRNA            complement(3207..3293)
                     /locus_tag="R8G18_003649"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Leu,seq:taa)
     gene            complement(3306..3379)
                     /locus_tag="R8G18_003650"
     tRNA            complement(3306..3379)
                     /locus_tag="R8G18_003650"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Cys,seq:gca)
     gene            complement(3434..3509)
                     /locus_tag="R8G18_003651"
     tRNA            complement(3434..3509)
                     /locus_tag="R8G18_003651"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3474..3476),aa:Gly,seq:gcc)
     gene            complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="R8G18_003652"
     CDS             complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="R8G18_003652"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEE9797252.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="R8G18_003653"
     CDS             complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="R8G18_003653"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEE9797253.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="R8G18_003654"
     CDS             complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="R8G18_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HEE9797254.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7047..7223
                     /locus_tag="R8G18_003655"
     CDS             7047..7223
                     /locus_tag="R8G18_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797255.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7210..7434
                     /locus_tag="R8G18_003656"
     CDS             7210..7434
                     /locus_tag="R8G18_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HEE9797256.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="R8G18_003657"
     CDS             complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="R8G18_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HEE9797257.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8453..9205)
                     /gene="yecC"
                     /locus_tag="R8G18_003658"
     CDS             complement(8453..9205)
                     /gene="yecC"
                     /locus_tag="R8G18_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HEE9797258.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="R8G18_003659"
     CDS             complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="R8G18_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HEE9797259.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="R8G18_003660"
     CDS             complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="R8G18_003660"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HEE9797260.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         2713 a         2838 c         2578 g         2810 t
ORIGIN      
        1 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
       61 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
      121 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
      181 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
      241 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
      301 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
      361 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
      421 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
      481 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag
      541 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
      601 actaatgctc tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt
      661 actctgatcg gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa
      721 cgctaccagc tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
      781 accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc
      841 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
      901 gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt
      961 tattgtacat ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc
     1021 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     1081 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     1141 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     1201 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     1261 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     1321 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg
     1381 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     1441 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     1501 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     1561 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     1621 tctgtcagca atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat
     1681 tgtcagctat atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc
     1741 gatccccctg gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac
     1801 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     1861 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
     1921 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca
     1981 gcgttcaaat accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt
     2041 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     2101 gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa
     2161 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     2221 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
     2281 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg
     2341 gcctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg
     2401 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
     2461 gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca
     2521 aacgcttctg gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca
     2581 atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg
     2641 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
     2701 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
     2761 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc
     2821 ggcacatagt cagccccacc ccacacggca accagccact gttccggttc aatctcttgc
     2881 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
     2941 ttagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
     3001 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
     3061 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
     3121 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
     3181 ctttgatttt attcttatct ttcccatggt acccggagcg ggatttgaac ccgcacagcg
     3241 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
     3301 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
     3361 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
     3421 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
     3481 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
     3541 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
     3601 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
     3661 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
     3721 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
     3781 ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
     3841 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
     3901 aatttcacgg gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc
     3961 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
     4021 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
     4081 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
     4141 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
     4201 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
     4261 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
     4321 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
     4381 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
     4441 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
     4501 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
     4561 ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt
     4621 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
     4681 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
     4741 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
     4801 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
     4861 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
     4921 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
     4981 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
     5041 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
     5101 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
     5161 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
     5221 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
     5281 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
     5341 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
     5401 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
     5461 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
     5521 tcaacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
     5581 cgccctatct gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac
     5641 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
     5701 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
     5761 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
     5821 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
     5881 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
     5941 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
     6001 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
     6061 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
     6121 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
     6181 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
     6241 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
     6301 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
     6361 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
     6421 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
     6481 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
     6541 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
     6601 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
     6661 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
     6721 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
     6781 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
     6841 tggtgggtat tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac
     6901 gcccgtcacc aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa
     6961 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
     7021 ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg
     7081 catttaatag cattgttgac gtcacgctca aaaaacaacc actgctattt ttagcaaagc
     7141 ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga
     7201 ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat
     7261 gaagtctcct gcctgaaagc gatgctgacg ctgatgctac aggcgatggg acaagctgac
     7321 gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag
     7381 gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa
     7441 accggctgag agcgtgcctt tcagccgatt tttgcatctg gcacgcagaa agaaaagcga
     7501 tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat
     7561 ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat
     7621 cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt
     7681 gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag
     7741 actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc
     7801 gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata
     7861 ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc
     7921 gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac
     7981 aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact cagggtaatt
     8041 ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata
     8101 gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc
     8161 ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg
     8221 catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc
     8281 acataaataa gatttatata taatttatat tattcaggca atgaattact tttgcaagcc
     8341 atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct
     8401 tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca
     8461 gcagaaactt ctcgaggaac tggcgggtgc gaggctgctg gggatcagca aataacgctt
     8521 ttgctgcccc ctgctcgact atccgcccct ggtccataaa aatcgcccgg tccgcaacat
     8581 cccgggcaaa gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct
     8641 ggcggattgt gttcaggact tcgcccacca gctctggatc cagcgctgac gttggctcgt
     8701 caaacaaaat cacctcagga cgcattgcca gcgcacgcgc aatcgctaca cgctgctgtt
     8761 gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca
     8821 gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc
     8881 cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa
     8941 acccgacgtg ctgacgcaac tggcggatca gcgatttttg ctgacttaat gaacgtgcag
     9001 tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta
     9061 tgctgcgtag caacgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc
     9121 caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat tttttcacca
     9181 ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc
     9241 aaaatggttc tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc
     9301 caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag
     9361 ctccggcacc tggattgtcg cggccagcga cgtatccttt accaggctga taaagctgtt
     9421 cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatagtctg
     9481 ccatggcgtc ataccaatac tggcagctgc ttcccactga cctttatcaa tagaagaaat
     9541 ggcggcgcgc agtgtttcgg cggcataggc ggcagtgttc agcgacagac caatcattgc
     9601 tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag
     9661 ttgtgcgatg agcggtgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac
     9721 gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagcagta agccaaaaaa
     9781 catgccgccg atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga
     9841 atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac gtggggatga
     9901 taggcgaaca gcgcaggcgc accgccggta tgaataaaca gaatcggccc ttcatctttg
     9961 aagcgtttct gactgataca gccaatcagc cccgccatcg cttttccggt atacacagga
    10021 tcaagcagaa tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc
    10081 ggcacgccgt agccaggtgc aaaatagtca tcccagagta taatttccgc tgatgcggtc
    10141 agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ctgatcggca
    10201 acggaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca
    10261 acagccagtc cggcgtgagt tccggcactg cccgatgcga ctaccaccga cgaaatatta
    10321 accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgcca
    10381 agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc
    10441 gtcgccagct cttccagttg ggcattggga tcggtcagtg cttcgcacat ttcaatctgg
    10501 gtattgaaca gatccagcaa caagcgattg ccgttggtta aatagttttc tgcggttgtg
    10561 ccaataggat tttccagcag cgccacacag tgcagaccga gtttcgccgc aactgcggca
    10621 gtctgccgca catggttaga ctggatcacc ccggcagtaa tcagcgtatc ggcaccttcg
    10681 cgcagagcat ctgccgcgag aaattctagc ttacgtaatt tattgccgcc cattgccatg
    10741 ggggtgacgt catcccgttt gatgaaaatt tcccgtccta gataatcaga aaagcgcggc
    10801 agatattcga gcggcgttgg cgcgccgata aactccaacc gtggaaaacg ggttaaatta
    10861 tgcagtggca taacagcctc cgatgtatgt tgttgtgatt ttcttattat gcacgctgaa
    10921 aacgcgtaaa taaaaaagg
//
LOCUS       DAQSWQ010000133        10832 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.135, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000133 DAQSWQ010000000
VERSION     DAQSWQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.135"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..277)
                     /locus_tag="R8G18_003661"
     CDS             complement(<1..277)
                     /locus_tag="R8G18_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9797261.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="R8G18_003662"
     CDS             complement(444..1184)
                     /locus_tag="R8G18_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HEE9797262.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="R8G18_003663"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="R8G18_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HEE9797263.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="R8G18_003664"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="R8G18_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HEE9797264.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="R8G18_003665"
     CDS             complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="R8G18_003665"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEE9797265.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="R8G18_003666"
     CDS             complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="R8G18_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEE9797266.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5609..6628)
                     /gene="epd"
                     /locus_tag="R8G18_003667"
     CDS             complement(5609..6628)
                     /gene="epd"
                     /locus_tag="R8G18_003667"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HEE9797267.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7000..7431
                     /locus_tag="R8G18_003668"
     CDS             7000..7431
                     /locus_tag="R8G18_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEE9797268.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7454..8032
                     /locus_tag="R8G18_003669"
     CDS             7454..8032
                     /locus_tag="R8G18_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEE9797269.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8033..8740
                     /locus_tag="R8G18_003670"
     CDS             8033..8740
                     /locus_tag="R8G18_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEE9797270.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8728..9405
                     /locus_tag="R8G18_003671"
     CDS             8728..9405
                     /locus_tag="R8G18_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEE9797271.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9399..10076
                     /locus_tag="R8G18_003672"
     CDS             9399..10076
                     /locus_tag="R8G18_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEE9797272.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10048..10761)
                     /locus_tag="R8G18_003673"
     CDS             complement(10048..10761)
                     /locus_tag="R8G18_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HEE9797273.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10758..>10832)
                     /locus_tag="R8G18_003674"
     CDS             complement(10758..>10832)
                     /locus_tag="R8G18_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEE9797274.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2580 a         2878 c         2699 g         2675 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaaa
     3061 gacccgcaga gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg
     3121 ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc
     3181 catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac
     3241 tgcccctgca gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc
     3301 cattgggtat cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt
     3361 tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg
     3421 ttgtgtttct tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg
     3481 ttacccggct tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg
     3541 ctgattttgc tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca
     3601 gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg
     3661 atttccagag tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg
     3721 ttctcttgca gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg
     3781 gtagctgcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc
     3841 ttcgcgcagt ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga
     3901 acgtgatgcg caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc
     3961 acgcctttac cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct
     4021 ttaactttag cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact
     4081 gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca
     4141 gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt
     4201 cgggccttat actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
     4261 gtcacttact tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa
     4321 ttatttagag tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc
     4381 ctgccctgtg attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac
     4441 tttaccttcc acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc
     4501 agcaatgccg aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc
     4561 ttcgctgtct gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc
     4621 gaacacgcca accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag
     4681 ttcctgagcg gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac
     4741 agatttcagg gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg
     4801 aaccgggatg ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta
     4861 cagggattta cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc
     4921 aacaatcagc tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac
     4981 tttagaacca ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag
     5041 cgcgtccagt tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat
     5101 accgtgagta gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc
     5161 acacagtgca gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa
     5221 gcgaacgttt tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc
     5281 tttaaccaga cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag
     5341 agagaattct tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac
     5401 tttcgcgcct tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc
     5461 gctggttact ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt
     5521 cccagcaaga tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc
     5581 taaaagtttt gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg
     5641 tcgtgtcgag cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga
     5701 tcaggtgtgc gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt
     5761 ggttaaaatc tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac
     5821 cttgtgctgc tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg
     5881 tcacgcttaa atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa
     5941 agcgatcgtt aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg
     6001 gaatgatcga ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa
     6061 cctgttgatc gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg
     6121 catcatctaa caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa
     6181 cgatgcggtg ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga
     6241 gatcgttact gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt
     6301 cgccatgctc gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt
     6361 cacgccaggg gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa
     6421 caaaaagttg atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat
     6481 atttcaacaa atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa
     6541 tttccgcccg gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga
     6601 agccatttat cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt
     6661 tgccagacag agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac
     6721 tgattggtta attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac
     6781 gtggaataaa aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt
     6841 ttcagccccg cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa
     6901 atcttttcgt tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc
     6961 gatattatcc gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca
     7021 tattcttccc ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc
     7081 cagctacacg cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc
     7141 ggtagcctga ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac
     7201 ggtacattcg aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat
     7261 ctctgcgttt ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc
     7321 gtgcggacca cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa
     7381 ccttgcgcgc tttctggtta cgacgagttg catatttccc ccgttaaata actactttca
     7441 aagaggtttt cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat
     7501 atctatcgcc atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt
     7561 tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct
     7621 gaccggctta ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt
     7681 tgcacccgtc gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt
     7741 tcgcaccctg ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt
     7801 cgctgtaccg ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt
     7861 cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacggtgat
     7921 aggcgcgaac ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca
     7981 gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc
     8041 atttacctca ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca
     8101 gaccattttg ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac
     8161 gcggcggcgg gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct
     8221 ggtacacggt ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg
     8281 gacgcatgct attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg
     8341 gatgcagtac gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc
     8401 aggcgtcgct tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt
     8461 agcgattatt catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca
     8521 gcgtttacgt gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct
     8581 ggctgtccgt ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac
     8641 gttgtgggca ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct
     8701 gatgctaaca gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg
     8761 acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc
     8821 gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg
     8881 tatccggact ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt
     8941 gggcatctcc cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat
     9001 ttgcagctct ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat
     9061 ctgtgtcttg ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag
     9121 tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg
     9181 gtcatcgcct gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt
     9241 cgcctgacgc cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa
     9301 cgtggttcgc tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag
     9361 caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat
     9421 cttatcgctg gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac
     9481 aaggtgaatg gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag
     9541 taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga
     9601 aaaacctgaa aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa
     9661 accaactttt tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat
     9721 gcccggcgga tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag
     9781 acactgccaa ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg
     9841 cctgcctgga agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg
     9901 acgaaaattg gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga
     9961 gcgtggtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc
    10021 gactggaaga tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt
    10081 catcattaag gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc
    10141 gtcggtacgg tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg
    10201 ccccaccaga cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca
    10261 aaattgcgca agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat
    10321 gacgagtggt gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta
    10381 ctgcggccac gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac
    10441 gtcaaatgtc tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt
    10501 gtaatggtga aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg
    10561 cacgagatat tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag
    10621 aaaaaccacc gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa
    10681 cggtttatgc acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt
    10741 caccgttaat tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc
    10801 agcgccgttt tgaccattcc gctaaaacgc gc
//
LOCUS       DAQSWQ010000134        10686 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.136, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000134 DAQSWQ010000000
VERSION     DAQSWQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.136"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..784)
                     /locus_tag="R8G18_003675"
     CDS             complement(<1..784)
                     /locus_tag="R8G18_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797275.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="R8G18_003676"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="R8G18_003676"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HEE9797276.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="R8G18_003677"
     CDS             1860..2747
                     /locus_tag="R8G18_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEE9797277.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="R8G18_003678"
     CDS             2780..3790
                     /locus_tag="R8G18_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEE9797278.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="R8G18_003679"
     CDS             3860..5293
                     /locus_tag="R8G18_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9797279.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="R8G18_003680"
     CDS             5392..6741
                     /locus_tag="R8G18_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HEE9797280.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="R8G18_003681"
     CDS             6731..7618
                     /locus_tag="R8G18_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEE9797281.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="R8G18_003682"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="R8G18_003682"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HEE9797282.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="R8G18_003683"
     CDS             8352..9224
                     /locus_tag="R8G18_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HEE9797283.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="R8G18_003684"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="R8G18_003684"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEE9797284.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="R8G18_003685"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="R8G18_003685"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HEE9797285.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2530 c         2855 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAQSWQ010000135        10664 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.137, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000135 DAQSWQ010000000
VERSION     DAQSWQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.137"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(270..1043)
                     /locus_tag="R8G18_003686"
     CDS             complement(270..1043)
                     /locus_tag="R8G18_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEE9797286.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="R8G18_003687"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="R8G18_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HEE9797287.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="R8G18_003688"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="R8G18_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HEE9797288.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /locus_tag="R8G18_003689"
     CDS             1926..2666
                     /locus_tag="R8G18_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEE9797289.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="R8G18_003690"
     CDS             complement(2637..3404)
                     /locus_tag="R8G18_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEE9797290.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="R8G18_003691"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="R8G18_003691"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEE9797291.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="R8G18_003692"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="R8G18_003692"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HEE9797292.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="R8G18_003693"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="R8G18_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HEE9797293.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="R8G18_003694"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="R8G18_003694"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HEE9797294.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..9490)
                     /locus_tag="R8G18_003695"
     CDS             complement(8354..9490)
                     /locus_tag="R8G18_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEE9797295.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(9959..10336)
                     /locus_tag="R8G18_003696"
     CDS             complement(9959..10336)
                     /locus_tag="R8G18_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797296.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            complement(10336..10614)
                     /locus_tag="R8G18_003697"
     CDS             complement(10336..10614)
                     /locus_tag="R8G18_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HEE9797297.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
BASE COUNT         2665 a         2624 c         2566 g         2809 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca gtgcagctta ttctccacgt
     8581 gccagtggtt tcggattgct gtggcgaact tctctgcggt taaatcagca gaactgatat
     8641 aatatctgac cgtcatttct ggctctttct tttgttctgc tattatggac cgaaaggaga
     8701 ctgccacgca taatttcttc agccctttcc attcaaacgt gaaatcaata agttcatcag
     8761 ggacatcgca aacaatatga agacggattt cttctctgcc gtgactcttt tcactgattg
     8821 cgtaactgtc atgctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt
     8881 tattaagccg cccctggttt ccttttaccg cgaataaata atcaccgccc tgtttttgta
     8941 tcttctctgc aatatctttc tggcatccca tcgcatcagt tgtgatgatt tttcctttaa
     9001 tatccagcat gttaagaagt tcaggaatgg ctgtaatctc attggatttc tcatccgtct
     9061 taatctgccc gagaaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc
     9121 ccttgcggcg actcttgtca taagagtgcc ggagcgtttt tccatcaatt gcaatgacgt
     9181 ctttatcatc tgaagaatgg cagtcacgca tccagttaat aaagcactcg tgaaattttg
     9241 caggactgat acaggataca actctggcaa tagtatcgtg aacaggaata ccattttcaa
     9301 aatcaccata ttgcttcaaa aaatcgagat gtgtttcacc aaaatcctct atatcttccc
     9361 aaccctctgc accagaaatg acggcacaaa tagtcaacag tagaatatct gacaatttat
     9421 gttctacttt ccaggcttgt ctgtaatcgg ggataataga aatatgttcc atcagttttt
     9481 taagttccat tttgttctcc ttatttatgc aaggagtatt tgatcatata taagctatga
     9541 aaaatagttt taggtaataa ggaatatctc aattattgaa catttagcgc gaattattta
     9601 gtacaaaaag tgtacttccg atgatctttc cctgctccaa agtcttctaa aatcaacaaa
     9661 gtagactctt aattatattt atcaacacaa tagtctatcc tattactatc ttgacaagag
     9721 agaaccgttt tttatttaaa tatatgatac acatcattaa atatataacc atactttttc
     9781 ataaggcaat ctaacttagc tcttaaatca ccaaagccca tcacaccata acatctaata
     9841 gccattgcca cacgtcacta tggatttatt tccacctgca attacttcgt cctaacttta
     9901 atttaatctc aagaagcatt aagatctcaa tagcgagctt aatacttctt gaataatatc
     9961 actcctgacc aaaatttttc cacactaatg catgagactt tgatttgtca cccagaaatt
    10021 tacttaaatc ttgccaaagt aaagaattgc tatcggtcgc agtgactatt gcctctataa
    10081 gccctgtagc tacaactgtg gccaattcat catcaagcga gaccatacca tcttcgacat
    10141 gtttaaatat tttttctctt tcaatatccc ccatttcata aaaatttttt acaatcgttt
    10201 ttccaacctg agagaacaac ataaccaccg gtggcgtttc tggatgccag tattcattag
    10261 ctaatgagac tgtctcagac aaaacagtgg aaaatgatgc cattttttct atataactag
    10321 caatatattg gttcattact tgcctcccaa agcattctgt aaatcctgaa gaacgttttc
    10381 tgctgctgct cgatcccctg gagatgctgt tggattctta tccaaccaat cctgtaaagc
    10441 ccttgtataa tcctgtgctt tcaatacatg tcctctacca ccaacttttt cacctgtggc
    10501 caattcataa cgaacagcat ctgctgtgct tccacttcct acttttgcat ttggccgata
    10561 aagatcgtct ataatatttt tcaaatttga atctgtaacg tcgggttttc ccattttctg
    10621 tcgcaattca tctttatacc gttcatgctg catacgatcc cttg
//
LOCUS       DAQSWQ010000136        10536 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.138, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000136 DAQSWQ010000000
VERSION     DAQSWQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.138"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(71..1099)
                     /gene="malI"
                     /locus_tag="R8G18_003698"
     CDS             complement(71..1099)
                     /gene="malI"
                     /locus_tag="R8G18_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HEE9797298.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1274..2866
                     /gene="malX"
                     /locus_tag="R8G18_003699"
     CDS             1274..2866
                     /gene="malX"
                     /locus_tag="R8G18_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HEE9797299.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2876..4048
                     /gene="malY"
                     /locus_tag="R8G18_003700"
     CDS             2876..4048
                     /gene="malY"
                     /locus_tag="R8G18_003700"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HEE9797300.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="R8G18_003701"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="R8G18_003701"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEE9797301.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5264..6229)
                     /locus_tag="R8G18_003702"
     CDS             complement(5264..6229)
                     /locus_tag="R8G18_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEE9797302.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPAS"
     gene            6473..6598
                     /gene="blr"
                     /locus_tag="R8G18_003703"
     CDS             6473..6598
                     /gene="blr"
                     /locus_tag="R8G18_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HEE9797303.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6863..7078
                     /gene="ydgT"
                     /locus_tag="R8G18_003704"
     CDS             6863..7078
                     /gene="ydgT"
                     /locus_tag="R8G18_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HEE9797304.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7164..7604
                     /locus_tag="R8G18_003705"
     CDS             7164..7604
                     /locus_tag="R8G18_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HEE9797305.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7681..8262
                     /gene="rsxA"
                     /locus_tag="R8G18_003706"
     CDS             7681..8262
                     /gene="rsxA"
                     /locus_tag="R8G18_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEE9797306.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8262..8840
                     /gene="rsxB"
                     /locus_tag="R8G18_003707"
     CDS             8262..8840
                     /gene="rsxB"
                     /locus_tag="R8G18_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEE9797307.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8833..>10536
                     /gene="rsxC"
                     /locus_tag="R8G18_003708"
     CDS             8833..>10536
                     /gene="rsxC"
                     /locus_tag="R8G18_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEE9797308.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KA"
BASE COUNT         2408 a         2724 c         2696 g         2708 t
ORIGIN      
        1 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
       61 aaagagagga ttatttcgct gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
      121 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
      181 caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc gcatcggtaa
      241 atgccgccag cgaaacttgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
      301 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
      361 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
      421 ggctggatgt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
      481 ttgcacaata gcctcccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
      541 aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
      601 tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
      661 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
      721 caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct
      781 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
      841 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
      901 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
      961 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     1021 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     1081 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     1141 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcttcact
     1201 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
     1261 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     1321 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     1381 cggcattggt agttctctta gtagccacga tgtcatcacc ctgatcccgg tcctgggcaa
     1441 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     1501 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     1561 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     1621 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     1681 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     1741 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgcttgcatt
     1801 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggcc ttgtcggcct
     1861 ggtgattcca ttagtctggc cgattttcgc gatgggtatt agcggcttgg gccatatgat
     1921 aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     1981 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     2041 ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc aattgagttg
     2101 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     2161 tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg cgcgcccgga
     2221 aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     2281 cacgaccgaa ccgctggaat tcctgttcct gttcgtggcg ccagttctgt atgtcatcca
     2341 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     2401 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     2461 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
     2521 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag tggccagttc
     2581 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     2641 cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta cccgtctgcg
     2701 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     2761 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     2821 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     2881 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc
     2941 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     3001 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag
     3061 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     3121 caccgcaatt gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga
     3181 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga
     3241 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     3301 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga
     3361 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     3421 tgagctggag atcatggcag acctgtgcga gcgtcatgat gtgcgggtta tttccgatga
     3481 aatccatatg gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     3541 gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg
     3601 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
     3661 acgtgatggg ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca
     3721 gcaaggtgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     3781 ctcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta
     3841 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     3901 tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg
     3961 ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     4021 aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg
     4681 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
     5161 ggatggtgca atttgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg
     5221 cggattcgat gcccagctca atcaactcca ttacctggat tgcctagctt gccggaaccg
     5281 gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc
     5341 caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
     5401 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
     5461 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
     5521 gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg
     5581 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
     5641 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta
     5701 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
     5761 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
     5821 gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca
     5881 cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa
     5941 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
     6001 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
     6061 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc
     6121 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
     6181 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
     6241 tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac
     6301 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aaatagcgaa ttttccaacg
     6361 acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag
     6421 taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg
     6481 tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc
     6541 gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc
     6601 tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt
     6661 aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc
     6721 gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta
     6781 ttgtaataac acaagcgttc acttttggtg aaattttatc atcactctac ccctttggta
     6841 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     6901 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     6961 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7021 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7081 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7141 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7201 tgcttggccc gttggcttgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7261 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7321 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     7381 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     7441 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
     7501 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     7561 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     7621 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     7681 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     7741 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     7801 atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat
     7861 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     7921 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     7981 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8041 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8101 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8161 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8221 tctctggcct ttatgggctt taatggtttg gtgaagttgt aatgaatgct atctggattg
     8281 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     8341 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     8401 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     8461 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     8521 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     8581 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     8641 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
     8701 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     8761 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     8821 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     8881 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
     8941 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9001 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9061 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9121 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9181 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
     9241 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
     9301 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
     9361 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
     9421 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
     9481 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
     9541 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
     9601 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
     9661 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
     9721 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
     9781 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
     9841 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
     9901 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
     9961 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    10021 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    10081 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    10141 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10201 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    10261 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10321 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    10381 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    10441 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    10501 actgccgttg aagcagctat cgcccgcgcc aaagcg
//
LOCUS       DAQSWQ010000137        10483 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.139, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000137 DAQSWQ010000000
VERSION     DAQSWQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.139"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(164..2098)
                     /gene="yeaG"
                     /locus_tag="R8G18_003709"
     CDS             complement(164..2098)
                     /gene="yeaG"
                     /locus_tag="R8G18_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HEE9797309.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            2178..4111
                     /locus_tag="R8G18_003710"
                     /pseudo
     CDS             2178..4111
                     /locus_tag="R8G18_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4434..4808
                     /locus_tag="R8G18_003711"
     CDS             4434..4808
                     /locus_tag="R8G18_003711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797310.1"
                     /translation="MRKKENKAKKEKFRQFFPENYDIGISLNEENQLRLFSKRNDSQD
                     ESLCKYEFSNKIKVQYIFLPYSSEIQVITDEFPLTEAGCQECTQAFKHPISMELIEEI
                     KEAKCSVTGLVIYSKPILKLIS"
     gene            5247..5432
                     /locus_tag="R8G18_003712"
     CDS             5247..5432
                     /locus_tag="R8G18_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797311.1"
                     /translation="MKKVTLKERKHHVVTARLNDDQVLVLNQLISSGKVQKDSLSACV
                     QYLINQYMILQGGGNEE"
     gene            5422..5724
                     /locus_tag="R8G18_003713"
     CDS             5422..5724
                     /locus_tag="R8G18_003713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797312.1"
                     /translation="MKNDFDLVQYFDDYTIVGDMDYHYFYCVKYQEGSLEAHYERIGD
                     PEVLGTITWNGNYLIDGLPMVMFVLAAEDEHKECLNRLLIVCEKARDEYRLNATGL"
     gene            5779..6333
                     /locus_tag="R8G18_003714"
     CDS             5779..6333
                     /locus_tag="R8G18_003714"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEE9797313.1"
                     /translation="MNPKITWRLIPNSKHYYVSENGDVYNDKTKQYIATKNPSNGYKK
                     VSINGKSLYVHVLVCTLFNGAKPIGGRYTVDHIDRDKMNNKAKNLRWVTQKVNNSNRK
                     HKGTRTQKLTLKQWLMIVGEYLKGQLSQYAITKWFLNQTGINICPQNIGKLFKGTTYS
                     KWYTTYLTNKQRQRIQSLTIQRKP"
     gene            6455..6916
                     /locus_tag="R8G18_003715"
     CDS             6455..6916
                     /locus_tag="R8G18_003715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797314.1"
                     /translation="MAKTYSTFDNRNIKALISQYKLYKDIPCEDIKNIMKDSVAKLKV
                     RDKISVYDVFAVLSNEDELRNDLLFNTINAIKVQQNKKQVKERMIRYYGAAITDAAKR
                     IEQYMLAHPNALPAPVYGQRTLTYKEFGKLLELQKQGDFIDDMIEYLKGLR"
     gene            7380..8123
                     /locus_tag="R8G18_003716"
     CDS             7380..8123
                     /locus_tag="R8G18_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEE9797315.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            8206..8679
                     /locus_tag="R8G18_003717"
     CDS             8206..8679
                     /locus_tag="R8G18_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797316.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFELVSSDLVTIDNECDICHLEDITILDVEGNEHKIPNSHRLIALLKQDKKT"
     gene            complement(8936..9604)
                     /locus_tag="R8G18_003718"
     CDS             complement(8936..9604)
                     /locus_tag="R8G18_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797317.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNNTEPDIEPKNFAHLVKTEREVIDLLNIKLASLKSELQSLTFWKVSPKDEAIFNNY
                     FFSNQIALGTLREAISFDINDIHDRYHYNVSYGKLLVDTFDSLIEAHATLKKDMNNLF
                     VFSN"
     gene            complement(9737..10048)
                     /locus_tag="R8G18_003719"
     CDS             complement(9737..10048)
                     /locus_tag="R8G18_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797318.1"
                     /translation="MSKDGYDPSITRGVQKFSHNGRWATVCYWGSALTEFHYGDRVFF
                     QNQYREYWLGTIERDCFVLTCETPLISVIDGLAYLSAEYRMNQSSEEGDWFCDQGELP
                     F"
BASE COUNT         3297 a         1961 c         2216 g         3009 t
ORIGIN      
        1 caccatgctt ttgtttttgc cgttcagacg ccggtcaata aacccggtca tatgcccccc
       61 gtactacatt tgccaactgt atgctacgcc tgtgttgcca tttctctggc ccacggttcg
      121 ctgtcgttat aggcccggta gcatgtgtac cgggccaggg ttgttaagac gatttacgta
      181 cgcgcaaata ccattcgcac agtaaacgca cctgtttacg ggtgtagcct ttttccatca
      241 tacggtcgac aaagtcgtcg tgtttcttct gctcgtcggt tgacgttttg gcgttaaacg
      301 agataaccgg caacagctcc tcggtattgg agaacatttt cttctcgata accgtgcgca
      361 gtttttcata gctggtccag ttcggattgc gtccgctgtt attcgctctg gcgcgcagta
      421 cgaagttaac aatctcgttg cggaaatctt ttggattact gatccccgcc ggtttctcga
      481 ttttctccag ctcggcgtta agagactcgc ggtcaaacag ctgcccggta tccggatcgc
      541 gatactcctg atcctgaatc cagaaatccg cgtaggtaac ataacggtcg aaaatgtttt
      601 gcccatattc ggaataggat tcaaggtagg ccgtctggat ctctttgccg ataaactcgg
      661 catatttcgg gatcagataa cctttcagga actccagata gcgttctgcc tgctcttgcg
      721 ggaactgctc gcgctcgatc tgctgttcta ggacgtagaa cagatggacc gggtttgccg
      781 ccacttctac atgatcgaag ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg
      841 acagaccttt catcccttca tcgacaccgg cgtagtcacg atattcctga tacgacttgg
      901 ctttgggatc ggtgtctttc agactttcgc catcataaac ccgcatcttt gaataaatgc
      961 tggagttttc tggctctttc aggcgcgaaa gaatggaaaa acgtgacagt gtttccagcg
     1021 tgccaggggc gcatggggcg tgagtcaatt cactgtgatt aagcaatttc tcgtagattt
     1081 tgatctcttc ggaaatgcgt aagcaatacg gcaccttcac gatgtaaaca cggtcgagga
     1141 aggcttcgtt gtttttgtta ttacggaaag tgacccattc ggattcgttc gagtgggcga
     1201 gaataatccc gttgaacggc agggcggaga taccttccgt cccgttgtag ttaccttcct
     1261 gagtagcggt taacagcgga tgcagcactt taatcggtgc tttaaacatc tcgacaaatt
     1321 ccatgatccc ctgattggcg cggcacagcg caccggaata gccgtaggcg tccgggtcat
     1381 tctgcgcgta gtgttcgagt ttacgaatat cgactttccc taccagcgcg gagatgtcct
     1441 ggttgttctc atcaccgggt tctgttttgg cgatagcaat ttgttgcaga attgacggcc
     1501 agaccttcac tacccggaac ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg
     1561 cccacggcga catgatggtg ccgagatagc ggcgaggaat gccatactct ttttccagaa
     1621 tctgcgcatc ttcctgcgga ttgaaaagac agaacggatg atcgttgacc gggctacgct
     1681 caccgttggc gctcaataca taaatcggta cgagctgcat taatgatttc agtcgctcag
     1741 caagcgatga tttaccccca cccacaggcc ccagcagata caggatttgt ttcttctctt
     1801 ccagcccctg agccgcgtgt ttcagataag agacaatctg ttcaatcgcg tcttccatgc
     1861 cgtaaaactc ttcaaatgcc ggataacgtg caatgacccg gttagaaaag agtcgagaaa
     1921 gtctgggttc ctgggctgta tcgaccatga caggctcacc gatagccatc aatagccgct
     1981 cagccgcgtt ggcataagca ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg
     2041 tgaactcttc gtccttggca gcttcatatc gctggcgata gtgatcgaat atattcatgg
     2101 tatgccgtcc ttttgtatac tctgggaaca tttgtgaatc agcactttta gcgtaattct
     2161 tcaacgggat aaaaaatatg ctccgtccta tttgttatga gcgagtatca agcattcagc
     2221 aagttgatat gggtaaaggt ttggacgacc aacgatcatc attgactgca tacttagata
     2281 aaaatgctga taagttcact gacgaaagga ttttcattac tgatgcgggg ctttccgcat
     2341 tcaaaaatgc caatatctct ccagagtctc agcttgggat atttttgcaa gatgtgggca
     2401 accgcaagta cggcgtaggt gacgcacttg tggttttatc gctggatcgt ctatcgcgtc
     2461 gatcaagctg ggccgaaagt actatccagt acatcgtgaa tagtgggatt gagattcacg
     2521 atatctctac aaaccttgtg ttacgggttg atgatcctat gtcaaaaatt cacatggaac
     2581 tgatacagca ccgttcacac aatgagtcat tgatgaaatc ggtacgtgca aaattggcat
     2641 gggataacaa aatcacacgt gcagtacaga acggggaagt aatctccaac cgtatgccta
     2701 attggttgag aaatgtagat aacaagtacc aagttatacc ggaacaggca gaactgattg
     2761 ttaaatgttt catgtggtat agggaagggt tgagtactgg cgagattgtt aaacgtatcg
     2821 gtagaaagtg gcagatggtc actgtgtcca ggctgatacg tgatcgccgt ttgttgggcg
     2881 agcatgaacg ttacaacggt gaggtagtac caaacgttta cccgaaaatc atcgacgaag
     2941 agttgttcct gacagcgaat agaatgatgg atcgcgttat gttggagaag aagaagccag
     3001 cagaggatct attacttgaa ccagaagttg tccgagagat ttttaaactt tatgagacag
     3061 gccttggttc aggggcgatt gtaaaacgct tgccgaaagg gtggagtacc gtcaatgtac
     3121 tgagggtact acgtgataag aaagtagtta ccatgaagat tatcgataac ctcacctttg
     3181 aacgggctaa tgagaagtta tcgcggaatg gtatagctaa tcgtatccgt aaggacatta
     3241 ccattgctca ggatgattac attactaacc tcttcccgaa gatgctgaaa tgcggtcact
     3301 gtgggggcaa tattgcaatt cactataatc atgtacgaac taagtatgtt atttgtcgta
     3361 acagggaaga aaagaagatc tgcgatgcta aatctatcca gtacatgaga attgaaaaga
     3421 acattctaca gcttgtcaaa aatgtagact tcaataaatt gatgatggaa ggtaagtcgg
     3481 attcagattc attgcttgca tcattgcgag aagagttaag tactttacag aaagaggcag
     3541 aaacctatca ggttaaaatt aatgaacgta aggcagcagg taagaaagcc tcgttaccat
     3601 taatgacggg tttaactgag gtacaggata gaattgaaga aatagaactg agatattaaa
     3661 ttcaaagaac atcaaagaaa tccctgtatt tgatttcgat attaataaag tacttgatcc
     3721 attgaatgtc gagttacgtg caaagttacg gaaagaagta agattagtac tgaaatctgt
     3781 taagtactgg gtttttgaaa agcgtatatt tgtacagttg gaatacttca atgatgtcct
     3841 atcccatacg cttgtgattg ataacaagcg gggcgggggt gaagtactac atgagatcgt
     3901 aatctctgaa acaagcggcg agcgtgtcta cactgtcaat gaagagggca aaaccgtttt
     3961 cattgcctcc gttacatctg gtactgatat gtggtcgctt gccattagcc gtaccaaaat
     4021 acttgatgat gttggattgt atcttaatct tctcaagcgt gaaaaatttg agatcattgt
     4081 taatgaagat atgattgatt ggatggatta aaagctaatg agaatttatg gtcttgtaga
     4141 atgaattatg cgagttaata tttgaacaat atatctacga tgtcgaaatc taacggcatt
     4201 gtagatagag caataaaaga gggattatga attttggata taactacgca cccgatgggt
     4261 ttaagatttc cgtgcaaaat acagcaaatc atttatatca gcgtgtgtta gaatagtcaa
     4321 gggtaaaatt aacttttttg acttttcaac gttaaaccag taccatagcg tcattcacta
     4381 acgaatagga gcgatgaaac catgagatga attctaaaga gaggattgcc aaaatgcgta
     4441 agaaagaaaa taaagcaaaa aaagaaaaat tccgtcaatt tttccctgag aattatgata
     4501 ttgggatctc attgaatgaa gagaatcaat taaggctttt ttcaaagcgg aatgattctc
     4561 aagatgagag cttatgcaag tacgaatttt ccaacaaaat taaagttcag tacatttttt
     4621 tgccgtattc ttctgaaatt caagttataa cagatgagtt tccgctaacg gaggcaggct
     4681 gtcaggagtg tactcaagct ttcaaacatc caatatcaat ggaactaatt gaagagataa
     4741 aagaagcaaa atgttcagtt actggacttg tcatttattc gaaacctata ctaaaactta
     4801 ttagctaaca catccaaatt gttacgtttt aagtactccc tcactaaata ctataaagtt
     4861 gaatgtgaat gggggtactt caaaatggaa tggtttacta aattacttta cgagatagta
     4921 ggctttgtac tttacatctt tctgataatc ccaatatata tagtgctcat gtttttatct
     4981 atgattttag tactattgct ttccccatta aaaatcaaat cgataaataa atacgtagta
     5041 agaaaaatga aacgctaaaa gttttggtgc tatactaata gtatcagaac gataatgcgt
     5101 tttgccttcc gctccagctt tgaacataaa taatatagcg acaataaatt tcctttttaa
     5161 ttaagattgc ctctcttgcg agagaggtat ctttttaatt tggatttata aatacagtat
     5221 acaaatacac ctataaggat atagaaatga aaaaagtaac tttaaaggaa cgcaaacatc
     5281 atgtagtaac cgcacgtctt aatgacgatc aagtacttgt acttaaccag ctaattagta
     5341 gtgggaaggt acagaaagat tcattatcag cctgtgttca gtacttaatt aatcagtaca
     5401 tgattttaca gggcggtggt aatgaagaat gactttgatt tagtacaata ttttgatgat
     5461 tacacaatag taggggatat ggattatcac tatttctatt gtgtgaagta tcaggaaggt
     5521 tcattagaag cacattatga acgaattggt gatccagaag tattaggtac tattacatgg
     5581 aatggaaatt acctaatcga cggattacca atggtaatgt ttgtactggc tgctgaagat
     5641 gaacataagg aatgtttaaa ccgtttgcta atcgtatgtg agaaggcgag ggatgaatat
     5701 aggctaaatg ctactggcct ctaagtagta gcagacgcga tcacatggag tgacgcgata
     5761 acaacaagga cgtttgatat gaaccctaaa atcacttgga gattaattcc aaattcaaaa
     5821 cattactacg tatctgaaaa tggcgatgtt tacaatgata aaacaaaaca gtatatagct
     5881 acgaaaaacc catctaatgg atacaaaaaa gtttctatta atggcaaatc actatatgta
     5941 catgttttgg tttgtacttt attcaatggt gctaaaccca ttggaggaag atacactgta
     6001 gatcatatcg accgcgataa aatgaataac aaagcaaaaa atttacgttg ggtaactcag
     6061 aaggttaata attccaatag aaagcataaa ggtactcgta ctcaaaagct tactctaaag
     6121 caatggttaa tgattgttgg agagtacctt aaaggacaat tatctcaata tgcaatcact
     6181 aaatggtttt taaatcaaac tggtattaat atttgccctc agaatatagg taagttattc
     6241 aaaggtacta cctattcaaa atggtacaca acatatctta cgaataagca aagacaaaga
     6301 atacaaagcc taaccattca acgtaaacca taaaaaatgc cagtattcca gtactggcat
     6361 ctattcaaaa ctcaatggca atgaagccaa acttattaaa tggcctgtaa tcactacggg
     6421 tcacgattaa ttaatttata caggattatt tatcatggca aaaacctatt caacttttga
     6481 taacagaaac ataaaagcac taattagcca gtacaagcta tataaagata tcccatgcga
     6541 agacatcaag aacatcatga aggactcagt agctaaactt aaagtgagag ataagattag
     6601 tgtatatgat gtgtttgcag tattgagtaa tgaagatgaa ttacggaatg atttactatt
     6661 caatactatc aatgctatta aagtacaaca gaataagaaa caagtaaaag aacgtatgat
     6721 caggtactat ggggcagcga tcacggatgc agcaaaacga atagagcagt atatgttggc
     6781 acacccaaac gcactgccag caccagtata tggacaaaga acactaacat ataaagagtt
     6841 tggtaaacta ttggaactac aaaaacaagg tgactttatt gatgacatga tcgagtactt
     6901 aaagggcttg cgttaattgt aacttattac ggcatattac ggcatggatg tatgccgtaa
     6961 tacggcaaaa aaagcccctg aaacccgcta tttaccatca atacggcaac taagtataga
     7021 gatacgaaat ccattccatt tcattccatg tatttctttc tctatcagta ttccaagaaa
     7081 ctgtgacttg cgtcacgaat actactcgtc gtgaactcct cgtagtattg gttagtaacg
     7141 ttgcgttttt ctcgccagta ctcgaaaacc cgcaaggcgt tactactcgc tttgctcgat
     7201 agatgttagt acagcaaatg aagtacctaa agtgaattgg taagtacgac atgccccgcc
     7261 agttccacaa ctctcgctct ggtcatttcc actactatga tgctattgga tagctacata
     7321 aagtactgga tcaagtaggc attttttgtg tacaaagagg gaattaagag agggatacca
     7381 tgaacagaaa acaattcatc cagtcgcatg gtgctacttg tagtaactgg acttggagtt
     7441 ggtcatttgt gaaccacgac aagaaaatgg ttatctttgg tgcatgggat gtacaaaaag
     7501 agcaagaaag atcagtcatt ctgagggagc ggtggaaaac caaagttgtg gagggaaagg
     7561 aaagaaaaaa tcttggatat attcaagcta ttgaacacat tcaattgata gctgaaggtt
     7621 atgagcttta cacgtttgat atggagcagg gacgaaacga tgagaataga gatgtagctg
     7681 ttatcaaacg gttcactcca aaacttgaaa aacgttacct tcggaaagaa ggaacagttt
     7741 ggtatgcaga ctttttacct aatccatttc cagatgaaat tacctcacct gaaaactacg
     7801 ctgagggtgc aaaaaagcaa gttacagtaa actcctacga gcgtgatcca aaagcacgac
     7861 aggcttgtat tgaccatcat ggtacttcct gcgggtgttg tgagtttaac tttgagaaag
     7921 tctatggtga acacgccaag ggctttatac acgttcatca tatcaaaccg ctacacacag
     7981 taggcgagga ttacgaggta aatccaatta ctgatatggt tccactttgt ccaaactgtc
     8041 acgcaatggt acatcgcggt aacgaagtat taagtgtcga aagtttaaaa gcactgttgt
     8101 ttaaagacag caataaaatt tgaatttatt ttagacttga tacgacatac ttgaaaagtt
     8161 atgaatacaa tatgctttaa attagacatg taagttgaga gaaaaatgag cataacaaca
     8221 atagcagcta catataaaga tgtgttaact attgcttttt cggcgttgtc atttattatt
     8281 gcatgtactt ccttatactt ctccggtagg gcagcatggc atgatagatc aaggctaaaa
     8341 ataacagctg acctcgtcta cgagtctttt gatgaggaac cttataaaat cgaggtgata
     8401 gtgatcaatg ttggtaggcg tgatgcagtt ttggaaggga tgctttgcca ttatgaaaaa
     8461 ggcattagga gccattcatc tccaaaagag agcattgtta ttaaagaaaa gcaaagaaaa
     8521 aaatttgaat tggtaagctc agacttagtc acgatagata atgaatgtga tatttgtcat
     8581 ttagaagata taactatttt agatgtggag gggaatgaac ataaaatccc taactctcac
     8641 agactcattg cattacttaa gcaagataaa aagacttaag aataatattt tttggaatgt
     8701 aaggtaaatg ttagatgcca ctatagattg agggttagaa ctactaaccc actatagggt
     8761 ttacgagatg ataagaatgg aatttaacac ctacgatggt gatatatatt acttcctcta
     8821 tttatatagg aggtacgaaa gggggagtac tttaatgaag accttataaa tcttaatgtt
     8881 gagatgttag ttatagcggt gaggataccc caccgtctat tgggagttgt ggactctaat
     8941 tagagaaaac gaataagtta ttcatgtcct ttttcaaggt tgcatgtgct tctattaaac
     9001 tatcaaatgt atctactaat aactttccat agctgacatt gtaatgatat ctatcatgaa
     9061 tgtcatttat atcaaaactt atcgcttccc tcaatgttcc taaagcaatt tgattactga
     9121 agaaataatt attgaatata gcttcatctt ttggcgatac tttccaaaag gtaagtgatt
     9181 gcaattcgct ttttaatgag gctaacttaa tgtttaatag atcgataact tctcgttcag
     9241 ttttaactaa atgtgcgaaa ttttttggtt ctatatctgg ttcagtattg ttaaggttgc
     9301 atatattttc atatgttttc aacatctcca acgaaaaaag atcgcaatta gtccagaact
     9361 tagtaacttg atttaaagca ttttccttta ttttagtttg aaaccaattt ttagcgttcc
     9421 atgctgcata tagggctgct ccagccataa tgagattggc aatagaacta acccagtcac
     9481 taaccgatcc ccactcgaaa ccttcagaac caacaaagag tgattttatt agtactaaaa
     9541 ttacaacaac cagtaatact gctacggtcg ataaaagaac gatatttaat tttctacaat
     9601 ccatcgaaag tagttcctga acgcattaaa ggggcaaata ctttaccatc cgaaacacaa
     9661 tcaagtacac tacccgttca cgtactgctc acatgttggg tttgttattt tgttggggtg
     9721 agcagtacgt gtatcctcag aagggtagtt ccccctgatc acagaaccaa tcaccttctt
     9781 cactgctttg attcatccgg tactcagcag atagatatgc taacccatca ataacactta
     9841 ttagtggtgt ttcacaggta agtacaaaac aatctctctc tatagtacct aaccagtatt
     9901 ccctgtactg attctggaag aataccctat ccccataatg aaattctgtt agtgcagatc
     9961 cccaataaca cacggttgcc cagcgaccgt tatgtgagaa cttctgaacc cctcgcgtaa
    10021 tggatggatc atagccgtct ttgctcattt gttaccctcc tctatgtatt actgtatata
    10081 tgtacagtat tgcagtcagt ttcccaaaaa tccagcaccc ctttgtttac cactttgaat
    10141 gattaattct attgtaatga tcaataaaat tgatcgattt aattgccaat gttttaaaaa
    10201 tgatcaatta tcaataactt aaagcgtaac gagcaaagtt atgaacattg ataattgggc
    10261 aataccgata acaagtttgg gtggtcagtt gctgggggtt gagttggtca gccatgtaga
    10321 acgcgaggga atcaggcaga tcggcctatg ggaagaagaa gtacttcatg ggcaactaat
    10381 gcttattgaa agcaaggcgg catggttcag ggataacggt ttgtattgtg tgattactac
    10441 tggccgtggt gaaccatacg agtacttacc attccttcgg cag
//
LOCUS       DAQSWQ010000138        10455 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.140, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000138 DAQSWQ010000000
VERSION     DAQSWQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.140"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="R8G18_003720"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="R8G18_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HEE9797319.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="R8G18_003721"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="R8G18_003721"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEE9797320.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="R8G18_003722"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="R8G18_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HEE9797321.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="R8G18_003723"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="R8G18_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEE9797322.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="R8G18_003724"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="R8G18_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="R8G18_003725"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="R8G18_003725"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEE9797323.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="R8G18_003726"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="R8G18_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HEE9797324.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="R8G18_003727"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="R8G18_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HEE9797325.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="R8G18_003728"
     CDS             complement(9457..10149)
                     /locus_tag="R8G18_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEE9797326.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2557 a         2521 c         2763 g         2614 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggattttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acacc
//
LOCUS       DAQSWQ010000139        10369 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.141, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000139 DAQSWQ010000000
VERSION     DAQSWQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.141"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="R8G18_003729"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="R8G18_003729"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HEE9797327.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="R8G18_003730"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="R8G18_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HEE9797328.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="R8G18_003731"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="R8G18_003731"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEE9797329.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..4843
                     /gene="uidB"
                     /locus_tag="R8G18_003732"
     CDS             3749..4843
                     /gene="uidB"
                     /locus_tag="R8G18_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HEE9797330.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTY"
     gene            4844..5545
                     /locus_tag="R8G18_003733"
                     /pseudo
     CDS             4844..5545
                     /locus_tag="R8G18_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(5726..7234)
                     /locus_tag="R8G18_003734"
     CDS             complement(5726..7234)
                     /locus_tag="R8G18_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEE9797331.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7335..8510)
                     /gene="manA"
                     /locus_tag="R8G18_003735"
     CDS             complement(7335..8510)
                     /gene="manA"
                     /locus_tag="R8G18_003735"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEE9797332.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8709..10355
                     /gene="fumB"
                     /locus_tag="R8G18_003736"
     CDS             8709..10355
                     /gene="fumB"
                     /locus_tag="R8G18_003736"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HEE9797333.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2549 a         2482 c         2686 g         2652 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat taacatcgcg cacgttcttt
     4861 tttagccgcc agttggcatg atgataccag tcgctattct ctgggggcat cggtacagaa
     4921 agatgtttcc aatcagatac aaagtattct cgagaaaagc ataccgctcg acccgaatta
     4981 tacgttgaaa ggggagttgc tcggctttta cgcgcagctc gaaggtttaa gtcgtaatac
     5041 cagccagccc aatgaaacgg cgttggttag tggacaattg acctggaatg cgccgtgggg
     5101 aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg aatggtgccg tggtggatac
     5161 cgacattggc tatccctttt cattaagtct tgatcgtaac cgtgaaggaa tgcagtcctg
     5221 gcaattgggc gtcaactatc gtttaacgcc gcaatttaca ctgacatttg cacagattgt
     5281 gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt gaaggcgcgg gtatcttagg
     5341 tggtatgaac tatcgggtca gcgaagggcc gttacaaggg atgaatttct ttcttgctgc
     5401 cgataaaggg cgggaaaagc gcgatggcag tacgctgggc gatcgcctga attactggga
     5461 tgtgaaaatg agtattcagt atgactttat gctgaagtca gaaataacgc cggagagaaa
     5521 aacctccggc gtttcagatt gttgacaaag tgcgcgtttt ttatgccgga tgcggcgtaa
     5581 acgccttatc cagcctacaa aaactcataa attcaaagtg ttgcaggaaa aggtaggcct
     5641 gataagcgta gcgcatcagg caatctctgg tttgttttca gatgaaaacg ccgaagtgaa
     5701 aattctccgg cgttttggcc gtgaattact gctgcggaat tgccggtaca gccggaacgt
     5761 taagagctgg catcgcaaac ataccaacga aatcttccag tggcattttc tgcccgttta
     5821 acgttatctg accgttagta tattgcaggc tggtggtgat ggtattgtcc tgcaaggtgg
     5881 tcagacggaa catctgcccc attgctgatg caccttcaac ttgctgtttc gccagttttt
     5941 tcgcttgatc ttcctgataa ccttccagct tcgctacctg agtcataaac tcagttgcca
     6001 tatccaccgg aatggtcagt ttcgcatcca gagatttaac cgaacgatct acttcctgcg
     6061 ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt caggaacagc gacagattca
     6121 gcgcactttc accctgactg tttttccagc ttagcggcgc gatagtaatc accggatcgc
     6181 ctttcaacat cagcggcagg gcgctaaaga aggcttccgt cactttctcc tgataaagtt
     6241 cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg agtttgcgcg ttatattgct
     6301 ggctaaactg atgccaggct tcaccatcaa tctggccgac ttttaaagtc agcttgccgc
     6361 tgcccagatc ctgattctgt accttcaggc tgtttagcga gtaatccagt tggctattga
     6421 tcgttttacc gtcattgacc agatccgatt taccgctgat ctccatgcct tccagcagtg
     6481 ccagttcttt gccttccact gaaatggtca ttttttccag tgacagtttt tggtttccta
     6541 cgcgctcacc aaaacttgcc agcgtgctgg aaccgtcggt tttcagatta ttaaaggtca
     6601 actgcacttt ctggttgtat tcgttaactg cgtctatccg accactttgc gcctccccgg
     6661 aaagggagat ggctttgccg tctctgtcag catttaactg gaactcgccg ccgctaaagg
     6721 cgactttttc atccttttgc tcgtaattca gtggcttgag cgaaatatcg gaactggaat
     6781 caccgctgta accaatgcgc gagttaatct caaaaggcgt ttcacctttt gccatatcaa
     6841 acagtggttt gcttacttcg ttattaacca gcgtggtttg aattgatgcc atcgacggga
     6901 tcaggttcag ttttttaagc tgggcaagcg ggaagggacc atgatcaacc gattcgttga
     6961 agatgacgct ctgaccgctt ttaatccacg gattttcttt cccggcaatg ggtttcacca
     7021 acagttgcaa ctggctgctg aatacgccgc gatgatagtt ttgataactc acttccaggt
     7081 tggattcagg agctgtcagt ttgagttgcg cgttcgcctg cgcgaccatg tcttcgagat
     7141 gggtttcaat cttcttgcct gtataccatg cgccgcctgt ccagactacg cctagcgcaa
     7201 caatgacgcc taccgctacc agcgatttat tcataatgat tatccataaa atgaaatcag
     7261 gcggactggc cgcctgaagg tgttataagc ctttaataag cttagcaaga gatgttaatt
     7321 ttttcagtaa gctcttacag cttgttgtaa acacgcgcta aacggccgtg gcctttgaca
     7381 gtcaccggtg attcgttggc ggcaataaac gctgattcac ccggtttaag ctgtaactgc
     7441 tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga acaaaatggc ggcactctgc
     7501 tggctaatgg tggtttcttt atcactaagg tcatgcagcg agaaggcaaa atcatccact
     7561 ggaatcggga agtccagttc tgcaccttgt ttcaccggct gggtcaacaa ctggttagcc
     7621 ggtttggctt cgaatttcac attggcaacc agttccggaa tatcaatgta cttaggcgtc
     7681 agacccgcac gcagcacgtt atcggagttc gccatcactt ccagcgccac gccttgcagg
     7741 taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc caggattcaa tttcaccaca
     7801 ttcagtaaca gcggggagaa caggccactg tcttccgggt aaaattcaga aattaaacga
     7861 atcgtttgcc acggttcacc ctgctgactg tcgagcgccg attttaaaat cgccagcgcg
     7921 cgggattttt cttcaccctg catattcaac aggctggcga acagttcgct taaacgttcg
     7981 gcattaggct gttgtaaaaa gtgagcaatt gccggatgtg cacctgcgac tggctggagt
     8041 agggagacaa tctcggaaaa ctcacgaaac gcgttcatcg caaggaaagg cgtcagcgca
     8101 aaaaccagct ccggcttgtg gttaggatct ttatagttac gctcggcggc atccatcggg
     8161 atacctgcgg cattttcttt ggcaaaaccg atttcagaat tgcgtttgtt tggatgaacc
     8221 tgaatggaga gcggctgtgc tgcgcataat actttgaaca ggaaaggcag ttcgccaaag
     8281 cgtttggcaa cggcctctcc gagcagagtc gatttatcac tctcaatcac atcacgcagt
     8341 gaaacgatat ctccggcggc attctgcact cgtgaactgc ttttcggatg tgcgcccatc
     8401 cacagctcgg ccatcggctg gctggacgga ttttccatac cataaagttc agtcaacgcc
     8461 gttttgctgc cccaggcata gttttgcact gagttaatga gtttttgcat gatcaatccc
     8521 tgttttaatg tggaaattaa tcccactatt aaagcaagaa tcctacggga agtaacctgg
     8581 agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa agtgtgtagg atattgttac
     8641 tcgcttttaa cagggcaacg gaacacccgc ccagagcata accaaaccag gcagtaagtg
     8701 agagaacaat gtcaaacaaa ccctttcatt atcaggctcc ttttccactc aaaaaagatg
     8761 atactgagta ttacctgcta accagcgaac acgttagcgt atctgaattt gaagggcagg
     8821 agattttgaa agtcgcaccc gaagcgttaa ctctgttggc gcgtcaggcg tttcatgatg
     8881 cgtcatttat gctgcgtccg gctcaccaac aacaggtggc cgacattctg cgtgacccgg
     8941 aagccagcga aaatgataaa tatgtggcgc tgcaattcct gcgcaactcc gacatcgcgg
     9001 cgaaaggtgt tctgccaacc tgtcaggata ccggcactgc gattattgtt ggtaaaaaag
     9061 ggcagcgtgt atggaccggt ggctgtgatg aagcggcgct ggcgcgcggt gtctataaca
     9121 cttatatcga agataatttg cgctactcgc aaaacgcgcc gctggatatg tataaagagg
     9181 tgaataccgg caccaatctg ccagcgcaga tcgatcttta tgccgttgat ggcgacgagt
     9241 acaaattcct ctgtatcgcc aaaggtggtg gttcggcaaa caagacgtat ctctatcagg
     9301 aaaccaaagc gttactaacg ccggggaaac tgaaaaatta cctggttgag aagatgcgca
     9361 cgctgggtac ggcggcctgt cctccgtatc atattgcgtt cgtaattggt ggaacttctg
     9421 cagaaactaa ccttaaaaca gtgaaactgg cttccgctaa atactatgac gaactgccaa
     9481 cggaagggaa tgagcacggt caggcattcc gcgatgtgga actggaaaaa gaattgctga
     9541 tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg taaatacttc gctcacgaca
     9601 tacgcgtgat tcgcctgcca cgtcacggcg catcctgtcc ggttggtatg ggtgtttcct
     9661 gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca ggggatctgg atcgaaaaac
     9721 tggaacataa tccaggtaaa tatatccctg aagagctgcg taaagcggga gaaggcgaag
     9781 cggtgcgcgt tgaccttaac cgtccgatga aagagatcct cgcacagttg tcgcagtatc
     9841 ccgtttctac acgcttatcg cttaacggca cgattatcgt cggtcgtgat attgctcacg
     9901 ccaaactgaa agagcggatg gataacggtg aagggctgcc gcagtacatt aaagatcatc
     9961 cgatttacta cgcgggtccg gccaaaacgc cggaaggtta tgcctccggt tctcttggcc
    10021 caacgaccgc cggacggatg gattcttatg tcgatcaact gcaagcgcag ggcggaagta
    10081 tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac ggatgcctgt aaaaaacacg
    10141 gcggcttcta ccttggcagt atcggtggtc cggccgctgt attggcgcag ggaagtatta
    10201 agagcctgga atgtgttgaa tatccggaac tgggaatgga agccatctgg aaaattgaag
    10261 tggaagattt cccggcgttt atccttgtgg atgataaagg aaatgacttc ttccagcaga
    10321 tacaactcac acagtgcact cgctgtgtga aataaacaga gccgccctt
//
LOCUS       DAQSWQ010000140        10342 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.142, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000140 DAQSWQ010000000
VERSION     DAQSWQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.142"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(43..1047)
                     /gene="cra"
                     /locus_tag="R8G18_003737"
     CDS             complement(43..1047)
                     /gene="cra"
                     /locus_tag="R8G18_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HEE9797334.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1129..1215)
                     /locus_tag="R8G18_003738"
     CDS             complement(1129..1215)
                     /locus_tag="R8G18_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HEE9797335.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1227..1718)
                     /gene="ilvN"
                     /locus_tag="R8G18_003739"
     CDS             complement(1227..1718)
                     /gene="ilvN"
                     /locus_tag="R8G18_003739"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEE9797336.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1721..3445)
                     /gene="ilvI"
                     /locus_tag="R8G18_003740"
     CDS             complement(1721..3445)
                     /gene="ilvI"
                     /locus_tag="R8G18_003740"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HEE9797337.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3564..3608
                     /locus_tag="R8G18_003741"
     CDS             3564..3608
                     /locus_tag="R8G18_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797338.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3763..4707)
                     /gene="leuO"
                     /locus_tag="R8G18_003742"
     CDS             complement(3763..4707)
                     /gene="leuO"
                     /locus_tag="R8G18_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HEE9797339.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5367..5453
                     /gene="leuL"
                     /locus_tag="R8G18_003743"
     CDS             5367..5453
                     /gene="leuL"
                     /locus_tag="R8G18_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HEE9797340.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5546..7117
                     /gene="leuA"
                     /locus_tag="R8G18_003744"
     CDS             5546..7117
                     /gene="leuA"
                     /locus_tag="R8G18_003744"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEE9797341.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7117..8208
                     /gene="leuB"
                     /locus_tag="R8G18_003745"
     CDS             7117..8208
                     /gene="leuB"
                     /locus_tag="R8G18_003745"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEE9797342.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8211..9611
                     /gene="leuC"
                     /locus_tag="R8G18_003746"
     CDS             8211..9611
                     /gene="leuC"
                     /locus_tag="R8G18_003746"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEE9797343.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9622..10227
                     /gene="leuD"
                     /locus_tag="R8G18_003747"
     CDS             9622..10227
                     /gene="leuD"
                     /locus_tag="R8G18_003747"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEE9797344.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2709 a         2719 c         2526 g         2388 t
ORIGIN      
        1 cgccagaggg aaattcacct ggcgcgtatt tttgttcgcg gcttagctac ggctgagcac
       61 accgcggcga tagagattac gtttaatgcg cgttaaacca ggttttggct tacgcggttc
      121 gtccaggctt gccaggacaa tctccagcac acgctctgcg acatcgcggt gacgttgagc
      181 cactgccagc accgggcact gtaagaagtc gagcagttcg ttatcgccaa aggtggcaat
      241 tgccaggtca gaaggcagtt taccatcgcg acgtagcgtg acatccatca ccccttgcaa
      301 caacgcaaac gacgttgtga acagcgcctg cggcatcgga tgcgtttcca gccatttttc
      361 gaataactgg gcagccacct cccgctcata gctgttggca tacaggaaat gcacttcgcg
      421 cggatcatct ttccaggcag tacggaaacc ttgttcacgc aggaagctga cagaaagctc
      481 cggcagcgcg ccaagataaa gcaccgtctc ggcgggaaac ttacgtaact cttccgccag
      541 catttcggca tcatcctgat cggcaccaac cacgctggtg aagtgttcac gatcgagggc
      601 gcggtccagc gcgacaatcg ggaacgggtc gttagcccag cgttgataaa aaggatgctc
      661 aggaggcaac gacgtcgaaa caataatggc atcgacctga cgctgtaaaa ggtgctcaat
      721 acagcgcatt tcgttgtctg gctgatcttc tgagcaggca attagcagtt gataaccccg
      781 ttgccgcgcc tggcgttcaa gatagttagc gatgcgggta tagctggtgt tctccagatc
      841 ggggatcaca agaccaatag aacgtgtgcg tccagcacga agcccagctg ccacggcgtt
      901 cgggtggtaa ttgtgctcac gcaccacagc catgactttt tcaacggttt tgtcgctcac
      961 acggtattgc ttcgctttgc cgttaataac atagcttgca gtggtccgcg acactcccgc
     1021 cagccgagcg atttcatcca gtttcacaat tgccccttgc gtaaaatgta aaaaccatag
     1081 ccattgtact ggtatgggtt aaattcatta acatctaagc gcataaaatt aactgcggca
     1141 actgctttta ttccaggttc ccactgattt cgcaaaaaaa gcccaacgtg acatgttggg
     1201 ctgcaaattg cgcattcaga tcattctcaa cgcattattt tatcgccgcg cgaaagtccg
     1261 accacaccag aacgagcaac ttccacaatt ttcgccacct cgcgaatcga tgctaaaaat
     1321 gcatcaagct tatcgctggt gcctgctaat tgaacggtat aaagcgaggg tgtgacatcg
     1381 ataatttgcc cacggaatat ttccgtatta cgtttcactt cgtcacgccc gtaaccgctg
     1441 gcctgaattt tcaccagcat gatttcccgc tcaacatgcg cgccctgccc caactcactc
     1501 acgcgcaaga catcgaccag cttgtgtaat tgcttttcga tctgctcaag tactttttca
     1561 tcgcccacgg tctggatggt catacgcgat aatgtcggat catcggttga cgcaacggtc
     1621 aggctttcaa tgttgtagcc acgctgggaa aaaaggccaa tcacgcggga taacgcgcct
     1681 gattcatttt cgagtaagac tgataatatc cggcgcataa tcaggttctc tccgttttgc
     1741 ttaaccacat ttcatccatt ccgcccccgc gaatctgcat cgggtagacg tgctcgctgc
     1801 catcgacggt aacatcaaca aacaccaggc gattattgtg cacctgttcc agcgcctcgc
     1861 taagtttgct ttccagctca tgcggatgag aaatctggat cccgacgtga ccataggctt
     1921 ccgccagacg gacgaaatcg ggtagcgatt gcatataaga ttgtgaatga cggccggaat
     1981 agatcatgtc ctgccactgc ttcaccatcc ccagatagcg gttattgaga ttcaccacca
     2041 gtacgggcaa ctcgtattgc aacgcggtag acagttcctg gatgttcatc tgaatactgc
     2101 cgtcgccagt gacgcaaacc acggtttctt ctggcaacgc cattttgacg cccagtgccg
     2161 caggtaaacc aaaacccatc gtgccgaggc caccggaatt gatccagcga cgcggtttgt
     2221 cgaatggata ataaagtgca gcaaacatct ggtgctgccc gacatcggac gtcacgtaag
     2281 cgtctccctt cgtcaaccgc caaagagtct cgatcaccgc ctgcggttta atcttttcac
     2341 tgtgagtgtc atatttcagg cactgacgag cgcgccactg ttcaatttgc tgccaccagt
     2401 cgcggatctc atccagtggt tgatgggcgg attcttgcga caagagttca agcatttgtt
     2461 cgaggacctg gcgagcatcc cccacaatcg ggatatccgc agtcacggtt ttagaaatgg
     2521 aagtaggatc aatatcgata tgcagaacag tggcatttgg gcagtacttt gccagattgt
     2581 tcgtcgttcg gtcatcaaat cgtaccccga cggcgaaaat cacatccgcg ttatgcatcg
     2641 tcatattggc ttcgtaggta ccgtgcattc ccagcatgcc cagtgcctga cgatgcgttg
     2701 ccggaaacgc tcccagcccc atcaatgagg aaacaacggg cagattcaac gcctccaccg
     2761 tttctttcaa ctgctgatgg cagcccgccg tgattgcccc accgcctacg tagacaaccg
     2821 gtttttttgc cgctaccagc gtttgcagag cgcgcttaat ttgcccttta tgtccggtag
     2881 tagtgggatt gtaagaacgc atactgaccg actccggcca gacatagggt aatttgttcg
     2941 ccggattaag aatatctttc ggtaaatcaa cgactactgg tccaggacga ccacttgccg
     3001 ccagccagaa agcctttttc agcacctgcg gaatgtcttc cgtttgctta accagaaaac
     3061 tgtgtttaac cactggtcgc gaaatcccca ccatgtcgca ctcctgaaag gcatcgtaac
     3121 ctatcaacga ggtcgctacc tgcccggaaa ggacaactaa tggaatggaa tccatataag
     3181 cggtggcgat gccagtaatc gcattggtcg cccctggacc cgacgttacc agcacgacgc
     3241 cgacttcccc ggtcgcgcgc gccagaccat cggccatatg cactgccgcc tgctcatgac
     3301 gaactaaaac atgatcgata ccacccacgg tatgcaatgc atcgtaaata tcaaggactg
     3361 cgcctccggg ataaccgaat acttgtttaa cgccctgatc gataagcgat cggacgacca
     3421 tctcggctcc agacaacatc tccatggcct gcctcactgt ttgacggaaa aaatgtgtaa
     3481 aacacattaa ccgtccgatc ttgcttaagc aattggcagc cagagacata aacgcagggt
     3541 aataagaata aacaggagga aaggtgaaaa gaaacgataa aatcctccat tacgaaggat
     3601 taaattgata ataaatcggc tgaatcccac aacttacagc aatttctcta tttttttaat
     3661 taatcctaaa atagagagcc cacacattca gaataaaatt ctacattcaa taaaaaatac
     3721 agaataaacc agacattcat gtctgaccta ttctgcattc agttagcgtt tgcaaattga
     3781 gactaattgc tcttccatcc actgatggcc tttatcgcgc ccggcagctt catgccagga
     3841 gagataacag gttctgctat tttgttttaa cggcagcggt aatacctgta attctaagga
     3901 ttcagcgaac tcttcagcca gccaacgcgg cgcaatagcg accaaatgcg tttgcgacac
     3961 cacgctaagt acgctcatca ttgccatgcc ctgatacgcg atactggctt gcttatctac
     4021 cgtgtcatac caaggttgac taaatgacgc gaaacgatcg agcgaaaccg ccgcatgttg
     4081 ttcgttataa acatcatgtt tcagtaacgg gcccttaatt gttggatgat ttttgctggc
     4141 taccagcacc atttcatctt taaataatgg cacgctggta aattcaggac gatggaagtc
     4201 ttcataacta atcacaaact ccgtttcctg ataacgcagc tgatgttcag tgttctgatt
     4261 taatgaagac ttgaacataa catgtatatt tggcgcaatc tgctcaatgt gattataaat
     4321 ctgcgaggtc agaatgctgt ctaacgggct gcaaacacaa agatgaaata cacgttcact
     4381 gctcgcgggt tcaaaacctg aaccaggcaa ttcattttgt actagttgca atgcctgacg
     4441 aactgagcca aaaagttgaa atgcgcgagc agtcggttga ataccacggc cataacgaac
     4501 aaaaagctcg tcattaaaca tcaccttcag gcgtgcaaca gcgttactga ccgcaggttg
     4561 cgacataccc agaacatgag cggcacgggt aatgttttgc tcctgcatca cggcatcgaa
     4621 aacggttaat aagttgagat cgaccatgcg tagctgtggc ttgcttaact ccgccgtctc
     4681 tgaatgatct gtttgtacct ctggcatact taactccact gtcacactta actccctttc
     4741 ccttattgga atgcagagaa ataatcctga agaatatgat ttatcatgca tataaaataa
     4801 gaaaaagcaa aatgagtaaa attcggaaaa cataaagata cgggttctga taaaaacaga
     4861 atccattgcc gtggaatgag tcatttacca cagcataaga atccataatg tttttgttgc
     4921 gaaaacaatc taatcataac taccgcgaat actcaatcat ctacaaaatg gattaaatgt
     4981 gcttatttaa taattaattt atatatttaa tgcattaatt cttaacatta attgatcaat
     5041 aatattcacc aaatcaatat caaaaaaaat cgcaaaacat ataattcaat acaaatcatc
     5101 aggataggtt ttgcaacgcg tgcattttgt cccctttttc ctcgttgatt agatgcaaaa
     5161 atttatgctg aaatatgtca accgatgaaa agcgtcggta gttaagcaga aattaatatc
     5221 gcttacttta accaccgcag cacaattagc taattttacg gatgcagaac tcacgctggc
     5281 gggacgtttt tattgcgtca gggttgacat ccgtttttgt atccagtaac tctaaaagca
     5341 tatcgcattc atctggagct gatttaatga ctcacatcgt tcgctttatc ggtctactac
     5401 tactaaacgc atcttctttg cgcggtagac gagtgagcgg catccagcat taagccagca
     5461 cgcagtcaaa caaaaaaccc gcgccattgc gcgggttttt ttatgcccga agcgaggcgc
     5521 tctaaaagag acaaggacct aaaccatgag ccagcaagtc attattttcg ataccacatt
     5581 gcgcgacggt gaacaggcgt tacaggcaag cttgagtgtg aaagaaaaac tgcaaattgc
     5641 gctggccctt gagcgtatgg gtgttgacgt gatggaagtc ggtttccccg tctcttcgcc
     5701 gggcgatttt gaatcggtgc aaaccatcgc ccgccaggtt aaaaacagcc gcgtatgtgc
     5761 gttagctcgc tgcgtggaaa aagatatcga cgtggcggcc gaatccctga aagtcgccga
     5821 agccttccgt attcatacct ttattgctac ctccccaatg cacatcgcca ccaagctgcg
     5881 cagcaccctg gacgaggtga tcgaacgcgc tatctatatg gtgaaacgcg cccgtaatta
     5941 caccgatgat gttgaatttt cttgcgaaga tgccgggcgt acacccattg ccgatctggc
     6001 gcgagtggtc gaagcggcga ttaatgccgg tgccaccacc atcaacattc cggacaccgt
     6061 gggctacacc atgccgcttg agttcgccgg aatcatcagc ggcctgtatg aacgcgtacc
     6121 taacatcgac aaagccatta tttccgtaca tacccacgac gatttaggcc tggcggtcgg
     6181 caactcactg gcggcggtac atgccggtgc acgccaggtg gaaggtgcaa tgaacgggat
     6241 cggcgagcgt gccggtaact gttcgctgga agaagtcatc atggcgatta aagttcgtaa
     6301 ggatattctc aacgtccaca ccgccattaa tcaccaggag atatggcgca ccagccagtt
     6361 agttagccag atttgtaata tgccgatccc ggcaaacaaa gccattgttg gcagcagcgc
     6421 attcgctcac tcctccggta tccaccagga tggcgtgctg aaaaaccgcg aaaactacga
     6481 aatcatgaca ccagaatcta ttggtctgaa ccaaatccag ctgaatctga cctctcgttc
     6541 ggggcgtgcg gcggtgaaac atcgcatgga tgagatgggg tataaagaaa gtgaatataa
     6601 tttagacaat ttgtacgatg ctttcctgaa gctggcggac aaaaaaggtc aggtgtttga
     6661 ttacgatctg gaggcgctgg ccttcatcgg taagcagcaa gaagagccgg agcatttccg
     6721 tctggattac ttcagcgtgc agtctggctc taacgatatc gccaccgccg ccgtcaaact
     6781 ggcctgtggc gaagaagtca aagcagaagc cgccaacggt aacggtccgg tcgatgccgt
     6841 ctatcaggca attaaccgca tcactgacta taacgtcgaa ctggtgaaat acagcctgac
     6901 cgccaaaggt cacggtaaag atgcgctggg tcaggtggat attgtcgcca actacaacgg
     6961 tcgccgcttc cacggcgtcg gcctggccac cgatattgtc gagtcctccg ccaaagccat
     7021 ggtgcacgta ctgaacaata tctggcgtgc cgcagaagtc gaaaaagagt tgcaacgcaa
     7081 agctcaacac aacgaaaaca acaaggaaac cgtgtgatgt cgaagaatta ccatattgcc
     7141 gtattgccgg gggacggtat tggtccggaa gtgatgaccc aggcgctgaa agtgctgaat
     7201 gccgtgcgca accgctttgc gatgcgcatc accaccagcc attacgatgt aggcggcgca
     7261 gccattgata accacgggca accactgccg cctgcgacgg ttgaaggttg tgagcaagcc
     7321 gatgccgtgc tgtttggctc ggtaggcggc ccgaagtggg aacatttacc accagaccag
     7381 caaccagaac gcggcgcgct gctgcctctg cgtaagcgct tcaaattatt cagcaacctg
     7441 cgcccggcaa aactgtatca ggggctggaa gcattctgtc cgctgcgtgc agacattgcc
     7501 gctaacggct tcgacatcct gtgcgtgcgc gaactgaccg gcggcatcta tttcggtcag
     7561 ccaaaaggcc gcgaaggtag cggacaatat gaaaaagcgt ttgataccga ggtgtatcac
     7621 cgttttgaga tcgagcgtat tgcccgcatc gcgtttgaat ctgctcgtaa acgtcgccac
     7681 aaagtcacct caatcgacaa agctaacgtg ctgcaatcct ctattttatg gcgtgaaatc
     7741 gtcaacgaga tcgccacgga atacccggat gtcgaactgg cgcatatgta catcgacaac
     7801 gccaccatgc agctgattaa agatccatca cagtttgacg ttctgctgtg ctccaacctg
     7861 tttggcgaca ttctgtctga cgagtgcgca atgatcactg gctcgatggg gatgttgcct
     7921 tccgccagcc tgaacgagca aggttttggt ctgtatgaac cggcaggcgg ctcagcacca
     7981 gatatcgcag gcaaaaacat cgccaacccg attgcgcaaa ttctgtcgct ggcactgctg
     8041 ctgcgctaca gcctggatgc cgatgatgcg gcttgcgcca ttgaacgcgc cattaaccgc
     8101 gcattagaag aaggcattcg caccggggat ttagcccgtg gcgctgccgc cgttagtacc
     8161 gatgaaatgg gcgatatcat tgcccgctat gtggcagaag gggtgtaatc atggctaaaa
     8221 cgttatacga aaaattattc gacgctcacg tagtgtacga agccgaaaac gaaaccccgc
     8281 tgttatatat cgaccgccac ctggtgcatg aagtgacctc accgcaggcg ttcgatggtc
     8341 tgcgcgccca cggtcgcccg gtacgtcagc cgggcaaaac cttcgccact atggatcaca
     8401 acgtctctac ccagacgaaa gacattaatg cctgtggtga aatggcgcgc atccagatgc
     8461 aggagctgat taaaaactgc aaagaatttg gcgtcgaact gtatgacctg aatcacccgt
     8521 atcaggggat cgtccacgta atggggccgg aacagggcgt caccttgccg gggatgacca
     8581 ttgtctgcgg cgactcgcat accgccaccc acggcgcgtt tggcgcactg gcctttggta
     8641 tcggcacttc cgaagttgaa cacgtactgg caacgcaaac cctgaaacag ggccgcgcaa
     8701 aaaccatgaa aattgaagtc cagggcaaag ccgcgccggg cattaccgca aaagatatcg
     8761 tgctggcaat tatcggtaaa accggtagcg caggcggcac cgggcatgtg gtggagtttt
     8821 gcggcgaagc aatccgtgat ttaagcatgg aaggtcgtat gaccctgtgc aatatggcaa
     8881 tcgaaatggg cgcaaaagcc ggtctggttg caccggacga aaccaccttt aactatgtca
     8941 aaggccgttt gcatgcgccg aaaggcaaag atttcgacga cgccgttgcc tactggaaaa
     9001 ccctgcaaac cgacgaaggc gcaactttcg ataccgttgt cactctgcaa gcagaagaaa
     9061 tttcaccgca ggtcacctgg ggcaccaatc caggccaggt gatttccgtg aacgacaata
     9121 ttcccgatcc ggcttcgttt gccgatccgg ttaaacgcgc gtcggcagaa aaagcgctgg
     9181 cctatatggg gctgaaaccg ggcattccgc tgaccgaagt ggctatcgac aaagtgttta
     9241 tcggttcctg taccaactcg cgcattgaag atttacgcgc ggcagcggag atcgccaaag
     9301 ggcgaaaagt cgcgccaggc gtgcaggcac tggtggttcc cggctctggc ccggtaaaag
     9361 cccaggcgga agcggaaggt ctggataaaa tctttattga agccggtttt gaatggcgct
     9421 tgcctggctg ctcaatgtgt ctggcgatga acaacgaccg tctgaatccg ggcgaacgtt
     9481 gtgcatccac cagcaaccgt aactttgaag gccgccaggg gcgcggcggg cgcacgcatc
     9541 tggtcagccc ggcaatggct gccgctgctg ctgtgaccgg acatttcgcc gacattcgca
     9601 acattaaata aggagcacac catggcagag aaatttatca aacacacagg cctggtggtt
     9661 ccgctggatg ccgccaatgt cgataccgat gcaatcatcc cgaaacagtt tttgcagaaa
     9721 gtgacccgta cgggttttgg cgcgcatctg tttaacgact ggcgttttct cgatgaaaaa
     9781 ggccaacagc caaacccgga cttcgtgctg aacttcccgc agtatcaggg cgcttccatt
     9841 ttgctggcac gagaaaactt cggctgtggc tcctcgcgtg agcacgcgcc ctgggcattg
     9901 accgactacg gctttaaagt ggtgattgcg ccgagttttg ctgacatctt ctacggcaat
     9961 agctttaaca accagctgct gccggtgaaa ttaagcgatg cagaagtgga cgaactgttt
    10021 gcgctggtga aagccaatcc ggggatccat ttcgacgtgg atctggaagc gcaagaggtg
    10081 aaagcgggag agaaaaccta tcgctttacc atcgatgcct tccgccgcca ctgcatgatg
    10141 aacggtctgg acagtattgg gcttaccttg cagcacgacg acgccattgc cgcttatgaa
    10201 gcaaaacaac ctgcgtttat gaattaatcc ccttgcccgg tcaaatgacc gggctttccg
    10261 ctatcgtcca cgtcatcaaa cgtctttaac ctttgcggtt aaaaataatg cgacaacaga
    10321 aataaccgca attacccagt ag
//
LOCUS       DAQSWQ010000141        10068 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.143, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000141 DAQSWQ010000000
VERSION     DAQSWQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.143"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(387..701)
                     /locus_tag="R8G18_003748"
     CDS             complement(387..701)
                     /locus_tag="R8G18_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HEE9797345.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(706..921)
                     /locus_tag="R8G18_003749"
     CDS             complement(706..921)
                     /locus_tag="R8G18_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HEE9797346.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(1072..2925)
                     /locus_tag="R8G18_003750"
     CDS             complement(1072..2925)
                     /locus_tag="R8G18_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HEE9797347.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            complement(3901..3977)
                     /locus_tag="R8G18_003751"
     tRNA            complement(3901..3977)
                     /locus_tag="R8G18_003751"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3941..3943),aa:Arg,seq:tct)
     gene            complement(3991..4067)
                     /locus_tag="R8G18_003752"
     tRNA            complement(3991..4067)
                     /locus_tag="R8G18_003752"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4031..4033),aa:Arg,seq:tcg)
     gene            complement(4075..4150)
                     /locus_tag="R8G18_003753"
     tRNA            complement(4075..4150)
                     /locus_tag="R8G18_003753"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4115..4117),aa:Ile,seq:cat)
     gene            complement(4191..5249)
                     /locus_tag="R8G18_003754"
     CDS             complement(4191..5249)
                     /locus_tag="R8G18_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEE9797348.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5357..5597)
                     /locus_tag="R8G18_003755"
                     /pseudo
     CDS             complement(5357..5597)
                     /locus_tag="R8G18_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5822..6643)
                     /locus_tag="R8G18_003756"
     CDS             complement(5822..6643)
                     /locus_tag="R8G18_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HEE9797349.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(6640..7020)
                     /locus_tag="R8G18_003757"
     CDS             complement(6640..7020)
                     /locus_tag="R8G18_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEE9797350.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(7021..8007)
                     /locus_tag="R8G18_003758"
     CDS             complement(7021..8007)
                     /locus_tag="R8G18_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HEE9797351.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(8081..8359)
                     /locus_tag="R8G18_003759"
     CDS             complement(8081..8359)
                     /locus_tag="R8G18_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797352.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            complement(8426..8686)
                     /locus_tag="R8G18_003760"
     CDS             complement(8426..8686)
                     /locus_tag="R8G18_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797353.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFISRANEFVTSLPSLKEAGIKAGFSAFEDE"
     gene            complement(8907..9062)
                     /gene="hokD"
                     /locus_tag="R8G18_003761"
     CDS             complement(8907..9062)
                     /gene="hokD"
                     /locus_tag="R8G18_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HEE9797354.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(9354..9587)
                     /locus_tag="R8G18_003762"
     CDS             complement(9354..9587)
                     /locus_tag="R8G18_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797355.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            complement(9695..9862)
                     /locus_tag="R8G18_003763"
     CDS             complement(9695..9862)
                     /locus_tag="R8G18_003763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797356.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            complement(9873..>10068)
                     /locus_tag="R8G18_003764"
     CDS             complement(9873..>10068)
                     /locus_tag="R8G18_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797357.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
BASE COUNT         2391 a         2795 c         2301 g         2581 t
ORIGIN      
        1 gtcagcttct tatcgaactt gagctgaagg atcttaaaac aagtttccat agtgctcctg
       61 gattttctct ttttggacat tgacgtctcc actggtttaa acacggcaat ggagactgtg
      121 gtgaaaagag ttaattcccg gagtgactgg cgaattccaa tcaatgatcg gaagtatgcc
      181 aaatcgtgat cgattgtgat gccaaactgt gatcggagat ggatgccaaa tcatgatcga
      241 ttcgaatgcc aaactgcgat cgattcagat gccattttgc aatcagcgcc agaacagcag
      301 ctgacaggcc gtatttgatt tttgcgctca tggatattta tcaggatgct accaatgaaa
      361 gatactggaa agccaactgc aaaaagctaa caacccgtaa tcgagttatc agaactgtta
      421 atttttatgg tataccgcgc ctctgaacag gggcgcgttt ctggcaacag ctcgtcccct
      481 tcacataacc cggcagcaac atccaagaag acctgtctga tgctccttct ggctgctgcc
      541 tcataaaact ccagcgcggc accttcaaca cggtccagcg agatgtccag gtcaaaaatt
      601 tcaccgtcaa agcgtttttt gtcccgtaac gctaaagtta ccgtaacttt attctcaaaa
      661 ttgcggatcc ctttcacaat cagttcatag ttttgagtca ttgaattact ctccccgtgc
      721 agccttacga cggtcctctc tgattttgaa atacaggtta gtcagatatg tcagcagccc
      781 aaacagcaga ctccccagca cgcctattgc cgcccactga gacggggaaa ccctgtccag
      841 caactgcagg aaccagtagc ccgttcccac cgctgacgtg gtgtatgaca cacctgttgt
      901 gattttttcc atctggtaca taccccgtct cccgttatcc ggaagctgac aacaataaaa
      961 aaagccacca gttaagtact gatggctctg ataactcatg caggcatctc agacgaccca
     1021 ctgacactac cggtgagttt aacgatacct tccatttggc tggctcactt tttatgatga
     1081 tgccggtgca tttatctcca gcaccagact ttctatctca acgccatacg ctgcattttt
     1141 ggtaatatcc gtcagcgtca gcgcattcag ccccagtgtc agactgtctt ttatgacctg
     1201 gaatgccggg ccagccactc cattcagttt cggagtaacc gtggcactgc cggcggtgaa
     1261 caccagctcc agcgtcagcc agtcgttact gtaattcccg aactcgccca actttgtgtt
     1321 tcctgctttc ttgtgatgca tcagattcag tttgccgtct gtggtctggg tgaagaacga
     1381 catcaggaac gggttaccag tcccggtcat cgccacgacg tcaggtaacg ctacatcggt
     1441 atacagataa attcccagac cgaactggtt gttggtcagt gcgcctgaca gtcgaaactt
     1501 acagctcagt ctgccacccc gtgtcagcag ggagactgcg tcatccaccg ggcgcgtcag
     1561 ggaccaggct ttattgctct gcttggtgat cttaaataca ccatctgaca actgaactcc
     1621 gccattctta atgctccagc cctgcgcagc agcgtctccg gctgtcggca acagggagat
     1681 tgtgcgtatg gatgcatctt cagacggccc cgatggcgtg tcgccgccgg gcgagggttt
     1741 gatttccggt gccttaccac taatgaaggc taaggtgcga ccggctacgt tcagaatagc
     1801 agttgccata cgatcgggaa taatgccacg acgcgcccat gagctgaaat gcgtcgggcg
     1861 atttgatgat acccagtttt tgttcgttcg ggatgccgaa ccgtaataac cagacccggc
     1921 aatatcagga tcttctgacg ggttgtttgt cggtgtatta actccgctac catcggtcat
     1981 aaagggaaca aaataaatct gctgggattc tttaccttta tatgcaccat ataccacttc
     2041 atattgcgta ccgtgttctt gtttccacgc gtatgtcgtg tcgccacaaa tccaggggac
     2101 tgatgccgga cttccaccgt gacactgcgc cgccagcccg gcaaggtcag cacggaactg
     2161 ctgtaccatt gcaagaaatg ctgctggctg ctgggcgtaa ctggcattcg tcatatcgaa
     2221 ttccccctgc atccagcata tcgccagcaa aacgtttttc gggtttttct gcaatgctgc
     2281 cttcgtgcgg aaaagcagat cctgatataa cggcttaccc actccccagc gagccgaatc
     2341 ctgactggcc cccgtggact cgctgaatgt cccctccgtg ccctgggtga atgccgaacc
     2401 accacaacag catggtacca gcaggatccc cgcattatta gggatatacg gaagcagttt
     2461 tttggcaata tgtaagccct gtccgacaca gccgtactgc cctttgctca ggtcagcccg
     2521 gggatggtta atcgtactca tatcctgaac atcatgcaga caatggtcag caggaatgat
     2581 gtcgttaaat acgcatactt caccaccggg agtcactgtg ttacgacggg ccagttgctt
     2641 aatgcgcgga tggggcgcat cgtatgaatc cggaagcgga agaccttcac cgtaagccat
     2701 ggcattggat tgcccggcca gtacgatgac gtagtaccac tccggctcag ttgcaccact
     2761 gacgaccaca tcaccttctg ctgcaatcgc ctgcatcagg gtataagggg ttatggccac
     2821 cggactacca aacggctgcc agccctcctt cagtttttgt gtcagtcgtt tcgcaaggtc
     2881 tgacggcgat gccgccctga ccacgtcata gtgtttaaat gccatgaatc ctcccggccg
     2941 ggataatatt gtgagtaaaa taaggagcgg gctgaagtcc ggaagttaca ggacaatggc
     3001 agaagagaga cgacagcccg caattcgaaa aagaccgcgc agttgcgcag agtgattact
     3061 atggggtatt attcgccagc tgaaatatta cttcacgttt tattgtttat tccttgccgc
     3121 ccgcgtctcc cagcgcgggc tttttttgcc cacaagaaaa cccctccgga gaggggctaa
     3181 agccgcgtat ctgtatcatc atgcacatgg tgccgggtgc ctcccggtga gttcagcccg
     3241 gtgccactaa acccgcgtca ttctcgtttt gataatcaga gattataccg tcaccagtcg
     3301 cccctccgct cagggggatt caccatgcga aattttttta acaaatgccc agtctgacag
     3361 gcaactgtca acttactgaa ttgtgagcaa catagcattt aacggggaac ctgttttctg
     3421 cagtaaaaag gcccaccgga gcggatgggc ctggaaggat agcggtcatg tgatgccggt
     3481 ttcccggtaa ctcagcaccg gtatctgagt caacgttttc tctactgggt catttccgat
     3541 acgccctgcc tgctgacagg ctttcatcac atctgaaaat atagcaccct gactgatact
     3601 gtagtaccta aggttccaga aactgtgatg tatccggcac agaaaagccc ctccggagag
     3661 ggctggagag tggcgctatg tgccattgca tgatgccggg tgcctcccgg tgaattcagt
     3721 accagcacct gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag
     3781 ggggattcac catgccagtt tcttttaaca aactccccgc aaaacagaca actgtcaacc
     3841 gtctgaattg tgagacattt aaaaaaaagg cccgcaaaag cgagccaggg aaaataagtg
     3901 tggcgcgttg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     3961 ggctgagcta acaacgcatg atgcagataa tggaccgcca tcggggactt gaaccccgcg
     4021 cagccagctt cgaaggctgg cgctctttcc tgatgagcta atggcggtat gtgatggtgg
     4081 cccttgctgg atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag
     4141 ctaaagggcc gggagccgca taataacgac gcgtaattaa ttcttcaata tcatccgttc
     4201 tggctgacta aatcctgtac ttcccgaacc gtctgctcaa aacgttcagt ctccagctca
     4261 acgccaattg cacgacgccc gagcgccagt gccgctttca ctgtcgaacc caaccccatg
     4321 aaaaaatctg caatcaggtc acccggacga ctgctagcgc tgattatctg ctgcagcatt
     4381 tctgccggtt tttcgcacgg atgtttcccg ggatagaact gcaccggttt atgcgtccac
     4441 acatcggtat acggcacctg caccgtcacg ccaaaatacc gccgcagatg tttatattca
     4501 ctcagcagct ccgcatactg ccggttcagt gaggtatacg tatccaccag ctggtggtgg
     4561 agcttttcca gttcaccacg ccgatgcttc tcttctgcca cccgggcaaa cagcgcctgt
     4621 aatttcagat aatcgctttc attcggcaac tgccactgac cggcactgaa ccagtgcgac
     4681 accatgtttt tctttcctgt ggcatctgca atctgttttg ccgttatccc cagggcagct
     4741 cgcgcatcac gaaagtaaga aatcagcggg gccatcacat gctgtttcag tgcactgccc
     4801 ttcgccgcat acccggcatc tttcggacga tacggcccct gataatgttc cgcgaacaga
     4861 atgcgctctg tggcggggaa atacgcccgc aggctttcct tgttgcatcc gttccagcgt
     4921 ccggacggct tcgcccagat aatatggttc agcacactga agcgttcacg catcatgatt
     4981 tcgatatcag atgccaggcg atgaccacag aacaggtaaa gacttccggc aggtttcagc
     5041 acccgccaga actgcgccag acactggtcc agccacttca ggtaatcatc gtcgcccttc
     5101 cactggttat cccagccctc aggcttcact ttaaagtacg gcgggtccgt gactatcagg
     5161 tcaacagaat tttcgggtaa cgaccggata aattccaggc agtcggcgtt gattaactca
     5221 caactggata tttttacagt attaagcatg gatcattaag cctgtctctg ataggctcat
     5281 tctgcttttg cgcaaagcag tgggcctgag gtttgcttgt gaacccaacg catgagcaga
     5341 tggctggtgg gtgcccctaa cacccaccag ccgcccattt accacaaata aaaaagcctt
     5401 cactgcggaa ggcgtctgta acaaccgaac tgatagtctg ccagacccgc cataaccagc
     5461 tgggtcagta ttaactggca gcgttcgcgt gaaaggtaag tattctgcgc tatctccccg
     5521 actgtcgccg gttcggtaac gcttaattca ttaaacacca ctctggcggt ttctgtcata
     5581 tcctgctgtt ttagcatgtc ttttcctttt ccggttaacg tgacacacca ataactcttg
     5641 tcgaaaaagc cagcaagctg aaagacaggt attcaccgcc accagcgcgt ttactgtact
     5701 gacgcgattt cagtcataaa aaacccgctc ggcggcgggt tgtagaaact cttctaacgt
     5761 caggcataaa acgcccatcg ttatgacgaa tttaccacag attccggaaa aatcaacctt
     5821 gttacctagt tacctttttt aactgccgct cagcccatgc ttcttcaata tcaaaccggg
     5881 tcaccagcgc atcataaaat ttcttaactg ttttttccca tgacgcgcgt gttatctggt
     5941 ttgtcacctc gcatatagca ttaaatgcct ccgttgatgg tagtctttca tagccacgac
     6001 caccacaacg ctggcagtct ctgataacag gcataccacg ttttaccgac tcttcacgat
     6061 gaatggcgac accacgccca cggcaatcct tacaggcggt ggaaacctca ccctttccgc
     6121 cacactccgg acaggcaact tttaccacct ccctgacttt tttccattct tcccagtaag
     6181 acggatacac acctttcgta cactttgccc ataccggcgg cttaccatcc ggatactgga
     6241 tcttgtttgt aaaaacctcg ctttcaataa atttttttcc gtgacagcag gggcactgtt
     6301 ttttgctcgc cgcgctacgg gcataatctt caaacgcata cgaagccata atacgcatca
     6361 ctgccggttt tatttctgcc gggagttttc ttaacgccgc cacgcgatca caccgactga
     6421 gtgcatattc tgtcagcaat tctgttgccc gctctctgtc attcatacta atgcccattt
     6481 tcccaaggaa cgcagaaacc cccatctcag cccgattctg tgtcatgccc tgcgcggcca
     6541 tcacatcagt gatactcagc gcatctttcg acgttgaggc cgatgcatca gtcaggccgg
     6601 gggattttgg ggagtagtat ttcggtaaat cttccagttt cattttttga cctgcccttc
     6661 aagcattatg ggataaatct tcacccccag acgtccacca gatactggct gagcacgaac
     6721 gatattgatt tcatcaaact gctcatcgtc cattagcaac cccgcatgcg tcagcgcatc
     6781 cagcggcgct ttcagaatat tgtccaggtc acggcggcgc ttatccggtg gttctgcaat
     6841 aatttttatt gccaaccttc cggacaggct taatttcagc cgctgctggc gaacaataag
     6901 cgccactgcc cggcgataac gctccccggc ttttgataca aaatatgtgc tgccacgacg
     6961 acgccagtaa gtgttcaccg tcggcgggta aggtaaaaca aattctatgc gctcggtcat
     7021 ttatgctttc cacttcagaa cacccgaatt tctcgcgtgc attaaaaaac gaatcagcaa
     7081 caacagctga ctgccgtgtt tctcttcaaa atcttttact ccggcatgta gttcgttacg
     7141 gcatttacgg cataacggaa taacaaacaa atcatcagcc tttgttccca tccctcccag
     7201 tccatgacca atgatgtgat gcggatcatc tgcctgattg ccacacgtca tgcatttctg
     7261 cgtttttacc cagcgcgtgt atacaggcat ctcttcccgt tgtgatttct ggcgctggag
     7321 atactgagcc ggtgactccg gatcaacggc aatgctgacc accgtctttt cctgtggtgg
     7381 gttctgttgc tggtggacgt gaggcagtaa cgcaatattt tttgtgcgct gcttcagtat
     7441 gctggtggct gtctgctctc ccggtatgat gtcgctttca ctgtacacag agcgaatttt
     7501 ttccgcacgc aaccccagcg aacgacgtaa taccgcttcc ggtagcgcgt ccgccacctg
     7561 attgcggacc gcccaccagg ataattcagc cagagataat tcacgctcct gcgtaccgct
     7621 tattgcgtga ccgatgacgt caatcatcca tgctgacagg ttttgatgag caagttgctc
     7681 gagtgattcg gatgtctggt cacgcagctg gttgtcgcag tgccagcaca acaccattgc
     7741 gccggtacca taacggtgaa tgacggtttc gctgtgatga taatcgccgt gtggccactg
     7801 gcaggattta atatggcgca acagccagtc agacaatgca ccagcaccac cagcagcacg
     7861 aatcacccgt gcgttactga aaaacggcag caatgttttg tcttccacca gcggctggcg
     7921 agcggcagga acgaccccgg acggcagatt acgcatgctt tttggttccg gctccaccag
     7981 tattctgccg ttatggaatg ctgacattga ttcacggcct ggcttaacga taaccagacc
     8041 gagttccggt accagaacag gtcgaagtaa tacccgcaca ttacctccag atccgttgct
     8101 ggaatgtgcg ggacggacgc gttggccgtt cggagtaagg gagcctgacg gagattatcc
     8161 agtgacggta gtcgaggcta agggcttttt taacctcgca tccgcgcctg cggtaacact
     8221 gaatgagcca ttcggcctgt tcttcagtgc atgggggatg ctggtaccaa tcagatttaa
     8281 atgcgtgaga gcgccgcccg tgcctgctgg caaagacggc tgaattatca gaattgtgta
     8341 gtctggaatt ttgcgccatc ggctttctcc ggtggcacag tgttactcaa caggggttca
     8401 gccctgcgct gaattgtaga tgaattcact catcttcaaa agcagaaaaa ccagccttaa
     8461 tcccagcttc tttcagagac ggcaacgatg tgacaaattc atttgcacgc gaaataaaac
     8521 catccgtcac aagcccatcc acaaaatgaa ttaacgcagc tccactcttc ctttgttgag
     8581 actgtaaaca tttaatacgg cagtggctga caatagcgcc attctcaacg cgcacagtat
     8641 agaggccatc ttcactaaaa atttcacgta attcttcgat tttcatcaac agaatccttc
     8701 cagataaata gcactcccct gttcggggtc catccctctt ctccctgcgc gctacttaag
     8761 tatttttgat tctattccgg caccatctaa aacttcaaac gcgttgaaaa taaaaacaaa
     8821 aacccgccga agcgggttaa gtgtgggtgc attgaggatg cctgacccat cagaggtggc
     8881 gagggatttc tcccccgcct ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
     8941 acctccgtct ggccggttcg aattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
     9001 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
     9061 atagcctgct tctccttgcc tttcggcacg taagaggcta acctagattt gccgttcata
     9121 gattgagcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
     9181 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgctgcaat
     9241 tctacttaac tctccttttc ccgcaaaccg tttttatccc cagcggcaaa tcgaatacac
     9301 caccagcgcc accgccatcg caattcctac cgttgttaat gcttcaggcc aggtcatcgt
     9361 aaaatatcct ccacgcttat cagtccgttc cgctccagat aactcatcgc cttatccggt
     9421 aatttgcagt ctggcttcgc ttttctcagt tgccaggtta actgctttac cagcatggtt
     9481 aactcatcga ccagacgctg atatcccact ggtttgtatt catgcaattt accggctggc
     9541 tctgctgcca gcgataccag tgcgatttcc agaacagcaa tatccatctt atatgtgcgg
     9601 atgatgtcat ggtcgattgt gcccggtatg cacagtctct gtgcttcaat agtctcctct
     9661 gcgtgagcta ttaactgctc tctggtaaaa gtcgtcatgc cgtagcccct tcttgatatt
     9721 tttcaaacca gaacacaacc ggctctgctt ccagcgatgc cagcgcaatc cgtgccagtt
     9781 ccatttgttc accacgggta agcccgtttt caagcgggtt tttaatgaac aattcaatac
     9841 gttctttggt aatagtggtc atgtgttact ccttaacccg cagtgctttc aactgatgag
     9901 gggaacaaaa tcttttcatc aaaccctgca ttcatatcat gaacagcaac acaccaatcc
     9961 atcgacgaac gattatcaag agcctccatg atttcatcca tgcggcgtag gtcatacagg
    10021 taaatgcttt tatcgccaat ggtgtaaaag ccaatttttt tcggtgat
//
LOCUS       DAQSWQ010000142         9674 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.144, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000142 DAQSWQ010000000
VERSION     DAQSWQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.144"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..1710
                     /locus_tag="R8G18_003765"
     CDS             <1..1710
                     /locus_tag="R8G18_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEE9797358.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="R8G18_003766"
     CDS             1711..3360
                     /locus_tag="R8G18_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HEE9797359.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="R8G18_003767"
     CDS             3365..4141
                     /locus_tag="R8G18_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HEE9797360.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="R8G18_003768"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="R8G18_003768"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEE9797361.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="R8G18_003769"
     CDS             complement(5430..6752)
                     /locus_tag="R8G18_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEE9797362.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="R8G18_003770"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="R8G18_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HEE9797363.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="R8G18_003771"
                     /pseudo
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="R8G18_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="R8G18_003772"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="R8G18_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HEE9797364.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2355 a         2484 c         2605 g         2230 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt acagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaa
//
LOCUS       DAQSWQ010000143         9648 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.145, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000143 DAQSWQ010000000
VERSION     DAQSWQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.145"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..670)
                     /locus_tag="R8G18_003773"
     CDS             complement(<1..670)
                     /locus_tag="R8G18_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEE9797365.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDS"
     gene            complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="R8G18_003774"
     CDS             complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="R8G18_003774"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEE9797366.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5045..5650
                     /gene="azoR"
                     /locus_tag="R8G18_003775"
     CDS             5045..5650
                     /gene="azoR"
                     /locus_tag="R8G18_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEE9797367.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5701..7017)
                     /locus_tag="R8G18_003776"
     CDS             complement(5701..7017)
                     /locus_tag="R8G18_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HEE9797368.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7007..8764)
                     /locus_tag="R8G18_003777"
     CDS             complement(7007..8764)
                     /locus_tag="R8G18_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HEE9797369.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8780..>9648)
                     /locus_tag="R8G18_003778"
     CDS             complement(8780..>9648)
                     /locus_tag="R8G18_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEE9797370.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2630 c         2274 g         2296 t
ORIGIN      
        1 cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag tgagagataa cgctcaaccg
       61 gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcgttattt cctaactgag
      121 gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtcccca gtcatacggc
      181 ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg aacaacgata gccgtatgaa
      241 ctcgttctac ggcctgattc aatagcgcga tgctaaagcg tgcgttttca ccgcagtttg
      301 ttgactggtc ttcaatccag attttttcat gcggaatgtg ccagaactga tgagcgatat
      361 ccgcctggat ggtcgcttct gctctgccag tggtgcggat agtgttgtag tgcggatgct
      421 gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat accaccacta atcagtaaag
      481 gaatttgctg atcgcgagca atcttacatg ccgcgtcgat agtcggcata accgcattgc
      541 ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc ggagaaatca tcctgcgcca
      601 gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc ggaaacgggg
      661 taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat aatagtgaga
      721 agcggagcac tctgaaagga ggaagtcgta tttccttaac cgccactccg caagcctgac
      781 atatctttta aggaaatgct gtagataaac attattcatc ttgcagcaga gaattgtatt
      841 tttctgaatt cctgaatttt tacccttacc gttttagaat taataaaaaa cccggcgcta
      901 aatgcttacc gggttttttc tttatcaggc aaatagcagg gttaaccgct aatctgctcc
      961 atcgcctgca aaatacgctt atctgaaatc ggataaggcg taccaagttg ttgagcgaag
     1021 taactaacac gcaactcttc tatcatccaa cggatctctt tcacgtcttc atcctcacga
     1081 cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct gctggacgtt ttcgactttc
     1141 agcatctgag cacggtcgcg atgtggatca accgccagtt tttccagccg tttttcaatc
     1201 gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg
     1261 cgatatacca gcccgcccat ctgcgcttta atgtcagaaa gccccagcgc catcgtcata
     1321 tccacccgcc ccttcagacg tttgttgata ttgaacactg ccgtaaggat ttgctcgacc
     1381 tgcttcgcaa tatccaccac cgtgtcgttc agttcggcac gcactttttc atgcagcgca
     1441 gcaaagcctt cttccgtcca gaccgggcca ccattggcgt cgatcaattt atccacaccg
     1501 caggagatac aatcgtcgat cagctccagc actttgccat acgggttaaa gtacagcccc
     1561 agcttggctt tgtttggtaa cttctcatgt aaatacttga tcggcgaggg aatattcagc
     1621 agcagtagac ggcgaagacc gttccacatt acctgttttt gctccagcgg attatcaaac
     1681 agtttgatcg ccacactgtc gcgttcatcc actagcgctg gccacgcctt cactttgtag
     1741 ttgccacgct tctgttcgta gctttccggc agctgaccaa aactccagat atgcagtccg
     1801 ctctgctcga taccgtcatc cgccaccgca gacagcgttt cctgcacttt gcctttcagc
     1861 gcatctttca gatcttgtag cgagcgccct tcttttagct tcttgttttt gtcatccacc
     1921 acgcggaagg tgattttcag gtgatcgggc acctgatccc agtgccagtc ttcgcggtca
     1981 acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc
     2041 agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg gtgcaggcac aaaattacgg
     2101 cgtaccggtt tcggcaacga tttaatcaga gcaatcacca attcgcggcg cagaccgggg
     2161 atctgccatt caaacccgct ttcctcaacc tggttaagta acggcagcgg aatatgtacg
     2221 gtcacaccgt ccgcgtccgc gcctggctca aactgatagc tcaaacgcag cttgagattg
     2281 ccctgatgcc agaagttcgg gtaatccagc tttctgattt tttccgcccc ttctttgatc
     2341 aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc ggctgacttt tttccaccag
     2401 ctatcgaagt gacgagcgga gattacatcg tggctgatgc gctggtcgta gaactcaaac
     2461 agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt tgtgttccag ctcttccact
     2521 tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc
     2581 accagagcgt ggcgaataaa gagttcacga cataacgccg aatcgatctg gctgtagttg
     2641 accttgcgcg cagcaacaat cggcaaacca taaacagtga ctttttccgt tgccatcacc
     2701 gcgccctgcg cccgttccca gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca
     2761 acgggttcta cccattccgg gtcgatacgc gcagcaatgc gcccccacag gcggctggtt
     2821 tctaccagtt ccgccaccat tacccatttc ggcggctttt tgaataaacc agaaccgggg
     2881 aagatggaga aacgcgcgtt acgtgcaccg gtatattcct gtttatcggc atctttcatg
     2941 ccgatatggg aaagcaaacc ggtcagtaag gcgatgtgaa tttcacgata ctccgccggt
     3001 tcgctgttga ccggaatgcc aagttctttc accacctggc gcaactgggt gtagatatcc
     3061 tgccattcgc gcacgcgcag atagttgagg taatcggtac gacacaggcg acggaaggcg
     3121 ttggaagaaa gcgccttttg ctgctcgcca agataattcc acagatttac aaacgcgagg
     3181 aagtcggatt ctttgtcgtg gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc
     3241 atcggacgtt cgcgcggatc ctgaatggag agcgcggacg tgataatcat cgcctcacgc
     3301 acgcagccat gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc gacaggcaac
     3361 tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc tggcctgttc atccgtggtg
     3421 atcgcgccca gttcttcgag cagacgcacg ccatcctgga tattgcgttt atccggtgct
     3481 tcgacaaacg ggaacgcagc gatatcgccc agccccagcg cggtcatctg caaaataacc
     3541 gaggccaggt tggtacgcag aatctccgga tcggtaaact ccgggcgcga gaggaaatcg
     3601 tcttcggaat agagacgaat acagatccct tcggacacac gaccacagcg gcctttacgc
     3661 tgattggcag acgcctgcga aatgggttca atcggcaaac gctgcacttt ggtgcgatag
     3721 ctgtagcggc tgatacgcgc tgtaccgggg tcgatgacgt atttaatccc cggtacggtc
     3781 agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc gtccgctgtg cgactggaac
     3841 acgcggttct gttcgctgtt cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt
     3901 aagttcagct tgttcagcgc atcggcggta tcgcgaattt cccgctcacc actcataaag
     3961 atcagaatgt cgccagggct ttcctgactc agttcgtcta cggcgtcaaa aatcgcctgc
     4021 aactgatcgc gctcggtgtc atcggcttct tcaacaatcg gtcgatagcg cacttccacc
     4081 ggataggtcc gaccggagac ttcgataatc ggcgcattat taaagtggcg cgaaaagcgt
     4141 tccgggtcga tagtcgcgga agtgataatg atttttaggt caggacgccg cggcagcaac
     4201 tctttcaaat agccgagcag aaaatcgata ttcaggctgc gttcgtgcgc ttcgtcaata
     4261 atgatggtgt cgtactgcat cagcaggcgg tcttgctgga tctccgccag caggataccg
     4321 tcggtcatca gcttgaccat cgtgttatca cttacgtgat cgctgaaacg cactttgtaa
     4381 ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa tacggttcgc caccgttctt
     4441 gccgccaggc gacgcggctg ggtatggccg atcagccctt taatcccgcg ccccagctcc
     4501 atacagattt tcggtaactg agtcgtttta ccagaacccg tttccccggc gacgatcacc
     4561 acctggtgat cacgaatcgc ttcgagaatg tcctgttttt tctgactaac cggtaaattg
     4621 tcaggataag taatttccgg tcgtgccgct tcacgcagca ggactttccc tgccgcctgg
     4681 tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg catcaggatt tttaaccttc
     4741 ttcacgccgt gcagacggcg agaaaaacgc agtctgtcac gcagcatcag cgaatccagc
     4801 cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg tcataacgtt agtgggcagt
     4861 agtctgcctg attaattttc tttgaaatga tagatataga gtaccacatc acggctttct
     4921 atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct ctgtggcagg
     4981 attgtgaata aagtgtcaac aagcaacggg gcatcgccca ttcaaacatc tataaggaaa
     5041 caccatgagc aaggtattag ttcttaaatc cagcatcctg gcagggtact ctcagtctaa
     5101 tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac
     5161 cgttcgcgac ctggctgcaa atccgattcc ggtactggat ggcgaactgg ttggtgcact
     5221 gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa gctctggcgc tttccgatga
     5281 gctgattgcc gagctgaaag cccacgacgt tatcgttatt gcggcaccga tgtataactt
     5341 caacatctca actcagttga aaaactattt tgacctggtt gcacgcgcag gcgttacttt
     5401 ccgctatacc gagaacggtc cggaaggtct ggtaacgggt aaaaaagcca tcgttattac
     5461 cagccgcggt gggatccaca aagatggacc aacggacctg gtgacgccgt atctgtccac
     5521 gttcctcggc tttatcggca ttactgatgt gaaatttgtc ttcgccgaag ggatcgcata
     5581 cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa gcagccatcg acagcattgt
     5641 tgctgcataa ccggacacga tcttgatgtg gactgtagtc cagtccacat cccaatccac
     5701 ttacctgttt tcccataatt tcagcatcgc tttgtgatct tccttaagca caatatgcga
     5761 gcgtctggct cgaataaagc taatcgcttc atcaacggtt ttacagtatc cgtaacataa
     5821 cagccatgcc gccactacca gcgcactgcg cgataatccc agtgcgcaat ggaccagaac
     5881 gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga gctccccctc
     5941 ttccggaacc accagatcta gcatcggtac acagaaatag agtcgatctt ttgtcgctcg
     6001 tccacgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg
     6061 aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc gacgacaaaa
     6121 ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt aaacggcggg
     6181 cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac cgtacccacg
     6241 gccaataatg agtagcgata atactggcca acataaccag actgaccacc ataactgaac
     6301 catcatcatt agctccatca acacaatgca cgcgcacgcg cctacgacat atggcagtgc
     6361 tattttgatt cgacgttgat caggtttctg ataattccac cgacggtcga cgggcaccat
     6421 ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat gatgctgcca
     6481 tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccgcagactt tacgccacct
     6541 cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa tatgcagcga
     6601 aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc atcccgtcac
     6661 cccactcact tcaggacgga taaaactaaa cttcagcgga tagagcaaaa aaccgcagca
     6721 ggccattacc gttgccagaa taagccggtg gacaagtcgg cgctgttcga atgtcgtgct
     6781 acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa caatagtcca
     6841 gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctgggaatat catggctatt
     6901 actgtcctga accgcggtga actgattaag agatccatag gtgaagaaga aaaacggggc
     6961 cagcaacaat aaccagccag ctccttgtag caatacgttc cggcgttcag ttatcacgac
     7021 gcaccgccat cgaaaccgta aaaatgcccc actcatcaat ccgttgtgtg catttatcaa
     7081 atccagcatc atgcacgagc aaatccatct ccccttgcgt acgcacgcgc attacccaca
     7141 gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt gggtgccacg
     7201 gctgcccggt gtagatgaga atgccacccg gttcgatggc attggcaaga cctgcgagtg
     7261 agttttttac ctgctcattt tcgggaaaca gctcatacag tccagagaca atcgccagcg
     7321 taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc tgttcaaaac
     7381 gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca acatttaact
     7441 cactgtaatc acgtaataaa atatcgctta cggcaggctc gtttgccagt gcatccagta
     7501 catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct ttgacgtgga
     7561 gatcggcaac ggcttgttta atcagtattt gtaaatgagt tttgcgctgg cgaataccgc
     7621 gccagccaac actgttcagg tagattttgt cgattaagcg cccgaatgcg ttactgcctt
     7681 gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagcca gtgctcattc
     7741 cgagatgcag gcccgcagag tgcgtcccga acaatttcat cgctttacgc ataaagcgat
     7801 acgccaaatc aaccggcgat aatggcacgg gtccacctga aaggaggcgc cagcgatccg
     7861 ctgatggtcc agtgcggtct tcatgctgat aatcaaattt ttgtgacttg ttagcatata
     7921 aacgacgaat aaagctttgc attttttcaa atgcctgcgc cctgttctct tcacccaacg
     7981 tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt aatctctgat
     8041 aaaaatcaat ctgcggttgg cgatgcacca catagtcatc gcctgatatc agaagttgcg
     8101 tggggagcgt aatcgccgcc gcatctctaa taatacgttc agacgtttta tagagatcga
     8161 gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa ctcgccaccc
     8221 gttgccgatc gtgggtcaaa tagcgtcttt tcacatagga attaataaaa aacagaccac
     8281 gcagacgatg ccatagcgtc agcgcaggac gtgccagcgg cacatacaat ttaaccttaa
     8341 aggccggaga agccagcacc agcccgcgaa ttgcaggcgc ataatcatga acccatgtgg
     8401 caaccagcac tgcgccgacg ctttgcgcga tcacaaccac ctcttccagt ccaacctggc
     8461 tgtcgctggc agcaaaacgg acaaattcat cgacatcctg cactgaacgc gcaagagatg
     8521 ggctataacc acgcggcccc gaagtttttc catgcccccg ggcatcccat gcataaaaag
     8581 cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa tgttcatggc
     8641 cacgatgaaa taagacgatg acctttttcg cccctggctg taaagcgggc cagtgccgat
     8701 aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagagatg cgtgaatttt
     8761 ccattttctc tattttcctt cagtaacagc agtaacgaat aaaataaaaa aataccggag
     8821 cggtgaaaat taatgagtca atcctgtcaa ggattccgcc atgtccaggt aatagtttcc
     8881 cgctatcttt aacaccaaca tctcgtttga tggctgacat cactacatcg ccgcaaaaac
     8941 cactaatacc aattaacaaa cccgctaata ttgcctgtaa tgtatttagc ggtgtcagta
     9001 acggtccaat gatcatcgac gctatcatgg tggtgattac gccgccgacc agaccttcca
     9061 gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat ttcccccata
     9121 aatactgtgc aatatcgttt gacgcggtta atgccaaaag aaataacacc agtaaggccc
     9181 cgccctggat attcatggat ggcaacatta ataaccaggc ggcatgactc aaggcgaaaa
     9241 cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg gaggggtcac
     9301 ccacaaaaac tcgccaggtg gctaatatca gaaacccagc caaaggtata aataacagaa
     9361 acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc caatataacc
     9421 accgcggaaa atgtacggat ataagcatac aatattcttt caatgccaga aaacttatca
     9481 gcgcaaaaaa cgccaacgtc atccagcgtg gacctgaaat caccagtgaa aaacaaatga
     9541 cgatgcccca ccaggtacga atacgtaatg taacctcacc cccttttctc tccggtaacc
     9601 gccatagcag aaaacggttt atcagcgttg ctaaaattaa caacgcga
//
LOCUS       DAQSWQ010000144         9621 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.146, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000144 DAQSWQ010000000
VERSION     DAQSWQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.146"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            16..549
                     /locus_tag="R8G18_003779"
     CDS             16..549
                     /locus_tag="R8G18_003779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797371.1"
                     /translation="MIEGPADFNDRVDELFLAHQLPVAIYSYCQYQDGAAPGRGAWTP
                     FAEFSPEWQALQAARRIQAQTYFIDLPCWAQSEEVDDSPDTQEESQALLLRATRMDNS
                     DTLWDHLFEDESQQTALPSALAHYFAQLRGDSPGDALNRQREAFMARWIGWAMQQNNG
                     DVLVVCGGWHAPALAKM"
     gene            669..2195
                     /locus_tag="R8G18_003780"
     CDS             669..2195
                     /locus_tag="R8G18_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797372.1"
                     /translation="MPAPVWQSWYWQWGLQQAGEQLLKTVLTRLRQHKLPASTADMAA
                     AHLHAMALAQLRGHKLPLRTDWLDAIAGSLIKEALNAPLPWSYRGVIHPDTDPILLTL
                     IDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAELTLNRFNPNGLAQSQVLHR
                     LAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAALIEAACFGATLQEAARHKLE
                     ADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLIAQENQFAEMGQALEVLYAL
                     WRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEKEFHAHLHSWQALCHILRDL
                     HSGVNLPGVFLSAAVALLERRSQAIHVPALDRGAALGALMRLEHPNASAEAALTMLAQ
                     LSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLSDDDFINALPDLRAAMAWLP
                     PRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHHQQLEQQALASLQNWGVFHV
                     "
     gene            2188..3324
                     /locus_tag="R8G18_003781"
     CDS             2188..3324
                     /locus_tag="R8G18_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEE9797373.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3321..5321
                     /locus_tag="R8G18_003782"
     CDS             3321..5321
                     /locus_tag="R8G18_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEE9797374.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5446..5907
                     /locus_tag="R8G18_003783"
     CDS             5446..5907
                     /locus_tag="R8G18_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HEE9797375.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5948..6418)
                     /locus_tag="R8G18_003784"
     CDS             complement(5948..6418)
                     /locus_tag="R8G18_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEE9797376.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6465..7184)
                     /gene="yehT"
                     /locus_tag="R8G18_003785"
     CDS             complement(6465..7184)
                     /gene="yehT"
                     /locus_tag="R8G18_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HEE9797377.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7181..8866)
                     /gene="btsS"
                     /locus_tag="R8G18_003786"
     CDS             complement(7181..8866)
                     /gene="btsS"
                     /locus_tag="R8G18_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HEE9797378.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9088..9153
                     /locus_tag="R8G18_003787"
                     /pseudo
     CDS             9088..9153
                     /locus_tag="R8G18_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            9381..>9621
                     /locus_tag="R8G18_003788"
     CDS             9381..>9621
                     /locus_tag="R8G18_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HEE9797379.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT         2355 a         2530 c         2468 g         2268 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacccggtat gcctgccccg gtctggcaaa gctggtactg gcagtggggc ttacagcagg
      721 cgggagaaca actgctaaaa acggttctta cccgtttgcg ccagcacaaa ttgcctgctt
      781 cgaccgcgga tatggctgcc gctcatctgc atgcgatggc gctggcacag ttgcgcggac
      841 ataaactacc gttacgcact gactggctgg atgccatagc aggctcgctg attaaagaag
      901 ccctgaacgc gccgttgccg tggagctatc gcggagttat tcatcccgac accgatccga
      961 ttctgctaac gttgatagac acattagcgg gtgacggatt cggtaaactt gccccttcta
     1021 cgccacaacc gcctctgcca aaagatgtca cctgcgaact ggaacgtacc gcaatctctc
     1081 ttccggcgga gcttacctta aatcgcttta accccaatgg gctagcgcaa agtcaggtgt
     1141 tacatcggct ggcaatactg gagatcccag ggattgtacg ccagcaggga agcacactga
     1201 cacttgcagg caacggtgaa gaacgctgga aattaacccg cccgcttagc cagcatgcgg
     1261 cattgattga ggccgcctgc tttggtgcca cactccagga agccgcacgc cataaattag
     1321 aagccgatat gctggacgcg ggtggaatcg gcagtatcac cacatgtctt agccaggcgg
     1381 cgttagcggg tctggcgtcc ttcagtcaac aattactgga gcaactcaca ttattaatcg
     1441 cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga agtgctatat gccttatggc
     1501 ggctggatga aattagcggt atgcaaggcg cgcagatatt acaaacgacg ttatgcgcgg
     1561 ctatcgatcg cacgctgtgg ctgtgtgaat ctaacggcag accagatgaa aaggagtttc
     1621 acgctcacct gcatagctgg caggcgcttt gccatatcct gcgcgatcta catagcggcg
     1681 ttaatttacc cggcgttttt ctttctgcgg cggtagcctt actggagcgc cgcagtcagg
     1741 cgattcatgt cccggcgctg gatcgcggcg cggctcttgg cgcactgatg cgtctggaac
     1801 atcccaacgc cagtgccgaa gcggcgctga cgatgctggc gcagttatcc ccggcacaat
     1861 ccggcgaggc gctgcacggt ttgctggcgc tcgcccgtca tcaactggcc tgccaaccag
     1921 catttatcgc cggtttcagt agtcatttaa atcaactgag tgacgacgat tttattaacg
     1981 ccctgcccga tttgcgcgcg gcgatggcct ggctaccgcc acgagaacgc gggacactgg
     2041 cgcatcaggt gcttgagcat taccagcttg cgcagcttcc tgtttcagcg ctgcaaatgc
     2101 cgttgcattg tcctccgcaa gccattgcac atcatcaaca actcgaacag caggcactgg
     2161 catcgctgca aaactgggga gttttccatg tctgaactga acgatcttct gaccacccgt
     2221 gagctacaac gctggcgatt aattcttggc gaagcggcag aaacgacgct ttgtgggctg
     2281 gatgacaacg cccggcagat agaccacgcg ctggaatggc tgtatgggcg cgatcctgaa
     2341 cggctccagc gtggtgaacg ctccggtgga ttaggtggct caaatctcac cactcctgag
     2401 tggatcaaca gtattcacac gctgtttccg caacaggtga ttgagcggct ggaaagcgat
     2461 gccgtactgc gctacggcat tgaagatgtg gtgacgaatc tcgacatgct ggaacgtatg
     2521 cagccttctg aaagcctgct acgcgccgtt ttgcacacca aacatctgat gaatcccgaa
     2581 gtactggctg ccgcccgccg gatagtgcgc caggttgttg aagaaattat ggctcgactg
     2641 gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc gccgtcgccg ctcatttatt
     2701 ccactggcgc gaaactttga tttcaaaagt actctgcgcg ctaacctgca acactggcac
     2761 ccgcaacacg gcaagttgta tatcgaatcc ccccgcttta acagccgcat taaacgccaa
     2821 agcgaacaat ggcaactggt cttactggtt gatcaaagcg gatcgatggt ggactcggtg
     2881 atccactctg cggtgatggc ggcctgtttg tggcagttac ccggcattcg tacccatctg
     2941 gtggcgtttg acacaagcgt ggttgatctc acggcagacg ttgccgatcc ggtagagtta
     3001 ttaatgaaag tacagttggg cggcgggacc aatatcgcca gtgccgtgga gtatggtcgg
     3061 caacttattg aacaaccagc aaaaagcgtc attatcctcg tgagcgattt ttacgaaggg
     3121 ggttcatcat cattgctgac gcatcaggtg aaaaagtgtg tccagagcgg catcaaagtg
     3181 ctgggactgg ctgcgctcga tagcaccgca acaccttgct atgaccgcga tacggcccag
     3241 gcgctggtta atgtcggcgc acaaatagcc gccatgacgc cgggcgagct ggcatcatgg
     3301 cttgcggaga atcttcagtc atgaattcac tacgtccgga attattagaa ctgacaccgc
     3361 aggccctgac ggcgttaagc aatgccggtt ttgttaagcg cagtcttaag gaactggaaa
     3421 atggcaacgt cccggagatc agccatgaga acgacgcttt aatcgccacc ttcagtgacg
     3481 gtgtccgtac ccagctggcg aacggccagg cactgaaaga ggctcagtgc agttgcgggg
     3541 ccaacggtat gtgccgtcat cgcgtgatgc tggtgttaag ttatcaacga ctttgtgcca
     3601 ccactcagtc tacggaaaaa gaagaagagt gggatccggc aatctggctg gaagaactgg
     3661 ctacccttcc cgatgctacc cgcaaacgcg cacaggcgct ggtcgctaaa ggcatcacca
     3721 ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg cttaccgatg agcgatatgc
     3781 gtttttattc ccgcagcagt attcgtttcg cccgttgtga ttgtattgaa agcacacttt
     3841 gcgaacatgt cgtactggca gtacaggcct ttgtccaggc aaaagcgcag caagcggaat
     3901 tgactcattt aatctggcag atgcgcagcg aacacgtcac atcatctgac gatccgtttg
     3961 ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca actaagccag gcattatggc
     4021 tgagcggcat cagccagccg cttattcact acgaggccgc tttcagccgc gcgcagcaag
     4081 cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct acggcaactg cgcgccagcg
     4141 ttgatgcctt ccacgcccgc gccagccact atcatgccgg agaatgctta cgtcagcttg
     4201 cggcattaaa cagtcgatta aattgcgcac aagagatggc ccggcgcgac agtattggtg
     4261 aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg cattgccgga gaagcaaagc
     4321 ttgatcatct gcggctggtg tctttaggta tgcgttgctg gcaggatatt gagcattatg
     4381 gtttacgcat ctggtttacc gatcccgaca ccggcagtat tttgcacctt tcgcgcagtt
     4441 ggccgcgaag tgaacaggaa aactcaccgg cagctacgcg tcggctgttt agttttcagg
     4501 ctggcgcact agcgggcggg caaattgttt cacaagcagc aaaacgcagt gccgatggcg
     4561 agctgctgtt agctacccgc aaccgcttaa gcagcgttgt gccgctgtcg cctgatgcct
     4621 ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt ggctttgcgg gaatatttac
     4681 gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt cgataactta tttattctgc
     4741 cggtcgctga gtgtatttcg ctcggttggg atagcagccg ccagacgctg gatgcgcagg
     4801 taatcagcgg cgaaggtgaa gataatctcc tgacgttatc ccttccggca tctgcctgtt
     4861 ctccttttgc cgttgaacgc atggcggcgc ttttgcaaca aacagacgac cccgtgagtc
     4921 tggtttctgg ctttgtcagc tttgtagagg ggcaattgac actggaacca cgggtgatga
     4981 tgacaaaaac ccgcgcctgg gcgctggacg cagaaactgc gcctgtggca ccgctacctt
     5041 ctgccagcgt tttgcctgtg ccgtctaccg ctcatcagtt gctgatgcgc tgccaggcgt
     5101 tacttattca actgctccat aacggctggc gctatcagga acagagcgct attagtcagg
     5161 cagagttgct ggcgaatgac ctctccgcgg tcggttttta tcggctggca catgtgttgg
     5221 gacaatttcg taatacagaa agcgaggcac gggtagaagc aatgaataac ggtgttttac
     5281 tttgcgaaca attattcccc atgcttcagc aacaaggatg aaatagtgct ttttactaag
     5341 agttctactc cagttccgga ctgctcacgc cacggtatta ggcatatcct atatagcccc
     5401 tggtgagagt caccagttcc ttgattaaat aaaatggagt tttacatgaa ggctttcaat
     5461 aagctgtttt ccctcgttgt tgcatctgtt ctggttttct ctcttgctgg ctgcggtgac
     5521 aaagaagaat cgaagaaatt cagcgccaat ctgaacggca ctgaaattgc cattacctat
     5581 gtctacaaag gtgacaaggt gcttaagcaa tcttctgaaa ccaaaattca atttgcctcc
     5641 attggtgcaa ccaccaaaga agatgctgcc aagacacttg agccgttaag cgccaaatac
     5701 aaaaacatcg cgggtgttga agaaaaatta acctataccg atacctacgc gcaggaaaac
     5761 gtgactatcg atatggaaaa agtggatttt aaagccctgc agggtatttc aggaatcaac
     5821 gtttctgctg aagatgccaa aaaaggtatc actatggcgc aaatggaact ggtgatgaaa
     5881 gccgctggtt ttaaagaagt gaaataatct gtcggcggcc atgtttcgca tggccgccat
     5941 acccgtctta gcttttcttc acatgctggc gcgctgccag tccacgcaga aaataacgta
     6001 aaaattgatc gccgcattcg cggaagtttt tatgatccgg tgcgcgcatc atcgctgtaa
     6061 tttccggcat cgaaacgcgg aactgctgtt cggtgaggat cgccagaatg tcatcggttt
     6121 tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg cgacgttccg
     6181 gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg ccattgagga
     6241 atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct ttacgtagcc
     6301 agacggcgat ctgttccgcg gtggcttcga cattacccag cgccagaata cgcaccaggt
     6361 cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta cttagcatga
     6421 ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag gccaatcgcc
     6481 tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt acgcaaaatc
     6541 aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag gttaaccaga
     6601 tactgacgat ggcagcgcag tagtggtgta cgactttcca gggtacgtaa tgtcaattcg
     6661 gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg actgctgaca
     6721 aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca agggataaat
     6781 ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg ctcctgacgc
     6841 aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag ataatcaaag
     6901 gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac
     6961 gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg cggcatctgg
     7021 atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg
     7081 tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatac acgcaggttc
     7141 tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg tccctccatg
     7201 gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt attccatagt
     7261 catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc ccactggcat
     7321 tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa tgttgcccct
     7381 cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc ccatgtttaa
     7441 tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg gataattctt
     7501 gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca atttgcagat
     7561 aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt
     7621 ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg ctgtcgcggc
     7681 ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc acctgggcgt
     7741 gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat tgcccggcaa
     7801 gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt attgaactga
     7861 ataaacggtt tttggcttca tacaatttga tggtgcccat cacgcgctga ttttcgccac
     7921 gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag caacgataag
     7981 gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt aaggtgtaag
     8041 gcgaagaaat cggtttgcca ggtaaatggt ggtcgtcacc aattccggta aaggccagca
     8101 atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac agcacctgag
     8161 ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc gtcgaggctg
     8221 ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat
     8281 cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc attggcgcag
     8341 caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgcc aggatgatca
     8401 gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg ttaaagactt
     8461 tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc aatccttcaa
     8521 cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat cgatgtaagc
     8581 cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg cccattaccg
     8641 cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac caggtgccca
     8701 tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga cgaaccgtga
     8761 cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg actaaaaaaa
     8821 cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc gcaaaccaca
     8881 cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga actcttgcag
     8941 aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata aaatcaccct
     9001 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
     9061 cgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
     9121 ctttgtgata ttaaccctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
     9181 agatcgtcca ggagcgccgt ttttcaaggg ttgaatagat gattcgttta ccatgtcgac
     9241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
     9301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
     9361 cacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
     9421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
     9481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
     9541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
     9601 agtctgctga cgagtggttt g
//
LOCUS       DAQSWQ010000145         9499 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.147, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000145 DAQSWQ010000000
VERSION     DAQSWQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.147"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(149..1231)
                     /locus_tag="R8G18_003789"
     CDS             complement(149..1231)
                     /locus_tag="R8G18_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEE9797380.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="R8G18_003790"
     CDS             complement(1228..3024)
                     /locus_tag="R8G18_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEE9797381.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="R8G18_003791"
     CDS             complement(3275..3631)
                     /locus_tag="R8G18_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797382.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="R8G18_003792"
     CDS             complement(3689..4111)
                     /locus_tag="R8G18_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HEE9797383.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="R8G18_003793"
     CDS             complement(4127..4852)
                     /locus_tag="R8G18_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HEE9797384.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="R8G18_003794"
     CDS             complement(4874..5620)
                     /locus_tag="R8G18_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEE9797385.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="R8G18_003795"
     CDS             complement(5627..6691)
                     /locus_tag="R8G18_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HEE9797386.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="R8G18_003796"
     CDS             complement(6763..7188)
                     /locus_tag="R8G18_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEE9797387.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="R8G18_003797"
     CDS             complement(7185..7448)
                     /locus_tag="R8G18_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9797388.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="R8G18_003798"
     CDS             7560..8060
                     /locus_tag="R8G18_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEE9797389.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="R8G18_003799"
     CDS             8080..8274
                     /locus_tag="R8G18_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEE9797390.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="R8G18_003800"
     CDS             8274..8564
                     /locus_tag="R8G18_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEE9797391.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8843..8995
                     /locus_tag="R8G18_003801"
     CDS             8843..8995
                     /locus_tag="R8G18_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HEE9797392.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8995..9372
                     /locus_tag="R8G18_003802"
     CDS             8995..9372
                     /locus_tag="R8G18_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797393.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2442 a         2490 c         2017 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaata atgatgctat tgtttcaggc gtcaacccac cccgccccac agaatgcagg
     8701 gcaatacttc gagttaccag acagtggtca ggggttaagt agccagcccg aggcgtaaga
     8761 acatgacggc agggttcaat ctttaactat gcagcaggtt ttttgttccg ccaccccggc
     8821 gttaagggga aatgaggtca gcatggatac tatcgatctt ggcaacaacg aatctctggt
     8881 gtgcggcgtg ttccctaacc aggatggaac gtttaccgcc atgacgtata ccaaaagcaa
     8941 aacgtttaaa accgaatctg gcgcgcgttg ctggttagcc agaaacactg actgatgagg
     9001 ctgacgatgg aatttaaaga tttaccgtct gatgtacaga aaacagcagc tcatacattg
     9061 cattctgtgc tgcgagaaat cgggaaagat attgcaagcg agccagcaaa agatctggcc
     9121 cggaaaatca agaccgcttt cgttgagctt tataatgttg gcactgactc tgaaactgtc
     9181 gagaccaaga ccgtaagttc accaatattc tcacttgagc caagcgtatt atcaggtgaa
     9241 atatgcaccg agatttccag cgaactgctt ccggtaatac gagaagcaat tcgccgtcgc
     9301 ggtttggatg gaagttatga tcatgacgtt ctgcaagttc tcaggacaat ggtgacttca
     9361 ctggggattt gatccctgcg tcctctaagc ttttgatata gcggtcctcc aagaagcggt
     9421 aaattttgtc gaaatcatct tttccctctg gtttattcag aggattacag gacccgtaag
     9481 tagcggcata catttcaac
//
LOCUS       DAQSWQ010000146         9496 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.148, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000146 DAQSWQ010000000
VERSION     DAQSWQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.148"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            122..952
                     /locus_tag="R8G18_003803"
     CDS             122..952
                     /locus_tag="R8G18_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HEE9797394.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="R8G18_003804"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="R8G18_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HEE9797395.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="R8G18_003805"
     CDS             complement(2081..3541)
                     /locus_tag="R8G18_003805"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HEE9797396.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTVVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="R8G18_003806"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="R8G18_003806"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEE9797397.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="R8G18_003807"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="R8G18_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HEE9797398.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="R8G18_003808"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="R8G18_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HEE9797399.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="R8G18_003809"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="R8G18_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HEE9797400.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="R8G18_003810"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="R8G18_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HEE9797401.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="R8G18_003811"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="R8G18_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HEE9797402.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2259 a         2485 c         2376 g         2376 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc acggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAQSWQ010000147         9402 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.149, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000147 DAQSWQ010000000
VERSION     DAQSWQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.149"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="R8G18_003812"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="R8G18_003812"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEE9797403.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="R8G18_003813"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="R8G18_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HEE9797404.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="R8G18_003814"
     CDS             1394..1915
                     /locus_tag="R8G18_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEE9797405.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="R8G18_003815"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="R8G18_003815"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEE9797406.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="R8G18_003816"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="R8G18_003816"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HEE9797407.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="R8G18_003817"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="R8G18_003817"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEE9797408.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="R8G18_003818"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="R8G18_003818"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEE9797409.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="R8G18_003819"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="R8G18_003819"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEE9797410.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="R8G18_003820"
     CDS             complement(6557..7450)
                     /locus_tag="R8G18_003820"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEE9797411.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="R8G18_003821"
     CDS             complement(7447..8100)
                     /locus_tag="R8G18_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEE9797412.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="R8G18_003822"
     CDS             complement(8111..9370)
                     /locus_tag="R8G18_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEE9797413.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2092 a         2667 c         2493 g         2150 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gccggaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaaaccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa ac
//
LOCUS       DAQSWQ010000148         9244 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.150, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000148 DAQSWQ010000000
VERSION     DAQSWQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.150"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..96
                     /locus_tag="R8G18_003823"
     CDS             <1..96
                     /locus_tag="R8G18_003823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEE9797414.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="R8G18_003824"
     CDS             complement(152..487)
                     /locus_tag="R8G18_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HEE9797415.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="R8G18_003825"
     CDS             complement(491..835)
                     /locus_tag="R8G18_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEE9797416.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="R8G18_003826"
     CDS             complement(837..1010)
                     /locus_tag="R8G18_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797417.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="R8G18_003827"
     CDS             1111..1296
                     /locus_tag="R8G18_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797418.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="R8G18_003828"
     CDS             1257..1376
                     /locus_tag="R8G18_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEE9797419.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="R8G18_003829"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="R8G18_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEE9797420.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="R8G18_003830"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="R8G18_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEE9797421.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="R8G18_003831"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="R8G18_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEE9797422.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="R8G18_003832"
     CDS             4176..4544
                     /locus_tag="R8G18_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HEE9797423.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="R8G18_003833"
     CDS             complement(4652..5053)
                     /locus_tag="R8G18_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEE9797424.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="R8G18_003834"
     CDS             5295..5588
                     /locus_tag="R8G18_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HEE9797425.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="R8G18_003835"
     CDS             5660..6319
                     /locus_tag="R8G18_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEE9797426.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="R8G18_003836"
     CDS             6411..6857
                     /locus_tag="R8G18_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEE9797427.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="R8G18_003837"
     CDS             complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="R8G18_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HEE9797428.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
     gene            8348..8767
                     /locus_tag="R8G18_003838"
     CDS             8348..8767
                     /locus_tag="R8G18_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEE9797429.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            8767..>9244
                     /gene="umuC"
                     /locus_tag="R8G18_003839"
     CDS             8767..>9244
                     /gene="umuC"
                     /locus_tag="R8G18_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HEE9797430.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT         2378 a         2050 c         2146 g         2670 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca gtcataatca
     7981 ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta ttaataaatt
     8041 ttacttcatc taattcatag ttagccgggc gggatgcgtc aatgtcttta tttctattaa
     8101 tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat tctatttttt
     8161 tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg ccacgtgagc
     8221 acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga tagcctgaat
     8281 cagtattgat ctgctggcaa gaacagatta ctgtatataa aaacagtata acttcaggca
     8341 gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt ttccgctatt
     8401 tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgtcg aacagcgcat
     8461 cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca aagcaagtgg
     8521 tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg atagcgctat
     8581 taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta cggtgaaaaa
     8641 attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact cgcccattac
     8701 catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg ttaaggcgat
     8761 gcgctgatgt ttgctctctg tgatgtaaac gcgttttatg ccagctgtga gacggtgttt
     8821 cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg ttgcgttatc
     8881 gcccgaaacg ctgaggcaaa ggcgctgggt gttaaaatgg gcgatccctg gttcaaacaa
     8941 aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga gctttacgca
     9001 gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt cgagatttac
     9061 agtattgatg aggcattctg cgatctgaca ggtgtgcgta attgtcgcga tctgactgat
     9121 tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt tggtatgggg
     9181 atcgcccaga ccaaaacgct ggctaagctt gccaatcatg cggcaaaaaa atggcagcgg
     9241 caga
//
LOCUS       DAQSWQ010000149         9212 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.151, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000149 DAQSWQ010000000
VERSION     DAQSWQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.151"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            73..732
                     /locus_tag="R8G18_003840"
     CDS             73..732
                     /locus_tag="R8G18_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HEE9797431.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="R8G18_003841"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="R8G18_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HEE9797432.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="R8G18_003842"
     CDS             complement(1860..2570)
                     /locus_tag="R8G18_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HEE9797433.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="R8G18_003843"
     CDS             3004..3294
                     /locus_tag="R8G18_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HEE9797434.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="R8G18_003844"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="R8G18_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HEE9797435.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="R8G18_003845"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="R8G18_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HEE9797436.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="R8G18_003846"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="R8G18_003846"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEE9797437.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="R8G18_003847"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="R8G18_003847"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEE9797438.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="R8G18_003848"
     CDS             complement(7538..7837)
                     /locus_tag="R8G18_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HEE9797439.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="R8G18_003849"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="R8G18_003849"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HEE9797440.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9212)
                     /locus_tag="R8G18_003850"
     CDS             complement(9049..>9212)
                     /locus_tag="R8G18_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797441.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2327 c         2268 g         2331 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atctacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc ta
//
LOCUS       DAQSWQ010000150         9073 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.152, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000150 DAQSWQ010000000
VERSION     DAQSWQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.152"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(507..1274)
                     /gene="fecE"
                     /locus_tag="R8G18_003851"
     CDS             complement(507..1274)
                     /gene="fecE"
                     /locus_tag="R8G18_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HEE9797442.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="R8G18_003852"
     CDS             complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="R8G18_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HEE9797443.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="R8G18_003853"
     CDS             complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="R8G18_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HEE9797444.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="R8G18_003854"
     CDS             complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="R8G18_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HEE9797445.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="R8G18_003855"
     CDS             complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="R8G18_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HEE9797446.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="R8G18_003856"
     CDS             complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="R8G18_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HEE9797447.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="R8G18_003857"
     CDS             complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="R8G18_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HEE9797448.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8344..9041
                     /locus_tag="R8G18_003858"
                     /pseudo
     CDS             8344..9041
                     /locus_tag="R8G18_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1987 a         2510 c         2586 g         1990 t
ORIGIN      
        1 acacttttta gaaaaatcga tggtcagaaa ctggattagc aattccgttc cagggttgct
       61 tttgatttac gttggcgtct gatcattgat ttatcctcaa aagcccaacc tcattggtaa
      121 tgaaccagct ccgtgaatgt ccgctctggc acagagcgaa attttttgat ctcccccccc
      181 tgaaatctaa acttagtcat gtcacgtttt tgggtttcta aaattttaac ttcgcgtttt
      241 tcgttgccgt aagggttata cagaaatgtc cgttaagcag agttcaaaat tgattgccgt
      301 gatcacgact ggtttgaaag ccgcgcccaa gcctgtacag ctctggtttg cgttgattat
      361 gaacctgtca gcctaaagca agcggatgga cgatgagtat tggtaatctt tcagagtccg
      421 gaaaagttca gccccagtct gaacaggctt gctggcgcca gtccagtttc attcagtcgt
      481 ggtttggttc ttacggcctg tgcaatctac ctcattaggc acatcggcct gccagatacc
      541 ggctcggggt gtatttccgc ttccacgctg aatactgttc tcagcaatcc tggggtcatc
      601 acctcttctg gtgtgccttg cgccataaca tgtccgtttg ccattaccac cagttgatcg
      661 cagtaccggc tagcctgatt aaggtcgtgc agcacagcga ccaccgtttt cccctgagtc
      721 cggagttcgc ccatcaaccg catcaggtcc acctggtgat tgatatcaag ataggtggtt
      781 ggctcatcaa gtaatacaac gggcgtattc tgggccagga ccattgccag aaatgcgcgc
      841 tggcgctgac cgccggaaag ctcggttaac cgacgaacgg caagatgatt gatccgggtc
      901 tggttcatgg cgacattaac tcgtgcattg tcttcagcgg agagacgccc ccagagtgac
      961 agccagggat tacgaccata cgaaaccagc tcctggactg tgatcccctc tggcgttaaa
     1021 tggtgctgag gcagcagcga aagcctgcgg gccaactggc gcgatgagag catatttatg
     1081 ggattatcgc cgagaaatac ggtgccagac tgcggcatta aaagccgcga aaaacagttt
     1141 aacagcgtcg atttcccgca accgttagga ccgatcaggg cggtgatctt ccccgttggc
     1201 agtgagagtg aaacgtcgtt aagtaccttg tctgtcccgt aattgaccgt cagattttca
     1261 gttcgtaaag tcatttatcg cattctcaca agcaaccaga caaaccacgg cgcaccgata
     1321 atggcggtca gcacgccaac cgggagctcc agtgggggat gaatgattct cgccagcaga
     1381 tcggcaacca ccaacagcaa cgcacctgtc agggccgaaa caggcagcag tctgcggtga
     1441 cgtccaccgg tgatgctacg catcatatgc ggcaccacga gaccaataaa gctaatcggg
     1501 ccgcaggcgg ccacgccggt agatgtcatg gcgacagcta gtaacaaagc ccagaatcgg
     1561 gtatagggca ccgacacacc gagcgtggtg gcgcgcgcat cgccgagtgc aaggaggtcg
     1621 agatcgcggc aaaaactcag gctcagcggc agaaataaaa tcatcagcgg gatggcaatc
     1681 ttcacaaagc tccagtcacg gccccataag ctgccggtca gccacagcag ggcgttgttc
     1741 acatcctgcg ggcgcgagag catcagataa tccgtcaggc tggcccagca tgcagaaagc
     1801 gccacgccgg tgagtgccag cttcatcggc tggtgggtct ttgccagcat cttcagcaat
     1861 atcaaccccg ccatgccgcc cgcaaaggcc agcagcggca gcaccatcac gggcagtgac
     1921 ggcataagaa gtagagcccc cacagaggcc aggctggcgg catggttaac gccgagaata
     1981 tccggtgatg ccagagggtt gcgcacaatc ccctgtatca gcacgcccgc cacggcgagg
     2041 gctgcaccga caaacagtgc cagcagcaag cgcggcagtc ggtactccat caatacataa
     2101 taatgctcgc gtccggcctg ccagtcggtc agcagcgcgc gccacggcac ggggatcact
     2161 cccatatgga gtgataacag cgcacagccc gccagggcaa gggtgatgaa aataaccagc
     2221 gcaattttca tcctcgcctc ctcacaagcc agacaaagca agggctgcca atcagcgcca
     2281 gcactgcgcc tgcgggcaga tctccgggga aggccagcgc gcgtgcgagt acatctgcca
     2341 gcagcatcag cgtggccccc agcagcatgc tcactggcag tacgttgcgc tgatcgaagc
     2401 ctgcccagaa gcgcgccaga tgtggcacca gcagaccgat aaacgccacc ggacctgcca
     2461 cgctgacgca cgcaccaacc agaagcagca ctaacatatt gatgaccaaa cgtagcctcg
     2521 tcaggttcac tcccagcgta tgggcggtgc tgtcgctgag gttgagcagg ttcagttgat
     2581 tcgccagcag caacacgaca gggactgcag tgaccaccac cggcaagagc tgccagacat
     2641 cctgccagcg ggcgtgggac actccgccag ccagccagta aaagatgccg taagcatgat
     2701 cttcggccag cagcagggtg atgcgggtaa ggcccataca aaaggccgac agcgcgatac
     2761 ccgcgaggat cagtttgttt ctgtcatggg tatgacgaaa tccgcctcct gcggtcatga
     2821 ccagcagcca gctcacgccg cccccgcatg ccgcgatgaa cgacagagaa tagcctgcaa
     2881 tcggcgtcgg actcagcgcg ctggtaagcg ccatagccag cgccgcgccg ctgttaatgc
     2941 cgagcagtga aggagaggcc attgggttgt gggtcagggt ttgcagcagc gtgcccgcga
     3001 gcgccaggct tgcgccgatc agaacggcga ccaggcttcg tggcaaacga aggttttgca
     3061 ccagcgcttc tggtagcgtt ggcgtgtgtc caggcagcag ggcgcgggtt gcatctgctc
     3121 cggaaacagg aatggccgag tagcaaaaca gactcagcca gaaaataata ataagtgctg
     3181 caacgggaag cccccacagc agcaccgggt gttttatcgc ggtcatttca caacggtaag
     3241 cggctgatgg tggaagattt ttaccgtgtc agcggcaata cgctctgcag caaaaatacc
     3301 gcgcatccgc gcccaggtgt tactgtcgac cgaagcaacc tgctgcttct gcgcggcggt
     3361 taacatctgc cagagcggat cttgttgcca gcgtttaaca atgctctctt cgcgatagtg
     3421 ggcaaccagc agccaggcag gattgaccgc cagcagttgc tccaggccga tggacggcat
     3481 ggacgcaccc gccatcgcag cgggaacgtt cagccccaga gaggccagca cgctgccggt
     3541 ccaggtctcc tgagtatgca ggttgaattg ctgttcgcgt gatgtgccaa aggccacgcg
     3601 tgtccctttg ggaagctggc tggcccactg cgccatcctc tctttatgtt gttccagacg
     3661 tgcctgcatc tctcgctttt tacccaccat ttcgccgatg atagccgcag attgcaaatt
     3721 ttcagcgtag gtttcgttgc gggacttaag cagcagtacc gacgcgattt gctgcaaggc
     3781 gatgtaaacc cccgcatggc gactgctgtc ggcaatgatc aggtctggtt tcagagcggc
     3841 aatggcttcc aggctcggct gcgcgcgcgt tccgacggac tgccacggtt tcaggtgcgc
     3901 acgcacttcg ggcaggatgc gttttgcatc gttatcgtcg gcaataccga tcgggctgac
     3961 gtccacggcg gccagcgcat cggcgaacga gagttccagc accacaatcc gttgtggcgt
     4021 tttttcgagt gtaaacgtgc cgtgttcgtc ctgaaccgtg gcggcaaagg cgtggctgat
     4081 caccagcagc aggcctgcaa aaagaaaacg gataaatgcc aacataatca cattccagct
     4141 aaaagcccgg caagccgggc gttaacacat cagaacttca acgccccctg catatacagc
     4201 gtgcgcggct ggcctgcata gatgcctttg ttgttgtcgt cataagagcg gatgaagtag
     4261 tcctggtcga agatgttttt cacaccgaat gccaggttca gatctgccat ctgcgggcca
     4321 aagtcataca ccacgcgtgc gccccagagc atgaagccgg gaatgcggcc ggtactgccg
     4381 tcggcgctct ctttcaccgt attggcgtta tccgcaaact ggctggactg gaaatcgctg
     4441 ttcagattga acatccagtt tcctggcttg tagtccacgc ccagcgtgcc tttatgtttc
     4501 ggggagaatg gtaccagatt gccgtaggtg tcgcctttct cgcggatttc cgcgttcaca
     4561 tacgcatagc tggcgtagat ggaaacgtta tcaagcgttg gcgttagcgt acccagatcg
     4621 taacgtgcct gcgtttccag cccggtatgg cgcgttttgc cacgtgcagt gacggtgtcg
     4681 ttggtctggt tggagtcgta ctgattgtta aagttaatca ggaacagccc catttccgcc
     4741 gtcagcgcgc cgtcgtcgta gcgggtaccg agttcccagg ttcgcgcttt ttccggttcc
     4801 acattgccgc tttgcacagc cttgccaatc tggctgtact gtacggtgcc gaacgagcct
     4861 tcagtgtttg cataaagatt ccagctgtca gtcaggtgat agagcacgtt caacgccgga
     4921 agcggtgcgt tatagctcac ttcttcgtgc gtgcctgtga tggcgttgtt ctggtatgac
     4981 tcgatatgtt cgaaacgcat acccggcgtg atggtccagt tgccgatgtc gattttgtca
     5041 tccagatacc aggcgtgcgc ctcggtgccg gaacgcgtat cgcggtcgta agggcttgag
     5101 ccggacggca actgcccgct gctggtggcg gtgtagtaac gcatttcatg cgttgattca
     5161 ttcacatagc gatagcccac gcccacttcg tgcgcggaag ggccgatcat aaagatctgg
     5221 ctgtagcgtg gctcaatacc gcgcacccag tagttacgcg gcgagagggt gatgcgtttg
     5281 ccttgctcca ggtagccgct gcgcagggtt tgggtgtaga acccctgaat gttgaattta
     5341 tgctggctgt ctggctggaa ctggtagccc aggctcgcca gcttgcgacg accccagaag
     5401 cggtcatacg ggcgggtgga ttgccagcga tcggcgtcgt aatccgcgcg agacaggcca
     5461 ccgggcatgt cggcttcacc gtcgtaatat tgcagcaggc tgttgaaggt gtgcacctca
     5521 tccggcgcat atttgctttt cagcatcagg tcgtcgatgc gggtggcgct gtgctcgcgc
     5581 cagtcactgc cgcgcgtgcc ggagtagagc agcgcggtgc caaaaccgtt gtccgctgtg
     5641 ccgcccacca tcaggttgtg cgtctctttc gggttgtttt gtgaagaggt tgggctgagc
     5701 tgaccttcca cgcccgcctc gataccaaag tcctgcggaa tggcacgggt aacaaagttc
     5761 accacgccgc ccacgctctg cggtccgtaa cgcaccgcac caccaccgcg taccacgtca
     5821 atggcatcca tgttgccgag cgaaacggga gccagtgaaa gctgcggctg accgtaaggg
     5881 gcgaagggga cggggatgcc gtccatcagg acggtcgagc ggctggcgag gcgcgggttc
     5941 aggccccgga tgccaaagtt catcgccagg tcgtggctgc cggtgccgtt gttttccggc
     6001 gcgctgacgc cagggatgcg gttaagtacc tcacgcatgg tggttgcgcc ggttttggcg
     6061 aaatcctcac ggcggatcac gtcacgcgcg ccagcatgtt caaatacgtc gttttcacgc
     6121 gcatcaccca gccagtcgcc gaccacggtc agggcatctt cttttggtgc gggcgcgggc
     6181 tccagcgtcc agctgttatt tcccagcggt tttacctgca gtccgctgcc gtccagcagt
     6241 tgttgcaggc cgctctcgac gtcgtaatcg ccgtgcaggc cgttgctctg cttgccgcgc
     6301 gtcaggctgg cgtcaaccga gagggtaaat ccgctgtgtg cggcatactg attgagcgct
     6361 ttatcgagcg atcccggtgc gatattaacc tgtgcagcaa aagcggaaaa cgagagaccg
     6421 gccagcggca gcaggctcag gcgaatggcg ttaaccaaag gtgttgtttt acgaaaaacg
     6481 cgtaacggcg tcataccttc cccatcatca tttttgttgt gttcagctat gagtcgaacg
     6541 agaagggtaa aaaggacaat cgaaataaga attattttcc ttacagtggt gaaatgttta
     6601 tccagtaccg cgtaatagac tgaattttaa cgggaagcgt ttgcgcgata acgttcagga
     6661 tcgcatcggt atttttcagc gggaacgtcc cgctcaggcg cagcccggca acggcgggat
     6721 cgcagcgcag cacgccgttg cggtaacggg ttagcgtggc tatcacctca cccagcggtt
     6781 tatcgctgaa gctcaggatg tccttcgtcc agcttgtact ctcgtcatcc agcggtttca
     6841 ctgcgccaaa ctcagaggcg ctgaactgca ggctttcacc agcgttcacg atgcgttttt
     6901 gcgcgggggc actggcgaga agcacttcca cggcgtgctg ctgcacgtca agctgcgtga
     6961 aattatcctg ctggcggacg gtaaattctg tccctaaagc agtgagctgg ccctgacggg
     7021 tcaggacacg gaaggggcgt tgcagggcat ctttcgcggt agtaatggcg atttcaccgt
     7081 accagagccg gacggtgcgc tgatgcgcat caaaacgcat atccgccgcg ctttgggtat
     7141 tcagcgtgag cagggagcca tcttccagtt gctgacggct gactgtgcct ttggcggtgc
     7201 ggtaatctgc ccgcagacct tcgccggttt ccgactgcca gagctgccag cctccgccag
     7261 cgccgagcaa cagcagtaat cctttcatca cgtgacggcg ggtgaggcgg gtatcgtgca
     7321 acgcccggct ggcaacgtcg ccaggcacac cgccaagctg gttgcgcagg ttttcaacct
     7381 gctgccaggc ccactggtta tcctgatcct gttcatacca ctgttgccag cgcgcttcct
     7441 gttgtgggct gacgcgctcg ccgcttagca cggcatacca gtgggaagct gaacgcagcg
     7501 cctgacggcg ggaatcggtt aacaaaggat tcataaccca tactccagac ggaacagcag
     7561 gcagtgctcg acggctttcg ccacgtattt tttcacggag ctgatggaaa caccgagttt
     7621 gtgcgcaatc tcgctgtatg tcagaccatc cagttgcgaa agcagaaacg cttcacgtgt
     7681 tttgccgttt agcccgtcca gcatgctgtc gaggagttgt agggtctcga gttggctttc
     7741 gcgttcctca ggtgaaggcg ctcccccctc cggcataagc gccagcatct ccagatacgc
     7801 tttttccagc gcgtttcggc gaaacaggtc caccatcacg cgtttggcga tagtgcagag
     7861 gaaggagcga ggatcgcgga tcgtcgagag cgtttcgctg accattaccc gcaaaaaagt
     7921 gtcctgggca atgtcatctg catcaaaagc agactggagt ttgcgcgtca gccagctttt
     7981 caaccagccg tgatgtgtgc cataaagcga ctcgaatgtt aaggaagctg tggtagtggc
     8041 gcggtcagac atgcggagtg catcaaaagt taattatcac gtagccatat taatatgaga
     8101 atagttatca ttacaattgg aaataaaatt gtttccaata gacattttta acatgttgtt
     8161 tttctaagtg ttataaggta ggtataaaat gggatggagc ctctgcttct ggcatgtgtc
     8221 ggtcagaatg actcatgatg tggtctgcta ttattgacat cctcactgcc ctaaaggata
     8281 gggatttcgg taatgctgtc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     8341 tgcgtggctt ccatttccat cagatgtcct tcctgctccg ctactgaagg cgtggtgcgt
     8401 aacggcaaaa gcactgccgg acatcagcgc tatctctgct ctcattgccg taaaacatgg
     8461 caactacagt tcacttacac cgcctctcag cccggtaagc accagaaaat cattgatatg
     8521 gccatgaatg gcgtcggatg tcgcgccagt gcacgcatta tgggcgttgg cctcaacacg
     8581 gttttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata caaccgggca
     8641 gtgatgtgat tgtctgcgct gaaatggacg aacagtgggg ctacgtcggt gctaaatcac
     8701 gtcagcgctg gctgttttac gcgtatgaca ggatacggag gacggttgtg gcgcacgtct
     8761 tcggtgaacg cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg
     8821 tcgtggtata gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc
     8881 acgttatcag caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac
     8941 atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca
     9001 aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc attacctcct
     9061 cccgaactca aaa
//
LOCUS       DAQSWQ010000151         8974 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.153, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000151 DAQSWQ010000000
VERSION     DAQSWQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.153"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..839
                     /locus_tag="R8G18_003859"
                     /pseudo
     CDS             1..839
                     /locus_tag="R8G18_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1453..1829)
                     /gene="rnpB"
                     /locus_tag="R8G18_003860"
     ncRNA           complement(1453..1829)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="R8G18_003860"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1862..3007)
                     /gene="garK"
                     /locus_tag="R8G18_003861"
     CDS             complement(1862..3007)
                     /gene="garK"
                     /locus_tag="R8G18_003861"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HEE9797449.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3104..3994)
                     /gene="garR"
                     /locus_tag="R8G18_003862"
     CDS             complement(3104..3994)
                     /gene="garR"
                     /locus_tag="R8G18_003862"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEE9797450.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4024..4794)
                     /gene="garL"
                     /locus_tag="R8G18_003863"
     CDS             complement(4024..4794)
                     /gene="garL"
                     /locus_tag="R8G18_003863"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HEE9797451.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4810..6144)
                     /gene="garP"
                     /locus_tag="R8G18_003864"
     CDS             complement(4810..6144)
                     /gene="garP"
                     /locus_tag="R8G18_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HEE9797452.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6519..8090
                     /gene="garD"
                     /locus_tag="R8G18_003865"
     CDS             6519..8090
                     /gene="garD"
                     /locus_tag="R8G18_003865"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HEE9797453.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            8239..8644
                     /locus_tag="R8G18_003866"
                     /pseudo
     CDS             8239..8644
                     /locus_tag="R8G18_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
     gene            8644..>8974
                     /gene="yhaV"
                     /locus_tag="R8G18_003867"
     CDS             8644..>8974
                     /gene="yhaV"
                     /locus_tag="R8G18_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HEE9797454.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT         2404 a         2292 c         2084 g         2194 t
ORIGIN      
        1 aatatctaca ggcaatatca atgttaattg cccattaaaa aatatttaca atgaaatcag
       61 gaggttgaac gtaattttta gttgtgggac tggagatata gttgatctat cctctctgga
      121 cttacgtaat gtcgatttag attattacga ttttacagat aaacatatgg ctaatactat
      181 tttaaatcct tttaaattgg attcaacaaa ttttactaat gccaacatgt tccaggttaa
      241 ttttgttagt tcaacacaaa acgccacaat ctcctgggat tatttactaa aaataacgcc
      301 tgttttaata agcattagcg atatgtattc tgaagaaaaa atcaagtttg tcgaaagttg
      361 tttaaatgag cttggagaca ttaccgaaga acaattaaaa attatgagat ttgcaattat
      421 aaaatctata ccaagggcaa ctcttacaga taaattagaa aatgaattaa caaaagaaat
      481 atataaaagc tcatcgaaaa tcaacaattg cttgaacaga attaaattac cagagatgaa
      541 agaattctca tcagaaaaaa atatatgatt acatcgatat aatcattgaa gattatgaaa
      601 atatcaaaga aaatgcttat ctggtcgatc caaaaattaa ttacactata gatttaaaca
      661 tagaagactc tagctcagag gaattccttt ttgataatag cctcgagaaa gacgaaaatt
      721 cttcggacaa ttgctttgag gtcgaaaaat ataacgcata tgaagcatat aactcagaga
      781 atctatattt taccagagag gaatatacgt atgattacga ccttttaaat gcaatataga
      841 cataataact tcttacaaaa tctataatta ataccgtaga tattcattca tcttaccctg
      901 tgcaccaaaa gcaaaaactc actgacgcgg gtttgaaact gatggcaacc gcaaaaatgc
      961 ctgatgcgct acgcttatca ggcctacgcc atctctacaa tatattgaat ttgcgtgctt
     1021 ttgtaggcag gataaggcgt tcacgcagca tccggcatga ataaagcgca ctcaacaatc
     1081 tgaaacccgc cggaacggtt tattacgtac atcaggtgaa actgaccgat aagccgcttt
     1141 cttttgggta tagtgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc
     1201 cgccgaagcg ggtttttacg taaatcaggt gaaactgacc gataagccgc tttcttttgg
     1261 gtatagcgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
     1321 gcggtttttt acgtaaacca ggtgaaactg accgataagc cgctttcttt tgggtatagc
     1381 gtcgtggaca gtcattcatc tttccgctcc tccaaaagca aaaacccgcc gaagcgggtt
     1441 tttacgtaaa tcaggtgaaa ctgaccgata agccgggttc tgtcgtggac agtcattcat
     1501 ctaggccagc aatcgctcac tggctcaagc agcctacccg ggttcagtac gggccgtacc
     1561 ttatgaaccc ctatttggcc ttgctccggg tggagtttac cgtgccacgg actgttacca
     1621 gccgcgcggt gcgctcttac cgcacccttt cacccttacc tgatcccgct tgcgcgggcc
     1681 atcggcggtt tgctctctgt tgcactggtc gtgggtttcc cccccaggcg ttacctggca
     1741 ccctgcccta tggagcccgg actttcctcc cctccgcccg tctcccccga agaggacgac
     1801 gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta tagagggttt gcgcgccctt
     1861 gtcaccccgc gttgcgcatt ccaatcgcca gtgtcgcggc gatattacgt gaagcacggc
     1921 agatattgtc ataagccccg cggaatgctt cgtccaacgt acctatgctg gtcaatacgc
     1981 tgaagaccgc atcgatgcca tgctgatgta caacgccaac atcatcggtc aggctacccg
     2041 caatgccaat caccggttta tggtacttct tcgccacgtt tgcaacgcca atcggtacct
     2101 tgccgtgaat actctggctg tcaatacgcc cttcaccggt gatcaccagc gtacaatcgt
     2161 gaatatgttc ttccagattc agcgccgtag tgacgatttc aataccactt ttcagttccg
     2221 caccaagaaa cgccatcagc gccgcgccca taccgcccgc agctcctgcg ccggggacat
     2281 ctttcacatc aacatgcagc gcttttttaa tgacatcggc atagtgagag aggttattgt
     2341 ccagctcaac aatcatcgct tcactggctc ccttttgtgg gccaaagatg cgcgatgcgc
     2401 cgttatcgcc caccagcgga ttggtgacat cacaagcgac gcgaatgacg caatctttta
     2461 agcgcggatc gaggccggaa atatcaatat cattcagagt attaagacta ccgccgccaa
     2521 aaccaatttc attgccgttg gcgtcgcata atttcgcccc cagcgcctgt accatgcctg
     2581 cgccgccatc atttgtagcg ctgccgccaa tgccgataat aatgtttgtt gcactgctct
     2641 ccagcgcctg caggattaac tcgcctgtgc cgcgtgaagt ggttacgagt ggatcgcgtt
     2701 tttccgcagg taccagctcc agcccactgg ccgccgccat ttcaataaac gcggttttgc
     2761 catcgccgga gatcccccaa ctggcattca ctttctcgcc cagcggccct gtaacccagg
     2821 cgtgacgttc agccccctgg gtggctgcaa tcatcgcttc caccgttcct tcaccaccgt
     2881 cggcaaccgg aacagaaaca tactgtgcat caggaaaaat ttcccgaaat cctttttcta
     2941 tcgcctgcgc aacctcgctg gcagataaac tttctttata agagtctggg gcgattacga
     3001 ttttcatacc tatgcctgtt accacatgac gccggagggc gtttctctta ttcggcctag
     3061 attccaggcc cggattgcaa tacaccatcc gggcacgacg tcattaacga gtaacttcga
     3121 ctttcgccag tttttcgtag tagcacgcca gggcgctatg atccgccgtt cctaaaccat
     3181 ctgcacgcag tgcctgcatc atctccataa ccgcagctgt gagcagcagt tgtgcgccga
     3241 cgccgtgaga agtatccagc gcattcgcca gatccttaat atgcagatca atgcggaagc
     3301 ccggcttgaa gttgcggtcc atcaccatcg gcgctttggc atccagcacg gtactgcccg
     3361 ccagcccacc gcgaattgcc tgataaacca ggtccgggtt aacgcccgct ttggttgcca
     3421 gcgttaacgc ttctgacatc gcagcaatat tcagcgccac aatgacctga tttgccagtt
     3481 tggtgacgtt acctgcaccg atttccccgg tatgcaccac ggaacccgcc atcgctttca
     3541 tcaaatcata gtatttgtcg aaaatagcct tgtcgccgcc caccatcact gacagcgtac
     3601 cgtcgatggc tttcggttca ccgccgctca ccggagcatc cagcatatca atgcctttcg
     3661 ctttcagcgc ttcgctgatt tcacggcttg ccagcggtgc gatagaactc atatcgatca
     3721 ataccgtacc tggcttcgcg ccttcaataa tgccattctc acccagcgcc acctctttca
     3781 catgagggga gtttggcagc atggttatga tgacgtcgca ctgttcagcg atcgctttag
     3841 ccgtagacgc tgtttctgca cctgcagcaa tcacgtcagc aatagcttct gggttacggt
     3901 cagcaaccac cagcgagtaa cctgctttca gaaggttttt actcattggt ttacccataa
     3961 tccccaggcc aataaaacca actttcatag tcatatcaac catctctctt gttgcggtgg
     4021 tggttatttt ttaaaggtat cagccagttt ctgagtggca gagcggaaga cgccaagatc
     4081 gctgccgaca gccacaaacg tcgcgcccca ttccagataa cgacgcgcat cggcttcgac
     4141 cggcgcgaga ataccgctgg gtttgccgtg cgcgctggca cggttaaaaa tatgctgaat
     4201 tgctttttgt acatccggat gcgaggcatt gccgagatgg cccaatgccg cagccagatc
     4261 gctggggccg acgaagatac cgtctacacc ttcggtagcg gcaatagcat cgacgttatc
     4321 tacgccctgc tgactttcta tctggaccag aatagtaatg ttcttgttcg actgagcgaa
     4381 ataatccgcc acggtgccaa acatattggc gcggtgagaa acggagacgc cgcgaatgcc
     4441 ttccggcggg taacgagttg atgccaccgc ctgctctgct tcctcttttg tttctacaaa
     4501 aggaatcagg aagttataga aaccgatatc cagaagacgc ttaataatta ccggctcgtt
     4561 ggtcggcact cgcactactg gcgcgctggc gctgcctttc agggccatta actgcggaat
     4621 aaacgtggaa atatcgtttg gcgcatgttc gccatccagc accagccagt caaacccagc
     4681 caaaccaaga acttcagtgc taatcgggtt agagagtgct gaccagcaac caatttgtac
     4741 ctgtttcgca gccagtgcgg ctttgaattt attcgggaaa acatcgttat tcatcgctta
     4801 tacctttgct tatttctgca attccatacg tttaatgtcg ccaactacga agaggtagca
     4861 gaccatcgcc atcagcgctg aacatcccac gaaaaccagt gctgcattga aggagtgcag
     4921 ttcacttacc aggtagccaa tcaccagtgg agtgacaatg gaggcaacat tgccaaagac
     4981 gttaaagaca ccgccacaga ggccaacaat ctctttcggc gcggtgtcag aaatcaccgg
     5041 ccagcccaga gcaccaaatc ctttgccaaa aaaagccagc gccatcagca tgaccaccag
     5101 cgtggtgttg ttggtgtagt tacataagat gatggtggaa accagcaaca ttcccagcac
     5161 aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt ttgatcagat aatccgagaa
     5221 gacacctccc agcacgccgc ccgcaaaacc acacagtgcc ggaatcgagg cgaccagacc
     5281 cactttcaga atcgacatgc ctttttcctg caccagataa atcgggaacc aggtgaggaa
     5341 gaaccaggtg atggtgttga taaaatattg tccgaaaaat acgcccaaca tcatgcggtt
     5401 agagagcaat tgcttgatgt aatgcagttt gggaccgctt gctgccgcgc tgcccggctt
     5461 tttgtggtcc atatcgacca ccgcgccatt ttcagagata aacttcagct cttccgcaga
     5521 catacgtggg tgatctgtcg ggttatgaat caacttgatc cacagcgccg tcagcacaaa
     5581 accaatcccc cccataacgg tgaagacgtg ctcccagccc caagcgaaag tcagccagcc
     5641 aagcagcggc gaaaagagcg ccagcgagaa atattgcgcc gagttaaaga tggcggaggc
     5701 agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata cgggcgttcg ccgggaatga
     5761 tggcgcttcc gagaacccca gcataaagcg cataaagaac atggagatcc ctgcccaggc
     5821 cagcgggaac atatcaacaa agccttgcag gaaggtgaac agcgaccaga aaaagaggct
     5881 gtaggtgtaa acttttttcg agccaaactt atcaagcaac cagccgccgg ggatttgcat
     5941 cagcaagtag gcccagccaa aagcagagaa gatgtaaccc atcgaaaccg cacttaactg
     6001 caactctttt gccacttcgg taccagcaat agacagcgtt gcacgatcgg cgtagttaac
     6061 ggcggtaaca ataaaaataa tcagtaatat taaatagctg gtatgcacgc ctttcttttt
     6121 tacgtcaacg gtgtccagaa tcattttatt tacctcgggt acttatgctg atttttatta
     6181 ttatggggaa ggtgttattt atgagtttta tttatgccgt tacgacaatg aactcgggaa
     6241 ttagtataag cagcgggaga ataataatca ttgtgcaaat gctaatttaa tcagtactat
     6301 ttaaatatta ttttgagcat atgcacataa ggttgcgcgc taaagcacag atttgcgctt
     6361 taccttaccg ggcggcactg caatccctga aatgattgac attgatcaca tttctgcgtt
     6421 taaactcctg acattcttat ttcacccaat gaagtcattt atttttaaat gagaccagat
     6481 cctcatttta ataacccctg gctggagaat attgcacaat ggccaacatc gaaatcagac
     6541 aagaaacgcc aactgcgttt tatataaaag ttcacgacac agataatgtg gcaattattg
     6601 ttaatgataa tggcctgaaa gcaggaacgc gttttccgga tgggctggaa ttaattgaac
     6661 atattcccca ggggcataaa gtcgcattgc tggacattcc agctaatggt gaaattattc
     6721 gttatggcga agtgattggt tacgccgtgc gcgcaatccc acgcggaagc tggatcgacg
     6781 aatcaatggt tgtactaccg gaagcgccgc cgttacacac gctgccactg gcaaccaaag
     6841 tcccggcccc cttaccgccg ctggaaggat acacctttga gggctatcgc aatgccgatg
     6901 gcagcgtggg caccaaaaac ctgctcggca tcaccaccag cgtccactgt gtggcaggcg
     6961 tggtggacta tgtagtaaaa atcattgaac gcgatctgct accgaaatac ccgaacgtcg
     7021 atggcgtggt ggggctgaat catttgtacg gctgtggcgt ggcgattaac gcaccggcgg
     7081 cagttgtgcc tattcgtacc atccacaata tttcgctgaa ccctaacttt ggcggcgaag
     7141 taatggtgat tggtctgggt tgtgaaaagt tgcagcctga gcgcctgctg gtcggcacgg
     7201 atgatgtgca agctattcca gtagaaagcg ccagcattgt cagtttgcag gatgaaaagc
     7261 atgtcggctt tcagtccatg gtcgaggata ttttgcaggt cgccgaacgc catctacaaa
     7321 aactgaatca acggcagcga gaaacctgcc cggcttcaga actggtcgtt ggtgtgcagt
     7381 gcggtggcag cgatgcgttt tctggtgtaa cggcaaaccc ggcggttggc tatgcgtctg
     7441 atctactggt gcgctgcggc gcaacggtga tgttttcaga agtaacggaa gtgcgtgacg
     7501 cgatccatct gctgacacca cgcgcagtga acgaagaggt cggcaaacgg ctgctggagg
     7561 agatggagtg gtacgataac tatctcaata tgggaaaaac cgaccgcagc gccaaccctt
     7621 cgccgggcaa caagaaaggc ggtctggcaa atgtggtgga gaaggcactc ggctccattg
     7681 ctaaatcggg taaaagtgca attgttgaag tgctgtcgcc cggtcaacgc ccgactaaac
     7741 gcggattaat ttacgccgcg acgccagcca gcgattttgt ctgtggcacg caacaggtgg
     7801 cttcgggtat caccgtacaa gtgtttacga ccggccgtgg tacgccgtac ggcctgatgg
     7861 cggtacccgt aattaaaatg gcaacccgca ccgagctggc gaaccgctgg tttgatttaa
     7921 tggatattaa cgcgggcact atcgctaccg gcgaagagag cattgaagag gtgggctgga
     7981 agttgttcca ctttattctc gacgtcgcca gcgggaagaa gaaaaccttc tcggatcaat
     8041 gggggctgca taaccagctg gcggtgttta acccggcacc ggtgacctga tttcttttgg
     8101 aacgtccctc gcaaaacatg gccttagtgc catgttttta ttgtttaaag cccccacgtc
     8161 cattaataat gcatttgcat taccttagtt caagcttata atttgagcag aaaacaggct
     8221 gtaaaaggac aatgaatcat gcccgctaat gctcgctctc acgctgtact gaccactgaa
     8281 tcaaaggtca cgatacgcgg acaaacaact atccccgcgc cagtgcgtga ggccttaaaa
     8341 ctgaagccag gccaggacag cattcattac gaaattctgc ctggtgggca agtatttatg
     8401 tgccgactgg gagatgaaca ggaggatcat actatgaatg catttttgcg ttttctggat
     8461 acagatatcc agaacaaccc gcaaaaaact cgtccattca acattcaaca ttcaacattc
     8521 aacattcaac attcaacatt caacattcaa cattcaacat tcaacattca acattcaaca
     8581 aggaaagaaa cttgtcgctg gcatggacgt caacattgat gatgagattg gcgacgacga
     8641 ataatggatt ttccacaaag ggttaatggt tgggcgctat atgctcatcc ctgttttcag
     8701 gaaacctacg acgctttagt tgccgaagtc gagacattaa agggaaaaga tcctgaaaat
     8761 tatcagagaa aagccgccac aaagttattg gcggtagtcc ataaagtgat tgaggagcat
     8821 atcacggtca atccatcatc accggcattc cgtcatggca agtcgttagg ctctgggaaa
     8881 aataaagact ggtcacgggt aaaatttggt gcaggtcgtt atcgtctctt ctttcgttat
     8941 agtgaaaaag agaaagtcat cattctggga tgga
//
LOCUS       DAQSWQ010000152         8840 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.154, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000152 DAQSWQ010000000
VERSION     DAQSWQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.154"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="R8G18_003868"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="R8G18_003868"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HEE9797455.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="R8G18_003869"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="R8G18_003869"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HEE9797456.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="R8G18_003870"
     CDS             complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="R8G18_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HEE9797457.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="R8G18_003871"
     CDS             complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="R8G18_003871"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HEE9797458.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2167 a         2050 c         2261 g         2362 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggagcgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacttt gatgtaatct gttgaaataa tgggaatcgt aggacgcata
     4921 aggcgtttac gccgcattcg gcaatcggtg caaatgccag ataagacact aacgcgtctt
     4981 atctggccta caggtgatta gtctttatct gccggactta acgtcactga agagagataa
     5041 ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca tcgcagattt cttctggcta
     5101 ctgatggttt taatactgcg gttcagcttt ttagcgatct cggtcaccag gaagccttcc
     5161 gcaaacaggc gcagaacttc actctctttt ggcgagagac gcttgtcacc gtaaccacca
     5221 gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt ccctttctgc
     5281 agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac gatcccttcg
     5341 atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatagt cagaacaatg
     5401 atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc atcgccgtac
     5461 ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt cggcaggttg
     5521 ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc aatttgctca
     5581 agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat tacgttcata
     5641 ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt caatgtcgct
     5701 gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca gcgtttcaca
     5761 taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac gatgagccgt
     5821 ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct taacatcatc
     5881 cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg cattttcatc
     5941 tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa
     6001 gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac caggcccaat
     6061 ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa gattagacgg
     6121 attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg gtgtgataca
     6181 ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg ccgaagtaac
     6241 atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa cttccgggcc
     6301 agcggcgagc attttgatat gcacagagta gcgtgtacca agcccatccc gcgttttgat
     6361 gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc agccagaatg ccagcgggtc
     6421 cgcctttcca taacgatcgt tttgggtctg gttgataaac gggaagtgca aattatccat
     6481 ttcatgaata cttacgcctt ctccggtgtc cagaatgcgg aatgtcaggc ggtcttcaga
     6541 ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg tcacggcata
     6601 ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg
     6661 tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg caggcaacac
     6721 tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct cacttttcca
     6781 gctatcgtcc gcaagcatgt tcgctaactg tatttcatcg accagccgca ccagcacatc
     6841 tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc tctccgccag
     6901 ggactgtgca ggttctttca gcgcatcgcc aatgtttttc ataaaggtca tccggccctg
     6961 ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg
     7021 atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg
     7081 gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct gttccgccat
     7141 ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt tgttacttat
     7201 gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg agcggcagca gtgagactat
     7261 ctcttcattg attgcccgta aaatgcgcaa ttcgttattg accgccgtgt tgggtagact
     7321 ttctgtactg cgactggaga aatggcggaa tgtggtatag ccaaataacg ccagcgccag
     7381 caaaccgatg ttcagcagca gtggcagcag aatgttttgc aacgtatcca gcaataaggt
     7441 gccataagga acctgccaga ccaggcgcat atcggtagag ttgagtgccg aggagatttc
     7501 aatcttcgta ctgttaaagt ggatactgac gctatccgtt ccttctttct cattatcatt
     7561 gtctcccgtt gccgtcgtgt ctggctcaag gcggaaactg tccagcggca tacccggtgg
     7621 gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc ccggctggtt
     7681 gaacgtggta cgcaaggtaa agtaatgacc gttctgccag gccaggcggc gcatgttaga
     7741 aaaactttcg cgttcatcga gggcgttggc ctgttgcaac atttctgcac gacgtgaatc
     7801 aacaatgtca ctgacggtcg attctttaaa tccggaggtg agatctttga ggggcagggt
     7861 tgagatcagt accagactgt tatcctgacc attcaggtaa tacatcgacc acggtacatt
     7921 ttctgcaccc cacaatgtat ccagataagt ggacatccgc tgagtcatct caagcgttga
     7981 gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct ccagatagta
     8041 gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc
     8101 ggcaatgttg tcgtagatct gccaggtcac gtaacgccag gtatcgacgc gcttttggat
     8161 tgcgtgggta atgtcgacaa tctggtaact tttatctttc agccaggcgt taacggcgct
     8221 ttgtaccatt acccccatcg tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt
     8281 aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct gacgcattcg
     8341 tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt acagtgtgaa
     8401 attccagact cttacctgat tgatgattct ctattctccc gtgatattta tcatcctgca
     8461 ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctaca aaaagcccaa
     8521 cgaaattaat tgcaaataaa cgaagattgc tggaaattat gctgatgtta attatttgtg
     8581 aaatagttga caagagttat agtttttctg tggtagcaca gaataatgaa agtgtgtaaa
     8641 gaagggtaaa aaaaaccgga tgcgaggcat ccggttgaaa taggggtaaa cagacattca
     8701 gaaatgaatg acggtaataa ataaagttaa tgatgatagc gggagatatt ctagttgcga
     8761 gtgaaggttt tgttttgaca ttcagtgctg tcaaatactt aagaataagt tattgatttt
     8821 aaccttgaat tattattgct
//
LOCUS       DAQSWQ010000153         8773 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.155, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000153 DAQSWQ010000000
VERSION     DAQSWQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.155"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(406..612)
                     /locus_tag="R8G18_003872"
     CDS             complement(406..612)
                     /locus_tag="R8G18_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HEE9797459.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            925..1014
                     /gene="kdpF"
                     /locus_tag="R8G18_003873"
     CDS             925..1014
                     /gene="kdpF"
                     /locus_tag="R8G18_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEE9797460.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1014..2687
                     /gene="kdpA"
                     /locus_tag="R8G18_003874"
     CDS             1014..2687
                     /gene="kdpA"
                     /locus_tag="R8G18_003874"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEE9797461.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2710..4758
                     /gene="kdpB"
                     /locus_tag="R8G18_003875"
     CDS             2710..4758
                     /gene="kdpB"
                     /locus_tag="R8G18_003875"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEE9797462.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4767..5339
                     /gene="kdpC"
                     /locus_tag="R8G18_003876"
     CDS             4767..5339
                     /gene="kdpC"
                     /locus_tag="R8G18_003876"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEE9797463.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5332..8016
                     /gene="kdpD"
                     /locus_tag="R8G18_003877"
     CDS             5332..8016
                     /gene="kdpD"
                     /locus_tag="R8G18_003877"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HEE9797464.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8013..8690
                     /gene="kdpE"
                     /locus_tag="R8G18_003878"
     CDS             8013..8690
                     /gene="kdpE"
                     /locus_tag="R8G18_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HEE9797465.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1840 a         2286 c         2529 g         2118 t
ORIGIN      
        1 gcaccgagca ggggattgac cggatggccg gacgtcactc cgccggggca caccgaacag
       61 gccacaccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatttctca tccttgtttt
      181 atcgggttat ttgtccgctg acaggcaaga ccctacagat ttataagaaa gccttaagtg
      241 agatatccgt cagatagaaa caattaggaa aatatttata tatatttcat gaatatatgt
      301 ttcgattggt ataacggtgt ctaaatattc ctctgaacat cttcctgata caaaaaaccg
      361 ccagcgtttc cgctggcggt tttcaatgta ttcagaactc agcaatcagt aaaaatcacc
      421 ggttgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg accagacgcg
      481 gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag
      541 aacgcccgct gcaaccgcat atgtacggcg taaaaagata agccatgcag gatattctct
      601 gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca ccggaaaaat
      661 gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac
      721 cacttatttt tgcttattga tggtttattt acattcactc tgtaattaag ttacacaaaa
      781 gttaaattaa tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta
      841 caccccgccc gcagattttt gcgaaatctt tgcagccaga attctactct tccggtatca
      901 cttttaggcc actggaggtg cactgtgagt gcaggcgtga tagccggcgt gttgctggtg
      961 tttttattac tgggttattt ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg
     1021 cgcaagggtt cttactgatt gccacgtttt tactggtatt aatggtgctg gcgcgtcctt
     1081 taggcagcgg gctggcgcgg ctgattaatg acattcctct tccaggtaca gcgggcgttg
     1141 aacgtatact ctttcgttta cctggcgtct ctgaccatga gatgaactgg aagcaatatc
     1201 tttgtgccat tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg
     1261 gtcagcacta tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc
     1321 tgaataccgc cgtcagcttt gtcacgaata ccaactggca atcttatagc ggtgaaacca
     1381 cgttgagcta tttcagccag atggcaggct taacggtaca aaactttctt tctgccgcca
     1441 gcgggattgc ggtgattttt gccttcatcc gtgcgtttac tcgccagagc atgagcacac
     1501 tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg ggtgctagtc cctgtggcgt
     1561 tgttgatcgc actgtttttt attcaacaag gtgcgctgca aaactttctg ccctatcagg
     1621 cagtgaatac cgttgaagga gcgcaacagc tgttacccat gggaccagta gcttctcagg
     1681 aagcgatcaa gatgctcggt actaacggcg gtggcttctt taatgccaac tcgtcgcatc
     1741 cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc
     1801 caacagcgct gtgctttgcc tttggtgaag tgacgggcga tcgccgccag gggcgcatgt
     1861 tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg cgtggttatg tgggcagaag
     1921 ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta
     1981 aagagagccg tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt
     2041 cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc tctcggcggc atggtgccga
     2101 tgtggctaat gcaaattggt gaagtggtgt tcggcggtgt cggttctggt ctttacggca
     2161 tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg
     2221 aatatctggg taaaaaaatc gacgtacgcg agatgaaact gattgcactg gcgattctgg
     2281 ttactccaac actggtactg atgggcgcgg cgttggcgat gatgaccgac gccggacgta
     2341 gcgccatgct taaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcgtccg
     2401 ccgctaacaa caacggcagc gcctttgccg gattaagcgc caactctccg ttctggaact
     2461 gtttactggc gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa
     2521 ttgccggttc gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc
     2581 acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct
     2641 ttatccctgc cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagcga
     2701 gcactgaata tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg
     2761 ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt
     2821 atcgtctgga tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggtgcg
     2881 atgcctggca atgcgctgtt tagcgcggca attagcggct ggctgtgggt caccgtactg
     2941 ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagtctt
     3001 aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg
     3061 gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca tcgtgctggt agaagctggc
     3121 gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga tgaaagcgcc
     3181 atcaccgggg aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc
     3241 ggcggcacgc gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggtgag
     3301 acatttctgg atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacaccgaac
     3361 gagattgccc tgactatcct gctgattgct ctgaccatcg tctttttact ggcaaccgcc
     3421 acgctgtggc cgttttccgc gtggggcggt aatgccgtga gtgtaacggt actggtggcg
     3481 ctgctggtct gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc
     3541 gggatgagtc ggatgctggg cgcgaatgtg attgccacca gcggacgtgc agttgaagcg
     3601 gcaggtgacg ttgacgttct gctactggat aaaaccggca ccatcacact cggtaaccgt
     3661 caggcgtcgg agtttatccc cgcgcagggc gtggatgaaa aaacgctggc tgacgccgca
     3721 caactggctt cgctggctga tgaaacgccg gaaggtcgca gtattgtgat cctcgccaag
     3781 caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt
     3841 actgcgcaaa gccggatgag cgggatcaac atcgacaacc gcatgatccg taaaggttct
     3901 gtcgatgcca ttcgccgcca tattgaggct aacggcggtc acttccctgc cgatgttgat
     3961 caaaaagtcg atcaggttgc gcgtcaggga gccacgccgc tggtggtggt ggaaggttct
     4021 cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag gcggtattaa agagcgcttc
     4081 gcccagctgc gcaaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact
     4141 gccgccgcga tagctgcgga agcgggtgtc gatgattttc tcgccgaagc aacaccggag
     4201 gccaagctgg cattgatccg tcagtatcag gcggaaggtc gtttggtagc gatgaccggc
     4261 gacggcacca acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc
     4321 ggcacccagg cggcgaaaga ggcgggcaat atggtggatc tcgactctaa cccgaccaag
     4381 ttgatcgagg tggtgcacat tggcaaacag atgctgatga cccgtggctc gctgaccacc
     4441 ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca
     4501 acatatccgc agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt
     4561 ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa
     4621 ggcgtgagtt ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac
     4681 ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctgacc
     4741 gtttgcggtc tggtgtgagg tttaccatga gtggattacg tccggcatta tcgacatttc
     4801 tctttctgtt attgattact ggcggcgttt acccgctgct gaccaccgca ctggggcaat
     4861 ggtggtttcc ctggcaggcc aacggttcgt tgatccgtga aggtgatacg gtgcgtggct
     4921 cggcattaat cgggcagaat tttaccggca acggctattt tcatggtcgc ccgtcagcaa
     4981 cggctgaaat gccctataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc
     5041 ctgagctgga taaactaata gccgcacgcg ttgctgcatt acgggccgct aacccggatg
     5101 ccagcgcgag cattccggtt gaactggtga cggcatcggc aagcgggctg gacaataata
     5161 tcaccccgca agcggcggcc tggcagatcc cacgcatcgc gaaagcgcgt aatctcagcg
     5221 ttgaacagct cacgcagctg atcgcaaaat acagccaaca accgctggtg aaatatatcg
     5281 gccagccggt cgtcaacatt gtcgaactca atctggcgct ggataagctt gatgaataac
     5341 gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg
     5401 aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga agacctgggc gatgctggca
     5461 gaagcccagc gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccac
     5521 gggcgaaaag ataccgccgc aatgctggaa gggctggctg ttctgccgcc aaaacgcctg
     5581 gcgtaccgtg ggcggcacat ctgcgagttt gacctcgatg ccgccctcgc ccgccgtccg
     5641 gcgctgatct taatggacga actggcgcac agtaatgcgc caggttctcg tcaccctaaa
     5701 cgctggcagg acatcgaaga gctgctggaa gcaggcattg atgttttcac taccgtcaac
     5761 gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg
     5821 gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg tggttctggt ggacttgccc
     5881 ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag
     5941 cgcgccattg aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga actggcactg
     6001 cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctagcgaa
     6061 gagaaagtct ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggtagt
     6121 gagaagctgg tccgcgcggc ggcgcggctg gcgtcacggc tgggtagcgt ctggcacgcg
     6181 gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg tgcaattctc
     6241 agcgccttac gtctggcgca ggaactgggc gcggagacgg caacactttc tgatccagcg
     6301 gaagagaaag cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt
     6361 cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc
     6421 gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac
     6481 aacgcgccgg atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg
     6541 gttgccgccg cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt
     6601 gatgccgcca acctggtgat gctgtatctg cttggcgtgg tggtggtggc gctattttat
     6661 ggacgctggc cttcggtggt tgccaccgtc attaatgtgg tgagtttcga tctctttttt
     6721 atcgccccac gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg
     6781 atgttaaccg tcgggctggt gatcgggaac cttactgctg gcgtgcgtta tcaggcgcgg
     6841 gtagcccgtt accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg
     6901 gtgggccgca gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt
     6961 catgcccgca gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat
     7021 ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg
     7081 cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt accagatttt gccgctaaaa
     7141 agcggcgaga aaacctacgg gctggtggtg gtggagccgg ggaatctgcg ccagttgatg
     7201 atcccggaac agcagcgcct gctggagacg tttacgctgt tagtcgccaa tgcccttgag
     7261 cggctgacgc taaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc
     7321 cgcaacgccc tgctggcagc gctttcgcat gatttacgca cgccgcttac ggtgctgttt
     7381 ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag
     7441 gccagcgaga ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat
     7501 atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa
     7561 gtagtcggca gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct
     7621 ctgccagaac cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt
     7681 aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc
     7741 cactttgagg gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca
     7801 ggccaggagc agacgatatt tgataagttt gctcgcggga ataaagagtc ggcggtgccg
     7861 ggtgtcgggc ttgggttggc aatttgtcgg gcgattgtgg atgtacacgg cggcaccatt
     7921 accgcgttca accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc
     7981 gctcctgaac ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg
     8041 aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctttg
     8101 aggccgaaac tctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca gatttgatta
     8161 ttctcgatct cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt
     8221 ggagcccggt gccggtgatt gtgctttccg cacgcagcga agagagcgac aagatcgccg
     8281 cgctggatgc cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg
     8341 cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga
     8401 aattttccga tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg
     8461 tgcatctcac accaattgag ttccgcctgc tggcggtgct gctcaacaat gccggaaaag
     8521 tactcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc
     8581 actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat ccggcccggc
     8641 ctcgccactt cattactgaa accggtattg gatatcggtt tatgctttga attgttattc
     8701 aataaattcg cttaaatagt gattaattta ttattaaacc tgcgccgaat attattattt
     8761 ggcaggttta att
//
LOCUS       DAQSWQ010000154         8632 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.156, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000154 DAQSWQ010000000
VERSION     DAQSWQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.156"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="R8G18_003879"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="R8G18_003879"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEE9797466.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="R8G18_003880"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="R8G18_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HEE9797467.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4608..5765
                     /gene="mdoC"
                     /locus_tag="R8G18_003881"
     CDS             4608..5765
                     /gene="mdoC"
                     /locus_tag="R8G18_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HEE9797468.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="R8G18_003882"
     CDS             complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="R8G18_003882"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HEE9797469.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="R8G18_003883"
     CDS             complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="R8G18_003883"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEE9797470.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7824..8135)
                     /locus_tag="R8G18_003884"
     CDS             complement(7824..8135)
                     /locus_tag="R8G18_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEE9797471.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="R8G18_003885"
     CDS             complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="R8G18_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HEE9797472.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2246 c         2051 g         2180 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaat agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg
     4261 cttatatcga tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt
     4321 atcgttcagc acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt
     4381 gcttctccta aagacagaag catcgacttc gtgtagggtt tgccagcaat tttatttatc
     4441 aaccgtctta cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac
     4501 cttgaagcct gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca
     4561 taacccgaca gggataagac gaaaaatccg agattagtta accatatatg aatccagtac
     4621 ccgcgcaacg tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc
     4681 cttttcatat ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccgt
     4741 cattgtggct gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg
     4801 ttatatccgg ctacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga
     4861 aagtacgtgt cgaacgtgta ggtatcccga tgttaacagc catcccctta ctgacattgc
     4921 cgcaatttat tatgctgcaa tacgtcaaag ggaaagcgga aagttggcct gggctgtcat
     4981 tgtatgacaa atataatacg ttggcctggg aattaatatc acacctgtgg tttttactgg
     5041 tgttagtagt catgacgacg ctgtgcgtat ggatatttaa acgcatcaga aataatttag
     5101 aaaattctga taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttat
     5161 gcctcggcat cggctatgcg gtaataagaa gaacgatttt tattgtgtat ccgcccattc
     5221 tgagtaatgg catgttcaat tttattgtca tgcaaacgct attttatttg ccgttcttta
     5281 tcctcggtgc actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc
     5341 gtggctgtac ccttgcagca gcattggcat ttgtcgctta tttactcaac cagcgctatg
     5401 gcagtggcga tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggtctgt
     5461 ggatggtgaa tgtggtcttc tcctttggcc accgtttgct taacttccag tcagcgcggg
     5521 tgacttactt tgttaacgca tcgctattta tctatctggt tcaccacccg ttaacgctgt
     5581 ttttcggcgc atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc
     5641 tgatattcgt agtagggatt gcgataattc tgtatgaaat tcatttacgc atcccgttac
     5701 tgaagttttt gttttctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc
     5761 gttaagccac atttacaata accattccac gggcaatatc gacgccagtc tgaccataac
     5821 ccgcttccag aaactggtgg cgggttcttt tttgagaata atctctttct ttgcatgacg
     5881 atcaacccag ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcatactg
     5941 gctctgaata aagcgtttat caattaactg tgccagcgtt tcgctctcta tcacgaagcc
     6001 catttcagta ttgagcaatg ttgaacgcgg atcgaaattg aaagagccga taaacaccgt
     6061 tttaccatcg atgctaaaag ttttagcatg caggctggct ccggaattac cggttatgcc
     6121 gcgatcgtgt aacgtactac tttgttcacg cgtcggcttg agttcatata attccacgcc
     6181 atagcggagc aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt
     6241 agcggcaaga gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag
     6301 ttgcgccaca cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact
     6361 gggtgagccc atgatatcgg acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc
     6421 ctcccctttc gccggatcat cacttaataa acgtgttttc gcccagataa gcggcaacgt
     6481 tccatcaacc agatgattta taaacggact ggattccatt tggcgtaagt aacgatgcgt
     6541 catggcatcg ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccttccgg
     6601 gacatccagc acctgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc
     6661 atcggcaacg tcctctacca cgggtcctat tgccataaca tctaaatccg aaaaaagtgg
     6721 ctcttcccct gccccaaaat aggcatcacc aatatttcgt cctcccacca gggtaaccac
     6781 gccatcgaca gtgaaacttt tattgtgcat acggcgattg agacgggaaa agtcggtgat
     6841 ataaccaagc ggacgcagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg
     6901 gggatggctg tcaagcaagc gtaaaatatc gtcaagtccg ggcgtattgt tgtcgtccag
     6961 caacaaacgg acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg
     7021 acccgacata tcgtcctgcc agatgtaata ctgaacatcc agcgtatgct ctgccatttc
     7081 ggccagacga taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca
     7141 ctgaccggga tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt
     7201 catctccttg ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga
     7261 caaagtatac ctgttcgggt aaagcgtgac gggtaataaa ttctgaaacg gttttaactg
     7321 cgatttcagc cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag
     7381 ccactgacgt atagctgttt gccgccacca gtcgcaggct attcagatag gcatcctgca
     7441 aaagctggtc ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgtactacgg
     7501 ctttagcggg aagatcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct
     7561 gctgttgcct gacttttaaa caggcatcca gcagggccgg acccgctgcg cgatgaatgg
     7621 ccccatcgac gccgccgcct cccattaatg acggattagc cgcattcaca atcacatcaa
     7681 cggccagttt ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt
     7741 acaccttctt gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc
     7801 agcaacgtgc gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc
     7861 tgtttatcaa gatactgcac ttttactgag gctatttgcg cgtttttaac attaccgctc
     7921 attaacgctt tgctggaata ggttttgccc ggaacagaac cattctgtgg gcagttcatt
     7981 tctacgggtg atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa
     8041 tggatcacac ccccttgcgc ggtgcttcct gcgatggcaa taaaaggaaa aaacaaactg
     8101 ccgagcataa gagagtttaa aaaaggtctg aacatttatt ctatttcctc aatgattagt
     8161 catccttgag ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct
     8221 ctccagattc atcttatgct cgatatttca acaaattaag acttttctga agagggcggc
     8281 cattgttgtg ataaatgaag tgcctgtcca tcagaaacag taacaactat tttcacccga
     8341 tcttccgggg aaatatttaa actcaacttc gtcaaagcaa taggttgatt agcaggcaat
     8401 gagagggtct tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat
     8461 atttgtactc tgcacagaca agattgagta agagtgactt caggaataat ggtatacaca
     8521 tccccttgct gggtcgtatt aaaggttatc tggctggaaa gtgccgcaag gagtaataac
     8581 gcattcatat tcttctcccg aaaaaaacag ggcttgcgcc ctgtttctgt aa
//
LOCUS       DAQSWQ010000155         8214 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.157, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000155 DAQSWQ010000000
VERSION     DAQSWQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.157"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="R8G18_003886"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="R8G18_003886"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HEE9797473.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="R8G18_003887"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="R8G18_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HEE9797474.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="R8G18_003888"
     CDS             2028..2546
                     /locus_tag="R8G18_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEE9797475.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="R8G18_003889"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="R8G18_003889"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HEE9797476.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="R8G18_003890"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="R8G18_003890"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HEE9797477.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="R8G18_003891"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="R8G18_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HEE9797478.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="R8G18_003892"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="R8G18_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HEE9797479.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="R8G18_003893"
     CDS             complement(6710..8170)
                     /locus_tag="R8G18_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEE9797480.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2170 a         2055 c         1871 g         2118 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaagc tatatgtttt gtta
//
LOCUS       DAQSWQ010000156         8182 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.158, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000156 DAQSWQ010000000
VERSION     DAQSWQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.158"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="R8G18_003894"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="R8G18_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HEE9797481.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="R8G18_003895"
     CDS             complement(2975..3697)
                     /locus_tag="R8G18_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HEE9797482.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="R8G18_003896"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="R8G18_003896"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HEE9797483.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="R8G18_003897"
     CDS             6679..>8182
                     /locus_tag="R8G18_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HEE9797484.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2321 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAQSWQ010000157         8157 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.159, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000157 DAQSWQ010000000
VERSION     DAQSWQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.159"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..254
                     /locus_tag="R8G18_003898"
     CDS             <1..254
                     /locus_tag="R8G18_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HEE9797485.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="R8G18_003899"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="R8G18_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HEE9797486.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="R8G18_003900"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="R8G18_003900"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HEE9797487.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="R8G18_003901"
     CDS             complement(2905..3180)
                     /locus_tag="R8G18_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEE9797488.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="R8G18_003902"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="R8G18_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEE9797489.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="R8G18_003903"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="R8G18_003903"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEE9797490.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="R8G18_003904"
     CDS             5140..5466
                     /locus_tag="R8G18_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEE9797491.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="R8G18_003905"
     CDS             5650..6369
                     /locus_tag="R8G18_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HEE9797492.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="R8G18_003906"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="R8G18_003906"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HEE9797493.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8157
                     /locus_tag="R8G18_003907"
     CDS             7708..>8157
                     /locus_tag="R8G18_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HEE9797494.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2087 a         1988 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattat
//
LOCUS       DAQSWQ010000158         8054 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.160, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000158 DAQSWQ010000000
VERSION     DAQSWQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.160"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            103..468
                     /locus_tag="R8G18_003908"
                     /pseudo
     CDS             103..468
                     /locus_tag="R8G18_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
     gene            complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="R8G18_003909"
     CDS             complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="R8G18_003909"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEE9797495.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3236..4069
                     /gene="ppsR"
                     /locus_tag="R8G18_003910"
     CDS             3236..4069
                     /gene="ppsR"
                     /locus_tag="R8G18_003910"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HEE9797496.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4226..5272
                     /gene="aroH"
                     /locus_tag="R8G18_003911"
     CDS             4226..5272
                     /gene="aroH"
                     /locus_tag="R8G18_003911"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HEE9797497.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5404..5595
                     /gene="hemP"
                     /locus_tag="R8G18_003912"
     CDS             5404..5595
                     /gene="hemP"
                     /locus_tag="R8G18_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEE9797498.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5599..7035)
                     /locus_tag="R8G18_003913"
     CDS             complement(5599..7035)
                     /locus_tag="R8G18_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEE9797499.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="R8G18_003914"
     CDS             complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="R8G18_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HEE9797500.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2073 a         2113 c         1910 g         1958 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccaaaattac cggacgcaaa
      121 aaagatatta ttgtccgcgg cggcgaaaat attagcagcc gtgaagtaga agatatttta
      181 ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa tgcccgatga acgtttaggt
      241 gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc attcattatc gctggaagag
      301 gtggtggctt tttttagccg taaacgggtc gcaaaatata aatatcctga acatatcgtg
      361 gtaatcgaaa aactaccgcg tactgcctca ggtaaaatac aaaagttttt gttaagaaaa
      421 gatattatgc ggcggttaac tcaggatgtc tgtgaagaga ttgaataagt ttcatcttcg
      481 gggatcacat aaccccggcg actgaacgcc gccggggatt tattttattt cttcagttca
      541 gccaagctta accaggtttg caccacggtg tccgggttca gagacaggct atcgatcccc
      601 tcttccatca accatgcggc aaagtcttcg tggtcggacg gaccctgacc gcaaatcccg
      661 acatatttgc cctgtttctt cgcggcacgg atagccatcg acagcagtgc tttcaccgca
      721 tcgttgcgct catcgaacaa ttcagacacc acgccggagt cacggtccag accgagcgcc
      781 agctgcgtca tatcgtttga gccaattgag aagccgtcga aatattcgag gaactgctcg
      841 gccagcaagg cgttggacgg gatttcacac atcatgatga ttttcagccc gttctcacca
      901 cgcttcagcc cctgacgcgc cagttcctca accaccgctt tcgcctgatc tacggtacgc
      961 acgaacggga tcatgatctc aacgttggtc agccccatgt cgttgcgcac acgtttcact
     1021 gcttcgcact ccagcgcgaa gcagtcgcgg aagctgtcgg aaacatagcg tcccgcgcca
     1081 cggaagccga gcatcgggtt ctcttcatct ggctcgtaac gctcaccacc gaccaggttg
     1141 gcatattcgt tcgatttaaa atcagagaga cggacaatga cgcgcttcgg ataaaacgcg
     1201 gcacccagcg tcgcgatccc ttcagtcaga cgaccaacgt aaaattcacg cggagaatca
     1261 aaacctttca tcatctcgcg gatttcattt tgcaactgcg gttcctgatc gtcaaactca
     1321 agcagtgcgc gtgggtggac gccaatcata cggttgatga taaattccag acgcgcaagg
     1381 cccacgcctt cgttcggcag gcaggcgaag tcgaaagcac ggtccgggtt accgacgttc
     1441 atcatcactt tcaacggcag atccggcatc atttctacgc tggagctttt cacgctaaat
     1501 tccagcaact ccgcatagac gtaaccggta tcaccttcgg cacaagaaac agtgacgttc
     1561 tcaccgtctt tcatccgttc tgttgcatca ccacagccca ctaccgccgg aatgcccagt
     1621 tcacgagcga tgatcgccgc gtgacaggta cgaccgccac ggttggtgac gatggcagat
     1681 gctttcttca tgatcggttc ccagtccggg tcggtcatgt cagtgaccag cacgtcacca
     1741 ggttcgatgc ggttcatttc gctgatctca tggatgactt tcaccggacc cgcgccgatg
     1801 cgatgaccga tagcacggcc ttcggcgata atcttaccct gtgaatgcag cgtataacgc
     1861 tccatgacct gaccgcgtga gcgcacggtt tccggacgcg cctgcacaat gaacagtttg
     1921 ccagtgtggc catctttcgc ccactcaata tccatcgggc gaccgtagtg tttctcaatt
     1981 tgtacggcct gttttgccag ttcctgcact tcttcgttgg tcagcgagaa gatgtcacgc
     2041 tgttcctgcg gtacgtcttc gattttaacc tgcttgccgt gctcctgggt cggcgcgtaa
     2101 accatgcgga tttttttcga ccccatggtg cgacgcacga tagccgggcg attcgccgcc
     2161 agtgtcggtt tatggacgta aaactcatcc gggttaaccg caccctgcac gaccatttcc
     2221 ccaaggcccc atgcggaagt gataaacacc acctggtcaa agccggattc ggtatcaatg
     2281 gagaacatca cgccagaaga tgcgaggtcg gagcgcacca tccgctgaac accggcagag
     2341 agcgccacgc cacggtgatc gtaaccctgg tgcacacgat aagagatggc gcgatcgtta
     2401 aacagagaag caaatacatg cttcactgcc acgagaacgg cgtcaaaacc ctgaacgttg
     2461 aggaaggttt cctgctgacc ggcaaaagaa gcgtccggca tatcttctgc ggtggcggag
     2521 gagcgcaccg caaaagaggc gttttcgtca tcggcggaaa gctgtgcata ggcttcgcgg
     2581 atggcgtttt ccagctcagg ctggaaggga gtgtcgataa tccactggcg gatttgcgcg
     2641 cccgctttcg caagctgagt aacatcgtca atatccgttt tatccagcag ttcataaatg
     2701 cgctggttta cgccgctttg gtccagaaac tggttaaacg cgtcggcggt tgtggcgaaa
     2761 ccattcggaa cggaaacacc cattccggaa aggttagtaa tcatttcacc cagggaggca
     2821 tttttgcccc caaccctgtc tacatcattc atgccgagtt ggttatacca aagcaccagc
     2881 ggtgacgagc cattgttgga catcgaacaa tccttttgtg ataaatgaac ggtttgagaa
     2941 acacatttct gcgcatttat ctttgcatat ttaaccggat gaaaaaaacg gtgaatcgtt
     3001 caagcaaata tattttttta ctttttaaga ctgatcccag cgttgcgcaa atctgctctc
     3061 ttcgacaatt tccacataaa ccatcggtat aaacgaaacc ataaaaatga aatgctgttt
     3121 tcataaaaaa taaaattgaa ggttcatttt ataaaccaga cataacgtcc tacgcttctg
     3181 tgcgttttta atttatgctt tcatagaatt atgtctgcat cacgggaaga acaaaatgga
     3241 taatgctgtt gatcgccacg ttttttatat ttctgatggt acggcaataa ctgcggaggt
     3301 attaggacac gcagtaatgt cacaatttcc cgtcactatc agcagcatta cgctgccgtt
     3361 tgtcgaaaat gagagccgtg cacgggcagt gaaggatcag attgacgcaa tttatcacca
     3421 gacaggtgtg cgcccgctgg tcttctactc catcgtgttg ccggagattc gcgccatcat
     3481 cttgcaaagt gaaggctttt gccaggatat cgttcaggcg ctggttgccc cgctacaaca
     3541 agagatgaaa ctggatccaa cgccgattgc tcatcgtacc catggcctta accctaataa
     3601 tctcaataaa tatgatgcgc gcattgcggc gattgattac accctcgccc acgatgacgg
     3661 catttcgttg cgcaatctgg atcaggctca ggtgatcctg ctcggtgttt ctcgctgtgg
     3721 taaaaccccc accagtcttt atctggcaat gcaatttggt atccgcgcgg caaactaccc
     3781 ctttattgcc gacgatatgg ataatctggt gctacccgcg tcgctcaaac cgcttcagca
     3841 taaattgttc ggcctgacta tcgacccgga acgtctggcg gcgattcgcg aggaacgtcg
     3901 ggagaacagt cgctatgcct cgcttcgtca gtgcaggatg gaagtcgcgg aagtggaagc
     3961 gttgtaccgt aaaaatcaga tcccgtggat taacagtacc aattattcgg tagaagagat
     4021 tgccaccaag atcctcgata tcatgggcct tagtcgccga atgtactaga gaactagtgc
     4081 attagcttat ttttttgtta tgatgctaac cacccggcga ggtgtgacac acctcgcact
     4141 tgaaatcagc agcgattggt ttatcgtgat gcgcatcact tcccggcagt cctgccgtag
     4201 aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta ctgcgcgtat
     4261 tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc ctggcgccgc
     4321 cacccatgtc accgactccc gccgcagaat tgaaaaaata ctcaatggtg aagataagcg
     4381 actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa tggagtacgc
     4441 cccccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg taatgcgcac
     4501 ctattttgaa aaaccacgaa ctgttgtcgg ctggaaagga ctaatctccg atccagattt
     4561 aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac ttttacaggt
     4621 aaatgagctg ggcgtcccaa ccgcgactga gttcctcgat atggtgaccg gtcagtttat
     4681 tgctgattta atcagttggg gcgcgattgg cgcacgtact accgaaagtc agatccaccg
     4741 tgaaatggct tcggcactct cctgtccggt aggttttaaa aatggtaccg atggcaatac
     4801 gcggattgct gtggatgcta tccgcgcagc ccgcgccagc catatgttcc tctcgccaga
     4861 caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc acattattat
     4921 gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct gcgatacgct
     4981 gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta actgccagaa
     5041 gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc gcaatggctc
     5101 tacggcgatt gctggaatta tggcggaaag tttcctgctc gaaggaacgc aaaaaatcgt
     5161 cggcagtcag ccgctcactt acggtcaatc cattaccgac ccgtgtctgg gctgggaaga
     5221 taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat acccgcttct gaatgcgtgc
     5281 ccattcctga cggaatgggc atttctgcgc aacttgttgt cttctcaaca aattactgct
     5341 tgctctggcc tgccataata ttgataataa gaatcattgt tatatcaatt attattaatc
     5401 tttatgcgtt atacggatag cagaaaactc acgcctgaaa cggacgccaa tcacaaggcc
     5461 gcttccccgc agcccatccg gcgaattacc agccagacac tgttaggtcc ggatggcaaa
     5521 ctgattatcg atcatgacgg gcaagaatat ctgctccgta aaactcaggc tggcaagctg
     5581 ctgttaacta agtagccttt aactcgagca gctgacttcc agccgtttac cccagtcagg
     5641 tggacgactg acatagtcat catctctatc gctgaaagga tttcgcaacg ccccatgcag
     5701 gcggtgcaat tccgtcatat cacccttttc tgccgcttca atcgcccgtt gcgccaacca
     5761 gttgcgcaat accagagcgg ggttaacgct ttgcatcaac tgctgacgct cactatcgct
     5821 aacctcgtct tgttgcaaac gcccccgata acgggcaaac cagccatcaa atgccgcacg
     5881 atcaataaac tcatcacgta gcggtgacgc cgtgctgtgc tgctcggtca gactcagcat
     5941 gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc agactgaata attcattcag
     6001 tagcgcgtta tcctcttttt gctccgtgat gaagcccagt ttctgccgca tccgttgtcc
     6061 ataatgcgtc aacaaaacct gctgatagct gtccagggcc tcattcaggg catctacggc
     6121 aacaaatggt gacaatgtct gcgccagacg ctgtaaattc cacaacgcga cagcaggttg
     6181 attatcaaaa ctataacgcc cttgatgatc cgagtgatta caaataaaac cgggttcgta
     6241 atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg acatgttgtc
     6301 ggtattcatc accccatgtg caaagccgac cgtctgccat tgggcaatta acgacgcggt
     6361 acgtgcgaca acatcgctaa accagagacg gtatttgtcc tcatcatctt caagatgtga
     6421 ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc ttttccgact cgcggcggta
     6481 gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt gccacacgca tcagcatcgc
     6541 gcctggctcc gccgtttccc gatacactgg cgaatcgctg gtgacgatac ttaacgcgcg
     6601 ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg agactttctc gtatcgtcga
     6661 acgtaaaacc gcccgtccat cacccattcg cgaataaggc gtcaggccag cacctttcag
     6721 atgccagtcc attgtagtgc catcagcaag cagttgttcg ccgagtaaaa tgccgcgccc
     6781 atcacccagt tggcccgccc agacgccgaa ctgatgacca ctgtaaacct gcgccagtgg
     6841 tgacatacca ggcagtaggg cttcgccgcc ccagacacct gcgccatttt taaacagcga
     6901 cgatggaata ctcagcgtgt tagccagttc ggtattatgc caaatcagcc gggcattatt
     6961 taaaggcgta ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc agcgggtaac
     7021 aaaagacagg gtcatagatc ctcctgtttg atagtgtaga cggttactct cgtcttaaac
     7081 accagcaaac agaagggtta tcgctgaacc aacgtcgtta tctgatttat gggaacggca
     7141 ggccacaggc atccctggag agcatggaaa tcgaaggggg taatctgtgc caatatttct
     7201 gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg cgcctggatc
     7261 gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa acttttatcc
     7321 agcatgacgc gggtgaaaag accatcaaaa accgctttca ttgtgctatt gcctgctccc
     7381 aaattaccta acacaagggg atacacttgc gataaggata aaagaccgcg attatctttt
     7441 ccttcattga gatgcgggta attctcatta atcaacagtt caataaacgg atattttaat
     7501 agttcgcctg cgtaattatc cctctctaat aataatgtag ccacttgtgg cgttaaatta
     7561 agccaggcaa agagtttatg ttgaataaaa aaatgttggc atgattttag taattccaac
     7621 tgctctgaaa ataattgcca atgttgctcc tcggtaagtt gcgctataac ccgactcgtc
     7681 gggatacgca ccgtgccatc ttcactggag aaatgggtaa ttaactcaac gccgacaagg
     7741 acttgctggt tatctcttat cggcaggaaa tagcaatcag aatgataaag attctccaaa
     7801 aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg ctaaaaggga
     7861 tgaggccata agacataaca attacagaag gagtaacttt catttgttcc atgttaacca
     7921 ctttttcagg ggtccttttt ttagattatc ctgattataa acgaataatc catttacgga
     7981 atttttgtct gcaaaatact actgtattca ggagtaaatg gacggcgatg tatcatcgcc
     8041 gtccattttt cgac
//
LOCUS       DAQSWQ010000159         7777 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.161, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000159 DAQSWQ010000000
VERSION     DAQSWQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.161"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1780)
                     /locus_tag="R8G18_003915"
     CDS             complement(<1..1780)
                     /locus_tag="R8G18_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEE9797501.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="R8G18_003916"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="R8G18_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HEE9797502.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="R8G18_003917"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="R8G18_003917"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEE9797503.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="R8G18_003918"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="R8G18_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HEE9797504.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="R8G18_003919"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="R8G18_003919"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HEE9797505.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="R8G18_003920"
     CDS             complement(5968..7005)
                     /locus_tag="R8G18_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEE9797506.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7777
                     /gene="yhhY"
                     /locus_tag="R8G18_003921"
     CDS             7339..>7777
                     /gene="yhhY"
                     /locus_tag="R8G18_003921"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEE9797507.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2102 c         1872 g         1826 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gattgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacgcat
//
LOCUS       DAQSWQ010000160         7716 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.162, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000160 DAQSWQ010000000
VERSION     DAQSWQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.162"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..644
                     /locus_tag="R8G18_003922"
     CDS             <1..644
                     /locus_tag="R8G18_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEE9797508.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            895..2457
                     /gene="hpaB"
                     /locus_tag="R8G18_003923"
     CDS             895..2457
                     /gene="hpaB"
                     /locus_tag="R8G18_003923"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HEE9797509.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2475..2987
                     /locus_tag="R8G18_003924"
     CDS             2475..2987
                     /locus_tag="R8G18_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HEE9797510.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3380..5530
                     /gene="btsT"
                     /locus_tag="R8G18_003925"
     CDS             3380..5530
                     /gene="btsT"
                     /locus_tag="R8G18_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HEE9797511.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5580..5783
                     /locus_tag="R8G18_003926"
     CDS             5580..5783
                     /locus_tag="R8G18_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEE9797512.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5794..6750
                     /gene="yjiA"
                     /locus_tag="R8G18_003927"
     CDS             5794..6750
                     /gene="yjiA"
                     /locus_tag="R8G18_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEE9797513.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6828..>7716)
                     /locus_tag="R8G18_003928"
     CDS             complement(6828..>7716)
                     /locus_tag="R8G18_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEE9797514.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1783 a         2104 c         2021 g         1808 t
ORIGIN      
        1 ctcatgcgtt tattacggag tctgacgccg acggtcatgt gttgacggta cgggaagatc
       61 tgatctggcc cctgctggaa gttctttatc caggcactcg ggaaaccttc ggcctgccgg
      121 ggatttgcct gtcactggca gataaacccg acgaactggc ggcgctggaa cactattggc
      181 aactgataga gcgggaatcg gtggaacaac tgcctggacg ggaacacacc ctgacgctac
      241 tggcacaggc agtgttcacc ctactgctgc gtaatgcaaa actcgacgac catgccgcca
      301 gcgggatgcg cggagaatta aaactgttcc agcgttttaa tatgctgatt gaaagccatt
      361 ttcatcagca ctggacagta ccggattacg ctaacgaact gcatatcacc gaatcacgcc
      421 tcacggacat ctgccgccgc tttgccaacc gtccgccaaa acggttgatt ttcgacaggc
      481 agctgcgaga agccaagcgg ctgctgctgt tttctgataa cgccgtgaac aatattgcct
      541 ggcaactcgg ttttaaggat ccagcttatt ttgcgcgctt ttttaatcgc ttagtcggtt
      601 gctcgcccag tgcttatcgt gccaaaaaag tacctgtgac gtgaaaatcc cttagcctta
      661 acgggaaacc aggcaccacc tgctattccc ctttctccgc ctggagcaag gggatttagt
      721 aacgtcaaaa atcgctaaaa gcgaccgcga tcacaaaacc gtacccgacc tgaaaagtac
      781 cagccattgt cccaaaagtg tcttcccgta atcgcccctg caagcttcaa ttgaataaca
      841 aaaacaacac acaaaattaa caatcgtatt ccgattaata ctgtagaggt cgacatgaaa
      901 ccagaagatt tccgcgccag tacccaacgt ccgttcaccg gggaagagta tctgaaaagc
      961 ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag tgaaagacgt cactactcat
     1021 ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt acgacgcgct gcacaaaccg
     1081 gagatgcagg actctctgtg ctggaacacc gacaccggca gcggcggcta tacccataaa
     1141 ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc aacgtgacgc catcgctgag
     1201 tggtcacgcc tgagctatgg ctggatgggc cgtaccccag actacaaagc tgctttcggt
     1261 tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg agcagaacgc ccgtaactgg
     1321 tacacccgta ttcaggaaac tggcctctac tttaaccacg cgattgttaa cccaccgatc
     1381 gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca tcaagctgga aaaagagact
     1441 gacgccggga ttatcgttag cggtgcgaaa gtggttgcca ccaactcggc gctgactcac
     1501 tacaacatga ttggcttcgg ctcggcacaa gtaatgggcg aaaacccgga cttcgcactg
     1561 atgttcgttg cgccaatgga tgccaatggc gtcaaattaa tctcccgcgc ctcttatgag
     1621 atggtcgcgg gtgctaccgg ctcgccgtac gactacccgc tctccagccg cttcgatgag
     1681 aacgatgcga ttctggtgat ggataacgtg ctgatcccat gggaaaacgt actgatctac
     1741 cgcgatttcg atcgctgccg tcgctggaca atggaaggcg gttttgcccg tatgtatccg
     1801 ctgcaagcct gtgtgcgcct ggcagtgaaa ctcgacttca ttacagcact gctgaaaaaa
     1861 tcactcgaat gtaccggcac cctggagttc cgtggtgtac aggccgatct cggtgaagtg
     1921 gtggcgtggc gcaacacctt ctgggcattg agtgactcga tgtgttctga agcgacgccg
     1981 tgggtcaacg gggcttattt accggatcat gccgcactgc aaacctatcg cgtactggca
     2041 ccaatggcct acgcgaagat caaaaacatt atcgaacgca acgttaccag tggcctgatc
     2101 tatctccctt ccagtgcccg tgacctgaac aatccgcaga tcgaccagta tctggcgaag
     2161 tatgtgcgcg gttcgaatgg tatggatcac gtccagcgca tcaagatcct caaactgatg
     2221 tgggacgcca ttggcagcga gtttggtggt cgccacgaac tgtatgaaat caactactcc
     2281 ggtagccagg atgagattcg cctacagtgt ctgcgccagg cacaaagctc cggcaatatg
     2341 gacaagatga tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa cggctggact
     2401 gtgccgcact tgcacaacaa cgacgatatc aacatgctgg ataagctgct gaaataacgc
     2461 agcaggaggt taagatgcaa ttagatgaac agcgcctgcg ctttcgtgac gcgatggcca
     2521 gcctgtcggc agcggtaaat attatcacca ccgagggcga cgccggacaa tgcgggatta
     2581 cggcaacggc cgtctgctcg gtcacggata caccaccatc gctgatggtg tgcattaacg
     2641 ccaacagtgc gatgaacccg gtttttcagg gcaacggtaa gttgtgcgtc aacgtcctca
     2701 accatgagca ggaactgatg gcacgccact tcgcgggcat gacaggcatg gcgatggaag
     2761 agcgttttag cctctcatgc tggcaaaaag gtccgctggc gcagccggtg ctgaaaggtt
     2821 cgctggccag tcttgaaggt gagatccgcg atgtgcaggc aattggcaca catctggtgt
     2881 atctggtgga gattaaaaac atcatcctca gtgcagaagg tcacggactt atctacttta
     2941 aacgccgttt ccatccggtg atgctggaaa tggaagctgc gatttaagct ccagcccctt
     3001 cacgcattcg tcgaaggggc atttttattg tgatctcaat cacatatcca cccgatgaat
     3061 aattcttgcc caaccgctca ccgccattcc tgaccgctta cgtccctaaa ataaccactc
     3121 agttatttac cttactttac gcgcacgtaa ctctggcaac atcactgcag gatagcggtc
     3181 aatttacctc ctcaaacgca acgcaaacct agaacggctt cggcctatga ttaacaaatt
     3241 actctatgaa ttttggtttg caggaaggca gcaagtgagt gaatccccgg gagcttacaa
     3301 cagtaagtga caggggtgaa cgaacgcaac tgccgcacct gtaagccaaa agacgacgag
     3361 taaaatgcca ggttttacta tggatacgaa aaaactattc aagcacatac cctgggtgat
     3421 tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt
     3481 cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta
     3541 cagtctgtac atcgcccaga aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt
     3601 tattaacaac gacggtctga actacgttcc gaccaaccgt tacgtgttgt ttggtcacca
     3661 cttcgccgct atcgccggtg ctggtccgct ggtcggtccg gttctcgccg cacagatggg
     3721 ccacctgcct ggcacgctgt ggctgctggc gggggtagta ctggccggtg cggttcagga
     3781 ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg agatgataaa
     3841 agaagagatg ggaccagtac cggggactat cgcgctgttt ggctgtttct taatcatgat
     3901 catcatcctt gccgtcctgg cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg
     3961 tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct acatgcgctt
     4021 tatccgtccg gggcgtgtgg gtgaagtctc tgtcattggt atcgtgctgc tggttgcctc
     4081 tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac tgacctttaa
     4141 agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcat tgctgccggt
     4201 gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt
     4261 cggcctggcg ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg cgatgaccca
     4321 gtacattgac ggtactggcc cgctgtggaa aggtgctctg ttcccgttct tgttcattac
     4381 catcgcctgt ggtgcggtat ctggcttcca cgcactgatc tcttccggta cgacgccgaa
     4441 actgctggct aatgaaaccg acgcgcgttt cattggctac ggcgcaatgc tgatggagtc
     4501 cttcgtggcg attatggcgc tggttgctgc gtccatcatc gaaccgggtc tttacttcgc
     4561 gatgaacacc ccgcctgctg gccttggcat caccatgcct aacctgcatg aaatgggtgg
     4621 cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt accgcacacg cggcagcgac
     4681 cgtcagctct tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat
     4741 tggtgagcct tctgtcctga accgtgcagg tggtgcgcca acgctggcgg taggtatcgc
     4801 tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc acttcggtat
     4861 tctgttcgaa gccctgttca tcctgaccgc gctggatgcg ggtacccgtt ctggccgttt
     4921 tatgctgcaa gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg actctctggt
     4981 tgccggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg
     5041 cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta tctccaacca
     5101 gatgctggca gccgtagcgc tggtactggg caccgttgtg ctgattaaga tgaagcgcac
     5161 ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca ccacctgggc
     5221 gctgggtctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct acatggcaag
     5281 ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcgcagcaga ttgccaacat
     5341 gaaccacatc gttgtgaaca actacaccaa cgcaggcctg agtattctgt tcctgattgt
     5401 ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta acagcgacaa
     5461 acgtactgac aaagaaactc cgtacgttcc aatcccggaa ggcggcgtga agatctcttc
     5521 gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt gttcttatca gagtgaacta
     5581 tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag atgttgattg
     5641 gcattccaga ctacgacaac tacgtcgagc atatgaagac caaccatccc gacaagccgt
     5701 acatgagcta tgaagaattc ttccgcgaac gccagaacgc gcgctacggc ggcgatggta
     5761 aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt taccctactc
     5821 accggttttt taggcgcggg caaaaccacc ctgctgcgcc atattcttaa cgaacaacac
     5881 ggctacgaga ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg
     5941 attggcgacc gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc
     6001 tccaacgagc tggaagacgc actactcgac ctgctggata atctcgacaa gggcaatatt
     6061 cagttcgacc gtctggtcat tgaatgcacc ggcatggccg atcccggccc gattattcag
     6121 accttcttct cccatgagat tttatgccag cgctacctgc tggacggggt gattgcgctg
     6181 gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca gtcccaggtt
     6241 ggctacgccg accgtattct gctgacgaaa accgacgtcg caggcgaagc agagaaactg
     6301 cgtgaacgcc tggcgcgcat caacgcccgc gcaccggtct acaccgttac ccacggcgat
     6361 atcgacctgg gccttctgtt caacaccaac ggttttatgc tggaagaaaa cgtcgtcagc
     6421 accaaaccgc gtttccactt tatcgcggac aaacaaaacg atatttcgtc gattgtggtg
     6481 gaactggatt acccggtaga tatcagcgaa gtttcccggg tgatggaaaa cctgctgctg
     6541 gaatcggcgg ataaactgct gcgttacaaa gggatgctgt ggattgacgg cgaacctaac
     6601 cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg actgggaccg cccgtggggc
     6661 gatgaaaaac cgcatagcac gatggtgttt atcgggattc agttgccgga ggatgagatt
     6721 cgggcggcgt ttgcgggact caaaaagtaa ccagccctcc aacatcacaa tacctgagac
     6781 tttatcaccc tcatattcct ccagaatatg agggcattcg ggcttaatta tctctgaacg
     6841 tgcaacactt cctgcgactc cttatgcgcc tccacatcct gcttcgttaa ccagagcgac
     6901 ttacgaccaa aattttcgta cattttcagc tgatcttcat agtgctgatc aactgttcca
     6961 tcgggagcaa taaacccact ctgaccgggt gcgaccacat cccaggcaag caccggacga
     7021 tcgcttgtcg ttggtgagaa aacaatcata tcgttttctg ttccacggtt ttgatactcc
     7081 gcctgatgac gcgtttcttc cgctgcggcc tgcggtacac caaagaaatt atttgcacgg
     7141 aacgttaagg ccattgcagg tgttttccag ttactcacat tattgccata gcgtttggaa
     7201 agagtctccc aggtatcttc cagcgcagcc aacacaacct cctgctgtgg tttcccagca
     7261 aacagatcaa ccgcctgtgg gattggtgat ttgtctccct gcaccgcctc atacaaaatt
     7321 tttgctccaa cacttatatt cagcgaacca gttgggccgt cctgggttgt ttcgtagcca
     7381 ctggcgctgt accacttatc aaatggcata ggtacggcag ccactacggt acgcttcaac
     7441 atactggtca gccaaacgtt caggatggca gagcctggct gctgccaggt tttaccatca
     7501 tcattaagca aattgatgcc atcccaacgt gttaatgtat ccaccaactg acggcgcgga
     7561 tcgctctgtg tcaaaccaga tgtcgctgct tgcagagtag gtaaaaaaag cctcaggtta
     7621 agatcctgac gactggtttg gcgaataaca tcccatgcct gatcagcagt taagcgtggc
     7681 ttttgctcaa gcagtcggtc gatctccgta acgcga
//
LOCUS       DAQSWQ010000161         7611 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.163, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000161 DAQSWQ010000000
VERSION     DAQSWQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.163"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            171..701
                     /locus_tag="R8G18_003929"
     CDS             171..701
                     /locus_tag="R8G18_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HEE9797515.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            780..1502
                     /locus_tag="R8G18_003930"
     CDS             780..1502
                     /locus_tag="R8G18_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797516.1"
                     /translation="MVNNIANHSSFPAVTIDSQMLLKMVNEARKLYGEPSVRNNKFIE
                     KIEDELEGETYTKSVGRKNGADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEA
                     HIKSGKSASGLVEYRQARTLKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNA
                     IPLPAIEEHYYSAGEVAEQLGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVET
                     FRYNEAGVHKIEELIEGERKAA"
     gene            1824..2447
                     /locus_tag="R8G18_003931"
     CDS             1824..2447
                     /locus_tag="R8G18_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HEE9797517.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="R8G18_003932"
     CDS             2532..2750
                     /locus_tag="R8G18_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEE9797518.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="R8G18_003933"
     CDS             2728..3798
                     /locus_tag="R8G18_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEE9797519.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="R8G18_003934"
     CDS             3933..5216
                     /locus_tag="R8G18_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HEE9797520.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="R8G18_003935"
     CDS             complement(5450..>7611)
                     /locus_tag="R8G18_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HEE9797521.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAQSWQ010000162         7491 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.164, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000162 DAQSWQ010000000
VERSION     DAQSWQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.164"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="R8G18_003936"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="R8G18_003936"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEE9797522.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="R8G18_003937"
     tRNA            647..722
                     /locus_tag="R8G18_003937"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="R8G18_003938"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="R8G18_003938"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HEE9797523.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="R8G18_003939"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="R8G18_003939"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HEE9797524.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="R8G18_003940"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="R8G18_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HEE9797525.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="R8G18_003941"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="R8G18_003941"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HEE9797526.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="R8G18_003942"
     CDS             complement(5917..6249)
                     /locus_tag="R8G18_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEE9797527.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="R8G18_003943"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="R8G18_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HEE9797528.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1824 a         2031 c         1868 g         1768 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgattta tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAQSWQ010000163         7121 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.165, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000163 DAQSWQ010000000
VERSION     DAQSWQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.165"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            4..435
                     /locus_tag="R8G18_003944"
     CDS             4..435
                     /locus_tag="R8G18_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEE9797529.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="R8G18_003945"
     CDS             518..1420
                     /locus_tag="R8G18_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEE9797530.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="R8G18_003946"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="R8G18_003946"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEE9797531.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="R8G18_003947"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="R8G18_003947"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HEE9797532.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="R8G18_003948"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="R8G18_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEE9797533.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="R8G18_003949"
     CDS             complement(5330..6037)
                     /locus_tag="R8G18_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HEE9797534.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="R8G18_003950"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="R8G18_003950"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEE9797535.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="R8G18_003951"
     CDS             complement(6895..7098)
                     /locus_tag="R8G18_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEE9797536.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1639 a         1990 c         1855 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggacgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAQSWQ010000164         7064 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.166, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000164 DAQSWQ010000000
VERSION     DAQSWQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.166"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..87
                     /locus_tag="R8G18_003952"
     CDS             <1..87
                     /locus_tag="R8G18_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HEE9797537.1"
                     /translation="EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            242..475
                     /locus_tag="R8G18_003953"
     CDS             242..475
                     /locus_tag="R8G18_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEE9797538.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(508..1146)
                     /gene="ompW"
                     /locus_tag="R8G18_003954"
     CDS             complement(508..1146)
                     /gene="ompW"
                     /locus_tag="R8G18_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HEE9797539.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1386..1475
                     /locus_tag="R8G18_003955"
                     /pseudo
     CDS             1386..1475
                     /locus_tag="R8G18_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1503..2246
                     /locus_tag="R8G18_003956"
     CDS             1503..2246
                     /locus_tag="R8G18_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HEE9797540.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2276..2815
                     /locus_tag="R8G18_003957"
     CDS             2276..2815
                     /locus_tag="R8G18_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEE9797541.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            2920..3318
                     /gene="yciA"
                     /locus_tag="R8G18_003958"
     CDS             2920..3318
                     /gene="yciA"
                     /locus_tag="R8G18_003958"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEE9797542.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3358..4077)
                     /gene="tonB"
                     /locus_tag="R8G18_003959"
     CDS             complement(3358..4077)
                     /gene="tonB"
                     /locus_tag="R8G18_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HEE9797543.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4168..5688
                     /locus_tag="R8G18_003960"
     CDS             4168..5688
                     /locus_tag="R8G18_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEE9797544.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(5721..6491)
                     /locus_tag="R8G18_003961"
     CDS             complement(5721..6491)
                     /locus_tag="R8G18_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797545.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSV
                     KNGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
     gene            complement(6665..>7064)
                     /locus_tag="R8G18_003962"
     CDS             complement(6665..>7064)
                     /locus_tag="R8G18_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HEE9797546.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT         1835 a         1613 c         1638 g         1978 t
ORIGIN      
        1 gagccagtta ctgaattgta taccgacgtt gtatgggcgt cgttcaacac actgcacaat
       61 aaaattaatc cgtataagga aaactgatat ggcaactttg acaaaaaaag aacgggcatg
      121 gttgaacgaa ttacaggaag ttcttgatcg ctgtcctgtg gtgtcatcct gccgcgattg
      181 cgttcttagt ccgtggctat tgcaccacca tcacgcgaaa gggacagcta agcgcggcgg
      241 gatggttaag ccagcaacat taaccgttgc atttaaaaaa gcccgggatt ctgtggatta
      301 caactggcgt gctaatggca ccccaccctc tttccatgag cagagatctt tatcagagcg
      361 attgttcaga gagcaggggg ttgataccaa aattttgcta ggccattcga atcaaaaaat
      421 gaccgatatt tacaacgatg tacgcggtaa ggagtggaaa aaactggtca tttgataacc
      481 agttttgcag aggttttgca gagaaaatta aaagcggtac ccggcagaga acataaacac
      541 ccacggatcc agtcgtaccg agtcttttac ggtagtaaca ccagatttgt acttagccgt
      601 ggtatcgata tccatgtacc acactgacat gttaaccaac cagtcacggt taatcagata
      661 atcaaccccc acctgcccgg cagctcccca gtaatctttc agactgagat cggaaagccc
      721 cgcctctttg ccatgatcgt taaatccatt atcaaagaag gtggtgtagt taatacctgc
      781 cccaacataa ggacggaatt tgctgccggc atcaccaaaa taccactgcg ccatcagtgt
      841 tggcggcaga tgatgaacgg ttgcaatatc gccggtcgcc cgggtgccga ttttatggcg
      901 gaacggcgtc gctgccagta attccacacc aatgttgtcg gtcgccatat aagtaaacgt
      961 caggcccagt tgcgtgttat tggtcacgct gaatccaccc agacttccta acgtaccacc
     1021 agcaccttct gttggacgta cggttgcaga acctgcacgc ataaaaaatt cgcctgcttc
     1081 atgcgcaaag gcactgccag agagaagagt tgttactgcc aaagccgcca ctgttaactt
     1141 tttcatatcc gctccgtcgt tatggttata aaaacgtgac gaatatacct acattggagt
     1201 aacaaatgat cctacataga tcacattaca tatgataatt caccctttat tgatctggat
     1261 taatttagaa gttgcattga gaatcctggt taacgtgatt taaatcaatt tttagatatt
     1321 ttcgctacgc aaatattaac tcgttcgcca cttgccgcgg tttgcccacg cctgaaggcc
     1381 agcaattacc tccgctttac aaagatatgc ttttccatac aagacctgat gctgccaccc
     1441 gaggcttaac cagggtacaa ttgcccgcta attaacacct gcataaaact caaggagagt
     1501 gcatgtctat cacggcgcag tccgtatacc gtgacaccgg aaatttcttc cgtaatcaat
     1561 ttatgaccat tctgttggta tcgttgctat gtgcgtttat cacagtggtg ttagggcatg
     1621 ttttctcacc cagtgatgca cagcttgcgc agctcaatga cggcgtgccc gttagcggca
     1681 gtagtgggtt gttcgacctg gttcagaata tgtcaccgga acaacaacaa attttgctgc
     1741 aggcttcagc ggcgtccact ttttcaggat taatcggtaa cgccattctc gccggaggcg
     1801 taatattaat tatccagctg gtgtctgcgg gtcagagagt cagtgcgctg cgagctattg
     1861 gtgccagtgc gccgatattg cccaagttat ttattctgat ttttctgact acccttttag
     1921 tacagattgg catcatgctg gtggtcgttc cgggaattat catggccatt ttactggccc
     1981 tggcaccggt gatgctggta caggacaaaa tgggcatttt tgcctcgatg cgtagcagta
     2041 tgcggctgac ttgggcgaat atgcgtctgg tggcacccgc agtactgagc tggttgctgg
     2101 caaaaacact gttgctgctt tttgcctctt cttttgccgc attaaccccg gaaattggtg
     2161 ccgtactggc gaacaccttg agcaacctga tttcagccat attgctcatc tatctgttcc
     2221 gcctgtatat gttgattcgc caataacctt aagcatttga ttacggaatc gtaaaatgaa
     2281 gcagtttctt gattttttac cgctggttgt ctttttcgcg ttttacaaga tttatgacat
     2341 ctatgcggct actgcggcgc tgatcgtcgc cacggcgatt gtgcttatct atagctgggt
     2401 tcgctttcgt aaggttgaga agatggccct gatcactttt gttctggtgg tcgtcttcgg
     2461 tggcttgacg ctgttcttcc acaatgatga gtttattaaa tggaaggtta cagtcattta
     2521 tgctctgttt gcgggagccc tgttagtcag ccaatgggtg atgaaaaagc cgctaattca
     2581 gcggatgctg ggtaaagaac tcacgctgcc gcaaccggta tggtcgaagc taaatctggc
     2641 ctgggctgtt ttctttatcc tttgcggtct ggcaaacatc tacatcgcat tctggctgcc
     2701 gcaaaatatt tgggtcaact ttaaagtctt tggcctgacc gcccttacct taatcttcac
     2761 attgttaagc ggtatctata tctaccgcca catgccgcag gaagataaat cctaaccaga
     2821 ctgccagtcc ttcggggctg gcgctgtatc tcctctgttc tcctcaggaa aatcatagta
     2881 gcatcgcgcc tgtgattttc cttttaagtc ggttttacca tgtctacaac acataacgtc
     2941 cctcagggcg atcttgtttt acgtacttta gccatgcccg ccgataccaa tgccaatggt
     3001 gacatctttg gtggttggtt aatgtcacaa atggatattg gcggcgctat tctggcaaaa
     3061 gaaattgccc acggtcgcgt agtgactgtg cgggttgaag gaatgacttt cttacggccg
     3121 gttgcggtcg gcgatgtggt gtgctgctat gcacgctgtg tccagaaagg gacgacatcg
     3181 gtcagcatta atattgaagt gtgggtgaaa aaagtagcgt ctgaaccaat cgggcaacgc
     3241 tataaagcga cagaagcatt atttaagtat gtcgcggttg atcctgaagg aaaacctcgc
     3301 gccttacctg ttgagtaata gtcaaaagcc tccggtcgga ggcttttgac tttctactta
     3361 ctgaatttcg gtggtgccgt taattttaaa caggatattc accacaatcc cactgcctgg
     3421 cttacccggc tcataacgcc atctgcgcat cgcatttttc acctcacgct caaacatgtt
     3481 cgcaggcttg gctgagagga tttgtacgtt atccacgcga ccatccggcg tgacgtcaaa
     3541 tttaacttta acctgccctt caatgcgcaa tgcctgtgct cgtgccggat actgcggctg
     3601 attacggctt aatgcgcgtg gtcctgaagc cacactggta accggcttgc tggttgcagc
     3661 cgttgccgta cttgatgtgg ggcgcgcggg tgccgtattt tcaaacggtg atgccggacg
     3721 cgactctacg ggtttgacat cgcgttttgg ctgctcctgt acctttttca ccggcttcgg
     3781 ttttggctta ggtttcggct tcggcttttc aatgaccacc ggtgcttctt ttggcggttc
     3841 ggggatcggc tcaggttccg gttctggctc taccaccggc tccggtggcg gctgaacggc
     3901 ttgtggcggt tcgagatcag caggcgcaac catcgtgaca gaaattggct gcgcaggcgc
     3961 aggtagttca ataacctgat gtaccgaggt atagagcaga cccgccacaa cagcaccatg
     4021 aatgcagacc gaaagtaacg tcggccaggg gaagcggcga ggtaaatcaa gggtcattgt
     4081 attgtagcta ttcggcattg tagcattagc cataacgttt ataagttcat ctttaaatga
     4141 taatcaattg catgggagtt tatcaccgtg agaaccgtaa taacatactt gagattctca
     4201 tcagccatac aaggcgctga aggtgcagat tcaaccagac gacaaaacga cctgttcaag
     4261 cagtggctga agaagaatgg cgatgctcaa atagtagcgt ctttcagtga tgaagggttg
     4321 agtggttaca aaggtaaaca ccttactggt cagtttggtg acatgttagc ccgtattgag
     4381 gctggggagt ttccagaagg tacaattctg ctagttgaga gtatcgaccg tataggccga
     4441 ttagagcatc ttgaaacaga agccttgatg aaccgcatct taggcaacgg gatagagatt
     4501 cacacattgc aagatggcct tatctacaca aaggatgctt tagccgatga ccttggcata
     4561 agcatcatcc agcgtgtcaa agcctacata gcgcatcaga aatctaagca gaagtctttc
     4621 cgtgtttctc agaaatgggg gcaacgtgca aaacttgccc ttgctggtga acaacgtctt
     4681 acaaagatgg ttcctggatg gattgaccct gagactttta aactcaatga acacgctgag
     4741 actgtgagat tgatattcaa actgctgcta gatggtgaaa gtctgcataa cattgcacgt
     4801 caccttcaaa gcaacggtat aaagtcgttt agtcgccgta aagatgctaa tgggttctct
     4861 gttcactctg tacgcaccat tctaaggtca gagacaacaa taggcacgtt accagcatca
     4921 caacgtaatg accgccccgc tataccgaac tactacgaag gtgttgtaga tataccaacg
     4981 ttcaataaag ctcaagagat tctcgacaag aatcgtaaag gccgtacacc tgcaagtgac
     5041 aacccactaa cgattaacat cttcaaaggt ctgtttaggt gtcagtgtgg ggctagtgtc
     5101 catcctaccg gaacaaagaa taagtatgct ggggtctaca ggtgcaataa ccacttagac
     5161 ggtcgctgtg atgttccacc gttgaagcgt aaaccgtttg accgatggat gattgataat
     5221 tttctgggga tgattgacgt ggggaatgat ggagaatcag agagaaagat tgcagcgtta
     5281 cagcatgagg ttgaaattgt cacagccaga atcaagaaag ctaccgccct acttcttgag
     5341 atggatgata ttgatgaact aaaaattcag cttaaggaac tgaaccagaa gcgcacagaa
     5401 ctacagacca cgattgataa catgaggcgt aaggcttcac tcactgataa ggaactaccc
     5461 cagcttaaag atattgacct tatgactaaa gcgggtcgtg tcgagtgtca gttgattttg
     5521 tccaagcatc taaaagggct tacattgggt aaggattcgg taactgtaac gctacagaac
     5581 gacactgaaa tcaccatacc tacaaacccg ttaccgctaa atgatgggtc accaatcttt
     5641 gaaattgctg ataaagagct actggatatt gacgcttatc aactgtaatt ttcaggggct
     5701 atgaagcccc tttttatgtc tcaataagtg cccttcttgt tagggttcac agagtacttt
     5761 cgcagagcat tacccggctt aagcaccttt gaagcttgct cattagcggc atctgagaag
     5821 gtgctctcac catttttaac gctaacgctc ttagagcacg ttacacgggc ttttagcgct
     5881 tcatctgata actcacttgc cgaatagcca aaaagctctt tacaggtcag catagggctt
     5941 ccaaggctcg ttgtagtgtt tccggtgttc tttttactgg tagcaccgcc atcattcgtg
     6001 gtagcggtct ttccagtagc agccgaaaca tcagtctgac ctacgattga acgaaactct
     6061 acaccttgga aggtcatctg aatacgaaga ccgttagcaa tggtatgctc tttactcgct
     6121 ttaaactgag taatgatact atcagtgata gctattccat ctctacaaac aatagtgatg
     6181 atttgcttct ggtctcgcca ttgttcaacc gtagaaataa aggtttctac aagttgcccc
     6241 tgtctggtta atagaactga acccgtcgaa tcaaccacta caacaccgct aatagagatg
     6301 gtcttaggtg ctctctgaac attatcagtg atagtttgcc ccgattgcat agcctgagtt
     6361 gtcaccatca tcggtgagtc atagtccatc tgttcagtag ctgttaaggc aagaaaagat
     6421 tccacattat ctctaaggtg aaaatagata ccatctttac ctgagtatct aatttgcttt
     6481 gtgtcaaaca ttgttgttat tcctattctg ttaagcgtga gctacgtgaa atctaatttt
     6541 tgatgttggt atctgttcag aagatttcac gtagcattcg actattcata gttgaatcgt
     6601 catactagat aaagattaat agattgccat gtgtggcaat aaatattagt agctctaaca
     6661 ttgtttattc ctctggataa gctgagctaa tcaaggtatc ttcctgcata cgattaatat
     6721 gcgatgtggt tctttcatca ataacctttc catcaagtac cgtctgagtt gtgatattga
     6781 ttgtctgagg ctgaatattt actgacggtt tgataacctt taaatcttgt gtgaatgttt
     6841 ccactcgacc tttcagatta tttaatgctt caggtgaaaa ctgtggaaca tcttttgatt
     6901 tgccctgaac catagcccat aaaacagcgg ctaaagagtt catgtcacca gtcaatgctt
     6961 ggtctgtggg ttccaccttt gaccttacaa ctttatcaat tggattatct gacttactca
     7021 tctgacgttc atagaacggt ttgacaatgg cattaatcac atca
//
LOCUS       DAQSWQ010000165         6825 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.167, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000165 DAQSWQ010000000
VERSION     DAQSWQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.167"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(155..667)
                     /locus_tag="R8G18_003963"
     CDS             complement(155..667)
                     /locus_tag="R8G18_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEE9797547.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="R8G18_003964"
     CDS             862..1626
                     /locus_tag="R8G18_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797548.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="R8G18_003965"
     CDS             1577..2980
                     /locus_tag="R8G18_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEE9797549.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="R8G18_003966"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="R8G18_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="R8G18_003967"
     CDS             4337..5080
                     /locus_tag="R8G18_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEE9797550.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="R8G18_003968"
     CDS             5084..6304
                     /locus_tag="R8G18_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HEE9797551.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="R8G18_003969"
     CDS             6308..6814
                     /locus_tag="R8G18_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797552.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1815 a         1622 c         1868 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacga
//
LOCUS       DAQSWQ010000166         6696 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.168, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000166 DAQSWQ010000000
VERSION     DAQSWQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.168"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..737)
                     /locus_tag="R8G18_003970"
     CDS             complement(<1..737)
                     /locus_tag="R8G18_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HEE9797553.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="R8G18_003971"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="R8G18_003971"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEE9797554.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="R8G18_003972"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="R8G18_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEE9797555.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="R8G18_003973"
     CDS             4397..4678
                     /locus_tag="R8G18_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HEE9797556.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4970..5512
                     /locus_tag="R8G18_003974"
     CDS             4970..5512
                     /locus_tag="R8G18_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9797557.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5592..6266
                     /locus_tag="R8G18_003975"
     CDS             5592..6266
                     /locus_tag="R8G18_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEE9797558.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6282..>6696
                     /locus_tag="R8G18_003976"
     CDS             6282..>6696
                     /locus_tag="R8G18_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEE9797559.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1771 a         1513 c         1601 g         1811 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg attttaattg catttagaaa gccgaaatca tttatataaa tgatttcggc
     4741 ttttttattg atatcaacaa taccatttac ataaatctac attaattgat agatgcgata
     4801 aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat ttgtttcaaa
     4861 taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat ttgtttccaa
     4921 gagatacaga ctcttaacaa ccaaaaaaaa ttttattaag gaacatatta tgaaacgttc
     4981 aattattgct gccgctgtct tttcttcttt ttttatgagc gctggagtat ttgctgcaga
     5041 cgttgatacc ggaacattaa ctattaaggg gaatattgca gaatctccgt gtaaattcga
     5101 agcgggtggt gattcagtaa gtattaatat gccgactgta ccaaccactg tctttgaagg
     5161 taaagctaaa tattctacct atgatgatgc agtcggtgta accagcagca tgttaaaaat
     5221 tagctgcccg aaagaagttg ctggtgtaaa actctcgttg attaccaacg ataaaataac
     5281 cggtaacgat aaggcgatag ccagtagcaa cgataccgtg ggttactatc tctatttagg
     5341 tgataacagc gatgtcctgg atgtttctgc accttttaac attgagagtt ataaaacagc
     5401 ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag ataactcagt
     5461 gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt aataaataaa
     5521 attgtaagtt tctccaggta ataaacatgg ccgctatccc atggcggcct tttaatttaa
     5581 gaggcattaa aatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac
     5641 atgccggaat tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtga
     5701 tggtgcagtt gatgaaccag ggaaaccgtt cttcgctgct gcaggcgtgg attgatgatg
     5761 gcgatacgtc attaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg
     5821 caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc
     5881 ccactaataa agaaagcatt ttttatctga atgttctgga tattccacca aatagtccag
     5941 agcaagaagg taagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc
     6001 gaccagcggg tattgctccg gtaaataaag cgacatttaa aaaattgctg gtaaatcgca
     6061 gtggcaatgg tttggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca
     6121 aagctaataa tgtcaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga
     6181 gtgttaatgt caaaagtaat aatgcaaata actggcatct gaccattatc gatgaccatg
     6241 gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac
     6301 ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct
     6361 ttgataccca ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg
     6421 atgataatca acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc
     6481 gcgggaaata tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag
     6541 ttatcaagcg gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat
     6601 ttgagcaact tgttcagggt gggagctata cctgggatat cggggttttt cgtctcgatt
     6661 tcagtgtccc gcaggcctgg gtggaagaac tggaaa
//
LOCUS       DAQSWQ010000167         6567 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.169, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000167 DAQSWQ010000000
VERSION     DAQSWQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.169"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..548)
                     /locus_tag="R8G18_003977"
     CDS             complement(<1..548)
                     /locus_tag="R8G18_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797560.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLL"
     gene            complement(595..4452)
                     /gene="sigA"
                     /locus_tag="R8G18_003978"
     CDS             complement(595..4452)
                     /gene="sigA"
                     /locus_tag="R8G18_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HEE9797561.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4814..5818)
                     /locus_tag="R8G18_003979"
                     /pseudo
     CDS             complement(4814..5818)
                     /locus_tag="R8G18_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5803..6180
                     /locus_tag="R8G18_003980"
                     /pseudo
     CDS             5803..6180
                     /locus_tag="R8G18_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6219..>6567)
                     /locus_tag="R8G18_003981"
     CDS             complement(6219..>6567)
                     /locus_tag="R8G18_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEE9797562.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1625 a         1459 c         1484 g         1999 t
ORIGIN      
        1 agaagtactg agcttacttt acctttgatg atgtaatagt tatttccgtc ggtactcatt
       61 tttccctgaa ataatgtcac ggatgtttct tcctgctgat cctgcaggcg cagtcgtaag
      121 gtcatatttt taatgagctg agatccgggt ggcaaggggt atttgctgtc agatactggg
      181 cgactgagtc tgatgtggcg cgtatacagc ccgttttcgt acagggtggc ctctgttatg
      241 gtgtcagtga ccagcatgtg ggagacaatg ctgtattctt ttttaaagca ctcaccagaa
      301 acggtgattg taatgataat ggtcttctcg atcgagatgt aaatacccgt ttcgttatac
      361 tgtccgtatc ggaaccatga gaactcagtt gcattccagc catgctccag aatatgtgca
      421 tttttttgcc tgatagtttc tttttttatt cgctcatgtt taaggcataa caggcagata
      481 aataatgtca gaaaaagaaa gctaataatg agagcaagag cgatatagtc tgtcacaggg
      541 taatacatat tctctacggt agaagaaggg ccgcaaacgc ggcccgggct gttatcagaa
      601 agagtaacgg aagttggcgt tgatcgcgtt atccacgttg tatttaccaa atgccgattt
      661 ctcaaactca agaccgaagc gaagattatc gcgaatttcg gcgttgagac caacattcat
      721 gagaatacga ccgtcttttt cacctttgat acgtttctca ccggacgcat cacgcagtac
      781 ggtttcaccg ttggcaaaca ggtcaaactg gtagccaagg ccggcgcggg ctgtgacttt
      841 ccagtcctta ccggagaagg atttacccac atcaacaccg gtacgcccaa tcagcggatt
      901 aaagtcctta tccttcatgg tcacgctcat tccgcgatcc tgccagtcga accgtttacc
      961 tgatacggcc ccataaacca gttctgcctg cggctcaatc caggagtctt ctgttacatg
     1021 atagcggtag cctgcttcag cacccacata caaggaatga gaactgtagt cttttgttcc
     1081 gagtccagca aaggtcgccg agtactcatt atcatggtga acatacttac taatcaggtc
     1141 gatataggca ccagagtcaa atatagcgga agcatacaga cctgccccga cggacttcgt
     1201 cttgccactg aatgcattac tgctggcatg actgtcggta tacgtcatag tcagaccagt
     1261 gaaaaggtca agtccatcca gctcatgttt tttatccaca ccaatctgca catgggtgta
     1321 gttgtcactg tatccactag aagctgaacc tgctccgctc atgatgcggg cccatgcacc
     1381 ggcctcgcca ttggtgtcac gcagatcccc cattcgttta ttcaggttgt tgacttctgt
     1441 caggaaggct ttgtaattac cggacatata cgattttgct gcaccggaag atgctgtatt
     1501 ttcagccacc ttatatccgg tcagggtcca cgtggtatgt tcctgatcct ctttcgtgct
     1561 gatcacagga gatatgttgc tgaaacctat ggcctgagta ctggctttaa atatctcttt
     1621 attgcttcct tttggggctg ttatcaggtc aacgtttaat cctgagttat tatttcccac
     1681 tttctgtata tcgaccgaca gactgttatt gcttccggtt aactgatttt taacaatgag
     1741 tttatccgat tgatttacag actggctcag atcagcccgc ataatcacag cagtattagt
     1801 ggcatccagt gtgtttactg tcacagtatg gaaacccgaa ttctctatgt tactaaacaa
     1861 caaaagactg tcgcgggttt ttaatgattg cagggctgac tgtccgttta ctctccataa
     1921 ggtgttatac atgctggctg aggcagcagg agcatcaata cctccggtat aagaagtatc
     1981 aaagccaccc agcaaacctg tagccagcgg tgtgtgtgtg gttttgtcca ggttatcttc
     2041 tttatactgg tcaacaccaa acagaataga tgcttttttg tctgatttta tattaccatt
     2101 gacccaggca taattttgag cctttaacgt ggcaccatct tctgtcatat tatatccatc
     2161 tgacatatag attgccgggc tgtatttatt attttctgta ggggtgcctg ttaatgtcag
     2221 ggttccggcc agttcaatag ctttatctac atacattttt ccctgggcgg taacagtagc
     2281 tcctttgttg ataatcaggt ctgtattaat taaggataat gcaccagtca tagtaacagt
     2341 tgatttatca ccaacagtta ttttgctgtt ttgagcactc agactgccgt tgaaactact
     2401 ttcgcctgct gaatccccgg agcgaagctg ttgtctgaat ccaaaacctt cgccagtaat
     2461 gtttcttcct gagaacatgt caatgtatgc agttttattt cccagaacaa tttgagagtt
     2521 tttagcctct atgtgtcctt ttacattagc atcccggcct atactcaggg ttgcgttttc
     2581 taaatttaat ttccggaaat taaatttacg actttcccag tcgggctggg aaaaagaggc
     2641 tatctgattg ttggacttat attccgtatt gtaacgttta tttacagtgc tttcctggtc
     2701 ctttatggca gcagaatagt ctttttgaca taaaaaagga attggacagt tgttattgcc
     2761 ttctttaaat atggcatgtt ctgtcgcatg tccttgtaat gttaactgtg cattccggac
     2821 agagatatcg tttttgatat cgactgagcc atcaaagatc agattggcat tattgttatg
     2881 ttgctgggta gtattgataa tatgatttat gcttatatta cctgatacat tcccatggta
     2941 catatatgca tcagtattag taagtgataa tgttgattgc ttcacgtttg agttcgttac
     3001 ggttgttccc gcatctgttg ctgcgatttt ctgaaatgac tggtcatagc cgtttagatc
     3061 cagtgttccg ccatttctgg taaaatagat ttcgccatta ccgctttcgc ttaaagcgtc
     3121 tttggcattt atttttaccg tacctttacc tccggccatg taaattttat tgaaggcttt
     3181 ttcagcacta aggatgacag tcccattacc tattttaagg ttatttccct gtgctatttt
     3241 tacatccaga gtaccactac ctattttatg cagattatct ccggcaacgc ccttaacatt
     3301 ccattcaacg gtggtattat tatcaatatc tatcccggca cctttataag acttatcttt
     3361 tccgtacact ttatatgtct ggttcttatc gaatataagc ccgccaatgc caagatcggt
     3421 atcctgcttg agcgttaatt cccctcctcc tgagacagac aggtccttat cttttataat
     3481 cgatgataac tcttgttttt taccatccag ctgcatagtg tttgtgaaat tcttttttaa
     3541 attgtttacc agaatattat taaataacga gtagtttgtc caggctatat tggtactact
     3601 gagaaaacca actgtactgg taacaccatg gaaaacccat ttttgttgtt ctttatcata
     3661 tagataagaa ccagaaccac tatcgccaaa aattggtgag tttttaaaag gggttctgtt
     3721 ggtataaatg taactcttgt ttttccagtc cagattaaaa agacttgcgc ttaacaagtc
     3781 gggattatag ttttgtccaa acagatattg tttgccgttt aatatcgttg aggttgttcc
     3841 ggcacctgct ctgaagccaa ttatttgttc cttaccgttg tagtttacgc catatctttt
     3901 catcgcatct tcgggagaaa gtgagaagtc ggcactctcc gtaacccctg ttgtttccac
     3961 gacgaattta ttcagacgat gaaccgaaaa gtcgccagat gatactctgt tacttgcttt
     4021 atatgctgtc tgatcccatg actgtgttgt tattgcatga tgctgtgtac cgttatgagt
     4081 cgcagtaaca ctgtacgcac ctccaattga tgttgttgca cctttgttgg aaacagcaga
     4141 aaaatcaggt acaggtagtt ctggaaaatg aaatatcttt ccatctttca tcattaattg
     4201 aacatttgtt gcacccggtc tgaactctcc tctgttttga gcaaggtcaa gataatcgcg
     4261 ggtccatata ttatttatat ccagcattgc tgcaaatgct gtttcgccat aacttccata
     4321 tattgaggat aaaataatac cgaggataac tttctttctt gatgtaccga cactaacttt
     4381 ccgggtcagt tcagaaacag caactaatcc acctgtaata tgactatatt tcagtgaata
     4441 aattttattc atacgtgaac tccgatattt ctgagttgtg gttatgggat agcatttaat
     4501 tacaacgggg aactatcagt catggtacgt aactgataag ggtataatga taatcactgt
     4561 cagttatatt tgtatgacag atgtaacaaa atgaaaaaaa aagataagca caagtgattg
     4621 attggtcaga tattgatcac ttggtgtttg tattttgaac tttgcgatca aaatttgatc
     4681 tttgttgttt ccggtcaatc ctttacaggt tttctgtatt atccggggga gttaagtctg
     4741 tcgatgtccg acagactttt gatgatggaa acaacatgca gcgataatgt gcatgctgtt
     4801 ttgcaggtgg aactcagaag gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg
     4861 tgccgatcag gcctgcaaca tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg
     4921 ccgtgaatgc actgccgtcg ttaccggcca gattctgacg gtaaccggcg cgcagctgtg
     4981 cccatttcca ggcattaaat tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg
     5041 tgaaaccgct ggccggggtc agatccacat caaatgcggt ggtgaacatc gcgttgtgcc
     5101 aggagacacc ggccgtcacc tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt
     5161 cttttgtctc aatactacgg gggatcaggt tctgtgcgac cagtcccagc gtccagttgt
     5221 catccaggtc cgtactggca ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc
     5281 ggtcgccttt gaaggcgctg ctgtcataat ttctgaccag cacgttgtag ttgaacaggt
     5341 cgacgcgctg gaatttgggg gggaagccca gagaccattt ctgcccggcc gtttccagtt
     5401 ccgtagccag agcaataccc acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg
     5461 tcaggcggct tttgtccacc tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga
     5521 ggtcagcatc agagacttta ccgtcgacac tgacggtgcc gtgtgattta accatcagtg
     5581 cggcagagag gcgatcaccg ggaagggccg ccacagcaga aacgcccagc tgtgcggcgg
     5641 catgtgtgtt acggaagtcc cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac
     5701 cgtggttact ttcgatggcg cggtcgaagg ccttccactc atcactgatc cggtcggcgt
     5761 tgtcggtgat attatccgga ttggagagct gcgcgccgac ggacggaagc aggatgctgg
     5821 gctcatgcgc gacgcaaaat ccacgatgta tatatcagta ctaaaagcgc gacggcggaa
     5881 gaagctctga aactaatcgg tgaactgtac gccatagagc acgatatacg cgggttaccg
     5941 gtgtctgaac gcctggcggt caggcaaatg cagagtagac cgctcctgac ttccctgtat
     6001 aagctgatgc aggagaaaga acacacgtta tcgaagaaat gtcgtctggg agatgcgttc
     6061 cggtatatca ggaagcactg ggctgcgttg tgcaacttct gtgatgatgg cctggctgag
     6121 gtagataata atgcagcaga aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt
     6181 tcgaggatca ctcattccgt gtctgactgc ttcagggttt aattttgctt ctgctgactg
     6241 aatgatgatt gggcagcaag tgcaatatcc cacataaatc cagttaactc acgagccacc
     6301 gccgtaatgg ctacttgtga ttttttccct tttgctgtca gagttcggta acgagaacac
     6361 aatcgttgtt gagctttcca tgcaatatct ttggaatatt gtgttacaga gtccggtcgg
     6421 taatatataa gccatgctcc aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa
     6481 agcagacgtc tcagttcgct gtttccaact tttgttattc ctctgggacg aatactgttt
     6541 ccactggaat attcacctgg caccagt
//
LOCUS       DAQSWQ010000168         6553 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.170, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000168 DAQSWQ010000000
VERSION     DAQSWQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.170"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="R8G18_003982"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="R8G18_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HEE9797563.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="R8G18_003983"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="R8G18_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HEE9797564.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="R8G18_003984"
     CDS             2408..3157
                     /locus_tag="R8G18_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEE9797565.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3745
                     /gene="yieF"
                     /locus_tag="R8G18_003985"
                     /gene_synonym="chrR"
     CDS             3179..3745
                     /gene="yieF"
                     /locus_tag="R8G18_003985"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HEE9797566.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="R8G18_003986"
     CDS             complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="R8G18_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HEE9797567.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5302..5967
                     /gene="yieH"
                     /locus_tag="R8G18_003987"
     CDS             5302..5967
                     /gene="yieH"
                     /locus_tag="R8G18_003987"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HEE9797568.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6034..6507
                     /locus_tag="R8G18_003988"
     CDS             6034..6507
                     /locus_tag="R8G18_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HEE9797569.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1609 a         1655 c         1658 g         1631 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggctgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagtatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     3481 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     3541 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     3601 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc ggcaaaccgg
     3661 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     3721 gtttattcag cgagttaaga tctaaataaa aaacccgcca gtaatcatgc atggcgggtt
     3781 tttaatgcgc tatcgatttt aatgagcgtc gataaataca atcttcagga taaacagcag
     3841 cgcaacgatg attacgcacg ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac
     3901 gcagtaggag ataaagccca gcgcaatacc ttcggtaatc gaaaagctga acggcatcat
     3961 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     4021 acttgaggtc atcaacacgc caacgtagat caacgcgcca gctgcagcgt agcctggcac
     4081 cattcccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     4141 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     4201 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     4261 cttcatgcgc gggaatttcc ccttctcatc cgccagacct gctttatcgg tcacgccaat
     4321 cagcgtaccg gaggagtcaa acaagttgac caacatgaaa gagaaaatca cccctgccag
     4381 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     4441 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     4501 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatgg cgatgatgaa
     4561 gaaaccgagg atccccagca gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     4621 ttccgggtta gcgacaatca cacctgcgtt tttcagcccc atcatgccaa tgaacagacc
     4681 gataccgctg gtaataccca cacgcagact taccggaatg ttagcaatca tccagtaacg
     4741 aacgcggaaa atggtcaaaa gaagcagtcc gacagcgccc cagaagattg cgcccatacc
     4801 gatctgccac ggcagcccca ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc
     4861 catcgcagga gccagcgcca ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa
     4921 agcagcaatc agacaggttg ttacgaagac ggcactggtg tccatgccag caacgcccag
     4981 aatttgtggg ttaacaaata cgatgtagac catcgtcagg aaggtggtga acccggcaat
     5041 cacttcagtt cgtgccgtag taccatgttc gcgcagttta aaaactcgtt ccagcatccc
     5101 ctgaccagaa gtctgggtgg tatgttgatg actcattatc tatttccgaa caaggaggga
     5161 aaatccgtcg ctatcgtata ccaaaatgcg acaataggcg cgtttgtgag agactttttt
     5221 tattggattt acttatacgg caacgattgc gttgcgcaaa tcggctctac gaaaacgtta
     5281 aactgattaa aaaggaaagg catgtcccgg atagaagcgg tatttttcga ctgcgacggt
     5341 acgctggtcg acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt
     5401 ggtattacgc tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa
     5461 attatcgata ttgtttccct tgaacatggt gttacgttag cgaaaacaga atctgaacac
     5521 gtttaccgtg cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct
     5581 gaagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac
     5641 aaaatgcagc attctatggg caagctgaat atgttgcact acttcccgga taaactgttc
     5701 agcggctacg atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa
     5761 gcgatgaatg taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa
     5821 tctggtatcg acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg
     5881 atcgttcacc cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa
     5941 gcgcgtggtt gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa
     6001 ttggcgctca aaggtaagta aagggagttt gatatgtctg tttcacgtcg ggtaatacat
     6061 cacggacttt attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac
     6121 atattcttcg caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt
     6181 ctcttattgt cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc
     6241 tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt
     6301 ggcgtcgtta tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc
     6361 tcggagttgt ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca
     6421 ttgctggcag gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca
     6481 tgtcctgaaa cagactcttt aagttaagcg ggatacttta tctttgggct actcaaaagc
     6541 agacaggatg tta
//
LOCUS       DAQSWQ010000169         6499 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.171, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000169 DAQSWQ010000000
VERSION     DAQSWQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.171"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="R8G18_003989"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="R8G18_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="R8G18_003990"
     CDS             complement(1751..2509)
                     /locus_tag="R8G18_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HEE9797570.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="R8G18_003991"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="R8G18_003991"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HEE9797571.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="R8G18_003992"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="R8G18_003992"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HEE9797572.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="R8G18_003993"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="R8G18_003993"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEE9797573.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="R8G18_003994"
     CDS             complement(5474..6079)
                     /locus_tag="R8G18_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797574.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6499)
                     /locus_tag="R8G18_003995"
     CDS             complement(6082..>6499)
                     /locus_tag="R8G18_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797575.1"
                     /translation="QRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINSV
                     PGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENP
                     LKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1638 a         1695 c         1663 g         1503 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgt
//
LOCUS       DAQSWQ010000170         6371 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.172, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000170 DAQSWQ010000000
VERSION     DAQSWQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.172"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..314
                     /locus_tag="R8G18_003996"
     CDS             <1..314
                     /locus_tag="R8G18_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797576.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="R8G18_003997"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="R8G18_003997"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEE9797577.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2201..2917
                     /locus_tag="R8G18_003998"
     CDS             2201..2917
                     /locus_tag="R8G18_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEE9797578.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3373..3445)
                     /locus_tag="R8G18_003999"
     tRNA            complement(3373..3445)
                     /locus_tag="R8G18_003999"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3410..3412),aa:Asn,seq:gtt)
     gene            complement(3546..5189)
                     /gene="yeeO"
                     /locus_tag="R8G18_004000"
     CDS             complement(3546..5189)
                     /gene="yeeO"
                     /locus_tag="R8G18_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HEE9797579.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5194..5269
                     /locus_tag="R8G18_004001"
     tRNA            5194..5269
                     /locus_tag="R8G18_004001"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5227..5229,aa:Asn,seq:gtt)
     gene            complement(5307..6257)
                     /gene="cbl"
                     /locus_tag="R8G18_004002"
     CDS             complement(5307..6257)
                     /gene="cbl"
                     /locus_tag="R8G18_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HEE9797580.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1812 a         1539 c         1368 g         1652 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgagttt
     1981 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa acacaattat
     2041 aaaaaatccc gccaacaata taagttttta taaaattatg gctttagaat atttttattt
     2101 ctaatagacg agatttttcc tgttatgata taatatgccg aattaacaca tgttaacgat
     2161 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
     2221 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
     2281 tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt
     2341 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
     2401 aattgataaa gccaaaggcg gcggagatga aacgttcgtg cagggacgtt atgaaggctt
     2461 tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtta accgtacgat
     2521 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
     2581 cagctatatg tttgacaatg ctggtgtgat tgtatttaaa ggatcagacc ctgaccatat
     2641 ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa
     2701 cattgttatt tataccgaac ctactgacct tcataaagga atcgcggctc taaaagcagc
     2761 tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct
     2821 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga
     2881 tgtacaaaaa gtttatcata acgtcgcaaa tctctaatta tcttttaaag aaatctgtct
     2941 ttacggcaga tttctttaat ctcatataat tcttataaaa aatataagat tcaactcgtc
     3001 atattgatta tacccccccg ttcccagaga aataatattt attaaaattc cagttcttct
     3061 ttttctgatt acagaaggca aagtggcaat tacgcatagt ttcccgataa agacgcgata
     3121 gcgacatccc gcataaggca tttttctctt tatctttgta cggtacttca tggaacagag
     3181 tttttgacct tgcgaatcgt gatgtctgtt ggggaggggc aatttgctca ctgaagcgtg
     3241 agactcgatt aagcgcacga aacacagaaa tcaaaaaacc cggtcacttt tttacaaggt
     3301 aaccgggtaa aaataatttt tattttttaa ctgttttgag actcatagag atgtctcaaa
     3361 actaaaattt tgctcctctg actggactcg aaccagtgac atacggatta acagtccgcc
     3421 gttctaccga ctgaactaca gaggaatcgt gagaacgagg cgaatattag cgatgcccac
     3481 ccacaatgtc aaagcctgtt ttttaaattt gaaatcgttt gctgaaataa tctgcatttt
     3541 gtcgtttatt ccgacacaac tggctttttt tcacactttt gcggttcgga tcgagggtat
     3601 ttccacagcc aacgtccagt aaccattcgc cagtaaaaca gcacggcccg cacagcccag
     3661 tcggcaaaca ttcccatcca gacaccaacc acaccccagc caagcatgat gcccagcaca
     3721 taaccgacga caacccggca accccacatg ctcaacatag aaacccacat ggcgtaacgg
     3781 gcatcgcgag cacctttaaa tccagcgggt agcacccatg aggcggacca aataggcata
     3841 aataaagcat ttagccaaat cagaatcaca acgacatgtt taacctgtgg atcctgggtg
     3901 taaaacgatg ccataacccc ggcaaaggga gccgttagcc aggcgatggc cgttaatcca
     3961 agagtggaaa gccagaacac atgccgcaac tgaatctctg cttgcgctat ctgccctacc
     4021 cccaaccttc ggcctgtaat gatcgtagaa gcagagccga gcgcacttcc gggtaagttg
     4081 ataagagccg caattgaaaa cgcgataaaa tttccggcaa taacactggt ccccatcccg
     4141 gcaacgaaca tttgggttaa taaccgacca ctggtaaata acactgattc gacactcgcg
     4201 ggaataccaa tccccatgac ttcccagata atgctaaaat tcagcggttt aaaatagctc
     4261 tttaacgaaa tccttagcgc aggattaaaa ccaatcgcca gcacccacaa aattgcaact
     4321 gcgccaatat aacgagaaat ggttaaaccc agccctgccc cgacaaatcc cagtcccggc
     4381 caggagaaaa ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta
     4441 ccgttaatca atagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaata
     4501 agagtgatgg cagctgctgg ataactgaga accgtcagct ccagataagt caacgccagt
     4561 gctttaactt ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga
     4621 tgaataagcg ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc
     4681 gccaccctcg ctcgtcgtcg atcccgctta ccgagactaa atgccacaac gacagtagta
     4741 ccaagatcga tagcagcaaa aaaagccata atgaccatat tgaagctgtc cgccaatccc
     4801 acgccggcca tcgcatcttt tcccagccag ctgaccagaa aagtgctcag aacccccatc
     4861 aacaggacac aggcattctc catgaagata ggaacagcaa gcggggttat ctcgcgccag
     4921 aacaacactt tgtagctctt gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt
     4981 aaagcagagg agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat
     5041 aatgagggag aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa
     5101 caaactgttg atagaaacgg caaacagttg gggaatttaa aaatcgggtt tgacaaaaga
     5161 tttttcgccg ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg
     5221 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa
     5281 agcccgcttt tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt
     5341 tccatcacct gccgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag
     5401 cgccagacat agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca
     5461 tcgaaaagat gccgcgtatc cagacggatt aaattctctt cctcttgttc gccgctggat
     5521 tgttcggcaa ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa
     5581 tcctgcgcac ttaatacaat atctgccagc aaacctttgc gggcaaatgc gtcatcaata
     5641 cgtgagcgcc ccgtaatccc ctgtcggtaa gtgattaacg gccacttcgc tattgattcc
     5701 agcgtcaatg gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa actatggtgc
     5761 caacgaaacc acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata
     5821 ccaatatcag cttcgccatt ttgcaacaac gtcgcaattt cctgtggcgt cccctggatt
     5881 agctcgagcc gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta
     5941 taacgtgcct gagtatgcgt cgttgcaata gtgagaacgc cagacgtatc gttggtaaac
     6001 aggtctgcaa gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt
     6061 aatgctttgc ccggttcagt catgcccagc agtcgcttac ctcgtcgaac aaatatttcg
     6121 atgccaagtt catcctccag ttcccgaata tgacggctga cgccggactg tgaggtaaaa
     6181 agcatattcg caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt
     6241 agttgttgga aattcacggt aaactccggg cagttcagat ttcccgttat tgttaaagtc
     6301 taatgcccgg cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt
     6361 agctcaccaa t
//
LOCUS       DAQSWQ010000171         6316 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.173, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000171 DAQSWQ010000000
VERSION     DAQSWQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.173"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..411
                     /locus_tag="R8G18_004003"
     CDS             <1..411
                     /locus_tag="R8G18_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEE9797581.1"
                     /translation="VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLA
                     YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD
                     WYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            457..807
                     /gene="traA"
                     /locus_tag="R8G18_004004"
                     /pseudo
     CDS             457..807
                     /gene="traA"
                     /locus_tag="R8G18_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            822..1133
                     /gene="traL"
                     /locus_tag="R8G18_004005"
     CDS             822..1133
                     /gene="traL"
                     /locus_tag="R8G18_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HEE9797582.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            1155..1337
                     /locus_tag="R8G18_004006"
                     /pseudo
     CDS             1155..1337
                     /locus_tag="R8G18_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1339..2589
                     /locus_tag="R8G18_004007"
                     /pseudo
     CDS             1339..2589
                     /locus_tag="R8G18_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2586..2972
                     /gene="trbI"
                     /locus_tag="R8G18_004008"
     CDS             2586..2972
                     /gene="trbI"
                     /locus_tag="R8G18_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HEE9797583.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2969..3280
                     /locus_tag="R8G18_004009"
                     /pseudo
     CDS             2969..3280
                     /locus_tag="R8G18_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            3275..4273
                     /locus_tag="R8G18_004010"
                     /pseudo
     CDS             3275..4273
                     /locus_tag="R8G18_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            4293..5040
                     /gene="traX"
                     /locus_tag="R8G18_004011"
                     /pseudo
     CDS             4293..5040
                     /gene="traX"
                     /locus_tag="R8G18_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            5095..5655
                     /locus_tag="R8G18_004012"
     CDS             5095..5655
                     /locus_tag="R8G18_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HEE9797584.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5785..5997
                     /locus_tag="R8G18_004013"
     CDS             5785..5997
                     /locus_tag="R8G18_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HEE9797585.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1501 a         1530 c         1927 g         1358 t
ORIGIN      
        1 gtggggtggc gggtctcatc gtccatggag acgacattcg tgctggatgc actggagcag
       61 gcgttatggg cccgtcgacc gtccggcacg gtccatcaca gtgataaagg ttctcagtat
      121 gtatcgctgg cctacacaca gcggcttaag gaagccggat tactggcatc aacaggaagt
      181 acaggcgact cgtatgacaa cgcgatggcg gagagcatca atggtcttta caaagcggag
      241 gtaatacacc gtaagagctg gaaaaaccgt gcagaagtgg aactggccac actcacgtgg
      301 gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc tccggcagaa
      361 gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga
      421 taaaacactc tccaggaaac ccggggcggt tcagcgttaa gtgttcaggg tacttctgcg
      481 cccgtcaaaa agaaatcgtt tttttctaaa ttcactcgtc tgaatatgct tcgcctggtt
      541 cgtgcagtga tcccggttgc tgttctgatg atgttgttcc ctgagttagc tatggctgct
      601 ggtaaaggca cggacctgat ggctaaaggt caggaaacgg ttaaggcgac cttcggtaag
      661 gactccagta tcgttaaatg ggttgttctg gctgaagttc tggtcggtgc agtcatgtac
      721 atgatgacca aaaacgtcaa gttcctggtc ggctttgcca tactctctgt atttattgca
      781 gtaggtatgt ctgttgccgg attctgacag gaaataaaac gatgttggga gacgagaata
      841 aacttaagaa atatcgtttc ccggaaacac tgaccaacca gagccgctgg tttggcctgc
      901 cactggatga actgatcccc gcagcaatct gtgttggctg gggtatcaca acatcgaaat
      961 atctgttcgg tattggtgca gcggttctgg tttatttcgg gattaaaaaa ctgaaaaaag
     1021 ggcggggcag ttcctggtta cgtgacctga tttactggta tatgccaaca gccctgctgc
     1081 gcggtatttt tcataatgtt cccgattcgt gtttccggca gtggattaaa tagaactgat
     1141 accaggattg ttatatggaa cacggtgccc gtttaagtac cagtcgtgta atggccatcg
     1201 cctttatatt tatgtcagtg cttattgttc tcagcctctc tgttaacctc attcaggggg
     1261 tgaataacta ccgtcttcag aatgagcaac gcactgccgt gaccccaatg ggatttaatg
     1321 tgccctttgc cgtgtcacac ctaccgtaac cagctggcgt ttatcgatat tttcttccgg
     1381 ggaatgaata acaccaacta caacatggcg gtctgtggca cctccggggc cggtaaaacc
     1441 gggctgatac agccacttat ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc
     1501 gacatggggg atggctacaa gtccctgtgt gagaacatgg ggggcgtata tctggacggt
     1561 gaaaccctgc gttttaaccc gtttgcgaac atcaccgata tcgaccagtc agcggagcgt
     1621 gtccgtgacc agttgtcagt gatggccagc cccaacggta acctggatga agtgcatgaa
     1681 ggtctgctgc tgcaggcggt cagggcctcc tggctggcca aagagaacag ggcacgtatt
     1741 gatgacgtgg tggatttcct gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg
     1801 actatccgca gccgtctgga cgaaatgatt gtgctgcttg accagtacac tgccaacggc
     1861 acttacggcc agtattttaa ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg
     1921 gtgctggagc tgggcggact ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc
     1981 ctgattatct acatcgagaa caggatgtac cgcacgccgc gtaacctcaa gaaactgaac
     2041 gtcattgatg aaggctggcg tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt
     2101 gagaaaggtt accgtaccgc ccgtcgtcat accggtgctt atatcaccat cacacagaac
     2161 atcgtcgact ttgactctga caaggcctcc agtgcggccc gtgcggcatg gggtaactcc
     2221 tcctacaaaa ttatcctcaa acagagtgcg aaggagttcg caaaatacaa ccagctgtat
     2281 ccggaccagt tccagccact gcagcgcgac atgattggta agtttggtgc ggccaaagac
     2341 cagtggttca gttccttcct gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt
     2401 gtggacccgt taagccgcgc catgtacagc tctgacggcc cggattttga gtttgtgcag
     2461 cagaaacgta aagaggggct gagtattcat gaggcggtgt ggcagctggc gtggaagaag
     2521 tcagggccgg agatggcttc gctggaagcc tggctggaag aacatgagaa atacaggagt
     2581 gttgcatgac cacaacgcaa aaaacgactg atgttacagc cccccgacgc agccactggt
     2641 ggtggacggt gccgggatgc ctggcaatgg tgttactgaa cgcagccgtc agttacggca
     2701 ttgtcagact gaacgcaccg atgactgccg ccttcaacat gaaacagact gtggatgcat
     2761 tttttgacag cgccagtcag aaacaactgt cagaagcaca gtcaaaagcc ctttcagcgc
     2821 gttttaacac ggcactggaa gccagcctgc aggcgtggca gcagaaacac cacgcggtca
     2881 ttctggtgtc gcctgccgtg gtacaggggg ctccggatat cacccgggaa atccagcagg
     2941 atattgcccg gcgaatgaga gcggaaccat gagatgccgg gggctgattg ccctgctgat
     3001 atgggggcag agtgtggccg ccgccgatct tggcacctgg ggtgatctgt ggccggtaaa
     3061 ggagccggac atgctgacgg tgatcatgca gcgcctgacg gcactggagc agtccggtga
     3121 gatgggccgg aaaatggatg cctttaagga gcgggtgatc cgcaacagtc tgcggccccc
     3181 tgccgtgccc ggtatcggac gcacggagaa atacagcagc cggttgtttg acccgtccgt
     3241 cagactggct gcggatatcc gggataacga agggcgggtg gccggctttg agtcagccta
     3301 cgtggccctg tcgcgtatga agcagcatgt gcaggtgtac accgataacc gtcagggctg
     3361 gacggatgcc atcaacaatg ccgtacagaa aggaactgcc cacgatgtgt ttgagccgaa
     3421 accggaccgg gaggtcatga atgcagagcg gctgttcagt acggcgcggg agctgcggga
     3481 cgtggcggca ggccgtgccg ttctccgtca ggcggggctg gccgggggag acagtcctgc
     3541 acggtttatt gctccgggac gtaaatatcc gcaaccgtat gtggcactgc cggcgtttga
     3601 ccgtaacggc aagtccgccg gtatctggct gaacccgctg accacggatg acggaaacgg
     3661 gctgcgggga ttcagcggtg aaggtcgggt aaaaggcagc ggggatgcgc agttcgtggc
     3721 cctgcagggc agccgtaacg gagaaagcct gctggctgat aatatgcagg atggcgtccg
     3781 gattgcccgt gataatcctg acagtggtgt ggtggtaagc attgccggtg aaggccgtcc
     3841 gtggaatccc ggtgccatca ccgatggccg cgtgtggggg gatatcccgg acaacagcgt
     3901 ccagccggga gccggaaatg gcgaaccggt cacggcagag gtactggcac agcggcaggc
     3961 tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat gaaattgtcc gtaaaatggc
     4021 agagaacaaa cctgacctgc cggacggcaa aacagagctg gctgtcaggg aaattgccgg
     4081 gcaggagcgt gaccgggctg ccataactga acgggaagcc gcgctgccgg agagtgtgct
     4141 gcgtgaatca caacgggagc gggaagcggt ccgggaggtt gcccgggaaa atctgctgca
     4201 ggagcgtctg cagcagatgg agcgggatat ggttcgtgac ctgcagaaag agaaaacccc
     4261 gggcggagac tgatacagga agacaaacac tgatgacaac ggataacacg aacacgacac
     4321 gtaacgattc actggctgca cggaccgata cctggttgca gtcatttctg gtctggtcac
     4381 ccggacagcg ggatatcatc aaaacggtgg cactggtgct gatggtactg gaccacatca
     4441 atctgatatt ccagctgaag caggaatgga tgtttctggc cgggcgagga gcctttccgc
     4501 tttttgccct ggtgtggggg ctgaatctgt cccgtcatgc gcatatccgg caaccggcca
     4561 ttaaccggct gtggggatgg ggaattattg cccagttcgc atattatctg gcaggctttc
     4621 cctggtatga ggggaatatc ctgtttgcct ttgcagtggc ggcacaggtg ctgacgtggt
     4681 gtgaaacgcg cagtgggtgg cgtacagcag cggccattct tctgatggcc ctgttggggg
     4741 cctttgtccg gcaccagtta cggcattgcc gggctgctga tgctggcagt cagccaccga
     4801 ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgcct gctggctgtt
     4861 attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggtct tgtgatgacg
     4921 gtgctgaccg ttggtctggt gtcgtgtgca ggaaaatcat taccccgttt ctggcccggg
     4981 gattttttcc cggtattcta cgcctgtcat ctggctgtgc tgggcgttct ggcgctgtga
     5041 cgggtgtggt atctttgacc gtaacaggat gattcgtcag aggcagaaca cagcatgaca
     5101 gagcagaagc gaccggtact gacactgaag cggaaaacgg aaggggagac gcctgtccgc
     5161 agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa aaagcagaag
     5221 ctggccgaga aagcagcccg ggaagcagag ctggcggcaa aaaaagcgca ggccagacag
     5281 gcactgtcca tttatctgaa cctgcccacg ctggatgagg ccgtgaatac cctgaaaccc
     5341 tggtggccgg gattatttga cggtgacacc ccccggcttc tggcctgcgg tatccgggac
     5401 gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa actgcgcagg
     5461 gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc cggtgcctgc
     5521 cggtatgaca cggaagggta tgtgacggag catatttctc aggaggagga agcgtatgcg
     5581 gcagcgcgtc tggataaaat ccgccgccag aaccggataa aggcagaact tcaggccgtg
     5641 cttgatgaga aataaaaaaa agcctccatc agagaggagg cagggaaata aggcttaaaa
     5701 ggaaataact tccgcttccg aaaggaagcc agtccactga attatttatc acaataatac
     5761 tgacaactaa agggggacaa gaaaatgaat ggattcagaa acagttcacg gaacggccag
     5821 gtctggcgtt accagcgtgc cggcgggcgg gccgttattc tggaagtcag tggacgctgg
     5881 atggaagcgg cagaagcatg gcgacgggct gcctgtgttg cgcccaggac agactggcag
     5941 cagtttgccc gaaaaagggc tgaacactgc caccggcgct gccggggaag ggtgtaacga
     6001 aaaaagcagc cttcattact ccaggagtca gaaggctgaa aggagtttat gagatttgct
     6061 ttttttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca gagttttttc
     6121 cgtattaagt cgtgatttta tattttgtga ttaatttcac aaaataaggt gttattcagt
     6181 gtgtgctgca atattcagga tgcctgaaat acagatgcta tttatctgat atggagaata
     6241 acatgaggaa atagtaaggc agtatgactt tccgtgagac agatgctggc caacgtggga
     6301 tatttaacgc gggtta
//
LOCUS       DAQSWQ010000172         6214 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.174, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000172 DAQSWQ010000000
VERSION     DAQSWQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.174"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(10..165)
                     /locus_tag="R8G18_004014"
     CDS             complement(10..165)
                     /locus_tag="R8G18_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HEE9797586.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="R8G18_004015"
     CDS             complement(368..775)
                     /locus_tag="R8G18_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEE9797587.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="R8G18_004016"
     CDS             852..1079
                     /locus_tag="R8G18_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEE9797588.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="R8G18_004017"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="R8G18_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1562..2360
                     /locus_tag="R8G18_004018"
                     /pseudo
     CDS             1562..2360
                     /locus_tag="R8G18_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2367..3113
                     /locus_tag="R8G18_004019"
     CDS             2367..3113
                     /locus_tag="R8G18_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEE9797589.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3128..3550
                     /locus_tag="R8G18_004020"
     CDS             3128..3550
                     /locus_tag="R8G18_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HEE9797590.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3608..3964
                     /locus_tag="R8G18_004021"
     CDS             3608..3964
                     /locus_tag="R8G18_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797591.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4133..4798
                     /locus_tag="R8G18_004022"
     CDS             4133..4798
                     /locus_tag="R8G18_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEE9797592.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            5026..5181
                     /gene="hokD"
                     /locus_tag="R8G18_004023"
     CDS             5026..5181
                     /gene="hokD"
                     /locus_tag="R8G18_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HEE9797593.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            5431..5718
                     /locus_tag="R8G18_004024"
     CDS             5431..5718
                     /locus_tag="R8G18_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797594.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            5757..>6214
                     /locus_tag="R8G18_004025"
     CDS             5757..>6214
                     /locus_tag="R8G18_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HEE9797595.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
BASE COUNT         1643 a         1350 c         1690 g         1531 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacgatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     1921 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagaggaa
     1981 gaggaagagg aagaggaaga ggaagaggaa gatctaaata ctgatctccc cctcgccaaa
     2041 aacgagcgtc taaaaaattc gagccggagg ctattgagct gcccgattgg ttgccggaaa
     2101 cactctggca tgagtgggtc cggttcagac aggcattgcg aaaaccgatt cgaacggagc
     2161 agggcgctaa cggggcgata cgggaactgg aaaaattccg tcagcagggt tttacacctg
     2221 agcaggtgat tcgacacagc atcgccaatg aataccaggg cctgttcgcg ccgaaaggtg
     2281 ttcggcctga gacgttgctc cgacaggtta acaccgtctc gttgccggac agtgcgatcc
     2341 cgccaggctt cagggggtaa cggaccatga aaaatattgc gacaggcggc gttctggaac
     2401 gcatccgccg actgaccccg ccacatgtaa ccgccccatt cagaacggta gcggagtggc
     2461 gcgagtggca acttgctgaa ggccagaaac gtagcgagga gatcaaccgc ctgaatcgcc
     2521 agttgcgggt ggaaaaaatt ctgaatcgct caggcatcca gccgttgcac cgtaaatgct
     2581 cgtttgcgaa ttaccaggtg cagaacgacg gccagcgata cgcgttaagc caggcgaaat
     2641 ccatcgccga tgaactgatg accgggtgta caaattttgc gttcagcgga aaacctggta
     2701 ccgggaagaa tcacttagcg gcagctatcg ggaatcgcct gctgaaagac ggtcagacag
     2761 tgattgtggt taccgtggct gatgttatga gcgccctgca cgccagctat gacgacgggc
     2821 agtcaggcga aaaatttttg cgggagctgt gcgaagtgga tctgctggtt cttgatgaaa
     2881 ttggcattca gcgcgagacg aaaaacgagc aggtggtgct gcaccagatt gttgatcgcc
     2941 ggacagcgtc gatgcgcagc gtggggatgc tgacaaacct gaactatgag gtgatgaaaa
     3001 cattgctcgg cgagcgggtg atggatcgca tggtcatgaa cggcgggcgc tgggtgaatt
     3061 ttaactggga gagctggcgt ccgaatgtta gccattcgag ggttgttaag tagtttcagg
     3121 aggatttatg gcgaaacctt ttactcccga acagcgggaa gaactgaaga cgcgaattgt
     3181 ggaactcgtg catcaggacg gtcgggtcac gattcggcag ttgtcagatg aaacaggtat
     3241 cagtcgtgcg tctgtcggtc gcttatgcat agaactggtc gcaagtggtg atgtatataa
     3301 ttctggctac ggcttattcc cgtctgaaca ggctcgcaag gactggcaaa gcgcccgcaa
     3361 aaaactctcg agagtaaagg tgaggaaacc ggttgttgtt gatccggacc ttatctggtc
     3421 attacctgac ggagaaatac gccgctacga caggcgcctg aatataatct gtcgcgagtg
     3481 ccggaagagc gaagctatgc agcgtgtact ggctttctat cagggtaatt ttcaggaggc
     3541 gatactgtga atgaaattag ctatcaggct tcaattaccg ctggcattcg catcaaagga
     3601 gaggagcatg gaaataaaac cagaagatga gttaagcaat atcgttttat ttccggtaaa
     3661 agaggatgac cctcgtaatc aggttaattt tctttatgag ccatcggaaa gaccatattg
     3721 ccatcacgcc tctgtacggg ttgacgaaaa agagcgtcag gtccgctgta aaatctgcgg
     3781 tgcggttgtg gagccgtttg actggatgct ctctgtggca aaaagagaaa ccagactggc
     3841 agatgatgta aggctattgc gccaggagga acaggaaagg cggagaaata tagaaaagct
     3901 gatacagatt gagcgtaacg cgaaagcgcg gatacgcagg gcgacaaaat ccagaactga
     3961 ataattaaat ttagcactgt taaaaattta atccttaacc ggagggattt ctgcaccctc
     4021 aaatcatcag gagaccaccc gaaagggcgg ggagcagtca cacatctgtt tccgatagcc
     4081 ccgttctaat gctacactct ttgatatttt tatgacccca ataaacatat ttatgacagt
     4141 tgctgatttc aaacggccca aattggagct cccaaacggg gcaaacaaac tactactgca
     4201 ctcttgctgt gctccatgtt ccggtgaggt gatggaggcg cttcaggcct cgggaatcga
     4261 ctacaccatc tttttctaca acccgaacat tcatcctcag aaagagtatt taattcgtaa
     4321 ggatgagaat attcgctttg ctgaacaaca cggcgtgccg tttattgatg ctgattacga
     4381 cacagacaac tggtttgaac gagccaaagg aatggaatgg gagcccgaac gagggatccg
     4441 ttgcaccatg tgttttgaca tgcgttttga gcggacagcg ctgtacgccg ctgaaaatgg
     4501 tttcagtgtg atcagcagtt cactgggcat ttcacgctgg aaaaatatgc agcaggttaa
     4561 cgactgtggg cggcgagccg tcgcgcatta tccgggcatg gtgtactggg attataactg
     4621 gcgcaagcag ggcggctcgt cccgtatgat tgaaatcagc aagcgcgaaa aattctatca
     4681 gcaggaatat tgtggctgtg tgtattctct gcgcgatacc aatctacacc gcaaatctca
     4741 gggacgccct cttatcaaaa ttggtcaact ccactacggt aaagaagaga aggagtgatt
     4801 ttatggggca cctttctgat tgatttcata ttggcgaggt aagtagaatg actgcgggtg
     4861 cttgaggcta tctgcttcag gcatgaacac caaaaggcag atagagaaaa gccccagtta
     4921 acattacgcg tccggcaaga cgcttaacat taatctgagg ccaatttcat gctttgcaca
     4981 tgtaggttag cctcttacat gccgaaaggc aaggagaagc aggctatgaa gcagcaaaag
     5041 gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca tagtgacggc actggtaacg
     5101 aggaaagacc tctgtgaggt acgaatccga accggccaga cggaggtcgc tgtcttcgta
     5161 gactacgaat ccaggaagta agagtgaccg ggcggggagc tgatcccatc cccgcccacc
     5221 tctgatgtgt caggcatcct caacgcaccc gcacttaacc cgcccatcgc tgtgatctct
     5281 cagcgtttcg gcgggttttt tgttgtttat ttccggtgaa tttgattcgc gcaccttcgc
     5341 agatagaatc gactcactta agtagcgcgc agggagaaga gggatggacc ccgaacaggg
     5401 gagagctatt tatctggaag gattctgaag atgaaaatcg aagaattgcg tgaaattttt
     5461 agtgaaaatg gcctctatgc tgtgcgcgtt gagaatggag cgattgtcag ccattgccgc
     5521 attagatgtt tgcaatctca acaaaggaag agcggtgctg tgttatttta tttttgtaat
     5581 ggacttctga cggacggttt tattttgcgt gaggacgaat ttgtcacatc attacgggtt
     5641 ttgaaagaga ttggttttaa ggctggtttt ctgcttttgc tgaagaataa actcatctac
     5701 aatcttgagc aggattgaac tcctgctgtg taacaccgtg ccaccggaga aagccgatgg
     5761 cacatataca actggtcaaa caaacctctt ccggattact tctcccggcg acgccggaga
     5821 gttgcgattt tctgcatcaa atcaaaatag gtgagtggat acacgcagac tttaagcgtg
     5881 tgtgtaacta cgcattccac aagcgttttt tcaaactcct gcaactgggt ttcgattact
     5941 ggactccggt cggtggggcg atcacgcctc gcgaacgaaa actggtgtcc ggtttcgttg
     6001 attacctgtg tgaatcagta ggccgggaac atacgccagc tctgagcgaa gccgcagagc
     6061 aatatctgaa tacagttgcg acacgcagaa ccagtgatac ggcattgcta aagtcgtttg
     6121 aggctttccg cgagtgggta gccattcagg ccggatttta caccgagcat ttttatccgg
     6181 acggtagccg tgggtgtcgg gcgaaatcta tcgc
//
LOCUS       DAQSWQ010000173         6203 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.175, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000173 DAQSWQ010000000
VERSION     DAQSWQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.175"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            12..754
                     /locus_tag="R8G18_004026"
                     /pseudo
     CDS             12..754
                     /locus_tag="R8G18_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="R8G18_004027"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="R8G18_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1312..1458
                     /locus_tag="R8G18_004028"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="R8G18_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="R8G18_004029"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="R8G18_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HEE9797596.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="R8G18_004030"
     tRNA            complement(2777..2851)
                     /locus_tag="R8G18_004030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="R8G18_004031"
     CDS             complement(2927..3859)
                     /locus_tag="R8G18_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEE9797597.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="R8G18_004032"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="R8G18_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HEE9797598.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5090..5425)
                     /locus_tag="R8G18_004033"
     CDS             complement(5090..5425)
                     /locus_tag="R8G18_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797599.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5383..5775)
                     /locus_tag="R8G18_004034"
     CDS             complement(5383..5775)
                     /locus_tag="R8G18_004034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797600.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5782..6158)
                     /locus_tag="R8G18_004035"
                     /pseudo
     CDS             complement(5782..6158)
                     /locus_tag="R8G18_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1546 a         1370 c         1374 g         1913 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgcttgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
     5041 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     5101 caaaattaac acctggcgca gatagttgat taatttcatt ttgatatttc gcctggcgat
     5161 tttgaagtct tttttcaacg ttatcttcga gatccttaaa gtctttgtat ttattttcag
     5221 gatggtcaac gtcaactttg ttagcgaata aattagaaat tatttctttt acattgtcgc
     5281 gtctaaattt tgcatcgtcc acttgaaaat tatatcttat tgctcgacct aaacgtttaa
     5341 aaacttcgtt aggttctctc agtctttccc cgttgagctc gtttacaacc tgatgatcgg
     5401 aacctgttgc aaatgattct gccatgatag ctttactgct ttcaaaccac gctgaagcat
     5461 tgctgaagca ttcctggctt tttcactaca atattgggtg agactatcac cattaatacg
     5521 aattcctctt tgaatgtggt agtcgtcctg atcgtagtaa ccgagagata agtaatcatt
     5581 tccgttttta tttttaatta caatagcgag agagttgcct tttttatctt taccgtacca
     5641 ggtaaataag tccgttggtt cgctattccc gctctcatca aattccgtta ccgtatgtct
     5701 gagtttgtgt gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa
     5761 tgcgctgaag atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt
     5821 aatgatagta cggacgagtt ctttgtcttt atagttcgca ggtaattcta ttggttcttg
     5881 gttgtcgata ccaatagtaa tatggtctgt atcattggag ccaatatcaa tataagtaac
     5941 tgaggaatta ttaatattaa atcttaagga tcgtcgttta ctcgtatggc gttttttata
     6001 agctcaagta gatgtttaat acaggtgctt attccttcct taccattaaa aaggtttttg
     6061 agctcttgta ctaatccatt aacttcatca ttgttatttt cattaatgga ttctgggtaa
     6121 gttgcgctgg tattgttgat tgaaatagat ggtagcatga ttttattcca ttttgattga
     6181 tttgttacaa atatatatta cct
//
LOCUS       DAQSWQ010000174         6125 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.176, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000174 DAQSWQ010000000
VERSION     DAQSWQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.176"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1255)
                     /locus_tag="R8G18_004036"
     CDS             complement(<1..1255)
                     /locus_tag="R8G18_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEE9797601.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKILSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="R8G18_004037"
     CDS             complement(1412..1621)
                     /locus_tag="R8G18_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797602.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="R8G18_004038"
     CDS             complement(1644..2354)
                     /locus_tag="R8G18_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEE9797603.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..3193)
                     /locus_tag="R8G18_004039"
     CDS             complement(2870..3193)
                     /locus_tag="R8G18_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797604.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYSDNILVTD
                     SKVVH"
     gene            complement(3372..3572)
                     /locus_tag="R8G18_004040"
     CDS             complement(3372..3572)
                     /locus_tag="R8G18_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797605.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(3572..3844)
                     /locus_tag="R8G18_004041"
     CDS             complement(3572..3844)
                     /locus_tag="R8G18_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEE9797606.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(3847..4125)
                     /locus_tag="R8G18_004042"
     CDS             complement(3847..4125)
                     /locus_tag="R8G18_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797607.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(4126..4683)
                     /locus_tag="R8G18_004043"
     CDS             complement(4126..4683)
                     /locus_tag="R8G18_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797608.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT         1746 a         1273 c         1066 g         2040 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaatcttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcttttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca attgatacac
     3121 cggaatcagc gaaagcctgc accatcatag cagtgtcgct atcaatgaaa atctgtaatt
     3181 catattcgtt catttttaaa atctcctttt taccagacct cctcaatcgc tctgtaacga
     3241 gctaaacagt ctttttcagg ggtagcctgt acagtggtat tacccaccct aaaataagat
     3301 agttttttgc gttagaatgc gattgagagc gtctgataag ctgtttttct aatttctctt
     3361 aaagtctcgg actaccaata acctaattcc gctttatttc gttcaaacca ttccgctgta
     3421 actgctgtct ttagtgttgc gtttgggctg gtggcatgaa tgaagatatc ttttggattg
     3481 caagcatcac cgaaaccacc ttctttgatg agcaggtctg atacatgctt aatcatttta
     3541 tctgtcaaat attcgaattt aatacataac attaaatctc caggagtaaa ttgtttgggt
     3601 ggcaatttct tttattacca tccctgaaga taatcttttt agttgcctga acttcatgat
     3661 gcaaaaacca gatgacatac ttcagataat aatcacgagt gccataacga atcattggat
     3721 taccgtaagc atcatctctt gtcttaacct ctccaccatc aatccattca agcattcctg
     3781 tctcataatt aatcatgaag taagttgcaa agaaccgttt aatatcatct cgccattgct
     3841 tcataatcag acctcctgag acagtttgtg aattttgata cttagtaacc ttgctaaatc
     3901 cagtccaaaa gcatttaagg atttcagact atcaaggtca tcttgcttaa tcctgatatg
     3961 gtgtcttgca gcctctaagc gactattaag caaatcctgt tcgttaatat cacggttaat
     4021 tgcaagcttc atcactgctg agtgtggcag acctgtaatg tttttgattc ggttcagttc
     4081 atcaaaggct gtctttgtaa ctctggttaa tgtgtgttta ttcatttaaa aatcctctgt
     4141 aactaaatag gggtcaaact cgaaatctgg ttgctcgttt tccattggct caatattccc
     4201 aacggcaata tgatgttcaa caatgccaat taatttcgcg cacatcacag caagcaaagc
     4261 gcaatattta tcgcttggta attcgtaact aagtctggtt gcctgtcgct gaagatgatt
     4321 tgtcacggtg gatgcgttaa tttcgtcaag attctttagc agataaaata gttttttgtt
     4381 gtatttcggg tggccttcac ttctacagaa cccaataaaa acatcatcaa gcttgcttgg
     4441 taactgctcg taactgtcaa agttagccag ataattttta atagtattct ttgttgtttt
     4501 gcgtactggc ataaatctgt ctcgtggcgt aatgctacga atgaccgctt caatgttctt
     4561 acgttcggat ttgctaaagg ttgggacttc gtagctggtg gggttgtttg tgtaagcgta
     4621 ggagttgtgc ttgttgcgtt ctcttgatgc ctgaagcatc gctctaaatt ctgaatactg
     4681 cattcatgtt ctgactctca taagatgtta ttattctgtt aacctatata tacaatatta
     4741 gcattttcaa agactaatgt caagtatttt taacattttc gtaacatctg tggtataatg
     4801 ggtttatggg gagagtaaaa tatgaggcgt ggctatgtat ttggtcgcat attttgcatt
     4861 gcacacagac ctcttcaatc gccctgtaac gagttaaacg acctcttcag gtcaactcat
     4921 aaggtcaggt actttttgtg tcagaatgcg attgagagcg tcttacaagg aaaactaatg
     4981 gtaaatctcc ctcctgagct ggggtcacct gatgtatgga ctggtggagg tattgggctg
     5041 attgcaggcc tgctacttcg ctacgtcctc gtcagagtgc ttgttgtctt taaactgttc
     5101 ggtcgtacat ggacgcttga ccttaaggat aacgaccaca cagacccccg ttaaactcct
     5161 tcaaaacccc tgcacgagat gacagataga agtctatggt acaggggtgt ctttagcatt
     5221 ttgtgctatc tattaaaaaa tagcttgaca gaaccggaaa gctgtatcta tcaagcattc
     5281 acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa ttagccatat
     5341 atagagaact gacaagtctc ttcacagaca actctgcaac cttaacagta acttctaaaa
     5401 atgcttattt ttcaacgagt tgtaatcacc caatatataa ggctgtccag taccttcaca
     5461 gataacttca agccctctta accgccccac taatcgggag aagagcgata accccagaga
     5521 gggaagctcc tttcttaacc gacctcttaa acgaccttta cagaccttgt tcaaggtaat
     5581 cacaatagtt tttcttccct cggtttcctg tgttcctcgg ctggctattg agatttgaaa
     5641 gacctctgta agcgctctct tagcgagaga aaaatcattc atagtgaccc caagcgaagc
     5701 gcccctctaa aaaaagattt tttagctatt ttgtttaaaa gcatatcaaa cgatatattt
     5761 tattgattta ggtcaaagac aaaagcattg acaaaatggt aagattgttc acatgacctt
     5821 acttatttaa gagactttga aacagcctgt ttaacaggtc actacctctg gtcttatcgt
     5881 tcccttcttt ctaggttaag agtttttcag tttttattct taagataatt gttaagatga
     5941 tactctccct tcgggttata tcgtatctct tctcccgtta aatatccctt cacagtcaga
     6001 ctaaataaca cctgtgtagt tcttggtaga atctttcaag gtaacttagc tctgagttta
     6061 tgaagttgac ctatgagata gactctaccg aatctgttag tcaattgttg cttaggtgct
     6121 gctaa
//
LOCUS       DAQSWQ010000175         6121 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.177, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000175 DAQSWQ010000000
VERSION     DAQSWQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.177"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..358
                     /locus_tag="R8G18_004044"
     CDS             <1..358
                     /locus_tag="R8G18_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEE9797609.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="R8G18_004045"
     CDS             complement(398..1069)
                     /locus_tag="R8G18_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEE9797610.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="R8G18_004046"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="R8G18_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HEE9797611.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="R8G18_004047"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="R8G18_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HEE9797612.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /locus_tag="R8G18_004048"
     CDS             2798..3211
                     /locus_tag="R8G18_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEE9797613.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="R8G18_004049"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="R8G18_004049"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEE9797614.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="R8G18_004050"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="R8G18_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HEE9797615.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="R8G18_004051"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="R8G18_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEE9797616.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="R8G18_004052"
     CDS             complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="R8G18_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEE9797617.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1497 a         1609 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gcgataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 a
//
LOCUS       DAQSWQ010000176         5931 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.178, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000176 DAQSWQ010000000
VERSION     DAQSWQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.178"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            292..368
                     /locus_tag="R8G18_004053"
     tRNA            292..368
                     /locus_tag="R8G18_004053"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="R8G18_004054"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="R8G18_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HEE9797618.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="R8G18_004055"
     CDS             complement(1357..2247)
                     /locus_tag="R8G18_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HEE9797619.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="R8G18_004056"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="R8G18_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HEE9797620.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="R8G18_004057"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="R8G18_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEE9797621.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1561 c         1549 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg ccctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAQSWQ010000177         5865 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.179, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000177 DAQSWQ010000000
VERSION     DAQSWQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.179"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..560)
                     /locus_tag="R8G18_004058"
     CDS             complement(<1..560)
                     /locus_tag="R8G18_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797622.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
     gene            complement(704..1462)
                     /gene="aroD"
                     /locus_tag="R8G18_004059"
     CDS             complement(704..1462)
                     /gene="aroD"
                     /locus_tag="R8G18_004059"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEE9797623.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="R8G18_004060"
     CDS             complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="R8G18_004060"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HEE9797624.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(2371..3636)
                     /locus_tag="R8G18_004061"
     CDS             complement(2371..3636)
                     /locus_tag="R8G18_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9797625.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3863..5077)
                     /locus_tag="R8G18_004062"
     CDS             complement(3863..5077)
                     /locus_tag="R8G18_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9797626.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(5177..5533)
                     /locus_tag="R8G18_004063"
     CDS             complement(5177..5533)
                     /locus_tag="R8G18_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HEE9797627.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5669..5776)
                     /gene="rprA"
                     /locus_tag="R8G18_004064"
     ncRNA           complement(5669..5776)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="R8G18_004064"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1690 a         1339 c         1330 g         1506 t
ORIGIN      
        1 cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga gatattcttt gttatcaaac
       61 tcgaccagtt taatcaggtc ttctttcgtg acttcattca gtttgccgcc ttgctggcgt
      121 ggatcgacaa atgtcccgat gccaatatca ctaataatac caggctggtg ggctgcggcg
      181 gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat aagcaataat tttattttgt
      241 tctgcgagtt cagaaatacg cggcgattgt ccccagtgac cacataatgc ccatttcacc
      301 agaccttctt gcgccagagg actaatacca cggtcggcgc gatcgccaag ccctgtcgga
      361 ctaataatcg ataaattacg tggcgtttga gtctgtttat atttatcagc aagagctgta
      421 attaacgtgg tggcttccag aataccgccg ccagcgccta acacacaaag tgttgcttcg
      481 tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc attaatacga
      541 ggtggtttta caggtttcat tgttaattcc tgattcagat gtgattttcc ctacgccaga
      601 tggagaatta gcgcacagag actcacgaaa tatttttatt aaatacggtt aaatatattt
      661 tttaagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc tggtgtaaaa
      721 tagttaatac cgtgcgtaaa tcatttaccg agatttgccc tggtgcagag gcttttttta
      781 ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa attacgccag
      841 tttttgccat cgacatcgtg atgattggac gatcggcata ctgctcctgc atctccaggg
      901 tcgcggtaag caacgtcagc acatcgctgg tactttgcgg catcagcgca atcttaggaa
      961 tatcggcgtc gaaggactgc attttgcgca gacgggcaat gatttcttcg gcttccggcg
     1021 ttttatggaa gtcatggttg gacatgacaa ctttcacatc atgcgcgtgg gcgtaggcga
     1081 cggtttcttt aacctgatca tcgccggtaa ataactccag atcgatcata tcaaccaggc
     1141 cgctgtcgat ggctgcacga ttgagagcaa tataagcctc ggtggaaatc gcctgctcgc
     1201 cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg gtttcacgga
     1261 gaatttttgc cgccgccatg acagactcca cattggagag gtcggcaaag tggtccacac
     1321 gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat ttcacgcggg
     1381 cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgcacca atgacgagat
     1441 cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc tgtcaggcac
     1501 cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc cataacgtga
     1561 actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg caacctgctt
     1621 gttgtgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca gtgaccagca
     1681 gtcccggatg taacagactg atatcattaa ccaatgattc attctcaagg gggttcatac
     1741 ctacttttgt gccattggtt aaaatgtcgg cggaagccag gacttcagca aaggcttgct
     1801 gatcggcgag atcggtaacc gtgacgacac aatcggtgtt ttcgttaacc cgctgcgcga
     1861 aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt tcttttaaac
     1921 cttcaattgc cccctgcgcg ccaatcgctg ttgaggcacc tccggcccct aacagcacca
     1981 tcgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt
     2041 cggtgttata gccacgcaga tagccatcat cattaacgat ggtgttgatg gcacccacca
     2101 gtttggctgc tggcgttaat tcatcaacat attcacacgc cagttgtttg ttcggcatcg
     2161 acacaccagt tccgcgcatt ttgagggctt ttaatccttc aattgctccg ggaaagctat
     2221 cgttatccac ttcgaaggcc atataggtaa atggcaatcc cgctttttct aaggctttat
     2281 tctgcatttc aggcgataaa ctgtggcgga taggataagc catcaaccca atcaattcgt
     2341 attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg tgtttaaccg
     2401 ggtgctaaat ttgcgttcgc caaaccgcac atcattctca ggaataataa aaacgcggta
     2461 atagcggcta aaaacaataa ttgcggtaat cagggccagc agcgcgaaag taaaatcgag
     2521 aacaatgata tattgcaggc cgatgttcga cagataaccg gtaatcagcg gaataacaaa
     2581 gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc ttttggggaa
     2641 aaactctgac atgaccgaaa caccgagctg taaaatgccg ccagctgctg agaaaccgat
     2701 aacaaaggcg ccggcattac acaccagtgg agaagggtac aggtagataa tggcagctgt
     2761 tattgttgcc agcgcagagt taaatacatt agcccagatg ggccggacca tttttttcag
     2821 tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag agatggtttt
     2881 taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca tccacaccac
     2941 aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa caccttccag
     3001 ccagactaac ggtttgctgt tcatttgcgg taattcatta gctacgctgg cgtccaccaa
     3061 ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaaca gaatacccgg
     3121 aataatcagc ccatagccgt accagatatt attgagcaac atatagctca ccagcattgg
     3181 gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg aaccagaggc
     3241 tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg agttagcgat
     3301 acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg gaataccaaa
     3361 gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac caaacttatc
     3421 ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac cggaaattaa
     3481 ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc agagatgtca tattttgggc
     3541 aagcgtaata acactaatgc cgtgcaggaa atagctgaag taaatacaaa gaacagccag
     3601 gataaatggc gtgctgaaag ccttattttg agacattttc acagctcctt gctgaaagat
     3661 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     3721 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     3781 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     3841 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     3901 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     3961 cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
     4021 tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac
     4081 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
     4141 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
     4201 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
     4261 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
     4321 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
     4381 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
     4441 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
     4501 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
     4561 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
     4621 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
     4681 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
     4741 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
     4801 tggtctgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
     4861 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
     4921 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
     4981 gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg taattaaaat
     5041 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     5101 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     5161 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     5221 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     5281 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     5341 tggcatgaag atgttttttt cgttgctgac gcatagccaa cagttgctcg actacacaat
     5401 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     5461 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     5521 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     5581 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     5641 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     5701 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     5761 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     5821 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctacc
//
LOCUS       DAQSWQ010000178         5640 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.180, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000178 DAQSWQ010000000
VERSION     DAQSWQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.180"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="R8G18_004065"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="R8G18_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEE9797628.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="R8G18_004066"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="R8G18_004066"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEE9797629.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="R8G18_004067"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="R8G18_004067"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HEE9797630.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="R8G18_004068"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="R8G18_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEE9797631.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAQSWQ010000179         5619 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.181, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000179 DAQSWQ010000000
VERSION     DAQSWQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.181"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..513)
                     /locus_tag="R8G18_004069"
     CDS             complement(<1..513)
                     /locus_tag="R8G18_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HEE9797632.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="R8G18_004070"
     CDS             complement(759..1211)
                     /locus_tag="R8G18_004070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEE9797633.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="R8G18_004071"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="R8G18_004071"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEE9797634.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="R8G18_004072"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="R8G18_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HEE9797635.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="R8G18_004073"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="R8G18_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="R8G18_004074"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="R8G18_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HEE9797636.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5619)
                     /locus_tag="R8G18_004075"
     CDS             complement(5494..>5619)
                     /locus_tag="R8G18_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEE9797637.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1467 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtg
//
LOCUS       DAQSWQ010000180         5614 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.182, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000180 DAQSWQ010000000
VERSION     DAQSWQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.182"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="R8G18_004076"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="R8G18_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEE9797638.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="R8G18_004077"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="R8G18_004077"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HEE9797639.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="R8G18_004078"
     CDS             2475..4124
                     /locus_tag="R8G18_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEE9797640.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="R8G18_004079"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="R8G18_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1255 a         1271 c         1457 g         1631 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAQSWQ010000181         5609 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.183, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000181 DAQSWQ010000000
VERSION     DAQSWQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.183"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(1..1133)
                     /locus_tag="R8G18_004080"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="R8G18_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /locus_tag="R8G18_004081"
     CDS             complement(1133..1315)
                     /locus_tag="R8G18_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797641.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="R8G18_004082"
     CDS             complement(1327..3060)
                     /locus_tag="R8G18_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEE9797642.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="R8G18_004083"
     CDS             complement(3057..3551)
                     /locus_tag="R8G18_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEE9797643.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="R8G18_004084"
     CDS             complement(3673..4023)
                     /locus_tag="R8G18_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEE9797644.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="R8G18_004085"
     CDS             complement(4066..4530)
                     /locus_tag="R8G18_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HEE9797645.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="R8G18_004086"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="R8G18_004086"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HEE9797646.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="R8G18_004087"
     CDS             complement(5024..5239)
                     /locus_tag="R8G18_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HEE9797647.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1255 a         1623 c         1369 g         1362 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tcccgccccc cgacagacct gtgctatcgg aaagaaaaaa
     5281 ggccatcagc aaaactctga tggcctgaat cacctttacc aatattgtat gaaaaaacac
     5341 gcacgactaa ttgacaataa ttttcatttc cattaaaaaa gatcgcgtag cattcttaat
     5401 tcatgaggaa ctttacccgc cagcaatctg agtagcgaaa gctgttcatc cccaatgttt
     5461 ttgctggcgg gtcctttttt ctacggtcct cctccccgga ggggggcatt tcattatttt
     5521 tctaatcgtt actggtgaga aggcggaaga aaaagccagt tcttcgcccc taaataacag
     5581 catgattcca gtccatgcaa aatctgatt
//
LOCUS       DAQSWQ010000182         5541 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.184, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000182 DAQSWQ010000000
VERSION     DAQSWQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.184"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1369)
                     /locus_tag="R8G18_004088"
     CDS             complement(<1..1369)
                     /locus_tag="R8G18_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEE9797648.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITAPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="R8G18_004089"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="R8G18_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEE9797649.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="R8G18_004090"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="R8G18_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEE9797650.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="R8G18_004091"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="R8G18_004091"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEE9797651.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="R8G18_004092"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="R8G18_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HEE9797652.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1409 a         1450 c         1305 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg agccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAQSWQ010000183         5527 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.185, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000183 DAQSWQ010000000
VERSION     DAQSWQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.185"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="R8G18_004093"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="R8G18_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEE9797653.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="R8G18_004094"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="R8G18_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HEE9797654.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="R8G18_004095"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="R8G18_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HEE9797655.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="R8G18_004096"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="R8G18_004096"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HEE9797656.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="R8G18_004097"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="R8G18_004097"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HEE9797657.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="R8G18_004098"
     CDS             4447..4812
                     /locus_tag="R8G18_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HEE9797658.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="R8G18_004099"
     CDS             4962..5480
                     /locus_tag="R8G18_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEE9797659.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1391 c         1241 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAQSWQ010000184         5485 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.186, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000184 DAQSWQ010000000
VERSION     DAQSWQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.186"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..115)
                     /locus_tag="R8G18_004100"
     CDS             complement(<1..115)
                     /locus_tag="R8G18_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEE9797660.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="R8G18_004101"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="R8G18_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HEE9797661.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2333..2968)
                     /gene="nth"
                     /locus_tag="R8G18_004102"
     CDS             complement(2333..2968)
                     /gene="nth"
                     /locus_tag="R8G18_004102"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEE9797662.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="R8G18_004103"
     CDS             complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="R8G18_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HEE9797663.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="R8G18_004104"
     CDS             complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="R8G18_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HEE9797664.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="R8G18_004105"
     CDS             complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="R8G18_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HEE9797665.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5350..>5485)
                     /locus_tag="R8G18_004106"
     CDS             complement(5350..>5485)
                     /locus_tag="R8G18_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HEE9797666.1"
                     /translation="RKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
BASE COUNT         1444 a         1391 c         1349 g         1301 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 gccgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacggggggg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gaggggggtg
     1981 atggcttaac ggcgatatgt tctgctattt ggcgttttta ctagtgagat agtccagttt
     2041 ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa
     2101 ttattttaac ctgattcttg caatgttttt acattgcgac agcatcgtga tcttgatcac
     2161 gaatgaatag aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca
     2221 aacggattac ctgatgataa cgctaagcaa tggcatcatt cacgttaaaa agatgtttta
     2281 aaagaggata aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt
     2341 caactttctc tttgtattca caaagatctt caataataca agagccacag cggggcttgc
     2401 gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact
     2461 ctgccggaac cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa
     2521 attgagtacg attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga
     2581 atgcagtgtt taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg
     2641 cagcacgatc ttccggaacc tcgccattat gctgctccag taagatacgg caggttttga
     2701 tgatattttc tgctttgctg ttataaagcc caatcgtttt gatataggtt ttaacccctt
     2761 caacgcccag ttcaagcatc gctgcaggcg tattcgccac cgggtaaagt ttcgccgtcg
     2821 ccttattaac actgacatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa
     2881 aaggcgaact gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga
     2941 ggatctccag gcgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc
     3001 acgttcggca gctgcttcag tacggcgctt tttcattctt tcatcaatca ggtattttcc
     3061 tgccagcatc agtcccaggc caataaatgc acctggtggc agcatcgcca gcaggaaagg
     3121 ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc
     3181 tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat
     3241 ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt
     3301 tttggcggcg aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat
     3361 aaaaatccct aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc
     3421 gctgaccacc gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca
     3481 gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc
     3541 taaacccaga gcgttagtgg cagtggacgt gaccgccaac agaggacaaa ggccgagcaa
     3601 ctggaccagc gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct
     3661 cacgctttat tctccacagg caggaagttg agaaagttgt gccggtaacg tctgagcgta
     3721 caatccggcg cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa
     3781 ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat
     3841 ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc agttcgattt tatcgccaag
     3901 ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc
     3961 tcccaccagc agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc
     4021 ggctaccggt ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc
     4081 tggcgcagtt accagatagc aactctgtgc cagcgtattg ttatagcgtt cggctggcag
     4141 cacctgatca aataatgcct tttgttgcag actggcctgt tcagcaatcg tcgttttggt
     4201 catctggttg atggccgcag ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat
     4261 gccgtgtttt cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg
     4321 acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac
     4381 gccgtcagga tagccgccga aactgcggat caaccagact aataagcccg caagcgcgcc
     4441 gaaaataaga cgaccacgat tggtcgtaga agcggtaacc gggtcagtca aaataaagaa
     4501 tgcgccgagc atggtcgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg
     4561 tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat
     4621 atgccagcga atcgctttct gccatagcaa ccacaggccg ccagccagcc aggcgagatt
     4681 tacccattgc caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg
     4741 ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta tccagcggtg tcgcctgact
     4801 aatgccatca atacctaagc gtagtgtgtt catatcacca ccactggcgg tatgcccgct
     4861 gaaaataacc tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg
     4921 taaccagctg gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc
     4981 cggattaaac gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa
     5041 caccgtaccc agcacgacca tccaccatgg cgcgaggggg ggaatactta ccgccagcaa
     5101 taagcctgtc agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag
     5161 tttgagtacg agagcttcgg ctaacagagt actaaccgat gccaacagga tctgaacgag
     5221 agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac
     5281 caacagcata atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct
     5341 gaataccatt tagtcctcgt ttacaacctt ctgctgggcg gcttttttgg cctgagcgcg
     5401 ggcaatagcc gcggcaactg ccgctttgcg cggatcaacc tgttcttctg gttctgcgtt
     5461 agtctgttgc tgttccagct tgcgc
//
LOCUS       DAQSWQ010000185         5153 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.187, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000185 DAQSWQ010000000
VERSION     DAQSWQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.187"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            117..554
                     /locus_tag="R8G18_004107"
     CDS             117..554
                     /locus_tag="R8G18_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HEE9797667.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGR
                     REFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
     gene            635..1054
                     /locus_tag="R8G18_004108"
     CDS             635..1054
                     /locus_tag="R8G18_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HEE9797668.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
     gene            complement(1279..1602)
                     /locus_tag="R8G18_004109"
     CDS             complement(1279..1602)
                     /locus_tag="R8G18_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HEE9797669.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1886..2080
                     /locus_tag="R8G18_004110"
     CDS             1886..2080
                     /locus_tag="R8G18_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HEE9797670.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
     gene            complement(2922..3059)
                     /locus_tag="R8G18_004111"
     CDS             complement(2922..3059)
                     /locus_tag="R8G18_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HEE9797671.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISV
                     Q"
     gene            3107..3448
                     /locus_tag="R8G18_004112"
     CDS             3107..3448
                     /locus_tag="R8G18_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HEE9797672.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIV
                     ISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLGLFISIKNN"
     gene            complement(3445..5010)
                     /locus_tag="R8G18_004113"
     CDS             complement(3445..5010)
                     /locus_tag="R8G18_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="HEE9797673.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKSFQEAEQRRKEIE
                     KEQAETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRL
                     SSSIHARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLR
                     ARAGDEMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKT
                     AQTNLLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAF
                     EKYKDVLNKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDI
                     LKIKDTGDWKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFID
                     KNKLNTINEVLGI"
BASE COUNT         1457 a         1291 c         1066 g         1339 t
ORIGIN      
        1 aaaaaaacag ccacccacac cacgtgctat ttccacccga tgccacaaaa accagcacaa
       61 acattaccgt tctcagacct cattatgttt tactgaaacc atgagatgag acacctatgg
      121 gacactgtaa ctttatggca tggcacacac tccgggacgc actgaaaatg actggaagat
      181 cgcgttcaca gttttaccgt gatatgcgct caggtcttgt cagttaccgt accagccagg
      241 acggacgacg ggagtttgaa accagtgaac tgatccgggc atacggcgaa ttaaagcgga
      301 atgagacacc agaaaggcac actcaaggac actcagaaaa acctcatgat cagcagacag
      361 aacgcattct ccgggaactg aatgagctga aacaatgcct gacgctgatg cttgaggata
      421 aacaagccca ggatacggag cgccggcgcc aggaagcaga acggaaacag ctacagaatg
      481 agatagccca gctcaggcag gctctggagc aggaaaagaa acggggattc tggtccaggc
      541 tgttcggtca ctgaacgcgc tcagaaactg atgataagat aatcttcgga taataactca
      601 ccgagaataa atactttaag gtagggagac actcatgaga cgtaccggaa acaaactttg
      661 tcttatcgcc atgataacag caacagtagc tctcacagcc tgtaccccaa agggcagcgt
      721 ggaacaacat acccggcatt acgtatatgc ttctgatgac ggttttgatc ccaacttttc
      781 cacccaaaaa gccgacacaa cacgaatgat ggtgcctttt tttcggcagt tctgggatat
      841 gggagctaaa gataaagcga caggaaaatc acggagtgat gtgcaacaac gcattcaaca
      901 gtttcacagc caggaatttt taaactcact ccggggcaca actcaatttg cgggtactga
      961 ttaccgcagc aaagacctta ccccgaaaaa atccaggctg ctggctgaca cgatttctgc
     1021 ggtttatctc gatggctacg agggcagaca gtaagtggat ttaccataat cccttaattg
     1081 tacgcaccgc tgaaacgcgc tgaacgcaat tctgacgcaa ataccgcaaa aagagaggtt
     1141 tagccccgaa aaaacaggaa ggatgcgtca ggatcgtttt cagctcactg catagctatg
     1201 catgaaagcg aatggtgatc actttgggag cttacggtgt tcataccgtc tgttttcgac
     1261 agttttctct ccgggaagct aatctgccat aagcctggat aacagggcac ggtgatactc
     1321 cgtaatagcg atcagcacct ccgcatactc cctgtccccg acacgcttgc cgtcgataaa
     1381 gagttttttc tgtaacgccc ccagtctcat aacttctttc agtacccgat catcagtcag
     1441 tgagttaact ttcttaccga gagctgccgc ccgtaaaaaa ccggaaacgg tcttgccgca
     1501 ttcagcagcc ctttttctga tttcctggtc ttcttctgcc gtcagacgaa cagggcgact
     1561 gatagcccgc ctgcgcgtat tacttccact tcgttttgtc ataaaacatg gctccgtatc
     1621 tgacatgggt gtcggggcaa agccctgacc agggtaattg taatagcgtg catgtatgcg
     1681 cggtataaca attacacatc ctgtcctgtc agcaagttcg aattaccgac aaaggcacgg
     1741 cagttttttg gcagtgacag tattgtcagg cagaaatacc cgtcaaagcg tcagacggca
     1801 gaataccggc ggtaaaacag tcgaaaagtg gtacgcgata taatcacaca acgcagatga
     1861 cagacagaac ggagaaaacg aaaagatgaa caagcagcag caagccgtac tcaacatggc
     1921 aggattcata aaaagccaaa gcctgacgct gctcgaaaag ctggacgcac tcgatgctga
     1981 cgaacaggct accatgtgtg agaagctgca cgaactcgca gaagaactcc agaacagcat
     2041 ccagacacgc tttgaagtag aaaatagcac agagagataa cgctcaggac aggatgccga
     2101 acggcacaac agagcgtaag gagaaaatgt cacataaggc gctcttccgc ttcctcgctc
     2161 actgactcgc tacgctcgga cgttcgactg cggcgagcgg tgtcagctca ctcaaaagcg
     2221 gtaatacggt tatccacaga atcaggggat aaagccggaa agaacatgtg agcaaaaagc
     2281 aaagcaccgg aagaagccag cgccgcaggc gtttttccat aggctccgcc cccctgacga
     2341 gcatcacaaa aatcgacgct caagccagag gtggcgaaac ccgacaggac tataaagata
     2401 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2461 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2521 ttggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2581 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2641 acacgactta gcgccactgg cagcagccgt tggtaacttg gttagcagag cgatgatggc
     2701 gcaaatggtg caacagagtt cttgaagtag tggcccgact acggctacac tggaaggaca
     2761 gtatttggta tctgcgctct gctgaagcca gttacctcgg ttaagcagtt ccccaactga
     2821 cttaaccttc gatcaaaccg cctccccagg cggttttttc gtttacaggc agcagattac
     2881 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac agagatcccc
     2941 gtcaatttag aagaagatga tggagccaca gtccctccct gaacatcacg tatatagtta
     3001 gcctgacatc caacaaggag gtttatagcg aatattccca caaaaaatct tttcctcata
     3061 actcgatcct tataaaatga aaagaatata taacgaggtt taatttatga gtttaagata
     3121 ctacataaaa aatattttgt ttggcctata ctgcgcactt atatatatat accttataac
     3181 aaaaaacaac gaagggtatt atttcctagc gtcagataag atgctatacg caatagtgat
     3241 aagcactatt ctatgcccat attcaaaata tgctattgaa cacatatttt ttaagttcat
     3301 aaagaaagat tttttcagaa aaagaaaaaa cctaaataat gcccccgtgg caaaattaaa
     3361 cctatttatg ctatacaatc tactttgttt ggtcctagca atcccatttg gattgctagg
     3421 actgtttata tcaataaaga ataattaaat acccaacacc tcatttatag tattaagttt
     3481 attcttatca ataaaggcac atagaatgcc tgtaacaata gcaatccccc agatacctaa
     3541 tgtagttcca gcaagcacac taaaaagtaa aacaacaaca taactaactc cagcatctac
     3601 agctttcttc tctaatgtaa gaaatagcgg cttccagtca cctgtatcct taatttttag
     3661 gatatcagat accacatcat atccaaaaga aacatgtccc gtaatcttca agtacttggc
     3721 aaactgatct aaatgcttag cccagtcttc atacttaacc gcctccagtg cattgaaaat
     3781 tgcatcacgg tctgctttgc tgaatttctt atttaataca tccttgtatt tctcaaaagc
     3841 agctagagct tcattcacat tgctgatttt cttaccttta gatttatcag caagttcctg
     3901 tgccattttt gaatattttt caccatattt ttcagatagc gtttgataaa agctaactgt
     3961 tgcatccaca gcatccttaa tctgcgagtt caggagatta gtctgtgctg ttttcaaatt
     4021 atcttcagca tcatgaatac gtgaaactgc tgtacttcgc ttattattag cctgagaaat
     4081 agcctcttgg attccattta tctcagagct aatctggtta agacgcgttt ctgatgctgt
     4141 tacctgcttt tgtttttcct ccatctcatc accagctctc gcccgtagtc tggtggcctc
     4201 aaaaaaagga cgactctgaa gcggatcatt cgccctagga gataaaagtt taaccctttc
     4261 atccagttct ttatatttag cagatgcctg atccaactca tttcgttttc cggacagcgt
     4321 attcgtttct gcatcacggg catgaatact ggagcttaaa cgggtattga gcgtattaat
     4381 ctcttcatcc actttcgcca gctcagattg tgcagcagca agattttttt gtgccacctc
     4441 cacagcccgg gcctcttcac tcaatgctgc caggcgtttc tcttcatcct cagccagttt
     4501 caattggcgt tctgtttcag cctgctcctt ctctatctct ttacgtcgtt gttctgcttc
     4561 ctgaaaagac ttttccgcag cttccgcttc tttacgggct ttttcttctg cttttgcaag
     4621 gcgcaaccgc tctgcttctg cctgcatcgc tgcattattg gcatgagcca ggtcaataac
     4681 ctccggatga gtggaattat ggcgaagcgc ctcattcaca atatccttca gatgttgagt
     4741 cagcgcatcc cggtttgctt ttgctttagc ctgtgcttct gcggcagctt ttgctcgggc
     4801 agcctgttct gcctgcgttt tcttcaattg agcagtagac catttagctg tggcatgaat
     4861 ggctgcagaa ctttcacttt tgctacctcc agttccacca ccaccgccag agccactccc
     4921 gtctggatta ccattcaaaa gagtaatgat tacctgcccc ttatcatcat aaggaacacc
     4981 atctttatag tacgctacag ctgtttccat tataaaatcc tctttgacat taaaaacaaa
     5041 tagttaaaag caatatactg tatatataat cactggtttt atatacagtt taaaattacg
     5101 ccgctgcatt ttccctgtca accctgtgga ttttcatttt tgtgaaaacg atc
//
LOCUS       DAQSWQ010000186         5071 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.188, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000186 DAQSWQ010000000
VERSION     DAQSWQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.188"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            144..259
                     /gene="rrf"
                     /locus_tag="R8G18_004114"
     rRNA            144..259
                     /gene="rrf"
                     /locus_tag="R8G18_004114"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            274..349
                     /locus_tag="R8G18_004115"
     tRNA            274..349
                     /locus_tag="R8G18_004115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:307..309,aa:Thr,seq:ggt)
     gene            389..504
                     /gene="rrf"
                     /locus_tag="R8G18_004116"
     rRNA            389..504
                     /gene="rrf"
                     /locus_tag="R8G18_004116"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(567..1325)
                     /locus_tag="R8G18_004117"
     CDS             complement(567..1325)
                     /locus_tag="R8G18_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEE9797674.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1333..2436)
                     /locus_tag="R8G18_004118"
     CDS             complement(1333..2436)
                     /locus_tag="R8G18_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEE9797675.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2446..3627)
                     /locus_tag="R8G18_004119"
                     /pseudo
     CDS             complement(2446..3627)
                     /locus_tag="R8G18_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3695..4727)
                     /locus_tag="R8G18_004120"
                     /pseudo
     CDS             complement(3695..4727)
                     /locus_tag="R8G18_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1390 a         1303 c         1224 g         1154 t
ORIGIN      
        1 agcgatgcgt tgagctaacc ggtactaatg aaccgtgagg cttaacctta caacgccgaa
       61 gatgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
      121 gcggtctgat aaaacagaat ttgcttggcg gccgtagcgc ggtggtccca cctgacccca
      181 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
      241 gagtagggaa ctgccaggca tcaaatttag cgtgctgata tggctcagtt ggtagagcgc
      301 acccttggta agggtgaggt ccccagttcg actctgggta tcagcaccac tttttaggtt
      361 aaagttcggc agattagaaa agaatttgtc tggcggcagt agcgcggtgg tcccacctga
      421 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtag ggtctcccca
      481 tgcgagagta gggaactgcc agacatcaaa tcaagcgaaa ggccatccga aaggatggcc
      541 tttttgcttt tcgaactaac attcaattaa tggattacct gcgataaaaa tgccctcgta
      601 cgctctgatt taggatgcgc aaaaaattca tcaggtgcag cttgctccac tatttcccca
      661 cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa accccatctc atgcgttaca
      721 cacaacattg tcatacccga ttgcgccagc ccaatcatcg tatccagcac ctctttcacc
      781 atctccggat cgagcgccga cgttggctca tcaaacaaca taattttcgg cttcatacac
      841 agcgaacgcg caatggcaac gcgttgctgc tgaccaccag aaatttgtcc gggaaactta
      901 tgcgcatgtt cggcaattct cacccgctca aggtaatgca tcgccagagc ttcagcctct
      961 ttctttggca tcttgcgtac ccaaatcggt gccagggtac agttctgtag aacggtcaga
     1021 tgagggaaga gattgaaatg ctgaaagacc attcccactt cctgcctgac gcgctcaata
     1081 ttgcggatat cttcattaag ttcgatgcca tctactacga tccgtccctg ttgatgttct
     1141 tccagatgat taatacaacg aatggttgtc gatttaccgg aaccagaagg gccacacaga
     1201 acgatccgtt ctcccagttg cacggttaaa tttatatttt tcaaaacatg gaattgtcca
     1261 taccatttat tgacgttttc cagcgtaatc atcgcgttag caggttgcag taaaatttgg
     1321 ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa cgtttttcca gatgctggct
     1381 atagcgcgac atgctgaaac agaagatcca gtagatcagt gcggcgaaaa catacccttc
     1441 cgtcgacata cccaaccagg cgggatcaac ggttgcctgc tgaacgctac tgaaaagatc
     1501 gaacaacccg ataatgatca ccaggctggt atctttgaag agtgcgatga tggtatttac
     1561 cagcccagga attaccagct tcaacgcctg tggcagaata accagcccct gagttttcca
     1621 gtaacccaat gccagtgact ctgccgcttc atattgccct ttaggcagcg cctgtaatcc
     1681 gcctcgcacg acttccgcaa catatgctga ctgaaacagg atcacgccaa ccagcgcgcg
     1741 gatcagtttg tcgatattgg tgccttctgc cataaacaaa ggcagcatga ccgaagacat
     1801 aaacaatacg gtaatcagcg gtacgccgcg ccagaactcg ataaaaatga ccgataagat
     1861 acgcacaatc ggcatatggg agcggcgacc taacgccagt aagatccccc acggtagcgc
     1921 ccccgcaatc ccaacggatg caataattaa agttagcgtc agcccgcccc attgccgggt
     1981 ttcaacccgc tccagaccaa aaaaaccgcc atacatcagc caccagacaa tcagtgggta
     2041 aatcaccgcc cagaccgcaa tatagcgacc gcgatgcggg agtattttcc agaacattgg
     2101 tgcgattgac acaaggccaa tcagtaatgc caggttaatt cgccagcgtt ggtcgtgtgg
     2161 gtaaagtcca tacataaact gaccaaatcg ttcgtggatg aagacccaac aggcaccggc
     2221 ttttgtgcag tcggcacgcg ttgagccaac ccaattagcc tgcaaaaatg cccagttcag
     2281 caacggtggg atcagttccc acatcaacca tatgcagcca atagtcagca ggctattgct
     2341 ccagctggag aacagatttt ttcgcaccca caccatcgcg cggcttgagt tatggctcgc
     2401 tgggcgcgag ggctgagaca gcaatacttt tgtcatcatg actccttagc gttcaacgat
     2461 cgcgatgcgg cggttataga tattcatcag cagcgagata gtcaggctga taatcagata
     2521 gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc gtctggttca gcacggtgcc
     2581 agcaaacagc gaaaccatat cgggatagcc aatagcggcg gcaagagagg agtttttgac
     2641 gatgttgaga tactggctgg ttaacggcgg aataatcact cgcagtgcct ggggaataat
     2701 gacctagcgt agcgtaaccg ggttgggtaa tcccagtgac cgagccgctt catgttgacc
     2761 ataaggcact gcctggatcc cagcgcggat aatctcggcg ataaatgcag atgtataaac
     2821 cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa accatcccgc cgcggaagtt
     2881 aaagcctcgt agggctggga catcccagtg tagtgctgcg ccaaaaagcc attgcgccag
     2941 taaaggcaaa ccaatgatca acactgcggc gatcggccag gtgcggcgca gttgtccggt
     3001 ctttatctgg tatgttttat taaagcggaa tagcccaaca gaaaggacta tagccataac
     3061 aacagccagg ataaacgcaa taaatccgtc tcccagctgc ggtgacggaa tataaagccc
     3121 acgattgctc aaaaaggcca gatcaaacgc gctcacagcc tggcgtggtc cgggcaaatt
     3181 gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc gggggaatat tacggaagat
     3241 ctcaatataa attgtggaaa gctttcgtag cagccagtta tccgaaagtc tcgccagacc
     3301 gataaagaag cccaaaacag aagcgaacac aatacacaat gcagaaacca gtagcgtatt
     3361 gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc tgctggtaat cgatcaaatg
     3421 ctggacaata ccgaagccag cgccgcgatc cagaaaggca aaacctgaag taatgccacg
     3481 attattgaga ttcgttacag tgttgtgaaa taaccaacca acaatgccga caacagcaac
     3541 aacggcaagg atctggaata accaggcacg aaccgtaggg ttggcaaaag agagtgagcc
     3601 tttaacggtt gagcggcgat gagacataga aacctcggaa agcataacgc tgaagagagc
     3661 gccgccgtag cgacgctcgt tacctcacac cgacttaacg cacgggcggc gcgtactgaa
     3721 taccgccgtt attccagaga ttattttgcc cacgtttaat tttcagcggg ctttctgaac
     3781 ctacgttacg ctcgaaaatt tccgagtagt tacccacctg tttgatgatg ttataggccc
     3841 atttattatc cagcttcaga tccttgccgt aatcgccttc tttacccagc agatgtgcca
     3901 tatcaggcgt tgctggatta gccgcttttt catcgacgtt ctgggaattg atgcccatct
     3961 cttcagcatt cagcattcag catggcgaaa agcgtccagc gtacaatcga gaaccattca
     4021 tcatcgccac gacgaactac cggcccaagc ggttctttag agataacctc cggtaagaca
     4081 atccattcag ctgggttgct taatttgatg cgcagggcat acagttgcga ctgatccgag
     4141 gccagcgtat cgcagcgacc agattccagt gccttcgctg attcgtcaga gcgatcgaaa
     4201 gtcaccggtg tgtacttcat attgtttgcc ttgaaatagt cggcgacgtt gagttcggta
     4261 tctgtacccg cctgaataca gacggtagcg ccatccagtt ctttcgcgct ttttagcccc
     4321 gctttatcgt gcgtcaggaa gccaatgccg tcgtaatcag tgacgccagt aaatgccatt
     4381 cccatcccgg catcgcgaga tgaagtccag gtcgtattac gggagagcaa atccacctcc
     4441 cctgactgta aagcggtgaa gcgttctttt gcagtgagcg gggtatattt cactttcgtg
     4501 tcgtcaccaa atacagcagc ggcaacacca cgacaaacat ctacatcaat acctgaaaac
     4561 ttaccgtctg catcggcata agagaaccca ggtaatccat cactgatccc gcattgcaca
     4621 aaaccttttt tctgtacggc atcaagcgtc gcgccagcat gcgcctgatt tgcaacggca
     4681 agcagcacgc tggcggcagc cagtgtggct atcatcatct ttttcataat gcttcctgtg
     4741 aggcgaaaat tatcgttata ggacgtttgg gaaacgtctt ttacctgtct gtggccatcc
     4801 tcttataagc aaaagtagtg ccatcttttg cgcacgattt cgcgtcgcaa taagctgagt
     4861 gtagacagga aataataaaa acagcgcccc gcaatggtgc gaaaatgaaa catgcgccac
     4921 attttggcgc aaaatattta cgcgtgtttt actaagcaga ataatgattg ttagcgtgag
     4981 tatatttctg gtgggaggtg tgaaagttaa attgatgcgt tatttcgaaa gcagcgaaat
     5041 aacgcgatat aacatgctaa ggtaatgact c
//
LOCUS       DAQSWQ010000187         4844 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.189, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000187 DAQSWQ010000000
VERSION     DAQSWQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.189"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(34..954)
                     /locus_tag="R8G18_004121"
     CDS             complement(34..954)
                     /locus_tag="R8G18_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEE9797676.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="R8G18_004122"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="R8G18_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HEE9797677.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="R8G18_004123"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="R8G18_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HEE9797678.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="R8G18_004124"
     CDS             complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="R8G18_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HEE9797679.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1308 a         1081 c         1140 g         1315 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gacccaaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgcat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa tttgagttta tagttttcta taacataatt aata
//
LOCUS       DAQSWQ010000188         4832 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.190, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000188 DAQSWQ010000000
VERSION     DAQSWQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.190"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(108..551)
                     /locus_tag="R8G18_004125"
     CDS             complement(108..551)
                     /locus_tag="R8G18_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEE9797680.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            708..1637
                     /gene="metA"
                     /locus_tag="R8G18_004126"
     CDS             708..1637
                     /gene="metA"
                     /locus_tag="R8G18_004126"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEE9797681.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1906..3507
                     /gene="aceB"
                     /locus_tag="R8G18_004127"
                     /pseudo
     CDS             1906..3507
                     /gene="aceB"
                     /locus_tag="R8G18_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3537..4828
                     /gene="aceA"
                     /locus_tag="R8G18_004128"
                     /pseudo
     CDS             3537..4828
                     /gene="aceA"
                     /locus_tag="R8G18_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1206 a         1271 c         1224 g         1131 t
ORIGIN      
        1 gcagaaggcc atcctgacgg atggcctttt tgcattggcg cagaaaaaaa tgccgggtgc
       61 gacgctggcg cgtcttctcc agcctgggca ggcacggcag gggcagctta ttcctccaca
      121 tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta
      181 accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg
      241 gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag
      301 ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac
      361 ccaaccggct gatcccgttc attaaccgcg acccacaacg gcgcttccgg caggaaggaa
      421 cgaactagca cctccagctc ggcccgatac tctgctgata aaaaatcgtg tgtggcatcg
      481 acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata
      541 ctaataacca ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga
      601 aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct
      661 atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg
      721 tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa
      781 cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc
      841 cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg
      901 tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc
      961 tgaacaactt ctactgtaac tttgaagata ttcaggaaca aaactttgac ggtctgatag
     1021 ttaccggcgc gccgctgggc ctagtggagt ttaatgatgt cgcttactgg ccgcagatca
     1081 aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg
     1141 tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct
     1201 ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg
     1261 attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt
     1321 acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagta
     1381 aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc
     1441 aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc
     1501 cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt aatttgctgt
     1561 ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta cggcacatga
     1621 atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct
     1681 caggcacctt cgggtgcctt ttttatttcc gaaacgcacc tcagcaagtg aataaatttt
     1741 attcatattg ttatcaataa gttatcaagt atttttaatt aaaatggaaa ttgtttttga
     1801 ttttgcattt taaatgagta gtcttagttg tgctgaacga aaagcgcaca acgatccttc
     1861 gttcacagtg gggaagtttt cggatccatg acgaggagct gcacgatgac tgaacaggca
     1921 acaacaaccg atgaactggc tttcacaagg ccgtatggcg agcaggagaa gcaaattctt
     1981 actgccgaag cggtagaatt tctgactgag ctggtgacgc attttacgcc acaacgcaat
     2041 aaacttctgg cagcgcgcat tcagcagcag caagatattg ataacggaac gttgcctgat
     2101 tttatttcgg aaacagcttc cattcgcgat gctgattgga aaattcgcgg gattcctgcg
     2161 gacttagaag accgccgcgt agagataact ggcccggtag agcgcaagat ggtgatcaac
     2221 gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg aagattcact ggccccggac
     2281 tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg cagttaacgg caccatcagc
     2341 tataccaatg aagcaggcaa aatttatcag ctcaagccca atccagcggt tttgatttgt
     2401 cgggtacgcg gtctacactt gccggaaaaa catgtcacct ggcgtggtga ggcaatcccc
     2461 ggtagcctgt ttgattttgc gctctatttc ttccacaact atcaggcact gttggcaaag
     2521 ggcagcggtc cctatttcta tctgccgaaa acccagtcct ggcaggaagc ggcctggtgg
     2581 agcgaagtct tcagctatgc agaagatcgc tttaatctgc cgcgcggcgc catcaaggcg
     2641 acgttgctga ttgaaacgct gcccgccgtg ttccagatgg acgaaatcct tcacgcgctg
     2701 cgcgaccata ttgttggtct gaactgcggt cgctgggatt acatcttcag ctatatcaaa
     2761 acgttgaaaa actatcccga tcgtgtcctg ccagacagac aggccgtgac gatggataaa
     2821 ccattcctga atgcttactc acgcctgctg attaaaacct gccataaacg cggtgctttt
     2881 gcgatgggca gcatggcagc gtttattccg agcaaagatg aagagcgtaa taaccaggtg
     2941 ctcaacaaag taaaagcgga taaagcgctg gaagtcaata acggtcacga tggcacatgg
     3001 atcgctcacc caggccttgc ggacacggca atggcggtat tcaacgacat tctcggctcc
     3061 cgtaaaaatc agcttgaagt attgcgcgaa caagacgcgc cgattactgc cgatcagctg
     3121 ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc gcgccaacat tcgcgtggct
     3181 gtgcagtaca tcgaagcgtg gatctccggc aacggctgcg tgccgattta tggcctgatg
     3241 gaagatgcgg cgacggctga aatttcccgt acctcgatct ggcagtggat ccatcatcaa
     3301 aaaacgttga gcaatggcaa accggtgacc aaagccttgt tccgctagat gctgggcgaa
     3361 gagatgaaag tcattgccag cgaactgggc gaagaacgtt tctcccaggg ccgttttgac
     3421 gatgccgcac gcttgatgga acagatcacc acttccgatg agttaattga tttcctgacc
     3481 ctgccaggct accgcctgtt agcgtaaacc accacataac tatggagcat ctgcacatga
     3541 aaacccgtac acaacaaatt gaagaattac agaaagagtg gactcaaccg cgttgggaag
     3601 gcattactcg cccatacagt gcggaagatg tggtgaaatt acgcggttca gtcaatcctg
     3661 aatgcacgct ggcgcaactg ggcgcagcga aaatgtggcg tctgctgcac ggtgagtcga
     3721 aaaaaggcta catcaacagc ctcggtgcac tgactggcgg tcaggcgctg caacaggcga
     3781 aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt agcggcggac gctaacctgg
     3841 cggccagcat gtatccggat cagtcgctct atccggcaaa ctcggtgcca gctgtggtgg
     3901 agcggatcaa caacaccttc cgtcgtgccg atcagatcca atggtccgcg ggcattgagc
     3961 cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg gaagccggtt
     4021 ttggcggtgt cctgaatgcc tttgaactga tgaaagcgat gattgaagcc ggtgcagcgg
     4081 cagttcactt cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg ggcggcaaag
     4141 ttttggtgcc aactcaagaa gctattcaga aactggtcgc ggcgcgtctg gcagctgacg
     4201 tgacgggcgt tccaacccta ctggttgctc gtaccgatgc cgatgcagcg gatctgatca
     4261 cctccgattg cgacccgtat gacagcgaat ttattaccgg cgagcgtacc agtgaaggct
     4321 tcttccgtac tcatgcgggc attgagcaag cgatcagccg tggcctggcg tatgcgccat
     4381 atgctgacct ggtctggtgt gaaacctcca cgccggatct ggaactggcg cgtcgctttg
     4441 cacaagctat ccacgcgaaa tatccgggca aactgctggc ttataactgc tcgccgtcgt
     4501 tcaactggca gaaaaacctc gacgacaaaa ctattgccag cttccagcag cagctgtcgg
     4561 atatgggcta caagttccag ttcatcaccc tggcaggtat ccacagcatg tggttcaaca
     4621 tgtttgacct ggcaaacgcc tatgcccagg gcgagggtat gaagcactac gttgagaaag
     4681 tgcagcagcc ggaatttgcc gctatacctt cgtatctcac cagcaggaag tgggtacagg
     4741 ttacttcgat aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac
     4801 cggctccact gaagaatcgc agttctaagc aa
//
LOCUS       DAQSWQ010000189         4701 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.191, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000189 DAQSWQ010000000
VERSION     DAQSWQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.191"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            387..1469
                     /locus_tag="R8G18_004129"
     CDS             387..1469
                     /locus_tag="R8G18_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HEE9797682.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="R8G18_004130"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="R8G18_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HEE9797683.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="R8G18_004131"
     CDS             complement(2127..2267)
                     /locus_tag="R8G18_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HEE9797684.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="R8G18_004132"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="R8G18_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HEE9797685.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="R8G18_004133"
     CDS             complement(2623..2913)
                     /locus_tag="R8G18_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HEE9797686.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="R8G18_004134"
     CDS             complement(2906..3076)
                     /locus_tag="R8G18_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797687.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="R8G18_004135"
     CDS             complement(3076..3531)
                     /locus_tag="R8G18_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HEE9797688.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="R8G18_004136"
     CDS             3796..4173
                     /locus_tag="R8G18_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797689.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
     gene            4170..4598
                     /locus_tag="R8G18_004137"
     CDS             4170..4598
                     /locus_tag="R8G18_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797690.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT         1350 a         1049 c          980 g         1322 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagccaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcagtgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatggcactc
     4201 aagtcagtgg tgaagtaaga gatctagtaa ttcttcttga aactgatgga aaaacggtgt
     4261 ctgattattt gcagccaatc cctcccaaaa aagatacaca ctggagcatg attttcattc
     4321 cggtagcaat ttatatacta cttaatcttg tatacattat tgctcaccca ttatttcctg
     4381 aaaaatatct aaaaacatca acatctatat ttctattcat ttccttcata ttaagtgcaa
     4441 tagtagcata ttttgttttc cataagcata aatctgccac tctatccata tgcagcttag
     4501 tgttcttagt gttaattgta ggtgctaacg tagggtttat cagttacagc gacctaacac
     4561 aaaaagcgac gcaaaaatta gatagtttgt ctaaataatt atgttgccat gcgaagtatg
     4621 catggctgca tgtctgcctt ccatttaaaa tgggctaaga cctataaccc taaatattat
     4681 tctttattat cttctttacc a
//
LOCUS       DAQSWQ010000190         4519 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.192, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000190 DAQSWQ010000000
VERSION     DAQSWQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.192"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..264)
                     /locus_tag="R8G18_004138"
     CDS             complement(<1..264)
                     /locus_tag="R8G18_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797691.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAP"
     gene            493..1101
                     /locus_tag="R8G18_004139"
     CDS             493..1101
                     /locus_tag="R8G18_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEE9797692.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1199..2590
                     /gene="selA"
                     /locus_tag="R8G18_004140"
     CDS             1199..2590
                     /gene="selA"
                     /locus_tag="R8G18_004140"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEE9797693.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2587..4431
                     /gene="selB"
                     /locus_tag="R8G18_004141"
     CDS             2587..4431
                     /gene="selB"
                     /locus_tag="R8G18_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEE9797694.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1017 a         1143 c         1330 g         1029 t
ORIGIN      
        1 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
       61 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca ggggattgac
      121 cggatggccg gacgtcactc cgccggggca caccgaacag gccacaccgg tgggggcacc
      181 gatgcgcacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg
      241 acgcgctgcc ggttttccgc tcatctgctc atccttgttt tatcgggtta tttgtctgct
      301 tccaggcaaa accctacaga tttataagaa agccttaagt gagatatcca tcagatagaa
      361 acaattagga aaatatttat atcaccatga taaatatgtg atttatttgg aagtaacaaa
      421 ccatcaacat tttctgccct ttttcccatc accaggccat actgatattt ttcctcgcac
      481 aaaggactcg gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct
      541 attctgttgt tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg
      601 gacaacggcg tggcgcaatt taacccgtta ggaaaagtgc cggtgctggt gaccgaagag
      661 ggcgaatgct ggtttgattc gccgatcatc gctgaatata ttgagttaat gaatgtcgct
      721 ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcgctg
      781 gcggatggca ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccggcggcg
      841 cagcagtctg aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg
      901 ctggaaggat atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc
      961 gccattgcct gtgctgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc
     1021 gaccgcccac acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgctcgc
     1081 accgaaccgc caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc
     1141 cgtggttatg ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat
     1201 gacaaccgaa acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga
     1261 tagctccttc ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg
     1321 tcagatgctc gacgaagcgc gagaagtgat tcgtgacagc cagacgcttc ctgcgtggtg
     1381 tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa gaggcgcaga gcgcgctgcg
     1441 tccggtgatc aacctgacgg gaaccgtgct gcataccaac cttggacgag ctttacaggc
     1501 ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga
     1561 tctggacgac gccggacgcg gacatcgcga tcgggcgctg gcgcaactgc tgtgccgtat
     1621 tacgggggcg gaagatgcct gtatcgtcaa taacaatgcg gcggcggtgt tattgatgtt
     1681 ggcggccact gccagcggga aagaggtggt agtatctcgc ggcgaattgg tggagattgg
     1741 cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc tgcaccctac acgaagtagg
     1801 gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact
     1861 gttgatgaaa gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagatga
     1921 agcggaactg gtggcgctcg gcaaagagct ggatgttccc gtagtgactg atttaggcag
     1981 tggctcgctg gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt
     2041 gattgcggcg ggcgtcagtc tggtgagctt ctccggcgac aagttgttag gcgggccgca
     2101 ggcagggatt atcgttggta aaaaagagat aatcgcccgc ctgcaaagcc acccgctgaa
     2161 gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta
     2221 tttacaccct gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc
     2281 agaggtcatt caaatccagg cacaacgttt acaggctccc cttgccgcac attacggcgc
     2341 ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt
     2401 tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct
     2461 tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg atgattggtc gtatttatga
     2521 cggacgattg tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt
     2581 gttgaaatga ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag
     2641 gtgattactg gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc
     2701 gatctcggct atgcctactg gccgcagccg gatggtcgcg tgcctagttt tatcgacgta
     2761 cccggtcatg aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg
     2821 ctgttggtgg tggcgtgcga tgacggcgtg atggcgcaga cccgtgagca tctggcgatt
     2881 ttgcagctga ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac
     2941 gaagcgcgtg ttgatgaggt tgaacgccag gtaaaggagg tgctgcggga atacggtttt
     3001 gctgaggcaa aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc
     3061 gagcatctgc ttcagttacc aggacgtgaa cacgccagcc aacatagtgt ccgcctcgcg
     3121 atcgaccgcg catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc
     3181 ggggaagtga aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta
     3241 cgtgcgctgc atgcacaaaa ccagccaaca gaaaccgccc atgccgggca gcgtatcgcg
     3301 cttaacatcg cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc
     3361 gatgcgccgc cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacgccgctg
     3421 acccagtggc agccgctgca tattcaccac gccgccagcc acgttacggg acgcgtttcg
     3481 ctgctggaag ataaccttgc cgagctggtg ttcgacacgc cgctgtggct ggcggataac
     3541 gatcgcttag tgctgcgcga tatctcggcc cgcaacacgc tggcgggggc gcgtgtggtg
     3601 atgcttaacc cgccgcgtcg cggtaaacgt aagccggaat atctgcaatg gctggcgtct
     3661 cttgcacggg cgcagagcga tgccgatgcg ttatctgttc atctggaacg cggcgcggtt
     3721 aaccttgcgg atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaattgctg
     3781 caacagcctg gctatattca ggctggttat agcttgttga atgcgccggt tgccgcccgc
     3841 tggcagcgga aaattctcga cacattagcg acttatcatg agcaacatcg cgatgaacct
     3901 ggtcctgggc gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctggta
     3961 ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg
     4021 catctgccgg atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca
     4081 gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc
     4141 gacgagcagg caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg
     4201 atcgtcaaag atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc
     4261 gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta
     4321 ggccgaaagc tggcaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt
     4381 cgtggaaatg atcatttatt gcgcgacgca ttattatttc cggaaaaata aggaaatgat
     4441 taattaattt ttaaaataaa ttaatacaaa tttctggtaa tgactccaac ttattgatag
     4501 tgttttatgt tcagataat
//
LOCUS       DAQSWQ010000191         4480 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.193, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000191 DAQSWQ010000000
VERSION     DAQSWQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.193"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(7..1068)
                     /locus_tag="R8G18_004142"
     CDS             complement(7..1068)
                     /locus_tag="R8G18_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HEE9797695.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="R8G18_004143"
     CDS             complement(1065..2462)
                     /locus_tag="R8G18_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HEE9797696.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="R8G18_004144"
     CDS             2617..3615
                     /locus_tag="R8G18_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEE9797697.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4480)
                     /gene="ompG"
                     /locus_tag="R8G18_004145"
     CDS             complement(3726..>4480)
                     /gene="ompG"
                     /locus_tag="R8G18_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HEE9797698.1"
                     /translation="EPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVH
                     YQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWL
                     SMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFST
                     QEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGL
                     SVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1227 c         1099 g         1062 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaatggattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg
//
LOCUS       DAQSWQ010000192         4413 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.194, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000192 DAQSWQ010000000
VERSION     DAQSWQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.194"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(175..2124)
                     /locus_tag="R8G18_004146"
     CDS             complement(175..2124)
                     /locus_tag="R8G18_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEE9797699.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAAHAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2209..2544)
                     /locus_tag="R8G18_004147"
     CDS             complement(2209..2544)
                     /locus_tag="R8G18_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HEE9797700.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2544..2900)
                     /locus_tag="R8G18_004148"
     CDS             complement(2544..2900)
                     /locus_tag="R8G18_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HEE9797701.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2900..4396)
                     /locus_tag="R8G18_004149"
     CDS             complement(2900..4396)
                     /locus_tag="R8G18_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEE9797702.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1313 c          970 g         1109 t
ORIGIN      
        1 ttaacgttac taataaatcg tgaaacaatc tcctcatttg ttagtatttt cgcctgagca
       61 cagcaagagg ttaatattgc aaccgtcatt aaaaatggct ttatcacaaa acaaactcca
      121 ttgttagcat gttacgcgat aattttgcct catctttcac cacaaaaaaa gaactcacct
      181 tttaaatgga gaatacccaa catcatgggt gattttcata aagggatcgc ctgttttcgg
      241 caaatcgata acgcgcattc caggcggcgc gttgtcaaac gtcaccttaa gttcgctgcg
      301 ggttgccggt gaagatgcaa ggcttaacag ctgattcctt tccagtgata cattcggggt
      361 gtagcgttta tcgggagata aatacccatc accacgtggc atctgccatc cggtcatgtc
      421 atagacaaaa tcatggaact gtttgcccca ctcgtcgaac ttatcattca gaccaaatcc
      481 actgttcagt gcatcagcca caaaattttt aacaagccag ggatgtgctt tttcaaactc
      541 ctgcgccatc atgcccagtt caagcaatcc accaatcaga ctgatctttc cgaggccctt
      601 taatttcagc cctgtttttc ctgccgccgt tgtccattga ttctgggcga ctgttgccgc
      661 acgaatactt gccaccgcct tcacaccaat atacagggca gaaaccgttg ctattgtttt
      721 tactgcctgt tcccatcccc ccattgctct gacaacctga tcaacctcct gccagacttt
      781 tttcacaacc ggaccgaccg tttcccagtt atcaattatg agatatgcac caccgacgag
      841 aagcgcaatt aatccctttg ccggcgtcat attcatcacg ctacccatga tttttgttat
      901 gcgtgtcaat gtgccaatcg ccacgcccat cgtcagtaat gccgcaccag ttttcgcgat
      961 ggtttttacg acctcaggat tttctctgac aaacgtgcgc acttcttcca gaaaaggctt
     1021 catctctttt atgccttcat tgagtgaagg caggaaggtt tcccccagcg tggaagaaat
     1081 cgcattgatc tggttctgca gcagcaacaa ctgattttct gtcgtcgcgg catgggcggc
     1141 atattctttc tgcattgagc cgccatactg ctgcgcatcc gccacacgat taaagttggt
     1201 tcgcaacaaa tccagattcg tgagcagcgg cgcaatggcc cccagggatt ccttcccgaa
     1261 cagcgctttc agcacagcag cctgtttttc tttcggcact tttgccagag aatccagtac
     1321 gtgcagcatg gccccgcggg cgtctttctg catatccgcc gccagttttt tcgggctaat
     1381 gcgcaacgag cggagcactt ttttctgcga tttggtggca gaatccctcg ctgtcagcga
     1441 caacataaaa ttctttatcc ccgtcgcagc aatttctgac tccaccccca ttccggcaat
     1501 ggttgccccc attgcggcaa tctctccgga ggccacaccc gcaacactgc ctaaagggcc
     1561 aatacgggtc acaacatcag aaatcttttt cgcacttgca ggaccggtat taccaagata
     1621 gttaatctta tccgcaagcc ctgccacctc tccctgtgtc agtttaaagg cagtgcgcca
     1681 ctgcgccatc atctgaccgg attcttctgc cgtggtgtca aaggccacgc ccatcttcac
     1741 ggcatcgtca gtaaactgca tcagctcgtc acgggcgata ccagcctgac cacccgccgc
     1801 cacaatttcg gcgatacctt ccgccgacat gggcagttct gttgacaggt cgcgcacctg
     1861 ctccgtcatt gccttaaacg cttccggcgt atccagacca tccaccactt tccggacatc
     1921 cgccattttt gattcaaggg caatggcaga tttgaccggg agcgccagcg cccccagtac
     1981 tgcggttccg gcacccgctg cgcccagtaa caggctggca aactcctttt taaaaccttt
     2041 cagctggcgc tgcattccct tcagcggtgc cgatacctta tccacggcag tgatgatcgc
     2101 cttcagctga aaactgtcag ccatgcttca tctcctcgtt aatgcggacg gcctcggctt
     2161 ccagttctgt aaactgcgaa acagcccccc gtcgcagctc cagtggattc agtttccaga
     2221 accaggcaac attgtagagt cgtttccgga ggtgcttccc gtctccgact gggtaaaaaa
     2281 acgcaggatc tgcatgctgg tcttaaaaat atccagtttt gccatctgcg ccgccgatga
     2341 acgcgggatc cccgccagca acgggatata tttcagtgcc acctggctgt ccagtttaat
     2401 actgccttca ccggaaataa tgaaaggaaa acccagcgcc tcgatttcgt catacgtggg
     2461 ctcgcgcaac tccagcacat gcagcatttc gttatgcgct gtcaccggtt tttttaaaac
     2521 tatttctgta acgttcattg ccattactga taccctccct cttcgccgtg gaattcaaga
     2581 tctgccgtgc cttcttcggc attatgatta gcctctccgt gcagccatgc cgccgaaagc
     2641 acatacacca tgccgtttgc cagctcagcg gtaatcgtca tctggtctga ggtggtaatt
     2701 ttgtcgaccg gaaagttttt gggcacttta aacgtgccct taacgtaagg tgaccgccag
     2761 gtctccttgt aatccacatc ccctgccatg ccgacgatgt catcccggac attggtgttc
     2821 atcggcactt caatgccacc tgtcagcgat aactgctgac catccacttt aaaaaaacag
     2881 gttccggcga tctttgccat tatgctgact cctctgaata ctgaagacgg aactggttaa
     2941 ccaccgcaaa gacacgtaac tgattaacat aatccggcgg gaacagcgta ttcagtcggt
     3001 tcgggttatc tgcatcacgc tcaactatca gatactgttt aaacagatcg taattttcca
     3061 caataccggc acgctccatc tgacgatatg tcgccagaag ttcccctttg ataacggcag
     3121 gagtgacaat cgcctgcccc ggaccaaaac gggtaccatc atttgccagc ttgtgacgtc
     3181 cgtacttgct tgtgatgacc gatttcagtt tgcgaagaac atatgcactg gtatgcagag
     3241 tttcactgtc cagatagctg ttgtctgcca cgccatacgc attctttttg taggtggtta
     3301 cggaacgctg gatctgcaac gtgccgcctt ccacataagc cgtcgccacg ccgtgagata
     3361 aaagggtctg ctgctctgtc atgatgaatc gcttaccttt cggtgccgga agcatcccca
     3421 ccagctcccc cgtctgtgtc ggacgggcag gatcattccg gataaatacc gcttcacggg
     3481 caaggcgact ggcaaccagt tcatcgacag gcgactgggt ttctttttcg taaccggcaa
     3541 gcgtgatatg ttgctgatta tgcatatctc cggcaccaac cagctctgac agcgttccca
     3601 gttttgcggt atagacatgc ccgtataact ggcgcgcata actccagcga ccgctgctgt
     3661 cattcatttc ggtcatcatc atattgatgg aggcggcatc gttgaacggc agaccgataa
     3721 agtcgaatgc ctcatcgccc atagcagcaa cagcggcggt aagatcaggc gctccgctac
     3781 cggcagcacc gacttccgtc accacccgaa gccctgctgg cagaatctca ccaccaccag
     3841 aaccataata attcagacag accggtaact cattaccata cagcccctta tgacgggcag
     3901 tcagcgtcac cacacctgca tcagatgacg ccgtaaacgg cagggtgata accccgttta
     3961 ccgcttcctt aatcgcggtg gcaaccgcag tggcatcatc gccattcacc acaggcacct
     4021 gtacacggga gcgcccgaca tacagactca gggtgccgct ttcctctgct tctccggcaa
     4081 ccgtcaccct gaccgtcgcc gccgcccctc tggcttccgg tactgcaata acatacagtt
     4141 caccgaaagg atctgtctga cggtagactt ccaccatacg cgccagctgg ctccccgccc
     4201 cacaaatctg gcgggcataa tctgccgatg gcatcagcac caggctgtta acctcaatgg
     4261 ctgcatcgtt gctggcatgc ccgatcagta atgcaggcgc gctggtcacc gctgtatttg
     4321 ccgcagaatt gttcatctcg gcgtagaaca acggcaccag cgtattcgac ggaacagtat
     4381 taaaacttat cgtcatgatt ttttagcctt atc
//
LOCUS       DAQSWQ010000193         4395 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.195, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000193 DAQSWQ010000000
VERSION     DAQSWQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.195"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..590)
                     /locus_tag="R8G18_004150"
     CDS             complement(<1..590)
                     /locus_tag="R8G18_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEE9797703.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="R8G18_004151"
     CDS             complement(583..993)
                     /locus_tag="R8G18_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEE9797704.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="R8G18_004152"
     CDS             complement(999..1532)
                     /locus_tag="R8G18_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HEE9797705.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2586)
                     /locus_tag="R8G18_004153"
     CDS             complement(1510..2586)
                     /locus_tag="R8G18_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HEE9797706.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2583..3974)
                     /locus_tag="R8G18_004154"
     CDS             complement(2583..3974)
                     /locus_tag="R8G18_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HEE9797707.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4021..4227)
                     /locus_tag="R8G18_004155"
     CDS             complement(4021..4227)
                     /locus_tag="R8G18_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797708.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1052 a         1281 c         1039 g         1023 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcgc
     2041 cgtcgccgct ttacctgttc cgggttttcc cagaaccagt cgcccgcatt catcgtcata
     2101 agccagcgtc tgaacctgcc ccagtaaccg gttaagacaa tcggcaacgg tttcgccgtg
     2161 ttccggctgg gcatcaatca ccgctgtctg cggcacgcca gcatcaacaa cggtgatgcc
     2221 aaatggcgca gccagttcac tgactattct gagcaggttt tttccgctct gctggagtgg
     2281 caaagcagag cagtcaacca gatcggctgt tttgcttcgc ccgacaattc ccatgctgac
     2341 gctgctggcg tcataacgaa gcggtagtgc ctccacatat ccggtgagca caggctcatc
     2401 cccgataagc acttcaacca gctcaccatt ttttatccgg ggttgataat cccggcttcc
     2461 gggccagcgg gtggtaatgg caacattaaa atcccgggca atacggttaa tgcccgcact
     2521 gatacggacg gatgtccagc cgccccattc gcgaccggaa acccgaagta aaacggtatc
     2581 attcatctga cgggtaccct taatgccctg accggaacaa agcccggatg agagatggca
     2641 tttcgatcca ggatatcagt ttcacgggac gcatcgtcgt accatgacgc agccagaaca
     2701 agcgcaggca gaacttccgc tggcgttcgc tctgcggttt cctccgtctg aaccagacgc
     2761 gcctgaatat cccggttcag ttctgtacgt aatgacgtca gctgaaaaaa cagcctgtca
     2821 tccgccgtac gcctgagctc ctgttcaatc gccgcgttca gcgattcgcg tatgatggtg
     2881 agattttccc gcgtgggtgg ttgcgctctc tcatcctgtc ctgtgctggc tgccacgctg
     2941 tcaagtgccg gatgagaaac gtgaataata tcggactgac gttcagtgga accaccaaca
     3001 gccacaaccg cctgctgatt tttcaccagg cttccgggtt gtggcagtga agtgaccgcc
     3061 cttgtggctt cgctgattgc cgtcgtccgg atgacagcag ccacaaggtt tgtctgctgt
     3121 ttttgcctga taaccgatgc ggaatctgtg ggccacaccg cacgcggtgc cagtcctggg
     3181 tccagcgtaa tgccggacat tgtcgttatg gactgcacca gatcctgtgt gttatccacc
     3241 agccgggttc cggcccgcca ggtatcctgc aacgtatgca caaaatcact ggcaaccgac
     3301 ggcggcatca gaatgacgga taaatcgccc tgcagtagtc tcgctccggc agaaacagcc
     3361 gaattaacca ttttgaaagc tgtctgaacg gttcccagca tatcggtcgt ccgggcaata
     3421 acatcgttct gaataaagtc cggtattccc gcgagatcaa aatcgccgaa catgtcttca
     3481 atcaactcat ccaggaatac ggacgactcc tccagttttc tggcggttgc agctccggcg
     3541 accggaaatg acagttcccc gctctcaaca aactgaaatg aaacccggca catacggcct
     3601 tcagtgccgg agtgagaaac ggtcacctgc ccgtcaatac agccctgcat ttcaccgaac
     3661 tgcggatgga tcagcgtccc cggcccggcg gtttcaatcg ctgtaatcag cctgtcgcgc
     3721 tgctctgcgt aatcatcacc aacgagatac gcattaatcg tcagccgtcg cgtggcacgc
     3781 ccgagatctt ccgtgtacgg tttgtcacgg tttgggtatt catggacctg aacgcggcgt
     3841 ccgaacgaac cctcgtcgct ctccaccgaa aacggaacgc cacgaaatga agcgtcgtaa
     3901 agattatcgc gccaattgat acctgaagat gcagaaaaga gtgaagaaag agaaggtaaa
     3961 gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc ggcttatata
     4021 ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca ctcccgactc
     4081 gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta caaaaaactg
     4141 gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa aatcatcatc
     4201 tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg caaaatcaaa
     4261 tacacctata cttttgccga attcataaga agcttttctc aatataatcg caccgctggc
     4321 agaaggggat gggcacgata tcctcagttg tctgaatcca tctgtatcca agtcaacgtc
     4381 attatctgca gatgt
//
LOCUS       DAQSWQ010000194         4326 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.196, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000194 DAQSWQ010000000
VERSION     DAQSWQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.196"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..579)
                     /locus_tag="R8G18_004156"
     CDS             complement(<1..579)
                     /locus_tag="R8G18_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEE9797709.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(642..1091)
                     /locus_tag="R8G18_004157"
     CDS             complement(642..1091)
                     /locus_tag="R8G18_004157"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HEE9797710.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="R8G18_004158"
                     /pseudo
     CDS             complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="R8G18_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1026 a         1089 c         1167 g         1044 t
ORIGIN      
        1 gttcgggatc atcgcgtcaa cggtgatggc gtcagcaggc tgtacgccgc caaccagcgc
       61 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
      121 ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
      181 caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc cgaggaaggc
      241 atacgaagca taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca ggtttacgaa
      301 ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccatgc
      361 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
      421 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagatc
      481 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
      541 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgagt
      601 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
      661 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
      721 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac ggtttctccg
      781 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
      841 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
      901 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg gtaagtcaat
      961 gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
     1021 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
     1081 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
     1141 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
     1201 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
     1261 tggagccgag gccaagcagc tggtgatcga ggttcagggt gatgtcatca ctgcgctgca
     1321 gctcgttaca gtgctgggca gcgtggatgt tttcctaggt atagtgccag gcgctgaagt
     1381 taattgggcg ctgcggaacc accagcagac cgttaccgtg gcggttagtc agtgccgtcc
     1441 agcggacatg ctgacggtta ccgttgttct gcgggaaggg atagttctcg aacatggcat
     1501 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
     1561 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaattccc atggtgaaac
     1621 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
     1681 cctggccatc ggcagcgatg cgccagatgt aggtgcagcg cattccgaaa tcaaacaccg
     1741 gcggggcaat aaccgtgcgg ctgatgatca acacttcacc ctcgctctgt tctacggcaa
     1801 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
     1861 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
     1921 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
     1981 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
     2041 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
     2101 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
     2161 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
     2221 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
     2281 cctctgcgtg cagggtgtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
     2341 actcgccgcg agtcagatcc cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact
     2401 ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
     2461 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
     2521 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
     2581 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
     2641 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
     2701 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
     2761 aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg cggatgttac
     2821 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgcg
     2881 cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
     2941 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
     3001 caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg
     3061 tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg acgcgatcca
     3121 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
     3181 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
     3241 gttgtggtac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
     3301 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
     3361 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
     3421 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
     3481 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacggtga
     3541 aatcgttaat atgtgttagt tgttttccga tcagataaac atcgcggaag atccccgctg
     3601 accaccacat atcctggtct tccacgtagg tagagtccgc ccattgcatc acgcgcacac
     3661 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
     3721 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
     3781 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
     3841 gataggcacc ggttgggtta tcgctgggaa caaacggcac atcgatgggg aacggaaaac
     3901 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
     3961 tatgccccca gtcagccatt aactcagagg tgaaggcttc aggcacttgc agcgggtggt
     4021 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
     4081 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgt ggcgcaagtc
     4141 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
     4201 aatcggaacc attcatggtg atggtagttt gtgtaattgt tggggagttt agtaaaattt
     4261 ttactaaaaa gtggcaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
     4321 cactgt
//
LOCUS       DAQSWQ010000195         4309 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.197, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000195 DAQSWQ010000000
VERSION     DAQSWQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.197"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(108..1472)
                     /locus_tag="R8G18_004159"
                     /pseudo
     CDS             complement(108..1472)
                     /locus_tag="R8G18_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1537..1656)
                     /locus_tag="R8G18_004160"
     CDS             complement(1537..1656)
                     /locus_tag="R8G18_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEE9797711.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1811..2029)
                     /locus_tag="R8G18_004161"
     CDS             complement(1811..2029)
                     /locus_tag="R8G18_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEE9797712.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2383..3861)
                     /locus_tag="R8G18_004162"
     CDS             complement(2383..3861)
                     /locus_tag="R8G18_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HEE9797713.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1220 a          944 c          759 g         1386 t
ORIGIN      
        1 cctaagacat caatcatctg ctctccaatg actagtctaa aaactagtat taagactatc
       61 acttaaataa gtgatactgg ttgtctggag attcaggggg ccagtctagc aactaaagca
      121 ctgcccgtct cctggttaac cccatgtgct ataccgccgg tatagacaat cagcgagcct
      181 ccggcgctaa tattgctgcc agttgccgta ccatctttct caacaacctg ccggctcccg
      241 gtggaaatat ttgtcgtgtc agctttcccg ccgctcttga tattttgtgt tccgctgttg
      301 atattggtac ccgtggcaat accatgatta ttaatattct gtgtaccacc attgattatg
      361 gtattggtcg cgtttctggt aacatccatt accccgccgt tatctattcg ggtcgcatca
      421 gctttagcat tcgttaaaac tgacattgtt cctttatctt taataatcgt cttatttgcc
      481 gaaccatatg cgtttatgtc taaatgacca ccgttttcca gcaacacatt gtctgccacg
      541 tgattgtgga tggagaatgc accttcacta ttcgtaccgc tcaccgtcgt accgttagtg
      601 tttgttttta aaattgcacc atcgtgctgg gtaacatttg ttgccgtacc accactaaca
      661 tcaagcacgc caccggaata aacttcaata acatccgagg agctggtgta atcaacaatt
      721 tgcgtaccac cagaatagat ctgagtattt tttgccgttg acttattatt cagagactga
      781 gttccacctt caatcgtcgt gtccagcgca tggctttcat atactctttg ctcaccgcca
      841 tttttaatgg tggttgttcc tactgtgctc tgttcaacat actgtcgtcc accatttatg
      901 attgtgttcg ttgccatact tccttgcgtg atgtcctgat aaccggactt atttatcgtt
      961 gtaccatcaa catgcccctg agttgttact gtctgccctc caccatcaat gatggttcca
     1021 ttcgcagtcc ccccacttat gtgtgaatta ccacccttaa ttgtcgttcc attgctgata
     1081 ccacctgtat aaacgtcctg attgccactg tcgattatcg taccggtgga aatgcccccg
     1141 tcatgaattg tctgtcttcc tccgttaatg gttgtattat tagactgccc catataattg
     1201 ttattccttc caacatcttg atatccacca gattcaataa gacttccatt agatactccg
     1261 ccataaacac tctgctggcc atggttgata atatgagtgt tatttgttgt acctcgttca
     1321 tctactcgtt ggttgccatc tacagtctca tcgtttacca caccaataac attagtagta
     1381 aaggccgcca tcacgggcgg ggcatatatc aaggcagata tcaataagga aagtactgag
     1441 cggcgacaat aatggggact ggtcctgttc ataaatttca tcctctgaaa agtgaatact
     1501 gagtagcgtt taagcgactt tagctctgct gcaacatcag cccgcaggca ccagaccggg
     1561 gggttcatcc tgaagagaca gcgcaagtgt attgtgttca ccgctcatca aagacatcat
     1621 gatgaaatga tgatgttcca cataagaaag aggcattttt taaacacagt gcgctgaagt
     1681 gtgattggat aaaaaagtca atctattcag gaaatacggg cgtattcttt tctttcgaca
     1741 atcaggccgt cggcaaaata aaatgattta cataatcgtt tctgatgaat atcttctgct
     1801 cacataaaaa tcacacaata actttgagat cgcagattct tttactttta cagcattcgt
     1861 ccccccattg ttgggcaaat atagattggg ccagagcacg aaagttaata ccacgtttgc
     1921 acagctcctc caacagcacg acaagatgcc acatactgcg ccccagtcgg tgcggtttac
     1981 agactacccg tgcgtccacc gccgataatg tcctgagcag ttttttcagt ccggacctcc
     2041 tgccacggtt ttacgttgaa gaggtaaccc tgagcacgca gttcttcagt caggcgtggt
     2101 gcaccgtaac gctgttattg ctgggtaaga tcaaaaaact ttcaggcagc taaggaaagt
     2161 tgaaccagac attagatgaa atatttcaac caattacagc accaatttaa actcagtaaa
     2221 tcacagcaca gatcaagggg ttacgtgaaa gcgtagcccc tttttctttg gtagtggcag
     2281 caaaatggtt gtagtgtaaa aaataatccc gtttaatcaa tcaataatac atattgtttc
     2341 aatctacgtt attatctctt tgtaaaaata gccatttatt aatcattgaa aactgctttt
     2401 agaacttgat acaacgggac tagtcacaac aggactattc tcaacgggat catcctcaga
     2461 ggaactatca tcaaagtcat catccataaa taaaatatca tcgaatggtg ccacgcccgt
     2521 gatgagtttt attttattat tacgatcagt caagactcca cttaaaccgt tttcgctcac
     2581 aggttttaat gatttttcat tactcttgtt gtaagcaggc gcattaaaaa tacacggagt
     2641 atcaagatca aacaatgacg ttccccagtt cacatattga atatcatagt tactgaagtt
     2701 ctgtccagaa aagaagcatc ccttaaaatc caatccacct aaattatata aaccatcctc
     2761 ttctttttgg agagtaatgt taattctggc tatctcccgg acaaaacttc cttttttgta
     2821 tttgaatacc gtttcaagat tttcaccaga cagcttgact tctgggaata atttaaaatt
     2881 aaaccctaca tcattattat tcaaagtaga tgaacgaaag atggaaaaag cttgtagtgc
     2941 tgaattatag ctttcgattt tatcttgagg ctgcgctctt ggtaaaaact tatagcagca
     3001 ctcataaaaa ttgaataaac gttctgaaca attcccattt ttatcaatta ataccccccg
     3061 gaattctttc acaaaggcat ccgttatttt ttgaatatct atggtgtgtt cctttgactc
     3121 tgacaaagat ggagtctgtt gaagtgcatt gctcatctgg ttaaggaaca tctgcatttc
     3181 tttaaattta tctccatagt ggcctttaac ttgctgagag tttttagtta caggtgaaac
     3241 cttatctatt tggttgtcat ataaattatg tggttccata cagacatttg gttgtgtttc
     3301 taaaataaca ctacgtgtac tacctggctt aaatgaatca acatgaaaat cactttttct
     3361 tatattatcg aatagggtct gttcatttct cttagcgcat tcaataaact gatcaacatt
     3421 atttttattc gatgacaggt cagtttcaga aaccgcattt tctttgttct ccagctttag
     3481 tttaatgaga agatcttccc agacctgctt gcttaaacat actacgtcag gctcaccatc
     3541 agtaactaac tcttcattat tatcaaagta tacgttgaat acttttaagt tattttcatc
     3601 aacatcatat ttaatacgtt ttaattgctc tccagatccc ataatgacaa aggcgttgcc
     3661 ttcatgatat atacattcag acatcatttt ttgtaaagtt tcaagagcac cgcgatacgt
     3721 ccctgaggca gcttccttac aaattaaatt tataatacta taagctagta gctccatgga
     3781 ttcagaagat tttctttcat tacaatttcc actatctata aatgagctag tgttaaattc
     3841 gttgctttta ctaactaaca ttgtctattc ctcagttaat gtctacatgg ctatttttaa
     3901 ttttattatt gtttgtcact ataaaaaatc gctcatttga gacaattgct ggcattaaca
     3961 gcttcatatg ctatacatgg tacttttaat taaattagca caagaatgtt aaatttaata
     4021 aacaaaaagt tatttcgctg tatgataaaa accacccgtt ataatttatt agtgacattc
     4081 gtttttcgag tgttagaaat ttatatctaa atagcgttga ttaatgagca aagccagact
     4141 cctgtaacgc tcacaagact catctacctg cgacgggtat tctggtcagt agtagatgtt
     4201 taaggcgtgg cagagacatt tcatccttac tctacggcat tggcccgcat acaaatcgtg
     4261 gttgtgacac tcacttatca tcagtgagcg aacagagaat agttcagtg
//
LOCUS       DAQSWQ010000196         4309 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.198, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000196 DAQSWQ010000000
VERSION     DAQSWQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.198"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="R8G18_004163"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="R8G18_004163"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HEE9797714.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            2269..2976
                     /locus_tag="R8G18_004164"
     CDS             2269..2976
                     /locus_tag="R8G18_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEE9797715.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="R8G18_004165"
     tRNA            3082..3157
                     /locus_tag="R8G18_004165"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="R8G18_004166"
     CDS             3355..>4309
                     /locus_tag="R8G18_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEE9797716.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1140 c         1037 g         1018 t
ORIGIN      
        1 gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggcgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgtagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agccttgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAQSWQ010000197         4261 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.199, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000197 DAQSWQ010000000
VERSION     DAQSWQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.199"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..3511)
                     /locus_tag="R8G18_004167"
     CDS             complement(<1..3511)
                     /locus_tag="R8G18_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEE9797717.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
     gene            complement(3963..>4261)
                     /locus_tag="R8G18_004168"
     CDS             complement(3963..>4261)
                     /locus_tag="R8G18_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEE9797718.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1098 a         1095 c          878 g         1190 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta caaagaaaaa taatcagtta aagcagtccg ggcaatcagc ccggactgct
     3961 tactatttta atggattgtt aatctttgca tattcaaacg ggctgatacc cttatctctg
     4021 ttcgcatcca gaaaatctgc ccaccactgt agcatcagtc gacgttcttc cagatgttct
     4081 gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc
     4141 acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat
     4201 ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc tttatttaca
     4261 g
//
LOCUS       DAQSWQ010000198         4223 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.200, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000198 DAQSWQ010000000
VERSION     DAQSWQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.200"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="R8G18_004169"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="R8G18_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEE9797719.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="R8G18_004170"
     tRNA            complement(330..405)
                     /locus_tag="R8G18_004170"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="R8G18_004171"
     tRNA            complement(412..486)
                     /locus_tag="R8G18_004171"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="R8G18_004172"
     tRNA            complement(603..687)
                     /locus_tag="R8G18_004172"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="R8G18_004173"
     tRNA            complement(696..771)
                     /locus_tag="R8G18_004173"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="R8G18_004174"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="R8G18_004174"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEE9797720.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="R8G18_004175"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="R8G18_004175"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HEE9797721.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="R8G18_004176"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="R8G18_004176"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEE9797722.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1088 a         1052 c          944 g         1139 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttc
//
LOCUS       DAQSWQ010000199         4179 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.201, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000199 DAQSWQ010000000
VERSION     DAQSWQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.201"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="R8G18_004177"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="R8G18_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HEE9797723.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="R8G18_004178"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="R8G18_004178"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HEE9797724.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="R8G18_004179"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="R8G18_004179"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HEE9797725.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         1030 a         1062 c          965 g         1122 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatggcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgaga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgttcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcg
//
LOCUS       DAQSWQ010000200         4087 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.202, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000200 DAQSWQ010000000
VERSION     DAQSWQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.202"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..877
                     /gene="adeQ"
                     /locus_tag="R8G18_004180"
                     /gene_synonym="yicO"
     CDS             <1..877
                     /gene="adeQ"
                     /locus_tag="R8G18_004180"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HEE9797726.1"
                     /translation="GLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIIT
                     VLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELA
                     GIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSS
                     VTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMT
                     SSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCV
                     VVVASLFALKIILVD"
     gene            930..1382
                     /locus_tag="R8G18_004181"
     CDS             930..1382
                     /locus_tag="R8G18_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HEE9797727.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1593..2773
                     /gene="nepI"
                     /locus_tag="R8G18_004182"
                     /pseudo
     CDS             1593..2773
                     /gene="nepI"
                     /locus_tag="R8G18_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2814..3107)
                     /locus_tag="R8G18_004183"
     CDS             complement(2814..3107)
                     /locus_tag="R8G18_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797728.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3329..>4087
                     /gene="nlpA"
                     /locus_tag="R8G18_004184"
     CDS             3329..>4087
                     /gene="nlpA"
                     /locus_tag="R8G18_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HEE9797729.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          900 a          906 c         1077 g         1204 t
ORIGIN      
        1 tggattattt atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga
       61 cacgctggtg atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt
      121 ttttattata accgtgttgt catcacgtca ttttcatgcc gcggtgctgg tttctattgt
      181 ggtgacgtct tgctgtggat tatttttcgg tgatgttcat tttagcggcg tctattccat
      241 tccgcctgat attagcggcg tcattggtga agtagatttg agcggcgcgt taacacttga
      301 actcgccggt atcattttct cctttatgct gatcaaccta tttgattcat caggaacatt
      361 aattggtgta actgataaag cgggcttaat agatggtaac ggtaaattcc ccaatatgaa
      421 taaggcgctg tatgttgata gcgtcagttc ggtggcgggt gcgtttatcg gcacctcgtc
      481 tgttactgcc tatattgaaa gtacttctgg tgtggcagtc ggtggccgca cggggctgac
      541 tgcggttgtg gttggcgtta tgttcctgtt ggttatgttc ttctccccgc tggtggcgat
      601 ggttcctcct tacgcaaccg ccggagcgtt aatctttgtt ggcgtgctga tgacttcgag
      661 cctggcgcgc gttaactggg atgattttac cgaatcggtg cctgcgttta ttaccacggt
      721 gatgatgccc tttactttct cgatcaccga agggattgca ctcggcttta tgtcgtactg
      781 catcatgaaa gtgtgcaccg ggcgctggcg cgatctgaac ctgtgtgtgg tggtggtcgc
      841 atctctgttt gcactgaaga ttattctggt ggattagtaa ctggcgaggg tttccagata
      901 tcatgagttc tgataacgca ggagaactca tgatctggat aatgctcgcc acgctggcgg
      961 tagtgtttgt ggttggtttt cgggtgctga catccggggc cagaaaagcg attcgccgtc
     1021 tcagtgatcg gctgaatatc gatgtcgtac ccgtggagtc gatggtcgat caaatgggaa
     1081 agtcagccgg tgacgaattt ttacgttatt tgcatcgccc ggatgagtcg cacctgcaaa
     1141 acgccgcgca ggtgttgctc atctggcaaa ttgtcattgt cgatggtagc gaacagaacc
     1201 tgctgcaatg gcatcggatt ttacaaaaag cccgcctttc cgcgccgatt accgatgctc
     1261 aggtcaggct ggcgctgggt tttctgcgcg aaaccgaacc tgaaatgcag gatattaatg
     1321 cttttcagat gcgctataac gcgttctttc agcctgccga gggcgttcac tggctgcatt
     1381 aaaaccgctt cgtcttatca gcctacagac cgcgcctgac ctgtcgggtg gataagacgt
     1441 tcacgccgca tccggtaatc tttgccctgt tccgtttcga tcttaactcc agctcaagca
     1501 acaccgtaaa agttgcaaaa acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg
     1561 cttttcccct gaaacatgcc acgggtaaca ccatgagtga atttattgcc gaaaaccgcg
     1621 gcgcggatgc catcacccga ccgaactggt cagccgtttt ctcggtggcg ttttgtgtcg
     1681 cctgtctgat tatcgttgag tttttgcccg tcagtttgtt gacgccaatg gcccaggatt
     1741 taggcatttc ggaaggggtt gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt
     1801 ttgccagttt gtttattacc cagacaattc aggctactga ccgccgcaac gttgttattt
     1861 tgtttgccgt tttgctgacg ctctcctgct tgctggtttc ctttgctaac tcattcagtt
     1921 tgcttttaat cggtcgtgcc tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg
     1981 cgtcgctgac catgcgtctg gtgccgccgc gtacggtgcc gaaggcgctg tcggtgatct
     2041 tcggcgcggt ttctattgcg ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc
     2101 ttatcggttg gcgcaatgtc tttaatgcgg cggcggtgat gggcgtgctg tgtattttct
     2161 ggattatcaa atcattgcct tcactgccag gcgaaccctc gcatcagaaa caaaatactt
     2221 tccgcttatt acaacgtccg ggtgtgatgg cagggatgat cgccatcttc atgtctttcg
     2281 ccgggcagtt tgctttcttc acatatattc gcccggtgta tatgaacctg gcgggattca
     2341 gcgtggatgg cttaacgctg gtgctgttga gttttggcat tgccagcttt atcggtacgt
     2401 cgctttcgtc attcattctt aaacgttcgg taaaactggc cttagcaggc gcgccgttaa
     2461 tactggctgt gagtgcgttg gtgctgacgt tgtggggaag cgataaaatc gttgctaccg
     2521 gcgtggcgat tatctggggg ctaacttttg cattggttcc cgtcggctgg tcaacgtgga
     2581 tcacccgctc actggccgat caggcagaaa aagccgggtc tattcaggtg gcggttattc
     2641 agcttgtggc gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt
     2701 gatgttttct ggcacattga tgttgctgac tgcattgttg gttactgcaa aggtgaaaat
     2761 gaagaaatcc tgatatcagg taaagaagcg ttggtcagta gttgttgctg ttcttacata
     2821 tccgggcatt ctaccttacc caacgcttcc caataattgc tctgattatt acgttccatc
     2881 gccacgatgg cctcgcgtat atgttgttta ttgttttcat ctgacaacat taacttttcc
     2941 cacgcttcat ctgataatga cgcctgtggc gtgcaaattt tacgtaaatc ttgtaatacc
     3001 gtggttttct cttttgcgga ctgcagtgag ctaaaaggcg actcggcgta tacgatgcct
     3061 ggcatagcga aaaaagtgaa aataagcagt agtgtcgttg gtttcatgga ttcaaccttt
     3121 tacaaatgtg tatcgaatga agcataagtg tagtttgctt tcatcgctct catcatgctg
     3181 tacgaaacgc gcttgcgtgg tcagtaagaa gtgccagact ttatattcca cttttattcc
     3241 tttttattct tatcgatagc gtttcgtttt ttaaaccgca gcgaccttac cgctatagtc
     3301 aggtaatcat taataaaagg ataaaaaaat gaaactgaca acacatcatc tacggacagg
     3361 ggccgcatta ttgctggccg gaattctgct ggcaggttgc gaccagagta gcagcgatgc
     3421 aaaacacatt aaagttggcg ttataaatgg cgcagaacaa gatgtcgcgg aagtcgctaa
     3481 aaaggtggca aaagagaaat atggcctcga tgttgaactg gtaggattta gcggttcgtt
     3541 gctaccgaac gatgcgacaa atcatggcga acttgatgcg aatgtcttcc agcatcgacc
     3601 ctttcttgaa caagataatc aggcgcatgg ttataaactg gtagcggtgg gaaatacgtt
     3661 tgtcttccct atggcgggtt attccaaaaa aatcaaaacc gtggcgcaaa taaaagaagg
     3721 cgcgacagtg gcgatcccca acgacccgac caaccttggt cgcgcacttt tactgttgca
     3781 aaaagagaag ctaattactc tgaaagaggg aaaagggtta ttacctaccg cgctggatat
     3841 aaccgataat cctcgtcatt tgcagattat ggaactcgag ggggcgcagc tcccgcgcgt
     3901 actggatgat cccaaagttg atgtagcgat tatcagcacc acttacattc agcagacagg
     3961 gctttctccg gtgcacgaca gcgtatttat tgaagataag aattcgccgt atgtgaatat
     4021 tttggtggca cgggaagata ataagaatgc agaaaacgtg aaggaatttc tgcaatctta
     4081 tcaatca
//
LOCUS       DAQSWQ010000201         4077 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.203, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000201 DAQSWQ010000000
VERSION     DAQSWQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.203"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..786
                     /locus_tag="R8G18_004185"
     CDS             <1..786
                     /locus_tag="R8G18_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HEE9797730.1"
                     /translation="IAKIRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            799..1758
                     /locus_tag="R8G18_004186"
     CDS             799..1758
                     /locus_tag="R8G18_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HEE9797731.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1796..2695)
                     /gene="nhaR"
                     /locus_tag="R8G18_004187"
     CDS             complement(1796..2695)
                     /gene="nhaR"
                     /locus_tag="R8G18_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEE9797732.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2761..3927)
                     /gene="nhaA"
                     /locus_tag="R8G18_004188"
     CDS             complement(2761..3927)
                     /gene="nhaA"
                     /locus_tag="R8G18_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEE9797733.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          951 c          861 g         1040 t
ORIGIN      
        1 attgccaaaa tacgtcacca aattaactta tcgaattcga cctcattttc caaagatggc
       61 tattcctcca acaatactgg aattactggc atagccggtg aacatgatca gttaaattat
      121 ggtatatatg ttaatcagca acaacaaaat aatgatacct cgcttggtac gaatttaagc
      181 tggagaactc ccatcgccat aatagatggc agctatagtc attctaaaaa cgcctggcaa
      241 agtggtggaa gtattagtag tggattagtt gtctggcccg gtggtattaa tatcactaac
      301 cagttatccg atacatttgc aattctggat gcgcctgggt tagaaggtgc acatattaat
      361 ggacaaaaat acaatcgaac aaacagcaaa ggccaggttg tttacgacct gataatacct
      421 catcgtgaaa accatctggt acttgatata gcaaacagtg aaagtgaaac agaattgcag
      481 ggcaatcgtc aaattattgc accttaccgt ggagcagttt cttatgtgca gtttacaact
      541 gaccaacgta agccctggta tatacaggca ctgcgtcccg atggttcgcc attaaccttt
      601 ggctatgacg tactggatct ccaggaaaat aatattggag tcgttggcca gggtagtcgc
      661 ctttttattc gcgtagatga aattcccact ggaataaaag ttgctctcaa tgatgaacag
      721 aacttattct gtactattac ttttcaacac gttatcgatg aaaacaaaac atatatatgc
      781 cagtaaaagg agtttactat gaagtggctt ttattaatca ccttgagtct atattctttt
      841 attgtgcaaa gtgctccctg tgcattgacc aatgtcgggg aacaacgtgg aacatatata
      901 ctcaagccac tgagtatgaa aggtaatctc accgccgaaa aaattgaaaa caccggaaat
      961 atcattgagt gtgcagactt acaagagacg accaacgcca ttaaagttca ttttttaaca
     1021 cagaacgctt taaggtttaa agtcggaagt cgtcattata ttattaattt cccctttgaa
     1081 tctgacggta aaaaaataga gcttccagga aggtcatctt ataatttaga agaaatgcta
     1141 tctgcgataa atttcaccat tccatactct atcgcatctg tatcagatag ttccaacacc
     1201 gcagatattt ttccaggaac tccttttcct ttgttgttcg aaattgaagt gagttcatgt
     1261 acagggaata atactaattg taatagcgtc cgctttaatt ataatttaaa tactatttta
     1321 caggtaaatt taatgacctg tggttttgaa gatcaatcca tcgacactgg acgatttgta
     1381 ctgtctcgta tcaataatga aaattatcag tttcatcata taagatttga ctgcatccag
     1441 ggcgagcaag gtgaattagt ttttgaaccc agtgatgttg aatattactt tgaaccagtg
     1501 acgatacagg agtcaggaac cagcatatta aaaaatgagt tagaaaacag tgaggacggt
     1561 gcgggtcaag taggctttca actctccaat gatggtacga atgaaatcca gtatggtaaa
     1621 agcaattact actcttttca gcatccccat gaaggaagta atcagattcc tctatttatt
     1681 cgcccgcgta catatggaaa taatgtctca agcgggcaaa taatgagccg agtcaaaatc
     1741 gtggttatgt acaactaatc tatctccccc accttaactt tgggggaggt gccgattaac
     1801 gcgccgctgg actaaaaagc gcagaataat ccgtattgca gattcgctgt accgccgggt
     1861 gctgaatcat ccgctcagca aaaatagcat ggtactcttc catcacattc tcgacgcgac
     1921 caatttctac gacagtttta tcggcataaa agtcatatgc ataaagcgtt ggggcaacga
     1981 agattgcatt gtgcatcgca ccaaaagctt tcatcaacgc ggcatcatca aactcgccga
     2041 ggatttctac gtttaatccc tgggagttaa accagttaag caatttgcgc cctaacattg
     2101 aacgtcgccc aggaatcaaa agtcgccgtt cttccagaca agccgggaac gatttttctg
     2161 gtggtggatt tgtacaccag aaactcacgc cacattcgcc aattctcacg gagaacaggc
     2221 cttcctgctg cgtagagtct atcggacagt cagaaatgat catatccagt ttatgctgac
     2281 ttaattgctc cagcagcatt tcgtgggtgg attcgaagca gcgaagatga atgggctcgc
     2341 cttctaccac tgcggcgtta agtacgctac tgaccaggcg tttggaaagt gcatcagcca
     2401 cgccaacgtc aaacaataaa ttggattctt tgcgatagtt cacaatatcc agcatttcct
     2461 ggcttaaggt gaacatttta tcggcatagc gatagaccag ttctcccagc tcgctgggtt
     2521 cgagaccacg tcccttgcgt ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa
     2581 tctgtccggt aatggtttgt ggagttaaat aaagcgcctc cgctgcgcca accacggaac
     2641 cttctttata gacatgccag aagtaataca agtggttgta attgatatga gacatgctca
     2701 tttctctccc tgataacaat gaaaagggag ccgtttatgg ctccccggta aaccgtcctg
     2761 tcaaactgat ggacgcaaac gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat
     2821 agaaccgacc aggataccga gtttcgccca gttaatcagt tctggatcta cgctaccaaa
     2881 ggccaggctg gcaataaaga tagacatagt aaaaccgata ccgcacagga tccccaccgc
     2941 cataatttgc tgataagtcg ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa
     3001 ccagcagaac agactaatcc ccagcggttt gccaatcagc aagccagcga tgatccccaa
     3061 tggcagaatg gaggtcaagc catccagcgt gacgccttgc agtgaaacgc cagcattagc
     3121 aaatgcaaac agcggtaaaa tcagatacgc cacccacggg tgcaatacat gctccagtcg
     3181 cttcgctgga gaacgcccat gcttctcttt caaaggaata aagaagccga caattacccc
     3241 cgccagggtt gcgtgaaccc ccgatttcaa caccgcagtc cacaacacca cgccaacaag
     3301 aatatagacg cccgtgcggc gtacaccaca cagattcaat accgcgagta ccgcaattgc
     3361 tacagccgcg acgccaagag aggccatcga taagtcatta gtgtagaaca atgcgatgat
     3421 aatgatggcc ccaagatcgt cgataatagc cagagccatc aaaaagatct tcagtgctaa
     3481 cggaacacga cttcccaaca gcgccagcac accaagtgca aaggcaatgt cagtagccgc
     3541 cgggatcgcc cacccttcgc gggtaatcgg atcggcatag ttaaaggcca gatagagtaa
     3601 tgccggaaca atcatcccac caatagcggc gataaccgga aatgcggcct ggcgtaagct
     3661 ggctagcgat ccttgcatca gttcacgttt aacttccaga ccgactaaca ggaaaaatac
     3721 cgccatcagc gcgtcattta tccataacag catgtttttg ttgatttcga gtgaaccaac
     3781 ccggagctga accggcgtct ccagaaagtc gtgataccat ccactggttg cgccgctgtt
     3841 ggccatcatc atcgccagga tagcggcaat gataagaata atgcctcccg aggcatcact
     3901 gctaaagaat cgatgcagat gtttcacttt ttatttctct ttcaggtgaa tagatcgttt
     3961 ttactatttt accccgcaca aatcatcgtt aaaatcagat tatagtgtag gaataactcg
     4021 ttttttacga gcaattaaag gatttgcgcc atcagcggtg agatgagttt ttgacgg
//
LOCUS       DAQSWQ010000202         4061 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.204, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000202 DAQSWQ010000000
VERSION     DAQSWQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.204"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            65..784
                     /locus_tag="R8G18_004189"
     CDS             65..784
                     /locus_tag="R8G18_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
                     /protein_id="HEE9797734.1"
                     /translation="MKFSDRREALKLMTQIESTVTSLHREAEAQFRPELEKIVRGIET
                     GFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQLQTRIAVKTNLSALMEERTET
                     VTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVININSVRKEVMSLTRAYFGELQ
                     ASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSSEDHKRDQQAQEQLTERLQALN
                     ERVPELITDSKALREELETLL"
     gene            781..1686
                     /locus_tag="R8G18_004190"
     CDS             781..1686
                     /locus_tag="R8G18_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEE9797735.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1683..2753
                     /locus_tag="R8G18_004191"
     CDS             1683..2753
                     /locus_tag="R8G18_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEE9797736.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2885..3343
                     /locus_tag="R8G18_004192"
                     /pseudo
     CDS             2885..3343
                     /locus_tag="R8G18_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3469..4008
                     /locus_tag="R8G18_004193"
     CDS             3469..4008
                     /locus_tag="R8G18_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEE9797737.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT         1077 a          900 c         1101 g          983 t
ORIGIN      
        1 gtttagtgtc agtgccccgg gccatcattc atggaaacga gactttgatc cggacagccc
       61 cgagataaaa ttcagtgatc gcagggaagc ccttaaactg atgacgcaaa tcgaatcgac
      121 cgtgaccagc ctgcaccgtg aggctgaagc acagttccgg cctgaactgg aaaaaatcgt
      181 caggggtatc gaaacaggtt ttcgtggtac ggccctgtat gccacagaaa atattgccgg
      241 acgtatcaat gcccgcctgg aggacgaggg cttcactgta aaaatcactt tcccggcagt
      301 cagccagtta cagaccagga tcgcggtaaa aacaaatctg agtgcgctta tggaggaaag
      361 aactgagaca gtcacccgtc gccgtcggca gagtggtttc tggggaaaga tttgtggagc
      421 gtttggcacc agtgactggg gctgggaaaa ctataaggag aatgtgagcc gcagtgtgat
      481 caatatcaac tcggtcagga aggaagtgat gtcactgacc cgggcatatt tcggggagtt
      541 gcaggcatcc attgagcaga atattaacca gcccgtccgc caggagatcg atgatttttt
      601 ctgtacattc agggaaaaag tcgaacagct acgtaacaca ctcattcaga gctctgaaga
      661 tcataaacgc gatcagcagg cacaggagca gcttacggag cgccttcagg cattaaatga
      721 aagggttccc gagctgatta ctgacagtaa ggcgttaagg gaagaactgg agacactgct
      781 gtgacctcac catttattca acaaattgct gataaccggg tatgtcaggt actcagttgc
      841 cttcctgaaa aatttgtggt tgattttgca aacggtattg atgttgcaca ggagcataac
      901 cgcacggccg gggggcgcac gtttttccgg cgtttaaaag aaggtctgac tggcaaaggc
      961 gctgtacgcc agaatgccat caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc
     1021 tggctgacgg agctgacaac gtcgcttgcc accacaaatt acgcgattac ccgggtaaac
     1081 gacagggtca gttcactggt cagtgatact gccaggctgg cgcattattc ggcagacacg
     1141 cgggagcagt tactcaccct ggcagaacag gttcatcaga aactgaatca tcttgaagaa
     1201 aaactccacc gtgttgacca ggttcagcgg gcacagttac atcttgagca gatattctca
     1261 tggtggagcg ccgggcgata cgcgtcattt tcccctgccg gacgttgtta tgtggcgctt
     1321 gaagagcttc gctggggcgc gtttggtgat gtgatacgtc agggtgaaac aggccaggtt
     1381 aaccagttac tggatatcct cagacataaa gcattaacgc aaatggcaca ggagaatggc
     1441 ggtagcgcaa cagtacgtct gaacacactg gactggcttg gtggtcagag ccgggaacag
     1501 gctgataacg aatggcatga agcggttaac tggctggggg actggtgcag tgaagagcgg
     1561 catcctgtga tctggtcaac cacacaggct gcagaacact taccggttcg tatgccccgt
     1621 ctctgctctg cagaacgcct ctctgaaagt atggttgatg aaatatttca gaagggggag
     1681 gcatgagtac agaaatgaaa acggggctgg tgctgtccgg cggcggggcg gtgggcgctt
     1741 atcaggcggg agtggttaag gcactggcag agtgcggcgc acagatcagt atggtctcag
     1801 gagccagtat cggggctctc aatggtgcca ttatcacggc ctctcccgat ctgtcagaag
     1861 ccgccttacg cctggaggcg ctctgggatc atctggggaa taatcaggtt ctgtcggtaa
     1921 acagatcggt ttacttttca ttgctgaaaa aactggttca ggccatgaac ctctgccaga
     1981 tccccggacg tgcgggcgca ctgcttacga cgctttttcg ccatatatcg acaatcaacg
     2041 ggttcgacaa tccgatgatt cagccgctgt tgtcagatga gcccctgaca gcgctgatgg
     2101 atcattatct tgatacagat gctctggccg acgggctacc gctgtacgtg tcgctgtacc
     2161 ccacagaagg aggcatgcag gatattattg actgcattcg tgctgaactg ggtgccggaa
     2221 ccacgaaaaa cgctgttttt cagcatatcc agagtctgcc ccgcgggcag cagaaagaag
     2281 ccttactggc gtcagccgcg ctgcctctgc tgttccgtcc ccgcgaggtt cagggaacaa
     2341 tgtacggtga tggtgggatg ggcggatggc gaaacaggca ggggaatact cccgtgacgc
     2401 cactggtgga tgccggatgc aatatggtga ttgtgacgca tctgagtgac ggttctttat
     2461 gggatcgtcg ggcttatccg gacaccacaa tccttgaaat ccgtccccgg aaaaggttga
     2521 aacaaatcgg ggacgaaggc aaaagtggcg gtctgctcag ttttacatcg gcacataccg
     2581 acgcctggcg tcagcagggc tacgaggaca cgatgctgac gatggagcat atccggaaac
     2641 cgctggcagc acgtcaggca ctgacccggt cagagacggt attgcagaaa agcctggaga
     2701 taactgaagg tgcagattcg gcactgagaa acgcgatggc ccggattaaa taaagacgct
     2761 ccggagaaac gccaccgcca ctgtggtggt gtttttcctg tctggtatta ttgaaaatat
     2821 tttcagatat atattacctg gatgatggtt aagattaatt tatttaattt tggggagaaa
     2881 actcatgaaa ctgttaaaca ttcttttagc tgttgtttta actgtaagtg caaccgtagc
     2941 acaaacagaa tcggcattgt agacgcgtaa cagtattgct ggcactgata tgttcagtat
     3001 cggtaatgtt tctattaact gcacgattaa tccagaagac aacaatttgc taccacacta
     3061 tgtgtggatt tacggtcata cctccaccca gctaaatcag tacgattctg atattatttt
     3121 tatcatcaac gataaaaagt atccggcccc atctgtcgac ggaacacgaa tgaataagaa
     3181 tgcatgggta tactttatcg atgctatcag tgaagccacg caattcgacg ttttagtaaa
     3241 cggtaagaaa gtcgacagct ataccgcaaa tatcaaaaat gtgaaaaaga ctttgggtaa
     3301 caaattctac gacagctgct ggaacacctg gttccaggaa taattgataa cgtggtgtaa
     3361 aacacctcag tttaatttaa tacaatgagt gaaaatgaat attataaaaa tattttttgt
     3421 ttcagatgtc atatatttta ttaactgaat gattaacagg agaatcagat gaactggcat
     3481 tatgaaaaaa atggtgtacg ttatgataac gtaacagagg aagatattat cgaacgtatc
     3541 cggcgtggtg aactgaatgc atcaacactg gtgtggcaac agggcatgct ggaatggcag
     3601 ccagtttcag ccacatcact ggcggaaata ctgaaacaaa gtgaagttcc cccagccctt
     3661 ccgggtcacc gcattcccgg aggcgttgtc tggacgctgg catttgcccc tttgattggg
     3721 tacatacttg agatgtggac tgcagggctg aacggtatgt catttgaacg ggcttatgat
     3781 gcagtcagcg aaggccagta ctggtttatc tctctgattc tgaatattgc tctgggatat
     3841 ctggatgaac gaaaactccg taaatctggt gttgatactg caacatttgg ctggctggca
     3901 tggttgatcc cggtttatct gtggcgtcgg gcaaaaatcc tcggacaaaa acctgcgtat
     3961 ttctgggtct ggttggttat gctgatactg gtgttattta cggtctgata ttctttatcg
     4021 cagagtttac taaataatca ggttattagt gttcttaaca g
//
LOCUS       DAQSWQ010000203         4045 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.205, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000203 DAQSWQ010000000
VERSION     DAQSWQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.205"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="R8G18_004194"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="R8G18_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HEE9797738.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="R8G18_004195"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="R8G18_004195"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HEE9797739.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="R8G18_004196"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="R8G18_004196"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEE9797740.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="R8G18_004197"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="R8G18_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HEE9797741.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1141 c          971 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caacccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DAQSWQ010000204         4041 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.206, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000204 DAQSWQ010000000
VERSION     DAQSWQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.206"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            924..1640
                     /gene="nanC"
                     /locus_tag="R8G18_004198"
     CDS             924..1640
                     /gene="nanC"
                     /locus_tag="R8G18_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HEE9797742.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1660..2766
                     /gene="nanM"
                     /locus_tag="R8G18_004199"
     CDS             1660..2766
                     /gene="nanM"
                     /locus_tag="R8G18_004199"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HEE9797743.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2831..3811
                     /locus_tag="R8G18_004200"
     CDS             2831..3811
                     /locus_tag="R8G18_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEE9797744.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1219 a          845 c          913 g         1064 t
ORIGIN      
        1 agataagggg gaacgcagga gacgcttttt catctacatt cgtgggtaaa tatggctttg
       61 gtcactgcga cttaaacctt cctcaatcac agctattcgc cgggcgatac ggcaaacaaa
      121 ctaagtgaaa catcatcctg aaaacaccaa catcaacaag cctctctaga tcgacttcag
      181 aagcgaccag ttacaagcca caaatcagga aacatatttc atggtgagaa ttatgatcag
      241 cctgatggcg ggtaaacact tgttttcacc acgctcactg agttagcttg caaagcttcc
      301 cttgtttaga ccctaaataa attttttata aaacaaacag ttaaaagaaa tcctttagtt
      361 tttgaagacc acctcaagtg ttcgtctggc ttcacatggc atcttcctct tagaaaaaga
      421 tcgacatatt ttgtgacacg aattgcaaat ctcgttttgt tgtatggctt gcgtgatttt
      481 tgatctggta taacaggtat aaaggtgcac cgcgatagtc aatgagacag ggcatctcgc
      541 aatctatggc aaacatcact tcagttcttt ctcatcgggt gatgaaaacg cacttcagtc
      601 tgaaaggaat atgaaaatga gatcaacaga cactctattt tatgactctg ggtaaaatgg
      661 attgagtaag tgatatagct tacgaacatt caaatcaatt aaacatcaga agagatttta
      721 tactcaggta tttaatctga ttctctgttt atttaaataa tgtggaaaga gaattttcac
      781 aggagacctt atacaaaaat ataaaataag ctacccgttg ccaaagacac tataagcctg
      841 gcaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
      901 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
      961 tgtgcttttc gtcaccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
     1021 gtagcggaag ccatgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
     1081 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
     1141 ccgcactcaa tgatgttcag gttgaagtta attacgcgat taaacttgat gatcaatgga
     1201 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
     1261 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
     1321 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
     1381 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
     1441 tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg
     1501 cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
     1561 ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
     1621 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg
     1681 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
     1741 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
     1801 gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta
     1861 gctgcattcc ctggtggacc gagagatcaa gcaacctctg catttattga tggcaatcta
     1921 tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta
     1981 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgctcgc gccgatgggc
     2041 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     2101 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     2161 atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag
     2221 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg
     2281 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     2341 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     2401 ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     2461 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     2521 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat
     2581 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     2641 tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc
     2701 ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa
     2761 aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg
     2821 cgaaaattat atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg
     2881 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     2941 tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg
     3001 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     3061 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     3121 gcatattgca cggaaattac tgcccttcat tcctaataat gcagggattc ttatcgttcc
     3181 gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca
     3241 cggagccagc catgatgctt gtcgttgggg aacggatact ccgctgtacc aggatttagt
     3301 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     3361 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     3421 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     3481 tactgacgcg ccatggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     3541 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     3601 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     3661 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     3721 gcgaagcagt catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga
     3781 agcaattttg cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct
     3841 tgttcgttct cagagtagac aacaaaaaac aaataagatc aaggtaatat ctcttcactt
     3901 gcgggctcga acagcaggca attgccaata taataatctg gcacaagggt gaaaaacgcg
     3961 caccgcataa accgttgtta ttgcttacag attagccgga tgccttaata gacatccgcg
     4021 ccttttcgat tatggctcgg a
//
LOCUS       DAQSWQ010000205         4041 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.207, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000205 DAQSWQ010000000
VERSION     DAQSWQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.207"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            376..483
                     /locus_tag="R8G18_004201"
     CDS             376..483
                     /locus_tag="R8G18_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HEE9797745.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="R8G18_004202"
     CDS             complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="R8G18_004202"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HEE9797746.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="R8G18_004203"
     CDS             complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="R8G18_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HEE9797747.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="R8G18_004204"
     CDS             complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="R8G18_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HEE9797748.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1032 a         1011 c         1052 g          946 t
ORIGIN      
        1 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
       61 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
      121 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
      181 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
      241 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
      301 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
      361 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
      421 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
      481 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
      541 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
      601 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
      661 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
      721 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagctaggaa gctgctcggt
      781 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
      841 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
      901 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
      961 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
     1021 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
     1081 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
     1141 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
     1201 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
     1261 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
     1321 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
     1381 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
     1441 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
     1501 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
     1561 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
     1621 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
     1681 accgatgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
     1741 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
     1801 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
     1861 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
     1921 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
     1981 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
     2041 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgtggca
     2101 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
     2161 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
     2221 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
     2281 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaataaacgt aaggtgtcgc
     2341 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
     2401 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
     2461 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
     2521 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
     2581 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
     2641 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
     2701 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
     2761 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
     2821 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
     2881 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
     2941 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
     3001 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
     3061 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
     3121 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
     3181 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
     3241 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
     3301 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
     3361 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
     3421 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
     3481 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
     3541 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
     3601 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
     3661 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
     3721 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
     3781 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
     3841 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
     3901 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
     3961 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
     4021 gattatcagt ttactagcga a
//
LOCUS       DAQSWQ010000206         4023 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.208, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000206 DAQSWQ010000000
VERSION     DAQSWQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.208"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..367)
                     /locus_tag="R8G18_004205"
     CDS             complement(<1..367)
                     /locus_tag="R8G18_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HEE9797749.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSE"
     gene            complement(382..1128)
                     /locus_tag="R8G18_004206"
     CDS             complement(382..1128)
                     /locus_tag="R8G18_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEE9797750.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1135..2334)
                     /locus_tag="R8G18_004207"
     CDS             complement(1135..2334)
                     /locus_tag="R8G18_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HEE9797751.1"
                     /translation="MLFVLILSNRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVMFEHDANTKAT
                     NKDTDKDLKTDLTHPKPFPSGREFRDFVAGVLEGRLSGGTAAEFCNSAVVALQAAGLD
                     VCREYPVPERGDGCGGRIDIVVTDRNGVRCGSELDRNSPRQKSLLKIGAVETGICVLR
                     RSDIARHTEQGILVIGGAVRQKKFDPLSVDLPDWLPETLWHEWVQFRQALRKPIRTEQ
                     GANGAIRELEKFRQQGFTPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSA
                     IPPGFRG"
     gene            complement(2347..2769)
                     /locus_tag="R8G18_004208"
     CDS             complement(2347..2769)
                     /locus_tag="R8G18_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797752.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2766..3008)
                     /locus_tag="R8G18_004209"
     CDS             complement(2766..3008)
                     /locus_tag="R8G18_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9797753.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            3105..3524
                     /locus_tag="R8G18_004210"
     CDS             3105..3524
                     /locus_tag="R8G18_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEE9797754.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3830..3985
                     /locus_tag="R8G18_004211"
     CDS             3830..3985
                     /locus_tag="R8G18_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HEE9797755.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT         1007 a         1081 c          894 g         1041 t
ORIGIN      
        1 cttcgctctt ccggcactcg cgacagatta tattcaggcg cctgtcgtag cggcgtattt
       61 ctccgtctgg taatgaccag ataaggtcag gatcaaccac aaccggtttc ttcaccttta
      121 ccctcgatag ctttttgcgg gcgttttgcc agtctttatg agcctgttca gagggaaata
      181 ccccgtagcc agaattatat acatcaccac ttgcgaccag ttccatgcat aagcgaccga
      241 cagacgcacg actgatacct gtttcatctg acaactgccg aatcgtgacc cgaccgtcct
      301 gatgcacgag ttccacaatt cgcgtcttca gttcttcccg ctgttcggga gtaaaaggtt
      361 tcgccataaa tcctcctgaa actacttaac aaccctcgaa tggctaacat tcggacgcca
      421 gctctcccag ttaaaattca cccagcgccc gccgttcatg accatgcgat ccatcacccg
      481 ctcgccgaga agtgtattca tcgctgcatg gttaatattt gtcagcatcc ccacaccgcg
      541 taacgatgcc gtccggcgat caacaatctg gttcagtacc acctgctcgt ttttcgtatc
      601 ccgctgcatg ccaatttcat ccaggaccag caggtcaaca ccacaaagct cctgtaaaaa
      661 tttttcaccg gatttgccgt tgtcgtagct ctcatgcaac acgctcatga catcggacac
      721 ggtgacgata atcacgctac gccccttcgc catcagccgg ttgccaatcg cgtctgcaag
      781 atgatttttt ccggtgccgg gcttaccgct gaacacaaaa ttcgtacagc ctgtcatcag
      841 ttcgtcagct atggatttcg cctgactcag tgcatgtcgc tgaccgtcgt tctgcgcccg
      901 gtaattcgca aacgaacact tccggtgcaa cggctggatg ccggagcggt tcaggatttt
      961 ttccacccgc aactgacgat tcaggcggtt gatctcctcg caacgtttct ggccttcagc
     1021 aagttgccac tcgcgccact ccgcaaccgt tctgaatggg gcggttacat gtggtggggt
     1081 cagtctgcgg atacgctcca gaacgcctcc tgtcgcaata tttttcatgg tctgttaccc
     1141 cctgaagcct ggcgggatcg cactgtccgg caacgagacg gtgttaacct gtcggagcaa
     1201 cgtctcaggc cgaacacctt tcggcgcgaa cagaccctgg tattcattgg cgatgctgtg
     1261 tcgaatcacc tgctcaggtg taaaaccctg ctgacggaat ttttccagtt cccgtatcgc
     1321 cccgttagcg ccctgctccg ttcgaatcgg ttttcgcaat gcctgcctga actggaccca
     1381 ctcatgccag agtgtttctg gcaaccagtc gggcagatca actgacaacg ggtcaaattt
     1441 tttctggcga acagccccac cgataaccag aattccctgc tcggtgtgcc ttgcgatatc
     1501 actgcgccgc aagacacata tcccggtttc aacagcaccg attttgagca gtgatttctg
     1561 tcgcggagaa tttcggtcaa gctcgctccc acaccggaca ccgttcctgt cagtcacgac
     1621 gatatcaatc cgccctccgc aaccgtcacc acgctctggc accggatact cacgacagac
     1681 atccaggcca gcagcctgca acgcaaccac cgcagaatta caaaattccg ctgcagtacc
     1741 gccagataat ctcccctcaa gcactccagc cacaaaatcc cgaaactccc ttccggaagg
     1801 gaagggtttg ggatgggtta gatctgtttt tagatcttta tctgtatctt tattagttgc
     1861 ctttgtgttg gcatcatgtt cgaacatcac tccaacatct gtttgaacac ctgttaaatt
     1921 tctctcttgt tttgtttgaa catctgcttc ctttctgctt cttctggcct gaacagatgc
     1981 ttttcctgcg gctgattttt tggttaattt ttctctgaca gatgccagat cttcctcaat
     2041 ccgaagatgc atccattcgt cgccgttatc acaaaaaaac tcccgcaagg atggttcaac
     2101 atcaacccat cgctcgttag tcagacgggc aatttttgcc aacctgtttt tgggtattgg
     2161 ctttcctgtt tgccagtaat tgaacatcag caacaaatac gcgccgtgct cctctgctga
     2221 caaatgcatg gtgtcagcca ggtaatcagc tatgtacagt tgcatgtatg gtaatgcggc
     2281 cataattgcc ccgtatgatg ctgcccggtt gcttagaata agcacaaaca gcatggaaac
     2341 ttttgcttaa tgaacaatga cagaatcgtc ggaagacccg ccgccgctga aatgcgcttt
     2401 ccggtaaacg gcctggactg catcatcatg cgcatcaatt gccgtactca acgcttcctg
     2461 cgccgccagt aatgcacggc gttccagggt atcgaagatg cagagtcggt gacgcagctc
     2521 gcgcggaaga attgccagaa ccgcagggat cagtttctga attttttccc tttgcgcttt
     2581 cgtttcacct ttcaaccaac ggtgatagat attctgctga ttgttccagt ccttgcctgg
     2641 tacaaggggc aattcgccgc ccccctggcg cagatattct tcagtaattg cgttagcgac
     2701 ccacgcctgc cctttttcgg ctgccagggc taacaacact gattcgatgt gctcatgcct
     2761 gattttcatg aatcaaccgc tcctatgctg ttttcgctat gcttaccgtc tggggggaat
     2821 acatcgtcaa gtccacaatg agcgccaagc cgattaaggg tagaaacaat ttttctgcac
     2881 tcctctagtc caggggtacg aaaatttgct tcgtaatttg ccagtcggct ttgtatccac
     2941 cctaactgaa cagcaagttg tctttgagac agcccaagct gttttcgata tgttgaaatt
     3001 ttgttcattg aaaacctccg atgacaattt taaacacacc ttgtgttata tagccaagct
     3061 gttttgtgtt ttatgtaaat cacgattcgt gatacaagga tgcaatggaa aaagaaaacg
     3121 aaaaaattgc cgctagtagg ctcaatgaca aaattgcaat gcgtcttaaa gagcgcaggc
     3181 agaagcttgg tttatctcaa ggaaaacttg ctgaaatctg cggatggacg caatcgcgta
     3241 taggtaacta tgaggcgggc agcagaaatg ttggagtaca tgacgctgtc gtattgggaa
     3301 aggcacttgg catatctcct cctgagctcc tctttggaga acaggaatct tctgaattgt
     3361 ggttaaatga atcccaacga aaacttcttg agttgtttaa ccagctaccg ggctcagaac
     3421 aacaacgaat gattgagcta tttgaagtcc ggctaaaaga aatcgatgag tatgtagaaa
     3481 aatatttgag aggcaggatt aaagataatc ccccaccgga ataatgatct tgctatcaca
     3541 gtaatatgcc aatcagcccg ctatcagcgg gcttttttgt agcatcatca tatgacactc
     3601 accataaaac acattgcgtg ttgacacaag aaaacaaatt gtgtttaata agcatatcca
     3661 aacaacgccc caccagagaa cggcaggaca atacctcgag ttatccagtc actgaacagg
     3721 gctaagtagc cagcctgagg catacgaaca tgacggcagt tgttgattga tacaaagcgc
     3781 agtagataaa acgttccgcc acccggcgtt aaggggaaat ggggtcaaca tgaatactat
     3841 cgatcttggc aacaacgaat ctctggtatg tggcgtgttt cccaatcaag acggcacatt
     3901 caccgccatg acgtatacca aaagcaaaac gtttaaaacc gaagctggcg cacgtcgttg
     3961 gttgggaaga cattcaggtg agtaaaatga acgagacaga attaaaacac gttatcgctc
     4021 tac
//
LOCUS       DAQSWQ010000207         3959 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.209, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000207 DAQSWQ010000000
VERSION     DAQSWQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.209"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..242)
                     /locus_tag="R8G18_004212"
     CDS             complement(<1..242)
                     /locus_tag="R8G18_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEE9797756.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="R8G18_004213"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="R8G18_004213"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEE9797757.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="R8G18_004214"
     CDS             2255..3904
                     /locus_tag="R8G18_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HEE9797758.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAQSWQ010000208         3945 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.210, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000208 DAQSWQ010000000
VERSION     DAQSWQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.210"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            30..968
                     /locus_tag="R8G18_004215"
     CDS             30..968
                     /locus_tag="R8G18_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HEE9797759.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1024..2313
                     /locus_tag="R8G18_004216"
                     /pseudo
     CDS             1024..2313
                     /locus_tag="R8G18_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2310..2603
                     /locus_tag="R8G18_004217"
     CDS             2310..2603
                     /locus_tag="R8G18_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEE9797760.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2660..3945
                     /gene="fadK"
                     /locus_tag="R8G18_004218"
                     /pseudo
     CDS             2660..3945
                     /gene="fadK"
                     /locus_tag="R8G18_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          930 a         1002 c         1087 g          926 t
ORIGIN      
        1 cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta
       61 gcgataatcc tgaacgttat gctgaactgt ttggcggcgc gcagcaatgg ggccaacagg
      121 tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat
      181 gtatttatgt tcttgagcaa aatgatgcgc tgcaacgtac tgaaaattac gccgaaagca
      241 ttgctgccct gctgaaagat aacccccccg ctatgttgct gctggccgcg acgaaacgtg
      301 gtaaagcgct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca
      361 cggcagtgga tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag
      421 cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc
      481 aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg
      541 ccccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agcggcaagc agcgtggacc
      601 tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcagcgcag gatgacctga
      661 aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg
      721 ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga
      781 aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca
      841 acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg
      901 ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga
      961 gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa
     1021 cgcatgtcgg atgacaaatt tgatgccatt gtggtcggtg cgggcgttgc tggtagcgtt
     1081 gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg cggcgacagt
     1141 gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt
     1201 ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc
     1261 ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcagccaga tgttccgcaa
     1321 cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag
     1381 caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg tgaaggaaac
     1441 aaggtaactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt gattctggct
     1501 gatggcgtta actcgatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat
     1561 cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc
     1621 tttaatatta cgggcgagga aggtgccgcc tagctgtttg ccggttcccc ttctgacggc
     1681 ctgatgggtg gcggattcct ctataccaac aacgattccg tatcgttggg gctggtttgt
     1741 ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa
     1801 caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat
     1861 atggtgccgg aaggcggtct ggcgatggtg ccgcaactgg ttaacgaggg cgtgatgatc
     1921 gttggtgacg ccgcaggctt ctgtctgaat ttgggtttta cggtccgcgg catggattta
     1981 gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat
     2041 ttctccgcca gcagtctggc gcaatacaag cgtgagctgg aacaaagctg cgtcatgcgt
     2101 gatatgcagc attttcgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa
     2161 tacccacgaa tggtagccga catcatgaac gagatgttca ccatcgacgg caaaccaaac
     2221 cagccggtac gaaaaatgat catgggacat gcgaagaaaa ttgggctgat caacttgctg
     2281 aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca
     2341 tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg
     2401 caaatcccga catcaatgaa ttccgtaaat taatgaaagc ctgccctgcc ggactttata
     2461 agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag tgcggcacct
     2521 gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca ggcaccttcg
     2581 gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt
     2641 tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc
     2701 gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc agcagaccgc
     2761 tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt acacctatag
     2821 cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc
     2881 aggcgatcgc atcgcatttc aactgccagg ctggtgtgaa tttaccgtta tctatcttgc
     2941 ctgcctgaaa accggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact
     3001 ggtgtgggtg ctcaataagt gtcaggcaaa aatattcttt gcaccgacgt tgtttaaaca
     3061 aacgcgtccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat
     3121 tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc agattatcgc
     3181 cgacaatatc ccactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct
     3241 gtttacctcc ggaaccgagg gtctgccaaa gggcgtaatg ctaacgcata acaatattct
     3301 cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg tctttatgat
     3361 gcctgcgcca cttggtcacg caacgggatt tctgcatggc gtaacagcac catttttaat
     3421 tggtgctcgc agcgtgttgt tagatatttt cactcctgat gcatgtctcg cgctgcttga
     3481 gcagcagcgt tgcacctgta tgctcggcgc aacgccgttt gtctatgatc ttttgaattt
     3541 actagagaaa caacccgcag acctttcagc gctgcgcttc tttctttgcg gcggtaccac
     3601 aatccccaaa aaagtggcgc gcgaatgcca gcagcgctgg cattaaatta ttaagtgttt
     3661 atggttccac agaaagttcg ccgcatgtgg tggtgaatct caatgatcct ttgtcgcgct
     3721 ttatgcacac cgatggttac gctgccgcag gtgtagagat taaagtggtc gatgacgcac
     3781 gcaagacctt accgccaggt tgcgaaggtg aagaagcctc gcgtggcccc aatgtgttta
     3841 tggggtattt tgatgaacct gaattaaccg cccgtgcgct ggatgaagaa ggctggtatt
     3901 acagcggcga tctctgccgt atggatgagg ctggctatat aaaaa
//
LOCUS       DAQSWQ010000209         3936 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.211, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000209 DAQSWQ010000000
VERSION     DAQSWQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.211"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..585
                     /gene="fimZ"
                     /locus_tag="R8G18_004219"
     CDS             <1..585
                     /gene="fimZ"
                     /locus_tag="R8G18_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HEE9797761.1"
                     /translation="MSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDLP
                     GTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQ
                     MILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLLL
                     SNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="R8G18_004220"
     CDS             complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="R8G18_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HEE9797762.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="R8G18_004221"
     CDS             complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="R8G18_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HEE9797763.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2134..3936)
                     /gene="fimD"
                     /locus_tag="R8G18_004222"
                     /pseudo
     CDS             complement(2134..3936)
                     /gene="fimD"
                     /locus_tag="R8G18_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
BASE COUNT         1094 a          971 c          861 g         1010 t
ORIGIN      
        1 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
       61 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
      121 atagacttgc ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
      181 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagagcgata
      241 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatccc
      301 gttcagatga tcctctccgg atacacgttt tttcctagcg aaacgctcaa ctatataaaa
      361 agcaataaat gtagtacgaa tagttcaacg gtcactgtgc tatctaatcg tgaagtgacc
      421 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttattactt
      481 agcaataaaa cagttagtgc gcataaatct aatatttatg gcaaactagg tttgcattca
      541 attgtagagc ttatcgacta cgccaaatta tacgaattaa tataatatta attataattg
      601 atcataaata tcgcatccgc tttctccaca cctggctgaa caccgctggc taacgctcga
      661 taattggcaa aaaagtttag tgtgacattg ccatttgcat ctacttcctc agtcgggctc
      721 gcctccccca gtgcgagccg ggagcgatcg ctattacgta attcgatggc gacggtttgt
      781 gccattgcgg gatcatccag agccagcagg ttggtatcgg atgccggcgt tcccgtaaat
      841 aaaatcgcaa ctgaacccgg aggacatccc tccagccgca ggctaaaagg gacgagtgcc
      901 gtggtatcgc cagcgttcag taattgtgtc gtaggccatc tgcctaaatc taccgtctta
      961 tcaatatccg cagtgtttac ggtacaggag aaatcaacaa cgttaccgtg caaattgata
     1021 ttaatcgttc ccaaagggtc agctgcccat ccactggaac tccacagtaa cccgcagaaa
     1081 cagctaaaga gtactcttct cattctccgt acctcattga taatcgacgc gtaaataccc
     1141 cagcgcgcta aacggccctt cggtcggttt ttgaccggta atactgatag gccaggcgcg
     1201 aagtgtgaca ttggctgccg cagctgcatc cagacggaaa ggaataacgc tattgagatc
     1261 gttaggcgtg atcggcgtat cgttctgatc ggcgacaata aaacctaaat cctgattgtc
     1321 cgacaccatc gcctgaccag aaacggcact ggcttccaga cgcattgtta aataagcctg
     1381 cgcagcaaca ttcgtacatt tgatcgcaat gctcttggtt tgcggcatga cgccagctgg
     1441 tcgattcccc ggccctgccg cactaaataa cgatgcgccg atatcaccaa aatcaaattc
     1501 aacaatctgc ccggcattta attcgcagtt ttgcggcact tcaacccggc caccaaaact
     1561 aatggtataa acaggtgtaa ccaacggatc gtaaggcgtt gatgtcacat acacggtaaa
     1621 catcacctgt ctggggatct ccaccatgtt aataaaggga cgaataacct ttaaacgaaa
     1681 tatcaaacga gaatccatca ccccgaacgg aaggttttgc gaaacgttag gatctgttcc
     1741 catccgaata taattttgcg gggggaactg aatatccatc acactatcaa ccagactcat
     1801 cgcacctaat agataatcat ttaattgcat atatttataa ttatcgatag tttcctgaac
     1861 aataaagtta gtgacataac tacggtatgt ataattcacc agcgtgccgg gtggacaaat
     1921 tgctgagaca cctacccagc cggatttttc cggtaaaaca acaatctgcc ctggctggtt
     1981 attggtgcta ttaaagacat ttgagagatc atagtgttca tcttctgcta cgccagtact
     2041 atttgcacaa acagtagccc aggatatgtt tgccagacac aaacatgcca ttgccaataa
     2101 taatctgcag attatcttca ttgataattt cctttatgag ccaggatgtg tgcaaactgc
     2161 cgaaataaca gtgacggctt gctgtaagct ttgctttggc aatacatagt gcgcaatgca
     2221 gcgtgagttc gcgccgtctc cccactgaac cagtaattca ccagacaatg gcgcaccact
     2281 taaataaact tgcccgtcat cacccaccat actggttatt ccggtactgt tttcccgtac
     2341 cagtgatcca aacggcaccg gttttccgcc ctgggtaacg gtaatgagcg cccgcacgcc
     2401 tatgcgggta tcaaaattgg cacgaaccaa cgcgccttgc gtcggcacaa cgctgctaat
     2461 atttttttca acatcgatgg aattccccat cgtattggta tcaagcgcga tacggttata
     2521 ccgataaacc gtggcatacg gcatcaccgc atagccgcgc cagtcggtta aaatgccagt
     2581 ttgattttca atgcgtacac cgcctgcgcc aggcgcttta atcaaaacat tggtatcccc
     2641 taaaggctgg ctcagtgtta tgccattttc atgcccgacc acaccgccag acagctgcca
     2701 gttaacgtca tgttgatcgc ggtcgtagtt atacccgacc cccagcgtac cgtaagtggc
     2761 ctgccagttt gccgtggcgc tgcccgtgta cccattattc gaggtatcgc cctggcttac
     2821 gtgataactc aggttgtggc cttccagtaa ggtcccacca acgcctgcca gccagctatt
     2881 ttgcccgttg ctgttacggt tagcgttcaa ggaggcataa gcgcggtcga gcgcattttc
     2941 ccgggtgtag cgacgtttgg tcaaaacatt gaaaggcact gaaacattaa gtccgacaat
     3001 atgttcgtta tcggggatcc ctacagattc attccacgaa aatgagagtg aataactgat
     3061 gccaacccag ctgctggtat accccacctg ataccaggta tctgaatccg aagtattcca
     3121 gtatttttga tgggtaccag agatataaag cgagccaaag tcattaagtg attgtgaaat
     3181 atttaactgc aaacggtctt tgcggctaaa gcgtaaattg tggtaattca cgattatcgg
     3241 ttcatcgcga tgctcaccgt cgtaatcgta atcatattcg tacccctcca ttcgacgata
     3301 cgcaacatca tcaagggtat aaaaaccttg tgtcgaatag cggtaaccca ttaactgaaa
     3361 attggtgccg aaggtgtaca tcgatttcgc atagaggaag cgaatggaat ccccctcatg
     3421 acgactgtcg tcggctaact ggctgcgcgc atgggtcaca tccagcgaca ctgcgcccca
     3481 gttcccgagg ttgcgcccca gccctaataa aaaggcggtg taattggcag ataattgcgt
     3541 cccgccgtag gcagtaaatt cctgtggtaa accaccgagt gccgtgccct gaaaaaagaa
     3601 aggtgatgat tgctgactat taccgctacg aaaatcgccc gccgtcaggt caaatttgaa
     3661 acgcccttcg cgttgtaaaa tcggcactgt tgaatacgga attgtgtaat tctgccatcg
     3721 cgctcgtcga tggtgacatc cagatcgcca tttgaagatg tcgggtgcaa atcagtaatt
     3781 tcaaaagcac cgggggaaac atagctttga tagataataa aaccattttg ccgaatcgtt
     3841 agctgggccg ccgtacgggc aatcccacgt accgttgggg caaacccttg ctggctatct
     3901 ggatacatat tatcggaaga ataaagccgt acacca
//
LOCUS       DAQSWQ010000210         3924 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.212, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000210 DAQSWQ010000000
VERSION     DAQSWQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.212"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..303
                     /locus_tag="R8G18_004223"
     CDS             <1..303
                     /locus_tag="R8G18_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797764.1"
                     /translation="PRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYS
                     NNRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            392..2260
                     /locus_tag="R8G18_004224"
     CDS             392..2260
                     /locus_tag="R8G18_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HEE9797765.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2297..3924
                     /locus_tag="R8G18_004225"
                     /pseudo
     CDS             2297..3924
                     /locus_tag="R8G18_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT         1231 a          723 c          769 g         1201 t
ORIGIN      
        1 ccacgtgtag aactgtatta ccgctttaac catgaagaag aatattaccc ttctcaccgt
       61 tttatgcttg ttgttgatat gaagacagta atggtttttt cacaacgagc tgaatcactg
      121 gggttatact cgaataatcg cttgcttatc aatataattg ccgaacatat ccatagtgat
      181 atatatctaa ccaagtatca gcaacaatat ccagatgcca atgcattaat aaatactttg
      241 catgaatctg gcaatgatat ggtgcaggat gaaaaacatt tgagtgatag caaaaaccgt
      301 taatattttc ctttcattgt gatcgtctgt aggcgatcac aatctatttg tcttttataa
      361 cgtctaaact tttttatgca aaggtgtatt tatgattttt tttaacgata taaagcccac
      421 tggttggtta aagaataaga tggaatggta tatgaacggt catataggag aactggataa
      481 attagttccc catcttatta cggagcataa aatatatgga agagatcgta taactccacg
      541 cactgtatct gctgaagatg gcgttattaa aaaacagaat aatcccgatg ataacatgat
      601 gcaatattat tggtgggata gtgaaactca atcgaattgg aaggatggat attgccgcag
      661 tgcctacttg ttagataata aagaatggca ggaaaaagcg agcgatttat gtttggaact
      721 tatcgatacg caagatgatg atggctacat tggaatttat gattcatcat taaaatttaa
      781 ctgtaaagca gaaaacggag agttctgggc acaagctact gccttgcgtt ttgtgcttgg
      841 atgttatgag tccacccgta atgagagttt attaacttct cttacccaag ccgcacaatg
      901 cattatggct ggctacccta aagaaacgtc acatcctttc gttgtggaac agggttttgc
      961 tggacatagc cacggattaa caataacaga tgttttctac cgcctttatg aaataaccaa
     1021 taacattgaa tatctctcat attgcttgtg gctgtatgaa gattattccg caggatgcgt
     1081 gtcggagcaa gatctgcaat ataaaaatgt catagaccct ggttaccttc ttaaaggtca
     1141 tggcgtacat acgtatgagc atattagggc attagccatc gcagcgagcc aacggccgca
     1201 gtatcaaact gcattagata tttttttatc ccagttgaaa tattatctta caccatctgg
     1261 cgctcccatt ggtgatgagt ggattacagg acgcacagct aatgccactt atacaggcta
     1321 tgaatattgt tctgttcatg aactatttag cacttatgtt atgttaataa aactgaccac
     1381 taatattggt ctggccgatg atgctgagtg gctattctat aatgctagtt ttggcatgca
     1441 gcatcccata cttcacgcga taatgtactg taagactgat aattgttatg aagcaaatga
     1501 aaggaaacat cctgacagta atcagtttaa taccagatat aaatactccc ctactcatga
     1561 ggaggctgct gtctgctgcg taccgaatac agcaagaacc ataccggctt ttgtcgaaaa
     1621 tatgtttcaa gaaaataaca atggttttac tgcattattt tatggcccgt gtgagtttta
     1681 tggcacattt aataatgttg caattaaaat tacacaatta actgaatacc ctcatgattt
     1741 aagcgtaaaa tgtctcattg agcctgagtc atctgttcgt tttgctctca gtttccgtta
     1801 ccccggctgg gcaaaaatga tgattattaa tggtgttact tttactaaaa atgatactca
     1861 aaactcactt attattttag atagaacatg gcaacatcat gatgtgattg acattaaatt
     1921 tatagctgat atacagttta atactgactt atgtggtgac acttatattt ccagaggtcc
     1981 cgttttatat gcaattgagc ttgagagcga tattttaatc aaaaaaaacc tgctgaacgg
     2041 gaaatatttt gactcaggat acataccctg tagtagagct gatgaatcta tccagttctc
     2101 ttatgctgat atggaatctt tttcatattc aacctcacca gagggcaaac aatacattga
     2161 aggctgtttt tatttaaaca agactaaaat agtcagaaaa cttataccat ttggtcaaac
     2221 cattcttcgc aaagtaactt tctccaatat agagagttaa tattttcgag tgttataatt
     2281 aattaaaggg atttctatgt accttactga taatattatc aaactgtcga ataaaatagg
     2341 tcagcgtgtt tatactgttg tctctgaaat gtcgattgaa gcatggataa caaaagaacc
     2401 gcagcctttt gtttatcgcc gtagcggcgc ttaccagaaa ctcaccattg gctcagactg
     2461 gggtcaactg tttgactgtg cctggatgcg agtttatggt aaacgtccag cagataaatt
     2521 tagacacaaa ctcgtcgctc tcgttgatat cggtggcgaa ggtcttattg tggataagaa
     2581 tggttcaccc atctgcggca tcacgaataa agcatcatct tatggcgtac cgccagataa
     2641 accaggtaaa tgggtggttg acctttctct cgttagtgaa aatgacgagg ttgagttttg
     2701 gattgatgct gcctgcaacg atttatttgg ctatgttact aatggaggga ttattactga
     2761 tgttcatatc gcgacacgta atcaactact gaaatccctt tattatgata ttgaggtgct
     2821 tttcgactgg attaatgatg gtcagaagtt tgaatccata catcctaaag gtatcacccc
     2881 agaacaattt attgccaaaa gaagtgagcg tactgacgaa atcataagta ctctcgaata
     2941 tattgataat acgttaatta cctttagcaa tgaagaaata attaaatgtc aaattgcaat
     3001 ccaatccata atcaataaaa acaataatcc ttcagagttc aggattatgg ctactggtca
     3061 tgcccattta gatatagcgt ggatgtggcc tttaagagaa ggacgcagaa aagcaataag
     3121 aacatttgcc accgcccttg caaacattga aaaatatcct gattacattt ttggcgctag
     3181 tcagtaccaa ctttttcact ggataaaaaa agattatccc tatttcttcg aaaagttaaa
     3241 aaaacaaatc gccgcaggcc gctttgagtt acaaggttgc ttctgggtcg aatgcgattt
     3301 aaatctggta agtggtgaat cgctgataag gcaaatcatg catgggacac gattcacact
     3361 gcagaatttt tcaaaaaaga taaattatgt ctggcaaccg gatgtctttg gattccccgc
     3421 aacgcttcca caaattctca aaaaatcagg cattaactat attgcatcgc aaaagctctc
     3481 acaaaacaag attaataaat ttaataatta ccttttccgc tggcagggcc tggatggctc
     3541 agaaattctg atgcataact ttcctgaaga cacttatgat agccgagcac gtgctcgctc
     3601 tttagaatat attgaacaaa actataatga aaaagagatc tgtccctatg cgcttatggt
     3661 gtatggtgta tggtgtcggt gatggcggcg ctggccccgg tgaggaacat atagaacgat
     3721 taacacgtat acgcaatatt gatggcttac ctcatgttga tttcagccgt gttgataagt
     3781 tcttcactta cgccgacgcc tttcgcgaaa gcttgcccat tatttcaggt gaactttatt
     3841 tcgaggctca tcaagggtgc tttacatcag agtcagcaac taaagctcat aatcgaaata
     3901 tggaaaataa attacatgat gctg
//
LOCUS       DAQSWQ010000211         3908 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.213, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000211 DAQSWQ010000000
VERSION     DAQSWQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.213"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            24..590
                     /locus_tag="R8G18_004226"
     CDS             24..590
                     /locus_tag="R8G18_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEE9797766.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="R8G18_004227"
     CDS             594..1169
                     /locus_tag="R8G18_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEE9797767.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="R8G18_004228"
     CDS             complement(1211..2263)
                     /locus_tag="R8G18_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEE9797768.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="R8G18_004229"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="R8G18_004229"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HEE9797769.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3777
                     /locus_tag="R8G18_004230"
     CDS             3580..3777
                     /locus_tag="R8G18_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEE9797770.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          987 c          952 g          998 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta tgcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAQSWQ010000212         3835 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.214, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000212 DAQSWQ010000000
VERSION     DAQSWQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.214"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..268)
                     /locus_tag="R8G18_004231"
     CDS             complement(<1..268)
                     /locus_tag="R8G18_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEE9797771.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLD"
     gene            355..762
                     /gene="nudG"
                     /locus_tag="R8G18_004232"
     CDS             355..762
                     /gene="nudG"
                     /locus_tag="R8G18_004232"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HEE9797772.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(728..1000)
                     /locus_tag="R8G18_004233"
                     /pseudo
     CDS             complement(728..1000)
                     /locus_tag="R8G18_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            1236..2579
                     /gene="gdhA"
                     /locus_tag="R8G18_004234"
     CDS             1236..2579
                     /gene="gdhA"
                     /locus_tag="R8G18_004234"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEE9797773.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2696..3736)
                     /locus_tag="R8G18_004235"
     CDS             complement(2696..3736)
                     /locus_tag="R8G18_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HEE9797774.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT         1001 a          932 c          916 g          986 t
ORIGIN      
        1 aatcaagaga aatatcaaga aagccgcccg cgtcggtaag ctctctgcgc cgcgccagcg
       61 caccgtccag accatcaagc agcctgttca acaaaatgac gactagcgcc gccagatacc
      121 agcccagcgc cagaaacggc agagccagca cgccgatggc aaatcccact aatgttaaac
      181 catctggcgt aatgcccggt ttatcaagaa cccgcacgca ctgatgcaac aacggtttaa
      241 tccggggatg aagatggcgg tctagcacag gcactcctta aatataaagc ctttctgatt
      301 gagcaacagt gcggatatta tggcattttt cgcttatctg cccgtgtgta atttatgaaa
      361 atgattgaag ttgttgccgc catcattgaa cgtgatggca aaattttact cgcgcaacgc
      421 cccgcccaga gcgatcaggc gggattatgg gagtttgccg atggtaaagt cgagctggat
      481 gaaagccagc agcaggcact ggtgcgtgag ttaaacgaag aattgggcat cgaagcaact
      541 gtgggtgagt atgttgccag ccatcagcga gaagtttcgg gccggattat ccatcttcat
      601 gcctggcacg tacccgactt ccacgggacg ttacaggcac atgaacatca ggcgctggtc
      661 tggtgctcac ctgaagaggc gctgcaatat ccgctggccc ctgctgacat tccactatta
      721 gaggcgttta tggctttacg cgccgccaga gcagcggatt agtgctaagg gttttgtcat
      781 cccgctggca ttacagcagt attccttcgg ctttaattac cgccccttca gaataatttt
      841 gatcctgata aacgcagcac tgagtacagg gctgcgctga ctgcccgcct gaactgaaca
      901 cttctggcgg tacgtttacc tccacgtccg gacgataatg cgggttagcc agtgcaatta
      961 atggaaatgc taatactacg gcgaacaatg ctcgactcac agggaactcc ttaacgttat
     1021 tgtctctgca actgataacg gtagccgggt ggcaaaactt tagcgtctga gttatcgcat
     1081 ttggttatga gattactctc gttattaatt tgctttcccg ggtcattttt ttcttgctta
     1141 ccgtcacatt cttgatggta tagtcgaaaa ctgcaaaagc acatgacata aacaacataa
     1201 gcacaatcgt attaatatat aagggtttta tatctatgga tcagacatat tctctggagt
     1261 cattcctcaa ccatgtccaa aagcgcgacc cgaatcaaac cgagttcgcg caagccgttc
     1321 gtgaagtaat gaccacactc tggccttttc ttgaacaaaa tccaaaatat cgccagatgt
     1381 cattactgga gcgtctggtt gaaccggagc gcgtgatcca gtttcgcgtg gtatgggttg
     1441 atgatcgcaa ccagatacag gtcaaccgtg catggcgtgt gcagttcagc tctgccatcg
     1501 gcccgtacaa aggcggtatg cgcttccatc cgtcagttaa cctttccatt ctcaaattcc
     1561 tcggctttga acaaaccttc aaaaatgccc tgactaccct gccgatgggc ggtggtaaag
     1621 gcggcagcga tttcgatccg aaaggaaaaa gcgaaggtga agtgatgcgt ttttgccagg
     1681 cgctgatgac cgagctttat cgtcatctgg gcgcggatac cgacgttccg gcaggtgata
     1741 tcggcgttgg tggtcgtgaa gtcggcttta tggcggggat gatgaaaaag ctctccaaca
     1801 ataccgcctg cgtcttcacc ggtaagggcc tttcatttgg cggtagtctt attcgcccgg
     1861 aagctaccgg ctacggtctg gtttatttca cagaagcaat gttaaaacgc cacggtatgg
     1921 gttttgaagg gatgcgcgtt tccgtttctg gctccggcaa cgtcgcccag tacgctatcg
     1981 aaaaagcgat ggaatttggt gctcgtgtga tcactgcgtc agactccagc ggcactgtag
     2041 ttgatgaaag cggattcacg aaagagaaac tggcacgtct tatcgaaatc aaagccagcc
     2101 gcgatggtcg agtggcagat tacgccaaag aatttggtct ggtctatctc gaaggccaac
     2161 agccgtggtc tgtaccggtt gatatcgccc tgccttgcgc cacccagaac gaactggatg
     2221 ttgacgccgc gcatcagctt atcgccaatg gcgttaaagc cgtcgctgaa ggggcaaata
     2281 tgccgaccac catcgaagcg actgaactgt tccagcaggc aggcgtactg tttgcaccgg
     2341 gtaaagcggc taatgctggc ggtgtagcaa cgtcgggcct ggaaatggca caaaacgctg
     2401 cgcgcctggg ctggaaagcc gagaaagttg acgcacgttt gcatcacatc atgctggata
     2461 tccaccatgc ctgtgttgag catggtggtg aaggtgagca aaccaactac gtgcagggcg
     2521 cgaacattgc cggttttgtg aaggttgccg atgcgatgct ggcgcagggt gtgatttaag
     2581 ttgtaaatgc ctgatggcgc tacgcttatc aggcctacaa atgggcacaa ttcattgcag
     2641 ttacgctcta atgtaggccg ggcaagcgca gcgcccccgg caaaatttca ggcgtttatg
     2701 agtatttaac ggatgatgct ccccagggaa catttcttat gggccaacgg catttcttac
     2761 tgtagtgctc ccaaaattgc ttgtcgtaac gataacacgc ttcaagttca gcatccgtta
     2821 actttctgcg atagcagcag atatgccagt aaagaaaccc cattagacta tttttttgat
     2881 aatcttcttc gctttcgaac aactcgtgcg cctttcgaga agcaagcatt atataatgcc
     2941 aggccagttc ttcttcaatt gtcccgtttt gaaaagctgt gcttgatatc gaggtcatcc
     3001 atgataattc cgccgcccat attagcttcg ccgaggattt accggagcga ttattagcgc
     3061 aatcagagat atagtctgag ggaaaaacag caaatttatt caacaaggcg ataacctgct
     3121 ctggggcttc ctccatgttt gctttaaagg tattggctcc atggtcgcca gaaagaaaat
     3181 gctccattaa ggcacaataa ctttcgctat cttcgatacc ccattgattc tctaaagact
     3241 cgcgtctttt actgatgata tcgatcgagt caaaaggaag cacatgatat tggaaggtat
     3301 ctttaccagg ttcaggcttt cgcggccaga actctagcgt ttcagaccat tgcttatgat
     3361 agaatcgata aggtgcgatc aattgtagcg cctgtaactt ctcgatactg agcggctcaa
     3421 tacctttagc ctgataataa tgcagttgtt ctttttttgc tttaaaaccg gcccgaacaa
     3481 taagccccat cataattaat agataaagaa aagagcatcc cgcggtaatc aggcctcttt
     3541 cattcaaacc gttggatgtt atcgctgcga acacaaacat tacagcgaca acacatgtta
     3601 aataaaaccc ccacttacaa agcagcatgg ccttattttc tttaatcatc cgttcaaaat
     3661 tactattaaa tatttcccag ccattaaaag aatacttctc gctcccagga tggttttgta
     3721 ataaaacttt tttcatcaca ttccctgtta ttacatactt tagaggcgat aattttatcc
     3781 atgcaaaaaa aatatccaac gaattttctt gatccgctgg atatttcaga atatg
//
LOCUS       DAQSWQ010000213         3797 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.215, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000213 DAQSWQ010000000
VERSION     DAQSWQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.215"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="R8G18_004236"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="R8G18_004236"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEE9797775.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="R8G18_004237"
     CDS             1027..1485
                     /locus_tag="R8G18_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEE9797776.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="R8G18_004238"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="R8G18_004238"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HEE9797777.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="R8G18_004239"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="R8G18_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HEE9797778.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="R8G18_004240"
     CDS             2691..3368
                     /locus_tag="R8G18_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HEE9797779.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="R8G18_004241"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="R8G18_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEE9797780.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          903 c          990 g          953 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 tcacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAQSWQ010000214         3734 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.216, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000214 DAQSWQ010000000
VERSION     DAQSWQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.216"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..182)
                     /locus_tag="R8G18_004242"
     CDS             complement(<1..182)
                     /locus_tag="R8G18_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HEE9797781.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="R8G18_004243"
     CDS             426..683
                     /locus_tag="R8G18_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HEE9797782.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="R8G18_004244"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="R8G18_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HEE9797783.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="R8G18_004245"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="R8G18_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEE9797784.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="R8G18_004246"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="R8G18_004246"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEE9797785.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3734)
                     /locus_tag="R8G18_004247"
     CDS             complement(3308..>3734)
                     /locus_tag="R8G18_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEE9797786.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          952 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaat
//
LOCUS       DAQSWQ010000215         3674 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.217, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000215 DAQSWQ010000000
VERSION     DAQSWQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.217"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..95
                     /locus_tag="R8G18_004248"
     CDS             <1..95
                     /locus_tag="R8G18_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HEE9797787.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="R8G18_004249"
     CDS             244..1491
                     /locus_tag="R8G18_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HEE9797788.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="R8G18_004250"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="R8G18_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HEE9797789.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="R8G18_004251"
     CDS             complement(2343..2687)
                     /locus_tag="R8G18_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797790.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..3531
                     /locus_tag="R8G18_004252"
     CDS             2929..3531
                     /locus_tag="R8G18_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797791.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          919 a          740 c          845 g         1170 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctaggat tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatggg gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacagacc gcgcgtgatg atactgaatg agatagtata
     3541 tagcgggcaa gttttagtta atttatcgag gtaatataat ttacctcgac tcgtttattc
     3601 tggtatgaat atttcgcttt acgatcgatt tttatctgat gatatcatgc ggttttcata
     3661 tactgactta ctgt
//
LOCUS       DAQSWQ010000216         3627 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.218, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000216 DAQSWQ010000000
VERSION     DAQSWQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.218"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(89..652)
                     /locus_tag="R8G18_004253"
     CDS             complement(89..652)
                     /locus_tag="R8G18_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEE9797792.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="R8G18_004254"
     CDS             complement(756..974)
                     /locus_tag="R8G18_004254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797793.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="R8G18_004255"
     CDS             complement(1133..1519)
                     /locus_tag="R8G18_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HEE9797794.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="R8G18_004256"
     CDS             complement(1509..1880)
                     /locus_tag="R8G18_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEE9797795.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="R8G18_004257"
     CDS             complement(1880..2137)
                     /locus_tag="R8G18_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797796.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="R8G18_004258"
     CDS             complement(2134..3525)
                     /locus_tag="R8G18_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEE9797797.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="R8G18_004259"
     CDS             complement(3522..>3627)
                     /locus_tag="R8G18_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEE9797798.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAQSWQ010000217         3626 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.219, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000217 DAQSWQ010000000
VERSION     DAQSWQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.219"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..401)
                     /locus_tag="R8G18_004260"
     CDS             complement(<1..401)
                     /locus_tag="R8G18_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEE9797799.1"
                     /translation="METNKKKLTRILKKVLPTIQPPKIQKTSEWISNGVVKFVDGPNM
                     GLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIAFNLMANDPCNFAFGS
                     SSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
     gene            complement(316..723)
                     /locus_tag="R8G18_004261"
     CDS             complement(316..723)
                     /locus_tag="R8G18_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEE9797800.1"
                     /translation="MRDTNTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQ
                     VLTEYLFQVKTAVRAIPSKTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDD
                     MEVIEDGNKQEEINEDTEESTTDDTTAEDTENE"
     gene            897..1460
                     /locus_tag="R8G18_004262"
     CDS             897..1460
                     /locus_tag="R8G18_004262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797801.1"
                     /translation="MSDRNNDFSLLVKLLNGFLKKDEIKKRLYSFVKKETNDWEKWLQ
                     IEFAHYLTSKRKYELHREVTIYLDPIDYPDSKLAKVDLILREKSTPSDDYIFIEFKCT
                     KGVVPLVRGLNADKNKISAIRQCNYKMRNYLGVGFHLFCEPDDIEFMEKYVTEELKGS
                     YAVFKLCECPVRYNCQCEFNEIGVVLF"
     gene            complement(1606..1908)
                     /locus_tag="R8G18_004263"
     CDS             complement(1606..1908)
                     /locus_tag="R8G18_004263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797802.1"
                     /translation="MEKLILLLLLPLTTSAAIPDFKCWDIHNDSFSTDMSFYVTRYLG
                     KMPPPYGAFHYETAPDEKFYRELVITNEAFDDGYNYGSRYLNKEGTLYDDIKCKRL"
     gene            complement(2128..2568)
                     /locus_tag="R8G18_004264"
     CDS             complement(2128..2568)
                     /locus_tag="R8G18_004264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797803.1"
                     /translation="MKITVDNISEMFAGTKELLIGGLITGIVGVLILTRVSGYEYVKV
                     PLMLVMIFLLTVVPGFIAMLFSPLTKVFAKIFDIKSSNILCRIFSFLYFILYMAITFL
                     VTNFIFTTAMSLHGGDGGLRACFAIGCFVVAVEQTRKFYSLLIK"
     gene            complement(3319..3615)
                     /locus_tag="R8G18_004265"
     CDS             complement(3319..3615)
                     /locus_tag="R8G18_004265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797804.1"
                     /translation="MKVHNQQWLDDLGVHGGSAIPLVTGDFEVARLNRRYTEENIDRL
                     IFPVLIKSIRQYCEKVIVPVRDKTYYPILREAGVWAVQGEFRPMRFEQCEKLIG"
BASE COUNT         1091 a          744 c          611 g         1180 t
ORIGIN      
        1 ttcttatcgg ttactaacgg ctttagtact gaactggttt caatagctgg aagaaattta
       61 ccatccttga atttcttcac ttctgattca gaactactac caaaagcaaa gttacaggga
      121 tcattagcca ttaggttaaa tgcaattgat tgtagtactg tagttttcaa aagctgggaa
      181 caagattgaa gtacaatctt tttagtactt cgctcttgag ctatatccat tggttctttt
      241 tggaaactaa acggaaccca atcaaggccc atattcggcc cgtccacaaa tttaactaca
      301 ccattactaa tccactcact cgttttctgt atcttcggcg gttgtatcgt cggtagtact
      361 ttcttcagta tcctcgttaa tttcttcttg tttgtttcca tcctctatta cttccatgtc
      421 atcgggtaac tcaaattcca ttgagcctaa ttggtataat gttttatcaa tatgcgttct
      481 taatacatcg cgtaaatcct ttgcatctga ttgtgcaaat aattcaagat aagtttttga
      541 tggtattgca cgcactgctg ttttgacctg gaatagatat tccgttagta cctgttctat
      601 atattcagta ctgaccacca gcccattctt ttctgctaat tctaattctg ctaactgccg
      661 ttctgctgaa agcttcttca aacgctcttg ttcaatttgt tcttttgtat tagtatcacg
      721 caacggcttt agtacattat ctacaatcca acttcttgcg tttgaatcag tgtctgtcgg
      781 cattcccttt tctacccaag ttcgaactgt agattcatcg tatccgtact gtctcgctaa
      841 tgaccttaat gagatatcca tatttgttcc tttttatttt atttataggg tttacaatgt
      901 cagaccgtaa caatgatttt tctctgttag taaaattgct gaacggattt ttgaagaaag
      961 atgagataaa aaaaagacta tattctttcg tgaaaaaaga aactaatgat tgggaaaagt
     1021 ggctgcaaat agaatttgct cattatctaa cttcaaaaag gaagtacgag ctacatcgtg
     1081 aagttacgat atacctcgat ccaatagatt atcctgatag taaacttgct aaagtagatt
     1141 tgatactgcg tgaaaaatcc acaccctctg atgattatat ttttattgag tttaaatgta
     1201 ctaaaggtgt agtaccatta gttaggggct taaatgctga caaaaataaa attagtgcta
     1261 taagacagtg taactacaaa atgcgtaatt atcttggtgt cggatttcat cttttttgtg
     1321 aaccagatga tatcgagttt atggaaaagt acgtaactga agaacttaaa ggctcttacg
     1381 ccgtgtttaa attatgcgag tgtccagtaa gatataattg ccaatgtgaa tttaatgaaa
     1441 ttggtgtggt acttttttaa atgcggtgcg ggtatgatca ttttgttcac acacaattga
     1501 ttaaacccgc tgccgaaaac cctcgatagt accgccagta tggagggacc cattgaatgc
     1561 aatggttaat atactttgta agctatatac aatgaatcat gagtatcaaa gccgcttaca
     1621 tttaatatca tcgtacaaag ttccttcttt gtttagatat ctcgaaccgt aattatatcc
     1681 atcatcaaat gcttcattgg ttattaccag ttccctgtaa aatttctcat caggtgctgt
     1741 ttcataatga aatgcaccat aaggcggcgg cattttaccg agatatcttg taacgtagaa
     1801 actcatgtca gtactaaatg aatcgttatg aatatcccag catttaaagt ctggaatagc
     1861 tgcacttgtt gttaaaggta ataataatag caatatcaat ttttccataa gagataccaa
     1921 tgcaaatgat ttaattgcat gataacacct acaaagtctc aataccaatt aacaagcgat
     1981 atggctgatt ttgtttcaat tacgtaaaat aatttaaaat atcgttaatt aaaacaattg
     2041 tgatgaaatg ctcaccttgt aatttgttgc gtatttgatg aaagtactta cccttcatca
     2101 aatacacatt tctttagtac ttgcccttta cttgataagc aaactataga attttcttgt
     2161 ctgctcaact gcaacgacaa aacagccaat tgcaaaacat gctctcaacc caccatcccc
     2221 gccgtgtaat gacattgcgg tagtgaatat aaaattagtc accaagaaag taatagccat
     2281 atacaagatg aaatatagaa atgagaaaat gcggcaaagt atattactgc ttttaatatc
     2341 aaatatcttt gcaaatactt tcgttagtgg tgaaaaaagc atagcaataa aaccaggaac
     2401 tacagttagt aagaatatca ttactaacat taaaggtact ttaacgtatt cataaccact
     2461 aacacgagtt aaaataagta ctccgacgat acctgttatt aagccaccga ttagtagttc
     2521 tttagttcct gcaaacattt cagagatatt atcgactgta attttcattc tactaactat
     2581 tccttcataa aaagttcact ccgatcttat caccgcataa agaaaaagcc ctgtagattc
     2641 tcaccataca gcctaagcac taaacttttc ggattacttc gatactacct gccattgtcc
     2701 atcatgccgg atgatctcat actggtactt accatcacac cgtacatcaa tacgtcctgt
     2761 accattctca gtactatcca tagtcaccat atcgcatggg tacttaccct tgattagtga
     2821 ctgtgcgaaa tccatagcgt tctggttctc attgtccgtg tactgtggct ggggtgcatc
     2881 ctcacctgca atgttgaaga gtaccccagc cagtactaac aaaatcacca ttacctttcc
     2941 catacgtacc ctatagattg tcgaggatat gggatggtga attgacccct tctaaccaat
     3001 cgtgaacact atcggtctga tgtggtacta ccttacttac atgctctcta tagggatgta
     3061 gttcagcaat gcgggaatct cgtatgaggc atccagtact tgtattgtca tcatgataga
     3121 tcttgttata gatgcgaccg caatcactct ggaactgagg acggtagtac ttcaccccat
     3181 tacgtgttcc tgcatcaaac agtactacgg ccttgtcatc tacatagcca acgtaagctt
     3241 tatccatgaa gctgtgcgta gtagtacatc cagtactaag ggcaatcagt aacccaatta
     3301 gtaatttttt cattattgtt atcctattaa tttttcacat tgctcaaaac gcattggcct
     3361 gaactctccc tgtaccgccc atacaccggc ctctctgaga attgggtaat acgttttatc
     3421 tctgacgggt acgatcacct tctcgcagta ctggcgaatg ctttttatga gtactggaaa
     3481 gatcagacgg tcaatgttct cttctgtgta ccttctattt aatcgggcaa cctcgaaatc
     3541 acctgtaacc agaggaatag cagaaccacc atgtacaccg agatcatcaa gccactgctg
     3601 gttatgtact ttcatatccc ttgtca
//
LOCUS       DAQSWQ010000218         3564 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.220, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000218 DAQSWQ010000000
VERSION     DAQSWQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.220"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            168..503
                     /locus_tag="R8G18_004266"
     CDS             168..503
                     /locus_tag="R8G18_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEE9797805.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            496..978
                     /locus_tag="R8G18_004267"
     CDS             496..978
                     /locus_tag="R8G18_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797806.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="R8G18_004268"
     CDS             complement(1012..1092)
                     /locus_tag="R8G18_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797807.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /locus_tag="R8G18_004269"
                     /pseudo
     CDS             complement(1128..1552)
                     /locus_tag="R8G18_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1982..2472
                     /locus_tag="R8G18_004270"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="R8G18_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2697..3188
                     /locus_tag="R8G18_004271"
     CDS             2697..3188
                     /locus_tag="R8G18_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEE9797808.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT         1347 a          569 c          567 g         1081 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatgtt attagtttgt agtgaacagt acttttacca ataatgaaaa
     2581 atataccata agcaatgcaa gcttaaaact aataatttaa aaaagataaa tgtaaaaata
     2641 ttaatgagtc aatcaataat aatttagcat cacgagacac atcacagagg aatattatga
     2701 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
     2761 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
     2821 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
     2881 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
     2941 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
     3001 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
     3061 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
     3121 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
     3181 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
     3241 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
     3301 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
     3361 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
     3421 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
     3481 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
     3541 ttctcatatc attttcttga ggga
//
LOCUS       DAQSWQ010000219         3543 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.221, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000219 DAQSWQ010000000
VERSION     DAQSWQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.221"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..140)
                     /locus_tag="R8G18_004272"
     CDS             complement(<1..140)
                     /locus_tag="R8G18_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797809.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /locus_tag="R8G18_004273"
     CDS             complement(186..527)
                     /locus_tag="R8G18_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797810.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2150)
                     /gene="xylB"
                     /locus_tag="R8G18_004274"
     CDS             complement(696..2150)
                     /gene="xylB"
                     /locus_tag="R8G18_004274"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEE9797811.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="R8G18_004275"
     CDS             complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="R8G18_004275"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEE9797812.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          925 a          982 c          783 g          853 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccccgta
      901 cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc
      961 gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc
     1021 atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg
     1081 ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta
     1141 cgctcgccgg aaagataagg cagaaaccaa actggctcga cactttcatc agcctgttga
     1201 gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc ccaatccaga
     1261 cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag cgcatggcaa
     1321 aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc
     1381 cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac accaactgca
     1441 ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc ccacgctttc
     1501 gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg
     1561 tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc
     1621 agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga
     1681 cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc cggctcatgc
     1741 cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc
     1801 actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg
     1861 ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt tgctccatgc
     1921 atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagagct
     1981 ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga
     2041 tgcgggcgcg aaacggtcag cttttccgtt tgcgaatcaa ccacctcacc ctgctcgttg
     2101 agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt
     2161 ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc
     2221 gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct
     2281 gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt
     2341 ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc tgcgcgatgc
     2401 gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc agtgccatcg
     2461 tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta
     2521 ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca
     2581 tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg
     2641 catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt
     2701 catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct
     2761 ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg taatcatatt
     2821 gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctgg aagccgattt
     2881 tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca
     2941 agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac
     3001 cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga
     3061 cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag
     3121 cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg acatcaacca
     3181 tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat
     3241 cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt
     3301 tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca
     3361 caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca
     3421 agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga cggaatgcta
     3481 acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca aaataggctt
     3541 gca
//
LOCUS       DAQSWQ010000220         3369 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.222, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000220 DAQSWQ010000000
VERSION     DAQSWQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.222"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="R8G18_004276"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="R8G18_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HEE9797813.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="R8G18_004277"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="R8G18_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="R8G18_004278"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="R8G18_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            2608..3145
                     /locus_tag="R8G18_004279"
                     /pseudo
     CDS             2608..3145
                     /locus_tag="R8G18_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            3187..>3369
                     /locus_tag="R8G18_004280"
     CDS             3187..>3369
                     /locus_tag="R8G18_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797814.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
BASE COUNT          948 a          731 c          804 g          886 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 ctatcaataa acaacagtag ttaaacttac tgttatttaa aatatttact ttgtagcaag
     2461 gttttcctat taaaagagaa ccaacattta ttacaaacgc attaagtctg gaaacccggc
     2521 gactcgccaa tgtattagca acattaatcg attgtctgat gtaacggttt tcccgcttat
     2581 cctcttaaca catggagaat gcatcagatg caaactacgc gtacccccta ctctatttcc
     2641 tttatggcga cagtgttact tttattgctc tttgcttgcc atagcaccgt tgccaacgca
     2701 gctgtcgcac tggaatacgc tgcggataat caacgctact aatcatcagt taccaggcga
     2761 ccgtgaaagc ctgttctggg tgaatgttaa agctattcct gctatggaaa aagaccagaa
     2821 gaatgagaat acattacagc tggcaattat tagccgcata aaaatgtttt atcgccccac
     2881 gaacctggca atggcacctg aagaagcgcc tgcaatgctt cggtttcgtc gtagcggtag
     2941 caaactcact cttattaatc caacgcccta tttcattacc gtaacaaata tgaaagcagg
     3001 aaatagcaat ctcccaaaca ccatggtgcc gccaaaaggt gaagtatcgg tggatatccc
     3061 gcatgctgca accggggata tcagttttca gacgatcaat gattacggtg cattaactcc
     3121 gcgtattaag gcaaccatgc agtaagccag tgacctaatt aataataaac tcaatcggaa
     3181 ttaataatga cagcctttcg tgccgcattt aaggcatatc gtatgcatca ggtgttaata
     3241 ctgcctcgct ttgcacggct taccattgcc cttggcttag caactgcggt atttcccgtt
     3301 gacgcagagt attattttaa tccacggttt ttaagcaatg atcttgctga atctgtcgat
     3361 ttatccgcc
//
LOCUS       DAQSWQ010000221         3322 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.223, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000221 DAQSWQ010000000
VERSION     DAQSWQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.223"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="R8G18_004281"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="R8G18_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HEE9797815.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="R8G18_004282"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="R8G18_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEE9797816.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="R8G18_004283"
     CDS             complement(1250..2824)
                     /locus_tag="R8G18_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HEE9797817.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="R8G18_004284"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="R8G18_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          852 a          789 c          713 g          968 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagcg ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 taccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAQSWQ010000222         3237 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.224, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000222 DAQSWQ010000000
VERSION     DAQSWQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.224"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(91..618)
                     /gene="mobB"
                     /locus_tag="R8G18_004285"
     CDS             complement(91..618)
                     /gene="mobB"
                     /locus_tag="R8G18_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEE9797818.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(600..1184)
                     /gene="mobA"
                     /locus_tag="R8G18_004286"
     CDS             complement(600..1184)
                     /gene="mobA"
                     /locus_tag="R8G18_004286"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEE9797819.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1254..1523
                     /locus_tag="R8G18_004287"
     CDS             1254..1523
                     /locus_tag="R8G18_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HEE9797820.1"
                     /translation="MKCKRLNEVIELVQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1600..2586
                     /gene="srkA"
                     /locus_tag="R8G18_004288"
     CDS             1600..2586
                     /gene="srkA"
                     /locus_tag="R8G18_004288"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HEE9797821.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2603..3229
                     /gene="dsbA"
                     /locus_tag="R8G18_004289"
     CDS             2603..3229
                     /gene="dsbA"
                     /locus_tag="R8G18_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HEE9797822.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          827 a          780 c          787 g          843 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc cggatgcgac
       61 actggcgcgt cttatccagc ctactcttgc ttatccgttc tgcttttgca tccactccac
      121 cacaaaatca gccagcccct caacatcatt aatatccagt aatgcgacat caagattaag
      181 cggcacatca ctggctacag caataacatg cctgtctatc actaattctt caggtcgatg
      241 tccggctcca tcgcgaaaca gcacaatctt tgcgatctct tcatgcttaa acccttcgac
      301 cagaatcaaa tccagctttg aggtatccat tcgacttgcg agaaactgta gatccagctc
      361 ttcttcgtct ggtttttccg tcatcaaggc ccatcgttgc tggctggcaa cgatggtttg
      421 tgccgcgcca gccttgcgca gctcatagct atctttgcct ggcttatcaa catccatatc
      481 atggtgcgta tgcttaatca gccctggacg gatccctctg gcgcataatg ccgggatcag
      541 ttttttcaaa agcgtagttt ttccggtacc actccacgcg gcaaaggcga gtaacggtat
      601 catcgttttt cctgccatcg ggcaagctcc tctggcgtat tcacgttaac aaatgcatct
      661 ttatgatcgc tgaaatcaac cgcatggccg ccagccagac gcataaatgc cattacccgg
      721 cgttctcctg cttgcagata ttctagtaat aaaggctcaa tagcacggtt taccagagca
      781 atagtcgggt gatcgcgttc accgtcatgg acccacacga caggcgcatc tttgcgctga
      841 tgattaagcc gggaagctaa aacatgggga atgtaaggcg tatcgcacgg acaaaacaaa
      901 aaccactcac cagcttcctg ctgcattact gaaagcattc ctgccagagg gcctgggtaa
      961 tccgccagtg aatcttcaat cactttcaga ccgcttgcct gatagatttc ctgatgacga
     1021 ttggcattaa ccacgacgtg agagagctgc gtcataagcg cgtcagcgac atgttgccat
     1081 aatggtttgc cgtttaattc aagcaatcct ttatctacgc cgcccattcg tctggcttta
     1141 ccgcctgcca gcacaacgcc tgttatcgtc gtcatcagat tcaccgatat cgcctctttt
     1201 attgtgggat tgaccctgct aacgtgtctg tctcaagagt aaggagcatc actatgaaat
     1261 gtaaacgtct gaatgaagtt attgaactcg tccagccagc ctggcaaaaa gagccagacc
     1321 ttaacctgtt gcaatttttg cagaaactgg cgaaagagtc aggttttgac ggcgaactgg
     1381 cggatttgac ggatgacatt ctgatctatc acctgaaaat gcgtgattcc gcgaaagatg
     1441 cggtgatccc gggtttgcag aaagattatg aagaagactt caaaacggcg ctgttacgcg
     1501 ctcgcggcgt aattaaagag taaaagcttg taagcggcgc caccaaaatc atcgtgaaat
     1561 gatatccttc atcattcgta atgttttccg gatgatggga tgaacaacag cgcttttact
     1621 ttccagacac tacacccgga taccatcatg gacgctctgt ttgagcaagg gatccgggtg
     1681 gattccggtc ttaccccgct taacagctat gaaaaccgtg tctatcaatt tcaggacgaa
     1741 gagcgtcgac gttttgtcgt caaattttat cgccctgaac gttggacagc cgatcaaatc
     1801 ctcgaagaac atcaatttgc gttgcagctg gtaaatgatg aagttccggt cgcagcacct
     1861 gtggccttta acggtcagac tttattgaat catcagggat tttatttcgc tgtttttcca
     1921 agcgtcggtg gtcgccagtt cgaagctgat aatatcgatc agacggaagc ggttgggcgt
     1981 tatttagggc gtatgcacca gacggggcac aaacagcttt ttatccatcg cccgaccatc
     2041 ggtctgaatg aatatctcat tgagccacgc aagctgtttg aggacgctac actgatacct
     2101 tccgggttga aagcggcatt cctgaaagcg acagatgagc tgatcgccgc cgttacagca
     2161 cactggcggg aagatttcac cgttctgcgg ctacatggag actgccacgc cgggaatatt
     2221 ctctggcgcg atggtccaat gtttgttgat ctggatgatg cacgtaatgg tccagctatt
     2281 caggatttgt ggatgttgct caatggcgat aaagccgagc agcggatgca actggaaact
     2341 attattgaag cttatgaaga atttggcgag ttcgacaccg ctgaaatcgg actgattgaa
     2401 cctttacgcg ccatgcgttt ggtttattat cttgcctggc taatgcggcg ttgggctgat
     2461 cccgcgttcc cgaaaaattt cccgtggtta accggggaag attactggct gcgacaaacg
     2521 gcgactttta tagaacaggc aaaagttcta caagaacccc ctttacaatt aacacctatg
     2581 tattaatcgg agagagtaga tcatgaaaaa gatttggctg gcgctggctg gtttagtttt
     2641 agcgtttagc gcatcggcgg cgcagtatga agatggtaaa cagtacacta ccctggaaaa
     2701 accggtagct ggcgcgccgc aagtgctgga gtttttctct ttcttctgtc cgcactgcta
     2761 tcagtttgaa gaagttctgc atatttctga taatgtgaag aaaaaactgc cggaaggcgt
     2821 gaagatgact aaataccacg tcaacttcat gggtggtgac ctgggcaaag atctgactca
     2881 ggcatgggct gtggcgatgg cgctgggcgt ggaagacaaa gtcactgttc cgctgtttga
     2941 aggcgtacag aaaacccaga ccattcgttc agcatctgat atccgcgatg tatttatcaa
     3001 cgcaggtatt aaaggtgaag agtacgacgc ggcgtggaac agcttcgtgg tcaaatctct
     3061 ggtcgctcag caggaaaaag ctgcagctga cgtgcaattg cgtggcgttc cggcgatgtt
     3121 tgttaacggt aaatatcagc tgaatccgca gggtatggat accagcaata tggatgtttt
     3181 tgttcagcag tatgctgata cagtgaaata tctgtccgag aaaaaataat tcaatgt
//
LOCUS       DAQSWQ010000223         3220 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.225, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000223 DAQSWQ010000000
VERSION     DAQSWQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.225"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            8..511
                     /locus_tag="R8G18_004290"
     CDS             8..511
                     /locus_tag="R8G18_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEE9797823.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(557..973)
                     /gene="rraB"
                     /locus_tag="R8G18_004291"
     CDS             complement(557..973)
                     /gene="rraB"
                     /locus_tag="R8G18_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEE9797824.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1135..2139
                     /gene="argF"
                     /locus_tag="R8G18_004292"
     CDS             1135..2139
                     /gene="argF"
                     /locus_tag="R8G18_004292"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEE9797825.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2211..>3220)
                     /locus_tag="R8G18_004293"
     CDS             complement(2211..>3220)
                     /locus_tag="R8G18_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEE9797826.1"
                     /translation="LLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDD
                     LNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPYI
                     NDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFF
                     NLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSIL
                     VNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGY
                     VVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGWN
                     N"
BASE COUNT          893 a          719 c          675 g          933 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
      481 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg
      541 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
      601 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
      661 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
      721 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
      781 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
      841 tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc ggatcgctgc
      901 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
      961 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     1021 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     1081 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg acctatgtcc
     1141 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
     1201 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     1261 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     1321 ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc aagcggcagc
     1381 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     1441 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     1501 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct ggcggatctc
     1561 ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct agtctatgca
     1621 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     1681 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
     1741 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga
     1801 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagag
     1861 aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa gatgatgcag
     1921 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     1981 acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     2041 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     2101 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     2161 cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt tcaattattc
     2221 caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt tcctaactgt
     2281 tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt catcattctt
     2341 gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac ataacccgga
     2401 agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc taactcatta
     2461 agatacagct gttcgccctc ataacactta agtaattgat agaagtacca cttagcaaaa
     2521 tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat gctgacaggc
     2581 acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg tgatttttct
     2641 tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat caattcctgt
     2701 aatttacggc tatttttata gggttcttta tcgagattga aaaataaggt ctcagctatt
     2761 ttaattttag attgatcgca gctaaaatct ttagattgat cgcagctaaa atcaatatga
     2821 tttatcacgg agcatgcata ctttatcttt tcagaatcac caaaatattg cattctttta
     2881 ttaaaacacc aatccagtat tcttacatca ttaatatatg gggattttaa aaaagtctcc
     2941 agaatcgaat cgatgaccgg tgtaaggtta acttttgcat catctaagga atcaataaga
     3001 atatgagaca ggttaacttt gatgtcatta atttcattgt cagtagcttt cttattatta
     3061 agaagataat tatttactgc gttaaggtca tctatttctt tcagtatccc tttttcacca
     3121 agcaagtatg aaatcgattc attacataca taaggcatta atagcagatg ttctttcctt
     3181 attgctaata atgcattgtt caatggtttt tctaataaat
//
LOCUS       DAQSWQ010000224         3218 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.226, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000224 DAQSWQ010000000
VERSION     DAQSWQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.226"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..495
                     /locus_tag="R8G18_004294"
     CDS             <1..495
                     /locus_tag="R8G18_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEE9797827.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="R8G18_004295"
     CDS             525..3041
                     /locus_tag="R8G18_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEE9797828.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3218
                     /locus_tag="R8G18_004296"
     CDS             3052..>3218
                     /locus_tag="R8G18_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9797829.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          938 a          771 c          731 g          778 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggagtt
//
LOCUS       DAQSWQ010000225         3195 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.227, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000225 DAQSWQ010000000
VERSION     DAQSWQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.227"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            84..3035
                     /locus_tag="R8G18_004297"
                     /pseudo
     CDS             84..3035
                     /locus_tag="R8G18_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3037..3195
                     /locus_tag="R8G18_004298"
                     /pseudo
     CDS             3037..3195
                     /locus_tag="R8G18_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          862 a          643 c          898 g          792 t
ORIGIN      
        1 taggtgggca ttgcacatat ttccgacgaa tagatcacaa tttatgctat tttgattttc
       61 acggttgcac atcaacagtt aatttgcgta aatcctatgg actgacaacg aaattcagta
      121 gattgacgct gggtgttttg ctggcactaa gcggttcagc gtctggtgcg agtctggaag
      181 ttgataatgg tcagattacc aatattgata ctgatgttgc ttatgatgcc tacctggttg
      241 gctggtatgg cactggagtt cttaatattt tggctgacgg taatgcctac ttaaccacta
      301 ttactaccag cgtcattggc gctaatgagg attcggaggg taccgttaat gttttgggtg
      361 gcacctggcg attgtatgat agcggaaata atgcaaggcc tttaaatgtg ggtcaatccg
      421 gaacggggac gctgaatatt aaacagaaag gtcacgttga tggaggctat ttaagactag
      481 gttcctcgac aggaggcgtc gggacggtca atgttgaggg agaagactct gttctgacga
      541 ccgaattatt cgaaataggg agttacggta cgggctcatt aaatattacg gataagggtt
      601 acgtcacgag ttcaatagtc gccattttag gatatcaagc gaacagtaat ggtaaggtta
      661 tcgttgaaaa gggtggcgag tggctaataa aaaataatga ttcctcaatt gaatttcaaa
      721 ttggtaatca aggagctggg gaggcgacta ttcgcgaggg tgggctgatt acggctgaaa
      781 atacgattat cggtggcaat gccaccggta tcggaaccct gaatgtgcag gatcaagact
      841 ctgtcatcac ggtacgcaga ctctataatg ggtatttcgg taatggcaca gtcaatattt
      901 ccaataatgg tctgattaat aacaaagaat attcattggt gggcgttcag gacggttccc
      961 acggtgtcgt caacgtgacc gataaagggc attggagttt cctcggaaca ggcgaagctt
     1021 tccgctatat ctatatcggt gatgctggtg acggtgaact taatgtctcg cgtgaaggta
     1081 aagtagattc gggaattatc actgcgggga tgaaagaaac aggcacaggc aacattactg
     1141 ttaaggataa gaactccgtt atcactaatc tcggaactaa tcttggttat gacggccacg
     1201 gcgaaatgaa tatcagtaat gaggggcttg ttgtcagcaa cggaggaagc tcactcggtt
     1261 atggagaaaa cggcgtcggg aatgtcagca tcaccacggg ggggatgtgg gaggtcaata
     1321 agaatgtcta taccaccatt ggtgttgcgg gcgtcggaaa cctcaatatt agcgatggcg
     1381 gtaagtttgt atcgcaaaat attacttttt tgggcgataa agcaagcggt atcggcacac
     1441 tgaacctgat ggatgcgaca tcatcgttcg atactgtggg tatcaatgtc ggtaattttg
     1501 gtagcggtat cgtaaatgtc agtaatggtg ccacccttaa ttcaacgggc tatggattta
     1561 tcggaggaaa tgcctccggt aaggggatag ttaatatttc aacggacagt ctctggaatt
     1621 taaagacatc atctactaac gcacaattgc tacaggtcgg tgtattaggc acgggtgaac
     1681 tgaatattac caccggaggt atagttaaag cgcgtgatac acagatagct ctcaatgaca
     1741 aaagtaaggg cgacgtgagg gtggatgggc agaactctct tcttgaaaca ttcaatatgt
     1801 acgtagggac atctggtacg ggtacgttaa ccctgacgaa tagcggcacg ctgaatgtcg
     1861 aaggtggaga agtttactta ggtgtttttg aacctgctgt aggaacgcta aacattggtg
     1921 cagctcacgg tgaggcggcg gcagatgccg ggtatataac caacgcgacg aaagtagagt
     1981 ttggttctgg cgaaggtgtt tttgtcttta atcatactaa taacagtgat gccggctacc
     2041 aggtcgatat gctgattaca ggtgacgata aagacggaaa agtgatccat gatgcaggcc
     2101 atacggtgtt caatgcaggg aatacttata gcggtaaaac gctggtcaat gacggcctcc
     2161 tgaccattgc gtctcatacg gcagatgggg taacgggcat gggttcgagt gaagtcacca
     2221 ttgcaagccc cggtacgctc gacattctcg catcaacgaa cagtgcagga gattacacgc
     2281 tgaccaatgc gctcaaaggc gatggcttga tgcgagtgca gctgtcatcc tacgacaaga
     2341 tgtttggctt tacccatgca acagggactg aattcgccgg tgttgcccaa ctgaaagaca
     2401 gaaccttcac tctggaacgc gacaacaccg ctgcgcttac tcacgcgatg ttgcagtctg
     2461 acagtgaaaa taccacatcg gtaaacgtgg gagagcaatc cattggtgga ctggccatga
     2521 atggcggtac gctcattttc gatacggata ttcctgctgc gacgcttgca gagggatata
     2581 tcagcgtcga tacgctggtt gtcggcgcgg gtgactacac ctggaaaggc cgtaactatc
     2641 aggtaaacgg gacgggcgac gtgcttatcg acgtgcctaa accgtggaat gatcccatag
     2701 cgaataaccc tctgacgacg ctcaatttgc tggaacacga tgatagccat gtcggcgttc
     2761 aactggtgaa ggcgcaaacg gttattgggt cgggtggctc attaacgtta cgtgatttac
     2821 agggcgacga ggtggaagcg gacaaaacgt tacacattgc gcaaaacgga acggtggtcg
     2881 ccgagggtga ttatggattc cgcctcacga ccgcaccagg tgatggtttg tacgttaact
     2941 atgggctgaa agcgctgaac atccatggtg ggcaaaagct gacgttagcc gaacatggcg
     3001 gagcctatgg cgcaacggcc gatatgtcgg caaaatagcc atcgcttaca cgaccgtctg
     3061 ggtgagccgc agtatacaga ttcactgcat tctcagggat cggcaagcag tatgtggatg
     3121 cgtcatgtcg gggggcacga acgtttcagg accggtgacg gccagctaaa tactcaggct
     3181 aaccgctatg tattg
//
LOCUS       DAQSWQ010000226         3107 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.228, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000226 DAQSWQ010000000
VERSION     DAQSWQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.228"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..1138
                     /locus_tag="R8G18_004299"
     CDS             <1..1138
                     /locus_tag="R8G18_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HEE9797830.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="R8G18_004300"
     CDS             1150..1599
                     /locus_tag="R8G18_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797831.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
     gene            1861..2997
                     /locus_tag="R8G18_004301"
                     /pseudo
     CDS             1861..2997
                     /locus_tag="R8G18_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          970 a          525 c          824 g          788 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttgctcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg gaaagatcat cagaagttca
     1741 ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc ttattaccta
     1801 aagctgtttt ttattgctta tacatgatca aatacttctt acataattaa ggagaacaaa
     1861 atggaactta aaaaattgat ggaacatatt tctattatcc ccgattacag acaagcctgg
     1921 aaagtggaac ataaattatc ggatattcta ctgttgacta tttgtgccgt tatttctggt
     1981 gcagagggtt gggaagatat agaggatttt ggggaaacac atctcgattt tttgaagcaa
     2041 tatggtgatt ttgaaaatgg tattcctgtt cacgatacca ttgccagagt tgtatcctgt
     2101 atcagtcctg caaaatttca cgagtgcttt attaactgaa tgcgtgactg ccattcttca
     2161 gatgataaag acgtcattgc aattgatgga aaaacgctcc ggcactctta tgacaagagt
     2221 cgccgcaggg gagcgattca tgtcattagt gcgttctcaa caatgcacag tctggtcatc
     2281 ggacagatca agacggatga gaaatctaat gagattacag ctatcccaga acttcttaac
     2341 atgctagata ttaaaggaaa aatcatcaca actgatgcga tgggttgcca gaaagatatt
     2401 gcagagaaga tacaaaaaca gggaggtgat tatttattcg cggtaaaagg aaaccagggg
     2461 cggctaaata aagcctttga ggaaaaattt ccgctgaaag aattaaataa tccagagcat
     2521 gacagttacg caatgagtga aaagagtcac ggcagagaag aaatccgtct tcatattgtt
     2581 tgcgatgtcc ctgatgaact tattgatttc acgtttgaat ggaaaggact gaagaaatta
     2641 tgcgtggcag tctcctttcg gtcaataata gcagaacaaa agaaagagcc agaaatgacg
     2701 gtcagatatt atatcagttc tgctgattta accgcagaaa agttcgccac agcaatccga
     2761 aaccactggc acgtggagaa taagctgcac tggcgtctgg acgtggtaat gaatgaagac
     2821 gactgcaaaa taagaagagg aaacgccgca gaattatttt cagggatacg gcacatcgct
     2881 attaatattt taacgaatga taaggtattc aaggcagggt taagacgtaa gatgcgaaaa
     2941 gcagccatgg atagaaacta tctcgcgtca gtccttgcgg ggagcgggct ttcgtaatct
     3001 ttccctaggg agtcagccga atgttgggaa ggatttgacg gatgaagaga agaactgctt
     3061 ggccggcggc agtacgggaa gcccgggagg atggggacca gaagatg
//
LOCUS       DAQSWQ010000227         3060 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.229, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000227 DAQSWQ010000000
VERSION     DAQSWQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.229"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..1231
                     /locus_tag="R8G18_004302"
     CDS             <1..1231
                     /locus_tag="R8G18_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEE9797832.1"
                     /translation="RQRFLRRYKAQIKQSISEAINKRSVTDVDSGESVSIPTEDISEP
                     MFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQGQASQDGEGQDEFVFQIS
                     KDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGVPANISVVRSLQNSLARRT
                     AMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAELRAKIERVPFIDTFDLRY
                     KNYEKQPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILLYLFLSRTYKNVEVVYIRH
                     HTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNPAQWNIYAAQASDGDNWAD
                     DSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHLQSTFDNFAMQHIRDQDDI
                     YPVFRELFHKQNATAKD"
     gene            1378..2853
                     /locus_tag="R8G18_004303"
     CDS             1378..2853
                     /locus_tag="R8G18_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEE9797833.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT          852 a          679 c          631 g          898 t
ORIGIN      
        1 tcgccagcgt tttttacgcc gttataaagc gcaaattaaa cagtcgatct ccgaggccat
       61 taataagcgt tcggtgactg acgtcgacag cggcgaatcc gtatccattc ccacggaaga
      121 tattagcgaa ccgatgtttc atcaggggcg tggcggcctg cgccaccgcg tacatccggg
      181 caatgaccat ttcgtccaga acgaccgaat tgaacgtccc cagggtggcg gcggaggttc
      241 cggcagtggt cagggccagg ccagccagga tggtgaaggt caggatgaat ttgtctttca
      301 gatttcgaaa gatgagtatc ttgatctgct ctttgaagat ttggccttac cgaatctgaa
      361 acaaaaccaa caacgccagc tgaccgaata taaaacgcat cgggcgggtt ataccgctaa
      421 cggcgttcca gccaatatca gcgttgtgcg ttccttgcag aactcactgg cgcgacgcac
      481 agccatgacg gcgggcaagc gacgggaact tcatgcgctg gaagagaatt tggccatcat
      541 cagcaacagt gaacctgcgc aactgctgga agaggaacgt ctgcgcaaag aaattgcaga
      601 attacgtgcc aaaattgaac gcgtcccttt tattgacacc ttcgatttac gttacaagaa
      661 ctacgagaag cagcccgatc cctccagcca ggcagtgatg ttttgcctga tggacgtttc
      721 cggttcaatg gatcaatcca ctaaagatat ggctaagcgt ttttatattc tgctgtatct
      781 gttcctcagc agaacgtata agaacgtgga agtcgtatac atccgccatc atacccaggc
      841 gaaagaagtc gatgaacatg agtttttcta ctcgcaggaa acaggcggca ccattgtttc
      901 cagcgccctg aaactgatgg atgaggtagt gaaagagcgt tataacccgg cacagtggaa
      961 tatttacgct gcacaagcat cggacggcga taactgggcc gatgactctc cgctttgcca
     1021 tgaaatcctg gcgaaaaaat tattacctgt tgttcgttat tacagctata tcgaaattac
     1081 ccgtcgtgca catcagacat tgtggcgaga atatgagcat ctgcaatcta ctttcgacaa
     1141 ctttgcgatg cagcacatcc gcgaccagga tgatatttat ccggtgttcc gtgaactgtt
     1201 tcataaacaa aatgcaacag ctaaagacta aaactatcag ccaggtcatt atcgcctggc
     1261 tgatttttag cttactgtaa attatctcat ttattacata cattagctta caatcgcttt
     1321 aaatatgaca gcataacctt tacataattt agttccagaa aacaatcatt cggaaaaatg
     1381 attcagtcaa cacgtatttc catggggtta ttctttaaat attttttatc gttaacgaaa
     1441 attgatcctg gtcaaaacta tatatctctg ccatcaataa aatccagcac tcacattgct
     1501 ctccttttta tggtttctat gggtacacaa aaattaaaag ctcaaagctt ttttattttc
     1561 agtttattgc tgacgttaat tttattttgc attactacct tatataacga aaacacaaat
     1621 gtaaaactca tcccacagat gaattacctg atggttgttg tggctttgtt tttccttaac
     1681 gccgtcattt ttcttttcat gttaatgaaa tatttcacta acaaacaaat tttaccaaca
     1741 ctcattttaa gccttgcatt tttaagtggc cttatctatt tagttgaaac cattgtaatt
     1801 atccataaac caattaacgg cagtacactg atccagacaa agtcgaatga tgtttctatt
     1861 ttctatattt tccgccaact cagttttatt tgtttaacct cgctggcact cttttgttat
     1921 ggaaaagaca acatccttga caacaataag aaaaaaacgg gaatcctgtt gctggcgctg
     1981 attccttttt tagtttttcc ccttctggca cacaatctga gcagttataa cgctgactat
     2041 tctttgtatg tcgtcgacta ctgtccggac aaccatactg cgacctgggg aatcaactat
     2101 acaaaaatat tggtttgtct gtgggcattt ttactgttct ttattatcat gcgtacacga
     2161 ttagccagcg aactatggcc gttaatagca ttattatgtc tggcatcgct atgctgcaac
     2221 ttacttctac tgactctgga tgagtataat tacaccatct ggtatatcag tcgcgggatt
     2281 gaagtttcca gtaaactgtt tgttgtgtct tttctgattt ataacatttt tcagaaactg
     2341 caactctcca gcaaactggc agttcatgat gtgctgacca atatttataa tcggcgctac
     2401 tttttcaaca gcgtagagtc attattgtcg agacctgttg ttaaggactt ctgtgtcatg
     2461 ctggttgata ttaatcagtt caaacgcatc aatgcccaat ggggacatcg tgtgggtgat
     2521 aaagtgctgg tttcaattgt cgatattatc cagcaaagca tccgccccga tgatatttta
     2581 gcgcgactgg agggtgaggt gtttggcttg ctatttaccg aactcaatag tgcccaggca
     2641 aaaatcattg cggaacgtat gcgtaaaaat gtcgaactcc tgaccggctt tagtaacaga
     2701 tatgatgttc ctgaacaaat gaccatcagt attggcacgg ttttttcaac gggtgacacg
     2761 cgtaatatct cgcttgtcat gacggaagca gataaagcct tacgcgaagc gaaaagcgag
     2821 gggggcaaca aagtgattat tcatcatatt taagtgcaaa aatattcaga gccatgcttt
     2881 ttgcgtggct tttgcataca atttattact attaccccta aatttcccct ccccagcggc
     2941 gcagagatga gtataattag cgcccctgtg ccaggccgca atcgaacttt atctggtttt
     3001 ctcgtttcac taaccgaagg agtgccattt atcatgaaat tgcaccatag aatgctccgg
//
LOCUS       DAQSWQ010000228         3045 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.230, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000228 DAQSWQ010000000
VERSION     DAQSWQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.230"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..172
                     /locus_tag="R8G18_004304"
     CDS             <1..172
                     /locus_tag="R8G18_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797834.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="R8G18_004305"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="R8G18_004305"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HEE9797835.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="R8G18_004306"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="R8G18_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="R8G18_004307"
     CDS             2190..>3045
                     /locus_tag="R8G18_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HEE9797836.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          710 c          836 g          804 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcactgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAQSWQ010000229         2992 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.231, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000229 DAQSWQ010000000
VERSION     DAQSWQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.231"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            18..1076
                     /locus_tag="R8G18_004308"
     CDS             18..1076
                     /locus_tag="R8G18_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEE9797837.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="R8G18_004309"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="R8G18_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HEE9797838.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="R8G18_004310"
     CDS             2805..>2992
                     /locus_tag="R8G18_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797839.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAQSWQ010000230         2936 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.232, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000230 DAQSWQ010000000
VERSION     DAQSWQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.232"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            35..262
                     /locus_tag="R8G18_004311"
     CDS             35..262
                     /locus_tag="R8G18_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HEE9797840.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="R8G18_004312"
     CDS             complement(263..637)
                     /locus_tag="R8G18_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HEE9797841.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="R8G18_004313"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="R8G18_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="R8G18_004314"
     CDS             complement(2097..2672)
                     /locus_tag="R8G18_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEE9797842.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          761 a          694 c          688 g          793 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctctgat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattac tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag gtttgttgtc tatagctata
     2761 gtgactcttc tatgtagata ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttc
//
LOCUS       DAQSWQ010000231         2917 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.233, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000231 DAQSWQ010000000
VERSION     DAQSWQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.233"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..493
                     /locus_tag="R8G18_004315"
     CDS             <1..493
                     /locus_tag="R8G18_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEE9797843.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="R8G18_004316"
     CDS             493..1083
                     /locus_tag="R8G18_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HEE9797844.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..2110
                     /locus_tag="R8G18_004317"
     CDS             1070..2110
                     /locus_tag="R8G18_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9797845.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2113..2658
                     /locus_tag="R8G18_004318"
                     /pseudo
     CDS             2113..2658
                     /locus_tag="R8G18_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2682..>2917
                     /locus_tag="R8G18_004319"
     CDS             2682..>2917
                     /locus_tag="R8G18_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HEE9797846.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          758 a          636 c          831 g          692 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacga
//
LOCUS       DAQSWQ010000232         2900 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.234, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000232 DAQSWQ010000000
VERSION     DAQSWQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.234"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1199)
                     /locus_tag="R8G18_004320"
     CDS             complement(<1..1199)
                     /locus_tag="R8G18_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HEE9797847.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="R8G18_004321"
     tRNA            1492..1586
                     /locus_tag="R8G18_004321"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1886..>2900
                     /locus_tag="R8G18_004322"
     CDS             1886..>2900
                     /locus_tag="R8G18_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEE9797848.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMST"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAQSWQ010000233         2890 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.235, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000233 DAQSWQ010000000
VERSION     DAQSWQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.235"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..905
                     /locus_tag="R8G18_004323"
                     /pseudo
     CDS             1..905
                     /locus_tag="R8G18_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
     gene            908..1441
                     /locus_tag="R8G18_004324"
     CDS             908..1441
                     /locus_tag="R8G18_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEE9797849.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1470..1997)
                     /locus_tag="R8G18_004325"
     CDS             complement(1470..1997)
                     /locus_tag="R8G18_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HEE9797850.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(1999..2841)
                     /locus_tag="R8G18_004326"
     CDS             complement(1999..2841)
                     /locus_tag="R8G18_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9797851.1"
                     /translation="MTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
BASE COUNT          812 a          637 c          621 g          820 t
ORIGIN      
        1 gcacaagcgg ggagaaaata ccgttactca gtacagcgaa tacctggact aatcgacaaa
       61 cattcagcgg tggcctttct ggtgaactat ccggcaatgc ttctacagct gcaaaattaa
      121 aaactgccag gaaaataagc aatgtggctt ttgatggttc ctccgatatc acattaaaag
      181 caagtcatgt tggtgcgttt gccttaggga aaacaggaag caccgttgcg aatgataaag
      241 cagttggatg gaactggagt agcggagcct ataacgcaac tattagtggt gcatcaacgt
      301 taattattca tttttatatg ggagaaggaa gttgtcctgc agctcagttt cggattaatt
      361 ataaaaatgg cggtattttt tatcgttcag cccgtgatgg ttatggtttt gaagccgact
      421 ggtccgaatt ttacaccacc agaaaacctt cagcaggaga tgttggtgca ctgccgttat
      481 ctggtggtca actgaatggt gcactgggta tcggaacatc cagtgctctt ggcggtaatt
      541 cgattgtatt gggtgataat gacacgggct ttaaacaaaa aggcgatggt aatctggatg
      601 tttatgctaa taacgtccat gttatgcgct ttgtctccgg aagcattcaa agtaataaaa
      661 ccataaatat tacgggacgt gttaatccct cggattacgg taactttgat tcccgctatg
      721 tgagagatgt cagacttggc acacgagttg tttagaccat gaagaaaggc gtgatgtatg
      781 aaaaatcagg ccatgtaatc acggggcttg gtattatcgg tgaagtcgat ggtgatgatc
      841 cggcagtatt cagaccaata caaaagttaa ttaacggaac atggtataac gtatcgcagg
      901 tataatcatg cagcatttaa aaaacattgt cgcaggtaat ccaaaaaccg ttgaacaata
      961 ccagctaaca aagaattttg atgttatctg gttatggtcc gaagatggaa aaaattggca
     1021 tgaggaatta aaaaactttc aggaggatac aataaaactg gcttacacag ttgagggaat
     1081 aattattgct atagacaagg atgtatcagc aattaatcca gaaggtttaa gtgtcgttga
     1141 gttgcctgat attacagcaa atcgccgggc tgatatttcg ggcaactgga tgttcaaaga
     1201 tggtgcggtg ataaagcgaa cttataccga ggaagagcaa aggcaacaag cggaaaatga
     1261 aaagcagagc ctgctgcagc tcgtcaggga taaaacccag ctgtgggact ctcagctaca
     1321 gctgggcatc atttccgatg agaataaaca aaaattaacc gagtggatgc tctatgcgca
     1381 gaaggttgaa tccacagaca cctccagcct gccagtaacg tttcccgaac aaccagaatg
     1441 agagaaggcc cgctatcggg ccttaatttt tattcaggct tttgtggcca ttcaggattt
     1501 gccgtatcca cacggctgac cagaacactg tagcgttccc atgcttccag tcgtgtgcgt
     1561 tcctcgtctg ttgccatatt cagcctgaca gcgcgttcca gcggctggat gactgattca
     1621 gcttcggaaa gcaatgcggc cttttgtgat tcggcctgtt gttgcagttc ctgttctgta
     1681 tattccctct ttactacttt accatccaga aacatccatt ttccgttatt gtctgcgtga
     1741 cgatttgccg taatatcagg aacttcaact atgcttagcc cttcaggatt aatagttgaa
     1801 acgtctttac tgacagcaac gatgatgttg ttctcatcgt aagcaatttt tatggtgtct
     1861 ggctgaaaat tcttttgttc ctcataccag tttttgccat cttcgcaaaa gagccatacc
     1921 acgcccgctt ttttagttag ctggtattgt tccggtgttt tcggattccc ggccttaata
     1981 tttttcaggt gcatcatctt aaacactccc cacgttgtac catattcccc caatcaattt
     2041 ctgaattggc cttctggcaa cagtgtcgac aatatcatcc gcattgctgt taattgcggc
     2101 tgttaaaaca taacctgacg catcgccaaa gccgtagtcg cgccatacgt ttttatattc
     2161 aatgctgcca agacgaatat cccgaacgac atgattatgc agccatgtgc ttaaccagtt
     2221 gttttcccac actgaaccat aaatatcgcc actagacgca acttgcgcac cacttcccag
     2281 cgcaaatccc ccatctgttt taaaaatgaa atcaccacca ccatctgaac cgttattaat
     2341 atgaacacca tcaccaccgt tatctttcca caaaaacata cgaattcttc cgtcttcgtt
     2401 ggcgaatgta atgtggtggc ggtaattact ttttaatcta aaattgctgc cattagattc
     2461 aatatcttta taaagatgta atcctaactc atcaatatgt ccgatgcgcg aaccatttga
     2521 atagaagcta ataataccat ctccatcttg cctaaatccg gtgtcgttat ctcccagaac
     2581 aatggagttt tcacccagtg cgttatcagt agctccaata gctagacctc ctttaatttt
     2641 ggccccgtgg ctgacagata tagcacctgt tctcagattt atagcgaatg gccttaatgg
     2701 gccgatatta ccgttttcgc cttgtccttc agccgtcgga atgaaatgaa gaaaatcttc
     2761 tgaacgacgg aaaattagac cgaaggtatc attgaaaatt cgcagcgcat tcaccgtgcc
     2821 aattttcagt tctccggtca ttttatcgcc ggaacgctga acggcgttac cagccttgtt
     2881 taccgtttcc
//
LOCUS       DAQSWQ010000234         2887 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.236, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000234 DAQSWQ010000000
VERSION     DAQSWQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.236"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            21..1103
                     /locus_tag="R8G18_004327"
     CDS             21..1103
                     /locus_tag="R8G18_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEE9797852.1"
                     /translation="MFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTFTAQKAVI
                     AIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKTL
                     RAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDALV
                     KGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLIR
                     PGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGKL
                     TSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYSD
                     HFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1563..2000)
                     /locus_tag="R8G18_004328"
     CDS             complement(1563..2000)
                     /locus_tag="R8G18_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797853.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2089..>2887)
                     /locus_tag="R8G18_004329"
     CDS             complement(2089..>2887)
                     /locus_tag="R8G18_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HEE9797854.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLKELIFSEPDSTLTVTD"
BASE COUNT          756 a          623 c          645 g          863 t
ORIGIN      
        1 tggcgtgggc ggtctttaaa atctttcgta ttccggtgaa tcagtggacg ctggcgacgg
       61 cggcgctggg aggcgtgttt ctggtgagtg gtttgatttt gttgatgaac tacaaccacc
      121 cttacacttt taccgcgcaa aaggcagtga tagcgatccc tatcacgcca caggtgacgg
      181 gaattgttac tgaagtcact gacaagaata atcagcttat tcaaaagggc gaggtgcttt
      241 ttaagctcga cccggttcgt taccaggcgc gagttgacag acttcaggct gacctgatga
      301 cggcgacgca taatataaag acgctgcgtg cgcagctcac tgaagcgcag gccaacacca
      361 cccaggtttc agcggagcgc gaccgtctgt ttaaaaatta tcaacgttac ctgaaaggca
      421 gccaggcggc ggtgaatccg ttctcggaac gtgacatcga cgatgcgcgg caaaatttcc
      481 tcgcgcagga tgcgctggtg aaaggctcgg tggcggagca ggcgcagatc cagagccagc
      541 tcgacagtat ggttaacggc gagcaatcgc agattgtgag cttaagagcg caacttactg
      601 aagcaaaata taaccttgag cagactgtca ttcgcgcgcc gagcaatggc tacgttactc
      661 aggtactgat ccgcccaggt acatacgcag ctgccttgcc gctgcgtccg gtgatggtct
      721 tcatccccga gcaaaaacgg caaattgtcg cccaatttcg gcaaaactcg ctgttacgtc
      781 tgaaacctgg cgatgatgcg gaagtggtgt ttaacgcgct acctgggcag gtgttccacg
      841 gcaaattgac tagtatttta cctgtcgtgc caggcggttc ttatcaggcg cagggggtat
      901 tgcaatcatt aacggtcgtt cccggcacgg acggcgtgct gggaaccatt gaactggacc
      961 ctaacgatga tatcgatgcc ttacccgacg gcatttacgc ccaggtagca gtttactccg
     1021 accatttcag ccatgtttcg gtgatgcgga aggtgctgct aagaatgacc agttggatgc
     1081 attatttgta tttggatcat tgagggggaa gggggagatt atggaacttt gctccctgca
     1141 agggctaatt atttttgcat attacttcat tggctgcgat gatttttctt taatattcat
     1201 tatgttaata agttctggat gagtgtgctc tcattggatt ttttatccgt cttgttctgc
     1261 cgcaggatga gactatacat cgtcgagatc gcaatgatga tatgagttgt tcctcctgcg
     1321 acggctctaa tcaaaaaaga gcaggagtgc ttttccatct attgcgatga tggctttatt
     1381 atctgaagga tgacagtcac atttaaaaaa tatttaatct atagatcaca atgtagggtc
     1441 atggaatata tgtatacaac tattttcttc ttcactttta attatcgtat tcattataaa
     1501 actaagttaa aaaaactcct agagagtgag ttattaatat atttttaaat aaaattaagt
     1561 tatcattcat cgatgcattt tatagaatca tcaatggcca taatagtact attaactctc
     1621 aattttatat ttttatcatc actacaaata ttaactgaat ttatatcaat gattttttta
     1681 ttcttatctt ttattttatt tactgtttca atgcaatcag cggcaagttg gagtggattt
     1741 agatttttca gatatggtct tatgatcttt cccttactta ataaatagca tgtataggat
     1801 tcggaaagtc cagcatctat tttgtacctc attatattag ataaggatgt gagaaattcc
     1861 ttgttatcat tcgcagccat atatgccagt ccgagttcag cctctgcggc cttacaggca
     1921 aaatagctta gtgaacacgc ttttcgtccc tctttagtac tggctgatat tagtgcccat
     1981 aatattgctt tgtctgccaa atctaactct ctctgaaatt gtcttgcagt aactgtactc
     2041 gcagaaatag agaaaaaaat acaattgtta ataactttgc catatttttt agtccgtaac
     2101 tgttaatgtg ctatctggct ctgaaaatat taactctttt aatttttgca tgtcattaga
     2161 tgaaccagtt atacaaccct cggaacagaa tcgaggtcct aaagacggac cgaaagcgtt
     2221 taaacaactt tgtgttaaaa atccatcacg attatatgtt tctgtatttg ctgacggtac
     2281 taaacgtata ccattaggtt ttctattggt tgctccagga ccattaacat tccagctcca
     2341 taccccttga ggtattggac ctctattttg taagtgtgta caaccaggat tatttttaca
     2401 ttgcatatta ttaccattat taccagaggc gacaggaata gtaagcacat cataagagtt
     2461 atcatcattt tgcgtatctg aaacgcatga aagcatactt aaagttatgc tgtataaaca
     2521 ggtcgctaat cccaacgggt caatatcagt tatcggattc aacggatact gataaaaatt
     2581 ccatcccccc ttcagtccaa tcggatcctg agtgatatat cgcccctgca gcgggtcata
     2641 atagcggtgg cggttgtaat acaggccgga ctcctcatca tactgctgcc ccggcaggcg
     2701 gataagctgc tgcagctgat gcgggttctc ttcattcagc aggttgcccc actcatcgta
     2761 ttctgcgcac cattctgttg ccccttccgt gctgataagc accagcggca ggccgcgatg
     2821 gtcgcagtgg tacaggtgga tttttcgcgc cggcgtgtac accgggtcca tctggttttg
     2881 catctgc
//
LOCUS       DAQSWQ010000235         2879 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.237, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000235 DAQSWQ010000000
VERSION     DAQSWQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.237"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="R8G18_004330"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="R8G18_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HEE9797855.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="R8G18_004331"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="R8G18_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HEE9797856.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="R8G18_004332"
     CDS             1650..2099
                     /locus_tag="R8G18_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEE9797857.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /locus_tag="R8G18_004333"
     CDS             complement(2100..2333)
                     /locus_tag="R8G18_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HEE9797858.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="R8G18_004334"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="R8G18_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HEE9797859.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2879
                     /gene="yncL"
                     /locus_tag="R8G18_004335"
     CDS             2787..>2879
                     /gene="yncL"
                     /locus_tag="R8G18_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HEE9797860.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          691 a          738 c          692 g          758 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggtc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttatt
//
LOCUS       DAQSWQ010000236         2797 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.238, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000236 DAQSWQ010000000
VERSION     DAQSWQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.238"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..617
                     /locus_tag="R8G18_004336"
                     /pseudo
     CDS             1..617
                     /locus_tag="R8G18_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            614..1093
                     /locus_tag="R8G18_004337"
     CDS             614..1093
                     /locus_tag="R8G18_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HEE9797861.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1147..2097)
                     /gene="corA"
                     /locus_tag="R8G18_004338"
     CDS             complement(1147..2097)
                     /gene="corA"
                     /locus_tag="R8G18_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEE9797862.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2467..>2797
                     /locus_tag="R8G18_004339"
     CDS             2467..>2797
                     /locus_tag="R8G18_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797863.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          842 a          620 c          591 g          744 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1081 tgactttaaa taagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1141 ctcatattac agccagttct tgcgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1201 cataaagata atcgcgcctg ggtagccgaa actccacttc agttctggca taaactcaaa
     1261 gttcatccca tagctggaag caacgagcgt cggcggcagg aataccacgg ataccaccga
     1321 gaagattttg atgatgcggt tctgctcgat gttgataaaa cccatcgccg cctgcatcag
     1381 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1441 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gctttacgca ccaggaagtt
     1501 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat cttccagttc
     1561 cgccagagtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1621 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1681 ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg actggctacg
     1741 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     1801 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     1861 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     1921 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     1981 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2041 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2101 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2161 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2221 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2281 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2341 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2401 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2461 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2521 atttttctcg ccctcacctt acttcccttg tttgctgttg ccgctgatga ttgcgcactc
     2581 tcagatccga tgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2641 atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct gctggtggat
     2701 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     2761 gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAQSWQ010000237         2739 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.239, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000237 DAQSWQ010000000
VERSION     DAQSWQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.239"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..337
                     /locus_tag="R8G18_004340"
     CDS             <1..337
                     /locus_tag="R8G18_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797864.1"
                     /translation="ELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVRVDEKERQ
                     VRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIERNAKARI
                     RRATKSRTE"
     gene            430..648
                     /locus_tag="R8G18_004341"
     CDS             430..648
                     /locus_tag="R8G18_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HEE9797865.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            650..1015
                     /locus_tag="R8G18_004342"
     CDS             650..1015
                     /locus_tag="R8G18_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEE9797866.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            1012..1677
                     /locus_tag="R8G18_004343"
     CDS             1012..1677
                     /locus_tag="R8G18_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797867.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1677..2042
                     /locus_tag="R8G18_004344"
     CDS             1677..2042
                     /locus_tag="R8G18_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HEE9797868.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2497..2652
                     /locus_tag="R8G18_004345"
     CDS             2497..2652
                     /locus_tag="R8G18_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HEE9797869.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          758 a          575 c          733 g          673 t
ORIGIN      
        1 tgagttaagc aatatcgttt tatttccggt aaaagaggat gacccacgta atcaggttaa
       61 ttttctttat gaaccatcgg aaagaccata ttgccatcac gcctctgtcc gggttgacga
      121 aaaagagcgt caggtccgct gtaaaatctg cggtgcggtt gtggagccgt ttgactggat
      181 gctctctgtg gcaaaaagag aaaccagact ggcagatgat gtaaggctat tgcgccagga
      241 ggaacaggaa aggcggagaa atatagaaaa gctgatacag attgagcgta acgcgaaagc
      301 gcggatacgc agggcgacaa aatccagaac tgaataatta aatttagcac tgttaaaaat
      361 ttaatcctta accggaggga tttctgcacc ctcaaatcat caggagaccg ctcgaaaggg
      421 cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag aacaacagaa
      481 ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct aatttttgtc
      541 tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt ggtatgggcg
      601 tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata tggcactgac
      661 gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt attgcggctg
      721 cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt ttgatggaaa
      781 tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc acccatgcaa
      841 tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg atggtcgtcg
      901 tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg ttgaaatgaa
      961 atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg catgaccact
     1021 attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga tattgacgat
     1081 atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact ggaagcagag
     1141 cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt cggtgagtat
     1201 gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg aatgcgacaa
     1261 cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca agttgaccag
     1321 gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg ttgggtacga
     1381 ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc gccggcagtg
     1441 ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc agattcatcg
     1501 cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt tgccaacgtc
     1561 actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag taatacgttt
     1621 gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga taaataatga
     1681 ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa tgtattgggc
     1741 aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc atgcgtaagg
     1801 ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca gttcctgctg
     1861 aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt tcctgggata
     1921 aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa gacggcgttg
     1981 aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag gtgaatcaat
     2041 gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc tggtggtgta
     2101 ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg gggtttttga
     2161 aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca agtaaacgta
     2221 tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat agatggtgct
     2281 ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg tgcttgaggc
     2341 tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt aacattacgc
     2401 gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa atgtaggtta
     2461 gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa ggcgatgtta
     2521 atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac gaggaaagac
     2581 ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt agactacgaa
     2641 tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg atgtgtcagg
     2701 catcctcaac gcacccgcac ttaacccgtt tcggcggtt
//
LOCUS       DAQSWQ010000238         2728 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.240, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000238 DAQSWQ010000000
VERSION     DAQSWQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.240"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(423..680)
                     /locus_tag="R8G18_004346"
     CDS             complement(423..680)
                     /locus_tag="R8G18_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797870.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="R8G18_004347"
     CDS             complement(677..1174)
                     /locus_tag="R8G18_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEE9797871.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="R8G18_004348"
     CDS             complement(1376..1834)
                     /locus_tag="R8G18_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HEE9797872.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="R8G18_004349"
     CDS             complement(1831..2328)
                     /locus_tag="R8G18_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEE9797873.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="R8G18_004350"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="R8G18_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HEE9797874.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          708 c          621 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataacca
//
LOCUS       DAQSWQ010000239         2702 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.241, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000239 DAQSWQ010000000
VERSION     DAQSWQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.241"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..246
                     /locus_tag="R8G18_004351"
     CDS             <1..246
                     /locus_tag="R8G18_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEE9797875.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(286..1494)
                     /locus_tag="R8G18_004352"
     CDS             complement(286..1494)
                     /locus_tag="R8G18_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEE9797876.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1502..2003
                     /gene="tnpA"
                     /locus_tag="R8G18_004353"
                     /pseudo
     CDS             1502..2003
                     /gene="tnpA"
                     /locus_tag="R8G18_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2021..2689)
                     /locus_tag="R8G18_004354"
                     /pseudo
     CDS             complement(2021..2689)
                     /locus_tag="R8G18_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          625 a          665 c          665 g          747 t
ORIGIN      
        1 gctgccctgc ccgtaagttg gatccagatg ctggcaggtc tggcgctgtt gagtaccatt
       61 ggcggcagtt tgtatcaggc gctgcataat gagcgtgagc gtgagcgcga cgcggcggtg
      121 gtggcatttc tggtaacggc aagtggattg acgctggtcg ggattggttc tgcgttctgg
      181 ggattaattg ccggaggcgt ttgttacgtg gtgttgaatt taatcgctga cagaaaccga
      241 tattgacatc ctccacgccc tgaaggacgt ggatccctgc tacgttcagg ctgtcgcctg
      301 aatcatttcg gtgggttcct gcttcaacgg gcggcctgac tgcaccatct ctccacaggc
      361 aagaacggcg tgccccgccg ctaaaatgtt acgagcacca tttacatcgg cgttcgctgt
      421 atatccacat acctggcatc tgaatttact ttgtgacagg cggttctctt tcgctgtatg
      481 accacagtac gcgcaacgct ggcttgtata cgctggcgga acagcaagca cctgaccgcc
      541 acgccagagc tgcttgtact caagctggcg gtgcatttca taccagccct gatccagtat
      601 cgaacggttt aaacctgatt ttgcccggac attgcgcccc ggctggctga ccgtacccgc
      661 tgctgacttt gacatgttgc tgaccttcaa atcctcaatg actatcattg cgtggttttt
      721 gctgacggcc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga tacaggaatg
      781 cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga ccttgcggct
      841 taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt ttacaggctc
      901 aaagactgtg ccatctgaca gcgtggcgag tttagccacg ccagcatcca gcccgaccat
      961 tgatgctgaa gggtgagccg gagtggatac ttcactttct gtctgaatac tgatgtacca
     1021 cgtaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct gacggctatt
     1081 ccggtagcgc atccagccca gtttcggcag aaaaatacgg ctgttttcct gatcgagctt
     1141 aacgccctgc gggtagcgga atgcatcatt ctgtccccgc tttttgaatc ggggaaaagc
     1201 agcccgcttc cggaagaagt ttttgtaagc ccgctcaagg tctttcagtg actgttgcaa
     1261 tggctgtgag ggagaatctt taagccattg cgtttcagtg gcatttttcc actcaaccag
     1321 ccaggaagcc attttcccgt aagggatgta tttattaccg gcctcatgat tctcattctg
     1381 acgtgccaga gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc tcatttcaca
     1441 ctcctgttga ccacctggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca
     1501 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
     1561 cctgatgcat gttcacctgg tctttgtcac tagataccga cgccagattt tttgacgcga
     1621 cagaaaaact acgcacttac ttttcaaatg tatgtgctta ttttgaagct gaactggttg
     1681 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga
     1741 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
     1801 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
     1861 gtagctgcgg aggtgtgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
     1921 ctggtcaggt ggaaaaccgc gccttatatc cccgccctga aggatggggt tttacggcgc
     1981 accggataaa aagcgccatc taacgatggc gcgtctggaa ctactttttg ttaacgtcaa
     2041 acatggtggc atcggtcgcc atgtcatcaa taacttgttt aatattttcg aaagccattg
     2101 gcgtactttc gttattcagg tctttacctt cgccctgacg aacgactttg ataaccggtt
     2161 tattagtcgc tgcatcaatc agctcacctt caaaatagag gcgagtatcc atggtacggt
     2221 ggcctgtagc catttgcgtc cccgcaacca ctaatgcaac aggaaccact tcatagaatt
     2281 gcagcccttc tttgctggta tctacaccgg taatggcccc acggaaaatc agactacgcg
     2341 gcccagcggt ggtaaccagt ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg
     2401 tatagttcaa aattttatcc agaactttct gccctaccta ggtagaaggt tttggcaccg
     2461 gataataagt gattgggttc cagacgatgc tgtcatattt gctttgatca aaactcgggt
     2521 ctacccaacg taaaacaggt ttacctgttg ctgaggttgt ttcttttaaa tcagagtaat
     2581 tgtttaaaaa accagaatat ttatctggct gggtgatttt agaggcacaa ccagacagag
     2641 ccaacaagcc gcataaagct gcaactttcg caaatgatgt ggtacgcatg aatagtttcc
     2701 tg
//
LOCUS       DAQSWQ010000240         2651 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.242, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000240 DAQSWQ010000000
VERSION     DAQSWQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.242"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(25..315)
                     /locus_tag="R8G18_004355"
     CDS             complement(25..315)
                     /locus_tag="R8G18_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEE9797877.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="R8G18_004356"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="R8G18_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HEE9797878.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="R8G18_004357"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="R8G18_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEE9797879.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="R8G18_004358"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="R8G18_004358"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2651)
                     /locus_tag="R8G18_004359"
     CDS             complement(2171..>2651)
                     /locus_tag="R8G18_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HEE9797880.1"
                     /translation="AVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAR
                     EARRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGD
                     ADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          698 a          718 c          600 g          635 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgaccattc cattcaaacg
     2461 tgaacggctt cgcctcctgt tctgtgcgcc atgcttcaat ttcctgacgt ctggcctctc
     2521 tggccgcttc cagcatttct ggtgtcacag tgaatggggc tatctcaccc catttgccac
     2581 tttgcagttc ctgccagatt tgctgacccg tcggtgcgac atcatcagcg gtggctgtgt
     2641 aggggactgc c
//
LOCUS       DAQSWQ010000241         2586 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.243, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000241 DAQSWQ010000000
VERSION     DAQSWQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.243"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            196..1149
                     /locus_tag="R8G18_004360"
     CDS             196..1149
                     /locus_tag="R8G18_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEE9797881.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="R8G18_004361"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="R8G18_004361"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEE9797882.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2445..2576)
                     /locus_tag="R8G18_004362"
     CDS             complement(2445..2576)
                     /locus_tag="R8G18_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HEE9797883.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
BASE COUNT          714 a          635 c          578 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatccc
//
LOCUS       DAQSWQ010000242         2556 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.244, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000242 DAQSWQ010000000
VERSION     DAQSWQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.244"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="R8G18_004363"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="R8G18_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HEE9797884.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="R8G18_004364"
     CDS             complement(390..1178)
                     /locus_tag="R8G18_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HEE9797885.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="R8G18_004365"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="R8G18_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEE9797886.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="R8G18_004366"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="R8G18_004366"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HEE9797887.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="R8G18_004367"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="R8G18_004367"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HEE9797888.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          580 g          664 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAQSWQ010000243         2547 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.245, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000243 DAQSWQ010000000
VERSION     DAQSWQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.245"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..159
                     /locus_tag="R8G18_004368"
     CDS             <1..159
                     /locus_tag="R8G18_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005087616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797889.1"
                     /translation="DAKRLQQLEPNAGTGARIWLAKEALESGDYDSEEAFYKAEGRTG
                     YSPGLGGV"
     gene            828..1673
                     /locus_tag="R8G18_004369"
     CDS             828..1673
                     /locus_tag="R8G18_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEE9797890.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1684..1872
                     /gene="dicB"
                     /locus_tag="R8G18_004370"
                     /gene_synonym="ftsT"
     CDS             1684..1872
                     /gene="dicB"
                     /locus_tag="R8G18_004370"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HEE9797891.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1869..2060
                     /locus_tag="R8G18_004371"
     CDS             1869..2060
                     /locus_tag="R8G18_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HEE9797892.1"
                     /translation="MNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKIPGD
                     CYPVDKVIHHDNNEIPAGL"
     gene            2145..2420
                     /locus_tag="R8G18_004372"
     CDS             2145..2420
                     /locus_tag="R8G18_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797893.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          744 a          557 c          599 g          647 t
ORIGIN      
        1 gatgcaaaac gcctccagca actggagcca aatgcaggca ctggggcacg catctggctg
       61 gctaaagaag cgctggaatc tggcgattat gatagcgaag aagccttcta caaagcagaa
      121 ggccgtacag gatattcacc gggtcttggc ggggtataaa taccatgcgc attgactgaa
      181 ttcgcaaaca aaaacagacg cgcgatacct ggatagtcgg tctgcatagt cagatatctg
      241 gctgcgaatt tgataaatat caccgccctt ttcggaaaca taagttccgt ccgggagttg
      301 attatacgaa ccatctccat gggggatcgt tctcctgaat cctagtgaga gcatatattt
      361 atgaagccct tcgtaatcct ctggctcagc attatatagt tctactctgg cgagatacgt
      421 tggcatattc atttccttac tggttgtgtg agaacttcag taaagatacc accaaagccc
      481 ggaggtggtg aaataaaacc gggcacaaca cgaaggcgca tttccggtat ccataaagag
      541 tcggtcttgt ctgttaaatt taaatggtgg gagtgcgcct ccggttgtaa ataacgacat
      601 tgctgtgtga agtaccagtt ggcggcatcg gtttaattgc tggctgatgt ccgccctttt
      661 taaagtgaat tttgtgatgc ggtgaatgcg gctcagcgca cgcggaacag ttaaaacagt
      721 aaggaaaatc ccttattccg gcgttaattg ttaactggtt aacgtcacct ggaggcacca
      781 ggcaccgcat caacaaagtt cacttcggtg atgaaaggaa agagaaaatg ttgaatgtag
      841 ctattgaaaa ccagaacggg tggaattata gtgcacctgc acctcataaa acgggtgccg
      901 ggattgctac cccgaagtca ttcacggcgc ataaccgcgc tcaggcggtt tttttatgcg
      961 taatgcacag ccacattcaa attatggtgg ggcgtgcggg gcagtcgcaa gactggccgg
     1021 gttccgtgat gaccggttgt agcaaccctg tacgtctcac cacccatgag attgctacct
     1081 ccggtggtga gttaatcaaa ttcatcgcgg aggctgccat catggctact accctttctc
     1141 acccttgtgt aaccgtagaa aacggtcgcg ctgtcactac gtctgttgcg gttgcagagt
     1201 tcttccacaa acgacacgac aatgtgttac gtgccatcgc aaatattgaa tgctcagata
     1261 aatttactgc cctcaatttt gaagcgagcg aatacaccga ctcaatcggg cgcaaactcc
     1321 caatgtacca aatcaccaaa aacggcttcg ttttcctggt tatgggcttc actggcaaaa
     1381 aagccgctgc attcaaagaa gcctacattg ctgagttcga ccgcatggag gccgaactgc
     1441 gccagaataa caccccacct gctgacaaga tgattccggg tgatggtcgc actctggttg
     1501 ttcacttcga caaattcggc aatgtcgaat tcaccgaaac cgttcctgat ggcgcactgg
     1561 tctgtaccct ggagactttc cggttttatc tggaaaaaca gggatggact cttgttaacc
     1621 ggggcgcaat taaaaatatg actgtggaac aattactcaa aattcattgt tgaggacgca
     1681 ataatggaga cgttattacc aaacgttaat acgtctgaag gttgttttga aattggtgtc
     1741 actatcagta accctgtatt tactgaagat gccattaaca agagaaaaca cgaacgggag
     1801 ctattaaata aaatatgcat tgtttcaatg ctggctcgtt tacgtctgat gccaaaagga
     1861 tgtgcacaat gaatacagta tttgcactcg ttctgacggt ttttcttgtt tccggtgaac
     1921 cggttgacat ggttactggc gtatacagct caatgaaaga atgtatgact gccgcagcga
     1981 aacagaaaat tcccggtgac tgttatccgg tcgataaagt tattcaccat gataataacg
     2041 aaatcccggc aggtctttaa aacagttccg taataaatat ccggtttcat tcttatatgc
     2101 cagcaatggc aggggttcgc tcaaccttaa ttaaggagaa aaacatgatt accagttatg
     2161 aagccactgt tgtaactact gatgacattg ttcacgaggt taatctggaa ggaaagcgta
     2221 ttggctacgt gattaagaca gaaaataaag aaaccccatt cactgtggtt gatatcgacg
     2281 gtccatcagg caacgttaaa acacttaacg atggtgtaaa aaaaatgtgt ctggtgcaca
     2341 taggaaagaa tctgcccgca gaaaaaaaag ccgaatttct ggcaactctg attgcaatga
     2401 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacac tgtgcaggcc tgagtgaaga
     2461 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
     2521 tgagcaccga taaacaagtt tacccac
//
LOCUS       DAQSWQ010000244         2541 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.246, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000244 DAQSWQ010000000
VERSION     DAQSWQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.246"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="R8G18_004373"
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="R8G18_004373"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEE9797894.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1522..2031)
                     /locus_tag="R8G18_004374"
     CDS             complement(1522..2031)
                     /locus_tag="R8G18_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEE9797895.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          642 a          660 c          580 g          659 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtccaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaagtcag ggcaagatta
     2461 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
     2521 catcttacgt cttaaccctg c
//
LOCUS       DAQSWQ010000245         2385 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.247, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000245 DAQSWQ010000000
VERSION     DAQSWQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.247"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(546..689)
                     /locus_tag="R8G18_004375"
                     /pseudo
     CDS             complement(546..689)
                     /locus_tag="R8G18_004375"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(693..1514)
                     /locus_tag="R8G18_004376"
     CDS             complement(693..1514)
                     /locus_tag="R8G18_004376"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEE9797896.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1503..2385
                     /locus_tag="R8G18_004377"
                     /pseudo
     CDS             1503..2385
                     /locus_tag="R8G18_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1783..1899
                     /locus_tag="R8G18_004377"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          620 a          528 c          563 g          674 t
ORIGIN      
        1 gctcattaaa aatatgacct tacgactgcg cctgcaggat cagcaggaag aaacatccgt
       61 gacattattt cagggaaaaa tgagtaccga cggaaataac tattacatca tcaaaggtaa
      121 agtaagctca gtacttctgt tattaaaaat gctgcagatt aatcacgcct gatctctgtc
      181 ccggtatttt agcccgaggt atattctgaa atatcattca tcatgaccgg cgtttataca
      241 aacgccggtc attcgctaac agcttcaccg ttccttcccc catccaggtt ccgcatgagc
      301 gtgttgtaat gttcataagc gtattatcag gattgtctgg aaaattcccg atactttgga
      361 ctattactcc tgaacaattc gaaattcagc agattaaagg taagcgttaa cggaggtaag
      421 cgttaacgga ggtaagcgtt aacggaggta agcgtaaacc gactgccgta tgtagccatt
      481 aagccagtat tggtaacgta ggtgcccacc ttttcaaatg atctgcgccc caaatcttgg
      541 acatcctatg aaaaatgttt aagtcgatat tgaaccggac tcatgccatc gagtttctct
      601 ttaatgcgat ggtgattgta gtattcaatg taatcaatta tatcttgctt tagatgatgt
      661 aacttattgt atttatttag gtagtaacat tcttatttta aagtactaaa aaagctttcc
      721 attgctgcat tatctaagca gttaccacgt cgggacatgc tttgtgttat gccattatca
      781 ctcaaccaac gttgccaacg aggcatctga tattgccacc cctgatcact atgcaacaag
      841 ggcttatcgt ctttattgag ttgttctacc gcatctaata gcatgctctt taccattgat
      901 gctaatgggc gctcagtaag gttatatgag actatttctc cattatacag gtccatgatt
      961 ggtgataaat agagcttcct gccatcgacc ttaaactctg tgacatctgt tacccacttt
     1021 tgatttgggc ggtcagcata aaattgtctc ttcagtaaat tattacaagt tcgcccctgt
     1081 tctccacgat aggcacggta cttcttgcgc ctgataagtg agctgatacc cagttccttc
     1141 attaactttc tgatggtttt accattaagt aaaatgccct cgttacggca ggcaagtctg
     1201 atgcgtcgat aaccataacg ccctttatgc tgatggtata ttctcaatat tacttgttta
     1261 acaagttgat atggatctgg cgcattaagg cgtttcacgt gatagtaaaa tgtactacgt
     1321 ggcaaatccg cgataaccag taattgtgat aatggatgac attgccttaa ctcattaata
     1381 atgattactt tttcttttgt tccaatcgta atttttcctg agctaaggct ttcagctttt
     1441 ttagataatg aaccgccccg ggaatcctgg agactaaact ccctgagaaa gaggtaaaca
     1501 ggatgactaa aaatactcgt tttcccccga agtccgtcaa cgggcagtcc gtatggttct
     1561 ggaaagtcag ggcgaatatg actcacaatg ggcggtaatt tgttccatta ctccaaagat
     1621 tggctgtaca ccggagactc tgcgtgtctg ggttcgccag catgagcggg ataccagggg
     1681 cggtgatgga gggctcacca ccgctgaacg tcagcgtctg aaagagctgg agcgtgagaa
     1741 tcgtgaactg cgctgcagta acgatatcct tcgccaggct tccgcttatt ttgcgaaggc
     1801 ggagttcgac cgcctctgga aaaaataatg ccactgctgg ataagctgcg tgggcagtac
     1861 ggggtcggac cggtatgcag tgaactgcat attgccccgt caacgtatta ccactgttag
     1921 caacaggaca tcatcctgat aaacgcagtg cccgtgcgca gcacgatgac tggctgaaga
     1981 gagagataca gcgcgtatac gatgaaaatc atcaggtgta cggtgtgcgt aaagtctggc
     2041 gccagttgtt acgggaagga atcagggtgg ccagatgtac agtggcgcgc ctcatggcgg
     2101 ttatgggact tgtcggtgtt ctccggggta aaaaggtccg cactaccgtc agccggaaaa
     2161 ccgttgccgc aggtgaccgc gtaaaccgtc agttcgtggc agaacgtcct gaccagctgt
     2221 gggtggctga ttttacttac gtcagcacat ggcagggctt cgtctatgtg gcgttcatca
     2281 ttgatgtgtt tgccggatac atcgtgtggt gacgagtctc atcgtctatg gaaacgacat
     2341 tcgtgctgga tgcactggag caggcgttgt gggcccgtcg tccgt
//
LOCUS       DAQSWQ010000246         2167 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.248, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000246 DAQSWQ010000000
VERSION     DAQSWQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.248"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(467..1018)
                     /locus_tag="R8G18_004378"
     CDS             complement(467..1018)
                     /locus_tag="R8G18_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEE9797897.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1817..2113
                     /locus_tag="R8G18_004379"
     CDS             1817..2113
                     /locus_tag="R8G18_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEE9797898.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          627 a          467 c          422 g          651 t
ORIGIN      
        1 ggcttactta gtggaaagag aggggatatg tgaccatagg ggaacccctc ctgttaaaag
       61 actacttaga agtctgaggt tttagtaaaa tctgtgaata tggcttctca gacgctctgt
      121 aacgcattct aagcgatttt acccctttat tttaaggtgg ttagtacatc cctataggtt
      181 acccctaaaa atggggttaa aactcgctac agagctttct atgagattag cttctaatcg
      241 aatcaactat gaggtttggt aggattgtgc aaaatctgta cagtaacttt tcagaatctg
      301 aaaattttta taaaaaatga ctagccctct ttgaatctca tcttccccga atcttcaaaa
      361 atatctgaca gatgtctgta gctgctaagt acccgtcaca ccagctatgt gacccccttg
      421 gcaaaaaagc caaatatcag ctacatccat gtaactattc agtaacttat ttagcaagtc
      481 tgtaataagt tgctctagag attcctaact cttcctgaat ctgcaacctc gtcttacctt
      541 gtgaaataag cgtctgtaca gcctctgaag aagcttttac aggcctcccc atatgtaccc
      601 cttcagcttt agcacgttca ataccagcct ttctacgctc tgctatcaag cttctttcaa
      661 gctgtgccaa cgttgacata atgcctaaca ttgcttcacc catagcgctt gataagtcaa
      721 acccttctct aaggctgata actgtcacac ctttagcctg tagcgcttta accgttgata
      781 acacatcaat tgtgtcacgt ccaagacggt ctacagctac cacaactacg gtgtcacctt
      841 ctcgtgcata agctaatagt ctgctgaaac cgtttcttaa agaagctttg acagaaccac
      901 tgacagcttc atcagtgaac catttagcaa tgttgtaccc tgcctcttca atttgcattt
      961 gctggttagt gatggattgg tcatctgtac tcaccctgca atatccaagt atcgccataa
     1021 tgttcccctt agaagtctca aaagttagtc tcataagttt agctgtctca taatccaaaa
     1081 acaacctttt gagactactt gagggtgcat tctaccgtta cgttgaaagt gtctcaaaag
     1141 gaagtccttt taagactggt tatgaaggag ctatgaggtt gattaggggt tggtacaagc
     1201 ccaactaacc gaatcaacta tgaagatttt aggagggtaa ctgtaaccat cactttctaa
     1261 ctttcacgta ggaaattgtt aagtggtttg atagttacac catgtgacca agcatctata
     1321 tatcgttata tcgccctgta tatagcacta tatgcagtgt cgagagagga acgactaaca
     1381 cgatacagtt taccctgaaa agagacttca cagattattt atatatttca ataagttagg
     1441 gtaaaaaggg actataccga gactgtatag aatctgtgta gaattgcata gatacttgga
     1501 aatctactat taaattgcat agggactcac ctgatattgt cctgttttta acaggtctcc
     1561 tagaaaaccg aatagacttc attcaagggt cattgaagtc ataatcattt cagtagaaaa
     1621 accaagtctc gattttaaat gcaaatagca atcatattca ataaggcaag acgatcagga
     1681 tcagtgaaaa tgtcattttt ggcgtaattg accttacttt atgcatggtc ttaacaaaga
     1741 catcatcttt ccgttgcaga tcttcgccct ttcaaataac gtactttaca actttcccga
     1801 acaaggagtt gtgcccgtgt tgtatgttat ttacgctcaa gacaaagctg atagcctcga
     1861 aaaacgcctt tccgttcgtc cggcacattt agcacgttta cagttactgc atgatgaagg
     1921 tcgtttgttg acagctggtc caatgccagc ggtagacagt aacgatccag gggctgcggg
     1981 atttactggc tcaaccgtta ttgctgaatt tgaatcgctg gaagcagctc aggcctgggc
     2041 cgatgctgac ccgtacgtag cggcaggtgt ctatgaacac gtttcagtga aaccatttaa
     2101 gaaagtgttc tgaatagaga ttcaatattt aagggcacgg tttttgcaac cgtgttcttg
     2161 tttggcc
//
LOCUS       DAQSWQ010000247         2089 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.249, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000247 DAQSWQ010000000
VERSION     DAQSWQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.249"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(60..1025)
                     /locus_tag="R8G18_004380"
     CDS             complement(60..1025)
                     /locus_tag="R8G18_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEE9797899.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDNGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="R8G18_004381"
     CDS             complement(1879..>2089)
                     /locus_tag="R8G18_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797900.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          436 g          527 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtt atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAQSWQ010000248         2005 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.250, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000248 DAQSWQ010000000
VERSION     DAQSWQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.250"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..389
                     /locus_tag="R8G18_004382"
     CDS             <1..389
                     /locus_tag="R8G18_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797901.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="R8G18_004383"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="R8G18_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HEE9797902.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="R8G18_004384"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="R8G18_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HEE9797903.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="R8G18_004385"
     tRNA            complement(1806..1881)
                     /locus_tag="R8G18_004385"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="R8G18_004386"
     tRNA            complement(1890..1966)
                     /locus_tag="R8G18_004386"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAQSWQ010000249         1979 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.251, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000249 DAQSWQ010000000
VERSION     DAQSWQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.251"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(358..1401)
                     /locus_tag="R8G18_004387"
     CDS             complement(358..1401)
                     /locus_tag="R8G18_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEE9797904.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1812..>1979)
                     /locus_tag="R8G18_004388"
     CDS             complement(1812..>1979)
                     /locus_tag="R8G18_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEE9797905.1"
                     /translation="LHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT          575 a          404 c          425 g          575 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccgaaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaataatat aataacaaaa
     1741 atcctccagc cttgtaggcc agaggataaa atacttaatt gaggaaaata tcagggaaaa
     1801 gtatttctga cttaactttc ccggctggag agtgaatcaa tgtaatcagc gtaccattgc
     1861 atcatttcac ggcggccatc gagatacagg gcatgattat aggtacccct gatggaattt
     1921 ttatccacat gagctaactg catttcaatc caggcggaat taaagccttg ttcatgcag
//
LOCUS       DAQSWQ010000250         1971 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.252, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000250 DAQSWQ010000000
VERSION     DAQSWQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.252"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..166
                     /locus_tag="R8G18_004389"
     CDS             <1..166
                     /locus_tag="R8G18_004389"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEE9797906.1"
                     /translation="SDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQL
                     IRTLEKFAKL"
     gene            complement(208..753)
                     /locus_tag="R8G18_004390"
                     /pseudo
     CDS             complement(208..753)
                     /locus_tag="R8G18_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            754..885
                     /locus_tag="R8G18_004391"
                     /pseudo
     CDS             754..885
                     /locus_tag="R8G18_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            1082..>1971
                     /gene="aldA"
                     /locus_tag="R8G18_004392"
     CDS             1082..>1971
                     /gene="aldA"
                     /locus_tag="R8G18_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEE9797907.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIIDS
                     RVINSGQVCNCAERVYVQKG"
BASE COUNT          482 a          506 c          478 g          505 t
ORIGIN      
        1 ttccgatatc attggcagcc atttcggttc ggtgtttgat gccacgcaaa cggaaattac
       61 cgccgtgggc gatttacaac tggtgaaaac ggtcgcctgg tacgataacg aatatggctt
      121 cgtcacgcag cttattcgca ccctcgaaaa attcgctaaa ctctgacgcg cacaggcgga
      181 ggaaaaaacc tccgcctctt ttactcatta cgactgtaaa taaaccacct gggtctgcag
      241 atattcatgc aggccatgtt taccatctgc gccgccaata ccggatttac gccatccggc
      301 gtggaagcct tgcatagctt cgaagttttc acggttgatg taagtttcac caaacttcag
      361 ccctttaatg gctttcatcg cgacgttcag attttgggta tagattgatg aggtcaggcc
      421 gtaatcactg tcattagcca ttgagatagc ctcttccagc gtgtcaaatg cgaccaccgg
      481 cagaaccggg ccaaaggttt cctcatgcat aatcgacatt tcctggcgaa catccagcag
      541 caatgtcggc ggataataat atcctttccc ctctaccgct ttgccaccca acgccactct
      601 cgccccttct tctactgcgc gcgccacttt ttgctcaacc ctttccagcg ccgcggcgtt
      661 aatcaacggc cccatcgcaa tgtcgttgcg ttcagcgggg ttaccaaatt taaccgcctg
      721 catcgcttca cccagccgat tgacgaactg atcaatggct acggtccccg cgggcgagat
      781 tttatcgttc acgttgattt tccggcagaa gtcatccatg catggcaaac gctgaaacat
      841 gatgcggtgc tcagcgaggc gatggaaagt cgctcgttac gttaaaaatt gcccgtttgt
      901 gaaccacttg tttgcaaacg ggcatgactc ctgactttta tttctgcctt ttattccttt
      961 tacacttgtt tttatgaagc ccttcacaga attgtccttt cccgatgccg tctctctgat
     1021 gattgatgtt aattaacaat atattcaccg aaaacaaaca tataaatcac aggagtcgcc
     1081 catgtcagta cccgttcaac atcctatgta tatcgatgga cagtttgtta cctggcgtgg
     1141 agacgcatgg attgatgtgg taaaccctgc tacagaggct gtcatttccc gtatacccga
     1201 tggtcaggcc gaggatgccc gtaaggcaat cgatgcagca gaacgtgcac aaccagaatg
     1261 ggaagcgttg cctgctattg aacgcgccag ttggttgcgc aaaatctccg ccgggatccg
     1321 cgaacgcgcc agtgaaatca gtgcgctgat tgttgaagaa gggggcaaga tccagcagct
     1381 ggctgaagtc gaagtggctt ttactgccga ctatatcgat tacatggcgg agtgggcacg
     1441 gcgttacgag ggcgagatta ttcaaagcga tcgtccagga gaaaatattc ttctgtttaa
     1501 acgtgcgctt ggtgtgacta ccggcattct gccgtggaac ttcccgttct tcctcattgc
     1561 ccgcaaaatg gctcccgctc ttttgaccgg taataccatc gtcattaaac ccagtgaatt
     1621 tacgccaaac aatgcgattg cattcgccaa aatcgtcgat gaaataggcc ttccgcgcgg
     1681 cgtgtttaac cttgtactgg ggcgtggtga aaccgttggg caagaactgg cgggtaaccc
     1741 aaaggtcgca atggtcagta tgacaggcag cgtctctgca ggtgagaaga tcatggcgac
     1801 tgcggcgaaa aacatcacca aagtgtgtct ggaattgggt ggtaaagcac cagctatcgt
     1861 aatggacgat gccgatcttg aactggcagt caaagccatc attgattcac gcgtcattaa
     1921 tagtgggcaa gtgtgtaact gtgcagaacg tgtttatgta cagaaaggca t
//
LOCUS       DAQSWQ010000251         1949 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.253, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000251 DAQSWQ010000000
VERSION     DAQSWQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.253"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..594)
                     /locus_tag="R8G18_004393"
     CDS             complement(<1..594)
                     /locus_tag="R8G18_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797908.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
     gene            complement(1024..1347)
                     /locus_tag="R8G18_004394"
     CDS             complement(1024..1347)
                     /locus_tag="R8G18_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797909.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            complement(1515..1934)
                     /locus_tag="R8G18_004395"
     CDS             complement(1515..1934)
                     /locus_tag="R8G18_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797910.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
BASE COUNT          576 a          405 c          345 g          623 t
ORIGIN      
        1 cagcttagcc agtcttgatt tcagcaaatt aatctcattt tctcttgctt tcatcagttt
       61 ctctttggca atttcttcaa gctcagcttg tgatttatca gcaacaatta gagacagtcg
      121 atgtggtgct ctgacagctc tttctgtacg aacaactctt tgaccttctg gtaaatctgc
      181 aatcacttta ccaatctcta cagggtcata cccccaaccg tcacggtcta gtcgagagaa
      241 acaagcataa gaattggttt gaagctccat cgaaataacc atttcgtcaa tttcgtcttc
      301 aagcttggac tgaattttct cgtaaacact tctcattgct tcttgagagt ctttaatagc
      361 ttggaaatat cggcgttgta gcttcaactt caaatcaagt tcacaccgtt cgttcataga
      421 aaaatcaatt tcaactcttg gaatctcgag tggttctggt ggaatatatg gacctttatt
      481 tacttcgtgt gattcccgcc aagctctatc aaaagattca cgcaaagtta attcgtgaat
      541 atcaagtgca cttggtctgt gttcgataag catttcatgt tcatttctaa tcattcgttc
      601 accccttagt ttatttgtta tttcaattta tagaagcaca ctcagcaatc agaggtgaag
      661 agtctgactg aacaagaacc tgccaagtgt gcttttaaaa actgaaaaag ccctaaacat
      721 ttctgtgaag ggcaaaggat tcaataatga atgaacaaga gtactttttg agatagcctc
      781 tcagactaac ctgaaaggcc atgacgaaaa gcattgcacc tttccaactc agaatatcca
      841 tccatcggat tattgaaaga aaaataagag agaattgctc tcccataatt aaaattatgc
      901 catgcttaag gttatttgtc aacagctttt taacaggttg ataacatcaa ttcttattta
      961 ccgcattgtt aataatgcaa ccaacaaata agccaagcaa cgtccaagca attattaaac
     1021 ccattagtga accctcacag aatccgtcac cagaatatta tcactgtaca ggattgtcac
     1081 agcacctttc aaaaattgct ctggggaaat actgtatatg ttcgtttgct cgataaccaa
     1141 tcttttcatg ttgctgtctg taattggtag catcattcga ggctcgttaa actccaccga
     1201 ctgaataaag tcagagatgt tttctgcatt atcagccatc agctttagta atttatcaaa
     1261 attaattgcg atacctgcat caatgaagct ttgcaccatc atagcagtgt cgttatcaac
     1321 cataatttgc attgggtaat tattcatttt taaatctcct atgaaacaca cctcagacct
     1381 cttaaaatag ccattctgag gccagtaacc atgcgggtta cagagggagt tgcactggct
     1441 ggtaaggtaa tttatcaaat taaggtgcaa ctctgcctgt aaagtgcatt ctaccgtttc
     1501 ttagatggtt atttttaatt gtctttatta atcaatgact taacgtagaa atactgtctc
     1561 cgtgaaatac catatttttc tgtaatgtat cgctgagatt gaccagcatt tatggcctga
     1621 gtgatatcat cacggttata gcggttacca cgggatttat atttgtcacc atccacatct
     1681 tctgaggtat tgaagtcttc aatcagctta attgcatcgt gaaggtcatc ttctgaggca
     1741 taatcgaagg tatggataat ctcaaaatca ccagtatctg ctggtgcatt cttccatctg
     1801 gagaagcggg attttaattg ctggtgtgtg aagggttcag cattacgagt taaatctttc
     1861 catgttccgt tattctcacg gacaaggtga cctctatggc gataaaacgc atgataatca
     1921 aatgtgcata gcattaaacc cccagctta
//
LOCUS       DAQSWQ010000252         1851 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.254, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000252 DAQSWQ010000000
VERSION     DAQSWQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.254"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..139)
                     /locus_tag="R8G18_004396"
     CDS             complement(<1..139)
                     /locus_tag="R8G18_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HEE9797911.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
     gene            892..1347
                     /gene="csgB"
                     /locus_tag="R8G18_004397"
     CDS             892..1347
                     /gene="csgB"
                     /locus_tag="R8G18_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HEE9797912.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1388..1840
                     /gene="csgA"
                     /locus_tag="R8G18_004398"
     CDS             1388..1840
                     /gene="csgA"
                     /locus_tag="R8G18_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HEE9797913.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          581 a          341 c          406 g          523 t
ORIGIN      
        1 gagaacgttg aatattatgt aattttcctg taattgccag cgattgttta aggtgctgca
       61 agagagccgt cgcctgcaaa gaaggtttag tgatcaacaa taatgtatga ccatgaatac
      121 tatggacttc attaaacatg atgaaacccc gcttttttta ttgatcgcac acctgacagc
      181 tgcctctaaa atagaagcac cagaagtact gacagatgtt gcactgctgt gtgtagtaat
      241 aaatcagccc taaatgggta aaatataaaa ctaatggatt acatctgatt tcaatctagc
      301 cattacaaat cttaaatcaa gtgttaaaca tgtaactaaa tgcaactcgt tatattaaaa
      361 tgttaatctt aaggttttat taagtttaga aatgatagaa aagttgtaca tttggttttt
      421 attgcacaat tttaaaaaat catacaaatg gtgataactt actaataatg catataaaaa
      481 atatttcggt gtagtccttt cgtcatgtaa aaagttgttg ttttttctcc acacctccgt
      541 ggacaaattt ttactccaaa aagacgaggt ttgtcacggc ttgtgcgcaa gacatatcgc
      601 agcaatcagc gatgggcaag aagaatgact gtctggtgat ttttgatagc ggaaacggag
      661 atttaaaaga aaacaaaata ttttttgcgt agataacagc gtatttacgt ggcttttaat
      721 actttggtat gaacaaaaaa agaaaaatac aacgcgcggg tgagttatta aaaatatttc
      781 cgcagacata ctttccatcg taacgcagcg ttaacaaaat acaggttgcg ttaacaacca
      841 agttgaaatg atttaatttc ttaaatgtac gaccaggtcc agggtgacaa catgaaaaac
      901 aaattgttat ttatgatgtt aacaatactg ggtgcgcctg ggattgcagc cgcagcaggt
      961 tatgatttag ctaattcaga atataacttc gcggtaaatg aattgagtaa gtcttcattt
     1021 aatcaggcag ccataattgg tcaagctggg actaataata gtactcagtt acggcaggga
     1081 ggctcaaaac ttttggcggt tgttgcgcaa gaaggtagta gcaaccgggc aaagattgac
     1141 cagacaggag attataacct tgcatatatt gatcaggcgg gcagtgccaa cgatgccagt
     1201 atttcgcaag gtgcttatgg taatactgcg atgattatcc agaaaggttc tggtaataaa
     1261 gcaaatatta cacagtatgg tactcaaaaa acggcaattg tagtgcagag acagtcgcaa
     1321 atggctattc gcgtgacaca acgttaattt ccattcgact tttaaatcaa tccgatgggg
     1381 gttttacatg aaacttttaa aagtagcagc aattgcagca atcgtattct ccggtagcgc
     1441 tctggcaggt gttgttcctc agtacggcgg cggtaaccat ggtggtggcg gtaataatag
     1501 cggcccaaat tctgagctga acatttacca gtacggtggc ggtaactctg cacttgctct
     1561 gcaaactgat gcccgtaact ctgacttgac tattacccag catggcggcg gtaatggtgc
     1621 agatgttggt cagggctcgg atgacagctc aatcgatctg acccaacgtg gcttcggtaa
     1681 cagcgctact cttgatcagt ggaacggcaa aaattctgaa atgacggtta aacagttcgg
     1741 tggtggcaac ggtgctgcag ttgaccagac tgcatctaac tcctccgtca acgtgactca
     1801 ggttggcttt ggtaacaacg cgaccgctca tcagtactaa tacatcattt g
//
LOCUS       DAQSWQ010000253         1815 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.255, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000253 DAQSWQ010000000
VERSION     DAQSWQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.255"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..1089
                     /gene="istA"
                     /locus_tag="R8G18_004399"
     CDS             <1..1089
                     /gene="istA"
                     /locus_tag="R8G18_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HEE9797914.1"
                     /translation="CSERTVRRYLKYPEPPARKTRHKMVKLKPFMDYIDMRLAENVWN
                     SEVIFAEIKAMGYTGGRSMLRYYIQPKRKMRPSKRTVRFETQPGYQLQHDWGEVEVEV
                     AGQRCKVNFAVNTLGFSRSFHVFAAPKQDAEHTYESLVRAFRYFGGCVKTVLVDNQKA
                     AVLKNNNGKVVFNSGFLLLADHYNFLPRACRPRRARTKGKVERMVKYLKENFFVRYRR
                     FDSFTHVNQQLEQWIADVADKRELRQFKETPEQRFALEQEHLQPLPDTDFDTSYFDIR
                     HVSWDSYIEVGGNRYSVPEALCGQPVSIRISLDDELRIYSNEKLVASHRLCSASSGWQ
                     TVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1089..>1815
                     /gene="istB"
                     /locus_tag="R8G18_004400"
     CDS             1089..>1815
                     /gene="istB"
                     /locus_tag="R8G18_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HEE9797915.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRK"
BASE COUNT          465 a          446 c          522 g          382 t
ORIGIN      
        1 tgctctgaac ggacggtcag acgctacctc aaataccctg aaccgccagc cagaaagacc
       61 cgccacaaaa tggttaagct gaaaccgttt atggattaca tcgacatgcg cctggcagag
      121 aatgtctgga atagcgaggt tatcttcgcg gagattaagg caatgggtta tacgggcgga
      181 cgttccatgc tgcgttacta catccagccc aaacgtaaaa tgcgtccgtc aaaaagaaca
      241 gttcgcttcg aaactcagcc tggataccag ctccagcatg actggggcga agttgaggtg
      301 gaggttgccg ggcaacggtg caaagttaac tttgcggtta atacgctggg gttctcccgc
      361 agcttccatg tcttcgccgc accaaaacag gatgctgagc atacctacga atcactggtt
      421 cgcgccttcc gctacttcgg tggttgtgtg aaaacggtgc tggttgataa ccagaaggct
      481 gcggtgctga agaataacaa cgggaaagtc gtgttcaact ccggattcct gttgctggct
      541 gaccactata acttcctgcc acgggcatgc cgtccacgca gggccagaac aaaaggtaag
      601 gttgagcgga tggtgaaata cctcaaggag aacttcttcg tccggtaccg caggttcgac
      661 agcttcactc atgtcaatca acaactggag caatggatag ccgatgtggc tgacaaacgg
      721 gagcttcgcc agttcaaaga aacgccggaa cagcgcttcg cgctggagca ggaacatctg
      781 cagccgttac cggatacaga cttcgatacc agttacttcg atatccgcca tgtgtcctgg
      841 gacagctata tcgaggttgg tggtaatcgt tacagcgttc ccgaagcgct gtgtggtcag
      901 ccggtatcga tacgaatatc gctggatgac gagttgcgga tctacagtaa tgagaaactg
      961 gtggcctcac atcgcctctg ttcagcatcg tctggctggc agacagtgcc ggagcatcac
     1021 gccccgctct ggcagcaggt cagtcaggtg gaacatcgac cactgagtgc ctatgaggag
     1081 ctgttgtgat gcatgaactg gaagtcctgc tgagtcgcct gaaaatggag catctgagtt
     1141 atcacgttga aagcctgctg gaacaggcag ctaaaaaaga gctgaactac cgggagttcc
     1201 tgtgcatggc gctacagcag gaatggaacg gcaggcatca gcgcggtatg gagtccaggc
     1261 tgaagcaggc tcgtctgccg tgggtcaaaa cgctggagca gttcgacttt accttccagc
     1321 cgggcatcga ccgtaaggtt gtccgggaac tggctggtct ggcgttcgtg gagcgcagcg
     1381 aaaacgtgat cctgctgggc cctcctggtg tcggaaaaac tcatctggcc atagctcttg
     1441 gcgtgaaagc ggtggatgcg ggacatcggg tactgtttat gccactggac agactgatcg
     1501 cgacactgat gaaagcgaaa caggaaaacc ggctggagcg tcagctacag caactgagtt
     1561 atgcccgggt gttgatcctg gatgaaatag gctatctgcc gatgaacaga gaggaagcca
     1621 gtctgttctt ccggctactg aaccgtcgat atgaaaaagc gagcatcata ctgacgtcaa
     1681 acaaagggtt cgcagactgg ggagaaatgt tcggagatca cgtactggca acagcgatcc
     1741 tggatcggct gctacatcac tcaaccacgc tgaatatcaa aggagagagt taccggttaa
     1801 aagagaaacg taaag
//
LOCUS       DAQSWQ010000254         1726 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.256, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000254 DAQSWQ010000000
VERSION     DAQSWQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.256"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..354)
                     /locus_tag="R8G18_004401"
     CDS             complement(<1..354)
                     /locus_tag="R8G18_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEE9797916.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(354..848)
                     /locus_tag="R8G18_004402"
     CDS             complement(354..848)
                     /locus_tag="R8G18_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HEE9797917.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1206..1382
                     /locus_tag="R8G18_004403"
     CDS             1206..1382
                     /locus_tag="R8G18_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797918.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1524..1676)
                     /locus_tag="R8G18_004404"
     CDS             complement(1524..1676)
                     /locus_tag="R8G18_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797919.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1664..>1726)
                     /locus_tag="R8G18_004405"
     CDS             complement(1664..>1726)
                     /locus_tag="R8G18_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HEE9797920.1"
                     /translation="IRDQTALRTLQEYIRTQCLR"
BASE COUNT          441 a          475 c          399 g          411 t
ORIGIN      
        1 ggcgagtcgt tttttggagt gttcgcgggc tttttcctcc gtcatctgaa taatcagcat
       61 atcagcagga tcgcaaatca cgttgtaaat cacccagccg tcaatcaggc cgatagtctt
      121 gccggttcgt gccgggccaa caaatatcac tgcgtcgtat tcacgcgagg ccaggcagtt
      181 catcggctca ataacatacg gtgccaccag cggatcccac gggactgagt tccctgcccc
      241 catgggcacc cgcatatact gagcaacggc atcagcaacc cgcattcgtc tcggtgcgcg
      301 aaggatataa cctgaatcgg ttcgtgctgc ctttgcggtt tcctgattca gcattactcc
      361 tcctgctgta attcctcctc atcatccgca cctgcttcag tcacccgcag ggctatctga
      421 tcgcgcagat catcaataat ggactgaaca cggctcacag cggcaggctg cagaccgcag
      481 tcacgttcaa gaatatccgg taatgtctcc agcacctgca cgaccgcttt tgcccagatg
      541 gcaaactccc gtctgacatc actggccgga atgagttgtg ccgtttcctg ttcgaactta
      601 agacgctcac gttcagactg ataccaggct ttgcgctcat gcgcgtccat ttcgccttct
      661 gcaaccggcg gtggtaatgc cagaaatgcc gacacaatat caaccacccg ataaagcttg
      721 aggttgcttt catgcccccc tgcaacgggt agattttgca gccttgccgc agcagtctgg
      781 cgatgtacac ctgacagtgc cgccagttga ctgatattca gcgtcagatt ttttaactct
      841 cgatccatac ccgctccaga atgttttaaa catgcatctt gcgaacaact ttaggcaaac
      901 ggtgttagtg atgaacaaaa aacaatcaaa atcgacacca taaaaataaa accactgtaa
      961 tatcaatcca ttacagtagt ggtgatgacg aatgaaattt caaaaactag cctttttccg
     1021 cgccgctccc gccccgtggc aggccacccc accgggagga cccgtcagcc tgacagccat
     1081 gacgaacgtc tgatacagcg ccctgcatga atgacatcgg gataatccag aaaggaatag
     1141 catcgtaccc acaagaatca gtgtaagtgt cctgtttctt ccacccccgc acaggactgg
     1201 cgagcatgag ggacaaaccc gcgaaccata aacgcggtaa aaacccggtg tgcatcgttt
     1261 ttgattattc ccgcacactc gcgcagaagg agttccccgt cgggctacgg tcatggttaa
     1321 tacgggaata cggcgacgat acagcgcatg atgtgtcagg cttgaatacc tttatccgtt
     1381 aaaagggata tcagttaagt tatcccgtgt agggtataag ccattatcaa agccactctg
     1441 taggaagtgg cttttgtaat ggcaataaaa agccccgcga atgcgaggct aaatcctggt
     1501 atttgtaatg actggctctt atctcaacgc agccccttac cgcgcgcaaa atgctcaata
     1561 tcaagcatca gcaatgagat gtttaatctg gattcactcc agaagtgagc accaccctgt
     1621 ctacagagcc agatgtgaag gatgatgagt aaaattatcg ctatcatcga aggcattgcg
     1681 tcctgatgta ttcctgaagc gttctcagtg ctgtttggtc gcggat
//
LOCUS       DAQSWQ010000255         1691 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.257, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000255 DAQSWQ010000000
VERSION     DAQSWQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.257"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(788..1459)
                     /gene="queE"
                     /locus_tag="R8G18_004406"
     CDS             complement(788..1459)
                     /gene="queE"
                     /locus_tag="R8G18_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEE9797921.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1598..>1691
                     /locus_tag="R8G18_004407"
     CDS             1598..>1691
                     /locus_tag="R8G18_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797922.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
BASE COUNT          553 a          344 c          303 g          491 t
ORIGIN      
        1 acgaatcgca ctctatcaat tttcacttaa agtatgaact ggatatcttt attcataaga
       61 actattcatc aacagcgtat agaggcggtt atgtaaaacc actcgttagc ctcaaaacaa
      121 agacaaattt ccagatttat tacttaatca atgaataaca agcttaaaaa acaaactatt
      181 aacctaaaga aaaaattaac agacattaat tcaatattaa aaaaataata gattaaaatt
      241 tcttaacgat ttaagaatca tacaaataac actttgatta aatttaattt tttgtatcga
      301 taattgtgaa cctctctggc atggagaact attttgaaca tgaggtgtta cgtggatatt
      361 ttgcttatta caagtactgc taatataaaa acttgagaaa gagataacgg gttatatggt
      421 ggtttatccc cgctggcgcg gggaacactc taaacataac ctattattaa ttaatgattt
      481 tttaagccag tcacaatcta ccaactttac agtatcacac aaacaacaca tccattatgt
      541 taaagagtac ttaattcatt gattaaaaag gtaaatattt aaaataactc tatacaaatt
      601 aaaatctacc aaactttacc gcaataattt tcactccagc gaaaaattaa tgccacagaa
      661 tttgtagaga atgctaatga ttagccctgg atgggtaaaa tggaggagtt ttcagaggcg
      721 acaaacaata taaatgagta gaagtcttta caggttccac ccatttataa cgcttataaa
      781 tgtttaatca ggcaatattt agatatttat gtgtttgcat cgacaaacgc caattacgcg
      841 caatgcaggt ttcaatgcac aaacgtgtgg catcatcctt ttggctaatc ggctgcagtg
      901 caatgactcg cggtttatca tcggtcagcg ttgccaatag ttcatccagt gcttcaatat
      961 cgcgtacgcg ccccactgga tgcttgattt cgttggctcg ctccagtgcc tgtgacaaca
     1021 cgtcatagcc gccgcgcatg ttcagctttg gcgatacggt aacccaggta ttcggtgtgc
     1081 agcgtacctc atgagtacca ctggtttcga tctggcagct aaaaccgttc ttttcgagca
     1141 gatcagtcag tggtagcaaa tcatgaatgc aaggctcacc acccgtaatc accacatgcc
     1201 gcgcggtgta tccctggcga ctaatgacag ccaacaagtc ttcactgctc gccgcccccc
     1261 acttatcact ctctttggtc ttcgccagaa tgctgaaaag ggagacttcc cgatcctcaa
     1321 gcttttccca ggtgtgtttg gtgtcgcacc aggcacagcc aaccgggcat ccctgtaaac
     1381 gaataaaaat ggcggggacg ccggtaaagt aaccctcacc ttgcagggtc tggaacatct
     1441 cgttaatcgg gtactgcata gcattctctg tgaagtggat aattgttaat tattgcagat
     1501 cctgccacaa caatcatgtc ttattaacat tctgttacag gcagatttaa agaaaaatgc
     1561 gaaacatatc gttaataatt aaagggagta acgtattatg tcagaagaaa ataaagaaaa
     1621 tggatttaat catgtcaaaa cattcaccaa aattatattt attttttctg tattagtttt
     1681 taatgataac g
//
LOCUS       DAQSWQ010000256         1629 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.258, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000256 DAQSWQ010000000
VERSION     DAQSWQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.258"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            104..292
                     /locus_tag="R8G18_004408"
     CDS             104..292
                     /locus_tag="R8G18_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HEE9797923.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            289..480
                     /locus_tag="R8G18_004409"
     CDS             289..480
                     /locus_tag="R8G18_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HEE9797924.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            574..>1629
                     /locus_tag="R8G18_004410"
     CDS             574..>1629
                     /locus_tag="R8G18_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEE9797925.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          361 c          391 g          379 t
ORIGIN      
        1 cggttttatc tggaagaaca gggatggact cttgttaacc ggggcgcaat taaaaatatg
       61 actgtagagc aattactcaa aattcattgt tgaggacgcg ataatggaaa cgttattacc
      121 aaacgttaat acgactgaag gttgttttga aattggtgtc actatcagta acacggtatt
      181 tactgaagat gccattaaca agagaaaaca cgaacgggag tcattaaata aaatatgcat
      241 tgtttcaatg ctggctcgtt tgcgtctgat gcaaaaagga tgctggcgat gaatacagca
      301 tttgcactcg ttctgaccgt tattcttaat acgggcgaac cagctgaact ggttaccgga
      361 gtatacaact ccatgaaaga atgcgtggct gctgcagctg aacagaaaat tcccggtaac
      421 tgttatccgg ttgataaaat cattcaccag gataataaag aagtcccggc aggtctttaa
      481 agcagccacg taataaatat ccggtttcat ttttatatgc cagcaatggc agggatttgt
      541 tcacccttaa atctgtaatg aggttaaaac aaaatgagta aagtctttat ttgcgccgcc
      601 attccggacg aaaaggcaac aaaagaagaa ggcgctaaac ctgtagccac tgccattgaa
      661 gccggcgacg aacgccgcgc ccgcgcaaaa tttcactggc aattcctgga acattatccg
      721 gctgctcagg actgcgctta taaatttatt gtctgcgagg ataaacctgg tataccccgc
      781 cctgccctcg attcctggga tgctgaatat atgcaggaaa accgctggga tgaggagtct
      841 gcttcctttg tcccggttga gactgaatcc gaaccgatga acgtcacttt tgacaagctg
      901 gcccctgaag tacagaacgc tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt
      961 gatatggtga ttagcgcgca ggaactgttg caggaagaca tggcaacatt cgacggacat
     1021 atcgttgaag cgttgatgaa aatgccagaa gttaacgcca tgtgtccgga gattaaactg
     1081 catgccatcg ggtggattaa gcataaatgt aagcctggtg caaaatggcc agaaattcag
     1141 gcagagatgc gcatctggaa aaaatgtcgc gaaggtgaac gcaaggaaac cggaaaatac
     1201 acgtctgttg ttgatctcgc ccgtgccaga gccaatcaac agcacactga aaattcaaca
     1261 ggaaaaatca gcccggccat tgctgccatt catcgcgaat acaagcagac atggaaaaca
     1321 ctggatgacg aactggccta cgctctctgg cctggtgatg tggatgccgg aaacattgac
     1381 ggcagcatcc atcgctgggc aaaaaatgaa gttatcgaca acgaccgcga agactggaag
     1441 cgtatctcgg catcaatgcg caaacagcct gatgcccttc gctacgaccg ccagactatt
     1501 tttggccttg tccgtgaacg tccgatcgac attcacaaag accctgtggc actgaacaaa
     1561 tacattactg aatacctgac tacaaagggc gtgtttgaag atgaaggaac aaatcagagc
     1621 gcaactgat
//
LOCUS       DAQSWQ010000257         1626 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.259, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000257 DAQSWQ010000000
VERSION     DAQSWQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.259"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            57..1529
                     /locus_tag="R8G18_004411"
                     /pseudo
     CDS             57..1529
                     /locus_tag="R8G18_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
BASE COUNT          469 a          422 c          390 g          345 t
ORIGIN      
        1 tccccggcag ccttcaggga aaaatatcat cagatggctg cttaaaaaaa gaacaaatgg
       61 tagtgtccgc tattgccagt acacctcagt attacgaagc aaaaaatgac aaagtaagaa
      121 aacgcctcgg tattaaaggc ggtttttact gggctgaagc gaaaaaatta tccattgcca
      181 tctcccgtgg tgctgttgcg attgacgatg ctggctacga tgaagatgac tttaaaaaac
      241 ctgttcgcgt caatttgccc gttgttggtg acctcccacc agaaggcgtg tttgatacgg
      301 aattctgcaa ccgttacgaa aaaggcgggg aagatggcat cacaatggta tttatcgcat
      361 cttctccctc tgttcaggac aaaccagcca gcactgacaa taccaacgtc aacggcgaag
      421 acatgacaga aattgaggag aacatgctcc tgccagtttc cggtcaggaa ctgcccattc
      481 gctggcttgc tcaacacggc agcgaaaaac cggtaacgca cgtttcacgc gacggactcc
      541 aggcattaca cattgctcgg gctgaagaac taccggctgt tactgccctg gctgtttccc
      601 acaaaaccag cctgctcgac ccgctggaaa ttcgcgatct ccacaaactg gttcgtgaca
      661 ctgacaaagt tttccctaat cctggtaatt caagcctggg actgataact gcttttttcg
      721 aagcatacct gaacgctgac tacaccgatc gaggactgct gacaaaagag tggatgaagg
      781 gtaatcgtgt ttcacacatc actcgcacgg cttccggtgc taatgctggc ggcggaaacc
      841 tcaccgatcg cggcgaaggt ttcgtacacg atctgacgtc actggcgcgc gacgtagcca
      901 ctggcgtact ggcccgttca atggatctgg acatctataa ccttcatccg gcacacgcta
      961 aacgcattga ggaaattatc gctgaaaata aaccgccctt ttctgttttc cgcgacaaat
     1021 tcatcaccat gcctggcggg ctggattatt cccgcgccat cgtggttgcg tccgtaaaag
     1081 aagcaccaat tgggatcgag gtcatccccg cgcacgtcac tgaatatctg aacaaggtac
     1141 tgactgaaac cgatcatgcc aaccctgatc cggaaatcgt ggatattgcc tgcggtcgct
     1201 cctctgcccc gatgccgcag cgtgtaacag aagaaggaaa acaggatgat gaagaaaaac
     1261 cgcaaccatc ttgcgcaatg gcagatgaac aggcaacggc tgaaacagtg gaaccggatg
     1321 caactgaaca tcatcaggac acgcagccgc tggatgctca gtcacaggta aatgctgttg
     1381 atgcgaaata tcagaaactg cggacagaac tccatgaagc ccggaaaaac attccgccca
     1441 aaaatcctgt cgatgcagac aaattactgg ctgcctctcg cggagaattt gttgaaggga
     1501 ttagcgaccc gaatgattcg aaatggattc ccggtcatca tatctcctca aatgaagtca
     1561 aaaaaacgga aaataaagtg cgacagactg aagataaaca gcaccagaac agcgaaccgg
     1621 aggagc
//
LOCUS       DAQSWQ010000258         1575 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.260, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000258 DAQSWQ010000000
VERSION     DAQSWQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.260"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            289..1404
                     /locus_tag="R8G18_004412"
                     /pseudo
     CDS             289..1404
                     /locus_tag="R8G18_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          623 a          286 c          221 g          445 t
ORIGIN      
        1 cacatctaaa acatcacaaa aaaaatcact tcacacacta tcagagaaca taataacttt
       61 ttaaaatgag cgcattttga tagttgttaa cgatttcctt tacgatacta tcactaccct
      121 ttttttatac acaaatatta ccgggtggag ataaaaggga aatcaaaggt aattatatca
      181 ggaagatata acgcattaca tttattgtgt gtagaagaaa atccattcct gttctaatta
      241 tccaatttaa acatcttagc ataaaacaaa tgatgaataa ggaattttat gtcaattgac
      301 tttaccccag gtataataaa tacatatcac ggcgatattt ataactgcac aacaaatacc
      361 gataatgcta aaacgccaga cacaccgaag tggccttgcg ataattggga agaacaacaa
      421 cccattaatt ccacattctc tggagaagga tatatctctg atcaatatga tttagtgcaa
      481 caccaacttc aacaaattaa tgcgtgccac acgaacacta catatacaaa tgcagactac
      541 tcaaaagttg tggctcaact tgtaagtctt attactaaca ttgaaacgat aagttcgaca
      601 caacttacgc aacagacaca atcaatactt aatcagatta ataacattag atatgaaaaa
      661 aacaaaagtg ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc
      721 ataactaaaa ttagcgtaga agagggaata cccataacat tcagtgtcca aaccatgttt
      781 tcagatacta attttattgc tgaacaacga gctgacttac caactaatat aaaagatatt
      841 caaagcctgt atcaaaaaat gacgaagtta tatattgagc acagtgaaaa taaaaacagg
      901 atgaaagtct ttgccggagc caactttatt gatttcaata tgacaggaca gaacctgtcc
      961 gggttcgttc taaccttgtc aaggttttat ttcgaagatc tacttaatat aaatttcact
     1021 gacgcaaatt taggtgacac tattttttac ataaagaaca ccctaccccc aaattatata
     1081 aagatggaca atatcttgac aaacaaatcg aaggtttatt ttcaacatta ttaaccatta
     1141 atgacaactt tttacgagca aaagcggaaa ttgcttcgac aataattaaa tttttagagg
     1201 ccaggataac caatctttca tacaatgaca tattaaaata tcagcaagaa ttccaaaaac
     1261 aatgttataa gcaagttaaa gcctttacga cactctctcg atacaataaa attcaaacat
     1321 gggcagaaat gtctgaatat cagttcgaag tatttcaata cgagacgctt aaccccaaaa
     1381 aaatgtcaca cacgccttat ttaaagaggc cacttcctaa tgaaaaagat ataaattatg
     1441 gggtggaaat agaaatacca tctggcaaaa gaattcgctt atcaaatcat tatcagaata
     1501 taataccata atattgatta ttagcacttt ataattattg aatgaaaata gattttatgt
     1561 aattttaaaa caaag
//
LOCUS       DAQSWQ010000259         1525 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.261, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000259 DAQSWQ010000000
VERSION     DAQSWQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.261"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            100..270
                     /locus_tag="R8G18_004413"
     CDS             100..270
                     /locus_tag="R8G18_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797926.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(397..954)
                     /locus_tag="R8G18_004414"
     CDS             complement(397..954)
                     /locus_tag="R8G18_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEE9797927.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(951..1226)
                     /locus_tag="R8G18_004415"
     CDS             complement(951..1226)
                     /locus_tag="R8G18_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HEE9797928.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1412..1525
                     /locus_tag="R8G18_004416"
                     /pseudo
     CDS             1412..1525
                     /locus_tag="R8G18_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          424 a          325 c          367 g          409 t
ORIGIN      
        1 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
       61 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      121 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      181 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      241 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      301 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      361 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      421 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      481 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      541 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      601 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      661 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      721 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      781 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
      841 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
      901 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
      961 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1021 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1081 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1141 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1201 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1261 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1321 caggcagtta aaaacacgga aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct
     1381 ttcagataca atgtcttatt gcatttttta aaacatgtca gtgtggtaca tggatatcga
     1441 taccatcgcc aattataaac tgggcggtgc acagcaacat gatagcgtac gcctcgatcc
     1501 gtgggtgttt atgttctcag cagga
//
LOCUS       DAQSWQ010000260         1514 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.262, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000260 DAQSWQ010000000
VERSION     DAQSWQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.262"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            8..>1514
                     /gene="sat"
                     /locus_tag="R8G18_004417"
     CDS             8..>1514
                     /gene="sat"
                     /locus_tag="R8G18_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HEE9797929.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGDVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVLLNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNS"
BASE COUNT          542 a          257 c          306 g          409 t
ORIGIN      
        1 agaatatatg aataaaatat actcccttaa atatagtgct gccactggcg gacttattgc
       61 tgtttctgaa ttagcgaaaa gagtttctgg taaaacaaac cgaaaacttg tagcaacaat
      121 gttgtctctg gctgttgcag gtacagtaaa tgcagcaaat attgatatat caaatgtatg
      181 ggcgagagac tatcttgatc ttgcacaaaa taaaggtatt ttccagcccg gagcaacaga
      241 cgtaacaatc actttaaaaa acggagataa attctctttc cataatctct caattccgga
      301 tttttctggt gcagcagcga gtggcgcagc taccgcaata ggaggttctt atagtgttac
      361 tgttgcacat aacaaaaaga accctcaggc cgcagaaact caggtttacg ctcagtcttc
      421 ttacaaggtt gttgacagaa gaaattccaa tgattttgag attcagaggt taaataaatt
      481 tgttgtggaa acagtaggtg ccaccccggc agagaccaac cctacaacat attctgatgc
      541 attagaacgc tacggtatag tcacttctga cggttcaaaa aaaatcatag gttttcgtgc
      601 tggctctgga ggaacatcat ttattaatgg tgaatccaaa atctcaacaa attcagcata
      661 tagccatgat ctgttaagtg ctagtctatt tgaggtcacc caatgggact catacggcat
      721 gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag acagcggctc
      781 tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa caacccatgg
      841 tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg ataaactggt
      901 tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata atgtaaccat
      961 taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta ctcaagaaaa
     1021 aataactaaa gacaaagata ttgtgttcac aaatggggga gatgtcctgt ttaaggataa
     1081 tttggatttt ggtagcggtg gtattatctt tgacgaaggc catgaatata acataaacgg
     1141 tcagggattt acatttaaag gagcaggaat tgatatcgga aaagaaagca ttgtaaactg
     1201 gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta ctctgaatgt
     1261 tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttcttc ttaatgaaga
     1321 aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac taaataaaga
     1381 taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg gtggcacgct
     1441 agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg atggaacaac
     1501 aataactaac tcag
//
LOCUS       DAQSWQ010000261         1481 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.263, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000261 DAQSWQ010000000
VERSION     DAQSWQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.263"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1011)
                     /gene="cadA"
                     /locus_tag="R8G18_004418"
     CDS             complement(<1..1011)
                     /gene="cadA"
                     /locus_tag="R8G18_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HEE9797930.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1091..1438)
                     /locus_tag="R8G18_004419"
     CDS             complement(1091..1438)
                     /locus_tag="R8G18_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEE9797931.1"
                     /translation="MTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          413 a          324 c          371 g          373 t
ORIGIN      
        1 ggtgtaaggc acccacgcgg agtcaaagtg gatggatttc acatccagtg ttttcttgat
       61 gaagtcggtg ttgtacagca gaccatcata ggtagagttg gtaattacag catgtaccgg
      121 ccaggttgcg tttggtgttt ctttcacgcg cttagcaatg gtagcgtgct ggaattcact
      181 ctgtgggata ccaccaagaa taccgtaagc gttacgggtc gggcggaaat agattggcgt
      241 aacatcgctc atcatcatca ggtgggtcag cgatttgtgg cagttacggt caatcagaat
      301 ggtgctgcct gccggagcag agtacatgcc aacaattttg ttcgcagtgg aagtaccgtt
      361 ggtcaccatg tagctgcggt ctgcgttaaa gacgcgagcg atatactgtt ctgcttcttt
      421 gtgtggacca ctatgatcca gcagagaacc cagttcagat actgaaatgg aaatatcaga
      481 tttcatggta ttcggaccaa agaaatcata gaacaggcta cctaccgggc ttttctggaa
      541 tgcagtaccg cccatgtgac caggagtaca gaaagtatat ttaccttcac gaacatattt
      601 aaacagtgct ttagtcagcg gaggcagaat agtgttgata tattcgtcag tggtctgctt
      661 gatcttatta gcaatatctt cagcagcacc cagcgcatat tcaaagaagc taatctgtaa
      721 acgcaggtca ttcaggctta catcgagagt ggaatacgta ttagcgaacg cgtacaacgg
      781 caggttctcg ttcattttgc taatttcttc gcacagctcg agattatatt tatcccagtc
      841 aaaaataacg ccgcacagac gcgcattgtt ttcgatcagt tttaataagt cgtcacggtc
      901 gttcgggtaa acaatctgga agttcagacg ttcaagcgcg cgatgaagtt cacggatggg
      961 ttcttcttta aaataaaccc ccatgtgatt caatattgca ataacgttca tagtcatatc
     1021 tccaggtaaa aaaggcccct cccaacacat gggacaaaat gaaaggagga gcctcggaaa
     1081 atacttttaa ttaatgtgcg ttagacgcgg tgtggttatc cattgagtgg ctctggcgct
     1141 cgtgcatttt gcgagcgtag aacatcagga taatcaggct gacgatgaag gtacctgcca
     1201 gctcgaagga gcttgcgccc atcagcgcga tgaagcagaa cacacaaccc agtacagagc
     1261 agatcaggct gacaaagttg cggatgttaa cgccttcaaa acgaatcagg tcaacgcaag
     1321 agtagaagta cggcagcata gtcagcagta ctgcgatacc ggtcagttca ccgaacaggt
     1381 cagatgcttt accaccggca gagttcatca gagtgatgag gatcatcagg gcagtcattt
     1441 tcactgcagc cagcagcagt aatgccgatc agttaaggat c
//
LOCUS       DAQSWQ010000262         1426 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.264, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000262 DAQSWQ010000000
VERSION     DAQSWQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.264"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..118)
                     /locus_tag="R8G18_004420"
     CDS             complement(<1..118)
                     /locus_tag="R8G18_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HEE9797932.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="R8G18_004421"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="R8G18_004421"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HEE9797933.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="R8G18_004422"
     CDS             complement(822..>1426)
                     /locus_tag="R8G18_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEE9797934.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          396 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggt gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAQSWQ010000263         1421 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.265, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000263 DAQSWQ010000000
VERSION     DAQSWQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.265"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>1421
                     /gene="rhsC"
                     /locus_tag="R8G18_004423"
     CDS             <1..>1421
                     /gene="rhsC"
                     /locus_tag="R8G18_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HEE9797935.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          422 c          436 g          225 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt a
//
LOCUS       DAQSWQ010000264         1392 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.266, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000264 DAQSWQ010000000
VERSION     DAQSWQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.266"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..459
                     /locus_tag="R8G18_004424"
     CDS             <1..459
                     /locus_tag="R8G18_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEE9797936.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="R8G18_004425"
     CDS             477..1115
                     /locus_tag="R8G18_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEE9797937.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAQSWQ010000265         1384 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.267, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000265 DAQSWQ010000000
VERSION     DAQSWQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.267"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1339)
                     /locus_tag="R8G18_004426"
     CDS             complement(<1..1339)
                     /locus_tag="R8G18_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEE9797938.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAQSWQ010000266         1378 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.268, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000266 DAQSWQ010000000
VERSION     DAQSWQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.268"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..1378
                     /gene="rhsB"
                     /locus_tag="R8G18_004427"
                     /pseudo
     CDS             1..1378
                     /gene="rhsB"
                     /locus_tag="R8G18_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
BASE COUNT          341 a          396 c          428 g          213 t
ORIGIN      
        1 cggcgaactg gcggtggtgt atgaccgtag tggcaaacag gtgcgcagct ttacttacga
       61 tgataaatac cggggccgga tggtggcgca ccgtcacacg ggccggccgg aaatccgtta
      121 ccgttacgac agcgacgggc gggtgacaga acagcttaac ccggcaggct taagctacac
      181 gtatcagtat gagaaggacc gcatcaccat caccgacagc ctgaaccgcc gtgaagtgct
      241 gcacacgtag ggtgaagccg ggctgaagcg ggtggtgaaa aaggaacacg cggacggcag
      301 cgtcacgcag agtcagtttg acgccgtggg caggctcagg acacagacgg atgccgcagg
      361 ccggacaaca gaatacagcc cggatgtggt gacgggcctc atcacgcgca tcaccacgcc
      421 ggatggcagg gcatcggcgt tttactataa ccaccacagc cagttaacgt cagccaccgg
      481 gcctgacggg ctggaaatgc gccgcaaata tgatgaatcc ggccgtctga ttcaggaaac
      541 cgcccctgac ggcgatatca cccgctaccg ttatgataat ccacacagtg acttaccctg
      601 cgcaacggaa gatgccaccg gcagccggaa aaccatgaca tggagccgtt acggtcagtt
      661 gctgaccgtc actgactgtt ccggttatgt aacccgttat gaccatgacc gttttggtca
      721 ggtgacggcg gtgcaccgcg aggaagggct gagccagtac cgcgcatacg acagccgtgg
      781 acagttaatt gcggtgaaag acacgcaggg ccatgaaatg cggtatgaat acaacgccgc
      841 cggtgacctg accgccgtca ttgccccgga cggcagcaga aacgggacac agtacgatgc
      901 gtggggaaaa gccatctgta ccacgcaggg cgggctgacg cgcagtatgg aatacgatgc
      961 tgccggacgg gtcatccgtc tgaccagtga aaacggcagc cacaccacct tccgttacga
     1021 tgtactcgac cggctgatac aggaaaccgg ctttgacggc cgcacacagc gttatcacca
     1081 cgacctgacc ggcaaactta tccgcagcga ggatgagggg ctggtcaccc actggcacta
     1141 tgacgaagca gaccgcctca cgcaccgcac cgtgaagggt gaaaccgcag agcggtggcg
     1201 gtacgacgaa cgcggctggc tgacagacat cagccatctc agcgaagggc accgggtgac
     1261 ggtgcattac gggtatgatg agaaaggccg gctgaccggt gagcgtcaga cggtgcatca
     1321 cccgcagacg gaagcactgc tctggcagca tgagaccaga catgcgtaca acgcgcag
//
LOCUS       DAQSWQ010000267         1376 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.269, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000267 DAQSWQ010000000
VERSION     DAQSWQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.269"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            3..983
                     /locus_tag="R8G18_004428"
     CDS             3..983
                     /locus_tag="R8G18_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEE9797939.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="R8G18_004429"
     CDS             998..>1376
                     /locus_tag="R8G18_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEE9797940.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacagtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAQSWQ010000268         1364 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.270, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000268 DAQSWQ010000000
VERSION     DAQSWQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.270"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..1259)
                     /locus_tag="R8G18_004430"
     CDS             complement(<1..1259)
                     /locus_tag="R8G18_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEE9797941.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          379 a          348 c          305 g          332 t
ORIGIN      
        1 gtgtaaccta ttgcgttgtt tgtattacgg tattcggcaa cgactttaat cattccctcc
       61 atcatacttg ccacttctgt ttcctgcggc gagatcatgc ggacattttt catgacctgg
      121 gattgcatca ccgtctggct accagagtct tccgggcgtt gccaggtctg gatctcctga
      181 tcgttaccgc caacagtacg ccagttggta attgcaccac tgaagatgtc acgcacctgt
      241 tgttcagtca gggaattaac cggattatcc gcattgacga tgaaaacaaa cgcttcacgg
      301 gcaaaaggcg tgtacagcaa agtaacgccc gattcctcag cgcgtttttt ctgcccaccg
      361 gaaggttgcg ccacaaaaat aatatcagca tcacctttaa caatcctgtt atacgcatcg
      421 ggggtacgag agctctccag atattccctg gtgtgaaaat cttccggtat aacgcttaaa
      481 gcataaaatg cagaggcata aattgggtac gccgccgtag caccatcgag tcgcggccag
      541 ttttgcgtaa actgaatttg cggtttacca cgcagtggtg tcagttgatt attgagttta
      601 tcgggtcgcc aggcccaggt atcaatattt tcgcgcacac cgacaccggg attgagctgt
      661 ttatataaat aaccctgata accagcgaca aaaccaagca tgagaaggat aaacaggagg
      721 gaattacggc aacgtagccc ggatgtaccg gttaaaggat gtctggttgg ccggccgtac
      781 cccagcgaaa aactgatatg cgcgccaacg ggtataatga ctatccacca gtactcacct
      841 ccgactactg agactaacaa aacgcccagc caggggagca taagatcagc caaccaaccc
      901 gatcccatct ggtgaaattg ataatctgcc agatgcagcg cgattatcca gccaaccagg
      961 attaataaac cgggtactaa aaaaagacat tgtcgccaga cggctatttc tcttttttct
     1021 ggtgcactat cgcctgccca tactcgcccc gagaaaaata gcattaatgc gcccagcgcc
     1081 agcataaaaa tgttgagcag aaggtaactt gttggcgtga taaggatatt acaaacttca
     1141 ataataccgg caagtccgac acccagcata gcaacaaccg ccgccaaaaa ttgcgtcagt
     1201 atggggatgc cgaaaaaagt cattaccagc gaggacaaaa tccattttct gttttgcatt
     1261 attctttcca ttctttttga atggtgaaat tatactcccc gagtcccctt gccccttctg
     1321 gacacttttc cgaaatgatg gcggaaaaaa acgggaccct ttgg
//
LOCUS       DAQSWQ010000269         1357 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.271, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000269 DAQSWQ010000000
VERSION     DAQSWQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.271"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..>1357)
                     /locus_tag="R8G18_004431"
     CDS             complement(<1..>1357)
                     /locus_tag="R8G18_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HEE9797942.1"
                     /translation="GITYANPVNPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYR
                     TRTPAPVGVFGPGWKAPFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLW
                     LARGGVAAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLT
                     VVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLS
                     SPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYT
                     AGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEG
                     LDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKA
                     QTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDR
                     QG"
BASE COUNT          236 a          490 c          382 g          249 t
ORIGIN      
        1 ccctgacggt catatttccg gctgctgcgt aaaccatccg gatatgtcac tgacgtcagt
       61 tgcaactggt tgttgtaacc ataccgtacc gtcctgccgt ccggcagtac cactgaagtc
      121 agctttccag acgccatatg cagccggtac tccgtccgcc gccccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcggct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcga
      481 acctgcgtcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaaacctgag aagaggaaac agaacgggga ctggccgggg aagataagga agaggcgcgc
      721 tgtttacggg acgcttccgc ccgctgcgcc tgcgtgctca gcgccatgtg aaaacggcgg
      781 cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacgt caccctccgc cgcccggtgg
      841 aaagtcagtg agcgcccgaa gccatccacc acgaccgtca gtacccggta tgcgggcggc
      901 tccggccagc tgagtatcca ccacggcccc tgaaggctgt ttgtcgccag gtacacatgc
      961 ggactcaggc gaacatcttc cggcagcacc tgccagagcg cgcttagcgg ctgcgaactg
     1021 tgctgcgccg ccactccgcc ccgcgccagc cagagtgact cgctgcggct gtagcttatc
     1081 tcgccgggaa acagcggctc aaagtgaatg ctcctgccgc cgctgtcgtt gagtatcagg
     1141 ccttcatcgc gtatctgtaa gcggatatcg aagggcgctt tccagccggg accaaacacc
     1201 cccaccggcg cgggtgttct ggtccggtag ctgctgtagg cacggaaaag aataaacggc
     1261 agcgggcagg gcagcgcaag gtccgtctcg cccggaagca ccttcgcacc cagcaccggg
     1321 ttcaccgggt tcaccgggtt agcataggta atccctc
//
LOCUS       DAQSWQ010000270         1345 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.272, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000270 DAQSWQ010000000
VERSION     DAQSWQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.272"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..>1345)
                     /locus_tag="R8G18_004432"
     CDS             complement(<1..>1345)
                     /locus_tag="R8G18_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HEE9797943.1"
                     /translation="GAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWK
                     APFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPL
                     SALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVV
                     DGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLVLTTQAQRAEEARKPHTASLSSPD
                     SPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGE
                     LRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYR
                     FEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDA
                     AGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKG"
BASE COUNT          218 a          504 c          380 g          243 t
ORIGIN      
        1 cccttctcat catactcccg gctgctgcga agcccgtccg ggtacgtcac cgacgtcacc
       61 tgccgctggc tgttatagcc ataccgtacc gtcctgccgt ccggcagtat cactgaagtc
      121 agctttccag acgccatatg cagtcggtac tccgtccgcc gtcccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcagct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcgc
      481 acctgcatcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaggaaggag ctgagagagg gcagggactg tcaggggaag ataacgaagc ggtgtgcggt
      721 ttacgggcct cttccgcccg ctgcgcctgt gtggtcagca ccaggtgaaa acggcggccc
      781 gcgccgtcca tcacccccgt caccgcgccc gccacgtcac cctccgctgc ccggtgaaag
      841 gccagcgtcc gcccgaagcc gtccaccacg cccgtcagca cccggtatgc gggcggctcc
      901 ggcggcagca cctcgtccgc ccccggcacc cgctccggcc agctgagtat ccaccacggc
      961 ccctgaaggc tgtttgtcgc caggtacaca tgcggactca ggcgaacatc ttccggcagc
     1021 acctgccaga gcgcgcttag cggctgcgaa ctgtgctgcg ccgctactcc gccccgcgcc
     1081 agccagagtg actcgctgcg gctgtagctt atctcgccgg gaaacagcgg ctcaaagtga
     1141 atgctcctgc cgccgctgtc gttgagtatc aggccttcat cgcgtatctg taagcggata
     1201 tcgaagggcg ctttccagcc gggaccaaac acccccaccg gcgcgggtgt tctggtccgg
     1261 tagctgctgt aggcgcggaa aagaataaac ggcagcgggc agggcagcgc aaggtccgtc
     1321 tcgcccggca gcaccttcgc accca
//
LOCUS       DAQSWQ010000271         1341 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.273, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000271 DAQSWQ010000000
VERSION     DAQSWQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.273"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..184
                     /locus_tag="R8G18_004433"
     CDS             <1..184
                     /locus_tag="R8G18_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797944.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="R8G18_004434"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="R8G18_004434"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEE9797945.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAQSWQ010000272         1320 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.274, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000272 DAQSWQ010000000
VERSION     DAQSWQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.274"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..348)
                     /locus_tag="R8G18_004435"
     CDS             complement(<1..348)
                     /locus_tag="R8G18_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797946.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAI"
     gene            complement(351..1061)
                     /locus_tag="R8G18_004436"
     CDS             complement(351..1061)
                     /locus_tag="R8G18_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797947.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1077..>1320)
                     /locus_tag="R8G18_004437"
     CDS             complement(1077..>1320)
                     /locus_tag="R8G18_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEE9797948.1"
                     /translation="RYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVTR
                     ENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT          351 a          317 c          293 g          359 t
ORIGIN      
        1 aattgcccgt tcatccgcac gccagttttc tttgttggca aggttgatac ggcgcagaga
       61 aaaaactacg tgaatattgt caaaatgccc ctgtgcaaca aattccagta aacgcggcag
      121 aaaatagcgg acctcatcgc tattttgcgg atcaggccct gggctactat tccaggcatt
      181 gattaaggaa tacgggatcg ctcgtaacga tgtactccgt aatgcctttt gttcctgctg
      241 actcaggcaa tattcacaac aaaccacaaa tttctgcggc acccgataac gacgaaaggt
      301 gtgataaatc tgctcaatta atgcctgcat ggcggggctg ggttgcatag ttaccccctc
      361 gcccgctgat cgcgtgtaaa cggttgatag acaaaacgag cattcgccgc gttcaggcga
      421 ataagggcgc tacgcgaaag ggaaaagctg gggcgaatgc aactattgca gtctcgacag
      481 ctttcctctg cgcaattttc cactgagtaa tgacgcgtgt agagtttcag ttcggcctgc
      541 ttatcgcgaa atccgcgtaa actgttattg ttatgaaatt tccaggcatc tttaaagacc
      601 tgaaagatat agacatcctc aatccaggag tcacattttt taagcgggcg ctttgctatt
      661 atcacccgct catccggatt tagcatcgcc gtaaattgta cggtgacgtc atttgtcgtg
      721 ttcaggtcac attgaacatc tggtacatca ctgatatagg ggtcaaatcc aaaatcagca
      781 ccacaggcac aaagacggct aatggtggcg gtatcgaggg aaaatcccgg ccataccttg
      841 ctggaggtta agccaatatt taatgtgaca tcatatcgaa gcaatccctc aagaccgtca
      901 gcaccgacga acttttcccc ttcatccaag gcctggttaa taagttgctg aacaatgcct
      961 tcatcccagt atgccgattt gcagttggca atcgtaatat ccacgcctgt gaagggatca
     1021 gtaccggcaa ggcttgcaaa taatgtatta actaattcca tttttattat ccagctttaa
     1081 ggtagcgtct tatacatcgg gacataacct acatcttcca ccaaactctg tccctgcggc
     1141 gttaaaaacc actccaacaa tttttgtgtc tctgacgtcg tattttcccg cgttaccata
     1201 aaggcatcga cgatataggg atatttgccg ttgcgaatgt tttcggcagt cggtgcaata
     1261 ccgttaatcg ctagcagttt tatattttta tcggcgttca tttgtgttgc gtaataacgg
//
LOCUS       DAQSWQ010000273         1299 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.275, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000273 DAQSWQ010000000
VERSION     DAQSWQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.275"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..1117
                     /locus_tag="R8G18_004438"
     CDS             <1..1117
                     /locus_tag="R8G18_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HEE9797949.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
BASE COUNT          356 a          263 c          400 g          280 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaag
//
LOCUS       DAQSWQ010000274         1234 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.276, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000274 DAQSWQ010000000
VERSION     DAQSWQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.276"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..761)
                     /locus_tag="R8G18_004439"
     CDS             complement(<1..761)
                     /locus_tag="R8G18_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEE9797950.1"
                     /translation="MLNVAIENQNGWNYSAPAPYKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     DAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPTDKI
                     IPGDGRTLVVHFDERGNIRFTETVPDGARVCTMET"
BASE COUNT          307 a          299 c          310 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcgagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aacg
//
LOCUS       DAQSWQ010000275         1211 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.277, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000275 DAQSWQ010000000
VERSION     DAQSWQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.277"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..680)
                     /locus_tag="R8G18_004440"
     CDS             complement(<1..680)
                     /locus_tag="R8G18_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HEE9797951.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="R8G18_004441"
     CDS             complement(996..>1211)
                     /locus_tag="R8G18_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEE9797952.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAQSWQ010000276         1208 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.278, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000276 DAQSWQ010000000
VERSION     DAQSWQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.278"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..>1208)
                     /locus_tag="R8G18_004442"
     CDS             complement(<1..>1208)
                     /locus_tag="R8G18_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HEE9797953.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQW
                     QYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYS
                     EQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYE
                     LATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHT
                     TAANLDIDIP"
BASE COUNT          182 a          415 c          340 g          271 t
ORIGIN      
        1 tacgggatat cgatatccag gttcgctgcc gtggtgtgga cgcccgtcag cctccccgtg
       61 tcgctgtaac ggtactcccg gctctcctgc gggccactga tgcggattag ctgtccgttg
      121 tcgttccagt cgtagtcacg gtcaagctgc ggaaggttca ggtgcctgct ccggagctgg
      181 ccgctggtat tccaggcggt ggcaagttca tatgcctccc cgccgaagct gcgggccgtc
      241 tcacggtgca gccggtcccg cgtgtactcg accagcggtg ttccgcccag cttcatcccc
      301 gcaagataac cgctgccata cgtcagccac tccaccggcg gcaggccgtc cggctcctga
      361 cgggtcgcca gcccctgttc gctgtacgcg tgccccgtct catgctccca cagcatctcc
      421 cccgtctccg gggtctccac cgtctgccgt tcgcccgtca gacggccttt atcgtcatag
      481 ccgtagtgga ccgccacacg gtggccttca ctcaggtggc tgatttctgt cagccagccg
      541 tggtcgtcat actgccactg ctccgccggt tcaccgttca ccgtgcgccg ggtgatacgg
      601 tccgacgcat cgtaatgcca gagggtgata agcccctcat cttcactctg cgtgagtttt
      661 ccggtcaggt catactggta acgttgcgtc cggccgtcaa agccgcgctg ctccgtcagc
      721 ctgtcgaccg ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacagtg
      781 atgcgcccgg cggcgtcata ccccatgctt cgcgtcagac cgccctgcgt ggtgctgacg
      841 gcctttcccc acgcatcata ctgtatctca ctgcggctgc cgtccggggc aacgatagcg
      901 gtgaggtctc ctgcggcgct gtactcataa cgggtttcac gcccctgcgc atccctctga
      961 ctgaccagtt gaccgcgcgg gttataactg ctgtaagtgc tgatgccttc ttcccggtga
     1021 acggcgattt gttgaccgta ccggtcatac tcataccgcg tcgtgtaccc cgagcagtcc
     1081 gtaaacgcct gaagctgacc gtaccggttt cgcgttattc tccgcctcac cccggttccg
     1141 tcttccaccg cacagggcag gccactgcgg gaagaatcat aaaaccagcg cgtaatgtta
     1201 ccgttgcg
//
LOCUS       DAQSWQ010000277         1176 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.279, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000277 DAQSWQ010000000
VERSION     DAQSWQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.279"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..118
                     /gene="rcbA"
                     /locus_tag="R8G18_004443"
     CDS             <1..118
                     /gene="rcbA"
                     /locus_tag="R8G18_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HEE9797954.1"
                     /translation="KLTKSECEKMLSGEKEAGVSREQKVKLINFNCEKLQSS"
     gene            218..433
                     /gene="xisR"
                     /locus_tag="R8G18_004444"
     CDS             218..433
                     /gene="xisR"
                     /locus_tag="R8G18_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HEE9797955.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            435..>1176
                     /locus_tag="R8G18_004445"
     CDS             435..>1176
                     /locus_tag="R8G18_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HEE9797956.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEK"
BASE COUNT          406 a          213 c          270 g          287 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgtttc
       61 cagagagcag aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat
      121 tgcattgtat tcaaattaaa acttcatagc tgattattaa taatcaacat cgggcgtcaa
      181 tttcagccta acattggcgc ctgccagagg tgatgcgatg gcacaagtaa tctttaatga
      241 agagtggatg gttgaatacg gcctgatgct tcgcactggt ctgggtgcca gacaaattga
      301 agcataccgc cagaactgtt gggtggaagg cttccacttc aaacgagtat ctcctttagg
      361 gaagccagac agtaagcgag ggattatctg gtacaactat ccgaagataa atcagtttat
      421 caaagactca tgatatgtct aaattaccaa caggtgtcga gattcgaggt aaatacattc
      481 gcatctggtt catgtttcga ggaaaacgat gtcgggaaac attgaaaggc tgggagatta
      541 ctaacagtaa cattaaaaag gccgggaatt taagagcgtt gatagttcat gaaatcagtt
      601 ccggtgaatt tgagtattta agacgttttc cccagtccag cactggggca aaaatggtga
      661 caacgagggt cataaaaaca ttcggggagc tttgtgatat ctggacaaaa attaaagaga
      721 cagagttaac aacaaacaca atgaagaaaa cgaaatcaca attaaaaaca ctcaggataa
      781 taatttgtga gagtaccccg atatcgcata ttcgttatag cgatatctta aactaccgga
      841 atgaactgct gcatggagaa acgctttacc tagataatcc aagatccaac aaaaaaggaa
      901 gaaccgtgcg cacagttgat aactatatcg ccctgctctg ttcgttgtta cgttttgcgt
      961 atcagtcggg atttatatca accaaaccat ttgaaggagt aaaaaaatta cagcgaaaca
     1021 gaataaagcc tgacccgtta tctaaaacag aattcaatgc attaatggaa agtgaaaaag
     1081 gacagagcca aaacttgtgg aaatttgccg tttactccgg gcttcgtcac ggggaactgg
     1141 cagctctggc gtgggaggat gtggatttcg agaagg
//
LOCUS       DAQSWQ010000278         1170 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.280, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000278 DAQSWQ010000000
VERSION     DAQSWQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.280"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>1170
                     /gene="fimD"
                     /locus_tag="R8G18_004446"
     CDS             <1..>1170
                     /gene="fimD"
                     /locus_tag="R8G18_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HEE9797957.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQM"
BASE COUNT          331 a          261 c          283 g          295 t
ORIGIN      
        1 agccattatg catatttaaa cctacagagt gggctaaata ttggtgcgtg gcgtttacgc
       61 gacaatacca cctggagtta taacagtagc gacagttcat caggtagcaa aaataaatgg
      121 cagcatatca atacctggct tgagcgagac ataattccgt tacgttcccg gctgacgctg
      181 ggtgatggtt atacccaggg cgatattttc gatggtatta actttcgcgg cgcacaattg
      241 gcctcagatg acaatatgtt acccgatagc caaagaggat ttgccccggt gatccacggt
      301 attgctcgtg gtactgcaca ggtcactatt aaacaaaatg ggtatgacat ttataatagt
      361 acggtgccgc cggggccttt taccatcaac gatatctatg ccgcaggtaa tagtggtgac
      421 ttgcaggtaa cgattaaaga ggctgacggc agcacgcaga tctttaccgt accctattcg
      481 tcagtcccgc ttttgcaacg tgaagggcat actcgttatt ccattacggc aggagaatac
      541 cgtagtggaa atgcgcagca ggaaaaaacc cgctttttcc agagtacatt actccacggc
      601 cttccggctg gctggacaat atatggtgga acgcaactgg cggatcgtta tcgtgcgttt
      661 aatttcggta tcgggaaaaa catgggggca ctgggcgctc tgtctgtgga tatgacgcag
      721 gctaattcca cacttcccga tgacagtcag catgacggac aatcggtgcg ttttctctat
      781 aacaaatcgc tcaatgagtc aggcacgaat attcagttag tgggttaccg ttattcgacc
      841 agtggatatt ttaatttcgc tgatacaaca tacagtcgaa tgaatggcta caacatcgaa
      901 acacaggatg gtgttattca ggttaagccg aaattcaccg actattacaa tctcgcttat
      961 aacaaacgcg ggaaattaca actcaccgtt actcagcaac tcgggcgcac atcaacactg
     1021 tatttgagtg gtagccatca aacttattgg ggaacgagta atgtcgatga gcaattccag
     1081 gctggtttaa atactgcatt cgaagatatc aactggacgc tcagctatag cctgacgaaa
     1141 aacgcctggc aaaaaggacg ggatcagatg
//
LOCUS       DAQSWQ010000279         1157 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.281, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000279 DAQSWQ010000000
VERSION     DAQSWQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.281"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            6..407
                     /locus_tag="R8G18_004447"
                     /pseudo
     CDS             6..407
                     /locus_tag="R8G18_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="R8G18_004448"
     CDS             572..>1157
                     /locus_tag="R8G18_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEE9797958.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAQSWQ010000280         1145 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.282, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000280 DAQSWQ010000000
VERSION     DAQSWQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.282"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..187
                     /locus_tag="R8G18_004449"
     CDS             <1..187
                     /locus_tag="R8G18_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEE9797959.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="R8G18_004450"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="R8G18_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEE9797960.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          273 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAQSWQ010000281         1115 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.283, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000281 DAQSWQ010000000
VERSION     DAQSWQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.283"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            83..1114
                     /locus_tag="R8G18_004451"
     CDS             83..1114
                     /locus_tag="R8G18_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HEE9797961.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          300 g          227 t
ORIGIN      
        1 agatcacttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc aaatcaaaac
       61 aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg acgaacgacg
      121 cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca gactgactgc
      181 catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc tctgctgcgc
      241 ccgttcctct gttggacgct ggattaactg gttcacgcag tcgggtgttg agggactgaa
      301 atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca cactgttacg
      361 tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct ggagtacaga
      421 actgctggca ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg
      481 ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg
      541 tgacccgcat aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc
      601 agagcatccg gtcttttatg aagatgaagt ggatatccat cttaatccca aaatcggtgc
      661 ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata
      721 ttatctggcc ggagcgctgc acagcgggac aggtaaagtc agctgtgtgg gcggcaacag
      781 caaaagttcg gcgctgttca tcagcctgct gaagcggctt aaagcgacat accgtcgggc
      841 gaaaaccatc acgctgatcg tggacaacta cattatccac aaaagccggg aaacacagag
      901 ctggctgaag gagaacccga agttcagggt catttatcag ccggtttact cgccatgggt
      961 gaatcatgtt gaacggctat ggcaggcact tcacgacaca ataacgcgta atcatcagtg
     1021 cagctcaatg tggcaactgt tgaaaaaagt tcgccatttt atggaaaccg tcagcccatt
     1081 ccccggaggc aaacatgggc tggcaaaagt gtagc
//
LOCUS       DAQSWQ010000282         1088 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.284, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000282 DAQSWQ010000000
VERSION     DAQSWQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.284"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="R8G18_004452"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="R8G18_004452"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEE9797962.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAQSWQ010000283         1087 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.285, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000283 DAQSWQ010000000
VERSION     DAQSWQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.285"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            528..>1087
                     /gene="fimB"
                     /locus_tag="R8G18_004453"
     CDS             528..>1087
                     /gene="fimB"
                     /locus_tag="R8G18_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HEE9797963.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          333 a          189 c          233 g          332 t
ORIGIN      
        1 ccaggataaa aaggaatgta acaatctcat ggcgtaagct gacgaaccag gcaggaataa
       61 tcgctaggga cctaagcatt agcatgataa tagccactaa gaaattactg tgttccatga
      121 aatagccatt ttgtggcaaa tggagttgac taataatgtc atatgtgaga cggctagttg
      181 aacgaatatt aaattttgct gaatttttta tgttgatttt acttgttaca gaacatatca
      241 catgatatat agataagatt agttgcatta atgatgaggg ttattattag attcgtatca
      301 ggttgataaa tatataaagg tacatagcat gcaagagcat ggcgtttgta tggcaaagtt
      361 attataagta actatcgcta ctccatttaa gttcgctcag aagaactggt ccacttacgt
      421 tagttattaa gcaaacgttc gcttttataa acataatcag gattaaaatg ttggattatt
      481 gctaacccag cacagctagt gcgcgtctgt aattataagg gaaaacgatg aagaataagg
      541 ctgataacaa aaaaaggaac ttccttaccc atagtgaaat cgagtcactc cttaaagcag
      601 caaataccgg gcctcatgca gcacgtaatt attgtctgac tttgctttgt tttattcatg
      661 gtttccgggc gagtgaaatt tgtcgattga ggatttcgga tattgatctt aaggcaaagt
      721 gtatatatat ccatcgatta aaaaaaggct tttcaacaac gcacccgcta ttgaacaaag
      781 aagttcaggc tttaaaaaac tggttgagta tccgtacttc ttatccgcat gctgagagcg
      841 agtgggtatt tttatcacgt aaggggaatc cgctttctcg gcaacagttt taccatatta
      901 tctcgacttc cggtggtaat gccgggttgt cactggagat tcatccgcac atgttacgcc
      961 attcgtgtgg ttttgctttg gcgaatatgg gaatagatac gcgacttatc caggattatc
     1021 ttgggcatcg caatattcgt catactgtct ggtataccac cagcaatgca gggcgttttt
     1081 acggcat
//
LOCUS       DAQSWQ010000284         1085 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.286, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000284 DAQSWQ010000000
VERSION     DAQSWQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.286"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..643)
                     /locus_tag="R8G18_004454"
     CDS             complement(<1..643)
                     /locus_tag="R8G18_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEE9797964.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="R8G18_004455"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="R8G18_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HEE9797965.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAQSWQ010000285         1052 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.287, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000285 DAQSWQ010000000
VERSION     DAQSWQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.287"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..155
                     /locus_tag="R8G18_004456"
     CDS             <1..155
                     /locus_tag="R8G18_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HEE9797966.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            259..>1052
                     /locus_tag="R8G18_004457"
     CDS             259..>1052
                     /locus_tag="R8G18_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEE9797967.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          301 a          218 c          245 g          288 t
ORIGIN      
        1 gtactgccga aaatattcag atagagctga gggatgacca ggatgctgcg ttaaaaaatg
       61 gcgatagcaa aacggttatt gttgatgaga tcactcgtaa tgcacagttt ccacttaagg
      121 caagagctat cacggtgaat ggaaacgcaa gctagggaac gatcgaggcg ctaatcaatg
      181 tgatctacac ctggcaataa cgtcgattcg attttcttag ccttcttttt aaatcaattt
      241 ttaaccttag gatatatcat gggtaaaaca atcagtataa aggtcctgtt cggtatttat
      301 cttctattaa tggcaggcaa agtttttgcc ttcagttgta atgtcgatgg cgggtcaagt
      361 attggagcag gtacaacctc tgtttatgtt aatctcgacc ctgtaataca gccgggccag
      421 aatctggttg tagacttgtc tcagcatata agttgctgga atgattacgg cggctggtac
      481 gacactgatc atataaacct ggtacaaggc tcagctttcg caggctccct acagtcatat
      541 aaaggatcgc tgtactggaa taatgtgacc taccccttcc cgttgacgac aaataccaac
      601 gtattggata ttggtgataa gacaccgatg ccattaccgt taaaactcta tattacccca
      661 gttggggcgg ccggtggagt tgtcataaaa gcgggggaag tcattgcccg tattcatatg
      721 tataaaattg caacattagg aagcggaaat ccgcgtaatt ttacgtggaa tattatttca
      781 aataacagtg ttgttatgcc aacaggcggc tgcaccgttg atagtcgtaa tgtcactgtg
      841 gatctcccgg actttcctgg aagcgcagaa ataccccttg gcgtatattg ctcaagcgaa
      901 caaaagctct cattttatct ttcgggtgcc accactgaca gtagccgaca agtgtttgct
      961 aacacagcac ctgatgcaac aaaagcatcg ggagtgggtg tcactctaat gcgtaatgga
     1021 aaaatactgg ctacgggaga aaatgtctcc ct
//
LOCUS       DAQSWQ010000286         1049 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.288, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000286 DAQSWQ010000000
VERSION     DAQSWQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.288"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..120
                     /locus_tag="R8G18_004458"
     CDS             <1..120
                     /locus_tag="R8G18_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HEE9797968.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="R8G18_004459"
     CDS             120..410
                     /locus_tag="R8G18_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HEE9797969.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="R8G18_004460"
     CDS             407..961
                     /locus_tag="R8G18_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HEE9797970.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          299 a          201 c          298 g          251 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca tttgaaagag cccgcttta
//
LOCUS       DAQSWQ010000287         1023 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.289, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000287 DAQSWQ010000000
VERSION     DAQSWQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.289"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(77..865)
                     /locus_tag="R8G18_004461"
     CDS             complement(77..865)
                     /locus_tag="R8G18_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEE9797971.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="R8G18_004462"
     CDS             complement(905..>1023)
                     /locus_tag="R8G18_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEE9797972.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAQSWQ010000288         1016 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.290, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000288 DAQSWQ010000000
VERSION     DAQSWQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.290"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..494)
                     /locus_tag="R8G18_004463"
     CDS             complement(<1..494)
                     /locus_tag="R8G18_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797973.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDA"
     gene            complement(786..>1016)
                     /gene="tuf"
                     /locus_tag="R8G18_004464"
     CDS             complement(786..>1016)
                     /gene="tuf"
                     /locus_tag="R8G18_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEE9797974.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
BASE COUNT          315 a          229 c          205 g          267 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
      481 atatttaatt tcattataat cccttgtgac gtaaaaactg caaaacaaaa gtcctacgca
      541 ggaattattt acgttttacg agaacccttc aatatgaatt aaagtgaggc attaaaaata
      601 agaaaaaagc ctgacaaatg aagaatttta aaaacagaaa cattcatatt taaaatatta
      661 aattgaattg atattttaaa tatgaataat ttattcgttc tgacagtacg aataagatat
      721 gcagtcaaca aatgcaaaaa gggcgccgaa gcgccctttt caattcaaaa ctaattaacg
      781 tgtaattagc ccagaacttt agcaacaacg cccgcgccaa cggtacggcc gccttcacgg
      841 attgcgaaac gcagaccgtc gtccatcgcg atcgggtgga tcagggtaac aaccattttg
      901 atgttgtcgc ccggcattac catctctacg ccttccggca gttcgatggt accagtcacg
      961 tcagtagtac ggaagtagaa ctgcggacgg tagcctttga agaacggagt atgacg
//
LOCUS       DAQSWQ010000289         1009 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.291, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000289 DAQSWQ010000000
VERSION     DAQSWQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.291"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..164
                     /locus_tag="R8G18_004465"
     CDS             <1..164
                     /locus_tag="R8G18_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HEE9797975.1"
                     /translation="SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAH
                     HQVDIDLED"
     gene            170..>1009
                     /locus_tag="R8G18_004466"
     CDS             170..>1009
                     /locus_tag="R8G18_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEE9797976.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIA"
BASE COUNT          306 a          229 c          246 g          228 t
ORIGIN      
        1 aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca atcagccacg
       61 catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggctg
      121 aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta tggcgctttt
      181 tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt ttgctggcga
      241 cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta tgcggcaacg
      301 gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag gctctgactg
      361 ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa cccgctcgtt
      421 tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa tgatcatcga
      481 ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc gaaaccgtaa
      541 acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac gctttaccga
      601 tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat atgagtatga
      661 ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa aaatgaaagc
      721 agaaacagaa accggttatg aaccctccat cctgatccag atggagaagc aaatgaacat
      781 agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca cccgtattga
      841 tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg agtttcttaa
      901 cctgggaggt gaacacctcg gcatcgatac ctcacgggat aacagtgaat tatttgccaa
      961 cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgca
//
LOCUS       DAQSWQ010000290          985 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.292, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000290 DAQSWQ010000000
VERSION     DAQSWQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.292"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            230..>985
                     /gene="gspA"
                     /locus_tag="R8G18_004467"
     CDS             230..>985
                     /gene="gspA"
                     /locus_tag="R8G18_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HEE9797977.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          280 a          199 c          237 g          269 t
ORIGIN      
        1 gcatctttat tatgattcgc cagatggaag gggaaacgta gtgtgggcac gatgtatgtt
       61 ctaataaaat agattgggtg ggttgtatat tgacgttagt ataaatagta ttaatgccgc
      121 ttgcatgaat caagcaaaat ttatttatat tagtaatatg tatttaatta ataaatacat
      181 acattatttt tatgattaag gataatcaat aattaaaaca ggaagttcta tgtctacgag
      241 aagagaagtt attctttcct ggttgcgtga gaaacgtcag acctggcgtc tgtgctattt
      301 gttgggtgag gctggaagtg gaaaaacctg gctggcgcag caactgcaaa aagataaaca
      361 tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa ggtaaggccg catggatcgt
      421 taccgacgat aacgcggctg aacagggctg ccgtgacagc gcctggacgc gagatgagat
      481 ggcggggcaa ttactgcatg cgcttcatcg aactgatagt cgctgccctc ttattattat
      541 tgaaaatgct cacctgaatc accgccgcat acttgatgat ctccaacgcg cgatatctct
      601 tattcctgac ggtcaatttt tgctgatcgg cagacccgat cgaaaagtcg aacgtgattt
      661 taaaaaacag ggcattgaac ttgtctcgac aggacgtctg acggagcacg aacttaaggc
      721 aagcatcctt gaaggtcaga atattgacca acccgatctc cttttaacct ccagagttct
      781 gaaacggata gctttattat gtcggggcga tcgcagaaag ctggcgcttg ccggcgaaac
      841 aattcgctta ttacagcaag ccgagcagac cagcgttttc actgcaaaac aatggcgaat
      901 gatttaccgc atattgggtg ataaccgccc ccggaaaatg cagcttgccg tggtaatgag
      961 tggaaccatt atcgctatta cctgt
//
LOCUS       DAQSWQ010000291          971 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.293, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000291 DAQSWQ010000000
VERSION     DAQSWQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.293"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..133)
                     /locus_tag="R8G18_004468"
     CDS             complement(<1..133)
                     /locus_tag="R8G18_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEE9797978.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="R8G18_004469"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="R8G18_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          297 a          220 c          184 g          270 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggtgact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtgt tggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccacgcctta tatccctgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taagcctgga gattatctac accagcatgg
      661 acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa agtattagag
      721 ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaatagaac
      781 taatatcaag cggcgctata ttgcgtatta aataaaacca cctcaattac attaataaat
      841 ctaaacaaac atcatgcaaa taatttccat ggttatttct aggctattta aattaaataa
      901 ataaattatg agtcaatcat aaataacaac ctttaatata atttaactac caaatttata
      961 ttcgttcaca a
//
LOCUS       DAQSWQ010000292          970 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.294, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000292 DAQSWQ010000000
VERSION     DAQSWQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.294"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            548..952
                     /gene="stpA"
                     /locus_tag="R8G18_004470"
     CDS             548..952
                     /gene="stpA"
                     /locus_tag="R8G18_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HEE9797979.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          276 a          215 c          230 g          249 t
ORIGIN      
        1 ctggacggat tcgtaatcgc tggattcggt cattctgtct cccttgtata tgggttaacc
       61 agataaccac ggggagcagg taccagcatc cacgttacag acggtcccgg caaaagtgcc
      121 tgacctgacc cctattagcg gttaccagcg ccccaccaga cccggtgaca tcaccccccg
      181 gatcatggac gactgggggc agtaatcatg ccgggtctgg ctggctaaca ccccattata
      241 aggggtacga ataaagtaac gggggttctt ttattctgtt atattgcatt tttattcact
      301 tcggttttgt caggcttgcg gaattagcga gcagagagcg ccgttctgtt caccaataat
      361 ctaaattact catcaaaatg gctaatatat aatcttgaaa ttattctgag agtttcagaa
      421 aataacagct gaaataatct cgcgcaagac tgtaaataga ttaaattttg tggaaatata
      481 ataagtgatc gcttacacta cgcgacgaaa tacttttttg ttttggcgtt aaaaggtttt
      541 ctttattatg tccgtaatgt tacaaagttt aaataacatt cgcaccctcc gtgcgatggc
      601 tcgcgaattc tccattgacg ttcttgaaga aatgctcgaa aaattcaggg ttgtcactaa
      661 agaaagacgt gaagaagaag aacagcagca gcgtgaactg gctgagcgtc aggaaaaaat
      721 tagcacctgg ctggagctga tgaaggctga cggaattaac ccggaagagt tattgggtaa
      781 tagctctgct gctgcaccac gcgctggtaa aaaacgccag ccgcgtccgg cgaaatataa
      841 attcaccgat gttaacggtg aaactaaaac ctggaccggt cagggccgta caccgaagcc
      901 aattgctcag gcgctggcag aaggtaaatc tctcgacgat ttcctgatct aattactgag
      961 gccggatgtc
//
LOCUS       DAQSWQ010000293          960 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.295, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000293 DAQSWQ010000000
VERSION     DAQSWQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.295"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>960
                     /gene="htrE"
                     /locus_tag="R8G18_004471"
     CDS             <1..>960
                     /gene="htrE"
                     /locus_tag="R8G18_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HEE9797980.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQ"
BASE COUNT          278 a          194 c          238 g          250 t
ORIGIN      
        1 tgggcttccg gacaaaatcg tagcaattac tctattggct acagtaacag tgcatcctgg
       61 ggcagctaca gtgtcagtgc ccagcgttca tggaatgaag acggcgacac tgacgatagc
      121 gtttatctca gtttcaccat tcctattgaa aaattacttg gcactgaaca acggacttca
      181 ggtttccaga gtattgatac tcaaatgagc agtgacttta agggtaataa ccaactcaac
      241 gtcagtagca gtggctatag tgataacgct cgcgtcagtt atagcgtaaa tactggctat
      301 acgatgaata aagccagcaa agatttgagt tatgttgggg gttatgccag ctatgaatca
      361 ccgtggggaa ccctggcagg ttcagtttct gcaaatagcg ataacagtcg tcaagtgtct
      421 ctcagcaccg acggtggttt tgtattgcat agcggcggcc tgaccttcag taatgacagt
      481 tttagcgact ccgatacact ggcggtagtt caggctccag gtgctcaagg agcgcgaata
      541 aattatggca acagtactat cgatcgatgg ggttatggtg tcaccagcgc tctttctcct
      601 tatcatgaaa accgtatcgc gctggatatc aacgatcttg agaacgatgt tgaattaaaa
      661 agtaccagtg cagtagctgt accgcgtcag ggttcagtcg tctttgctga ttttgaaacc
      721 gtgcaagggc aatcagccat tatgaacatc acacgaagtg atggtaaaaa tattccattt
      781 gctgcagata tttatgatga gcaaggcaat gtcattggta atgttggaca gggtggacaa
      841 gcatttgttc gtggcattga gcagcaggga aatatcagca ttaaatggct cgaagaaagt
      901 aaacccgtaa gttgtcttgc gcattatcaa caaagttcag aagcagaaaa aatagcgcaa
//
LOCUS       DAQSWQ010000294          935 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.296, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000294 DAQSWQ010000000
VERSION     DAQSWQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.296"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(197..>935)
                     /locus_tag="R8G18_004472"
     CDS             complement(197..>935)
                     /locus_tag="R8G18_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEE9797981.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          259 t
ORIGIN      
        1 ttgcttctcc gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact
       61 tgttgaccga gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga
      121 ttatcctgtc tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt
      181 tactgctggc atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac
      241 ctaatataac cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata
      301 tcacttccgt caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac
      361 ccattccttt tgtcatcaga acagatgcca gagggataac cgcctcagcc cggatataca
      421 gcggaatacc gacaacggcg cttaatggga tggcgaaggg attatctgct cctgcatgag
      481 cggcaatcca cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca
      541 aaagatatgg cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg
      601 gattaatact tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt
      661 tagccgctgg actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt
      721 ttgctggagc acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc
      781 ccagagaatc caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca
      841 acaaggtgac tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta
      901 atggtgaaac aaataaaaaa gtgagagtcg gacca
//
LOCUS       DAQSWQ010000295          905 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.297, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000295 DAQSWQ010000000
VERSION     DAQSWQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.297"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..796
                     /locus_tag="R8G18_004473"
     CDS             <1..796
                     /locus_tag="R8G18_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEE9797982.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITL
                     EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGY
                     ENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
BASE COUNT          259 a          182 c          197 g          267 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
      361 tctttactac gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga
      421 tgttctgata aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt
      481 agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg aatttaccaa
      541 agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg
      601 gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa
      661 tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggt tgttcgccca gcgattaccg
      721 taggcaattt aaaaattgtt ttgcggaaca agaaatctta tctgaatttc ctcaaccggt
      781 aagtcttgcc ggataaatct ggcggcgcac tttgttaaaa agctgcgccg cctgcagatt
      841 atttgttctg atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg
      901 taccg
//
LOCUS       DAQSWQ010000296          903 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.298, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000296 DAQSWQ010000000
VERSION     DAQSWQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.298"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="R8G18_004474"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="R8G18_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HEE9797983.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          266 a          144 c          221 g          272 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctg
//
LOCUS       DAQSWQ010000297          888 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.299, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000297 DAQSWQ010000000
VERSION     DAQSWQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.299"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..568)
                     /locus_tag="R8G18_004475"
     CDS             complement(<1..568)
                     /locus_tag="R8G18_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEE9797984.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGL"
     gene            complement(568..>888)
                     /locus_tag="R8G18_004476"
     CDS             complement(568..>888)
                     /locus_tag="R8G18_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEE9797985.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          297 a          210 c          193 g          188 t
ORIGIN      
        1 caagacccat taagatccct ggcaaatatc ccgccataaa tagcgatgct acagaaacgc
       61 cgccagctgt cagggaaaac acaattaaca cgtttgaggg cggtatcagt aatccagtaa
      121 tacaagatga gacgtttaca gcggtagaaa atgccggatc ataaccttgt ttggtctgaa
      181 tgggattcag tgtgccacca acggcagcag ctgccgcaac ggctgaacca gaaatggaac
      241 caaacatcat attcgccagg acgttcacat gaccaagtga accaggaacc cgaccaacca
      301 ttacctgggc gaggttaatc aaacgtatcg caataccgcc gctgttcatc agcgttccgg
      361 caaagatgaa aaaggggatt gccaataggg caaagttatc cagtccatta gccagtcgct
      421 gcgaaacagt gattgccgcg atatcccagg gaaacatcaa aagcattgaa gctacagtgg
      481 caataccgat tgagaacgaa atgggcacac caataaagac cagcacaaaa aaacagccaa
      541 acagtgtaat cgcgatataa ctatccatta actggcctcc tgcgcaggtt gtttcagttg
      601 ttgaatgctt tgattaataa aacacaaggc ataaaacatc atcaccaatc cacttaatgg
      661 cagaatgata tatacgtatc ccatcggtat ttgcatcgcg gcagatacct gtgatgttgc
      721 cagcgtttta tcaattaatt tcaggccacc agtcacaatg accgaaccag caaaacctaa
      781 aataagaacg ttaatgacta tactaagaag taattgcttg cgcttattca gggcgagagc
      841 aaataaatcg atagagagat gtctctgcgc gccgacggta tatgcagc
//
LOCUS       DAQSWQ010000298          881 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.300, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000298 DAQSWQ010000000
VERSION     DAQSWQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.300"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            73..462
                     /locus_tag="R8G18_004477"
     CDS             73..462
                     /locus_tag="R8G18_004477"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEE9797986.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISSEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            459..806
                     /gene="tnpB"
                     /locus_tag="R8G18_004478"
     CDS             459..806
                     /gene="tnpB"
                     /locus_tag="R8G18_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEE9797987.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          220 a          239 c          228 g          194 t
ORIGIN      
        1 gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta
       61 gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc
      121 cctaattatc ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata
      181 tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga
      241 caacaatggc gcgagggaaa gctgctatta ccttcttcag agagccccca gctacttcct
      301 gtgactctcg atgcagctgc cgaacagcca gaatcgctcg cagaggaccc gaaaaccctc
      361 agtatcagca gtgaggtaac gttccggcac gggacgctcc gcttcaatgg caatgtcagc
      421 gaaaagctcc tgactctgct gatacaggaa ctgaagcgat gatcccgtta ccttccggga
      481 tcaaaatttg gctggttgcc ggtatcaccg atatgagaaa tggcttcaac ggcctggctg
      541 cgaaagtaca gacggcgctg aaagacgatc ccatgtccgg ccatgttttc attttccggg
      601 gccgcagcgg cagtcaggtt aaactgctgt ggtccaccag tgacggactg tgcctcctga
      661 ccaaacggct ggagcgtggg cgcttcgcct ggccgtcagc ccgtgatggc aaagtgttcc
      721 ttacacaggc gcagctggcg atgctgctgg aaggtatcga ctggcgacag cctaagcggc
      781 tgctgacctc cctgaccatg ctgtaaatct ctttatcctg gttgtcacag aataagcccg
      841 gtaaaatacg ggcttatgaa cgacatctct tctgacgaca t
//
LOCUS       DAQSWQ010000299          877 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.301, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000299 DAQSWQ010000000
VERSION     DAQSWQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.301"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>877
                     /gene="cadA"
                     /locus_tag="R8G18_004479"
     CDS             <1..>877
                     /gene="cadA"
                     /locus_tag="R8G18_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HEE9797988.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          221 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgcac
//
LOCUS       DAQSWQ010000300          872 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.302, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000300 DAQSWQ010000000
VERSION     DAQSWQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.302"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(292..>872)
                     /locus_tag="R8G18_004480"
     CDS             complement(292..>872)
                     /locus_tag="R8G18_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEE9797989.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          257 a          177 c          160 g          278 t
ORIGIN      
        1 atatccgtac ctatccggat atctaatgtc ataagcatcc ctttaattat cccctcaacc
       61 tcttgtaact ttcccactct tgaactccac atagtccaaa cgccggatgc aaaaaaatca
      121 cacgtgcatt tctgaaattg tgatggagga agatagtatc aattatatgt gcttataaac
      181 gtccccgatt agcgcagaag tcaccggatt gttcaggctc caataggttg agtatgccgt
      241 atttattttc caaaataaaa atcgatgctg ttgctccttc ataaaatact tttactctct
      301 ggacgctttt cttagctctc ttggtgttat cttaaaacga ttatggaacc tttcagtaaa
      361 acgagaagga cacttgtaac cattttccct ggcaatctcg ctgatgggtt ttactgtcgt
      421 ttgtatagca gacaacgcat tatttaacct cacgtcgtcc aatatacttt gaaaacttac
      481 tccctcgctt gccagacggc gatgcaatgt ggaaacagaa atgtagagat atctcgctac
      541 cttgtttgct gtccattttg ttccaggttc ggatagcatt aggttataac aacgacttat
      601 taatgattgt ttactatctg ataaaagtaa acaattaaca tgatgtgctc ctaatgaaag
      661 tagaacgccc attgctaagt gttcctgaat ttgcgttgag aagcctcggg aaatagatgt
      721 ttttagttgc tcccaacaat atattaactc ggaattctga ggtaagcaga aacctgtcgt
      781 gttacgaatt tgatcggtta tcgcataaag attttggaac ttttcaatta gatcaatggg
      841 ataataaagc atttctgcaa gataaagacg gt
//
LOCUS       DAQSWQ010000301          820 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.303, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000301 DAQSWQ010000000
VERSION     DAQSWQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.303"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..372)
                     /locus_tag="R8G18_004481"
     CDS             complement(<1..372)
                     /locus_tag="R8G18_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797990.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGP"
     gene            complement(323..>820)
                     /locus_tag="R8G18_004482"
     CDS             complement(323..>820)
                     /locus_tag="R8G18_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEE9797991.1"
                     /translation="VLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRYCYWPISR
                     LTGWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVRGQSY
                     RGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCALSGVPDAHVSGAMPGL
                     FIRTS"
BASE COUNT          176 a          225 c          207 g          212 t
ORIGIN      
        1 gggaccagca ttccagatac gaccgttcca ttcaaacgtg aacggcttcg cctcctgttc
       61 tgcgcgccat gcttcaattt cctgacgcct ggcctctctg gccgcttcca gcatttctgg
      121 tgtcacagtg aatggggcta tctcacccca tttgccactt tgcagttcct gccagatttg
      181 ctgacccgtc ggtgcgacat catcagcggt ggctgtgtag gggactgcct ggtccctgtc
      241 gtcaaaaaaa acgtcacagt ctactgcgcc actttcggta taacggggat taatgatttt
      301 tttaatttcc acggtgcatt cctcacgatg tgcgaataaa aagcccgggc attgcgccag
      361 agacatgtgc atccggcacc ccggacaggg cgcaatatga ccccggtaat gaatgctctg
      421 aacatcccgt aatgaaaaat tgtggggatg ctatatacgt tccggtggga gtactgggca
      481 ctgaaatccc caccggtccc agtcgtgagc ctctgtatga ctgccccctg acaagtctga
      541 tgactttatc accgtcagct tctccctggt acgcagcaat aatcagcccg ccaatgtcag
      601 ggtctcccca tctgttgcgg acagagctcg ccacgattct gtaaataata tcttctgtgg
      661 ttatatttat tttcacccag ccagtcagtc tggatatggg ccagtagcag tagcgggtgt
      721 gataaatggg gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg
      781 ttgtctcagg gcgtgcatca gtccaccgga tgctgagcac
//
LOCUS       DAQSWQ010000302          815 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.304, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000302 DAQSWQ010000000
VERSION     DAQSWQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.304"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(246..>815)
                     /locus_tag="R8G18_004483"
     CDS             complement(246..>815)
                     /locus_tag="R8G18_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEE9797992.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAQSWQ010000303          808 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.305, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000303 DAQSWQ010000000
VERSION     DAQSWQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.305"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            248..544
                     /locus_tag="R8G18_004484"
     CDS             248..544
                     /locus_tag="R8G18_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797993.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDLNPRRKKTYQEALIGCCSVLICGVDWRRMLHHANHKR"
     gene            complement(433..789)
                     /locus_tag="R8G18_004485"
                     /pseudo
     CDS             complement(433..789)
                     /locus_tag="R8G18_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
BASE COUNT          242 a          190 c          191 g          185 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cttaatccgc ggcgaaaaaa gacataccag gaggcgctaa tcggttgttg
      481 tagtgtgctc atttgcggtg ttgactggcg gcgcatgctg caccacgcaa accataagag
      541 ataagcgccg ccgacgattc tgataagcga aaaaatctcc tcacactgcg ttattagcgt
      601 tgcaagacca aacaaaccca acccggaata aaatgcatcg cccagcgcca cgcccagtcc
      661 ggtcagcacc cctgcgcgtc gaccggaagc caggctggtt tgtactacca caaagagatt
      721 ggctcccgga ttaaaaaaag taatcacgaa cagtcctacg gtcaggtaaa cggcgtgcaa
      781 aggatccata gtgatttcat ccataaat
//
LOCUS       DAQSWQ010000304          803 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.306, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000304 DAQSWQ010000000
VERSION     DAQSWQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.306"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..741)
                     /locus_tag="R8G18_004486"
     CDS             complement(<1..741)
                     /locus_tag="R8G18_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEE9797994.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          269 a          137 c          162 g          235 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttagat tga
//
LOCUS       DAQSWQ010000305          790 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.307, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000305 DAQSWQ010000000
VERSION     DAQSWQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.307"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>790
                     /locus_tag="R8G18_004487"
     CDS             <1..>790
                     /locus_tag="R8G18_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HEE9797995.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          281 a          115 c          134 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc
//
LOCUS       DAQSWQ010000306          790 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.308, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000306 DAQSWQ010000000
VERSION     DAQSWQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.308"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            52..790
                     /locus_tag="R8G18_004488"
                     /pseudo
     CDS             52..790
                     /locus_tag="R8G18_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAQSWQ010000307          789 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.309, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000307 DAQSWQ010000000
VERSION     DAQSWQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.309"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..458)
                     /locus_tag="R8G18_004489"
     CDS             complement(<1..458)
                     /locus_tag="R8G18_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HEE9797996.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="R8G18_004490"
     CDS             complement(530..742)
                     /locus_tag="R8G18_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9797997.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          173 g          220 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAQSWQ010000308          786 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.310, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000308 DAQSWQ010000000
VERSION     DAQSWQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.310"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            211..>786
                     /locus_tag="R8G18_004491"
     CDS             211..>786
                     /locus_tag="R8G18_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEE9797998.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRC"
BASE COUNT          199 a          201 c          186 g          200 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatacttc cgatcattga ttggaattcg ccagtcactc
      121 cgggaattaa ctcttttcac cacagtctcc attgccgtgt ttaaaccagt ggagacgtca
      181 atgtccaaaa agagaaaatc caggagcact atggaaactt gttttaagat ccttcagctc
      241 aagttcgata agaagctgac taaccgttgt atcggactga cgttacacat cagcgcctca
      301 accgtttttg aggttcttgc ccgcttcaaa gcctcttctc tgtcatggcc attacctgcg
      361 gatatatccc atgacacgct ggagaaactc attttcccgc ccaaagacac ctctgcgtca
      421 gagcttgtta tgcctgacat gctgtacttc gatacggaaa tgagaaaacc tggcgtgacg
      481 cgccaactgt tgtggatgga atacaaggca caggcgggtg acaaagcgat gggatactca
      541 cacttctgcc gttgctaccg gaagtggaaa aaaacgcgac gcctctccat gcgacaggag
      601 catcgtgctg gcgaaaagct ctttatcgac ttctgtggtc ctacggtccc tgttattaat
      661 ccggacaccg gagagatacg ccgcgtggca atcttcgtgg ctgtaatggg ggcgtccaac
      721 tatacctatg ttgaagcgtg cgaaggtcag gacatgatgt catggttaaa tgcccacagc
      781 cgttgc
//
LOCUS       DAQSWQ010000309          775 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.311, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000309 DAQSWQ010000000
VERSION     DAQSWQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.311"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..343
                     /locus_tag="R8G18_004492"
     CDS             <1..343
                     /locus_tag="R8G18_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEE9797999.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>775)
                     /locus_tag="R8G18_004493"
     CDS             complement(405..>775)
                     /locus_tag="R8G18_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HEE9798000.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaa
//
LOCUS       DAQSWQ010000310          749 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.312, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000310 DAQSWQ010000000
VERSION     DAQSWQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.312"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            582..>749
                     /locus_tag="R8G18_004494"
     CDS             582..>749
                     /locus_tag="R8G18_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HEE9798001.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          134 c          164 g          238 t
ORIGIN      
        1 tgcttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc atatttgaac
       61 gttctgccgg aatgcattat caatagaggt aaagtcgcaa ccccaaatcg taaaggaaac
      121 cgtagcacgt cgtatgcaag aacgtgccac ggctggctga tggatgttcg atagcgcgag
      181 tttgaatgaa aatcagccgg agatgatttt acataattgc tacggaatta ttcaatacag
      241 gaattgcttg cgtctgcatg gattgacctg aaatattccc gaaaatttct ctaaaaaact
      301 cgaaaaaaat ggtaactggt tgaatgtatt aatatgcaat ggtacgtgtc agggattaaa
      361 agatgaacgt aaatttattt aacgcattaa ttttaaaggg ttttattgtt tgttgacgaa
      421 aacaggaatc gtgttcggtc tctttttatc tgttaaaagc cagaagcatt tccttcgctg
      481 actttatagt caaccataac acacactcta ctgtctgagt ccaacgtttt ttaacattct
      541 tgttaaaatt atgtgatctt tagcgcggga ggaaaatatt gatgaaacag cctgcgcccg
      601 tttatcagag aattgcgggt catcaatggc gacatatctg gctttctggc gatatacacg
      661 gttgtcttga gcagttgcgc cgcaaattat ggcattgtcg ttttgatccg tggcgagatt
      721 tacttatctc agtgggagac gttatcgat
//
LOCUS       DAQSWQ010000311          748 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.313, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000311 DAQSWQ010000000
VERSION     DAQSWQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.313"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..407
                     /locus_tag="R8G18_004495"
     CDS             <1..407
                     /locus_tag="R8G18_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEE9798002.1"
                     /translation="WRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYT
                     QRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWY
                     NNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            449..649
                     /locus_tag="R8G18_004496"
                     /pseudo
     CDS             449..649
                     /locus_tag="R8G18_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          216 a          175 c          198 g          159 t
ORIGIN      
        1 ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tatgggcccg tcgaccgtcc ggcacggtcc atcacagtga taaaggttct cagtatgtat
      121 cgctggccta cacacagcgg cttaaggaag ccggattact ggcatcaaca ggaagtacag
      181 gcgactcgta tgacaacgcg atggcggaga gcatcaatgg tctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccatactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tactcctccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcaa ccttcacgat aaaaaacttt tcattaaagg
      481 taaagcggat caatatccgg cacttcattc tctgatatct ccgacccgca ttaatctgaa
      541 agaaatagaa attcactggc gtgaagtgct acgtctggcg acctcaataa aacagggaac
      601 agtgacagca tccctgatgc tgagaaaact tgccagttac cccaaacaaa agtaaggcag
      661 tatgactttc cgtgagacag atgctggcca acgtgggata tttaacgcgg gttaacatcg
      721 ttgtcagagc cgcatgggtg agctgttg
//
LOCUS       DAQSWQ010000312          745 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.314, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000312 DAQSWQ010000000
VERSION     DAQSWQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.314"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..397)
                     /locus_tag="R8G18_004497"
     CDS             complement(<1..397)
                     /locus_tag="R8G18_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEE9798003.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          174 c          108 g          230 t
ORIGIN      
        1 ccaacctgga ccatgctaaa ttcacaccgt gctgcgcggc ctgattaaat agtgcatacg
       61 ctttggcata atccacccgc acctgctcac cgtcgtagta gatacccccc agcgctaacg
      121 gagcttctct gtcaccttgt ttagccgcta actcgaacca tttaatcgct tcctgaacat
      181 caggtttggt atcataacga ggctgataat attcccagcc taacaggctc tgcgcgtcga
      241 tatctcccga ttcagccatt ttatgcagtg tatttataac atcgggagca ggtttaccac
      301 cgtatatgct gtcatacaat tcatgcgctt tctcgctggc caccacgctt ggtgaaacta
      361 tcagcattac agaaaataat attcttttag ttaacatgta tacctttatg tcctcactgt
      421 catatatttc cataataatt aaccaccaaa tatccattta atgaataaat acagtgccat
      481 aaaggtccaa gtaataagca cacatttagc ctgaaacatt atcatccatg tcaccaaatc
      541 gtttatttag tgagataaaa ctagcatatt cgtaagataa aaaatgcaca tggcaatcaa
      601 gaaatgatat tattactttt ttgatgtaaa actatttagt tttaaaattc attttttcaa
      661 catatagagc attcatttta tgaatgcaaa aaaacatacc tgacacacaa attactctcc
      721 tctgtaatat tttctcatta acact
//
LOCUS       DAQSWQ010000313          736 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.315, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000313 DAQSWQ010000000
VERSION     DAQSWQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.315"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(1..736)
                     /locus_tag="R8G18_004498"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="R8G18_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAQSWQ010000314          726 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.316, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000314 DAQSWQ010000000
VERSION     DAQSWQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.316"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            35..726
                     /locus_tag="R8G18_004499"
                     /pseudo
     CDS             35..726
                     /locus_tag="R8G18_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAQSWQ010000315          703 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.317, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000315 DAQSWQ010000000
VERSION     DAQSWQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.317"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..703
                     /locus_tag="R8G18_004500"
                     /pseudo
     CDS             1..703
                     /locus_tag="R8G18_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          142 a          233 c          187 g          141 t
ORIGIN      
        1 ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc ccgctcagaa
       61 ggagtccttg tcggatacct ccctggactg gccatgcgcc gcatagccga cttacacgcc
      121 ggtgaagcta aaactgatgc tcgtgacgct gccatcattg ccgaagctgc ccgtaccctg
      181 cctcacgcgc tacgcacgct gaaactggct gacgagcaaa tcgccgaact ctccatgctc
      241 tgcggcttcg atgatgatct tgccgcacag acaacgcagg ccagcaaccg tatccgcggc
      301 cttctgaccc agatacatcc ggcactggag cgcgttctcg gtccgagact tgagcacccg
      361 gcggtactcg atcttctcca gcgatatccc tcaccagaaa aactcgcttc gctgggcttc
      421 gctgggtgag aagaagctgg cagcccagct ctgcaaactt gcgcctcgtc tgggtaaacg
      481 ccttgcagca gacatagctc aggcactggc cgaacaaacc gtcgtcgttc ccggcacgaa
      541 tgccgctgcc gtagtactgc cacgtctggc actccagctc atcacgctgc gtaagcaaag
      601 agacgaggtg gcgcttgagg tagaacagcg agttcttgct caccctcttt acccggtcct
      661 gaccagtatg cccggagtcg gtgtcaggac cgcagccaga ctc
//
LOCUS       DAQSWQ010000316          701 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.318, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000316 DAQSWQ010000000
VERSION     DAQSWQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.318"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            132..>701
                     /locus_tag="R8G18_004501"
     CDS             132..>701
                     /locus_tag="R8G18_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HEE9798004.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELD"
BASE COUNT          196 a          159 c          196 g          150 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga c
//
LOCUS       DAQSWQ010000317          698 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.319, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000317 DAQSWQ010000000
VERSION     DAQSWQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.319"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>698
                     /locus_tag="R8G18_004502"
     CDS             <1..>698
                     /locus_tag="R8G18_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9798005.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          182 g          140 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaa
//
LOCUS       DAQSWQ010000318          695 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.320, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000318 DAQSWQ010000000
VERSION     DAQSWQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.320"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>695
                     /gene="tuf"
                     /locus_tag="R8G18_004503"
     CDS             <1..>695
                     /gene="tuf"
                     /locus_tag="R8G18_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEE9798006.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAQSWQ010000319          690 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.321, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000319 DAQSWQ010000000
VERSION     DAQSWQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.321"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
BASE COUNT          241 a          104 c          144 g          201 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg aggtaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tttgaacaga ctgtttaagt aaagcctata
      421 aagtatctat taattaaggg ctatgtctca atactagcag tttgtactag cagtttgtca
      481 atacctttgt ctttagcaat tcgtgctatt tattaaatat agctgttaaa acagagtatg
      541 ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg ttcttagtga
      601 gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga agaacgagga
      661 tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAQSWQ010000320          683 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.322, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000320 DAQSWQ010000000
VERSION     DAQSWQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.322"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..560
                     /locus_tag="R8G18_004504"
     CDS             <1..560
                     /locus_tag="R8G18_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HEE9798007.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
BASE COUNT          244 a          121 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acg
//
LOCUS       DAQSWQ010000321          678 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.323, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000321 DAQSWQ010000000
VERSION     DAQSWQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.323"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..361
                     /locus_tag="R8G18_004505"
                     /pseudo
     CDS             1..361
                     /locus_tag="R8G18_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          194 a          170 c          171 g          143 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaatt
//
LOCUS       DAQSWQ010000322          667 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.324, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000322 DAQSWQ010000000
VERSION     DAQSWQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.324"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>667
                     /locus_tag="R8G18_004506"
     CDS             <1..>667
                     /locus_tag="R8G18_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HEE9798008.1"
                     /translation="ITFIADDNNAELIPCLSTDLLVSLGIKKIALLDNKEHSAEKHVP
                     DNSACTPLQDRLADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAGLLN
                     YSFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKW
                     QHINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVI
                     HGIA"
BASE COUNT          203 a          129 c          145 g          190 t
ORIGIN      
        1 atattacttt tattgcagat gataataacg cagaactcat cccatgcctg agtacagatc
       61 ttcttgtcag tcttggtata aaaaaaatag cgttattaga caataaagaa cattcggcag
      121 aaaaacatgt gccggacaac agcgcatgca caccacttca ggatcgtttg gccgacgcat
      181 caactgagtt tgatgttggt cagcaacatc tgtcactctc cgttccgcaa atttatgttg
      241 gcagaatggc tcgcggctat gtttctcctg atctgtggga agaaggaata aatgcagggc
      301 ttctaaacta tagttttaat ggtaattcta ttaataatcg tagtaaccat aatgcaggaa
      361 aatcaaacta tgcatatttg aatttacaga gtggcatcaa cattggtagt tggcgactac
      421 gcgataactc aacgtggagt tataacagtg ggagtagcaa ttcatctgac agcaataaat
      481 ggcagcatat caatacgtcg gctgaacgtg acattattcc cttacgctca cggttaacgg
      541 taggtgatag ttataccgat ggcgatattt ttgatagtgt gaactttcgt ggcctcaaaa
      601 taaattcaac agaagcgatg ttgcccgata gacaacatgg tttcgctccg gtgattcatg
      661 gtattgc
//
LOCUS       DAQSWQ010000323          650 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.325, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000323 DAQSWQ010000000
VERSION     DAQSWQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.325"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            65..337
                     /locus_tag="R8G18_004507"
     CDS             65..337
                     /locus_tag="R8G18_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9798009.1"
                     /translation="MDIGAILALVISGCVFLYTIFRDNTKDTNDLLTRVATLETTIAV
                     LESSISRIEDDQDKMRDTLSKLETQIHELDVKIERIIAILQQKQRP"
     gene            complement(376..>650)
                     /locus_tag="R8G18_004508"
     CDS             complement(376..>650)
                     /locus_tag="R8G18_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEE9798010.1"
                     /translation="SEADKLLSRDLAKTIMQVQTLGLNLPDDWNDFIIIMTFQLGIGG
                     VKKFRKMLAALKAHDYLEAIKQAKCSLWYRQTPNRVNSMIAVLKNK"
BASE COUNT          212 a          119 c          113 g          206 t
ORIGIN      
        1 aatccaataa caataacaat aacaataaca ataacaataa caataacaac aaagggattt
       61 cagtatggat atcggtgcta ttttagctct tgttatttct ggatgcgtat tcctttatac
      121 tatctttaga gataatacaa aggatacgaa tgatctatta actcgcgttg ctactcttga
      181 aactaccatt gctgtactgg aaagtagtat ttcccgtatt gaggatgatc aggataagat
      241 gagagataca ctaagtaaac tggaaacaca aatccatgaa cttgatgtta agattgaaag
      301 gattattgca attcttcagc agaaacaacg accataaaaa aaggcatagt atgtcattcg
      361 tactatgcct tttctttatt tgtttttaag tactgcaatc atggaattca cacggtttgg
      421 tgtctgcctg taccatagag aacacttagc ctgtttaatt gcttcaaggt aatcatgtgc
      481 tttcagggct gctaacatct tacggaattt cttcaccccg ccaataccta actggaaggt
      541 catgataata ataaaatcat tccagtcatc gggaagattt aaccctaatg tctgtacctg
      601 cataattgtt ttggctaaat cgcgagatag taacttgtct gcttcgcttt
//
LOCUS       DAQSWQ010000324          650 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.326, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000324 DAQSWQ010000000
VERSION     DAQSWQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.326"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..194
                     /locus_tag="R8G18_004509"
     CDS             <1..194
                     /locus_tag="R8G18_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HEE9798011.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
     gene            complement(195..386)
                     /locus_tag="R8G18_004510"
     CDS             complement(195..386)
                     /locus_tag="R8G18_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HEE9798012.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(491..>650)
                     /locus_tag="R8G18_004511"
     CDS             complement(491..>650)
                     /locus_tag="R8G18_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HEE9798013.1"
                     /translation="LTIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQS
                     DAWIMRLA"
BASE COUNT          184 a          148 c          160 g          158 t
ORIGIN      
        1 acgttgattt gcatcaggtc ttgtggagcc gctcgcgatt aggtgaacgc caaaaagggc
       61 agggaattac aggtgctgat catttctggt ttggtcatac accgttgcga catcgcgtgg
      121 atattggcaa cctgcattat attgataccg gtgctgtctt tgggggcgaa ctgactcttg
      181 tgcaattgca ataattaaaa atcaccgtac tcctgtgcag gtcgccagaa accatctata
      241 aaatcctcaa tcggaaaaca accgccatgg cggatccgtt gatcgctcat agaataaaga
      301 cactgctgtt ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag
      361 caaacaaaaa gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt
      421 ttaggagaga ttttacaacg ggggaataac caggacaaat aacccgccat cccgacgggt
      481 tctttttgga tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac
      541 ggtaaaggac agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg
      601 catcgagttg catacataaa tcggggtcag atttacaagg aatggttaac
//
LOCUS       DAQSWQ010000325          649 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.327, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000325 DAQSWQ010000000
VERSION     DAQSWQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.327"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(7..330)
                     /locus_tag="R8G18_004512"
                     /pseudo
     CDS             complement(7..330)
                     /locus_tag="R8G18_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="R8G18_004513"
                     /pseudo
     CDS             384..587
                     /locus_tag="R8G18_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          158 a          175 c          153 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaac
//
LOCUS       DAQSWQ010000326          647 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.328, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000326 DAQSWQ010000000
VERSION     DAQSWQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.328"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>647
                     /gene="istA"
                     /locus_tag="R8G18_004514"
     CDS             <1..>647
                     /gene="istA"
                     /locus_tag="R8G18_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HEE9798014.1"
                     /translation="NETFHTLRALNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAP
                     ALSPLPLEPYEYTEYKAVKVGPDYHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHK
                     GQCVAQHPRSTHEYKHTTNPLHMPERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLK
                     AKPHPELAYRAVLGLLALQKKYGPERLEKACYVALHYNAPDRRFIDNLLRHHRDN"
BASE COUNT          163 a          191 c          173 g          120 t
ORIGIN      
        1 taatgaaacc ttccacacgc tcagggcgct gaatgcacgc cttcgggagt tgctgacgga
       61 catgaataac cgaccaatga agggatacgg taatcaaacc cgcgcagaac gcttccgcat
      121 gctggatgca ccagcactct ctccgctgcc tctggagcct tatgaataca cggaatacaa
      181 agccgtgaag gttggccctg actaccacgt tgaatatgcc cgtcactggt actccgttcc
      241 acacgaactg gtcggtcagc gtctgtcact gaaggtgggg cagtcggtgg tacagctctg
      301 gcacaagggg cagtgtgtgg cgcaacatcc gcgcagcaca catgaatata aacacacaac
      361 caatccgctt cacatgccag aacgccaccg gcggcatggg acatggacac cggaacgcct
      421 tatcgagcag ggaaaccgta caggtccgtc cacaggccgg gtggttgaat ccatgctgaa
      481 agccaaaccc catccggagc tggcttaccg tgcagtcctg ggtctgctgg cacttcagaa
      541 aaaatacggc ccggaacggc tggaaaaggc ctgttacgtt gccctgcact ataacgcccc
      601 ggacagacgc tttatcgaca atctgctgcg tcatcatcgg gataacg
//
LOCUS       DAQSWQ010000327          642 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.329, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000327 DAQSWQ010000000
VERSION     DAQSWQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.329"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(1..609)
                     /locus_tag="R8G18_004515"
                     /pseudo
     CDS             complement(1..609)
                     /locus_tag="R8G18_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
BASE COUNT          190 a          160 c          146 g          146 t
ORIGIN      
        1 gttactacca atccgattct gtacactatt tccgctgaaa ttataattga gcaagccggc
       61 attaataccg ggatcccaca actcaggagg aatatatcca cgcgcgcgat tactcataaa
      121 tgcctgaggg atcgtcaggt tgagtcgctg ctgaccaaca tctaaatgcg cagtagcgtc
      181 atggatcatt gaggttaatg gcacgcaggc atcatccacc agcagattca taccggagac
      241 agaagccgta ttcagcccca tactggcaag ctgcgcgcgt gtcaggcagg gaacaatccc
      301 ttgttcactg tcgcccgtgt taaatgtgac atcacgcgtt gccatataac cattattcaa
      361 atagatatcg acgcgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa
      421 atcggccaca gcctagggat catccgctaa aaagcgcgga ttaaaataga gttcggcaga
      481 tgacaaaggt gcctgtgcgg caaaagcaca ggcgacaaag agccggacaa aaagaccagc
      541 caaacgatgc ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag
      601 atatgacatt attcctccgg tttaaaatca cccggcagtc aa
//
LOCUS       DAQSWQ010000328          641 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.330, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000328 DAQSWQ010000000
VERSION     DAQSWQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.330"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="R8G18_004516"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="R8G18_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEE9798015.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAQSWQ010000329          636 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.331, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000329 DAQSWQ010000000
VERSION     DAQSWQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.331"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(16..204)
                     /locus_tag="R8G18_004517"
     CDS             complement(16..204)
                     /locus_tag="R8G18_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEE9798016.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(215..427)
                     /gene="cspG"
                     /locus_tag="R8G18_004518"
     CDS             complement(215..427)
                     /gene="cspG"
                     /locus_tag="R8G18_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HEE9798017.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
BASE COUNT          181 a          133 c          143 g          179 t
ORIGIN      
        1 cgagggagcc tgatattatt ttgtgaaatc cagcggcgca tggtcagtgc gtatttgcag
       61 cgcgacgtta tcaaaggtga tcatcgtcga tttacaaaaa atacacttag cgcccaaagg
      121 gtttcgctcc gtcacatcga atgccgatgt gcggtactgt gaaccatgac aacatggaca
      181 gcggaaatgg agatgttgag taataacggc aaccttagag cgtaacaacg ttcgctgccg
      241 cggggccacg ttgcccctgc tcaatagaaa attcaacttt ctgattttcg ttcagcgtgc
      301 ggaattcatt gctctggatg gcggtgaaat ggacgaaaac gtctttgctg ccatcatcag
      361 gagtgataaa gccaaaacct ttatctgcgt taaaccattt tactaaacca gtcattttat
      421 tagacatatt ttacttcctt cattttgggc ctttcggcaa acagggcttt aatacagaaa
      481 ctacttagtg ttcaggagag agactcaaaa gaaggggata atgcctgata atgagaactg
      541 ctttagtaaa ctactttgta tgctgtctgt ctttcaaacc gacgcagcta ttaacgcatg
      601 ataattgaga ttaagcaaat tttattttag ccgtcc
//
LOCUS       DAQSWQ010000330          627 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.332, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000330 DAQSWQ010000000
VERSION     DAQSWQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.332"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..546
                     /locus_tag="R8G18_004519"
     CDS             <1..546
                     /locus_tag="R8G18_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9798018.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
BASE COUNT          175 a          126 c          191 g          135 t
ORIGIN      
        1 cagatccaga acattggcgt taatcagata cagacggttg gtgtcaacca ggtggaaacg
       61 gtgggcagta accagattat caaagtggga tcaaaccagg ttgaaaaggt ggggatcatt
      121 cgtgcgctga cggtgggtgt ggcttaccag acgacggtag gcggcattat gaatacctcg
      181 gtggcgttgt tgcagtcctc acaggtaggg ctgcataaat cactgatggt gggaatgggc
      241 tacagcgtca atgtagggaa taacgtcacc ttctcggtgg gcaagacgat gaaggaaaac
      301 accggacaaa cagcagttta ttctgccggt gagcatcttg aactctgctg tggtaaggca
      361 aggctggtgc tgacgaagga cggaagcata tttctcaacg gtacgcacat tcatctggaa
      421 ggggagtcgg atgtgaacgg tgatgcgcca gtgattaact ggaactgtgg tgccacacaa
      481 cctgtaccgg atgcgcctgt gccgaaagat ttaccccccg gaatgccgga tatgcggcaa
      541 ttttgagtca ggtgcaaaaa attaaaacgg aactggtgca gtgaccagat gaaccatatt
      601 aaaagcagaa ggaattgatt catgggc
//
LOCUS       DAQSWQ010000331          593 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.334, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000331 DAQSWQ010000000
VERSION     DAQSWQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.334"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="R8G18_004520"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="R8G18_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HEE9798019.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAQSWQ010000332          591 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.335, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000332 DAQSWQ010000000
VERSION     DAQSWQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.335"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..365
                     /locus_tag="R8G18_004521"
                     /pseudo
     CDS             1..365
                     /locus_tag="R8G18_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            275..502
                     /locus_tag="R8G18_004522"
     CDS             275..502
                     /locus_tag="R8G18_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HEE9798020.1"
                     /translation="MLPENAGSDQHNDSNNETGEDELNWKRQVLIAAVYGLCANPACI
                     AYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            480..>591
                     /locus_tag="R8G18_004523"
     CDS             480..>591
                     /locus_tag="R8G18_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HEE9798021.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEK"
BASE COUNT          201 a          120 c          158 g          112 t
ORIGIN      
        1 gattagcgac ccgaatgaac cgaaatggat tcccggtcat catatctcct caaatgaagt
       61 caaaaaaacg gaaaatgaag tgcgacagac tgaagataaa cagcaccaga acagcgaacc
      121 ggaggaggga acaccctgca atcagactgg cgaggataac tgccctgact gtggcgcggt
      181 gatgggagac gcaacttatc aggaaacttt tgacgaaaaa aaaccagatg aagcacagga
      241 agaagagccg aagaaaacgg aaaaagctga tgatttgtta ccggagaacg ctggcagcga
      301 tcagcataat gatagcaata atgaaactgg cgaagatgaa ctcaactgga aaagacaggt
      361 gttgattgcg gcggtttacg gtttatgtgc gaatcctgcg tgcatagcct atgcgcctgc
      421 aatccctgac atcgcaatga tgattgccaa caagcttgaa aattttggag tgaccagtga
      481 tgagcgctta cccgattttt gatcgtattg aagaaatggc atgggctcgt cactaccagc
      541 agatcgttcg tgaagaaaaa gaaacggaac tggcggacga cctggaaaaa g
//
LOCUS       DAQSWQ010000333          565 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.336, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000333 DAQSWQ010000000
VERSION     DAQSWQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.336"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..216
                     /locus_tag="R8G18_004524"
     CDS             <1..216
                     /locus_tag="R8G18_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEE9798022.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="R8G18_004525"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="R8G18_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..>565)
                     /locus_tag="R8G18_004526"
     CDS             complement(415..>565)
                     /locus_tag="R8G18_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEE9798023.1"
                     /translation="DQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPGLKRALKRLQKG
                     DALVV"
BASE COUNT          159 a          137 c          115 g          154 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgaccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtca
//
LOCUS       DAQSWQ010000334          561 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.337, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000334 DAQSWQ010000000
VERSION     DAQSWQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.337"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..526)
                     /locus_tag="R8G18_004527"
     CDS             complement(<1..526)
                     /locus_tag="R8G18_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HEE9798024.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKLSEEE"
BASE COUNT          146 a          167 c          128 g          120 t
ORIGIN      
        1 attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc aaacacaagc
       61 ggagcaaatg ctcgtcttgc catagatctt cacgaatatt aggatcgaag ctgacaaaac
      121 ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag
      181 acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg
      241 tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt tccccttgat
      301 cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc agatacgtga
      361 tatcgactcc ctcagttagc agcgttcttt gcattaacgc cccaaaagga tcatcaccga
      421 cccgacctat aaatccactt attccgccta atctggcgat tcccaccgca acgttagctg
      481 gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga tctacgaccg
      541 catcccctaa aacccatact t
//
LOCUS       DAQSWQ010000335          559 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.338, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000335 DAQSWQ010000000
VERSION     DAQSWQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.338"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..428
                     /locus_tag="R8G18_004528"
     CDS             <1..428
                     /locus_tag="R8G18_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HEE9798025.1"
                     /translation="QLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGRE
                     FAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKK
                     RRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          163 a          124 c          156 g          116 t
ORIGIN      
        1 aacagcttac cagcgtggcg aggcagaatg aaacctgtaa gcggttgctg gatattcctg
       61 gcgttggacc acttattgcg acggcagcgg tggccaccat gggggaagca tcagcgttta
      121 aatcggggcg agagttcgcc gcatatgttg gtctggttcc aaaacaaact ggctccggag
      181 ggaaagtacg tctgctgggg ataagcaaac gtggtgacac ttatctcagg acattattta
      241 tccacggtgc aagagcggtg gcattagtag ctaaagagcc tggcccgtgg ataaccgaac
      301 tgaaaaaacg tcgtccagcc agtgtggcaa tcgtcgccat ggcaaacaag ctggcacgaa
      361 cagtatgggc gataaccgcc catgaccgta agtatgacag gaaccacgtc agtatcagac
      421 catattaatc gctgatacca ttaaacaatg aactcttaac aaaagggtga atgctgaaag
      481 gttgctatgg cggccagagt gatgacaaag acaggtaaga ccgtgactca ctaaacctga
      541 acagtatttt gggcttgaa
//
LOCUS       DAQSWQ010000336          555 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.339, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000336 DAQSWQ010000000
VERSION     DAQSWQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.339"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            78..555
                     /locus_tag="R8G18_004529"
                     /pseudo
     CDS             78..555
                     /locus_tag="R8G18_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          136 a          121 c          123 g          175 t
ORIGIN      
        1 agctgataga aacagaagcc actggagcgc ctcaaaaaca ccatcataca ctaaatcagt
       61 aagttggcag catcaccgcc gttgtctaca catttattct ctcggtgctg atttaccggg
      121 aagtaaaatg gcgccattta cccaaactga ttctggagtc ggttgttacc acttcgattg
      181 tattactgtt gatcggtttt tccgtgggca tgtcctgggc aatgactaat gctgatattc
      241 cttatatgat cagcgacgca ttgatgggga tttctgataa cccactgatc atattgctgc
      301 tgattaatat cgtcttatta attgtcggca tctttatgga catgacaccg gctgtactaa
      361 tctttacacc gatcttcttg ccgattgcac aagagctggg aatggaccca gtacatttcg
      421 gtattatgat ggtggccaac ctttgtattg ggctgttaac accgccggtg ggtagtgcgc
      481 tatttgttgg ttgttctatc tcgggtgtga agattcaaca tttaatcaaa ccattgctgc
      541 cattttatgc tagca
//
LOCUS       DAQSWQ010000337          553 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.340, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000337 DAQSWQ010000000
VERSION     DAQSWQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.340"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>553
                     /gene="feaR"
                     /locus_tag="R8G18_004530"
     CDS             <1..>553
                     /gene="feaR"
                     /locus_tag="R8G18_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HEE9798026.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQ"
BASE COUNT          140 a          125 c          146 g          142 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aat
//
LOCUS       DAQSWQ010000338          550 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.341, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000338 DAQSWQ010000000
VERSION     DAQSWQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.341"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..550
                     /locus_tag="R8G18_004531"
                     /pseudo
     CDS             1..550
                     /locus_tag="R8G18_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          195 a          120 c           83 g          152 t
ORIGIN      
        1 ttatccgact tattaaaaat gcctgtttaa aattatataa tttagcacat accgcaacca
       61 actcccacct taaaccaact ggtccggata attcagatgt tctcctgttc aaaaagctat
      121 tctccccttc gaaattaatg acaattatcg gtgatgaaat tcctcttata tctgaaaaac
      181 agtcgctttc aaaggtgctt ttaaatgatg agaataatga actgagtgat ggtacaaact
      241 tctgggataa aaatcgtcaa ttaaccacag atgaaatagc ttgctatctt cagaagatcg
      301 ccgctaatgc aaaaaatact caagtcaatt atcctactgg tctctacgtc ccctactcca
      361 ccagaactca cctggaagac gctctcaatg aaaatattaa gagcgatcca tcatggccga
      421 atgaagtcca gttattcccc ataaatactg gcggacactg gatattagtt tcgctacaga
      481 aaatagtaaa taaaaaaata ataaactaca aataaaatgc gtcatattca actcattgcg
      541 tgcactaggc
//
LOCUS       DAQSWQ010000339          546 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.342, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000339 DAQSWQ010000000
VERSION     DAQSWQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.342"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..546
                     /locus_tag="R8G18_004532"
                     /pseudo
     CDS             1..546
                     /locus_tag="R8G18_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAQSWQ010000340          544 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.343, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000340 DAQSWQ010000000
VERSION     DAQSWQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.343"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            227..484
                     /locus_tag="R8G18_004533"
     CDS             227..484
                     /locus_tag="R8G18_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HEE9798027.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          195 a           78 c           95 g          176 t
ORIGIN      
        1 caactttgat ggcatagata atgtttttag taaaaaagag ttgactgaat ataatacagc
       61 ctcttcctac ataagaagga atgtgctcac aaaaaaaaac cattagtctg cttcctgttc
      121 ttacaataat cagtattatg tattttattt atatagcaaa atatgcttgt tacacctgtc
      181 ataattaggc atggtaaaat tattgactga accctggggg aggaagatgc acagtgatta
      241 ttataatatg gtttttggtg aaaaattggc caatatttta tatgaggcta actctcaatt
      301 tttttacgag agaaatgtca tagaagaggc agtcaacgct ttattctgtg agcgtgaaat
      361 aataaacaac aagaacataa taaagaagct gatgttcttt ttgtcagatg ttaatcacac
      421 caagaaagat gttgttcaga gcgcacttaa tattatcatt gacattacat caggtgatat
      481 ttgagatttt acataaaaga taatctctca aggagtctta tctgaaaacg aaatatatag
      541 tccc
//
LOCUS       DAQSWQ010000341          519 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.344, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000341 DAQSWQ010000000
VERSION     DAQSWQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.344"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..>519)
                     /locus_tag="R8G18_004534"
     CDS             complement(<1..>519)
                     /locus_tag="R8G18_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEE9798028.1"
                     /translation="LGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIGV
                     LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGSL
                     LMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNTV
                     QDLMNTIINMTA"
BASE COUNT          142 a          140 c          132 g          105 t
ORIGIN      
        1 cggcggtcat attaataata gtattcatca gatcctgcac ggtattggaa ccgagcatcg
       61 ggataatcat cagcgggatg acgatcagga actggatcca cgctgcgcgc gccggaacgc
      121 cgttttcgtt cagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
      181 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacatcgcg gtaaaggaca
      241 ccagaccgac aaagcgattc atcaacgctt ccggtagacc aaaatacgcc gccatgccgt
      301 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
      361 tcagcaccga ggagacggaa tacagtacgc cgataaaaat tccggcgagg atgatcactt
      421 taacgaacga tttcgaaccg cctttgacgt cgttaacgta cacagcgacg gactccgcgc
      481 cacctgcggc cataaagatc caggtggtaa cgccgagga
//
LOCUS       DAQSWQ010000342          518 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.345, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000342 DAQSWQ010000000
VERSION     DAQSWQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.345"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..413
                     /gene="ygeH"
                     /locus_tag="R8G18_004535"
     CDS             <1..413
                     /gene="ygeH"
                     /locus_tag="R8G18_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HEE9798029.1"
                     /translation="LTEDGILTQGNEQVYIPQKELGVLIVLLESAGHVVLKDMIIESV
                     WKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKINEDNTSDY
                     SIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT          173 a           86 c           96 g          163 t
ORIGIN      
        1 cgctcacgga agatggaatt cttactcaag gaaatgagca agtttatatt ccacagaaag
       61 agttaggtgt attaatagta ttacttgaat ccgctggtca tgttgtactg aaagatatga
      121 tcatcgaatc agtatggaaa aatattattg ttagtgacga gtccctgaca agatgtatct
      181 attctttgcg ctgcattttt gaaaaaattg gctatgatcg ttgcatagaa acaatctacc
      241 ggaaaggtta tcgtttcagc gggcaggttt tcaaaactaa aattaatgaa gataatactt
      301 cagactattc catagctata ttccctttca ctacttcatt gaatacactg gatccattaa
      361 tacttaatca ggaattagtg caaatcattt caaataaaaa aatcgatggt tagactggcc
      421 ccctgaatct ccagacaacc agtatcactt aaataagtga tagtcttaat actagttttt
      481 agactagtca ttggagagca gatgattgat gtcttagg
//
LOCUS       DAQSWQ010000343          508 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.346, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000343 DAQSWQ010000000
VERSION     DAQSWQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.346"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            12..119
                     /locus_tag="R8G18_004536"
     CDS             12..119
                     /locus_tag="R8G18_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HEE9798030.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          128 a          121 c          154 g          105 t
ORIGIN      
        1 caggcggcta tatgacgctc gcagaactgg gcatggcctt ctggcatgat ttagcggctc
       61 cggtcattgc tggtattctt gccagtatga tcgtgaactg gctgaacaag cggaagtaac
      121 gtgccatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggatcag gccgcagggg
      181 tgaaactctg cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga taatattctg
      241 cggaatgcgc agcacagagt gaagacgtga cggattccgg aacagaaaaa cagtcgtaga
      301 atcagcgtca agccggaagg gttccggtga ggcgcaatgt tgcgggggct ttatccctgg
      361 tggcattggt tgctggagag agaaaacccc cgcacgttgc aggtatgcac ctgacaacat
      421 cacgggggcc attcttgaac ctgaacaact caagaatagc cgcgaaacgt tgtcattaca
      481 acacaggcgg ctatatgacg ctcgcaga
//
LOCUS       DAQSWQ010000344          495 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.347, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000344 DAQSWQ010000000
VERSION     DAQSWQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.347"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>495
                     /locus_tag="R8G18_004537"
     rRNA            <1..>495
                     /locus_tag="R8G18_004537"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          107 a          111 c          164 g          113 t
ORIGIN      
        1 cgaccttgaa ataccaccct ttaatgtttg atgttctaac gtggacccgt gatccgggtt
       61 gcggacagtg tctggtgggt agtttgactg gggcggtctc ctcctaaaga gtaacggagg
      121 agcacgaagg ttggctaatc ctggtcggac atcaggaggt tagtgcaatg gcataagcca
      181 gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc aggtcatagt gatccggtgg
      241 ttctgaatgg aagggccatc gctcaacgga taaaaggtac tccggggata acaggctgat
      301 accgcccaag agttcatatc gacggcggtg tttggcacct cgatgtcggc tcatcacatc
      361 ctggggctga agtaggtccc aagggtatgg ctgttcgcca tttaaagtgg tacgcgagct
      421 gggtttagaa cgtcgtgaga cagttcggtc cctatctgcc gtgggcgctg gagaactgag
      481 gggggctgct cctag
//
LOCUS       DAQSWQ010000345          491 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.348, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000345 DAQSWQ010000000
VERSION     DAQSWQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.348"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            285..389
                     /locus_tag="R8G18_004538"
                     /pseudo
     CDS             285..389
                     /locus_tag="R8G18_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          159 a          100 c           99 g          133 t
ORIGIN      
        1 tatcatacag ataattactg gttaacccta tatagggcat tttaaagtgt tgtgatctta
       61 cccagcaata gtggacacgc ggctaagtga gtaaactctc agtcagaggt gactcacaag
      121 acaaaacagt ttcaaccagt aaaaaaacgt aaacaccatt cgcatgaatt tcgtagttaa
      181 gatctgaagc ttgcagaatg catcggtgtt actgccggag tccgtgaact tagtctgcat
      241 gaatcaccac agctttacat ctgtcgcagt aaacagcaaa atcagcaaac ttacttaatt
      301 atatcgagtg cgattacaat cgctggcgat ggtacagttg gtgtggcggt ctcagtccgg
      361 aacaatttga aaatcagaat ctcacttaaa gcggcatcca ttttacaaaa gatggccgtc
      421 tgtgtatttg taatgaatac cttctacatg tttagagtgc ataatccacg acaaggcgat
      481 atcctaaaaa t
//
LOCUS       DAQSWQ010000346          489 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.349, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000346 DAQSWQ010000000
VERSION     DAQSWQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.349"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="R8G18_004539"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="R8G18_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HEE9798031.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAQSWQ010000347          476 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.350, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000347 DAQSWQ010000000
VERSION     DAQSWQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.350"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>476
                     /locus_tag="R8G18_004540"
     rRNA            <1..>476
                     /locus_tag="R8G18_004540"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAQSWQ010000348          469 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.351, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000348 DAQSWQ010000000
VERSION     DAQSWQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.351"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>469
                     /locus_tag="R8G18_004541"
     rRNA            <1..>469
                     /locus_tag="R8G18_004541"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          129 a           99 c          145 g           96 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaacc
//
LOCUS       DAQSWQ010000349          468 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.352, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000349 DAQSWQ010000000
VERSION     DAQSWQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.352"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            85..447
                     /locus_tag="R8G18_004542"
     CDS             85..447
                     /locus_tag="R8G18_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEE9798032.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          114 a          109 c          137 g          108 t
ORIGIN      
        1 gtggtgatgt ctatctcaca ccgatgaaca tgaccacgaa accctccgat ggcagtaagg
       61 ccggtaagca gaaggataac gccaatgcag acgaaacaac gtcttgatgt accgctgagt
      121 ctgaaatctg tcagtgactc cggtgagttt gaagggtatg gctccgtctt tggtgtaaag
      181 gacagccacg atgatgtggt gatgtccggg gcatttgctg cttccctgcg ggcgtggagt
      241 gacagaaaag cgttacctgc gctgctctgg cagcaccgca tggatgaacc catcggtgtt
      301 tacaccgaaa tgaaggaaga cgatgtcggg ctttacgtca ggggacggtt gcttattgat
      361 gatgatcccc tcgcaaaacg tgcacatgca cacatgaagg ccggttcgtt aaccggcctt
      421 tctattgggt acgtcctgaa agactgaggt agcctgagtt taacggac
//
LOCUS       DAQSWQ010000350          467 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.353, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000350 DAQSWQ010000000
VERSION     DAQSWQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.353"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>467
                     /locus_tag="R8G18_004543"
     CDS             <1..>467
                     /locus_tag="R8G18_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEE9798033.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALG"
BASE COUNT          119 a          108 c          152 g           88 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cactggg
//
LOCUS       DAQSWQ010000351          459 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.354, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000351 DAQSWQ010000000
VERSION     DAQSWQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.354"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..389
                     /locus_tag="R8G18_004544"
     CDS             <1..389
                     /locus_tag="R8G18_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HEE9798034.1"
                     /translation="IKGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDV
                     YNSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNII
                     CSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          136 a           96 c          122 g          105 t
ORIGIN      
        1 aaattaaagg gcaggttgtt gaacttgtac gtctgagcgg tcgcgagacg ttgcggcaac
       61 tggaagccaa gacaggtgcg acaagatatc tgatgagtgt tctcgccaga gagctggttg
      121 ccagtggcga tgtatacaac tctggttacg ggttattccc gtctgaacag gcgcgtaagg
      181 actggcaaaa cgcccgcaaa aaactctcaa gggcaaatct gaagaaacca tctgtggttg
      241 atcctgacct tatctggtca ttaccagacg gagaaatacg ccgctacgac aggcgtcaaa
      301 acataatctg tagcgagtgc cggaagagcg aagctatgca gcgtgtactg gctttctatc
      361 agggtaattt tcagaaggtg ctgttgtgag ccaaattaac aatcggaact tcgtgaagag
      421 aaagcataat ccaaaatctg aataattaag ttcagcact
//
LOCUS       DAQSWQ010000352          457 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.355, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000352 DAQSWQ010000000
VERSION     DAQSWQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.355"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..118
                     /locus_tag="R8G18_004545"
     CDS             <1..118
                     /locus_tag="R8G18_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HEE9798035.1"
                     /translation="KLTKSECEKMLSGEKEAGASREQKVKLINFNCEKLQSS"
     gene            225..>457
                     /locus_tag="R8G18_004546"
     CDS             225..>457
                     /locus_tag="R8G18_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEE9798036.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT          161 a           93 c           99 g          104 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgcttc
       61 cagagagcag aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat
      121 ttcattatat ccagattaga acttcatagc tgattattaa aaatcaacca cacccgccag
      181 tattctgtat atttactggc ggtcatatcg tgagaggtat ggcaatgaat cttgtgacac
      241 tcaaaacgtg gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac
      301 gatgggcaag gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg
      361 tggttccgga ggctttctat atcaacccaa ataaggttga taccgatata acacaccatc
      421 agcctaatgg gcgacaaggg agagacagtc cgttaat
//
LOCUS       DAQSWQ010000353          442 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.356, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000353 DAQSWQ010000000
VERSION     DAQSWQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.356"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>442
                     /locus_tag="R8G18_004547"
     CDS             <1..>442
                     /locus_tag="R8G18_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HEE9798037.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAQSWQ010000354          435 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.357, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000354 DAQSWQ010000000
VERSION     DAQSWQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.357"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>435
                     /locus_tag="R8G18_004548"
     CDS             <1..>435
                     /locus_tag="R8G18_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEE9798038.1"
                     /translation="HIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKALA
                     EKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGVC
                     GGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          108 a           84 c          123 g          120 t
ORIGIN      
        1 ggcatattgc tgccaatatt cagtttccac caaattttga tgagtcaaaa cagtatccgg
       61 cgattatcag cgtccatcct tttggcagtt gtaaggagca gacgtctggc aatatctatg
      121 gcaaggcatt ggctgaaaaa ggctatctcg ttctcgctta tgacgccagt ttccagggag
      181 aatctggtgg ggagcctcgc tggatagaag atccgacaca gcgcgtagag gatattagcc
      241 gtgttatcga ttatgcagta actctgccat atgttaatgc tgaacgtatt ggcgtgctgg
      301 gagtgtgtgg gggtggtggc tatgccatta atgccgcgct gacagaaaaa cgaattaaag
      361 cagtagtgag tattacgggt gttaatatcg ggcgtctttt tcgggaaggt ttcagcaatt
      421 acgatccgat tggcg
//
LOCUS       DAQSWQ010000355          421 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.358, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000355 DAQSWQ010000000
VERSION     DAQSWQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.358"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            42..117
                     /locus_tag="R8G18_004549"
     tRNA            42..117
                     /locus_tag="R8G18_004549"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           99 g          126 t
ORIGIN      
        1 gtttattgcg tggtttataa gtattgttat tgcacactca aggcccttta gctcagtggt
       61 tagagcaggc gactcataat cgcttggtcg ctggttcaag tccagcaagg gccaccaaat
      121 cattatttac gtatcaggaa gagttaaggg tacaaggggt actcgatggc ggtctatatt
      181 tcatgactaa cgccatttca aggaagaagt gcggtgggtg gtttcgaatg agggctggtg
      241 ttttggtacg tggcacgcca ctgcaatgta tttatccatc agtactcatt caaaagtacc
      301 tggaaaaaat gcctgctatg ccaagcgggc aaattgctta catgtggaat taagtaatta
      361 acgataaagc cagagtatta tcatttatta taccccttca tttaatcagg ttaattactc
      421 a
//
LOCUS       DAQSWQ010000356          413 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.359, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000356 DAQSWQ010000000
VERSION     DAQSWQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.359"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..187
                     /locus_tag="R8G18_004550"
     CDS             <1..187
                     /locus_tag="R8G18_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEE9798039.1"
                     /translation="RPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIW
                     DMGRIAMSVAQQPQCHK"
BASE COUNT          125 a           96 c          103 g           89 t
ORIGIN      
        1 ccgccccgtc attgacagag catgggatgc tcaactcagg ctttgtaaga ggtatcgaaa
       61 acttcaggcc aaaggaaaga atgtcaatat tacaattgtt gctgttgcac gtgagctggc
      121 gggttttatc tgggatatgg gccgaatagc aatgtctgtc gcacaacaac cacaatgtca
      181 caaataaaaa atgagaataa ccagttccct gaaggcgacg tagcccggta caagagtaac
      241 ccacgttagc gcttggcgac ggcggaacaa agctgattcg cgacgtaaga aagtggcagg
      301 ctcatatgac ggacctctgt aatgcggtat ccaacccgcg aatatcagcg tgattcaccg
      361 tcggatttac tgctacgtcg ccctcaggga attaaaccta aaaagaaaat cgt
//
LOCUS       DAQSWQ010000357          412 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.360, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000357 DAQSWQ010000000
VERSION     DAQSWQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.360"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            41..253
                     /locus_tag="R8G18_004551"
     CDS             41..253
                     /locus_tag="R8G18_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9798040.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            286..>412
                     /locus_tag="R8G18_004552"
     CDS             286..>412
                     /locus_tag="R8G18_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HEE9798041.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGK"
BASE COUNT          145 a           68 c           90 g          109 t
ORIGIN      
        1 cactgtaaat aaaatttaat ccttaactgg aggtatatct atgtcaaata cacagaaaat
       61 tattaacact gaaaaatata acgagtgggt gaaaaaattc tctgagcaga tttttaaaat
      121 tactggcgac gagaatgtgg caaaaaatga attagaaccg tggacacctg aaggaaacgc
      181 accaaattat tgctggtggg aggttgatcc ggttgatgct gcaaatgaag ccatgagtta
      241 ccacaacgat taatgtcggg aggccgcccg aaagggcggt aagaaatgac tacattattc
      301 agaaaagaat atccgcaaaa aagtagggcg acagaatttt tgtttctcat tctgtttatc
      361 gtattgatga taccgatatc ccctctaatt tttgtctggg caatcgggaa aa
//
LOCUS       DAQSWQ010000358          408 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.361, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000358 DAQSWQ010000000
VERSION     DAQSWQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.361"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            54..130
                     /locus_tag="R8G18_004553"
     tRNA            54..130
                     /locus_tag="R8G18_004553"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="R8G18_004554"
     tRNA            173..248
                     /locus_tag="R8G18_004554"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAQSWQ010000359          399 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.362, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000359 DAQSWQ010000000
VERSION     DAQSWQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.362"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>399
                     /locus_tag="R8G18_004555"
     CDS             <1..>399
                     /locus_tag="R8G18_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HEE9798042.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatgac
//
LOCUS       DAQSWQ010000360          390 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.363, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000360 DAQSWQ010000000
VERSION     DAQSWQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.363"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..>390)
                     /locus_tag="R8G18_004556"
     CDS             complement(<1..>390)
                     /locus_tag="R8G18_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEE9798043.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIR"
BASE COUNT          120 a           78 c           82 g          110 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt
//
LOCUS       DAQSWQ010000361          389 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.364, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000361 DAQSWQ010000000
VERSION     DAQSWQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.364"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(<1..>389)
                     /locus_tag="R8G18_004557"
     CDS             complement(<1..>389)
                     /locus_tag="R8G18_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HEE9798044.1"
                     /translation="KMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLW
                     LVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEV
                     LPAPLPPYRVLTGLVDRFGRTQTFHRE"
BASE COUNT           71 a          134 c          112 g           72 t
ORIGIN      
        1 ttcgcggtgg aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca gtacccggta
       61 cggcggcagc ggcgcaggca gcacctcatc cgcttccggc acccgctcac accagccgag
      121 cagccaccac ggcccctgcg gactgtttgt cgccagataa cgatgcggac ttaagcggag
      181 ttcttccggc agcgcctgcc agagtgcggc cagccggtga ccttcatcca gtttcgccac
      241 gccgccgcgc accagccaga gtgattcgct gcggctgtaa ccgtcctcac cgggaaacag
      301 gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt atcagtgtgt tatcgcgcag
      361 ctgtaagcgg atatccgcag gcattttcc
//
LOCUS       DAQSWQ010000362          379 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.365, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000362 DAQSWQ010000000
VERSION     DAQSWQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.365"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
BASE COUNT           92 a          104 c           79 g          104 t
ORIGIN      
        1 tctgtgacgt ccccatggac tgtctaaaac ttcgacgtag tctctgtgac cgcagactgt
       61 ctaaaacctg gacgtagtct ctgtgcctgc agactgtcta aaacctgttc gtagtctacg
      121 tggcctcccc acagaccgtc taaatcctgg acgtagtctt tgtgaccgca gactgtttaa
      181 aacctggacg tagtctctgt gacatccccc aaattgttta aaacctggac gtagtctccg
      241 tgacatcccc acagactgtc taaaacctgg aagtagtctc ttcttagaca ttttagacag
      301 gctgtctaaa attttgacat ggtctctgtg actgcagact gtctaaaacc tggacgtagt
      361 cgccatgacg tccccacaa
//
LOCUS       DAQSWQ010000363          367 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.366, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000363 DAQSWQ010000000
VERSION     DAQSWQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.366"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>367
                     /locus_tag="R8G18_004558"
     CDS             <1..>367
                     /locus_tag="R8G18_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEE9798045.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSN"
BASE COUNT          106 a           85 c          107 g           69 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaact
//
LOCUS       DAQSWQ010000364          360 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.367, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000364 DAQSWQ010000000
VERSION     DAQSWQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.367"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            complement(16..285)
                     /locus_tag="R8G18_004559"
                     /pseudo
     CDS             complement(16..285)
                     /locus_tag="R8G18_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          103 a           90 c           62 g          105 t
ORIGIN      
        1 gcataatccc aatgattact ccctgacagg atttgcaggc cactcaatat caggtgcagt
       61 tgatgtatca acacgattca acaatacccg atatttattc catgcctcca gcaacgatct
      121 ttcttcctcc gttgcgattt ccagatctac agcatcctgc agtggcgcaa tatactcact
      181 aaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgctgtat
      241 cgaagcatac taggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat
      301 tcatcgccat aattgattta attcacaaat aaaactataa catggtgaaa tcaatgaaaa
//
LOCUS       DAQSWQ010000365          357 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.denovo.368, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000365 DAQSWQ010000000
VERSION     DAQSWQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.denovo.368"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            <1..>357
                     /locus_tag="R8G18_004560"
     rRNA            <1..>357
                     /locus_tag="R8G18_004560"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           95 a           82 c          105 g           75 t
ORIGIN      
        1 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
       61 gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa
      121 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      181 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
      241 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
      301 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAQSWQ010000366        16359 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.guided.091, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000366 DAQSWQ010000000
VERSION     DAQSWQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.guided.091"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            26..1159
                     /locus_tag="R8G18_004561"
                     /pseudo
     CDS             26..1159
                     /locus_tag="R8G18_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            1248..1781
                     /gene="blc"
                     /locus_tag="R8G18_004562"
     CDS             1248..1781
                     /gene="blc"
                     /locus_tag="R8G18_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HEE9798046.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1778..2095)
                     /gene="sugE"
                     /locus_tag="R8G18_004563"
     CDS             complement(1778..2095)
                     /gene="sugE"
                     /locus_tag="R8G18_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEE9798047.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2270..2416)
                     /gene="ecnB"
                     /locus_tag="R8G18_004564"
     CDS             complement(2270..2416)
                     /gene="ecnB"
                     /locus_tag="R8G18_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HEE9798048.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2527..2652)
                     /gene="ecnA"
                     /locus_tag="R8G18_004565"
     CDS             complement(2527..2652)
                     /gene="ecnA"
                     /locus_tag="R8G18_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HEE9798049.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2704..3270)
                     /gene="efp"
                     /locus_tag="R8G18_004566"
     CDS             complement(2704..3270)
                     /gene="efp"
                     /locus_tag="R8G18_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEE9798050.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3312..4340
                     /gene="epmB"
                     /locus_tag="R8G18_004567"
     CDS             3312..4340
                     /gene="epmB"
                     /locus_tag="R8G18_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HEE9798051.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            4730..5599
                     /locus_tag="R8G18_004568"
     CDS             4730..5599
                     /locus_tag="R8G18_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HEE9798052.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(5802..6155)
                     /locus_tag="R8G18_004569"
     CDS             complement(5802..6155)
                     /locus_tag="R8G18_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HEE9798053.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(6293..7939)
                     /gene="groL"
                     /locus_tag="R8G18_004570"
     CDS             complement(6293..7939)
                     /gene="groL"
                     /locus_tag="R8G18_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEE9798054.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(7983..8276)
                     /locus_tag="R8G18_004571"
     CDS             complement(7983..8276)
                     /locus_tag="R8G18_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEE9798055.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            8552..9808
                     /gene="yjeH"
                     /locus_tag="R8G18_004572"
     CDS             8552..9808
                     /gene="yjeH"
                     /locus_tag="R8G18_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HEE9798056.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(9830..10300)
                     /gene="fxsA"
                     /locus_tag="R8G18_004573"
     CDS             complement(9830..10300)
                     /gene="fxsA"
                     /locus_tag="R8G18_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEE9798057.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            10637..12073
                     /gene="aspA"
                     /locus_tag="R8G18_004574"
     CDS             10637..12073
                     /gene="aspA"
                     /locus_tag="R8G18_004574"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEE9798058.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            12191..13492
                     /gene="dcuA"
                     /locus_tag="R8G18_004575"
     CDS             12191..13492
                     /gene="dcuA"
                     /locus_tag="R8G18_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HEE9798059.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            13608..13946
                     /gene="cutA"
                     /locus_tag="R8G18_004576"
     CDS             13608..13946
                     /gene="cutA"
                     /locus_tag="R8G18_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HEE9798060.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            13922..15619
                     /gene="dsbD"
                     /locus_tag="R8G18_004577"
     CDS             13922..15619
                     /gene="dsbD"
                     /locus_tag="R8G18_004577"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEE9798061.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            15656..16231
                     /locus_tag="R8G18_004578"
     CDS             15656..16231
                     /locus_tag="R8G18_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEE9798062.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         4008 a         4206 c         4037 g         4108 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 tactgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      421 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc tagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
      661 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
      721 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
      781 caatttaacc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
      901 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
      961 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1021 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1261 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc
     1321 cgcgtggcgt gacagtagtg aataatttcg acgctaaacg ctatctcggt acctggtatg
     1381 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1441 gtctgcgtga tgacggcggc ctgaatgtca tcaataaagg ctataaccct gacagagaaa
     1501 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaacacgc gctgcgctga
     1561 aagtgtcatt ctttggtcct ttctatggcg gttataacgt cattgcactt gatcgggaat
     1621 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     1681 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1741 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1801 caataatccc caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcac
     1861 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     1921 aagccgtccc taccggtaac gatttcatcg cccaggcaag tagcgccata ctgactatca
     1981 tcgccgtcac agtaataaca cttggcgtca aacgactaaa gccgtgggta tatttcaggc
     2041 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2101 gctccggaga attggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     2161 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttgtga
     2221 ctatcgcaaa aaaacaaacg gcacgacata actgtcgtac cgttattgct tattgctgcg
     2281 ctttcgttgc tgcaccagaa atcgcgttac cgccatcaga aatgtcttca ccaacgccac
     2341 gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag aacagaaaag atcgctgcaa
     2401 ttgtcttctt caccataacg tcttcctttt aggtaatgtt atttatgttt gcctatagca
     2461 aattaataat agacaacatc gccaaaaatg atggaaatct gatgattttt cggaagagaa
     2521 gaaaaattag ctggcagcgc gggagatgga gttgccgaga tgtttgatgt cttcgccgaa
     2581 accgcgagcg gtgttacagc ccgtgagcag cgtgctggca agcaaaacaa gaacgataag
     2641 gcgtttcatc atctctgacc tgaataagtg atggtgcagc ctgcaggccg caccacaacc
     2701 gcattacttc acgcgagaga cgtattcacc agagcgggta tccactttga tgacttcgcc
     2761 gatttgtacg aacagcggaa ctttaaccac agcgccagta gacagggtag ccggtttgcc
     2821 gccagtacct gcggtatcac ctttcaggcc cggatcggta tcaacgattt ccagttcaac
     2881 gaagttcggc ggagtaacgg agatcggctg accattccac agagttacga tacactctgc
     2941 ctgatccagc agccatttag cgttgtcgcc aattgctttt gcatcagcag acagctgctc
     3001 gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg ttgtacaggt aagtcaggtt
     3061 catatcgaca acatcagcgc cttcagcgga atcagtagat ttgaaggttt tttctacgcg
     3121 agtaccggtc agcagacgac gcagtttaac gcgagcaaat gcctggcctt tacccggttt
     3181 tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct aacatgattt taagaccagc
     3241 acgaaaatcg ttgctatagt acgttgccat aaggccctct gaaatttgtt aattggtagc
     3301 taagccacaa aatggcgcat attgtaaccc taaatacccc atccagagaa gattggttaa
     3361 cgcaacttgc cgatgttgtg accgatcctg gtgaacttct gcgtcttttg aatatagacg
     3421 cggacgaaaa actgttggcc ggacgcagcg ccaaaaagct gtttgccctg cgtgtgcccc
     3481 gctcatttat cgatcgcatg gagaaaggca atccggacga tcctattttg cgtcaggtac
     3541 ttacctcgca agatgagttt gtcgtcgcgt ccggattctc caccgaccca ctggaagaac
     3601 agcacagcgt agtgcctggt ttgttgcata aataccacaa ccgggcgctt ttgctggtca
     3661 aaggcggctg cgcggtaaat tgccgctatt gcttccgtcg ccacttcccc tatgccgaaa
     3721 atcagggcaa caagcgtaac tggcagactg cacttgagta tgttgctgcg catccggaac
     3781 tggacgagat gattttctcc ggcggcgatc cgctgatggc gaaagatcac gagctggact
     3841 ggttgctcac acaactggaa gccatcccgc atatcaaacg tctgcggatt cacagccgtc
     3901 tgccgattgt gatcccggca cgtatcaccg aggcgctggt tgaacgcttt gcccgttcta
     3961 cgctgcaaat cttgctggtg aatcacatca atcatgccaa tgaggtagat gaaacgttcc
     4021 gtcaggcgat ggctaaattg cgccgtgtcg gtgtcaccct gcttaaccag agcgttctgt
     4081 tacgtggtgt gaacgataac gcacaaacgt tggcaaacct gagtaatgcg ttgttcgatg
     4141 ccggcgtgat gccgtattac ctgcatgtgc tagataaagt acagggcgcg gcgcatttta
     4201 tggtgagtga tgacgaagca cggcagatta tgcgtgagtt gctgacattg gtgtcgggat
     4261 atctggtgcc gaaactggcg cgagaaattg gcggcgaacc cagcaaaacg ccgctggatc
     4321 tccagctacg ccagcagtaa ctgattaaac agggaagtta aacattagcc tgatgcgcta
     4381 cgcttatcag gcctacattg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     4441 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     4501 taaactggga aatttaatct ttgcccgttg cataaccttt aattattcat atacattgct
     4561 gttgttactc tttatatagc caacaccaat aattaacctt tcttttctgt ttatatagag
     4621 tgagtacggc tatttatgtt attttaacaa ctattactac caagcccatt tatctggctt
     4681 ataaattaaa attaattatt taattgagaa atggttaggg agaacctaca tggccataag
     4741 catcaagggt gttaataccg gtgttattcg caagagtaat aatttcattg cactggcgct
     4801 gaaaattaaa gaacctcgca ataaagaatc gttgttcttt atgtctgtca tggaactgcg
     4861 cgacctgctg attgcgctgg aaagtcgtct gcatcaaaag cataaactga atgctgctgc
     4921 acgccttcag tatgagcaag ctcgcgataa agtcattaag aaaatggcag aaaacatccc
     4981 agaaattctg gttgatgagc ttaaaaatgc cgatattaat cgtcgggtta acacgctgga
     5041 acttaccgat aatcaaggcg aaaatcttac cttcgtatta acgctacatg acggtagcaa
     5101 atgtgagtta gtcgtcaatg agttgcagat tgaaatgctg gcgcgagcaa tcattcatgc
     5161 aattaataat gcggaaatgc gtgaactggc attacgtatt acctcactgt tagatttcct
     5221 gccgttatac gacgttgact gccaggacaa tggcaatctg gaatatgaca cctattccca
     5281 accggaatgg aaacataacc tgttcgacca ttatctggcg gtgctttacc gctttaaaga
     5341 tgaaagtggc aaagaacaat ttagcggcac agtggtgaaa acgcgtgaag caacgccggg
     5401 taaagaaata gaggcgatca ctcgccgaat gcttgatttc agcccacgac tgaaaaaact
     5461 cgcgggtgtg ccatgtcagg tctatgttcg caccgtagcg gcgaataacg cccaaccgct
     5521 aacccaggat caatgcttac gcgcgctgca tcacttacgt gttcaatcca ccagcaaaac
     5581 tgcaccacag gcgaaataat ctcatctttg ctaatgcgag gctggagcca tccagcctga
     5641 aatgactgat aacgtgatcg ttggtcatgc cggatgcgac gtaaacgcct tatccggcct
     5701 acaaattctc aagcattcaa tcagttgcaa tgcctgctgt aggcctgata agcgaagcgc
     5761 atcaggcaat aatacgtttg tcacccagcc tctggatcac atcagttcgg gcacttataa
     5821 acctgaccga taatctggct atccgtcggg acaaaactgg acaacattcc ctgcgacggg
     5881 ctgctgatgc cataaatcac gttaccgccc attgcagccg cctggttgcg cagatcgttt
     5941 gctgcgccgc gcatagaacc gccctcttct ccgtgttgcc cggaaagcca gttgctttgc
     6001 ttacctgtcg cagtaccaat cagctggcac tctgcgcctg gctgctcgtc cacaatgcgt
     6061 acactctgac cggcagcact caattcgttg ctggagctac aacccgccat cagtagcgcg
     6121 gcaccgacaa tccctgctaa gtattttacg tgcatgttat tccccataat caatgagctg
     6181 gacgccactc gcgtcgtccg tgtctgaatc ttatactaga aagatgacca aaagaaaaac
     6241 ccccagacat ttctgcccgg gggtttgttt atttctgcga ggtgcagggc aattacatca
     6301 tgccgcccat gccacccatg ccgcccatac cgccagcagc gcctaagtca gctgcatcat
     6361 ttttcggcag gtcggtaacc atgcactcgg tggtgatcat caggccagcc acagaagccg
     6421 cgtactgcag agcagaacgg gttactttgg ttgggtccag gatacccatg tcgatcatgt
     6481 tgccgtattc ttcggttgct gcgttgtaac cgtagttgcc gtcgccgcct ttaacggtgt
     6541 tagcaacaac agacggttct tcgccgcagt tcaggacgat ctgacgcagc ggagcttcca
     6601 ttgcacgcag tgcaactttg atacccacgt tctggtcttc gttctgacca cgcaggtcag
     6661 ccagtttaga cgctacgcgg atcagcgcaa caccaccacc agcaaccacg ccttcttcta
     6721 ccgcagcacg ggtcgcgtgc agggcgtctt caacgcgtgc ttttttctct ttcatttcaa
     6781 cttcggtagc agcacctact ttgataactg caacgccgcc tgccagtttc gctacgcgtt
     6841 cctgcagttt ttcacggtcg tagtcagaag ttgcttcttc aatctgctga cggatctgag
     6901 caacacggcc ctggattgca gcttcttcac ccacgccatc gatgatagtg gtggtgtctt
     6961 tgttgatcac aacacgttta gcctgaccca ggtcttccag ggttgctttt tccagttcca
     7021 taccgatctc ttcagagatc acggtaccgc cagtcagggt tgcgatatcc tgcagcatag
     7081 ctttacgacg atcgccgaag cccggagctt taactgcagc aactttcacg atgccacgca
     7141 tggtgttaac aaccagagtt gccagcgctt cgccttctac atcttcagcg atgatcagca
     7201 gcggtttgcc tgctttggca acggcttcca gaaccggcag catttcgcgg atgttggaga
     7261 ttttcttgtc agccagcagg atgaacgggc tttccagttc tactgcgcca gtttccggct
     7321 tgttgatgaa gtaaggagac aggtagccac ggtcgaactg cataccttca accacgtcca
     7381 gttcgtcctg cagaccggta ccgtcttcaa cggtgataac gccttcttta ccgactttgt
     7441 ccatcgcttc agcgatcagt ttacctacgg tttcgtcgga gttagcggag atggtaccaa
     7501 cctgagcaat cgctttagag tcagagcacg gtacggacag cgctttcagt tcttcaactg
     7561 cagcggtaac ggctttgtcg ataccacgtt tcaggtccat cgggttcatg cccgcagcaa
     7621 cagctttcag accctcagtg atgatagcct gagccagtac agttgcagtg gtggtaccgt
     7681 cgcctgcagc gtcgttcgct ttagaggcaa cttctttcac catctgcgca cccatgtttt
     7741 cgaacttgtc ttccagttcg atttcacgag caacggaaac accatctttg gtgatggtcg
     7801 gtgcaccgaa agatttatcc agaactacgt tacggccttt cggaccgagg gtaactttca
     7861 ctgcatctgc cagtacgttt acgccgcgca gcattttcac acgagcgtcg ttaccgaatt
     7921 ttacgtcttt agctgccatt atctttattc cttaaattcg tatgttcagt gtcgtgcgag
     7981 gattacgctt caacaattgc cagaatgtcg ctttcggaca tgatcaacac ttcttcattg
     8041 tcgatcttct cagatttcac accgtagcca tcgttgaaaa taacgatgtc gccaactttc
     8101 acatccagcg gcttcacttc gccattttca aggatacggc cattgccgac agccagcact
     8161 tcgccgcggg tggatttagc cgctgcagag ccggtcagaa cgatgccgcc agcagattta
     8221 gtttcaactt ctttacgctt gacgatcacg cgatcatgca atggacgaat attcattgat
     8281 aactctcctt tgagaaagtc cgtatctgtt atgggtgacg ccggagctta cgtggtttcc
     8341 cggctggtga ccagagaaat ggggatgagg attcgcccct tcaaggggga aaaagaaaaa
     8401 aaattctgat cagcacaaaa tcgggtgaaa accctgattc acctcacatt tcatcgcaat
     8461 ttcttcatcg ccgtcataag cgaatctgat tgtgctacca tcgaaaatct acgcagttgc
     8521 ccaaaatttg ggcgcaatcg gatatcaacc catgagtgga ctcaaacaag aactggggct
     8581 ggcccagggc attggcctgc tatcgacgtc attattaggc accggcgtgt ttgccgttcc
     8641 tgcgttagct gcacaggtag caggcaataa cagcctgtgg gcgtggcccg ttttgattat
     8701 cttagtgttc ccgattgcga ttgtgtttgc gattctgggt cgccactatc ccagcgcagg
     8761 cggtgtcgcg cacttcgtcg gtatggcgtt tggttcgcgg cttgagcgag tcaccggctg
     8821 gttgttttta tcggtcattc ccgtgggttt gcctgccgcg ctacaaattg ccgccgggtt
     8881 cggccaggca atgtttggct ggcatagctg gcaactgttg ttggcagaac tcggtacgct
     8941 ggcgctggtg tggtatatcg gtactcgcgg tgccagttcc agtgctaatc tacaaaccgt
     9001 tattgccgga cttatcgtcg cgctgattgt cgctatctgg tgggcgggcg atatcaaacc
     9061 tgcgaatatc cccttcccgg cacctggtaa tatcgaactt accgggttat ttgctgcgtt
     9121 atcagtgatg ttctggtgtt ttgtcggtct ggaagcattt gcccatcttg cctcggaatt
     9181 taaaaatcca gagcgtgatt ttcctcgcgc attgatgatt ggtctgctgc tggcaggatt
     9241 agtctactgg ggctgtacgg tagtcgtctt acacttcgac gcctatggtg aacaaatggc
     9301 cgcggcagca tcgcttccaa aaattgtagt gcaactgttc ggtgtaggag cgttatggat
     9361 tgcctgcgta attggctatc tgacctgctt tgccagtctc aacatttata tacagagctt
     9421 cgcccgcctg gtctggtcgc aggcgcaaca taatcctgac cactacctgg cacgcctctc
     9481 ttctcgccat atcccgaata atgccctcaa tgcggtgctc ggctgctgcg tggtgagcac
     9541 tttggtgatt catgctttag agatcaatct ggacgctctt attatttatg ccaatggcat
     9601 ctttattatg atttatctgt tatgcatgct ggcaggctgt aaattattgc aaggacgtta
     9661 tcgactactg gcggtggttg gcgggctgtt atgcgttctg ttactggcaa tggtcggctg
     9721 gaaaagtctc tatgcgctga ttatgctggc ggggttatgg ctgtttctgc caaaacgaaa
     9781 aacgccggaa aatggcataa ccacataatc cggcgtttcg acattaatcc tagcgatcgt
     9841 ctttatgatc aaggcggtcg cggtcatcat cctttcgctg gtactcacca tcaaaagtat
     9901 taccgccacc ggtcccggcg ctaaaaccgc cgcctggcat gcgagaaaag cgcaaatgtg
     9961 gcatcaactt cactgtcaga tgcttttgca ccggcggcaa taaaagcaga agaccgagga
    10021 agtcggtgaa aaagcccggt aataaaagca gcaaaccggc aataatcagc gaaacacttt
    10081 taatcatctc cgccgcaggg ttttcacccg ccgccatttt ttgctgcatc agcacgaaat
    10141 tcttaaagcc ctggttacgt accagtgaca taccgataac tgacgtgaat ataaccagca
    10201 cgagggtcag caataccccc aatacatggg caacctgaat aaagattgaa atctcaatat
    10261 agacataaag gaaaatggca ataaaaggta accagcgcaa aggtttctcc tgtaatagca
    10321 gccggttaaa cccggctacc tgaatgggtt gcgaatcgcg ttcagcttat attgtggtca
    10381 ttagcaaaat ttcaagatgt ttgcgcaact atttttggta gtaatcccaa agcggtgatc
    10441 tatttcacaa attaataatt aaggggtaaa aaccgacact taaagtgatc cagattacgg
    10501 tagaaatcct caagcagcat atgatctcgg gtattcggtc gatgcagggg ataatcgtcg
    10561 gtcgaaaaac attcgaaacc acatatattc tgtgtgttta aagcaaatca ttggcagctt
    10621 gaaaaagaag gttcacatgt caaacaacat tcgtatcgaa gaagatctgt tgggtaccag
    10681 ggaagttcca gctgatgcct actatggtgt tcacactctg agagcgattg aaaacttcta
    10741 tatcagcaac aacaaaatca gtgatattcc tgaatttgtt cgcggtatgg taatggttaa
    10801 aaaagccgca gctatggcaa acaaagagct gcaaaccatt cctaaaagtg tagcgaatgc
    10861 catcattgcc gcatgtgatg aagtcctgaa caacggaaaa tgcatggatc agttcccggt
    10921 agacgtctac cagggcggcg caggtacttc cgtaaacatg aacaccaacg aagtgctggc
    10981 caatatcggt ctggaactga tgggtcacca gaaaggtgaa tatcagtacc tgaacccgaa
    11041 cgaccatgtt aacaaatgtc agtccactaa cgacgcctac ccgaccggtt tccgtatcgc
    11101 agtttactct tccctgatta agctggtaga tgcgattaac caactgcgtg aaggctttga
    11161 acgtaaagct gtcgaattcc aggacatcct gaaaatgggt cgtacccagc tgcaggacgc
    11221 agtaccgatg accctcggtc aggaattccg cgctttcagc atcctgctga aagaagaagt
    11281 gaaaaacatc caacgtaccg ctgaactgct gctggaagtt aaccttggcg caacagcaat
    11341 cggtactggt ctgaacacgc cgaaagagta ctctccgctg gcagtgaaaa aactggctga
    11401 agtcactggc ttcccatgcg taccggctga agacctgatc gaagcgacct ctgactgcgg
    11461 cgcttatgtt atggttcacg gcgcgctgaa acgcctggct gtgaagatgt ccaaaatctg
    11521 taacgacctg cgcttgctct cttctggtcc acgtgccggc ctgaacgaga tcaacctgcc
    11581 ggaactgcag gcgggctctt ccatcatgcc agctaaagta aacccggttg ttccggaagt
    11641 ggttaaccag gtatgcttca aagtcatcgg taacgacacc actgttacca tggcagcaga
    11701 agcaggtcag ctgcagttga acgttatgga gccggtcatt ggccaggcta tgttcgaatc
    11761 cgttcacatt ctgaccaacg cttgctacaa cctgctggaa aaatgcatta acggcatcac
    11821 tgctaacaaa gaagtgtgcg aaggttacgt ttacaactct atcggtatcg ttacttacct
    11881 gaacccgttc atcggtcacc acaacggtga catcgtgggt aaaatctgtg ccgaaaccgg
    11941 taagagtgta cgtgaagtcg ttctggaacg cggtctgttg actgaagcgg aacttgacga
    12001 tattttctcc gtacagaatc tgatgcaccc ggcttacaaa gcaaaacgct atactgatga
    12061 aagcgaacag taatcgtaca gggtagtaca aataaagaag gcacgtcaga tgacgtgcct
    12121 tttttcttgt gagcagtaac ttaaaaataa caatctaata tcaacttgtt aaaaaacaag
    12181 gaaggctaat atgctagttg tagaactcat catagttttg ctggcgatct tcttgggcgc
    12241 cagattgggg ggaataggta ttggttttgc aggcggattg ggggtgctgg ttcttgccgc
    12301 tattggcgtt aaacccggta acatcccgtt cgatgtcatt tccattatca tggcggttat
    12361 cgccgctatt tctgccatgc aggttgctgg cggtctggac tatctggttc atcagacaga
    12421 aaagctgctg cgccgtaacc cgaaatacat cacaatcctc gcaccgatcg tgacctattt
    12481 cctgacaatc tttgctggta ctggcaacat ctctctggcg acactgccag ttatcgctga
    12541 agttgcgaag gaacaaggcg ttaaaccttg ccgtccgctg tctactgcag tggtatccgc
    12601 gcagattgcg atcaccgcat cgccaatctc agcggcagtg gtttacatgt cttccgtgat
    12661 ggaaggtcat ggcatcagct acctccatct gctctccgtg gtcatcccgt ccaccctgct
    12721 ggcggttctg gtgatgtcct tcctggtcac tatgctgttc aactccaaac tctctgacga
    12781 tccgatttat cgcaagcgtc tggaagaggg cctggttgaa ctgcgcggtg aaaagcagat
    12841 tgaaatcaaa tccggtgcaa aaacgtccgt ctggctgttc ctgctgggcg tagttggcgt
    12901 ggttatctat gcaatcatca acagcccaag catgggtctg gttgaaaaac cgctgatgaa
    12961 caccaccaac gcaatcctga tcatcatgct cagcgttgca actctgacca ccgttatctg
    13021 taaagtcgat accgacaaca tcctcaactc cagcaccttc aaagcaggta tgagcgcctg
    13081 tatttgtatc ctgggtgttg cgtggctggg cgatactttc gtttccaaca acatcgactg
    13141 gatcaaagat accgctggtg aagtgattca gggtcatccg tggctgctgg ccgtcatctt
    13201 cttctttgct tctgctctgc tgtactctca ggctgcaacc gcaaaagcac tgatgccgat
    13261 ggctctggca ctgaacgttt ctccgctgac cgctgttgct tctttcgctg cggtgtctgg
    13321 tctgttcatt ctgccgacct atccgacgct ggttgctgcg gtacagatgg atgacacggg
    13381 tacaacccgt atcggtaaat tcgtcttcaa ccatccgttc ttcatcccgg gtactctggg
    13441 tgttgccctg gccgtttgct tcggcttcgt gctgggtagc ttcatgctgt aatgaccaat
    13501 cgcggggcgt tcacgccccg ctttctttcc cgccgactaa catccttccc ccgtccgttg
    13561 tatagtgacc tctctcttgc ggttccatct gttcttgcga ggtgtttatg cttgatgaaa
    13621 aaagttcgaa taccgcgtct gtcgtggtgc tatgtaccgc accggatgaa gcgacagccc
    13681 aggatttagc cgccaaagtg ctggcggaaa aactggcggc ctgcgcgacc ttgatccccg
    13741 gcgctacctc tctctattac tgggaaggta agctggagca agaatacgaa gtgcagatga
    13801 ttttaaaaac taccgtatct caccagcagg cactgctgga atgcctgaag tctcatcatc
    13861 catatcaaac cccggaactt ctggttttac ctgttacaca cggagacaca gattacctct
    13921 catggctcaa cgcatcttta cgctgatcct gctactttgc agcacttccg tttttgccgg
    13981 attattcgac gcgccgggac gttcacattt tgtccccgcg gatcaagcct ttgcttttga
    14041 ttttcagcaa aaccaacatg acctgaatct gacctggcag atcaaagacg gttactacct
    14101 ctaccgtaaa cagatccgca ttacgccgga acacgcgaaa attgccgacg tgcagctgcc
    14161 gcaaggcgtc tggcatgaag atgagtttta cggcaaaagc gagatttacc gcgatcggct
    14221 gacgcttccc gtaaccatca accaggcgag tgcgggagcg acgttaactg tcacctacca
    14281 gggctgtgct gatgccggtt tctgttatcc gccagaaacc aaaaccgttc cgttaagcga
    14341 agtggtcgcc aacaacgcag cgtcacagcc tgtgtctgtt ccgcagcaag agcagcacac
    14401 cgcgcaattg cccttttccg cgctctgggc gttgttgatc ggtattggta tcgcctttac
    14461 gccatgcgtg ctgccaatgt acccactgat ttctggcatc gtgctgggtg gtaaacagcg
    14521 gctctccact gccagagcat tgttgctgac ctttatttat gtgcagggga tggcactgac
    14581 ttacacggcg ctgggtctgg tagttgccgc cgcagggtta cagttccagg cggcgctaca
    14641 gcacccatac gtgctcattg gcctcgccat cgtctttacc ttgctggcga tgtcaatgtt
    14701 tggcttgttt actctacaac tcccctcttc gctgcaaaca catctcacat tgatgagcaa
    14761 tcgccaacag ggcggctcac ctggcggtgt gtttgttatg ggggcgattg ccggactgat
    14821 ctgttcacca tgcaccaccg caccgcttag cgcgattctg ctgtatatcg cccaaagcgg
    14881 gaacatgtgg ctgggcggcg gcatgcttta tctctatgcg ttgggcatgg gcctgccgct
    14941 gatgctaatt accgtctttg gtaaccgctt gctgccgaaa agcggcccgt ggatggaaca
    15001 agtcaaaacc gcgtttggtt ttgtgatcct cgcactgccg atcttcctgc tggagcgagt
    15061 gattggtgat atatggggat tacgcttgtg gtcggcgctg ggtgtcgcat tctttggctg
    15121 ggcctttatc accagcctac aggccaaacg cggctggatg cgcgtggtgc aaataatcct
    15181 gctggcagcg gcattggtta gcgtgcgccc acttcaggat tgggcatttg gtgcgacgca
    15241 taccgcgcaa actcagacgc atctcaactt tacacaaatc aaaacggtag atgagttaaa
    15301 tcaggcgctc gttgaagcca aaggcaaacc ggtgatgtta gatctttatg ccgactggtg
    15361 cgtcgcctgt aaaaagtttg agaaatacac cttcagcgac ccgcaggtgc aaaaagcgtt
    15421 agcagacacg gtcttacttc aggccaacgt cactgctaac gacgcacaag atgtggcgct
    15481 gttaaagcat cttaatgtcc ttggactacc gacaattctc ttttttgacg gacaaggcca
    15541 ggagcatcca caagcacgcg tcacgggctt tatggatgct gaaaccttca gcgcacattt
    15601 gcgcgatcgc caaccgtgaa cgacacttgc agtgggaaag acggaggaaa atagcgtgca
    15661 acgtgaagat gtactgggag aagccctgaa attattagaa ttacaaggga ttgccaacac
    15721 cacgctggag atggttgctg aacgtgtgga ttatccactg gacgagctac gccgcttctg
    15781 gccagacaaa gaggcaatcc tctacgatgc gctgcgttat cttagccaac agatagatgt
    15841 ctggcgtcgt cagctgatgc ttgacgaaac gcaaaccgcc gaacaaaagc tgctggcacg
    15901 ttatcaggcg ttatcggagt gcgttaaaaa caaccgctat ccgggctgtc tatttatcgc
    15961 tgcctgtacg ttttatcccg accctggcca ccctattcat caactggccg atcagcaaaa
    16021 aagcgcggcc tacgatttca cccacgaact gttaaccacg ctggaagttg acgatccggc
    16081 gatggtagca aagcagatgg aactggtgct ggaaggctgt ttaagccgaa tgctggtgaa
    16141 tcgtagccag gcggatgtcg acaccgcaca tcggctggcg gaagatatcc tgcgcttcgc
    16201 ccgctgccgc cagggtggtg cactgacctg acagaaacac agaaaagaag cgatttgccg
    16261 caatcttaag cagttgaatc gcttttactg aaattaggtt gacgagatgt gcagattacg
    16321 gtttaatgcg ccccgttgcc cggatagctc agtcggtag
//
LOCUS       DAQSWQ010000367        11551 bp    DNA     linear   BCT 01-NOV-2023
DEFINITION  TPA_asm: Shigella sonnei strain CMCC51592 isolate human
            SAMN37717590-rid19967913.guided.127, whole genome shotgun sequence.
ACCESSION   DAQSWQ010000367 DAQSWQ010000000
VERSION     DAQSWQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37717590
            Sequence Read Archive: SRR26320974
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001955356.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001955356.1
            Genome Coverage       :: 398x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/27/2023 21:53:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,589
            CDSs (total)                      :: 4,495
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 113 of 242
            Pseudo Genes (incomplete)         :: 66 of 242
            Pseudo Genes (internal stop)      :: 84 of 242
            Pseudo Genes (multiple problems)  :: 21 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37717590-rid19967913.guided.127"
                     /strain="CMCC51592"
                     /isolate="human"
                     /isolation_source="feces"
                     /db_xref="taxon:624"
                     /country="China"
                     /lat_lon="23.14 N 113.30 E"
                     /collection_date="2005-01-01"
                     /collected_by="Wang tianming"
     gene            1..652
                     /locus_tag="R8G18_004579"
                     /pseudo
     CDS             1..652
                     /locus_tag="R8G18_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /locus_tag="R8G18_004580"
     CDS             649..1263
                     /locus_tag="R8G18_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HEE9798063.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="R8G18_004581"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="R8G18_004581"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HEE9798064.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="R8G18_004582"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="R8G18_004582"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEE9798065.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="R8G18_004583"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="R8G18_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HEE9798066.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="R8G18_004584"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="R8G18_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HEE9798067.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="R8G18_004585"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="R8G18_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HEE9798068.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6673..7776
                     /gene="proB"
                     /locus_tag="R8G18_004586"
     CDS             6673..7776
                     /gene="proB"
                     /locus_tag="R8G18_004586"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEE9798069.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7788..9041
                     /gene="proA"
                     /locus_tag="R8G18_004587"
     CDS             7788..9041
                     /gene="proA"
                     /locus_tag="R8G18_004587"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEE9798070.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDCG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9156..9231
                     /locus_tag="R8G18_004588"
     tRNA            9156..9231
                     /locus_tag="R8G18_004588"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9189..9191,aa:Thr,seq:cgt)
     gene            9837..9995
                     /locus_tag="R8G18_004589"
     CDS             9837..9995
                     /locus_tag="R8G18_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEE9798071.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2998 a         2684 c         2915 g         2954 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgatctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     6481 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     6541 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt
     6601 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6661 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     6721 acaggcggat cgcgccgtct gaaccgtgcc catattgttg aacttgttcg ccagtgcgcg
     6781 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     6841 cgtgagcacc tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggca
     6901 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     6961 cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac
     7021 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     7081 gatgctgtcg ctacggcaga gattaaagtc ggcgataacg ataacctttc tgcgctggcg
     7141 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     7201 gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     7261 gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtacc
     7321 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     7381 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     7441 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
     7501 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
     7561 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     7621 aacctcgaag gccgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcattacgc
     7681 cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
     7741 gttgccgttc accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa
     7801 tgggcattgc tgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
     7861 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
     7921 ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc
     7981 gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca
     8041 acctcgccga tccggtgggg caggtaatcg atggcggcgt actggactgc ggcctgcgtc
     8101 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
     8161 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgcggtg
     8221 gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt gattcaggac gccctgaaat
     8281 cctgcggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     8341 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     8401 tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat
     8461 gccatattta cgttgatgaa agtgtagaga tcgctgaagc attaaaagtg atcgtcaacg
     8521 cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca
     8581 tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac
     8641 acgcagatgc agctgcactg gcgcagttgc aggcaggccc tgcgaaggtg gttgctgtta
     8701 aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg
     8761 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     8821 tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     8881 acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggtctgggt gcggaagtgg
     8941 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     9001 acaagtggat cggcattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa
     9061 aagtagccat ttgattcaca aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     9121 accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag ttggtagagc
     9181 agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc attaagaaaa
     9241 ttaataactt agcttaactt ctctaagaaa ttgtatcttc tgttatcact tacaaacgcc
     9301 tatacgttgt tatgggcttt ggtagtgtaa cacgtatggg cgagtagttc ccaggctcaa
     9361 cctcgtaaca agttcctttg aagctggaag acgtgccacc gtctctaaac cttctaccaa
     9421 aactcttagc aagatgttcc acacctggcg cggtgtccaa tgatgctaca cgtcgtgaga
     9481 cgttgcagag gataaacagg ctaaatgatg ggaagagttt taagtgcaat agtggattaa
     9541 agcccacctc ttcaaaacct aacttgatcg ggaaagaatc aagctctagc acggtttcgt
     9601 ccaccggagt acaacgggtg cctgataaga gaagaactgc taagacttca tagcttttgt
     9661 gaaaaagccc tttttagtgg atacgcctac ggcgcttatt ttttggtata accatcaaag
     9721 gcgctttatg gctcctattg tcttgaaaat gtatctttac ttgtattgtg ttttatgcgt
     9781 agtctccctg tgtagtatgt aacttgttaa ttttcatatg aatacagagg cacacgatga
     9841 aaaacattgc agcaatcaaa cgtaataacc gcaagattca cgctcgtaag ttcctgtcta
     9901 cgccagaagg aaaagcctgg ctagagcgta aacagagaga gaacgaagaa agaaaactcc
     9961 ttagtgagtt gaaatggctt agagaggatt tctgaaaatc tccagaacgc aacaggacgc
    10021 attctaacgc tgttttcatt actcggtata gggataggtg atgtgtgttt aatgcgtctg
    10081 tgcgcttgtt ttgtgcctta ttttgcattt tcataatgcg gaagagaaca cggaaaacct
    10141 taaaattaat tattggggta tttaaggcta ttttcaggtg atttaagggt attaaacttg
    10201 attaaaatat aaacagataa ttatgtatct tattgattta taaggttatt tttaagtaaa
    10261 ggtaaataaa aaataaatag ccataaatag ctataaatac atcttaatac ctatatccct
    10321 tcccttatgg ctagattgcg agaggtgctt aacgcgattt tcagcgcccc ccgatctgga
    10381 aaatccagaa tccccaaata tcagcaagga attttcttgc gtaatcaaca tcgtttttac
    10441 ctattaaatc attacacaga aagcctttct gtaatgtctc tcactaaact ttcactaaac
    10501 acgagaatga tcggttttat gtcggatcag atgctagaaa cagcaccacg tctcacaagg
    10561 gctgtaagcg atgaaaccag tgtttatgcg ggtgcaggtc aaaatatagg ccaaaatcca
    10621 cttaacataa taaatgttat atgtgctaag gaacacatat aacgccttga attgatgtat
    10681 caggggaaaa gtgatggatt ttttacgtag gagcacgaaa gggcttctat tgacttttgt
    10741 atgctatgaa atataattaa agttttttgt ttttattgtg tgatgtggtg ggttacacag
    10801 gggaatagat taaatggtgt tctgtttata caggtttatt tgttagatca acaatttggc
    10861 atttctaaac agatgtttga acgttgccaa atgtcttgac aacacaagaa aagggcttgc
    10921 tatgccaggc attacacgaa ataaggctat aaaaacaaaa taaattttaa aatttgcaca
    10981 aacgagaaac tcgttcagaa acaagcagaa caatttttag ccaacaattt ctgatggtgc
    11041 tctgtaagcc gcattacaca aggctttgcg aaagtgatgt aaaagtgaag aaagtaagaa
    11101 ttgaactttt aagctacttt tcattggatt ttttagtagg gaattacaca gggaatagag
    11161 agcattattc tatgatttac acagggaata tagggggatt atggcacaca catagaaaga
    11221 cattacacag agtgctatgt gacaaatgga tgtaacgaga ggttacattc atttatctga
    11281 aatgctctct gtaagaaagt tggtagtaac gatatgttac atagactaat tattgaatgt
    11341 aacgatatta cacatcggta gtaacgatac gttacatcgg aagatgattg gatgtaacgg
    11401 tagctcacag tgaactaggg gtaggtcata gtgaactacc cccacagaat tctgttaccc
    11461 ctatcaagtc tgatacccct atcaaaactg atacccgata agtataaata acacctaaat
    11521 agctgcgcgg aatagtagat cactttgagg g
//
